LOCUS       DAXHNE010000001        49823 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.001, whole genome shotgun sequence.
ACCESSION   DAXHNE010000001 DAXHNE010000000
VERSION     DAXHNE010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 49823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49823
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.001"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            135..1946
                     /gene="typA"
                     /locus_tag="ACFM4Z_000001"
     CDS             135..1946
                     /gene="typA"
                     /locus_tag="ACFM4Z_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFC6020124.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(2356..2446)
                     /locus_tag="ACFM4Z_000002"
     tRNA            complement(2356..2446)
                     /locus_tag="ACFM4Z_000002"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2410..2412),aa:Ser,seq:gga)
     gene            complement(2485..2571)
                     /locus_tag="ACFM4Z_000003"
     tRNA            complement(2485..2571)
                     /locus_tag="ACFM4Z_000003"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2535..2537),aa:Leu,seq:cag)
     gene            complement(2607..2693)
                     /locus_tag="ACFM4Z_000004"
     tRNA            complement(2607..2693)
                     /locus_tag="ACFM4Z_000004"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2657..2659),aa:Leu,seq:cag)
     gene            2782..5157
                     /gene="rnr"
                     /locus_tag="ACFM4Z_000005"
     CDS             2782..5157
                     /gene="rnr"
                     /locus_tag="ACFM4Z_000005"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFC6020125.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGMDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAAKKIPVHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKVGRNYRLVVAIADVSHYVRPDDVIDADAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPDVERLCMVCDMVVTYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWISDGIDHPYKAQIDTLYKLFKILQKKRFERGAVEFESVETQMIFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALVEQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAGGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAEKKTARGGKVRGRGASAAAESRKKAKKPVPIKVKKRKGKS"
     gene            complement(5496..6959)
                     /gene="guaB"
                     /locus_tag="ACFM4Z_000006"
     CDS             complement(5496..6959)
                     /gene="guaB"
                     /locus_tag="ACFM4Z_000006"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFC6020126.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVKWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(7106..9664)
                     /gene="glnD"
                     /locus_tag="ACFM4Z_000007"
     CDS             complement(7106..9664)
                     /gene="glnD"
                     /locus_tag="ACFM4Z_000007"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HFC6020127.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKANRVSVFFREYTAALKTLL
                     AALWAEYFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLIPSVKSGSVDELCESVRNDITGDTAFLEARFLFGNRQTADKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATNLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNAKRAEDRLLFNLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAVKTVKQLSGILTPMLRSRVSSAPMRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTKEESDLLAWLVENHLLMSTVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILFKSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAEISSHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSPDLKNPKIQSSLKQALLEQLT"
     gene            complement(9713..10033)
                     /locus_tag="ACFM4Z_000008"
     CDS             complement(9713..10033)
                     /locus_tag="ACFM4Z_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6020128.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQMPS
                     ESGI"
     gene            10049..10318
                     /locus_tag="ACFM4Z_000009"
     CDS             10049..10318
                     /locus_tag="ACFM4Z_000009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020129.1"
                     /translation="MVKVKHLLLRILLHIKAKKRGGGSHTQTHTHQEERGKIKTDWET
                     ANKQSRFHIGIIKQKPLSGIDSNQFRFYSQTEKEKRHIRLYGYAV"
     gene            complement(10358..10831)
                     /gene="bfr"
                     /locus_tag="ACFM4Z_000010"
     CDS             complement(10358..10831)
                     /gene="bfr"
                     /locus_tag="ACFM4Z_000010"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC6020130.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(10859..11323)
                     /gene="bfr"
                     /locus_tag="ACFM4Z_000011"
     CDS             complement(10859..11323)
                     /gene="bfr"
                     /locus_tag="ACFM4Z_000011"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC6020131.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVSRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            11899..12366
                     /locus_tag="ACFM4Z_000012"
     CDS             11899..12366
                     /locus_tag="ACFM4Z_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFC6020132.1"
                     /translation="MKIENIDIISPELFPQETFNETEAFGALVWLWAVSPIYQHAGVQ
                     EAAVNILPVLKNGQFALFSSNGHPVAYCTWAYFDEETEWQYLQSNDVLRHSENWCSGN
                     RMWLINWFAPFGDSRMMKRILVHLFPKREIRWLYHRGSEKGKRIMRFPALSKQ"
     gene            complement(12407..12763)
                     /locus_tag="ACFM4Z_000013"
     CDS             complement(12407..12763)
                     /locus_tag="ACFM4Z_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020133.1"
                     /translation="MKYIVSISLAMGLAACSFGGFKPPPDDSVYWKYSRIEQEYPAMM
                     SKNINLRIYSYEEYSKQIDDLFAKEKKDWKECGYDPIGGGGGSKADACMRKRGWYRVG
                     DDIYPENKKYEWPREN"
     gene            complement(12760..13098)
                     /locus_tag="ACFM4Z_000014"
     CDS             complement(12760..13098)
                     /locus_tag="ACFM4Z_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020134.1"
                     /translation="MKYIVSISLAMGLAACSFGGFKPNPWDAASFWELKNYANPYPGS
                     ASAALDQYPSKARRRQLKDMQECGYDPIDGGKSEADACLRKKGWCRKGFDPYPENKKY
                     EWPREEGKTK"
     gene            complement(13095..13457)
                     /locus_tag="ACFM4Z_000015"
     CDS             complement(13095..13457)
                     /locus_tag="ACFM4Z_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC6020135.1"
                     /translation="MKYIVSISLAMGLAACSFGGFKPPPDDSAFWRLTNYAKLYPGLT
                     SATLDQYPPEERRRQLHDSFAREKKDWKECGYDPIGGGGGSEADACMRKRGWYRVGND
                     IYPENKKYEWPREEGKTK"
     gene            complement(13454..20275)
                     /locus_tag="ACFM4Z_000016"
     CDS             complement(13454..20275)
                     /locus_tag="ACFM4Z_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFC6020136.1"
                     /translation="MNKRCYKVIFNKKRSCMMAVAENVHRDGKSMQDSEAASVRVTGA
                     ASVSSARAAFGFRMAAFSVMLALGVAAFSPAPASGIIADKSAPKNQQAVILQTANGLP
                     QVNIQTPSSQGVSVNRFKQFDVDEKGVILNNSRSNTQTQLGGWIQGNPHLARGEARVI
                     VNQIDSSNPSLLNGYIEVGGKRAEVVVANPSGIRVNGGGLINAASVTLTSGVPVLNNG
                     NLTGFDVSSGKVVIGGKGLDTSDADYTRILSRAAEINAGVWGKDVKVVSGKNKLDFDG
                     SLAKTASAPSSSDSVTPTVAIDTATLGGMYADKITLISTDNGAVIRNKGRIFAATGGV
                     TLSADGKLSNSGSIDAAEITISAQTVDNRQGFIRSGKGSVLKVSDGINNQAGLIGSAG
                     LLDIRDTGKSSLHINNTDGTIIAGKDVSLQAKSLDNDGILTAARDVSVSLHDDFAGKR
                     DIEAGRTLTFSTQGRLKNTRIIQAGDTVSLTAAQIDNTVSGKIQSGNRTGLNGKNGIT
                     NRGLINSNGITLLQTEAKSDNAGTGRIYGSRVAVEADTLLNREETVNGETKAAVIAAR
                     ERLDIGAREIENREAALLSSSGDLHIGSALNGSRQVQGANTSLHNRSAAIESSGNIRI
                     ATKDLQNTNEHLRFHTEETHREHRIEYQAEGRTERYPEGSQKELGWEIFEDESLHMRT
                     PDGSPHSVWYKYDYERITAESKITESKPGQIISGGNLVLDAAKLKNHNSRIIAGGRLI
                     VGTPESALDNDETLGTKTITDKGDLHRYHRHHKKGRDSTGYSRSPYEPAPEVSSIRMG
                     ISAYKGYAPQQASDIPGTVVPVVAENGIHPTFTLPNSSLFAIAPNNKGYLIETDPAFT
                     DYRKWLGSGYMLAALQQDPNHIHKRLGDGYYEQKLVNEQIAKLTGYRRLDGYTNDEEQ
                     FKALMDNGITIAKELQLTPGIALSAEQVARLTSDIVWLENETVTLPDGTTQTVLKPKV
                     YVRARPKDMNGQGALLSGSVVDIGSGAIENRGGLIAGREALILNAQNIKNLQGDLQGK
                     NIFAAAGSDITNTGSIGAENALLLKASNNIESRSETRSNQNEQGSVRNIGRVAGIYLT
                     GRQNGSVLLDAGNNIVLTASELTNQSEDGQTVLNAGGDIRSDTTGISRNQNTIFDSDN
                     YVIRKEQNEVGSTIRTRGNLSLNAKGDIRIRAAEVGSEQGRLKLAAGRDIKVEAGKAH
                     TETEDALKYTGRSGGGIKQKMTRHLKNQNGQAVSGTLDGKEIILVSGRDITVTGSNII
                     ADNHTILSAKNNIVLKAAETRSRSAEMNKKEKSGLMGSGGIGFTAGSKKDTQTNRSET
                     VSHTESVVGSLNGNTLISAGKHYTQTGSTISSPQGDVGISSGKISIDAAQNRYSQESK
                     QVYEQKGVTVAISVPVVNTVMGAVDAVKAVQTVGKSKNSRVNAMAAANALNKGVDSGV
                     ALYNAARNPKKAAGQGISVSVTYGEQKNTSESRIKGTQVQEGKITGGGKVSLTASGAG
                     KDSRITITGSDVYGGKGTRLKAENAVQIEAARQTHQERSENKSAGFNAGVAIAINKGI
                     SFGFTAGANYGKGYGNGDETAYRNSHIGSKDSQTAIESGGDTVIKGGQLKGKGVGVTA
                     ESLHIESLQDTAVFKGKQENVSAQVTVGYGFSVGGSYNRSKSSSDYASVNEQSGIFAG
                     GDGYRIRVNGKTGLVGAAVVSDADKSKNLLKTSEIWHKDIQNHASAAASALGLSGGFS
                     YSPKPTSGQYSTKKEAEIGKIGGKPVSLMRFDQVSAKDDELNEKYRSERIEKGETFKE
                     ANLNQNNAGGLKFGLKQNDIHSNDKYALAKMGLGNLLGNAKESESRQSITRSVISEGD
                     WQIASAQGRKNIAGIEKGTSSAHKALAKADREGLLKEVELNRDVAKEFINETLIGGIA
                     DEAYRSQFIAEHRLMTFKMDENGEPIEDKQLEEDINKQFDNSVKLKKEFASFKDYWEA
                     YKAIGGNIYELREVSDQERKNLKTARYTDPETGKTVEKIVVGVNGIFNNIQAAAKFAA
                     QQYVGRFNPEKNRYERTYENVYFLHNPETNGRGFSKLPEIAVAAFHKMLEGAKIGNKT
                     VIGLSNSGLALGNIMEDYGKDKNGLFVGSHSRGTLVVDNVLNTLNTQANRDKKILSNT
                     ELKMVGPAANVVRADKRLFQLQQGVTTPRTADFARQSIQIENHELDLIGMLIGRNPAT
                     VGTNTRQKSQWQAIRDIIGDYTSPHNCYGMANKQCVTDGYRDPENKQTQSPTGVFERG
                     VSNEIEIMYRPVRIYDLQHPKGKTK"
     gene            complement(20785..21768)
                     /gene="lipA"
                     /locus_tag="ACFM4Z_000017"
     CDS             complement(20785..21768)
                     /gene="lipA"
                     /locus_tag="ACFM4Z_000017"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFC6020137.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPRNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(21761..22384)
                     /gene="lipB"
                     /locus_tag="ACFM4Z_000018"
     CDS             complement(21761..22384)
                     /gene="lipB"
                     /locus_tag="ACFM4Z_000018"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFC6020138.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(22386..22661)
                     /locus_tag="ACFM4Z_000019"
     CDS             complement(22386..22661)
                     /locus_tag="ACFM4Z_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HFC6020139.1"
                     /translation="MTEQKNKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            22846..23916
                     /locus_tag="ACFM4Z_000020"
     CDS             22846..23916
                     /locus_tag="ACFM4Z_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFC6020140.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMLAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            complement(24113..25060)
                     /locus_tag="ACFM4Z_000021"
     CDS             complement(24113..25060)
                     /locus_tag="ACFM4Z_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HFC6020141.1"
                     /translation="MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(25075..25482)
                     /locus_tag="ACFM4Z_000022"
     CDS             complement(25075..25482)
                     /locus_tag="ACFM4Z_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFC6020142.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVRKVETDSYQDLDAGQYVEILRHTGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIITHP"
     gene            25563..26222
                     /gene="ung"
                     /locus_tag="ACFM4Z_000023"
     CDS             25563..26222
                     /gene="ung"
                     /locus_tag="ACFM4Z_000023"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFC6020143.1"
                     /translation="MDTWHDALGNEKHQPYFQKILNAVRQERLSGQIIYPPETDVFNA
                     FRLTAFDRVKVVILGQDPYHGAGQAHGLAFSVRQGVRIPPSLLNIYKELETDINGFSI
                     PDHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLAAHRKHLVFML
                     WGGYAQQKRKLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            26370..26972
                     /locus_tag="ACFM4Z_000024"
     CDS             26370..26972
                     /locus_tag="ACFM4Z_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFC6020144.1"
                     /translation="MRAFIKNYVLTLLFLKYVSDKHKYGGGMIELHAGTTFDDIVKLK
                     NTADIGDRLNKIIAQIAEANDLKGVIDVTDFNDEDKLGKGKEMIDRLSRLVGIFKKLN
                     LSSNQAEDDDLLGDAYEYLMRHFATESGKSKGQFYTPAEVSRIMAKIIGISADCRPAP
                     AFMTRPAARVRCCSKPPPKPAAKSAFTVRKKMWQPRPLPV"
     gene            26885..27518
                     /locus_tag="ACFM4Z_000025"
                     /pseudo
     CDS             26885..27518
                     /locus_tag="ACFM4Z_000025"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            27506..27820
                     /locus_tag="ACFM4Z_000026"
     CDS             27506..27820
                     /locus_tag="ACFM4Z_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HFC6020145.1"
                     /translation="MVHLSEIAAQDYNLNLPRYIDSGEVEDLQNLAAYLYGGIPAHDM
                     DALEAYWQVLGRMKNELFAEHDGRFTTIQRNRLQIFPTLNSLKSCGIGVEFRANLVQP
                     QI"
     gene            27862..28806
                     /locus_tag="ACFM4Z_000027"
     CDS             27862..28806
                     /locus_tag="ACFM4Z_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFC6020146.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAAIQPGRLIRKWSESLLD
                     AFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKESDEDANLTV
                     VFEETETGKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLKEKQSELERLSQEL
                     ENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQTLMTQEKA
                     AKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLENSIQQLIS
                     RLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            28986..29081
                     /locus_tag="ACFM4Z_000028"
     CDS             28986..29081
                     /locus_tag="ACFM4Z_000028"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020147.1"
                     /translation="MAHMDNFLPNWQSIKQQLNALELFAQIYNLT"
     gene            complement(29371..29886)
                     /locus_tag="ACFM4Z_000029"
     CDS             complement(29371..29886)
                     /locus_tag="ACFM4Z_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020148.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYIPPQTPEPVSSPSNGGQVVKYKAPVKTVSKPAKSNTPPPQQ
                     APSNNSRRSILETELSNERKALVEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(30352..32271)
                     /locus_tag="ACFM4Z_000030"
     CDS             complement(30352..32271)
                     /locus_tag="ACFM4Z_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6020149.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERIAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIIRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIIVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRAE
                     KEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSLLAKVKTQLAQIEEVWLACQEE
                     LERIETEIEKQFAER"
     gene            complement(32348..32719)
                     /locus_tag="ACFM4Z_000031"
     CDS             complement(32348..32719)
                     /locus_tag="ACFM4Z_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HFC6020150.1"
                     /translation="MLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVHKDSVCLSQ
                     CRTLSDLTPENLLSDVKPVDYPEILIIGTGAAQEFIHPKIIADFSRIGISVECMNTDS
                     AFRTLVFLHSEGRRAWAWLQP"
     gene            32916..34223
                     /locus_tag="ACFM4Z_000032"
     CDS             32916..34223
                     /locus_tag="ACFM4Z_000032"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFC6020151.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQTCPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            34216..34650
                     /locus_tag="ACFM4Z_000033"
     CDS             34216..34650
                     /locus_tag="ACFM4Z_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020152.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(34709..34978)
                     /locus_tag="ACFM4Z_000034"
     CDS             complement(34709..34978)
                     /locus_tag="ACFM4Z_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFC6020153.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(35160..37610)
                     /gene="lon"
                     /locus_tag="ACFM4Z_000035"
     CDS             complement(35160..37610)
                     /gene="lon"
                     /locus_tag="ACFM4Z_000035"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFC6020154.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAALEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIETVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDTDHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DSKTKKTGKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVGGELL
                     TVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIHVHVP
                     EGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKLLAAL
                     RGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEPSGAE
                     AAAESASKPKPRSRATKH"
     gene            37710..37886
                     /locus_tag="ACFM4Z_000036"
     CDS             37710..37886
                     /locus_tag="ACFM4Z_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase"
                     /protein_id="HFC6020155.1"
                     /translation="MPSETFRLQTAWAWKDKAGTKNCSVLPLLAVPSVWFAVLPPYFQ
                     NMTQVFHVFFYFSR"
     gene            37861..38964
                     /locus_tag="ACFM4Z_000037"
     CDS             37861..38964
                     /locus_tag="ACFM4Z_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC6020156.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRSALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLHLRPSDFLDKAFAQAGLRVADMM
                     KTKPKHKKAANPSDPLAFARTRETTFLYRLKKA"
     gene            complement(38992..40737)
                     /gene="recD"
                     /locus_tag="ACFM4Z_000038"
     CDS             complement(38992..40737)
                     /gene="recD"
                     /locus_tag="ACFM4Z_000038"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFC6020157.1"
                     /translation="MERQTDEFAQAAARAAIRFLEHYAGSGDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFNPVYPLPFDVLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCRHVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEEAFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(40805..41500)
                     /gene="lolD"
                     /locus_tag="ACFM4Z_000039"
     CDS             complement(40805..41500)
                     /gene="lolD"
                     /locus_tag="ACFM4Z_000039"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFC6020158.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEIHAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAEAAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            complement(41493..42740)
                     /locus_tag="ACFM4Z_000040"
     CDS             complement(41493..42740)
                     /locus_tag="ACFM4Z_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HFC6020159.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDTVWVRDWTFSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSRRASKTQPAEALRYE"
     gene            42945..43223
                     /locus_tag="ACFM4Z_000041"
     CDS             42945..43223
                     /locus_tag="ACFM4Z_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020160.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(43279..43896)
                     /gene="recR"
                     /locus_tag="ACFM4Z_000042"
     CDS             complement(43279..43896)
                     /gene="recR"
                     /locus_tag="ACFM4Z_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFC6020161.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(43963..45501)
                     /locus_tag="ACFM4Z_000043"
     CDS             complement(43963..45501)
                     /locus_tag="ACFM4Z_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HFC6020162.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISEQSLNAAVQNEQADGGSPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(45581..45934)
                     /locus_tag="ACFM4Z_000044"
     CDS             complement(45581..45934)
                     /locus_tag="ACFM4Z_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFC6020163.1"
                     /translation="MIVLHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQRPSEAEISL
                     WLEQVPLEILLNKRGTTWRKLNALMQEEALSSKEGAVRIMSEMPSLIKRPVLEYGGKV
                     HVGFSDESYREIFKR"
     gene            46084..47712
                     /locus_tag="ACFM4Z_000045"
     CDS             46084..47712
                     /locus_tag="ACFM4Z_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFC6020164.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQDGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            47784..49037
                     /locus_tag="ACFM4Z_000046"
     CDS             47784..49037
                     /locus_tag="ACFM4Z_000046"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HFC6020165.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYAGFSFHTDKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYKFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVEKLRSKTIIDVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
     gene            49122..49799
                     /locus_tag="ACFM4Z_000047"
     CDS             49122..49799
                     /locus_tag="ACFM4Z_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISNme3 family transposase"
                     /protein_id="HFC6020166.1"
                     /translation="MKLKNKYQKFSKISEQKFREIIRCFALDLTASDTAKMTGISVRG
                     INPIFLKIRHRIAALCEQSSPLSGVVELDESYFGARRIRGKRGRGASGKTIVFGILKR
                     NGVVYTEIVPDASKASLIKVIRGHISADSEINTDGWKAYDGLVDMGYEKHYRVHHGSN
                     EFARGKQHINGIESFWSYTKGRLAKFHGISKEMFYLHLKETEFRFNHRHEDLGKILMK
                     MLRNNPI"
BASE COUNT        11240 a        12765 c        13164 g        12654 t
ORIGIN      
        1 tcctgatttt tgttaatcca ctataccgtc tgaaaggcaa gggcttcaga cggcatcttc
       61 atttcccaaa tactgtccgg taaagcgtgg tataatgcgt agttatctct tacagttttt
      121 gaaaaacatt aattatgaaa caaatccgca acatcgccat catcgcccac gtcgaccacg
      181 gcaaaaccac attggtcgac caactgctgc gccaatccgg cacattccgc gccaaccagc
      241 aggttgacga gcgcgtgatg gacagcaacg accttgaaaa agaacgcggc atcaccatcc
      301 tcgccaaaaa caccgccatc gattacgaag gctaccacat caatatcgtc gacacgccgg
      361 gacacgccga cttcggcggc gaagtagagc gcgttttggg gatggtggac tgcgtcgtct
      421 tgttggtgga cgcgcaggaa ggcccgatgc cgcaaacccg tttcgtgacc aaaaaagcct
      481 tggctttggg gctgaaaccg attgtcgtca tcaacaaaat cgacaagccg tccgctcgtc
      541 cgagctgggt tatcgaccaa actttcgagc tgttcgacaa cttgggcgcg accgacgagc
      601 agttggattt cccgattgtt tacgcttcag ggttgagcgg tttcgccaaa ttggaagaaa
      661 ccgacgagag caacgacatg cgtccgctgt tcgatactat cttaaaatat acgcctgcac
      721 cgagcggcag cgcggacgaa acgctgcaac tgcaaatttc ccaactcgac tacgacaact
      781 acaccggccg cctcggtatc ggtcgtatct tgaacggacg catcaaaccc ggccaaaccg
      841 ttgccgtcat gaaccacgat cagcaaatcg cccaaggccg catcaaccag cttttgggtt
      901 tcaaaggttt ggaacgcgtg ccgcttgaag aagccgaagc cggcgacatc gtgattattt
      961 ccggtatcga agacatcggt atcggcgtaa ccatcaccga caaagacaat cccaaaggcc
     1021 taccgatgtt gagcgtggac gaaccgacgc tgacgatgga ctttatggtc aacaccagcc
     1081 cgctggcggg tacggaaggc aaattcgtaa ccagccgcca aatccgcgac cgcctgcaaa
     1141 aagaattgct gaccaacgtc gccctgcgcg tggaagatac cgccgatgcc gacgtgttcc
     1201 gcgtatccgg gcgcggcgag ctgcacctga ccattttgct ggaaaacatg cgccgcgaag
     1261 gctacgaact cgccgtcggc aaaccgcgcg tcgtgtaccg cgacatcgac ggtcaaaaat
     1321 gcgaaccgta tgaaaacctg accgtggatg tacccgacga caaccaaggc gcggtaatgg
     1381 aagaactcgg ccgccgccgt ggcgaactga ctaatatgga aagcgacggc aacggacgca
     1441 cccgcctcga ataccatatt ccagcgcgcg gcttgatcgg tttccaaggc gaatttatga
     1501 ccctgacgcg cggggtcggg ctgatgagcc acgtgttcga cgattacgcg cccgtcaaac
     1561 ccgatatgcc cggccgccac aacggcgtgc tggtgtccca agagcagggc gaggcagtcg
     1621 cttacgcctt gtggaatctg gaagaccgcg gccgtatgtt cgtatcgccc aacgacaaaa
     1681 tctacgaagg catgattatc ggcatccaca gtcgcgacaa cgatttggtg gtcaacccgc
     1741 tcaaaggcaa aaaacttacc aacatccgtg ccagcggtac cgacgaagcc gttcgcctga
     1801 ccacgccaat caagctgacg ctggaaggtg cggttgagtt tatcgacgat gacgaactcg
     1861 ttgaaatcac gccgcaatcc atccgtctgc gcaagcgtta cttgagcgaa ttggaacgcc
     1921 gccgccactt taaaaagctg gattgatgtt tactggatta atgtttaaat gatacgcgat
     1981 gccgtctgaa aaatttcaga cggcattttt tattcggacg ggctttgcgg cttcttgaag
     2041 ctgtttcaga cggcgttttt cctacccaat caagaaactg ccgccatttt tccagcggta
     2101 tatcgccccg tcgcgtttcg gtatcgggtt cggcttcccg gcagcatgtc gaacaatgcc
     2161 gtttgaagga atacgccttt tgagtcctta caggctgagg aagagggtaa aacataccgc
     2221 aaaacatacc acaaaaaacg gtaacggata gttgtaagcg gttgatgacg attatagaca
     2281 ataacgggtt ttcccaatga aattattgtt tgaataataa aaaatcccaa accgtaaaag
     2341 tttgggattt gtatttggca gagaggaagg gattcgaacc ctcgatacgc tattcacgta
     2401 tacacgcttt ccaggcgtgc gacttaaacc actcatccac ctctctaatg gcggaaatta
     2461 tcccaatcgg gataatttat tatttggtgc ccgggagaag actcgaactt ccacacccat
     2521 gaggatacca gcacctgaag ctggcgcgtc taccaattcc gccacccggg caatctaaat
     2581 attgaaataa agcaaagcat ttgatttggt gcccgggaga agactcgaac ttccacaccc
     2641 gtgaggatac tagcacctga agctagcgcg tctaccaatt ccgccacccg ggctttgctt
     2701 tatttgctgt tttgcggtac agtgtcctgc cgcaaagaag tcgctattat atagttcata
     2761 aagagaatgt caacagtcca aatgaataaa aatattaaat ctttaaattt acgggaaaaa
     2821 gacccgtttt taagtcgtga aaaacagcgt tatgaacatc ctttgcccag tcgggaatgg
     2881 ataatcgaat tgttggagcg caaaggtgtg ccttcaaaaa tcgaatcgct tgcgcgcgag
     2941 ctgtcgatta cggaagacga gtatgtcttt tttgaacgcc gtctgaaggc gatggcgcgg
     3001 gacggtcagg ttttaatcaa ccgtcggggc gcggtttgcg cggcggacaa attggatttg
     3061 gtcaaatgcc gcgtcgaggc gcataaggac ggtttcggtt ttgccgtgcc gctcacgccc
     3121 gccaaagacg gtgattttgt tttgtatgaa cgccagatgc gcggcattat gcacggcgat
     3181 attgtcactg ttcgtcctgc cggcatggac cgtaggggcc gccgcgaagg gacagttctg
     3241 gatattgtcg aacgcgcgca aagcaaagtg gtcggccgtt tctatatgga taggggcgtg
     3301 gcgattttgg agccggaaga caagcgtctg aaccaaagca tcgtattgga accggacggc
     3361 gtggcgcgtt tcaaacctga atccggtcag gtcatcgtcg gcgaaattga ggtttatcct
     3421 gagcaaaacc ggccggcagt ggcaaaaatc atcgaagttt tgggcgatta tgccgacagc
     3481 ggcatggaga ttgaaattgc cgtgcgcaag catcatttgc cgcaccaatt cagtgaagcg
     3541 tgtgccaaag ctgcgaaaaa aattcccgtc catgtacgca aaagcgattt gaaaggccgc
     3601 gtcgatttgc gcgacctgcc tttggtaacg atagacggcg aaacggcgcg cgatttcgac
     3661 gacgcggtgt ttgccgaaaa agtcggacgc aattaccgtc tggtcgtggc gattgcggat
     3721 gtcagccatt atgtccgccc tgacgatgtg attgatgcag atgctcaaga acgcagtacc
     3781 agcgtatatt tcccgcgccg tgtgattccg atgctgccgg aaaacctgtc taacggcatt
     3841 tgctcgctca atcccgatgt cgagcgtttg tgtatggtgt gcgatatggt cgttacctat
     3901 gcgggcaata tcaaagaata ccgcttctac cccgccgtaa tgcgctctca tgcccgcctg
     3961 acctacaacc aagtttggaa atggatttca gacggcatcg accatccgta caaagcccaa
     4021 atcgacaccc tttacaaact cttcaaaatc cttcagaaaa agcgtttcga acgcggcgcg
     4081 gtggagtttg aaagcgtcga aacccagatg attttcgatg acaacggcaa aatcgaaaaa
     4141 atcgtccccg ttgtccgcaa cgatgcccac aagctgattg aagaatgtat gctggcggcg
     4201 aatgtttgcg cagcggattt cctgttgaaa aacaagcata cggctttgtt ccgcaaccat
     4261 ttgggcccca cgcccgaaaa actcgccacc ctgcgcgagc agctcggtct gttggggctt
     4321 caacttggcg gcggcgacaa cccgtcgccg aaagactatg ccgcgcttgt cgaacaattc
     4381 aaaggcagac ctgatgccga attgctgcaa gtcatgatgt tgcgctccat gcagcaggcg
     4441 gtttacgaac cgcattgcga cggacacttt ggtcttgcct acgaagcata cgcccacttc
     4501 acctcgccca tccgccgcta tcccgacctg accgtacacc gcgccatcaa agccgtgttg
     4561 aatcagcaaa cctacacgcc aaaaaaaagc tggcaggctt tgggcgtgca tacctcgttc
     4621 tgtgagcgcc gtgccgacga cgccagccgc gacgtggaaa actggctgaa aacctattat
     4681 atgcgcgata aggtcggcga agtattcgaa ggtaaaatct ccggcatgac cagttttggt
     4741 atctttgtaa cactggacgg catccacatt gacggcttgg tgcatatcag cgatttgggc
     4801 gaagactatt tcaacttccg ccccgaaatc atggcaatcg aaggcgaacg cagcggcatc
     4861 cgtttcaaca tgggggacag ggttgccgtc cgggtcgccc gtgccgattt ggatgacgga
     4921 aaaatcgatt ttgtcctgat tgccgggggg agcggcaggg ggcggaaagt taaatcatcc
     4981 gcgtctgcca aaccggcagg gacggcgggg aaagggaagc cgaaaaccgc cgccgagaaa
     5041 aaaacagccc gaggcggcaa agtaagggga aggggcgcgt ctgccgccgc agaatcgagg
     5101 aaaaaggcaa agaaaccggt tccgattaag gtaaaaaaac ggaaaggcaa atcataatgc
     5161 tgacggggcg gcttggggag ggcggggcat actttcccga agccgccgcc cgattttttg
     5221 atatggcggc cggcgcatca tttcaggcca atcgaaacgc ccgtattgaa agattgcgtt
     5281 tatttccacc gccgttttaa aggccggcgg tattcggcag acggggcgca aacggcgttc
     5341 agacggcatt ttcattcttt cggcgtgtcc gtccgaatgg ctttgcccgt ccgcgcaatc
     5401 agcccgtgcg gcttgccttg aacggacaaa aaatgccgtc tgaaacccga aaatcaggtt
     5461 tcagacggca ttttgccttg aaaaggctgt tcaaatcagc ggtggtagtt cggtgcttct
     5521 ttggtaattt gaacgtcgtg aacgtgcgat tcgctcatac ctgcggaagt gatttccaca
     5581 aattctgctt tttcgtgcat ttcggcaata ttggcgcaac ccaaataccc catgctggag
     5641 cgcagtccgc cggtcagttg gtggatgatg ttcacaatcg ggcctttgta gggaacgcgg
     5701 ccttcgatgc cttcggggac gtatttgtcg gtgctgtcgg ttttgtcttg gaagtagcgg
     5761 tcggcagaac cttggctcat cgcgcccaat gaacccatac cgcgatagga tttgtacgag
     5821 cggccttggt agagttcgat ttcgcccggc gcttcttccg tgcctgcaaa cataccgccg
     5881 agcatgacgc tgtacgcgcc tgcggcgagg gctttggcga tgtcgccgga gaagcggatg
     5941 ccgccgtcgg caatcagcgg aacgcccgtg cctttgaggg cttcggcaac gttgtgaatg
     6001 gcggtcagtt gcggcacgcc gacacctgcc acgatacggg tggtgcaaat cgatcccgga
     6061 ccgataccga ctttgacggc atccgcgccg gcggcgacca aatccaatgc ggctttggca
     6121 gtggcgatgt tgccgccgat gacttggatg tgcggatagg tttctttcac ccatttcacg
     6181 cggtcgatca cgccttggct gtgcccgtgg gcggtatcga cgacgatgac atcgactcct
     6241 gcttctacca atgcttttac gcgctcttcg gtgtcgccgc cggtgccgac tgccgcaccg
     6301 acgcgcagac ggccctcgga gtctttgttg gcattgggaa attcggtggt ttttaaaata
     6361 tctttgacgg taatcagacc tttgagttcg tctttttcgt tcagaaccaa aacgcgctcg
     6421 actttgtgcg tgtgcatcag ttcgcgcgct tcgtctatgc ttgtgccttc ggggacggta
     6481 accagacgtt cgcgcggggt cataatggcg gaaacgggca aatcgacgcg gttttcaaaa
     6541 cgcaggtcgc ggttggttac gatgccgacg actttgccgt tttcaacgac gggcaggccg
     6601 gacattttgc gtttgcgctg cgcgcgcatt tccaagactt cgcggatgag cgtcgtcggc
     6661 gcaacggtta cggggtcttt gaccacgccg ctttcgtggc gtttcacttt ggaaacggcg
     6721 cgcgcctgca tctccggcgg catattttta tggatgatgc cgatgccgcc ttcctgtgcc
     6781 atcgaaatgg cgaggcgcgc ctcggtaaca gtatccatcg cggcggaaag cagggggagg
     6841 ttgagcgtga tttcgcgggt gagcttggtt tgaagtttaa cgtctcgcgg cagcacggtc
     6901 gaatgtgcgg gaaccaacaa aacatcgtcg aaagtatagg ctttttctac gatacgcata
     6961 atgctcggtc tttcagtttg tgcaagatgc acggcattat agcacgttac cggcggcttg
     7021 acagtttata ttgtttgcaa tatcccccgc cttcagacag cgtacggcgc ggcggcggga
     7081 taccgtctga aaaccggcag ggtgcttatg tcaactgttc cagcaaagcc tgcttcaatg
     7141 aggactggat ttttgggttt ttcaggtcgg ggctaaaaac ggtaaaactg tcttcggcgc
     7201 gttcatccag tgtggagatt ttggcatagc gcaggctgac gttgtgggcg aaaaagactt
     7261 ccgccatatc ggcgagcagg aaggggcggt tgacggcggt aatttcgacg gagtaccagt
     7321 ccggatagtc ttcttcgggg gtgatggtga tgcttggcgc gatcggcatg tagcggctgc
     7381 ggcggctgat gcggcggcta tggctggaga tttcggcaac ggtgtgtccg tggataaagc
     7441 tgttgagttc ggcttcgagc gcgctttgga tgtcggggta gtcttcgggg gcgtgctgcg
     7501 agggaatttg cacgatgaag gtgtcgagga tgtagtcgtg ttcggtgata aaggcgcggg
     7561 cggcgagaat gtcgaagccg tggcggctga agatacggca gagacgggca aacaagcgcg
     7621 gcccgttggg catgaaaacc ataacttgaa agctgtcgct tttgaacagg atgcggctgc
     7681 ggacgatggg ggtttcaaaa tcgtggacta ggttggcggc gtgccacagg atttcgcggg
     7741 actggtggcg ggcgaagtag gcggaaccga gcgcgttcca tagttttttc tgctgttttt
     7801 cggggacggc ggcgcgggtg agtaagtcgg cggcttcctg ccggcggcgg ccgaagaggg
     7861 tgtgcgggtt gccgccgttg cctgtaaggt agcgtccggc ggcatggaag aggctttcca
     7921 gcaggctggc gcgccaggcg ttccatagtt tgggattggt gccgcgtatg tcggaaatgg
     7981 tcaggaggta gagtgcgctg aggcgttcgt gggtttgcac gcgtttgcag aaggcatcga
     8041 gtacgtcggg gtcttggatg tcttcttttt gggcgacggt ggacataaga agatgatttt
     8101 cgaccagcca ggcgagcagg tcgctttctt ctttggtcag gaagtggtcg gcggcaaatt
     8161 ggcgtgcgtc cgcgatgcct tgtatggcat ggtcgccgcc gcgtcctttg gcgatgtcat
     8221 ggaaaaaggc ggcaaggtag aggatgtctt gtttttcaaa ggactgcatc agtgcagagg
     8281 cgtagggcag ctcgtggctg tgcatatcca aggcaaggcg gcggacgttg cggacgacgg
     8341 tgaggatgtg gtcgtccacg ggatagatgt ggaacaggtc gtgctggagc aggccgacga
     8401 ttttttccca cgcgggcagg tagcgtccca atacgccata gaggttgagg aagcgcaggg
     8461 tttgggtcag tccgttgccg ttgcggaaaa agccggcgaa gcggtggcgg ttttcagaat
     8521 tttggtagaa gctgcggttg attttgcgcg tcgcgcccca ccatgcgcgc agggtttgcg
     8581 gttcgagcgc ggtaatatcg ttgcgctgct gcatgatttc gacgattttg aaaatgtgtt
     8641 cgggccgtct gaaaaaaata tcggtgtgcc gcgcggcgat ttggttgttg acttggatgt
     8701 agtcgtcgtc aatccgcagg gtaacgcgca tcggcgcgga ggaaacgcgg cttcgcagca
     8761 taggcgtgag gatgccgctc agttgtttga cggttttgac cgcacggtaa aacacgcgca
     8821 tcagttcttc gctttggcgg cggaggttca agccttcata acccatgctt tcggcgactt
     8881 gcggctgcaa attgaacagc aggcggtctt cggcgcgctt ggcgtttaaa tgcagatgga
     8941 tgcggatgtg ggcgaggcgg cggtagccgt gcgaaagcat accggcttcg gcacgcgtca
     9001 aaatccgctg tttgagcagg tcgggcaggt tggtcgccaa gccttgtgct ttcgctatcc
     9061 aaagcagggt gtggatgtcg cgcagaccgc ccggacagct tttgatgttt ggttccaata
     9121 ccgcccctga gccttgcgat ttggcgtggc ggtgttccat ctccaccagt tttgcctcga
     9181 caaacgccgc cacattgcgc tgcgcgttca ttttttccgc tagtttatcc gccgtttggc
     9241 ggttgccaaa caaaaaccta gcctctaaaa acgctgtgtc ccccgtaata tcattgcgca
     9301 cgctttcaca tagttcatca acgctgccgc tttttacaga cggtatcagt ttgcagtccc
     9361 acagggtttg gacaaaccgg gcaatctgtt cctgaatgcc gtctgaaagc ggggcagggg
     9421 agacaaccgc caaatccaca tccgaacagg gatacagttc gccgcgtccg aagccgccta
     9481 ccgccatcag gcataacgcg ctgttttgaa aatactctgc ccacaatgcc gccaacaagg
     9541 ttttgagtgc cgccgtatat tccctgaaaa ataccgacac gcggttggct ttcaaataat
     9601 gcgcttcggc ggcatcgcgc tgctgtttga aggtttccag tgctgaagac aggtttgcag
     9661 gcatttttat tctttcgatt ggcgggaaaa ggggaggcgg atggttcggc ggtcaaatac
     9721 cgctttcaga cggcatttgt cgggtatcgg cggaattggt catcaggttc aaccgttcct
     9781 gcggcggcag cagcaactga tacgccgcca cgcccaaagc cgccgccatt ttttcgatat
     9841 tcgacagggc gatgttccag cgtttgcgtt cgactgccga cacataagtc ctgtccaaac
     9901 cgcattgccg cgccaattct tcttgcgacc aacccttgtt cacgcggaaa agccgcatat
     9961 tgtatgccaa taccgcccgc aaatcctgtt cgtcaggcag ttcggcaggc agagtcaatt
    10021 tgttgcccat cattatgttt ccggataaat ggttaaagtt aagcatttgc tgttacggat
    10081 tttacttcac ataaaagcca aaaaaagggg tggcggcagc cacacccaaa cacacacaca
    10141 tcaagaggaa agaggtaaaa tcaagacaga ttgggaaaca gcaaacaagc aatctagatt
    10201 tcatatcggt attatcaaac aaaaaccctt atcaggtatt gattcaaatc aatttagatt
    10261 ttattcgcaa accgaaaaag aaaaacggca tatccgttta tacggatatg ccgtctgatg
    10321 gtgcggggga ctgctgtatt tggcagtagt ttgtgtttta gtcctcttgc gccgctgttt
    10381 gcaggtagtt gggcagaccg attttgccga taagctcttg ctgggtctcc agccagtcta
    10441 tatgttcttc gttggtgtct ttttgttttt ccaacaaatc gcggctgacg taatcctgtt
    10501 gcgcttctgc tgtggcgatg gcggcaagca gggcttcgtg tttttcctgt tctttggtca
    10561 aatcgcaggc gatgatttct tcggtggact cgccaatcag aagcttgccc agttcttgca
    10621 ggttcggcag accttcgagg aacagaatgc gctcgatcaa atcgtcggca gctttcattt
    10681 caacgatgga ctgtttgaag aaatgttcgc ccagttcttc aaagccccag tttttcaaaa
    10741 tacgggcgtg aaggaaatat tggttgatgg ttaccagcag caagcctaag tttttgttca
    10801 gctcgcggat aaccaaacgg tcgcctttca tacggactcc ttttattccg aatacggatt
    10861 acagttggct ttggtagtag ttgccttcgc caatcagctc gatcaggcgc agctgctgtt
    10921 ccagccagtg ggcgtggtct tcttcggtat ctttcagttg ggcgaccatc aaatcgcggc
    10981 tgacatagtc ttgcgcctct tcgcacagtt taatgccttt tttcaaagcg tcgcgcactt
    11041 cgtattcggt ttgcaggtcg gctttgaggc aggaaaccac gtccgtgccg atattcagct
    11101 cggcgcgcgc cattttcggc gtaccgccca gcatcaggat gcgtcggatg aagtcttcgg
    11161 cgtgtgtggt ttcttcttcc atctcgtgat tgagacgttc aaaaagtttg gtgtagcccc
    11221 attcggagta gaggcgggag tggataaagt attggtcgcg tgccgccagc tcgccagaca
    11281 gcaattcgtt catataatca acaacagctt gattgccttg cataatatct ctctttccgt
    11341 aacttggttt tcggtatgtc tgaacagcat ctgtgtcgct attcgggatg cgtgcattgt
    11401 atgcaaaagc tgtcggcatg acaaattttc tatttaaaat acaaacaatt atcaaaagaa
    11461 atagggcgta ttccccgatg ctttccaaat cagtatgctg tttcttatcg gtttttttgc
    11521 tgctaaaact aagaatccat ctcatccata aagaacattt acacttatta accatagcaa
    11581 aaaacaaata gagcacggtt ttttatcaaa atttataatg aatcgttctc attaaccgac
    11641 agattctttt gagattatcg gattttggga ataaattatg ccgtctgagg ggctttcaga
    11701 cggcatgcct gcccggacag acgctgcttg gagcggctgt cggtttgaag gtgtgtttaa
    11761 tgcgaagggc aatctgtcgt atgatgtgtt tgcggcaggg gctttgaaga agcccggtat
    11821 tttaggacga agcgcgcggc tgccggggtc aatatccgct atttattcca aaccttatta
    11881 aaacttaaat ttaaaatcat gaagatagaa aatatcgata ttatttcgcc ggaactgttc
    11941 ccgcaggaaa cattcaatga aaccgaagca ttcggcgctt tggtatggtt gtgggcagtt
    12001 tcgcctattt atcagcatgc cggcgtacaa gaagccgccg tcaatatctt gccggtattg
    12061 aaaaacgggc aatttgcctt gttcagcagc aacggacacc ccgtcgccta ctgcacttgg
    12121 gcttatttcg atgaagaaac cgaatggcaa tatctccaat ccaatgatgt tctgcgccac
    12181 tcggaaaact ggtgcagtgg aaacagaatg tggcttatca actggttcgc acctttcggc
    12241 gacagccgta tgatgaaaag aattctggtg catctgtttc cgaaacgtga aataaggtgg
    12301 ctgtaccacc gggggagtga aaaaggaaaa cggattatga ggtttccagc gttgtcgaaa
    12361 caataaaaca aggctgcctg aaagtttccc ttcaggcagc cttgtttcag ttctctcgag
    12421 gccattcgta ttttttgttt tcgggataaa tgtcgtcacc tacacgatac caacctcttt
    12481 ttctcatgca ggcatctgct ttacttcctc ctcccccacc tattgggtca tagccgcact
    12541 ctttccagtc ttttttttcc ttagcaaaaa gatcatctat ttgtttgcta tattcttcat
    12601 aagaatatat gcgtaaattg atatttttac tcatcattgc cggatattct tgttctatac
    12661 gggaatactt ccagtatacc gagtcatcgg gcggaggttt gaatccccca aacgaacagg
    12721 cagccaatcc catagccaaa gagattgata cgatatattt cattttgttt ttccttcttc
    12781 tcgaggccat tcgtattttt tgttttcggg ataagggtcg aaacccttac gacaccagcc
    12841 ttttttcctc aggcaggcat ctgcttcaga ctttccgccg tctattgggt catagccgca
    12901 ctcttgcatg tctttcagtt gccttcgtct tgctttcgat ggatattggt caagtgccgc
    12961 cgaggctgat cccggatagg gattggcgta atttttcaat tcccaaaatg acgcggcgtc
    13021 ccacggattt ggtttaaatc ccccaaacga acaggcagcc aatcccatag ccagagagat
    13081 tgatacgata tatttcattt tgtttttcct tcttctcgag gccattcgta ttttttgttt
    13141 tcgggataaa tgtcgttacc tacacgatac caacctcttt ttctcatgca ggcatctgct
    13201 tcactcccac cgcccccacc tattgggtca tagccgcact ctttccagtc ttttttttct
    13261 ctagcaaaag aatcatgcag ttgtcttcgt ctctcttcag gtgggtattg gtcaagtgtc
    13321 gctgaggtta atcccggata aagtttggcg taatttgtca atctccaaaa tgccgagtca
    13381 tcgggcggag gtttgaatcc cccaaacgaa caggcagcca atcccatagc cagagagatt
    13441 gatacgatat atttcatttt gtttttcctt tcggatgttg cagatcataa atgcgtacag
    13501 gacggtacat aatttcgatt tcgttggaca ctcctctttc aaatacacct gtcggacttt
    13561 gggtttgttt attttcaggg tctcgataac catctgttac acattgttta ttagccatac
    13621 cgtagcaatt atgcggcgag gtgtaatccc ctataatatc ccttatagcc tgccattggc
    13681 ttttttgccg ggtattggtt ccgactgttg ccggatttct gccaatcagc atgccgataa
    13741 ggtctaattc gtggttttct atttggatgg actgacgggc gaaatctgcc gttctgggtg
    13801 tcgttacccc ctgctgcagt tgaaacagcc ttttatctgc ccggacaacg ttggcggcag
    13861 ggccgaccat tttcagttcg gtattggata aaattttctt atcccgattg gcttgggtat
    13921 tcaaggtatt gagtacattg tcgaccacca gggttccacg gctgtgcgag ccgacaaaca
    13981 ggccgttttt atccttcccg tagtcttcca tgatattgcc taaagccaag cctgaattgc
    14041 tcaagccgat aacggtctta ttgcctattt ttgccccttc gagcatttta tgaaaggccg
    14101 caacggcgat ttcgggcaat tttgaaaagc cccgcccatt ggtttccgga ttgtgcagga
    14161 aataaacatt ttcataggtg cgttcatatc ggtttttctc gggattgaaa cgccccacat
    14221 attgttgggc ggcaaatttg gcggctgctt gtatattatt gaaaatgccg ttgacgccga
    14281 caacgatttt ttcaacggtt ttgccggtct ccgggtcggt gtaacgggca gttttcagat
    14341 ttttccgttc ctggtcggat acttcgcgca attcgtagat atttccccca atggctttat
    14401 aggcttccca ataatcttta aatgaggcaa attctttttt caactttact gagttgtcaa
    14461 actgtttatt tatatcctct tcaagctgct tatcttcaat cggctcgccg ttttcatcca
    14521 ttttaaaggt catcaggcgg tgttctgcaa taaactggct gcggtaggct tcgtctgcaa
    14581 tgccgccgat cagcgtctca ttgataaact ctttggcaac atccctattg agttcgactt
    14641 ctttcagcaa gccttcgcgg tctgcttttg ccaatgcctt atgtgcggat gaagtgcctt
    14701 tttcaatacc ggcaatattt ttcctgccct gcgcggaagc aatttgccaa tctccctcac
    14761 tgatgacgga gcgggtaatg gattggcggc tttctgattc tttggcattg cccaacagat
    14821 tgcccaaacc catttttgcc aaggcgtatt tgtcgttgct gtgtatatcg ttttgcttca
    14881 aaccgaattt caagccgccg gcgttgtttt gatttaaatt tgcctcttta aaggtttccc
    14941 ctttttcaat acgctcactt cgatattttt cgttcaactc gtcgtctttc gccgaaacct
    15001 ggtcaaaacg catcaggctg accggtttgc cgccgatttt accgatttct gcttcttttt
    15061 tggtggaata ctgtccgctg gtaggcttcg ggctgtatga aaaaccgccg ctcaagccca
    15121 aggcggaagc agccgccgaa gcatggtttt gaatatcttt atgccagatt tcgcttgttt
    15181 tcagcaggtt ttttgatttg tcggcatctg aaacaacagc ggcgccgacc aatccggttt
    15241 tgccgtttac gcgaatccga tagccgtctc ctcctgcaaa gataccgctt tgctcgttga
    15301 cggatgcata atccgagctg cttttcgagc ggttatagct gccaccgaca ctaaagccgt
    15361 agcctaccgt aacttgggcg gaaacatttt cctgtttgcc tttaaacacg gcggtatcct
    15421 gcaaactttc gatatgcaga ctctctgccg ttacgccaac gcctttgcct ttaagctgcc
    15481 cgcctttgat gacggtatcg ccaccgcttt caatagcggt ttggctgtct ttgctgccga
    15541 tatggctgtt gcggtaggcg gtttcgtcgc cgttgccgta acctttgccg tagtttgctc
    15601 cggctgtgaa gccgaaactg atgcctttgt tgatggcgat ggcgactccg gcattaaagc
    15661 ctgcggattt gttttcgctg cgttcctgat gcgtttggcg ggcggcttca atctgaacgg
    15721 cattttctgc tttgaggcgt gttcctttgc cgccgtacac atcggagccg gtaatcgtga
    15781 tgcgggagtc tttgcctgcg cctgaagcag tcagggaaac tttgccgccg ccggtgattt
    15841 tgccctcttg cacctgcgtg cctttgatgc ggctttcgga ggtgttcttc tgttcgccgt
    15901 aggtaacgga gacgctgatg ccctgaccgg ctgctttttt gggatttcgg gcggcattat
    15961 agagtgccac gccggaatct actcctttat tcaaggcgtt ggcagcagcc atggcattga
    16021 cccggctgtt tttgcttttg ccgacggttt ggactgcttt tacggcgtca accgcgccca
    16081 ttacggtatt cacaaccgga acgctaatgg cgacggttac gcctttttgt tcgtaaacct
    16141 gcttactctc ttggctgtaa cggttttgtg cggcatcgat gctgattttt ccggaggaaa
    16201 tgccgacatc gccttggggc gaggatatgg tcgaaccggt ttgggtgtaa tgttttcctg
    16261 ccgaaatcag ggtattgccg ttcaggctgc cgacgacgct ttctgtgtgg ctgacggtct
    16321 cggatcggtt ggtttgcgtg tcttttttgc tgcccgccgt aaagccgatg ccgccgctgc
    16381 ccatcagtcc ggatttttct tttttgttca tttcggcact gcggctgcgt gtttcggctg
    16441 ctttaaggac gatattgttt tttgccgaga gaatggtatg gttgtctgca atgatattgc
    16501 tgccagtaac ggtaatatcg cgtcctgaaa ccagaatgat ttctttgccg tccagcgtgc
    16561 cggatacggc ttgtccgttt tggttcttga gatggcgggt catcttctgt ttgatgccgc
    16621 ccccgcttct accggtgtat ttcagggcat cttcggtttc ggtatgggct ttgccggctt
    16681 cgactttgat atcccgtccg gctgccagtt tcagacggcc ttgttcgctg ccgacctctg
    16741 ctgcacggat acggatgtct ccttttgcat tcagactgag attgccccgg gtgcggatgg
    16801 tgctgccgac ttcgttttgt tctttgcgaa tcacatagtt gtcggaatca aagatagtgt
    16861 tctgattgcg ggaaatgccc gtcgtatccg agcggatgtc gccgccggca ttcagtacgg
    16921 tttgaccgtc ttcagattga ttggtcaatt cggaagccgt caggacgata ttgttgcctg
    16981 catccagcaa gacgcttcca ttctgcctgc cggtcagata aatgcctgcc acccggccga
    17041 tattgcgtac cgagccttgc tcattctgat tgctgcgggt ctcgctgcgg ctttctatat
    17101 tgttactcgc tttgagcagc agggcgtttt ctgcgccgat gctgccggta ttcgtaatgt
    17161 cgctgcctgc tgcggcgaag atgtttttgc cctgcaaatc accttgcaga tttttaatat
    17221 tctgtgcgtt taaaattagt gcttcgcgcc cggcaattaa gccgccccgg ttttcaatgg
    17281 cgccgctgcc gatatcaaca acgctgccgg acagcaacgc cccttgtccg ttcatatctt
    17341 tggggcgtgc gcggacatag actttgggtt tcaatacggt ttgagttgtc ccgtcgggca
    17401 gggtaacggt ctcgttttcc agccaaacaa tgtcggaagt cagacgggca acctgttcgg
    17461 cagacagggc aatacccgga gtaagctgca attctttggc tatggtaatg ccgttatcca
    17521 tcaaagcctt gaattgctct tcgtcattgg tataaccgtc caagcggcgg tagcctgtca
    17581 gcttggcgat ttgttcgttt accagtttct gctcgtaata gccgtcgccc aaacgcttgt
    17641 ggatatggtt cgggtcttgt tgcagtgcgg caagcatata gccgctgccc agccatttgc
    17701 ggtagtcggt aaaggcaggg tcggtttcaa tcaaatagcc tttgttgttt ggcgcaatgg
    17761 caaacaagct gctgttcggc agagtaaatg taggatggat gccgttttca gcaacaacgg
    17821 gtacaacagt gccgggtata tcagatgcct gttggggcgc atagccttta taggctgaaa
    17881 tacccatacg gatggagctg acttcggggg ccggttcgta gggagaccga ctgtatcccg
    17941 tagaatcccg ccctttcttg tgatgacggt ggtaacggtg caagtcccct ttatcggtta
    18001 tggtttttgt tcccaaagtt tcatcattgt ccaatgcgga ttcgggcgta ccgacgatca
    18061 atctgccgcc ggcaataatc cggctgttat gatttttcag cttggcggca tctaaaacca
    18121 aattgccgcc actgatgatt tgaccgggct tggattcggt aattttgctc tcggcggtga
    18181 ttcgttcgta atcgtatttg taccagacag agtgggggct gccgtccgga gtccgcatat
    18241 gtaaagactc atcttcaaat atttcccagc ccaactcttt ttgagaacct tccggatagc
    18301 gttctgttct gccttccgcc tgatattcga tacggtgttc gcgatgggtt tcttccgtat
    18361 ggaaacgcag atgctcattg gtattctgca aatcttttgt agcgatacgg atattgcctg
    18421 atgattcgat tgccgcactg cggttgtgca gtgatgtatt tgctccttgc acctgtcggc
    18481 ttccattcaa tgcagaaccg atatgaagat cgccggagct ggacaataat gccgcctctc
    18541 ggttctcaat ttcccgcgct ccaatatcca accgctcccg cgctgcaatt accgccgctt
    18601 tggtttcgcc gttgaccgtt tcttcccggt tcaacaaggt atctgcctca actgccacac
    18661 ggctgccgta aattctgccc gtgccggcat tgtccgactt ggcttcggtt tgcagcagcg
    18721 ttataccgtt gctgttgatt aaacccctgt tggtgatgcc gtttttgccg tttaacccgg
    18781 tgcggtttcc ggattggatt ttaccggaga cagtgttgtc gatttgggcg gcggtcaggg
    18841 atacggtatc gcctgcctgt atgatgcggg tgtttttcag acggccttgg gtggagaacg
    18901 tgagtgtgcg tccggcttca atatcgcgtt tgccggcaaa atcatcatga agagaaacgg
    18961 aaacatcgcg tgcggcggtt aatatgccgt cgttgtccag tgattttgcc tgtaaggaaa
    19021 catctttgcc cgcaataatc gtgccgtctg tgttattgat atgcaggctg cttttgcctg
    19081 tatcgcgaat atccagcaaa ccggccgagc cgattaagcc tgcttgatta tttatgccgt
    19141 ctgaaacctt caatacactg cctttgccgc tgcggataaa gccttggcgg ttatcaacgg
    19201 tttgagcgga aatggtgatt tcggcagcat caatcgaacc gctgttgctc aatttgccgt
    19261 ctgcgcttaa cgtaacgccg cctgttgcgg caaaaatccg acccttgttg cggattacgg
    19321 cgccgttgtc ggtgctgatt aaagtgattt tgtctgcgta catcccaccc agtgtggcgg
    19381 tgtcgatggc aacggtagga gtaacagaat ccgaagaaga tggcgcagaa gctgttttgg
    19441 caagagagcc gtcaaaatcc aatttgttct tacccgaaac caccttgaca tctttacccc
    19501 aaacgcccgc attgatttca gcagcacgac taaggatacg ggtgtaatcg gcatcagagg
    19561 tatccaaacc tttgccccca atcacgactt tacccgaaga aacatcaaag cccgtcagat
    19621 tgccgttatt caaaacagga acgcccgaag tcagcgtaac cgaagcggca ttaatcaatc
    19681 cgccgccatt cacacggatg cccgacggat tggcaacgac tacttcggcg cgtttgccgc
    19741 cgacttcgat ataaccgttc aacaacgaag gattactgct gtcaatctgg ttcacaatta
    19801 cccgcgcttc gccgcgtgcc agatggggat tgccttgaat ccatccaccg agttgcgttt
    19861 gcgtattgct gcggctgttg tttagtatta cgcctttttc atcaacatcg aactgcttga
    19921 atcggttaac agaaacgcct tgggatgacg gagtttgaat attgacttgc ggcaaaccgt
    19981 ttgctgtctg aagaataacg gcttgttggt ttttaggggc ggatttgtcg gcaatgatgc
    20041 cggaagcagg ggcaggggaa aacgcagcaa cacccaaagc caacatgaca gaaaaggcag
    20101 ccatacggaa accgaaggct gcccgggcag aagaaacaga agcggcaccg gtcactcgaa
    20161 ccgaagccgc ctcactatcc tgcatactct tgccgtcacg atgaacattc tctgctacag
    20221 ccatcataca actgcgtttc ttgttgaaga taaccttgta gcatcgcttg ttcatgatgg
    20281 gttttcttaa atgaaatgta aggaatagtt aaggacaaaa tataggaaat ttgaattaaa
    20341 ttgtcaataa aaaactgaca gcgtaccctg ataatcagat gttgtacgct aattgtgaga
    20401 agtatgtcgg gatgattatt ttgaagtttt ctttttattc aaaagagtta ctatgaaagt
    20461 taaccgggcg taaataggct tcaataaaca ggttatcgtt atttttcaat tgggaaaaaa
    20521 tcttccctaa cccatccatt cgccaaatta ttatgtggga attcggcaat ttcaataacg
    20581 gctgttgatc catatctgct cggtcattta gcgttataaa gtcgcaaata gcgtcagaat
    20641 ttacaagatt atcggatttt gggaataaat tatgccgtct gaagggcttt cagacggcat
    20701 aggcggctaa cacgggtgcg gcttgcgcca aacggcgcgg caggcgcagg gaatcgcttt
    20761 ttactttggc gcggtgttcc gggtttaatg atgcccgcaa ccgccgtggc tttccctcaa
    20821 ggcttccgcc gcctgttcgt cggcgtggta gctggaacgt accatcgcgc cgatggcggc
    20881 attgctgaag cccagttcgt atgcttcttt ttcaaagatt ttgaactgct cgggcgtaac
    20941 gtagcgcagg acgggcaggt gtccgtctga aggctggagg tactgtccga tggtaatcat
    21001 ttcgatattg tgcgcccgca tatcgcgcat aatttcacgc acgtcttcgt ctgtttcgcc
    21061 caagccgacc atgatgccgg atttggtcgg gatgtgcggc atcatttctt tataacgttt
    21121 taataagtct aaagaatgtt gataattggc accgggacgg gcttttctgt acaggctcgg
    21181 atgggtttct aggttgtggt tcatcacgtc gggcggggtt tcggcaagga ttttgagtgc
    21241 gatgtccaag cgtcctcgga agtcggggac gaggatttcg attttggtgt tcgggctggt
    21301 ttcgcggatg gctttgatgc agtcggcgaa atgctgtgcg ccgccgtcgc gcaggtcgtc
    21361 gcggtcgacg gaggtgatga cgacgtaacg caggttcatg gctttgacgg attcggcgag
    21421 gtttctcggt tcgtcggggt cgagcatatt gggccgaccg tgtcccacgt cgcagaacgg
    21481 gcagcggcgg gtgcagatgt cacccataat catgaaggtc gccgtgcctt tgctgaagca
    21541 ttcgccgatg ttggggcagg aggcttcctc gcaaacggtg tgcatctttt gttcgcgcaa
    21601 aatgtctttg atttcaaaga atttgcgcga tgggagtttg gcgcgtatcc attcgggctt
    21661 tttcagtttt tcctgaaggg ggacgacttt gatggggatg cgcgcggttt tgtccgcgcc
    21721 tctgagtttg atgccgcgtt tggggtcgtc ggttttgatt tcactcattg ttgtctgctt
    21781 tcggtgtgaa ttgtgtttca aggtgtgcgg tgagtttggc ggcgacttcg tccggcgtgg
    21841 ggcagggttg gacaaaatcc gcgatttgcg tcatttccat accggcgtag ccgcaggggt
    21901 tgatgtgggt aaacgggctt aaatccatat tgacgttgag cgcaagcccg tgatagacgg
    21961 agccgttttt gatacgcagc cccagtgagg cgattttgcg ttctccgaca taaacgccgg
    22021 ggcgtttggg gtctgccgcc gcttcgatgc cgtattctgc caatgtggcg atgatgctgt
    22081 tttcaagcgc ggaaacgatg tttctaacac tggttttgcg ccgtttgaaa tcaatcatcg
    22141 tataaacgac caattgcccg ggcccgtgat aggtaatctg cccgccccgg tcgatttgga
    22201 cgacgggaat gtcgtcgcga atcagcaggt gttcgggttt tcccgccagt ccttgtgtga
    22261 acacgggcgg gtgttcgacg acccacagtt cgtcttcggt gtcggcattc cgtccggcat
    22321 taaaggtttt catcgcttca aaagtcggca gatattcgac caaacctttg tgtatgattt
    22381 tcatctcaaa gtaccacttt gaccagttcg tgcgaagtca gcgcacggta gatgttgtcc
    22441 aattgttctt ggttttcaac ctttacctgt acggtggcgc cagtatagtt gcctttgctg
    22501 ctcggacgcg tggtgatgtg gtgcgcctgc gtgtcggggg cgtggaggcg gacggtgtct
    22561 aaaaccgcct gctcgaactc gggatgcacc gcgcccatta ctttcaatgg gaaggtgcag
    22621 ggaaattcga tgagggatgt tttgtttttt tgttcggtca tgatgtgctg ccttgtcgtg
    22681 tacggtatgc cgtctgaagg cgggtttgcc tttcagacgg catcggatgt gcgttatttt
    22741 agcctaaacc gcgataacag gctatcggga aggcggaggc ttttttgacg gcgcggcggt
    22801 tctgctatac tggcgcacaa tattattttt agaagggtgg tttttatgta tcggaggaaa
    22861 gggcggggca tcaagccgtg gatgggtgcc ggtgcggcgt ttgccgcctt ggtctggctg
    22921 gttttcgcgc tcggcgatac tttgactccg tttgcggttg cggcggtgct ggcgtatgta
    22981 ttggaccctt tggtcgaatg gttgcagaaa aagggtttga accgtgcatc cgcttcgatg
    23041 tctgtgatgg tgttttcctt gattttgttg ttggcattat tgttgattat cgtccctatg
    23101 ctggtcgggc agttcaacaa tttggcatcg cgcctgcccc aattaatcgg ttttatgcag
    23161 aacacgctgc tgccgtggtt gaaaaataca atcggcggat atgtggaaat cgatcaggca
    23221 tctattattg cgtggcttca ggcgcatacg ggagagttga gcaacgcgct taaggcgtgg
    23281 tttcccgttt tgatgaggca gggcggcaat attgtcagca gtatcggcaa cctgctgctg
    23341 cttcccttgc tgctttacta tttcctgctg gattggcagc ggtggtcgtg cggcattgcc
    23401 aaactggttc cgaggcgttt tgccggtgct tatacgcgca ttacaggcaa tttgaacgag
    23461 gtattgggcg aatttttgcg cgggcagctt ctggtaatgc tgattatggg cttggtttac
    23521 ggtttgggat tggtgctggt cgggctggat tcggggtttg ccatcggtat gcttgccggt
    23581 attttggtgt ttgtccctta tctcggggcg tttacgggat tgctgcttgc caccgtcgcc
    23641 gccttgctcc agttcggttc gtggaacggc atcctatcgg tttgggcggt ttttgccgta
    23701 ggacagtttc tcgaaagttt tttcattacg ccgaaaatcg tgggagaccg tatcgggctg
    23761 tcgccgtttt gggttatctt ttcgctgatg gcgttcgggc agctgatggg ctttgtcgga
    23821 atgttggcgg gattgccttt ggccgccgta accttggtct tgcttcgcga gggcgtgcag
    23881 aaatattttg ccggcagttt ttaccggggc aggtaggcgg ttccgaaaca tatagtggat
    23941 taacaaaaat caggacaagg cgacgaagcc gcagacagta caaatagggc aacgccgtac
    24001 tggtttttgt taatccacta tatttgaagc ggaatacaac cttgcccggg tttaaataaa
    24061 aatgccgtct gaaccccgaa aactggactt cagacggcat tttcatcacg gcttatttgg
    24121 cggttttgct gctgtcgata attttcatac cggcagaaat caggctgccg atgtcggcaa
    24181 cattggcggg cataatcagc gtattgcttt ctttggcaag attgttgaac gcagcgacgt
    24241 attgttccgc aatcttcaga ttgaccgcat ccgcaccgcc ttgggtttga agggcggcgg
    24301 caatttgacg gatggcttcg gcattggctt cggcaacaag gcgcaaggat tccgcttcac
    24361 ctttggcgcg gttgatgcgg gcgattttct cggcatttga cgcattgacc gcagcctgag
    24421 cctcgccttc ggattgttgg atttcggctt cgcgctgacc actggcaagg ttgatttgtt
    24481 cgattttacg accttcggat tcggcgatac gggcgcgttt ttcgcgttcg gcagtaattt
    24541 gcgcctgcat tgagcgaagg atttcttgcg gcggaaccaa gtctttaatc tcataacgca
    24601 aaaccttcac accccaagcc ccggccgcct cgtccaaagc cgcaacaaca gtactgttga
    24661 tttcgtcgcg ttcttcaaac gttttgtcca actccatacg cccgataacg gaacgcagcg
    24721 tcgtttgggc aagctgggta atcgccataa tgtagttgct cgaaccgtat gaggcgagtt
    24781 tggggtcggt tacttggaaa tagatgatgc cgtcaacagt cagctgcgta ttgtcgcgcg
    24841 tgatgcagac ctggctgggt acgtctaaag ggatttcttt cagcgaatgg cggtaggcga
    24901 cgcggtcgat aaagggaatc aaaatattca aaccggccgt cagggcgcga tggaaacgcc
    24961 ccagcctttc gacaacgtgg acttcctgtt gtgggatgac aacaaaggat ttgaaaccga
    25021 aaacggcgac ggctaccaac aagataatga aaaattccat aattcctccg agtgttaagg
    25081 gtgtgtgata ataagaaggt tgccttcctt gcggacaatg agggcgcgag ttcctggttc
    25141 aagctcttct tgccccgtat tttgagcctg ccagtgcgta ccgcgataaa aaacttcgta
    25201 acggttgccg cctgtgtgtc ggaggatttc gacatattgt ccggcatcca aatcctgata
    25261 tgaatccgtt tcaacttttc taacggcggt tttggcgtgt acgaaccaaa tacccagcgc
    25321 ggaaagcaga gcggcggtca agacggcggc aggcgtactg ccggtcagcc cgtaagcaat
    25381 gcccgaaccc gccaaagccg cgctgacaac caaaagataa accgttcccg tcaataattc
    25441 gatgattaag acggcaacag cggcaacaaa ccatacagtc atacatttcc ccacaaagtg
    25501 cgtcgtttga caaaatcacg cgatatcagc agtatagccg aatttgaaag gatagggcag
    25561 atatggatac ttggcacgat gcactgggca acgaaaaaca tcaaccgtat tttcagaaaa
    25621 ttttaaatgc ggtcaggcag gaacgtttgt cgggacaaat catctatccg ccggagactg
    25681 atgtgttcaa cgcattccgc ctgacagcgt tcgaccgggt caaagtcgtt attctcggac
    25741 aagatccgta tcacggggca gggcaggcgc acggtttggc attttccgtc cggcagggtg
    25801 tccgcatacc gccgtcttta ctcaatatct acaaagaatt ggaaaccgac atcaacggtt
    25861 tttccattcc cgatcacggc tgcctgacgg cttgggcgga gcagggcgta ttgcttctga
    25921 acacggtttt gacggtgcgt gcaggacagg cgcattcgca cgccctttta ggctgggaac
    25981 gctttaccga taccgtcatc aggcagcttg cggcgcaccg caagcacctt gtcttcatgt
    26041 tgtggggcgg gtatgcgcaa caaaaaagga aactgataga cagtcaaaat catttgatac
    26101 tgaccgcacc gcatccgtct cctctgtcgg catatcgcgg ttttttcggc tgccgccatt
    26161 tttcacaggc aaacagctat ttgagccggc acggtatcga tccgataaac tggaagctgt
    26221 gaatgccgat atagccgttg ccgccggcgt gttaaaatcg cgtttgattt gtaatttcca
    26281 tttattaggc aaaaccttat ggcaatcaaa aaaaaatcag ctttatgcat cactttgggc
    26341 gagctgcgac gagttgcgcg gcggtatgga tgcgagcctt tataaagaac tatgtgctta
    26401 cgctattgtt tttaaaatat gtttctgata agcataagta cggcggcggc atgattgagc
    26461 tgcacgccgg tacgactttt gacgacatcg tcaaactcaa aaacaccgcc gacatcggcg
    26521 accgcctgaa taagattatc gcccaaattg ccgaagccaa cgacttaaaa ggcgtgatcg
    26581 acgttaccga cttcaacgac gaagacaaac tgggtaaagg taaggagatg atcgaccgtt
    26641 tgagcaggct tgtcggcatt tttaaaaagc tcaacctttc ttccaaccaa gccgaagacg
    26701 acgatttgtt aggtgatgcc tacgaatacc tgatgcgcca ttttgcgacc gagtcaggca
    26761 aatccaaagg gcagttttac acgcctgccg aagtctcccg cattatggcg aagattatcg
    26821 gaatcagcgc agattgccgt ccagcaccag cgtttatgac ccgacctgcg gctcgggttc
    26881 gctgttgctc aaagccgccg cccaagccgg cagccaaatc agcctttacg gtcaggaaaa
    26941 agatgtggca accgcgtccc ttgcccgtat gaatatgatt ttgcacaaca acgaaaccgc
    27001 cgaaatcaac accgggaaca ccttgtccga ttcgtctttc cgtgatgaaa acgacgggct
    27061 taagaccttc gattttgccg ttgccaatcc gccttatccc gcccgaaaaa aacggcgatt
    27121 acgccttttt gctgcatctg ctcaaaagcc tgaaaccaag cggcaaaggt gcgattattc
    27181 ttccgcacgg tgtgctgttt cgcggcaatg ccgaagcgcg tattcgcacg gaattgctta
    27241 accttgacct tattaaaggc attatcgggc tgcctgccaa cctgttttac ggcacgggca
    27301 ttcctgcctg catcatcgtc atcgacaaag aacacgccca aaccgcccaa tttgccgaag
    27361 agggaacaaa ccaagttatc agtggcggca gcgtgtttat gattgacgca tcgcgcggct
    27421 tcattaaaga cggcaacaaa aaccgtctgc gtgagcaaga cattcacaaa atcatcgaca
    27481 ctttcacaaa cctcgttaca gccgtatggt gcatttaagc gaaatcgcag cacaagatta
    27541 caaccttaat ctgcctcgct atatcgacag cggagaagtt gaagacctgc aaaatctcgc
    27601 cgcctatctt tatggcggca tacctgcgca cgatatggac gcattggaag cctattggca
    27661 agttttaggc cgtatgaaaa acgagttgtt tgccgaacac gatggccgct ttaccactat
    27721 acaacggaat cgattgcaaa tctttcccac tctcaacagc ttaaaatcct gcgggattgg
    27781 tgtggaattt agggctaatc tagtacagcc ccaaatttaa tccactataa aatcgaaagc
    27841 agccaaatca aagcccatat attggcgcac cccgattacg ccgccttcaa agccggacac
    27901 ctggcaaagt ttgccgcgtg gcacactcaa aacgaccttg ccgccatcca accgggcagg
    27961 cttatccgga aatggagcga aagcctgctg gacgcgttca aacccggcag cctgattgaa
    28021 gaatacgatt tctaccaaat cctgacggac tactgggcgg aaaccctgca agacgatgtt
    28081 tatctcatcg cccaagatgg ctggaaggcg gttaaaaacc tggccgaaat caccaaagaa
    28141 agcgatgaag acgcgaacct gaccgtcgtc tttgaggaaa ccgaaaccgg caaaaaaggc
    28201 aaagccaaaa ccaagcgcat cagcaaaaaa taccgcagcg aagtcatcgc ccccgagctg
    28261 gttgcccgcc gctacttttc agacggcatc gccaagctga aagaaaaaca aagcgagctg
    28321 gaacgcctaa gccaagaatt ggaaaaccac atagaagaac acggcggcga agagggtgcg
    28381 ctgaacgacg tattggatgc aaaaggcaaa ctttccgcca aacttctgaa aaccgcattg
    28441 gaagaaagcg gcatagaaga aggcgaacgg gctgttttac aaaccaccca aacactgatg
    28501 acgcaggaaa aagccgcgaa agacgcagtc aaaacccaaa tcgaagccct gaaccttgcc
    28561 gtattcaaac aattcggccg actttccgaa gccgaaatca agcagcttgc cgttcaagac
    28621 aaatggcttg ccgatttaca aagccgaatc gaaaatcgct tggaaaacag tattcagcag
    28681 cttatcagcc gcttgaacac gctggaagac cgctaccgca gcccgatggc cgagcttgcc
    28741 cgagaagtgg aaaagtggca aagcaaagtc aatgcccacc ttgaaaatat gggttttgga
    28801 ggctgaaatg gcggcacaga caggctataa ggcgagcggg ttttgagacc tttgcaaaat
    28861 tccccaaaat cccctaaatt cccaccaaga catttagggg atcgcggttc gggtgtccgc
    28921 accgcttaat acgtcgtgtc cacgaactaa cccatttgct cgaacgccat cgcaacgccc
    28981 gttttatggc gcatatggac aactttctcc caaactggca aagcatcaaa caacagctta
    29041 atgccttgga gttatttgca caaatatata atttaacata atatacatta tgcgaactat
    29101 cggaaacagt tgtacgtgtc cctgtggtct ttccaagtag gaaaattaaa gtatggccaa
    29161 tgcggctgaa tgtatagccc ggagcatccg cgtatccgaa ggtactgaac gacatgccct
    29221 gcatcccttt cgacgcttcc atccagccgg tattgggaaa tacgttgacc ttcaatataa
    29281 tatacaggca gccgacacac aggatatgcc gctgcttttt catcatttct tctgtcaaat
    29341 ccttggaacg gtcgatttga aaacacggct ttacatacgc cccagttccc tttgcagggc
    29401 ttgaatattt tgctgcctgt ccaatacatt gctttgtaat gcatttattt cttgatggtt
    29461 gatgttgccg ccctttgcca gacgtgcttg tgataacatt ttttgggctt caaccaatgc
    29521 tttgcgttcg ttgctcaatt ctgtttcgag aatggagcgt ctgctgttgt ttgagggtgc
    29581 ttgttgcggc ggcggcgtat tggattttgc cggcttggat actgttttga ccggggcttt
    29641 atatttgaca acctgtccgc cgtttgacgg tgatgatacc ggttcgggcg tttggggcgg
    29701 gatatagcgt tcgctgctgt agttgccgat tgggggcaaa tcggttgagt ggcagctttt
    29761 ggacggcttg gtggtgtaaa cggtttctcc gttgattgtg caggtgtaga ttttggccgc
    29821 attcgcaccc aatgggcttg aaatcaggga aaagttgatt aggattaagg ggagttttga
    29881 tttcataatg tgcttgatgc tcggataatt atttgatttt ttggtatttt ttataatgct
    29941 ttcttggcgt tttacgggct tttcttttcg gttgtttgct tttgattttc tttcggggct
    30001 ttggcagcct gcttgttctt tttcccggca ggttttttgt ttttcttttt actttccatg
    30061 tgtattttgg cttcttaact gagtttttta attttcaggc ggtatccgcc tccctgatgg
    30121 ctgctgattt taggtaaatc cgcatcggcg cacgatcctg ctggggctga tgtatatacg
    30181 cttttgccat ttgagttgca atggtatacg gaggcttgcg ccgccgcgcc ggaaagggac
    30241 aggagaccca aggcggcaaa aagtctgatg tttataccgg taaaaggcgg tgataacgcc
    30301 cgaattatac cgttattggc aggcaaagat aagcaccctg tccgcgcttc tttatcgctc
    30361 ggcaaactgt ttctcgattt cagtttcaat ccgttccaat tcttcttgac aagccagcca
    30421 aacctcttcg atttgggcaa gttgtgtttt gacttttgcc agcagggata aggtattttg
    30481 caatttttct ttgtttgctt caaggtaagc gtcttcttgt gctaaaaatg cttcacatgc
    30541 cgtctgaatt tcggaaagct gcgccatttc tttttcggca cggtctattt tctgctgtat
    30601 cggcttgccg cgtcgggctt tttcctgacg gatttgcgct tcgatgcgct tggtgtcttt
    30661 gcggctttgg ctttgtgcgg atgctgctgg tgcggcggcg ttttcctgtg ccaaacgcca
    30721 ttggcggtaa tcgttcaaat cgccgtcgaa gttcttcaga cggcctttgt cgatcaggag
    30781 gaagctgtcg gtagtcgctt caagcaggct gcggtcgtgc gatacgatga ttaaggcgcc
    30841 ttggaaactt tgcaacgcga gcgttaaggc gtggcgcata tccaagtcca aatggttggt
    30901 cggttcgtca agcagcaaca ggttcggctt ttgccagata atcatggcaa gggcaaggcg
    30961 ggctttttct ccgccggaaa aaggttcggt tttctgtaac gccatatcgc caacaaaatt
    31021 gaagccgccg aggaaatttc ggatttcttg ttcgcgtact tcgggagaaa gctgctgaat
    31081 atgccaaaca gggttttggt cggcgcggat ggtgtcgagt tggtgttggg caaaatagcc
    31141 gatattgagt ttttcggaac ggatgatgct gccggaaagc aaatcgattt tgcctgccaa
    31201 ggctttgata aaggtggatt tgccgctgcc gttgacaccc aatagcccgt agcgtgcgcc
    31261 gctttccagc gacagggtaa tgtcgtgcag gacagttttg ccttcgtaac ccaaatcagc
    31321 gtgttctagc tttaacaaag gattgggcag atggtcggga tggtaaaact caaaggaaaa
    31381 ctcgctgtcc agatgcgcgg gagcgatgcg ttcgagcttc gccaaagcct tcatgcggct
    31441 ttgcgcttga acggctttgg tggctttggc tttgaagcgg tcgataaagg attgcaaatg
    31501 tttgatttgc gcctgctgtt tgacataggc agcttgttgt tgcgcgagac gctgcgcacg
    31561 ttcgttttgg taaaaatcgt aattgccgcc gtattgcgtg agtttttgct gcgataattc
    31621 aatggtttgg gtagttgccg cgttgagaaa atcgcggtca tgggaaatga tgatttgcgt
    31681 gcagggtaaa gaagcaaggt ggttttccag ccacaagacg gtttccaaat ccaagtggtt
    31741 ggtcggttcg tcaagcaaga gcaaatcggc gcggcaaatc agggcttgcg caagattcag
    31801 gcgcatacgc cagccgccgg aaaaggattt gacggggcgg ctgtgttctt cttgcgaaaa
    31861 acccagcccg ttcaacaatt ttgccgcacg cgccggcgcg gtataagcgt cgatttcttc
    31921 caatttagca tgatattccg cctgcttcat gccgtcattt tgcgcttctg cctgcctcaa
    31981 tgccgtctga aaagcctgca actcggcatc gccctgcaaa acgtaatcca aagcggaaat
    32041 atccaaatcg ggcgtttctt gggaaacgga agcgagccgc cagtttttcg gaatcgagac
    32101 atcgccgccg tcctgagtga tttcaccctt gattaaggca aacaggctcg atttgcccgt
    32161 tccgtttttg ccgatcaaac cgacgcgctg accgggattg acggtagcgt tggctttgtc
    32221 gagcaggact ttcaaaccgc gttgcagggt gaggtttttg atttcaatca taacggaaac
    32281 atcgtcgggc gggaaaagcc cgtattttac ctgaaagtca gtgccgatgc cgtctgaaac
    32341 gggaaattta cggctgaagc caagcccaag ccctgcgccc ttccgagtgc aggaaaacca
    32401 atgtcctgaa tgccgaatcg gtattcatgc attccacgct gattccgatt cgggaaaaat
    32461 ccgctatgat tttgggatgg ataaactcct gagccgcgcc cgtcccgata atcaatattt
    32521 ccggatagtc aacaggtttg acgtcggaca acaggttttc cggagtcaga tcggacaagg
    32581 ttcggcattg cgacaggcag accgaatcct tatgtacaag cacgggttta tggaaacttt
    32641 gccccgccag ccggattccg cccgcaccgc attcatattc cgcaaactgt ccgtctatcg
    32701 gattttcttc aaacaacatt tttttacccc gttgccgcat catctacacc gaaagggatg
    32761 caaaatcaga caaattcatg taggattggc agatttcatc tgacccgcct gccgatttca
    32821 gacggcattt gattcaaagt gcggcacaat tatatcggca gcggatattt tcgtctttca
    32881 atatttacat ttcagtcggc ttacaaggag acacaatgaa gccagtaaac atcggtcttt
    32941 taggtttggg tacggtcggc ggcggtacgg ctgccgtgtt gcgggacaac gcggaggaaa
    33001 tttcccgtcg cttggggcgc gaaatccgta tttctgccgt gtgcgatttg agtgaagaaa
    33061 aagcccgaca aacctgcccg tccgcagcct ttgtcaaaga tccgttcgaa ctggtcgcac
    33121 gtgaagacgt cgatgtcgtc gtcgaattgt tcggcggtac cggcattgcc aaagatgcgg
    33181 tgttgaaagc cattgaaaac ggcaaacaca tcgttaccgc caacaaaaaa ctgctcgccg
    33241 aatacggcaa cgaaatcttc ccgctggcgg aaaaacaaaa cgtcatcgtc caatttgaag
    33301 cggcagtagc gggcggtatc ccaatcatca aagccctgcg cgaaggtttg gcggcaaaca
    33361 ggattaaatc catcgccggc attattaacg gcaccagcaa cttcatcctc tccgaaatgc
    33421 gcgaaaaagg cagcgcgttt gccgatgtac tgaaagaagc gcaggcattg ggttatgccg
    33481 aagccgatcc gaccttcgac atcgaaggca acgatgcggg ccataaaatc accatcatga
    33541 gcgcactggc attcggcacg ccgatgaact tttccgcctg ctacctcgaa ggcatcagca
    33601 aactcgacag ccgcgacatc aaatacgccg aagaacttgg ctatcgcatc aaactgttgg
    33661 gcattacccg caaaaccggc aaaggcatcg agctgcgcgt ccaccctacc ctgattcccg
    33721 aaagccgcct cttggcaaac gtcaacggcg tgatgaacgc cgtgcgcgtc aacgccgata
    33781 tggttggcga aaccttatat tacggcgcgg gcgcgggcgc attgccgacc gcttccgccg
    33841 tggttgccga tatcatcgac atcgcccgcc tggttgaagc cgataccgcc caccgcgtac
    33901 cgcatctggc gttccaaccc gcgcaagtcc aagcgcaaac catcctgcct atggacgaaa
    33961 ttaccagcag ctactacctg cgcgtccaag ccaaagacga accgggcacg ctggggcaaa
    34021 tcgccgcgct gttggcacaa gaaaacgtgt ccatcgaagc actgattcaa aaaggcgtga
    34081 ttgatcagac cactgccgaa atcgtgattc tgacccacag cacggtcgaa aaacacatca
    34141 agtcggcaat cgcagccatc gaagcactgg attgcgtgga aaaaccgatt accatgatcc
    34201 gcatggaaag cctgcatgac tgagccgaaa cacgaaatgc cgacggaaga gcaggttgcc
    34261 gcgcgcaaaa aagcaaaagc caaaatccgc accatccgca tttgggcgtg ggtcattttg
    34321 gcgttgctcg ctttaaccgc cctgctctcc caatgcgcga tgtccaaacc gcaggcaaaa
    34381 cagaaaattg tcgagtcttg cgtgaagaat attccgtttg ccgaaaaatg gcaaaacgat
    34441 ttgcgggccc gcggtttaga ttcaaacaat acccgcctcg ccgtcgacta ctgcaaatgt
    34501 atgtgggagc agcctttgga cagattgagc gagaaacaga ttagatcctt cggcaaactc
    34561 ggcgcacaag aacagcttga cctgctcggc ggcgcaaatg cctttgaagc acgtgacaag
    34621 cagtgtgttg ccgatttgaa atcagaataa tgtggaccga taaaaaagcc gattctttaa
    34681 agaatcggct tttttcataa aaaacggctt acagtgcatc tttcaatgct ttacctgcac
    34741 ggaatttagg cgttttggcg gcggcaatgg tcagaggctc gccggttttg gggttgcggc
    34801 cttggcgttc cgcacgttcg cccacgtaga aagtaccgaa accgaccaaa gtaacggtgt
    34861 cgccttgttt cagggcggtg gttactgcat tggtagtggc atccaaagct ttttgtgcgg
    34921 cggctttgga aatgtcggct tcttgagcaa tcgcttcgat caattcagac ttgttcacaa
    34981 tcagtccctt cctgtcttaa aaaatgatga aatgcccgaa tactcggggt ttgtactgct
    35041 tgagcaactt tcgctttata gcaattctga aattgccgtg tcaagcaaaa aatacggaat
    35101 caccctattt gacaggcttt caggacgaaa ccgcattttt acaacacatt tcctgcgttt
    35161 caatgtttgg ttgccctgct gcggggtttt ggttttgaag cggattccgc cgccgcttcc
    35221 gcaccagaag gttctgccca aggctcaggc tggctttcca aacccagagc caatacctcg
    35281 tctatccatt tgaccggatg gatggtcagg ccggttttca cgttttcagg gatttcttcc
    35341 aagtctttga ggttgtcttt tggaattaaa acgtgtttga tgccgccgcg aagcgcagcc
    35401 aacagttttt ccttcagacc gccaatcggc aggacttcgc cgcgcagggt aatttcgccc
    35461 gtcatggcaa catcggcgcg caccgggatt ttggtgaatg cagacaccat cgccaaagtc
    35521 atggcaatgc ccgcactcgg accgtctttc ggcgtcgcac cttcgggaac atggacatgg
    35581 atgtcttttt tctcgtaaaa atcaggagcc aagcccactg attccgcgcg ggagcggaca
    35641 accgaccacg ctgcggacac ggattccttc atcacatcgc ccaactggcc ggtgcactga
    35701 atcacgccct tacccggcaa tgctgcggct tcgacggtca gcaattcgcc gccgacttcc
    35761 gtccacgcca aaccggttac ttgtccgata cggttttcgc tttcggcaac gccgtaatcg
    35821 aagcggcgca cacccaaata gtcgtgcaga tttttctcat ttactttaac cgccctgggc
    35881 ttggcttttc cggttttttt ggtttttgag tcttcgtcca aggtaatctg catcaccacc
    35941 ttgcggcaga ttttggcaat ctcgcggtcg agcgaacgca cgcccgcctc gcgggtgtag
    36001 taacggataa tatcgcgcac cgcgctttct tcgattgcca attccccttc ttttacgccg
    36061 ttgcgcttca tttgcttcgg cacgaggtac tgcatcgcga tattgatttt ttcgtcttcg
    36121 gtatagccgg acagacggat gatttccata cggtcgagca gcggtgtcgg gatattcagg
    36181 ctgtttgaag tcgcaataaa catcacatcg ctcaaatcgt aatccacttc ggcataatga
    36241 tcggcgaact tgttgttttg ctcgggatca agcacttcga gcaacgcgct ggcgggatcg
    36301 ccccggaagt cgctacccaa tttgtcgatt tcgtcgagca ggaacaatgg gttttttacg
    36361 ccggcttttg ccatattctg caaaatctta ccgggcatgg agccgatata ggtgcggcgg
    36421 tgtccccgga tttcgctttc gtcgcgcacg ccgcccaaag ccatgcggac atatttccgc
    36481 cccgttgctt tggcgatgga ttcgcccaaa gaggttttgc ccacgcctgg agggccgacc
    36541 aggcacagaa tcgggccttt gagtttgtcc atacgttttt ggacggcgag gtattccaaa
    36601 atccgttctt tgactttttc caggccgtag tggtcggtat ccagcaccaa tccggctttg
    36661 gcgatgtctt tgctgacgcg ggattttttc ttccacggca gttcgagcag ggtgtcgatg
    36721 tagttgcgta cgacggtgga ttcggcagac atcggcggca tcattttgag ttttttcagt
    36781 tcggacaggc atttttcttc cgcttctttg ctcatccccg cctttttgat gcctgcttcc
    36841 aaggcatcca gttcgccgtt ttcgtcttct tcgcccaatt ctttgtgtat cgctttaatc
    36901 tgttcgttca gataatattc gcgctgggat ttttccattt ggcgtttgac gcgtccgcgt
    36961 atgcgttttt cggcctgcat aatgtcgagt tcggattcca gctgtgccag caggaattcc
    37021 atccgtttgc cgatttcggg aatttccaaa atctgttggc gttgcgccag tttcaactgc
    37081 aaatgcgctg cgaccgtatc ggttagccgg ctgttttcgg caatgccgtt aatgctgccg
    37141 ataatttcgg cggggatttt tttattgagt ttggcgtatt gttcaaactg cgccaacagg
    37201 gtgcggcgca cggcttcgag gtcggtattg ccgcccgtgt cttcttccac gaccgtctct
    37261 atatgggaaa cgaacagacc gcccgtgtct tcaatggtca gaacacgtcc gcgatacagc
    37321 ccttcgacca atacttttac cgtgccgtcg ggtagtttca acacttgcag gacttgtgcg
    37381 accgtaccgg tctgatacag gtcggcggca accggttctt ctagcgccgc atcggtttgc
    37441 gccaacagga aaaccggctc ctcgcgggta atggcgtttt ccagtgcggc gatggatttc
    37501 ggcctgccga caaacagcgg cagaaccata tgcgggtaaa cgacgacatc ccgcaaagga
    37561 agggttgcca aggcggcata ttcctcaaaa tgcttttctt tttgtgtcat aggtactctc
    37621 ttgtgtctga cagattgccg attttcgcgt acattggggt tgaaggtatt atttcaagca
    37681 tatgtggttt atttatggag tttgatgcga tgccgtctga aacattccgg cttcagacgg
    37741 catgggcttg gaaagacaag gcgggaacaa aaaactgttc tgtgttgccg ctccttgctg
    37801 taccatccgt atggtttgcg gttctgccgc cctattttca aaacatgacg caggtattcc
    37861 atgtcttctt ttatttttcc cgataacggg ttcgaatggc gcaacgaaag tctgcaaaag
    37921 ccgcccaaag gttggcatta tgtccgcgaa agtggtgcaa acggcatttt gaaggctgcc
    37981 tatcaaaata ttgcaactgt ctggcagggc gatttccaca atgccaaaca ggtgctttcc
    38041 gcaatgaaga agaaggtccg caaacctgcc gccgtccgtt ccgatgcgga tatcgccgcc
    38101 cttttccacg cccaccgtat gaagcaggcg cagcagagcc gtattctgaa tatgcttgcc
    38161 gttgaaatcc gccccggttt tgtgttggac aacaaacgcg cgcccgatat acgctccgct
    38221 ttgctcgacg tgtacggaga ggcggacggc aaaccgtttt tcctgccgct caatctgctg
    38281 ctggggttta tgggtgcgca cgagtggcat aagaaagggg ttgccgttcc gcagctgggc
    38341 ggcagcatac acgttccttt cggcgtattc tcgccgttgc gcggcgaata cctcgacctg
    38401 ctcgcccatg cgccgtcaac gggttttcag acggcattcg atatcgggac gggctcgggc
    38461 gtgcttgccg ccattttggc gaaacagggc attccttccg tcatcggcac ggataccaat
    38521 ccgaaagccg tcgcctgcgc ccgtgccaat attgcccgtt tgggctttga aaaacaggtt
    38581 gagatacggg aaaccgatct gtttcccgaa gggtttgccg atctgattgt ctgcaatcca
    38641 ccctggcttc ccgccaagcc gacttccgcc gtcgaatccg cgttatacga ccccgaatct
    38701 gcgatgctgg ctgcgttttt gcgggatgcg ccgaaacatc tgaatcccga cggagaaatc
    38761 cgcctgatca tttccgatct tgccgaacat ctgcacctgc gtccgtccga ttttctggat
    38821 aaggcatttg ctcaggcggg tttgcgtgtt gccgatatga tgaaaaccaa gccgaagcac
    38881 aaaaaagccg cgaatccgag cgatccgctt gcttttgcgc gaacccggga aaccactttc
    38941 ctataccgtt tgaaaaaggc ataaggggcg gcggcgcggc attcgggcgg attattcttg
    39001 tgaaaatacc cgctcgagca tactgcccaa tgccgtctga cgcgttttga cggtggcggc
    39061 agcttgccgg aaggcttctt ccccgccgaa gaatacgaac ttctctcgcg cgcgggtaat
    39121 ggcggtatat aacagctcct tactcaatcc ggacaatgca tcgtcccctt cgtccgaagg
    39181 tgcggcggaa ggcggcagca gccatacttc ccggtattcc gaaccttggc ttttgtggac
    39241 ggtcatggcg aatgcgggtt caaattcggg caggcagctt accgctacct ttttaaatcc
    39301 gtccgcatcg gcaaaatagg cggcaaggct gccctgccgt ccgacatctt ccataatcag
    39361 tccgatgtcg ccgttgaaca gttcaagcgc gtagtcgttc tgcctgatca taatcggctc
    39421 tccggcaaaa tatgccaaat gttccggtat gttcattttg cggcgtacat ggcggcaata
    39481 ggcttcgttg aagtcttccg catcctgccg ccaagctgcc agaaccacga tatccgaaat
    39541 gcccgcgtat gcggcttcga tattgccgtc ttttaccgcc tgccaatagg ctttgtgtgc
    39601 ccggtacaac ctttcgactc gagcgttcgg actgcattcc gaatgttcca gttcgtccgg
    39661 aaaccggtca aacaatgccc acgccccttc atcgcccgat acggcggcac gggcaaggca
    39721 gccgatgccg ctgttgtcgc cgaagcggtg gctgaacgac agatgggcgg tgttttgcgc
    39781 caacacgggt ggatttgcgc tgacgctgaa accgtgttcc ggaaggaagc cggccagcct
    39841 ttggtgcgtt tctccgtcca aaacggtttt ttgtgacaaa acggacagca ccgcccctat
    39901 tccgacggac gggagctggt tttcatcccc cagcagaatc acgcgcgcgc cggttttgac
    39961 cgcttttaaa agttgcagca tcaatgccgt atccagcata gaggcttcat cgataaccaa
    40021 tacgtcaaac ggcagcgggt aaacagggtt gaacgccgcc tgcattttgg gcgggcgcag
    40081 cttcagcagt cggtggacgg tttgcccttc cagtttgagc aaatggcggc ggacggcctc
    40141 cggcgcgtca aaaccgttga ttgcacggtg cagcgcgcgc gccatatgtg ccgccgcttt
    40201 gcccgtcggt gcggcaagcg cgatgttggg aagattttcg tcttcaccgc aaatcagcgc
    40261 cagcagtttg gcaaccgttg tcgttttgcc cgttcccggc ccgccggtaa tcaccataaa
    40321 agactgcaac agtgccaagg cggcggcatc gcgctgccct tcgctgcccg tgccttgaaa
    40381 ccattttgcg aggttttgcc tcgcgcctgc cgcgtcgggg gcggatgtgc cggctgccgc
    40441 caagcgtttt atctcggcag ccaaatcgta ttccaactgc cacatcctgc ccaaaaacag
    40501 ccttctgcct tccaaaatca aaggcgcggc ggatgttccg acaacgggtg cgagtgccga
    40561 cagcgcgtca gcctcgccac cgctcaaacg gataaacgaa tgaccgtttt gcaatgcctg
    40621 aaacaggcgt tcggtgcagt ttgcaagcac ttcgtcgccg gaacccgcat agtgttccaa
    40681 aaaacggatt gccgcccttg ccgccgcttg ggcaaattca tctgtctgcc gttccatttt
    40741 attccttatc caaatgccgt ctgaaggcgt ggggcttcag acggcgcggt gttgtttcgg
    40801 ctgtttaggc gtttgcgccc tgtttggggt gcaggctgcc gtctttcatg accatcacgc
    40861 gctcgaagcg gccggcgagt tcgtcgtcgt gcgttacgac caccagcccc gttcccaatt
    40921 ctgttttcag ttccagcatc atatccaaaa cattcctggc gttcgcacga tcgagattgc
    40981 cggtcggttc gtcggcaagc aggcatttgg gttgggtaac caaggcgcgt gcaatggcgg
    41041 cacgctgccg ctcaccgcct gaaagttcgc ccgcgcggtg cgtcgaacgg tgtttcagtc
    41101 cgaccttttc cagcatcgcc attgccgcct ccgcagcctc ttcacggctt tttttgccga
    41161 tcagaagcgg catcatcaca ttttccagtg ccgaaaattc aggcagaaga tggtggaact
    41221 ggtacacgaa accgagatgg cggttacgta aatcgcccaa acgccgctgg tttaaggtac
    41281 gcaaatcctc gcccatcagc agcaccctgc cttcagacgg catatccagc ccgcccaaaa
    41341 tatgcagcag cgtcgatttg ccgctgcccg aagaaccgat gatgccggtg ctttcccctg
    41401 cgtggatttc caaatccaag ccgtgcagca cccgaacgtc caaaccgccg tcccgatagc
    41461 gtttgcccac gccttcgcat ttcaaaatca actcactcat aacgcaaagc ctccgccggt
    41521 tgggtttttg acgcgcgccg gctcgggtag agcgtggcaa cgaaagacag tcccaaagaa
    41581 atgcaggcaa tcagggcaac gtcgcccata tcgacatcgc tgggcaggta gtcgataaaa
    41641 taaacctgcg aattgatgag gtggacaccg agcaggtttt caaaaaacgc cacgaccctg
    41701 ccgacgttcc aacccaaaag cacgccgcag accacacccg ccagcgtgcc gaaaaagcct
    41761 gaaaacgcgc cctgcaccat aaaaatcttc atcacgccgc caggggaaag acccaaagtc
    41821 cgtaaaatcg caatgtccgc ctgcttttcc gtaaccgcca tcaccaagga agagacaagg
    41881 ttgaacgccg ccacggcgat aatcagcgtc aggatgatga acatcatccg tttttccaac
    41941 tcgaccgcct caaaatagct tcgattgctg aacgtccaat cgcgcaccca aaccgtatcc
    42001 ctttgcgcct ccggaatcag tgttgccgtc aaggcggggg cgttttgcgg gtcggcgagt
    42061 ttcagccgca gccccgcaac ttccttatcc aagcggtaca gtacgcgcgc gtcttggata
    42121 tgcgtcatcg ccaaggagtt gtccacttcg taaacacccg tcttaaccag accgaccacg
    42181 gtaaactgtt tcaacctcgg tacgactccg gcgggcgtaa cattgccctc cggcgtgatg
    42241 acggtaactt tattgccgac ttccgccccc aaagcctccg ccaagccgac accgaggata
    42301 atgtcaaact cgcccggaat cagatcttca aatttgcctg ccggcatttt gtcgccgtat
    42361 tccaccactt tgcgttcttc agacggcaaa atgccgcgca tctgcacgcc cctgatttcg
    42421 cccgcattgg ccagcaatgc ctgattggaa acatagggcg cggcagccaa aatacctttg
    42481 cggttttcgg taaaccgaag caggttgcgc caatccgtat ccgtattatc gatatagccg
    42541 atttcggcgt gcggcgcgac attcaggagc tgcccgcgta tttctttctg aaagccgttc
    42601 ataaccgaca agacgacaat cagcgcggtt acgcccaagg cgattccggc aatcgaaacc
    42661 atcgtgataa acgacataaa gccgttgcgc tttttcgccc tgagatacct caagcctatc
    42721 caagcctcta gagaaaacat aacgctacct taaaaatgtc tgcaaacgtg ccgccccgga
    42781 cggcggtttg ggggcggcgg caaaagttta ttgtaccgta aaacccaggc agcgtccgaa
    42841 cgcccgagtg cgggggacgg ggcggcagag cgggcggaaa acttgcacaa cgcgtcaaac
    42901 tgccctatcc tttccttcag aaaaaccgtt tcttgaggaa aacaatgaat atccgaactg
    42961 cttttgcttt gtgcgccatc gccttatccg ccgctgcggc tgcctacgcc aaagaaatca
    43021 aaatcgatgc caacaacacg ccttattccg aagccgacgc gcaaaagctg gcggcaacgg
    43081 cagtcggtat gggtgttaag gaacctatca gcctgaacgg cggcagcggc agcattaccg
    43141 tgtccggcag cagcgcgacg cagtgcgtgt tcaaagtcgg caacggaggc gcattgcaga
    43201 ttcaagggct aaactgcaag taaaccgccc ggaaaaatgc cgtctgaagg cttcagacgg
    43261 cattttgcat tggcggcgtt atgccccgcc ttctttaatc aggcggcgtt cgtacaccgc
    43321 ctgcgccagc gttcccgcat cgacatattc caattcgccg cccaagggaa tgccctgcga
    43381 cagcctgctg actttgtaag gcaggttttt aaaaaactcg gacaggacat acgccgtcgc
    43441 attgccttct gcggtaaaag cggttgcaat aatgatttct tcgacttccc cgccgcccag
    43501 ccgttgcgcc agcctgtcca atgcgatggc ggatacgtcc attcccaatg ccgtattgat
    43561 ttgccccatc aggacgaaat acagcccgtc gtggcagttt gccgcttcca tattcgacac
    43621 gtcggcaggc atatgcacca ccatcagccg ccgcccgtcg cgtgtttcat cggcacaaat
    43681 atcgcacaat ccgccttcgc aaaacgtgtt gcacatcgcg caatggtaaa cctgcttcaa
    43741 tgccgtctgc aaggcatcca ccagtttttc agcctctttg cgcttgtgtt ggagcaaatg
    43801 atacgctatc cgctgtgccg atttcggccc gacgttgggt aaaaccttca gcgcgtcgat
    43861 caatccttgg aaggcatctt gttttttgtg gctcatcata ttccgccgta tgggaaaacg
    43921 gccggaatat tccgaccgtt attttgtcaa caaaagtgtc aattactgac cgtcgccgtt
    43981 gtcgaccgat tgcgctcctt tggtctgttt gattttgccg ttgaaataac gtatcaacaa
    44041 gtcgaaagta ttggcagact gctgttgcgc caaagcctgt tttgcaagcg gaagctgtgc
    44101 ggcgatatca tccggcgggg ttacagcctg tacttcgaca atcacgggtg ccggcagacc
    44161 gatcagcctg acgtaggcgg gtttgccgtt tgccggtttt gctttcagca gttccgcata
    44221 agcctcgggc ggcatggact gccttgcctg ctgtgcgccc aaaacggaca cttccgacca
    44281 tttcacgtca acagccttgc cgccgttcag ttgggtaagc acgtcttttg ccttgttttc
    44341 ggcaagtttg gcggcttcgg tacggatata agcctgacgt accgcgtctt tggcttcggc
    44401 aaacggcagg gttttctctt cgcggacttc tttggcgcgg acgacccacg cggtttcgct
    44461 gttgatggtc agcacttcgg aattgtgttt tttcttcaat acgtcgtcgc tgaatacggc
    44521 attgatcagg ttttcgggca taccggacat ttgcgcgtcc tgcctactca gccaagtttc
    44581 ttgggtttcg actttcaaac cgctgttttt ggcggcttcg gcaagcgagg aaggatggtt
    44641 gaacgcatcg tcgcccaatt tttcttttgc cttgttgaag tcggcaaccg cctttttcat
    44701 tttcaattcg ttttcgacgg cggctttttc ctgctcgaaa gaaggtttgg cttcatttgc
    44761 cggcaaacgc gccacgcgct cttcaaacgc atttttcact tctgtttcgc tgacggtctg
    44821 tttgtctgca aagtctttca gattcaatgc gacatattcc aatttgaccg ctttgggaag
    44881 caggtagtct tttttgtttg cgttataaaa cttctgcaaa tcggcttcag acactttgac
    44941 ttgggcgatg aattcgtcgg ggttgaaagt gtgcgaacgg atggtgcggt tgacctgcgt
    45001 cagcctgatc agctgttccg cctgcgcgtc gccgaccaat acgccgtttt ggacgaggtt
    45061 taccaaattc tgcaaggcaa actgatcgcg gatttcttcg acaaactggt cttcagacat
    45121 atggcgttgc gacaggtagc ggtttaaaag cgcgtggtcg aatttgccgt ttgcgtcgtg
    45181 gaaattggga tcgtccacga taatctgctt gatttgttcg gaagaaaccg aaatgcccat
    45241 cagcttcgcg ccctgtttca ggtaggcgcg ttgtagcagg gattggaaca ccgcgtcgcg
    45301 cgaagggctg ccgccgtccg cctgctcgtt ttggacggcg gcgttaagcg actgctcgct
    45361 gattttttcg tcgcccactt ggacgatgta gtcggcgccc ggatgggata ccgtgctgac
    45421 cccgaagccg acgaaggtca atgcaatcag gcctaataag acttgggcgg gcgttctgta
    45481 tttttcgatg gaatggaaca tattttaaat cgggatatag aatgggaaat tcaagtcggg
    45541 tattgtaacg gtttttatac gcgcctgcac gaaggcttaa ctaacgtttg aatatctctc
    45601 gataagattc atcgctgaag ccgacatgaa cctttccgcc gtattccagt acgggacgtt
    45661 tgataaggct gggcatttcg gacattattc tgaccgcacc ttcctttgag gacagggctt
    45721 cctcctgcat cagtgcatta agtttgcgcc atgtagtccc ccgtttgttg agcagtatct
    45781 ccaaaggcac ctgctccagc cacaggctga tttccgcttc agacggcctc tgttttttga
    45841 aatcccgaaa ttcaaactcc aagccgtatc cggcaagcct gtttttggct tttttgaccg
    45901 tatcgcaatt tgggatgccg tgaaggacta tcatttggaa accttttgtc tgaaataata
    45961 aaacggatat tttactataa gtgtctgaaa atttgcccgt ctgtttcaga cggcggggcg
    46021 gttatgttac aatccgaaaa ttcgaaaaat ttaatctctt gttcaataaa ggctttacca
    46081 atcatgattt ctaccaacgg catcaccatg cagttcggcg cgaagccgct gtttgaaaac
    46141 gtatccgtaa aattcggaga aggcaaccgt tacggcctga tcggcgcgaa cggttcaggc
    46201 aaatccacct tcatgaaaat cctcggcggc gatttggaac agacggccgg cgaagtggcg
    46261 attgaaaacg gcgtgcgttt gggtaaattg cgccaagacc agtttgccta cgaagacatg
    46321 cgcgtgctgg acgtggtgat gatggggcat accgaaatgt gggcggcgat gaccgaacgt
    46381 gatgcgattt acgccaatcc cgaagccacc gaagacgact acatgaaagc cgccgaactg
    46441 gaagccaagt tcgccgaata cgacggctac accgccgaag cgcgtgccgc cgaactgttg
    46501 agcggcgtgg gcatttccga agatttgcac aatgcgaaaa tggcggaagt cgccccgggc
    46561 ttcaaactgc gcgtattgct ggcgcaggcg ctgttctcca agccggatgt attgctcttg
    46621 gacgaaccga ccaataactt ggacattaat accatccgct ggttggaagg cgtgttgaac
    46681 caatacgact ccacgatgat tatcatcagc cacgaccgcc actttttgaa cgaagtctgc
    46741 acgcatatgg cggatttgga ctacaacacc atcaccatct atccgggcaa ctacgacgat
    46801 tacatgctcg cttctgccca atcgcgtgag cgcgccctga aagacaacgc caaagccaaa
    46861 gagaagctgc aagagctgca agagtttgtc gcacgcttct ctgccaacaa atccaaagcc
    46921 cgtcaggcaa ccagccgtct gaaacaggcc gacaaaatca aatcggagat ggtcgaagtc
    46981 aaaccttcca cccgtcaaaa cccgtatatc cgttttgaag ccgatgaaaa agccaagctg
    47041 caccgtcagg ctgtggaagt tgaaaaactg gcgaaacgct ttgaaaccca gttgtttaaa
    47101 aacctgaact tcatccttga agcgggacaa cgcctcgcca tcatcggccc gaacggcgcg
    47161 ggcaaatcca ccctgctgaa actcttggcc ggcgcgtaca accccgaata ttcagacggt
    47221 ctgctgccgg acgaaggcag catcaaatgg gcggaaaaag ccagcgtcgg ctactatccg
    47281 caagatcacg aaaacgactt cgatgtcgat atggatttga gcgaatggat gcgccaatgg
    47341 gggcaggacg gcgacgacga acaagtcatc cgcggcactt tggggcgttt gctcttcggc
    47401 agtaacgatg tcgtgaaaaa agtgaaggtt ctctccggtg gtgaaaaagg ccgtatgctt
    47461 tacggcaaac tgttgctgtt gaaacccaat gtcttggtca tggacgaacc gaccaaccac
    47521 atggacatgg aaagcatcga atccttgaac atggcgctgg aaaaatacaa cggcacgctg
    47581 attttcgtct cccacgaccg tcagttcgtt tcttccctgg ctacccaaat cattgaattg
    47641 gacggcaaag gcggatatga acactacttg ggcgattacg aaagctatct cgagaaaaaa
    47701 ggcgtagcat aaccgccggt tggaacaatg ccgtctgaag cggcttcaga cggcattgtt
    47761 gataacttta aaataggaag catatgcaga cttatctcgt cggcggtgcc gtccgcgatt
    47821 atcttttggg cttgcccgtc aaagaccgcg attgggtggt cgtcggcgca gacgcacaaa
    47881 ccatgctggc gcaaggcttc cagccagtcg gcaaagactt tcccgtgttt ctccatcccg
    47941 aaacacacga agaatacgcc ctcgcccgca ccgagcgcaa aaccgccaaa ggttacgccg
    48001 gcttcagttt ccacaccgac aaagacgtta cgctggagca ggatttgatg cgccgcgacc
    48061 tgaccatcaa cgcgatggcg caagatgcgg acggcaagat tatcgaccct ttcggcggac
    48121 aacgggattt ggcggcaggc attttgcgcc acgtttcccc ggccttcgcc gaagaccccg
    48181 tccgcatcct gcgtactgcc cgctttgccg cgcgttacaa gtttgaaatc gccgaagaaa
    48241 ccataaagct gatgcggcag atggtggaaa acggcgaagc ggacgcattg gttgccgaac
    48301 gcgtctggca ggagtttgcg aaaggtttga tggaaaaaaa tccgcgcaaa atgattgaag
    48361 tgttgcgcga atgcggcgcg ctcaaagtct tgctgcccga agtcaatgcc ctcttcggcg
    48421 tgccgcaacg cgccgactac catcccgaaa tcgacagcgg catccatacc ctgatgacgc
    48481 tgcaacgcgc cgccgatatg ggtttgagcc tgcccgaacg ctatgccgcc ctgctgcacg
    48541 acttgggcaa agccaaaaca ccgtccgaca tcctgccgcg ccaccacggg cacgacctcg
    48601 ccggtgtcga acccgtgcgc gaagtcaatc agcggctgcg cgcgccgaaa cattgcgccg
    48661 agcttgccga attggtttgc cgttggcaca tcatcttcca tcaggttgaa aaactccgca
    48721 gcaaaaccat tatcgacgta ttgaaaaaaa ccgatgcctt ccgccgccca gaacgttttc
    48781 agacggcatt gaacgtctgc attgccgaca cgcaaggccg tctgaaccgc gaacacacgc
    48841 cctacccgca acgcgcgcac tggctcgcct tactcgaagc cgccaatcag gcggattcgg
    48901 gcaaaatcgc cgccgaatgc cgcgcgcagg gaaaagcgca ccttatcgcc gaacaaatcg
    48961 accgggcgcg gctggcacaa atcgccccat tgcaaaaagc gtttcgagcg gcgcaagaca
    49021 aaacagaaaa acattaaaac gtccaatgca gccactttta tagtggatta acaaaggggc
    49081 ttaggaagca aaatcatcaa aaaacctgat aagctcctaa tatgaaacta aaaaataagt
    49141 atcaaaagtt cagcaaaatt tcagagcaaa agtttagaga aataatccgc tgtttcgctc
    49201 ttgatttgac cgcttccgat actgctaaaa tgaccggcat tagtgtacgc ggtatcaatc
    49261 ctattttcct gaaaatcaga cacaggattg ctgctctgtg cgaacaaagc tcaccactgt
    49321 ccggtgtggt cgaattagat gaatcttact ttggtgcgcg acgtatcagg ggtaagcgcg
    49381 gtcgcggagc gtcaggtaaa accatcgtat ttggcatact gaaacgcaat ggcgtggtct
    49441 atacggaaat cgttcccgac gcttcgaaag cctcactaat caaggtcata cgtggtcaca
    49501 tatctgctga cagtgaaatc aatactgacg gctggaaagc atatgacggt cttgtcgata
    49561 tgggctatga gaagcattac cgtgtccatc atggttccaa tgagtttgct cgaggaaagc
    49621 aacatatcaa tggtattgaa tctttttgga gttatactaa ggggcgtttg gctaaatttc
    49681 atggtatttc caaagagatg ttttatctgc atctcaaaga aacggagttt aggtttaacc
    49741 accggcatga agatttaggt aaaatattaa tgaagatgct tcgaaataac ccaatttaat
    49801 atttttgctt cctaagcccc taa
//
LOCUS       DAXHNE010000002        39892 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.002, whole genome shotgun sequence.
ACCESSION   DAXHNE010000002 DAXHNE010000000
VERSION     DAXHNE010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 39892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39892
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.002"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(23..714)
                     /locus_tag="ACFM4Z_000048"
                     /pseudo
     CDS             complement(23..714)
                     /locus_tag="ACFM4Z_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(1020..1790)
                     /locus_tag="ACFM4Z_000049"
     CDS             complement(1020..1790)
                     /locus_tag="ACFM4Z_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020167.1"
                     /translation="MASIALYRLSFPVAAGGSGSRCTLCTKAHRESMLAIYSINILIY
                     NEKSFFRVTLYFVKNFVSFSKPRKSNEMMQKHKCLIDFILTVASQIAYADLPLSLEEL
                     LTDKGKFKLESSISYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQV
                     FDSQNVKAIQLSQAEMKETQGEFVPIIAAAAFGGALGAWGYHGANLYNHGKLGTAQGA
                     ATATGIGAATGVAAKQGWLLLPEADWLGIWHGDRVFMH"
     gene            complement(1821..1979)
                     /locus_tag="ACFM4Z_000050"
     CDS             complement(1821..1979)
                     /locus_tag="ACFM4Z_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020168.1"
                     /translation="MKIPLPICTLSALAACTLSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(1976..2818)
                     /locus_tag="ACFM4Z_000051"
     CDS             complement(1976..2818)
                     /locus_tag="ACFM4Z_000051"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6020169.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPILVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3057..3977
                     /gene="pyrB"
                     /locus_tag="ACFM4Z_000052"
     CDS             3057..3977
                     /gene="pyrB"
                     /locus_tag="ACFM4Z_000052"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFC6020170.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWNCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLARAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            3987..4445
                     /gene="pyrI"
                     /locus_tag="ACFM4Z_000053"
     CDS             3987..4445
                     /gene="pyrI"
                     /locus_tag="ACFM4Z_000053"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFC6020171.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4510..5175
                     /locus_tag="ACFM4Z_000054"
     CDS             4510..5175
                     /locus_tag="ACFM4Z_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020172.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQILAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5238..6455)
                     /locus_tag="ACFM4Z_000055"
     CDS             complement(5238..6455)
                     /locus_tag="ACFM4Z_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC6020173.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            6639..7142
                     /gene="def"
                     /locus_tag="ACFM4Z_000056"
     CDS             6639..7142
                     /gene="def"
                     /locus_tag="ACFM4Z_000056"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFC6020174.1"
                     /translation="MALLNILQYPDERLHTVAKPVEQVDERIRKLIADMFETMYESRG
                     IGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYEEGCLSVPGIYDTVT
                     RAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVERLSQLKQGRIKTKLK
                     KRQKHTI"
     gene            7229..8155
                     /gene="fmt"
                     /locus_tag="ACFM4Z_000057"
     CDS             7229..8155
                     /gene="fmt"
                     /locus_tag="ACFM4Z_000057"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFC6020175.1"
                     /translation="MKVIFAGTPDFAAAALRAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVEQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSKS
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGAKL"
     gene            8234..9493
                     /gene="rsmB"
                     /locus_tag="ACFM4Z_000058"
     CDS             8234..9493
                     /gene="rsmB"
                     /locus_tag="ACFM4Z_000058"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFC6020176.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLMAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIGNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLRERDKLVASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLVAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASTACADAQDLSAWYDGKPFDAVLADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDEQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            9459..10058
                     /locus_tag="ACFM4Z_000059"
     CDS             9459..10058
                     /locus_tag="ACFM4Z_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HFC6020177.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10058..12178
                     /locus_tag="ACFM4Z_000060"
     CDS             10058..12178
                     /locus_tag="ACFM4Z_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6020178.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAIPVQIDLIGAASLPGDMGRVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHKLDQPFPGKARWEKIQRAGSVRDLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPIPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATL
                     LIASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGRLKTFNKAAE
                     QILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSSSLKLENQDLNALI
                     GDVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVK
                     SEAGRDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRI
                     SLSNQDAGGACVRIILPKTVETYA"
     gene            12171..13448
                     /locus_tag="ACFM4Z_000061"
     CDS             12171..13448
                     /locus_tag="ACFM4Z_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFC6020179.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSMVENALKYGAAQTETGPVFDKLGNSAAIQEMNCEVEAAAKRTSPVF
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAEHRRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPASFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAGAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            13539..14726
                     /gene="dprA"
                     /locus_tag="ACFM4Z_000062"
     CDS             13539..14726
                     /gene="dprA"
                     /locus_tag="ACFM4Z_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFC6020180.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLLHHFGSAQNALSAPAEQV
                     AALIRHKQALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLAQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRYATPQAMRIAKDFGRALGGKGIPVVSGMASGIDT
                     AAHQGALQAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEA
                     AAGGTAHGGILDKMGFDPVHPDVLAEQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            14752..15213
                     /locus_tag="ACFM4Z_000063"
     CDS             14752..15213
                     /locus_tag="ACFM4Z_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HFC6020181.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            15285..17591
                     /gene="topA"
                     /locus_tag="ACFM4Z_000064"
     CDS             15285..17591
                     /gene="topA"
                     /locus_tag="ACFM4Z_000064"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFC6020182.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(17664..18359)
                     /locus_tag="ACFM4Z_000065"
     CDS             complement(17664..18359)
                     /locus_tag="ACFM4Z_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020183.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFAPEMN
                     RILSDFKTTRGFK"
     gene            complement(18379..18930)
                     /locus_tag="ACFM4Z_000066"
     CDS             complement(18379..18930)
                     /locus_tag="ACFM4Z_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020184.1"
                     /translation="MEFLSLYPWWSFFIILTIILSYIGFCAHLHIQNRAVFFYSYRDV
                     TIIFLTPVILIALSYLIKNKEILSACILCITLIAFSWAWIITYRSNTKRFFLSLLIVW
                     GKLLLSTIVWAILAISLGLAVITGNSKRKKYERRDRHAERNEKAFIGALLVGFELSRN
                     LLNLCCLHKDFSTPSKNIEVNRS"
     gene            19048..19425
                     /locus_tag="ACFM4Z_000067"
     CDS             19048..19425
                     /locus_tag="ACFM4Z_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFC6020185.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            19514..20083
                     /gene="rsmD"
                     /locus_tag="ACFM4Z_000068"
     CDS             19514..20083
                     /gene="rsmD"
                     /locus_tag="ACFM4Z_000068"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFC6020186.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGMEAASRHAKRIVIADNNRQTVQTLEKNSRELGLG
                     QVQIVCSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PDIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            20206..20457
                     /locus_tag="ACFM4Z_000069"
     CDS             20206..20457
                     /locus_tag="ACFM4Z_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HFC6020187.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            20554..20637
                     /locus_tag="ACFM4Z_000070"
     tRNA            20554..20637
                     /locus_tag="ACFM4Z_000070"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20588..20590,aa:Tyr,seq:gta)
     gene            20668..20741
                     /locus_tag="ACFM4Z_000071"
     tRNA            20668..20741
                     /locus_tag="ACFM4Z_000071"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20700..20702,aa:Gly,seq:tcc)
     gene            20751..20825
                     /locus_tag="ACFM4Z_000072"
     tRNA            20751..20825
                     /locus_tag="ACFM4Z_000072"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20783..20785,aa:Thr,seq:ggt)
     gene            20874..22058
                     /gene="tuf"
                     /locus_tag="ACFM4Z_000073"
     CDS             20874..22058
                     /gene="tuf"
                     /locus_tag="ACFM4Z_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC6020188.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSS
                     YDFPGDDCPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVG
                     VLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFR
                     TTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSV
                     IA"
     gene            22065..22140
                     /locus_tag="ACFM4Z_000074"
     tRNA            22065..22140
                     /locus_tag="ACFM4Z_000074"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22098..22100,aa:Trp,seq:cca)
     gene            22255..22533
                     /gene="secE"
                     /locus_tag="ACFM4Z_000075"
     CDS             22255..22533
                     /gene="secE"
                     /locus_tag="ACFM4Z_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFC6020189.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            22535..23071
                     /gene="nusG"
                     /locus_tag="ACFM4Z_000076"
     CDS             22535..23071
                     /gene="nusG"
                     /locus_tag="ACFM4Z_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFC6020190.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            23172..23606
                     /gene="rplK"
                     /locus_tag="ACFM4Z_000077"
     CDS             23172..23606
                     /gene="rplK"
                     /locus_tag="ACFM4Z_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFC6020191.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKDPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            23606..24301
                     /gene="rplA"
                     /locus_tag="ACFM4Z_000078"
     CDS             23606..24301
                     /gene="rplA"
                     /locus_tag="ACFM4Z_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFC6020192.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            24531..25031
                     /gene="rplJ"
                     /locus_tag="ACFM4Z_000079"
     CDS             24531..25031
                     /gene="rplJ"
                     /locus_tag="ACFM4Z_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFC6020193.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            25089..25460
                     /gene="rplL"
                     /locus_tag="ACFM4Z_000080"
     CDS             25089..25460
                     /gene="rplL"
                     /locus_tag="ACFM4Z_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFC6020194.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            25650..29828
                     /gene="rpoB"
                     /locus_tag="ACFM4Z_000081"
     CDS             25650..29828
                     /gene="rpoB"
                     /locus_tag="ACFM4Z_000081"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFC6020195.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAAEKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNKLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            29985..34160
                     /gene="rpoC"
                     /locus_tag="ACFM4Z_000082"
     CDS             29985..34160
                     /gene="rpoC"
                     /locus_tag="ACFM4Z_000082"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFC6020196.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIITDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            34452..34709
                     /locus_tag="ACFM4Z_000083"
     CDS             34452..34709
                     /locus_tag="ACFM4Z_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020197.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRMGMPINIG"
     gene            34706..34855
                     /locus_tag="ACFM4Z_000084"
     CDS             34706..34855
                     /locus_tag="ACFM4Z_000084"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6020198.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSGKERFVS
                     IEKTY"
     gene            35034..35405
                     /gene="rpsL"
                     /locus_tag="ACFM4Z_000085"
     CDS             35034..35405
                     /gene="rpsL"
                     /locus_tag="ACFM4Z_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFC6020199.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            35523..35993
                     /gene="rpsG"
                     /locus_tag="ACFM4Z_000086"
     CDS             35523..35993
                     /gene="rpsG"
                     /locus_tag="ACFM4Z_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFC6020200.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            36012..38117
                     /gene="fusA"
                     /locus_tag="ACFM4Z_000087"
     CDS             36012..38117
                     /gene="fusA"
                     /locus_tag="ACFM4Z_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFC6020201.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEVDKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            38203..39387
                     /gene="tuf"
                     /locus_tag="ACFM4Z_000088"
     CDS             38203..39387
                     /gene="tuf"
                     /locus_tag="ACFM4Z_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC6020202.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILSKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSS
                     YDFPGDDCPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVG
                     VLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFR
                     TTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSV
                     IA"
     gene            39405..39716
                     /gene="rpsJ"
                     /locus_tag="ACFM4Z_000089"
     CDS             39405..39716
                     /gene="rpsJ"
                     /locus_tag="ACFM4Z_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFC6020203.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
BASE COUNT        10310 a         9671 c        10574 g         9337 t
ORIGIN      
        1 taaatgggca aaaatcggtt tattatcgtt tttgccgcat ttggatttgt tctaccgtaa
       61 aacgtgtttg acgaacggga ttcttattaa aaaacatctg atttctaaca aaatcagtat
      121 tttttggcac gatggctaaa atttttcctt ccatttcgcc atcacgtgtt ttccatgcgc
      181 tcaagaattg tgatttgctc attgagacgt gccccagcga tggatcagcc agcaaaacag
      241 tttctccgtt aataccgttc aataccgaaa aatggttgtt tttacggtat tttaaataca
      301 caattacagg aatttttagt tgtaccaact gttcaaatgg caaagcataa ccttgtgctt
      361 caaaacccag ttcgggcatt atgcgttgca tatcgtcaaa agaagcacgc atttgggttt
      421 tatccatttt gtctaagatt tccgcttcag aataatgtct gccataaaaa ttattcagta
      481 acgtggcaat cgaagccgcg ccgcaagaaa aatccaaatc ttgttttact atgccggaat
      541 ctcgccgtgc tttccaactc cgtacatgga tgttttggta agaagcgggg ggtcaacaaa
      601 cataggccaa gcaaaaacta tatttggggc gaaaccaatc aaagccgcat aatttatcaa
      661 tttataaaga ttttttatca taatatgtat acgcggaata aacgcatgaa acataaaaaa
      721 ataaaatcat atgcaatttt attgcatgaa taaatatgaa taaaatagat aatgatggga
      781 gtaaatacgc catgtatttt ggaagtttaa atttattaat aataaaataa tttatcgtag
      841 cgcaaataaa tcccaaaatt ggaacgatta gaaaaaaaat taatacaccc atcatcatgc
      901 aaactctata ttaataaata gctaactaat tctattgtgg aaattagtta gctaactaaa
      961 agttattaat gattattttc gagaattgac tgcattgttg gcagcattgg caccaaaacc
     1021 tagtgcatga atacccggtc tccatgccaa attcccagcc aatccgcctc cggcagcagc
     1081 agccagccct gttttgccgc tactcctgtt gccgcaccga ttcctgtcgc agtagccgcg
     1141 ccttgcgcag ttcctaattt accatgatta tacaaattag caccatgata cccccatgca
     1201 cctaatgcac cgccaaaagc agcggctgca ataatgggaa caaattcacc ttgtgtttct
     1261 ttcatttcag cctgtgataa ttgaattgct ttcacatttt ggctgtcaaa aacttggctg
     1321 tctaaatttt gcgccattac aggtgtaatc atcatagcca ttacagttgc aattttcgtt
     1381 gcgctggttt gcacataaat aggattagca aattcgcttt gattgcgttc agtgttgatg
     1441 tagctaatac tgctttctag tttgaattta cccttgtcag tcaataattc ttccaaactt
     1501 aaaggtaagt cagcataagc aatttggctg gcaacagtca aaataaaatc tattagacat
     1561 ttgtgttttt gcatcatttc gtttgatttt ctaggttttg agaatgatac aaagtttttt
     1621 acaaagtaaa gagtcactct gaaaaaactt ttttcattat aaatcaaaat attgatagaa
     1681 taaatagcga gcatcgattc acggtgcgct ttagtgcaaa gcgtacaacg cgagcctgaa
     1741 ccaccagccg caacaggaaa agaaagccga tagagtgcaa tgcttgccaa cgtgcaagcg
     1801 agcttgcaag aacgcttggc tcaacgagag caggcaagac agaaagcaga aagcaggata
     1861 ggagcggtaa cgcaaaggtc tcgggctttg atttcgccgt aaaccctgct gccgccttgt
     1921 ccggaaaggg tgcaggcggc gagtgccgac agggtgcaga tggggagggg gattttcatt
     1981 tggggtcgca acggaagtgg tatgcgcaga tttcaaaacc gtttttgaaa tacaggcggt
     2041 gcgcgtcggc acggtcgtgg ttgacgtgga cgttgaggtg gattttggtt acccctgttt
     2101 ccgcgccgat tttgcggact tcttccaaaa ggcgcgaggc gtagcctttg cggcggcttt
     2161 gcggcagggt aacgatgtca tcgatgtgga tgtggcggcc gctggcgagg gtgcaggctt
     2221 cgcggaagcc gcagacggcg acggcattgt gtttgccttc ttcaaaaata cccagcaggc
     2281 ggtagccttg ggggcgttgg actttgttga tctgttcggt aaagcggttg atgtcggtca
     2341 gggcggaacg caaaacgctc aaggctgcaa aggcggtggc ggtgtcgtcc gcgccgattt
     2401 cgcgcaaaac gtaggatgcg cccgaggcgg tctgttcctg tgctttctcg gcggcgtgtt
     2461 tttcttcgat tgcctgtgcc agcatgacgt gttcgtcggc agggttgttt tgtccgccct
     2521 gttcgcgttc ttcgagcagg gctttgcagt cgatgacgcg caggtcgttg tcggcggcaa
     2581 agtccatcag gaagcggaac atttggggat tgtcggtttc cagtttttta tcgacggcga
     2641 cgcagcggat gttgtccacc agtatggggc gcacccattt cgaataggaa agcctgtggt
     2701 ctttggtgaa cgaggacaga atgccgcaca ggcgttccgc ccagtcgctg ggacggaaaa
     2761 tcttgccgga actcgttgtg ccgtggatga cgacttcgta ggggttgcag actaacatgg
     2821 cggcttcctg aaaagaaatg tctagcgcga ttatacctta tgcttatgcg ggcgtgtttg
     2881 gatatgccgt ctgaaaagta cgggattcgt gcggtaaaac tttgcggcgg caaatgtgcg
     2941 ataatacgcg ccgtattgcc gcttttgcga agctgttccg caaacatacg ggcggcgtgg
     3001 acgacgtata accggatacc cgcctgacgc gggtttttta cggaaggggg gcaaaaatgc
     3061 ctaatccgct ttacagacag catatcatct ccatttcgga tttgtcgcgc gaacagttgg
     3121 aatgcctgct tcagacggca ttgaagctga aggcgcatcc gcgcggcgac ctgttggaag
     3181 gcaaacttat cggttcgtgc tttttcgagc cgtccacgcg cacgaggctg tcgtttgaaa
     3241 cggcggtgca gcgtttgggc ggcaaggtca tcggtttctc ggacggcgcg aataccagtg
     3301 ccaaaaaagg cgagacgctt gccgataccg cccgcatcat ttccggatat actgatgcta
     3361 tcatccaacg ccaccccaaa gacggcgcgg cgcgcgtggc agcggagttt tcgcgcgtcc
     3421 ccgttatcaa cgccggcgac ggcacgaacc agcaccccag tcagacgctg ctcgacctgg
     3481 ttaccattta tgaaacacag ggacgtttgg acaagctcaa aatcgccatg gcgggcgact
     3541 tgaaatacgg acgtaccgtg cattcgcttt gtcaggcgtt gaaacgctgg aattgtgaat
     3601 ttgcctttgt ttcgccgccc agcctagcca tgcccgacta tattaccgaa gagttggacg
     3661 aagccggctg ccgataccgt atcctcggta gtttggaaga agcggcggaa tgggcggata
     3721 tcctgtatat gacccgcgtc cagcgcgaac gtttcgacga acaggaattt gccaaaatcc
     3781 aaggcaaatt caacctcgaa gcgtctatgc tcgcccgcgc caaaccgaac ctgcgcgtgc
     3841 tgcaccccct gccgcgcgtg gacgaaatcc atcccgatgt cgatgccacg ccgcacgcct
     3901 attatttcga gcaggcgacc aacggcgttt atgcgcgtat ggcgatattg tcgctggtgt
     3961 tgaacgaaga agtgtgagga accgatatgg aaaccccgaa actcagtgtc gaagccattg
     4021 aaaaaggtac ggttatcgac catattcccg ccggcagggg gctgaccatc ctgcgccagt
     4081 tcaaactttt gcactacggc aacgcggtaa ccgtgggctt caacctgccc agcaaaaccc
     4141 aaggcagcaa agacatcatc aaaatcaaag gcgtgtgctt ggacgacaaa gccgccgacc
     4201 gcctcgccct gttcgccccc gaagcggtgg tcaacaccat cgacaatttc aaggtcgtgc
     4261 agaagcggca tttgaacctg cccgacgaaa tcgccgaagt gttccgctgt ccgaacacga
     4321 attgcgccgg ccacggcgag ccggtcaaaa gccggtttta tgttaaaaag cacaacgggc
     4381 agacgcggct gaaatgccac tactgcgaaa aaacctacag ccgggattcg gtggcggaag
     4441 cctgacggat tcccttaaac cgagtgggcg gcatttcgtc tgccgcctgt tttgccaatc
     4501 tgaaatggaa tgatgatgca cgcttctgtc caaagccgtt tcgcaccgat actttatgtt
     4561 ttgattttct ttgccggttt tttgaccgcg caaatctggt tcaatcagaa agcctatact
     4621 gaagagctgc ctccgcttct gtccgcattg tccgccgtcg cgctggtgtg gctggcgtgg
     4681 gcgttcgtgt cggcgcgttc aaaggccaag gcggaaaagt tctaccgcga aaaaatgata
     4741 cagaacgaaa gcatacaccc cgtcctgcac gcctctttgc aacacttgga acacaagccg
     4801 caaatactcg ccctgctggt caaaaaccac ggcaaaggga tggcggaaca ggtcaggttc
     4861 aaggcggaag tgctgcccga cgacgaagac gcgcgcacga ttgccgccga gttggcaaaa
     4921 atggatatgt tcgcattggg gacggacgcg gtcgcctcgg gcgaaaccta tggacgcgtg
     4981 ttcgccgata ttttcgagtt gtcggcggct ttggaagggc gcgcgttcaa aggaatgttg
     5041 aaactgacgg cggaatataa aaacatcttc ggcgatgcct gccgttcgga aacggcgttg
     5101 gagttgggcg cactcaatca ggcgttgcag gagatttcaa aaacatcgga aaagtccaaa
     5161 cggatatttt attgaagatg gaaaaatgcc gtctgaaacg gaaggtgttt cagacggcat
     5221 ttttgtcgga tgattaatta ttcggagcgg ttgaagccaa acttcacgcg gctgcggccc
     5281 tgatccggta tattgtccaa atcgcgtccc ggattggcgg cggtgtcgcc tacggaaata
     5341 tcggagatgt tttccaaaat gatggcggac gacaggtgtt cggaggtgcg gtaaaccatt
     5401 gccaagccca cttcttcggc aggagtggaa atcagctcga cggtatccct gcttttgaaa
     5461 ttgttggaga ggtcgacctg catcgttttc ttgcgtttgt agaggctcaa aaccgtgcct
     5521 ttgtccaaac cgtccgcctc gcctttgtcg atggtgatgg tttgaaactg gccggcaatc
     5581 cttgtgcctt caaacacgga aacgatttta gcctgaaccg ggcgggacgg ttcgtgcggc
     5641 atcatgttga agcggtcggt gtcttccggc attttcatca ggtagtcgcc ctgctgtatt
     5701 tcggaaatgg cggtttcgac caccagcggc tgtatcgaag gggtgcgcag cggggtaatc
     5761 aaaggatggg tgcgggtatg gtattcgttg tctttcggcc gttctccagc ctgtttcgag
     5821 cgttgttcga ggacagagtc ggtatagtcg agggagcgca cgatgccgct gaatgcgact
     5881 tcctgcccga ggaatttacc cgtatccgga tcggtgatgt ttttattgat tcggtaggtc
     5941 aggtagcggc ccggctcttt caggcctttg gtgtaaaccc tggtgccttt ggtgtacagc
     6001 agcctgcctt ccgggcccga gagcaggcgc ggcgcggcag cggtttcttt gcgggaaacg
     6061 atttgcggat gccgcataaa gatgcggtag aagttgacat cgatggcggg aataccgtat
     6121 ccggacactt ccttatccgg actcattttg acgacgggga tgccgtctgt ctgttccaag
     6181 ccgaggcgcg gttcgccgtc aacgtggcgc aacaccaata cctggtccgg ataaatcagg
     6241 tcgggattgt ggatttgatc ccggttcgcg tcccacaggc ggccccattg ccacgggctg
     6301 tacaggtatt tgcccgaaat gccccacagg gtgtcgccct gtttgaccgt gtagcgttcc
     6361 ggcgcgttcg ggcgcacctc caaatttgcc gccaaagttt gtgttgagaa tgccatacct
     6421 gccgcgcaga gcagggttat aatacgacgt tgcataaccg ttccccttat ctgataaatt
     6481 tcggtttgtc ttgcttgatt gggttggaaa aagcggcggc agcccctcgg gatgtgccgc
     6541 gtgataaaaa atgttccgca ttttaacatc gaattatccg caccatcacg gtaattatga
     6601 aaaacaggcg gcgtatccgc cgaaggaaag agaaaattat ggctttattg aatatcttgc
     6661 aatatcccga cgagcgtctg cacacggtgg caaagcctgt cgaacaagtc gacgagcgca
     6721 tccggaagct gattgccgat atgtttgaaa cgatgtacga atcgcgcggc atcgggctgg
     6781 cggcgacgca ggtcgatgtg cacgagcgcg tggtcgtgat ggatttgacc gaagaccgca
     6841 gcgaaccgcg cgtgttcatc aaccccgtca tcgttgaaaa agacggcgaa accacttacg
     6901 aagagggctg cctgtccgtg ccgggcattt acgacaccgt aacccgcgcc gaacgcgtca
     6961 aggtcgaggc tttgaacgaa aaaggcgaaa agttcacgct ggaggcggac ggcttgttgg
     7021 cgatttgcgt gcagcacgag ttggaccacc tgatgggcat cgtgtttgtc gaacgccttt
     7081 cccaactcaa gcaggggcgg attaagacca agctgaaaaa acgtcagaaa catacgattt
     7141 gacccttttg ccgtgccgtc tgaacgctgc aaagttttca gacggcacgg tcttgtccga
     7201 caattttacg cacgcgcagg aacacgctat gaaagtcatc ttcgccggca cgcccgattt
     7261 tgccgccgcc gccttaagag ccgttgccgc cgccggtttt gaaattccgc tggtgctgac
     7321 ccagcccgac cgtccgaaag ggcgcggtat gcaactgact gccccgcccg tcaaacaagc
     7381 cgcgctggaa ctcggtttgc gcgtcgaaca gcccgaaaag ctgcgcaaca acgccgaagc
     7441 cctgcaaatg ctcaaagagg tcgaggcaga cgtaatggtg gttgccgcct acggtttgat
     7501 tctgccgcag gaagtgttgg atacgccgaa acacggctgc ctcaacatcc acgcttcgct
     7561 gttaccccgt tggcgtggcg cggcgccgat tcaacgcgcg attgaagccg gcgatgccga
     7621 gacaggcgtg tgtattatgc agatggacat cggtttggac accggcgatg tggtcagcga
     7681 acaccgctac gccatccaac cgaccgatac cgccaacgaa gtccacgacg cgctgatgga
     7741 aatcggtgcg gcggcggttg ttgccgattt gcaacagctt caaagcaaag gccgtctgaa
     7801 cgcggtcaaa cagcccgaag aaggtgttac ttacgcgcaa aaattgagca aagaagaggc
     7861 gcgtatcgat tggagcaaaa gcgcggcggt tatcgaacgc aaaatccgcg ccttcaaccc
     7921 cgtgcctgcc gcgtgggttg agtatcaggg caagccgatg aaaatccggc gggcggaagt
     7981 ggtggcgcaa caaggcgcgg caggcgaagt gttgtcctgt tcggcggacg gtttggtcgt
     8041 tgcctgcggc gaaaacgcgc tgaagattac cgaattgcag cctgccggcg gcaggcggat
     8101 gaatatcgcg gcgtttgcag caggacggca tatcgaagca ggggcgaagc tgtaaatccc
     8161 ttcagacggc attccgatcc gcaaacggga atgccgtctg aaaccatcag tcgaagaaag
     8221 cgaatcacat aatatgagta tggcacttgc ccaaaaactt gccgccgaca gcattgcggc
     8281 ggttgccgaa ggacgtaacc ttcaggacgt gttggcgcaa atccgcaccg cgcatcccga
     8341 ccttatggcg caggaaaacg gcgcgttgca ggacatcgcc tacggctgcc agcgttattt
     8401 gggcagtttg aaacatatgc tcgcgcagat gctgaaaaag ccgattggca atccgcagct
     8461 cgaaagcctg cttttggcgg cgttgtacca gctgcattac acgcgcaacg cgccccacgc
     8521 cgtggtcaat gaggcggtgg aaagcatcgc gaaaatcgga cgcgggcagt accgttcgtt
     8581 tgccaacgcg gttttgcgcc gctttttgcg cgaacgcgac aagcttgtgg cttcctgtaa
     8641 aaaagacgat gtagcgaaac acaacctgcc gctgtggtgg gtggcttact tgaaaaacca
     8701 ttatccgaaa cactggcaca acatcgccgc cgcgctgcaa tcccatccgc cgatgacttt
     8761 gcgcgtcaac cgccgacacg gcaatgccga aagctatttg gaaaaactgg tggcggaagg
     8821 tatcgcggct aaggcgttgg acgaatatgc ggttacgttg gaagaagccg tgccggtgaa
     8881 ccgcctgcct ggtttttcag acggcattgt ttcggtacag gacttcggcg cgcagcaggc
     8941 ggcgtatttg ttaaacccga aagacggcga acggattttg gacgcgtgcg ccgcgccggg
     9001 cggcaagacg gggcatatct tggaactggc ggattgccgt gttaccgcct tggacattga
     9061 tgcaggccgt ctgaaacggg tggaagacaa tatcgcgcgt ctgggctttc agacggcatc
     9121 gacggcgtgt gccgatgcac aggacctgtc ggcatggtat gatgggaaac cgtttgatgc
     9181 cgtccttgcc gacgtgccgt gtaccgcttc cggcgtggcg cggcgcaatc ccgacgtgaa
     9241 atggctgcgc cgtccgaccg acgcgctcaa aaccgcccgc cagcaggaag ccctgctgga
     9301 cgcactttgg cagacgctga aacagggcgg cagaatgctg cttgccacct gttccgtgtt
     9361 cgtcgaagaa aacgacgagc aattgcaaaa attcctcaac cgccatgccg atgccgaacc
     9421 gatcgaatcg cgggtgcttt taccgaacaa acaccaagat ggcttttatt acgcgcttat
     9481 tcaaaagcat taaacaatgg cttgtgctgc tgccgatgct ttccgttttg ccggacgcgg
     9541 cggcggaggg gatagatgtg agccgcgccg aagcgaggat aaccgacggc gggcagcttt
     9601 ccatcagcag ccgcttccaa accgagctgc ccgaccagct ccaacaggcg ttgcgccggg
     9661 gcgtgccgct caactttacc ttaagctggc agctttccgc cccgataatc gcttcttatc
     9721 ggtttaaatt ggggcaactg attggcgatg acgacaatat tgactacaaa ctgagtttcc
     9781 atccgctgac caaccgctac cgcgttaccg tcggcgcgtt ttcgacagac tacgacacct
     9841 tggatgcggc attgcgcgcg accggcgcgg ttgccaactg gaaagtcctg aacaaaggcg
     9901 cgctgtccgg tgcggaagca ggggaaacca aggcggaaat ccgcctgacg ctgtccactt
     9961 caaaactgcc caagcctttt caaatcaatg cattgacttc tcaaaactgg catttggatt
    10021 cgggttggaa acctctaaac atcatcggga acaaataatg cgccgttttc taccgatcgc
    10081 agccatatgc gccgtcgtcc tgttgtacgg actgacggcg gcaaccggca gcaccagttc
    10141 gctggcggat tatttctggt ggatagttgc gttcagcgca atgctgctgc tggtgttgtc
    10201 cgccgttttg gcacgttatg tcatattgct gttgaaagac aggcgcgacg gcgtattcgg
    10261 ttcgcagatt gccaaacgcc tttctgggat gtttacgctg gttgccgtac tgcccggcgt
    10321 gtttctgttc ggcgtttccg cacagttcat caacggtacg attaattcgt ggttcggcaa
    10381 cgatacccac gaggcgcttg aacgcagcct caatttgagc aagtccgcat tgaatctggc
    10441 ggcagacaac gcccttggca acgccatccc cgtgcagata gacctcatcg gcgcggcttc
    10501 cctgcccggg gatatgggca gggtgctgga acattacgcc ggcagcggtt ttgcccagct
    10561 tgccctgtac aatgccgcaa gcggcaaaat cgaaaaaagc atcaacccgc acaagctcga
    10621 tcagccgttt ccaggtaagg cgcgttggga aaaaatccaa cgggcgggtt cggtcaggga
    10681 tttggaaagc ataggcggcg tattgtacgc gcagggctgg ctgtcggcgg gtacgcacaa
    10741 cgggcgcgat tacgccttgt ttttccgcca gccgattccc aaaggcgtgg cagaggatgc
    10801 cgtcttaatc gaaaaggcaa gggcgaaata tgctgagttg agttacagca aaaaaggttt
    10861 gcagaccttt ttcctggcaa ccctgctgat tgcctcgctg ctgtcgattt ttcttgcact
    10921 ggtcatggca ctgtatttcg cccgccgttt cgtcgaaccc gtcctatcgc ttgccgaggg
    10981 ggcaaaggcg gtggcgcaag gcgatttcag ccagacgcgc cccgtgttgc gcaacgacga
    11041 gttcggacgc ttgaccaagt tgttcaacca catgaccgag cagctttcca tcgccaaaga
    11101 agcagacgag cgcaacagga ggcgcgaaga agccgccaga cattatcttg aatgcgtgtt
    11161 ggaggggctg accacgggcg tggtggtgtt tgacgaacaa ggccgtctga aaaccttcaa
    11221 caaagcggcg gaacagattt tggggatgcc gcttaccccc ctgtggggca gcagccggca
    11281 cggttggcac ggcgtttcgg cgcagcagtc cctgcttgcc gaagtgtttg ccgccatcgg
    11341 cgcggcggca ggtacggaca aaccggtcca tgtgaaatat gccgcgccgg acgatgccaa
    11401 aatcctgctg ggcaaggcaa ccgtcctgcc cgaagacaac ggcaacggcg tggtaatggt
    11461 gattgacgac atcaccgttt tgatacacgc gcaaaaagaa gccgcgtggg gcgaagtggc
    11521 caaacggctg gcacacgaaa tccgcaatcc gctcacgccc atccagcttt ccgccgaacg
    11581 gctggcgtgg aaattgggcg ggaagctgga tgagcaggat gcgcaaatcc tgacgcgttc
    11641 gaccgacacc atcgtcaaac aggtggcggc attgaaggaa atggtcgaag cattccgcaa
    11701 ttatgcgcgt tcctcttcgc tcaaattgga aaatcaggat ttgaacgcct taatcggcga
    11761 tgtgttggca ttgtatgaag ccggtccgtg ccggtttgcg gcggagcttg ccggcgaacc
    11821 gctgatggtg gcggcggata cgaccgccat gcggcaggtg ctgcacaata ttttcaaaaa
    11881 tgccgccgaa gcggcggaag aagccgatgt acccgaagtc agggtaaaat cggaagcggg
    11941 gcgggacggt cggattgtcc tgacggtttg cgacaacggc aaagggttcg gcagggaaat
    12001 gctgcacaac gccttcgagc cgtatgtaac ggacaaaccg gcgggaacgg gattgggtct
    12061 gcctgtggtg aaaaaaatca ttgaagaaca cggcggccgc atcagcctga gcaatcagga
    12121 tgcgggcggc gcgtgtgtca gaatcatctt gccaaaaacg gtagaaactt atgcgtagca
    12181 gcgatatttt aattgtagac gacgaaatcg gcatccgcga cctgctgtcg gaaatcctgc
    12241 aggacgaagg ttattcggtc gcattggcgg aaaacgccga agaggcgcgc aagctgcgcc
    12301 atcaggcgcg ccccgcgatg gtgctgctgg atatttggat gcctgattgc gacggcatca
    12361 cccttttgaa ggagtgggcg aaaaacgggc agctcaatat gccggtggtg atgatgagcg
    12421 ggcatgccag catcgacaca gcagtggaag ccaccaaaat cggcgcgctc gactttttgg
    12481 aaaaaccgat ttccctgcaa aagctgctgt ccatggttga aaacgcgttg aagtacggtg
    12541 cggcgcaaac cgaaacgggg cctgtattcg acaagctggg caacagtgcc gcgattcagg
    12601 agatgaactg cgaagtcgaa gcggcggcaa agcgcacgtc ccctgtattt ttgacgggcg
    12661 aagcgggttc gccgtttgaa accgtggcgc gctatttcca taaaaacggt acgccgtggg
    12721 tcagtccggc aagggtcgaa tatctgatcg atatgccgat ggaactgttg cagaaggcgg
    12781 agggcggcgt tttgtatgtc ggcgacatcg cccagtacag ccgcaacatc caagccggta
    12841 ttgcctttat tgtcggaaag gcggaacacc gccgcgtcag ggtggtcgca tcgggcagca
    12901 gggcggcagg ttcagacggc attgcctgcg aggaaaagct ggcggaactg ctgtcggaat
    12961 cggtcgtccg tattccgccg ctgcgtatgc agcatgaaga cattcccttc ctgatacagg
    13021 ggattgcctg caatgtggcg gaaagccaaa agattgcgcc tgcctcattc agtgaagagg
    13081 cacttgccgc attgacccgt tacgactggc cgggaaattt cgaccaactg caaagcgtcg
    13141 ttgcaacgct gttgttggag gcggacggac aggaaatcgg cgcaggggcg gtttcttccc
    13201 ttttggggca gaatgtgcct gccgagggag cggaagatat ggtgggcggg tttaatttca
    13261 acctgcccct gcgcgaattg agggaggagg tggagcggcg ttatttcgag taccacatcg
    13321 cccaagaagg tcagaatatg agcaaagtgg cgcagaaagt cggtttggaa cgcacgcacc
    13381 tttaccgcaa actcaaacag ctcggcatcg gcgtttcgcg ccgggcgggg gaaaaaaccg
    13441 aagaataggc ccggacggcc ggttttccgg ctgcgggctt ttgttttcag acggcatttg
    13501 gtgcaaatgc cgtctgaaat cgtaagggga cggattttat gacagaggac gaacgtttcg
    13561 cgtggttgca actggctttt acgccctata tcggtgcgga aagtttcttg ctgctgctgc
    13621 accatttcgg cagtgcgcaa aatgccctgt ccgcaccggc ggaacaggtg gcggcactga
    13681 tacggcacaa acaggcgctt gaggcttggc gcaatgcgga aaaacgcgcc ttggcgcggc
    13741 aggcggcaga agcggcgttg gaatgggaaa tgcgggacgg atgccgtctg atgctgcttc
    13801 aggatgaaga tttccccgaa atgctggcgc aggggctgac cgcgccgcca gttttgtttt
    13861 tgcgcggcaa cgtgcggctg ctgcacaagc cttccgccgc catcgtcggc agccgctatg
    13921 ccacgccgca ggcgatgcgg attgccaaag atttcggcag ggcgttgggt gggaaaggca
    13981 ttcccgtcgt gtcgggtatg gcttcgggca tcgacaccgc cgcccatcag ggcgcgttgc
    14041 aggcagaagg cggcaccatc gccgtgtggg ggacgggcat agaccgcatt tatccgccgt
    14101 ccaataaaaa ccttgcctat gaaatcgccg aaaaagggct gattgtcagc gagttcccca
    14161 tcggtacgcg gccgtatgcc ggcaactttc cgcgccgcaa ccgcctgatt gccgcgctgt
    14221 cgcaggttac gctggtggtt gaagctgcgt tggaatccgg ttcgctgatt accgccggct
    14281 tggcggcaga aatggggcgc gaagtgatgg cggtacccgg ttcgatagac aatccgcaca
    14341 gcaaaggctg ccacaaactg attaaagacg gcgcgaaatt ggtggaatgc ctggacgaca
    14401 tcctgaacga atgcccgggg ctattgcaaa atacgggtgc ttcatcatat tctataaata
    14461 aggatacgcc cgacacggga cgccgcgccg ttcagacggc atacgccccg ccgcctgccg
    14521 caaaaatgcc gtctgaagcg gcggcaggcg gcacagccca cggcggcata ctggataaaa
    14581 tgggtttcga ccccgtccat cccgacgtgc ttgccgagca gcttgctatg cctgccgcag
    14641 atttgtatgc cgcactgttg gaattggaat tggacggcag cgttgccgca atgcccggcg
    14701 gcagatacca gcgtatccga acttgaacgc actttatatt aaggaacacg aatgaccgaa
    14761 gtcatcgcct acctcatcga acatttccaa gatttcgata cctgtccgcc gcccgaagac
    14821 ttgggtatgc tgcttgaaga agcgggtttc gataccatgg aaatcggcaa caccctgatg
    14881 atgatggaag tattgctcaa cagctccgaa ttttccgccg aacctgccga cagcggcgca
    14941 ttgcgcgtgt acagcaaaga agaaaccgac aacctgccgc aggaagtgat ggggctgatg
    15001 cagtatctga ttgaagaaaa agccgtcagc tgcgaacagc gggaaatcat catccacgcg
    15061 ctgatgcaca tccccggcga cgaaattacc gtagataccg ccaaagtgct gaccctgctg
    15121 cttttatggg caaacaagag cgagctgccc gtgttggtcg gcgacgagct gatgagcgcg
    15181 cttttactcg acaacaaacc cacgatgaac tgaagcggct tcagacggcc cgcccgaggc
    15241 cgtctgaaac gccggcatca aaaccaccat ccagagaacg acaaatggcg aaaaacctat
    15301 taatcgtcga atccccgtcc aaagccaaaa ccctgaaaaa atatttgggc ggcgattttg
    15361 aaatccttgc atcctacgga cacgtccgcg acctcgtccc caaaagcggc gcggtcgatc
    15421 ccgacaacgg ctttgcgatg aaataccaac tcatcagccg caacggcaaa cacgtcgatg
    15481 ccatcgtcgc cggtgccaaa gaagctgaaa acatctacct cgccaccgac ccggataggg
    15541 aaggcgaagc catttcctgg catcttttgg aaatcctcaa atccaaacgc ggcttgaaaa
    15601 acatcaagcc gcagcgtgtc gtgttccacg aaatcaccaa aaacgccgtg ctcgatgccg
    15661 ttgcccatcc gcgcgaaatc gaaatggact tggtcgatgc gcaacaagcc cgccgtgctt
    15721 tggactattt ggtcggcttt aacctttcgc cattgttatg gaaaaaaatc cgccgcggtt
    15781 tgagcgcggg tcgtgtgcaa agtcccgctt tgcgcctgat ttgcgaacgc gaaaacgaaa
    15841 tccgcgcgtt tgaagcgcag gaatattgga cggtgcatct cgacagccac aaaggccgca
    15901 gcaagttcac cgccaaactc gcccaataca acggcgcgaa actcgaacaa ttcgacctgc
    15961 cgaacgaagc cgcgcaagcc gacgtattga aagaactcga aggcaaagaa gccgtcgtta
    16021 ccgccattga aaagaaaaag cgcagccgca atcctgccgc gccgttcacc acttctacca
    16081 tgcagcagga tgccgtgcgc aaactcggct tcaccaccga ccgcaccatg cgtaccgccc
    16141 aacagcttta tgaaggtata gacgtagggc agggcgcgat cggtctgatt acctatatgc
    16201 gtaccgacag cgtgaatttg gcggatgaag cgttaaccga aatccgccat tacattgaaa
    16261 acaaaatcgg caaagaatat ctgccgagtg ccgccaaaca atacaaaacc aaatccaaaa
    16321 acgcccaaga agcccacgaa gccatccgtc cgacttccgt gtaccgcacg cccgaaagcg
    16381 tcaaaccctt cctgagcgca gaccagttca aactctatca aatgatttgg cagcgcaccg
    16441 tcgcctgtca gatgacgcct gccaaattcg accaaaccac cgtcgatatt accgtcggca
    16501 aaggcgtatt ccgcgtaacc ggacaagtgc aaaccttcgc aggctttttg agcgtgtatg
    16561 aagaaagcag cgatgacgaa gaaggcgaag acagcaaaaa actgcccgaa atgagcgaag
    16621 gcgataaatt gcccgtggac aaactctacg gcgaacaaca ctttaccact ccgccgccgc
    16681 gctacaacga agccacgctg gttaaagccc tcgaagaata cggcatcggc cgcccctcga
    16741 cctacgccag catcatctcc acgctcaaag accgcgaata cgttaccctt gagcaaaaac
    16801 gcttcatgcc caccgacaca ggcgacatcg tcaacaaatt cctgaccgaa cacttcgccc
    16861 aatacgtcga ttaccacttc accgccaaac tcgaagacca gcttgacgaa attgccgacg
    16921 gcaaacgcca atggattccc ttgatggaca aattctggaa accgttcatc aaacaagtgg
    16981 aagaaaaaga aggaatcgaa cgcgccaaat ttaccacgca ggaacttgat gaaacctgcc
    17041 cgaaatgcgg cgaacacaaa ctgcaaatca aattcggcaa aatgggtcgt tttgttgcgt
    17101 gtgccggtta tcccgagtgc agctacacgc gcaatgtcaa cgaaaccgcc gaagaggccg
    17161 ccgagcgcat cgccaaagcc gaagccgaac aggccgaact cgacggacgc gagtgcccga
    17221 aatgtggcgg tcgcctagtg tacaaataca gccgcaccgg cagcaaattc atcggctgcg
    17281 ccaactatcc gaaatgcaaa cacgtcgagc cgctggaaaa accgaaagat accggcgtcc
    17341 agtgtccgca atgcaaaaaa ggcaacctcg tcgagcgcaa atcccgctac ggcaaactgt
    17401 tttacagttg cagcacctat cccgactgca actacgccac ttggaacccg cccgttgccg
    17461 aagaatgccc gaactgccat tggccggtct tgaccatcaa aaccaccaaa cgctggggcg
    17521 tcgaaaaagt ctgcccgcaa aaagaatgcg gctggaaaga acagattgaa ccgcccgcgc
    17581 cgaaggagta agattaggtt ggtttgaaag agaaaaggtc gtctgaaaaa ttttcagacg
    17641 acctttgctt ttctgtgatt ggtttatttg aatccgcgtg ttgttttaaa gtccgataaa
    17701 atccggttca tttcaggcgc aaacaaggcg atgtaatcgt aagatagacc gcgactggca
    17761 ctgggatggg gaaagcagac gacttcgcaa tcttcaaacg attggaattt gacattgaaa
    17821 cgtgtaccgt caaattcttt ttgcaccgtc tccagcggtt tggtctgctt accgaccaac
    17881 tgctcgaagc gtggcagtac attttggttg ttcagaaaat ccgccaacct gctgcccatg
    17941 aagaggatga ctttcggacg cagtttttcg atgtggtaga gaaaattatc gatgtgctcg
    18001 ggttgtgtga acttgtcggg attgtcgata gtgttgccct gtgtagcagc ccagttggtt
    18061 tgaaccaggg atttttcaaa tgcaccgccc aatccatttt cgtctaaggg gtgtccccac
    18121 atttcaaacc aattttttat cgtattgtcg taacgccact tctttgcctg ctctccgaaa
    18181 tagagggatt tgtttgcaaa tgtatggtcg attttgtttt cagggagttt gtattcacct
    18241 gctacataag cagcctcatc ggctttactc caaccccatt catacccaca aatcattaaa
    18301 ccatgtttgt cgttgtagcc tttgaacaga ctgttgctca aattcaaatc cttcatcatg
    18361 aactcttcct tttaaaattt aagagcgatt gacttcaatg tttttagatg gggtggaaaa
    18421 atccttgtgt aggcaacata aattcaataa atttcttgat aattcgaaac ctactaatag
    18481 cgcacctata aaagcttttt cattacgttc agcatgacgg tcacgtcgtt catatttttt
    18541 acgcttgctg ttccctgtta ttacagctaa gccaagtgat atggcgagaa ttgcccaaac
    18601 aatagtactt aataacaatt ttccccatac tatcaataag gaaagaaaaa accttttagt
    18661 attagatcga taggttataa tccatgccca tgaaaatgca ataagagtta tacataagat
    18721 acaagctgac aggatttctt tatttttaat taaataactt agagcgatga ggatgacagg
    18781 tgtcaagaaa ataatagtta catcccgata gctataaaag aaaactgccc tattttgaat
    18841 gtggagatgt gcacagaatc caatatagct aaggataata gttaatataa taaaaaaaga
    18901 ccaccaaggg taaagagata ggaattccat gttttccttt ttttgtaaaa aggaaaaaat
    18961 tttatctaag agttaattgc ctaaccagcc agaagaagtt taaaatctat cccaataatt
    19021 caaccatcta tacagaaagt tcagcttatg gaaacccacg aaaaaatccg cctgatgcgc
    19081 gaattgaata aatggtccca ggaggatatg gcggaaaagc tggcgatgtc ggcaggcggg
    19141 tatgccaaaa tcgaacgggg cgaaacgcag ttaaatatcc cgcgtttgga gcagttggct
    19201 cagattttca aaatcgatat gtgggacttg ctcaaatcgg gcggtggtgg gatggtgttt
    19261 cagattaatg aaggtgatag tggtggcgat attgcgttgt atgcgtcggg tgatgtttcg
    19321 atgaaaatag aatttttaaa aatggagttg aaacactgca aagaaatgtt ggaacaaaaa
    19381 gacaaagaaa tcgagctgct ccgcaagctg accgaaaccg tttaaacaga tatgccgtct
    19441 gaaaaaagtt ttcagacggc atattctttg acaggtcttg tataataccg tttgaactta
    19501 caggtttttg attatggcgg caggcaaaca taccaaacac agcaaccggg tacgcattat
    19561 cggcgggcaa tgccggggca ggaaattgag tttcacatcc gccgacggac tgcgtccgac
    19621 acccgacagc gtgcgtgaaa agctgtttaa ctggctggga caggatttga cgggtaaaac
    19681 ggttttggat ctcttcggag gcagcggcgc actcggtatg gaggcggcat cgcgtcatgc
    19741 aaaacggatc gtgattgcag acaacaaccg tcaaacggta caaacccttg agaaaaacag
    19801 ccgcgaactg ggtttggggc aggtgcaaat cgtctgttca gacggcatcg catatttgaa
    19861 gaccgtatcc gaacagtttg atgttgtctt tctcgacccg ccgtttgcat ggcaggactg
    19921 gcaaatcctg tttgatgcct tgaagccgtg cctgaacccc cgagcattcg tctatctcga
    19981 ggcgggtacg ctgccggata ttcccgattg gctgacggaa tatagagaag ggaaatcggg
    20041 gcagagtaca ttcgaattaa gggttttcca agtggctgaa taatatgcgc tttgataatc
    20101 atttccgagt tgtaaacatt cgtttgcaac cgtccggttc aaaaaaacct tgtgctataa
    20161 tccgcgcccg cccggttttg ataatttagt ggaaaaggaa aagaaatgtc gctttttatt
    20221 accgacgagt gcatcaactg cgacgtatgc gaacccgaat gccccaatga tgccatttcc
    20281 caaggcgagg aaatttacga aatcaacccc aacctctgca cgcagtgcgt cggacactac
    20341 gatgagccgc agtgccagca ggtttgcccg gtggactgca tcctgattga cgaagaacat
    20401 cccgaaaccc atgacgagtt gatggcgaaa tacgaaaaga ttatccagtt taaataaatt
    20461 ctttttaaaa catcaaatta tgtctgtttt gaaataaaat caaaaaaaaa cttgacggaa
    20521 aagcaagccg ctaataaact aacgttctct tttggaggga ttcccgagcg gtcaaagggg
    20581 gcagactgta aatctgttgc gaaagcttcg aaggttcgaa tccttctccc tccaccaaaa
    20641 ttcttacttg gggcagtagc gagtaatgcg ggtgtagctc aatggtagag cagaagcctt
    20701 ccaagcttac ggtgagggtt cgattccctt cacccgctcc aaacaattag gcccatgtag
    20761 ctcaggggta gagcactccc ttggtaaggg agaggccggc agttcaaatc tgcccatggg
    20821 caccatctct cgattattca tttctttaag gcttagatat ataggatatt gccatggcta
    20881 aggaaaaatt cgaacgtagc aaaccgcacg taaacgttgg caccatcggt cacgttgacc
    20941 atggtaaaac caccctgact gccgctttga ctactatttt ggctaaaaaa ttcggtggtg
    21001 ctgcaaaagc ttacgaccaa atcgacaacg cacccgaaga aaaagcacgc ggtattacca
    21061 ttaacacctc gcacgtggaa tacgaaaccg aaacccgcca ctacgcacac gtagactgcc
    21121 cggggcacgc cgactacgtt aaaaacatga ttaccggcgc cgcacaaatg gacggtgcaa
    21181 tcctggtatg ttccgcagcc gacggcccta tgccgcaaac ccgcgaacac atcctgctgg
    21241 cccgtcaagt aggcgtacct tacatcatcg tgttcatgaa caaatgcgac atggtcgacg
    21301 atgccgagct gttggaactg gttgaaatgg aaatccgcga cctgctgtcc agctacgact
    21361 tccccggcga cgactgcccg attgtacaag gttctgcact gaaagccttg gaaggcgatg
    21421 ccgcttatga agagaaaatc ttcgaactgg ctgccgcatt ggacagctac atcccgactc
    21481 ccgagcgtgc cgtggacaaa ccgttcctgc tgcctatcga agacgtgttc tccatttccg
    21541 gccgaggtac agtagtaacc ggccgtgtag agcgcggtat catccacgtt ggtgacgaga
    21601 tcgaaatcgt cggtctgaaa gaaacccaaa aaaccacctg taccggtgtt gaaatgttcc
    21661 gcaaactgct ggacgaaggt caggcgggcg acaacgtagg cgtattgctg cgcggtacca
    21721 aacgtgaaga cgtggaacgc ggtcaggtat tggccaaacc gggtactatc actcctcaca
    21781 ccaaattcaa agcagaagtg tacgtactga gcaaagaaga gggcggccgc catactccgt
    21841 tcttcgccaa ctaccgtccg caattctact tccgtaccac cgacgtaacc ggcgcggtta
    21901 ctttggaaga aggtgtggaa atggtaatgc cgggtgagaa cgtaaccatt accgtagaac
    21961 tgattgcgcc tatcgctatg gaagaaggcc tgcgctttgc gattcgcgaa ggcggccgta
    22021 ccgtgggtgc cggcgtggtt tcttctgtta tcgcttaagt ttagaggcca atagctcaat
    22081 tggtagagta tcggtctcca aaaccgaggg ttgggggttc gagaccctct tggcctgcca
    22141 aataaaaaat taaccggcct tgtgtcggtt aatttttttg tatttgttat ttagtaaact
    22201 ctcttgccat ttacatggat tgagaataga cagatgctat gatggataaa taatatgaca
    22261 gaacatacgc ctgaaaaaaa gaacgttaaa gtggatcaac tggttgttca agataaagaa
    22321 tctgcatcta attccggtaa ggaagggttt tttgcatatt tctcaaattc ttggtccgaa
    22381 ttcaaaaagg tggtttggcc taagcgtgaa gatgctgtca gaatgactgt atttgttata
    22441 gtgtttgttg ctgtgctttc tatatttatc tatgcggcag atacagcaat ttcgtggtta
    22501 ttttttgatg tattgctgag aagggaaggt tgagatgtcg aaaaaatggt atgttgtaca
    22561 ggcgtattcg gggtttgaga agaatgtcca acgaatattg gaagagcgca ttgcccgtga
    22621 ggagatggga gattatttcg gacaaattct ggtgcctgta gagaaagttg ttgatatccg
    22681 caatggtcgt aagactatta gtgaaagaaa gtcatatcct ggttatgtgc tagttgagat
    22741 ggaaatgaca gatgactctt ggcatcttgt aaaaagcacc ccccgtgttt ccggttttat
    22801 tggagggagg gctaatagac ctacgccgat tagtcagaga gaggctgaaa ttattttaca
    22861 gcaggttcag accggcatag agaagccgaa accaaaagtt gaatttgagg tcggtcaaca
    22921 ggttcgtgta aatgaagggc cgtttgcgga ttttaacggg gtggttgagg aggtcaatta
    22981 tgaacggaat aagttacgcg tgtctgttca gatatttggt agagaaacac ccgttgagct
    23041 ggagttcagc caggttgaaa agattaactg atttttatac ttgaaaaaaa agcaataaga
    23101 ggatagaatc aaaaattaac ttggggagcg gaaatggttc cgcgtcttac ccgtttttag
    23161 gagttcgtta agtggcaaag aaaattatcg gctatattaa actgcaaatt cctgcaggta
    23221 aagccaatcc atctcctccg gttggtcctg ctttgggtca gcgcggtttg aatattatgg
    23281 aattttgtaa ggcatttaat gctgcaaccc aaggtatgga gcctggctta ccgattccgg
    23341 ttgtgattac tgcatttgca gataaatcat tcacatttgt gatgaaaacc ccgccagctt
    23401 ctatcttgtt gaaaaaggct gccggtttgc aaaaaggtag ttctaatcct ctgaccaaca
    23461 aagtgggtaa attgacccgt gcccagttgg aagaaattgc taaaactaaa gatcctgatt
    23521 tgactgctgc tgacttggat gcggctgtcc gtactatagc aggttctgct cgctcaatgg
    23581 gcttggatgt ggagggtgtt gtataatggc taaagtatct aaacgcttga aagctcttcg
    23641 ctcttctgtg gaagccaata aattatatgc aattgatgaa gcaattgctt tggtaaaaaa
    23701 agcagcgact gctaaatttg acgagtctgt tgacgtatct ttcaacttgg gcgttgatcc
    23761 gcgtaaatct gaccaagtta tccgtggatc ggtcgttctg cctaaaggca ccggtaagat
    23821 aacccgtgtg gctgtattta ctcaaggtgc aaatgcagaa gctgctaaag aagctggtgc
    23881 agatatcgtc ggtttcgaag atttggctgc tgaaatcaaa gcgggcaatc tgaactttga
    23941 cgttgttatt gcttctcccg atgcaatgcg tattgttggt caattgggta ccatcttggg
    24001 tcctcgtggt ctgatgccaa accctaaagt aggtacggtt acccctaatg ttgccgaagc
    24061 agtaaaaaat gcaaaagcag gtcaagtgca gtaccgtacc gacaaggcag gtattgttca
    24121 tgcaacgatt ggtcgtgctt cattcgctga agctgatttg aaagagaact ttgatgcgtt
    24181 gctggatgct atcgttaaag ccaagcctgc tgccgctaaa ggtcagtatc tgaaaaaagt
    24241 tgctgtgtct agcaccatgg gtttgggtat tcgcgttgat acatcaagcg taaataacta
    24301 atcttaagga attttcaagc agtttggttt tctgggctgc ttgaatttgg gctacttaaa
    24361 attaagtaga tgtccaagac cgtagggatc gtaagattta atcgtaactg ccctacgcag
    24421 acggtagtcc tgaaacacat tgcaagattg cttgtaagat gtctttttag gttaccgcgc
    24481 tggtgggata tcgttttggt atcctgttta taaacagtgg gaggtagacc ttgagtctca
    24541 atattgaaac caagaaagtg gcggtcgagg aaattagcgc ggcaattgct aatgctcaaa
    24601 ccctcgtagt cgctgaatat cgcggtatca gtgtttccag tatgactgag cttcgtgcga
    24661 atgcacgtaa agaaggcgtt tatttgcgtg ttctgaaaaa tactttggct cgtcgtgcag
    24721 tgcaaggtac ttcatttgca gaattggccg atcaaatggt tggtccgttg gtttacgctg
    24781 cttctgaaga tgctgttgct gctgctaaag tgttgcacca attcgcgaaa aaagatgaca
    24841 aaattgtcgt taaagccggt tcttacaatg gcgaagtaat gaatgctgct caggttgctg
    24901 agttggcttc tattccgagc cgcgaagagc tgttgtccaa actgttgttc gttatgcaag
    24961 ctcctgtatc gggctttgcg cgcggtttgg ctgctttggc agagaaaaaa gccggcgaag
    25021 aagccgctta atcgattttg tttctgttaa tcaattattt tttaatacaa tatttggagt
    25081 aaaatagcat ggctattact aaagaagaca ttttggaagc agttggttct ttgaccgtaa
    25141 tggaattgaa cgacttggtt aaagcttttg aagaaaaatt tggtgtttct gctgctgctg
    25201 ttgcagttgc aggtcctgct ggtgccggtg ctgccgatgc tgaagaaaaa accgaatttg
    25261 atgtcgtttt ggcttctgcc ggcgatcaaa aagtcggcgt gattaaagtt gtccgtgcaa
    25321 ttaccggttt gggtctgaaa gaagctaaag acatcgttga cggcgcacct aaaaccatta
    25381 aagagggtgt ttctaaagct gaagccgaag acatccaaaa acaactggaa gaagcaggcg
    25441 ctaaagtcga aatcaaataa tttgatgctt cttatgaagg ctggcagttt tctgccagcc
    25501 ttattttgct tcttaaaata aacatcaagt attgtttaca tttatttgca tagtttttat
    25561 caagtcattg caaataaatg taaatatcag attgatgcgt accgttgttt cagacggcct
    25621 attattgaaa attacttttc ggagtgtgta tgaactattc gtttaccgag aaaaaacgta
    25681 tccgtaagag ttttgcaaag cgggaaaatg ttttggaagt tcctttcctg ctagcaactc
    25741 agattgattc ttatgcgaaa tttttgcaac tggaaaatgc ttttgacaaa cgtaccgatg
    25801 acggtctgca ggcggcattt aattctattt tcccgattgt gagccataac ggttatgcgc
    25861 gattggagtt tgtgcattac acattgggcg agcctttgtt cgatattccc gaatgtcagt
    25921 tgcgcggaat cacttatgca gcccccttgc gcgcgcgtat ccgtttggtg attttggata
    25981 aggaagcatc taaaccgacg gtaaaagaag ttcgtgaaaa cgaagtgtat atgggcgaaa
    26041 ttccgttgat gaccccgagc ggttcttttg tgattaacgg cacagagcgt gtgattgtct
    26101 cccagttgca ccgttcgccc ggcgtattct tcgagcatga caaaggtaag acgcactctt
    26161 ccggcaaatt gttattctcc gcccgcatca ttccctaccg tggttcatgg ttggattttg
    26221 aatttgatcc gaaagatttg ctgtatttcc gtatcgaccg ccgccgtaaa atgccggtaa
    26281 cgattttgtt gaaggctttg ggctacaaca atgagcaaat cttggatatt ttctacgaca
    26341 aagaaacgtt ctatttgtct tcaaacggtg ttcaaaccga tttggtcgca ggccgtctga
    26401 aaggcgaaac tgccaaggtc gatatcttgg ataaagaagg caatgtattg gttgccaaag
    26461 gtaagcgcat tactgcgaaa aatatccgtg atattaccaa tgcaggcctg acccgtttgg
    26521 atgtagaacc ggaaagcctg ctgggtaaag cattggctgc cgatctgatt gattcggaaa
    26581 ccggcgaggt attggcttct gccaatgatg aaattacaga agagttgttg gccaaatttg
    26641 atatcaacgg cgtaaaagaa attacgaccc tttacatcaa tgagttggat cagggtgctt
    26701 atatctccaa caccttgcgt acggatgaga ctgccggccg gcaggcggcg cgtgttgcga
    26761 tttaccgtat gatgcgtccg ggcgaaccgc ccaccgaaga ggcggtcgag caattgttta
    26821 accgcttgtt cttcagtgaa gacagctacg atttgtcccg cgtaggccgt atgaaattta
    26881 atacgcgcac atacgaacaa aaactgtccg aagcccaaca aaactcttgg tacggccgcc
    26941 tgctgaacga aacgtttgcc ggtgctgccg aaaaaggcgg ttatgtcctg agcgtcgaag
    27001 atattgtcgc ctcgattgcg actttggtcg agttgcgtaa cggccatggt gaagttgacg
    27061 atatcgatca cttgggcaac cgccgagtac gttcggtagg cgagctgact gaaaaccaat
    27121 tccgcagcgg tttggcccgt gtggaacgtg ctgtaaaaga acgtttgaat caggcggaat
    27181 cagaaaactt gatgccgcac gatttgatta atgcaaaacc tgtttctgcc gctattaaag
    27241 aattcttcgg ctccagccaa ttgagtcagt ttatggatca gaccaacccc ttgtctgaag
    27301 taacccataa acgccgcgta tccgcattgg gtccgggcgg tttgacccgc gaacgtgcag
    27361 gatttgaggt gcgggacgtg catccgaccc actacggccg cgtatgtccg attgaaacgc
    27421 ctgaaggtcc gaacatcggt ttgatcaact cattgtccgt ttatgcgcgc accaatgatt
    27481 acggtttctt ggaaacgcct taccgccgcg ttatcgacgg caaagtaacc gaggaaatcg
    27541 attacttgtc tgccatcgaa gaaggccgct atgtgattgc acaggcgaat gccgatttgg
    27601 attcagatgg caatctgatt ggcgatttgg ttacctgtcg tgaaaaaggc gaaaccatta
    27661 tggcaacgcc cgaccgcgtc caatatatgg acgtggcaac tggtcaagtg gtatccgttg
    27721 cagcatccct gattccgttc ttggaacatg atgacgcgaa ccgcgcattg atgggtgcca
    27781 acatgcaacg tcaggcagtg ccttgcttgc gtcctgaaaa accgatggtc ggtaccggta
    27841 tcgagcgttc cgttgccgtt gactctgcta ctgcaatcgt tgcccgccga ggcggcgtgg
    27901 tcgagtatgt cgatgccaac cgtgttgtga ttcgtgtcca tgacgacgaa gcgactgccg
    27961 gcgaagtggg tgtcgatatt tacaacttgg ttaaatttac ccgttccaat caatctacta
    28021 acatcaacca acgtccggct gtcaaagcag gcgatgtttt gcaacgcggc gatttggtgg
    28081 ccgatggcgc gtccaccgat ttgggcgaat tggctttggg tcaaaatatg accatcgcct
    28141 tcatgccgtg gaacggttac aactacgaag actcgattct gatttccgaa aaagtggctg
    28201 cggacgaccg ctatacttcg attcacattg aggaattgaa tgtcgttgcc cgcgatacca
    28261 agctgggtgc ggaagacatt acccgcgata ttccgaactt gtccgagcgt atgcaaaacc
    28321 gtttggacga atccggtatc gtttacatcg gtgcggaagt agaagccggc gatgtgttgg
    28381 taggcaaggt aacgcctaaa ggcgaaaccc agctgacgcc ggaagaaaaa ctgctgcgcg
    28441 ccatcttcgg tgaaaaagca tctgacgtaa aagatacttc attgcgtatg cctaccggca
    28501 tgagcggtac cgttatcgac gttcaagtct tcactcgtga aggtattcaa cgcgacaaac
    28561 gtgctcaatc cattatcgat tccgagttga aacgctaccg tttggatttg aacgaccaat
    28621 tgcgtatttt cgacaacgac gcattcgacc gtatcgagcg tatgattgtc ggtcagaaag
    28681 ccaacggcgg cccgatgaag ctggccaaag gcagtgaaat cacgaccgaa tatctggcgg
    28741 gtctgccgag caggcacgat tggttcgata tccgtctgac cgatgaagat ttggccaagc
    28801 agttggaact gattaaagtg agcctgcaac aaaaacgcga agaagcggac gagttatacg
    28861 aaatcaagaa gaaaaaactg acccaaggcg acgaattgca acccggcgta caaaaaatgg
    28921 tgaaagtttt tatcgccatc aaacgccgtc tgcaagccgg cgacaaaatg gcgggccgcc
    28981 acggtaacaa aggcgtggta tcgcgcattc tgccagtgga agacatgcct tacatggcgg
    29041 acggccgtcc ggtagacatc gtactgaacc cattgggcgt accttcccgt atgaacatcg
    29101 gtcagatttt ggaagttcac ttgggttggg cagcaaaagg tatcggcgag cgtatcgacc
    29161 gtatgctgaa agagcaacgc aaagcaggcg agttgcgcga gttcttgaac aaactctaca
    29221 acggcagcgg caagaaagaa gatttggatg ccctgactga tgaagaaatc atcgaactgg
    29281 cctccaacct gcgtaaaggt gcatctttcg cctctcctgt attcgacggt gcgaaagagt
    29341 ctgaaatccg cgaaatgttg aacttggctt atccgagcga cgatcctgag gttgaaaaac
    29401 tgggcttcaa cgacagtaaa acccaaatca cgctgtatga cggccgttca ggcgaagcat
    29461 ttgaccgcaa ggttacagtc ggtgtgatgc actatctgaa actgcaccac ttggttgacg
    29521 aaaaaatgca cgcgcgttct accggtccgt acagtctggt tacccagcag cctttgggcg
    29581 gtaaagccca gttcggcggc caacgtttcg gcgagatgga ggtttgggca ttggaagcat
    29641 acggcgcggc atacacgctg caagagatgc tgactgtgaa gtctgacgac gtgaacggcc
    29701 gtaccaaaat gtacgaaaac atcgtcaaag gcgaacacaa aatcgatgcc ggtatgcccg
    29761 agtccttcaa cgtattggtc aaagagattc gctcactggg cttggatatc gatttggaac
    29821 gttactaaac aaaagttttc agacggcctt tcagggtcgt ctgaaaaagt ggtttcagaa
    29881 taagaatgaa gcaatcggca tttaggccgt ctgaaatcaa aagtaccgtt tccccatatc
    29941 gaaaatccgc catgcggtaa aaatacttcc ttcaaggagc aaaaatgaat ttgttgaact
    30001 tatttaatcc gttgcaaact gccggcatgg aagaagagtt tgatgccatt aaaatcggta
    30061 ttgcctctcc cgaaaccatc cgctcatggt cttatggcga agtcaaaaaa cctgaaacca
    30121 tcaactaccg tacgttcaaa cctgagcgcg acggtttgtt ctgtgccaaa atctttggcc
    30181 cggtcaaaga ctacgaatgc ttgtgcggaa aatacaaacg cttgaaattt aaaggcgtaa
    30241 cgtgtgaaaa atgcggcgtg gaagtaaccc tgtccaaagt gcgccgcgaa cgcatgggtc
    30301 atatcgaatt ggctgcgccc gtcgcacata tttggttctt aaaatccctg ccttcccgct
    30361 tgggtatggt gttagacatg actttgcgcg acatcgagcg cgtattgtac tttgaagcat
    30421 ttgtggtaac cgatcccggt atgactccgc tgcaacgccg ccaattgctg actgaagacg
    30481 attactacaa caagctggac gaatacggcg acgacttcga tgccaaaatg ggtgcagaag
    30541 gtatccgcga attgttgcgt accctgaatg tagcgggcga aatcgaaatc ctgcgccaag
    30601 agttggaatc gactggttcc gacaccaaaa tcaaaaaaat cgccaaacgc ttgaaagtat
    30661 tggaagcctt ccatcgttcc ggtatgaaac tggaatggat gattatggat gtgctgccgg
    30721 tattgccgcc tgatttgcgt ccgttggttc cattggatgg tggtcgtttt gccacttccg
    30781 atttgaacga tttgtaccgc cgcgttatta accgtaacaa ccgtctgaaa cgtctgttgg
    30841 aactgcacgc gcctgacatc atcgtccgca acgaaaaacg tatgttgcaa gaagcggtcg
    30901 actcgctgtt ggataacggc cgtcgcggta aagccatgac cggcgccaac aaacgtccgc
    30961 tgaaatcatt ggctgacatg attaaaggta aaggcggccg cttccgtcaa aacctgctgg
    31021 gtaaacgtgt ggactactct ggtcgttccg tgattaccgt aggtccatac ctgcgcctgc
    31081 accaatgtgg tctgccgaaa aaaatggctt tggaactgtt caaaccgttc attttccaca
    31141 aattggaaaa acaaggtctg gcttccaccg ttaaagcagc gaaaaaattg gtagaacaag
    31201 aagtgccgga agtatgggac atcttggaag aagtcattcg cgaacatccg attatgctga
    31261 accgtgcgcc aaccctgcac cgtttgggta ttcaagcgtt cgaacctatc ctgattgaag
    31321 gtaaagcgat tcagttgcac ccattggtgt gtgccgcatt taacgccgac tttgacggtg
    31381 accaaatggc tgtacacgtc ccattgagct tggaagcaca aatggaggca cgcacgctga
    31441 tgctggcttc aaacaacgta ttgtctccgg ccaacggcga accgattatc gtaccttccc
    31501 aagacatcgt attgggcctg tactacatga cccgcgaccg tatcaatgcc aaaggcgagg
    31561 gcagcctgtt tgccgatgtg aaagaagtgc atcgcgcata ccataccaaa caggttgaac
    31621 tgggtacgaa aattaccgta cgtctgcgcg aatgggtgaa aaacgaagca ggtgagtttg
    31681 agcctgtcgt taaccgttac gaaacaaccg tcggccgtgc attgttgagc gaaatcctgc
    31741 cgaaaggcct gccgtttgaa tatgtcaaca aagcgttgaa gaaaaaagaa atttctaaac
    31801 tgattaacgc atcgttccgc ctgtgcggct tgcgcgatac ggttatcttt gctgaccacc
    31861 tgatgtacac cggtttcgga tttgcggcaa aaggcggtat ttccattgcc gttgacgata
    31921 tggaaattcc aaaagaaaaa gcggccttgc tggctgaagc caatgccgag gttaaagaaa
    31981 tcgaagacca ataccgtcaa ggtttggtta ccaacggcga acgctacaac aaggtggtcg
    32041 atatttgggg tcgtgccggc gataagattg ctaaagcgat gatggacaac ttgtccaaac
    32101 aaaaagttat cgaccgtgac ggcaacgaag tcgatcaaga gtcattcaac tccatttata
    32161 tgatggcgga ctccggtgcc cgtggttctg cagctcagat taaacagttg tccggtatgc
    32221 gtggcttgat ggctaaacct gacggctcga ttattgaaac gccgattacc tcaaacttcc
    32281 gtgaaggtct gaccgtattg caatacttta ttgcgaccca cggtgcgcgt aagggtttgg
    32341 cggataccgc attgaaaacc gcgaactccg gttacctgac tcgtcgtctg gtagacgtaa
    32401 ctcaagattt ggtcgttgtt gaagacgatt gcggtacttc agacggcttt gtcatgaagg
    32461 cagtggtaca aggcggtgat gtgattgaag cattgcgcga tcgtattttg ggtcgtgtta
    32521 ccgcgtctga cgttgtcgat ccgtcaagtg gcgaaacctt ggttgaagcc ggtacgttgc
    32581 tgactgaaaa actggtggat atgatcgacc aatccggtgt cgatgaagtc aaagtccgta
    32641 cgccgattac ttgtaaaacc cgtcacggcc tgtgtgcaca ctgttacggt cgtgacttgg
    32701 cacgcggcaa actggttaac gccggtgagg cagtcggtgt gattgctgca caatccattg
    32761 gcgaaccggg tacccagttg accatgcgta cgttccacat cggtggtgcg gcatcccgtg
    32821 cggcagcagc cagccaagtg gaagccaaat ccaacggtac ggcacgattc agcagccaga
    32881 tgcgctacgt tgccaacaac aaaggcgagt tggttgtcat cggccgttct tgtgaagtcg
    32941 tgattcacga cgatatcggc cgtgaacgcg aacgccacaa agtaccttac ggtgccatcc
    33001 tgctggtaca agacggtatg gccattaaag ccggtcaaac cttggcaacc tgggatccgc
    33061 atacccgtcc gatgattacc gaacacgcag gtatggtgaa attcgaaaac gtggaagagg
    33121 gcgttaccgt tgccaaacaa accgatgatg taaccggttt gtccactttg gtggtgattg
    33181 acggtaaacg tcgttcctct agtgcttcca aactgctgcg tccgactgtg aaactcttgg
    33241 acgaaaacgg cgtggaaatc tgtattcccg gtacttctac tccggtatcc atggcattcc
    33301 ccgttggtgc ggtgattacc gtacgcgaag gtcaggaaat cggtaaaggc gacgtattgg
    33361 cgcgtattcc gcaagcctct tccaaaaccc gcgacattac cggcggcctg ccgcgcgttg
    33421 ccgagctgtt tgaagcacgc gtgccgaaag atgccggtat gttggcggaa attaccggta
    33481 ccgtttcctt cggcaaagag accaaaggga agcaacgtct gattatcact gacgtggacg
    33541 gtgtagcata cgaaaccctg atttctaaag agaaacagat tctggtacac gacggtcaag
    33601 tggtaaaccg cggcgaaacc attgtggacg gcgcggtcga tccgcacgat attctgcgtc
    33661 tgcaaggtat cgaagcactg gcacgctaca ttgtccaaga ggtgcaagag gtttaccgtc
    33721 tgcaaggtgt gaagatttct gataaacaca tcgaagtcat catccgtcaa atgttgcgcc
    33781 gtgtgaacat tgcggatgcc ggcgaaaccg ggttcattac cggagagcag gtcgaacgcg
    33841 gcgatgtgat ggcggccaat gaaaaagctt tggaagaagg caaagagccg gcacgttacg
    33901 aaaacgtatt gctgggtatt accaaagcct ccctgtccac cgacagtttc atttctgccg
    33961 catcgttcca agaaacgacc cgtgttctga ccgaagccgc gattatgggc aaacaagacg
    34021 aactgcgcgg tttaaaagag aacgtcatcg tcggccgcct gattcctgcc ggtaccggtt
    34081 tgacttatca ccgcagccgt catcagcaat ggcaaggggt agagcaggag actgctgaaa
    34141 cccaagtaac ggatgaataa tcttcggcgc gtttattcaa tacaaaaccg caagctttaa
    34201 gcttgcggtt tttctttgtc cgattaaggc aaaaacaagc gttttcgtca ttttgaggcg
    34261 tgtggattat tccttaggta ttttcgggcc ggagaccaac gaggtggcgg gtgtcgtcgg
    34321 tacgtccgga gaccaaaata actttgccag ggatgttggt ttcggcggtc aaaaaaagta
    34381 gcgtcttaat gttttccatt taaacaaatg tcgtctgaaa cttcagacgg catttccttt
    34441 aaaaaataaa tatgaaaccc agaaatctct tttttgcagg ctgcctgctg acttcggcga
    34501 cgtttgccga ggatatcggc gtacctgtcg aactgattaa cgtcggtaat cggattgcga
    34561 tgccgtctga aggggaaagc ctcgccctcc tgccgtttgc cgaggatgta ccgccggttc
    34621 gcgatgcaat gccgtctgaa gttcctaaaa gcgcggcagg cggcgatgtt cggggtgacc
    34681 ggatgggaat gccgattaac atcggatgag cgcggcttta tggcataaaa aactgtcgtg
    34741 gaaaggattt acaccccaaa taaatttccg ttacaacaag atcaacagca atatgcccgc
    34801 cttttattcg cgcagcggca aggaacggtt tgtcagtata gaaaaaacgt attgacagta
    34861 ttttcttcag tcgtccgact gattgtgagg gatgtcggta aatatttatc ggcaaacaag
    34921 aaaatcatct ttcttcttgt cgttatgctt gactgtatac ttgcaataaa aatataattc
    34981 cactcttgcc gacatggtgt cggcaagtat ttaactcaac aggacgagaa aatatgccaa
    35041 ctatcaacca attggtacgc aaaggccgtc aaaagcccgt gtacgtaaac aaagtgcccg
    35101 cactggaagc ctgcccgcaa aaacgcggcg tgtgcacccg tgtatacacg actaccccta
    35161 aaaaacctaa ctctgcattg cgtaaagtat gtaaagtccg cctgaccaac ggttttgaag
    35221 tcatttcata catcggcggc gaaggccaca acctgcaaga gcacagtgtc gtattgattc
    35281 gcggcggtcg tgtaaaagac ttgccaggtg tgcgttacca cactgtacgc ggttctttgg
    35341 atactgcagg cgttaaagac cgtaaacaag cccgttccaa atacggtgct aagcgtccta
    35401 aataattact gggacttaaa taggcacgtc ggccgcctaa gctgaacaac ggccgagtaa
    35461 gtgaatactc aattgggtat tcatgggaat agacccgact gaatagatta aaggaaatta
    35521 aaatgccaag acgtagagaa gtccccaagc gcgacgtact gccagatcct aaattcggca
    35581 gcgtcgagtt gaccaaattc atgaacgtat tgatgattga cggtaaaaaa tccgttgccg
    35641 agcgtatcgt ttacggtgcg ttggaacaga ttgagaaaaa aaccggcaaa gtagcaatcg
    35701 aagtatttaa cgaagccatt gcaaacgcca aacctatcgt ggaagtgaaa agccgccgtg
    35761 taggtggtgc aaactaccaa gttcctgttg aagttcgtcc ttcacgccgt ttggctttgg
    35821 caatgcgctg ggttcgcgat gcggcccgca aacgtggtga gaaatccatg gacctgcgtt
    35881 tggcaggcga attgattgat gcgtccgaag gccgtggcgg tgcgttgaaa aaacgtgaag
    35941 aagtacaccg tatggctgaa gccaacaaag cattctctca cttccgtttc taattttgaa
    36001 aggctaataa aatggctcgt aagaccccga tcagcctgta ccgtaacatc ggtatttccg
    36061 cccatatcga tgcgggtaaa accacgacga cagaacgtat tttgttctat accggtttga
    36121 cccacaagct gggcgaagtg catgacggtg cggctactac cgactacatg gaacaagagc
    36181 aagagcgcgg tattaccatt acctccgctg ccgttacttc ctactggtcc ggtatggcga
    36241 aacaattccc cgagcaccgc ttcaacatca tcgacacccc gggacacgtt gactttaccg
    36301 tagaggtaga gcgttctatg cgtgtattgg acggcgcggt aatggtttac tgcgcggtgg
    36361 gcggtgttca accccaatct gaaaccgtat ggcggcaagc caacaaatac caagtgccgc
    36421 gcttggcgtt tgtcaataaa atggaccgtc agggtgccaa cttcttccgt gttgtcgagc
    36481 aaatgaaaac ccgtttgcgc gccaatcctg tacctatcgt cattccggtt ggtgcggaag
    36541 acaacttcag cggtgtggtt gatttgttga aaatgaaatc catcatttgg aatgaagtcg
    36601 ataaaggtac aacctttacc tatggcgata ttcctgccga attggtcgaa actgccgaag
    36661 aatggcgtca aaatatgatt gaagccgcag ccgaagccag cgaagaactg atggacaaat
    36721 acttaggcgg cgacgagctg accgaagaag aaatcgtagg cgcgttgcgt caacgtactt
    36781 tggcaggcga aattcagcct atgctgtgtg gttctgcatt taaaaacaaa ggtgttcaac
    36841 gtatgttgga cgcagttgta gaattgctgc cagctcctac cgatattcct ccggttcaag
    36901 gtgtcaaccc gaataccgag gaagccgaca gccgtcaagc cagcgatgaa gagaaattct
    36961 ctgcattggc gttcaaaatg ttgaacgaca aatacgtcgg tcagctgacc tttatccgcg
    37021 tttactcagg cgtagtaaaa tccggcgata ccgtattgaa ctccgtaaaa ggcactcgcg
    37081 aacgtatcgg tcgtttggta caaatgactg ccgcagaccg tactgaaatc gaagaagtac
    37141 gcgccggcga catcgcagcc gctattggtc tgaaagacgt tactaccggt gaaaccttgt
    37201 gtgcggaaag cgcgccgatt atcttggaac gtatggaatt ccccgagccg gtaatccata
    37261 ttgccgttga gccgaaaacc aaagccgacc aagagaaaat gggtatcgcc ctgaaccgct
    37321 tggctaaaga agacccttct ttccgtgtcc gtacagacga agaatccggt caaaccatta
    37381 tttccggtat gggtgagctg cacttggaaa ttattgttga ccgtatgaaa cgcgaattcg
    37441 gtgtggaagc aaatatcggt gcgcctcaag tggcttaccg tgaaactatc cgcaaagccg
    37501 ttaaagccga atacaaacat gcaaaacaat ccggtggtaa aggtcaatac ggtcacgttg
    37561 tgattgaaat ggaacctatg gaaccgggtg gtgaaggtta cgagtttatc gatgaaatta
    37621 aaggtggtgt gattcctcgc gaatttattc cgtctgtcga taaaggtatc cgcgatacgt
    37681 tgcctaacgg tatcgttgcc ggctatcctg tagttgacgt acgtatccgt ctggtattcg
    37741 gttcttacca tgatgtcgac tcttcccaat tggcatttga attggctgct tctcaagcgt
    37801 ttaaagaagg tatgcgtcaa gcatctcctg ccctgcttga gccaatcatg gcagttgaag
    37861 tggaaacccc ggaagaatac atgggcgacg taatgggcga cttgaaccgc cgtcgcggtg
    37921 ttgtattggg tatggatgat gacggtatcg gcggtaaaaa agtccgtgcc gaagtacctt
    37981 tggcagaaat gttcggttac tcgaccgacc tgcgttctgc aacccaaggc cgcgctactt
    38041 actctatgga gttcaagaaa tattctgaag ctcctgccca catagctgct gctgtaactg
    38101 aagcccgtaa aggctaatca gaaaaggccg tctgaaactg aaaataaatt ttcagacggc
    38161 cattgttctt taatcgatct ttatatgtaa aggaattagc tcatggctaa ggaaaaattt
    38221 gaacgtagca aaccgcacgt aaacgttggc accatcggtc acgttgacca tggtaaaacc
    38281 actctgactg ctgctttgac tactattttg tctaaaaaat tcggtggcgc tgcaaaagct
    38341 tatgaccaaa tcgacaacgc tcctgaagaa aaagctcgtg gtattaccat taatacctca
    38401 cacgtagaat acgaaactga aacccgtcac tacgcacacg tagactgccc ggggcacgcc
    38461 gactacgtta aaaacatgat taccggcgcc gcacaaatgg acggtgcaat cctggtatgt
    38521 tccgcagccg acggccctat gccgcaaacc cgcgaacaca tcctgctggc ccgccaagta
    38581 ggcgtacctt acatcatcgt gttcatgaac aaatgcgaca tggtcgacga tgccgagctg
    38641 ttggaactgg ttgaaatgga aatccgcgac ctgctgtcca gctacgactt ccccggcgat
    38701 gactgcccga ttgtacaagg ttccgcactg aaagccttgg aaggcgatgc cgcttacgaa
    38761 gaaaaaatct tcgaactggc tgccgcattg gacagctaca tcccgactcc cgagcgagcc
    38821 gtggacaaac cgttcctgct gcctatcgaa gacgtgttct ccatttccgg ccgcggtaca
    38881 gtagtaaccg gccgtgtaga gcgcggtatc atccacgttg gtgacgagat tgaaatcgtc
    38941 ggtctgaaag aaacccaaaa aaccacttgt accggtgttg aaatgttccg caaactgctg
    39001 gacgaaggtc aggcgggcga caacgtaggc gtattgctgc gcggtaccaa acgtgaagac
    39061 gtggaacgcg gtcaggtatt ggctaaaccg ggtactatca ctcctcacac caaattcaaa
    39121 gcagaagtat acgtactgag caaagaagag ggtggtcgtc acactccgtt cttcgccaac
    39181 taccgtccgc aattctactt ccgtaccacc gacgtaaccg gcgcggttac tttggaagaa
    39241 ggtgtggaaa tggtaatgcc gggtgaaaac gtaaccatca ccgtagaact gattgcgcct
    39301 atcgctatgg aagaaggcct gcgctttgcg attcgcgaag gcggccgtac cgtgggtgcc
    39361 ggcgtggttt cttctgttat cgcttaattg aaggatattg ataaatggca aaccaaaaaa
    39421 tccgtatccg cctgaaagct tatgattacg ccctgattga ccgttctgca caagaaatcg
    39481 ttgaaactgc aaaacgtacc ggtgcagttg taaaaggccc gattcctttg ccgaccaaaa
    39541 tcgagcgttt caacattttg cgttctccgc acgtgaacaa aacttcccgt gagcaattgg
    39601 aaatccgcac ccacttgcgc ctgatggaca tcgtggattg gaccgataaa actaccgatg
    39661 cgctgatgaa gctggatttg ccggccggtg ttgatgtaga aatcaaagtc caataattcg
    39721 gactataaaa aatccccaag caatcaatgc ttggggattt tttatgttat gccgagacct
    39781 ttgcaaaatt ccccaaaatc ccctaaattc ccaccaagac atttaggagc accttcttcc
    39841 agcaaaccgc ccaagccatg attgccaaac acatcgaccg gttcccacta tt
//
LOCUS       DAXHNE010000003        37568 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.003, whole genome shotgun sequence.
ACCESSION   DAXHNE010000003 DAXHNE010000000
VERSION     DAXHNE010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 37568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37568
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.003"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            336..980
                     /gene="rplC"
                     /locus_tag="ACFM4Z_000090"
     CDS             336..980
                     /gene="rplC"
                     /locus_tag="ACFM4Z_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFC6020204.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            980..1600
                     /gene="rplD"
                     /locus_tag="ACFM4Z_000091"
     CDS             980..1600
                     /gene="rplD"
                     /locus_tag="ACFM4Z_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFC6020205.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            1597..1911
                     /gene="rplW"
                     /locus_tag="ACFM4Z_000092"
     CDS             1597..1911
                     /gene="rplW"
                     /locus_tag="ACFM4Z_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFC6020206.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANAAKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            1917..2750
                     /gene="rplB"
                     /locus_tag="ACFM4Z_000093"
     CDS             1917..2750
                     /gene="rplB"
                     /locus_tag="ACFM4Z_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFC6020207.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            2757..3035
                     /gene="rpsS"
                     /locus_tag="ACFM4Z_000094"
     CDS             2757..3035
                     /gene="rpsS"
                     /locus_tag="ACFM4Z_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFC6020208.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3044..3373
                     /gene="rplV"
                     /locus_tag="ACFM4Z_000095"
     CDS             3044..3373
                     /gene="rplV"
                     /locus_tag="ACFM4Z_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFC6020209.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            3383..4075
                     /gene="rpsC"
                     /locus_tag="ACFM4Z_000096"
     CDS             3383..4075
                     /gene="rpsC"
                     /locus_tag="ACFM4Z_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFC6020210.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4059..4475
                     /gene="rplP"
                     /locus_tag="ACFM4Z_000097"
     CDS             4059..4475
                     /gene="rplP"
                     /locus_tag="ACFM4Z_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFC6020211.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            4475..4666
                     /gene="rpmC"
                     /locus_tag="ACFM4Z_000098"
     CDS             4475..4666
                     /gene="rpmC"
                     /locus_tag="ACFM4Z_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFC6020212.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            4666..4929
                     /gene="rpsQ"
                     /locus_tag="ACFM4Z_000099"
     CDS             4666..4929
                     /gene="rpsQ"
                     /locus_tag="ACFM4Z_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFC6020213.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5151..5519
                     /gene="rplN"
                     /locus_tag="ACFM4Z_000100"
     CDS             5151..5519
                     /gene="rplN"
                     /locus_tag="ACFM4Z_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFC6020214.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            5531..5854
                     /gene="rplX"
                     /locus_tag="ACFM4Z_000101"
     CDS             5531..5854
                     /gene="rplX"
                     /locus_tag="ACFM4Z_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFC6020215.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            5864..6403
                     /gene="rplE"
                     /locus_tag="ACFM4Z_000102"
     CDS             5864..6403
                     /gene="rplE"
                     /locus_tag="ACFM4Z_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFC6020216.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            6406..6711
                     /gene="rpsN"
                     /locus_tag="ACFM4Z_000103"
     CDS             6406..6711
                     /gene="rpsN"
                     /locus_tag="ACFM4Z_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFC6020217.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            6727..7119
                     /gene="rpsH"
                     /locus_tag="ACFM4Z_000104"
     CDS             6727..7119
                     /gene="rpsH"
                     /locus_tag="ACFM4Z_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFC6020218.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7133..7666
                     /gene="rplF"
                     /locus_tag="ACFM4Z_000105"
     CDS             7133..7666
                     /gene="rplF"
                     /locus_tag="ACFM4Z_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFC6020219.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGAEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            7680..8033
                     /gene="rplR"
                     /locus_tag="ACFM4Z_000106"
     CDS             7680..8033
                     /gene="rplR"
                     /locus_tag="ACFM4Z_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFC6020220.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8051..8569
                     /gene="rpsE"
                     /locus_tag="ACFM4Z_000107"
     CDS             8051..8569
                     /gene="rpsE"
                     /locus_tag="ACFM4Z_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFC6020221.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            8562..8747
                     /gene="rpmD"
                     /locus_tag="ACFM4Z_000108"
     CDS             8562..8747
                     /gene="rpmD"
                     /locus_tag="ACFM4Z_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFC6020222.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            8749..9183
                     /gene="rplO"
                     /locus_tag="ACFM4Z_000109"
     CDS             8749..9183
                     /gene="rplO"
                     /locus_tag="ACFM4Z_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFC6020223.1"
                     /translation="MFLNTIQPAEGATHASRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIAFGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            9195..10505
                     /gene="secY"
                     /locus_tag="ACFM4Z_000110"
     CDS             9195..10505
                     /gene="secY"
                     /locus_tag="ACFM4Z_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFC6020224.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALLVVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            10510..10728
                     /gene="infA"
                     /locus_tag="ACFM4Z_000111"
     CDS             10510..10728
                     /gene="infA"
                     /locus_tag="ACFM4Z_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFC6020225.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            10749..10862
                     /gene="rpmJ"
                     /locus_tag="ACFM4Z_000112"
     CDS             10749..10862
                     /gene="rpmJ"
                     /locus_tag="ACFM4Z_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFC6020226.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            10928..11290
                     /gene="rpsM"
                     /locus_tag="ACFM4Z_000113"
     CDS             10928..11290
                     /gene="rpsM"
                     /locus_tag="ACFM4Z_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFC6020227.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            11310..11705
                     /gene="rpsK"
                     /locus_tag="ACFM4Z_000114"
     CDS             11310..11705
                     /gene="rpsK"
                     /locus_tag="ACFM4Z_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFC6020228.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            11725..12345
                     /gene="rpsD"
                     /locus_tag="ACFM4Z_000115"
     CDS             11725..12345
                     /gene="rpsD"
                     /locus_tag="ACFM4Z_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFC6020229.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            12371..13357
                     /gene="rpoA"
                     /locus_tag="ACFM4Z_000116"
     CDS             12371..13357
                     /gene="rpoA"
                     /locus_tag="ACFM4Z_000116"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFC6020230.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            13381..13749
                     /gene="rplQ"
                     /locus_tag="ACFM4Z_000117"
     CDS             13381..13749
                     /gene="rplQ"
                     /locus_tag="ACFM4Z_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFC6020231.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHEAIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            13896..14609
                     /gene="minC"
                     /locus_tag="ACFM4Z_000118"
     CDS             13896..14609
                     /gene="minC"
                     /locus_tag="ACFM4Z_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFC6020232.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQKTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPNHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            14637..15452
                     /gene="minD"
                     /locus_tag="ACFM4Z_000119"
     CDS             14637..15452
                     /gene="minD"
                     /locus_tag="ACFM4Z_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFC6020233.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            15456..15719
                     /gene="minE"
                     /locus_tag="ACFM4Z_000120"
     CDS             15456..15719
                     /gene="minE"
                     /locus_tag="ACFM4Z_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFC6020234.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            15723..16643
                     /gene="oxyR"
                     /locus_tag="ACFM4Z_000121"
     CDS             15723..16643
                     /gene="oxyR"
                     /locus_tag="ACFM4Z_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HFC6020235.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(16640..16873)
                     /locus_tag="ACFM4Z_000122"
     CDS             complement(16640..16873)
                     /locus_tag="ACFM4Z_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020236.1"
                     /translation="MAIVRQGEATPYWFLLIHYTWHCIGTDENIHSDAKVEYLQDGSF
                     RGDAILKIDDDGNILAYRVVGSGKWRFENNACA"
     gene            17217..17606
                     /locus_tag="ACFM4Z_000123"
     CDS             17217..17606
                     /locus_tag="ACFM4Z_000123"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6020237.1"
                     /translation="MRYTIKTFQTLADETFPSKNFKILSFNGVARPITIVCPTHGVQT
                     VSAGQAFIRSKHGCPACGLVLSTSVLASRGRSVSILDTATGETLSFPSVQAAAKALNT
                     SYGSIRTKLDGRSSPDNLVCNRYKVMP"
     gene            complement(17976..20813)
                     /locus_tag="ACFM4Z_000124"
     CDS             complement(17976..20813)
                     /locus_tag="ACFM4Z_000124"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFC6020238.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     NLDREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLVNVFDLEAKVLANAEVFNFKGEAQPSFALPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQVEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGRVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLEKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGYSFPDMWIVGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKNADSIMLARFPEADGGDIVQTVFEQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            complement(20910..21719)
                     /gene="zupT"
                     /locus_tag="ACFM4Z_000125"
     CDS             complement(20910..21719)
                     /gene="zupT"
                     /locus_tag="ACFM4Z_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFC6020239.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            complement(21900..23156)
                     /locus_tag="ACFM4Z_000126"
     CDS             complement(21900..23156)
                     /locus_tag="ACFM4Z_000126"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFC6020240.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAKGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWRMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEGRKKGTLQIFRQAKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     HFNQTISRIDHNGLRIKTVETETGRFEADAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTRFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(23235..24626)
                     /locus_tag="ACFM4Z_000127"
     CDS             complement(23235..24626)
                     /locus_tag="ACFM4Z_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6020241.1"
                     /translation="MQLFLDNLKAFFETVSGWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(24983..25759)
                     /gene="lpxA"
                     /locus_tag="ACFM4Z_000128"
     CDS             complement(24983..25759)
                     /gene="lpxA"
                     /locus_tag="ACFM4Z_000128"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFC6020242.1"
                     /translation="MTLIHPTAVIDPKAELDSSVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCH
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFTQSTRGIIR"
     gene            complement(25802..26482)
                     /locus_tag="ACFM4Z_000129"
     CDS             complement(25802..26482)
                     /locus_tag="ACFM4Z_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HFC6020243.1"
                     /translation="MERYKNAVGEDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CLQQAFKDRNWLYNSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDATGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINKFRNRLAHHEPICFKGAIKDTNYARNIHQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            complement(26689..27138)
                     /gene="fabZ"
                     /locus_tag="ACFM4Z_000130"
     CDS             complement(26689..27138)
                     /gene="fabZ"
                     /locus_tag="ACFM4Z_000130"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFC6020244.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVS
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            complement(27174..28217)
                     /gene="lpxD"
                     /locus_tag="ACFM4Z_000131"
     CDS             complement(27174..28217)
                     /gene="lpxD"
                     /locus_tag="ACFM4Z_000131"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFC6020245.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEWRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANVYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVHIHRLSEMNKRLK
                     TLEQQLSDGKDTQ"
     gene            complement(28250..28750)
                     /locus_tag="ACFM4Z_000132"
     CDS             complement(28250..28750)
                     /locus_tag="ACFM4Z_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HFC6020246.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(28816..31209)
                     /gene="bamA"
                     /locus_tag="ACFM4Z_000133"
     CDS             complement(28816..31209)
                     /gene="bamA"
                     /locus_tag="ACFM4Z_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFC6020247.1"
                     /translation="MKLKQIASALMMLGISPLAFADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKIGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(31266..32606)
                     /gene="rseP"
                     /locus_tag="ACFM4Z_000134"
     CDS             complement(31266..32606)
                     /gene="rseP"
                     /locus_tag="ACFM4Z_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFC6020248.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVV
                     GGVEKGSPADKAGLQPDDRLTAADGKPIASWQEWANLTRQSPGRKIALTYERAGQTHT
                     ADIRPDTVEQSDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVIRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(32641..33876)
                     /gene="ispC"
                     /locus_tag="ACFM4Z_000135"
     CDS             complement(32641..33876)
                     /gene="ispC"
                     /locus_tag="ACFM4Z_000135"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFC6020249.1"
                     /translation="MPSENASDGIRYKVILIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFQPEYAVVADAEYAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NSAAVLPVDSEHNAVFQVLPRDYTGRLNEHGIASIILTASGGPFLTTDLGTFDSITPA
                     QAVKHPNWSMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(33881..34678)
                     /locus_tag="ACFM4Z_000136"
     CDS             complement(33881..34678)
                     /locus_tag="ACFM4Z_000136"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFC6020250.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSKLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(34681..35427)
                     /locus_tag="ACFM4Z_000137"
     CDS             complement(34681..35427)
                     /locus_tag="ACFM4Z_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFC6020251.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(35483..36040)
                     /gene="frr"
                     /locus_tag="ACFM4Z_000138"
     CDS             complement(35483..36040)
                     /gene="frr"
                     /locus_tag="ACFM4Z_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFC6020252.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(36220..37347)
                     /locus_tag="ACFM4Z_000139"
     CDS             complement(36220..37347)
                     /locus_tag="ACFM4Z_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFC6020253.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRYRRLIHSVRLGGAVLFATASARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWLDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLADCSKMIAEISNGRRMTRERLEENMAKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(37423..37498)
                     /locus_tag="ACFM4Z_000140"
     tRNA            complement(37423..37498)
                     /locus_tag="ACFM4Z_000140"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37463..37465),aa:Thr,seq:cgt)
BASE COUNT         8922 a         8496 c        10118 g        10032 t
ORIGIN      
        1 cacagcctca tcacccgcat cgatttcaac ctgttttgcc gctttgacga actgagcatc
       61 cccgattaca gtcatcaacc atattccggt ttgtcggaga aagatgcata cgctgtgatg
      121 accggatacc gacccgttaa aagagtccga ccctatgccg tctgaaaatt caaaacgctt
      181 cagacggcat attgaagata tttctgatat ttctgttgat atttctttga cttgtcagat
      241 ataatgccga gcttggtaca tttgtgccaa gtttaacttt gtctgaaaga caggccaatc
      301 gtagcctgtc cctttacttt aaaaggaaaa taatcatgac tttaggtctg gttggacgca
      361 aagttggtat gacccgcgtg ttcgacgaac agggtgtttc tgttccggta accgttttgg
      421 atatgtctgc caaccgcgtt acacaagtaa aatccaaaga tactgacggc tatactgccg
      481 ttcaagttac ctttggtcag aaaaaagcca atcgtgtcaa caaagccgaa gccgggcact
      541 ttgcaaaagc aggtgttgaa gccggtcgcg gtttgattga gtttgctttg actgaagaaa
      601 aactggctga attgaaagct ggtgacgaaa tcaccgtttc tatgtttgaa gtcggtcaac
      661 tggtcgatgt aaccggtacc tctaaaggta aaggtttctc cggcacgatt aaacgtcata
      721 acttcggtgc ccaacgtact tcccacggta actcccgttc tcaccgtgtt ccaggctcta
      781 tcggtatggc gcaagacccg ggtcgcgtgt tccccggtaa acgcatggcc ggccaatacg
      841 gcaacaccaa agcaactgtt caaaaattgg aagttgtccg tgttgacgca gaacgccaac
      901 tgctgttggt taagggtgct gttccgggtg cggtcaacag cgatgttgta gttcgtccca
      961 gcgtgaaagt aggtgcgtaa tggaattgaa agtaattgac gctaaaggac aagtttcagg
     1021 cagtctgtct gtttctgatg ctttgttcgc ccgcgaatac aatgaagcgt tggttcatca
     1081 gctggtaaat gcctacttgg caaacgcccg ctccggtaac cgcgctcaaa aaacccgtgc
     1141 cgaagtaaaa cactcaacca aaaaaccatg gcgtcaaaaa ggtaccggcc gtgcccgttc
     1201 cggtatgact tcttctccgc tgtggcgtaa aggtggtcgc gcgttcccga acaaacccga
     1261 cgaaaacttc actcaaaaag taaaccgcaa aatgtaccgt gccggtatgg cgactattct
     1321 gtcccaattg actcgtgacg agcgtttgtt tgcgattgag gcgttgactg ccgaaactcc
     1381 taaaaccaaa gtttttgccg aacaagtgaa aaatctgggt ctggagcaag tgttgtttgt
     1441 aaccaaacag ctcgacgaga atgtttactt ggcttcacgc aacttgccaa acgtgttggt
     1501 tttggaagct caacaagttg atccttacag cttgctgcgt tacaaaaaag taatcatcac
     1561 taaagatgca gttgcacaat tagaggagca atgggtatga atcaacaacg tttgactcaa
     1621 gtgattttgg cacctatcgt ttctgaaaaa agcaacgtat tggctgaaaa acgcaaccaa
     1681 atgacgttta aagttttggc aaatgcagcc aaacctgaaa tcaaagcggc tgtcgagctg
     1741 ctgttcggtg ttcaagttgc ttctgtaact accgttacaa ctaaaggcaa aactaagcgt
     1801 tttggtcgta ctttgggccg ccgcagcgat gttaaaaaag cttatgtaag cttggctgcc
     1861 ggtcaagagt tggatttgga agccgctgct gcagctgcag ataaggaata aacaaaatgg
     1921 caatcgttaa aatgaagccg acctctgcag gccgtcgcgg catggttcgc gtggtaacag
     1981 aaggtttgca caaaggtgca ccttatgcac ccttgcttga aaagaaaaat tctactgccg
     2041 gccgtaacaa taatggtcat atcaccaccc gtcacaaagg cggcggtcat aaacaccatt
     2101 accgtgttgt agactttaaa cgtaacaaag acggcattcc tgccaaagta gagcgtatcg
     2161 aatacgatcc taaccgtact gccttcatcg cactgttgtg ctatgcagac ggcgagcgtc
     2221 gttacatcat tgctcctcgc ggtattcaag ccggtgccgt attggtttcc ggtgctgaag
     2281 ctgccatcaa agtaggcaac accctgccga tccgcaacat tcccgttggt acgactatcc
     2341 actgtatcga aatgaaaccc ggtaaaggtg ctcaaatcgc acgttctgcc ggtgcttctg
     2401 cggtattgct ggctaaagaa ggcgcgtacg ctcaagtccg tctgcgctct ggcgaagtcc
     2461 gtaaaatcaa cgtaaattgc cgtgcgacca tcggtgaagt tggtaacgaa gagcaaagcc
     2521 tgaaaaaaat cggtaaagcc ggtgccaacc gttggcgcgg tattcgtccg accgtacgcg
     2581 gtgttgtcat gaatcctgtc gatcacccgc atggtggtgg tgaaggccgt acgggcgagg
     2641 cccgcgaacc ggtcagccca tggggtactc ctgctaaagg ctaccgcact cgtaataaca
     2701 aacgcacgga taacatgatt gttcgtcgcc gttactcaaa taaaggttaa tttagtatgg
     2761 ctcgttcatt gaaaaaaggc ccatatgtag acctgcattt gctgaaaaaa gtagatgctg
     2821 ctcgcgcaag caacgacaaa cgcccgatta aaacctggtc tcgtcgttct accattctgc
     2881 ctgattttat cggtctgacc attgctgtgc acaacggccg cacccatgtg cctgtgttta
     2941 tcagcgacaa tatggttggt cataaattag gcgaattctc attgacccgt acctttaaag
     3001 gccacttggc cgataaaaag gctaaaaaga aataaggtga atcatgagag taaatgcaca
     3061 acataaaaat gcccgtatct ctgctcaaaa ggctcgtttg gtagctgatt tgattcgtgg
     3121 taaagacgtt gcccaagctt tgaatatttt ggctttcagt cctaaaaaag gtgccgagct
     3181 gattaaaaaa gtattggagt cagctattgc taatgccgag cacaataacg gtgcggacat
     3241 tgatgaactg aaagtggtaa ctatctttgt tgacaaaggc ccaagcttga aacgttttca
     3301 agctcgcgcc aaaggtcgcg gtaaccgcat cgaaaaacaa acttgtcata tcaatgtgac
     3361 agtgggtaac taaggaaaag ctatgggaca aaagattaac cctacaggct ttcgcctggc
     3421 ggtaactaaa gactgggctt caaaatggtt tgctaaaagc accgactttt ctactgtttt
     3481 gaagcaggat atcgatgttc gcaattattt gcgtcaaaaa ttggccaatg cttcggttgg
     3541 tcgagtggtt attgaacgcc ctgcaaaatc tgcacgcatt accattcact ccgctcgtcc
     3601 gggtgtggtt atcggtaaaa aaggtgagga tatcgaggtt ttgaaacgtg acttgcaagt
     3661 cttgatgggt gtacctgttc atgtaaatat tgaagagatt cgccgtcctg agttggatgc
     3721 tcaaattatt gctgacggta ttgcccagca gttggaaaag cgcgttcaat tccgtcgtgc
     3781 tatgaaacga gcaatgcaaa atgcaatgcg ttctggtgct aaaggtatta agattatgac
     3841 ttcaggccgt ctgaatggtg cggatattgc ccgtagcgaa tggtatcgtg aaggtcgcgt
     3901 gccactgcat actttacgtg caaatgtaga ttatgcaacc agcgaagcgc acaccacata
     3961 tggtgtattg ggtctgaaag tttgggttta tacggaaggc aatattaaat cttccaaacc
     4021 tgaacatgag agtaaacaaa gaaaggcagg tagacgtaat gctgcagcca actagactga
     4081 aataccgtaa gcaacaaaag ggtcgcaata ctggcatcgc tactcgcggt aataaggtaa
     4141 gtttcggtga gttcggcttg aaagccgtag gtcgtggtcg tttgactgcc cgtcaaatcg
     4201 aagctgctcg tcgtgcaatg acccgtcata tcaaacgtgg tggtcgtatt tggattcgtg
     4261 tattccctga taaaccgatt actgaaaagc ctattcaagt tcgtatgggt ggcggtaaag
     4321 gtaacgtgga atattacatt gccgaaatta aaccaggtaa agtgttgtat gaaatggatg
     4381 gcgttccaga ggaactggct cgtgaagcat tcgagttggc tgctgccaaa ttgcctattc
     4441 ctacaacctt tgtagtaaga caggtgggtc aataatgaaa gcaaatgaat tgaaagacaa
     4501 atccgttgag cagttgaatg cagatttgtt ggacttgttg aaagctcagt ttggcttacg
     4561 tatgcaaaac gctaccggtc aattaggcaa accaagtgaa ttgaaacgtg tacgtcgcga
     4621 tattgctcgt attaaaaccg ttttaactga aaaaggtgct aagtaatgag cgaaactaaa
     4681 aatgttcgta ctttgcaagg caaagtagta agcgacaaaa tggataaaac cgtaacagta
     4741 ttggttgagc gtaaagtaaa acatccgctg tatggtaaga ttattcggtt atctactaaa
     4801 atccatgccc atgatgaaaa taatcaatat ggaattggtg atgtggttgt tatatcggaa
     4861 tcccgtccat tgtcaaaaac taaatcttgg gttgtcagtg agctggttga gaaagcacgt
     4921 tctatttaag aattaaagca acgtgcttgg aatgggaaac gaagtattgc agcaaattta
     4981 atttgcgtgt aaacttcgtt tcctgtcttt cagtttcttc tggaagtttc ttccctttcg
     5041 gggtccaaga ctggtttact tgaaccgcaa ggtttcattt aataagcagc ggctttgctg
     5101 taagttatct gaaagtggta aattaagttg gttaatttaa aggtaataac atgattcaaa
     5161 tgcagaccat cttagatgtg gctgataact ctggtgcgcg tcgcgtaatg tgtatcaagg
     5221 tattgggcgg atctaagcgt cgctacgctt ctgttggcga tattattaaa gtggcagtta
     5281 aagatgcggc tccgcgtggc cgtgtcaaaa aaggcgatgt atataatgcg gtagttgttc
     5341 gtactgctaa gggtgtacgt cgtcctgatg gtgcgttaat taaattcgat aacaatgccg
     5401 ccgtgttact gaataataaa cttgaacctt tgggtactcg tatctttggt ccggtaaccc
     5461 gtgaattgcg tactgagcga tttatgaaaa tcgtttcatt ggcacctgaa gtattataag
     5521 gaatggcacg atgaataaaa tcattaaagg cgatagggtt gtagtaattg ctggtaagga
     5581 taaaggtaag cagggtcaag tagttcgagt gttgggtgat aaagttgttg ttgagggcgt
     5641 taatgttgta aaacgccatc aaaaacctaa tccaatgcgt ggcattgagg gcggtattat
     5701 tactaaagaa atgcctttgg atatttctaa tatcgcaatc ctgaatccgg aaactaataa
     5761 agcggaccgt gttggtatta agctgattga aaatgaaggc aaagttaaac gcgttcgttt
     5821 cttcaaatca aatggctcta tcattggggc ataaggagat aacatggctc ggttgagaga
     5881 gttttataaa gagacagttg ttcctgaatt ggttaaacaa tttggttaca aatcagtaat
     5941 ggaagtcccg cgtattgaaa aaattacctt gaatatgggt gtgggtgagg ctgttgctga
     6001 taaaaaagtt atggaacatg ctgtttccga tttagagaaa attgccggtc aaaaaccggt
     6061 tgttactgtc gcccgtaaat ctatcgcagg ttttaaaatc cgtgataact atccggttgg
     6121 ttgcaaagta acattgcgtc gtgatcaaat gtttgaattc ttggatcgtt tgattactat
     6181 tgcattacct cgcgtacgtg acttccgtgg tgtgagcggt aaatcatttg atggccgtgg
     6241 caattacaat atgggtgttc gtgagcaaat tatttttccg gaaattgaat acgataaaat
     6301 tgatgctttg cgtggtttga atattactat tactactaca gcaaaaaccg atgaggaagc
     6361 gaaagcttta ttgtcattgt ttaaatttcc gttcaaagga taatcatggc taagaaagca
     6421 cttattaatc gtgatctgaa acgtcaagct ttggctaaaa aatatgcggc taaacgcgcg
     6481 gcaattaaag cggtaatcaa tgattcgaat gcaactgagg aagagcgttt tgaggctcgt
     6541 ttgaggtttc aatccattcc tcgtaatgcg gcacctgtgc gtcaacgtcg tcgttgtgct
     6601 ttgacaggtc gccctcgtgg tactttccgt aaatttggtt tgggtcgtat taaaatccgt
     6661 gaaatcgcca tgcgtggcga aattccgggt gttgttaaag ccagctggta ataggagtaa
     6721 ttaagaatga gtatgcatga tcctatttcc gatatgttga ctcgtatccg caatgcgcaa
     6781 cgtgctaata aagcagcagt tgcaatgcct tcttcaaaat taaagtgtgc tattgcaaag
     6841 gtattgaaag aagaaggata tattgaggac ttcgcagttt catctgacgt aaagtctata
     6901 ttggaaattc aattaaaata ctatgcaggt cgtcctgtaa ttgaacaaat caagcgtgta
     6961 tctcgccctg gtttgcgtat ttataaagcg tctagtgaga ttccaagtgt tatgaatggc
     7021 ttgggtattg ctattgttag tacttctaaa ggtgtaatga ctgatcgtaa agcacgttct
     7081 caaggtgttg gtggtgagtt gttatgcatt gtagcctagt ggaggaaaag aaatgtcacg
     7141 tgtcgcaaaa aacccagtga ctgttcccgc tggtgtagaa gtaaaatttg gagcagaggc
     7201 attagttatt aagggtaaga acggtgaatt gtcttttcct ttgcattctg atgtagccat
     7261 tgaatttaat gatggcaaat tgacttttgt tgcgaataac agcagtaaac aagcaaatgc
     7321 aatgtctggt actgctcgcg cattagtcag caatatggtt aaaggtgttt cagaaggttt
     7381 tgagaaaaga ttgcaattga taggtgtggg ttatcgtgct caagcacaag gtaaaatctt
     7441 gaatctgtct ttgggttttt ctcatccgat cgtatatgaa atgcctgaag gtgtctccgt
     7501 tcaaactcct agccaaacag agattgtttt aaccggctcg gataaacaag ttgttggtca
     7561 agttgctgct gagattcgtg cgttccgtgc tcctgagcct tataaaggta aaggtgttcg
     7621 ctatgtaggc gaagtagtgg taatgaaaga agccaagaaa aaataattga ggttcactaa
     7681 tggataaaca tacaacccga ctccgtcgtg cacgcaaaac ccgtgctcgt attgcggact
     7741 tgaaaatggt aagattatgt gtgttccgaa gcaataatca tatttatgct caagtaatta
     7801 gtgctgaagg tgataaagta ttggctcaag cctctacatt ggaagctgag gtgcgcggta
     7861 gtctgaaatc tggaagcaat gttgaagcag ctgcaatagt tggtaaacgt atcgctgaga
     7921 aggctaaagc agcaggtgtt gaaaaagttg cttttgaccg ttcaggtttc caatatcacg
     7981 gtcgtgtgaa agctttggct gaagctgcac gtgaaaatgg tttaagcttc taatatttgg
     8041 agactttcag atggcaaaac atgaaattga agaacgcggt gacggcctga ttgaaaagat
     8101 ggtcgcagtt aatcgcgtaa ctaaagtagt taaaggtggc cgtatcatgg ctttctcagc
     8161 actgactgtt gttggtgatg gtgatggtcg cattggtatg ggcaaaggta aatcaaaaga
     8221 agtaccagtt gctgttcaaa aagcaatgga tcaagctcga cgctctatga ttaaagtacc
     8281 tttgaaaaac ggtactattc atcatgaggt tattggccgt catggtgcta ctaaagtatt
     8341 tatgcagcct gctaaagagg gtagtggcgt aaaagccggt ggacctatgc gtttggtttt
     8401 tgatgctatg ggcattcata atatctccgc caaagtgcac ggatctacta acccatataa
     8461 tatcgtacgt gcaacattag atggtttgtc taagttgcat actcctgctg atatcgcagc
     8521 caaacgtggc ttgacagtgg aagacatttt gggagttaac catggctgaa caaaaaaaga
     8581 ttagggttac attggttaaa agcctgattg gtacaattga atctcatcgt gcatgtgctc
     8641 gcggtttagg tttgcgccgc cgtgaacata ctgtagaggt tttggatacc cctgaaaacc
     8701 gtggcatgat caataaaatc agctacttgt tgaaagtgga gtcttaatat gtttttgaat
     8761 actattcaac ctgctgaggg tgctactcac gctagtcgtc gtgttggacg cggtattggt
     8821 agcggcttgg gtaagacagg tggtcgtggt cataaaggtc aaaaaagccg ttctggtggg
     8881 tttcataagg tgggtttcga gggtggtcaa atgcccttgc aacgacgcct ccctaaaaga
     8941 ggttttaaat ctttaacagc atcagctaat gcacagcttc gtttaagtga actggaatca
     9001 attgctgtta atgagattga tattttggtc ttaaagcaag cgggtctgat tgcatctaca
     9061 gtctctaatg ttaaagttat tgcttttggt gaaatttcta aggcagttgc tttgaagggt
     9121 attaaagtta ccaaaggtgc gagagctgct atcgaggctg ttggtggtaa gattgaaatg
     9181 taaggtttaa tattgtggct aatcaacaaa cgtcatcagg ttcatccaaa tttggagatc
     9241 ttaagaaacg tcttttgttt ctatttggag cattgattgt ttttcgaatt ggtgcccata
     9301 tacccgtacc tggagttgat gctgttgctt tagctaaatt atacgaaagc gctggaaacg
     9361 gcatcctggg aatattgaat atgttttccg gtgggtcgtt agagcgcttt agtatatttg
     9421 caataggaat tatgccatat atttcagctt ctattattgt acagctcgct tctgaaattt
     9481 tgccatcatt gaaggcttta aaaaaagaag gggaggctgg tagaaaggta attacgaaat
     9541 atactaggta tggtactgtt ttgttagcaa ttcttcaaag tctaggtgtt gcatctttcg
     9601 tatttcagca aggaattgtt gtaacaagtt catttgagtt tcatgtttcc acggtagttt
     9661 ctttggtaac gggaaccatg tttcttatgt ggcttgggga gcaaattact gaaaggggta
     9721 tcgggaacgg tatttcttta atcattacgg caggtattgc ttcaggtatt ccttcgggta
     9781 ttgcaaagct ggttacactg acgaaccaag gttctatgag catgcttacg gcgttgcttg
     9841 ttgtatttgg tgccttatta ttaatttatt tagttgtata ctttgaaagt gcacagcgga
     9901 agattcctat tcattatgca aaacgccagt ttaatggtag ggcgggtagt caaaatacgc
     9961 atatgccttt caagttgaat atggctggtg ttattccccc aatttttgct tccagtatta
    10021 ttctatttcc atctactctt ttaggttggt ttggttcggc tgatacaaat agtgttttgc
    10081 acaaaatagc tggattgtta caacacggtc aattgctgta tatggcttta tttgcagcga
    10141 cagttatttt cttttgttat ttttatacgg ctttggtttt tagccctaaa gaaatggcag
    10201 agaatttaaa aaagagtggt gcttttgttc ctgggattag acctggtgag cagacctcta
    10261 ggtatttaga aaaagttgta ttacgtttga cattgtttgg agctctttat attacaacta
    10321 tttgtttaat tccagagttc ttaactacgg ttttaaatgt acctttttat ttgggtggca
    10381 cgtctttgtt gattctagtt gttgtaacga tggattttag tacacaaata aattcgtata
    10441 ggcttactca acagtatgat aagttaatga ctcgttcaga aatgaaatca ttttctcgga
    10501 aatagaatta tggcgaaaga agatactatc caaatgcaag gtgaaattct tgaaacttta
    10561 cctaatgcaa catttaaagt aaaacttgag aatgaccata ttgtattggg tcatatttct
    10621 gggaagatgc ggatgcatta cattcgtatt tctccgggag ataaggtcac agtagagctg
    10681 acaccttatg atttaactag ggctcgaatc gttttcagag caagataaac caataaaagg
    10741 aaaataaaat gcgtgtacaa ccatctgtta agaaaatttg ccgaaattgc aagattattc
    10801 gtcgaaatcg tgtagttcgt gtaatttgta ctgatctccg tcacaaacag cgtcaaggtt
    10861 aatggaatat ttcttttaat gtgattctgt gatatagtga cacactttgc cctaaaaagg
    10921 aaaaaatatg gctcgtattg caggggtaaa tatccctaat aacgcacaca tcgtaattgg
    10981 tcttcaggct atttacggta ttggtgctac tcgtgctaaa ttgatttgtg aggctgcaaa
    11041 tattgcgcct gatactaaag caaaagattt ggacgagact caattagatg ctttgcgtga
    11101 ccaagttgcc aagtatgaag tagaaggtga tttgcgtcgt gaagtaacta tgagtatcaa
    11161 gcgattgatg gacatgggct gctatcgtgg cttccgtcat cgtcgcggct taccatgccg
    11221 cggtcaacgc actcgtacaa atgcgcgtac ccgcaaaggt ccgcgtaaag cgattgctgg
    11281 taagaaataa attttaagga attttattaa tggctaaagc aaacacagct tcacgtgtac
    11341 gtaaaaaagt acgtaaaacc gtgagtgagg gtattgtgca cgttcatgca tctttcaaca
    11401 ataccatcat tacaatcact gaccgtcaag gcaatgcgtt gtcttgggct acctctggcg
    11461 gcgctggttt taaaggttct cgtaaaagta caccatttgc agcacaagtt gcagcagaag
    11521 cagctggtaa agttgcccaa gagtatggcg ttaaaaattt agaggttcgt attaaaggtc
    11581 caggtccagg tcgcgaatcc tctgtacgtg ctttgaatgc tcttggtttc aagattacca
    11641 gcattactga cgttaccccg ttgcctcata acggttgccg tccgcctaaa aaacgtcgta
    11701 tttaatattg gagtgatttg aaacatggca cgttatattg gccctaaatg taagttggca
    11761 cgtcgcgaag gtacggattt gtttttgaag agtgcgcgcc gctctttgga ttctaaatgt
    11821 aaaattgatt ccgctcccgg tcagcatggt gcaaaaaaac cgcgtttgtc agactatggt
    11881 ttgcagttgc gtgaaaaaca aaaaatccgc cgtatttatg gcgtattaga acgtcagttc
    11941 cgtcgttatt tcgcagaagc tgatcgtcgt aaaggttcta ccggcgagtt gctgttgcag
    12001 ttgctggaat ctcgtttgga taatgtcgtt tatcgtatgg gtttcggttc tacccgagct
    12061 gaagcaagac agcttgtttc tcataaggcg atagttgtga atggacaagt tgtcaatatt
    12121 ccttctttcc aagtgaaagc tggtgatgtt gtctcagttc gtgaaaaagc caaaaaacag
    12181 gtacgtattc aagaagcatt gggtttggca actcaaatcg gcttgccggg ttgggtttct
    12241 gtagatgcgg ataaacttga gggtgtgttc aaaaacatgc cggatcgctc ggaattgacc
    12301 ggtgatatta atgaacagct ggtggtagag ttctactcta aataatgcta gctcagtgag
    12361 ggacagttaa atgcagaata gcacaaccga atttttgaaa cctcgtcaaa ttgatgtaaa
    12421 tactttttct gcaactcgtg caaaagtatc tatgcagcca tttgaacgtg gtttcggtca
    12481 taccttaggt aatgctttgc gccgtatctt actgtcatcc atgaatggtt ttgctcctac
    12541 tgaagtagct attgccggtg tattacacga atattctact gttgatggta ttcaggaaga
    12601 tgttgttgac attttgctga atattaaagg tattgtgttt aaactccatg gtcgtagcca
    12661 agttcaactt gtgttgaaga aatcaggttc aggtgtcgta tctgccggtg atattgagtt
    12721 gccgcatgat gtagaaattc tgaatcctgg tcatgtcatt tgtcatttgg ctgataacgg
    12781 tcaaattgag atggaaatta aagtagagca aggtcgtggt tatcaatctg tttcaggtcg
    12841 tcaggtagtt cgtgatgaga accgtcagat tggtgcaatc cagttggatg cgagcttttc
    12901 gcccatcagc cgtgttagct ttgaggttga acctgcacgt gtagagcagc ggacggatct
    12961 tgataagttg gttttggata tcgaaaccga cggttctatt gatcctgagg aagctgtacg
    13021 cagtgcggca cgtattttga ttgatcagat gtctattttt gctgatttgc agggtacgcc
    13081 tgtggaggag gttgaagaaa aagcacctcc tatcgaccct gttcttttgc gtccggtgga
    13141 tgatctggaa ttgacagtac gttcagctaa ttgtttgaaa gctgaggata tttattatat
    13201 tggcgatttg attcaacgca ctgaaaccga gcttcttaaa acgccgaatt tgggacgtaa
    13261 atctttgaat gagattaagg aagtattggc atctaaaggt ttgacactgg gttctaagtt
    13321 ggaagcatgg ccacctgtag gcttggaaaa gccttaatga agaattaaag gataattgat
    13381 atgcgtcatc gtaatggcaa tcgcaaatta aaccgtacca gcagtcatcg tgctgcaatg
    13441 ctgcgtaata tggcgaattc attattgact cacgaagcta ttgtaacaac tctgcctaag
    13501 gccaaggaat tgcgccgtgt agtagagccg ttgattacat tgggtaaaaa gccgtcattg
    13561 gcaaaccgcc gtttggcatt tgaccgtact cgcgaccgtg atgttgtagt aaaactgttt
    13621 ggcgatttgg gtcctcgttt tactgctcgt aacggtggtt atgttcgggt gttgaaatac
    13681 ggattccgta aaggtgataa tgcacctctg gcactggttg aattggttga caaaccggct
    13741 gctgagtaat tttagtcata taacgccatc tgccgaaaag caggtggcgt tatttttgca
    13801 atatctgata ggtaataggg tattggctat catgtttaaa atattaattg aatagctaag
    13861 gtttgcgcgg taaacttaca tcattaaaaa attctatgat ggtttatata atgaatgctt
    13921 tcgatataaa gtcgacaaag atggacgtat tgtctatatc tttgcatacg tcagacttgt
    13981 ttgatttgga agatgtgctg gtcaaattgg gcaagaagtt tcaagagtct ggtgttgttc
    14041 catttgtgct ggatgttcaa gagtttgatt atcccgagtc tttggatctt gctgcattgg
    14101 tttcgttgtt ttcaaggcat ggtatgcaaa ttttgggtct gaagcattct aatgaacgtt
    14161 gggctgctgc ggctatgaag tatcatttgc tgttttgtct gtctcattcg gaaaatgtta
    14221 aagaactggg tcaggttgag gtgcagaaaa cggaggatgg tcagaaagca aggaaaacag
    14281 tattgattac atcccctgtc cgtaccggtc agcaggttta tgccgaagat ggcgatttga
    14341 ttgttacggg ggcggtcagc cagggggcgg aattgattgc ggatggcaat atacatattt
    14401 atgcgccgat gagggggcgt gctttggccg gtgccaaggg tgatacttct gcccgcatat
    14461 ttatccactc catgcaggca gaactggttt ctgtggcggg tatttaccgt aattttgaac
    14521 aggatttgcc gaaccatctg cacaagcagc cggtacagat attgttgcag gataaccgat
    14581 tggttatcag tgcaattggc tcagagtaat tgtttgatat ttaaaaagga aatattgtgg
    14641 caaaaattat tgtagtaact tcaggtaagg gcggtgtcgg taaaacgact accagtgcca
    14701 gtattgcgac aggtttggca ttacgcggat ataaaactgc ggtaattgat tttgatgtgg
    14761 gtttgcgtaa cctcgacctc attatgggtt gcgagcgtcg tgtcgtttat gacctgatca
    14821 atgtcattca gggggaggcg acgctcaacc aagctttgat taaagataaa aattgtgaaa
    14881 acctgtttat tttgccggct tcccagactc gggataaaga cgctttgaca cgcgagggcg
    14941 tagaaaaagt gatgcaggag ctgtccggca agaaaatggg ctttgagtat attatttgcg
    15001 actctcctgc cggtattgag cagggtgcat tgatggcgtt gtattttgct gatgaagcca
    15061 ttgtaacgac caatcctgag gtttccagtg tgcgtgactc cgacaggatt ttgggaattt
    15121 tgcaaagcaa atcccataag gcagagcaag gcggttcggt taaagaacat ctgttgatta
    15181 cgcgttattc tcccgaacgt gtggcaaaag gcgaaatgct gtctgtacag gatatttgcg
    15241 atattctgca tattcctttg ctgggtgtga ttcctgaatc ccaaaacgtc ttgcaggcat
    15301 ccaattccgg agaaccggtc atccatcagg acagcgtggc ggcttccgag gcatataagg
    15361 acgttattgc ccgtcttttg ggcgagaacc gtgaaatgcg tttcttggaa gctgagaaaa
    15421 aaagcttctt caaacgtctg tttggaggat aaggtatgtc attaatcgaa tttttattcg
    15481 gcagaaagca gaaaacggca accgttgccc gcgaccgcct tcaaatcatc attgcccaag
    15541 agcgcgccca agaaggtcag gctccggatt acctgccgac tttacgtaaa gagttgatgg
    15601 aagtcctgtc caaatatgtg aatgtttcat tagacaatat ccgtatttcc caggaaaaac
    15661 aggatggtat ggatgtgctt gagttgaaca ttactttgcc ggaacagaaa aaggtatagg
    15721 acatgacctt aaccgaattg cggtacatcg tcgcagtcgc ccaagaacgt catttcggca
    15781 gggcggcgcg gcgttgtttt gtcagccagc ccactttgtc tattgccatt aagaaattgg
    15841 aagaagagct tgccgtctct ttgtttgacc ggagcagtaa cgatattatt acgaccgagg
    15901 cgggggaacg tatcgttgca caggcgcgta aggtattgga agaggcggag cttatcaggc
    15961 atttggcaaa tgaagaacaa aacgagctgg agggtgcgtt caaactcggg ctgattttta
    16021 cggttgcgcc gtacctgctg ccgaaactga ttgtttcgtt gcgccgtact gcaccgaaaa
    16081 tgcctttgat gttggaagag aattacacgc atactttgac cgagtcgctc aaacgcgggg
    16141 acgttgatgc gattatcgtt gccgaaccgt ttcaagagcc gggcattgtt accgaaccct
    16201 tgtatgacga accgtttttc gtgattgtcc cgaaagggca ttcatttgag gaactggatg
    16261 ccgtttcgcc acggatgctg ggtgaggagc aggttttgct gctgacggaa ggcaactgta
    16321 tgcgggatca ggtactctca agctgttccg aattggcggc gaaacaacgt atacaggggt
    16381 tgaccaatac attgcagggc agctcgatta atacaatccg ccatatggtt gccagcggtt
    16441 tggcaatcag cgtgttaccg gcaaccgcgc tgaccgaaaa cgatcatatg ctgttcagca
    16501 ttattccgtt tgagggtacg ccgccaagcc ggcgggtcgt attggcgtac cgccgcaatt
    16561 ttgtccgtcc gaaggcgttg tcggcgatga aggcggcgat tatgcagtcg cagcttcacg
    16621 gggtaagttt tatccgcgac taggcgcagg cattgttttc aaaacgccat ttccctgagc
    16681 cgacaacacg gtatgccaag atattgccgt catcatcgat tttgagtata gcatcgccac
    16741 ggaaactgcc gtcctgaaga tattcgactt ttgcatcact gtgaatgttt tcatcagtgc
    16801 cgatgcaatg ccatgtatag tggattaaca aaaaccagta cggcgttgcc tcgccttgcc
    16861 gtactattgc caaggcgtac cagcctgatt tgttgatgtg tgtactactg catgggttgt
    16921 taagccctgc aagtgattta agtgtgaaga ttgagtaatg cccactagtc gcaggcgcgg
    16981 ggggcaggtg ctaagtcatt ggttatctcc ttactatcta tctaactaca gggtgtatta
    17041 tgcgcctcct tgttgttttg tgcaagtggt tttctgataa tttatgtaaa gatttggtaa
    17101 agacgtatag cctttttctt tgcattgtgc ttgcgcaggg tatcaggtag gtctcaaaaa
    17161 atttgaaaat ttttatgttt tcgtataaaa tcttttgcac ttatacggag tgagttatgc
    17221 ggtacaccat taaaacattt caaacccttg ctgatgaaac tttcccctct aagaacttca
    17281 aaattctttc gttcaatggg gtggcacgac ctataaccat tgtttgccct acacacgggg
    17341 tgcagactgt ttctgcgggg caagcattta tccgttccaa acacggctgt cctgcctgtg
    17401 gtttagtttt atcaacctcc gttttggcct ctaggggtcg gtcagtctcc atcctcgaca
    17461 ccgccacagg cgaaacactc tcgttcccca gcgtacaagc tgccgccaaa gccctaaaca
    17521 cctcctacgg ctctatacga accaagctcg acggacgttc gtcccccgac aaccttgtct
    17581 gtaacaggta caaagtaatg ccgtaatctc gaaacttcgt gctataataa actcctagct
    17641 tttgcaggag tttgtttttt atggatttaa atgtcgaaat caagaatttt ggcaaggttg
    17701 cccatgctag ggtttccatt cgcccgctta ccgtcatcac gggttgcaac ccacactcgc
    17761 gttccccagc gttactgccg caggcactgc tttgggtgtg cattttcagc aaattaacca
    17821 caggctcaag ggtcgaacgt cctgtcagga ggtgttgatt tctacaccga ttatactgtc
    17881 tgcggcttcg tcgccttgtc ctgatttttg ttaatccact ataaaagagg ccgtctgaaa
    17941 aacatttttc agacggcctt gtttattcaa tcaaatcagt ctttcaactt cgccaactga
    18001 ttttgaactt ttgccatttt gtcttccaat tccgccaaat cggctttgtc tttttccacc
    18061 agatgcgcag gggctttttc ggtgtagccg ggtttggaga gtttggcgtt gagtttgtcc
    18121 aaggcttttt gcagcttctc ggcttctttg ctcaaacggg cggtttcggc ggctttgtcg
    18181 atttcgactt tcaacatcag gcgcgcgccg ttgcagacgg cgacgggcgc gtcttcgctt
    18241 tcgggtaggg cggcgacttg ctgcgcttcg gtcaggcggg tcatcatcgg caggtatttg
    18301 aggtagtctg ccaagtcgtc cgcgctttcg acaaacagcg gagctttgac attaggctgg
    18361 atacccattt cgccgcgcag gttgcggact gcgccaatca aatcctgcaa tacggtcatt
    18421 tgctcgaata ccgtctgaac aatatcgccg ccgtcggctt cggggaagcg ggcgagcatg
    18481 atgctgtcgg cgtttttcgc gtcgcacata ggagcgacgg tttgccacag ttcttcggta
    18541 atgaacggga taatcgggtg cagcaggcgc agggcggctt cgagtacgcg caataaggta
    18601 tggcgtgtgg cgcgttggcg gctggcgcag ccggtttgaa gctgcacttt ggcaagttcc
    18661 agataccagt cgcaatagtc gttccacacg aagctgtaca gggtttccgc cgccaaatca
    18721 aagcggtagg tttcgtaggc ttgcgttact tgctcgatgg tctgattcag acggcctaca
    18781 atccacatat cggggaagga gtagccgcgc ggttcggcag cggttgcgcc gtaaccgcag
    18841 tcttggtttt cggtgttcat caagacgaag ttggtggcgt tccagatttt gttgcagaag
    18901 ttgcggtagc cttcggcgcg tttgaagtcg aagttgaccg aacgccccaa gctggcgtag
    18961 ctcgccatag tgaagcgcaa agcgtccgcg cccatactcg gaatgccttc ggggaagagt
    19021 tttttcgtgg cttcttccac tttcggcgcg gtttcgggtt tgcgcaggcc ggtggtgcgt
    19081 tttaccagca gtttttccaa gccgatgccg tcgatcaaat ccacagggtc aatgacgttg
    19141 ccttcggatt tggacatttt tttgccttcg tggtcgcgca cgatgccgtg gatatacacg
    19201 tctttaaacg gtactctgcc ggtgaagtgg gtggtcatca taatcatacg cgccacccag
    19261 aagaagatga tttcgtagcc ggtaaccaag acgttggacg gcaggaaggc tttgagttcg
    19321 tcggtttcag acggccagcc gagcgtggag aacggcacga gcgcggagga gaaccaagtg
    19381 tccaatacgt cttcttcgcg ggtcaagcct gttttgccgg cttgtttttc ggcttccacc
    19441 tgattgcggg cgacgtaaac attgcctgct tcgtcgtacc acgcggggat ttgatgcccc
    19501 caccacagtt ggcgcgagat gcaccagtct tggatgttgt tcatccattg gttgtaggtg
    19561 ttgacccagt tttcggggat aaagcgtacc gcgccgctgt cgacggcttt tttggctttg
    19621 tcggcgaggc tcaagccttt gaattcgctg tcaggctcgc cgccgtttgg cgtagcggac
    19681 atggcgacaa accattggct ggtcagcatc ggctcaatca ctgagcctgt tcggtcgcct
    19741 ttcggcgtca tcagcgtgtg cgctttgatt tcgaccaaga aaccttgttc ctgcaaatcg
    19801 gcaaccattt gtttgcgcgc ggcaaagcgg tctaagcctg cgtatttttc aggcagggca
    19861 aagcttggtt gcgcttcgcc tttgaagttg aacacttcgg cgtttgccaa tactttggct
    19921 tccaagtcga acacattaac caagcgcgta tcgtggcgtt tgccgacttc gtagtcgttg
    19981 aagtcgtgcg caggcgtgat tttcacgcag ccggtgccga agtctttttc aacgtattcg
    20041 tcggcaatca cgggaatggt gcggccggtc agcggcagga ttaattcttt gccgattaag
    20101 tgggtataac gttcgtcttc gggattgacg gcaacggcaa cgtcgcccaa cagcgtttcg
    20161 ggacgggtgg tggcgacgat gacggcttcg gcaggattgt ccgccagcgg atagcggatg
    20221 tgccacatag agccttgttc ttccacgctt tccacttcca aatcagacac tgcggtaccc
    20281 aataccggat cccagttcac caagcgtttg ccgcgataaa tcaagccctg ttcatacagg
    20341 cgtacgaaca cttcggttac ggtttcggcg cgtacatcgt ccattgtgaa atactcgcgc
    20401 gtccagtcgg cagaacagcc cacacggcgc atctgctgcg taatcgtgcc gccggaaact
    20461 tctttccatt cccacacttt ttccaagaat ttttcacgat ccaagttatg acgggacacg
    20521 ttttgcgcgg caagctgacg ctcgaccacg atttgcgtgg cgatgcccgc gtggtcggtg
    20581 ccgggaatcc aggcggtgtt gcagcctttc atacggtagt agcgggtcag gccgtccata
    20641 atggtttggt tgaaggcatg acccatgtgc agcgtgccgg ttacattggg cggcggcagt
    20701 tggatggaaa aagacggttt ggtcaaatcc atatcaggtt ggaaatagcc ttggctttcc
    20761 cagttttgat agtgcttgga ttcgatttcg gcgggggagt atttgtctaa catgatggaa
    20821 ctttgtgaaa ttaaggttat ttttgatgtg cggattataa cgcaaaaagg ccgtctgaat
    20881 catttcagac ggcctttggc atacaggttt taaaaatgga acaataccag gctgacggca
    20941 atcaccgcca tgcccattgt caggccgtaa acggtttcat ggccgtctga atagcgtttg
    21001 gcagccggca gcagctcgtc caacgccaaa aacaccatca caccggctat cacgccgaat
    21061 accgaaccaa acacggcagg cgacaaaaac ggctgcaaaa ccaaatagcc caaagccgcc
    21121 cccaacggct cggccaagcc ggatagcaga cacgcccaca ccgttttctt acggctgcgg
    21181 gtggcaaaat aaaccggcgc ggcgatggaa atgccctccg gaatattatg gatggcaatc
    21241 gccaaggcca aaggcatccc gactgctgga ttttccaatg tggcaaaaaa cgtcgccaag
    21301 ccttcgggga aattgtgcgc agtaatcgca aacgccgcca tcatgccgac tcgcgcgata
    21361 tggcggcgtt tgctttcttg aaacgacggg tcttgcgcgt ctaaagtttc atgcgggttc
    21421 ggcaccagac ggtcaatcag cgcaatgccg cccatcccgg ccaaaaatgc catggtcgcc
    21481 gccgcaaacg cgtggtcttt atcataaatt tcagcgaacg cctcgctgga tttactgaaa
    21541 atctccgtca gggaaacata taccatcgca ccgccggcaa acgccaaacc aaacgacaac
    21601 acacgcggat tgggcgtttt ggaaaacatc accaagccac tgcctaatac ggtaaacaaa
    21661 ccggcagcca atgtgatgga aaaggcaacg gccaaattgg acatcgaaaa atcgggcatg
    21721 agaaaacctg cgctaaaagc tgggacaggt tcagactaac actttttaat gtatatgata
    21781 atagttatta tttattttat tgattggata cacggatttt gaaacaaaag gccgtctgaa
    21841 aaatgatttt cagacggcct ttaaatttga aatgccgcta aaccttatcg ctttccagct
    21901 taagcctgat aacgcgacag gctcaaatcg tcgctgcgga tttcggtgtc tttgccgctc
    21961 acgatatcgg cggttaattt tgccgaaccc agcgacatgg tccagcctaa agtaccgtgg
    22021 ccggtattca gaaacaggtt gtcaaagcgg gtgcgaccga ttaacggcgt gctgtcgggc
    22081 gtcatcggtc tgaggccgct ccagaacgat gcttggctca aatcgccgcc ttccgggaac
    22141 aagtcgttga cgaccaaagc caaggtttcg cggcgttttt cgggcagttt gatttcgtag
    22201 cccgacaatt ccgccatacc gccgacgcgg attctgttgt caaagcgcgt gatggcgact
    22261 ttgtagcttt catctaaaac ggtggacacc ggtgcgccgt cggaatttgt gaccggcagg
    22321 gtcaaggaat agcctttgac gggataaatg ggcagattga gatccaactg cgccaaaacc
    22381 gtcctgctga aacaaccgag cgcgcagaca acggcatctg cttcaaaccg ccctgtttcg
    22441 gtttcaacgg ttttgatgcg cagcccgttg tggtcgatgc ggctgatggt ttggttgaaa
    22501 tgaaaccgta cgcccttttc ctgacacaat ttgtataggt tttcagtgaa gaggcggcag
    22561 tcgccggttg catccgcagg caggtgcagg ccgccggcaa ttttggcggt aacgcgtgcc
    22621 agcgcaggct cgaattctgc gcattcttcg ggcttcagac ggcggtacgg cacgccgtag
    22681 cgttccaaaa cggcaatgtc ttgttctgca gcttcaactt ctttagcctg acggaaaatc
    22741 tgcaacgtcc cttttttgcg cccttcaaaa ttcatgccgg tttgcgcttc aaaacggcgg
    22801 aacatttcac ggctgtattc ggaaatcctg accatgcgct ctttattggt ttgatagtgc
    22861 gctgccgtgc agttttgcag catccgccac agccattcga tttgatacag gctgccgtcg
    22921 gggcgaaaca gcaaaggcgg atggctttta aacagccatt tcagtgcttt ggtcggaata
    22981 ccgggtgcag cccaaggcgt ggtatagccg taagaaagct ggcctgcatt ggcaaaactg
    23041 gtttccatcg ccacaccctt ggcgcggtcg atgaccgtta cttcatgtcc ggcctctgcc
    23101 agataccacg cggaagacac gccggcaaca cccgcaccta aaacaagcac tttcatgttt
    23161 ctccctccga ctttttcaaa acagacttaa tatgccgtgc cgtctgaata ttcggattca
    23221 gacggcctcg gatattaatg cggcaattcg ccgtttgtga ttttttgttt gaagtcgcgc
    23281 gtttcattga cgatgacttt cgccatcaat aaaagtgcaa tcaggttggg caatgccatc
    23341 aagccgttga atgtgtccga agccagccac accaaatcaa ggctcaacac ggtacccagc
    23401 ataacggaag aaacataacc gacgcggtac aaaccggcaa atttctcgcc gaaaacatac
    23461 accgcgcatt tttcgccgta atagcaccag cccaaaatgg ttgagtaggc aaagaaaatc
    23521 aggccgatgg taacaatcca gccgccgatg ccgggcagca ttttttggaa tgtgacggtt
    23581 gtcagtgccg cgccgctcac ttcaggtttg acaaactcgc cgcccgcgcc gagcagtccc
    23641 attaccaaca cgatgccggt aatcgagcaa acgacgatgg tatccaaaaa cgtaccggtc
    23701 atagaaacca aggcctgacg gacgggatgg tcggttttcg cggctgcggc ggcaatcggt
    23761 gcagaaccca tgcccgcctc attggagaac acgccgcgcg ccacgccgta gcggatgacc
    23821 gtaccgatag cgccgcccgc cactgcctgc gcgctgaacg catcggagaa aatcagcttg
    23881 acggcaggca tcagtgcatc ggaattaatc gcgataatgg aaagaccgcc caacacataa
    23941 aacaccgcca tagcaggcac gatgaaagaa gcggctttgg cgatgccttt aataccacct
    24001 aaaacgacaa cggcagtcag aacggtcaac gtaatgccgg tataggcagg ttcgataccg
    24061 aagctggttt gcaccgcctg tgcaaccgag ttggactgca ccgagctgcc gataccgaag
    24121 gaagcgaatg tgccgaacag cgcaaacgcg acggccatcc atttccagtt tttgcccaag
    24181 cctttttcga tgtaatacat cgggccgccg gacatttcgc ctttggaatt gttgacgcgg
    24241 tatttcaccg ccaacacgcc ttcgccgtat ttggtggcca tgccgaaaat ggcggtcatc
    24301 cacatccaaa ataccgcgcc cgggccgccg gttaccaccg cagtcgccac gccggcgatg
    24361 ttacccgtac cgatggtggc ggacagcgcg gtcatcaacg ccgcgaaatg ggaaatatcg
    24421 ccttcgtggc cttcgccgcc ttcatacttc tttgacggca caaatgcctg tttcagcgca
    24481 tagcccaaca tagtgaactg caagcctttc agcataacgg tcagcacaat gccggtgccg
    24541 accagcagca tcaacataac agatccccag acccagccgc tgacggtttc gaaaaaggct
    24601 ttgagattgt ctaaaaacaa ttgcatggct ttctcctttg tctgttttat ttttaaaaca
    24661 ccacttttgt agtgtcctat aatttcagca cagaatatcc aataagacaa tatgttcttt
    24721 tgaaaaatac ttttggtttt ttcgccgaaa gcaggccggt tcaagctgcg gaaattgttt
    24781 gcaattcttt aaaagcagcg gcggaggtca caatgaaatg tccgaatggg gatgtggtcg
    24841 gcggcagaaa tcatcaatgc tgccgactgc catacttctg aaatctacaa aatgatgcat
    24901 cgatcaaaca atataccgct ttaaaaaaac cgatgccgtc tgaaacgctt tcggggtttc
    24961 agacggcatc aaaagggtac ggtcagcgga tgatgccgcg cgtcgattgt gtgaaaaagt
    25021 ctcggaatac ggcaagctcg gcttgggttt cggcgcggcg gaggatgtct gccttggcct
    25081 cttcaaacgg aatgccgcga tggtagaggg ttttgtacac gtctttgacg gcggaaatct
    25141 gttctgcggt aaaaccgttg cggcgcatgc cttcgctgtt gagccccgcc ggttcggcgc
    25201 ggtagcccga tgccataaag tagggcggta cgtctttgtg tacgcctgcg gcgaaggcgg
    25261 tcatagcgta gtcgccgatg tggcagaatt ggaaaaccag cgtgtagccg cccaaaacga
    25321 cgtagtcgcc gatggtaacg tgtccggcaa gcgaggcgtt gttggcgaag atggtgtggt
    25381 tgccgaccac gcagtcgtgc gcgaggtggc agtacgccat aatccagttg tcgtcgccga
    25441 tacgggtttc gccgatgccg gttaccgtgc ctaaattaaa ggtggtgaat tcgcggatgg
    25501 tgttgccgtt gccgataatc agcttggtcg gctcgtcgcg gtattttttg tcctgcggga
    25561 tttcgccgag gctggcaaat tggaaaatgc ggttgttttc gccgatgctg gtgtggccgt
    25621 tgatgacggc gtgcgggccg atttcggtat tcgcgccgat ttggacgttg gggccgataa
    25681 cagtgtacgc gccgactttg acgctggagt cgagttcggc tttggggtcg atgacggcgg
    25741 tcgggtggat gagggtcatg ttttgaatac tccgctgctt gttaagacaa ataatagaaa
    25801 attacagttt gtcgatttca tcgcagaccg caatcacttg atcgctaaaa tggctaaata
    25861 ctgaagcagt atctacatcc atgtaattaa gcagttcaaa aatagattgg tgaatatttc
    25921 tagcgtagtt ggtatctttt attgcacctt taaaacaaat cggctcgtga tgtgccaaac
    25981 gattacggaa tttgttaata ttggaaagtt ctcgaaaaat ccatttttgg ttatattgca
    26041 cacttggtgt agatttgggc ttttttggaa aaactttcat caatactttc cctgtagcat
    26101 caaattgggc atctttccct cctgcaaaca gataccgcca aaaaccgaat cctagtcctg
    26161 caaccaattg gttgtggtca aactttcccc cgctattatt ttgtatttta gataatgcaa
    26221 ccttgattaa ttcagcagat tctttcgtgc catttcttaa aaagcaacct tgatatttta
    26281 atgcgggatt cgtctgtggc tgtatgctgt tatataacca gtttctgtct ttaaacgcct
    26341 gctgaaggca aatgtcaatt ttgtttctta aaacaatctc aaatattgaa acaacatgaa
    26401 aaagttctct tgataaagat aaattcaata aatacaattc agctgctttg tcttccccaa
    26461 cggcattttt ataccgttcc attctgaaat cagaaaaaat actacagcat tccttataat
    26521 ccacgctaac acctgattct ttatttttaa attgacgttt caaactatat ttgatacagt
    26581 taggtaaaac taatccccac cgtgtccctg ggtttgaata aacctaaacc ggtgggtttt
    26641 ctttttatta ggtcgtctga aactttcaga cgaccttttt ccgaacgctc aaaccacgcg
    26701 tttggcgcac ataatcacgg cttcaacggc aacttgcccg tccactttgg cgacggcgtt
    26761 gaatttgccg atgccgcgcc ggctggtcag cagctcgact tcaaagacga gttggtcgcc
    26821 ggggatgact tggcgtttga aacgggcttc gtctatgccg gcgaagaaga agaattcgtt
    26881 ttctttgcgc ccgccttcgc tcaaaatcgc caacgtgccg caggcttgcg ccatcgcttc
    26941 gatgatgagt acgccgggca tcacgggcag gtcgggaaag tggccttgga actggggctc
    27001 gtttatgctg acgtttttaa tcgcggtcag ggttttcatc ggctcgaagg cggtgatgcg
    27061 gtcgagctgg agaaacggat agcggtgggg gatgagtttt tggatgtctt tggcttcgat
    27121 ggggagttgt acgtccatgt ctgtcgtatt ccttgaataa aatcggtttg gatttattgc
    27181 gtgtctttgc cgtctgaaag ctgctgttcc agcgttttga ggcgtttgtt catttcgctt
    27241 aagcggtgga tatggacggc gttgcgcgcc cattctttat gggtggacat cgggaagatg
    27301 ccggcgaggt gtttgccgct ttcggtaatg ctgtgggtga cggacgtgcc gccgccgatg
    27361 gtggttttgt cggcgatttc gatgtgtccg accgtaccga cgccgccgcc gatgatgcag
    27421 tagctgccta tggttacgct gcctgagatg ccggttttgg cggcgatgac ggtgtgcgaa
    27481 ccgattttgc agttgtgtcc gatttggact tggttgtcga ttttggtgcc gttgccgacg
    27541 gtggtgtcgc tcatcgcgcc gcggtcgatg ttggtgttcg agccgatttc tacgtcgtcg
    27601 cccagcgtta ccgcgccggt ttgcgggatt ttaaaccatg aatcgccggc aaaagcgagt
    27661 ccgaaaccgt ccgcgccgat gaccgcgccg ctgtggattt cgacgcgtct gcccagtgtg
    27721 cagccgtaat aaacgacggc gttgggatgc aggacgactt cgtcgcccag tttgcaatcg
    27781 tgttggacga cggcgtttgc caagatgcgg cagccttcgc cgagcacggt gtttgcgccg
    27841 atgtagacgt tcgcgccgat ttcgcagctg gcgggaacgg tcgcgcccgg ttcgacgacg
    27901 gcggtcggat ggatgccgcc gcgcgctttg acgacgggtg aaaacaggcg ggcgactttg
    27961 gcgaaataga gataggggtc gtcggcgaca atcaggttgc gccattcaaa tccgtctgcc
    28021 gctttggcgg aaacgatgac cgcgcccgcg ctgctgtcgt ggacttcggc tttgtatttc
    28081 ggattggcaa ggaagctgat gtgttccgcc tgcgcgtctg cgagcgggcg cacggcggta
    28141 acggaaatgt cctcgccgcg ccattcgccg ccgagccgcg cggtgatttg ggacagggtg
    28201 taggtggccg gaatcatggt tttcctgttc ggtatgccgt ctgaaagggt cagcgggcgt
    28261 tcatttcttt aatgacgctg tcagtaacgt cgtattgggt gttgacgtaa atcacgttct
    28321 gcaaaatgac atcgtaacct tcctgtttgg cgattttgac gatgacgcgg ttggcgtttt
    28381 gctggaggga ggcaaactct tcgttgcggc ggaggttgta gtcttcttca aactgcgcct
    28441 gttttttgcg gaacgctgcg accagcccgc gccatttttc ttcggcttgc gccttttttg
    28501 cgtttctgag tttgccttcg gcaagctgcc tttccaaatc cagaccttcg cgttgcagtt
    28561 tttgcaattc gtcctgacgg gcggaaaatt cgctgtccag cgttttttga atcttgcgcg
    28621 cctgcttgga ttcgaggtag atgcgctcgg tgttgataaa gccgattttt tggaaggtgt
    28681 cggcgtgcgc gcctgcggtg cagcacaaac cgatcagagc cgcggcaaac gcgcgggtca
    28741 aacgggtcat ggtaaaactc cttcgaatgt tgccgcgaaa tgccgtctga agggcttcag
    28801 acggcatttg cgggattaga acgtcgtgcc gagttggaat tggaagcgtt ggatttcgtc
    28861 ttccggtttt ttcttcagcg ggtaggcgta gctgaatttc atcgggccta aaggcgagag
    28921 ccaggtaacc gcgccgccgg cggaatagcg caattcgttg gtaaaggtgg atttatgggt
    28981 attgccggcg ccgtaaatgt tttgaaccct gccgccggtc gcggaactgc tgttgtcgtc
    29041 gtaggttttg ccgtcccaca cgctgcctgc gtcggcaaac aggctcaggc ggacggtgcg
    29101 cgcgtctttc gcgccaggca tcgggaagag cagctcggcg gagacgttgg cttttttgtt
    29161 gccgccgtag ctgatttttt cgccgtattc gtcatacact ttcggaccga gcgtgccgct
    29221 ttcgtatccg cgcaccgaac ccaggccgcc gccgtagaag ttttcaaaga aggggatttc
    29281 tttggttctg ccgtagccgc ccgcaatgcc gacttcgccg ccgagcatca gcgtgaaggt
    29341 tttgcttaag gggaagaacc aggtttggtt gtgggtggcg gagtagtatt gcagtttgct
    29401 gccgggcagg gcgatttcgg cgttcacgcc cgtcaggtag ccgcgcgtcg gccataacgc
    29461 gctgtcggtt ttgttgcgcc cccagccgac ggtacctttg tacagccagc ctttgaagct
    29521 gccgtctgtg ccgtcggttt tgccgtattt cttgataaag tcggcatagt gtttgggcgc
    29581 tttgttgtag gtgttgacgg tcaggtgttc tgccaccaaa ccgaaattca cgcggtcgta
    29641 ttcggtaaca ggcacgctca tgcggatgcc tgcgcctgcc gtggtggttt tatattgttt
    29701 gatgctggtc gatgctttgc gcgggtcgaa ggcttttccg taaacatcgt agcccaggct
    29761 gaccccgtct gccgtgaagt acgggtcggt aaacgacagc gagccgttga gcgtggtttt
    29821 gcttcgtgag gcgcgcaggg cggccgactt gcccgtaccg aacaggttgt cttgggaaac
    29881 gcctgcggac atgaccaggc cggtatcctg tacccagccc gcgctcaagt cgagcgagcc
    29941 ggtggaacgt tcggtcaggc tcatgttcaa atcgactttg tcgggtgtgc cggcaagcgg
    30001 gacggcgtca aactggacat tgtcgaagta gcccaaaagc tcgacgcgct ctttggagcg
    30061 ttgcagcttg gaggtgtcgt aaggcgcgga ttccatttgg cgcaattcgc ggcgcacgac
    30121 ttcgtcgcga gttttgttgt tgccggtgat gtggatttcg ttgacgtaga ttttccggcc
    30181 cggttcgatg tgcaggacga aatcgacggt tttggtttcg gcgtttggca gcggctgtac
    30241 gctgatttcg ctgtatgcgt agcctgccga gcccatgcgg ttctgaatct cacccaaaac
    30301 ggcggtcatc tgctggcgtt cgtaccattt gccgggcttc atggtcagca gcttttccag
    30361 ttcggctttg gggacttcgt tggtgtcgcc ttcgatggag actttgcccc aacggaaacg
    30421 tccgccttcg tggacggtga ttttgatggt ctgcttggtt ttgtcttcgt tggtttggat
    30481 gtcggtatcg aggatgcgga aatcgaagta gccgttgttc tggtagaagt cggttacttt
    30541 ttccatgtct tgggcaaatt tctgctcgtt gaattggttg cttcgtgtca gccatgtcca
    30601 aatgccgcct tcggtcagcg acatctgccg catcagtttg cggtcggaat agacttggtt
    30661 gccttcaaat tcgatgtcgg tgattttggc ggatttgccc tcgtcaatcg tgatgtcgat
    30721 gtcgacgcgg ttgcgggcga gtttggttac tttgggcgtg atttggatat tgagtttgcc
    30781 gcgcccgagg tattcttctt tcaggccggc gactgcctga ttgagtgtcg cctgattaaa
    30841 gtattgcgac tgcgccagcc cgaacgattc gaggtttttc ttaatggcat cgttttgcag
    30901 catttttgcg ccggtgatat tgagcgatcc gatggtgggg cgttcgatga cggtcagcag
    30961 gagctgcccg tccgcagtct cgacgcgtac gtcgtcaaag aaaccggtgg cgtacaggct
    31021 tttgatgatg gcactgccgt gtgtgtcgtt gtaggtgtcg ccgattttga cgggcaggta
    31081 gttgaatacg gtgcttggct cggtacgctg caagccttcg acgcggatgt cttggatggt
    31141 gaagtcggca aatgccaaag gcgatatgcc caacatcatc agtgcggaag caatctgttt
    31201 cagtttcatc gtaagttcct tgtggtgcgg aatgcggttt cagacggcat tccgaaacgt
    31261 aaaatctaac cgagcagccg ggtaacgtcg ttgaagaagg cgaccgccat catcagcatc
    31321 atgagggcaa gcccgaagcg caaaccgatg ttttggacgc gttcgcccaa aggtttgccg
    31381 cgtatccatt cggcagtata aaacacgagg tggccgccgt ccaaaacggg gacgggcagt
    31441 aggttcagca cgccgaggct gatgctgacc agtgccaaaa attccaaata actttgcaag
    31501 ccgagttcgg cggactgtcc ggcaatgtcg gcaatggtca gcggcccgga aatatggctg
    31561 acggaggcgt tgccgctgat tagtttgccg aaaaatttga gggttgtcca cgagtgggaa
    31621 acggtttttt cccagcccat gccgaatgcg cggataacag acggacggta gctgcggcgg
    31681 atttgcgcgt cccacgccct gtccggctgc ggacggaggc cgacgcgccc gatcagggtg
    31741 tggtcggact gttcgacagt atcggggcgg atgtcggcgg tatgggtttg tccggcgcgt
    31801 tcgtaggtta aggcgatttt cctgccgggg ctttggcggg tcaggtttgc ccattcctgc
    31861 cacgaggcga tgggtttgcc gtcggcggca gtcagcctgt cgtccggttg cagacctgct
    31921 ttgtcggcgg gactgccttt ttccacgccg ccgacaacgg ttgtgatttt aaagggcatc
    31981 agtccgatgt agccttggtt ttttgcgatt ttaccggctt ccggcgtgcc tgcggcatcg
    32041 atggtgcgga cggtttgcgc gcccgatgcc gtctgaacgc cgacggcgac tttgcctgcc
    32101 tcaaggttga ggacgatttc ggtttgcgcg ctgccccaat ctgcaacggg tgtgccgttg
    32161 acggattgta ttttgtcgcc gctttggaag ccggcgcggg cggcaatggt gtcaggttcg
    32221 actgtgccga cgtaggggcg cagttcggtt acgccgaagg aaaagctcag tccgtacagc
    32281 aaaaccgcca gtgcgaggtt ggtcagtggg ccggcggcga cgatggcgat gcgcttggcg
    32341 gggtgttgtt tgtcaaaagc gtagggtaaa tcggcttctg atacttcgcc ttcgcgcgtg
    32401 tcgaccattt tgacgtaacc gcccaacgga atcggggcga ggcaccattc ggtgtcgccg
    32461 cgctttcggg tgaaaaacgg tttgccgaag ccgacggaaa agcgtacgac tttgacgccg
    32521 cacaatctgg caacgatgta gtgtccgaac tcgtgcaggc tgaccaaaat caggatggcg
    32581 aagataaaag ctagaagggt gtgcaaatgg ttttcctttg ataacggtgt tcggacggca
    32641 tcagcgcagt gtgccgataa atgcccgcgc ttgtgcgcgt gtccgggcat cttgcgccaa
    32701 gagcccccct atgtcgccta tgccgtctga aaagtcttgt gaaagacaat gggcgacggt
    32761 tttggcaatg tcggtaaact taatctgtcc gtccaaaaag gcggcgacgg cggcttcgtt
    32821 ggcggcgttc aatacgcagg gcgcggctcc gcctgcgttc atggcttcat aggcgagctt
    32881 caggcagggg aagcggtcaa agtcgggctt ttggaaggtc agcgcggaca atgcgtcgaa
    32941 atcaaggtcg ccgacacccg aatcgatgcg ctcgggcaag cccaaacaat aggcgatggg
    33001 cgttcgcata tcgggattgc ccagttgtgc caacacggag ccgtcgcggt agcgcaccat
    33061 gctgtgtatc acggattgcg gatggatgac gacttcgagc ttgtcaggcg gacagttgaa
    33121 cagccaatgc gcttcaatca gctccaaacc tttgttcatc atggtggcgg aatcgacgga
    33181 gattttgcgc cccatgctcc aattggggtg tttgaccgct tgggcgggcg taatgctgtc
    33241 gaacgtgcct aaatcggtcg ttaaaaatgg gccgccggaa gcggtcagga taatcgaagc
    33301 gatgccgtgt tcgttcagac gacctgtgta atcgcgcggc aaaacttgga aaacggcgtt
    33361 gtgttcgctg tcgacgggta acactgccgc gctgtttgca cgggcggttt ccataaacaa
    33421 cgcgccggaa accaccagcg tctctttgtt cgccagataa atggttttgc ctttttgcgc
    33481 cgctgcgagc gcggaaggca gccccaccgc cccgacgatg gcgcacatga caccgctgac
    33541 ttcgtcggca gaggcaacgt caaccaatgc ctgcgcgccg tgtaaaacct gagtcgccgt
    33601 gccgtcgcgt ttcaacaggg cttcaagccg ggcggcgtat tcggcatcgg caacgacggc
    33661 atattcgggt tggaacgttt gacattgagc cgccagtttc tcgacctgct tatgccctgc
    33721 cagcgcgaat acgcggaatt tttcggggtg gcgggagaca acgtccagcg tgctttcacc
    33781 tatgctgccg gtactgccta atatggtcag gacttgtggt gtcataatga ggataacttt
    33841 ataccggatg ccgtctgaag cgttttcaga cggcatagaa tcaatttaaa accgacatca
    33901 tcgctgcata gacgctgata acggcaatca ggctgtcggt acggtcgaac acgccgccgt
    33961 gtccgggcag cagcttgctg ctgtctttga tgcctgccgc gcgcttgagc cagctttcca
    34021 aaaggtcgcc gcatacgctg acaacggtca gcaccaaacc gattaacacg gtatcgaacc
    34081 agcctgtatc gaatgccagc cagccggcac ttcgtacggc ggtcatgtac actgccacgc
    34141 aaaccgcgcc gccgattgca ccttcccagc ttttgccggg gctgattgcc ggcgcgattt
    34201 tgtgtttgcc gaacgccttg ccgctgaaat acgcgcaaat atcggcaacc cacaccaaac
    34261 ccatcacggc gagcagcggc agggcatcat cgggatgcgg gcgcagggat acgagcgcga
    34321 accaaaacgg catgaccaga agccagccga cggcataaac ctgccaaccg ccgttgagcc
    34381 tccatttgaa tctcaaccat aaaggcataa cggcgagcca aaatgccaaa acaacatacc
    34441 aaaccaaatt aggcagcatc cagccgcccg cataggcaac cacgccgaaa accaaggttg
    34501 cggcgaggta atggttggtt ttaattttgc acaaaccgcc catacgggca tattcccaca
    34561 aggcaatcag ggcaatcagt ccgcaaaatg cagcccacaa ccattgcggc gcgtaaaaca
    34621 gcatgcccag catcagcggc agcagccaca tggcggttat tacccgttgt ttcagcatat
    34681 tcagttcctt tgctgttcga taggcagttg ctcggaggtg cgtccgaacc gccgttcgcg
    34741 tttttggaac gaagcgacgg catcgtccaa agccttgccg tcaaaatcgg gccacaaaat
    34801 atcggtgaaa tacagttctg catatgccat ctgccagagc aggaaattgc tgatgcgcgt
    34861 ttcgccgccg gtgcggatga acaaatccgg ttccggtgca tcgcccagca tcaagtgttt
    34921 cgccagcgtg tcttccgtaa tctcggatac gccttcggca atcagtttgt ttgccgcctg
    34981 caaaatatcc cagcggccgc cgtaatcggc ggcaatgctc agggtcaggc cggtattgtt
    35041 tgccgtcaac gcttccgcct cttcgatgcc ttgcagaatc tgccggttga agcgttcgcg
    35101 gctgcccaat atcttcaggc gcatattgtt ttcgtgcagg cggcgtacct gtttttgcaa
    35161 agcctgtaaa aacagcccca tcaggaacga aacttcgtct tcggggcggc gccagttttc
    35221 ggttgaaaag gcaaacacgg tcagatattg cacacccagt ttggcgcaat gcttcaccat
    35281 attttccaat gcgtccaaac cgcgtttgtg tcccattatg cgcgggagga aacgtttttt
    35341 cgcccaacgg ccgttgccgt ccataatcac ggcgatatgc ttgggaatgg cggtgtgttc
    35401 caaaacggcc tgcgtgctgc ttttcatgtc tgcctttcgc ggttcggcat tcaaatgccg
    35461 tctgaacgcc gaaccgtgca ggttaaattg ccatcaaatc ttcttctttg gcagtcagga
    35521 gtttgtcggc ttcggtaatg tatttgtcgg tcagtttttg aaccgcttct tcgccgcgac
    35581 gtgcctcgtc ttcggaaatt tctttgtctt tgaggagttt tttgatgtgg tcgttggcat
    35641 cgcggcgcac gttgcggata gagacgcggc cttcttccgc ttcgccgcgt acgactttaa
    35701 tcaggtcttt gcggcgttcc tcggtcagca tgggcatcgg cacgcggatc aggtcgccga
    35761 cagctgccgg gttcagtccc aagtttgaat cgcggatggc tttctcgact ttggccgcca
    35821 tattgccctc aaacggtttc acgccgatgg tgcgcgcgtc cagaagcgtt acgttggcaa
    35881 cttggctgac ggggaccatg ctgccccagt attcgacttc cacttggtcg agcaggccgg
    35941 tatgcgcgcg gccggtacgc actttcgcca gattttcttt cagtacttcg accgaacgct
    36001 gcatcttgcc ttcggctgtt ttttgaatat cgttgatcat attgttcttt cggtgggata
    36061 aggtgggcgg gagaccgtct gaacgcgttt caagccgttc agacggcata aagaccgtta
    36121 accgcgaata gtaccgttat tcgggcataa cgacaaggta ggcggattgg ggatgccgtc
    36181 tgaagcgaca ggcgtttcag acggcatcgt gtccgaccgt cagccgtgtt cccgtgtttc
    36241 aagcaggctt tggcgcaggt gttggcgttc gtgggcatcc agccatttgc ggcgggtgcg
    36301 ttgcagcagg atgacgaggg cggaaatttc ctgacgcata ttggtgctga gccagaggaa
    36361 gccctgccat tggtagtgga ggtgttcggc gagggcttcc agttcggggt tgatggcggt
    36421 gtcgatgcgg atgcggcggg cgtgtctgcc gttgataagg gcgacggttt gttgcaggtc
    36481 ggtttggagc agtgtgaagt ggcggtcaag cagccggatt tcgctgccgt tgagtttggg
    36541 agattgcagc ttggcggcgg tggtcaggag cagctcggtg gtgttgacga ttttacggtg
    36601 ggcgtgctgc atggcttcca tcatggcggg gctgatgcgg ctttcgcccg atgtggcggc
    36661 gagatggctg cggcttttga ccatgcgtgc gttgatttgg cgcattttcg ccatgttctc
    36721 ctcgaggcgt tcgcgggtca tgcgcctgcc gttgctgatt tcggcaatca ttttgctgca
    36781 gtcggccagg ttgtcggcaa gcatgaaacg ccacatcagt gtggatttca gcggcagcag
    36841 tttggcggcg gcgatggcga tggccgcgcc gatgaggacg ttcatggcgc gcatgagtcc
    36901 gctgtcgagc cattcgctgc cgttgtcgcc gatgagcata cacatcgtca gccctgccag
    36961 catagggacg tagccgtttt tgccgaccgc cgcccagccg gccagtgcgc ttgccgtgcc
    37021 gacggtgagg tagaagagga ggttgccgtg gaaataatgc tggttcagcc ataaaacgcc
    37081 caaacccgcg cccagcccga tgaccgtgcc gagcatacgt tccaccgcct tggagtaaat
    37141 cgccccttga aactggagca tgccgaggac gacgaagacg gtcatcccta tccactcgcc
    37201 gtgttggagg tggagcagcc gggcggaggc ggtggcgaac aggacggccc cgccgagccg
    37261 gacggaatgg atgaggcggc ggtagcggta gcgttcgtag gagttgagcc agcggctgac
    37321 gaggcggttg cgttgcgagg tgttcatatc ggttgtgccg tctgaagcgg aaatgtgaaa
    37381 aagcacaggc ttcccgagga agggagggtc tgtgcttggt attggtgccg gagaagggaa
    37441 tcgaaccccc gaccttcgcg ttacgaatgc gctgctctac cgactgagct acaccggcgt
    37501 tttttcgtca tgatatatat gaacggttgt ttgtgcaact tttcgggcgg gcggcaaggc
    37561 agtgcgcg
//
LOCUS       DAXHNE010000004        34067 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.004, whole genome shotgun sequence.
ACCESSION   DAXHNE010000004 DAXHNE010000000
VERSION     DAXHNE010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 34067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34067
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.004"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            142..1116
                     /locus_tag="ACFM4Z_000141"
     CDS             142..1116
                     /locus_tag="ACFM4Z_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6020254.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSL
                     LLPAAPLPVKMSVAAVAALIGMLVFMMLIRRLPPTAQLMVPLVGIIFGGVVEAVATFI
                     AYENEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVN
                     LGLNRTAVLWSGLIIVALITSLVIVTVGNIPFVGLVVPNIISRLIGDRLRQSLPAVAL
                     LGASLVLLCDILGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            1106..2080
                     /locus_tag="ACFM4Z_000142"
     CDS             1106..2080
                     /locus_tag="ACFM4Z_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFC6020255.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCILFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTLGGV
                     GYASLPLTGKFGFELIVMMGGSLLLFYTLIKQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHNELLGIGALILLISAAAVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            2125..2751
                     /locus_tag="ACFM4Z_000143"
     CDS             2125..2751
                     /locus_tag="ACFM4Z_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HFC6020256.1"
                     /translation="MTQERLPDFFDRAPTLTVQDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAFMQGERDEGTTGVTASVVQLLTS
                     AAPETGFGGVGPAGRFARRNLLSFGAGEINGTLTLRRKDNGKTVAVSLNAALQPFAPQ
                     MRDIMPKAVSGSASAEELEHFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            2834..3592
                     /locus_tag="ACFM4Z_000144"
     CDS             2834..3592
                     /locus_tag="ACFM4Z_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6020257.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLADTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTREHKRTTVAVLHDINQAAAYADHVVAMKNGKIALTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
     gene            complement(3651..3989)
                     /locus_tag="ACFM4Z_000145"
     CDS             complement(3651..3989)
                     /locus_tag="ACFM4Z_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFC6020258.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            4084..4296
                     /locus_tag="ACFM4Z_000146"
     CDS             4084..4296
                     /locus_tag="ACFM4Z_000146"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020259.1"
                     /translation="MPETVIPAQVGIPISAFQQYLKVSVNPNFHIPNRAETTAFDLKT
                     YGSSCPPLYFLQLSAYPALGKNQKNR"
     gene            4453..8415
                     /gene="purL"
                     /locus_tag="ACFM4Z_000147"
     CDS             4453..8415
                     /gene="purL"
                     /locus_tag="ACFM4Z_000147"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFC6020260.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKRQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPGLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGGKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFELREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPIDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHADDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPAVALFDAPASGRMCVGEAITNIVAANIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVEDVRKTVTPELKNVEDSVLLFIDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTSRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLA
                     QTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADIINLFYQ
                     QQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRDNPACAD
                     SEFALIGDNERSALFADVKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFT
                     RAGFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQ
                     FAAFFADPDTLTLGVCNGCQMVSNLAEIIPGTAGWPKFKRNLSEQFEARLSMVHVPKS
                     ASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNP
                     NGSPQGIAGITNADGRVTIMMPHPERVYRAAQMSWKPEGWTELSGWYRLFAGARKALG
                     "
     gene            8510..9268
                     /gene="gloB"
                     /locus_tag="ACFM4Z_000148"
     CDS             8510..9268
                     /gene="gloB"
                     /locus_tag="ACFM4Z_000148"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFC6020261.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLDFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            9575..13870
                     /gene="mspA"
                     /locus_tag="ACFM4Z_000149"
                     /gene_synonym="ausI"
     CDS             9575..13870
                     /gene="mspA"
                     /locus_tag="ACFM4Z_000149"
                     /gene_synonym="ausI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/serine protease MspA"
                     /protein_id="HFC6020262.1"
                     /translation="MRFTHTIPFYSVLSTLGLFAVSPAYSSIVRSDVDYQYFRDFAEN
                     KGAFAVGASNISIQDKQGKILGRVLSGIPMPDFRVSNRQTAIATLVHPQYVNSVKHNV
                     GYGSIQFGNDTQNPEEQAYTYRLVSRNPHPDYDYHLPRLNKLVTEISPAALSSVPLLG
                     NGQPKANAYLDTDRFPYFVRLGSGTQQVRKADGTRTQTAPAYQYLTGGTPLKVLGFQN
                     HGLLVGDSLTDQPLNTYAVAGDSGSPLFAFDKHENRWVLAGVLSTYAGFDSFFNKYIV
                     TQPEFIRSTIRQYETRLDVGLTTNELIWRDNGNGDSTLQGLNERITLPIANPSLAPQN
                     DSRHMPSEDAGKTLILSSRFDNKTLMLADNINQGAGALQFDSNFTVVGKNHTWQGAGV
                     IVADGKRVFWQVSNPKGDRLSKLGAGTLIANGQGINQGDISIGEGTVVLAQKAASDGS
                     KQAFNQVGITSGRGTAVLADSQQIKPENLYFGFRGGRLDLNGNNLAFTHIRHADGGAQ
                     IVNHNPDQAATLTLTGNPVLSPEHVEWVQWGNRPQGNAAVYEYINPHRNRRTDYFILK
                     PGGNPREFFPLNMKNSTSWQFIGNNRQQAAEQVAQAENARPDLITFGGYLGENAQTGK
                     AAPSYSKTNEAAIEKTRHIANAAVYGRPEYRYNGALNLHYRPKRTDSTLLLNGGMNLN
                     GEVLIEGGNMIVSGRPVPHAYDHQAKREPVLENEWTDGSFKAARFTLRNHARLTAGRN
                     TAHLDGDITAYDLSGIDLGFTQGKTPECYRSYHSGSTHCTPNAVLKAENYRALPATQV
                     RGDITLNDRSELRLGKAHLYGSIRAGKDTAVRMEADSNWTLSQSSHTGALTLDGAQIT
                     LNPDFANNTHNNRFNTLTVNGTLDGFGTFRFLTGIVRKQNAPPLKLEGDSRGAFQIHV
                     KNTGQEPQTTESLALVSLNPKHSHQARFTLQNGYADLGAYRYILRKNNNGYSLYNPLK
                     EAELQIEATRAEHERNQQAYNQLQATDISRQVQHDSDATRQALQAWQNSQTELARIDS
                     QVQYLSAQLKQTDPLTGILTRAQNLCAAQGYSADICRQVAKAADTNDLTLFETELDTY
                     IERVEMAESELDKARQGGDAQAVETARHAYLNALNRLSRQIHSLKTGVAGIRMPNLAE
                     LISRSANTAVSEQAAYNTGRQQAGRRIDRHLTDPQQQNIWLETGTQQTDYHSGTHRPY
                     QQTTNYAHIGIQTGITDRLSVGTILTDERTNNRFDEGVSARNRSNGAHLFVKGENGAL
                     FAAADLGYSNSRTRFTDYDGAAVRRHAWDAGINTGIKIDTGINLRPYAGIRINRSNGN
                     RYVLDGAEINSPAQIQTTWHAGIRLDKTVELGQAKLTPAFSSDYYHTRQNSGSALSVN
                     DRTLLQQAAHGTLHTLQIDAGYKGWNAKLHAAYGKDSNTARHKQAGIKIGYNW"
     gene            14102..15556
                     /gene="mgtE"
                     /locus_tag="ACFM4Z_000150"
     CDS             14102..15556
                     /gene="mgtE"
                     /locus_tag="ACFM4Z_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFC6020263.1"
                     /translation="MSIEPTPPNLENDGIENDVERVSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLSELHPADVAAVLESLPPRERNIVWILVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTGMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(15642..16550)
                     /gene="hslO"
                     /locus_tag="ACFM4Z_000151"
     CDS             complement(15642..16550)
                     /gene="hslO"
                     /locus_tag="ACFM4Z_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFC6020264.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGQGRLKMLVAEATSDRTVRATARWDEA
                     AEIGEDESLVDLLGEGGVFVLTLQPKDGEPWQGVVPLEGDGIAQMLMNYMKRSEQLDT
                     HIVLSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAGELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVAEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            16795..17550
                     /locus_tag="ACFM4Z_000152"
     CDS             16795..17550
                     /locus_tag="ACFM4Z_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC6020265.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            17569..17787
                     /locus_tag="ACFM4Z_000153"
     CDS             17569..17787
                     /locus_tag="ACFM4Z_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020266.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            17932..18279
                     /locus_tag="ACFM4Z_000154"
     CDS             17932..18279
                     /locus_tag="ACFM4Z_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFC6020267.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRGIIR"
     gene            18276..18968
                     /locus_tag="ACFM4Z_000155"
     CDS             18276..18968
                     /locus_tag="ACFM4Z_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFC6020268.1"
                     /translation="MNEILRQPSVLLFLTLAVYAIAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILGIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            19035..20255
                     /gene="argJ"
                     /locus_tag="ACFM4Z_000156"
     CDS             19035..20255
                     /gene="argJ"
                     /locus_tag="ACFM4Z_000156"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFC6020269.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            20315..21731
                     /locus_tag="ACFM4Z_000157"
                     /pseudo
     CDS             20315..21731
                     /locus_tag="ACFM4Z_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            21921..23312
                     /gene="hrpA"
                     /locus_tag="ACFM4Z_000158"
     CDS             21921..23312
                     /gene="hrpA"
                     /locus_tag="ACFM4Z_000158"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFC6020270.1"
                     /translation="MFPDFSQTLSKDRHFLRSAFKNPNKYGGLSKIEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLEEIKKAIAKNQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARHGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMAALKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAV"
     gene            23456..25099
                     /locus_tag="ACFM4Z_000159"
     CDS             23456..25099
                     /locus_tag="ACFM4Z_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6020271.1"
                     /translation="MDEIQIPKKVELQTKLENEKIVLSKGSTTIIVGANGTGKTRLAV
                     YIEEQLKEKAHRISAHRALKLNPNVNKIPEKSAKTYLSYGQNWDGIDVSNRKNYRWDN
                     NSYTHLLNDFDWLLQYLFAQQNNIAVANNQKLNRNEKVTNSKTKLDILQEAWETLLPH
                     RKLHITADDIQVSAVDNEELYSASNMSDGERALFYILGQVLSVDDGSVLIFDEPELHI
                     HKSIISNLWDKIEELRPDCSFLIITHDIEFAATRVAKKYVIRNYYPTPAWDISEVPES
                     NFDEETITMILGSRKPILFVEGNNNSLDIATYRYCYPDWTIIPKGACKDVIQSVSSLK
                     KLSNEMPLLNLKCSGIVDLDSRDEREIEQLNNLGIYILPVSEIENLFSLTDVAKEILK
                     LNQYSDEELLNKLNGFKSELIKYIDNELKDDKLDEFVVKQVRRKIDNYLKNIDLSSKI
                     TSTDMKKSLLNEISTLTEQKIETWISEIKNEIQRCIEQQDLDKLLTIYDNKGLLAKSA
                     CVLKGMRNKHEFESWIMRTLKGRNKDFIDAIRQKLPILD"
     gene            25143..25325
                     /locus_tag="ACFM4Z_000160"
     CDS             25143..25325
                     /locus_tag="ACFM4Z_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020272.1"
                     /translation="MKNHQTTLSFPTSSSLKPCADKQGLSEDRSQYRLTKLGEQMAHL
                     PIDPKIARILLVLFRF"
     gene            25537..27114
                     /gene="lpt3"
                     /locus_tag="ACFM4Z_000161"
     CDS             25537..27114
                     /gene="lpt3"
                     /locus_tag="ACFM4Z_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HFC6020273.1"
                     /translation="MMKKSFLTLVLYSSLLTASEIAYRFVFGIETLPAAKIAETFALT
                     FVIAALYLFARYKVTRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPVLWGVLEVMLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSRIPAFKQPAPSKIGQGSVQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNAIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENEMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGKHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYLVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRDGKAEYVYPQ"
     gene            27216..28394
                     /locus_tag="ACFM4Z_000162"
                     /pseudo
     CDS             27216..28394
                     /locus_tag="ACFM4Z_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240826.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
     gene            28395..30599
                     /locus_tag="ACFM4Z_000163"
     CDS             28395..30599
                     /locus_tag="ACFM4Z_000163"
                     /inference="COORDINATES: protein motif:HMM:NF023326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HFC6020274.1"
                     /translation="MAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGS
                     RQNENHGNTVGWVENPTSAATAKTVGFDNPTYAAQQTTPSPVGEGRGEGKTVAAQTNF
                     SATAANPLPNPLPQEREQSAAASTISDDLRPANLQQTAPSPVGEGWGEGKTVATQTNF
                     SATSTNPLPQEREQSASASTFSDDLRPANLQQPSPSPVGEGWGEGKTVATQTNFSATS
                     TLSDDSKPKKQPAPQKNRLKPLPLADIRTFQAWLKTAERDNLRLLFLSRDDLMQHAAA
                     HITEEQFPKFWQTADGKFKLSYRFEPHHPLDGVTMTVPLTVLNRLHAPSLEWLVPGMI
                     REKIQLQIKALPKQIRRICVPVPEFITQFLSQNPDRNAPILPQLAQAIAKTAGDIRIF
                     EQINQDEWAAFRLPEHCYFNLRIIDDGGQELAGGRKLHELQQQLGQAAAVTFRDNTQE
                     FERDNVTAWDIGTLPESIKFARGKQQLTGYLGLQKEKDGRIALRLFDTTEAAEQAHRQ
                     GVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGEDEL
                     PRNEKAFKEQIKRARSRLPAVKEALSRYLQETAAVYAELNSKLGKHPLTHLLRLRLQT
                     LLAAGFATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEKTD
                     SLIKQGLPISDGLAAFKWMIEELRVSLFAQELKTPYPVSVKRLLKEWEKIEK"
     gene            complement(30632..31723)
                     /locus_tag="ACFM4Z_000164"
     CDS             complement(30632..31723)
                     /locus_tag="ACFM4Z_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFC6020275.1"
                     /translation="MQTFNRERLEFALDKRGCTQTKLAELLGITARQVRNYVKDEQIP
                     DLNELANLLEFPVHFFTNDERLPELKSQAVSFRARSRTSKRLEKQALNHSITAFLLND
                     WLETEFTLSQAVLPDFSDISPEEAAQELRLEWGLGNEPIPNMITLLEAKGVRVFSLSL
                     DTKDIDAFCTWYENHPFVFLNTQKSAERSRFDAAHELGHLIRDKYSMEHSKNSEITED
                     EPRDIIEKEANAFASAFLMPEAALRLYRHVPITIENLLKIKRRFGVSLVALAYRMHKL
                     GMITDRMYIHNLCPLFAKKKYRTIEPEPMEREISSALRKMLTILKNDGIGIEQIAENL
                     NVSPKDVADLTFGLVERDLNRYRKLRLVK"
     gene            complement(31739..32383)
                     /locus_tag="ACFM4Z_000165"
     CDS             complement(31739..32383)
                     /locus_tag="ACFM4Z_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020276.1"
                     /translation="MVEFALSEPFPFEVGEIVEQQNSDIALSEISKYLSQEMLKEIVL
                     TAHNNIQDKAALSDRNTLGMYRQAEITFALRVYLSKDGWTLEPWSGGAFLCLSPCRTK
                     SIIVATATSDVGRHHGMPMTTARKGKGFEEAIRNSKLDLPDTVQFWVLLFHWKYNKVQ
                     LELSLPSGFVSHQITGYKQRIILADVDLTKFDELERKQPVEEYEPLVERKQKAG"
     gene            32740..32892
                     /locus_tag="ACFM4Z_000166"
     CDS             32740..32892
                     /locus_tag="ACFM4Z_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020277.1"
                     /translation="MSGIYLPRLFPPHIAERGLLYFQQGKVLDVRKTSAGHYRAEVCG
                     SENYWV"
     gene            33149..33417
                     /locus_tag="ACFM4Z_000167"
                     /pseudo
     CDS             33149..33417
                     /locus_tag="ACFM4Z_000167"
                     /inference="COORDINATES: protein motif:HMM:NF017953.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
     gene            complement(33507..33956)
                     /locus_tag="ACFM4Z_000168"
     CDS             complement(33507..33956)
                     /locus_tag="ACFM4Z_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC6020278.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT         8698 a         9889 c         8085 g         7395 t
ORIGIN      
        1 gcgggaatgg cggcaagcgt ttttattgga acttgcccta tcctctgccc gatacggagg
       61 cttcaaacaa ggctttccgc tccgacggtt cggactgcct tgtttgaatc ttctacgcct
      121 taacgctttt cccttctgtt tatgactgcc aaaccttttt ccctcaacct gaccaacctc
      181 ctgctgctgg cggtgttgtt tgccgtcagc ctgtcggtcg gcgttgccga tttccgctgg
      241 tcggatgtgt tttcgctgtc ggacagccag caggttatgt tcatcagccg cctgccgcgc
      301 acgtttgcga ttgtgctgac gggcgcgtcg atggcggtgg cggtggcggg gatgattatg
      361 cagattctga tgcgtaaccg ttttgtcgaa ccttctatgg cgggcgcggg tcagagtgcg
      421 gctttgggtt tgcttctgat gtccctgctg ctgcctgccg cgccgctgcc ggtcaaaatg
      481 tcggttgccg ccgttgccgc gttaatcggg atgctggtgt ttatgatgct tatccgccgt
      541 ctgccgccga ccgcgcaact gatggtgccg ctggtcggga ttattttcgg cggcgtggtt
      601 gaggcggtgg cgacctttat cgcgtatgaa aacgaaatgc tgcaaatgct cggcgtgtgg
      661 cagcagggcg atttttcgag cgtgctgctg gggcggtacg agctgctttg gattacgggc
      721 ggtttggcgg tgtttgccta tctgattgcc gaccggctga cgattttggg gctgggcgag
      781 acggtcagcg tgaatttggg tttgaaccgg acggcggtgt tgtggtcggg gctgattatt
      841 gtggctttga ttacgtcgct ggttatcgtt acggtcggca atattccgtt tgtcgggctg
      901 gtcgtgccga acatcatcag ccgcctgata ggcgacaggc tgcgccaaag cctgcctgcg
      961 gtggccttgt tgggcgcgtc tttggtgttg ctgtgcgata ttttgggacg cgtgattgtg
     1021 tttccgtttg aaattccggt ctctacggtt tttggtgtgt tgggtacggc tttgtttttg
     1081 tggcttttgt taaggaaacc tgctcatgcc gtctgaaaaa aatatcggtt ttatggcagg
     1141 aagcagccgt ccgttgtggg ttgcctttgc gctgttgctg gtttcctgca tcctgtttat
     1201 gacgctcaac gtcaaaggcg attgggactt tgtactgcac ctgcgtctga ccaaacttgc
     1261 cgcgctgctg atggtcgcct atgcggtcgg cgtgtccacg caactcttcc aaacgctgac
     1321 caacaatccg attctgaccc cttcaatttt gggtttcgat tcgctgtatg tgtttttgca
     1381 gaccttgctg gtgtttacgc tcggcggcgt gggctatgct tccctgccgt tgacgggcaa
     1441 attcggcttt gaactgatcg tcatgatggg cggctcgctg ctattgttct acacgctcat
     1501 caaacagggc ggacgcgatt tgccgcgtat gattttaatc ggcgtgattt tcgggatttt
     1561 gttccgcagc ctgtcatccc tgctttcgcg catgatagac cccgaagaat ttaccgcggc
     1621 gcaggcgaat atgtttgccg gattcaatac cgtccacaac gaactgctgg ggataggcgc
     1681 gctgattctg ctcatcagcg cggcggcggt ttggcgcgaa cgctatcgct tggacgtaca
     1741 ccttttgggg cgcgaccaag ccgtcaattt gggcatcagc tacacgcgca acaccttatg
     1801 gatactgctt tggattgccg cattggtggc gacggcgacc gccgtggtcg gcccggtaag
     1861 ctttttcggg cttctcgccg cctcgcttgc caaccacttt tccccgtccg tgcgccattc
     1921 cgtccgcctg ccgatgacgg tttgtgtcgg cggtatcctc ctggtcggcg gacaaaccgt
     1981 attcgaacac ttcttgggca tgaaggcggt attaagcgtg gtggtcgaat ttgcgggcgg
     2041 actcgttttc ctctatctcg ttttaaaaca caaaaaatga cggatgccgt ctgaacggca
     2101 gcccgccccg aaaggacaaa ccatatgacg caagaacgtt tacccgattt tttcgaccgc
     2161 gccccgacgc tgaccgtaca agacccgctt gccgcattcc tcggcgcggc cgaaaacggc
     2221 atcctcactt accgctacgc cgatgccgtg cgcctgtgcg gacattcctg cccgaccgtc
     2281 gcgggcgcgt acctgatggt tatcaaaggt ctgaaagccc tttacggcga agagctgccc
     2341 gaacgcggcg gcatcgaagc ctttatgcag ggtgagcgag acgaaggcac gaccggcgta
     2401 accgcgtccg tcgtccaact cctcaccagc gcagcccccg aaaccggctt tggcggcgta
     2461 ggcccggccg gacgcttcgc ccgccgcaac ctcttatcct ttggtgcagg cgaaatcaac
     2521 ggcacactga ccctgcgccg caaagacaac ggcaaaaccg tcgccgtcag cctcaacgcc
     2581 gccctgcaac ccttcgcacc gcaaatgcgc gacatcatgc ccaaagccgt cagcggcagc
     2641 gcaagcgcag aagaactcga acacttcgga caactctggc aggcacgcgt taaagcattt
     2701 ttgaccgaat cggcggacga cccgcagttc gtcatcgtcc gcgaagtgtg agcgttcaga
     2761 cggcattccg aatttcaaat gccgtctgaa ccccgccaaa caacaacaaa cctacgcccg
     2821 acaagcatcc gccatgatta ccatccgcaa cgtcagctac cgcatcggca cacgccccat
     2881 cctcgacaac gtcagcctcg acatccccga aggcggcatt accgccctcg tcggccccaa
     2941 cggcgcgggc aaatccaccc tgttttcctt tatggcgcgg ctgcgcccgc ttgaaagcgg
     3001 cagcatcgcc taccgcggca aaaaccttgc cgacaccccc accgccgaac tcgcccgcac
     3061 gctctccatc ctcacccaag aaaacagcat catgagccgc attaccgtgc gcgacctgct
     3121 gatgttcggc cgttacccct accatcaagg cagaccgact gccgaatgcc gccgtatcgt
     3181 taacggtgca atcgaagaat tccacctgca agacctctcc gaccgctatc tgaccgagct
     3241 ctccggcgga caacgccaac gcgccatgat tgcgatggtg ttctgccaaa gcaccgacta
     3301 cgtccttttg gacgaaccgc tgaacaacct cgatatgtat cacgcccgct cgctcatgca
     3361 gattctgcaa aagctcacgc gcgaacacaa acgcaccacc gtcgccgtat tgcacgacat
     3421 caaccaggcc gccgcctacg ccgaccacgt cgtcgccatg aaaaacggca aaatcgccct
     3481 gaccggcacg cccgaagaag tgttcaccgt acacaacatc aaagaactgt tcgacatgga
     3541 cgtggacgta ctcgactacg aaggcaaaaa actcatcgtc caccatatct gaagctcaaa
     3601 aagaaaatgc cgtctgaaaa cctttcagac ggcatttcca ttccaaacgc tcaaaccgcc
     3661 gcgtccgcac gttcgcccgt gcggatacgg attgcctcct cgaccggcag cacaaaaatc
     3721 ttgccgtcgc cgatttttcc cgaacgcgcc acctcgacaa tcacgtcaat cgcgcgttcc
     3781 acagcatcat ccgccaacac cagctcgatt ttgaccttgg gcaggaaatc gacggcgtat
     3841 tccgcgccgc gatagatttc cgtatgcccc ttctgtctgc cgaacccttt gacctcgctg
     3901 acggtcatgc ccgtaatgcc gatttccgtc aacgcctcgc gcacgtcgtc gagtttgaac
     3961 ggtttgacaa tcgcctcgat ttttttcatc acattctcca tgaattaacg taaacaaacg
     4021 acaaatatag cagaaaccgc cggcaaacga taaacggtac tgttcgcccg gcgatgcggc
     4081 gatttgccgg aaaccgtcat tcccgcgcag gtgggaatcc cgatctctgc atttcagcaa
     4141 tatttaaagg tttctgtaaa tccaaatttc catattccta accgtgcgga aacgacggca
     4201 tttgatctaa aaacatacgg aagcagttgc ccgcccctct acttcctgca attatccgca
     4261 tacccggctt tgggtaaaaa tcagaaaaac agataaaacc cttattcctt aatatcttca
     4321 gacggcacct gtattttccc caactgtatg ccgtctgaag attgtcaaca ttttaagcca
     4381 aaatacccaa gcaatacagc cccgttgcgc gtataatgac agattttcca accgcatttg
     4441 agagccgaat ccatgtctgt cgttttgccc ttgcgcggcg ttaccgccct ttccgatttc
     4501 cgtgtcgaaa aactcttgca aaaagccgcc gcactcggac tgcccgaagt caaattaagc
     4561 agcgaatttt ggtatttcgc cggcagcgag aaagcacttg atgccgcgac tgtcgaaaaa
     4621 ctgcaagcct tgttggcggc gcaaagcgtt gaacaaacgc caaaagcgcg cgagggcttg
     4681 catttgtttt tggtaacgcc ccgtttgggt acgatttcgc cgtgggcttc caaggcgacc
     4741 aatatcgcgg aaaactgcgg tttggcaggc atcgagcgca tcgagcgcgg catggcggtg
     4801 tggctggaag gtgcgcttac cgatgaacag aaacggcaat gggcggcgtt gctgcacgac
     4861 cgcatgaccg aatcggtgct gcccgatttt cagacggcat ccaaattatt ccaccatctc
     4921 gaatccgaaa ccttttccac cgttgatgtt ttaggcggcg gtaaagaggc tttggtcaaa
     4981 gccaataccg aaatgggttt ggcactctcc gccgacgaaa tcgattatct ggttgaaaac
     5041 tatcaggctt tgcagcgcaa tccgtccgat gtggaactga tgatgttcgc gcaggcaaac
     5101 agcgaacact gccgccacaa aatcttcaac gccgatttca tcctcaacgg cgaaaaacaa
     5161 ccgaaatccc tgttcggcat gatacgcgac acgcacaacg cgcatcccga aggtacggtc
     5221 gtcgcctata aagacaattc gtccgtgatc gaaggcgcga aaatcgagcg tttctatccg
     5281 aatgcggcgg aaaaccaagg ctaccgtttc cacgaggaag acacgcatat catcatgaaa
     5341 gtggaaacgc acaaccaccc gaccgccatc gcgccgtttg caggcgcggc aacgggagcg
     5401 ggcggcgaaa tccgcgacga aggcgcaacg ggcaaaggtt cgcgtccgaa agcaggcttg
     5461 accggcttta ccgtgtccaa cctcaacatc cccggcctca aacagccgtg ggaacaagat
     5521 tacggcaagc cggaacacat ttcctcgccg ctggacatca tgatcgaagg tccgatcggc
     5581 ggcgcggcgt tcaacaacga attcggccgc cccaacctct tgggctactt ccgcactttt
     5641 gaagagaagt ttgacggtca ggttcgcggc taccacaaac cgattatgat tgccggcggc
     5701 ttgggcagca ttcaggcgca gcagacgcat aaagacgaaa tccccgaagg cgcgttgctg
     5761 atccaactgg gcggcccggg catgcttatc ggcttgggcg gcggcgcggc ttcttcgatg
     5821 gataccggca cgaacgacgc ttctttagac ttcaactccg tacaacgcgg caaccccgaa
     5881 atcgaacgcc gcgcgcagga agtcatcgac cgctgctggc agctcggcgg caaaaacccg
     5941 attatctcca tccacgacgt gggcgcagga ggtttgtcca acgccttccc cgaactggtc
     6001 aacgatgccg gacgcggcgc ggtattcgag ctgcgcgaag tgccgttgga agaacacggt
     6061 ttaacgcctt tgcaaatctg gtgcaacgaa tcgcaagagc gttatgtctt gtcgattttg
     6121 gaaaaagatt tggatacctt ccgcgccatc tgcgaacgcg aacgctgccc gtttgccgtg
     6181 gtcggtacag cgaccgacga cggtcatttg aaagtgcgcg acgatttgtt cgccaacaac
     6241 cccatcgatt tgccgttgaa cgtcttgctc ggcaaaccgc ccaaaaccac gcgtaccgac
     6301 aaaacagtta cgccatctaa aaaaccgttt cacgccgacg atatcgacat caccgaagcc
     6361 gcctaccgcg ttttgcgcct gcctgccgta gccgccaaaa acttcctgat taccatcggc
     6421 gaccgcagcg tcggcggcat gacccaccgc gaccaaatgg tcggcaaata ccaaaccccc
     6481 gtagccgact gcgccgttac catgatgggc ttcaatacct atcgcggcga agcaatgtct
     6541 atgggcgaaa aaccggccgt cgccctgttt gacgcgcctg cctcgggcag aatgtgcgtc
     6601 ggcgaagcca tcaccaacat cgtggcagcc aatatcggcg acatcggcaa catcaaactc
     6661 tccgccaact ggatggcggc gtgcggcaac gaaggcgaag acgaaaaact ctaccgcacc
     6721 gtcgaagcgg tttccaaagc ctgtcaggca ttggatttga gcattcccgt aggcaaagac
     6781 agcctgtcga tgaaaaccgt atggcaggac ggcgaagaga aaaaatccgt cgtttcgccg
     6841 ttgagcctga ttatctccgc gtttgcgccg gtggaagacg tacgcaaaac cgttacgccc
     6901 gaactgaaaa acgtcgaaga cagcgtattg ctgtttatcg atttgggctt cggcaaagcg
     6961 cgtatgggcg gctcggcgtt tggtcaggtg tacaacaata tgagcggcga cgcgcccgat
     7021 ttggacgata caagtcgtct gaaagcgttt tacaacgtga ttcagcagct tgtcgccgaa
     7081 gacaaactct tggcgtatca cgaccgcagc gacggcggtt tgtttgccgt tttggtagaa
     7141 atggcgtttg cggggcggtg cggcttggat atagatttaa atttattgct tgcacaaaca
     7201 tttattacca accataccgc tctgtctcaa tcattgcgga ctgaagaggt aaaagcgttg
     7261 gctgaatggc aagaaaccat tgcccgcaca ttatttaatg aagagttggg tgctgttatc
     7321 caagttagaa aacaagatgt tgccgatatt atcaatttat tctatcaaca acagctgcat
     7381 cataatgtct ttgaaatcgg tacgttaact gatgagaaca cgttaatcat ccgcgacggg
     7441 caaacgcacc ttatttctga caacctaatc aaactgcaac aaacctggca agaaaccagc
     7501 catcaaatcc aacgcttgcg cgacaatcct gcctgcgccg acagcgagtt cgcactgatt
     7561 ggcgacaacg aacgcagcgc attgtttgcc gacgtgaagt tcgacgtgaa cgaagacatc
     7621 gccgcgccgt ttatcaacag cggcgcgaaa cccaaaatcg ccatcctgcg cgaacagggc
     7681 gtaaacgggc aaatcgaaat ggccgccgcg ttcacccgtg ccggattcga tgcctacgac
     7741 gtgcatatgt ccgacctgat ggcaggccgc ttccgccttg ccgacttcaa aatgctggcg
     7801 gcgtgcggcg gcttcagcta cggcgacgta ctcggcgcgg gcgaaggctg ggcgaaatcc
     7861 atcctgttcc accctgctct gcgcgaccag tttgccgcct tcttcgccga cccggacacg
     7921 ctgacattgg gcgtgtgcaa cggctgccaa atggtcagca accttgccga aatcatcccc
     7981 ggcacggcag gctggccgaa gttcaaacgc aacctgagcg aacagtttga agcgcgcctg
     8041 agcatggttc atgtccccaa atccgcgtcg ctgattctga acgaaatgca aggctccagc
     8101 ctgcccgtcg tcgtcagcca cggcgaaggc cgcgccgact tcgcgcttca cggcggcaat
     8161 atttccgccg atttgggcat cgcgctgcaa tatgtggacg gtcaaaacca agttacccaa
     8221 acctatccgc tcaaccccaa cggctcgccg caaggcatcg ccggcattac caacgccgac
     8281 ggccgcgtga ccatcatgat gccccacccc gaacgcgtgt accgcgccgc acaaatgagc
     8341 tggaaaccgg aaggctggac ggaactgtcc ggctggtacc gcctctttgc cggcgcacgt
     8401 aaagccttgg gctaaccgcc ctactcaaac caatgccgtc tgaagaatat ttcagacggc
     8461 gttccggcat accatccttt aaacggtatc cgtccaccga ggaacactca tgaaaatcac
     8521 acccgtcaaa gccctaaccg acaactacat ctggatgata cagcacggca accatgccgt
     8581 ctgcgtcgac ccttccgaac cctcgcccgt cttggaattc ctcgtccgca accgcctcat
     8641 gcttgcccaa acatgggtaa ctcaccccca tcccgaccac gagggcggtg cggcggcact
     8701 ctggcgcggc tacatggaat cgcccgttta cggcgaatcc gacatcgaag ccgccaccca
     8761 caccgtaacc gcaggcaccc aattcacctt cggcgacgga caggttaccg tttgggcaac
     8821 acccggccac acagaccgcc acaccagcta ccttctcgaa acttcagacg gcatacacgt
     8881 cttttgcggc gacacccttt tttccgccgg ctgcggacgc gtgtttaccg gcacaatcga
     8941 acagctttac gacagcttcc aacgcttcaa ccgcctgcct gaaaacaccc tgttctatcc
     9001 ggcgcacgaa tacaccgccg ccaacctgga ttttgccgcc catatcgagc cggacaacgc
     9061 cgacattcag acggcactga aagcggcggc gcatacgcct accctgcccg ttaccctcgc
     9121 gcacgaacgc cgtgtcaatc cgtttttgcg cgtcgacctg ccgcacgtca gagaccgcgc
     9181 cgaggcattg agcgggaaaa cgttaaacag cagcctcgat acctttgtcg cgctgcgtga
     9241 acttaaaaac caataccgga cgaaataaaa caacgggaaa acgcagccat tcctaggatt
     9301 tttattaaaa tcttaaataa aatcatacaa tcatcgccaa tagacgaaag gacaccgttg
     9361 ccttataatc aaacaaaaac aaaatatata atatagtgga ttgaatttaa atcaggacaa
     9421 ggcgacgaag ccgcagacaa tacagatagt acggcaaggc gaggcaacgc tgtactggtt
     9481 tttgttaatc cactatataa atccagcaca aaacgggatc ggtgattctt gtccgcaaga
     9541 atcgttgatt ttctctatta cacggataat catcatgcgc ttcacacata ccatcccatt
     9601 ttattccgta ttgtccaccc tcggtctttt tgccgtttcc cctgcttact catccattgt
     9661 ccgcagcgat gtcgattacc aatattttcg cgactttgcc gaaaataaag gcgcgttcgc
     9721 cgtaggtgca agcaatattt ccatccaaga caagcaaggc aaaatattag gcagggttct
     9781 cagcggcatc cccatgcccg acttccgcgt cagcaaccgg caaaccgcta tcgccaccct
     9841 ggttcacccc caatacgtca acagtgtcaa acacaacgtc ggctacggtt ccatacaatt
     9901 cggcaacgac acccaaaacc ctgaagaaca agcctatacc taccgcctcg tatcacgcaa
     9961 cccgcacccg gactacgact accaccttcc ccgcctcaac aaactggtta ccgaaatctc
    10021 acctgccgca ctcagcagcg tacccttgct tggaaacggc cagccaaagg ccaatgccta
    10081 cctcgatacc gaccgcttcc catactttgt acgactcggc tcaggcacgc aacaagtccg
    10141 caaagcagac ggcacgcgca cacaaaccgc cccggcatac caatacctga ccggcggcac
    10201 gccgctgaaa gtattgggtt tccaaaacca cggcttactc gtcggcgaca gcctgaccga
    10261 ccaacccctt aacacctacg cagtcgccgg agacagcggt tcccccctgt tcgccttcga
    10321 caagcatgaa aaccgctggg tgcttgcggg cgtactcagc acctacgccg gcttcgacag
    10381 cttcttcaac aaatacatcg tcacgcaacc cgaattcatc cgttccacca tccgccaata
    10441 cgaaacccgg ctggatgtcg ggctgaccac caacgaactc atatggcgcg acaacggtaa
    10501 tggcgacagc accctgcaag ggctcaacga acgcatcacc ctgcccattg caaacccttc
    10561 gcttgcccca caaaacgaca gcaggcacat gccgtctgaa gatgccggca aaacgctcat
    10621 cctatccagc aggttcgaca acaaaacact gatgctggca gacaatatca accaaggcgc
    10681 aggcgcattg cagttcgaca gcaacttcac cgtcgtcggt aaaaaccaca catggcaagg
    10741 tgcaggcgtt atcgtagccg acggcaaacg cgtcttctgg caagtcagca accccaaagg
    10801 cgaccggctc tccaaactgg gcgcaggcac gcttatcgcc aacggacaag gcatcaacca
    10861 gggcgacatc agcatcgggg aaggcactgt cgtactcgcc caaaaagctg cttcagacgg
    10921 cagcaaacaa gcattcaacc aagtcggcat caccagcggc aggggcacgg ccgtcctcgc
    10981 cgacagccag caaatcaaac ccgaaaacct ctatttcggc ttcaggggcg gacggctcga
    11041 cctcaacggc aacaaccttg cctttaccca tatccgccat gcggacggcg gcgcgcaaat
    11101 cgtcaatcac aaccctgacc aagccgcgac actgacgctg accggcaacc ccgtcctcag
    11161 tcccgagcat gtcgagtggg tgcaatgggg caaccgtccg caaggcaacg cggcggttta
    11221 cgaatacatc aacccgcacc gcaaccgtcg gaccgactac ttcatactca aacccggcgg
    11281 caacccgcgc gaatttttcc cgttaaatat gaaaaactca acaagctggc aatttatcgg
    11341 caacaacagg caacaggccg ccgaacaagt cgcccaagcc gaaaatgccc gccccgacct
    11401 gattaccttc ggcggatact tgggtgaaaa cgcgcaaacg ggcaaagccg cgccgagtta
    11461 cagcaaaacc aatgaagcag ccatagaaaa aacccgccat atcgcaaatg ccgccgtata
    11521 cggccggccc gaataccgtt acaacggcgc actcaacctg cactatcgtc ccaaacgcac
    11581 cgacagcacg ctgttgctca acggcggcat gaaccttaac ggggaagtct tgattgaggg
    11641 cggcaatatg attgtgtcag gcaggcccgt accccatgcc tacgaccacc aggccaaacg
    11701 cgaacccgtt cttgaaaacg aatggaccga cggcagcttc aaggctgcac ggttcaccct
    11761 gcgaaaccat gcccgactga cggcagggcg caataccgcg catctggacg gcgacataac
    11821 cgcatacgat ctgtccggca tcgacctcgg ctttacccaa ggcaaaacac cggaatgcta
    11881 ccgctcctac catagcggca gcacccactg cacacccaac gccgttttaa aagccgaaaa
    11941 ctatcgtgca ctacctgcaa cgcaagtacg cggcgacatt acccttaacg accgttcaga
    12001 gctccgcctg ggcaaagcac acctgtacgg cagcatccgt gccggcaaag acaccgcagt
    12061 ccgcatggaa gcagacagca actggacact ttcccagtcc agccacaccg gcgcactgac
    12121 gcttgacggc gcacaaatta ccctgaaccc cgatttcgcc aataatacac acaacaaccg
    12181 cttcaacaca ctgaccgtca acggcacact tgacgggttc ggcacattcc gattcctgac
    12241 cggcatcgtc cgaaaacaaa atgccccccc cctcaaactg gaaggggaca gccgcggcgc
    12301 attccaaatc cacgtcaaaa acaccggaca agaacctcaa acaaccgaat cgcttgcact
    12361 tgtgagcctc aatccgaaac acagccacca agcccgattc accctccaaa acggctatgc
    12421 cgatttgggt gcctaccgct acatcctccg caaaaacaac aacggataca gcctgtacaa
    12481 cccgctcaaa gaggccgaac ttcaaattga agccacgcgt gcggaacatg agcgcaacca
    12541 acaggcatac aaccaattac aggcaaccga catcagcaga caggttcaac atgactctga
    12601 cgcgaccagg caggcactac aggcctggca gaacagtcaa accgaacttg cccgcatcga
    12661 cagccaagtc caatatctgt ccgcccaatt gaaacagaca gacccgctga ccggcattct
    12721 gacgcgtgcc caaaacctgt gtgccgcaca aggatacagt gccgatatct gccgtcaggt
    12781 tgccaaagcc gccgacacga acgacctgac actcttcgaa accgaactgg atacgtatat
    12841 agaacgtgta gaaatggccg aatccgaact tgacaaagca cggcaaggcg gcgatgcgca
    12901 agccgtcgaa acagcccggc acgcctacct gaacgcactc aaccgtctgt cccgacaaat
    12961 ccacagtttg aaaaccggcg ttgccggcat ccgtatgccg aacctggccg aactgatcag
    13021 ccggtcggcc aacaccgccg tttccgaaca ggccgcctac aataccggcc ggcaacaggc
    13081 gggacgccgc atcgaccgcc accttaccga tccgcagcag caaaacatct ggctggaaac
    13141 cggtacgcaa caaaccgact accatagcgg cacacaccgt ccctaccaac aaactaccaa
    13201 ctatgcacat atcggcatcc aaaccggcat caccgaccgt ctcagtgtcg gtacgatttt
    13261 aaccgatgag cgcacaaaca accgttttga tgaaggcgta tccgcccgaa accgcagcaa
    13321 cggcgcacat ctgttcgtca aaggggaaaa cggcgcactc tttgccgcgg cagatttagg
    13381 ctacagcaac agccgtaccc gatttaccga ttatgacggg gctgccgtcc gccgccacgc
    13441 atgggatgca ggcatcaaca ccggcatcaa aatcgatacc ggcatcaacc tcagacccta
    13501 tgccggcatc cgtataaacc gcagcaacgg caaccggtac gtactcgacg gcgcagagat
    13561 aaacagcccg gcgcaaatcc aaaccacatg gcatgccggc atccgtctcg ataaaaccgt
    13621 cgaactgggt caagccaagc tgacccccgc tttcagcagc gattactacc atacccgcca
    13681 aaacagcggt tccgccctca gcgtcaacga ccgtacctta ctgcagcaag ccgcccacgg
    13741 cacactgcat accctgcaaa tcgacgccgg atacaaaggc tggaacgcca aacttcatgc
    13801 cgcttacggc aaagacagca acaccgcccg ccacaaacag gcaggaatca aaataggcta
    13861 caactggtaa caagccgata aaaatgccgt ctggaacccg cgtttcagac ggcatttgcg
    13921 ttaaaaatag taaaccgttc caaaagggag tagaatagtg ccgtttccaa ccctgcgcct
    13981 gtaccgtcag gcttttatta tggaccttcc cagttcgttt ttactgaaca ccccatccga
    14041 ttcaaacccg caataaccat cccgccggaa tgcctccccg cacacggcgg gcggagcatt
    14101 tatgagcatc gaaccaaccc ctccgaacct tgaaaacgac ggtatcgaaa acgatgtaga
    14161 acgcgtttcc gccgatttcg accgtgtcca ctccctctgc gaaatcctcg aacctgcttt
    14221 tgaacaaatc gaaaacggta caccgctcga agacgcgccg ctgcgcgaca agctgaccga
    14281 gctgaccgtc ctcttgagcg agctgcaccc tgccgacgtg gcggcggtat tggaatcgct
    14341 accgccgcgc gaacgcaata tcgtctggat tctggtcaaa ccggaagacg acggcgaagt
    14401 attgctggaa gtatccgacg cggtgcgcga aacgctgatc gagtcgatgg acaaagacga
    14461 attgttggca gcggtcgatg atttggacgc ggacgaattg gcggaactgg cagacgattt
    14521 gccgcaccaa gtggtttacg aagcgctaca aacccgcgat gaggaagaac gcgcccaagt
    14581 caaagcggca atgtcctacg aagacaacca agtcggtgcg attatggact tcgagttggt
    14641 cagcatccgc gccgatgtcg cctgtgaagt ggtgctgcgc tatctgcgcc gcttcgacag
    14701 cctgcccgac cataccgaca agatttttgt ggtcgatgaa aacgacgtac tgcaaggcgt
    14761 gctgcccatc cgcaaacttt tggtcgccga tcccgaagat ttggtggaaa acgtgatggc
    14821 gaaagatgtc gtgcgtttcc gcgccgaaga cgacgtggaa gaagcggcgc aggcgtttga
    14881 acgctacgac ttggtaactg cgccggttgt cgatgaaaac aaaaagctca tcggtaggat
    14941 taccgttgac gagatggtgg acgtgatccg cgaagaatcc gaagcggata tgttgaatat
    15001 ggcgggtttg caagaagagg aagacttatt cgcccccgtc ttggattccg tcaaaaaccg
    15061 ctggatgtgg ctcgccgtca acctctgcac cgccttcctc gccagccgcg tcatcggcgc
    15121 gtttgaaggc agcatcgaaa aaatcgtcgc actcgccgcg ctgatgccca tcgtcgccgg
    15181 catcggcggt aactcaggca accagacgat taccatgatt gtccgcgcca tggcgatggg
    15241 gcaactgaca ggcatgcagg caggcaggct gttgaaaaaa gaagtcggcg ttgccctagt
    15301 caacggcatt atctggggaa ccgtcatggg cgcggtttca tggctgctct acggcagcct
    15361 cggcatcggg ctggttatga ttgccgcgat gacgctcaac ctcctgctgg cggcaaccgt
    15421 cggcgtatta attcccgtgg taatggaaaa gttcggacgc gatcccgcac tgggcagctc
    15481 ggtgctgatt accgccgtta ccgactccgg cggcttcctg attttcttgg ggctcgccac
    15541 cctattcctg ctttaaatgc cgtctgaacc cgcgcaaaaa tgccgtctga agcggaagct
    15601 gcttcagacg gcatttgact atttatcctt gttgcacaag attattggac ggtatgccgg
    15661 ggcagccctt tggcaacgcc gaccacatcc tccccgaaca gcgcgttgac atcggtttcg
    15721 tcaaacacat atttgctgtg gcagaaatcg caatcgactt cgatgctgcc ttgttccgcc
    15781 accacgccgc cgacttcttc cccgcccagc atcaacagca tatcgctgac tttgccgcgc
    15841 gaacaggtgc atgaaaattc aaacgtttcc ggctcgaaca cgcgcggcgg cgtttcgtgg
    15901 aacaagcggt acaaaacatg ttgcgcatcc aattctgcca actcgcccgc cgtcagcgtg
    15961 cgcgccagcg tactgacgtg ttcccatgcc tcttcatcca acacctcttc aggcagacgc
    16021 tgcaccagca gaccgcccgc cgcttcatcg cttgcagaca ggacgatgtg cgtatcaagc
    16081 tgttcggaac gtttcatata gttcatcaac atttgcgcga taccgtcgcc ttccaaaggc
    16141 actacgccct gccagggttc gccgtctttg ggctgcagcg tcagcacgaa tacgccgccc
    16201 tcgcccaaaa gatcgaccaa actttcatct tcaccgattt ctgccgcctc gtcccaccgc
    16261 gcggttgcac ggacggtacg gtcggaagtc gcttctgcaa ccagcatttt cagccgcccc
    16321 tgcccctgaa cctgcacaat cagcgtgcct tcgtttttaa ggttgcccga cagcagaacg
    16381 ccggcagcca gtagctcacc caaggcgcgg cggatggcgg cgggatagtt tttctgtttt
    16441 acaatgtgct gccacacgtt ttccagacgg acgtgcagcc cgcgcacggg catatcgtcg
    16501 aagataaagc gggtacgcac atcggcgcgg ttgatggcgg tttgattcat gattttctct
    16561 gactgattgt tcggatggcg gctatatggt tgcggtcggc gcgaaaacaa gacggacggc
    16621 ggatgcgctt cccaaattat caataaatta tataaaaatc aacatattaa ctcaatctaa
    16681 caagccgttt tttgccaaac agccgttttt ttatatacaa tcaacaagat attttcgact
    16741 gatacagcat aacatcgcac ggcggcacga tgcctcctgc gcggaaacac cgatatggat
    16801 tcttttttca aaccggcagt ttgggcggtt ttgtggctga tgtttgccgt ccgccccgcc
    16861 cttgccgacg agttgaccaa cctgctcagc agccgcgagc agattctcag acagtttgcc
    16921 gaagacgaac agcccgtttt acccatcaac cgagcccccg cccggcgggc gggcaatgcc
    16981 gacgaactca tcggcagcgc gatggggctt aacgaacagc ccgttttacc cgtcaaccga
    17041 gtccccgccc ggcgggcggg caatgccgac gaactcatcg gcaacgcgat ggggcttaac
    17101 gaacagcccg ttttacccgt caaccgagcc cccgctcggc gggcgggcaa tgccgacgaa
    17161 ctcatcggca acgcgatggg acttttgggt attgcctacc gctacggcgg cacatcggtt
    17221 tctaccggtt ttgactgcag cggcttcatg cagcacatct ttaaacgcgc catgggcatc
    17281 aacctgccgc gcacgtcggc agaacaggcg cggatgggta cgccggttgc ccgaagcgaa
    17341 ttgcagcccg gggatatggt gtttttccgc acgctcggcg gcagccgcat ttcccatgtc
    17401 ggactttata tcggcaacaa ccgcttcatc cacgcgccgc gcacggggaa aaatatcgaa
    17461 atcaccagcc tgagccacaa atattggagc ggcaaatacg cgttcgcccg ccgggtcaag
    17521 aaaaacgacc cgtcccgctt tctgaactga tttcccaagg aatacgcaat gagtatgccc
    17581 gaaatgccca aatggtacga cgatgacgga cagatcgtgt cctgtaccga aaaggtcaaa
    17641 gtgatgtccg aaaatatggc cgagctgtat cagacggcac aagacgcgtt tgaagacgcg
    17701 ctgctgatgg gttgcggcga acgtcagttg cgcgattacc tgctcgcgct gattgaaggt
    17761 ttggaaaatc cctaccgcaa agtctgaaca cgccccggtt gctgcggcac ggtttatccg
    17821 tgccgttttt gcgtttgtgc gcggcttcgg cttttcagac ggcatatttg acgttatgat
    17881 taaacagtta acaagattta tcacaacgcc gtcaaaagac agacacacaa catgaacatc
    17941 atccgcgcgc tcctcatcat cctcggctgc ctcgccgccg gcgaaaccgc cgttttccta
    18001 gcaggcatca aactgcccgg cagcatcgtc ggcatgggcg tgctgtttgc gcttttgcag
    18061 gcgggttggg tcaaaacgtc ttggctgcaa cagcttaccg acgcgctgat ggcgaatctg
    18121 acgctgtttc tcgtgccgcc ctgcgtggcg gttatcagct atttggattt gattgccgac
    18181 gattggtttt cgatactggt ttccgcctcc gccagcactt tgtgcgtact gctggttaca
    18241 ggcaaggttc accggtggat acggggtatt atccgatgaa cgaaatcctc aggcagccca
    18301 gcgttctgct tttcctcacg cttgccgtgt acgcgattgc gattatcgtg cgcacgcgca
    18361 cgggtaatat cttctgcaac cccgtactcg tcagcactat cgtgctgatt gcctacctga
    18421 aaatcctcgg tatcgattat gcggtgtacc acaacgccgc gcaattcatt gatttttggc
    18481 taaaacccgc cgtcgtcgtg cttgccgtgc cgctctacca aaaccgccgt aaaatcttca
    18541 accagtggct gcccgtcatc gtttcacagc ttgcgggcag cgttacgggc attgttacgg
    18601 ggatgtattt tgccaaatgg ctgggcgcgg aacgcgaagt cgtcctctcg ctcgcgtcca
    18661 aatctgttac caaccccatc gccattgaaa tcacccgctc catcggcggc attcccgcca
    18721 ttaccgccgc caccgtcatc attgccggtc tggtcggaca gattgccggt tacaaaatgc
    18781 tgaagaacac ggtcgtcatg ccctcgtccg tgggtatgtc gctcggcact gcctcgcacg
    18841 cgatggggat tgccgcctcg ctcgaacgca gccgccgcat ggcggcatac gcggggctgg
    18901 ggctgacgtt caacggcgtg ctgaccgcgc tgattgcgcc gctgctcatc cccgttttgg
    18961 ggttctgaac ccgtttcaga cgacatttca gcccatgctg tctgaacgcc gacacactcg
    19021 caaggagaac cgttatggct gtcaacctga ccgaaaaaac cgccgaacaa ctgcccgaca
    19081 tcgacggcat tgccctctac accgcccaag caggcgtgaa gaagcccggg cataccgacc
    19141 tgacactgat tgccgtagcc gccggcagca ccgtcggtgc agtcttcacg accaaccgtt
    19201 tctgtgccgc gcccgtccac atcgccaaat cgcacctttt cgacgaagac ggcgtgcgcg
    19261 ccctcgtcat caacacgggc aacgccaacg cgggtacggg cgcacagggc agaatcgatg
    19321 ctttggcagt gtgtgccgcc gccgcccggc aaatcggctg caaaccgaac caggtgctgc
    19381 ccttctccac cggcgtgatt ctcgaaccgc tgcccgcaga caaaatcatc gccgcgctgc
    19441 ccaaaatgca gcctgccttc tggaacgaag cggcacgcgc catcatgacc accgacaccg
    19501 tgcccaaagc cgcctcgcgc gaaggcaagg tcggcgacaa acacaccgtc cgcgccacgg
    19561 gcatcgccaa aggctcgggc atgattcatc ccaatatggc gaccatgctc ggtttcatcg
    19621 ccaccgatgc caaagtttcc caacccgtcc tccaactgat gacacaggaa atcgccgacg
    19681 aaaccttcaa caccatcacc gttgacggcg acaccagcac caacgacagc ttcgtcatca
    19741 tcgccaccgg caaaaacagc caaagcgaaa tcgacaacat cgccgacccg cgttacgccc
    19801 aactcaaaga attgttgtgc agcctcgcgc tcgaactcgc ccaagccatc gtccgcgacg
    19861 gcgaaggtgc gaccaagttc atcaccgtcc gcgtcgaaaa cgccaaaacc cgcgacgaag
    19921 cccgccaagc cgcctacgcc gtggcacgtt cgccgctggt caaaaccgcc tttttcgcct
    19981 ccgaccccaa cctcggcagg ctgctcgccg ccatcggtta tgccggcgtt gccgacctcg
    20041 ataccgacct cgtggaaatg tatctcgacg atattttggt tgccgaacac ggtggacgcg
    20101 ccgcaagcta caccgaagca caagggcagg cggtgatgtc gaaggccgaa atcaccgtcc
    20161 gcatcaagct gcatcgcgga caagccgccg ccaccgtcta tacctgcgac ctgtcgcacg
    20221 gatacgtttc catcaacgcc gactaccgtt cctgacccga cacggcttca gacggcatac
    20281 ataaaatgcc gtctgaaccg ccagacaaca taccatgacc tccacattcc cccgccgcct
    20341 cgcccgcaaa atccgccaaa cccgccgcct gtcgcgcaaa agcatcgcct ttctgttcct
    20401 tttggcaggt tcggcactcg tcgccctgac cgcgctgttt tttgcccatc ttgccgattt
    20461 tgcgctggaa ctgaacgcca aactggttca acaatacccg tggttcgcgt gggtcgcgct
    20521 tcctttgggt ttaccgctta ttgcgtggct cacacgcaaa ttcgccccct tcaccgccgg
    20581 cagcggcatc ccgcaggtca tcgcctcact gtcgctgccc tacggcgcac agaaaacgcg
    20641 gctgatccgc ctcgggcaga cgctgctgaa gattccgcta acctttttgg gtatgctgtt
    20701 cggcgcgtcc atcggacgcg aaggtccgtc cgtgcaggtc ggcgcggcag tgatgggcgc
    20761 gtggggcgcg tggtgcaaga aacacggctt ggcattcaaa gggatgcagg aaaacgattt
    20821 gatggcggcg ggcgcggcgg gcggtttggc agccgcgttc aacgcgccgc tggcgggcgt
    20881 gattttcgcc attgaggaac tcgggcgcgg catcatgttg cgctgggaga ggcaaattct
    20941 tttgggcgtg ctcgcctccg gtttcataca ggtcgccatt cagggcaaca acccgtattt
    21001 ttccggcttc aacggcggcg tattggaaca tatctttctg tgggtcgcac tgtccggcct
    21061 ggtttgcggc gcggcgggcg gcctgttcgg acgtttgctc tatcgcggtg cggcggcgtt
    21121 tgcaccgcgc aagatacgcg gcttcatccg caaccgtccg ctgctgctgg cggcactgat
    21181 ggggctgctg ctcgccctgc tcggcacgtt ctaccaaggc aaaacctacg gcactggcta
    21241 ccacgaagcc gcccaagccc tgcacggcat ctacgaagcc cccttcggac tcgccgccgc
    21301 caaatggctc gccaccgtat tcagctattg ggcaggcatt ccgggcggca ttttcactcc
    21361 ctcgctgacc ataggcgcgg ttttgggcga gcatatcgcc gccatcgccg acatatcgca
    21421 gggtgcaaac atcatcgtcc tcatctgcat ggcggcattt ctggcgggcg cgacacaatc
    21481 cccgattact tccgccgtcg tcgtcatgga aatgacgggc ggacaaagcc tgctgttttg
    21541 gatgctgatt gcctgcattt tcgcctcgca ggtttcgcgc cagttttcgc cgcgtccgtt
    21601 ctaccacgca tcgggaatgc gcttccgcca gcgcgtgctt caagaaaccg ccgcccaaac
    21661 cggcaattcg cccgcaagac cgcaaacagc aaacagcaaa cagcaaaacg ggaatgccgt
    21721 ctgaaaatta aaacgccccc gatcaaacgc cggcagccgc cttgatttga ataccgttcc
    21781 gccgccgctt gaaatttcag caacaatgcc gtctgaacga cagaatgcgg ttttcagacg
    21841 gcatttcccc atcccgatat tgcctaaaca aaaccgaagc gtttgctata attctatttt
    21901 ttaccgcata cgcaccaatc atgtttcccg atttctccca aaccctctcc aaagaccgcc
    21961 acttcctgcg ttccgccttc aaaaatccca acaaatacgg cggtttgtcc aaaatcgaag
    22021 aaaaataccg aaaatcgcac gaaatctttt tgaagcgttt ggcagccttg ccaaaacccg
    22081 aattcgacaa caccctgccc gttcacgaga agctcgaaga aatcaaaaaa gccattgcca
    22141 agaatcaggt aacgattatt tgcggcgaaa ccggttcggg caaaaccacg cagttgccca
    22201 agatttgctt ggaactcggg cgtggggcgg caggattgat cgggcatacc cagccgcgcc
    22261 gtttggccgc gcgctccgta gcagagcgga ttgccgaaga gctgaaatcc gaaatcggca
    22321 gcgcggtcgg ctataaagta cgcttcaccg accacacctc gcgcgatgcc tgcgtcaagc
    22381 tgatgaccga cggcatcctg ctggcggaaa cgcagaccga ccgttatctc gccgcctacg
    22441 acacgattat catcgacgaa gcgcacgagc gcagcctgaa catcgacttc cttttgggct
    22501 atttgaaaca actcctgccg cgccgccccg atttgaaagt catcatcacc tcggcaacga
    22561 tagacgcaga acgcttctcc cgacacttca acggcgcgcc cgttttagaa gtgagcggac
    22621 ggacgtatcc cgtcgaaatc ctctaccgac cgctgaccgg caaagacgaa gacgacgcag
    22681 aagtggagtt gaccgacgcg attgtcgatg cggcggacga attagcgcga cacggcgaag
    22741 gcgatatttt ggtattcctg ccgggcgagc gcgaaatccg cgaagccgcc gaagccctgc
    22801 gcaaatccac cctgcgccgc aacgacgaaa tcctgcccct gttcgcacgc ctgtcgcacg
    22861 ccgaacagca caaaatcttc cacccctcag gcgcgaaacg ccgcatcgta ttggcaacca
    22921 acgtcgccga aacctcgctt accgtgccgg gcatcaaata cgtcatcgac accggcctcg
    22981 cgcgcgtcaa acgctattcc gcacgggcga aagtggagca gcttcatatc gaaaaaatct
    23041 cccaagccgc cgcccgccaa cgatccggcc gctgcggacg cgtctccgca ggcgtgtgta
    23101 tccgactgtt ttcagaagaa gattttaaca gccgccccga atttaccgac cccgaaatcg
    23161 tccgcagcaa cctcgccgcc gtcatcctgc gcatggcagc attgaaactc ggcgatgtgg
    23221 cggcattccc gtttttagaa atgcccgatt cacggtatat caatgacggt tttcaggtgt
    23281 tgttggagtt gggggcggtg gaggccgtct gaaaacaggc agacataaaa gaaaatccgc
    23341 gtagagtgat gtaaacttac ccttgcttta ataagtagaa aatggtgggt ttacgtcccc
    23401 ccctgcggct actaaaaaaa tataagagta aacaaccttt ttgaaagaaa aatgtatgga
    23461 cgaaattcaa atacccaaaa aagtggaatt acaaaccaaa ctagaaaatg aaaagattgt
    23521 tttatcgaaa ggttctacca cgattattgt tggtgctaat ggcacaggga aaacaagatt
    23581 agctgtttat attgaagaac aattaaagga aaaagcacac agaatttcgg ctcatagagc
    23641 attaaaatta aaccctaatg tcaataaaat accagaaaag agtgccaaaa catatctatc
    23701 ttatggtcag aactgggatg gaatcgatgt atcaaataga aaaaattata gatgggataa
    23761 taactcatat actcatttac tcaacgattt tgattggtta ttacaatatt tattcgctca
    23821 acaaaataat attgcggtag caaataatca aaagctcaac cgtaatgaaa aagtaaccaa
    23881 ttcaaaaaca aagctagata ttttgcaaga agcatgggaa acattattac cacacagaaa
    23941 attacatatt acagcagatg atattcaagt ctctgctgta gataatgagg aattgtattc
    24001 tgcctcaaat atgagtgatg gagagcgagc acttttctat attcttggac aagttttgtc
    24061 agtagatgac ggttctgtct taatttttga tgagcctgaa ttacatattc ataaatcaat
    24121 tatttcaaat ctatgggata aaattgaaga attacgacct gattgttcat ttctaatcat
    24181 tacacacgat attgaatttg ctgcaactcg agtagctaaa aaatatgtta tcagaaatta
    24241 ttatccgacc cctgcttggg atatttctga agttcctgaa agtaattttg atgaagaaac
    24301 aataacgatg attttaggta gccgtaagcc aatattattt gttgagggca acaataatag
    24361 tttagatatt gctacttacc gctattgtta tcctgattgg accatcatac ccaaaggggc
    24421 atgcaaagat gtcattcaat cagtatcatc gctgaaaaaa ttaagtaatg aaatgccatt
    24481 actaaactta aaatgttcag gtattgtcga tttagatagt agggatgaaa gagaaattga
    24541 acaattaaat aatttgggta tttacatttt acctgtatcc gaaattgaaa atctttttag
    24601 cttaactgat gtagcaaaag agatattgaa actaaatcaa tattcagatg aagaattact
    24661 caataaactt aatggattta aatccgaact aattaaatat atagataatg aattaaaaga
    24721 cgataaatta gacgaatttg ttgtaaaaca ggttcgacgt aaaattgata attatttaaa
    24781 aaatattgat ttatcctcca aaataacaag tactgatatg aaaaaatcat tacttaatga
    24841 aatttctact ttaacagaac agaaaattga aacatggatt tcagaaatta aaaatgaaat
    24901 tcaaagatgt attgaacagc aagatttgga taaattactt actatatatg ataataaagg
    24961 actcttggct aaatcagctt gtgttttaaa aggaatgcgt aacaaacatg aatttgaaag
    25021 ctggataatg agaacattaa aaggaaggaa taaagatttt attgatgcaa tcagacagaa
    25081 acttccaatt ctggattaaa taaaaccatc tgaaaattta ccttcagata cagatatatt
    25141 tcatgaaaaa tcatcaaact acactctctt tccctacttc gagtagcctg aaaccttgcg
    25201 cagacaaaca aggcctgtct gaagaccgca gccaataccg cctgaccaaa ctcggcgaac
    25261 aaatggcgca cctgcctatc gacccgaaaa ttgcgcgtat tttgttagta ttattccgtt
    25321 tttaaaaatg cccgattcgc ggtatatcaa tgacggtttt caggtattgc tggaattggg
    25381 ggcggtggag gccgtctgaa aataaaatct ttctttataa aaaggcaggc catgtttcat
    25441 tttcagacgg cctaaatcat tgagaaacta aaaactatta aaaagggaat attgggtttt
    25501 aaaactcaat cggtaaattt ttattgtgaa atattaatga tgaaaaaatc tttccttacg
    25561 cttgttctgt attcgtcttt acttaccgcc agcgaaattg cctatcgctt tgtatttggg
    25621 attgaaacct taccggcggc aaaaattgcg gaaacgtttg cgctgacatt tgtgattgct
    25681 gcgctgtatc tgtttgcgcg ttataaggtg acgcgtttgt tgattgcggt gttttttgcg
    25741 ttcagcatta ttgccaacaa tgtgcattac gcggtttatc aaagctggat gacgggcatc
    25801 aattattggc tgatgctgaa agaggttacc gaagtcggca gcgcgggtgc gtcgatgttg
    25861 gataagttgt ggctgcctgt gttgtggggc gtgttggaag tcatgttgtt ttgcagcctt
    25921 gccaagttcc gccgtaagac gcatttttct gccgatatac tgtttgcctt cctaatgctg
    25981 atgattttcg tgcgttcgtt cgacacgaaa caagagcacg gtatttcgcc caaaccgaca
    26041 tacagccgca tcaaagccaa ttatttcagc ttcggttatt ttgtcggacg cgtgttgccg
    26101 tatcagttgt ttgatttaag caggattccc gcctttaagc agcctgctcc aagcaaaatc
    26161 gggcagggca gtgttcaaaa tatcgtcctg attatgggcg aaagcgaaag cgcggcgcat
    26221 ttgaagctgt ttggctacgg acgcgaaact tcgccgtttt taacccggct gtcgcaagcc
    26281 gattttaagc cgattgtgaa acaaagttat tccgcaggct ttatgactgc agtgtccctg
    26341 cccagttttt tcaatgcgat accgcacgcc aacggcttgg aacaaatcag cggcggcgat
    26401 accaatatgt tccgcctcgc caaagagcag ggctatgaaa cgtattttta cagcgcgcag
    26461 gcggaaaacg agatggcgat tttgaactta atcggtaaga aatggataga ccatctgatt
    26521 cagccgacgc aacttggcta cggcaacggc gacaatatgc ccgatgagaa gctgctgccg
    26581 ttgttcgaca aaatcaattt gcagcagggc aagcatttta tcgtgttgca ccaacgcggt
    26641 tcgcacgccc catacggcgc attgttgcag cctcaagata aagtattcgg cgaagccgat
    26701 attgtggata agtacgacaa caccatccac aaaaccgacc aaatgattca aaccgtattc
    26761 gagcagctgc aaaagcagcc tgacggcaac tggctgtttg cctatacctc cgatcatggc
    26821 cagtatgttc gccaagatat ctacaatcaa ggcacggtgc agcccgacag ctatctcgtg
    26881 ccgctagtgt tgtacagccc ggataaggcc gtgcaacagg ctgccaacca ggcttttgcg
    26941 ccttgcgaga ttgccttcca tcagcagctt tcaacgttcc tgattcacac gttgggctac
    27001 gatatgccgg tttcaggttg tcgcgaaggc tcggtaacgg gcaacctgat tacgggtgat
    27061 gcaggcagct tgaacattcg cgacggcaag gcggaatatg tttatccgca atgagtggcg
    27121 taaaaaccaa taaagacaaa tttagatgat gtcggggaag atgcccgacc gacaagacta
    27181 tgcaaaatat gaaaaaccaa gtacgcggat caggcatgga tgcccgatcc aatccggcca
    27241 atgtttcaga cggcctgcaa aacagttcgg gtcatatcgg taccaacacg cgttaccgcc
    27301 tgaccaaact cggcgaacag atagcgcgcc tacccatcga cccgaaaatc gcgcgcattt
    27361 tgctggcggc gaagaaacac gactgcatgg cggaaatatt ggtgattgcg tccgcgctgt
    27421 cgattcaaga cccgcgcgag cggccgctag aagcgcgcga tgcctcagcc aaggcgcacg
    27481 agcgttttac cgacaagcag tccgatttcc ttgcctatct gaacatttgg gacagcttcc
    27541 agcgcgaacg cgataaaggc ttgtccaaca agcagctggt gcagtggtgc cgccaatatt
    27601 tcctgtcgca cctgcggatg cgcgagtggc gcgagctgca ccaccagctt gcccaaaccg
    27661 cgattgaaat gggtttaacc accaaggaag ccgctttcag acgacctccc gaagtcaggc
    27721 agctcacgtc gtctgaaaat gcgggtgacc aagacctatc tgctaaactc aaacaaaaac
    27781 aactggataa aaagcaacac cgcgcccaaa tccgcgccgc caaagaagcg ggctacgaac
    27841 aaatccaccg cgccctgctc actggcctta tcgccaacgt cggcatgaaa tcgcccgacg
    27901 gtaacgacta caccggcgcg cgcggcagcc gcttccacct tttccccgcc tccgccctgt
    27961 tcaaagccaa acccaaatgg gtgatggcgg cagaattggt tgaaaccacg cgcctttacg
    28021 cgcgcgacgt cgccgttatc cagcccgaat ggatagagca ggaagcgccg cacctcgtcc
    28081 gctatcatta tttcgagccg cattgggaac aaaaacgcgg cgaagtcgtc gccagcgaac
    28141 gcgtgacgct ttacggtctg accgtattgc cgcgccgccc cgtgtcttac ggcaaagttg
    28201 cccccgaaga agcgcgcgaa atctttatcc gcagcgcgtt ggtggcgcag gaatgcgatt
    28261 tgaaagcgga tttttttgtc cacaacaaaa agctgattaa agaaattacc gaactcgaac
    28321 acaaatcgcg caagcaagac gtgctggtcg atgacgaagc cctgtttgcg ttttataacg
    28381 aacgactgcc cgaaatggct tggaaagacg cgcaaggcag cgtttgggga agtgaagatt
    28441 ccgtacggat tattgaatct gacaaagccg agaggtcgtc tgaaaatgag cgcaacgagt
    28501 ttcgtaaaaa caagcgtaat gggtctcgcc aaaatgaaaa tcacggcaac accgtaggtt
    28561 gggttgaaaa cccaacatca gccgcaactg caaaaactgt tgggtttgac aatccaacct
    28621 acgctgccca acaaaccacc ccctcccccg tgggggaggg tcggggagag ggcaaaacag
    28681 ttgccgcaca aaccaacttt tccgcaaccg cagcaaaccc tctccctaac cctctcccgc
    28741 aggagaggga acagagtgcc gcagcttcaa cgatttcaga cgacctgcgt cctgcaaatc
    28801 tgcagcaaac cgccccctcc cccgtggggg agggctgggg agagggcaaa acagttgcca
    28861 cacaaaccaa cttttccgca acctcaacaa accctctccc gcaggagagg gaacagagtg
    28921 cctcagcttc aacgttttca gacgacctgc gtcctgcaaa tctgcagcaa ccctctccct
    28981 cccccgtggg ggagggctgg ggagagggca aaacagttgc cacccaaacc aacttttccg
    29041 caacctcaac actttcagac gactccaaac ccaaaaagca gcctgcaccc caaaaaaacc
    29101 gtctgaaacc cctacccctc gccgacatcc gcaccttcca agcctggctc aaaaccgccg
    29161 aacgcgacaa tctgcgcctg ctgttcctca gccgcgacga tttgatgcaa cacgccgccg
    29221 cacacattac cgaagaacag ttccccaaat tctggcaaac cgcagacggc aagttcaaac
    29281 tttcctaccg cttcgagccg caccatccgc tagacggcgt gaccatgacc gtgccgctga
    29341 ccgtcctcaa ccgcctgcac gcgccgtcgc tcgaatggct ggtgcccggc atgatacgcg
    29401 aaaaaatcca gttgcaaatc aaagcactgc ccaagcaaat ccgccgcatc tgcgtgcccg
    29461 tacccgaatt catcacccaa tttttaagcc aaaaccccga ccgcaacgcc cccatcctgc
    29521 cccaactcgc ccaagccatc gccaaaaccg caggcgacat ccgcatattc gagcaaatca
    29581 accaagacga atgggccgcg ttcaggctgc ccgaacactg ctatttcaac ctccgcatta
    29641 tcgacgacgg cggacaagag cttgccggcg gccgcaaact gcacgaattg caacaacaac
    29701 tcggtcaagc tgccgccgtt accttccgtg acaacaccca agaatttgag cgcgacaacg
    29761 tcaccgcatg ggacatcggc accctgcccg aatccatcaa attcgcacgc ggcaaacaac
    29821 agctcaccgg ctatctcggc ctacaaaaag aaaaagacgg ccgcatcgcc ctgcgcctgt
    29881 ttgataccac agaagccgca gagcaggcac accgtcaagg tgtcatcgaa ttgatgaagc
    29941 tgcaattaaa agagcaggta aaggatttga acaaaggcat ccaaggcttc acccaagctg
    30001 ccatgctgct caaacacatc aacgccgaca ctctgcgcga cgacctcacc caagccgtct
    30061 gcgaccgcgc ctttatcggc gaagacgagc tgccgcgcaa cgaaaaagcc ttcaaagaac
    30121 aaatcaaacg cgcccgcagc cgcctgcccg ccgtcaaaga agccctcagc cgctacctgc
    30181 aggaaaccgc cgccgtctac gccgaactca acagcaaact cggcaaacac ccattgaccc
    30241 accttctaag actacgcctg caaaccctgc tcgccgccgg cttcgccacc cgaaccccgt
    30301 gggcacaatg gccgcgcctc cccatctacc tcaaagccat gaccctgcgc ctcgaaaaat
    30361 acagcagcaa ccccgcccgc gacgcagccc gcgaagccga tatccaagag ctggaacaaa
    30421 tgtggcagga aaaaacagac agcctgatta aacaaggtct ccccatttca gacggcctcg
    30481 ccgcgtttaa atggatgatt gaagaattga gggtgtcgct gttcgcgcag gaattgaaga
    30541 caccgtatcc ggtgtcggtg aagcggctgt tgaaagagtg ggaaaaaatt gaaaaataaa
    30601 aaaacagcct gaaaagtttc aggctgtttt tttatttgac taatcgaagt ttcctatatc
    30661 tatttaagtc cctctcaact aatccaaaag ttaaatcagc aacatctttg ggggatacgt
    30721 ttaaattttc agcaatctgt tcaataccaa tgccatcatt ttttaaaata gtaagcattt
    30781 tacgtaatgc gcttgatatt tccctttcca ttggctctgg ttcgatagtt cgatattttt
    30841 tctttgcaaa caaaggacaa agattgtgta tatacatcct atcagtaatc attcctaatt
    30901 tatgcatccg atatgctaag gcaacaagtg atacaccaaa tcgtcttttg atttttaata
    30961 aattttcaat agtgatagga acatgacgat ataagcgtag tgcagcctcc ggcattaaaa
    31021 aagctgaagc aaaggcatta gcctcttttt cgataatatc acgaggttca tcttctgtaa
    31081 tttcactatt tttactatgt tccatactgt atttatcacg gattaagtgc cctaattcat
    31141 gggcagcatc aaatcgacta cgttctgcag atttttgtgt atttaaaaat acaaatggat
    31201 gattttcata ccaagtacaa aaggcatcaa tgtcctttgt atctaaagat aatgaaaata
    31261 cacgaacacc cttagcttca agtagggtga tcatattcgg aataggttca ttgccaagcc
    31321 cccattctaa tcttagttcc tgagcagcct cttcaggaga aatatcagaa aaatcaggca
    31381 atacggcttg acttagtgta aattctgtct cgagccagtc atttaacaaa aaagccgtaa
    31441 tgctatgatt taatgcttgt ttttcaagcc tcttcgaggt gcgtgaacga gcacgaaaac
    31501 ttactgcctg agatttcaac tcaggcagtc tttcgtcatt agtaaagaaa tgaactggaa
    31561 actctaataa attggctaat tcatttaaat caggtatttg ctcatctttt acatagtttc
    31621 taacctgtcg agcggtaata cctaataact cagctaattt tgtttgcgta caaccacgtt
    31681 tatccagcgc aaattccagt ctctcacgat taaatgtctg catgatttat cattcaaatt
    31741 atcctgcttt ttgttttctt tcaaccaaag gctcatattc ttcaacaggt tgcttacgtt
    31801 caagctcatc aaatttagtt aaatcaacat cagctaatat aattcgctgc ttgtacccag
    31861 ttatttgatg actaacaaaa ccactcggta aagataattc aagttgcact ttattatact
    31921 tccagtgaaa cagcagaacc caaaactgca cagtatcagg caaatctagt tttgaattac
    31981 gaatagcctc ctcaaatccc ttacctttcc ttgcggttgt cattggcatc ccgtgatgcc
    32041 taccaacatc tgaagtagca gtagccacaa taatactttt agttcgacat ggcgagagac
    32101 atagaaatgc accacccgac caaggctcaa gcgtccagcc atctttactt aaatataccc
    32161 ttagagcaaa tgtaatttct gcttgccgat acatccccaa tgtatttctg tcagataatg
    32221 ctgctttgtc ctgaatatta ttatgcgcag taagtacaat ctctttaagc atctcctgag
    32281 ataagtactt gctgatttca cttaaagcaa tatcactatt ttgttgctcg actatttctc
    32341 ctacttcaaa tgggaaaggt tctgataatg caaattccac cataaaaatt tcctaatttt
    32401 atacgtaatg tttacacaat atatcaggaa atatgaaaac gtacaactat atctataaag
    32461 caattaataa gtagcctgcc caaccgtgtc cttatctttc ggcacacccg acctgcaaat
    32521 cacgcaaaac ttggaatccg tgtgtagggt gtgtgcggta catacgcacg cagtcttttt
    32581 aaaccacagc ccttcccaac taaaccaaaa ggtcgtctga accctatttt cagacgacct
    32641 tttgccactt tgtaaaacaa atcttcccac catcctctcc ccaaacatcg cccgaaccag
    32701 taaacttctc atatttcaac aactccttgg aagcaaacca tgtctggtat ctacctaccc
    32761 cgcctattcc cgccccatat cgccgaacgc ggcctgttgt attttcagca gggcaaggtt
    32821 ctcgatgtcc gaaaaacttc cgccgggcat tatcgggcgg aggtgtgcgg ttcggaaaac
    32881 tattgggtat agttgaagct ggatagtgat ttgtatatta aagacgaagg ctgcaattgt
    32941 ccttatatct aagagtgcaa acatacctta aattactata ttgcataggc aaaatacaag
    33001 cctataacga attggaaaca aaatgccgtc tgaaaacatc ttcagacggc attataaaat
    33061 ctgttcacct tttcagatga gtaatgtaca cccttataca atttttgcta ctatgcccca
    33121 taaatccacg gctaaagata tccttattat gtcctatgat ttatcgaaac gacttgtaat
    33181 cggcttagca tcaagtgccc tattcgactt atccgaatcg gataatatat ttagaatgga
    33241 aggggcagaa acctataggc aatatcagag agaaaaacaa aaccatcccc taaaaaaggc
    33301 gttgtctttc catttattaa aaaacttctg tcaatcaatg aaataaaccc aaacgaccca
    33361 acgattgggt ttattctttt atccagaaac aatccagata cagattacga gtcataacta
    33421 taggcttaat attacacgat tctcattcca tcaaggcgga aaaccgcaca aatactgaaa
    33481 cactatcgat cgatttgtaa acaagcctac ttaagtaact tgcagtcctt atcatttcct
    33541 ttaaaataat ccagcccgtc actacacgaa ctggcggact tcttgcaaat aaaggttact
    33601 agattttcat tcatcttaat aataaaagga tttttatctt tatctatggc taccgccttc
    33661 aacatgaatt tactgtctaa agccccgcgc gcgattccat tcaaacggat acaaaagcct
    33721 tctgcctctt taatcggcaa acttggccac ttggtagatg tttgtttaaa cctcccattc
    33781 tgcagataaa acttttccat aaaatgtgca ttttctaaca aggctgcccg cactgcattt
    33841 atctttgctt tctcaacata attgcgatag ctcggataaa caattaaagc aagtacagac
    33901 aatatcaaga ccactgatat taattcaacc agcgtaaacc cccgattatc agtcattact
    33961 ttacttccaa taagaacaga ttattcaaca tatttctttg aacagactta ctatcccatt
    34021 caacagtatg catatttccc actctatttt ttagcggccg gtatagc
//
LOCUS       DAXHNE010000005        32175 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.005, whole genome shotgun sequence.
ACCESSION   DAXHNE010000005 DAXHNE010000000
VERSION     DAXHNE010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32175
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.005"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(242..2170)
                     /gene="rpoD"
                     /locus_tag="ACFM4Z_000169"
     CDS             complement(242..2170)
                     /gene="rpoD"
                     /locus_tag="ACFM4Z_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFC6020279.1"
                     /translation="MSRNQNHEEYQDDTRPLSIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLQWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            complement(2365..4137)
                     /gene="dnaG"
                     /locus_tag="ACFM4Z_000170"
     CDS             complement(2365..4137)
                     /gene="dnaG"
                     /locus_tag="ACFM4Z_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFC6020280.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQLYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITASALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQV
                     LEHMRGSPYEETINRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            complement(4115..4267)
                     /locus_tag="ACFM4Z_000171"
     CDS             complement(4115..4267)
                     /locus_tag="ACFM4Z_000171"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020281.1"
                     /translation="MPSEHPAPVSDGILPEPPHPTAIPKNPDFVPSVPKTDIPSAPHH
                     DSIRLH"
     gene            complement(4282..7032)
                     /gene="secA"
                     /locus_tag="ACFM4Z_000172"
     CDS             complement(4282..7032)
                     /gene="secA"
                     /locus_tag="ACFM4Z_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFC6020282.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADEALSDEEKQAQISALEDGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMKEIR
                     SDVVSDLMDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            7163..7585
                     /locus_tag="ACFM4Z_000173"
     CDS             7163..7585
                     /locus_tag="ACFM4Z_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HFC6020283.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLILLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESLDSAAAKLEKRHPELAEALANLVRRHGA"
     gene            7866..8417
                     /gene="azu"
                     /locus_tag="ACFM4Z_000174"
     CDS             7866..8417
                     /gene="azu"
                     /locus_tag="ACFM4Z_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HFC6020284.1"
                     /translation="MKTYLALISAAVIGLAACSQEPAAPAAEATHAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEEASLTLDPAKLADG
                     EYKFACTFPGHGALMNGKVTLVD"
     gene            8512..9021
                     /locus_tag="ACFM4Z_000175"
     CDS             8512..9021
                     /locus_tag="ACFM4Z_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC6020285.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQIAQYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYVPDEEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            9055..9675
                     /locus_tag="ACFM4Z_000176"
     CDS             9055..9675
                     /locus_tag="ACFM4Z_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFC6020286.1"
                     /translation="MLTPKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPEYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQNESDR
                     KLQNDEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGK"
     gene            9789..10934
                     /gene="dapE"
                     /locus_tag="ACFM4Z_000177"
     CDS             9789..10934
                     /gene="dapE"
                     /locus_tag="ACFM4Z_000177"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFC6020287.1"
                     /translation="MTETQSLELAKALISRPSVTPDDRDCQKLLVERLYKIGFAAEEL
                     HFGDTKNIWLRRGTKVPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELSVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTDVARAAIAEICGVETELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLNDIPKLSAVYEGILARLLAGNAV"
     gene            complement(11368..11520)
                     /locus_tag="ACFM4Z_000178"
     CDS             complement(11368..11520)
                     /locus_tag="ACFM4Z_000178"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020288.1"
                     /translation="MTSNVCPPANATTSPAAILNSRPAHRNFLHPRRTISTAGSYTAT
                     LRYKAV"
     gene            complement(11534..11713)
                     /locus_tag="ACFM4Z_000179"
     CDS             complement(11534..11713)
                     /locus_tag="ACFM4Z_000179"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020289.1"
                     /translation="MRHFFIRQHRRRIIKRRAHPPGVRQLHRIAHCLRRPVLCGEPFY
                     GFLFESNPLRLIEVV"
     gene            complement(11674..12197)
                     /locus_tag="ACFM4Z_000180"
                     /pseudo
     CDS             complement(11674..12197)
                     /locus_tag="ACFM4Z_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
     gene            12547..13299
                     /locus_tag="ACFM4Z_000181"
     CDS             12547..13299
                     /locus_tag="ACFM4Z_000181"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFC6020290.1"
                     /translation="MITLPSLNNLPSKWDEIRHWLESRVFECGVMPHPNLTECEAKQF
                     ERDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGSRGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALEHGEVQTALF"
     gene            13491..13566
                     /locus_tag="ACFM4Z_000182"
     tRNA            13491..13566
                     /locus_tag="ACFM4Z_000182"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13524..13526,aa:Asn,seq:gtt)
     gene            13697..14707
                     /gene="waaF"
                     /locus_tag="ACFM4Z_000183"
     CDS             13697..14707
                     /gene="waaF"
                     /locus_tag="ACFM4Z_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HFC6020291.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENSFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAIIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLDIGKPVLAFCPGAEFGPAKRWPTRHFAELGKHYLAAGWQVWLFGS
                     QKDDEIAEEINRLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            14762..15208
                     /gene="smpB"
                     /locus_tag="ACFM4Z_000184"
     CDS             14762..15208
                     /gene="smpB"
                     /locus_tag="ACFM4Z_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFC6020292.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            15574..17608
                     /locus_tag="ACFM4Z_000185"
                     /pseudo
     CDS             15574..17608
                     /locus_tag="ACFM4Z_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(17789..19156)
                     /locus_tag="ACFM4Z_000186"
     CDS             complement(17789..19156)
                     /locus_tag="ACFM4Z_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFC6020293.1"
                     /translation="MLDLHTEFSRLLPADEIAEPSPTLLKDQRNRFTSAPDIILQPLS
                     VKSVQTIMRFCHQHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFANPLDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKLPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAVPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINSTVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(19266..19532)
                     /locus_tag="ACFM4Z_000187"
     CDS             complement(19266..19532)
                     /locus_tag="ACFM4Z_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020294.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPAAEAPAAEAPAAEAPAA
                     EAPAAEAAATEAPAAEAAATEAPAAEAAATEAPAAEAPAAEAAK"
     gene            20005..20487
                     /locus_tag="ACFM4Z_000188"
     CDS             20005..20487
                     /locus_tag="ACFM4Z_000188"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6020295.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            20574..21386
                     /gene="hpnC"
                     /locus_tag="ACFM4Z_000189"
     CDS             20574..21386
                     /gene="hpnC"
                     /locus_tag="ACFM4Z_000189"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HFC6020296.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLQRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYGNFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LNGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKKALWK"
     gene            complement(21459..21929)
                     /locus_tag="ACFM4Z_000190"
     CDS             complement(21459..21929)
                     /locus_tag="ACFM4Z_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HFC6020297.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            22020..23861
                     /locus_tag="ACFM4Z_000191"
     CDS             22020..23861
                     /locus_tag="ACFM4Z_000191"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFC6020298.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFKIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPRAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            complement(24272..25468)
                     /locus_tag="ACFM4Z_000192"
     CDS             complement(24272..25468)
                     /locus_tag="ACFM4Z_000192"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC6020299.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     IFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            25819..26118
                     /locus_tag="ACFM4Z_000193"
     CDS             25819..26118
                     /locus_tag="ACFM4Z_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020300.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEDGAGN
                     HVLSPFAQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNI"
     gene            26118..26303
                     /gene="ccoS"
                     /locus_tag="ACFM4Z_000194"
     CDS             26118..26303
                     /gene="ccoS"
                     /locus_tag="ACFM4Z_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HFC6020301.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKEKENDVR"
     gene            26293..27603
                     /locus_tag="ACFM4Z_000195"
     CDS             26293..27603
                     /locus_tag="ACFM4Z_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6020302.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHKPVFTAIIGISWFWFVGAIYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKEP"
     gene            27761..28495
                     /gene="dnaQ"
                     /locus_tag="ACFM4Z_000196"
     CDS             27761..28495
                     /gene="dnaQ"
                     /locus_tag="ACFM4Z_000196"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFC6020303.1"
                     /translation="MIQRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIEVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGEAAEEKMETKPVVHTETKRSGKLKVIRADENELAAHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            28492..29181
                     /gene="ispD"
                     /locus_tag="ACFM4Z_000197"
     CDS             28492..29181
                     /gene="ispD"
                     /locus_tag="ACFM4Z_000197"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFC6020304.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEGVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLEIGLASAD
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAIPVADTLKRADGGNISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGVRPLLIQGDVRNLKLTQPQD
                     AYIVRLLLDAV"
     gene            29213..29695
                     /gene="ispF"
                     /locus_tag="ACFM4Z_000198"
     CDS             29213..29695
                     /gene="ispF"
                     /locus_tag="ACFM4Z_000198"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFC6020305.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            29772..30443
                     /gene="rpiA"
                     /locus_tag="ACFM4Z_000199"
     CDS             29772..30443
                     /gene="rpiA"
                     /locus_tag="ACFM4Z_000199"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFC6020306.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDKPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            30512..31183
                     /locus_tag="ACFM4Z_000200"
     CDS             30512..31183
                     /locus_tag="ACFM4Z_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFC6020307.1"
                     /translation="MQIKKIMKWFPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            31199..31378
                     /locus_tag="ACFM4Z_000201"
     CDS             31199..31378
                     /locus_tag="ACFM4Z_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020308.1"
                     /translation="MNGKCRLKTARPAFLRRRATAVCRDKKVQAAVFTVKWRIILQRR
                     QFRRFLIRTVYGFSF"
     gene            31362..32108
                     /locus_tag="ACFM4Z_000202"
     CDS             31362..32108
                     /locus_tag="ACFM4Z_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC6020309.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
BASE COUNT         7319 a         7552 c         9159 g         8145 t
ORIGIN      
        1 tgctcgatcg gctgccaatc aatcacccgg tccaacttca atagcgggaa acggtcgatg
       61 tgtttggcaa tcatggcttg ggcggtttgt tggaagaagg tgctcctaaa tgtcttggtg
      121 ggaatttagg ggattttggg ggattttgca aaggtctcgg gataggagtg tgggatatgt
      181 ggaagcgttg tttattgtgt aagaaaaaac cgcaggtatt tgaaacctgc ggttttttgg
      241 tttacagctt gctgtcttcg ctgtccaaga aacttctcaa acggtcgctt cttgtcggat
      301 gccgcagctt gcggagtgct tttgcctcga tttgtcggat gcgttcgcgc gttacgtcaa
      361 actgtctgcc gacttcttcc agcgtgtggt cggtgttcat atcgatgccg aaacgcatac
      421 gcaggacttt tgcctcacgc ggtgtcaggc tttcgaggat ttctttggtt acttcgtgca
      481 ggctggtgta cattgccgca tcggccggcg caacattgtt ggcatcttcg atgaagtcgc
      541 ccaagtgcga atcgtcgtcg tcgccgatgg gggtttccat cgaaatcggc tctttggcga
      601 ttttcatgat tttgcggatt ttgtcttcgg gcatctgcat cagttcggca agtttggcgg
      661 aatcgggttc ttcgccggtt tcttgaaggt gttggcgcga gatgcggttc atcttgttga
      721 tggtttcaat catatgtacc ggaatgcgga tggtacgcgc ctgatcggca atcgaacggg
      781 tgattgcctg gcggatccac caggttgcgt aggtggagaa tttgtagccc cggcggtatt
      841 cgaacttgtc gaccgctttc atcaggccga tgttaccttc ctgaatcaaa tcaaggaact
      901 gcaagccacg gttggtgtat tttttggcaa tggaaatgac gaggcgcaag tttgcctgaa
      961 tcatttcctg tttggcggct gcggtttctt tttcgctcga caccatattt ttgttgattt
     1021 ctttcaactc ttcgatggaa atgcgggttt ctttttccat atctgccaac tcggtttgtt
     1081 tttcgaggat ggcgtggcgg aagcggtcga gcgcgtcgct ccaaaccctg cctttggcga
     1141 tttcttcttc aatccattgc agattggtga tttcgggcag gaagttttgg atgaagtagt
     1201 cgcgctccat atggacgcgg tcgaggcaga tgtcgcggat ttcgcgttcg agtttgcgga
     1261 tgttttctac ttttccgcgc aggctgctgc tgaggctgtc gatttgccgg gtggcgaaac
     1321 ggacttccag cagtttgttg gcaatcgcgt cgcggtaggc gagatagtct ttgtgccggc
     1381 tgtggtgttt ttccaaacga ccgatcattt ttttgtagtc tttttcgatt tgggcaaagt
     1441 ggccgatgac tttttgtttc aattcggcga gattggctgc cgagatttca tccgcatctt
     1501 cttccgattc ttcgtcgtct tcgttttcgt ccgaattgtc gttggaaggt ttctcgggtg
     1561 ctgtggtttc caagtgcccc aagcccaatt cgttgagcaa tacttcattc gggtcgataa
     1621 tggcttctac gacttcgtcg acgcggattt cgtctttgcg gattttttcg atgagttcta
     1681 agatttcagc aatggatccc gggcaggcgg agatggcctg aaccatattt ttcagggcgt
     1741 tttcgatttt tttggcgatg atgatttcgt cttcgcgggt cagcaggtcg acctgtccca
     1801 tttcgcgcat atacatacgg acggggtcgg tggttctgcc gaactcggaa tctgcactgg
     1861 aaagggcggc ctcggcttct tcgacggcat catcgtcggt aacggcggca ttgtcgctta
     1921 acaatatgtc ttccgcatcg ggggcgtgtt cggtaacttg gatgcccaaa ccggaaatca
     1981 tgctgacgat attgtctatt tggtcggcat cagacatatc gtctggcagg gcgtcgttga
     2041 tttcggagta ggtgatgtag ccgcgttctt tacccatgat gatgagctga cgcaggcgcg
     2101 cgcgttgctc ttcaatgctt aacggacggg tgtcgtcttg atattcttcg tgattttggt
     2161 ttctggacat tgtctgcttt ctgttggtaa gatgccgacg gtttgcgttt ggacgcacct
     2221 gaaggcgtgg cagagtaggg tactctgatg ccgtctgaag gcttgaaggc ttttggggat
     2281 gggtgttctg caggtgtcgt tgttgcagcc ggggtgaatg cttttcagac ggcataacgg
     2341 tttacggatg gcggaatccg ccggtcaatt ttgttttgcg gtcagcagcg acagcaaaag
     2401 ttttttctcg ctttcattta agccggattg caggcttttt tgttttaatg tttcgatttg
     2461 gctgtatttt aactcattga gcagtttttt gatgccgatt tggaagtttt cgcaatcttc
     2521 ttcgccgccg ccttccattt cttccgattg aagggccgac cggaagatgc ggttgattgt
     2581 ttcttcgtag ggcgaaccgc gcatatgttc caaaacctgt gcggttgccg gcacggaagg
     2641 atggttttta atggtttcgg cgaggttggc aaggcaggcg aaatcgccgt ccaacgccag
     2701 ataatcgggc aggtctatat atgcagccca atccggattt atcaagaggc tgcggatttg
     2761 ccgttgcacc aatgtcggca taacgggctg tttgacggaa atcggcggca gtttgtagtt
     2821 tttttgtttg acgtgccgct tcggtgcttc ctgtcccagc agttgcgcga ggttgtcggg
     2881 gtcgatgccg accagttcgc taagccgttg ttttaacaaa taagccaatg ccgacgcggt
     2941 aatctgcgcc aaaagcggcg aactggtttt taccaattcc gccttgcctt cctgcgtatt
     3001 gagatgaatg ccgtctgaaa ggtgttccca gaaatattcc gacaagggct tgctttgatt
     3061 caacagcgcg tcttcaaatt gcgctttgcc gtaggcgcgg atgtagctgt cggggtcgtg
     3121 ttcttccggc aggaacaaaa aatgcagcga tttgtcgtcc ttcaattgcg gcagcgcgtt
     3181 ttccagcgcg cgccaagccg ctttgcgccc cgcgctgtca ccgtcgaaac agaaataaat
     3241 actgtccgcc tgacgcatta ggattttgac gtgttcagct gttgttgccg tgcccaaggc
     3301 cgccacaccg tagcccacgc cgaactgtgc cagcgcgacc acgtccatat agccttcgac
     3361 caccagaatc cgctccgctt ccttgacagc ggcacgcccc tcatacaagc cgtaaaggtt
     3421 tttccctttg tcaaacaaag gtgtatcggg agagtttaga tatttgggtt tggagtcgtc
     3481 cagcaccctg ccgccgaaac caattacctg cccgcgcgga ttgcggatgg ggaacataat
     3541 ccgatgacgg aatcggtcgt aataccgccc ctcattgtca atcaccatac ccgtatccac
     3601 caacgcggta ttcggatata gttggaacac ttgcgccaaa ggctgccagc cgtcgggcgc
     3661 gtagcccaaa ccataatgcg cgatgacttc cgcgctcaag ccgcgcttgt ccaaataggc
     3721 ttttgccgct ggattgaatt ttagctgttg cgcataaaaa tcagctgccg cagccgtcgt
     3781 ttcctccagc gtctgctgtt ttttcttgcg ctcggcacgg acttcgggat tatcgttttg
     3841 cccgcgcact ttaggcacaa tcatacccac gcggtcggca aggaactgca ccgcctccgg
     3901 aaacgacagc ccctgatgtt ccatcacaaa accaatcgcc gaaccgtgcg ccccgcaact
     3961 gaaacagtgg taaaactgtt tggtcggact gaccgaaaac gacggcgttt tttccttgtg
     4021 gaacgggcaa cacgccatat agttcgcccc gccttttttc agcggaacct gctcgtcgat
     4081 aatatcgaca atatcggttt tggctaaaag ctcgtcaatg aagtcggatg gaatcatgat
     4141 gtggggcgga cgggatgtct gtttttggta cggacggtac gaaatcggga tttttcggaa
     4201 tggcagtcgg atgtggcggt tcaggcaaaa tgccgtctga aacgggagcg ggatgttcag
     4261 acggcatttg cgttcaaacg cttaagccag tttgccgtgg cattgtttgt atttcaaacc
     4321 gctgccgcag gggcaggggt cgttgcggtg gacgatttgt ccccgcgctt ccaaggcttc
     4381 ggggctgaaa tccgttccat cgggattaaa ggcctcggta accaaatcgg tttgcgactg
     4441 acccaaaagt tcttccatat cgggcgattc ggaatggatg gactggatgt tgccgatggg
     4501 ttgctcttca accaccgcga cagggttttg ttcgatttga accgaggtaa gcagggaggc
     4561 aatatggaat ttgatgccgt tccacaggtc ttggaacatg gtaaaggctt cacgtttata
     4621 ttcctgcttc ggattttttt gggcatagct gcgcaggtgt atgccttgtc gcaggtagtc
     4681 catagcggcg aggtgttcgc gccattggtt gtcgatgacc tgcaacatca cgttgcgctc
     4741 gaaatcggcc attgcctgct taccgaccag ttcggttttg gcggcatatt cgttttcgat
     4801 gcgttcgatc aggcgttctt tgatgtcttg accgtcaatc gcattgtccg ccttcagcca
     4861 ggattggatg tcttcgtgca gtctgaattc ggcagccaga cggttctcca aagtcgggat
     4921 gtcccattgt tcttccatgc tgtcgggcgg catataggta tccatgaggt cgctgacgac
     4981 atcagaacgg atttccttca tcaggtcgct gatgtctttg ctggtcagaa tttcgttgcg
     5041 ctggctgtaa atgactttac gttgctcgtt ggcaacgtcg tcgtattcca aaacctgttt
     5101 gcgcatatcg aagtttctgc cttcgacttt gcgttgcgcc ccttcgattt ggcgcgtcag
     5161 caggttgtgc tcgatggcaa cgccgcgttc gggggcgagg cggttgagga tggcggcggc
     5221 gcggtcgagt gcgaataggc gcagcagcgg gtcttcaaac gagaggtaga aacggctgga
     5281 tccggggtcg ccctgacggc cggaacgtcc gcgcaactgg ttgtcgatac ggcggctttc
     5341 gtggcgttcc gtaccgatga tgtgcaaacc gcctgcttcc atcactttgt cgtgttctgc
     5401 ctgccagccg tcttcgagtg cggagatttg tgcctgtttc tcttcgtcgc tcaaggcttc
     5461 gtcggcacgg atggcatcgg tttggtgctt caggttgccg cctaaaacga tgtccgtacc
     5521 gcgtcccgcc atattggtgg caacggtaat cgcgccgact ttgcctgcct gggcgacaat
     5581 cagcgcttcg cgttcgtgtt ctttggcgtt gaggacgttg tgtggcagtc cggctttttg
     5641 cagaaggtgg gagaccagtt cggagttttc gatgctggtc gtaccgacga ggacgggctg
     5701 cccgcgtttg tggcattctt cgatgtcttt gacgacggct tcgaattttt cttcggcgga
     5761 acggaaaatc tggtcgttga agtctttgcg ctgtacaggg cggttggtcg ggatgatgac
     5821 ggtttcgagg ttgtagatgc tttggaactc gaaagcttcg gtatcggctg tgccggtcat
     5881 gccggagagc ttggtgtaca ggcggaaata gttttggaag gtgatggagg caagggtttg
     5941 gttttcgcgt ttgatttcca cgccttcttt ggcttcgacg gcttgatgca gaccctccga
     6001 ccagcggcgg ccggacatca gccggccggt gaattcgtcc acgatgacga tttcgccgtc
     6061 ttggatgacg taatgttggt ctttgtggaa gagggaatgc gcgcgcaatg ccgccataag
     6121 gtggtgcatc agggcgatat tggcggcgga atagagggag tcgttttctg ccagcaatcc
     6181 catttgggtc aggatttgct cggcgtgttc gtgacctgct tcgctcagga tgacctggtg
     6241 cgccttttca tcgacccagt agtcgccttc gccttcttct gtctcttggc ggacgaggtg
     6301 gggcggaacg gtgttcatga tttggtacaa ctggatgttg tcgtctgcct ggccggagat
     6361 aatcagcgga gtgcgcgctt cgtcaatcaa gatggaatcc acttcatcga caacggcaaa
     6421 attcaattcg cgctgcactt tgtcgtattg gtcggtaacc atattgtcgc gcaggtagtc
     6481 gaagccgaat tcgttgttcg taccgtaggt gatatcggcg gcataggcgt tttgacggtc
     6541 gaacggctgc atatctgaaa taatcacgcc cacggtaagg ccgaggaaat tgtagagcgg
     6601 ctccataatg cccgcatcgc gtgaggcgag gtagtcgttg acggtaacga cgtgtacgcc
     6661 tttgccggcc agcgcgttga gatagacggc gagggtggcg accaaggttt tgccttcgcc
     6721 ggtacgcatt tcggcgattt tgccgtcgtg cagcaccata ccgccgataa gctgcacgtc
     6781 gaagtggcgc ataccgaggg tgcggcggga cgcttcgcgg cagacggcga aggcttcggg
     6841 caaaatgccg tccaaagtct gaccgtcggc gaggcgttgt ttgaattcgg cagttttggc
     6901 ttgcagatca gcatcgctta gggcttgcat ctgttcttcg agcgcgttga ttctggcaac
     6961 ggatttacgg tattgtttca gcaagcggtc gttgcggctg ccgaagattt tcttggcaat
     7021 gtttgtcagc atgaataatt cctgcgcctg atataatagt gggcgaaaac gcgattttaa
     7081 cacaagcgtt tgtgcaaagt gctaatgccg tctgaaataa aggcgcgttt gcggtatttc
     7141 aatctttttg ggtgtatgtt ttatgaattt ggaacagttg ggcaggcggg acgcgctgct
     7201 ttccggactt ttgaaacagg cgggacagtg gcggcggttg gatgccgccg tgaaaaaact
     7261 gctgcctgcc aacctgcacc cgcattttca gacggcatgt atcgaagacg gtaggctgat
     7321 tcttttggcg gcaaacaata tggcggcatc gcgtttgaag atgattgccc cgtcggtatt
     7381 gccgcagttg gcagggcttg atgcttcgat acgctctgtt tcggtcaggc tggtcccgaa
     7441 accggaaaaa ccgccgaaaa ccaataccct gcatttgagt aaggctgcgc tggagagttt
     7501 ggattcggcg gcggcaaagt tggaaaaacg gcatcccgaa ttggcggagg cattggcaaa
     7561 cttggtcaga aggcatggcg cataaaatgt atacgggaat ttgtgtaaac atccgttaat
     7621 attaagaagt aaaggataat gggtctaata ctaaagaaat aggttcgggg taaaattgcc
     7681 ccttttaaag taaacgattg taaacttgca gacaggcttt gatttcaaat gaaatttgta
     7741 gcaaaatgcc gccccgaaac atctgtttgt gcaacgcggc ggaatctttt tcaaggtttt
     7801 gttaatggcg gttgcacttt gatttccgta aaaccgaata ttattttatc gattggagat
     7861 ttgttatgaa aacgtatctg gctctgattt ctgctgccgt tatcggtttg gctgcctgct
     7921 ctcaagaacc tgccgcgcct gctgccgagg caactcatgc tgctgaagca cccgcttccg
     7981 aagcacctgc cgccgaagct gctcctgcag atgctgccga agcccctgct gccggcaact
     8041 gtgcggcaac tgtcgaatcc aacgacaata tgcagttcaa caccaaagac atccaagtca
     8101 gcaaagcatg taaagagttc accatcactc tgaaacatac cggtacgcaa cccaaagcca
     8161 gcatgggtca caaccttgtg attgccaaag ctgaagacat ggacggcgta tttaaagacg
     8221 gcgtaggtgc tgccgatacc gactatgtca aacctgacga tgcgcgcgtt gttgcccaca
     8281 ccaaactgat cggcggcggc gaagaagctt ccctgacttt ggatcctgcc aaactggctg
     8341 acggcgaata caaattcgct tgtaccttcc caggtcacgg tgctttgatg aacggcaaag
     8401 tgactttggt cgattaatcc gcttaaagtc tcaaaagacg gacagcctgc tttgtgcagg
     8461 ctgttttatt ataaaatgac tgcttgaaaa gtgcccgttg agaacgaaaa catgaatccg
     8521 tttgaaacca aaagcgttac ctttgccgaa ccgattgaaa tgctgtatgc ctgccacggc
     8581 aaagtgcgcc gtttttgcgg acaagtcgcc atgctgtcgg actatatcgc cgaaaacggc
     8641 tgcaatcaga ttgttttgca aaccatccgc caaatcgccc agtatttcaa cgttgccgcg
     8701 ccgctgcacc atgaagacga agaagaaaac ttcttcccgc tgctgctgca atatgcgccg
     8761 caagcccaag aaagcgtgga cgagcttttg cgccaacata tcgggctgca cgacaactgg
     8821 gcggctgttt ccgccgaatt tgccaaactc gaagcagaca acgcttatgt ccccgatgag
     8881 gaagcgttca aacgttttgt ggcgggatat gatgttcatt tggcgattga agagccgctg
     8941 tttgatatgg gcaacacctt tatcccaaaa gaaaaactga ccgaaatcgg cgaaattatg
     9001 gcggcgcgcc ggcgcaaata atgccgtctg aaacaatcgt ctcaaggata tattatgctg
     9061 accccgaaaa gttgcgattt gttcaatatc ccgtttttcc aattttccca gcttaaaaaa
     9121 taccagcctg aaagcattcc gcaaatcaag gctgactata aagaaaactg gcagatatgg
     9181 cagcagctga ttcagcaggt tgccgccgaa ttgggtgcgc cgtttgcgcc gccgcacatc
     9241 gaacgctggt gtaacggctg gcaggttcgc gcccatttct ttgcctactt caaatacgaa
     9301 caatataaaa attccgccgc gattttatcg attttattga accgccgccg cctgagcgtc
     9361 agcttggatt ggcactgcta caaagccgac gtgtcgccga ttgcgctgcc cgaatacaac
     9421 cgctggttgg ataattttga taccgaaaaa tacgcttctt tcgatatgtg gcacggtgcg
     9481 gaaagcgaat acgacgacta ccgcaccgtc gcgcaacaaa acgagtccga ccgaaagttg
     9541 caaaacgacg aagacttttt ctgcatcggc aaacacatcg aacgcgacga tttgggcagg
     9601 caggatgtcg cgaaatggat agcggaaacg gtggaagatt tgctgcctct ttacgaagcg
     9661 tgtcacggca aatgattcag aagactgacg ggtaaaaata cccaccccgt atgccaacct
     9721 aacctaaatg ccgtctgaaa atcacattca gaacgtttca gatggtctaa tttcaaaagt
     9781 gaacaaatat gaccgaaacc caatccctag aactagccaa agcgttgatt tcccgcccat
     9841 ccgttacccc cgacgaccga gattgccaaa aactgcttgt cgaacgcctg tacaaaatcg
     9901 gttttgcggc tgaagaactc catttcggcg acaccaaaaa catctggttg cgacgcggca
     9961 cgaaagttcc cgtcgtctgt tttgcagggc ataccgacgt tgtaccgaca ggccctgttg
    10021 aaaaatggga ttcgcccccg ttcgagccga ccgaacgcga cggaagatta tacgggcgcg
    10081 gcgcggcgga catgaaaacc agcatcgcct gcttcgttac cgcctgtgaa cgcttcgttg
    10141 ccgaacatcc cgaccaccaa ggcagcattg cgcttctgat tacttccgac gaagagggcg
    10201 acgcgttgga cggcacgacc aaagtcgtcg atgtattgaa agcgcgcggc gagttgattg
    10261 actactgcat cgtcggcgag ccgaccgccg tggacaaatt gggcgatatg attaaaaacg
    10321 gccggcgtgg ctcgctgtcg ggcaacctga ccgtcaaagg caagcaaggt catattgcct
    10381 atccgcattt ggcaatcaat cccgtccata cttttgcccc ggccttgtta gagctgacgc
    10441 aggaagtctg ggatgaaggc aacgaatatt tcccgccaac cagctttcaa atttccaata
    10501 tcaacggcgg cacaggcgcg accaacgtca ttccgggcga gctgagcgtc aaattcaatt
    10561 tccgcttctc caccgagtcc accgaagcag ggctgaaaca acgcgtccac gccattttgg
    10621 acaaacacgg tgtgcaatac gatttgcagt ggtcgtgttc ggggcagccc ttcctgaccc
    10681 acgcgggcaa actgaccgac gtggcacgcg ccgccattgc cgaaatctgc ggcgtagaga
    10741 ccgaattgtc caccaccggc ggcacttcgg acggacgctt catcaaagcc attgcaaaag
    10801 aactcatcga attaggcccg tccaatgcga ccatccacca aatcaacgaa aacgtgcggc
    10861 taaacgacat tccgaagctg tctgcggtgt atgaggggat attggcgcgg ttgttggctg
    10921 gaaatgccgt ctgaaagcag cttgcttgcc ggatatggat gtccgaccat ttgcttagac
    10981 ctaacgtggg tttgagataa cacattgatt ttttatgtag agcgatttat ttcaacggca
    11041 atccattccc tctcccgtgg gagagggtta gggagagggc atcggggcgg taacggaaat
    11101 attgtctgta tctgccccgt tttaccctct ccccaaccct cccccacagg ggagggagtc
    11161 aagttgcagg cacaccgacg gaatatggtt gaaacccggc cattcggggc agtgggattt
    11221 tggtttattt ttgggaagta accccttcca aatcagggta aacagggctg cgccttatgt
    11281 gcggccctgc gtgttggaga taatagtgcc gtcaaattac tttcatacgc gcgaacgatt
    11341 gggtgctttg ggcggcatcg tgcaacatca taccgccttg taccgcagcg tggcggtata
    11401 tgaaccagct gtgcttatcg tgcggcgcgg atgtaagaag ttgcggtggg cggggcgcga
    11461 attgaggatt gcggcgggtg aggttgtcgc gttggcaggc gggcagacgt ttgacgtcat
    11521 caatattcct gattcagacg acctctatca ggcgcaatgg attgctttcg aacaggaaac
    11581 cgtagaaagg ttcgccgcac aatacgggac ggcgcaggca gtgtgcgatg cggtgaagct
    11641 gccgcacccc gggcggatgg gcgcggcgtt tgattatgcg gcggcggtgc tgacggatga
    11701 agaagtgccg cacaatgcgg cggaggccgc gctttgcggc gtgttggcgt ggttgcagca
    11761 tgacggcatc ggttttgccg tgtacgaagg cgtcaacctg atgcggcaaa tccgcaaact
    11821 gattaccgcc gatatggctg cggacgggtc gtcggcgatg ctggcgcagc ggcttaattg
    11881 cagcgaggcc gcattgcggc ggcggacatc ggcgggaatg tcggcgacca cccagtgcat
    11941 ccagcccagt ccggtcggcg cgtctttatc gtaaacggtc aggacgaaac ttttgtcccg
    12001 gcgggcggat ttttccacga cagcgcgggc gaagcattgt cgcctgaaca gcaaaagccg
    12061 taaggcgcgc tcaaaagctg gttttgcgtg aagccgccgt cttcggacgg gttgtcgaat
    12121 tgcagcgtga atgcgccccc ggcaaaggca gcggttgaaa aagccagcag tacggcggag
    12181 aggatgaggg tttgcataga tgattccttt gcttgatgaa taagtggatt cattataggc
    12241 tgtcaacggc ggcaggaatg tgccgaaacg gttggatgat gtgccagaat gcgcaaatgc
    12301 aaaaatcgtg gtttcagacg accctttgca ggtcgtctga aatttttcat ggggcggcgc
    12361 tttatgtgtc gtcctgtgtg ttgaaacatt cctttctctg atgctttaga aatatcgaat
    12421 atttaggtat tgccgcaacc taaccctcaa gatttacgtc atagcccgtt cctgctccgg
    12481 cgtgcgggaa tggcaaaaat tggggatttt gccgtcaaat ctatgatagg gatacggagc
    12541 aaacaaatga ttaccctgcc ttccttaaac aatctgccgt caaaatggga tgaaatccgc
    12601 cattggctcg aatctcgggt ttttgagtgc ggcgttatgc cgcatcccaa tctgacggaa
    12661 tgcgaagcca aacagttcga gcgggatttt tgggacaata tcggctgcgc gcctgaagaa
    12721 tatgtccgca tccgccgcgc catccgtctg ttggaagcgc gttatcccga cagtttgaac
    12781 gagctggtgt gtgccgccat tgccacgcct ttgggcgaga tgctggcggt gttcggtagc
    12841 aggggtttgt gtttgctgga atttgtcgga cagaagcatt tggaacagga aattgccgcc
    12901 gtccaaaaag ccttgcgcgg acggtttgtg tttcgggagg atgagcggac gcaacttttg
    12961 cggcaggaat tggacttata cttcaagggt cgtctgaaaa cctttgccac gcctttggag
    13021 cagattggca ccgagtttca aaagcaggca tgggacgcgc tcttggcgat tccttacggc
    13081 gaaacgcgca gctacaagga gcaggcgcag cgtttgggca accccaaagc cgtccgcgcc
    13141 gttgccgccg ccaacgggca gaacaaagta tctatcctga ttccctgcca ccgcgtcatc
    13201 ggcagcgacg gcaaactgac cggctacgcg ggcgggttga accgcaaaca gtttttactg
    13261 gctttggaac acggcgaagt tcagacggca ttgttttaag cggggggagg gcagaacggc
    13321 tcatcgggct taaatatggt gtgatgacgg ctgtggaaat tcatacaacg gcaatccttt
    13381 taaggcatcg gtcttaatgt ctttctgatt catatgcttg tcgttttgtc gcccattttt
    13441 ccaaaattaa ggattgacac attttttccg gacgctataa tacgccccat tccccgatag
    13501 ctcagtcggt agagcgacgg actgttaatc cgcaggtccc tggttcgagc ccaggtcggg
    13561 gagccagaat tttgaagccc gaacttgaat gttcgggctt tttcatattt gcacgtccgg
    13621 cgttcggaat gcggcaagat aggttttcca acggtttcgc ggtaaaattc tgcctgtctg
    13681 atttcggttt cttgttatgt ccatcaaaat cctgattatt tcccccagtt ggataggcga
    13741 ctgcgtgatg acccagccct tgttccgccg tttgaaggaa cttcaccccg gttgcacgat
    13801 tgatgtgttc gcaccgaagt ggtcgatggc ggtgttcgag cgtatgccgg aagtgaatga
    13861 aattcttgag aattcgttcg gacatggtgc gttggagttg aaacgccgtt ggcgggtcgg
    13921 tagggatttg gggcggcgcg gatacgatca ggttatcgtg ttgcccggtt ctttgaaatc
    13981 ggcaatcatc gcgctggcga caggtatcgg taaaaggacg ggttatgtcg gtgaaagccg
    14041 ttattttctg ttgaacgata tacgcaggct ggataaggaa cgtctgcctt tgatggtgga
    14101 tcgatatacg gctctcgcgc atccgagtca ggaagatttt gacgggcatt cgggattccc
    14161 cgagttttcc attgatgaac ggcggcggga aatttctgtc gaaacctttg gcttggatat
    14221 tggaaagcct gttctggctt tttgtccggg tgcggaattc gggccggcaa agcgttggcc
    14281 gacaaggcat tttgccgagt tgggcaaaca ttatctggcg gcgggttggc aggtttggct
    14341 gttcggttcg caaaaagatg atgaaattgc cgaggaaatc aaccgccttt cagacggtat
    14401 gtgtgtcaat ttgtgcggca aaaccgattt gtcgcaggca atggatttgc tgtcgttggc
    14461 ggacacggtc gtgtgtaacg acagcggatt gatgcatttg gcggcagctt tgggcaggaa
    14521 ggtggtggcg gtttacggtt cttccagccc gacgcatacg ccgcctttga gcgatagggc
    14581 gaaaatcgtc agcctgcact tggaatgttc gccgtgtttt aagcgggaat gtccgctggg
    14641 gcataccgac tgcctcaaca ggctgtatcc cgagaagatt gtgcaggcgg ttgaagaggc
    14701 ggtatgatgg gttctgtttt cgggtttatg ccgtctgacg gcggatattg ataggatgtt
    14761 tatggctatt gcgaacaata aaaaagcatt tcacgatttt ttcattgaag accggattga
    14821 agccggtttg gtcttggaag gctgggaagt caaagcaatc cgcgccgcgc gcgtacagct
    14881 taaagagagt tatatctatt ggaaaaaaga cgcgttttat ctggtcggct gccatattac
    14941 ggctttgccc acagcctcga cacacattaa accagatgcc gtacgtccgc gcaagctctt
    15001 gttgaaccag tcggaaatca acaaattaat cggcaaaacc gaacgtgccg gctacaccat
    15061 cgtgccgctg gatttgcatt tttcacgcgg aaaaatcaag atggaaatcg gtttggcaaa
    15121 aggtaagaaa cagcacgaca aacgccagag tatgaaagag gcggattgga aacgcgagaa
    15181 acagcggttg attaagcata cgcgctgatt gtggaaacag tgcttcagac ggtatgggca
    15241 aggctgccgc ctgatgtgta aacgttctac acctaaacca gcatccgctt taatcagcac
    15301 ggcatgatgc cccaagcttt cgtcaaaggt ttgagcaatt tgatgataaa ttggcattag
    15361 ccaagggtaa agtgcggtca tttttcctga aatttttgta aataacgagg gcaattatac
    15421 acattttcaa actggcttga caaaatagca aaaagcaaac gtttactttt aataaacaag
    15481 aaataaatag agaaaaatgt gagataaatg acattttttg ttcagtttta tgctaaaatc
    15541 cacgccccat ttatttttac ttgacaaagg aaaatttatt atgaaactcg aagcaagcaa
    15601 gcaagcaagc agaagtttaa aaaatcattt attgtaagtc tattttttct attctttata
    15661 cctctccgct tttggctgtt gattacgttt acgacaattc taaattaaca aatgagcaaa
    15721 ttgatcgctt aaaaaaactt agagataaaa gcagcgaata ctggaaacaa gaaacttatc
    15781 ttctaaaacc gacgaatggt agctctcaga actctctcaa agttcccgca ctatactcta
    15841 aaacacacca attcaaaaac atcaacaact caaaaaagat ttctttttat gacaaggaat
    15901 acactgaaga ttaccttgtt ggcttcgccc gaggtttcgg ggttgaaaaa agaaatgggg
    15961 aagaagaaaa aacactccag caatatttta aggactgtgt aaatactggg aattccaata
    16021 atgataagtg tgaacaacca tccttcggaa actctggccc aagattaata aagagcgata
    16081 ttttcgcctt agctccacaa ataaaaaata gccatatcaa cagcgaaatt ttatctgtag
    16141 gtaattatat cgaatggctt cgcccaacac ttaataagct tgctggttgg gaagagcatc
    16201 tatattctgg tttagatcca ttccactata ttgaggttac agacaactcg cacgttatcg
    16261 ggcaaacgat tagtttggat gaattccact tagaaaattc tctctgggaa ccccgttggg
    16321 attctgatgt tggggagtta aaaacaacga atgcagacat ccgttttaat actaaaagtg
    16381 aaagtttact cgtaaaagaa gattatgctg gcggagctcg ttttcgtttt gcttacgatc
    16441 cgaaagaagc gacaaatgcc acattaattt ttgagaaaaa tataactggc acttccgaca
    16501 tcatcttcga aaatccaata gacgatttaa aaagtctcga cgggcatcaa attatcaaag
    16561 taaatggcac agcagataaa cacgcattcc gtctttctgg caaacaccaa aagggaattt
    16621 atacgctttc tttacaacaa cgcccagaag gcttttttac caaagtgcaa gaacgcgatg
    16681 atatttcgat ttatgcacaa caggctcaag ctgccaatac cttattcgcc ttgcgtttga
    16741 acgacaaaaa cagcgatatt ttcgaccgca ctttaccacg caaaggcttg tggttgcgtg
    16801 tgattgacgg acattccaac caatgggtgc aaggcaaaac agcacctgta gaaagcaatc
    16861 gtaaaggtgt gcaactcggc ggcgaagtat tcacatggca aaacgaaagt aatcaacttt
    16921 ctattggctt aatgggcgga caggcagaac aacgcagtac tttccgcaat ccagatacag
    16981 acaatcttac aacgggaaat gtgaaaggcc ttggtgcagg cgtttacgcc acttggcacc
    17041 aacttcagga caaacagaca ggtgcgtatg cggatagctg gatgcaatat caacgcttcc
    17101 gccaccgtat caacactgaa gatggtacag aacgttttac ttcaaaaggt attactgcct
    17161 caattgaagc aggttacaat gcgttattgg cggaacactt cactaaaaag gcaacagcct
    17221 tcgtgtttac ctacaaccac aggcacaatt gacttatttg ggggtaaacg gaaaattcag
    17281 cgatagcgaa aatgcccacg tgaatttact tggctcccgc caattacaaa ctcgggttgg
    17341 cgttcaagct aaagctcaat tttcactcta taaaaatatc gctattgaac ctttcgccgc
    17401 tgtgaacgcg ctttaccaca acaaaccttt cggtgtggaa atggacggag agcgtcgagt
    17461 gataaacaat aagacggcaa ttgagagcca attaggtgta gcagtgaaaa tcaaatctca
    17521 cttaacctta caagccacat tcaaccgcca aacaggcaaa catcatcaag ctaaacaagg
    17581 tgcgttgaat ttacagtgga cgttttaagt taagttgtaa caaaataaaa tcggcaggga
    17641 aattcactgc cgattttttt attttaaaag tgcggtcatt tttcgtgtaa tttccaccaa
    17701 tttttaaccg cactcacgag ctcgcgctaa accgacaatc ggatctattg tccctttctt
    17761 tgttgtgtat gcaaatcaga aatgccggtt acggaagcag tttgcccgga ttcataatgt
    17821 tatatggatc aaggtgttgt ttgatgcttt tcatcagggc gatttcggca ggcgtgcgta
    17881 ctttgtccag ccactgtttt ttgatgatac ctatgccgtg ttcggcggca atcgtgccgt
    17941 tgcaggcaag gacgttgcga tagactgtgc tgttgatgtc gttttcgtaa cgatagactt
    18001 cattgctgag gatttcgggc aggaaagtat tgtagtgcag gctgccgtcg cccagatgtc
    18061 cgaagcagac gatttgtatg cctttgaaat tctgttccaa atctttggcg caccggcgga
    18121 caaagtcggc aacgcgcccg ataggaacgg caatatcgtg tttgatgctg gtgcccagtt
    18181 tgcgttgcga tgcggagatg ttttcgcgca acgcccacat atggatacgt tcttgttcgc
    18241 tttgcgccaa cacgctgtcg gtaaagcctt ttttataaag aaattcgaca agccgatcat
    18301 caagattgct gtcgggtaat gagtcggtca actcaagtaa aatatgccat tctgaatgtg
    18361 tcggcagggg gagtttgctg aattcggaag acaattcggc ggcaaaacgg ccgatcagct
    18421 caaaactgca taggcgttcg gcaaagtgtg cttgggtttc ggtcagcagg cggacggcgg
    18481 attcgatgtc gggtatgccg acccatgcgg ttgctttgtc taaggggttg gcaaacagct
    18541 tgagcgtggc ggcagtgata atgcccaatg taccttcgct accgataaac agatggcgca
    18601 ggtcgtagcc ggtggtgttt ttatgcaggg gatggagatg ggaaaccagt tcgccgttgg
    18661 ggaggacgac ttccaaaccg ataaccaggt cgcgcatcgt gccgtaacgc aatacgttca
    18721 aacctccggc attgcaggcg atgttgccgc cgatttggca cgagccttcg ctggcgagac
    18781 tgagtgggaa cagcctgttt gaggcttcgg ctgcctgttg gacggtttgg agtacggaac
    18841 ctgcttcgac ggttatgcag ttgtctgaca aattgatgct gcggatgcgg ttgagtttgg
    18901 aaaggttcag caatacgccg ttttccgata ctgccgcgcc gcacaaacca gtattgccgc
    18961 cttgcggcgt aaccggaata cggtgttggt ggcagaaacg cataatggtt tgcacgcttt
    19021 taacgctgag cggctgcaaa atgatgtctg gtgcagacgt aaagcggttg cgctggtctt
    19081 ttaaaagcgt cggagaaggt tcggcaattt catctgccgg taggagtcgg gaaaattcgg
    19141 tatgaaggtc aagcatgttt gtcggaaggg tagggagaag attgaggaag ggaatctgat
    19201 gccgtctgaa aaatcaaaaa agcaggatac tgaacgtatc ctgctttttt gcaagcggaa
    19261 aatgcttatt ttgcagcttc ggcagcagga gcttcagcgg caggtgcttc ggtagcggca
    19321 gcttcagcgg caggtgcttc ggtagcggca gcttcagcgg caggtgcttc ggtagcggca
    19381 gcttcagcag caggagcttc ggcggcaggt gcttcagcag cgggagcttc ggcggcaggt
    19441 gcttcagcag cgggagcttc agcggctttt tcaccgccgc aggctgccag aaccaaagac
    19501 aacaaagcag cggcaaacag ggattttttc attttagtaa cctttagaac caatttaaat
    19561 tattgaaaca aataggtctt tatcgaccat actacaaacc gggttggaaa ttcatcgctg
    19621 aatttgcgta attatgcact atggaataag ggattgcaat gtcgtacaga tgggaaaaag
    19681 gtttgaatga aaagttttct ggcgggcgga ttgataaatc ctgtaaaacg gttttgctgt
    19741 taaaaattcg atagggataa atattataga gattaatata atttcagtat cttgtatgat
    19801 ttattaaggc ggattgggaa agtcggcaga caggtcggaa tggttttaag aatgttgtaa
    19861 aaaagttaca ccgcaataaa ataacaaaga tttacattga aatatgtgct gtacagttta
    19921 tgaacaggtt aacggtaatc ggccggcgcg gcggttgtgt tagaatgcgc ctgtaattaa
    19981 tctgctgttc ctgaaaggac aaatatggct atcgtgaaaa attccgtggt ttcgctgcat
    20041 tatgagatgt atgatgccaa caatcagctt ttggacaaaa ccgaagaacc gattgcgtat
    20101 ctgcacggcg gttacgacgg cattttccct ttggtggaag aggcgttgca cggtaaggat
    20161 gccggcgata cggtcgatgt ggcgctgtcg cccgacgatg cgttcggcga gcaggatccg
    20221 gagttggtcc gtatcgaaga tgcgggcgtg ttccctgttg aagtcgaagt cggcatgatg
    20281 tttgaagccg acgatcctga aaccggcgat gttgtcgtct atcgtgtaac cgatgttgcc
    20341 gacggcaagg cggtggtgga cggcaaccat cctttggcag gcatgaaaat ccgctttaag
    20401 gctacagttg aaagcgtgcg cgatgcatcc gatgaggaaa tcgcacacgg tcatgtccac
    20461 ggcccgcacg gtcatcacca ccactgattg ccggcagctt ggaggtatct gatgccgtct
    20521 gaaaagggtt tgttccgttt cagacggcat tttgtttgat ggatggagaa ggtatgtcgg
    20581 ttgggcatta tgagaatttt cccgtcggtt cgctgatttt gccgcgcagg ttgaggaagc
    20641 cggttcacgc ggtgtacgcg tttgcacgga cggcggacga tatggcggac gagggcagta
    20701 tgccgtctga agccaggttg gcggggttgg agggtttgca gcgcgagttg gacgtgttgg
    20761 catcgggcgg ccggtcggcg catcctttga ttgcacgatt ggatgccgag gcggttgtgc
    20821 cgttcggttt ggatttgcag ccgttttatg atttgctttc tgcattttct caggatgtgg
    20881 ttaaaacaag gtatggaaat ttcggcgagc tggccgatta ttgtcggcgt tccgccaacc
    20941 ctgtcggacg cattatgctg gctttgtacg ggaaaacgga tgcggtgtgc gtggcgcaaa
    21001 gcgacggcat ctgtacggct ttgcaactgg tgaatttttg gcaggatgtg gctgtggatt
    21061 ggcaaaaagg cagggtttat attccgcagg acgatttgtt gaaattcggt gtttctgagg
    21121 aacagatcgc ggcaggacgg gcggatgcgg cgtttcagcg gctgatggcg tatgagtgtc
    21181 ggcgtgcgtt ccgtatgctg aaggcgggtt cgcctttggc aagggaactg aatgggcgta
    21241 tcggtttgga actccgtatg attgtgttgg gggcgcagtt gattttgcag aaactggacg
    21301 cgtgccgata cgatgtgttt gcacaacgcc ccgttttgga taagaaggat tggttgatta
    21361 tgctgaaaaa ggcgttgtgg aaatgattgg gggaaaatga atgccgtctg aaaggggttg
    21421 ttccgcttca gacggcattt tgcaggcggt tggtatggtc aggcgatttc atgcgggttc
    21481 aatctggctt gatcttctgc acccaagccg atgagtttgc gcagtcgggt catgtagaaa
    21541 ttgacatcca aaccggtgcc gtagcgttgc gccgtccaca gtgtttccgc cagtgcctcc
    21601 atcatttcgt gttcggcttc cagccagccg cgtttggcgc acagggtgtc gtggattgcg
    21661 cgtatgccgt gcggctggtc tatgcccgcc tgttcttgga cggacagatg cagcgacata
    21721 tgcaggaagg ggttgctttc gccgttttcg ggcagccagt cggtgtccag atggtcttcg
    21781 atgcgttcga gatagcggtg gtattcggga tgggcttcga caatgcggag ggctttctgt
    21841 tccagtgcgc tcagttgcag cggattgagc cgctgctgcc acacgcgggc gaaaaagcgg
    21901 cggacatcgt gggtattgac atcgtacatg gcgggttcga taagatggtt atggaaatag
    21961 gttcggataa tggcgggttt tacataaatt aaatcccacc gtcaacctta ccatttataa
    22021 tgcagacaaa ttatttgtct gaaacggatt cgattatgaa aaaactgatt tgcctgttcg
    22081 ccgtattttt gatgttgtgc ggacgagctt tcgcgctgga tgcgaacgat ctgctgccgc
    22141 cggaaaaggc attcgtgccg gagcttgccg ttgccgacga cggtgtgaac gtccgtttca
    22201 agattgccga cggatactat atgtatcagg cgaaaatcgt cggcaagacc gatccggcgg
    22261 atttgttggg acagccttct ttcagcaagg gcgaagagaa ggaagacgag tttttcggca
    22321 ggcagacggt ttaccatcac gaagcgcagg ttgcctttcc ttatgcaaag gctgtcggcg
    22381 aaccgtataa attggttttg acctatcagg gctgtgccga agccggcgtg tgctatccgc
    22441 ccgtggatac cgagtttgat atttccggca acggcactta ccatccgcaa accgacgaac
    22501 cggcatccgc caaagaccgc tttttgcagc cttcctctca aaacggcagc ggggcgttgc
    22561 cgcccccgaa gggggatgag ggcggcgaca gccgtttcaa gctgtcttgg gatacgctca
    22621 acgccaatct tttggcgttt tttctcgccg gtttgggtct gagttttacc gcctgtatgt
    22681 atcccctgtt gccgattgtt tccagtattg tggtcggcga caaaaaggcg ggcaaggcgc
    22741 gggcgtttgt gctgtccgtc gtttatgttc agggtttggc tctgacttat acgctggtcg
    22801 gcattgttgc cggactgacg ggcgcgctgc tgaccgtatg gttgcagcag gcttgggtgg
    22861 tgttggcggc atcggcttta atggtcgtct tggcactgtc tatgttcggg ctgttcaaca
    22921 tccagcttcc caacgccgtg cagtcgtatt tccaaaacca gagcagcagg ctttcaggcg
    22981 gtaaaatcgt ttccgtcttt attatgggca tattgtccgc gctgattgtc gggccgtgcg
    23041 tcgccccgcc gctggcattt gctttgggct acatcggtca gacgggcgat gcggttttag
    23101 gcggtttggc actttacact ttggcgttgg gcaccggcgt tccgctgatt gccatcggca
    23161 cgttcggcgg gcatatcctg cctagggcag gcgattggat gaatgccgtc aaatacgctt
    23221 tcggctttat cctgcttgcc gtcgccgttt acctcgccac gccgcacttg ccctattatc
    23281 tcgtcgtcgc gctgtacacg ctgctgatgc tggttcctgc ctgtatgctg ctggtcaacg
    23341 gacgcaggca gaaacgccgt ccgaaagctg tggcattcgc attgggcggc atattgctga
    23401 taggcggcgc gtggttcggc tggcagggcg caaacggcaa aacgaccgcg ctgcaccatt
    23461 tcctgaccct caatccacca gccgaagcag gcaaatcttc ggaacacggc aaaatgtttg
    23521 ccgatactgc cgcgctgaag gcagcgatgg atacggcgtt gaaagaacat cccgacaaac
    23581 ccgtcgtttt ggatttttat gccgactggt gtatttcctg caaagaaatg gcggcataca
    23641 cgctcaatca gccggaagtg catcaggcgg ttgatatgga acgctttttc caaatcgacg
    23701 taaccgccaa cacgcctgaa catcaggcgt tgttgaaaga atacggtctg ttcggaccgc
    23761 cgggcgtatt tgtcgtccgc tccgacggca gccgcagcga gccgctgctg ggttttgtca
    23821 aagcagacaa gtttatcgag tggtatgaac aaaaccgctg attccgatgt ttcaaatgcc
    23881 gtctgaagtt ctcagacggc atttttgtca gaaaggaaaa cggcaatatc cactatacac
    23941 acggaagcag tctaaagatg tgaaaaccgt gtgtataagt tagtttgcgt attgaaatta
    24001 aaagataaaa atacgatgat taaaaaatag gcataagaaa agataaacac agccggtgtt
    24061 tgccggtgtg tgtatctttt gtggaaaaac atcttaatgt catgtatttt atggaaaata
    24121 ataacatgat taaaaaatag tcaatcatta aggttcaggt aagcggctgc aaccgtcaat
    24181 gtctgaaaag aggcagtgct gtttaagcat ggaaagccgg ataaaaaaaa ctgcctccgt
    24241 ttccggaggc agttcgtgtg cggataaaac cttacagacc gctcaaacgg gcagtgtcgt
    24301 gggcaatcat cagctcttcg ttggtcggaa tgaccacggc aacggctttg ctgtcggcgg
    24361 tggtaatcac gccggcgttg ccgaagcggg ctttcaggtt ggcttcttgg tcgatgttca
    24421 gaccgaggaa gcccaagtag ccgatcacgc gttcgcggat gatgtcggag ttttcgccga
    24481 tgccgccggt aaagaccagt gcgtccaaac cgcctgcggc aaccgccata ctgccgatgt
    24541 atttggcaag gcggtagata aacatatcca aggccaattt cgcgccttta tgccccttgg
    24601 cggcttcttc ttcaatggta cggcagtcgt tggacaggcc ggaaatgccg agcagaccgg
    24661 attttttatt cagcatttca gtgatttggg cgatggtcat attggcgttt tcggcgagga
    24721 agccgaatac ggaaggatcg atgtcgccgc tgcgcgtacc cattaccagc ccttccagcg
    24781 gggtcaggcc catactggtg tcgcgcgatt cgccgttggc gacggcggtg atggacgcgc
    24841 cgttgcccaa gtgggcaatg accatacgca ggtctttttt gtctttgccg aggaagcgcg
    24901 cggtttcgtc ggcgacgaag cggtagctgg taccgtgcgc gccgtaacgg cgcaggccgt
    24961 atttttcata caactcctgc ggaacggcgt atttgtaggc gacttcgggc atggtttggt
    25021 ggaaggaggt atcgaatacg acgacgttgg gcaggccttt gaaaatgctt tgcgcggcac
    25081 gcaggcccaa gaggtgggcg gggttgtgca ggggggcgag cgggatgcat ttttcgatgc
    25141 cggcaatgac ttcgtcgtca acgaggatgg attcgctgta cagttcgccg ccgctgacga
    25201 cgcggtggcc gatggcgccg atgcggctgt cgaggccgtg ggctttgagt tcttccatca
    25261 gggcttcgac cgcgccggtg tggtcgggat gtgcggacag atcgactttg tgtttttcgc
    25321 cgtttacttt gaatatgatg taggcatcgg gcaggttgag tttttcggca aggcagctca
    25381 gcaggacttc gccgctgccg ttatccagga ccgcgccttt gagggacgag ctgccgcagt
    25441 tcaaaaccaa gatcaatttt tgggacattt tcttactccg gaaagtttca gacggcattg
    25501 gaatcggaca cggatactaa ccggattcgt gccgaatccg ttttgccttc ctgggcggga
    25561 aagtagtggg gccgtctgaa aaggttgata aaagaagcag gctattctag caaaaaatct
    25621 ttgcaattgc ttggcttaat cgggcgtttg cgtgaaaatg gcggaagtcg ggcattttga
    25681 cggaaaaaag ggggatatgc ggtttgccgg tgttttcggc tgggtgaggg agaatttctt
    25741 tgcattcttc ccgtccaaac tggtacattc gccctgtttt tagttggtgt cgtctgaaaa
    25801 aaggcgggtg ggaaatttat gcattatttc agtattcatg accaatcggg acggcatata
    25861 ggctttttca tcctgcttgc cgacgatgag tcggaggcgc ggccgcagag cggacgtttt
    25921 gccgttaaat tggaagatgg agcgggcaac catgtcctgt cgccgtttgc ccagacggaa
    25981 atcccccaat attggcgcgt ggtcaaagac cgtatcgagc tgttttttga cgaagccccc
    26041 gtcggaactt tgcgtaacga atatctgacc gtatcggggc agacttttgt tttgaacgat
    26101 ttgacaggga atatatgatg gaaagtatgt ttatattggt gcccatcagc attattttgg
    26161 cgtttgtcat cggctggttt ttttggtggt cgggcaaaaa cgggcagttt gacgacttgg
    26221 aagggccggc gcaccgcatc ctgatggatg atgattcgac aagtaaactg attgaaaaag
    26281 agaaggaaaa cgatgtacgc taaaaaaggc ggtttgggac tggttaaaag ccgccgtttc
    26341 gcacctcttt tcgctacgca gtttctcggc gcgttcaacg acaatgtgtt caaaaccgcg
    26401 ctgtttgtga tgattgggtt ttacggtttg gggcaaaacg gcttcctgcc tgccggacag
    26461 atgttgaact tgggcgcgtt gttgtttatt ttgccgtatt tcctgttttc ctcgctgtcg
    26521 gggcagttgg gtaacaaatt cgacaaggcc gttttggcgc gttgggtcaa ggtgctggaa
    26581 atgatcatta tggcggtggc ggcatacggg ttttatatcc ggtctgcccc gctgcttttg
    26641 gcgtgtctgt tttgcatggg cgcgcaatcg acgctgttcg ggccgctgaa atacgccatc
    26701 ctgcccgatt atctcgacga caaagagttg atgatgggca acagcctgat tgaatcgggt
    26761 acgtttgtcg ccatcctgtt cggtcagatt ttgggaacgg cggtggcagg tgtaccgcct
    26821 tatattgtcg ggatactggt tttgctggtc gccgtaggag gcacggtcgg cagcctgttt
    26881 atgccgtccg tacccgccaa ggctgccgat acacaaattg agtggaatat tgtccggggt
    26941 acaaaatccc tgctgcgtga aacggtgcgg cacaagcccg tttttaccgc cattatcggt
    27001 atttcgtggt tttggtttgt cggcgcgatt tataccacgc aactgccgac ctttacccaa
    27061 atccatctag gcggcaacga caatgttttc aacctgatgc ttgccctgtt ttccatcggt
    27121 attgccgccg gttcggtact gtgtgccaag ttcagcaggg aacggctgat gttggcttgg
    27181 gtaacggttg gtgcgttggg tttgacggtt tgcggtctgg ttttggtgtg gctgacgcac
    27241 ggacaccgtt ttgaagggct gaacggcatt ttttggtttt tatcgcaagg atgggcatac
    27301 cccgtgatgg cggtgatgac gctgatcggc tttttcggcg gatttttctc cgttccgctc
    27361 tatacctggc tgcaaaccgc cagcagcgag actttccgcg cccgcgccgt tgccgccaac
    27421 aatatcgtta acggtatttt tatggtttcc gccgccgttt tgagcgcggt gttgctgttt
    27481 ttgtttgaca gcatttcctt gttgtatctg attgtcgcct tgggcaatat tccgttgtcg
    27541 gtatttttga ttaagcgcga aaggcggttt ttaggcgcgg cggcaatcag gaaagaacct
    27601 tgaatgccgt ctgaaaagtt tgtccccgca atacacaata cgcctgccca atgttgtgaa
    27661 cacactgtga ataagtgggt ttgtgcattg aaatgaaaac agaaaaatac gctgcctaaa
    27721 aaatagtcag cagatggaaa atttgaagaa agaagcagac atgattcaac gccaaatcat
    27781 cctcgatacc gaaaccaccg gcctgtatgc cgatggcggc gaccgcttgg ttgagtttgc
    27841 cggtctggaa atggtcaacc gccaaatgac cgacaaaaac ctgcaccttt atgtccaccc
    27901 cgagcgcgat atgcctgaag aggcggcgag ggtgcacggt ctgacgatag aggttttgga
    27961 agggaaaaac gcaccgcctt ttgcagaggt cggcaggcag attgccgatt ttctgcgcgg
    28021 tgcggagttg attatccaca atgccaaatt cgatgtgggc ttcctcaata tggagttccg
    28081 ccggatgggg ctgccgactg tcgaggaact cggctgtacc gttaccgata ccctggcgat
    28141 ggcgcgcgaa atgttccccg ggcagaaagc cagcctagat gccctgtgca accgtttttc
    28201 cgtcgaccgc agcaagcgcg tcctgcacgg cgcattgatc gactgcgaac ttttgggcga
    28261 agtctatctt gctatgacgc ggcggcagtt cgacctgatg ggggaggccg cagaggaaaa
    28321 aatggaaacc aaacccgtgg tgcataccga aaccaaacgc agcggtaaac tgaaagtcat
    28381 cagggcggac gaaaacgagt tggcggcgca cgggcaatat ttggacggct tgggcgaagc
    28441 ctgtatctgg cgcaaggaag ccgtgccgtc tgaaaacggc gggacggacg catgaagcgc
    28501 aaaaatatcg cgctgattcc cgccgccggc atcggggcgc gtttcggtgc ggacaaaccc
    28561 aagcaatatg tcgaaatcgg aagcaaaacc gttttagaac atacgattgg gatttttgaa
    28621 cggcatgagg gcgtcgattt gaccgtcgtt gtcgtctcgc ccgaagacac gtttgccgat
    28681 aaggttcaga cggcatttcc acaggttcgg gtgtggaaaa acggcggaca gacccgcgcc
    28741 gaaaccgtcc gaaacggtgt ggcaaaactg ttggaaatcg gcttggcatc ggcggatgac
    28801 aatattctgg tacacgatgc cgcgcgctgc tgcctgccgt ctgaagcttt gacgcggttg
    28861 atagaacagg cgggcaacgc tgccgaaggt gggattttgg caattcccgt tgccgatacg
    28921 ctcaagcgcg cggacggtgg gaacattagt gcaaccgtcg agcggacgag cctttggcag
    28981 gcgcaaacgc cgcagctttt ccgcgccggg ctgctgcacc gcgcattggc tgcggaaaac
    29041 ttggacggca ttaccgatga agcgtccgcc gtggaaaaat tgggtgtgcg tccgctactg
    29101 atacagggcg acgtgcgcaa tttgaaactg acgcagccgc aggacgcata catcgtcagg
    29161 ctgctgctcg atgccgtctg aacttcaatt caaaacatca ggaacacaaa cgatgacgaa
    29221 catccgtatc ggacagggct acgatgtcca ccaactgacc gaaggcagaa agctgatact
    29281 tggcggagtt gaaatcccat ttgaaaaagg gctgctcgga cactccgatg ccgatgcgct
    29341 gctgcacgcc gttaccgacg cgctgctcgg tgcggcaggt ttgggcgata tcggcagcca
    29401 tttccccgac accgccgcag agttcaaaga tgcggacagc cgcgtccttt tgcgtgcggc
    29461 gtatcaaagc gttcaggcgc agggttggca ggcggtcaat gtcgatacga ccgtgattgc
    29521 acagaaaccc aaactcgcgc cgcatattcc gcaaatgcgt gccaacatcg ccgccgattt
    29581 gggcatagac atttcgtgcg tcaacatcaa aggcaaaacc aacgaaaaac tcggttatct
    29641 cgggcgcatg gaaggcatag aggcgcaggc ggcggtattg ctggtacgga tttgaacgcg
    29701 gcgggcagga cgtgcaaaaa aatgttaaga tgacggctgt ttgttgatac ggtttcccaa
    29761 aggacaaaaa tatgacgaca caagacgaac tcaagcgcat cgccgccgaa aaagcagtcg
    29821 aattcgtacc cgaaaacgaa tacatcggca tcggtaccgg ttcgaccatc aactttttca
    29881 tcgaagcctt gggtaagagc ggcaaaaaaa tcaaaggcgc ggtatctact tccaaaaaat
    29941 ccagcgaact gcttgcccaa tatgatattc ccgtcgtttc cctgaacgaa gtatcggggt
    30001 tggcggtcta tattgacggt gcggatgaag tgaaccacgc cctgcaaatg attaaaggcg
    30061 gcggcggcgc acacctcaac gaaaaaatcg tcgccagcgc atcagaaaaa ttcgtctgca
    30121 ttgcggatga aagcaaatat gtttcacgtt tgggcaaatt ccctctgcct gtggaagtcg
    30181 tcgaaagcgc gcgttccctt gtttcgcgca aactgctcgc tatgggcgga cagcctgaat
    30241 tgcgtatcgg ctacaccact ttttacggca accaaatcgt cgatgtccac ggcttgaata
    30301 tcgacaaacc gctgacgatg gaagacgaaa tcaacaaaat caccggcgta ctcgaaaacg
    30361 gaatattcgc ccgcgatgcc gccgatgtgt tgattttggg gacggaagag ggtgcgaaag
    30421 tcatctatcc ctgccaaggg taatcctgac gttaagatgc cgtctgaaat ccgattccga
    30481 tatgttcaga cggcatcggc atttgaaaaa tatgcagatt aaaaaaatca tgaaatggtt
    30541 tcccgtcgcc ctgtcgcttt tgggtgcgtt gggttatacg gggtacggca gcgaggcggt
    30601 gcggacggcg gttgccgtac tcgacgtact cggcgcggca ggggacgcgg gttccgacgc
    30661 gcccgcccgc cgacgggcat cggcgaaatc cggccaccgc tacacaggca cggtgtccaa
    30721 agtctatgac ggcgacaccc ttcacgttat cgacggcgac ggcgcgaaac acaaaatccg
    30781 gatggcgtat atcgacgcgc cggagatgaa acaggcttac ggcacgcgtt cgcgcgacaa
    30841 cctgcgcgcg gcggcggaag gcaggaaagt cagcgtgcgc gtgttcgata ccgaccgcta
    30901 ccagcgcgaa gtggcgcagg tttctgtcgg caaaaccgat ttgaacctga tgcaggtgca
    30961 ggacggggcg gcgtggcatt ataaaagtta tgctaaagaa cagcaggata aggcggattt
    31021 tgccgactat gccgacgctc aaattcaggc ggaaagggaa cgcaaaggat tgtggaaagc
    31081 taaaaatccg caagcgccgt gggcgtaccg ccgagcaggc aggagcggcg ggggcaataa
    31141 ggattggatg gatgccgtgg gcgaatggtt gggcatttgg taaagacagc gcacggcttt
    31201 gaacggcaaa tgccgtctga aaaccgcgcg ccctgcattt ttgcggagac gcgcaaccgc
    31261 cgtctgccgc gataaaaaag ttcaggcggc ggtttttacg gtaaaatggc ggattatttt
    31321 gcaacggcgg cagttccgcc ggtttttgat acggacggta tatggatttt cgttttgaca
    31381 ttatttacga ataccgctgg atgttccttt acggcgcact gacgaccttg gggctgacgg
    31441 tcgtggcgac ggcgggcggt tcggtattgg gtctgttgtt ggcgttggcg cgcctgattc
    31501 acttggaaaa agccggtgcg ccgatgcgcg tgctggcgtg ggcgttgcgt aaagtttcgc
    31561 tgctgtatgt tacgctgttc cggggtacgc cgctgtttgt gcagattgtg atttgggcgt
    31621 atgtgtggtt tccgtttttc gtccatcctt cagacggcat tttggtcagc ggcgaggcgg
    31681 caatcgcgct gcgtcgcgga tacgggccgc tgattgccgg ttctttggca ctgatcgcca
    31741 actcgggggc gtatatctgt gagattttcc gcgcgggcat ccagtctata gacaaaggac
    31801 agatggaggc ggcgcgttct ttggggctga cctatccgca ggcgatgcgc tatgtgattc
    31861 tgccgcaggc attgcgccgc atgctgccgc ctttggcgag cgagttcatc acgctcttga
    31921 aagacagctc gctgctgtcg gtcattgctg tggcggagtt ggcgtatgtt cagaatacga
    31981 ttacgggccg gtattcggtt tatgaagaac cgctttacac cgtcgccctg atttatctgt
    32041 tgatgacgac tttcttaggc tggatattcc tgcgtttgga aaaacgttac aatccgcaac
    32101 accgctgatt ggagtcatat caacaaaaag ctgcccgggt attcatttga ggcagctttt
    32161 ttgtggcaaa tttat
//
LOCUS       DAXHNE010000006        31894 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.006, whole genome shotgun sequence.
ACCESSION   DAXHNE010000006 DAXHNE010000000
VERSION     DAXHNE010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31894
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.006"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(178..825)
                     /locus_tag="ACFM4Z_000203"
     CDS             complement(178..825)
                     /locus_tag="ACFM4Z_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HFC6020310.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            complement(822..1190)
                     /locus_tag="ACFM4Z_000204"
     CDS             complement(822..1190)
                     /locus_tag="ACFM4Z_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HFC6020311.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            complement(1200..1871)
                     /locus_tag="ACFM4Z_000205"
     CDS             complement(1200..1871)
                     /locus_tag="ACFM4Z_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HFC6020312.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVEKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIIYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            complement(2036..2755)
                     /locus_tag="ACFM4Z_000206"
     CDS             complement(2036..2755)
                     /locus_tag="ACFM4Z_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6020313.1"
                     /translation="MATLSDKTILITGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGHPEPFAIRFDLMSAEEKEFNQFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            complement(2839..4152)
                     /gene="hpnE"
                     /locus_tag="ACFM4Z_000207"
     CDS             complement(2839..4152)
                     /gene="hpnE"
                     /locus_tag="ACFM4Z_000207"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HFC6020314.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLTRHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRVPSAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNTIPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTAQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFTNRAWADKVHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFAAAETCLQSLSDAV"
     gene            complement(4182..4388)
                     /locus_tag="ACFM4Z_000208"
     CDS             complement(4182..4388)
                     /locus_tag="ACFM4Z_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020315.1"
                     /translation="MKAVRDIALWLAVTVWINFFPDNYRYDTVPQGRYGYWHADHPWY
                     PYARFLLPLFLACILFYRHFRKRK"
     gene            complement(4550..5422)
                     /gene="hpnD"
                     /locus_tag="ACFM4Z_000209"
     CDS             complement(4550..5422)
                     /gene="hpnD"
                     /locus_tag="ACFM4Z_000209"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HFC6020316.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTILYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCHRVAGVVGCLIARILGFSDGKTLQYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPAADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            5593..7455
                     /gene="hscA"
                     /locus_tag="ACFM4Z_000210"
     CDS             5593..7455
                     /gene="hscA"
                     /locus_tag="ACFM4Z_000210"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFC6020317.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKIALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCYLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVKQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKADGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVA
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVHAQIEVKPSYGL
                     DDGAITRMLKDSMDNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEELQQI
                     RQGIADLQGRLKDGKAEDIRAAAAKLGSITDNFAAKRMNRNIQRALTGQSVDNI"
     gene            7532..8188
                     /locus_tag="ACFM4Z_000211"
     CDS             7532..8188
                     /locus_tag="ACFM4Z_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6020318.1"
                     /translation="MEKSGIVYLMKTVIKGVYKIGISEVSNFEGRMRHLENNGYANVA
                     GLERILAVKTDNYKEKENLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKN
                     ETAESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPRKSK
                     IRLFKEAYFGKSGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPR
                     "
     gene            8224..8985
                     /locus_tag="ACFM4Z_000212"
     CDS             8224..8985
                     /locus_tag="ACFM4Z_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HFC6020319.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNKPGELTNIEQGESICV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLTGWQNVADLLEPRH"
     gene            9040..9381
                     /gene="fdx"
                     /locus_tag="ACFM4Z_000213"
     CDS             9040..9381
                     /gene="fdx"
                     /locus_tag="ACFM4Z_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFC6020320.1"
                     /translation="MPKITVLPHTTLCPEGAVIDDAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(9804..10358)
                     /locus_tag="ACFM4Z_000214"
     CDS             complement(9804..10358)
                     /locus_tag="ACFM4Z_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFC6020321.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHHQNRRDLIALDKAAAGCDSAMLRAQRWLIYTQIAERLAGSGGGFT
                     VTVESVSAACPELEGRYLELVRRAAVSFGFTQGWENPAGGQEKTQAVPDAASGHSVRK
                     PIKINIEARVERYPAYCPTVALKV"
     gene            10571..10768
                     /gene="iscX"
                     /locus_tag="ACFM4Z_000215"
     CDS             10571..10768
                     /gene="iscX"
                     /locus_tag="ACFM4Z_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFC6020322.1"
                     /translation="MKWTDTQRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(10836..11237)
                     /locus_tag="ACFM4Z_000216"
     CDS             complement(10836..11237)
                     /locus_tag="ACFM4Z_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC6020323.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTATLREERKQL
                     DQFSRITSAYPDGRPTKRDRRQLDRLKKGDW"
     gene            11449..12408
                     /locus_tag="ACFM4Z_000217"
     CDS             11449..12408
                     /locus_tag="ACFM4Z_000217"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFC6020324.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDYAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            12476..13786
                     /gene="tilS"
                     /locus_tag="ACFM4Z_000218"
     CDS             12476..13786
                     /gene="tilS"
                     /locus_tag="ACFM4Z_000218"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFC6020325.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNTDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLDEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFRLEKTDKLRFLKDRQISGNLR
                     EILTGQGFVLKRHPFGLPEHLLEQDGILRTVAASDTLAMGGIHKDVKKILQGKRVLPV
                     LRPIWPLVADSGNRPLALANCCADFQYSVSDGILPVHPDFPILF"
     gene            13860..14645
                     /locus_tag="ACFM4Z_000219"
     CDS             13860..14645
                     /locus_tag="ACFM4Z_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFC6020326.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGMPVGVYIPEAKMPSEEVRKLTAVLPEDGFFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGIGAVILGKGCADAWSPKVLRAGM
                     GAHFLSEIYPQADLEIWLVRYKGRVFATALREEKQAVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRAAY"
     gene            14655..14795
                     /locus_tag="ACFM4Z_000220"
     CDS             14655..14795
                     /locus_tag="ACFM4Z_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020327.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LI"
     gene            14780..14932
                     /locus_tag="ACFM4Z_000221"
     CDS             14780..14932
                     /locus_tag="ACFM4Z_000221"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020328.1"
                     /translation="METHLIVQCRGGGGFGLGVVEMVLTDIAQNRANGIRTNKEFGKK
                     FVKSTN"
     gene            15114..15466
                     /locus_tag="ACFM4Z_000222"
                     /pseudo
     CDS             15114..15466
                     /locus_tag="ACFM4Z_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691158.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15478..15783
                     /locus_tag="ACFM4Z_000223"
     CDS             15478..15783
                     /locus_tag="ACFM4Z_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020329.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPKGNAQKDGSAAGIKPH"
     gene            15845..17221
                     /gene="mpl"
                     /locus_tag="ACFM4Z_000224"
     CDS             15845..17221
                     /gene="mpl"
                     /locus_tag="ACFM4Z_000224"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFC6020330.1"
                     /translation="MKHIHIIGIGGTFMGGLAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIDVYEGFDAAQLDEFKADVYVIGNVAKRGMDVVEAILNLGLPYISGPQWLSENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFGVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHYL
                     VRTVPSEGLIVCNGRQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     TAGRVKWDLMGRHNRMNALAVIAAARHVGVDIQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKSALPVSLKEADQ
                     VFCYAGGVDWDVAEALAPLGGRLNVGKDFDAFVAEIVKNAEVGDHILVMSNGGFGGIH
                     GKLLEALR"
     gene            17428..18480
                     /gene="bioB"
                     /locus_tag="ACFM4Z_000225"
     CDS             17428..18480
                     /gene="bioB"
                     /locus_tag="ACFM4Z_000225"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFC6020331.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRQHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKAFGIKVDY"
     gene            complement(18558..19193)
                     /locus_tag="ACFM4Z_000226"
     CDS             complement(18558..19193)
                     /locus_tag="ACFM4Z_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC6020332.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     RKRITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGPHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGRFVS
                     GNIIPITQVGDKIPKIDPEKTVIGRIIYLNRSDFPKGNGLDVSPDGSITRR"
     gene            complement(19317..19556)
                     /locus_tag="ACFM4Z_000227"
     CDS             complement(19317..19556)
                     /locus_tag="ACFM4Z_000227"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020333.1"
                     /translation="MGVCTFFRRTAFVKQRAFEVADGNVRVLQGRFDVFQNIGIGQVI
                     DRIIAAEHNVAHADNGNGIGCLFVRRILSVHIAGI"
     gene            19543..19776
                     /locus_tag="ACFM4Z_000228"
     CDS             19543..19776
                     /locus_tag="ACFM4Z_000228"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC6020334.1"
                     /translation="MQTPKICYAFRTPSAYGQYLADAGFDYLSFANNHSNDFGAQGIT
                     ATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(19839..21698)
                     /gene="ilvD"
                     /locus_tag="ACFM4Z_000229"
     CDS             complement(19839..21698)
                     /gene="ilvD"
                     /locus_tag="ACFM4Z_000229"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFC6020335.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEQWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPKNRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(21871..23640)
                     /gene="cysI"
                     /locus_tag="ACFM4Z_000230"
     CDS             complement(21871..23640)
                     /gene="cysI"
                     /locus_tag="ACFM4Z_000230"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFC6020336.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAVSDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPVRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEEFARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            complement(23667..25481)
                     /locus_tag="ACFM4Z_000231"
     CDS             complement(23667..25481)
                     /locus_tag="ACFM4Z_000231"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HFC6020337.1"
                     /translation="MSGHDMQNTNPPLPPMPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPAAEPFSVTVLSASQTGNAKSVADKAADSLKAAGIQVRRAELKD
                     YKAKNIADERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCQAGKDFDRRFEELGAKRLIERVDADLDFTASANAWTDNIAALLKEEAAKNRATP
                     APQATPPAGLQTVPEGRYSKADPFPAALLANQKITARQSDKDVRHIELDLSGSDLSYQ
                     AGDALGVWFDNNPALVGEILDLLGIDPATEIQAGGKMMPVARALSSHFELTQNTPAFV
                     KGYAAFAHYEELDKIIADNAVLQDFVQNTPIVDVLHRFPASLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARAGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDKSKPIVMIGSGTGIAPFRAFLQQRAAENATGKNWLFFGNPHFARDFLYQTEWQ
                     QFAKEGFLHKYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(25478..26764)
                     /locus_tag="ACFM4Z_000232"
     CDS             complement(25478..26764)
                     /locus_tag="ACFM4Z_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HFC6020338.1"
                     /translation="MTAQHQAPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKEAIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDVHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ENLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVEQTVRIEPNG
                     LTAKVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARRYLLKHGTQTLPAKVGEIESVLDVLTLEQEAGAESLKINDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(26803..27726)
                     /gene="cysD"
                     /locus_tag="ACFM4Z_000233"
     CDS             complement(26803..27726)
                     /gene="cysD"
                     /locus_tag="ACFM4Z_000233"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFC6020339.1"
                     /translation="MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPAEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(27764..28504)
                     /locus_tag="ACFM4Z_000234"
     CDS             complement(27764..28504)
                     /locus_tag="ACFM4Z_000234"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HFC6020340.1"
                     /translation="METTLFKPALWQIPHIGSGGETALAEKTETLKQRLHRIVGSHRD
                     ARFASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHTETLNLLDRLGRAYPHLRIKRF
                     RPVREDADRYVESKGRFAFYDSVEARRECCRIRKIEPLNRAIAGADAWLTGQRREQSA
                     TRTELPFAEYDAGRGIGKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCT
                     RPVKAGEDIRAGRWWWEGRNSKECGLHK"
     gene            complement(28515..29957)
                     /gene="cysG"
                     /locus_tag="ACFM4Z_000235"
     CDS             complement(28515..29957)
                     /gene="cysG"
                     /locus_tag="ACFM4Z_000235"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFC6020341.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLNAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPHSLGDMAEISGR
                     WRDAVKAKLKSVTERRRFWEKQFNGRFAALVKNQQTAQAEQELAKQLEQNYQGGFVSL
                     VGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGDRTP
                     QEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAAVGA
                     TAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALIAER
                     LQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLHEKL
                     AWFGENGEGENRVGQAYPALGGLNAGQRAA"
     gene            complement(30082..30240)
                     /locus_tag="ACFM4Z_000236"
     CDS             complement(30082..30240)
                     /locus_tag="ACFM4Z_000236"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020342.1"
                     /translation="MPSEALFALRTASPHHANGLNARNGFRPFFFRNRYKTENRRIRS
                     RPHIAFSF"
     gene            complement(30253..30909)
                     /locus_tag="ACFM4Z_000237"
     CDS             complement(30253..30909)
                     /locus_tag="ACFM4Z_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HFC6020343.1"
                     /translation="MKNKTKVWDLPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LFVLFLLVFRLCWGIWGSDTARFSRFVQGWAGIRGYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRVFKKKNLILPMITGFKYIEGKTSIRFAGKAALAAALSVASLAAAAILLLS
                     "
     gene            30973..31863
                     /locus_tag="ACFM4Z_000238"
     CDS             30973..31863
                     /locus_tag="ACFM4Z_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFC6020344.1"
                     /translation="MFPVFHLSGESRRQMLQTALRFPHVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDTLPFAVIAGFPEIMLDTADSLAKRQD
                     INAWVVGCGLGTGRAAVGTLAGILTEHTDKPVVLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAIFGATVVLKGHKTLVASPDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITKSM"
BASE COUNT         6880 a         8358 c         9249 g         7407 t
ORIGIN      
        1 tacaaccacg ccggaattaa gtttaaattt gaataaaagg ttcgggttct gcaaaataca
       61 gaacccgaac cttgttcgga tattgaaacc ggctgcccga ttttgggcgg tgcggcttgc
      121 aagtatcaag attcgcatat gccgtctgaa gctcggagag gttcagacgg catatgctta
      181 tttgggctgc tcttcaacga atctcggacc tttcaagatg ccgttgtgag aatagggcga
      241 cagcaggttg tatgcggcgg ttttggaaac ctgataaccg cggtcggtca ggctgttggc
      301 aatctgattg accactgcgc tgaccaaagc ccccaacagg ccgctgttgc tgttgttgct
      361 gccttcgcgg atgctggccg aacccgacca caactctttt ccgttgcggg aatcgaccag
      421 ccgtgctttg gcggatacgg tcgtcacgct gtctaaaatt tgatatgaag tgccgtattc
      481 ggtaaccgta atgtacaaaa ccgcatcatt gccgaaaatc tgatgcagtt tttccggccg
      541 gacggcgtga atatcggcgg cattggtcaa gccgttttgt ttgaaggttt cctccacgac
      601 tgcggcgggg aagacgtaat agccggcttc ggaaagcggc gcggcggtcg aagccagtac
      661 accccatgtt ccgttgacat cgggcgattc gttcagcggc ggaaccacca aaattgaagc
      721 cggtttgctt tccttgaatg acgtgtagtc gaaatcgggc gctttttgaa cttggcaggc
      781 agacagcgcc aacacggcgg caagccctaa aatcaaaggt ttcatcgctt gcctccttta
      841 cctgttttca tcaggaagtc cataaatacg cccgattcgg gaaacagcct tttctcttct
      901 tcaaactggc ggaacgcgcc ctctttgtct cccgaacggg aaagcagcag tcccagatgg
      961 gcgtgcgcac ccggggcggc attcattttt ttgttgccgg cttccacaaa gtatttttcc
     1021 atcttttcgg tctgcttgcc caacgaagtg tcgtcgtttt tcaaaccttc atagacggta
     1081 tcgggatagc cgccgtaata atacagggat ttttgcccgt tgccgccgca ggcggtcaga
     1141 gccaagaccg ccgcacacag cgacaaacgg ctcaaggttt tcggattcat catttctcct
     1201 taacggttgg gttgccatgc gccgttgtca acagcctgaa ccaggctgtt gacggcttcg
     1261 cggattgcca agtctaaaac tttgccgttc aaagtcgcat cgtagccgga agtgccgccg
     1321 aaaccgatga tttcacggtt ggaaagtgcg tattcgcccg cgccctgtgc ggaatagatg
     1381 atttcggaag tattgacgtt gacgatattc agagccactt ttgcataggc gatttgcgat
     1441 ttgccgcgac ccaaaatgcc gaagagctga tgatcgccga catctctgcg tccgaattcg
     1501 gttacatcgc cggtaacgac ataatctgcg cctttcaggt tatgcgcttt gccggaaatg
     1561 ccggattcct gttttaatgc gttcaaattg gtgcggttca gtacgttgaa gcggttggtc
     1621 tgttgcaggt gcgttaccag aatggttttt gcctggctgc ccaaacggtc ttccccgtcg
     1681 gagaaaatgc ctttttggaa gctggagcgg ttgtcgaatg ttccgacgga aatcggggta
     1741 cgaacaccgt gatattgcgt attgtaggag gcgactttct ctacctcgag actgcgtgag
     1801 gattcggtcg cacagccggt cagtgaaacg gcagcggcgg caaggacaac ggcggtggaa
     1861 acggttttca taaaatttac cctaaggtca agttaaggaa ataacgggtt atcattattg
     1921 tccttatgta aatttaagtc aaggtgtttg tctgtgcggg acggatgcgc gcggaaggta
     1981 cggatatttt tcaaacggaa tcgcggcgcg ggcgggatgc ctgccttttc cggatttaga
     2041 ggtaaacgat ttcgccactc cgccctttgc tttcggcact tgcccaccag acaaatgcgg
     2101 gcagcacgtc cccgtagctt ttgcgttcgc ttttggcttc gcccggatgg gatttgatgc
     2161 gttgcgggga attgatgggg ccggggacga ggacgttggc gcgcaggttg ccgaagcgtt
     2221 cccattcgtc ggcggcgact ttgcacaggt agttcaacgc ggctttggac gcgccgaagc
     2281 cgccccagta ggctttgggt gtttcgccgt ggctttcgcc gacgaagatg acggacgcgt
     2341 cgggcgactg cttcagcagc gggaacaggg cgcgggtcag ccccataggt gcgacggtgt
     2401 tgatgcggta ttggttgacc cattcggcga cggtttggaa atccagcggc gagagggcgt
     2461 aaaaatagcc ggcgcagtgg acgatgccgt ccagtttgcc ttgcgtggct tcggcaatgg
     2521 tggcggcgaa ctgattgaat tctttttctt ccgcactcat caggtcgaaa cggatggcga
     2581 agggttcggg gtgtccggct tcgacaatcg cgtcatagac tttttccagt tttttctgat
     2641 ggcgtgccac cagaatcacg gttgcgcctg ccgccgcata ggctttggcg acctgttcgc
     2701 ccagaccttg cgatgcgccg gtaattaaga tggttttgtc ggacagtgtc gccatacttt
     2761 ttcctttttg gtggtcggtc gggggtattt tagcgttttg ccgcaccttg taaagcgtcc
     2821 cgatgccggc cggcgttttc agacggcatc gctcaggctt tgcaggcagg tttccgccgc
     2881 cgcgaaacct gattgtacgg cggcttcgag cgtggcgggg tagtccgggt ggaggtagtc
     2941 gccggcgggg aagatgcggt gccggtgcag ccacgacaaa tccggcggcg gggcatcggc
     3001 tgcggttgtg gcgcgttttt cggtgatgac gcgcacggct tcgggttcgc ccaaatgcgg
     3061 aaggatgcgt ttgaggtcgg cgtgaacttt gtccgcccac gctcggtttg taaacgcgcc
     3121 gacgcggtcg gaaacgctga tgacggcgga cacttcgttt tcaggcagtc cgagcctgcc
     3181 ccggcaaagc agccattgtg ccgtgccgtc ggcaaggccg gtcagcgggg cgggcaggcg
     3241 gacgggttcg gcgtagcgca gatagacggt ggtgatggcg tgatagcgaa ggttttgata
     3301 tgccgtctga acgtgttcgg gcgtgccttc gggcaggagc gcggcggcgt ggtagggcgc
     3361 ggtggcgggg acggcggcat cgaaagcttc gccgttgacg agcactttcc cgtccgggat
     3421 ggtgttcaga cggcatacgc gcgtttcgag gcggatgtcc gcgccgagcc gttgaagctc
     3481 cgccaaggcg ggttcggcga cgattgcgcc caaatcctgc ttgggtagga gatagtcgct
     3541 gccggatttt ttcgtcagca cgccgtcgga caaaacgttg cacaacacgc gcaggcttgc
     3601 ggtttccaaa ggcgtgttga gcgcgcccca caccagcggc tgccaaaact gcatcacggc
     3661 ggcacgcggc acgttccgct gtttcagcca ttgtgcaact gtcgtgtcgg gctgtccgag
     3721 gcgtgcggac ttctgcaaat cggacatatc ggcaagcagt ttggctttga atgcggacgg
     3781 tacgcgccgg gcaagcagca cgccgcccaa aatatgcagc ggtgcgggca gggggagggc
     3841 gcggaactgc aaaccgccgt gcatatgcca gtgcagcggt acgcgcaaaa aggcggcacg
     3901 gggatccgaa ccgatggttt tcatcaggcg caacacgccc cggtatgcgc cgagcaaaat
     3961 gtgctgcccg ttgtccaaaa aaccgaaacc gtcggtattt ccggcaagtg tgcgcgccct
     4021 gccgcccgcc tgccggccgg cttcaaacag ggtaacgtcg gcgtgccgcg tcaaggtgac
     4081 ggcggcggac aagccggccc agcctgcgcc gatgacggcg attttcgggc gcggatgcgg
     4141 cgtgttcatc atttattcct ccaatggttt tgcagccgta tctatttccg tttccgaaaa
     4201 tgacggtaga aaaggataca ggctaaaaat aaaggcagca gaaagcgcgc atagggatac
     4261 cacggatggt ctgcatgcca gtatccgtac cgtccctgtg gaacggtatc atagcggtaa
     4321 ttgtcgggga aaaagttgat ccaaaccgtt actgccagcc ataaggctat atccctgact
     4381 gctttcattt acttctgctc ctgtttaaat tcccagcaat tcgatttcaa agcgcgaacg
     4441 ccaacgggat tgcgcggtta cgatgcagac tttcagacga tggttgaaac cccgtccggc
     4501 ggggctgtgg gaatcgggtt tgcctgacgg gcggcgggcg gtatgcgcct tatgcccgtt
     4561 ccggcgtgcc ggggcgcggt ttgaatccga ataaccaggt tttcagggca atgcgttttt
     4621 tgcgcggcga agggagggcg attttgtatt tgaggacgtt ttgcgcgccg tcccggtcga
     4681 tttcgttcaa tagctcgtaa taaaccgccg ccataaccag tccgactttt tgggcttttt
     4741 tatcggcagc cggcagcagc gatacggctt cgcggtaggt ttcacgggcg cgtttgattt
     4801 ggaacgccat caattcggca aaattgcccg tcgggctgca ttgcaaaatc acgcttgcgg
     4861 ggacatcaaa ccgctgcatt tcctccatcg gcaggtaaat ccgtcccctg cgcgcgtctt
     4921 cgccgacatc gcggatgatg ttggtcagtt gcagcgcaag tcccatcttg tcggcgtatt
     4981 gcagcgtttt gccgtctgaa aaccccaaaa tccgcgcaat caggcagccg actacgcctg
     5041 cgacgcggtg gcaatacagt ttcaattcct caaaactgcc gtaacgcgcc tgaaccaaat
     5101 ccatctgcat cccgtcgatt aaggcttcca gttcatattt cggcagcttg aaggtttcct
     5161 taacctgccg caaggcctga ttgacggggt gttccggcat cgcgccgccg aataccttgt
     5221 ccaaatcgcc gcgccaccag ttcaatgttg cctgtgcaac atcggggttg gaacattcgt
     5281 caaccacatc gtccaattcg cggcaaaagg catataaaat cgttaccgca tcccgttttt
     5341 cctgagtcag gaaacggaag cccgataaaa aactggagcg gctttcttct gctttttgac
     5401 ggcaatagtc gagtcctttc acgatttata ttcctaatga tgggcgggaa aggcggattt
     5461 tatcggcatt tggcggtaga gggcaatttc ggcggcacga cccaatcctt agcggtttgc
     5521 tcaactatcg gcgcaaattc tgttaaaatg ccgctttcct tcctttacac accgcaccga
     5581 caggcagaat ttatggctct tttgcagatt tcagaaccgg gtatgtccgc cgccccgcac
     5641 cggcaccgtt tggcggcagg catcgatttg ggtacgacca acagcttggt cgccaccgtc
     5701 cgcagcggca gtgccgcctg cctgcccgat gccgaagggc gcgttaccct gccttccgtc
     5761 gtccgctatc tggaaaacgg cggcattgaa gtcggcaaaa tcgccctgtc cgcccaaaaa
     5821 accgacccgc tgaacaccgt cagctccgcc aaacgcctta tcggacggac gcttgccgat
     5881 ctgcatcaaa atacgcatta cctgccttac cgtttcggcg acaatcaacg cgttatcgaa
     5941 ctgcatacgc ggcagggggt gaaaacgcct gtcgaagtgt cggcggaaat cctcaaaacc
     6001 cttaaatcgc gcgccgaaga aaccttgggc ggcgatttgg tcggcgtggt gattaccgtc
     6061 cccgcctatt tcgacgatgc ccaacgccag gccaccaaag atgccgcgcg tctggcgggt
     6121 ttgaacgtat tgcgcctgct caacgaaccc accgccgccg caatcgccta cgggctggac
     6181 aacgcctcgg aaggcacgtt tgtcgtgtac gacttagggg gcggcacatt cgacgtatcc
     6241 gtattgcaac tgaccaaagg actgtttgaa gtcaaagcca ccggcggcaa cagcgcgttg
     6301 ggcggcgatg acttcgacca tcgtttgttc tgctacctgc tcgaacaaaa cggactctcc
     6361 caactcaacg aacaagacag ccaactcctg ctttcgctcg tccgcgccgc caaagaacaa
     6421 ttaaccacgc aaaccgaagc gcgcattcag gcgacgcttt cagacggcat ggcaatcgac
     6481 acaagcatca gtcgcgccga gttccacaac ctgacgcagc atttggtgat gaaaacgctc
     6541 gaaccggtca aacaggcgtt gaaagatgcc ggtgtcggta aaaacgaagt caaaggcgta
     6601 attatggtcg gcggttcgac ccgtatgctg cacgtccagc aggcagtcgc cacctttttc
     6661 gggcaaaccc cgctgaacaa cctcaacccc gacgaagtcg tcgcgctcgg cgcagccata
     6721 caggcgaacg tcctcgcagg caacaaagcc gacggcgaat ggctgctgct ggacgttacg
     6781 cccttgtcgc tcggtttgga aacctatggc ggcttggcgg aaaaaatcat cccgcgcaat
     6841 tccaccatcc ccaccgcgcg cgcgcaggac tttaccacct tcaaagacgg tcagaccgcg
     6901 atgacgatac acgtcgtaca aggcgaacgc gaactggttg ccgactgccg cagccttgcc
     6961 aaattcaccc tgcgcggcat tccgccgatg gcggcgggtg cggcgcgtat ccgcgtaacc
     7021 ttccaaatcg atgcggacgg gctgctgtcc gtttccgccc aagaacaaag taccggcgta
     7081 cacgcgcaaa tcgaagtcaa accttcctac ggtttggacg acggcgcgat tacccgaatg
     7141 cttaaagaca gtatggacaa cgccgccgaa gatatggcgg cacgcgcccg cgccgaagcc
     7201 gtggtcgaag ccgaaagcct gaccgatgcc gtcaacgccg ccctcgagtt ggacagcgat
     7261 ttgctggatg ccgaagaatt gcaacagatt cggcaaggta tcgccgattt gcaaggccgt
     7321 ctgaaagacg gaaaagccga agacatccgc gccgccgccg ccaaactcgg cagcatcacc
     7381 gacaatttcg ccgccaaacg catgaaccgc aacatccaac gtgcgctgac aggccagagt
     7441 gtcgataata tttgatactt aaacggtttc agacggcata gagataacct gatgccgtct
     7501 gaaggctcga aaacacttga aaaacatcga tatggaaaag tcaggcattg tctatctgat
     7561 gaaaaccgtc atcaagggcg tatataaaat cggcatttcg gaggtaagca attttgaagg
     7621 cagaatgcgc catttggaaa acaacggcta tgcgaacgtt gccgggttgg aacgcatcct
     7681 cgctgtcaaa accgacaatt acaaagaaaa agaaaacctg ctccatgaaa ttttcagcaa
     7741 aagcaggata ggcgataccg aattgttcgc cgtggacgaa aaccttgtga aacgtttgtt
     7801 tttatcgctt cgcggcgaaa tcgtgttccc gaaaaacgaa acggcggaat cggaatttga
     7861 aaaaagcgtc cacgaacgca ggcaggaagg gaatgccggg tcaggccgca aacaactgct
     7921 tgatttggta cggcgcggac accgggaata cccttacgcg ctgccccggc ttttggcggg
     7981 cgcggcatcc tacaagccga gaaaatcgaa aatccgcctt tttaaagaag catatttcgg
     8041 caaaagcggc acgaggctga ccgacgaaat tgcagacggc atccatattt acacctgttt
     8101 ttcgcgggcg gatttggaaa aagcctattc cgaatatttg gaacttttca aatccgaatc
     8161 ggatgccgaa ggcagaaaac cacggtaagg tgcaaacaga taccgtacac gttgaggagc
     8221 agtatgatgg gcgattccgt catttattat gtagaacagg cagacgaacc ggtaaaccgt
     8281 gccggcgaac gcgcccgtaa aacattcaaa tatttttggc gcgagctttt ttgggaacgc
     8341 cgccgcatca ttcccgcctt agattttgcc atggtcaaag cccctttttt ccaagacggc
     8401 gaagacggcg aaatttgcga acatatgtgg atcgacgata tttattttga cggtctttat
     8461 atttacggtg tgctgaacaa taaacccggc gaactgacca atatcgaaca aggcgaaagc
     8521 atttgcgttc cggttgacga catcagcgac tggatgttcg tgtgcaacgg cattccctac
     8581 ggcggcttta ccatacaggc aatgcgtgga cagatgacgg aagaggagcg taccgaacac
     8641 gatgccgcat ggggaatcga tttcggcgat cccgggcagg tattgctggt gtatgaagaa
     8701 aaagaacatc ccgaaaatct ggaagagcat ccgatgtgcc ggaactgtat tgacgatttt
     8761 cggcaacagt tgtcccaaaa tccggatttt ctgcatgagc aggacgaaga cggctatacg
     8821 ccgcttcatc atgaagccat cgcaggaaat gcacttatgg ttcaagccat gcttgaatac
     8881 ggcgcaaatc ctgcctcaaa gacatcggaa ggctataccg ccctcgattt tgcccgcctg
     8941 acgggctggc aaaatgttgc cgacctgctc gaaccgcgac attaggcaga cagttttccg
     9001 aaaacgaaca caaacacttt ttacagaaag acaataaaaa tgcccaaaat caccgtactt
     9061 ccacacacga cattatgccc cgaaggcgcg gttatcgacg acgcgccgga aggcaaaacc
     9121 gtcctcgacg tactgctcga ccacgacatc gaagtcgatc acgcctgcga aaaatcctgc
     9181 gcctgcacca cctgccacgt catcatccgc aaaggtttcg acagtttgga agaaccgacc
     9241 gaattggaag aagacctgct cgaccaagca tggggtttgg aagccgattc gcgcctgagt
     9301 tgtcaggcgg ttgtcgccgg cgaggatttg attgtggaaa tccccaaata caccatcaac
     9361 cacgcgcgag aagaacactg aaaacaggcc gtctgaagcc ggcgcgcttc agacggcatt
     9421 gttgcgcgga taaggcgcaa tcgcccgaaa acaggcgttc gtacaggcgg aaatttcgat
     9481 tctatagtga attaacaaaa atcaggacaa ggcggcgagc cgcagacagt acagatagta
     9541 cggcaaggcg aggtaacgct gtaccggttt aaatttaatt cactatatat tgatttttat
     9601 cggttttctg acggaataat ccagtgcggc atccgaggcg gattactcgg acgcgatgca
     9661 ccggtattta tcggttttgc agccggaaaa accgccggcg ggttatagtg gattaaattt
     9721 aaaccagtac agcgttgcct cgccttgccg tactatttgt actgtctgcg gctcgccgcc
     9781 ttgtcctgat ttttgttaat ccactatact tttagggcga cggtcgggca gtatgccgga
     9841 tagcgttcca ctctcgcttc tatattgatt ttgattggtt ttctgacgga atgacccgat
     9901 gcggcatccg ggacggcttg tgttttttcc tgcccgcctg ccggattttc ccatccttgc
     9961 gtgaaaccga aagagacggc ggcgcggcgg acaagctcga gatagcgtcc ttcaagctcc
    10021 ggacaggcgg cggacacgct ttctaccgta accgtgaaac cgccgcccga cccggcaagg
    10081 cgttccgcaa tttgcgtgta gatgagccaa cgttgggcgc gcaacattgc cgaatcgcaa
    10141 ccggcagccg ctttatccaa ggcaatcagg tcgcgccggt tttggtggtg aaggcaaacc
    10201 gttactctgg agaggatatg ttgcccgtcc aatatttgat acagtttgcg tatcagcaga
    10261 atcaggcggt caaactcctc catctccgac agggaatcag gatggtcgga agcggtataa
    10321 accagtttgg acaattttgc ggcaaacata ttgttcatca atcttccttg tcggttgaaa
    10381 ccccgctctt cggggcggta gaatcagatt tgtttgggag ggacaactcc tcccagatca
    10441 ggacgacaca taggctggtg cttgatgtgt tgtccggcga gttgaaacat tcagcaatcc
    10501 tcaaggggcg gcagttttgc cgaaacatat tctacacggc ttcaatgccg gacgataaaa
    10561 ggaaattcat atgaaatgga ccgacaccca gcgcatcgcc gaagaactct atgacctgca
    10621 cggcgaaacc atcgatccca gaaccgtgcg ctttacccaa ctgcgcgacc tgattatggc
    10681 attgcccgaa tttgacgacg accccgcccg ttgcggcgaa cgcatcctcg aagccgtgca
    10741 gcaggcatgg atagacgagg cggaataagt ttcgggaatg ccgtctgaaa tgcggcggta
    10801 cgcggttcgt gcttctgttt gcagcgggaa tggttttacc agtctccttt tttcagcctg
    10861 tccagttggc ggcggtcgcg cttggtcggt ctgccgtcgg gataggcgga agtgatgcgg
    10921 ctgaattggt cgagctgttt gcgctcttcc ctcaatgttg ccgttttcgc gtcctcttca
    10981 tacagaagcc gcgcctcgga tgccgggcgg cgttggtggt tcaaaccttt aaccttgatt
    11041 ttatagggaa gggaattgag cgtcaggtcg ataatatcgc cgatgtctat ggttttactg
    11101 tttttgacct tcgagccgtt tacttgaacc ctacccagtt cgatgtgctt ttgcgcaagg
    11161 gaacgggtct tgaaaaaacg tgccgcccaa agccatttgt ccagccgcat ggcggaagaa
    11221 tcgtgcttgt ctttcatacg attttgtttg aaataattga atttgtttcg agtttagcat
    11281 aagatacgcc gccttataac tagtatatat gcactaatcc actgttttcc atgctgtccg
    11341 aacacaaaaa gagggtatgg aaaagccgtt ttggacaata aattaactgc ggaatatgca
    11401 caaatagcgt atgatagcgg cagaatctgt tgatgagagc ttcattctat gaaacctgtt
    11461 tttttggatt ttgaacaacc catagccgaa ctgaccaaca aaatcgatga gctgcgtttc
    11521 gtccaagacg agtctgccgt cgatatttcg gacgaaatac accgtttgca gaaaaaaagc
    11581 aacgacctga ccaaatcgat tttcagcaaa ctcacacccg cccaaatttc acaggtttcc
    11641 cggcatccgc agcgtcccta tactttggat tacattgagg cactgtttac cgattttgaa
    11701 gaactgcacg gcgaccgcca ctttgccgac gattatgcga ttgtcggcgg attggcgcgt
    11761 ttcaacggac aaagcgtgat ggtcgtcggg catcagaaag ggcgcgacac caaagaaaaa
    11821 atccgccgca acttcggtat gccccgtcct gaaggctacc gcaaagccct gcgcctgatg
    11881 aagacggcag aaaaattcgg cttgcccgta atgaccttta tcgatacgcc gggcgcgtat
    11941 cccggcatcg gcgcggaaga acgcgggcag tcggaagcca tcggcaaaaa cctgtacgaa
    12001 ctgacgcgcc tgcgcgttcc tgttttgtgt accgtcatcg gcgaaggcgg ttcaggcggt
    12061 gcgttggcgg tcgccgtagg cgattacgtc aatatgctgc aatactcgac ctattctgtt
    12121 atctcccccg aaggctgcgc gtctattttg tggaaaaccg ccgaaaaggc ggcggatgcg
    12181 gctcaggctt tgggcattac tgctgaccgc ctgcaaaagc tggacttggt cgataccgtc
    12241 atcaaagaac cattgggcgg cgcgcatcgg gatttcgggc aaaccatgaa aaacgtaaaa
    12301 gccgttttgg aaaaacaact gcacgaagcg caaagcatcc cgcttgccga tttgctttcg
    12361 cgccgtttcg accgcattat ggcttacggc aaattttcgg aacaataatt caggtagaac
    12421 aagcagcaag cagtttgtct gaaactgctt gctttttctt tatcgggacg gaaccgtgct
    12481 gactttagat gcgtttgagc aatgcttgaa ggattgtttt cctcaaggtc tgaatggaaa
    12541 aaaaacagcg gtggcattaa gcggcggctt ggattccgtc gttttgctgc atctgcttgt
    12601 ccgcgccgga aaaaagggcg gttttattcc ggatgcattg catatccatc acggcttgag
    12661 tccccgtgcc gacgattggg cagatttctg ccaaaactat tgcgatatgc tcggggtggg
    12721 gctggaaacg gttaaggtct gcgtggaaaa aaacggtttg ggcatcgagg cggcggcaag
    12781 gcaaaagcgt tatgccgcgt ttgccgaaaa aggctttgac gttttggcgt tggcgcacca
    12841 cagggacgat caaatcgaaa cctttatgct ggcggtcgcg cgcggcggcg gtttgcgcgc
    12901 tttggcggct atgcccgccg tccgcccttt tggggaaaaa ggcatcatct ggcggccctt
    12961 gctgcctttt tcacgccaag atatatggga ttatgcccaa aaacacggtt tgccgaatat
    13021 cgaggatgaa agcaataccg atacggctta tttgcgaaac cgcttccggc accgtatttt
    13081 gcccgaactt tcggcgcaga ttccccattt cgggcggcat gtgctgaaca atgtccgcgc
    13141 tttgcaggaa gatttggctt tgttggacga ggtcgtcgtt caggactgcc gttgggtttg
    13201 cggggccggt tatttcgata cggcgcggtg gctgacgttt tccccgcgcc ggaaaaccca
    13261 tattttgcgg cattttctga aggaaaacgg cattcccgtg ccgaatcaga atgcccttgc
    13321 cgacattgcc cgggttttga cggaggcaaa aaccggacgt tggaacttgc aaggctttga
    13381 attgcatcat tatgcaggca ggctgtttgt gttccgactg gaaaaaacgg ataaactgcg
    13441 gtttttgaaa gacaggcaga taagcggaaa tttaagggaa atattgacgg ggcagggatt
    13501 tgtgttgaag cggcatccgt ttgggcttcc tgagcatctt ttggagcagg acggaatttt
    13561 gaggacggta gcggcatcgg atacgttggc catgggcggc atccataagg atgtgaaaaa
    13621 aatccttcag gggaaacggg ttttgcctgt cctgcgccca atttggccgc ttgttgccga
    13681 cagcggaaac cgtccattgg cgttggcaaa ctgttgtgcg gatttccaat actcggtttc
    13741 agacggcatt ttgcccgtcc atcctgactt tcccatttta ttttgataat atcgcaaaca
    13801 gatttcggcg gcgttcagtc gggtattgtc cggttgcata tttctaaaag gcttgtgaag
    13861 tgaaacacat cagttcgacc aataatgaac acatcagaca cctgcaccgc ctgttgtcgc
    13921 aaggaaagtt cagacggcaa tacgcccaaa ccgttttgga gggcgtgcac ctgcttcagg
    13981 ttttcctgca atccggcggg atgccggtcg gggtatatat tcccgaagcg aaaatgccgt
    14041 ctgaagaagt ccgtaaattg acggcggttt tgccggaaga cgggtttttt tccgtttcag
    14101 acggcatatt gaagaaaatc agcagcctga cttgtgcgga tgatgtgctt gcgctgattg
    14161 atattccaga tgcgggtgct ttgccggccg gcggcgattg cgtggttttg gacggcgtgc
    14221 aagacccggg caatgtcggc acggtgttgc gaagcgcggc ggcggcggga atcggcgcgg
    14281 tcattttggg caaaggttgt gcggacgcgt ggtcgcccaa agtgctgcga gccggaatgg
    14341 gcgcgcattt cttgtcggag atttatccgc aggcggattt ggaaatatgg ttggtgcgct
    14401 ataaaggccg tgtgtttgcc accgccttgc gcgaggaaaa gcaggcggtt ttgtacggcg
    14461 aagatttgtg cgaaccgaca gcctgggtgt ttggcaacga aggcgcgggg gtcggtaaag
    14521 cagttttaga tagggcggac aagtgtgtca ggataccgat gcacgatgca accgagtctt
    14581 taaatgtcgc gatggcggcg acaatctgcc tgtttgaaca aatgcgccaa cgggcggcgt
    14641 attgaggaag agaaatgccg tctgaaaaaa tctattacgg cgtattgatt ttcttatgta
    14701 tcgcttctat gctgctgtcg ccgttttttt atgcgggtgc tttgaagccc aagaaggcgg
    14761 cattgcggaa ggacgggcag tggaaactca tctgattgtc caatgccgtg gcggcggcgg
    14821 ttttggcttg ggtgtggtgg aaatggtttt gacagatatt gctcaaaatc gtgctaatgg
    14881 aatccgaaca aataaagaat ttggtaaaaa atttgttaaa tcaacgaatt aaagttttgt
    14941 ggaaaacaaa acagctctaa gcaaataggg cgtttgtcgg taaatacgga agagttgcgg
    15001 cattatcggg catctttaac aagtagtgcc gtcttgacag gcaatcggtt tttatgggca
    15061 gcttgcaaaa tcgcggatat aaaattgcga atcggttaaa gtgtggggac gctatgaaaa
    15121 attgcgaatt ttttatgacc cgacaagggc aatctatgat agcggtgcag attacttaac
    15181 tagggaaaaa catagattag tcgtaattgc aaatagtgct tgggggctat tgcttaattt
    15241 atcttgttat tatgacgagg ttttggaaaa gcggaaaata ccgttcggca aacaggaaat
    15301 tgatgacgat atggacaaag tgtccgccct taagcggaag tttaaagata tttctgaaat
    15361 caaagtaggg gatggttggg aatacccgtt caattatgag cagggaatga aagaattaga
    15421 tgaagtgcta ttgaaataca ttcccttttt tgaagaagaa cgataaagga ggttgatatg
    15481 cgcgtatcta aaataattgg aagtatgttg cttgttacag cggttcagac cgtattttcg
    15541 gcaaatgttt acgcgtgccg ccataatggt aaaaccagtt acagccaaac tccgggaaaa
    15601 cattgtacca acgcgggttt ggggcgggac cgggtgtaca gttcggttag accggcagta
    15661 aaagacaggg cggaagacgc aggggtcggc gattattcgg acacggtgag ggacgaacac
    15721 gtccaaaatc cgaaaggaaa tgcacagaaa gacggttcgg ctgccggcat caagccgcac
    15781 tgattgaagc cgaatcagcc cttgcgctgt cggacggcaa aatttgaacg attggggaac
    15841 tttgatgaaa cacatccata ttatcggtat cggcggcacg tttatgggcg ggcttgccgc
    15901 cattgccaaa gaagcggggt ttgaagtcag cggttgcgac gcgaagatgt atccgccgat
    15961 gagcacccag ctcgaagcct tgggtataga cgtgtatgaa ggcttcgatg ccgctcagtt
    16021 ggacgaattt aaagccgacg tttacgttat cggcaatgtc gccaagcgcg ggatggatgt
    16081 ggttgaagcg attttgaacc tcggcctgcc ttatatttcc ggcccgcaat ggctgtcgga
    16141 aaacgtgctg caccatcatt gggtactcgg tgtggcgggg acgcacggca aaacgaccac
    16201 cgcctccatg ctcgcatggg tcttggaata tgccggcctc gcgccgggct tccttattgg
    16261 cggcgtaccg gaaaatttcg gcgtttccgc ccgcctgccg caaacgccgc gccaagaccc
    16321 gaacagccaa tcgccgtttt tcgtcatcga agccgacgaa tacgacaccg cctttttcga
    16381 caaacgttct aaattcgtgc attaccgtcc gcgtaccgcc gtgttgaaca atctggaatt
    16441 cgaccacgcc gacatctttg ccgacttggg cgcgatacag acccagttcc actacctcgt
    16501 gcgtaccgtg ccgtctgaag gcttaatcgt ctgcaacgga cggcagcaaa gcctgcaaga
    16561 tactttggac aaaggctgct ggacgccggt ggaaaaattc ggcacggaac acggctggca
    16621 ggccggcgaa gccaatgccg acggctcgtt cgacgtgttg ctcgacggca aaaccgccgg
    16681 acgcgtcaaa tgggatttga tgggcaggca caaccgcatg aacgcgctcg ccgtcattgc
    16741 cgccgcgcgt catgtcggtg tcgatattca gaccgcctgc gaagccttgg gcgcgtttaa
    16801 aaacgtcaaa cgccggatgg aaatcaaagg cacggcaaac ggcatcaccg tttacgacga
    16861 cttcgcccac cacccgaccg ccatcgaaac cacgattcaa ggtttgcgcc aacgcgtcgg
    16921 cggcgcgcgc atcctcgccg tcctcgaacc gcgttccaac acgatgaagc tgggcacgat
    16981 gaagtccgcc ctgcctgtaa gcctcaaaga agccgaccaa gtgttctgct acgccggcgg
    17041 cgtggactgg gacgtcgccg aagccctcgc gcctttgggc ggcaggctga acgtcggcaa
    17101 agacttcgat gccttcgttg ccgaaatcgt gaaaaacgcc gaagtaggcg accatatttt
    17161 ggtgatgagc aacggcggtt tcggcggaat acacggaaag ctgctggaag ctttgagata
    17221 gcccgggcga tgccgtctga aagcccttca gacggcatcg cccggctgcg cggcacaaag
    17281 gcggaaaaac cgtttgcccc gtattttcaa acgcgttaca cttgccgccg ctgttttcag
    17341 ccatttgatt acccgcaacc gccgtcattg cgccggcggt ttgcctgtca gcgtcattgc
    17401 gccgctgtaa atacgaaaga acacattatg accgtatccc ccgtcgcctt gcgccgtaag
    17461 accgagtgca agcctcatcc caccgcgcgc tattggaaaa aatgcgatgt cgaagccctg
    17521 ttcggacttc ccttcctcga cctcatttac caagccgccg aaatccaccg ccaaaatttc
    17581 aacccgcgcg aaatccagct ttccacgctg ttgtccatca aaaccggcgg ttgtcccgaa
    17641 gactgcgcct attgtccgca atcggcgcac cacaatacca atctgggcaa agagcagatg
    17701 atggatgtgg atgaaatcgt cgaaaaagcc aaaatcgcca aatcgcgcgg cgcaagccgg
    17761 ttttgtatgg gcgcggcgtg gcgcggccct aaacccaaag acgtggagac ggtttccgca
    17821 atcatcaaag ccgtcaaggg cttgggtatg gaaacctgcg gcacgttcgg tatgctcgaa
    17881 gaaggtatgg cggaagactt gaaagaggcg ggcttggatt attacaacca caacctcgac
    17941 accgaccccg accgctacaa cgacatcatc cacacccgcc aacacgaaga ccgaatggac
    18001 accttgggca aagtccgcaa cgccggtttg aaagtctgct gcggcggcat cgtcgggatg
    18061 aacgaaaccc gcgccgaacg tgccgggctg attgccagtc tcgccaatct cgacccgcag
    18121 cccgaaagcg tgccgattaa ccggttggtc aaagtggaag gcacgccgct tgccgatgcc
    18181 gaagatttgg actggacgga atttgtccgc accatcgccg tggcgcggat tacgatgccg
    18241 caaagttatg tccggctgtc ggcagggcgc agcaatatgc ctgaagcaat gcaggcgatg
    18301 tgctttatgg cgggcgcgaa ctcgattttt tacggcgaca agctgctgac cacgggcaat
    18361 cctgatgagg acggcgacag aatcctgatg gaaaagctca acctgtatcc cttgcagttt
    18421 gaaccggaag gcgaggtcgc cgaagtggaa aaagcctttg ggattaaagt ggattattga
    18481 cgattgaaaa atgccgtctg aaacccggaa aaaggctttc agacggcatt tgtccggacg
    18541 gcatttccaa tatcttttta ccggcgcgtg atgctgccgt cgggcgagac atccagcccg
    18601 ttccccttgg ggaaatcgct gcggttcaga taaataatcc gcccgataac ggttttctcg
    18661 gggtcgattt tggggatttt atcgccgact tgagtgatgg ggataatgtt gccggaaacg
    18721 aaccgcccct gtttgtcggt gataatttta aaaatggggg cgatgccgct gatgccggag
    18781 gtgttgattg cgccgtaggt ggcaaagttg ccgccgctgt aggagatgaa gcggtcgcgg
    18841 taaagttcga cggcgcgagt aacgtgcggc ccctgcccga atacgacatc cgcgccggaa
    18901 tcgacggcaa gccgcgcaaa ctcaacgacg ttgcccctgt tttccccata gaagatttcg
    18961 gtatcgaacg gcaggtgttc cgcctgtttc ccttccgcgc cgccgtggaa catcacaatg
    19021 acgatgtcgg ctttctgttt ggttttggta atccgttttc taactttggc ataatcgttc
    19081 agtttgacgg cggcaaggtt gggggcgaag gagacgaagc cggatcttac gccgtttttc
    19141 ttcaggatgg cggtttcaaa cctgttttcg atgcccgaat atttgatgtt caattcgtca
    19201 aggttcgccc tttatgccgt ttccgcaccg caaaccgccg gggtcaagcc ctcggcgcat
    19261 cctgccggaa cggaatcccc gtgctgccga ttgacggttc aaaccccgcg cccgtttcaa
    19321 ataccggcga tgtggacgga caggatgcgc ctgacgaaaa gacagccgat accgtttcca
    19381 ttatcggcgt gggcgacatt atgctcggca gcaattatcc ggtcgattac ctgcccgata
    19441 ccaatattct gaaaaacgtc gaatctgcct tgcaagacgc ggacattacc gtcggcaacc
    19501 tcgaaggcac gctgtttgac gaaggcggta cgccgaaaaa atgtgcaaac ccccaaaata
    19561 tgctatgcat tccgaacgcc ctccgcatac gggcaatacc ttgccgacgc gggattcgac
    19621 tacctcagct tcgccaacaa ccacagcaac gacttcggcg cgcaaggcat cacggcaacg
    19681 gcggcgagcg gcagctcttt tacatactcg atcgcgctaa agccgctgcc gataacgagg
    19741 ccaaaattgc ggaaaatacc gccatcgccc agataaattt gtccatcatc agacctttac
    19801 tgttcagacg agacagcatt tgccgcacgt tttggggctt atctttcgat ttgcgctacg
    19861 tcgcgcaccg cgcctttgtc ggcggaagtc gccatcgcgc cgtaagctct taatgcggcg
    19921 gagacgtagc ggtcgcggtt tttaggcttc catgctttgc tgccgcgcgc ttccatttcg
    19981 gcacggcgtg cggcaagctc ttcatcggaa atggcaaggt ggatgctgcg gttggggatg
    20041 tcgatttcga cggtatcgcc ttcgtgtacc aaaccgatcg cgccaccttc cgccgcttcg
    20101 ggcgaggcgt gtccgatgga caaacctgat gtgccgccgg agaagcgtcc gtcggttaag
    20161 agagcgcagg ctttgccgag gcctttagat ttcaggtagg aagtcggata cagcatttcc
    20221 tgcatgcccg ggccgccttt cgggccttcg tagcggatga tgacgatgtc gccagcgacg
    20281 atttggttgc ccaaaatgcc ttctactgcg tcttcttggc tttcaaacac gcgggcgcgg
    20341 ccggtgaatt tgaggatgct ctcgtccacg cctgcggttt ttaccacgca gccgcgctcg
    20401 gcgatgttgc cgaacaagac cgccaaaccg ccgtcttgcg agtaggcgtg tgccacgtcg
    20461 cggatacagc ctttttcgcg gtcgaggtcg agggttttcc acatacggtt ttgtgagaac
    20521 gcttgggtgg tgcgtacgcc gcccggcgcg gctttgaagc gttcgatggc acgggtgttt
    20581 tcgggattgg tcacgtccca ttgttcaatc gcgtctttca gcgtcggcgc gtggatggtg
    20641 tgcacgtcgg tgtgcagttt gcccgctttg tccagttctt tcaggatggc gaagataccg
    20701 ccggcgcgat gcacgtcttc catatagtag tcgtggttgt tgggtgcggt tttgcagatg
    20761 cagggcacga cgcggcttaa gcggtcgatg tctgccattt tgaaatcgac accggcttcg
    20821 ttggcaacgg ccaacaggtg caaaatggta ttggtgctgc cgcccatcgc aatatccatc
    20881 gtcatagcgt tttcaaacgc ttttttggtg gcaatgctgc gcggtaacac ggtttcatcg
    20941 ttttgctcgt aatagcgttt ggtgatttcg acaatcatac ggccggcttc gaggaacaat
    21001 tctttgcggc cggcgtgggt cgccaaatac gaaccgttgc cgggcaggga aaggccgagt
    21061 gcttcggtca ggcagttcat cgagtttgcc gtaaacatac ccgaacacga gccgcaggtc
    21121 gggcaggcgt tttgttcgac ttcctcgact tgccggttgc tgacattgtc gtccgccgat
    21181 tcaatcatcg cgtcaatcaa gtccaaacgg cgttcgggct ggatgtttgc cacgccgata
    21241 accttgcccg cttccatcgg gccgccggag acgaagatgg tggggatgtt caggcgcatc
    21301 gcggcaatca gcatgcccgg ggtgattttg tcgcagttgg aaatgcacac cagcgcgtcg
    21361 gcgcagtggg cgttgaccat atattcgata gagtcggcaa tcaaatcgcg gctgggcagg
    21421 gagtacagca tgccgctgtg tcccatagcg atgccgtcgt cgatggcgat ggtgttgaat
    21481 tctttggcga ttgcgccggc tttttcgatt tcgcgggcaa ccagctggcc catattgtgc
    21541 aggtggacat ggccgggcac gaattgggtg aaggagttgg caacggcgat gatgggcttg
    21601 ccgaagtcgg tttccatcac gccggtggcg cgccacaatg cacgtgcgcc cgccatattg
    21661 cggccgtggg tggaggtttt ggagcggtat tcaggcatag tgtgtttcct tgtgcctata
    21721 ccgtctgaaa gacagggctg tttcagacgg tatcgggtac ggttttttag agtgggaaaa
    21781 gagggtattt tataccaagt atcggaattt tgcgggattg aaacggcgtg cggcaaaaaa
    21841 gaaaatcccc gcaggaatgc ggggacgggt tcaggcgcgg gcaatcgcga cggctttgga
    21901 tgcgtcccaa aaatcaacgg gtgcatttaa tacgggtttg acgatgcccg tccgtatggc
    21961 gaaatcgccg aaaccttcgc cgatattgcg ttctgccgcc catttgccga tcaggtcgtc
    22021 caattcggca aggatttcgg gcagggtgat gttttctttg taaagacggg ggatgcgtac
    22081 gccttcacgg tcgccgccga tatggaggtt gtagcgtccg acggctttgc cgaccagtcc
    22141 gatttccgcc aacatcgccc gtccgcagcc gttcgggcag ccggtaatgc gggtaacgat
    22201 gtagtcgtcc gacgtgccgt gtttcgccat aatcttatcc agctcgccga tgaagtccgg
    22261 cagcacgcgt tcggcttccg ccattgccag cgggcaggtc ggaaaggaaa cgcaggacat
    22321 cgcattttca cgcagcttgc tgacatcgtt gcggattaat ccgtatgttc gggcaaattc
    22381 ttcgattttt gctttgtctg cttcggcgac atttgccacg atgaggtttt ggttggcggt
    22441 gatgcggaaa tcgcctttgt ggattttggc gatttccaac acgccggtca gaagctgttt
    22501 cccgccttcg tcaaccaaac gcccgctttc gatgaaaagg gttaaatgcc agttgccgtc
    22561 tatgcctttc acccagccga tgcggtcgcc gcgcccggta aatttgaacg ggcgtacggg
    22621 ttcgaacggc atacccatac ggcgttcaac ttccgcgcgg aagttgtcca agcccatatt
    22681 ttgaatggtg tagcgggtgc gggcgttttt gcggtcgctg cggttgccga agtcgcgctg
    22741 cgtggttacc accgcttcgg cggccttcag cgcgtgttcc ggaggcacga aacccagttc
    22801 cagtgaaatg ttcggatagg ttttggtgtt gccgtgttcc atcgaaagcc cgccgcctgc
    22861 caaaacattg aagccggcaa gctgtccgtt accgtctgaa acggcgacga aatccaaatc
    22921 gttgccgtag cagtccacat cgttcaaggg cgggatgacg actgcggttt tgaattttcg
    22981 cggcagatag gttttgccca aaatcggctc gtcttcttga aggaagtcgt cggaactttg
    23041 aacttttttg ccgtccaccc acacatccag ataaccgcgc gtgcgcggca gcaggtgttc
    23101 ggaaatcttt ttcgcgtatt cgtaagcctg ccggtgcagt tcggactcga tcgggttgga
    23161 cgtgcaaagc acgttgcggt tcatatccgc cgccgtggcg atggaatcca aacccagttt
    23221 gtgcaagagg cggtgcatcg tctgcaactt ggctttcggc acgccgtgaa attggaaggt
    23281 ttgccggttg gtcagccgga tggagcggta atgactgttt tcccgggcaa atttgtccag
    23341 ttctatccat tgggacggtt tgatgatccc gcccggcagc cggcagcgca aaagcataaa
    23401 tttcaagggc tcgagttttg cctcggcgcg ttcggcgcgg atgtcgcggt cgtcctgctc
    23461 atacataccg tggaagcgga tgagttggaa gttgtcgcct ttgaagccgc ccgtgagcgg
    23521 gtctttcaaa tcgtccaaaa tcgtgccgcg taaaaaattg ctttcggttt tcagacgttc
    23581 gttgtcggat agcggttttt cttgccacgc caaacctttt gtcttggtct gtacggtcat
    23641 tttgtgttcc tcccgattat atttaatcaa taaacatcac gctgatagcg tttttcttcg
    23701 cgcagcatat ccaaatattc ttctgcgccc tcttcgtcca aatgtcctgc cccgataatc
    23761 acatccagca aggcggcttc cacgtctttt gccatttttg ccgcatcgcc gcacacatag
    23821 atatgcgcgc cttcctgcag ccattgccaa agtccttccg cctgttcgcg gattttgtcc
    23881 tgcacataga ttttttcttc ctgatcgcgg gaccaggcga aatcgtattt atgcaggaaa
    23941 ccctctttgg caaactgctg ccattcggtt tgatagagaa aatcacgggc aaaatgcgga
    24001 ttgccgaaaa acagccagtt tttgcctgtt gcattttctg cggcgcgttg ttgcaaaaaa
    24061 gcgcgaaacg gtgcaatgcc cgtaccggag cctatcatta caatgggttt gcttttgtct
    24121 tcgggcagtc tgaagccgtc gttgcgctcc acaaatacac gcaccgtgcc gtcctcttcc
    24181 agccggtcgg caaggaaacc cgatgcgccg cccgccctgg cgcgcccttc gtgttcaaaa
    24241 cgcaccgcgc cgacggtcag gtgcacttcg tcccccactt ccgcctgcga cgaggaaatc
    24301 gaatacaggc gcggcgcaag cgggcgaagc aggccggcga attgttctgc cgtcaggctt
    24361 gccgggaagc ggtgcagcac atcgacaata ggcgtgtttt gcacgaaatc ctgcaaaacg
    24421 gcgttatcgg caatgatttt atcgagttct tcataatggg cgaacgcggc atagcctttg
    24481 acgaaagccg gggtgttttg cgtgagttcg aaatgagatg aaagtgcgcg cgcaaccggc
    24541 atcatctttc cgcctgcctg tatttccgtt gccggatcga tgccgagcag gtctaggatt
    24601 tccccgacca gtgccggatt gttgtcaaac caaacgccga gcgcgtcgcc tgcctgatag
    24661 cttaaatccg aaccgctcaa atcaagttcg atgtggcgca cgtctttatc ggactggcgg
    24721 gcggtgattt tctgattggc cagcagggcg gcgggaaagg ggtctgcctt agagtacctg
    24781 ccctccggta ccgtctgaag gccggcgggg ggcgttgcct gcggcgcggg tgttgcccgg
    24841 ttttttgcgg cttcttcttt taagagtgcg gcgatattgt ctgtccaggc gtttgcagag
    24901 gcggtaaagt ccaaatccgc atcaacgcgt tcgatcaggc gttttgcgcc caattcttca
    24961 aaacgccggt cgaaatcttt acctgcctga cagaaattcg gataggagct gtcgcccaag
    25021 cccagtacgg caaattggag tttgtccaat ttcggggctt ttttgccgtt cagcagtttg
    25081 tgcagcacga cggcttcttc cggcggttcg ccttcgccct gagtggacgt aaccagcagc
    25141 aggcggcgtt cgtctgcgat gtttttcgcc ttgtagtctt tcagttcggc gcgcctgact
    25201 tgtatgccgg cggctttcag gctgtccgcc gctttgtcgg caacggattt cgcattgccg
    25261 gtttgcgagg cggaaaggac ggtaacggaa aagggttctg ccgccggcaa tgccgtctga
    25321 agcgcgggta gtcctgcaga tgccccgttt cctgcttttg cccaagcgta gccggacagc
    25381 cacgcccatt gtgccgcgtc cagccccgac aggagctgcg tgatttcggg cggcataggc
    25441 ggtaatggcg gatttgtgtt ctgcatatcg tgtccactca taaaatcata cctgccgcaa
    25501 cagtgccgta tgtcgcttcg tctatcagga taaacgaacc ggcggcggtg ttttccgcat
    25561 aaggcgttgc cgtaacgggt ttttgaaggt tgatgcggac tttggcgatg tcgtttatct
    25621 tcaaggattc cgcgcctgcc tcttgttcca gcgtgaggac atccaaaacg ctttcaattt
    25681 ccccgacttt tgccggcagg gtttgcgtgc cgtgcttgag caggtatctg cgcgcggtgt
    25741 tgagcggacg ttcgtcaaac cagcaaagcg tggcttccag atgtttttgc ggggcgagcg
    25801 gggaattttt atcgacaaaa aggtcgccgc gcgaaacatc gatgtcgcga tccagtcgga
    25861 gggttgccgc ctcgccggca aaagcctgcg ccacttcgcc tttgggtgtg atgatttcag
    25921 acactttggc ggtcagcccg ttcggttcga tgcggacggt ttgttcgacg gcgaccgaac
    25981 cgcgttcgat gcgcccctga tagcctcgga aatcatcggc cttgtcggca tcttggcgga
    26041 cgaccagttg cacggggaaa taaaaatcgt cggcggtgcg gctgacttcg tccgcccccg
    26101 gcaggttttc caaaatggac aataagggtt cgcctttata ccaaggcata ttgccgccgg
    26161 ggtaaacgat gttgtcgccc aagagtgcgg acaccggtac gaaatgcacg tctttcaaac
    26221 cgagctgttc ggcaagtcgg cggtatgcct ccacaatggc gttgaatttg tcttcgctgt
    26281 aatccagcag gtccattttg ttgaccgcca ccacaatatg cgggcagttg agttggcgga
    26341 ggatggcgga atggcgtttg gtctgcggca gaagctgcaa gggctgcgcg ccgaaatcca
    26401 gttgggatgc gtcaaccagc acgactgccg ccgaagcggt gcttgcgccc gtaaccatat
    26461 tgcgcgtgta ttgttcgtgc cccggcgtgt cggcgatgat gaatttccgt ttcgccgtgg
    26521 aaaaatagcg gtatgccaca tcgatcgtaa tgccctgttc gcgttcggct tccagtccgt
    26581 cggtcaggat ggagaagtct atggcttctt tcaaaccttt gcttttgccg gattccaagg
    26641 ttttgatttg gtcggacagc agggctttgc tgtcgtagag cagtcgtccg atcagggtgc
    26701 ttttgccgtc atcgacgctg ccggcggtaa tgaagcggag cggggcctgg tgttgtgccg
    26761 tcatattttc ttcctcatat ctgcttaaag ggtttttgaa atttagaaat agccttcttt
    26821 tttgcgtttt tccattgccg cctcgcttgc ctgatcgtct agccgggtcg cgctgcgttc
    26881 ggaaatgtcg gcaaccgccg tttccctgat aatctccgcc ggcgtggacg cggtgctttc
    26941 taccgggcag gtgcagctga tgtcgccgac ggtgcggaag cggacatcaa ggatttcgga
    27001 ggtttcagac ggcattttcg gggtgagcgg cgttacaggg accagcagcc ccctgcgtct
    27061 gaccacttcg cgcctgtggc tgtaataaat cggcggcagc tcgaggtttt cgcgggcgat
    27121 gtattgccag atgtcgagtt ccgtccagtt ggaaatcggg aagacgcgca tattttcgcc
    27181 tttgtgcagc ctggtgttgt acagcgacca cagctcgggg cgttgcgcct tcggatccca
    27241 ttgtccgaac tcgtcgcgga acgagaaaat ccgttctttg gcgcgggctt tttcttcgtc
    27301 gcgccgcgcg ccgcccatca gcgcgtcaaa gccgtttgcc tcgatggttt ccaacaaggt
    27361 aaccgcctgt gccgcattgc gcgaatcggt ttctttgcgt aaaactactg tgcctttggc
    27421 aatggagtct tccacgcgcc ccactatcag gcgggcattg agttttgccg cctgcgcgtc
    27481 gcggaaagca atcacttcgg ggtagttgtg ccccgtgtcg atatgcacca gcgggaaggg
    27541 cagttttacc ggcctgctgc ccagccggaa ggctttgcag gcgagggcga gcaggaccac
    27601 ggaatctttg ccgccggaaa agagcagggc ggggttttcg cattccgccg ccacttcgcg
    27661 gatgatgtgg atggattcgg attccaacca gtcgagttgg gcgttgttcg gttcggtttt
    27721 cgtcatacca tattccttat ttcttctgtc tgatatttat gaattatttg tgcagcccgc
    27781 attctttgct gtttctgcct tcccaccacc accgcccggc gcggatgtct tcgcccgcct
    27841 tgacggggcg ggtgcagggg tcgcagccta tgctgggaaa tccttgccgg tacaaatcgt
    27901 tgtaaggcac attgttggcg aggatgtatg cccacacgtc gtgttccgac cagtcgaaaa
    27961 tcgggttgta tttgccgatg ccccgtccgg catcgtattc ggcaaacggc agttccgtgc
    28021 gtgtggcgga ttgttcgcgg cgttgcccgg taagccaggc gtccgcgcct gcaatggcgc
    28081 ggttgagcgg ttcgattttt cggatgcggc agcattcgcg gcgcgcttca acgctgtcgt
    28141 aaaaggcaaa cctgcctttg ctttccacat aacggtcggc atcttctcga accggccgga
    28201 aacgctttat ccgcaaatgg ggatatgcgc gtccgagcct gtccagcagg ttcagggttt
    28261 ccgtgtggag cagccccgta tccaaggtaa aaatgccgat attgaggttt tcgccggcga
    28321 taaggtcggt aatcaccata tcttctgccg caaggctgct ggcaaaccgt gcatcccggt
    28381 ggctgccgac aatccggtgc aggcgttgtt tgagggtttc cgttttttcc gcaagggcgg
    28441 tttcgccgcc ggatccgata tgcggtatct gccacagggc gggtttgaac agtgtcgttt
    28501 ccatttttcc cgccttatgc cgcccgttgt ccggcattca gtccgcccaa tgcgggatac
    28561 gcctgcccga cccggttttc tccttcgccg ttttcaccga accaggcgag tttttcgtgc
    28621 agccccacca cttcgcctat gacaatcaat gccggattcg gcgcggtttc ggcgagttcg
    28681 gcaaggttgg cgagcgtgcc ggttgcggtt ttttgagccg gcagcgtgcc ttggctgatg
    28741 acggctgccg gcgtgtcggg cgagcgtccg tgctgttgca gccgttcggc aatcagggcg
    28801 gctttgagcg cacccatata aatcaccaag gtctggcggc tgcgggcgag ggtctgccat
    28861 tcgatgtcgg gcgcatccgc cttgcggtgg ccggttacga aaaccgcact ttgggcataa
    28921 tcgcggtgcg tgagcgggat accggcatag gcggtcgcgc cgacggcggc ggtaatgccg
    28981 gggacgaccg aaaacggaat ctgatggcgt gccaaggttt ccaattcttc gccgccgcgt
    29041 ccgaacacga aagggtctcc gcctttcaaa cgcaccacgc gcctgccttc gcgggccagc
    29101 ctgaccataa gcgcattggt gtcctcttgc ggggtgcggt cgccccgggc gcgcttgccg
    29161 acaaaaatcc gttccgcatc gcggcggacg agggacagta tgccgtctga aaccagcgcg
    29221 tcgtaaagca ccacgtctgc ctgctggatt tcctgcagcc ctttgagcgt cagcagcccc
    29281 gcatcgccgg gacccgcgcc gaccaaagac acaaaaccgc cttgataatt ttgttccaac
    29341 tgctttgcca actcctgttc ggcttgggcg gtttgttgat ttttaacgag ggcggcaaaa
    29401 cggccgttga actgcttttc ccaaaaacgg cggcgttcgg taacggattt cagtttggct
    29461 ttgaccgcat cgcgccatct tccggagatt tccgccatat cgcccaaaga atgaggcagc
    29521 agggcttcca gcctttcacg cagcagtcgg gcgaggacgg gcgcgctgcc ggagctggaa
    29581 acggcaatct gaaccgggtt gcggtcgata accgacggga agatgaagct gcaatggtcg
    29641 cggtcgtcca ccacgttgac cggcttttgg cagctttcgg caagatggaa aacgcgccgg
    29701 ttgagggctt ggtcgctgct tgccgcaatg atgaggaaaa ccgtgcggat gtgttcggca
    29761 cgaaattctt cggcaagcca caggattttg ttttccgccg ccaacgcgga gagttcggca
    29821 ttcaggtgtt ttgcggcaac cctgacctct gcgcccgcat tcagcagcag gctgattttg
    29881 cgtgcggcga ctgcgccgcc gcctacgacc aatacggggc ggccggcgag gttggcgaaa
    29941 atggggaaat aattcactgg ctgactcctt tgctgtttgc ccgcaccttg tttccgatac
    30001 ggtgcgtcgc ggcatttttg tcggaacgcg agtcatttta gacaaaagaa tttccccggt
    30061 taaataataa aaaggtattt gttagaagct gaaagctata tgggggcggc tgcggatgcg
    30121 gcggttttcc gttttataac ggtttcggaa gaaaaacggc ctgaagccgt ttcgggcatt
    30181 cagaccgttt gcgtggtgag gggatgccgt ccgaagggcg aaaagggctt cagacggcat
    30241 tgatgtcggg tttcaggaca ggagcaggat ggcggctgcg gcaagcgagg caaccgataa
    30301 tgcggcggca agcgcggctt tgcctgcaaa gcggattgag gttttgcctt cgatgtattt
    30361 gaagccggtt atcatcggga ggatgaggtt tttctttttg aatacgcggt atgcggcgac
    30421 ggcggcgatg tggattgcag aaaaaacggc gagcagcttg aaaaagttga ggtggatttt
    30481 ccgcataagg ctgcccgtat gttcggaaac caaatggttg aggtagccgt tggtgctgaa
    30541 ggtgttttca tcggcggcaa aaagcccggt gccgacttgg aaggacacgg cggccaaaag
    30601 cgcaacgacc atcagtgcgc ccaaggggtt gtgtccgggc tggatgtgtt cgggaatacc
    30661 gtttttcaga tagccgcgta tgcctgccca gccttggacg aaacgggaaa aacgggcggt
    30721 atcgctgccc caaatgcccc agcagaggcg aaatacgagc aggaaaagga cgaacagccc
    30781 gacgcgcgtg tgccattgca gcatatcgcc gccggctttc gcgctatacc acataaaggg
    30841 cagggacgcg gcaagcagcc agtggaaaag gcgggtgggg aggtcccaga ctttggtttt
    30901 gtttttcata atcggtttcc ggcggtagaa tcggtttgtt ttcgagcctt attttaaccg
    30961 attggagggg caatgtttcc cgtttttcat ctttcaggcg agagccgccg ccagatgctt
    31021 cagacggcat tgcgttttcc ccatgttttc aaagcccgtg cggaagattc gcacaaaggg
    31081 actttcggca cgctcgccgt agtcggcgga tcggcaggga tgagcggcgc gcccgtattg
    31141 gcggcatcgg cggcaatgta tctcggctgc ggcaaagtgt gggcgggttt caatcaggat
    31201 acgctacctt ttgccgttat tgccggtttt cccgagatta tgctggatac ggcggacagt
    31261 ttggccaaac gtcaagatat aaacgcctgg gttgtcggtt gtggattggg tacaggtagg
    31321 gcggcggtcg gaacgcttgc cggaattttg acggaacaca cggacaagcc cgtcgttttg
    31381 gatgcggatg cgctgaacat attatcaacc gatgccgaaa cccgaaatct ggcgcgcggg
    31441 tgtaaaaacc tgattttaac gccacacccc gccgaagccg cgcgcctgct tggaacgacg
    31501 gttgcgcagg ttcaggcgga tcggacggcg gcagtgagga agataggggc aattttcggc
    31561 gcaaccgtgg ttttaaaggg gcacaaaaca ttggttgcct cacccgatac ggaaatctat
    31621 gtcaacgaaa gcggcaacgc gggattggca acggcgggca gtggcgacgt attgggcggc
    31681 atcatcggca gtctgctcgc acagggcgtg ccggtttttg aagccgcctg cgcgggcgcg
    31741 tggctgcacg gcgcggcggc ggatgtcata aaagaatcgg caggcattgc ggcagggctg
    31801 ttggcagggg aaatcgctcc ggcggcaagg tggctgcgca accggataac taaaagtatg
    31861 taagaagata tagtggatta acaaaaacca gtac
//
LOCUS       DAXHNE010000007        30409 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.007, whole genome shotgun sequence.
ACCESSION   DAXHNE010000007 DAXHNE010000000
VERSION     DAXHNE010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30409
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.007"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(105..2855)
                     /gene="gyrA"
                     /locus_tag="ACFM4Z_000239"
     CDS             complement(105..2855)
                     /gene="gyrA"
                     /locus_tag="ACFM4Z_000239"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFC6020345.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGLVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            complement(2953..3453)
                     /gene="hscB"
                     /locus_tag="ACFM4Z_000240"
     CDS             complement(2953..3453)
                     /gene="hscB"
                     /locus_tag="ACFM4Z_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001671 - ATPase activator activity
                     [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HFC6020346.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKKSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            3535..3756
                     /locus_tag="ACFM4Z_000241"
     CDS             3535..3756
                     /locus_tag="ACFM4Z_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HFC6020347.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKRGSFRRSRRSL"
     gene            complement(3716..4036)
                     /gene="iscA"
                     /locus_tag="ACFM4Z_000242"
     CDS             complement(3716..4036)
                     /gene="iscA"
                     /locus_tag="ACFM4Z_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFC6020348.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(4123..4509)
                     /gene="iscU"
                     /locus_tag="ACFM4Z_000243"
     CDS             complement(4123..4509)
                     /gene="iscU"
                     /locus_tag="ACFM4Z_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFC6020349.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            complement(4575..4703)
                     /locus_tag="ACFM4Z_000244"
     CDS             complement(4575..4703)
                     /locus_tag="ACFM4Z_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020350.1"
                     /translation="MNEQDLDLDNLDNLLDDFDGVTVEGGVDSENDDGCEGGACKI"
     gene            complement(4770..5984)
                     /locus_tag="ACFM4Z_000245"
     CDS             complement(4770..5984)
                     /locus_tag="ACFM4Z_000245"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HFC6020351.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHSFG
                     WEAEEAVEKARADIAALINADSKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGYEVTYLDVQENGLVDLDVLKAAIREDTILVSVMWVNNEIGVV
                     QDIPAIGEICRERKIIFHVDAAQACGKVPVDVEAAKVDLLSMSGHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYINGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            complement(6013..6459)
                     /gene="iscR"
                     /locus_tag="ACFM4Z_000246"
     CDS             complement(6013..6459)
                     /gene="iscR"
                     /locus_tag="ACFM4Z_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFC6020352.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            6750..7922
                     /locus_tag="ACFM4Z_000247"
     CDS             6750..7922
                     /locus_tag="ACFM4Z_000247"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFC6020353.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            complement(8066..10846)
                     /locus_tag="ACFM4Z_000248"
     CDS             complement(8066..10846)
                     /locus_tag="ACFM4Z_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFC6020354.1"
                     /translation="MANNKMLFEVIENRKAVYLEDGDDEKCRLPEFVERNLKYPFFEW
                     QKSALENFVIFDRTSKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIGSTHAKFLFTEKILQGDTVIPIRKVEMFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHKLNLVMLGDEAHHLNAQTKGKKQGELDLEKEIND
                     RTSNAEIERKGWEHMVLELLLNKNGNPSENVLLEFTATLPENADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLGKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYSAFLNWAENVQADDFEFLDTFSGSLNNSDNANEQGKTRTEQALKFMQENG
                     FEFAHLANWVKQNYQKHNVIITNSETNKNKTEKTDSETEKLLNNLEAADNPIRAIFTV
                     DRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEG
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYISELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFAANKDFRELLIWANKKIPNPNAKTNNANSLQANPQTLSFQVHGNQLLQETQ
                     FTADENDEKVKQLGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFNRLQSKLN
                     IQNRNELQNNLLKDWQIEFLGLGQDKQVRPDDKLAGCLKILEMVEKHLNESDMPFIGT
                     KEFTPKTLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMNNFAGTGLEEALIQFISARL
                     GDLKSQYDIHLIRNEEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVNDFLHYQIFI
                     EPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTES
                     FPLGAASLEKMK"
     gene            complement(10836..12194)
                     /locus_tag="ACFM4Z_000249"
     CDS             complement(10836..12194)
                     /locus_tag="ACFM4Z_000249"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - obsolete DNA methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6020355.1"
                     /translation="MKLIYIDPPYNTGNDGFKYNDKFNHSTWLTFMKNRLEIAKELLM
                     KDGSIFVSIDDNEQAYLKILMDEVFGNENFICNFIWEKKTGASDAKQIATITEFVLCY
                     SKNFKTVKLNKNTFSYDTERYKLSDKFEQERGKYYIDNLDRGGLQYSDSLNFAIQCPD
                     GTFTYPNGRTEFVNDGWIWKWSKNKIDWAITNGFLEFRKSKSKKSGWSVCYKNYMLVD
                     NENKPIERSAPYKNLIQDILNTHATDELKKLFGSKVFTTPKPESLLQYLIQIATSESD
                     IVLDYHLGSGTTAAVAHKMNRQYIGIEQMDYIETLAVERLKKVIDGEQGGISKAVNWQ
                     GGGEFVYAELAPFNETAKQQILACEDSDGIKTLFEGLCERYFLKYNVSINEFSQTIQE
                     PEFQSLPLDEQKQMVLEMLDLNQMYVSLSEMDDEQFAGCLNDDDKALSRAFYQTEKKD
                     GE"
     gene            complement(12422..12862)
                     /locus_tag="ACFM4Z_000250"
     CDS             complement(12422..12862)
                     /locus_tag="ACFM4Z_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6020356.1"
                     /translation="MKTDIQTELTQVLLPHEKVWANEEKTILAKNILLDLVEKTDPTI
                     IGLLLSNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSVNNSYTKYANRIGLTDSNRFL
                     KDSSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRNNSQPANYTLN"
     gene            complement(13079..14002)
                     /gene="truB"
                     /locus_tag="ACFM4Z_000251"
     CDS             complement(13079..14002)
                     /gene="truB"
                     /locus_tag="ACFM4Z_000251"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFC6020357.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHT
                     GVLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIATARAD
                     ISLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKARYITVYAIDI
                     AEFDAPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLNETERDSLLLPCDVLVSHFPQTVLNDYAVHMLHCGQRPRFEEDLPSDTPVRVYT
                     ENGRFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            complement(14035..14406)
                     /gene="rbfA"
                     /locus_tag="ACFM4Z_000252"
     CDS             complement(14035..14406)
                     /gene="rbfA"
                     /locus_tag="ACFM4Z_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFC6020358.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKTPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            14597..15841
                     /gene="clpX"
                     /locus_tag="ACFM4Z_000253"
     CDS             14597..15841
                     /gene="clpX"
                     /locus_tag="ACFM4Z_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFC6020359.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLKGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            complement(15944..17140)
                     /locus_tag="ACFM4Z_000254"
     CDS             complement(15944..17140)
                     /locus_tag="ACFM4Z_000254"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFC6020360.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTKGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGSEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGIVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINTPETLNHVREQGQKLQTALLDLCRKTGLFSQVRGMGLLLGCVL
                     DEAYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLNDEDMAEGLRRLEHALTEFA
                     ATSDNP"
     gene            17544..18044
                     /locus_tag="ACFM4Z_000255"
     CDS             17544..18044
                     /locus_tag="ACFM4Z_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020361.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            18129..18683
                     /locus_tag="ACFM4Z_000256"
     CDS             18129..18683
                     /locus_tag="ACFM4Z_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020362.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            18697..18897
                     /locus_tag="ACFM4Z_000257"
     CDS             18697..18897
                     /locus_tag="ACFM4Z_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020363.1"
                     /translation="MPSEELSDGIFAFVRDIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVMPPLGFQTASYRTVKP"
     gene            complement(19172..20560)
                     /locus_tag="ACFM4Z_000258"
     CDS             complement(19172..20560)
                     /locus_tag="ACFM4Z_000258"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC6020364.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQKLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGWRGDHKPSKEGFGGKTRGEGFKKEGFKRDGFKKTGEGFKGKRKAGDS
                     FAGKGERRYKDR"
     gene            20888..21835
                     /locus_tag="ACFM4Z_000259"
     CDS             20888..21835
                     /locus_tag="ACFM4Z_000259"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6020365.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWAKRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRRFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            complement(21888..22220)
                     /gene="trxA"
                     /locus_tag="ACFM4Z_000260"
     CDS             complement(21888..22220)
                     /gene="trxA"
                     /locus_tag="ACFM4Z_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFC6020366.1"
                     /translation="MSSELIVHTSDAAFEKDVLNADIPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            22521..22991
                     /gene="dnrN"
                     /locus_tag="ACFM4Z_000261"
                     /pseudo
     CDS             22521..22991
                     /gene="dnrN"
                     /locus_tag="ACFM4Z_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein DnrN"
     gene            complement(23062..23883)
                     /gene="nadE"
                     /locus_tag="ACFM4Z_000262"
     CDS             complement(23062..23883)
                     /gene="nadE"
                     /locus_tag="ACFM4Z_000262"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFC6020367.1"
                     /translation="MMRIFSTGGKMDTQAVITHIARWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRPTLLLDMPIRQHPGQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGTHQTAFANQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            24080..25435
                     /gene="xseA"
                     /locus_tag="ACFM4Z_000263"
     CDS             24080..25435
                     /gene="xseA"
                     /locus_tag="ACFM4Z_000263"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFC6020368.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSLLENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAVRLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPVRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGAGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIESCTVPVVSGVGHETDFTLADFVADMRAPTPTGAAELVSPNRQESLHRLAQ
                     AQCRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMTQNVRT
                     HTARFERQTQALKHCRPDVSVCKKNIDRFQTALSHAFHQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHIIFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            25728..27269
                     /locus_tag="ACFM4Z_000264"
     CDS             25728..27269
                     /locus_tag="ACFM4Z_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HFC6020369.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNTFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSIGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMACLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(27338..28387)
                     /locus_tag="ACFM4Z_000265"
     CDS             complement(27338..28387)
                     /locus_tag="ACFM4Z_000265"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC6020370.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETGKTASQPY
                     GQKASDGIKPQNVPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWITNGWITVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRIGFGPIGLPNRLKRGQFYELNPAEVANIIK
                     WADMLLPGERRRKKA"
     gene            complement(28396..28585)
                     /locus_tag="ACFM4Z_000266"
                     /pseudo
     CDS             complement(28396..28585)
                     /locus_tag="ACFM4Z_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424398.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28612..29244
                     /gene="pdxH"
                     /locus_tag="ACFM4Z_000267"
     CDS             28612..29244
                     /gene="pdxH"
                     /locus_tag="ACFM4Z_000267"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFC6020371.1"
                     /translation="MDLHNIREDYSKRELSEADCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLLEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            complement(29374..30384)
                     /locus_tag="ACFM4Z_000268"
     CDS             complement(29374..30384)
                     /locus_tag="ACFM4Z_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFC6020372.1"
                     /translation="MNHTVTLPDQTTFAANDGETVLTAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMGGHSEQALSEAEKAQGKILMCCTTAQSDININIPGYKADALPVRTLP
                     ARIESIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANLPDQEGILE
                     LHIRRHENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQGSNRAVHFYWGARHQDDLYALEEAQGLACRLKNACFTPVLSRPGEGW
                     QGRNGHVQDIAAQDHPDLSEYEVFACGSPAMTEQTKNLFVQQHKLPENLFFSDAFTPS
                     AS"
BASE COUNT         6801 a         7431 c         8107 g         8070 t
ORIGIN      
        1 tgtttttaat tgactataat ccgctatatt gtgagaagct ggatgacgga tgagggataa
       61 aataaatgcc gtctgaatct tcagacggca tcgggagatg attttcagtt ctcgacttcc
      121 ggttcggtta cattggaaat tacagaagcg tcggagagtt cggattcgtc ttcggcaaca
      181 cgttccagcg ataccaaggt ttcgccttcg tccaagttaa tcagtttcac gcctgctgcg
      241 gcgcggccgg tttcgcggat ttgttcgact ttggtgcgga taagtacgcc gccgctggta
      301 atcagcatca aatcgtcggt ttcgccgacc aaggttgcgg cgaccaaatc gccgtttcgc
      361 tcgccggtgt taatggcaat attgccttgc ccgcctttgt ttttgcggct gtaatcggca
      421 atcggggtgc gttttccgta tccgttggcg gtggcggtta aaacttgcaa accgctttct
      481 tcggtttcag gggcgaaggt aatcaggctg acgattttgc cgtcggcagg caggcgcata
      541 ccgcgcaaac cgccgctgcc gcgaccggac gggcgaacac cgtgtttgcc gctcggcagt
      601 gcgttttcgc tgtcggcggt ttcatcttcg atgccgtctg aaatttcggt ttcgatgtcg
      661 gcatcttccg cttcgtcgtt gccggatttt tcccagtatt cgttgaagcg gatggcttta
      721 cctaagttgg agaacagcat gatgtcgtcc gcaccgcctg tttgcgcagc gccgacgagg
      781 tagtcgcctt ctttgagcgc gatggcttta atgccttggg cgcggacgtt tttaaaggcg
      841 gaaagttgga cttttttcac cagtccctgc gcggtggcga agaagacgta ttggtcttcg
      901 gggaactcgc gtactgccag aatcgcgctg actttttcgc cttcttccaa ctggatgacg
      961 ttgttaatcg gacggccgcg gctgttgcgt ccgccttcgg gcagtttgta aaccttaatc
     1021 caatgacact tgcccaaatt ggtaaagcac atcaaataat catgcgtgtt ggcaacaaac
     1081 agggtttcga taaagtcttc gtctttggtg gcagccgcct gtttgccgcg cccgccgcga
     1141 cgctgcgcct gatagtcggt ggtcggctgg gttttgatat agccgccatg tgtcagggta
     1201 acgaccattt cgcgttgcgg aatcaggtct tcatcggcaa tgtcgccacc gaacgggttg
     1261 atttcgctgc ggcgttcgtc gccatagttg gttttgattt cttccagttc gtcgcggatg
     1321 atttgggtaa tgcgttcggg tttggagagg atatccacaa agtcgatgat tttacccatc
     1381 aggtttttgt agctttcgac aatttcttct tgatcgaggc cggtcaggtt tcgcaggctc
     1441 atgcgtaaaa tagcatctgc ctgaatctcg ctcaggtaat aaccttgctc tttcaagccg
     1501 atgtttgcag ccaatccttc cggacgcatc atttccaaat ccagaccgga acgcgtcagc
     1561 atttcttcaa cgaggctgct gcgccaagga cgtgcaagca gtttgtcttt ggcctcggct
     1621 gcgttgggcg attctttgat gagcttgatg atttcatcga tattggacag tgcgacggct
     1681 ttgccttcgg cgatatgccc ttcatggcgt gccttcttca gccggaaaag cgtacgtcgg
     1741 gtaacgactt cgcggcggtg gcgcaggaat tcggagagaa tctgtttcag gttcaacagg
     1801 cgcggttgtc cgtcgaccaa aaccaccata ttgatgccga aactgtcttg cagcggagtc
     1861 agtttgtaga gttggtttaa gacgacttcg gcattttcgt tgcgtttcag ctcgataacg
     1921 acgcgcatac cggatttgtc ggattcgtcg cggagctcgg aaatgccttc cagtgttttt
     1981 tcccgaacca aatcgccgat tttctcgacc agcttggctt tgttgacctg ataggggatt
     2041 tcgtcgataa cgatggcttc gcgttcgccg tttctgccta tgggttcgat atgggtctta
     2101 ccgcgcataa cgacgcggcc gcggcctgtt ttatagcctt cgcgcacgcc gctcaagccg
     2161 tagatggttg ccccggtcgg gaagtcgggg gcttggataa tgtcgatcag ttcgtcgatt
     2221 tcggtgtcgg gtgcatcgag caggcgcagg caggcattga cggtatcgga aaggttgtgc
     2281 ggcgggatat tggtcgccat gccgacggcg atgccggacg agccgttgac gagcagtgtg
     2341 gggaaacggg tcggcagtac aagcggctcg tgttcgctac cgtcgtagtt cgggccgaaa
     2401 ttgacggttt cttcctcaat gtctgccagc atttcgtggg aaattttcgc catgcggatt
     2461 tcggtgtagc gcatggctgc ggcggcaagc ccgtccaccg atccgaagtt gccctgtccg
     2521 tctatcagca cataacgcat agcgaaattt tgcgccatac ggacgatggt gtcgtatacg
     2581 gcggtatcgc cgtgggggtg gtatttaccg atgacgtcgc cgacaatgcg cgccgatttt
     2641 ttgtaggcgg cattccagtt gtttttcaat tcgtgcatcg cgtacagtac gcggcggtgt
     2701 accggcttga gaccgtcgcg aacgtccggc agcgcgcgcc cgacaatgac gctcatggcg
     2761 tagtcgagat agcttttgcg catttcgtct tcaaggctta ccggcagggt ttcgagggcg
     2821 aatttgtggt cgtggcggat ggttgcgtcg gtcatggttt caatgtttcg tatggcaaaa
     2881 aattgttgct tattttagca tattttgacg cggaacggtg cggcggttac gccgtctgaa
     2941 acacggtgcg gattataatg ccgaggaaat ttcgttgcgg agtttgtcga gaaacctgcc
     3001 ttggcggact tgttgtgcgg ctgtgtcgta atcttgtcgg gcaaacgact gtttcagacc
     3061 gcagaacagt ttttcttgct cgtcgcggat ttcgttgtcg agatttttca aggattcaag
     3121 gtcgttgcct gcccgtgcct ccatcagcgt ttcgcgccat tccatttgct gcataaggaa
     3181 ttcgggggcg aaagcggtat gctccggcgc gtcggcatcg atgcccgatt ttttcagcag
     3241 gtaggcggcg cggtcgatgg ggtttttcaa ggtgcggtag gcatcgttga tggtggaaga
     3301 catcatcact gcctgctttt gctcaaaggc ggaagctgaa gcgaatttat cgggatggaa
     3361 acgggcggcc aaggcgcggt aggtttgttc caagttttcg gtgtcgatat cgaaagcggg
     3421 ttcaatccgg aagagggtga aatattggga catagtagga tgataaatgt aagattttgg
     3481 cagaaaactg tttttgcctt ataatctgca gcttcttaaa cgaaaggact gaatatgggc
     3541 ggcaaagtgc agcacaataa aggcaaaata cgcgacaatg ctttaaaagc cttagtgaaa
     3601 tccgatttgt tccggcacaa ggtggaacgg aaaaggaaag gcaaaggcag ctacaacagg
     3661 caggaagcga aaaaatggcg ggacggtttt gatactgtcc cgccgttttt atgccttaaa
     3721 cgtggaagct ttcgccgcag ccgcaggagt ctttgacatt ggggttttca aatttgaaac
     3781 cttcctgcaa accttctttg gtgtaatcga cttgcgtgcc gtccagataa accaggcttt
     3841 tcggatcgat ataaatgcgc gcgccgtgtc cttcgaaaat caggtcgtcg ccatcggctt
     3901 catcgacaaa ttcaaggttg tacgccatcc ccgagcagcc gctggttttc acacccaagc
     3961 gtacgcccaa gcctttgccg cgtttggcga gatagtcatt gatgtgtttt gcggcattct
     4021 cggtaagggt aatcatattt cttccttgtt gtaccgcccc ggacggacct gaagcggcgg
     4081 gtggttcgga cggcattgcg ggacgatgcc gtctgaaggg ctttatctgt tttcctgacg
     4141 tttgcggtag tcggcaacgg ccgcttttac cgcgtcttcc gctaagatgg agcagtggat
     4201 ttttaccggc ggcagttcca actcctcggc gatttcgctg tttttgattg ccagcgcgtc
     4261 atccaggctt ttgcctttga cccactcggt aatcaggctg gacgaagcga tggcggaacc
     4321 gcagccgtaa gttttgaatt tcgcatcttc gatgatgcct tcatcgttca ctttgatttg
     4381 caggcgcatc acgtcgccgc aggccggtgc gccgaccatg ccggtgccga cagattcgtc
     4441 gtttttgtcg aaagtaccga cgttgcgggg attttcgtag tggtcgatta ctttatcgct
     4501 gtatgccatg atgtggtttc cttaatgttt tgatgattta agtggtttgt ttgccggttt
     4561 tcagacggcc tgagttagat tttgcacgct ccgccttcac agccgtcgtc gttttccgaa
     4621 tcgacgccgc cttccacggt aacgccgtcg aaatcgtcaa gcaggttgtc gaggttgtcc
     4681 aaatctaaat cttgttcgtt catatttttc tcttgattgc tttgtttttg gaatgccgtc
     4741 tgaaggttcg gatggcatgt tggtacgctt taatgcgccg cccattcaat cgagttcaaa
     4801 tcaatcccgt ctttgaacat ttcccacaac ggcgacagtt cgcgcagttt gccgattttg
     4861 gatttaatca gttcggcggc aaattgcact tcttcttcag tggtcatgcg accgaaggtg
     4921 atgcgcaggg acgagtgcgc caattcgtca tttctgccca atgcgcgcag gacgtagctg
     4981 ggttcgagcg aggcggaggt gcaggcggag ccgctggata cggcgagttc tttcactgcc
     5041 ataatcaggc tttcgccttc gacgaagttg aagctgacgt tcaggttgtt cggggcgcgg
     5101 tgttcgaggt cgccgttgat atagacttct tcgatgcctt cgatgccttt gaggaagatg
     5161 tcgcgcagtt tgcggtagtg cgccatatct tgttcgagtt cttctttggc gatgcggaag
     5221 gcttcgccca taccgacgat ttggtgggtc ggtaatgtgc cgctgcggaa gccgcgttcg
     5281 tggccgccac cgtgcatttg ggcttcgagg cggacgcgcg gtttgcggcg gacgtacagc
     5341 gcgccgatgc ctttagggcc gtatactttg tggccggaca tagacaacaa atcaactttg
     5401 gcggcttcaa cgtcaacagg tactttgccg caggcttggg cggcatcgac gtggaaaatg
     5461 attttgcgtt cgcggcagat ttcgccgatg gcgggaatat cttgaaccac gccgatttcg
     5521 ttgtttaccc acattacgga aacgaggatg gtgtcttcgc ggatggcggc tttcagtacg
     5581 tctaaatcaa ccaaaccgtt ttcttgtacg tccagataag ttacttcgta accttggcgt
     5641 tcgagttcgc gcatggtgtc gagtacggct ttgtgttcgg ttttgacggt gatgaggtgt
     5701 ttgcctttgg ttttgtagaa gtttgccgcg cctttgatgg cgaggttgtc ggactcggtc
     5761 gcgccgctgg tgaaaacgat ttctttagag tcggcgttaa tcagggcggc aatgtctgca
     5821 cgtgcttttt ctacagcttc ttctgcttcc cagccgaagc tgtggctgtt ggaggctggg
     5881 ttgccgaagg tttcggtcag atagggaatc attttttcgg caacgcgttt gtcaacgggg
     5941 gtggtggcgg cgtagtcgag gtaaacgggg gttttgacgg tcatggtttg ctctttcttt
     6001 ttcgggtgtt atttaatgga tgtgtgtaaa ttggacgacg cggctgccgt cgccgttgtt
     6061 tttctgttcg atgatgcttt gcagggtaac gctgccgagg tagtcgttga tggttttgtt
     6121 taaattctcc caaagatcgt gcgtcaggca gggcgcgccg tggtggcagt tggctttgct
     6181 gccgcattgg gttgcgtcca gccggtcttc ggcggcggcg atgatttggg cgatgttgat
     6241 gcgtgccgcc ggtgcggcga ggatgtagcc gccgccgggc ccgcgcaggc tttcaacaag
     6301 tccggcgcgg cggagtttgc cgaacaattg ctcgagatag gagagggata tgttttggcg
     6361 ttcgctgatg gcactgagtt tgacggcgcc ggtttgcgcg ttcatcgcca aatccagcat
     6421 agcggtaacg gcgaaacgcc ctttggtggt cagtctcatg gtggtggttg cccatgtcgg
     6481 tttttttatt agtgggtgga attgtccaat atctgagtgt ttcagtcaag tatgagggcg
     6541 gattgtgtga ttggctgttt aaatcgatag attgattgat ttttaatatt ttgattttaa
     6601 tttgttattt ttgaatttta aaccttatgt cgcttaaagt agggagctgc ggcggatgcg
     6661 gcaaatgttg aaatgatttg ccttataatc aatattgata tacattatcg atttcatata
     6721 aagttattca ggtattcagg gggcgacaaa tgaaacgtga tttgagcaaa atgacctgca
     6781 tcgaagactt gcgccgcgtt gccaagcgca aaatgccgcg tatgttttac gattacatcg
     6841 attcgggttc gtggacagaa actacctacc gtgaaaacac ttcggatttc aaagacatcc
     6901 gcttccgaca aaaggtattg gtcaatatgg aaggccgaag cctggaaacc aaaatgatcg
     6961 ggcaggatgt gaaaatgccg gtggcgattg cgccgacggg tttcacgggt atggcacacg
     7021 ccgacggtga aattttggcg gcgcgggcgg cggagaagtt cggcattccg tttacgctgt
     7081 ccacgatgtc tatctgctcg attgaagacg ttgccgagaa cacttccgca ccattttggt
     7141 ttcagcttta tgtgatgcgc gaccgcgagt ttatggaaaa cctgattaag cgcgcgaagg
     7201 atgccaaatg ttcggcattg gtattgaccg ctgatttgca ggttttgggg caacgccaca
     7261 aagacatcaa aaacggcctg tccgcgccgc cgaaaccgac catcgccaat ttaatcaatc
     7321 tggcgaccaa gcccgaatgg tgtatgaaaa tgctgaacac ggaacgccgc acgttccgca
     7381 atatcgtcgg acacgcgaag aacgtcggcg atttgtcttc gctgtcttca tggacttccg
     7441 aacaattcga cccgcgcctg agctgggacg atgtggcgcg cattaaagat ttgtggggcg
     7501 gcaagctgat tatcaaaggc attatggaac ccgaagacgc ggaaaaagca gcgaaaagcg
     7561 gcgcggacgc attgattgtt tccaaccacg gcggccgaca gctcgacgat accgtgtctg
     7621 ccatcaaagc cttgcccgac atcgtcagcg cagtgggcag cgacatcgaa gtttggatgg
     7681 acagcggcat ccgcagcggt caggatattt tgaaggcttg ggctttgggc gcgaaaggta
     7741 caatgatagg tcgggcgttc ctgtatggct tgggcgcgta tggcgaagaa ggcgtaaccc
     7801 gtgcgctgga aatcctatat aaagaaatgg atatatctat ggcgtttacg ggacaccgcg
     7861 atattcaaga tgtcgatgcc tcgattttga ggggtaagga ttggggtagg gaaacggttt
     7921 agatactgaa aaatacgatg ccgtctgaac acagggttca gacggcatta aaatttggct
     7981 gtattttaat aatgtgttga ttaaatatct gacaaaacgc ccttttgtaa aggaagtttt
     8041 gtcaaacggg gctgcctgca atgcactact tcattttttc aagcgatgcc gcacctaaag
     8101 ggaatgactc cgtaaattgt ccatttttct gatgatcagt aaaaaacggt aaaccgatca
     8161 aacgataatg cggtgtatct ttttgcagga tcttatccat cccatattcc acagtgatgg
     8221 cttctaaaaa ttcttctttc catccatcag tttccactaa atgctcacct ttgggctcaa
     8281 tgaaaatttg gtaatgcaaa aagtcattca cgccattgga agaagatttt tgtttatctt
     8341 tcagcaataa aataaagtcc ggcataaatc cttcaccatc ggcaaagtta ttcaatttaa
     8401 acacttcttc attacggatt aaatgaatgt catattgaga tttcaaatcg cccaaacgcg
     8461 ctgaaataaa ttgaattaac gcttcttcca aacccgttcc ggcaaaatta ttcatcacat
     8521 accaatcatt ttgcgtggca atagtcgttt ttacatcatc ttttttgacc catttttgtt
     8581 ttggtgtgcc gaaaatttcc cataatgttt taggcgtaaa ttcttttgta ccgataaacg
     8641 gcatatcact ttcattcaga tgtttttcaa ccatttccaa gattttcagg cagcctgcaa
     8701 gtttatcgtc cgggcgaacc tgtttgtctt gccctaatcc taaaaattca atttgccagt
     8761 ctttcaacaa gttattttgc aattcattgc gattttgaat gttgagcttg ctttgcaaac
     8821 ggttgaaatg gaataaagat tgactatttt tccctttgat atgcagggct ttattgaaaa
     8881 tgtgccgctc catttcactc atttttatgg tttgagtaaa attattttgc gtgccaagtt
     8941 gcttgacttt ttcatcattt tcatcggctg taaattgcgt ttcctgcaac agttgattgc
     9001 cgtgaacttg gaatgaaagc gtttgcggat tggcttgcag gctgtttgca ttattggttt
     9061 tggcgtttgg gttggggatt tttttattcg cccaaattaa caattctcta aaatccttat
     9121 tagcggcaaa ttcagatttg agtttaaatg ttgccaatac cttatcatcg tctttttcag
     9181 gcaaataacc gtcttttcgc aactcgtttt tcagttctga aatatagcgg gattgctcat
     9241 cgtgcgtgta ataaaacaat tcttccaaga tacgcaattc gtgttgcata tcgttgtcaa
     9301 atttgcgttt attcggctgt ttaccttcaa acgcaaatgg aaaataacgc acgccacgac
     9361 caatcaactg cttttccgat acggtagcgg cagccgtttt gcctgatttt ttatttgaac
     9421 caccgccatt ttgcccttca tacaaacgca caatatcaaa caaattcaga acgtcccaac
     9481 cttcggttaa tctatctacc gtaaaaatgg cacggattgg attatcagcc gcttccaaat
     9541 tgtttagcaa tttttcggtt tcgctgtcgg ttttttcagt tttgtttttg ttggtttcgg
     9601 agttggtaat aatcacatta tgtttttgat aattttgttt cacccaattt gccaaatgtg
     9661 caaactcaaa gccattttcc tgcataaatt ttagggcttg ttcggttcgg gttttgcctt
     9721 gttcgttggc gttgtcgcta ttattcaggc tgcctgaaaa cgtatctaaa aactcaaaat
     9781 catccgcttg tacattttct gcccaattta aaaatgccga ataatccgct tttgattcat
     9841 caatcgtctt acttctaaac aacatcacag gcttgaaatt ggcaatgccg tatttcaacg
     9901 cgatttgatg gcgataccaa gcaaataata aagcgtgcaa cacccgctct ttcttgccca
     9961 gcgtactgga taccaaattg atttctttgg tataaccttt ttgcaaaaat tcttttaagc
    10021 caaattttgt gatgatttta tccgcgtatt tttgttgcac gtcagcattt tcaggtagcg
    10081 tggcggtaaa ttccaacagc acattttcgc taggattgcc atttttattg agtaacaatt
    10141 ccaaaaccat atgctcccag cctttgcgtt caatctcggc attgctagtg cggtcgttaa
    10201 tttccttttc taaatctaat tcgccttgtt ttttgccttt ggtttgtgcg tttaaatggt
    10261 gtgcttcatc gcccagcatc acaaggttca atttgtgcaa atccgccaat gtggtttggt
    10321 tttcccgttc ggtgtggata tcgttataca gcttttgaat gctggtaaat ttaatttcaa
    10381 tgccgtctga atgtgggctg aacatttcca ctttgcgaat gggaattacc gtatcgccct
    10441 gcaaaatctt ctcggtaaat aaaaattttg cgtgcgttga gccaataaaa ttattttccg
    10501 ttttatctac gatattgttt tgattgacga aaaacagaaa atgccgataa cccttttcaa
    10561 aataatacaa aatcaacgcc gccatcatca tcgttttgcc tgcacctgtt gccatattga
    10621 acagcaaatg ggtagggcgg tttttgatat cgggaaaatc ctttaatttt gaagtgcggt
    10681 caaaaatcac aaagttttct aaagccgatt tttgccattc aaaaaacgga tattttaaat
    10741 tgcgttctac aaattcaggc agcctgcatt tttcatcatc gccgtcttcc aaatacacag
    10801 ctttgcggtt ttcaatcact tcaaacaaca ttttattatt cgccatcttt tttctccgtt
    10861 tgatagaacg cacggcttaa cgctttatcg tcatcattca gacagcctgc aaattgttca
    10921 tcgtccattt cggataatga aacatacatt tgatttaaat ccaacatttc aagcaccatt
    10981 tgtttttgtt cgtctaatgg caaagattga aattcaggct cttgaatggt ttgactaaat
    11041 tcatttatgc tgacgttgta tttcaagaaa tagcgttcgc ataaaccttc aaacagcgtt
    11101 ttgatgccgt ctgaatcttc gcaagccaaa atctgttgtt ttgcggtttc gttaaatgga
    11161 gcaagttcgg cataaacaaa ttcgccacca ccttgccaat tcacggcttt ggaaataccg
    11221 ccttgctcgc catcaatcac ttttttcaag cgttcaacag caagcgtttc aatataatcc
    11281 atttgttcaa taccgatata ttggcggttc attttgtggg caacggcggc ggttgtgcca
    11341 ctaccaagat ggtagtctaa gacgatgtcg gattcggatg tggcaatttg aataagatac
    11401 tgcaataagc tctcaggttt tggagtagta aaaactttgc tgccgaacag ttttttcaat
    11461 tcatctgtcg catgtgtatt taagatatcc tgtattaagt tcttataggg agcagaacgt
    11521 tctatcggct tgttttcgtt atcaaccaac atatagtttt tataacatac actccatcca
    11581 gattttttag actttgattt tctaaactcc aaaaaaccgt ttgttattgc ccaatcaatt
    11641 ttatttttac tccatttcca tatccagcca tcattgacaa attcagtcct gccattcgga
    11701 tacgtaaaag tgccatctgg acattggatt gcaaaattca aactgtcact atactgcaat
    11761 ccccctctat ctaaattgtc gatataatat ttgcctcttt cctgttcaaa cttatcactt
    11821 aatttgtatc tctctgtatc ataagaaaac gtgtttttat ttaatttaac tgttttaaag
    11881 ttctttgagt aacataagac aaactctgta atagtcgcta tctgtttggc atcggacgca
    11941 cctgtctttt tttcccaaat aaaattgcag atgaaatttt catttccgaa aacttcatcc
    12001 attaaaattt tcaaatatgc ctgttcgttg tcgtcaattg acacaaaaat cgaaccgtct
    12061 ttcataagca gctctttggc gatttctaga cggtttttca taaaagtcag ccaagtggaa
    12121 tgattaaatt tgtcgttgta tttaaaaccg tcattacccg tgttatatgg cgggtcaata
    12181 taaatcagct tcactttgcc tttaaactgc ttggcaagcg aatgcagggc aatcagatta
    12241 ttgcctttga taatgagatt ttcggcgggt gtgccgtctg aatgtcgttt gatttcgcca
    12301 acggcttgtt tgccgtctgc ggtatagcga gagaattttg agaatgcttt tgcgtcaaaa
    12361 agccgatcaa tttcatcaaa agcaagggtt tgattaaaaa agatttcttg tctttttcgg
    12421 gttaatttag tgtataattg gctggctggc tgttattgcg tttaaaataa atttcttcgc
    12481 cttcctcggt gctttgtccg ccatttaaca cacaatcttt aaacggaaaa tccaacacaa
    12541 tatcactgct gtctttcaaa aagcggttgc tgtccgtcaa accaatgcgg ttggcgtatt
    12601 ttgtgtagga attattgacg ctgtgtttgt ccaagaaaaa acggaaatcc tgcaatttaa
    12661 acaccagcac accattcact tccacaaaga aatggcgttt taactcatca ttactcaata
    12721 acaaaccgat aatggtcggg tcggtttttt ccaccaaatc caacaggatg tttttggcta
    12781 aaatggtttt ttcttcgttc gcccatactt tttcgtgtgg tagtaggact tgggttaatt
    12841 cggtttgaat gtctgtcttc atcgttataa atcctcatca gttattttaa tgtgccattc
    12901 ttgtgtaacg gcaaatggct ttgcaaatag attctaataa aatcaattat ttgatgtctt
    12961 tgctgtttcc gggggcgttc gcaaagttgc gataagcggg gcgggtgtcc gagtgtgtcg
    13021 ggattttgcg gaaatatcaa cacattattc aagcacagcc tgtattttta accgccgttc
    13081 aggcggcgga tgccgccgtg ttcatcaggc gcaaggcttt cagacggcat atttcttttt
    13141 gatattccgc cagaccgaca aagcggccgt tttccgtgta aacgcgtacc ggcgtgtcgg
    13201 aaggcaggtc ttcttcgaaa cgcggacgtt gtccgcagtg gagcatatgg acggcataat
    13261 cgtttaaaac ggtttgggga aagtgtgaaa ccaatacgtc gcagggtagc agcaagctgt
    13321 cgcgttctgt ttcgttcaaa tttgccaagg cctcaagcgt gtggctttgg gcgatggtaa
    13381 agccggcggt ttcagtgcgg cgcagggcgg tcaggtgggc gaacgtgccg atgtgtttgg
    13441 cgatgtcttc gctgagggtg cggatgtagg tgcctttgct gcaacgtacg tcgatgacgg
    13501 ctttgggcgc gtcaaattcg gcaatatcga tggcgtaaac ggtaatgtag cgcgctttgc
    13561 gttcgatgac gatgcctttg cgggcgtatt cgtacagcgg tttgccttcg tgcttgagcg
    13621 cggaaaacat tggcggcact tggcggatgt tgcctgtcag tgcttggcag gccgtctgaa
    13681 attcggctaa ggaaatatcg gcgcgggcgg tggcaatgat ttcgccttcg gcatcacccg
    13741 tgctgctggc ttcgccgagt ttcagcgtgg cggtgtaggc tttgtcggca tccagcaggt
    13801 attgggcgaa cttggtcgct tcaccgaagc agacgggcaa aagtccggtt gccaaagggt
    13861 cgagcacgcc ggtatgtccg gctttttcgg catgaaacaa acgccgcgct ttttgcagcg
    13921 cggtgttgct ggaaaggcct tcgggtttgt ccaagagcaa aacaccgttg acggcacgtt
    13981 tttggggttt gcctgtattc atggttcgat gttcggacgg catgggcata tccgtcagtc
    14041 ttcaaccggt ttttccgccg ctacttggtc gataagggcg gacaggttca aaccgcgttc
    14101 caaagattcg tcgtatttga aatgcagttc gggcgttttg aacagcttga tgcgtttggc
    14161 caattcgctg cggaggtgtc cgcgcgcgtg ttccagcact tcttccgtaa tttcgcgcgc
    14221 gtcttggttt aaaatggtgt agaacacggt ggcgtggctg taatcgcggg taacttcgac
    14281 ttcgttgacg gtaatgaagc cggcgcgcgg gtctttcagt ccggtacgga cgagttcggc
    14341 aagctcgcgc ataatttgtt ctttgacacg gtcttggcgg gcatagccgc gttggggttt
    14401 tctcatgaat atcctcagat ttcagatttg gtttcagacg gcatttttgc cgccggggcg
    14461 gttgcgaaag ggcgtattat agcctaatcg gcgggatttt aaggtaatac attgataaga
    14521 acggatatag ttattatact tggcaacccg aacggcacgt gccgttttct gtaacgcata
    14581 cattgtaaag aaagccatgt ccaacgaaaa ccgtacttgt tccttttgcg gaaaatccaa
    14641 atcacacgtc aaacatttaa ttgagggcga aaacgccttt atctgtgacg aatgcgtctc
    14701 aaactgtatt gaaatattgc atgaagacgg aaatgacggc acgccttcgg aaagtgccgg
    14761 aggggagccg gaagaatccg gcaagctgcc cacgcccgcc gaaatcgtcg ccaacctcaa
    14821 cgaccacgtt atcggtcagg aacaagccaa gaaagccttg gcggtgtcgg tgtacaacca
    14881 ttacaaacgc ctgcgccatc ctaaagcagg agctaatgtc gaattgtcga aatccaacat
    14941 cctgcttatc ggcccgaccg gatcgggtaa aaccctgctg gcgcaatctt tggcgcgcaa
    15001 actggatgtg ccgttcgtga tggcggatgc cacaactttg actgaagcag gttatgtcgg
    15061 cgaagacgtc gagcaaatca ttaccaagct tttgggcaaa tgtgatttcg atgtcgaaaa
    15121 agcccagcgc ggcattgtct atatcgacga aatcgataaa atttcacgca aaagcgacaa
    15181 cccgtcgatt acccgcgacg tatccggcga aggtgtgcaa caagccttgt tgaaactgat
    15241 tgaaggtaca gtggccagcg ttccgcccca aggcggccgc aagcatccga atcaggaatt
    15301 tatcaacgtc gataccacca acatcctgtt tatctgcggc ggcgcgtttg caggcttgga
    15361 aaaagtcatc cgccagcgta ctgaaaaagg cggtatcggt ttcggtgcgt ccgttcacag
    15421 caaggacgaa aatgccgaca ttaccaagct gttcggcatc gtcgaaccgg aagacttgat
    15481 taaattcggt ctgattccag aattaatcgg acgtttgcct gtgattgcaa ctttagaaga
    15541 attggacgaa gacgcgctga ttaatatttt gaccgagccg aaaaatgctt tggtcaagca
    15601 gtatcaggcg ttgttcggta tggaaaatgt cgaactggag tttgaagaag gcgcattgcg
    15661 ttccatcgcg cgtcaggcaa tggaacgcaa aaccggcgcg cgcggcctga ggtccatcgt
    15721 cgaacgctgc ctgctcgata cgatgtaccg cctgcctgat ttgaaaggct tgaaaaaagt
    15781 ggtggtcggt aaggcagtca tcgaagaggg cagggaaccg gaattggtgt tcgagtcctg
    15841 atggcaaaca aaaccgcttc ggaacgttga accacgtttc tgaagcggtt ttttgtttct
    15901 gatgccgtct gaagccttcc cgccttcaga cggcatttga gttttacgga ttgtctgatg
    15961 tcgcggcaaa ttccgtcagc gcgtgttcca aacgtcgcaa accttccgcc atatcctcat
    16021 cgttcaacag tagcgaaggc gcgaaacgca atacgtccgc acccgcaacc aaaatcatca
    16081 cgccgtgttt caaggcggcg gcggtgattt cggatgcgcg tccgcgatag gcttcgtcca
    16141 acacgcagcc gagtagcagc cccatcccgc gaacttgtga gaacaagccc gttttcctgc
    16201 acaaatccag caatgccgtc tgaagtttct gcccctgttc acggacatgg tttaaagttt
    16261 cgggcgtatt gatgatgtcg aatgcgcggc tgccgaccgc acacgccatc gggttgccgc
    16321 cgaaagtcga gccgtgcgtg cccggttgga aggcggcggc aatcttttct gtcgccagca
    16381 tcgcgccgat cggaaagccg cagcccaagg cttttgccga actcaaaata tcgggaacaa
    16441 tgccgtaatg ttcataggca aacagcctgc ccgtatgccc catcccggtt tgaacttcgt
    16501 ccaaaatcaa caacgcattg tgccggtcgc acagacggcg cgcggtttgc aaatattccg
    16561 cagtggcggg caggatgccg ctttcgccct gtatcggctc gatgatgacc gcgcaggtct
    16621 gttcgccgac ggcagcttcc agcgcggcaa tatcgttgaa cggaacgtgc gtaatgcctt
    16681 gcggcagggg tgcataatcc ttgctgtatt tcggctgacc gccgacggac acggtaaaca
    16741 gcgtgcgtcc gtgaaaactg ttgatacagg cgacgatttc gctttttcct ccgccgaaac
    16801 ggtcgcgggc gtatttcctc gccagcttca acgccgcctc attcgcttcc gagcccgagt
    16861 tgcagaaaaa aaccttgtcg gcaaaactgt ttgcaaccaa tttttgcgcc aattcctgcg
    16921 ctggacgcgt cgtatagata ttggaaatgt gccacagctt gtgcatctgc gcgtttaaag
    16981 catcgacaag ggcagggtgg cagtgtccca gcgcattgac ggcgataccg cctgaaaaat
    17041 caatatattc acgccctttc gtatcccaaa cgcggctgcc tgaagcgcgt tcgggaatca
    17101 tcggggcaaa ggcgaaattg ggggtcagat agttttgcat aattgctcct gattgcggtt
    17161 ttgctcggga ttatgcaccg tttttcaaag cagataaagg gaaattgccg acaatatcgg
    17221 gcagcggtaa aaataaaccg aaatattttc aatcggatag atgaaaaatc aaaaaaattt
    17281 cgataaaatg cttgaggcgc ggcagcgttt tgatttagaa tctccccaat atttttatct
    17341 tcagacggca tttcatcatg caaggcaaaa aatttacaac cgcttattgc tattggtacc
    17401 acacagacgt gcggtacttt tttgcgcttg cttgaaatcc atcccatacg caaaacaggc
    17461 cgctcgaaaa cggggcggct tttttgttac cttcacacag gtcgcccgat tcggcaacac
    17521 acgacagaat caggaaaatg accatgaatg caatgcccca aaccgccgca caaaacgaaa
    17581 ccctgcacac ttgtagcatt attgtcgatg atgcacagga tttttccccc atgcttctcg
    17641 atgcagggcg ggacgacaca cttatcagcg aatcgatgat tccgcaaatc cgcaccgttg
    17701 ccgccctgat agccgccgaa cggcacgact tcagccgcag cagccccgcc gagtttaccg
    17761 atgccgccga tttttttgcc gcacggattc tcgtattggg cgtgcgacgc ttccatctgg
    17821 acgtatcgct gcttcagatt ttgaaaaccg ccaacagccg cgcccgccgc tttgccgaaa
    17881 aacaccgcct gtttttcaca cccgcacaag ccgaattgag cctgaacaaa cacaaaaacc
    17941 gccgcctgct gaccgtagaa accgaacacg aagtcgaaaa caaaggcaac ccagtcgcca
    18001 acagcctcgc atttgtccgc aaattgcaca ccctgcccct gtaaacatca cgatttgccg
    18061 gcagaatgct tgccaaaatt cccgacggcg atacaataaa accgattttc cacccaaaca
    18121 ggacacacat gaaaaaaatc atcgcctccg cgcttatcgc aacattcgca ctcgccgcct
    18181 gccaagacga cacgcaggcg cggctcgaac agcagcagaa acagattgaa gccctgcaac
    18241 agcagctcgc acagcaggca gacgatacgg tttaccaact gactcccgaa gcagtcaaag
    18301 acaccattcc tgccgaagca caggcaaacg gcaacaacgg gcaacccgtt accggtaaag
    18361 acgggcagca gtatatttac gaccaatcga caggaagctg gctgctgcaa agcctggtcg
    18421 gcgcggcggc aggcgcgttt atcggcaacg cgctggcaaa caaattcaca cgggcaggca
    18481 accaagacag tcccgtcgcc cggcgcgcgc gtgcagccta ccatcagtcc gcacgcccca
    18541 atgcgcgcac cagcagggat ttgaacacgc gcagcctccg tgcaaaacaa caggcggcgc
    18601 aggcgcagcg ttaccgcccg acaacgcgcc cgcccgccaa ttaccgccgc cccgctatgc
    18661 gcggtttcgg caggaggcgg taaacccggc gcgccaatgc cgtctgaaga gctttcagac
    18721 ggcatttttg catttgttag ggacattgtt atgttgccgt ttgattttca gacggcattt
    18781 tgtttccaag cgtttgatgt cgggatggca attctgattt acgtgcgttg gcagggtggc
    18841 attgtcatgc cgccgttggg ttttcagacg gcatcatatc gaacagtcaa gccgtagggc
    18901 gagcattctt gcccaccgtt gcaagggcgg gattgggtgg gcaggaatgc ccaccctgca
    18961 tctgaaaccc ttccgtgtca ttcccgtgca ggaaggaatc tagatatttg aatttacgga
    19021 aattttcaat cgttgccaaa gcggaaaagg gtttttatta cagcctaatc ctttgggtat
    19081 caaagccggg cggtgctgaa acattgcaaa aatgccgtct gaaactcgaa atcggtttca
    19141 gacggcattg gtttatgcgg caggttgggg cttagcggtc tttgtaacgg cgttcgcctt
    19201 tgcctgcaaa agaatcgccg gctttgcgtt tgcctttgaa gccttcgccg gtttttttga
    19261 aaccgtctct cttaaagcct tctttcttga aaccttcgcc gcgcgttttg ccgccgaagc
    19321 cttctttgct cggtttatga tcgccgcgcc aaccgccgga tttacgatcg ccccagccgc
    19381 ctttgccttt cggcttgccg cctgcggatt tgcgtttgcg ggtcggttcc atgccttcga
    19441 tggtcagttc gggcagtttt cggttaatgt atttttcgat tttgtggact ttgacgtatt
    19501 cgttcacttc ggcaaacgta atcgcaatac ccgtgcggcc tgcgcggccg gtgcgcccga
    19561 tgcggtggac gtagtcttcc gcctgtttcg gcaggtcgta gttgataacg tgggtaatgg
    19621 tcggtacgtc gataccgcgt gcggcaacat cggtggcaac caaaattttg cagcggcctt
    19681 tacgcaaatc catcagcgtg cggttgcgcc agccttgcgg catatcgccg tgcaggcagt
    19741 ttgcggcgaa acctttttcg tacagttcat ccgcaatgac ttcggtcatg gctttggtgg
    19801 acgtgaaaat cacgcattga tcgatattgg catcgcgcaa gatatgatcg agcaggcggt
    19861 ttttgtggcg catatcgtcg cagtacagca gttgttcttc gattttgcct tgatcgtcca
    19921 cgcgttcgac ttcgatgatt tcagggtctt tggtcagttt gcgcgccagt ttgccgaccg
    19981 cgccgtccca agtggcggag aacaacaaag tctgacggtc gctcggcgtt gcttccacga
    20041 tggtttcgat gtcgtcgata aagcccatat ccaacatacg gtcggcttcg tccaaaatca
    20101 gcacttccaa acgttcaaaa tcaactttgc cgctttgcat caggtccatc agacggcccg
    20161 gcgtggcgac aatcagatcg accggtttgc tcagggcacg ggtttggtag ccgaaagacg
    20221 cgccgccgac gatgctgacg gtgcggaacc aacgcatatt tttggcatac gccagcgcgt
    20281 ttttctcgac ttgagccgcc agttcgcggg tcggggtcaa caccaaagca cgcgggcctt
    20341 tgcccggttt ttcgctgcgt ttggtcagtt tttgcaaagt cggtaacaga aaggcggcgg
    20401 ttttgccgga gccggtttgc gccgaagcca tgatgtcgcg gccttccaaa gcaaacggaa
    20461 tggcttgcgc ctgaatcggc gtcgggcttt cgtaaccctc gctgctgacg gcggacaaaa
    20521 tgtttttatc aaggttcaaa tcggcaaatt taatagacat ggctatcctt ggaagacaac
    20581 aacgcccgcg tccgaaaggt tcagacggcc tgaagcaggg aagggtaacg atacggggaa
    20641 tgtgaagtta cagggttgtc gcaagcggct tgcttgtggt ttacatcgac ggcaacctgc
    20701 acagatatgc tttgcaaaat caaagcaaca gaagaaacgc gcccgtaagg gcggtttagg
    20761 ttggaaggcg atggcttcgt atgacaagat aagcgacgga tggtataggg tttgcccgaa
    20821 acggtcaagc aatcgggcgt taattacagt aaaattgccg ttttccactt tattccgcct
    20881 gaaaccaatg accgacatca caccgtttgc caaccgcttg ggcaaaaaca tcaaacacct
    20941 tatgaaatgg gcgaaacgca acggcatcga agcctggcgc atttacgacc gcgacatccc
    21001 ccaattcccc tttgccgccg atgtttacgg cgatcgaatc caccttcagg aatacgatac
    21061 cggctggctg atgcggcccg aagaatacga agcgtggctt gccgaagtct tggaagccgt
    21121 cgctttcgtt accggttttg cgcccgaaca aatccgcctc aaacgccgcg aacgccaaaa
    21181 aggtttgcag cagtacgaga aaaccggcaa ggcgggcgac gatttcgtca tcaccgaaaa
    21241 cggccgcaag ttttgggtca accttgataa gtatttggat acggggcttt ttttggatca
    21301 tcgaaacacg cgcaaaaaag tcggcgaaac ggcggcgggc aaacgttttt taaacctgtt
    21361 ttcctacacc ggcagcttca ccgtctatgc cgccaccggc ggcgcggcat ccagcgaaac
    21421 cgtcgatttg tccaacacat atcttgattg ggcgaaacgc aattttgaat tgaacggcat
    21481 cgatacggaa cgacacaaaa tcgtccgcgc cgacgtgttc caatatcttc agacggcata
    21541 tggggaaggc aggcggttcg acctcattgt gatggacccg cccagttttt ccaacagcaa
    21601 gaaaatgtcc gacatcctcg acatccagcg cgaccacaaa aagctgattg acggcgcggt
    21661 gaagctgctc gcttcagacg gcattttgta cttttccaac aacctgcgca gcttcgtctt
    21721 agacgacttg gtatcggaac aatacgccgt caaagacatt tccaaacaat ccgttcccga
    21781 agatttccgc aacaaaaaaa tccataggtg ctgggaaatc cgccacaaat cttaagggtg
    21841 cgaaaaagcc gccggaatct tccggcggct ttttcgattg cgctttatca ggcgatagag
    21901 gcttcgacaa aggcggtcag ctgacctttt gccaatgcgc cgactttggt ggcgacgact
    21961 tcgccgtttt tgaacaccat cagggtcgga atgccgcgca cgccgaaacg ggacggggtg
    22021 gcttcgttgt cgtcgatgtt gattttgacc actttcagac ggccttcaaa ttcggcggca
    22081 atgtcgtcca aaatcggggc aatcattttg caggggccgc accacggagc ccaaaagtcc
    22141 agcaggacgg ggatatctgc atttaaaacg tctttttcaa aagcggcatc gctggtgtgt
    22201 acaatcaatt cgctgctcat tatttttcct tttcggttgg gattggcacg atagcgtgca
    22261 ggatagggct tgggcataaa aatttcaagt gggcgggcgg gataatagtt tttctgtacc
    22321 gccccaatcg cctttgcaaa ttctaaaaaa cggttcggac agtattttat ttctgaaaat
    22381 tacttttatt atcaaaatgt aaggaatacg tttgaggctg tacagaagcg cgcgggcggt
    22441 gtatgctccg tttgaattga ttcttaaagt atgaatgttc aaaaccaaac ccgaaaccct
    22501 gtaaaaggaa cactgcgacg actgattttt ccgtttggga gaccgccccc tttggtgcaa
    22561 ccgtcgatca tatcctgcaa cgctaccaca atgtccaccg tgcacaattt gaagaattgg
    22621 tgccgctggc gcaaaaagtg gcccaagttc atgccgacac ctttccggcg gaaattgccg
    22681 agctgcttgc ctatatgcag aacgaactgc tgatgcatat gatgaaagag gaaaggatgc
    22741 tgtttccgat gattaatcag ggtgtcggcc ggggtgcgac gatgcccatc agcgtgatga
    22801 tgcacgaaca tgaagaacac gaccgcgcca tcgcacggct caaagagctg accgataatt
    22861 tccaagcgcc cgaaggagcc tgcggaagct ggacacggct ttacgttttg accaaagaaa
    22921 tggtggacga cttgaacgac cacattcatt tggaaaacga tattctgttt gcccgcgtac
    22981 tcgattctta aggaacggga gccggatatt tccatgccgt ctgaaatacg gtttttccgc
    23041 ttcagacggc atttccacgt ttcagcccaa caattcgggc ggaatgcggc acacgggaat
    23101 cgggttgatt ttgtgctgca tcgcgcggtg cagccgcgta tagatttcca gaacttcgcg
    23161 ctgccgccct tcaaaatctt cgggtttgcg cgtgccgtac acgcccattg cccactccag
    23221 ttcgggatag cttgcgccca tctgttcttc gtcggtgcgt tccgtatccc acaggccgtc
    23281 ggtcggcggg gctttttgaa tcgcctcgtc cacgcccaat gcttcggcaa gccggtaaac
    23341 ctgcgttttg gtcaggtcgg caatcgggct gatgtccacg ccgccgtcgc cgtatttagt
    23401 aaaaaagccc acgccgaaat cttcaatctt attacctgtc cccgtaacca gcagtccgtg
    23461 tatctgcccg tagtagtaca gggtcagcat acgcaggcgg cttctggcgt tggcgaggga
    23521 aagcggctga ttggcaaatg ccgtctgatg agtgccgacg gtttgttcaa aggtctggaa
    23581 ggtgtcggtc agatcgaccg tttgcgcgct tacattggca tattgccgtt gcagattgcg
    23641 gatgtgcagc cttgcccgct caagctggcc ggggtgttgg cgtatcggca tatccagaag
    23701 cagcgtgggg cggccggtgc gggcggcgag tgcggagacg acggcggaat cgatgccgcc
    23761 ggaaacgccc acgacaaacc cttttgcatt tgcccgggcg gcgtattcgt ccaaccatcg
    23821 ggcgatatgt gtgataactg cctgcgtatc catttttcct cctgtcgaaa atatccgcat
    23881 catagcctgc cggcggtgca atttgaatag attgcaggca ggttgatgaa tatttttccc
    23941 attccccgct taaaaaagtc atacgggaaa aggcgggcgg caagatggcg cgtgttataa
    24001 tattgttgac aatttaggcg gcccggtgcc gccttaggct tcaaaacata ttcatatttt
    24061 ttactatttg attgattcga tgtctgattt ttttcattct gacgtgttgt ccgtttccga
    24121 gttgaacgca tttgccaaaa gtcttttgga aaaccacctt gccggtttgt ggattgccgg
    24181 cgaagtatcc aacctgaccc gtgccgccag cgggcattat tatttctcgc tcaaagacag
    24241 ccgcgcacag gtgcgttgcg cgatgttcaa gggtgcggcg gtgcgtttgg cgaagccttt
    24301 gaaagaaggc gaccatatcg aggtatcagg aaaaatcagt atttatgaag cgcggggcga
    24361 atttcagatt accgtaaacg aagtacggct caaaggtttg gggcagcttt acgaagccta
    24421 tgagcggttg aaggcgcagt tgcaggcgga aggcgcgttt gcggcggaac gcaagaaacc
    24481 tttgcccgtc cgtccgcaat gtatcggcat cgtaaccagt ctggcggcgg cggctttgcg
    24541 cgatgtcgtt actaccttga aacgccgcgc acccgaaatc cccgttatcg tttatccgac
    24601 acccgttcaa ggcgcaggca gcgaattgca aattgcccaa gcgattaaaa ccgcctcgca
    24661 acgcgccgaa tgcgatgtgt tgattgtctg tcgcggcggc ggcagcattg aagacttgtg
    24721 ggcgtttaac gaagaaccgg tcgtgcgcgc catcgaatcc tgcacggttc cggtcgtcag
    24781 cggcgtaggc cacgaaaccg atttcacgct cgccgatttc gtcgccgata tgcgcgcgcc
    24841 cacgccgacc ggtgcggcgg aactggtcag ccccaaccgc caagaatcgc tgcaccgcct
    24901 cgcccaagcc caatgccgtc tgaaaaccgt tttggaacaa cgctatttcg atgccagcca
    24961 aaaactcgac tggctggcgc ggcaaatccg tcatccgcgc caaaaactcg acgaacagcg
    25021 cgcttcaatc ggcaagctgg cgcaaacgct gtcttactcg atgacgcaaa acgtccgtac
    25081 ccacaccgcc cgtttcgaac gccaaaccca agccctgaaa cattgccgtc ctgatgtttc
    25141 cgtttgcaaa aaaaacatcg accgctttca gacggccctg tcgcacgcct tccaccaact
    25201 gcttacccac cgccgccaaa gcctgactgc ccaagccgca ttgctcgaag ccgtttcgcc
    25261 gcagcacatt ctggagcgcg gcttttccgt cgtcaaaaac acacgcggac aagtcatccg
    25321 caacgccgat gttttgaagc aggggcagaa gctgcacatt atcttcgccg acggcgaaac
    25381 cgacgtgcgc gtaagcaaag aacaagggca gcaggatttg ttcgattgca tttaaaccca
    25441 atcggaaaaa ccgctccccc gccgccgtca ttcccgcgaa agcgggaatc cagtcccttg
    25501 accttaagat aatctttaat tcgtcattcc tgaaaaagcg ggaatccgga agcgaaaagc
    25561 cgcaggaatt tgtcggaaaa aacagaaacc tttccgtaca cccgaaatgt ttcagacggc
    25621 gcatcttggg gcggataaaa acccgacccg atattttttt ataattttca ataagtttcc
    25681 attgggcaaa tgaaatttta tttttcaact gacagaaaga ggatgttatg aagtttttag
    25741 accgtgaggc aagcattgcc aagccgggtt tcaaccgttg gctcgtaccg cccgcagcat
    25801 tggcggtaca ccttgccatc gggcagattt acgcctattc cgtattcaac gcgccgctga
    25861 ccaaacttat cggcataacc gaatcggcgg ccggagattg gaagctgacg accgtaggtt
    25921 ggattttcag tatcgcactc gcgatgctgg gcgcgtcggc cgccctgttc ggtacgtgga
    25981 tggaacgggt agggccgcgc aaagccatat ttgccgccgc ctgctgcttc agcctgggct
    26041 tcttcgtatc cgcattcggc gtgcgtacgc acaacctctt cctgctctat ttgggcaacg
    26101 gcgtcatcgg cggcgtaggc ttgggtttgg gctacatcgg gccggtttcc acactgatga
    26161 aatggtttcc cgacaagccg ggtatggcga ccggtttggc gattatgggt ttcggtggcg
    26221 gcgcaatgct ggcctcgccg ctttccgtat cactgatgaa caccttttca aacgctgctt
    26281 cggtcggggt tgccgaaacc ttcgccgtat tgggtctgct ttacctcgca ctgatgatgt
    26341 tcggcgcgtt taccatccgc gtacctgccg acggctggaa acctgaaggc tataccgcgc
    26401 cgaaaacgca aaacaagctg gtcagcagca atcatgtcaa cgtgtcccaa gccatgaaaa
    26461 cgccgcagtt ttggctgttg ttctgggtat tgtgcctgaa cgtaactgcc ggcatcggcg
    26521 tattgggtca ggcatccgtg atgattcagg aactcttttc cgaaacctcc ataggcaggc
    26581 aggcagcagt cggcgcaggt gcggcggcag gcttcgtcag cctcctgagc ctgtttaaca
    26641 tgggcggacg ctttttgtgg tcgagcgttt ccgacaaaat cggacgcaaa aacacctaca
    26701 ccatcttctt cgtactcggt tcgctgctgt atttcgccgt tccctccatc ggcgagggcg
    26761 gcagcaaagc cctgttcatc atcggctttt gcgtgattat ttccatgtac ggcggcggat
    26821 ttgccgccat tcccgcctac ctgaaagacc tgttcggcac ctatcaggtc ggcgcgattc
    26881 acggacgcat cctgctggcc tggtcaaccg ccgccgtcat cggcccggta ctggtcaatt
    26941 acatacgcca aagccaaatc gacagcggca taccggcggc acaggcatac agcgtaacca
    27001 tgtacatcat ggcgtgcctg ctgattatcg gtttgttgtg caacctcgcc gtcaaatccg
    27061 tacacgaaaa acaccacgaa aaagacatca aaaccgccgc acacagcggc aaccccgatg
    27121 acgaaaccgc catatccgac gcttaccttg tgggcgaaaa agtttccggc ggcggcatat
    27181 cggtctggtg gcgttgggca ttggccgtca taccgctcgc ctacggcgtg gtgatggtct
    27241 ttgtcaaagc actcgacctt ttctcctgaa aacgcccgaa taaaatgccg tctgaaacag
    27301 cagatgtttc agacggcatt tttgtgtttt ggcgggttta ggcttttttg cggcggcgtt
    27361 cgcccggcag cagcatgtcc gcccatttga tgatgttggc gacttcggcg gggttgagtt
    27421 cgtagaactg cccgcgtttg aggcggttgg gcagtccgat gggaccgaag ccgatgcgca
    27481 cgaggcggct gacggtgagt ccttggcttt caaaaatgcg gcgcacttcg cggttgcggc
    27541 cttctttaat cacgacgttg taccatttgt tcgcgccttc gccgccttgt tcgcggatgc
    27601 gttcgacttt tgccaagccg tcttcgagca tcacgccttc ttcggtgagg acgcgcattt
    27661 gttcgccggt cagcccgccc aagacgcgca cggcgtattc gcgttcgact tcgaagctgg
    27721 ggtgggcgaa acgttggacg agttcgccgg aggtggtaag aatcagaagt ccgctggtgt
    27781 tgatgtccaa gcgtccgatg gcgacccagc ggctgctggc ggcctgcggc aggcggtcga
    27841 atatgctgac gcggccttgc gggtcgtcac gggaaacgat ttcgccttct tgtttgtaat
    27901 acaggatgat gcgcggcagg cggtccgccc atttgagctt gatgatgctg cctttgacgg
    27961 taacgtggtc gtcgggggta actttgtcgc ccagttgcgc ggttttgccg ttgaccgtta
    28021 tccagccgtt ggtaatccat tcttccattt cgcggcgcga gccgacgccg gacgcggcaa
    28081 gcactttttg caggcgttcg ggttccatgc gcgacaggtc gctgcggcgt tctttcaaat
    28141 cgcgcgcgtg ttccataatt ttttggttgg gattgcggac gacgagtttt ttggctttgg
    28201 cggcgcgctg tttggggacg ttttgaggtt tgatgccgtc tgaagctttt tgtccgtaag
    28261 gttgggaagc ggttttgccc gtttcatctt tgggacgggc tttgcttttg aacggtttgg
    28321 cggttttctt ggcagacggg gctgcgccgt cgcgccattg gcgtttgctg gtgggctgtt
    28381 tggacatcat ttttcctaac tcgcctgata aggcggttct tctgcggcgt gtgcggcagg
    28441 acgggttgat aaaatgccgt cctcggtcgg acggttcgat attgtacctg atgcggcggt
    28501 tttgcggatg aaatcgtcgc tgcggcttgg ggtcggggca aagtttgtgc gacggttgaa
    28561 atcggtttgc gtgcgggagt agaatctttc aaatgataag aggagcagta aatggatttg
    28621 cacaatattc gggaagatta cagcaaacgg gaattgtcgg aagccgattg cgccgataat
    28681 ccgatcgagc agttcgagcg gtggttggac gaggcggtac gcgcacaggt caacgagccg
    28741 acggcggtca atgtggcggc ggttgacgga cgcggcaggc ccaacagccg tatggtgctg
    28801 ctgaaggaag ttaattctga aggttttgtt ttctttacga attatcacag ccgcaagggg
    28861 cgttcgctgg atgctcatcc atttgcggcg atgacgtttt tttggccgga gctggagcgt
    28921 caggtgcgcg tggaagggcg tgttgaaagg ctggcggaga agttgtcgga cgaatatttt
    28981 gagagtcgcc cgtatcagag ccgtttgggt gcgtgggcaa gtgcgcagag cgaggttatt
    29041 ccgaacaagg cggtattggt ggcaaaggcg gcggcggttg gactcaaaca tcctctgcat
    29101 gtgccgcgtc cgccgcattg gggcggctat attgtgattc ccgatttgct tgagttctgg
    29161 cagggcagac cgagcaggct gcacgaccgc atccaatacc gtttgctgga tggtggctgg
    29221 atacgcgagc ggctgtcgcc ttgaacagga aaaaacatat gccgtctgaa aaccggaatc
    29281 gggtttcaga cggcattttt tcacggggaa aacacggacg ggaagttttt tgtgttctga
    29341 acggaaagct cgaatcctct ttataccggg gaattatgat gcggacggcg tgaatgcgtc
    29401 ggagaaaaac aagttttccg gcagcttatg ctgttgcaca aacagattct ttgtttgttc
    29461 ggtcatggcc ggagaaccgc aggcaaatac ttcgtattcc gacaggtcgg ggtggtcttg
    29521 tgccgcgatg tcttgtacgt gaccatttct tccctgccag ccctctccgg ggcgggacaa
    29581 tacgggggtg aagcaggcgt ttttcagacg gcatgccaac ccttgtgctt cttcgagggc
    29641 atacaaatca tcctgatgac gcgcgcccca gtagaaatgg acggcgcggt tgctgccttg
    29701 gcggataagg tcgagcagga tgctgcggat gggggcgtag cctgtgccgg ttgccagcag
    29761 gatgacgggt ttgccgctgt cttcctgcaa ggtaaacgaa ccgagcgggc ctttaacgcg
    29821 gacgatgcct ttttctttga ctttgggttc gctgccgaaa atcatttccg agcagacacc
    29881 gttttcgtgc ctgcggatgt gcagttccaa aatgccttct tggtcgggta aattggcgat
    29941 ggagtagctg cggctgacgt tgcccggcag cagtaaatca atgtattgcc cggcgtagaa
    30001 ggcaaacggc ggggctttgg gcagggcaag tttcaggagg gcgacatcgt gtttgaaaat
    30061 aatactttcg atgcgtgcgg gcagggtgcg gacgggtagg gcatcggctt tgtagccggg
    30121 gatgttgatg ttgatatcgc tttgcgcagt ggtgcagcac atcaaaatct tgccttgcgc
    30181 tttttctgct tcggataaag cctgttccga gtgtccgccc atttgaatat cgccgctgac
    30241 cagttcggct ttgcattgtc cgcagacacc gcttttgcag gaatggggca ggttgaggtt
    30301 ttgacgggcg gcagcggtca aaacggtttc gccgtcgttg gcggcaaagg tggtttggtc
    30361 gggcagggta acggtgtggt tcatgtcttg gtatggtttg agcagatta
//
LOCUS       DAXHNE010000008        30138 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.008, whole genome shotgun sequence.
ACCESSION   DAXHNE010000008 DAXHNE010000000
VERSION     DAXHNE010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30138
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.008"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(93..932)
                     /locus_tag="ACFM4Z_000269"
     CDS             complement(93..932)
                     /locus_tag="ACFM4Z_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HFC6020373.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1217..1292
                     /locus_tag="ACFM4Z_000270"
     tRNA            1217..1292
                     /locus_tag="ACFM4Z_000270"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1250..1252,aa:Arg,seq:ccg)
     gene            complement(1357..2550)
                     /locus_tag="ACFM4Z_000271"
     CDS             complement(1357..2550)
                     /locus_tag="ACFM4Z_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFC6020374.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVRRAETARAAAPAPSPYLPMEGLDVYRSAVQHLLFGKGNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPAAVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2571..3659)
                     /gene="trmA"
                     /locus_tag="ACFM4Z_000272"
     CDS             complement(2571..3659)
                     /gene="trmA"
                     /locus_tag="ACFM4Z_000272"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFC6020375.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDNEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSVGAAQWNIEANGIGNIKIARLSAEEFTEAYTGKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLAETH
                     AVERAALFDQFPFTHHIESGVLLKKKIL"
     gene            3802..4902
                     /gene="aroC"
                     /locus_tag="ACFM4Z_000273"
     CDS             3802..4902
                     /gene="aroC"
                     /locus_tag="ACFM4Z_000273"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFC6020376.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSESDIQAD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIAQSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDIHVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVQVNTPDITLSNK"
     gene            4994..5419
                     /locus_tag="ACFM4Z_000274"
     CDS             4994..5419
                     /locus_tag="ACFM4Z_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFC6020377.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETLSVEAEAAESSAAE"
     gene            complement(5493..7478)
                     /gene="parE"
                     /locus_tag="ACFM4Z_000275"
     CDS             complement(5493..7478)
                     /gene="parE"
                     /locus_tag="ACFM4Z_000275"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFC6020378.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     ASDEALGGFATAIDVQIHEDGSLSVHDNGRGIPVGLHPEEGVSVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDEHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVATVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDSILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7545..8093)
                     /locus_tag="ACFM4Z_000276"
     CDS             complement(7545..8093)
                     /locus_tag="ACFM4Z_000276"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFC6020379.1"
                     /translation="MNLTEGDTVLDREGYRPNVGIILINNRNEVFWGKRVREHSWQFP
                     QGGIKPGESPETAMYRELYEEVGLLPQHVKIIGRTRDWLRYDVPNNWVRREWRGSYRG
                     QKQIWYLLRLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELS
                     SRFLRGMESYEDFAARQSSDNR"
     gene            8235..9530
                     /gene="serS"
                     /locus_tag="ACFM4Z_000277"
     CDS             8235..9530
                     /gene="serS"
                     /locus_tag="ACFM4Z_000277"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFC6020380.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDTLEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMNQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPAGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVADSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRLKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9608..10606)
                     /locus_tag="ACFM4Z_000278"
     CDS             complement(9608..10606)
                     /locus_tag="ACFM4Z_000278"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFC6020381.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEAEKIGGKYVDLDELYARSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10731..11807)
                     /gene="prfA"
                     /locus_tag="ACFM4Z_000279"
     CDS             complement(10731..11807)
                     /gene="prfA"
                     /locus_tag="ACFM4Z_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFC6020382.1"
                     /translation="MKPSILEKLQQLSDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGELDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PVDLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLEEITNALIAEHQAELLAAMGD"
     gene            complement(11912..12682)
                     /locus_tag="ACFM4Z_000280"
     CDS             complement(11912..12682)
                     /locus_tag="ACFM4Z_000280"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC6020383.1"
                     /translation="MNFTDTHCHLADPALRENLPHILTAARKVGVKRFIVPATRPQDW
                     QDVADLAEMPSEHGQIRIALGIHPWFSDGIAERDFVRLETMLAHYPQAWVGEIGLDFY
                     DKTQTSQQRERQIQVFVRQLEIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANIPGIAKIAAEIRGTCVEEIAKVTERNADALLGR"
     gene            complement(12690..13685)
                     /locus_tag="ACFM4Z_000281"
     CDS             complement(12690..13685)
                     /locus_tag="ACFM4Z_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFC6020384.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVSQALSPFSDGLDFE
                     WHVCNPLIDSSSVTLQHWRDWLDIIADKLPSCDGILILHGTDSMAYTANLLALALQGL
                     GKPIVLTGSQWPYAAENSDAPRNLSTAVAAFSLKLKQTVIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGTLAEWDETRGWHNLRLPSQDAAAVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAQALILQSYGHGNTPADEGFIRAVRDFARQGKLLLNISQVRQGK
                     TAAVYAQGNAFRNSAGIINGGKCNLETATALMTLAVSQGWGKEDVYKELVRLKLV"
     gene            complement(13967..14650)
                     /locus_tag="ACFM4Z_000282"
     CDS             complement(13967..14650)
                     /locus_tag="ACFM4Z_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC6020385.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLAGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFVILGIGATVVAWIWWKKRQRIQFYRSKLKEKRAQRKAAKA
                     AKKAAQSKQ"
     gene            complement(14839..16173)
                     /gene="glmM"
                     /locus_tag="ACFM4Z_000283"
     CDS             complement(14839..16173)
                     /gene="glmM"
                     /locus_tag="ACFM4Z_000283"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFC6020386.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVVDTANGAGYGVAPKVFHELGAQVVSIGDEPDGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRSWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIGKGQNWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIASAIGSL"
     gene            complement(16303..17154)
                     /gene="folP"
                     /locus_tag="ACFM4Z_000284"
     CDS             complement(16303..17154)
                     /gene="folP"
                     /locus_tag="ACFM4Z_000284"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFC6020387.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNARTA
                     LAHAERLLKEGADILDIGGESTRPGADYVSPEEEWARVEPVLAEVAGWGVPVSLDTRR
                     TVVMEKALALGGIDIINDVAALTDEGAVELLARQADTGICLMHMQGLPENMQINPKYQ
                     DVVGEVARYLKTRSETCVAAGIAPQRITLDPGFGFGKTLQHNIALMRHLPELMAETGF
                     PLLIGVSRKRMIGELTGEADAAERVHGSVAAALASVARGAQIVRVHDVKATADALKVW
                     EALGINL"
     gene            complement(17294..18187)
                     /locus_tag="ACFM4Z_000285"
     CDS             complement(17294..18187)
                     /locus_tag="ACFM4Z_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFC6020388.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            18278..20392
                     /locus_tag="ACFM4Z_000286"
     CDS             18278..20392
                     /locus_tag="ACFM4Z_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFC6020389.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDQTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDNPKRKASVNRIIIENSTVRLDF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSNGIGPPEISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTAGGEYAQWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(20456..21934)
                     /gene="ubiD"
                     /locus_tag="ACFM4Z_000287"
     CDS             complement(20456..21934)
                     /gene="ubiD"
                     /locus_tag="ACFM4Z_000287"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFC6020390.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRIAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPVKPDGTRYGYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGENVDLYKLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWGELGL"
     gene            complement(21954..22469)
                     /locus_tag="ACFM4Z_000288"
     CDS             complement(21954..22469)
                     /locus_tag="ACFM4Z_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HFC6020391.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPDPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLPIPIIGHEETLEYGVWVSVSEKSFNDYQSHFHDNPENAVYF
                     GMICNRLPPYETDTFGLHCNVVTQPDGQRPLLQLHQGSEHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22476..22955)
                     /locus_tag="ACFM4Z_000289"
     CDS             complement(22476..22955)
                     /locus_tag="ACFM4Z_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020392.1"
                     /translation="MYGREAGWFVWLAAAMCGLWLLRALTAKLPQQRYAALFITLFFA
                     AVLVFGRHDSMYWYPVLVNLMMLAVFGGSLFAGQTVIEKLARLQQPDLPEKAVRYTRR
                     VTQVWCVFFIINGTLAALLARLGRYDWWAVYTGVIAYVLMGMLFAGEWLYRKLVLKV"
     gene            complement(23190..23435)
                     /locus_tag="ACFM4Z_000290"
     CDS             complement(23190..23435)
                     /locus_tag="ACFM4Z_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFC6020393.1"
                     /translation="MTEQEIYRLLRDTLTELFEIEPERITPDTNLYEDLEIDSIDAID
                     LIDRIKRETGRKLQAEDFRNVRTVNDVVQAVLKIQAG"
     gene            complement(23447..23707)
                     /locus_tag="ACFM4Z_000291"
     CDS             complement(23447..23707)
                     /locus_tag="ACFM4Z_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFC6020394.1"
                     /translation="MNDLENQIKQLIIDSLALEDITAADIGSEDALFGDGGLGLDSVD
                     ALELGLAVQKHFGFRLDGEQENLREHFANVKTLAAFVKSRQA"
     gene            complement(23704..24462)
                     /locus_tag="ACFM4Z_000292"
     CDS             complement(23704..24462)
                     /locus_tag="ACFM4Z_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC6020395.1"
                     /translation="MDKLDYCRRFFATWLGFVIFGVGGIMMKLVLLPYTLNGTSGSVA
                     RQLAARRIIGTSWRLFVAYLKWSGVLEVSFKGVEKLNRPGQLILANHPSLLDVVLLVG
                     HVPKMNCIVKKDLQHNPAMSSQIKGAGYIPNEESEAMLETVKAVFDSGQSLLVFPEGT
                     RTGWDGRVKMHRGAVSLGLRYAEVITPVCIKMNPPNFKKGQPWYRIPPKRIRYEITVG
                     DDILPQDWLAEKPLPIAARRLNEYLQDYFTRKTI"
     gene            complement(24455..25210)
                     /locus_tag="ACFM4Z_000293"
     CDS             complement(24455..25210)
                     /locus_tag="ACFM4Z_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFC6020396.1"
                     /translation="MPSIICRFHFDIAAWRVSGSKMRDMAQWAKWAECPDFADGLPDV
                     RPELPFLPAMQRRRLSKAARLVCDAAWDIASAHPGSPVVYASHDGEMARSFDLWLELL
                     KSHTVSPTSFGLSVHNATAGQWSILRQDMSEQTALAVCADGVETALAEAASLLEEGCG
                     SVLVLAADDPLPEGYAVSATRAPFAYALAMVLTKGTRYSLTLSASDDMPSEAGMLPEA
                     YWSGLEWVRFLLNGSRECRRVYRNREWLWQRNG"
     gene            25365..25898
                     /locus_tag="ACFM4Z_000294"
     CDS             25365..25898
                     /locus_tag="ACFM4Z_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog family protein"
                     /protein_id="HFC6020397.1"
                     /translation="MPVFASLPPQYMDTLLECPITNTASLLPHSGRMVLIDRITRYGD
                     DFVEAGAQVSPNHILLLDDKLPYTAFIELMAQAVGAYAGIQARKNARSVRLGFLLGTR
                     KLEIFAQSVPIGTHLLATAHMSIQDAGGMGVFDCELRWTDAPETSSETLPSDGILARA
                     SLNVYSPEHPAGTTDAV"
     gene            25926..26654
                     /gene="fabG"
                     /locus_tag="ACFM4Z_000295"
     CDS             25926..26654
                     /gene="fabG"
                     /locus_tag="ACFM4Z_000295"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFC6020398.1"
                     /translation="MTETVLITGSNRGIGKAVAFGLAEDGFDIAVHCRSRRDEAEAVA
                     EEIRALGRNARVLQFDVSDREACREILTADIEANGAYYGVVLNAGLTRDNTFPAFSDD
                     DWDVVLRTNLDGFYNVLHPLVMPMIRRRKAGRIVCMASVSGLTGNRGQVNYSASKAGI
                     IGAAKALAVELAKRKITVNCVAPGLIDTDIIDENVPVEEILKAVPAALMGLPEEVAHA
                     VRFLMDEKAAYITRQVIAVNGGLC"
     gene            26648..27898
                     /locus_tag="ACFM4Z_000296"
     CDS             26648..27898
                     /locus_tag="ACFM4Z_000296"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFC6020399.1"
                     /translation="MLNTRRVAVTGIGGITAFGRDWQSIQAAFKAEKNAVKYMDWHER
                     FPELEAQLGAPIENYAPPKHWTRKQLRSMGRVSYLCVDAAEQALADAGLLGDESITDG
                     RMGVACGSSSGSTKDIGDVGELLLTGTSRNFSANTYVRMMPHTTAANIGIFFGLKGRI
                     IPTSSACSSGSQGIGYAYEAIKYGLTDMMLAGGGEEFFPSEVYVFDSLYAASRRNGEP
                     EKTPRPYDANRDGLVIGEGAGIFVLEELEHAKRRGAIIYAELVGYGANSDAYHISTPR
                     PDAQGAILAFQTALQHANLAPEDIGWINLHGTGTHHNDNMESRAVAAVFGNNTPCTST
                     KPQTGHTLGAADAIEAAFAWGIADRQSNPEGKLPPRLWDGQNDPNLPAINLTGSGSRW
                     ETEKRITASSSFAFGGSNCVLIIG"
     gene            27952..28027
                     /locus_tag="ACFM4Z_000297"
     tRNA            27952..28027
                     /locus_tag="ACFM4Z_000297"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27985..27987,aa:Ile,seq:cat)
     gene            28102..28860
                     /locus_tag="ACFM4Z_000298"
     CDS             28102..28860
                     /locus_tag="ACFM4Z_000298"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6020400.1"
                     /translation="MKKVSVLIVAKNEANHIQECIESCRFDKEVIVIDDYSTDNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQPPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            28861..29925
                     /locus_tag="ACFM4Z_000299"
     CDS             28861..29925
                     /locus_tag="ACFM4Z_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC6020401.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRDCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAGH
                     QCVAARFTINHTADKLLDAI"
BASE COUNT         6989 a         8509 c         7796 g         6844 t
ORIGIN      
        1 tcctgatttt tgttaatcca ctataaattc ccataaaaaa acggagcaga tacctgcccc
       61 gtttttattt aatccgaaat tttaatctaa atttagaatt ttgcaccgga ttggtttgcc
      121 atatagtcaa cagccgcttt gacttcgtca tcgctcaaac ctgcattgcc gcctttggca
      181 ggcatcgcgt taaagccttc aagggcgtgt ttgtgcaagg tttctttgcc ttttttgata
      241 cgcggtgccc aatcgtcttt tttgcctatg ccgggaatac cgggaatcga accgccgtgg
      301 cacacctgac aggttgcttc gaagactttt ttaccgtcaa cgccgaccgc aggggctgcc
      361 gcacccttgt cttctgcctt cgcttctgcc ggagctgcac tatcggcagg agcagaagct
      421 gttcctgaag cggcattgtc ggcaggcgca gcctcatcag gattcgggaa agaaccgccg
      481 cttttgttcg ccatgtaagt aatcgcccgt ttgagttcct gatcggtcag gtctgccgca
      541 ccgcctttgg caggcatggc gttaaagccg ttcagcgcgt gttggaacaa ggtatcgaag
      601 ccttgcgcga tacgcggtgc ccaatcgccg ttgtgttcca gtttcggagc gttcggcaca
      661 ttgctgtccg ccgcgtggca ttggatacag attttgccga aaatctgttc gccttggcgt
      721 tcgccgacgg ggatgccgtc gcccatcgtc aattgtccga caggctggat acgggtctgc
      781 gttgctgctt ccgtagtggc atcgacatcg ccgaacgagc cgctgcccgc cagcttaatc
      841 aggaaataaa ggactgcaat aacaataacg ataccgctca caagggtaaa cagtgcagag
      901 ccttgggctt tattgtcgcg gagttgtttc atttggtagg cctcgccgtc aggttaggtt
      961 gtgctgtaaa ttatagtttg gtgtgttaaa cgcagttaac aatattttgc tggattatac
     1021 tgaattcaca gggtctttcc aatcgctatc attgaaaata tgaaaaaatt tgccaacggt
     1081 atctgtataa aacaaacaat cctttgaaaa taattgttta tcctcaagaa aactctcctt
     1141 atgccgccat acgccgcctg ccggcgcaag ataacctttg ccaatttgca gaatttacgt
     1201 taaccttacg ttttacgcac ccatagctca gttggaagag tgtcagtttc cgaagctgga
     1261 ggtcacaggt tcgatccctg ttgggtgcgc caattataaa gagaccgtct gaaagataaa
     1321 tatttttcag acggtctttt gacttacttc aaactcttat ttcaagactt ccgcaaatgc
     1381 gcgggcaaca tagtcggtat tcgacgtatt cagtccggcg acgcacatcc tgccggaatc
     1441 cagcaggtaa acggcaaatt cgtcgcgcag cctgcggact tgttccacgc tcaatcctgt
     1501 gtagccgaac atgccgcgct gtttgatgaa ataagtgaaa tcgcgatcgg ggatttgcgc
     1561 agttaagaca tcataaagtt tctgccgcat cgcacggatg cggtcgcgca tcatataaac
     1621 ctcgttttgc cacaaggcgt aaagttcggg gctgttcatc acgtcggcgg cgatatacgc
     1681 gccgtgcgcg ggcgggctgg agtagatgcg gcggacggtg aatttgagtt gtccgaacac
     1741 caaatccgct tcttccttat tcgggcaaac cacgctcagg ccgccgacgc gctcgccgta
     1801 gagcgacagg ttttttgaga aggaattgct gacgaacaag ggcaattcca tttccaccgc
     1861 tttgcggacg gcgtaggcat cgctgtccaa atcgccgccg aagccttggt aggcaatgtc
     1921 cataaacgga atcagtttgc gcgttttgat gatgtgcaac acttcgtccc attgttgttc
     1981 cgacatatcc acgccggtcg ggttgtggca gcagggatgg aggatcagga cgctgttttc
     2041 aggcagggtg ttgaaaaacg cggtcatttc gtcgaatttc acgccgacgg cagcagggtc
     2101 gtaatatggg taagtgccga cctcgaaacc tgcaccttca aaaatgccgc gatggttgtc
     2161 ccaagtcggg tcgctgacgt aggcgcgcgc ttcaggaaac cagcggtgca ggaaatctgc
     2221 tccgactttg agcgcgcccg agccgcccaa ggtttgtacg gtaacgatgc gcccttgcgc
     2281 gagcgcgggg ttgcctttgc cgaacaataa atgctgcacc gcgctgcggt aaacgtccaa
     2341 gccttccatc ggcagatagg gcgacggcgc aggcgcggcg gcacgggcag tttcggcgcg
     2401 gcgcacggat tccaatacgg gcatcttgcc ttcgtcgtca aaataaatgc ctatgcttaa
     2461 attgactttt tcggggcgcg ggtcgttttt gaaggtttcg accaaactca aaatcgggtc
     2521 gccgggatag tattcgatgt gtcggtacat agtccttacc tcttgctttt tcaaaggatt
     2581 ttctttttca acaatacacc actttcgata tggtgcgtaa acgggaattg gtcgaacagg
     2641 gcggcacgtt cgaccgcatg ggtttccgcc aaggtgtcca aattggcgcg caacgtttcg
     2701 ggattgcagg aaatgtagat gatgttgtca aactgcgaca ccagcttcaa agtttcctca
     2761 tcgataccgg cacgcggcgg atcgacgaaa atagtggaaa atgcgtaatc cgtcaaagca
     2821 ataccgccat ccttaaggcg tttaaactca cgtttgccgg tataggcttc ggtaaattcc
     2881 tcggcagaca gacgggcgat tttgatgttg cctatcccgt ttgcctcaat attccattgc
     2941 gctgcgccta ctgaagtttt ggaaatctcg gttgccaaaa cacgttcaaa cttttcagat
     3001 aaaggaagcg taaagttgcc gtttccgcaa tacagttcga gcaaatcccc atccaatcct
     3061 tctgctgcac cgcaggccca ttcaagcatt ttctgacaca cggcggcatt cggttgggta
     3121 aaactgcctt caatttgccg ataacggaaa tcccggttgc cgaccttcaa agtttccgtt
     3181 acatagtcct gttttaagac tattttctgt cccctgctcc gcccgatgac ggaaatatcc
     3241 aactgttgct gtaacgcttg cgccgcctgc atccactcat tatcaagcct tttgtggtaa
     3301 atcatggtaa ccagcatttc cccgctgagc gtagacaaaa attcgacggc ataccaacgg
     3361 tttctcagtt cggcagattg ggcggcaacg gcaatcaatt ctggcatcag gcggttgacg
     3421 gcttcggaag ccgcttcaaa acggtcgcaa cggatcaggc ttgccccgcc agccttttgt
     3481 cctttttcaa acatggcata aaacatttca ccgccttcgt gccaaatacg gaactcggca
     3541 cgcatacggt aatgtttgtc cggagattcg tacacttccc actcaggaac atccaaacct
     3601 gcaaaaaggg ttttaaggtg gtctttttta tcttgaagct gctgcgtgta gtcgctcata
     3661 tcgctatcct gaaaaatcag accggattat aagccgattc actgcaaaac acgcaacccc
     3721 tttaccggca tataacccgt tttcctccaa cttttatgct aaaatccgcc gtctccaaca
     3781 taccgccgac agaaggaaaa tatggcaggc aacactttcg gacaactctt caccgtgacc
     3841 acgttcggcg aaagccacgg cgcaggtttg ggctgcatca ttgacggctg cccgcccgga
     3901 ttagaattga gcgaatcaga catccaagcc gacctcgacc ggcgcaaacc cggcaccagc
     3961 cgccacgtta cccaacgccg cgaagccgac caagtcgaaa tcctctccgg cgtcttcgaa
     4021 ggcaaaacca ccggcacgcc catcgccctc ttaatccgca ataccgacca acgcagcaaa
     4081 gactacggca acatcgccca aagtttccgc cccggccatg ccgactatac ctattggcac
     4141 aaatacggca cgcgcgacta tcgcggcggc ggcaggagtt cggcccgcga aaccgccgcc
     4201 cgcgttgccg ccggagccgt cgccaaaaaa tggctgaaag aaaaattcgg cacggaaatt
     4261 accgcctacg tgacccaagt cggcgaaaaa gaaatccggt ttgaaggctg cgagcacatt
     4321 tcccaaaatc ctttttttgc cgccaaccat agccaaatcg ccgagctgga aaactatatg
     4381 gacagcgtgc gcaaatcctt ggattccgtc ggcgcgaaac tgcatatcga agccgccaat
     4441 gtccccgtcg gcttgggcga acccgttttc gaccgcctcg atgccgaaat cgcctacgcg
     4501 atgatgggca tcaacgccgt caaaggcgtg gaaatcggcg caggttttga cagcgtaacg
     4561 caacgcggca gcgaacatgg cgacgaactc accccgcaag gcttcctgtc caaccattca
     4621 ggcggcatcc tcggcggcat cagcaccgga caagacatcc acgtcaatat cgccatcaaa
     4681 cccaccagct ccatcgccac cccgcgccgc agtatcgaca tcaacggcaa ccccatcgaa
     4741 ctcgccaccc acggcagaca cgacccctgc gtcggactgc gcgccgcgcc gattgcagaa
     4801 gctatgctgg cattggtttt aatcgaccac gccctgcgcc atcgcgcgca aaatgcggat
     4861 gttcaggtta atacgcccga cattaccctt tcaaacaaat aaaaatttag ccaaaacaca
     4921 gactttataa tagaataccg agcattgccg tgccgctttg acgcacgggt ttgtttagag
     4981 gaatacaacc gaaatgacac aagaaaccgc tttgggcgcg gcactgaaat ccgccgtcca
     5041 aactatgagc aaaaagaaac agaccgaaat gatcgccgac cacatctacg gcaaatacga
     5101 tgtattcaaa cgcttcaaac cgttggcgct cggcatcgat caggatttga ttgccgcttt
     5161 gccgcaatac gattccgcac tgattgcacg cgtcctcgcc aaccactgcc gccgtccgcg
     5221 ctatctgaaa gccttggcgc gcggaggcaa acgtttcgat ttgaacaacc gtttcaaagg
     5281 cgaagttacc cccgaagaac aggcgatcgc gcaaaaccat ccttttgtgc agcaggcttt
     5341 gcaacagcag tccgcccaag ctgccgccga aacgctgtct gttgaagccg aagcagccga
     5401 atcttccgca gcagaataaa tccccaaacg aaatgccgtc tgaaaaccga tttggtttca
     5461 gacggcattt tttcgtatgc ggcaatcacg gttcaaatat ccaattccgc cgtatcgcct
     5521 tcgcgttcca tccaagcgcg gcgggcggcg gcttcgcctt tgcccatcag tttgacgaag
     5581 atgtcgcgcg tctcgtcatc tgcaccttct gggatttgta cctgcaacag gcggcgggtg
     5641 tcggggtgca tggtagtatc tttgagctgg tcggggttca tctcgcccaa gcctttgaaa
     5701 cggctgatgg aataggcggt ttctttaacg ccttcttttt gcagccgctc caaaatgctg
     5761 tcgagttcgt tttggtcgag ggcgtagaat ttgcgggcag gtttgctctt accttgtgcg
     5821 ttgacatcga cgcggaacag cggcggctgg gcgacgtaga tgtgtccgtc ggcaaccagt
     5881 ttcgggaagt ggcggtagaa cagggtcagc agcaaaactt gaatatgcga gccgtccacg
     5941 tcggcatcgg acaggatggc gattttgccg tagcgcaggc cgcttaaatc gggatggtcg
     6001 ttaataccgt gcggatcgac gccgatggcg acggaaatgt cgtggatttc ggcgttgccg
     6061 aagagttggt cggggtggac ttcaaagctg ttgagcactt tgccgcgcag gggcaggatg
     6121 gcttgggtgg ctttgtcgcg ggcgagtttg gctgagccgc cggcggaatc gccttcgacg
     6181 aggaagagtt cgttttcgcg gatgtcttcg ctttcgcagt cggtcagctt accgggcagg
     6241 acggcgacgc cgctgccttt tttcttttca atctttttaa ccgaacgcat ccgcgcctgt
     6301 gcttggcgga tggcgagttc ggcgattttt ttgccgaagt ccacgttttg gttcagccac
     6361 aattccaaag ggtcgcccga tacggtggcg acgagtttca gcgcgtcgcg gttggtcagc
     6421 ttgtctttgg tttgaccttg gaactgcggg tcgaggacgc gggcagagag aacgaaggcg
     6481 gtttttccga acacgtcgtc gctttgcact ttaacgccgc gcggcaagag gttgtgcaga
     6541 ttgatgaagt tgttgactgc gttgaacacg gcttgtttca agcctgcttc gtgcgtgccg
     6601 cccagcgggg tggggatgag gttgacgtag ctttcgttgg cgcacgagcc ttcttccagc
     6661 caagtcaggg caaacgccgc tccttcgccg atgctgaaat cgccgttgtg ttcgtctgaa
     6721 atgtagtttt cgcaagagaa cagcggtacg gcttcctgcg cgtcggcaat caggtcggtc
     6781 agatagcttt tcaggccgtc ggggtaatgc caggtttggg tgtgcgcttc gtcttcgcct
     6841 ttgaccggac gggtcaggga aacgcgcaca cccggcagca gcacggcttt ggcacgcagc
     6901 aggcgttcga gttcgggaat gctgtaattc gggctttcaa aatatttgcc gtccggccag
     6961 acgcgcactt ctgtaccgct gtctttgacg gcgcatttgc ccacttgtgc caacggttcg
     7021 accacgtcgc cgccggcaaa cacgatgcgg tggattttgc cttcgcgttt gaccgttact
     7081 tcaaggcggg tggaaagggc gttggtgacg gatacgccca cgccgtgcag gccgcctgaa
     7141 aaggcatacg cgctgccgcc gtcttttttg ttgaacttgc cgcctgcgtg cagacgggtg
     7201 aatacgagtt cgactacgga tacgccttct tcgggatgca ggccgacggg aatgccgcgc
     7261 ccattgtcgt gcacggaaag cgaaccgtct tcatgaattt gcacgtcgat tgcagtcgcg
     7321 aaaccgccca acgcttcatc cgacgcgttg tcgatgactt cttggcagat atgggtcggg
     7381 ctgtcggtgc gggtgtacat accgggacgt tctttgaccg gctccaagcc tttgaggacg
     7441 gtgatgctgg attcgctgta ttggttgttt ttagccatgg gaataatctg aaagtaagaa
     7501 aaacaacgct ttcagacggc ctgaaagcgt tgcgttccgt tgttttagcg gttgtcggaa
     7561 gattggcggg cggcaaagtc ttcataactt tccataccgc gcaggaagcg ggaagagagt
     7621 tctttcaacg cccccaaata aacgtcgcgt ttgaaatcaa tcacttcgtc aacgggtgcc
     7681 caatattgat gccaacgcca gccgtcaaat tcggggtggc gggtggcgcg caggttgaca
     7741 tcgcaatctc ggccggtcag gcgcaggaga taccaaatct gcttctgtcc gcgataagag
     7801 ccgcgccatt cgcggcgtac ccagttgttc ggcacgtcat aacgcagcca gtcgcgcgtg
     7861 cggccgataa ttttgacgtg ttgcggcaaa agcccgactt cttcgtacaa ctcgcggtac
     7921 atggcggttt cggggctttc gcccggcttg atgccgcctt gcggaaactg ccaagaatgt
     7981 tcgcgcacgc gcttacccca aaagacttcg ttgcggttgt tgattaagat gataccgaca
     8041 ttggggcgat agccttccct gtccaacacg gtgtcgccct ccgttaaatt caatcttggg
     8101 attttcccac aaatcaggcg gttttgacaa atcagacggc atggcggtac gcgtgccgaa
     8161 acacgggggg atttgggaaa atatcttaaa tttggtttac aataatgtat ttcaaattat
     8221 tcgggaatca gaccatgtta gatatccaat tgctccgcag caacaccgcc gccgttgccg
     8281 aacggcttgc acggcgcggt tatgactttg ataccgcacg ttttgacaca ctggaagaac
     8341 gacgcaagtc cgttcaggtg aaaaccgaag aattacaggc ctcgcgcaac agcatttcca
     8401 aacaaatcgg cgcactgaaa ggtcagggca aacacgaaga agcgcaggcg gccatgaatc
     8461 aggttgccca aatcaaaacc gatttggaac aggctgccgc cgatttggat gccgttcaaa
     8521 aagaattgga cgcatggttg ttgagcatac ctaacctgcc gcacgaaagc gtacctgccg
     8581 gtaaagacga aaccgaaaac gtcgaagtcc gcaaagtcgg caccccgcgc gaatttgact
     8641 ttgaaatcaa agaccatgtc gatttgggcg aacctttggg tttggatttt gaaggcggtg
     8701 caaaactctc cggcgcacga tttaccgtga tgcgcggaca aatcgcccgt ctgcaccgcg
     8761 ccttggcaca gttcatgctg gatacgcaca cgctgcaaca cggctacacc gagcattaca
     8821 cgccttatat cgttgacgat acgacgctgc aaggtacggg ccaactacca aaatttgcgg
     8881 aagatctgtt ccacgttacc cgtggcggcg acgaaaccaa aaccacccaa tacctgattc
     8941 cgacagccga agttaccctg accaataccg ttgccgacag cattatcccg tccgaacaac
     9001 tgccgctgaa gctgaccgcg cattcgccct gtttccgcag cgaggcgggt tcgtacggca
     9061 aagacacgcg cggtctgatt cgccagcacc agttcgacaa agtggaaatg gttcaaatcg
     9121 ttcatcccga aaaatcatac gaaacgctgg aagaaatggt cggccatgcc gaaaacatcc
     9181 tgaaggcttt ggaactgccc taccgcgtga ttaccctgtg taccggcgac atgggcttcg
     9241 gcgcggcaaa aacgtatgac ttggaagttt gggtgccggc gcaaaatacc taccgcgaaa
     9301 tctcaagctg ctccaactgc gaagatttcc aagcccgccg cctgaaggcg cgtttcaaag
     9361 acgaaaacgg caaaaaccgc ttggtacata ctttgaacgg ctccggcttg gctgtcggca
     9421 gaacgctggt cgccgtattg gaaaaccatc aaaacgccga cggcagcatc aacatccctg
     9481 ccgcactgca accgtatatg ggcggtgttg ccaagttgga agtcaaataa gtttgcaggc
     9541 tgcctgaacg tcaaatgccg tctgaaacct gtttcagacg gcatttcctt taaactttta
     9601 aaacacgtca gccgtcggca cgaaccgcat tgccgcaatc gccggtctgt ccgacctcgc
     9661 ggatattgga cagcgtaact tccgaaatat tacccaacgc ctcttccgtc aaaaatgcct
     9721 gatggccggt aaacagcaca ttatgacaag acgacaggcg gcggaacacg tcgtcggtaa
     9781 tcacatcgtt ggatttgtct tcaaaaaaca gctcgcgctc gttctcgtac acatccatgc
     9841 ccaatgcgcc gattttccgg cgtttcaacg cctcaatcgc ggcggcactg tcaatcagcc
     9901 cgccccggct ggtgttgata atcatcacgc cgtctttcat tttgtcgaac gccgcttcgt
     9961 tcagcatata gtggttttcc ggcgtggcgg ggcaatgcag cgtgatgatg tccgaccggg
    10021 catacagctc gtctaaatcc acatatttgc cgccgatttt ttccgcttcg gggttgcaaa
    10081 acggatcgta agccagcagg ttcatgccga aaccctttaa aatccgcatg gttgcaatac
    10141 cgattttccc cgtgccgata acgcccgccg ttttgccgta catattgaaa ccggtcagac
    10201 cttccagcga aaaattcgca tcgcgggtac gctgataggc tttgtggata cggcggttca
    10261 acgtcagcat cagaccgacc gtatgttccg caaccgattc gggcgaatag gcaggcacgc
    10321 gcacgacttt caagcccaac tcttcagccg cctttaaatc cacattattg aagccggcac
    10381 aacgcaacgc cacagttttc acgccaattt gcgccaattt ttccaacacg ggccggctgc
    10441 cgtcgtcgtt tacaaaaata cagaccgctt ccgcgccttc cgccattttc gccgttttcg
    10501 catccagcat aaaatcaaaa aactccagct cgaagccgaa atgccggttg gcgcgggtaa
    10561 aatgttcgcg gtcatagctt ttcgtaccgt aaatcgcaat cttcatcaat atgtccagtt
    10621 gtcgtctatg gttgagaaac ggcataatac actgaattca aacaaatcag tagaatatgg
    10681 tggattaaaa ttgattgcat gcacggcatt tccatttcaa aacacaaaac tcaatcgccc
    10741 attgccgcca gaagctcggc ctgatgctcg gcaatcaggg cattggtgat ttcttccaag
    10801 tcgccgtcca tcacaaaatc cagcttgtgc agggtaaggt tgatgcggtg gtcggttacg
    10861 cggccttggg gatagttgta ggtgcggatg cgttcgctgc ggtcgccgct gccgatgagg
    10921 gatttgcgtt cggcggcttc tttggcttgg gcttcgcgtt tttgcgcgtc gttcaggcgg
    10981 gcggcgagaa ctttcattgc ctgcgctttg ttggcgtgtt ggctgcggcc gtcttggcat
    11041 tcgaccacca tgccggtggg caggtgggta atgcggacgg cggaatcggt tttgttgatg
    11101 tgctgaccgc ctgcgccgga tgcgcggaag gtgtcgatgc gaaggtcgac ggggttcaac
    11161 tcgatgtctt ccagttcgtc cgcttcgggc atgacggcaa cggtgcaggc ggaggtgtgg
    11221 atgcggcctt ggctttcggt ggcggggacg cgctgcacgc ggtgtccgcc cgattcaaat
    11281 ttcaaacggc tgtacgcccc gagtccgaca atacgggcga tgacttcttt atagccgccc
    11341 aattcgcttt cgttggcgga cacgatttca acctgccaac ggttgcgctc ggcgtagcgg
    11401 ctgtacatac gcagcaaatc gccggcaaac agcgcggctt cgtcgccgcc cgttccggcg
    11461 cgtatttcga tgaagatgtt tttgtcgtcg tcggcatctt tgggcagcag cagtttttgc
    11521 agttcggtat cgagttcgcc gattttggct ttggccgctt cgatttcttc ggcggcaaag
    11581 tctttcattt cggggtcgga caacatttct tcggcatccg ccaagtcgct ttgggcaagc
    11641 cgatagtttt ggaacacttc gacgacgggg gtcagttcgg cgtgttcgcg cgtgagcttg
    11701 cggtagttgt ccatgtcgga cgtggcttcg ggctgtccga gaaggtgggt aacttcttcc
    11761 agtcggtcgc tgagttgttg tagtttttct aagatagacg gcttcataat tcttccataa
    11821 caaacgccgc ctgaatgttc agacggcatc aacactggat tattataata ggttttccgg
    11881 atattcaaaa agataatctt agatggataa cctaccgtcc caacagggca tcggcattgc
    11941 gctccgttac ctttgcaatc tcttctacac aggttccgcg gatttccgca gcaatctttg
    12001 caatacccgg aatattggca ggcgtattaa tctctttttt cagcataaac gggctatccg
    12061 tttccaatac gaaatccccg tcgttcaagg ctttaagcgt atcgcgcact ttacgcgcgt
    12121 tcggattgag cagcagcgaa ccgatgccga ttttgaaacc cagtttcgtc aacacacgcg
    12181 cttcttccgc gctgccggag aaggcgtgaa cgatgccgcc ttgggcaaag cctgtctgtt
    12241 tgacggcggc ggcgatgtcg gcggtggctt tgagattatg gataatcacg cggcggcgca
    12301 gggtttgcgc aatttcaagc tggcggacga aaacttgaat ttgccgttcg cgctgctgcg
    12361 acgtttgggt tttatcgtaa aaatccaagc cgatttcgcc gacccatgcc tgcggataat
    12421 gtgccaacat cgtttccagg cggacgaaat cccgctcggc aatgccgtct gaaaaccaag
    12481 gatgaatgcc cagtgcaata cggatttgac cgtgttcgga cggcatttcc gccaaatccg
    12541 ccacgtcctg ccaatcctgc gggcgcgtcg cgggaacgat aaaccgcttc accccaactt
    12601 tccgcgctgc ggtcaggatg tgcggcaggt tttcgcgcag ggcgggatca gcgagatggc
    12661 agtgggtgtc ggtgaagttc atttcgattt cacactaact ttagtcttac caattctttg
    12721 taaacatctt ccttacccca gccttgcgat acggcgaggg tcatcagcgc ggtggcggtt
    12781 tcgaggttgc atttgccgcc gttgatgatg cccgccgagt tgcggaacgc gttgccttgc
    12841 gcgtaaacgg cggcggtttt gccttgtcgg acttggctga tgttgagcag cagtttgccc
    12901 tgccgcgcga agtctcggac ggcgcggata aaaccttcgt ctgcgggcgt gttgccgtgt
    12961 ccgtagcttt gcaggataag agcttgggcg ggaagctgtc cgagtccgtc cgcaagttct
    13021 tggacggcaa agccggggat aagcgtgcgg acagcgattt ttgcctgcgg gtcgggataa
    13081 cggattttga ggccgtctga aacggctgct gcgtcttggg acgggaggcg aagattgtgc
    13141 caaccccggg tttcgtccca ttcggcaagc gtgccgaaat gcggattgtc gaagcctgca
    13201 gcagtttcgg tgctgacttt gctgctgccg acggcgggat acagtttgcc gtcaaacgcg
    13261 atgacggttt gtttgagctt gaggctgaag gcggcaacgg cggtggagag gttgcgcggg
    13321 gcatcgctgt tttcggcggc gtaaggccat tgggaaccag tcaggacaat cggtttgccc
    13381 aaaccttgca gagcgagcgc gaggagattg gcggtgtacg ccatgctgtc cgtgccgtgc
    13441 agtatcagga tgccgtcgca tgaagggagt ttgtcggcaa tgatgtccag ccaatcgcgc
    13501 cagtgttgca gcgtaacgga ggaggaatca atcaagggat tgcagacgtg ccactcgaaa
    13561 tcgaggccgt ctgaaaaggg ggaaagggct tggctaacca gtgcggtatc ggggcgcagg
    13621 ccttcgctgc tttgggtcat gcctatggtg ccgcctgtgt agaggacgaa gattttttgt
    13681 ttcatggaca tcatcgggtc gtctgaaaat aataatacgg cttatttaac tatatttcgg
    13741 acagactggc aatttggcgg cgcggacggt tttcagacgg ccttcaaatg aaaaagcacc
    13801 cgagggctgt cgatatttga ttttccaagt agatttttat tcacgaaata ggagagccgc
    13861 aacaagctta aatcccttgt gaggttccca acacggaaga taccgctttg tggattaaaa
    13921 aatacggaaa ctattgaata tcgacaacct atttaggtgc ttgattttat tgtttgcttt
    13981 gcgcggcttt tttggctgcc ttggcggctt tgcgttgcgc ccgcttttct ttcaatttgc
    14041 tgcggtaaaa ctggatacgt tggcgttttt tccaccaaat ccaagcgaca acggtcgcac
    14101 ctatacccaa gataacaaaa atacccgatt gcaggctgtg cattttcgcc atcagccaat
    14161 cgatgttgtg cgcgccgtac tcgcccaagt aaatccaaac gggcacggaa atcagcgcgg
    14221 caagcccgtc cataatcaga aagcgcagat acgatacttt gcggctgatg ccggcggtaa
    14281 cgaaaacggc agtccgcaaa cccggcagga agcgggcgac aaacagaacc cagttgccgt
    14341 atttgtcgaa tttttcctga acctgtgcgt aacgtttcgg cgtcatgatg cgcgcaatcg
    14401 gtttgaactt gaggattttc tgcccccaaa ttcgtccggc ggcgaacatg atgccgtcgc
    14461 ccgccaacac gccgagcata ccgactgcaa acataatatg cggattggta taacccatac
    14521 ccgaaatcac gccgcctgtt accaaggtca aatcctcggg aatcggcacg ccgaaaccgc
    14581 agatgaccaa aacgaaaaac acggccgcat agccgtattc gacaaaaaag gcttccaaaa
    14641 gggcaaacat agcggatatt ccattgtcgg agataaaaag tcagaacaag ccgaaacatt
    14701 ttctgcgcga aacggcattc tatcaaagat tatgccgtct gaaagcggca aggggcagat
    14761 tcgcgccctc tgccggacgt tcagacggca tttaaacagg aaaaccgccg ctgttgaaac
    14821 ggttttgcct gattttgcct aaaggctgcc gatggcggaa gcaatacgtt ccgcaccgtc
    14881 gcgcgcccaa tccgcctgcc gcgcctccac catcacacgc acgacgggtt cggttcccga
    14941 agcgcgcaac acgacacgcc ctttgccttc gagttctttt tccacttccg ccaacacgtc
    15001 ttttgaggct tcctgccagt tttgcccttt gccgatacgg acattaatca tcgtttgcgg
    15061 atacggctgc caatcggcgc aaacggtggc gaggtcttgg ttcagcgttt gcagtgccgc
    15121 caaaacttgc agcgcggaaa taatgccgtc gccggtgttg tgtttgtcca tgcacaaaat
    15181 atggccgctg gcttcgccgc cgatgagcca gctgcgttgg ttcagctgtt ccaacacata
    15241 gcggtcgccg actttggcgc ggcagaaatc cacgccctgc tctttcaggg cgatttccat
    15301 cgccatattg gtcatgaccg tgccgaccac gccgccgatg ttgatacctt ctcgggcgcg
    15361 ggctttagca atgacgtaaa tcaggctgtc gccgtcgtaa accttaccgt ttttatcgac
    15421 catcatcagg cggtcgccgt cgccgtctaa ggcgatgccg tagtcggctt catgctgtaa
    15481 aacggcagcc tggagtgtct tggtataagt cgcaccgcat ttttcgttga tgttgtagcc
    15541 gtcgggttcg tcgccgatgc tgacgacctg tgcacccagt tcgtgaaaca ccttgggtgc
    15601 gacaccgtac cccgcgccgt tggcggtatc gacaaccaac ttcaaacccc gaaggtcgga
    15661 atggctggga aaggtggatt tgcaaaattc gatatagcgg tcgtccgcac cgctgatgcg
    15721 gcgtgcgcga ccgagacggg cggacggttg ggttttcatt tcttcgtcga ttttggcttc
    15781 gatttccaac tcgatttcat cggaaagctt cacgccgcct tcggcgaaga atttgatgcc
    15841 gttgtcggaa taggcgttgt gcgacgcgga aatcatcacg ccggcggaca ggcgcaacgc
    15901 gcgggtcaga taagccacgc cgggcgtggg cagcggtccg gtctgtacca cattcacacc
    15961 cgccgccgta aaaccggcca ccaaagcggc ttccagcata tagccggaaa tgcgcgtgtc
    16021 tttgccgatg aggacggtcg gtttctggtc ggtgtcgtgc tgcaccaaaa cctgccccgc
    16081 cgcatagccg agtttcaata cgaaatcggg cgtaatcgga aattgcccca cttcgccgcg
    16141 cacgccgtcc gtgccgaaat atttttttgc catgtgttgc tccgagaatg tgaaccgttg
    16201 tccgagatta tacagtcagt ttgtgccctg ctgtctgcac cgttgatgcc gtctgaagcc
    16261 gccccgtcct tttcagacgg catgaagtat gcgaaccgct gtttacagat tgatgcccaa
    16321 cgcttcccac accttcaacg cgtccgccgt cgccttcaca tcatgcaccc gcacgatttg
    16381 cgcgccgcgc gccacggaag ccagcgccgc cgccacgctg ccgtgtacgc gttccgccgc
    16441 gtctgcctca ccggtcagct cgcctatcat acgtttgcgc gacacgccga tgagcagcgg
    16501 aaaacccgtt tccgccatca attcgggtaa atgccgcatc agtgcgatat tgtgttgcaa
    16561 ggttttgccg aagccgaaac cggggtcgag cgtaatccgt tgcggcgcga tgcctgccgc
    16621 gacacaggtt tctgaccgtg ttttcagata acgtgccact tcaccgacaa catcctgata
    16681 tttcggatta atctgcatgt tttcaggcaa accctgcatg tgcatcaggc aaatgcccgt
    16741 gtccgcctga cgcgccagca attcgaccgc gccttcgtcg gtcaacgccg ccacatcgtt
    16801 aataatatcg atgccgccga gtgccaacgc tttttccata accaccgtgc ggcgcgtgtc
    16861 caaactgacg ggaacgcccc accccgccac ttctgccaat acaggctcaa cccgcgccca
    16921 ttcttcttca ggagaaacat aatccgcacc cggccgcgtc gattcgccgc cgatgtcgag
    16981 aatgtccgca ccctctttca aaagccgttc ggcgtgcgcc aaagcggttc gggcattttg
    17041 cgaatacgcg ccgccgtcgg aaaaagaatc gggcgtgaga ttcacgatgc ccatgatttt
    17101 cggcttgtcc aaaccgattt caaaccgtcc tgcctgccaa acgtgtcgtg ccatctgaac
    17161 tcctcccaaa ataaaaaaca gattatatgc cgtctgaaac cgtcttgtgc gcttcagacg
    17221 gcaccgctat tcgggcggca gacggcatgt tgtccgaatg tctgctccgc ctttgaatct
    17281 gccggtatgc ctgctatccg cccgactttt caaaacaggt tccgacgatt ccgcacgcgc
    17341 ctgccgcctt tgccaagccg tacaggattt cctgcggcat atcgcggttc cataatcccg
    17401 taatattcgc aatcacgggc agatggctga tttggcggac tttcacgatg gattcgacat
    17461 ccaaacggta gggatggtct ttggtatggc tcaacacgcc cgactcgccc agaatcaggt
    17521 ggcggttgcc gcgcgagaca acatattctg cggcattcaa ccaatcttcg gcagaatggt
    17581 gcttgtcttt acacagcacc aagggaatgt tcaggcttcc ggcttcattc aatacggcaa
    17641 gatcggacat cagcccgccg cccaaataca ggatgtccgc ccccgcattc aaagccgctt
    17701 cgacatggcg gacgttgcgg acgcgcacca atacgggttt ccctgcgcca tgcgccgatg
    17761 cggtctgttc cgccaaccgt ctgcaccgtc cccgcccttc atccgcactt gaagtgtcgt
    17821 ataagtttgc cgaagtgaaa aacggatcca gaaacactgc atccgcattg cgccatactg
    17881 acggttctgc ggcgatacgg acggtttccc cgccgccgaa agccacgcct ttggcggcaa
    17941 cgcggctgtc ttccgcccga ttttcccgac tgacggtttt ccatgtatcc aaaatgcgga
    18001 cggctttctc gacctccggc agcgtctgca cctccctgac gctcaaaacc ctatcgtcgc
    18061 cgattgcgcc gatgacggta cgctcgtcgc cgtgagaaat gtgttctcgc agacctctgc
    18121 tgcggataaa ggcgacaacg ccggcaatgt ccgcttcggc ggcacgcctg ctcatgacaa
    18181 taatcatatt tcctcctgac acaagaaacg gcctgcccaa aataggattt ttgcaagccg
    18241 tgttatactg tggcgtgttt tacagattgt tcgggctatg gatttattat cggttttcca
    18301 caaataccgt ctgaaatatg cggtggccgt gctgacgata ctgcttttgg cggcagtcgg
    18361 gctgcacgct tccgtatatc gcaccttcac gcctgaaaac atccgcagcc gcctccaaca
    18421 aagcattgca cacacacacc ggaaaatctc gtttgatgcg gacattcagc gcaggctcct
    18481 gccccggccg accgtcatcc tgaaaaacct gaccattacc gaacccggcg gcgaccagac
    18541 tgccgtttcc gtccaagaaa ccaaaatcgg attgagctgg aaaaacctgt ggtcggatca
    18601 gatacagatt gaaaaatggg tggtttcgag tgcggaactt gccctgacgc gcgacgggaa
    18661 aggtgtttgg aacatccaag acctaatcga caacccaaaa cgcaaagcct cggtaaaccg
    18721 catcattatc gaaaacagca ccgtccgcct cgacttcctg caggaacagc ttatcctgaa
    18781 ggaaatcaac ctcaacctgc aatcccccga ttcgtcgggg cagccgtttg aaagttcggg
    18841 catactggtt tggggaaagc tgtccgtccc gtggaaaagc agggggctgt tcctttcaaa
    18901 cggcatcggc ccgcccgaaa tctcaccgtt ccattttgaa gcttccactt cgctggacgg
    18961 acacggcatt accatttcca ccaccggcag cccttctgtc cgcttcaacg ccggcggagc
    19021 ggatgccgct ggcctcggcc tgcgtgcaga cacttccttc cgtaacctcc acctgaccgc
    19081 ccaaatccct acgctggcac tcaggaacaa cagcattaaa attgaaaccg tcaacggcgc
    19141 atttaccgcc ggcggcgaat atgcccaatg ggacggttcg ttcaaactcg acaaagccaa
    19201 cctgcactcc ggcatcgcca acatcggcaa cgccgaaatc tccggcagct tcaaaacacc
    19261 gcgccaccag accaacttct ccctcaattc gccgctcgta tggacggaaa acaaagggct
    19321 ggacgcgccg cgcctgtatg tatcgaccct tcaggatacc gtcaaccgcc tgccgcaacc
    19381 ccgtttcatc agccggctcg acggttcgct gtccgtaccg aatctgcaaa attggaatgc
    19441 cgaattaaac ggcacattcg accgccaaac cgttgccgcg aaattcagat acacacatga
    19501 agacgcaccg catctggaag ccgccgtcgc actgcaaaaa ttgaacctga ccccctatct
    19561 tgacgacgtg cggcaacaaa acggcaaaat atttcccgac accctcgcca agctgtccgg
    19621 cgacatcgag gcgcacctga aaatcggaaa agtccaactt cccggcctgc aactggacga
    19681 tatggaaacc tacctccacg ccgacaaaga ccatatcgcg ctcagccgtt tcaagtcagg
    19741 gctttacggc ggccataccg aaggcggcat cagcatcgcc aacacccgtc ccgccactta
    19801 ccgcctgcaa cagaatgcaa gcaacatcca aatccaaccg ctgctgcaag acctgttcgg
    19861 cttccacagc ttcagcggca acggcgacgc ggtcatcgac ctgaccgcgg gcggcgaaac
    19921 ccgaaaagag cttatccgct cgcttcaggg cagcctgtcg ctaaatattt ccaacggcgc
    19981 gtggcacggt atcgacatgg acaatatcct gaaaaacggc atttcgggca aaactgccga
    20041 caatgccgca cccagcacac ccttccaccg attcacgctc aacagcgaaa tttcagacgg
    20101 catcagccgc cacatcgata ccgaactctt ctccgacagc ctctatgtta ccagcaacgg
    20161 ctataccaat ctggatacgc aggaattgtc tgaagatgtc cttatccgca acgccgtcca
    20221 tccgaaaaac aaaccgattc ccctgaaaat caccggtacg gtggacaagc cgtccattac
    20281 cgtcgattac ggcaggctga ccggcggcat caattcgcgc aaagagaaac agaaaatcct
    20341 cgaagacacc ctgctggaac aatggcagtg gctcaaacct aaagaaccgt aaacatcctg
    20401 cgtacaaaaa tgccgtctga aacgcccccg cgcttcagac ggcagaccgt aaaacctaca
    20461 accccaattc cccccaaatc ccatcaatct tagccgtaac cgcagggtct tttttgatga
    20521 cgcgtcccca ttcgcggtcg gtttctcccg gccatttgtt ggtcgcatcc aaacccattt
    20581 tgccgccgag tccgctgacg gggctggcga agtcgagata atcgatgggc gtgttttcca
    20641 ccaacacggt atcgcgtact gggtccatgc gcgtggttac cgcccagatg acttccttcc
    20701 agtcgcgcac gtttacatcg tcatcgacca cgatgatgaa tttggtgtac ataaattggc
    20761 gcaggaacga ccagcagccc atcatcacgc gcttggcgtg tccggcgtac tgttttttca
    20821 tgctcaccac cgccatgcgg taggagcagc cttcgggcgg caggtagaaa tcggtgattt
    20881 cggggaactg cttttgtaaa agcggtacga acacttcgtt caacgccacg cccaaaacag
    20941 cgggttcatc gggcggtttg cctgtgtagg tggaatggta aatcgggttt tcgcgcatag
    21001 tgatgcgttc gaccgtgaac acgggaaaat gatcctgctc gttgtaatag ccggtgtggt
    21061 cgccgtacgg gccttccaac gcggtttcgt tcggatggat gacgccttcc aacacgattt
    21121 ctgcgcgggc aggcacttgc aaatcgttgc cgatacattt caccagctcc gtccgcgaac
    21181 cgcgcagcag tccggcaaac tggtattcgc tcaaagtatc gggaacaggc gttaccgcgc
    21241 ccaaaatggt ggcggggtcg caaccgagca cgacagcgac gggatacggc gtatcgggat
    21301 tgagtttgcg gaattcctga taatccaacg cgccgccgcg atgcgacagc cagcgcataa
    21361 tcagcttgtt tttgccgatt agttgttggc ggtaaatgcc gagattttgg cgttttttgt
    21421 gcggcccgcg cgtgacggtc aagccccatg ttaccagcgg cgcaacgtct tccggccagc
    21481 aatgctgaat cggaagttta tacaaatcaa cgttttcgcc ttcccacacg atttcctgac
    21541 acggcgcgtt tttcaccacg ttcggcgcca tgctccaaat gtctttcagt agcggcagtt
    21601 tggaaaacgc atctttgatg cctttgggcg gttcgggttc tttcaaatac gccagcgtct
    21661 gcccgatttc acgcagcttg gacacgctgt ccgcgcccat gcccatcgcc acacgttcgg
    21721 gcgtgccgaa caggtttgcc aacacaggat aaccgtagcg cgtaccgtcg ggcttaaccg
    21781 ggttttcaaa cagcaacgcc ggcccttcgg cgcgcagcac gcggtcggcg atttcggtca
    21841 tttccaaatg cggggaaacg gggtgcgcga tgcgtttgag tttgccctgc tgctcgagca
    21901 tggcgatgaa gtcgcgcagg tctttgtatt tcatattcat cctttttgtc cttttatcct
    21961 gagcaatccg attcggatac cgcccctatc cttgcctgcg cttcggcata ttctatgccg
    22021 tgataaaagt cgcgtaccag cggatgttcg ctgccttgat ggagttgcaa caaaggacgt
    22081 tgaccatcgg gttgggtaac gacattgcaa tgcaaaccga aggtgtcggt ttcataaggg
    22141 ggcagccggt tgcagatcat gccgaaataa acagcgtttt ccggattgtc gtgaaagtgg
    22201 ctttgatagt cgttaaaact cttttcgctg acggacaccc acacgccata ttccagcgtt
    22261 tcctcatgcc cgataatcgg aatcggcagc accgcgcgga taaagcggtc ggtttggtcg
    22321 ggatagcgga tgatgcagaa atcagaatcg cattctgctt gataagcaat gcgttcttct
    22381 tcactgagtt gattataggg atcgggggcg gtaaagccga ttgcgggcat atcttcatgt
    22441 ttttcgccgc aggaagtgca agtgtacata aggtttcaga ctttcaaaac gagtttgcgg
    22501 taaagccatt cgccggcaaa cagcatcccc atcaatacat aggcaatcac gcctgtataa
    22561 accgcccacc aatcatatcg ccccaaccgg gccaacaaag cggcaagcgt cccgttgatt
    22621 ataaaaaata cgcaccaaac ctgcgttacc cggcgggtat agcgcacggc tttttcaggc
    22681 aggtcgggct gttgcagccg cgcaagtttt tcaatcaccg tctgcccggc aaacaggctg
    22741 ccgccgaaca ccgccaacat catcagattg acgaggacgg gataccaata catcgaatca
    22801 tgccgcccga acaccaatac tgcggcaaaa aacagtgtaa taaacaaagc cgcataacgc
    22861 tgttggggca gtttggcagt cagggcgcgc agcagccaca acccgcacat cgccgccgcc
    22921 agccaaacaa accagcccgc ctccctgccg tatatagtgg attaacaaaa accagtacag
    22981 cgttgcctcg ccttgccgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat
    23041 ccactatacc acaaagcggg ataggcaatg cttaatacgg tcagaaaaat atgtccgaaa
    23101 aaaccgggtt tcattttgaa tccgcacaaa tgttttcaga cggcatccga taaaaacatg
    23161 ccgtctgaaa aataattaga aatacccgat tagcccgcct gaatcttcaa taccgcctgt
    23221 accacgtcgt tgacggtgcg gacattgcgg aaatcttcgg cctgcagctt gcggccggtt
    23281 tcgcgcttga tgcggtcaat caggtcgatg gcatcgatgc tgtcgatttc caaatcttcg
    23341 taaagattgg tatcaggcgt aatccgttcc ggttcgattt caaacaactc ggtcagggta
    23401 tcgcgcaaca accggtagat ttcttgttcg gtcatatgtt tcatccttat gcttggcggc
    23461 ttttgacaaa ggcggcgagt gttttgacat tggcaaaatg ttcgcgcaag ttctcctgct
    23521 cgccgtccaa tcggaaaccg aaatgttttt gcaccgccaa gcccaattcc agcgcatcga
    23581 cggaatccag tcccagccct ccgtcgccga acagcgcgtc ttcgctgccg atgtcggcgg
    23641 cggttatatc ctccaaagcc aaactgtcga taatcagttg tttgatttgg ttttccaagt
    23701 cgttcatatg gttttccttg taaaatagtc ctgcagatat tcattcagcc gccgcgccgc
    23761 aatcggcaac ggtttctcgg cgagccagtc ttgggggagg atgtcgtctc cgacggtaat
    23821 ttcataccgt atccttttcg gggggatgcg gtaccacggc tgcccctttt taaaattggg
    23881 cggattcatt ttgatacata cgggcgtaat cacttcggca taccgcagtc ccaaagaaac
    23941 cgcgccccgg tgcattttta cccgtccgtc ccaccccgtc ctcgttcctt cggggaacac
    24001 cagcaggctc tgcccgctgt caaaaaccgc ctttaccgtt tccagcattg cttccgactc
    24061 ttcgttcgga atatagcccg cacctttaat ctggctgctc attgccggat tgtgctgcaa
    24121 atcttttttc acgatacagt tcattttggg cacatggccg acaagcagca ccacatccag
    24181 caaagacgga tggtttgcca aaatcaactg tcccgggcgg ttgagttttt caacaccctt
    24241 gaacgatacc tccaacacgc ccgaccattt cagataagca acgaacaaac gccaagatgt
    24301 tccgataatc cggcgcgccg ccaattggcg ggcgacagac cctgaagtgc cgttcaaagt
    24361 ataaggcaac aaaaccaatt tcatcataat gccgccgaca ccgaaaatca caaatcctaa
    24421 ccaagtcgca aaaaaacggc ggcaataatc caatttatcc attccgctgc cacagccatt
    24481 cacgattgcg atatacccgg cggcattctc gactgccgtt cagcagaaag cgcacccatt
    24541 ccaaaccgct ccaatatgcc tcgggcagca taccggcttc agacggcata tcgtccgaag
    24601 cagacaaagt caggctgtaa cgcgtccctt tggtcagaac catcgccaaa gcataagcaa
    24661 acggcgcgcg agtcgccgat acggcatatc cttccggcag cggatcgtcc gccgccaaaa
    24721 ccaaaaccga cccgcatccc tcttccaaca gtgatgccgc ttccgccaat gccgtttcca
    24781 caccgtcggc acacacggcc aatgccgtct gctcgctcat atcctgacgc aatatcgacc
    24841 attgccccgc cgttgcattg tgcaccgaca aaccgaacga agtcggcgac acggtatgcg
    24901 atttcaacag ttccaaccac aaatcgaaac tgcgtgccat ttccccgtcg tgcgaggcat
    24961 aaactaccgg actgccggga tgggcggagg caatgtccca agccgcgtcg cataccaaac
    25021 gcgccgcctt actcaaacgg cggcgctgca tagcgggcag gaacggcaat tccggcctga
    25081 catcgggcaa accgtcggca aaatccggac attccgccca ttttgcccac tgggccatat
    25141 cgcgcatttt gctgcccgaa acccgccagg cggcgatgtc gaagtggaac cggcaaataa
    25201 tcgacggcat agtttctttc aaaaatttac actgtgccgc attctaacca aagcctatcc
    25261 ccctgacaat gccgaaattc aaacgcattt ctgccccctt tctccgacaa cgccgcccct
    25321 cggaaaaccg ccagaattag cctgaattta catttatcat tataatgccc gtatttgcca
    25381 gcctgccgcc gcaatatatg gacacactgc tagaatgccc gattaccaac accgcctccc
    25441 tgctgccgca cagcgggcgt atggttctga tagaccgcat tacccgatac ggcgatgatt
    25501 ttgtcgaagc aggggcacag gtaagcccca atcacatcct tttacttgac gacaaactgc
    25561 cctacacggc atttatcgaa ctgatggcac aggctgtcgg cgcgtatgcc ggtatccaag
    25621 cccgaaaaaa cgcacggtcg gtccggctcg gcttcctgct cggcacgcgc aaacttgaaa
    25681 tcttcgccca atccgtccca atcggcacgc atctgctggc aacggcgcat atgtctattc
    25741 aggatgccgg gggtatgggc gtgtttgact gcgaactgcg ttggacagac gcgccggaaa
    25801 cttcgtccga aacactccct tcagacggca ttttggcgcg cgcctcactc aacgtgtaca
    25861 gccccgaaca ccctgccgga acaaccgatg ccgtctgaac aggcacacac atacggagaa
    25921 caacgatgac cgaaactgtc ctgattaccg gctccaacag gggcataggc aaagccgtcg
    25981 cattcggttt ggcggaagac ggctttgata tcgctgtcca ctgccgcagt cgccgcgacg
    26041 aagccgaagc cgtggcggaa gaaatccgcg ctttgggcag aaatgcgcgc gtgttgcagt
    26101 ttgacgtgtc cgaccgcgaa gcctgccgcg agattctgac cgccgacatc gaagcaaacg
    26161 gcgcgtatta cggcgtggtg ttgaacgccg gactgacgcg cgacaatacc ttccccgcgt
    26221 tttcagatga cgattgggat gtggtgctgc ggactaattt ggacggtttt tacaatgtat
    26281 tgcatccgct ggttatgccg atgatacgcc gccgcaaagc cggacggatt gtgtgtatgg
    26341 catcagtgtc cggcctgacg ggcaaccgcg ggcaggtcaa ttacagcgcg tcaaaagcag
    26401 gcattatcgg cgcggcaaaa gccttggcgg tcgaactggc gaaacgcaaa atcaccgtca
    26461 actgtgtcgc gccgggtctc atcgataccg atattatcga tgagaacgta cctgtcgaag
    26521 aaatcttaaa ggctgtcccc gcagcgctta tggggctgcc ggaagaagtg gcgcacgcgg
    26581 tgcgtttcct gatggatgaa aaagcggcgt acatcacgcg ccaggtgatt gcggtgaacg
    26641 gaggtttgtg ttgaatacca gaagggtcgc agtaacaggc ataggcggca ttaccgcctt
    26701 cggccgggat tggcaaagca tacaggcagc attcaaagcc gaaaaaaacg ccgtcaaata
    26761 tatggattgg cacgaacgtt tccccgaatt ggaagcgcaa ctgggtgcgc cgattgaaaa
    26821 ttacgcgccg ccgaaacatt ggacgcgcaa gcagctcaga agtatggggc gcgtgtcgta
    26881 cctgtgcgtc gatgcggcgg agcaggcgct ggcggatgcc ggtttgctcg gggacgaaag
    26941 cattaccgac ggacggatgg gcgttgcctg cggctcttcc agcggcagca ccaaagacat
    27001 cggcgatgtg ggcgaattgt tgctgaccgg cacgtcgcgc aacttcagcg ccaacaccta
    27061 tgtgcgtatg atgccgcaca ccaccgccgc caatatcggc atctttttcg ggctgaaagg
    27121 gcgcatcatc ccgacatcga gcgcgtgttc gtccggcagc caaggcatag gttatgccta
    27181 cgaagccatc aaatacggtc tgaccgatat gatgctggcg ggcggaggcg aagaattttt
    27241 cccgtccgaa gtgtatgttt tcgactcgct ttatgccgcc agccgccgca acggcgaacc
    27301 ggaaaaaacc ccgcgcccat acgacgcgaa ccgcgacggg ctggtcatcg gcgaaggcgc
    27361 ggggattttc gtgctggaag aattggaaca cgccaaacgg cgcggtgcga taatttacgc
    27421 cgaactcgtc ggctacggag ccaacagcga tgcctaccat atttccacgc cccgccccga
    27481 cgcgcaaggc gcaatccttg cctttcagac ggcattgcaa cacgcaaacc ttgcacccga
    27541 agacatcggc tggattaatc tgcacggcac cgggacgcac cacaacgaca atatggaaag
    27601 ccgcgccgtt gcagcggttt tcggcaacaa tacgccctgc acgtccacca agccgcaaac
    27661 cggacacacg ctgggcgcgg cggacgcaat cgaagccgcg ttcgcgtggg gcattgccga
    27721 ccggcaaagc aatcccgaag gaaaacttcc gccccggctt tgggacgggc agaacgaccc
    27781 caacctgccc gccatcaacc tgaccggcag cggcagccgc tgggaaaccg aaaaacgcat
    27841 taccgccagc tcgtcgtttg ccttcggagg aagcaactgc gtcttaatca tcggatgaaa
    27901 taagtttgtc aatcccaccg ctatgctata caatacgcgc ctactcttga cgggtctgta
    27961 gctcaggggt tagagcaggg gactcataat cccttggtcg tgggttcgaa ccccaccgga
    28021 cccaccaatt cccaagcccg gacgtatgtt tgggcttttt tgccgccctg tgaaaccaaa
    28081 atgctttgag aaaccttgat aatgaaaaaa gtcagcgtat tgattgttgc caaaaacgaa
    28141 gcaaaccaca ttcaggaatg tattgaaagt tgccgtttcg ataaagaagt catcgttatc
    28201 gacgactaca gcaccgacaa tactgccgaa attgccgagg gtttgggcgc aaaagtcttc
    28261 agacggcatt tgaatgggga tttcggagcg caaaaaacat ttgccatcga acaggcaggc
    28321 ggagaatggg ttttcctgat tgatgcagac gaacgctgca cgccggaact atctgatgaa
    28381 atctcaaaaa ttgtccaaac cggcgattat gccgcctatt ttgtcgaacg ccgcaacctt
    28441 ttccccaacc atcccgccac acacggcgcg atgcgtcccg acagcgtatg ccgtctgatg
    28501 ccgaaaaaag acagttcggt gcaaggcaaa gtacacgaaa ccgtacaacc cccctacccc
    28561 aaacgccgtc tgaagcattt tatgtaccat tacacgtacg acaactggga acaatatttc
    28621 aacaagttca acaaatatac ttccatttca gccgaaaaat accgagagca gggaaagccc
    28681 gtgcgtttcg ttagggacat tatcctccgc ccgatttggg ggtttttcaa aatttatatc
    28741 ctgaacaaag ggtttcttga tggaaaaatg ggttggatta tgtccgtcaa ccacagctat
    28801 tacacgatga ttaaatatgt caaactatat tatctgtaca aatccggcgg aaaattttaa
    28861 atggaaaaag aattcaggat attaaatatc gtatcggcca agatttgggg tggaggcgaa
    28921 caatatgtct atgatgtttc aaaagcattg gggcttcggg actgcacaat gtttaccgcc
    28981 gtcaataaaa ataatgaatt gatgcacagg cgattttccg aagtttcttc cgttttcaca
    29041 acgcgccttc acacgctcaa cgggctgttt tcgctctgcg cacttacccg ctttatccgg
    29101 aaaaaccgca tttcccacct gatgatacac accggcaaaa ttgccgcctt atccatactt
    29161 ttgaaaaaac tgaccggggt gcgcctgata tttgtcaaac ataatgttgt cgccaacaaa
    29221 actgattttt atcaccgcct gatacagaaa aacaccgacc gctttatttg cgtttcccgt
    29281 ctggtttacg atgtgcaaac cgccgacaat ccctttaaag aaaaataccg gattgttcat
    29341 aacggtatcg ataccggccg tttccctccc tctcaagaaa aacccgacag ccgttttttt
    29401 accgtcgcct acgccggcag gatcagtccg gaaaaaggat tggaaaacct gattgaagcc
    29461 tgtgtgatac tgcatcggaa atatcctcaa atcagactca aattggcagg gcacggacat
    29521 ccggattata tgtgccgcct gaagcgggac gtatctgctt caggagcaga accatttgtt
    29581 tcttttgaag ggtttaccga aaaacttgct tcgttttacc gccaaagcga tgtcgtggtt
    29641 ttgcccagcc tcgtcccgga ggcattcggt ttgtcattat gcgaggcgat gtactgccga
    29701 acggcagtga tttccaatac tttgggcgcg caaaaggaaa ttatcgaaca tcatcaatcg
    29761 gggattctgc tggacaggct gacacctgaa tctttggcgg atgaaatcga acgcctcgtc
    29821 ttaaaccccg aagcaaaaaa cgcactggca acggcaggac atcaatgcgt cgccgcccgt
    29881 tttaccatca accataccgc cgacaaatta ttggatgcaa tataagccgc tttcagacgg
    29941 cctatgccgt ctgaaagcct ttgatgcaac aaaccgccaa attatattcg ttcattggaa
    30001 agaaacaccc cgaatccatc cttcaaaata agaaaatccc aatatctccc aatattacgc
    30061 agcctattgg caaagttttg cagcgtcttc cccggcttgt gctgccgcat caagtgcttt
    30121 gttacaatgt atagtgga
//
LOCUS       DAXHNE010000009        28008 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.009, whole genome shotgun sequence.
ACCESSION   DAXHNE010000009 DAXHNE010000000
VERSION     DAXHNE010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28008
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.009"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(111..422)
                     /locus_tag="ACFM4Z_000300"
     CDS             complement(111..422)
                     /locus_tag="ACFM4Z_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HFC6020402.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
     gene            951..3230
                     /gene="nrdA"
                     /locus_tag="ACFM4Z_000301"
     CDS             951..3230
                     /gene="nrdA"
                     /locus_tag="ACFM4Z_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFC6020403.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKEARLGYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRAEIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            3288..4301
                     /gene="dcm"
                     /locus_tag="ACFM4Z_000302"
     CDS             3288..4301
                     /gene="dcm"
                     /locus_tag="ACFM4Z_000302"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFC6020404.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KNE"
     gene            4294..5481
                     /locus_tag="ACFM4Z_000303"
     CDS             4294..5481
                     /locus_tag="ACFM4Z_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HFC6020405.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            5542..6675
                     /gene="nrdB"
                     /locus_tag="ACFM4Z_000304"
     CDS             5542..6675
                     /gene="nrdB"
                     /locus_tag="ACFM4Z_000304"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFC6020406.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPITVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            6776..6892
                     /locus_tag="ACFM4Z_000305"
                     /pseudo
     CDS             6776..6892
                     /locus_tag="ACFM4Z_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            6909..7199
                     /gene="yfaE"
                     /locus_tag="ACFM4Z_000306"
     CDS             6909..7199
                     /gene="yfaE"
                     /locus_tag="ACFM4Z_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I ribonucleotide reductase maintenance
                     protein YfaE"
                     /protein_id="HFC6020407.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLVGEDEGLSDSLSK"
     gene            complement(7240..7518)
                     /gene="ftsB"
                     /locus_tag="ACFM4Z_000307"
     CDS             complement(7240..7518)
                     /gene="ftsB"
                     /locus_tag="ACFM4Z_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFC6020408.1"
                     /translation="MKWVTVVLSVSLVCCQYSLWFGKGSIGRNNNLKNQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(7532..8818)
                     /gene="eno"
                     /locus_tag="ACFM4Z_000308"
     CDS             complement(7532..8818)
                     /gene="eno"
                     /locus_tag="ACFM4Z_000308"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFC6020409.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMTLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLIVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTSAEFAEYLEGLV
                     NEFPIVSIEDGMDENDWEGWKLLTEKLGSRVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAAYYPGKAAFYQLGK"
     gene            complement(8865..9305)
                     /locus_tag="ACFM4Z_000309"
     CDS             complement(8865..9305)
                     /locus_tag="ACFM4Z_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HFC6020410.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIVAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANRFLITPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSIVFMATALLSAVLSWRLSGKDAV"
     gene            complement(9320..10162)
                     /gene="kdsA"
                     /locus_tag="ACFM4Z_000310"
     CDS             complement(9320..10162)
                     /gene="kdsA"
                     /locus_tag="ACFM4Z_000310"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFC6020411.1"
                     /translation="MDIKINDITIGNNSPFVLFGGINVLESLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVVAMAKTGNVVNIKKPQFLSPSQMKNIVEKFHEAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            complement(10184..10567)
                     /gene="panD"
                     /locus_tag="ACFM4Z_000311"
     CDS             complement(10184..10567)
                     /gene="panD"
                     /locus_tag="ACFM4Z_000311"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFC6020412.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTISGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            complement(10716..14855)
                     /gene="mfd"
                     /locus_tag="ACFM4Z_000312"
     CDS             complement(10716..14855)
                     /gene="mfd"
                     /locus_tag="ACFM4Z_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFC6020413.1"
                     /translation="MTYPIPKPREKSRWPNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKSDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTETQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKEHALPDLAVNRQSDEPLQALKDFQTAFEGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNPLPSPLPQEREQSAAVVSDSLKAAAVSTESRLPPGKSNLHGQIQQQPTPSP
                     VGEGWGEGKAVAAQTEFSAAAISPLPSPLPSPLPQEREQSAAAVSDGLKAKSSLHLVA
                     SNLHGQIRQQPTPSPVGEGWGEGKAVAAQTEFSAAATNPLPSPLPQEREQSAAVVSDS
                     LKAAAVSTESSLPPGKSNLHGQIQQQPAPSPVGEGWGEGKAVAAQSAIAVITESDLYQ
                     YVARSRVHNRRKKHAAVSDGLLRDLAEINIGDPVVHEEHGIGRYMGLVTMDLGGETNE
                     MMLLEYAGEAQLYVPVSQLHLISRYSGQAHENIALHKLGSGAWNKAKRKAAEKARDTA
                     AELLNLYARRAAQSGHKFEINELDYQAFADGFGYEETEDQAAAIAAVIKDLTQAKPMD
                     RLVCGDVGFGKTEVALRAAFVAVMGGKQVAVLAPTTLLVEQHAQNFADRFADFPVKVA
                     SLSRFNNSKATKAALEGMADGTVDIVIGTHKLVQDDIKFKNLGLVIIDEEHRFGVRQK
                     EQLKRLRANVDILTMTATPIPRTLSMALEGLRDFSLITTAPSRRLAVKTFVKPFSEGS
                     VREAVLRELKRGGQVFFLHNEVDTIENMRERLETLLPEARIGVAHGQLRERELEQVMR
                     DFLQQRFNVLLCSTIIETGIDIPNANTIIINRADKFGLAQLHQLRGRVGRSHHQAYAY
                     LLTPEYITKDAEKRLDAIAAADELGAGFTLAMQDLEIRGAGEILGEGQSGEMIQVGFT
                     LYTEMLKQAVRDLKKGRQPDLDAPLGITTEIKLHSPALLPESYCPDIHERLVLYKRLA
                     VCETVQQINAIHEELVDRFGLPEQPVKTLIESHHLRLMAKELGIDAIDAAGEAVTVTF
                     GKNNNVDPTEIILLIQNDKKYRLAGADKLRFTAEMENIEVRINTVKNVLKTLQNRCLP
                     K"
     gene            15040..16467
                     /locus_tag="ACFM4Z_000313"
     CDS             15040..16467
                     /locus_tag="ACFM4Z_000313"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC6020414.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQVAQGLEMIFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLGRYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AKHEDAAACLLNDIRKDILDCRYCG"
     gene            16458..16760
                     /locus_tag="ACFM4Z_000314"
     CDS             16458..16760
                     /locus_tag="ACFM4Z_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020415.1"
                     /translation="MRIAGKYGGRQCRLKGEVPLDGIFGGGRQISGGAKGNARHSAKP
                     PIAADTAFRAAPLRRFPTYPICSEHIYEKEKNTAAGNLFGGFVCLYGDGGTPAPGK"
     gene            16669..17778
                     /gene="mltB"
                     /locus_tag="ACFM4Z_000315"
     CDS             16669..17778
                     /gene="mltB"
                     /locus_tag="ACFM4Z_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFC6020416.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRANEAQAPRADEMKKES
                     RPAFDAAAVFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKAD
                     IVKIMHRPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAV
                     IGIETNYGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGS
                     YAGAMGMPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILV
                     SATLAPGADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEY
                     YLGLNNFYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(17901..19907)
                     /locus_tag="ACFM4Z_000316"
     CDS             complement(17901..19907)
                     /locus_tag="ACFM4Z_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFC6020417.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGSLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRLPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            20196..22223
                     /locus_tag="ACFM4Z_000317"
     CDS             20196..22223
                     /locus_tag="ACFM4Z_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFC6020418.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLGLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGNLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(22314..23867)
                     /locus_tag="ACFM4Z_000318"
     CDS             complement(22314..23867)
                     /locus_tag="ACFM4Z_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HFC6020419.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPAGEMAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGIPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(24116..25309)
                     /locus_tag="ACFM4Z_000319"
     CDS             complement(24116..25309)
                     /locus_tag="ACFM4Z_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFC6020420.1"
                     /translation="MNPKHLIAFSALFAATQANALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLRSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRSAAADFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLTIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILSYGTNEAFGDNID
                     IADTEQKWLDTVRQIHDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(25306..26289)
                     /gene="patB"
                     /locus_tag="ACFM4Z_000320"
     CDS             complement(25306..26289)
                     /gene="patB"
                     /locus_tag="ACFM4Z_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan O-acetyltransferase PatB"
                     /protein_id="HFC6020421.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGVALQENAYALSDGIKAFLSGETPPTAQDGGSADMPSEAAASEAVPQTGET
                     EWKQDTEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPK
                     FFDWPKTIEETLQKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAARAHHVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLEGKIILIPTAHTLSGGEDR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLATKIMEKIVFEPSTQPSSTQP"
     gene            complement(26300..27736)
                     /locus_tag="ACFM4Z_000321"
     CDS             complement(26300..27736)
                     /locus_tag="ACFM4Z_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HFC6020422.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIIVLYSSCVYLLGELLRSDREGTRRFWLGCGIAASLTVLGFFKYFDFFRPLI
                     AQYAGKGGAIDILMPLGLSYYTFQSLAYLVYCFRAPHAARFGWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTHRPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQFNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWLITFHFVCLSFVVFNTANPD
                     DAGAVFSALFANAGGWNAPQRADMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            complement(27865..>28008)
                     /locus_tag="ACFM4Z_000322"
     CDS             complement(27865..>28008)
                     /locus_tag="ACFM4Z_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HFC6020423.1"
                     /translation="NVGIGGGALLGHWVTQYSGISCIGVAGMLTAAAGLWVCLNLNRY
                     IRT"
BASE COUNT         6452 a         6709 c         7962 g         6885 t
ORIGIN      
        1 taaggctgcg gcaacgggag gacctcaaac gaaatggcgt aaggatagtt tgttgtatct
       61 atggaaaatg ccgtctgaac ctgtgttcag acggcatatt ttatatggga ttaacggcgg
      121 aaactgccgc cgtcgaaatc atcgaaagcc tcataagccg cccggcattg gattttctgt
      181 tccgcataag gcagggagcg gaatgctttg cgcgcctttt tgttttcgcg caattcctgc
      241 acgttgcctt gcgatcggta ccacgcggca cggcgttcca aatatttcct gcattcgggg
      301 tgcagcatct gcttgtttgc cttgcggata tgtttggtat cgcggtgggc gggttctgac
      361 gtaccggcag cggcagttgt cgataccagt cccaatattg cagacaacag tatggcaagc
      421 atctttttga cagacatgac gatcctttca gtttgtcatt ctttccagta taggatggaa
      481 ggatggaacg ggcaaattgt cggcagtttc agactcattc agccgacaaa acttgccgtt
      541 tgatatgcag attcaaataa cacattaatc tcaataataa attccaatcg gtaagaaaat
      601 ggaatttgtc ggcggcggcg cggcaaaatc atactttgca aaatttaaca atttgcaggg
      661 gcagaaaaca ggaagctttc ctttttcgtc ggaaaatcct tatttcaccg ccttgtagcc
      721 ggagccggtc aaaaggcaaa aaatttaccc gttttttatc ggtaaagaat tatcagataa
      781 aacaaatatt ataggaaaaa tacgacaggc gggttttatc gcgcattgcc tgaaactgaa
      841 aaatacaacc gttgtcaaga ctggagaaaa tgccaaaaat ccactatatt gtctgcctta
      901 atttatttga aaagactgtg tcttgaatat caagagtgga agaggaagcg atgaatacac
      961 cgactgattt gaaagtaacc aaacgagacg gaagattaga agccattgat ttggataaga
     1021 ttcaccgtgt cgtcacttgg gcggcggacg gattggaaaa tgtttccgtg tcgcaggtcg
     1081 agttgaaatc gcacatccag ttctacaacg gcatccgcac cgacgacatc cacgaaacca
     1141 tcatcaaagc cgctgccgat ttaatttcgg aagatacccc ggactaccaa taccttgccg
     1201 cgcgtttggc gattttccat cttcgtaaaa tagcctacgg cgagtacgag ccgccgcacc
     1261 tttacgacca cgttaaaaaa cttaccgatg ccggaaaata cgacaggcat atccttgagg
     1321 attacagccg cgaagaattt gacgaactga acgcctatat cgaccacgaa cgcgatatgt
     1381 ccttttccta tgccgctgtc aaacagctcg aaggcaaata tctggtacag aaccgcgtta
     1441 cccgccaaat ttacgaaacg ccgcagtttt tatatgtttt ggtggcgatg tgccttttca
     1501 gcaaataccc gaaagaggcg cgcttgggtt acgtcaaacg gttttacgat gccgtttcta
     1561 catttaaagt atcgctgccg actccgatta tgagcggcgt gcgtacgcct acgcgccagt
     1621 tctcaagctg tgtgctgatt gaatgcgacg atagtttgga ttccatcaat gccactacca
     1681 gcgcgattgt gaaatacgtt tcccagcgtg cgggcatcgg catcaatgcc ggacgtatcc
     1741 gcggtttgga cagcgaaatc cggggcggcg aagcgcggca taccggctgc attcccttct
     1801 ttaaaatgtt tcaggcggcg gtcaaatcct gttcgcaagg cggcgtgcgc ggcggcgcgg
     1861 caaccttgtt ctaccccttg tggcatatcg aagccgaaag cctgctggtg ttgaaaaaca
     1921 accgcggtgt ggaagacaac cgtatccgtc agcttgatta cggcgtgcaa atcaaccgcc
     1981 tgctgtacac ccgcctgatt aagggcggca acattacgct gttttcgccc aacgaggttc
     2041 cgggattgta cgaagcgttt tttgccgacc aagacgaatt tgagcggctc tatacgaaat
     2101 acgagcaaga ccctgatatc cgcaagcgca tcattccggc tgccgacctg ttttccacgc
     2161 tgatgcagga gcgtgccgga accgggcgca tctacattca aaacgtcgat cactgcaata
     2221 cgcacagccc gttcgatccg cgcgtcgcgc ctgttcatca gtccaacttg tgtatggaaa
     2281 tcgccctgcc gaccaaaccg ctggacaata tcaacgatcc gaacggcgaa atcgccctgt
     2341 gtacactgtc tgcctttaac ttgggcgcat taaacagctt ggacgaattg gaagggcttg
     2401 ccgacttgac cgttcgtgcg ctcgatgcac ttttagatta tcagggatat ccggtagaag
     2461 ccgcgcgtac ctctactatg ggccgccgtt cgctcggcat cggcgtgatt aactatgctt
     2521 attatctggc gaaaaacggt gtccgctaca gcgacggttc cgcgctcggt ctgacccacc
     2581 gtacctttga agccatacag tattacctgc tcaaagcatc ggcaaacctt gccaaagaat
     2641 acggtgcgtg cacgctcttt aaccaaaccg tttattcgca aggcaaactg cccatcgaca
     2701 cttacaaaaa agatttggat gccgtctgcg gcgagccttt gcattacgac tgggaaagcc
     2761 tgcgtgccga aatcgtcaaa tacggcctgc gcaactctac tctgaccgcg ctcatgccgt
     2821 ctgaaaccag ctctcaaatc gccaacgcca ccaacggcat cgagccgccg cgcggattgg
     2881 taacggtcaa agcatcgaaa gacggcattt tgaaacaagt cgtgccggag tttgaaaccc
     2941 tgaaaaatgc ctatgaaacc ctgtggcagc ttcccggcaa cgaaggctac ctgaaacttg
     3001 tcggcgtgat gcaaaaattc gtcgatcaat cgatttccgc caataccgcc tacgacccgg
     3061 gcaaattcga aggcggcaaa gtttctatga aacaaatgct caaagacctg ctgaccgcct
     3121 acaaatacgg cgtcaaaacc ctgtactacc ataacacccg cgacggtgcg gacgatacgc
     3181 agaccgatat tcaggatgac ggctgcgcgg gcggggcttg taagatttga tgaaaggggg
     3241 agttttcaga tggcctttag attaataaat catctgaaat ataaaatatg aaaaataaaa
     3301 tacaaaatca attagataat atagttacct taaataatga gaaatttact tttattgatt
     3361 tatttgcggg aataggtggt tttcgcattg caatggagaa tgttggtggg cgatgtgtat
     3421 tttctagtga atgggatgat aaagcccgtc aaacctacca agtaaatttt aatgatattc
     3481 cttatggaga tattacatta aaggaaacca aagcagctat tccaagtaag tttgatgtat
     3541 taacagcagg attcccgtgt cagccattct ctatagctgg tgtttcaaag aaaaaaagcc
     3601 taggacgaga aacaggcttc ttagataagg cgcagggaac tctatttttt gatgttgctg
     3661 aaattattgg aaaacatcga cctaaaattt ttcttttaga gaatgtgaaa aaccttgttt
     3721 cgcatgacaa aggaaataca tttaaagtaa ttaaagggac tttagaagag cttgactatc
     3781 agatatttta tcaagttatg aatgcaaaat attatgttcc tcaaaatagg gagcgtattt
     3841 ttattgtagg ttttgataga caatatttta ataaggaaat aaatttcaat tttccttccc
     3901 caccagaatc acaaccaaaa ttaaagcaaa ttttggaaga tgatgtagat aattctttta
     3961 ctctttctga taacttatgg ctttaccttc aaaattacgc taaaaaacat aaggcaaagg
     4021 gtaatggatt tggttttgga ttagttgatt tagatggaat atcacgaact ctatctgcac
     4081 gatattacaa agatggttcc gaaatactca tccctcagaa aggaaaaaat cctaggaagc
     4141 tgacacctag ggagtgctcg cgtttaatgg gatttcctaa agattttgtt attgatgcag
     4201 tatccaagac agctgcatac aagcagtttg gcaattcaat tgctgtaccg ttggttcaag
     4261 ctattgctaa acaaattata aatgagttaa aaaatgaatg acttggcagc tcagacaata
     4321 caagtagtaa ataaatccga tgacttagta gcttcagcaa tacagacagc aaataaatca
     4381 attgcagtat actgtcgtta tattcgtccc aatgatgttg gtactactgg tagtcaccaa
     4441 tcaggatttt atattcaaaa acatttttca gacgacctct ttgatgtagt ttgccaaaag
     4501 ggaacaaata aaactatttc aatcaaaatt aattggcaag atggaagcgt cactaatagc
     4561 aattttaaat attacggcca aggcacgaga aatgaggcga gaattacggg ttttggcaag
     4621 aattttgaat ttttaagtga taaatatagt ggttccttat tggtattgtg cagagcttgt
     4681 tataaagatt tattattcca tgcttttgtt ttatcttcag atgaagatat tgaaatattt
     4741 atcgcagaaa ctaacatttt accaggaagt ttatatttac ctaaaaaaca agaagtagag
     4801 gataacctaa caaaattatt ttcactattt cctaacttcc ccaaaactga agaaatggca
     4861 gttttggcaa gggaaaatat tgcattaaat aaaactaacg ttagtaatgt gttaaaacaa
     4921 tgggtttcaa aggaatatga attattttcc atttttgaaa aacgtgaatt tgaaattatt
     4981 aaatcaaaaa ttactgattt ggatagcttc attaattttg cacattcttt tacaaatcgc
     5041 agaaaagcaa gagcaggaaa atctttagag ttacatttat caagaatatt tgacgaattt
     5101 tccttaaaat ttgagacgca agcgaagaca gaagggaaaa agaaaccaga ttttttattt
     5161 cctggaagtg aagagtacca tgcgatggat gaatcaggga atttcatatt tcaaactgaa
     5221 aagctgacaa tgctaggttc aaagactact tgtaaggatc gctggcgcca agttttaaat
     5281 gaagcagatc gaattcctca taagcattta tttactctac aagaaggtat atctgatact
     5341 caaattcagg aaatgagtga tgagaattta accttagttg tccctaaaga atccgtaaaa
     5401 acatttggaa cttttgggaa aacccatgtt ttaactttag aaaattttat taaatatata
     5461 aagtctcagc aggttagtta aaacttacag atcattttta attaatatag agaatagatt
     5521 atgtcctgcg aacacttaac tatgtcatac agcacctttc ccaaaaccaa aaacgacgcg
     5581 ctgaatgagc cgatgttttt tggtcagccg gttaatgttg cccgttatga ccagcagaaa
     5641 tacgaagtat ttgaaaaact gattgaaaaa caattgtctt tcttctggcg gcctgaagaa
     5701 atcgatgtct cgcgcgaccg tatcgactac gccaatctgc ccgaacacga aaaacatatt
     5761 ttcatcagca atctgaaata ccaaacactg ctcgattcca tccaagggcg cagtccgaat
     5821 gttgccttgc tgcctttggt ttcgattccc gagttggaaa cgtggattga aacgtggagc
     5881 ttcagcgaaa ccatacactc gcgcagctat acccacatca tccgcaatat tgtgaatgat
     5941 ccgtcggtcg tgtttgatga tattgtcgaa aacgaataca ttaccgcccg cgccgaagac
     6001 attgcctgct attacgacga cttaatcgaa tacacccagt attacaatct gttgggcgaa
     6061 ggggtgcaca atgtcggcgg aaaacccatt actgtgtctt tgcgcgggtt gaagaaaaaa
     6121 ctctatctct gcctgatgtg cgtcaacgtg ttggaagcta tccgtttcta tgtttcattc
     6181 gcctgctcgt ttgcttttgc cgagcgcgag ttgatggaag gcaacgccaa aatcatcaaa
     6241 ctgattgccc gcgacgaagc cctgcacctg accggcacgc agcatatgct taatctgatg
     6301 cgttcgggtg ttgatgattc tgaaatggca gaaattgccg ccgagttgca ggacgaatgt
     6361 ttccaactct tcaaaaaagc ggcggaacag gaaaaagaat gggcggcata tttgtttaaa
     6421 gacggttcga tgattggttt gaacaaagaa atcttatccc aatacgtcga atatattacc
     6481 aatctgcgta tgcaggcggt ggggttgccg gccggatttg aaggcgcaaa tcaaaacccg
     6541 attccgtgga ttaatgcgtg gctgtcgtcc gacaacgtac aggtcgcgcc gcaggaagtg
     6601 gaaatatcct cttatttgat cggtcagata gattctgaag tgaatacgga tgatttgggc
     6661 gattttgagt tgtaatgttt tttgaaaaaa tgccgtctga agccggatgt ttcagacggc
     6721 atttgtatgt tgtatgatgg gaaaaacagc gggtaggatc ggttattgtt ccttttatag
     6781 tggattatag tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacaaat
     6841 agtacggcaa agcgaggcaa cgccgtactg gtttaaattt aatccactat atttttgatt
     6901 ggtcggaaat ggcgcgcatt ggtacgaata agggtttttt tgaactcttg gagggcgaga
     6961 ctctgttgga aggcttggaa cggacggggc atatggtgga atatcagtgc cgaagcggat
     7021 attgcggatc gtgccgggtg aagattttgg agggaagcgt tacttatcgg gagccgcctt
     7081 tggctttttt ggggcgggac gagatactgc cgtgctgctg ttgcgtcgag ggggatgtca
     7141 ggctggattg cggtttggtg ggggaggatg aggggctgtc tgacagtttg tcgaaataaa
     7201 agccgtccga acattggttc ggacggcttt ttgacggtat taccggttat gcctgatgag
     7261 tcggtaaaag gtttcgccgt cttggatata acccagttct acccgggcga tttccgaaat
     7321 ggcttcttga ccgttttcca aatcatagac ttcggctgca agggaatggt tgcgtagggc
     7381 gagtgtctgg tttttttctt cctgaacggc aatctgattt ttcaggttgt tgttgcgccc
     7441 gatactgcct ttgccgaacc agaggctgta ctggcagcag acaagtgaga cggataaaac
     7501 gacagttacc cacttcatac gttaaccttt tttatttgcc cagttggtag aatgcggctt
     7561 tcccagggta gtaggcggct tccgccaatt cttcctcgat acgcagcagt tggttgtatt
     7621 ttgccatgcg gtcggaacgg gacagggagc cggttttgat ctgcatacag ttggtggcta
     7681 ctgccaagtc ggcaatggta ctgtcttcgg tttcgccgga gcggtggctc attacgctgg
     7741 cgtagcggtt acgtttggcc aagtcgacgg ctttcagggt ttcgctcaaa gtaccgattt
     7801 ggttgacttt gaccaatagt gcgtttgcca cgcctttttc gatgccttcg gccaagattt
     7861 ttggattggt tacgaacaag tcgtcgccga ccaattgaac tctgctgccc aatttttcgg
     7921 tcagcagttt ccagccttcc cagtcgtttt catccatgcc gtcttcgatg gaaacgattg
     7981 ggaattcgtt aaccaagcct tccaagtatt cggcaaattc tgcgctggtg taagagcggc
     8041 cttcggcttc caagtggtat ttgccgtctt tgtagaattc gctggatgcg cagtccaggg
     8101 cgaacaatac gtcttcgcct gctttatagc ctgcggcttc agttgcttcg acgatcagtt
     8161 gcagggcttc tttgtggctg ttcaggttag gtgcgaaacc gccttcgtca ccaacggtag
     8221 ttgggaagcc tttgctgtcg cacagttttt tcagtgcgtg gaagatttcg gcaccgcaac
     8281 gcagggcttc gcggaaagat tttgcgccga caggcataat catgaattct tggatattca
     8341 ggctgttatt ggcatgttcg ccgccgttga taacgttcat catcggaaca ggcagggtca
     8401 ttgggcctgc gccgcccaag tagcggtaaa gcggcaggcc tgagtcttcg gcagcggcgc
     8461 gtgcaaccgc catagaaacc gccaaagtcg cattcgcacc caaattgcct ttgttttcag
     8521 taccgtccaa ttcgatcatg atttggtcga tataagattg ctcgttggca tcgataccga
     8581 tgagggcttg ggcgatttgg ttgttgacgt gttcgaccgc cttcaatacg cccttgccgg
     8641 aatagcggga tttgtcgccg tcgcgaagtt ccaaagcctc tttttgaccg gtggacgcgc
     8701 cgctcggtac ggctgcgcgt cccattacgc cggattcgag caatacatca cactcgactg
     8761 tggggttgcc gcgtgagtcc aaaatttcgc gggcgaaaat atcaacgatt gcgctcatga
     8821 atgttctcca agtgaagtga agggaggtaa aaaatgggag ggcttcagac ggcatctttg
     8881 ccggaaagcc gccaacttaa aactgctgaa agtagggcgg ttgccataaa aacaatactg
     8941 gaaatgccgt gccacattcc gaaggatccg cctacaaacg acaacagcca attttcatgt
     9001 ccgtatttca gtgcctcgat aacgggtgta atcaagaatc ggttggcggc aagggcggac
     9061 aataaaaaaa gcagcagggc ggtttgccgc cgggttaggg cggcaaaggc ggcagccagt
     9121 accgcgcccc aaacggcaag cccgctccaa gagaggatgt cgaacaatac gccggcaatt
     9181 tcgcccgcct gcattttggg cagcattttg aacagcaccg gcgcgacgat ataacccgcc
     9241 ataatctgca taccgagcca caaggttgcg gcatacaggc tgatttttcg gaaagtctgc
     9301 atcataaggc gaaaccgtgt tactcgattg ttaaaatcgg ttgtgatttg attaaatcgt
     9361 ccaatgcttt gatgcggatt aaaaaatctt ctaaaaggtg cagcggcagc gcgctggggc
     9421 cgtcgcattt tgccagtttc ggatcggggt gcgattcgag gaacagaccg gcaaggcggg
     9481 ttgccatgcc tgcaagtgcc aaatccaaag cctgtgcgcg acgaccgccg gatgcggcag
     9541 aaccagcatc gcgggtttgc agggaatggg taacgtcgaa aataaccggc aggttgccgc
     9601 aggtttgttt catcacgccg aaaccgagca tatcgacaac gaggttgtcg tagccgaagc
     9661 tgctgccgcg ttcgcataaa atcagtttcc cgttgccggc ttcgtggaat ttttccacaa
     9721 tgtttttcat ttgagagggg ctgaggaact gaggtttttt gatgttgacg acgttgccag
     9781 tttttgccat ggcaaccact aaatcggtct gccgcgcaag aaaggcggga agctggatga
     9841 catcgcacac ttcggcgacg ggttggcact gatggggttc gtgtacgtcg gtaatgacgg
     9901 ggatgccgaa ctctgctttg actttttcaa aaatctttaa gccttcttcc aagcctacgc
     9961 cgcgataaga atggatggag gaacggtttg ccttgtcgaa agaggcttta aagatatagg
    10021 gaattccgag tttgcgggtt acttcgacgt aatgcgcgca ggtttggagg gtggaatcca
    10081 agctttccaa aacgttgatg ccgccgaata ggacgaaggg cgagttgttg ccgatggtga
    10141 tgtcgttgat tttaatatcc ataatcaatg tccgttttca gggttacagt acggtgtgcg
    10201 gcggctcgta ggagatgatg tcgcggattt tgttgtttcc gtctaccaag acgactttgg
    10261 gctcgtgtgc ggcgatttcg ggttcggaga gttggacgta agacatgatg atgacgatat
    10321 cgcctttctg caccagcctt gccgcagcac cgttcagaca aatcacgccg ctgccgcgtt
    10381 tgcctgaaat ggtataggtt tcaaaacgtt cgccgttgtt gttgttgacg atggcgactt
    10441 tttcgttggg atagatgcct gccgcgtcca acaggtcttg atcgacggta atgctgccga
    10501 cgtagttcaa atcggcttcg gttacggtgg cgcggtggat ttttccgcca agcatagtac
    10561 ggaacatgta tttccttttg gtttgtgtgc ggtctgacgg gcgcgatgcc gtctggaaat
    10621 ctgaatgccg tattatagtg gagtttcctt caaattcata atggcggctt tcggacaagg
    10681 aaaacggtgt ttcagacggc attgcggtgt cggctttatt tgggcaggca tctgttttgc
    10741 aaggttttta aaacgttttt tacggtgttg attctgacct cgatattttc catctctgcg
    10801 gtaaaccgca gcttatcggc gccggcaagg cggtattttt tgtcgttctg aatcagcagg
    10861 atgatttcgg ttggatcgac attattgttt ttaccgaagg ttaccgttac cgcttcgccg
    10921 gccgcatcaa tggcatcgat acccaattct tttgccataa gccgtaagtg gtggctttcg
    10981 ataagggttt tgacgggttg ttcgggcagg ccgaagcggt cgacgagttc ttcgtgtatg
    11041 gcgttgattt gctgcacggt ttcgcagacg gcgaggcgtt tgtagaggac gagacgctcg
    11101 tggatgtcgg ggcagtagct ttcgggcagc agggcggggc tgtgcagttt gatttcggtg
    11161 gtgatgccca gcggtgcgtc gaggtcgggc tggcggcctt ttttgaggtc gcgcacggct
    11221 tgtttgagca tttcggtgta gagcgtgaag ccgacctgta tcatttcgcc ggactgccct
    11281 tcgccgagga tttcgcctgc gccgcggatt tccaaatcct gcatggcgag ggtgaaacct
    11341 gcgccgagtt cgtctgctgc cgcgatggcg tcgaggcgtt tttctgcgtc tttggtgatg
    11401 tattcgggcg tgagcaggta ggcgtaggct tgatggtggc tgcggccgac gcgcccgcga
    11461 agctggtgca gttgcgccag cccgaatttg tcggcgcggt tgatgatgat ggtgttggcg
    11521 ttggggatgt cgatgccggt ttcgatgatg gtggagcaga gcaacacgtt aaatctttgc
    11581 tgcaaaaagt cgcgcatcac ttgttccagc tcgcgctcgc gcagttgtcc gtgcgccacg
    11641 ccgatgcggg cttcgggcag cagggtttcc agccgctcgc gcatattttc aatcgtatct
    11701 acttcattgt gcaggaaaaa cacttgtccg ccgcgtttga gttcgcgcaa tacggcttcg
    11761 cgcacgctgc cttcgctgaa gggtttgaca aaagttttga cggcgaggcg gcggctcggc
    11821 gcggtagtaa tcagcgagaa gtcgcgcaga ccttcgagcg ccatgctgag ggtgcgcgga
    11881 atcggcgtgg cggtcatggt gaggatatcg acattggcgc gcaggcgttt gagctgctct
    11941 ttttggcgca caccgaagcg gtgttcttcg tcgataatca ctaaacctaa gtttttgaat
    12001 ttgatgtcgt cctgcaccag tttgtgcgtg ccgataacga tgtcgaccgt gccgtccgcc
    12061 atgccttcca gcgcggcttt agtggctttg ctgttgttga aacgcgaaag gctagcgact
    12121 ttcacaggga aatcggcgaa acggtcggca aagttttgcg cgtgctgctc gaccagaagc
    12181 gtggtcgggg cgagtacggc gacctgtttg ccgcccatca ccgccacaaa cgcggcgcgc
    12241 agggcaactt cggttttgcc gaagccgaca tcgccgcaca caaggcggtc catcggcttc
    12301 gcctgcgtca aatctttaat cacggcggcg atggcggctg cctggtcttc ggtttcttcg
    12361 tagccgaagc cgtcggcaaa cgcctgataa tccaactcgt tgatttcaaa cttgtgtccc
    12421 gattgggcgg cacgtcgggc gtaaaggtta agtaactcgg cggcggtatc gcgcgccttt
    12481 tcggcggctt tgcgcttcgc cttgttccac gcgccactgc cgagcttgtg cagggcaatg
    12541 ttttcatgcg cctgaccgga gtagcggctg attaaatgca gttgcgaaac aggcacataa
    12601 agctgcgctt cgcctgcgta ttcgagcaac atcatttcgt tggtttcgcc gcccaagtcc
    12661 atcgttacca agcccatata ccgcccgatg ccgtgttctt cgtgaacgac ggggtcgccg
    12721 atgttgattt cggcaaggtc gcgcaacaga ccgtctgaaa cggcggcgtg tttcttgcgg
    12781 cggttgtgga cgcgcgaacg ggcgacgtat tggtagagat cggattcggt gatgacggcg
    12841 atggcacttt gagcggcaac tgctttgccc tctccccaac cctcccccac gggggagggg
    12901 gcaggttgct gttggatttg cccgtgcaga ttacttttac cggggggcag gctgctttcg
    12961 gttgaaacgg cggctgcttt cagactgtct gaaacgacgg ctgcactttg ttccctctcc
    13021 tgtgggagag ggctagggag agggtttgtt gcggctgcgg aaaattcggt ttgagcggca
    13081 actgctttgc cctctcccca accctccccc acgggggagg gagtaggttg ctgtcggatt
    13141 tgcccgtgca gattacttgc gacgagatgc aggctgcttt tggctttcag gccgtctgaa
    13201 acggcggcgg cactttgttc cctctcctgt gggagagggc tagggagagg gctagggaga
    13261 gggcttattg cggctgcgga aaattcagtt tgagcggcaa ctgctttgcc ctctccccaa
    13321 ccctccccca cgggggaggg agtaggttgc tgttggattt gcccgtgcag attactttta
    13381 ccggggggca ggcggctttc ggttgaaacg gcggctgctt tcagactgtc tgaaacgacg
    13441 gctgcacttt gttccctctc ctgtgggaga gggctaggga gagggtttgt tgcggatgcg
    13501 gaaaattcgg tttggtcggt aaccgcatcg ccttctcctt cggaggcgga agtagtttgc
    13561 tgttggttcg gcgattgcag tccgcccagt ttgaacccgt atgccaacgg cgcaactgta
    13621 atcatcagcg gctcatgcgc cgacaagaag ccctgccagt cggacacagg tttggctttc
    13681 aaaccgtttt gctgcaagaa accgagcatg gtttcgcgcc gtcccaaact ttcggcgcac
    13741 agcaaaatcc gtccttcaaa cgccgtctga aaatccttca atgcctgcaa aggctcgtcg
    13801 gattgtcggt tgacggcaag gtcggggagg gcgtgttcct tgccggaaac atcgggcagc
    13861 acttgtccgt agtttttcag acggcctgca aaaacatcag cagagagata caaatgctgt
    13921 ggaagcaaag gcggataggt ttcgtcgccc tgcgccatcg cgtaacggga tttgacatcg
    13981 ctccaaaagc ggtttgcctc ggcgtgaaca tcgcccaaag agacaaacag cgcgtcttcg
    14041 ccgatatagt caaacagcgt ttccagctca ttttcaaaaa acagcggcag ataatattcc
    14101 acgcccgcgc cgaagtggcc gttgctgacg gctttgtaca cagccgcatc gttcggatta
    14161 ccgtcgactt cttctcggaa gcggctgcgg aagatttttt gcgcttcgct gtcggtgggg
    14221 aactcgtgcg ccggcagcag gcggatttcg gaaacaggag aaatggtgcg ttgtgtttcg
    14281 gtatcgaaag ttttgatgct gtcgatttca tcgtcgaaca aatcaatgcg gtacggcatt
    14341 tcgctgccca tcgggaacaa atcgacaata ccgccgcgca cagcgaactc gcctgccgcg
    14401 acaacgtggg aaacatggtt ataacctgca tccaccaaat cgcttttcag acggcctata
    14461 tccaaagtct gccctgtttt cagccaaaac gtgcgccccg ccagaaacgg cacaggcggc
    14521 agcttctgca tcgccgtggc aaccggcacg aacaacacat ccgccgcgcc gcttttaatc
    14581 tgccacaacg ccgacagccg ctccgacacc aaatcctgat gcggcgaaaa acgctcgtaa
    14641 ggcagcgttt cccagtccgg caggaacacc gccgtgtcgt gcggacggaa aaaccgccat
    14701 gccgtctgaa ggcgcaacgc ctgttccgca tcttgggtca gcacgacctt gagccgcttg
    14761 tgcggcagat aacgcgccaa agccaagggc agcgagcctt gcgaaagatt gggccaacgg
    14821 gatttttcac ggggtttggg gatggggtag gtcatagtgc ggcaatcggc ggacagtgat
    14881 gcaaacggtc attttagcac gggagggggc gcgatgccgt ctgaaaggtc ggcggattgt
    14941 tgcccggcgc actttgtggt aaagtttaga aaatcaactc taaactgtgg caggcggttt
    15001 gaccggccgc cgattaatgg gaaccgacag aggaagccga tgatacacac cgaaccgagc
    15061 gcgcagccgt caactatgga cacggctgct tttttaaagc acatcgaatc cgcattccgc
    15121 cgcatttttt cagacggtat cgacctgatg cgatacctgc ccgaagacaa atggcttgcc
    15181 ttgaagcagg cgggtttgct gttgcccttc ctcgacaaaa aacacggcgg gcgcaagggc
    15241 agccagtttg aaattcagga agtcttgcgg attgcggggc attacggcgt gcccgttacg
    15301 ctgcgtaccg gcatcgaagg cgcgctggtg ttgcagccac tgcaagagtt cggcgatgaa
    15361 gcgcaagtcg cgcaaggttt ggagatgatt ttcaaaggcg agggcggcgg tttgggcgtt
    15421 accgaacccg aaacctccgg cgcggcgatt gcacgcgaaa tgcagtctta ctacgaatat
    15481 accgacggac aaaccattta cgtcaacgcc gcgaaatact ggcagggcaa ctcgcaaagc
    15541 gacttcctcc tcgttgccgc caaagagcgc aaaaacggca aactcgccaa agtcatcgac
    15601 ctgctgctcg tccccaaaac atacatccgc tgcgaaaccc tcgcatccga aggcttgcgc
    15661 gccgtccgtt acgccgtcaa ccgcatcgat gccgaaatgc ctgcaaccgc cgtgatgaaa
    15721 ctctcccaga gcgacgctgc cggtttgcgc gcgttccaaa acatctttat ccgcagccgc
    15781 ctgcaactga tcggcatgac gcacggcatt atggaataca tccttgaaaa cctgggacga
    15841 tacgtccgca acgacatcag attcgtcgat tacgaacgcc gcgaaatccg gcgccgccat
    15901 caggtttccg agattcttta ccgctacgtc tgccattccg tttcgcctgt tgcccccgtc
    15961 gcccatcagc tgatggaggc gaacatcgtc aaaaccctcg ccacggaata cacttatgcc
    16021 gccgcgcaaa tgttgcaaaa actcttgggc gcgaagggtt ttgaacgcgg acacaccgcc
    16081 ggcaatatcg ccatcgacat ccgtcccttc acgatttttg aaggcccgaa cgatatgctt
    16141 tatgccgaaa tttacgacca gtttgtccgc gccaccgccg aagaaaaaga agcaggcatg
    16201 aagttggaca aaaaccaaac cctgctcgac cgcctgcaaa ccgatgcccg ctttgccgcc
    16261 gtcgcccgcg actacacttt gcctgaagac atccgcagct tcctgcagga acacaccctg
    16321 accgatgcct gcgccctgca aaaagtcttt atcggcaaaa tcatcgcccg actctttgtc
    16381 ttcgtacagg cgaaacacga agacgcggca gcctgcctgc tgaacgacat ccgcaaagac
    16441 atattggact gccgatattg cggatagcgg gcaaatacgg cggcaggcaa tgccgtctga
    16501 aaggggaagt gcctttagac ggcattttcg gcggcggcag gcagatttcg ggcggcgcaa
    16561 aaggcaacgc gcggcattct gcaaagccgc cgattgcggc ggataccgca tttcgcgccg
    16621 cgcccttgcg ccgctttccc acttacccta tttgttcgga acatatttat gaaaaagaga
    16681 aaaatactgc cgctggcaat ttgtttggcg gctttgtctg cctgtacggc gatggaggca
    16741 cgcccgcccc gggcaaatga agcccaagcc ccccgcgcgg atgaaatgaa aaaagaaagc
    16801 cgccccgcgt ttgacgcggc agccgtattt gacgcggcag ccgtaccggt atccgacagc
    16861 gggtttgccg ccaatgcaaa tgtccgccgt tttgtggacg atgaagtcgg gaaaggggat
    16921 ttttcccggg cggaatggca ggattttttt gacaaagcgg cttacaaggc ggacatcgtc
    16981 aagattatgc accgcccctc cacatcgcgt ccgtggtatg tgttccgcac gggaaattcg
    17041 ggcaaggcga aatttcgcgg cgcgcgccgg ttttatgcgg aaaaccgcgc gcttatcgat
    17101 gatgtggcgc aaaaatacgg cgtgcctgcc gaacttatcg tggcggttat cgggattgaa
    17161 acgaattacg gcaaaaatac gggcagtttc cgtgtggcgg acgcattggc gaccttaggc
    17221 tttgattacc cccgccgcgc cgggtttttc caaaaagaat tagtcgagct tttaaagctg
    17281 gcaaaagaag aaggcggcga tgttttcgcc tttaaaggca gctatgcggg cgcaatgggg
    17341 atgccgcaat ttatgccttc gagctaccgg aaatgggcgg tggattatga cggggacgga
    17401 catcgggaca tatggggcaa catcggcgat gtcgcggcat cggttgccaa ttatatgaag
    17461 cagcacggtt ggcgcacggg cgggaaaata ctggtgtctg caacattggc gccgggtgcg
    17521 gatgttcagg caatcattgg cgaaaaaacc gccctgacgc ggacggtggc ggatttaaag
    17581 gcgtacggca tcatccccgg cgaagagctt gcagatgatg aaaaggcggt tttgttcaaa
    17641 ctggaaaccg cacccggcgt gtttgaatat tatttgggct tgaacaattt ttatacggta
    17701 tggcagtaca atcacagtcg gatgtatgta acggcggtca gggacattgc caattcgctt
    17761 ggcggcccgg gattgtaatg ttttttaaaa tgccgtctga accacgtttc ggttcggacg
    17821 gcatttttgc atccttttat ggggatttcc cgcatcaggg cggtattcgg ggcgaatccc
    17881 gcttcagacg gcattgccgg tcaaggcttg tccgaaggag gatgtccctg ttctttggcg
    17941 ggcgggagga aaagtatgca gggattgctt ttaatctgat tccaaaaact tttaatcaga
    18001 ttgcggatgc tgccgatttt cgtgccgcgc gagcgcaggg cgacgaagag ggcgaggctg
    18061 aagctgacgc agaggttgac caggccgatg gcgaggacgc tgaaaatgcc gagtacgaac
    18121 gtgcccaaac cgacgttgcc gctgacggcg gcatagccga ggttggcgga ggaaaaggcg
    18181 acgtggcgga tgtccagcgg cagcccgagg aggtgtccga aatagccggt catacccaag
    18241 agcatcccga agatgaagtt gccgaccagc gagccgtagt gtttgtggat gtaggcggcg
    18301 aggacgcggc ggggcccggg gcgcatgatt ttacgcagca aggggttgaa gggcaggcgt
    18361 tggcgcaggt tgaggtagtc ggcgcggttg tcgaaaaaac ctgcgatgat gccggagcag
    18421 aacagccaca gacctgcaat ggcggcatac cacagcgtcg ggtaagcgat gatgtctatg
    18481 gatttgaact ggtaggcggc ggtgtgggca tcgagtatgg gcagccggta cagatgggca
    18541 tagccgaacg atatggcgca cgccaaaagg atggcgatgg aaacgttgcc gaagacggcg
    18601 acactttggg agcggcatac gtcgatgagg agcttggcga gtttgttgtc caccgctttg
    18661 ccgccttcgt tgagatcgac ctgttcggca aagctggcgg cagtcatcgc gggctgcttg
    18721 gtggcgacgg tgcagtgcag catatggatg atcataaagc cgatgccgta gttgaaccca
    18781 gccgacaagg aagtgaggaa ggggctgagg ccgagtgagc cgatgcggat tttgagcagc
    18841 gccattaggg cgatgatgat gccgccgcct gccgccgagt agaacatttt gaaatattct
    18901 ttgcggttgc gggtgatgta gtgttcgccg tggtggcttt tgttttcggt aatgctgcgg
    18961 gcgagcatac ggatgctgct gcggcgcagg tggcgggtgc tgtattgttc gaccgccgcg
    19021 tagatgaggg agttcatcag cgcgatggtc aggcggttgc tgttgccggc gccggtttgg
    19081 atgtcggtga gcagcttcag acggtctacg gtctgccgga gccgttcgag caggtgggcg
    19141 actttgaccg acgaaccgga gccggcgccc gtccctttgc ggcgcaaata gtcaatctgg
    19201 ctgaaacatt ggtcgaacat cacttcgagg tgggcggtgt cgtaaggcgt ggtgccgttg
    19261 cggtagtgtt cgaccagttt ggcggtttcg cgttggaggg cgacgaagga agaatcggct
    19321 tccagcaggc gcggggcgat gcggatgagg tcgggttcga tggcttcgga tgccgtccag
    19381 acagacagca tttcgatggc gcgcaaacgc gcatcggcaa ggcggatgcc cgccgtctgc
    19441 aatttggacg gctcggcgtg gctccggatg agttcgtata aaaccagcca ttggcggata
    19501 ttgagcgttt gcagccattt gtcgtcgttt tcggaatgga aaagataaag gaagacttcg
    19561 cccaagttgg caaaatcttt atatgacggg ctgaagcgtt cgtagatgcg tatgcccatt
    19621 tcccgggcaa agctgtggcg cgagaagatg ccgagtttga tgagtgccgg ataaatgtgt
    19681 atttgcgcga gccaaatgta aaaacaccgg ctgaagcggg acagtaattc cctgtcttgt
    19741 ttgaatgtgt cgataatcag gtcgaaacgt tcccccgcac attttttgcc gccacggcgc
    19801 aaaaatttaa tcagtgcgtt gaggacgttg acaaaatcgg tatgtcccaa gctttccgaa
    19861 agcagggggc gcaggttttg aggggtaatt ttcttcatat cagccattgt accgcatcgg
    19921 caatccggcg ttaaacgccc cgatagcggg gagggcgcgc ctgcggtggg cggcgcattt
    19981 tccgccggtt tgaaatcaaa ctatattaat gatataataa atatcgataa atcaatctga
    20041 tagaataatc cgtattgatt tttaattttt tcgattataa caatttcggc cggtttgtgc
    20101 atttcgtcat ccggaagccg gtattttacc ggcaggtttt gaccgctttg cccgatgacc
    20161 gggtttgtct gtttttaaca aaggagtgag aaggtttgtc cctgtctgaa tttatagaac
    20221 gccgaacgtc atttaatccg atggttattt tgacgacttt gttttttgtg tgtgttttgg
    20281 tggtattggt tttaaccgtg ccggatcagg tgcagatgtg gctcgatcgg gcaaaagaag
    20341 tcatttttac cgagttcagc tggttttatg ttttaacgtt ttccattttt ctgggtttcc
    20401 tgctgatact ctcggtcagc agtttgggaa acatcaggct cggacgggat gaagatgtgc
    20461 cggaattcgg cttcctgtcg tggctggcga tgctgtttgc ggccgggatg ggcgtgggtc
    20521 tgatgttttt cggcgtggca gagccgttga tgcattattt ttcggacatt acggccggca
    20581 cgccggaaca caggcagcag caggcattgc tgcacacggt gttccattgg ggcgttcacg
    20641 cttggtcggt gtacggtacg attgcattgg ctttggctta tttcggtttc cgctacaagc
    20701 tgccgcttgc cctgcgttct tgtttttacc ccctgttgaa agaaaaaatt tccggaaggt
    20761 tcggcgatgc cattgatatt atggcgttgc ttgctacttt tttcggcatc atcaccacat
    20821 tggggttcgg ggcttcgcaa ctgggcgccg gattgcagga aatgggctgg attgccgaaa
    20881 acagcttcag cgtgcaggtt ttgattatcg ccgccgtcat gtccctcgcc gtcgtttcgg
    20941 caatatccgg cgtggggaag ggcgtgaagg tgttgagcga gttgaacctg ggccttgcgt
    21001 ttttgctgct gttttttgtt ttggcggcgg gacccactgt ttacctgttg tcggcattcg
    21061 gcgacaacat agggaactac ctcggaaatc tggtgcgcct cagttttaaa acttatgcgt
    21121 acgaacggga acacaagccg tggtttgaat cttggacggt gctttattgg gcgtggtggt
    21181 gttcttgggc gccgtttgtg ggtttgttta tcgcgcgcat ttcaaagggg cgcaccatcc
    21241 gcgagtttgt cttcggggtt ttgctcatcc ccggcctgtt cggcgttttg tggtttaccg
    21301 tcttcggcaa tacggcgatt tggctgaatg acggggttgc ggggggaatg ctcgaaaaga
    21361 tgacctcctc tccggaaacg ctgcttttta aattctttaa ttacctcccc ctgcccgaat
    21421 tgacgagcat cgtcagcctg ctggtcattt ctctgttttt tgtaacttct gccgattccg
    21481 ggatttatgt cctgaacaat attacctctc gggacaaagg cttgagcgcg ccacggtggc
    21541 aggcggttat gtggggcgtg ctgatgtctg ccgttgccgt tttgctgatg cgctcgggcg
    21601 gactcggcaa cctgcagtct atgaccctga ttgtttccct gccgtttgcc ctgctgatgc
    21661 tgataatgtg tttcagcctg tggaaaggct tgagtgcgga taagaaatat tttgagaccc
    21721 gggttaaccc taccagtgta ttttggacgg gcggcaagtg gaaagaacgg ctggtgcaga
    21781 taatgagcca gacgcaggag caggatattt taaaattcct caaacatacc gcatcgcccg
    21841 ctatgcacga gttgcaacgg gagctttcgg aagaatacgg cttgagcgtc cgggtcgata
    21901 agatgtttca tcaggacgag cccgcaatcg agtttgtcat tcgcaaagag acgatgcgcg
    21961 attttatgta cgggattaag tctgtcgggc aggatgtatc cgatcagttg attaacgacg
    22021 gcaacttgcc gcatatccgg caccagacaa cttacaaacc ttacgcttat tttttcgacg
    22081 ggcgcgtcgg gtacgatgtg cagtatatga acaaggacga gctgattgcc gacattttga
    22141 aaaactacga acgttatttg atgttgttgg atgatgtcgg tcaggaactg atggcgcacg
    22201 agcaggtgga attggcagag taagtgcctt atctccggcg tttgccaaaa aaaatgcggt
    22261 gaatcagatt caccgcattt ttttgccgga cggggcttcg gacggcacgg cgttcatttg
    22321 tttccgtcaa actgctcctt caacacccgt ttcaatacct tgcccgtagc gttgcgcggc
    22381 agcccgtctt taaagtggat ttgtttgggg attttgaaat ttgccagcac ggtacgcagg
    22441 tggcggcgga tttcgttctc gcccaaatcc ataccttcct tcaattggac gaaggcgacg
    22501 atttcctcgt cggcataacg gtctttcacg ccgatgacgg cagcggcttc gacggcatcg
    22561 agtttgtaga tttcttcttc gatttcgcgc ggatagacgt tttgaccttt ggaaataatc
    22621 aaatcttttt tgcggtcgac gataaagata aagccgtctt cgtctatggt aacgaaatcg
    22681 cccgttttca accagccgtt gacgatggtt tcatcggtgg cggcaggcat attgaggtag
    22741 ccccgcatca ccgaaccgcc cctgacgatc agttcgccca cttcgccgcg cggcacttcg
    22801 accaattctt catcgacggc tttggcttcc aaaccgggca gggggatgcc gacgctgcgg
    22861 gctttttgcc tctcgggcgt attgacggcg acgacgggag aggcttcgct cagtccgtag
    22921 ccttccagca atttggcgcg ggggaacttg gctttgaaat cgaggatggt ttgttccgcc
    22981 aaaggcgcac cgccgctgat aaacaggcga atgcggttga accatctgaa ataccaaggg
    23041 attttcgcct tgctcattgc ggtgtaaatc gcgggtatgc ccaaaaacac ggtcgcgcgt
    23101 ttgagcaggg cctgtttcaa aacgttggaa aacgggaaaa cggatttgac caaaataatc
    23161 gaacacgcca tataaatcgg cagcagcacc atagccgtca gcgtgaagct gtggaacatc
    23221 ggcaggaaaa cgataaagcg gtcgcgcttg gagattttaa agatgcgttc gatgccgttc
    23281 aggttggcga acaggttggc gtaactgatt agcgcgcctt tgggatgccc cgtcgtgccg
    23341 gaggtgtaga tgatgtgtgc caaatcattt atccggggtt ggcggcccaa gtcgggtttt
    23401 tcggggaagc ggcgcacgtc ttcaaaaaag gcatcgcctt ccgccatttc gccagccgga
    23461 cggcttttgt ccgtccaaat gattttttcg acgggcgttt gcgccttcaa gcccgccaac
    23521 tcttttgaca ggcccgccga ggcgaacagg aagcgcgcct tgcagtcgtt caggatatac
    23581 gcgtattcgc tgtttttcaa aaatgtgttc atcggtacgg cgaccgcgcc gatggcggag
    23641 acggcgaaat aggcggtaat aaattctgtg gaattggaaa ccgccagcgc gaccgtgtcg
    23701 ccgaacttca cgccgatatt ttgcagatac gccgcgacgg cttcggcctc ctgcttgagc
    23761 gcgcggtagg cggttttttc cttgccgtcg aacactgccg tgccgttgcc gtttttgcgg
    23821 caggcggcgg cgagcatttc gtagaaattg gcataagtcc ggttcatgaa acttccttta
    23881 tggttgcgga cggcgaacgc cctcatttga aaacaatatg ccgtatgaaa acggaaggcg
    23941 gcagtttatc atctttgcct tcagacggca tatcaaacgc cgcctttgcg ccgtatgccg
    24001 gaggttcgcc tgcgtctttc caatgtctga ggcgaatgcc gtctgaagca atatgaaggt
    24061 atgcgtttca gacggcatcc ccctgttgcg gaaataccgt ccgcctcctg tccgattatt
    24121 gcctgattgc ggcggcgcgg acgagttctt cgaggctgtc ggcgagcatt tccgccgcgc
    24181 gccggtagcc tttggcggag aagtgtacgc cgtctttggc ggcccatcct tggttgagcc
    24241 agtttttcat gctgcatatg ccgcccatgg cgttttgcca agaccagaac atggtttgtc
    24301 cctgccgggc gacgcgccgc tgcatctgtt ggacttcggt caggcggacg ggacgtgtgc
    24361 cgcatacgcc gagcgtgttt ttcagggatt cgggtgcgcc gatgatgagg atgccggcgg
    24421 caggcaggct gtcgtggatt tggcggacgg tatccagcca tttttgttca gtgtcggcaa
    24481 tgtcgatgtt gtcgccgaag gcttcgttgg tgccgtagga aaggataacc aaatcggcgc
    24541 cggtttgggc aaggtcgttc atccggtcgg cacgccattt cgaccactgg gttaattgtg
    24601 cgccgttgat gcccatcgcg gaaacggtaa tgccgccggc gggattttcg atgttgatga
    24661 agccgatgtc ccacggcatt tcggtctgta tggtcagggg cagtgccgcg cccgtatcca
    24721 gtacctgcca gccgccgccg ttggcggaga cggtgttgcc gttgacggtc agggtttgtt
    24781 cggcgagcag gggtttggca aacagggaaa tgcgctgttt gccggtcttg ccgtccgatg
    24841 cggtcagggt catgctgccg ccgctgccgg tgtgggcgag gatgccaccg agcgggaagt
    24901 ctgctgctgc acttcggctg gtgaggcttt gccagttgcc gttgtgccgg actgccgcca
    24961 tgcgctgccc tttgacgttg gcggggtaga cccagcctat gccgccgtcg ccccatgttt
    25021 tttgcaggcg tttgcggagg ctgtcggtaa agaagtcgcc ggcggtgtgc gagtcgccga
    25081 tttgcaggat gcggaaggtc tcgccgctgc cttgtgcgac ggatcggagt tttttcatcc
    25141 aaggcgaggc ggcggcgttg ccgtagtcgg tcaagagccc gtttgtatcg gtgtaggggg
    25201 cggacgggga aacggtaacg gtgtcgaggc tgacggaggc gacgggcaga gcgtttgcct
    25261 gcgtggcggc gaacagggcg gaaaatgcga tgaggtgttt gggattcatg gctgtgtact
    25321 tgatggttgc gtacttggtt caaaaacgat tttttccatt atttttgtcg ccagcagttt
    25381 ttgtccttcg gcggtaaagt gtatgccgtc cttgctgcgg tagcggacgg gtttgccgtt
    25441 gacgttgacg gagtccgtgt agcggtcttc cccgccgctc agggtgtgcg cggtgggaat
    25501 caggatgatt ttgccttcca ggtgttccga aagcagtttg tctaggtagc gcatctgtcc
    25561 gtcgagcttg gcttttttca tataggggat gccgagccag acgacttgga cgtggtgcgc
    25621 gcgggcggct tcaaggatgc ggtcgacgcg tttcaggtat tcttgcgccc attcgtcgga
    25681 agcgaatttg aggtatcgtt tgccgacggg gaaatcccac gggtcgttcg ggccgaggaa
    25741 gacggcgagc acgctgattt cggggtgttt ttgcagggtt tcttcaatcg ttttcggcca
    25801 gtcgaagaat ttgggatagg aaagccccgt gctttgtttg ctgaggttga cggattcgat
    25861 gccgtattgc tgtttcaggc ttttttgcac gaagggtgca acgccctgca tcagcgagtc
    25921 gccggcgaaa aagactttgt cgccgctgcg gacggcggcg gcttcggtgt cttgtttcca
    25981 ttctgtttca ccggtttgag ggacggcttc ggatgcggcg gcttcagacg gcatatctgc
    26041 cgaaccgccg tcttgagccg tcggcggcgt ttcgccggac aggaaggctt tgatgccgtc
    26101 tgaaagggcg taggcgtttt cttgcaacgc cacaccgctc cgccaccatc cgtaggcggc
    26161 aagcggttcg agcggactgt tgcggtggta ggtctgctgc cagtaggcgt tgatggggtt
    26221 ttggctgaac cacacggcaa tcagggcaga catcagtatg gaggcgaaaa gggaaagaaa
    26281 gtttttcatg tccaaaccct taaaaattgg cataaataaa gccgggtatc cccgaggggg
    26341 cgaggacgat aatcagcagc aggacggcgg aaacggggat aaaccacagc cacatcggga
    26401 ttttttccaa acctttgacc gcgccgtcga aagcgcgttg caggtaaggg tagagcagca
    26461 tcacggatgc aaacgaggca agcagcagca tgtctgcccg ttgcggcgcg ttccagccgc
    26521 cggcattggc aaagagcgcg ctgaaaaccg cgccggcatc gtcgggattg gcggtattga
    26581 agacgacaaa gctaaggcag acgaaatgga aggtaatgag ccatgagagc ggcgcaaggt
    26641 atttcagacg gcatagtgcg ttgcgtccga aatagcggtc gcccgtgttg agcagcacca
    26701 gcgccgtgcc gtgtagtgcg ccccaaatga ggaagttcca gccgtagccg tgccagatgc
    26761 ctgagagcac cattgccgcc atcaggttga actgtgtccg caaaaagcct tttttgctgc
    26821 cgcccaaggg gatgtagatg tagtcgcgta tccaggtgga aaggctgatg tgccatttgt
    26881 cccaaaatgc gcggatattc gcggcacgaa gcggtgcgga gaagtttttg ggcagccgga
    26941 agcccagcag cattgccatt ccgataacca agtcggaata tccggaaaag tctaaaaaga
    27001 gttggaaggt atagccgtac acgcccgcca atacgcccca gccgtcgaat tgggcgggat
    27061 tttcaaatac gggcgacacc cagttttccg ccagcatccc cgccagccac cattttttgg
    27121 caatacccag cagaatcagg gaaacggcga gtgcggggcg gacgggcgaa cgcggtcggt
    27181 gggtacggat ttgcgccaat gcgcctgcct gctcgccgtc tgcgcttttg aatgcggcgg
    27241 cgcggataat cgggccggag gtaacggtgg ggaaaaaact caggtgcagt agcagctcgt
    27301 gccacccgaa acgcgcggcg tgcggggcgc ggaagcagta aacaagatag gcgagcgact
    27361 ggaaggtgta atacgaaagc cccagcggca tcaggatgtc gattgcgccg ccttttccgg
    27421 catattgggc aatcagcggc cggaaaaagt cgaaatattt gaaaaagccc aagacggtca
    27481 gcgaggcggc aatgccgcac cccagccaaa agcggcgcgt accttcgcga tcggaacgga
    27541 gcagttcgcc caaaaggtac acgcaggagg aataaaggac gatgattgcc gcaaatacgg
    27601 ggccgatatg gtagagccag cccataccgg cagccaaaag cagcaggttt tggacggacg
    27661 ggtatttcgc caagccccag taaatcggca ggaaggcgag aaagaacagt gcgaactcga
    27721 cagacagcag cggcataaac catccttgga acaaaaaata ggcgttaaaa cacggcaaag
    27781 cccgttattt taagcagggg tggatatttc ttcaatcaaa atgccgtctg aagggcggga
    27841 ggggcttcag acggcatcgt gcggtcaggt tcggatatag cggttcaggt tcaggcagac
    27901 ccacaaacct gccgccgccg tcagcatacc cgcaacgccg atgcaggaaa tgcccgagta
    27961 ttgcgtaacc caatgcccca gcagcgcgcc gccgccgata ccgacgtt
//
LOCUS       DAXHNE010000010        27738 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.010, whole genome shotgun sequence.
ACCESSION   DAXHNE010000010 DAXHNE010000000
VERSION     DAXHNE010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27738
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.010"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            182..538
                     /locus_tag="ACFM4Z_000323"
     CDS             182..538
                     /locus_tag="ACFM4Z_000323"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HFC6020424.1"
                     /translation="MLSAYFPVFVFILIGLAAGVLFILLGTILGSKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKELGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            529..1011
                     /locus_tag="ACFM4Z_000324"
     CDS             529..1011
                     /locus_tag="ACFM4Z_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HFC6020425.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            1024..1617
                     /locus_tag="ACFM4Z_000325"
     CDS             1024..1617
                     /locus_tag="ACFM4Z_000325"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HFC6020426.1"
                     /translation="MASIQNLYETVVGVLGDQASKVISALGEITVECLPEHYVSVMTT
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVQNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            1607..2863
                     /gene="nuoD"
                     /locus_tag="ACFM4Z_000326"
     CDS             1607..2863
                     /gene="nuoD"
                     /locus_tag="ACFM4Z_000326"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HFC6020427.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELEGETIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVTPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            2863..3336
                     /gene="nuoE"
                     /locus_tag="ACFM4Z_000327"
     CDS             2863..3336
                     /gene="nuoE"
                     /locus_tag="ACFM4Z_000327"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFC6020428.1"
                     /translation="MLSAESLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFIADYIGISPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQR
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            3726..5027
                     /gene="nuoF"
                     /locus_tag="ACFM4Z_000328"
     CDS             3726..5027
                     /gene="nuoF"
                     /locus_tag="ACFM4Z_000328"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFC6020429.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            5027..5464
                     /locus_tag="ACFM4Z_000329"
     CDS             5027..5464
                     /locus_tag="ACFM4Z_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020430.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRGNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINEL"
     gene            5750..5989
                     /locus_tag="ACFM4Z_000330"
     CDS             5750..5989
                     /locus_tag="ACFM4Z_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6020431.1"
                     /translation="MANGKNGTLYIGVTMNLPERVWQHKNHVNIDGFTARYDVHDLVW
                     YQFFENMPEAVAKEKTMKKWRREWKIKLIEEQNTE"
     gene            6301..8562
                     /gene="nuoG"
                     /locus_tag="ACFM4Z_000331"
     CDS             6301..8562
                     /gene="nuoG"
                     /locus_tag="ACFM4Z_000331"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFC6020432.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGSLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIIQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGIWANPMNTVEELYLAKKLAN
                     GLGVKNFATRLRQQDKRLSDDLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELLMPLLSQEAAHPDEWAGRLKNLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELAAATGAVLGILPQAANSVGADVLNVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLSSKLDNRSTWTGEGVQTASDRLVRVGGVGIYHTDAIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVMHLPLHTENAA
                     LGALMDTIELAGA"
     gene            8565..9641
                     /gene="nuoH"
                     /locus_tag="ACFM4Z_000332"
     CDS             8565..9641
                     /gene="nuoH"
                     /locus_tag="ACFM4Z_000332"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFC6020433.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGIVGTPSAFWMFAKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGLWMISPLNLWK"
     gene            9723..10202
                     /gene="nuoI"
                     /locus_tag="ACFM4Z_000333"
     CDS             9723..10202
                     /gene="nuoI"
                     /locus_tag="ACFM4Z_000333"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFC6020434.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            10233..10940
                     /locus_tag="ACFM4Z_000334"
     CDS             10233..10940
                     /locus_tag="ACFM4Z_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020435.1"
                     /translation="MEGLINALKYLAEHEPIDNFEEIRTRNSPIELPSGLSNFEQNIF
                     LKENLSPKLQNDDSLKTHYWIIREWGGIKSFKQSAENSQLIRQFLSELNSGKLSSGLL
                     KISSLSKLASFIDCERFAIYDSRAIFSLNWLLFKFTNADLFFQPQGRNRELEIRNMNV
                     LFHFSDIKPNYRKPDVSFHQYCGLLQDLAKQVYGKQAKPYHIEMLLFKIATTWICADM
                     DQLIKFDCLRNQDFQTA"
     gene            10976..11647
                     /locus_tag="ACFM4Z_000335"
     CDS             10976..11647
                     /locus_tag="ACFM4Z_000335"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFC6020436.1"
                     /translation="MTFQLILFYIFAVIILYGALKTVTAKNPVHAALHLVLTFCVSAM
                     IWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPTDYNNIRDLGSRIYTEYLLPFELAAVLLLLGMV
                     AAIALVHRKTTNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGFKPE
                     GEGKA"
     gene            11644..11949
                     /gene="nuoK"
                     /locus_tag="ACFM4Z_000336"
     CDS             11644..11949
                     /gene="nuoK"
                     /locus_tag="ACFM4Z_000336"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFC6020437.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDGLKG"
     gene            11983..12558
                     /gene="fic"
                     /locus_tag="ACFM4Z_000337"
     CDS             11983..12558
                     /gene="fic"
                     /locus_tag="ACFM4Z_000337"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC6020438.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKVVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            12682..13023
                     /locus_tag="ACFM4Z_000338"
     CDS             12682..13023
                     /locus_tag="ACFM4Z_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020439.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVFPK"
     gene            13072..15096
                     /gene="nuoL"
                     /locus_tag="ACFM4Z_000339"
     CDS             13072..15096
                     /gene="nuoL"
                     /locus_tag="ACFM4Z_000339"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFC6020440.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIVSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHSLGKNDNPHESPLVVTLPLILLAIPSVIIGYLAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQTFRPVYVLFENKYYLDVLYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            15192..16688
                     /locus_tag="ACFM4Z_000340"
     CDS             15192..16688
                     /locus_tag="ACFM4Z_000340"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFC6020441.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLATGSDSRAPFARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLIMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIEQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     QDINCREFAILAILAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            16698..18140
                     /gene="nuoN"
                     /locus_tag="ACFM4Z_000341"
     CDS             16698..18140
                     /gene="nuoN"
                     /locus_tag="ACFM4Z_000341"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFC6020442.1"
                     /translation="MNWSDLNLMPAMPEIVLLSLLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFTFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMFAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGHVWLSVFAVIMSLIGAFYYLRVVKVIYFDVPDHDQPVGSNYAAKFV
                     LTVNAFLLLLWGIMPQTVIDWCAKALENTL"
     gene            18252..18551
                     /locus_tag="ACFM4Z_000342"
     CDS             18252..18551
                     /locus_tag="ACFM4Z_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HFC6020443.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHMIELA
                     AGFALTAVLAYILESRAGSVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(18660..19556)
                     /locus_tag="ACFM4Z_000343"
     CDS             complement(18660..19556)
                     /locus_tag="ACFM4Z_000343"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFC6020444.1"
                     /translation="MNPTNDLKTWQQRAQAQTELLLERFLPSEGEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGEAVHEAVEQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLTGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSRGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGATACPDLSDAELSVLDAYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(19540..19764)
                     /locus_tag="ACFM4Z_000344"
     CDS             complement(19540..19764)
                     /locus_tag="ACFM4Z_000344"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009115303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFC6020445.1"
                     /translation="MKKNAPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDTDGLKELNLESDE"
     gene            complement(20011..20934)
                     /gene="rsgA"
                     /locus_tag="ACFM4Z_000345"
     CDS             complement(20011..20934)
                     /gene="rsgA"
                     /locus_tag="ACFM4Z_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFC6020446.1"
                     /translation="MPSEHPFSDGIPTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIEAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAQTGAASPERLAFLQGITDELPG"
     gene            complement(20939..22795)
                     /locus_tag="ACFM4Z_000346"
     CDS             complement(20939..22795)
                     /locus_tag="ACFM4Z_000346"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6020447.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALIFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPNALPLKVSQGEIKFEHVDFSYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPDLSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            complement(22821..22958)
                     /locus_tag="ACFM4Z_000347"
     CDS             complement(22821..22958)
                     /locus_tag="ACFM4Z_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020448.1"
                     /translation="MPSEGGAAFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            complement(22958..23542)
                     /gene="ruvA"
                     /locus_tag="ACFM4Z_000348"
     CDS             complement(22958..23542)
                     /gene="ruvA"
                     /locus_tag="ACFM4Z_000348"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFC6020449.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHAVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            complement(23624..23950)
                     /locus_tag="ACFM4Z_000349"
     CDS             complement(23624..23950)
                     /locus_tag="ACFM4Z_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC6020450.1"
                     /translation="MNIKHLLLTSAATALLSISAPALAHHDGHGDDDHGHAAHQHNKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(24012..24731)
                     /locus_tag="ACFM4Z_000350"
     CDS             complement(24012..24731)
                     /locus_tag="ACFM4Z_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HFC6020451.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVNQGTAHVKIEQIVPGQSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAVSSSGTNLSVEKRRYEYVVKMGPFTSQ
                     ERAAEAEAQARGMVRAVLTAG"
     gene            complement(24728..24850)
                     /locus_tag="ACFM4Z_000351"
     CDS             complement(24728..24850)
                     /locus_tag="ACFM4Z_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC6020452.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            complement(24861..25325)
                     /gene="trmL"
                     /locus_tag="ACFM4Z_000352"
     CDS             complement(24861..25325)
                     /gene="trmL"
                     /locus_tag="ACFM4Z_000352"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFC6020453.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            complement(25390..26115)
                     /locus_tag="ACFM4Z_000353"
     CDS             complement(25390..26115)
                     /locus_tag="ACFM4Z_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFC6020454.1"
                     /translation="MDFLSCWRRIADAPTIRRCVLCHGSSGVSDGICAGCRNDLAAYR
                     TDAANSCPLCFRHVQGGAVCGGCQKKPPAFDRMWASLHYEPPVSNMIRALKHLADLGM
                     VQPLADLMMQNPPDLLADECFDFVLPVPLSRERLLQRGFNQSEGIVGLLAQRYGWRIL
                     PRHTVFRHHRPPQSTLKGGERRRNIKNAFEIRTPPPKNCNILLIDDVFTTGATLDELA
                     KTLKKSGANRICCWTLARTPMKK"
     gene            26114..26857
                     /gene="bioH"
                     /locus_tag="ACFM4Z_000354"
     CDS             26114..26857
                     /gene="bioH"
                     /locus_tag="ACFM4Z_000354"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFC6020455.1"
                     /translation="MPDAVKKVYLIHGWGANRHVFDDLMPRLPATWPVSAVDLPGHGD
                     APFAQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFAR
                     LTADEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTPDADGIIGRILPDLAR
                     CGTPSALQEALDAAERADARHLLDKIDVPVLLVFGSKDAITPPRMGEYLHRRLKGSRL
                     VVMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            26859..27647
                     /locus_tag="ACFM4Z_000355"
     CDS             26859..27647
                     /locus_tag="ACFM4Z_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC6020456.1"
                     /translation="MNPQDKCWQVHRHLAEYADQRLTLVRNAPKYILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAARKGGFWQRFTGKGVVQHCQSPIAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALKTDGLLFFTCFGRDTFTELKCRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMAWNDENAA
                     RSCVGTIFEREGGLGITLETVYGHAVKKLMLPQGENVVQFFPKR"
BASE COUNT         6161 a         6937 c         7672 g         6968 t
ORIGIN      
        1 cgaattacgg tgtaaaaatt tatatgacct tataaaatca aataagaatc gttatcataa
       61 catgattgta tttattgggt ttttttgggc gttttgccga tatttacctt ttaatggttt
      121 ttgaaattcg ctaaaatacg aaattattgt agaaattttg ttaacggatt tgggtgtaac
      181 catgttgtcc gcttactttc ccgtctttgt ctttatcctc atcggcctcg cggccggcgt
      241 actgtttatc ctgctcggca caattttggg ctcgaaacgt cactatgccg aaaaagacgc
      301 accttacgaa tgcggttttg aagcctttga aaacgcaagg atgaagttcg acgtgcgcta
      361 ttacctcgtc gccatcctct ttatcttgtt tgatttggag gtcgcgttca tgttgccgtg
      421 ggcagtcgtg ttcaaagaat tgggcgcata cggcttctgg tctatgctgg tgtttatcgt
      481 cgttttgacg gtaggctttg tttacgaatg gaaaaaaggt gcgctggaat gggaatagaa
      541 ggcgttttga aaaaaggttt catcaccacc agcgcggata cggtgctgaa ctatatgcgt
      601 accggttcat tgtggccggt tactttcggc ttggcctgct gcgccgtgga aatgatgcat
      661 gcaggtatgg cgcgttatga ccttgaccgt ttcggcatca ttttccgtcc gtccccacgt
      721 caggccgacc tgatgattgt ggcaggtacg ctgaccaaca aaatggcgcc agccctgcgc
      781 cgcgtgtacg accagctcgc cgagccgcgc tgggtattgt ctatgggctc gtgtgccaac
      841 ggcggcggct attatcacta ttcttattcc gttgtgcgcg gtgctgaccg cgtcgtgccg
      901 gtagacgttt atgtgccggg ttgtccgccg actgcggaag ccctgattta cggcctgatt
      961 cagctccaac aaaaaatcaa gcgcacttcc accatcgcgc gtgacgagta aggagaggac
     1021 gatatggcaa gcattcaaaa cttatacgag accgtcgtcg gcgtgcttgg cgatcaggca
     1081 agcaaagtca tttcagcttt gggcgagatt accgtcgagt gtctgcctga acactatgtt
     1141 tcagtgatga ctacgctgcg cgaccatgaa gaactgcatt tcgagcttct ggtcgacttg
     1201 tgcggtgtcg attacagcac ttacaaaaac gaagtatggc agggcaaacg ctttgccgtc
     1261 gtcagtcagc tgctttctgt tcaaaacaat caacgcatcc gcgtacgcgt ctgggtttca
     1321 gacgacgact tccccgtagt cgaatccgtc gtcgatattt acaacagcgc ggattggtac
     1381 gaacgcgaag ccttcgatat gtacggcatt atgttcaaca accacccgga cttgcgccgc
     1441 atcctgaccg attacggctt tgtcggacat ccgttccgca aagacttccc gatttccggt
     1501 tatgtggaaa tgcgttacga cgaagagcaa aaacgcgtga tttaccaacc tgttaccatt
     1561 gagccgcgcg aaatcacgcc gcgtatcgtc cgtgaggaga actacggtgg ccaataaatt
     1621 aagaaactac accatcaact tcggcccgca acaccctgcg gcgcacggcg tattgcgtat
     1681 gattttggag ttggagggcg aaaccatcgt ccgtgccgac ccgcatatcg gccttctgca
     1741 ccgaggcaca gagaaactgg ctgaaaccaa aacctacctg caagccctgc cctatatgga
     1801 ccgtttggac tacgtttcca tgatggtcaa cgagcaggcg tattgcttgg cggtagaaaa
     1861 acttgccggt atcgatgtgc ctatccgcgc ccaatacatc cgcgtgatgt ttgccgaagt
     1921 aacgcgcatc ctcaatcact tgatgggtat cggttcgcac gccttcgaca tcggcgcgat
     1981 gaccgccatc ctttacgcct tccgcgaccg cgaagagctg atggatttgt acgaagccgt
     2041 gtccggcgcg cgtatgcacg ccgcctactt ccgtcctggc ggcgtttacc gcgacctgcc
     2101 cgacttcatg cccaaatatg agagcagcaa attccgcaac gccaaagtat tgaagcagct
     2161 caacgaatcc cgcgaaggca ccatgctcga ctttatcgac gccttctgcg aacgtttccc
     2221 gaaaaacatc gatacactcg aaaccctcct gactgacaac cgtatttgga aacagcgtac
     2281 cgtcggcatc ggcgttgtca ctcctgagcg cgccatgcaa aaaggcttta ccggcgttat
     2341 gttgcgcggt tcaggcgtgg aatgggacgt gcgtaagaca cagccttatg aagtgtacga
     2401 caaaatggat ttcgacatcc ccgtcggcgt caacggcgac tgctacgacc gttacctctg
     2461 ccgtatggaa gaaatgcgcc aatccgtacg catcatcaaa caatgttccg agtggttgcg
     2521 tgtcaatccg ggtccggtca ttaccacaaa ccacaaattc gctccgccca aacgtaccga
     2581 aatgaaaaca ggtatggaag acctgattca ccatttcaaa ctctttaccg agggtatgca
     2641 cgttcccgag ggcgagacct acaccgctgt cgaacatccg aaaggcgagt tcggcgttta
     2701 catcatttca gacggcgcaa acaaacccta ccgcctgaaa atccgcgcac ccggcttcgc
     2761 ccatctgcaa ggcatggacg aaatggcaaa aggccacatg ctcgccgacg tcgttgccat
     2821 catcggtacg caggacatcg tattcgggga ggttgaccga taatgttatc cgcagaatcc
     2881 ttaaaacaaa tcgacatcga gttggcgaaa tatcccgccg accaacgccg ctctgcgatt
     2941 atgggcgcat tgcgtattgc ccagaccgaa aaaggctggc tcgcccccga aaccatcgct
     3001 tttatcgccg actacatcgg catctcgcct gcacaagcct acgaagtcgc taccttctac
     3061 aatatgtacg accttgagcc tgtcggcaaa tacaaactga ccgtttgtac caacctgccc
     3121 tgcgccctac gcggcggtat ggcgaccggc gaatacctca aacaaagact cggtatcggc
     3181 tacggcgaaa ccacgcccga cggcaaattt acccttgtcg aaggcgaatg catgggcgcg
     3241 tgcggcgacg ctccggttat gctggtcaac aaccacagca tgtgcagctt tatgaccgaa
     3301 gaggcgattg agaagaaact ggcggagttg gagtaggtcg tctgaaacga cgatttaaac
     3361 gtaggtcgga tacttgtatc cgacagagtg ggtaaaaagg caaaatgtcg gatttaagaa
     3421 tccgccctac tgaaataccg aaatgccgtc attcccgcgc aggcgggaat ccaccggtaa
     3481 gattcggttt ctgaatttaa taagacattg cttaccattg aggatggatt cccgcctgcg
     3541 cgggaatgac gacagacaag caagtggtcg agatccaaca aaaacgatta aaggtcgtct
     3601 gaaaatatcg atttgataaa ctagatttta tttcagacga cgttacaagc cggtacacaa
     3661 agacatctta aggtcgtctg aaacagcggc cgcaaccgat acgaaaacaa acaggcacac
     3721 caaaaatggc tatttaccaa tcaggcgtga tttttgacca agtggatacc gccaatcccg
     3781 actgctggac attggacgaa tacgtcaaac gcggcggcta taccgccctg cgtaaaattc
     3841 tgtccgaaaa catctcgcaa accgatgtga ttgacgaagt caaaacctcc ggcttgcgcg
     3901 ggcgcggcgg tgcgggcttc ccgactggtt tgaaatggag ctttatgccc cgttccttcc
     3961 cgggcgaaaa atatgtggtt tgtaacaccg acgaaggcga accgggtaca tttaaagacc
     4021 gtgacatcat catgttcaat cctcatgccc tgattgaagg catgattatc gccggttacg
     4081 cgatgggtgc gaaagccggc tacaactata tccacggcga aatcttcgaa ggctaccaac
     4141 gctttgaagc cgctttggag caggcgcgtg ccgcaggctt tttgggtaaa aatattttgg
     4201 gttcggattt tgaatttgaa ctcttcgccc accacggcta cggcgcatat atttgcggcg
     4261 aagaaaccgc attgctcgaa tcgttggaag gcaaaaaagg ccagccgcgc ttcaaaccgc
     4321 cattccctgc ttcgttcggc ctgtacggca aaccgaccac cattaataat actgaaacct
     4381 tttcctccgt cccattcatt atccgtgacg gcggacaggc atttgccgat aaaggtattc
     4441 cgaatgcagg cggtaccaaa ttattctgta tttccggcca tgtcgagcgt ccgggcaact
     4501 atgaagtgcc attgggtacg ccgtttgccg aagtcttgaa aatggcgggc ggtatgcgcg
     4561 gcggtaaaaa actcaaagcc gtcattcccg gcggttcgtc cgcgcccgta ttgcctgccg
     4621 acatcatgat gcagaccaat atggactacg actcgatctc caaagcaggc tccatgctcg
     4681 gttccggcgc gattatcgtc atggacgaag acgtgtgcat ggtcaaagcc cttgagcgtt
     4741 tgagctactt ctactacgac gagtcttgcg gccaatgtac cccctgccga gaaggtacgg
     4801 gctggcttta ccgcatcgtc caccgcatcg tagaaggcaa aggtaaaatg gaagatttgg
     4861 atttgctgga ttccgtcggc aaccaaatgg caggccgcac catctgcgcc ctcgccgatg
     4921 ctgccgtctt ccccgtccgc agctttacca agcatttccg tgatgagttt gtgcattaca
     4981 tcgaacacgg cgggccgatg aaagagcata agtggggagg gtggtaatgg tggaagctaa
     5041 aatttttatt ctatacggtg cagccaacaa aggtaagagt acgacactca atacgctttt
     5101 taatcagatt tgtcggaaat tttctaaatt tctagtcttt tttgaaagac atggaaacgg
     5161 cttagatttt gttgcagtat ttgatcatga aggtcagaga attggttttt attcatctgg
     5221 tgataatgaa tacgaggtta ggggaaattt atacaaactt tattcgcata attgtgattt
     5281 tatttttggc acgtcaagga cacggggtgg tagttgcgat gcagtaggat gttatgcaga
     5341 gttattgcat ggcgatgtaa atataattaa ttggtgtgaa aagtttgagc ctacagatga
     5401 agacaatgag cgtgctgtta aagagttatt taagtcattt aaaaatataa taaatgagtt
     5461 atagttttag ttggttttat attggttaaa agcaaaatgc taaaaattta actttgccgt
     5521 cattcccgcg taggcgggaa tccatagtgg aatttacaga acccgatatt tgaaaagcag
     5581 ttgccgaaat tcaaaaaatg gattcccgcc tacgcgggaa tgacggcggg agtaggcaga
     5641 tgttttcaga tgaaaacggt tgtaaatgat attaaaaaag ttgttgttta tattgcagga
     5701 aaaatgaata cgaaaccatc cgcttactag acaacctgcc gtatatattt tggcaaacgg
     5761 taaaaatgga acactctata tcggtgttac catgaatttg ccggaaaggg tttggcagca
     5821 caaaaaccat gtcaatattg atggctttac tgcccgatat gatgtgcatg atttagtttg
     5881 gtatcagttt tttgagaata tgcctgaagc agttgccaaa gaaaaaacga tgaaaaaatg
     5941 gcgacgtgaa tggaagatta aactgattga agaacaaaat actgaatgat tggacttgtc
     6001 gggcgtgttg tttgtttagt tttatttctg gaactttaaa aactgtcgtt attccagccc
     6061 cacctacgcg cagacaggct acggcgggaa tcaccgcaaa agttaagaaa ccaatgtttg
     6121 aaaacagtta ccgaaaaccc aagaatggat tcacgcctgt gcgggaatga cggcaaggtg
     6181 gcagtaaacg ttttaaacag tattgattgt caatgaaact caaaaggccg tctgaaaccc
     6241 atttttcaga cgacctccat aaaagattat ttatcaaata cccgtaacta ggaacgaacc
     6301 atgttacaaa tcgaaatcga cggcaaacaa gtatctgtgg agcagggcgc gacggtgatt
     6361 gaagccgcgc acaagctcgg tacttatatt ccgcatttct gttaccacaa aaaactttcc
     6421 atcgccgcca actgccgtat gtgtctggtg aacgtagaaa aagccccaaa acccctgcct
     6481 gcctgtgcca cgccggttac agacggcatg attgtgcgta cgcattcggc aaaagcccga
     6541 gaggcgcagg aaggcgtgat ggagttcctg ctcatcaacc atccgcttga ttgtccgacc
     6601 tgcgaccaag gcggcgaatg ccagttgcag gatttggcgg tgggctacgg caaaaccacc
     6661 agccgctaca ccgaagaaaa acgttccgtc gtcggcaaag atatggggtc cttggtttcc
     6721 gccgaggaaa tgagccgctg tatccactgc acccgctgcg ttcgtttcac tgaagaaatc
     6781 gccggtttgc aagaaattgc gatggtgaat cgcggcgaac actccgaaat catgcccttt
     6841 atcggcaaaa cggtggaaac cgagctgtcg ggcaacgtca tcgatttgtg tcctgtcggc
     6901 gcattgacca gcaaaccgtt ccgcttcaac gcgcgtactt gggaattgaa ccgccgcaaa
     6961 tccgtttccg cccatgacgc tttgggcagc aacctgatta tacaaaccaa agaccatacc
     7021 gtccgccgcg tgttgccgct ggaaaacgaa gcgattaacg aatgttggct gtctgaccgc
     7081 gaccgtttcg cctacgaagg cctgtatcac gaaagccgtc tgaaaaaccc gaaaatcaaa
     7141 cagggcggcg agtggatgga cgtggattgg aaaaccgcgt tggaatatgt ccgcagcgcg
     7201 attgaatgta tcgccaaaga cggcaaccaa aaccaagtcg gcatttgggc caatccgatg
     7261 aatacggttg aagagctgta tctggccaaa aagcttgcca atggtttggg tgttaaaaac
     7321 tttgccaccc gtttgcgcca acaagacaaa cgtctttcag acgaccttaa aggtgcacaa
     7381 tggttgggac aaagcattga atctttggct gacaacgatg ccgtattggt agtcggtgcg
     7441 aacttgcgca aagaacagcc gctcctgacc gcccgcctgc gccgcgccgc caaagaccgt
     7501 atggccttga gcgtattggc cggcagcaag gaagagttgt tgatgccgct tctctctcaa
     7561 gaagccgcac atcccgacga gtgggcaggc cgtctgaaaa acctgtctgc caatgcggaa
     7621 cacgccgtta ccgccagcct gaaaaatgct gaaaaagcag cggtgatttt gggcgcggaa
     7681 gtgcaaaacc atcctgatta cgccgccatt tatgctgccg cgcaagagct ggcagccgcg
     7741 accggcgcag tgctgggcat tttgccgcaa gctgccaaca gcgttggtgc ggatgtctta
     7801 aatgtaaact ctggtgagag cgttgccgaa atggcaaatg cgccgaaaca ggcagtcttg
     7861 ctgctcaacg tcgagcctga aatcgatacg gttgacggtg caaaagccgt agccgcattg
     7921 aaacaggcga aaagcgtgat ggcatttacg ccgtttgtca gtgaaacgct gctggacgtg
     7981 tgcgacgtat tgttgccgat tgcgccgttt accgaaacat caggcagctt catcaatatg
     8041 gaaggccgtc tgcaatcttt ccacggcgtg gtacaaggct tcggcgactc gcgtccgatg
     8101 tggaaagtgt tgcgcgtatt gggcaatctg tttgacctga aaggttttga ataccacgat
     8161 accgctgcga ttttgaaaga cgcgctggat gcagaaagcc tgtcgtccaa actggacaac
     8221 cgcagtacat ggacagggga gggcgttcag acggcatcag accgcctcgt ccgtgtcggc
     8281 ggcgtcggta tttatcacac cgatgccatc gtacgccgtt ccgcaccgtt gcaagaaacc
     8341 agccatgccg ccgtgcctgc cgcgcgtgta aatccgaaca cattggcacg cttgggcctg
     8401 caagacggac aaaccgctgt cgccaaacaa aacggcgcaa gcgtatcggt tgctgtcaaa
     8461 gccgatgccg gtttgcctga aaacgtgatg catctgccgc tgcataccga aaatgccgcg
     8521 ctgggtgcgt tgatggacac tattgaactg gcgggagctt gatcatgcaa gaatggttcc
     8581 aaaacctctt tgccgcaacg ctcggtctgg gcgatttggg catcaccgta ggcttggtgg
     8641 tatccgtcat cgtcaaaatc gtgattatcc tgattccgct gattctgacc gtcgcctacc
     8701 tgacttattt cgaacgtaaa gtcatcggct tcatgcagct tcgcgtcggc ccgaacgtaa
     8761 ccggcccgtg gggcctgatc cagccgtttg ccgacgtgtt caaactcttg tttaaagaag
     8821 taacccgtcc gaagctgtca aacaaagccc tgttctacat tggcccgatt atgtcgctcg
     8881 ccccgtcttt tgcggcatgg gcagtgattc cgttcaacga agaatgggtg ctgaccaata
     8941 tcaatatcgg ccttttgtat atcctgatga ttacctcgct gtcggtttac ggcgtgatca
     9001 tcgcgggctg ggcttccaac tccaaatatt cgttcttggg cgcaatgcgt gcttccgcgc
     9061 aaagcatttc ctacgaaatc gccatgagtg ccgcgctggt gtgcgtcgtg atggtgtcgg
     9121 gcagcatgaa cttctccgac atcgttgccg cgcaggcaaa aggcatcgca ggcggttcgg
     9181 tattctcttg gaactggctg ccgctcttcc ccatcttcat cgtctatctg atttccgccg
     9241 ttgccgaaac caaccgcgca ccgtttgacg tggcagaggg cgagtctgaa atcgttgccg
     9301 gtcaccacgt cgaatattcc ggcttcgcat tcgcgctgtt cttccttgcc gaatacattt
     9361 tcatgattct gattgccgcg ctgacatcgt tgatgttcct cggcggctgg ctgtctccct
     9421 tcccgcaaag ctggggcatt gtcggtacgc cttccgcatt ttggatgttc gcgaaaatgg
     9481 cggcggttct gtactggtat ctgtggatcc gtgcaacctt cccgcgctac cgttacgacc
     9541 aaatcatgcg tttgggctgg aaagtgctga ttcccatcgg cttcgcctac atcgtagttt
     9601 tgggtctgtg gatgatttcg ccgctgaatt tgtggaaata agtttcagac ggcatcttga
     9661 ggccgtctga acaaagcgat tttgaatacc taacgaaatc cctgttttga gggaacataa
     9721 atatggctaa cttagtaaaa acctttctgc ttggcgaatt ggtaaaaggt atgggcgtaa
     9781 cgctcaaaaa cttttttgcc cgcaaagaca caatttattt ccccgaagag aaaacgccgc
     9841 aatccgtgcg tttccgcggt ctgcacgcgc agcggcggta tccgaacggc gaggaacgct
     9901 gcatcgcgtg taaattgtgc gaggcagttt gtccggcaat ggcgatcaat atcgaatcgg
     9961 aagaacgcga agacggcacc cgccgcacca aacgttacga catcgacctg accaagtgca
    10021 tcttctgcgg tttctgcgaa gaggcctgcc cgaccgatgc aattgtagaa acccatattt
    10081 ttgaatacca cggcgagaaa aaaggcgact tgcacatgac caagccgatt cttttggcca
    10141 ttggcgataa atacgaagct gaaatagcca aacgcaaagc cgctgacgcg ccgtatcgtt
    10201 aatgctttgg ggcttcttgg aaggttttaa atatggaagg actgattaat gcattgaaat
    10261 atttagccga acatgagcca atagataatt ttgaagaaat tagaactaga aatagtccga
    10321 ttgagttgcc aagtggatta agtaattttg aacaaaatat ttttttaaaa gaaaatttat
    10381 ccccaaaatt acaaaatgat gatagcttga agacgcatta ttggattatc cgtgaatggg
    10441 gtgggattaa aagttttaaa caatctgctg aaaatagcca gcttattcgt caatttttat
    10501 cggaacttaa ttcgggaaaa ttgagtagtg gtttgttgaa aatttcatca ttatctaaat
    10561 tggcttcttt tatagattgt gagcgattcg ccatttatga ttcacgcgct attttttcgt
    10621 tgaattggtt gttgtttaaa tttacaaatg cagatttgtt ttttcagcca caaggtagaa
    10681 atagggaact agaaatccga aatatgaacg tattgtttca tttttctgat atcaaaccga
    10741 attatcggaa accagacgtt tcgtttcatc aatattgtgg attgttacaa gatttggcga
    10801 aacaagttta tggtaaacaa gcaaaaccgt atcacataga aatgttgtta ttcaaaattg
    10861 cgacaacgtg gatttgtgcg gatatggatc aactgattaa gtttgattgt ttgcgtaacc
    10921 aggattttca gactgcttga aaccatattt ttgattaata aagaaagcat agactatgac
    10981 tttccaactg attttatttt atatttttgc agtgataatt ctttatggcg cgctcaaaac
    11041 cgttaccgcc aaaaaccccg tgcatgccgc tttgcatctg gtgctgacct tctgcgtgag
    11101 cgcgatgatt tggatgctga tgcaggcgga gtttttgggc gttacgctgg tggtcgtgta
    11161 tgtcggcgca gtgatggtat tgttcctgtt tgtcgtgatg atgctgaata tcgacatcga
    11221 ggaaatgcgc gccggttttt ggcggcacgc gccggttgcc ggcgtggtcg gtacattgtt
    11281 ggcggttgcc ctgattctga ttttggtcaa cccgaaaacc gaccttgccg catttggtct
    11341 gatgaaagac attcccaccg attacaacaa tatccgcgat ttgggcagcc gtatttatac
    11401 cgaatacctg ttgccgtttg agctggcggc ggtattgctg ctgttgggta tggtggcggc
    11461 gattgcgctg gttcaccgta aaaccaccaa tccgaaacgc atggatcctg ccgaccaagt
    11521 taaagtacgt gccgaccaag gccgtatgcg tctggtgaaa atggaagcgg tcaaaccgca
    11581 agttgaatct gccgaagaga gcgaagtttc agacggcttc aagccggaag gggagggtaa
    11641 agcatgatta ccttgacgca ttatctggta ttgggtgcgc tcctgttcgg tatcagcgca
    11701 atgggtatct ttatgaaccg caaaaacgtg ctggtattgc tgatgtccat cgagctgatg
    11761 cttttggcgg tgaacttcaa ctttatcgcc ttctcgcaac atttgggcga tactgccgga
    11821 caaattttcg tattcttcgt attgaccgtt gccgctgccg aatccgccat cggtttggcg
    11881 attatggtat tggtgtaccg caaccgacaa acgattaatg ttgccgattt ggatgggttg
    11941 aaagggtaaa ggtaggttgg gtcgagacct gacaagacac cgatgccgtc tgaaaacccg
    12001 ataggaaaaa cgatgaaatc catagacgaa caaagcctgc ataatgcccg ccgcctgttt
    12061 gaaagcggcg acatcgaccg tatcgaagtc ggtaccaccg cgggcctgca acagattcac
    12121 cgttacctgt tcggcggctt atatgatttt gcgggtcaaa tcagggaaga caacatttcc
    12181 aaaggcggtt ttcgttttgc caacgccatg tatttaaaag aggctttggt taaaatcgag
    12241 cagatgcccg agcggacttt tgaagaaatc atcgccaaat atgttgaaat gaacattgcc
    12301 catccgtttt tggagggtaa tggcagaagt acccgcatct ggctggattt ggtgctgaaa
    12361 aaaaacctga aaaaagtcgt gaactggcaa aatgtaagta aaaccctgta tttgcaggcg
    12421 atggaacgca gccccgtcaa cgatttagaa ctgcgctttc tgttaaagga caacctgact
    12481 gacgatgtgg acaaccgtga aatcatcttt aaaggtatcg agcagtcgta ttattacgaa
    12541 gggtatgaaa aaggctgagg gtcgtctgaa aagcgatttc agactgtttc agacgacctg
    12601 attcggtagg tgatcagacg ggagcggatg agaaaagaaa ttctgggtaa gaataatccg
    12661 gtctgaaata ttggaagaag aatgatggat aaaaatcagt tagaacaaga atttcataaa
    12721 gccatgttaa atatttatca ggaggctttg aatttgccgc aaccttacaa ggcgacacga
    12781 tttttacaaa ttgtaaatga atttggtggt aaagaggcgg cggataaatt attgagtacg
    12841 ggggaaaaga agactcagac cggttttaca gagctgattc tgagtggtgg cggagtccac
    12901 gccttgaaat acagtatgga atatctggtg ttacaaaagc cgtggtgtga tttatttact
    12961 gaagagcaat tagctgtggc acgcaaacga ttggagcgtg ttggatttgt ttttccgaag
    13021 taattttgta cgaaacaaac atagattttt aaatcaatcg gattcaatca aatgaacgac
    13081 atgactttat atctcatcat tgcccttgtt ccgttggcag gctcgttgat tgcgggtttg
    13141 ttcggcaaca aaatcggtcg tgccggtgcg catactgtta cgatactcgg tgtggcggtg
    13201 tctgccgtgc tgtcggctta tgtgctgtgg ggattcctca atggcagccg tgccaagttt
    13261 gacgaaaacg tctatacctg gctgacaatg ggcggcttgg atttttccgt cggcttcttg
    13321 gttgatacga tgacggcgat gatgatggtc gtggtaacgg gcgtgtcgtt gatggtgcat
    13381 atctacacca tcggctatat gcacgacgaa aaagtcggct accaacgctt cttcagctat
    13441 atttctttgt ttactttcag catgttgatg ctgattgtga gcaacaactt catccagctc
    13501 ttcttcggct gggaggctgt gggcttggtg tcgtatctct tgatcggttt ctatttcaaa
    13561 cgtccaagcg caacatttgc caacctgaaa gcctttttga tcaaccgtgt cggcgacttc
    13621 ggctttttgc tcggtatcgg cttggtgctt gcctatttcg gcggcagcct gcgctatcaa
    13681 gatgtattcg cttatctgcc caacgtgcaa aatgccacca tccagctttt ccccggtgtg
    13741 gaatggtcgt tgattactgt cacctgtttg ctcctgtttg tcggtgcgat gggtaaatcg
    13801 gcacaattcc cactgcacgt ttggctgcct gattcgatgg aaggcccgac cccgatttct
    13861 gcattgattc acgccgcaac catggttacc gccggtttgt ttatggtgtc gcgtatgtcg
    13921 ccgatttatg agatgagcag caccgcgctg tctgtcatca tggtaatcgg tgcgattacc
    13981 gccctgttta tgggtttctt gggcgtgatt caaaacgaca tcaaacgtgt ggttgcgtat
    14041 tccaccctgt cgcaactggg ctatatgacc gtggctctgg gcgcgtctgc ctattccgtg
    14101 gcgatgttcc atgtgatgac ccacgccttc tttaaagcct tgttgttctt ggcggcgggc
    14161 agcgcgatta tcggtatgca ccatgaccaa gatatgcgcc acatgggcaa tctgaaaaaa
    14221 tacatgccga ttacttggct gaccatgctg atcggtaact tgtcgctgat cggtacgccg
    14281 ttcttctccg gcttctattc caaagattcg attatcgaag cggcgaaata cagcaccctg
    14341 ccgggcagcg gctttgccta ttttgccgtc ctcgccagcg tttttgttac tgcgttctac
    14401 gcattccgcc aatactttat ggtgttccac ggcgaagaga aatggcgcag cttgcctgaa
    14461 catcatgacg atcatcatgg cgaagaacat cacagcttgg gcaaaaacga caatccgcac
    14521 gaaagcccgt tggttgttac cctgcctttg attttgcttg ccattccgtc cgtcatcatc
    14581 ggctaccttg ccatcgagcc tatgctttac ggcgatttct tcaaagatgt gattttcgtc
    14641 aacgccgacg cgcatccgac catgcacatc atgaaggaag agttccacgg cgcattggca
    14701 atggtgtcgc acagcctgca ttcgcccgta ctctaccttg ctatcgcagg cgtgttgagc
    14761 gcatggcttt tgtacgtcaa actgccgcac ctgccagcga aaattgcaca gacgttccgt
    14821 ccggtttacg ttttgtttga aaacaaatac tacctcgacg tcctgtattt caacgtcttt
    14881 gccaaaggca cgcgcgcatt gggcactttc ttctggaaag tcggcgatac cgccattatc
    14941 gacaacggca tcgtcaacgg ctctgccaaa ctggtcggcg cgattgccgc gcaagtgcgt
    15001 aaagctcaaa ccggctttat ctatacctac gccgccgcta tggtattcgg cgtattggta
    15061 ctgctcggca tgaccttctg gggattgttc cgataagaat aaggtttcag acggccttaa
    15121 accttcaggc cgtctgaaac gaagaaatat ccacataaac acatttttat tttaaccaca
    15181 ggttaaccac tatgttttcc aactacctac tcagcttggc aatatggata cccatcgccg
    15241 caggcgtgct ggttttggca acggggtcgg acagccgtgc gccgtttgcc cgcgtgctcg
    15301 ccttcatggg tgcgcttgcc ggtttcttgg taacactgcc cctgtttacc ggtttcgacc
    15361 gtttgagcgg cggctatcaa tttaccgagt tccacgagtg gattccgctt ctgaaaatca
    15421 actacgcatt gggcgtggac ggtatttcag tgctctttat catcttgaat gcgtttatta
    15481 cgctgttggt ggtattggca ggttgggaag tcattcagaa acgtccggcg cagtatatgg
    15541 cggcattcct gatcatgtcg ggtttgatta acggcgcgtt tgccgcgcag gatgcgattc
    15601 tgttttatgt gttcttcgag ggtatgctga ttccgctgta cctgattatc ggtgtatggg
    15661 gcggtccgcg ccgcgtctat gcgtcggtca agctcttcct ctacacgctg atgggttcgc
    15721 tcctgatgct ggttgcgatg gtttaccttt attatcaaac aggcagcttc tctattgtcg
    15781 atttccaaaa catcgaacag attccgttgg gcgtacaaca gcttttgttt gtggcgttct
    15841 tcctgtcatt tgccgtaaaa gtgccgatgt tccctgtgca cacttggttg ccggatgccc
    15901 acgttgaagc gccgaccggc ggttcgatgg tgttggcggc cattacgctg aaactgggtg
    15961 cgtatggttt cttgcgcttt atcctgccga ttatgccgga tgcggcacgc tattttgccc
    16021 ccgtgatcat cgtattaagt ctgattgccg tgatttatat cggtatggtg gctttggtgc
    16081 aaaccgatat gaaaaaactg gtggcgtatt cgtccatcag ccatatgggt tttgtaacgc
    16141 ttgggatgtt tttgtttgtt gacgggcagt tggacgactg ggcattgaaa ggtgcaatca
    16201 ttcaaatgat ttcgcacggt ttcgtgtctg ccgcgatgtt tatgtgtatc ggcgtgatgt
    16261 acgaccgcct gcacacgcgc aatattgctg attatggcgg cgtggtcaat gtgatgccca
    16321 agtttgcggc gtttatgatg ctgttcggta tggcgaacgc gggtttgcct gcgacttccg
    16381 gcttcgtggg cgagtttatg gtgattatgg gcgcggtcaa agtgaatttc tgggtcggcg
    16441 cgttggccgc catgaccctg atttacggtg catcttatac cctgtggatg tacaaacgcg
    16501 ttatttttgg tgcgatccac aatccgcacg ttgccgaaat gcaagacatc aattgccgcg
    16561 aatttgcgat tttggcaatt ttggcggtgg ctgttttggg tatgggcctg tatccgaacg
    16621 catttatcga agtggtgcat caggcggcaa acgatttgat tgcccatgtg gcacaaagca
    16681 agatttgagg tgtgtaaatg aactggtctg atttgaattt aatgcccgcc atgcccgaaa
    16741 tcgtgctgct gtcgctgctg gtgttattgt tgctggcgga cttgtgggtc agtgatgaca
    16801 aacgccggtg gacgcattac ggcgcgttgg caacggtggc ggttacggct gtggtgcagt
    16861 tggcggtgtg ggaacagggc agcacgtctt cgttcaacgg gatgtatatt gcagacggta
    16921 tgtcgcgttt ggcaaaaatg gttttatatg ccttgacctt tgccctgttt gtctatgcca
    16981 agccctacaa ccaagtgcgc ggtattttta aaggcgagtt ttacaccctg tcattgtttg
    17041 ccctgttggg tatgagtgtg atggtgagcg cggggcattt tttaactgcc tatatcggtt
    17101 tggaactctt gtcgcttgcc ctttacgccc tgattgccct gcgccgcgat tccggctttg
    17161 ccgccgaagc cgccttgaaa tattttgttt tgggcgcgct ggcatccggc ctgctgctct
    17221 acggtatttc tatggtttac ggcgcaaccg gttcgctgga atttgccggc gtgctcgcct
    17281 cttccttcaa tgaagaagcc aacgaatggc tgttgaaact gggtttggtg tttatcgtcg
    17341 tcgccgtcgc gttcaaactc ggtgcggtgc cgttccatat gtgggtgccc gacgtgtatc
    17401 acggcgcgcc cacttctgtt accgccttgg tcggcactgc cccgaaaatc gccgccgtcg
    17461 ttttcacttt ccgcatcctc gttaccgggc tgggaaccgt gcatcatgac tggtctctga
    17521 tgtttgccct gcttgccgcc gcctcgctgc tggtcggcaa ccttgccgcc atcatgcaga
    17581 ccaatatcaa acgtatgttc gcctattcca ccgtatcgca tatgggtttc atcctgttgg
    17641 cgtttatggc gggcgcggtc ggctttgcgg cgggcctcta ttacgccatt acctacgcgc
    17701 tgatggcggc ggcagggttc ggagtgttga tggtgttgtc ggacggggac aacgagtgcg
    17761 aaaacatcag cgatttggca gggttgaacc aacaccgcgt atggcttgcc tttttgatgc
    17821 tgctggttat gttctctatg gcgggcattc cgccgctgat gggtttttac gccaaattcg
    17881 gcgtgattat ggcactcttg aaacaaggcc atgtttggtt gtctgtattt gccgtcatca
    17941 tgtcgctgat tggtgcgttc tactacctgc gcgtggtcaa agtcatctac ttcgatgtgc
    18001 ctgatcatga ccagccggtc ggcagcaact atgccgccaa atttgttctg acggtcaatg
    18061 ccttcttgct gctcctgtgg ggcatcatgc cgcaaaccgt tatcgactgg tgcgccaagg
    18121 cgttggagaa cacgctgtaa gccgccgcaa cggcagccgt gtcagaggct gccgtttttg
    18181 ttaagatatg ccgttccgca acgcggttca gacggcatcg ccgccgacaa cgcctaaaca
    18241 gaaagcccac catgaccgca tccatgtaca tccttttggt cttggcactc atctttgcca
    18301 acgccccctt cctcacgacc agactgttcg gcgtggccgc actcaagcgc aaacatttcg
    18361 gacaccacat gatcgagctg gcggcaggtt tcgcgctgac cgccgttctt gcctacatcc
    18421 tcgaatcccg tgcaggatcg gtacacgatc agggttggga gttttatgcc acagtcgtct
    18481 gcctgtacct gatttttgcg tttccatgtt ttgtgtggcg gtatttttgg cacacgcgca
    18541 acagggaata gacaagcata ggaatgccgt ctgaaaccct ttcagacggc atttgtttca
    18601 ttcaagtgca ggccggcatc gctgtgccgg cacgtttcag ccggcgatat acgccggttt
    18661 taatatttgc gggcgactgc aaattctgcc aactgccgca ggcgcagggc tttgtcgccg
    18721 aagggttcga gcagcgcgac cgcttcggca accagtttgt gtgcgtatga gcgcgccgct
    18781 tccaagccca tcagtttcac ataagtcggc ttgtcgttgt ctgcgtcttt gcccgccgtt
    18841 ttgcccaaag tcgccgtgtc cgcttcacaa tccaacacat cgtcaatgac ttggaacgcc
    18901 agccccagtt ttgccgcgta agcgtccaat acggaaagtt ccgcatctga cagatcagga
    18961 cacgccgtcg cccccaataa aaccgccgca cggattagcg cacccgtttt caggctgtgc
    19021 atctgttcca aatcggcttg aaccatttgt ttgccgacat tcgccaaatc gattgcctga
    19081 ccgcccgcca tacccctgct gccgcccgct ttcgccaaca ccgacaacat tgccaactgg
    19141 cgtgcggcgg gcagttctgt cggacggctc aacacgtcaa atgcctgtgt ctgcaaagcg
    19201 tcgccggtca gaagggcggt cgcttcgcca tatttgatgt ggcaagtcgg tttgccgcgc
    19261 cgcaggctgt cgttgtccat cgccggcata tcgtcgtgaa ccaaagaata gacgtggatc
    19321 atttcgattg ccgccattgc ctgttctact gcttcatgca cggcttcgcc taattccgaa
    19381 gctgccagaa ccagcatcgg ccgcagacgc ttaccgccgt ccaaagccgc ataacgcatc
    19441 gcttcgtgca gtgtgtgcgg tatttccccc tcagacggta aaaaccgttc aagcagcagc
    19501 tctgtttgcg cctgcgccct ctgttgccac gttttcaaat cattcgtcgg attcaaggtt
    19561 taactccttc agcccgtctg tgtctaaaac ctgtagcttt tgttcgactt gtgccagttt
    19621 ggtttggcag tacctgacca gttcgttgcc ttcctgatag gcggcaagcg cgtcttccaa
    19681 gggcatttcg ccctgcatag actgcgtcag cgattcgagg cgcgacaagg cttcttcaaa
    19741 cgatttcggg gcgtttttct tcatcgtatt tccttttcgg ttgaaacccc gccctttagg
    19801 gcggcaggat cagactttat ttgggagggg tgtaaccctt tccaaatcag ggcaatacat
    19861 agggcggtgc tttatgtgcc gtcctgtgtg ttggaacata gtttcggatg ttccggtaaa
    19921 aagcggattg tagcattttt gaaaaacgga tgccgtctga aacccgaatc cggcttcaga
    19981 cggcattttt tccgcccagg cggcaaggcg ttacccgggc agttcgtcgg tgatgccctg
    20041 caaaaaggcg aggcgttcgg ggcttgccgc cccggtttgc gcggcggctt tgaaggcgca
    20101 gccgggttcg gcgcggtggg tgcagttgtg gaagcggcat tgcccgacaa ggtggcggaa
    20161 atcggggaaa tagcgcggca aatcggcggc ttggaggtgg tgtaaaccaa attcttgcaa
    20221 acccggggag tcgatgagtt gggtttcgcc gttcaaatca taaagccggg cgtgggtggt
    20281 ggtgtgtttt cccgagtcga gtgcggcgga aatgtcgccg gtgcgggcgg tttggctgcc
    20341 caaaagggcg ttggtcaggg tggatttgcc cataccgctc tgcccgagca ggatgttgct
    20401 gtgcccttgc agggcggggc gcaggctgcc ggcgttttcc agtgcgcggg tttcgatgac
    20461 gggataaccc agcgtttcgt agaatttgag tttttcgcgc caaagggcgg tttcgggcag
    20521 gtcggctttg ttcaggacga tgacggcttc aataccggcg gcttcggcgg caagcagggc
    20581 gcgttgcagc agccgcacgc tcggactcgg gacggcggcg gttacgatga ggagttgggt
    20641 aacgttggcg gcgatgagtt tggttttcca cgcgtcttgg cggtagagca ggctttggcg
    20701 cggtaaaaaa tcttcaatca caacttgttc ggcgttgacg gggctgatgc ggacgcggtc
    20761 gccgcaggcg aaatcgacgc gttttttgcg ggtgctggct tcgtaggttg tgccgtcggg
    20821 cgtgcggaca atgtagcggc ggccgtagct ggcggtaatt tgggcggtgt cgttcatggt
    20881 ttctttgggg ttgggtgtgg gaatgccgtc tgaaaacggg tgttcggacg gcatcggttc
    20941 agtcgtgctg ccactcgacg tgttcgttga ggaagccgcc gctctggtgc gcccagagtt
    21001 tggcgtaaag cccgcgtttt tcgaggagtt cggcgtgtgt gccttcttcg atgatgcggc
    21061 ctttgtcgag gacgacgagc ctgtccattg cggcgatggt ggagaggcgg tgggcgatgg
    21121 cgatgacggt tttgccgtcc atcattttgt cgaggctttc ttggatggcg gcttcgactt
    21181 cggaatcgag cgcgctggtg gcttcgtcca aaagaagaat cggtgcgtct ttgagcatca
    21241 cgcgggcgat ggcgatgcgc tggcgttgcc cgccggagag tttcacgccg cgttcgccga
    21301 cgtgtgcgtc gtagccgcgc cgccctttgg catcggaaag gtcggggatg aagccggcgg
    21361 cttcggcgcg ttcggcggca gaaaccattt cggcatcggt cgcgtcgggg cggccgtaaa
    21421 taatgttgtc gcgcacggaa cggtgcagca gcgaggtatc ttgcgtgacc aaaccgattt
    21481 gggcgcgtaa agattcttgg gtaacgccgc ttatgtcctg cccgtcgatc gaaaccgtgc
    21541 cgctttgcgg ttcgtagaag cgcaaaagca ggttgacgat ggtggatttg cccgcgccgc
    21601 tgcgtccgat caagccgact ttttcgcccg ggcggatggt gaggttgaag ccgttgagca
    21661 gcggtttgcc cgcttcgtag gagaaatcga cgtgttcgaa cttgatttcg ccttgcgaca
    21721 ctttcagcgg cagggcgttc ggcttgtcga ggatggtgtg cggtttggac agggttgcca
    21781 tgccgtcgcc gacggtgccg atgttttcaa acagccgcgc ggattcccac ataatgtatt
    21841 gcgacaaacc gttgacgcgc aacgccatcg cggtggcggt ggcgaccgcg cccacgccga
    21901 cctgtccgtt gtgccaaagc cagatgccca gtgcggcggt ggagagggtc agggaggtgt
    21961 tgacgatgaa gctgcacgaa tgcagcagcg tcgccagccg catttgggcg cgcaccgtaa
    22021 ccataaattc ttccatcgac tgcttggcat aggcggcttc acgcgcgccg tgggagaaga
    22081 gtttgacggt ggcgatattg gaataggcat cggtaatgcg gccggtcatc agcgagcggg
    22141 catccgcctg ccatgcggcg gtttgcccca atttgggaat cagcaggcgc atcaccgaag
    22201 cgaaaccgac aatccagccg ataaagggca gcagcagcca tgagtcgagc gaggcgagaa
    22261 tcacgccgga ggtaatgaaa tacaccgaca cataaacgac catatcggca accgtcatca
    22321 ccgcgtcgcg caacgccagc gcggtctgca tgactttggc ggacacgcgt ccggcaaatt
    22381 cgtcctgata aaaaccgagg ctttggttca gcatcaggcg gtggaagttc cagcgcaggc
    22441 gcatggggaa cacgccctga agggtttgca ggcgcacgtt ggacgcggca aacgcccaca
    22501 cgaccgaaaa taccatcatc gccgccattg ccgccagttc ccaacctttt tcggcaaaca
    22561 gttcggcggg cgcgtatttg ccgagccact ccacgatttt gcccataaat tgaaaaatca
    22621 gggcttccat aataccgatg ccggcggtca gcgcagccag ggcggctatc catttccgca
    22681 cgccggccat gctgctccag acaaaccgcc acaagccttt ttctggcgtt ttcggggcgg
    22741 cttcgggata agggtcgatt cgggactcga accaggaaaa tattttgttc aacattgttt
    22801 tcgatttcgg taaaacagtt tcagacggca tcaaacacaa tgccgtctga aaggaaggac
    22861 aataacgcca ttttacggga aaagccgtcg ggaagacagc gcgaggcgga aacgcagggt
    22921 ttcgtcaggg caaacgccgc gccgccttca gacggcatta tttcagcagg tttttcaaag
    22981 caaggcgcac gccttcgccc acgtccgtcc cctccggaac gcctttgacc gccgcttttg
    23041 cttcgcgttc gctgtaaccc agcgcaagca gcgtgctgac gatgtcttcc gtttcgtcgg
    23101 cggcgggtgc ggcggcaaac agcccgtccg ttaccgcatg cgcgaccagc ttgccgcgca
    23161 gttccaagac catacgttcg gcggtttttt tgccgattcc cggcgcggaa gacaggcgtt
    23221 tgacatcttc ttcggcaacc gcccgcgcca gctcgtctgc cgtcatcgct gacaaaatgc
    23281 ccaaagccgt tttcgcgccg atgccgccga ccttaatcag ttggcggaag gtcttgcgtt
    23341 cttccgcagt ggcaaaacca aataaaagat gtgcgtcttc ccgaatgata agctgggtaa
    23401 acagttgtac gctttcaccc acgggcggca ggttgtagaa ggtctgcatc gatacgtcgg
    23461 cctcatagcc gacaccgttg acatcgatga cgatttgcgg agggtttttt tcaaccagtt
    23521 tgccggtcag tctgctgatc atgtgtgccg aatcctgaag tgtcgggtgc aaaatgccgt
    23581 ctgaaaccgg ttcgggcttc agacggcacg gattgtatca aattcagtcg tcgcggcggg
    23641 aggaaatcac gcggccggta cgggcatcga caacgacttt gtattcctgt ccgtttttga
    23701 cgatttcgac atcatagtgc ggacggccgt tgtcgtgttc gagatcgatg tcggtgattt
    23761 tgccgccgac acgcgccaac gctgcttttt cggcttgggc gcggctgatg attttgtctt
    23821 gtttgttgtg ttggtgtgcg gcgtgtccgt ggtcgtcatc gccgtgtccg tcgtggtggg
    23881 cgagcgcggg ggcggaaatg ctcagcagtg cggttgcggc ggaggtcaag agaaggtgtt
    23941 tgatgttcat attttgcctt tgtaaatcgt gggttggaaa atgtggatat taataaggta
    24001 tcaaataacc gtcagccggc ggtcaatacc gcccgaacca taccgcgcgc ctgagcttcg
    24061 gcttcggcgg cgcgttcctg cgaggtaaac ggtcccattt tgacgacgta ttcgtaacgg
    24121 cgtttttcaa ccgagaggtt cgtacccgat gacgaaacgg cgaagttttg ggcggcttgg
    24181 ttcagatagg cttgtgcttc gtgttccgta ccgaaagatt tcaagtcgat aaagatgtct
    24241 ttgttttcgg caaccggtgc ggattggccc gggacgattt gttcgatttt gacgtgtgcc
    24301 gtcccttggt tgacaaagcc caatttttgc gcggcggctt tggatacgtc gatgatgcgg
    24361 ttgccgtgga aggggccgcg gtcgttgacg cggacgatga cgcttttgcc gtttttggta
    24421 ttggttacgc gcacatagct ggggatgggc agggttttgt gggcggcggt aaaggcgttc
    24481 atatcgtatc gttctccgcc ggaagttttg cgcccgtgaa acctgccgcc gtaccacgag
    24541 gcgttgccgg tttgcgtgaa ttcggcgact tggtttttcg gcgtgtagcg ttttccggcg
    24601 actttgtagc tgcggttggc ggaggcgtgc agtttttctg ccttgaccac tgcgtcggcg
    24661 gatgccgtct gaagggagtg tgtgccgaat gcggcggtga gaaggaaaag ggtttttcgg
    24721 gttaaagtca aaacgtgttc cgttcttgag ttgaagacga atgggcatca tgcccgccgg
    24781 atacgttccg aaccgccgta cagtgcggac ggcggttcgg aatgtgtccg gataggtttt
    24841 cagacggcat gaacctgcgt tcaaacgccg cctgcgtaac cgtgttgccg ccacgcttca
    24901 aagagaatca cggcgacggt gttggaaagg ttcatactcc ggctgccggg ctgcatcggc
    24961 aggcggattt tttgcgcggc gggcaggctg tcgaggatgt cggcaggcag tccgcgcgtt
    25021 tccggcccga acagtaaaac gtcgcctttt tgaaacgcgg tttcatcggg gcgcgccgtg
    25081 cctttggtgg tcagggcgaa aatgcgcctg cctgcgagtg ccttgaggca gtcgtcgaag
    25141 ttttcgtgca ccgtcaggct ggcgaactcg tggtagtcga gcccggcgcg tttcattttg
    25201 gcggaatcca atgggaagcc gagcggtttg acaaggtgca aatccgcgcc ggtattggcg
    25261 cacaggcgga tgatgttgcc cgtgttcggc gggatttccg gctggtataa aacgatggta
    25321 aacataaata tcaatcactt ataggcgcgt aaccttgcca caaggcggat ggggtgtcaa
    25381 aaaatttagt tattttttca ttggcgtgcg tgccagcgtc cagcagcaga ttcggtttgc
    25441 gcccgatttt ttcagcgtct ttgccaattc gtccagcgtc gcgccggtgg taaagacatc
    25501 gtcgattaac aaaatattac agtttttcgg cggcggtgtg cggatttcaa aggcgttttt
    25561 gatatttcgc cgccgttcgc cgcctttgag cgtgctttgc ggcggacggt ggtgtcggaa
    25621 tacggtgtgt cggggcagta tccgccagcc gtagcgttgc gccaacaacc cgacgatgcc
    25681 ctcgctttgg ttgaacccgc gttgcagcag ccgctccctg cttagcggaa cgggcaggac
    25741 gaaatcgaaa cattcgtctg caagcaggtc gggcggattc tgcatcatca ggtctgccag
    25801 cggctgcacc atgcccaaat cagccaagtg cttcagcgcg cgtatcatat tgctgacggg
    25861 cggttcgtaa tgcagcgatg cccacatccg gtcgaatgcg ggcggttttt tctgacagcc
    25921 gccgcatacc gcgccgcctt gaacgtgcct gaaacacagg gggcagctgt ttgccgcgtc
    25981 ggtgcggtat gccgccaaat cgttgcggca gccggcgcag atgccgtctg aaacgccgga
    26041 cgaaccgtgg cataatacgc aacgcctgat agtgggcgcg tctgcgatgc gccgccaaca
    26101 agagagaaaa tccatgcctg atgccgtcaa aaaagtttac ctgatacacg gttggggggc
    26161 gaaccgccac gtgttcgacg atttgatgcc gcgcctgcct gcaacgtggc cggtgtccgc
    26221 cgtcgatttg cccggacacg gcgacgcgcc cttcgcgcaa cccttcgata ttgaggcagc
    26281 cgccgacgct gttgccgcac aaatcgatac gcccgccgac attctcggct ggtcgctcgg
    26341 cggattggtc gcgctgtatc tggcggcgcg ccatcccgac aaagtccgtt cgctctgcct
    26401 gacggcgagt ttcgcacggc tgacggctga cgaagactat cccgaagggc ttgccgcgcc
    26461 tgcattgggc aaaatggtcg gcgcgttccg aacggattat gccaaacata tcaaacagtt
    26521 tctacaactc cagcttctgc acacgcctga tgcggacgga atcataggca gaatcctgcc
    26581 cgatttggcg cgctgcggca cgccctccgc cttacaggaa gctttggacg cggcggaaag
    26641 ggcggatgcg cggcatttgt tggacaagat agatgttccg gtactgctgg tgttcggtag
    26701 caaagacgcg attacgccgc cgcgcatggg cgagtatctg caccgccgtt tgaaaggcag
    26761 caggctggtt gtgatggaaa aggcggcgca tgcgccgttt ttgagccatg cggaagcgtt
    26821 tgccgcgctg taccgcgact ttgtcgaagg ggtttgacat gaatccgcag gacaaatgct
    26881 ggcaggttca ccgccatctc gccgaatatg ccgaccaacg cctgacactc gtccgcaacg
    26941 cgcccaagta tatcctgctt gcaggtgcgg atgcggacat cagccgcagc ctgctggcga
    27001 aacgctatcc gcaggcggta tttgaagaat acgattcccg tgcggatttt ttggcggctg
    27061 ccgctgccgc ccgcaaaggc ggtttttggc aaaggtttac gggtaagggc gtggtgcaac
    27121 actgccaatc cccgatcgcg ccgctgcccg aagcgtgtgc cgatatgttg tggtcgaatc
    27181 tcggactgtt ggcggcggaa caaatccttc ctgtgctgca caactgggcg cgcgccttga
    27241 agacggacgg gctgctgttt tttacctgct tcgggcgcga cacttttacc gaactgaaat
    27301 gccgtctgaa agaaaacggc attgaaagcc gcagcgtgat gtttcccgat atgcacgact
    27361 tgggcgatat gcttgctgaa aacggctttt acgaccccgt taccgatacg gcgaagctgg
    27421 tgttggatta caaaaaggcg gaaacgtttt gggcggatat ggacacgctg ggcgtttggc
    27481 gggcgatggc gtggaacgat gaaaacgccg cgcgttcgtg tgtcgggaca atatttgagc
    27541 gggaaggcgg tttgggcatt acgctggaaa cggtgtacgg acacgccgtg aaaaaactga
    27601 tgctgccgca aggggagaac gtggtgcagt tttttccgaa gagatgatgt gcagatgccg
    27661 tctgaagccg tttccaagtt tcagacggca tttgtttgtg aaaaccgaca gaactaaagg
    27721 aaatgccgat gtatagtg
//
LOCUS       DAXHNE010000011        27332 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.011, whole genome shotgun sequence.
ACCESSION   DAXHNE010000011 DAXHNE010000000
VERSION     DAXHNE010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27332
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.011"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            25..1107
                     /gene="mraY"
                     /locus_tag="ACFM4Z_000356"
     CDS             25..1107
                     /gene="mraY"
                     /locus_tag="ACFM4Z_000356"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFC6020457.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            1222..1440
                     /locus_tag="ACFM4Z_000357"
     CDS             1222..1440
                     /locus_tag="ACFM4Z_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFC6020458.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     YNLGAMYYKGHGVRQDRALAQEWLGKAC"
     gene            1578..2915
                     /gene="murD"
                     /locus_tag="ACFM4Z_000358"
     CDS             1578..2915
                     /gene="murD"
                     /locus_tag="ACFM4Z_000358"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFC6020459.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRDDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIVTQDIPLQGLHN
                     AANVMAAVALCEAIGLSREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALVGKAKGVFLIGVDAPQIRRDLDGCGLN
                     MTDCATLGEAVQTAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            3041..4366
                     /locus_tag="ACFM4Z_000359"
     CDS             3041..4366
                     /locus_tag="ACFM4Z_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFC6020460.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGLLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            4370..5437
                     /gene="murG"
                     /locus_tag="ACFM4Z_000360"
     CDS             4370..5437
                     /gene="murG"
                     /locus_tag="ACFM4Z_000360"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFC6020461.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPVTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGGLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            5592..7001
                     /gene="murC"
                     /locus_tag="ACFM4Z_000361"
     CDS             5592..7001
                     /gene="murC"
                     /locus_tag="ACFM4Z_000361"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFC6020462.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYLEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLALSKQI"
     gene            7114..8028
                     /locus_tag="ACFM4Z_000362"
     CDS             7114..8028
                     /locus_tag="ACFM4Z_000362"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFC6020463.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            8018..8746
                     /locus_tag="ACFM4Z_000363"
     CDS             8018..8746
                     /locus_tag="ACFM4Z_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFC6020464.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKTLGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            8832..10076
                     /gene="ftsA"
                     /locus_tag="ACFM4Z_000364"
     CDS             8832..10076
                     /gene="ftsA"
                     /locus_tag="ACFM4Z_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFC6020465.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            10195..11373
                     /gene="ftsZ"
                     /locus_tag="ACFM4Z_000365"
     CDS             10195..11373
                     /gene="ftsZ"
                     /locus_tag="ACFM4Z_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFC6020466.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            11512..12822
                     /locus_tag="ACFM4Z_000366"
     CDS             11512..12822
                     /locus_tag="ACFM4Z_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFC6020467.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            13448..14323
                     /gene="prpB"
                     /locus_tag="ACFM4Z_000367"
     CDS             13448..14323
                     /gene="prpB"
                     /locus_tag="ACFM4Z_000367"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFC6020468.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            14409..15563
                     /gene="prpC"
                     /locus_tag="ACFM4Z_000368"
     CDS             14409..15563
                     /gene="prpC"
                     /locus_tag="ACFM4Z_000368"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFC6020469.1"
                     /translation="MTETTQTPTLKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            15716..16504
                     /locus_tag="ACFM4Z_000369"
     CDS             15716..16504
                     /locus_tag="ACFM4Z_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFC6020470.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFVGGVAGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIAATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIVSMF"
     gene            16582..19188
                     /gene="acnD"
                     /locus_tag="ACFM4Z_000370"
     CDS             16582..19188
                     /gene="acnD"
                     /locus_tag="ACFM4Z_000370"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HFC6020471.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNRQIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDALGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELNGKRQAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAAKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRFGLKRKPWVKSSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRVHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVRNEDGSVRQGSLARVEPEGKTMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPGTNRH
                     TLQLDGTETYDVIGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            19541..20710
                     /gene="prpF"
                     /locus_tag="ACFM4Z_000371"
     CDS             19541..20710
                     /gene="prpF"
                     /locus_tag="ACFM4Z_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HFC6020472.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFAIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            21124..22323
                     /locus_tag="ACFM4Z_000372"
     CDS             21124..22323
                     /locus_tag="ACFM4Z_000372"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC6020473.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            22431..23051
                     /locus_tag="ACFM4Z_000373"
     CDS             22431..23051
                     /locus_tag="ACFM4Z_000373"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC6020474.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNQGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKEVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRRGCGDTAVFGL"
     gene            complement(23178..23621)
                     /locus_tag="ACFM4Z_000374"
     CDS             complement(23178..23621)
                     /locus_tag="ACFM4Z_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFC6020475.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLTTPPDRINIGSVVRHLEPMQLVECMGSNNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            23795..24163
                     /locus_tag="ACFM4Z_000375"
     CDS             23795..24163
                     /locus_tag="ACFM4Z_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC6020476.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEETKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            24208..25362
                     /locus_tag="ACFM4Z_000376"
     CDS             24208..25362
                     /locus_tag="ACFM4Z_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC6020477.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVVAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(25419..26291)
                     /locus_tag="ACFM4Z_000377"
     CDS             complement(25419..26291)
                     /locus_tag="ACFM4Z_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFC6020478.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHHCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALIENLWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            26376..27188
                     /locus_tag="ACFM4Z_000378"
     CDS             26376..27188
                     /locus_tag="ACFM4Z_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFC6020479.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSSEAGKVMNTLL
                     VYGRDGVRTGLYHKMHLFGFSGLGERYAEADTISAGRAVPHLSAEGVPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRTRAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKYRVLDAV"
BASE COUNT         6191 a         7072 c         7700 g         6369 t
ORIGIN      
        1 gacaaaacaa aaaaaggaag ccccatgttt ttatggctcg cacatttcag caactggtta
       61 accggtctga atatttttca atacaccaca ttccgcgccg tcatggcggc gttgaccgcc
      121 ttagcgtttt ccctgatgtt cggcccgtgg acgatacgca ggctgaccgc gctcaaatgc
      181 gggcaggcag tgcgtaccga cggcccgcaa acccacctcg tcaaaaacgg cacgccgacg
      241 atgggcggtt cgctgattct gaccgccatt accgtgtcca ccctgttgtg gggcaactgg
      301 gcgaacccgt atatctggat tctcttgggc gtactgcttg ccaccggtgc gctcggtttt
      361 tacgacgact ggcgcaaagt cgtctataaa gaccccaacg gcgtgtccgc caaattcaaa
      421 atggtgtggc agtcaagcgt tgccattatc gccggtttgg cattgtttta ccttgccgcc
      481 aattccgcca acaatatttt gattgtcccg ttcttcaaac aaatcgccct gccgctgggc
      541 gtggtcggct ttttggtgtt gtcttacctg accatcgtcg gcacatccaa tgccgtcaac
      601 ctcaccgacg gcttggacgg ccttgcgacc ttccccgtcg tcctcgttgc cgccggcctc
      661 gccatcttcg cctatgccag cggccactca caatttgccc aatacctgca attaccttac
      721 gttgccggcg caaacgaagt ggtgattttc tgtaccgcca tgtgcggcgc gtgcctcggt
      781 ttcttgtggt ttaacgccta tcccgcgcaa gtctttatgg gcgatgtcgg tgcattggca
      841 ttgggtgccg cgctcggtac cgtcgccgtc atcgtccgcc aagagtttgt cctcgtcatt
      901 atgggcggat tatttgtcgt agaagccgta tccgttatgc ttcaagtcgg ctggtacaag
      961 aaaaccaaaa aacgcatctt cctgatggcg cccatccacc accattacga acaaaaaggc
     1021 tggaaagaaa cccaagtcgt cgtccgcttt tggattatca ctatcgtctt ggtgttgatc
     1081 ggtttgagta ccctcaaaat ccgctgaacc tatgccgtct gaacatcttt cagacggcat
     1141 ttgaacgcgc aataaacctg cggcgacaat ccgcccagcc ttatcgttaa cggcggctta
     1201 tactaaaaca gaagtaaaac catgaaacag acagtcaaat ggcttgccgc cgccctgatt
     1261 gccttgggct tgaaccaagc ggtgtgggcg gatgacgtat cggattttcg ggaaaacttg
     1321 caggcggcag cacagggaaa tgcagcagcc caatacaatt tgggcgcaat gtattacaaa
     1381 gggcacggcg tgcgccaaga ccgcgccctt gcacaagaat ggcttggcaa ggcttgttaa
     1441 aacggaaacc aagacggctg cgacaatgac caacgcctga aggcgggtta ttgaacagct
     1501 cgcgatgccg tctgaaagcg gcttgggcag gggcggacat ctcctgctca atatgatttg
     1561 ttttaggaca aaccaaaatg acttttcaaa acaaaaaaat cctcgtcgcc ggactcggcg
     1621 gtacgggtat ttccatgatt gcctatctgc gcaaaaacgg cgcggaggtt gccgcgtatg
     1681 atgcggagct gaaaccggag cgcgtgtcgc aaatcggtaa gatgtttgac gggttggtgt
     1741 tttacacggg ccgtctgaaa gatgcgctgg acaacggttt cgatattctg gctctcagtc
     1801 ccggcatcag cgagcggcag ccggatattg aggcgttcaa gcaaaacggc ggacgcgtgt
     1861 tgggcgacat cgaattgctg gcggacattg tgaaccgccg ggacgacaag gtaattgcga
     1921 ttaccggcag caacggcaaa accacggtaa cgagcctggt cggctatctc tgtatcaagt
     1981 gcgggctgga taccgttatc gcgggcaata tcggcacgcc ggttttggag gcggaatggc
     2041 agcgcgaagg caaaaaggcg gacgtgtggg tgttggagct ttccagcttc caactggaaa
     2101 acaccgaaag cctgcgtccg actgcggcga cggtgctgaa catttccgaa gaccatctcg
     2161 accgctacga cgacttgctc gactatgcgc ataccaaagc caagattttc cgtggcgacg
     2221 gcgtgcaggt tttgaatgcg gacgatgcgt tctgccgcgc gatgaagcgt gccgggcgcg
     2281 aggtaaaatg gttttcgttg gaacacgaag ctgatttctg gttggaacgc gagacaggcc
     2341 gcctgaaaca aggcaatgaa gatttgattg tcacgcaaga cattccgttg caaggtctgc
     2401 acaacgccgc taacgtcatg gctgccgtgg ctttgtgtga ggccatcggt ttgtcgcgcg
     2461 aagcattgct cgaacacgtc aaaaccttcc aaggcctgcc gcaccgcgtg gaaaaaatcg
     2521 gcgagaaaaa cggcgtggtg tttatcgacg acagcaaagg cacgaatgtc ggcgcgactg
     2581 ccgccgcgat tgccggtttg caaaatccgc tcttcgtgat tttgggcggc atgggtaaag
     2641 ggcaggactt cacgcccctg cgcgatgcac tggtaggcaa ggcaaaaggc gtgttcttga
     2701 ttggtgtcga tgcgccgcaa atccgccgcg atttggacgg ctgcggcttg aatatgaccg
     2761 actgcgccac tttgggagaa gccgttcaga cggcatatgc ccaagccgaa gcaggcgata
     2821 ttgtgttgct cagccccgcc tgcgcgagct ttgatatgtt caaaggctac gcgcaccgtt
     2881 cggaagtgtt tatcgaagcg tttaaggctt tgtgatgccg tctgaaatgc aaacgccgtc
     2941 attgttgggc ggcaagtaaa gatttagaat accgatttgg gatgtatcgt atgttcggac
     3001 ggcattgtct gccgtctgaa atttttgccc tttgcggcag gtgcaaacag actggcaggt
     3061 ggtttttttg aagatttcgg aagtattggt aaaagtgggc gacggtgtcc acactctgct
     3121 gctcgacagg ccgattgtgc gcgacggcag gaaattcgac gcgccgcttt tgtggatggt
     3181 ggtgctgatg acggcgttca gcctgctgat gatttattcg gcttctgtgt atttggcatc
     3241 aaaagaaggc ggcgatcagt ttttctattt gaccagacag gcggggttcg tcgttgccgg
     3301 cttgatagcg agcggtttgt tatggtttct ttgcaggatg aggacatggc ggcggcttgt
     3361 gccgtggatt tttgccctat ccggcctgtt gctggtagtc gtattgattg ccgggcgcga
     3421 aatcaatggc gcgacccgtt ggataccttt gggtccgttg aatttccagc cgaccgagct
     3481 gttcaagctg gcggtcatcc tttatttggc aagcctgttc acgcgccgtg aagaagtgtt
     3541 gcgcagcatg gaaagtttgg gttggcagtc gatttggcgg gggacggcca atctgatcat
     3601 gtccgccacc aatccgcagg cacgtcgtga aacattagaa atgtacggcc gtttccgggc
     3661 gatcatcctg ccgattatgc tggtggcgtt cggtttggtg ctgataatgg tacagccgga
     3721 tttcggttcg tttgtcgtca ttaccgtcat tgccgttgga atgctgtttt tggcaggatt
     3781 gccgtggaaa tattttttcg tcctggtagg cagcgtcttg ggcgggatgg tgctgatgat
     3841 taccgccgct ccctaccgtg tgcagcgggt agtggcattt ttggacccgt ggaaagaccc
     3901 gcagggtgcc ggctaccagc ttacccactc tctgatggca atcgggcgcg gagagtggtt
     3961 cggtatgggt ttgggtgcga gtttgagcaa acgcggcttt ctgccggaag cgcataccga
     4021 ttttattttt gccatcatcg ccgaagaatt cggtttcttc ggtatgtgcg tgctgatatt
     4081 ctgttacggc tggctggtgg tgcgggcgtt ttccatcggc aagcagtcgc gcgatttggg
     4141 tttgactttc aacgcctata tcgcttcggg tatcggcatt tggatcggta tccaaagttt
     4201 cttcaatatc ggtgtgaaca tcggtgcttt gccgaccaaa ggtctgacgc tgccgttgat
     4261 gtcctatggc ggttcgtcag tctttttcat gctgatcagc atgatgctgc tgttgcgtat
     4321 agattatgaa aaccgccgga aaatgcgcgg ttatcgggtg gagtaaatca tgggcggtaa
     4381 aacctttatg ctgatggcgg gcggaacggg cggacatatt ttccccgcgc tggcggtggc
     4441 ggattcattg cgcgcgcgcg gccatcatgt gatttggctg ggcagcaagg attcgatgga
     4501 agagcgtatc gtgccgcaat acggcatacg cttggaaacg ctggcgatta aaggcgtgcg
     4561 cggcaacggc atcaaacgca aactgatgct gccggttact ttgtatcaaa ccgtccgcga
     4621 agcgcagcgg attatccgca aacaccgtgt cgagtgcgtc atcggcttcg gcggcttcgt
     4681 taccttcccc ggcggtttgg cggcgaagct attaggcgtg ccgattgtga ttcacgagca
     4741 aaacgccgtg gcaggtttgt ccaaccgcca cctgtcgcgc tgggcgaagc gggtgttgta
     4801 cgcttttccg aaagcgttca gccacgaagg cggcttggtc ggcaaccccg tccgcgccga
     4861 tattagcaac ctgcccgtgc ctgccgaacg cttccaaggg cgtgaaggcc gtctgaaaat
     4921 tttggtggtc ggcggcagtt tgggcgcgga cgttttgaac aaaaccgtac cgcaggcatt
     4981 ggctttgctg cccgacaatg cgcgtccgca gatgtaccac caatcgggac ggggcaagct
     5041 gggcagcttg caggcggatt acgacgcgct gggcgtgaaa gccgaatgcg tggaatttat
     5101 taccgacatg gtgtccgcct accgcgatgc cgatttggtg atttgccgtg ccggcgcgct
     5161 gacgattgcc gagttgacgg cggcgggatt gggtgcgttg ttagtgccgt atcctcacgc
     5221 ggttgacgat caccaaaccg ccaacgcgcg ttttatggtg caggcggagg cgggattgct
     5281 gttgccgcaa acccagttga cggcggaaaa actcgccgag attctcggcg gcttaaaccg
     5341 cgaaaaatgc ctcaaatggg cagaaaacgc ccgtacgttg gcactgccgc acagtgcgga
     5401 cgacgtggcg gaagccgcga ttgcgtgtgc ggcgtaaact gccgaaccat gccgtctgaa
     5461 aagccattca gacggcatgg atgtttttta tttcaatccg ctatatattt gtcagaaaac
     5521 tatggcgcgc aaacggtcag ccctttaaaa taacgccttt acgcatcgaa aatccaccgg
     5581 aacgcaacat tatgatgaaa aatcgagtta ccaacatcca ttttgtcggt atcggcggcg
     5641 tcggcatgag cggcatcgcc gaagtcttgc acaatttggg ctttaaagtt tccggttcgg
     5701 atcaggcgcg aaatgccgct accgagcatt tgggcagcct gggcattcaa gtttatcccg
     5761 gccataccgc cgaacacgtt aacggtgcgg atgtcgtcgt tacctctacc gccgtcaaaa
     5821 aagaaaatcc cgaagttgtc gctgcgttgg agcagcaaat tcccgttatt ccgcgcgccc
     5881 tgatgttggc ggagttgatg cgcttccgtg acggcatcgc cattgccggc acgcacggca
     5941 aaaccacgac caccagcctg accgcctcca tcctcggcgc ggcaggactt gacccgactt
     6001 tcgttatcgg cggcaaactc aacgccgcag gcaccaacgc ccgcttgggc aaaggcgaat
     6061 acatcgttgc cgaagccgac gagtcggatg catcctttct gcacctgaca ccgattatgt
     6121 ccgtcgttac caatatcgac gaagaccata tggataccta cgggcacagc gtcgaaaaac
     6181 tgcatcaggc gtttatcgat ttcatccacc gtatgccctt ctacggcaaa gcctttttgt
     6241 gtattgacag cgaacacgtc cgcgcgattt tgcccaaagt gagcaaacct tatgctactt
     6301 acggtttgga cgataccgcc gacatctacg ccaccgacat cgaaaacgtc ggcgcgcaaa
     6361 tgaaattcac cgtccatgtt caaatgaaag gacatgagca ggggtcgttt gaagtcgtgc
     6421 tgaatatgcc cggcagacac aacgtgctga acgcattggc agccatcggc gtggcgctgg
     6481 aagtcggcgc atcggttgaa gcgatccaaa aaggcttgct cggctttgaa ggcgtcggcc
     6541 gccgcttcca aaaatacggc gacatcaagt tgccaaacgg cgggaccgcg ctcttggtgg
     6601 acgactacgg acaccacccc gtcgaaatgg cggcgaccct tgccgccgca cgcggcgcgt
     6661 atctggaaaa acgtttggta ctcgccttcc agccgcaccg ctatacccgc acgcgcgatt
     6721 tgtttgaaga ctttaccaaa gtcctcaata ccgttgacgc gctggtgctg accgaagttt
     6781 atgccgccgg tgaagagccg attgccgccg ccgattcccg cgctcttgcc cgcgccatcc
     6841 gcgtgttggg caaactcgag ccgatttact gcgaaaacgt tgccgatctg cccgaaatgc
     6901 tgttgaacgt tttgcaggac ggcgacatcg tgttgaatat gggcgcggga agcatcaacc
     6961 gcgtccccgc cgcgctgctg gcattgtcga aacagatttg aggcacaccc gcctgacaga
     7021 cggaacatca tataaagatc gtctgaaacc gcaaatcagg tttcagacga cctctggcaa
     7081 caagcataaa gcaatcagga aagaacaaaa acaatgcaga attttggcaa agtggccgta
     7141 ttgatgggcg gtttttccag cgaacgagaa atctcgctgg acagcggcac cgccattttg
     7201 aatgctttaa aaagcaaagg catagacgca tacgccttcg atcctaaaga aaccccattg
     7261 tctgaattga aggcacaagg ttttcagacg gcattcaaca tccttcacgg tacttacggc
     7321 gaagacgggg cggttcaggg tgcattggaa ctgttgggca ttccctatac cggcagcggt
     7381 gtcgccgcat ccgccatcgg catggacaaa taccgctgca aactgatttg gcaggcattg
     7441 ggattgcccg ttcccgagtt cgccgtcctg cacgacgaca ctgatttcga tgccgtcgaa
     7501 gaaaaattgg gcctgccgat gtttgtgaaa ccggcggccg aaggcagcag cgtaggcgtg
     7561 gtaaaagtca aaggaaaagg ccgtctgaaa agcgtttacg aagaattgaa acaccttcag
     7621 ggcgaaatca ttgccgaacg ttttatcggc ggcggcgaat attcctgccc cgtcctgaac
     7681 ggcaaagggc tgcccggcat acacatcatt cccgcaaccg agttttacga ctacgaagcc
     7741 aagtacaacc gcgacgacac catttatcaa tgtccttcgg aagatttgac cgaagccgaa
     7801 gaaagcctga tgcgcgaact ggcggttcgc ggcgcgcagg caatcggtgc ggaaggctgc
     7861 gtgcgcgtcg atttcctcaa agataccgac ggcaaactct atctgttgga aatcaacacc
     7921 ctgcccggta tgacgagcca tagtttagta ccgaaatccg ctgccgttac gggcgtgggt
     7981 tttgccgatt tatgtattga aattttgaag accgcacatg tgggataatg ccgaagcgat
     8041 ggaacggctg acgcgctggc tgcttgtcat gatggcgatg ctgcttgctg cgtccgggct
     8101 ggtttggttt tacaattcga atcatctgcc cgtcaagcag gtgtcgctga agggcaacct
     8161 ggtttattcc gataagaaga cattgggcag tttggcgaaa gaatacatcc atgggaatat
     8221 tttgaggacg gacatcaatg gcgcacagga ggcctaccgc cggtatccgt ggattgcgtc
     8281 ggtcatggtg cgccgccgtt ttcccgacac ggttgaggtc gtcctgaccg agcgcaagcc
     8341 ggtcgcgcgt tggggcgacc atgccttggt ggacggcgaa ggcaatgttt ttgaagcccg
     8401 cttggacaga cccggaatgc cggtattcag aggcgcggaa ggaacgtctg ccgaaatgct
     8461 ccgccgttat gacgaatttt cgactgtttt ggcaaaacag ggtttgggca tcaaagagat
     8521 gacctatacg gcacgttcgg cgtggattgt cgttttggac aacggcatca ccgtcaggct
     8581 cggacgggaa aacgagatga aacgcctccg gctttttacc gaagcgtggc agcatctgtt
     8641 gcgtaaaaat aaaaatcggt tatcctatgt ggatatgagg tataaggacg gattttcagt
     8701 ccgctatgct tccgacggtt tacccgaaaa agaatccgaa gaatagtggg aacaggtatc
     8761 ggacagatta cggccgtgcc gtctgaaacg gtgcgacgca aatttcaatc agttttaaga
     8821 gcagacgaac aatggaacag cagcaaagat acatcagcgt actggatatc ggtacgtcta
     8881 aagtcctcgc actgatcggg gaagttcaag atgacgacaa aatcaacatc gtcggtttgg
     8941 ggcaggctcc ttcacggggc ttgcgcgcgg gcatggtaac caatatcgat gccaccgtcc
     9001 aagccatcag gcaggcggtc aatgatgccg agctgatggc ggataccaaa attactcacg
     9061 ttaccacagg tatcgcaggc aaccacatcc gcagtctcaa ttcgcaaggt gtggttaaaa
     9121 ttaaagatgg ggaagtcacg caggcagaca tcgatcgcgc cattgaaacg gcaaaggcaa
     9181 tcaatatccc gcccgatcaa aaaattctcg atgccgtggt tcaagactac attattgaca
     9241 cccaacttgg cgtgagggag cccatcggta tgagcggtgt gcgtctggat acgcgggtgc
     9301 acatcattac cggtgcaagt acggcagtgc agaatgtcca aaaatgtatc gagcggtgcg
     9361 gtttgaaaag cgatcagatc atgcttcagc cgttggcaag cgggcaggcg gtgctgactg
     9421 aagatgaaaa agacctcggc gtatgcgtca tcgacattgg tggcggaacg accgatattg
     9481 ccgtttatat gaacggtgcc atccgccata cgtccgtcat tccggccggt ggtaatctga
     9541 ttaccaaaga tttgtccaaa tcgttgagaa cacctctcga tgccgccgag tacattaaaa
     9601 tccattatgg cgtggcatca tgcgatacgg aaggcttggg tgagatgatt gaagttccgg
     9661 gcgtgggtga ccggacatcg cgtcaggttt ccagtaaggt tctggcagca atcatcagtg
     9721 cacggattca ggagattttt ggcgtagtgc tgggcgagct gcaaaaatcg ggtttcccca
     9781 aagaagtgct gaatgcgggt atcgttctga ccggcggtgt gtccatgatg accgggattg
     9841 tggaatttgc cgaaaaaatc ttcgatttgc ctgtacgcac cggtgcaccc caagaaatgg
     9901 gcggtttgtc cgaccgcgtc cgcacaccgc gtttttctac cgctatcggg ctgcttcatg
     9961 cagcatgcaa gctggaagga aacttgccgc agccggaaaa cggtgcagtg caagagaggg
    10021 aagggggcgg cggtttgttg gcaagattga aacggtggat tgaaaacagc ttctgaacag
    10081 gtggattgcc gtttgacagg tgagaagtat tttgccagca gcaagatact tcttatataa
    10141 tgaataataa tttatttaaa ccgtcctctg aatggggcga gcaggagttt ttgaatggaa
    10201 tttgtttacg acgtggcaga atcggcagtc agccctgcgg tgattaaagt aatcggcttg
    10261 ggcggcggcg gttgcaatgc aatcaataac atggttgcca acaatgtgcg cggtgtggag
    10321 tttatcagtg ccaatacgga tgcgcagtct ctggcaaaaa accatgcggc gaagagaatc
    10381 cagttgggta cgaatctgac acgcggtttg ggcgcgggcg cgaatcccga tatcggccgt
    10441 gcggcagccc aggaagaccg ggaagccatt gaagaagcca ttcgcggtgc gaatatgctg
    10501 tttatcacga ccggtatggg cggcggtacc ggtaccggtt ccgcgccggt tgttgctgag
    10561 attgccaagt ctttgggcat tctgaccgtt gccgtggtta cccgaccgtt cgcatatgaa
    10621 ggtaagcgcg tccatgtcgc acaggcaggg ttggaacagt tgaaagaaca cgtcgattcg
    10681 ctgattatca tcccgaacga caaactgatg actgcattgg gtgaagacgt aacgatgcgc
    10741 gaagccttcc gtgccgccga caatgtattg cgcgatgcgg tcgcaggcat ttccgaagtg
    10801 gtaacttgcc cgagcgaaat catcaacctc gactttgccg acgtgaaaac cgtgatgagc
    10861 aaccgcggta tcgctatgat gggttcgggt tatgcccaag gtatcgaccg tgcgcgtatg
    10921 gcgaccgacc aggccatttc cagtccgctg ctggacgatg taaccttgga cggagcgcgc
    10981 ggtgtgctgg tcaatattac gactgctccg ggttgcttga aaatgtccga gttgtccgaa
    11041 gtcatgaaaa tcgtcaacca aagcgcgcat cccgatttgg aatgcaaatt cggtgcggct
    11101 gaagacgaga ccatgagcga agatgccatc cggattacca ttatcgctac cggtctgaaa
    11161 gaaaaaggcg cggtcgattt tgttccggca agggaggtag aagcggttgc tccgtccaaa
    11221 caggagcaaa gccacaatgt cgaaggtatg atccgcacca atcgcggtat ccgcacgatg
    11281 aaccttaccg ctgcggattt cgacaatcag tccgtacttg acgactttga aatccctgcg
    11341 attttgcgtc gtcaacacaa ttcagacaaa taatgtgctg tttgcccgta aacctgctgc
    11401 ctcccgaatc ggtttgtccg gtttgggagg tatgtttttc aagatgttgc aatttcgtac
    11461 ggtttgcggt cggcggattc agatttttcc acttgataca gactttcaga tatggacact
    11521 tcaaaacaaa cactgttgga cgggattttt aagctgaagg caaacggtac gacggtgcgt
    11581 accgagttga tggcgggttt gacaactttt ttgacgatgt gctacatcgt tatcgtcaac
    11641 cctctgattt tgggcgagac cggcatggat atgggggcgg tattcgtcgc tacctgtatc
    11701 gcgtctgcca tcggctgttt tgttatgggt tttgtcggca actatccgat tgcactcgca
    11761 ccggggatgg ggctgaatgc ctatttcacc tttgccgtcg ttaagggtat gggcgtgcct
    11821 tggcaggttg cgttgggtgc ggtgttcatc tccggtctga tttttatcct gttcagcttt
    11881 tttaaagtca gggaaatgct ggtcaacgca ctgcctatgg gtttgaaaat gtcgattgct
    11941 gccggtatcg gtttgttttt ggcactgatt tccctgaaag gcgcaggcat tatcgttgcc
    12001 aatccggcaa ccttggtcgg tttgggcgat attcatcagc cgtccgcgtt gttggcattg
    12061 ttcggttttg ctatggtggt cgtattggga catttccgcg ttcaaggcgc aatcatcatc
    12121 accatcttga ccattaccgt cattgccagc ctgatgggtt tgaatgaatt tcacggcatc
    12181 atcggcgaag taccgagcat tgcgccgact tttatgcaga tggattttga aggcctgttt
    12241 accgtcagca tggtcagtgt gattttcgtc ttcttcttgg tcgatctatt tgacagtacc
    12301 ggaacgctgg tcggcatatc ccaccgtgcc gggctgctgg tggacggtaa gctgccccgc
    12361 ctgaaacgcg cactgcttgc agactctacc gccattgtgg caggtgcggc tttgggtact
    12421 tcttccacca cgccttatgt ggaaagcgcg gcgggcgtat cggcaggcgg acggaccggc
    12481 ctgacggcgg ttaccgtcgg cgtattgatg ctcgcctgcc tgatgttttc acctttggcg
    12541 aaaagtgttc ccgcttttgc caccgcgccc gccctgcttt atgtcggcac gcagatgctc
    12601 cgcagtgcga gggatattga ttgggacgat atgacggaag ccgcacctgc gttcctgacc
    12661 attgttttca tgccgtttac ttattcgatt gcagacggca tcgctttcgg cttcatcagt
    12721 tatgccgtgg ttaaactttt atgccgccgc accaaagacg ttccgcctat ggtatggatt
    12781 gttgccgtat tgtgggcact gaaattctgg tatttgggct gattgattcg atattaaaaa
    12841 tgccgtctga aaggttttca gacggcattt tgtttgccga tatatttaat ttttattaaa
    12901 ttatataaaa atcaaataca taataaaata catcggattg cttaaaaata atacattgtt
    12961 tttatgtata aaatatttta taagttttca ggattttgat tatcaaaaat ttttcttgat
    13021 ttcctgacaa ttttattgaa acaaataatt caaaattaat ctagtttaat catggaatta
    13081 aaataaaata ttaaaattat gtaatgagtc tccttaaaaa tgtttgacat tttcagtctt
    13141 gtgttttaga ttatcgaaaa ataaaactac ataacactac aaaggaacat tactatgaaa
    13201 ccaattcaga tgttttcccc ttttctgaat aatccccttg ttttcttctt gtctgcggtt
    13261 ttgccgcata attccgaacg gtctgctgtt tttctttgat tcgttttaaa tatcaataag
    13321 ataatttttc ccatatattt ttaatgattg gattgggatg cccgacgcgt cggatggctg
    13381 tgttttgccg tccgaatgtg atggaagcct gtccatactg aaaaaaagtc tataaaggag
    13441 aaatatgatg agtcaacact ctgccggagc acgtttccgc caagccgtga aagaatcgaa
    13501 tccgcttgcc gtcgccggtt gcgtcaatgc ttattttgca cgattggcca cccaaagcgg
    13561 tttcaaagcc atctatctgt ccggcggcgg cgtggcagcc tgttcttgcg gtatccctga
    13621 tttgggcatt accacaatgg aagatgtgct gatcgacgca cgacgcatta cggacaacgt
    13681 ggatacgcct ctgctggtgg acatcgatgt gggttggggc ggtgcattca atattgcccg
    13741 taccattcgc aactttgaac gcgccggtgt tgcagcggtt cacatcgaag atcaggtagc
    13801 gcaaaaacgc tgcggccacc gtccgaacaa agccattgta tctaaagatg aaatggtcga
    13861 ccgtatcaaa gctgccgtag atgcgcgcgt tgatgagaac ttcgtgatta tggcgcgtac
    13921 cgatgcgctg gcggtagaag gtttggatgc cgctatcgaa cgcgcccaag cttgtgtcga
    13981 agccggtgcg gacatgattt tccctgaagc catgaccgat ttgaacatgt accgccaatt
    14041 tgcagatgcg gtgaaagtgc ccgtgttggc gaacattacc gagtttggtt ccactccgct
    14101 ttatacccaa agcgagctgg ctgaaaacgg cgtgtcgctg gtgctgtatc cgctgtcatc
    14161 gttccgtgca gcaagcaaag ccgctctgaa tgtttacgaa gcgattatgc gcgatggcac
    14221 tcaggcggcg gtggtggaca gtatgcaaac ccgtgccgag ctgtacgagc atctgaacta
    14281 tcatgccttc gagcaaaaac tggataaatt gtttcaaaaa tgatttaccg ctttcagact
    14341 gcctttcaac aaatccgcat cggtcgtctg aaaacccgaa acccataaaa acacaaagga
    14401 gaaataccat gactgaaact actcaaaccc cgaccctcaa acctaaaaaa tccgttgcgc
    14461 tttctggcgt tgcggccggt aataccgctt tgtgtaccgt tggccgtacc ggcaacgatt
    14521 tgagctatcg cggttacgac attctggatt tggcacaaaa atgcgagttt gaagaagtcg
    14581 cccacctgct gattcacggc catctgccca acaaattcga gctggccgct tataaaacca
    14641 agctcaaatc catgcgcggc ctgcctatcc gtgtgattaa agttttggaa agcctgcctg
    14701 cacataccca tccgatggac gtaatgcgta ccggcgtatc catgctgggc tgcgttcatc
    14761 ctgaacgtga aagccatccg gaaagtgaag cgcgcgacat cgccgacaaa ctgatcgcca
    14821 gcctcggcag catcctcttg tactggtatc aatattcgca caacggcaaa cgcattgagg
    14881 ttgaaagcga cgaagagacc atcggcggtc atttcctgca actgttgcac ggcaaacgcc
    14941 caagcgaatc acacatcaaa gccatgcacg tttcactgat tctgtatgcc gaacacgagt
    15001 tcaacgcttc tacctttacc gcccgcgtga tcgccggtac aggctctgat atgtactcca
    15061 gcattaccgg agcaatcggc gcgttgaaag gtccgaaaca cggcggcgcg aacgaagtgg
    15121 cttacgatat tcaaaaacgc taccgcaatg ccgacgaagc tgaagccgac atccgcgaac
    15181 gcatcggccg caaagaaatc gtgatcggtt tcggtcatcc ggtgtacacc atttccgacc
    15241 ctcgcaacgt tgtcattaaa gaagtggcac gcggtttgag caaagaaacc ggcgatatgc
    15301 gcctctttga cattgccgaa cgtttggaaa gcgtgatgtg ggaagagaaa aaaatgttcc
    15361 cgaatctgga ctggttctct gccgtttcct accaaaaatt gggcgtaccg accgctatgt
    15421 tcacaccgct gttcgtaatt tcccgtacaa ccggttggag cgcacacgtt cttgagcaac
    15481 gcaaagacgg caaaatcatc cgtccgagcg caaactacac aggccctgaa gatttggcgt
    15541 ttgtggagat tgaagaacga taattgaaga atgcaatagc agtttgttct ttaatttcgg
    15601 tatgcaaagc taaggatttc agacgacctt gccttattgg aaaggttgtc tgaaataagt
    15661 ttaatctaat aggagaagat aatcctgtat tggcgcaagt aacaggataa gaaacatgga
    15721 agatttatat ataatactcg ctttgggttt ggttgcgatg attgccggat ttatcgatgc
    15781 gattgcgggc gggggtggtt tgattacgct gcccgcactc ttgttggcag gtattcctcc
    15841 cgtgtcggca attgccacca acaagctgca agcagccgct gctacgtttt cagctacggt
    15901 ttcttttgca cgcaaaggtt tgattgattg gaagaaaggt ctcccgattg ccgcagcatc
    15961 gtttgtaggc ggcgtggccg gtgcattatc ggtcagcttg gtttccaaag atattctgct
    16021 ggcggtcgtg ccggttttgt tgatatttgt cgcactgtat tttgtgtttt cgcccaagct
    16081 cgacggcagt aaggaaggca aagccagaat gtcttttttt ctgttcgggc tgacggtcgc
    16141 accgcttttg ggtttttacg acggtgtgtt cggaccgggt gtcggctcgt tttttctgat
    16201 tgcctttatt gttttgctcg gctgcaagct gttgaacgcg atgtcttaca ccaaattggc
    16261 gaacgttgcc tgcaatcttg gttcgctatc ggtattcctg ctgcacggtt cgattatttt
    16321 cccgattgcg gcaacgatgg cggtcggtgc gtttgtcggt gcgaatttag gtgcgagatt
    16381 tgccgtccgc ttcggttcga agctgattaa gccgctgctg attgtcatca gcatttcgat
    16441 ggctgtgaaa ttgttgatag acgagagaaa tccgctgtat cagatgattg tttcgatgtt
    16501 ttaaaccctt tcagacgacc ccttcaaaac gtcggctgaa acctcaaacc acaagaaaaa
    16561 cagatccaca ggagaaccga catggctgcc aaccaacgtt accgcaaacc gctgcccggt
    16621 acggatttgg aatactacga cgcgcgtgcg gcgtgtgagg acatcaagcc cggctcttac
    16681 gacaagctgc cttacacgag ccgcattttg gcggagaatt tggtcaaccg cgcggacaaa
    16741 gtcgatttgc cgacgctgca aagctggctg gggcagttga tagaagggaa gcaggaaatc
    16801 gactttccgt ggtatccggc gcgggtggtg tgccacgata ttctggggca gaccgcgttg
    16861 gtggatttgg caggcctgcg cgatgcgatt gccgaaaaag gcggcgatcc tgccaaagtg
    16921 aatccggtgg tgcaaaccca gctcatcgtc gaccactctc tggccgttga atgcggcggc
    16981 tacgaccccg atgccttccg caaaaaccgc caaatcgaag acagacgtaa cgaagaccgt
    17041 ttccacttca tcaactggac aaaaaccgca tttgaaaatg tggacgtgat tccggcgggc
    17101 aacggcatca tgcaccaaat caatctagaa aaaatgtcgc ccgtcgtcca agtcaaaaac
    17161 ggcgtggcgt tccccgatac ctgcgtcggc acggattcgc acacgccgca cgtcgatgcg
    17221 ctgggcgtga tttccgtggg cgtgggcgga ttggaagcgg aaaccgtgat gctgggtcgc
    17281 gcgtccatga tgcgcctgcc cgatattgtc ggcgttgagc tgaacggcaa acggcaggcg
    17341 ggcattacgg cgacggatat tgtgttggca ctgaccgagt ttctgcgcaa agagcgcgtg
    17401 gtcggggcgt ttgtcgaatt cttcggcgag ggcgcgagaa gcctgtctat cggcgaccgc
    17461 gcgaccattt ccaacatgac gccggagttc ggcgcgactg ccgcgatgtt cgctattgat
    17521 gagcaaacca ttgattattt gaaactgacc ggacgcgacg acgcgcaggt gaaattggtg
    17581 gaaacctacg ccaaaaccgc aggcttatgg gcagatgcct tgaaaaccgc cgtttatcct
    17641 cgcgttttga aatttgattt gagcagcgta acgcgcaata tggcaggtcc aagtaacccg
    17701 catgcccgtt ttgcgaccgc cgatttggcg gcgaaagggc tggcgaagcc ttacgaagag
    17761 ccttcggacg gccaaatgcc cgacggcgcg gtcatcatcg ccgcgattac cagttgcacc
    17821 aacacttcca acccgcgcaa cgtcgttgcc gccgcgctct tggcgcgcaa cgccaaccgc
    17881 ttcgggctga aacgcaaacc ttgggtgaaa tcttcgtttg ccccgggttc aaaagtagcc
    17941 gaaatttatt tgaaagaagc aggcctgctg cccgaaatgg aaaaactcgg cttcggtatc
    18001 gtcgccttcg cctgcaccac ctgcaacggc atgagtggcg cgctggatcc gaaaatccag
    18061 aaagaaatca tcgaccgcga tttgtacgcc accgccgtat tatcaggcaa ccgcaacttc
    18121 gacggccgtg tccatccgta tgcgaaacag gctttcctcg cttcgcctcc gttggtcgtt
    18181 gcctacgcgc tggcaggcag tatccgtttc gatattgaaa acgacgtact cggcgttgca
    18241 gacggcaagg aaatccgcct gaaagacatt tggcctgccg atgaagaaat cgatgccgtc
    18301 gttgccgaat atgtgaaacc gcagcagttc cgcgatgtgt atgtaccgat gttcgacacc
    18361 ggcacagcgc aaaaagcacc tagtccgctg tacgactggc gtccgatgtc cacctatatc
    18421 cgccgcccac cttactggga aggcgcactg gcaggggaac gcacattaag aggtatgcgt
    18481 ccactggcga ttttgcccga caacatcacc accgaccacc tctcgccgtc caatgcgatt
    18541 ttggccgtca gtgccgcagg cgagtatttg gcgaaaatgg gtttgcctga agaagacttc
    18601 aactcttacg caacccaccg tggcgaccac ttgaccgccc aacgcgctac cttcgccaat
    18661 ccgaaactgt ttaacgaaat ggtgagaaac gaagatggca gcgtacgcca aggttcgctg
    18721 gcacgcgttg aacccgaagg taaaaccatg cgcatgtggg aagccatcga aacctatatg
    18781 aaccgcaaac agccgctcat catcattgcc ggtgcggact acggtcaagg ctcaagccgc
    18841 gactgggctg caaaaggcgt acgcctcgcc ggcgtggaag cgattgttgc cgaaggcttc
    18901 gagcgtatcc accgcaccaa cttgatcggt atgggcgtgt tgccgttgca gttcaaaccg
    18961 ggtaccaacc gccataccct gcaactggac ggtacggaaa cctacgacgt tatcggcgaa
    19021 cgtacaccgc gctgcgacct gacccttgtg attcaccgta aaaacggaga aaccgtcgaa
    19081 gttccggtta cctgccgcct cgatactgca gaagaagtat tggtatatga agccggcggc
    19141 gtgttgcaac ggtttgcaca ggattttttg gaagggaacg cggcttagag gtcgtctgaa
    19201 aagcaagacg tagcgtgggt cgggttcaac attttgctca ttcacgtaat tctcgatatg
    19261 gcaggcatct actgtaaatc gtcattcccg cgcaggcggg aatccagaaa gtggaattga
    19321 ggaaacctta tttatccgat gagtttctgt gcggataaat ctggattccc gcctgcgcgg
    19381 gaatgacggg gtttaataat ctgccgtatc acaacacagt agccgtagat tgtggcgaac
    19441 cccgacagtt tgcggaatca aacggctttg gtcggagtgg cagcctaatc cactataaaa
    19501 atcgtgggca gagcccacgc tacataagga gaatctagaa atgccgcaaa ttaaaatccc
    19561 cgccgtttac taccgcggtg gcacatcaaa aggcgtgttt ttcaaacgtt ccgacctgcc
    19621 cgaggcggcg cgggaagcgg gaagcgcacg cgacaaaatc ctcttgcgcg tactcggcag
    19681 cccggatccc tacggcaagc agatagacgg tttgggcaac gctagctcgt ccaccagcaa
    19741 ggcggtgatt ttggacaagt ccgaacgcgc cgatcacgat gtcgattacc ttttcgggca
    19801 agtttccatc gacaaacctt ttgtcgattg gagtggcaac tgcggcaacc tcaccgccgc
    19861 cgtgggcgca tttgccatcg agcaaggctt ggtcgataaa ggcaagattc cttcagacgg
    19921 catctgcacg gtcaaaatct ggcagaaaaa catcggcaaa accattattg cccatgtacc
    19981 gatgcaaaac ggcgcagttt tggaaacagg cgattttgag ctcgacggcg taacgttccc
    20041 ggcagccgaa gtacaaatcg aatttcttga tccagccgac ggtgaaggca gtatgttccc
    20101 aaccggcaat ttggtcgatg aaattgatgt gccgaatata ggccgtttga aagccacgct
    20161 catcaacgcg ggcattccga ccgttttcct gaatgccgcc gacttgggct acacgggcaa
    20221 agagttgcaa gacgacatca acaacgatgc cgcggctttg gaaaaattcg agaaaatccg
    20281 cgcttacggt gcgctgaaaa tgggtctgat cagcgacgta tccgaagctg ccgctcgcgc
    20341 gcacacgccg aaagtcgcct tcgtcgcgcc cgccgccgat tacaccgcct ccagtggcaa
    20401 aaccgtgaac gccgccgaca tcgatttgct ggtacgcgcc ctgagcatgg gcaaattgca
    20461 ccacgcgatg atgggtaccg cctctgttgc cattgcgacc gccgccgccg tgcccggtac
    20521 gctggtcaac cttgccgcag gcggcggaac gcgtaaagaa gtgcgcttcg ggcatccttc
    20581 cggcacattg cgcgtcggtg cagccgccga atgtcaggac ggacaatgga cggccaccaa
    20641 agcggttatg agccgcagcg cacgcgtgat gatggaaggt tgggtcaggg tgccggaaga
    20701 ttgtttttaa attgacgtag catgggtttg cccgcgagcc ataaacaggt cgtctgaaaa
    20761 acaagtaaac atcaaatcac tgaccattcc tttcccttgc cctgtggcgg aaggcggcaa
    20821 atcacaagga agaacacgga aaccccgata aaagacagct tcccgtatta ccgtcattcc
    20881 cgcgcaggcg ggaatctaga cctgtcaata tggaggattg gtaggggaaa acaggtttcg
    20941 tgagttctac attctggatt cccgccacag cctgtcctcg cgtaggcggg gacggaataa
    21001 cgatagaaaa tgcggcatac gctttgccca aagaggccgt ctgaaacacc ttgcgcctga
    21061 tgtctgcctt tttcagacga ccccacacca aaaaaacaac cacaaactac aaggagaaac
    21121 atcatgtccg accaactcat cctcgttctg aactgcggca gttcatcgct caaaggcgcc
    21181 gttatcgacc gaaaaagcgg cagcgtcgtc ctaagctgcc tcggcgaacg cctgaccacg
    21241 cccgaagccg tcattacgtt caacaaagac ggcaacaaac gccaagttcc cctgagcggc
    21301 cgaaattgcc acgccggcgc ggtgggtatg cttttgaacg aactggaaaa acacggtctg
    21361 cacgaccgca tcaaagccat cggccaccgc atcgcccacg gcggcgaaaa atacagcgag
    21421 tctgttttga tcgaccaggc cgtaatggac gaactcaatg cctgcattcc gcttgcgccg
    21481 ctgcacaacc ccgccaacat cagcggcatc cttgccgcac aggaacattt ccccggtctg
    21541 cccaatgtcg gcgtgatgga tacttcgttc caccaaacca tgccggagcg tgcctacact
    21601 tatgccgtgc cgcgcgagtt gcgtaaaaaa tacgctttcc gccgctacgg tttccacggc
    21661 accagtatgc gttacgttgc ccctgaagcc gcacgcatct tgggcaaacc tctggaagac
    21721 atccgcatga ttattgccca cttaggcaac ggcgcatcca ttaccgccat caaaaacggc
    21781 aaatccgtcg ataccagtat gggtttcacg ccgatcgaag gtttggtaat gggtacacgt
    21841 tgcggcgaca tcgatccggg cgtatacagc tatctgactt cccacgccgg gatggatgtt
    21901 gcccaagtgg atgaaatgct gaacaaaaaa tcaggtttgc tcggtatttc cgaactttcc
    21961 aacgactgcc gcaccctcga aatcgccgcc gacgaaggcc acgaaggcgc gcgcctcgcc
    22021 ctcgaagtca tgacctaccg cctcgccaaa tacatcgctt cgatggctgt ggcctgcggc
    22081 ggcgttgacg cactcgtgtt caccggcggt atcggcgaaa actcgcgtaa tatccgtgcc
    22141 aaaaccgttt cctatcttga tttcttgggt ctgcacatcg acaccaaagc caatatggaa
    22201 aaacgctacg gcaattcggg cattatcagc ccgaccgatt cttctccggc tgttttggtt
    22261 gtcccgacca atgaagaact gatgattgcc tgcgacactg ccgaacttgc cggcatcttg
    22321 tagccaaaaa agggacgagt ccgcaaaaat gccgtctgaa attttcagac ggcattttgt
    22381 ttggattttg ggcgtacaat acggaaaaca tgacgataag gacacaaacc atggcacagt
    22441 ttttcgctat tcatcccgac aatccccaag aacgcctcat caagcaggcg gttgaaatcg
    22501 tcaatcaagg cggcgtggtg gtttatccga ccgattcgtg ttacgccttg ggttgcaaac
    22561 tcggcgataa ggcggcgatg gaacgcatac tctccatccg caaaatcgat ttgaaacacc
    22621 acctgaccct gatgtgcgcc gatttgagcg agttgggcac gtacgccaaa attgacaacg
    22681 tacagtttcg tcagcttaaa gccgccacgc ccgggcctta tacctttatc ttgcaggcga
    22741 cgaaggaagt gccggcgcgc acgctgcacc cgaaacgcaa aaccatcggg ctgcgtattc
    22801 ccgataatgc cattgcacaa gccctgctgg gggaattggg cgagccgctt ttaagctgca
    22861 ccctgatgct gcccgaagac ggcgaaccat tgaccgatcc ttatgaaatc cgcgagcgtt
    22921 tggaacacgc cgtcgatttg gtgattgacg gcggctggtg cggaaccgag ccgaccaccg
    22981 tcgtcgatat gaccgacggc acggaactgg tgcgccgggg ttgtggcgat acggcggtgt
    23041 tcggtttgta gggaaaccga tgccgtctga agcatcggct gttcagacgg cattgcgcgc
    23101 cttgccggcg gcagtccgaa atgccggcgc gtatcgcgct cggtcggaat atccgtttga
    23161 aacggcattt tgatgcatta ctgcaccgca atcggaattc tcggttcgta gagcaggtcg
    23221 taggtcggct tgttgagcag gtcttggagc gtgaaaccgt ccagatacgt gaaaaacgac
    23281 ttcattgcac cgccgagtat gcctgtcaga cggcaggacg gtgtaatcag gcattcgttg
    23341 ttcgaaccca tacattcgac cagctgcatc ggttcgaggt ggcggacgac cgagccgatg
    23401 ttgatgcggt cgggcggtgt ggtaagccgc agaccgccgc cttttccgcg cacactgtgg
    23461 aggaagccgc ctttgaccag cgcggtaacg accttcatca gatggctttt ggaaatgccg
    23521 taggttacgg cgatggtact gatgttgacc agcgcatcgt cgttgatggc agtgtagata
    23581 aggacgcgca gcccgtagtc cgtatgttgt gtcaaataca tgattttctc ggtatggatt
    23641 gttattctta tcggtacggt ttaaggttca cggacaatac cttaatggtt gaaaccctgt
    23701 ccgtcggggc ggtagaatgc agcctgtctg cggcggtatg ccgtctgaaa catccgcgct
    23761 accgtttgag aatttgttat tgtaactcaa aatcatgaaa ccgttgaaac gacatcccgc
    23821 ccttatcggg ctttcgcgtg accaccacca ttcgctttcc ttgtgcgtgc gtctgttgcg
    23881 gacgccggaa gaaaggcatc gggacgaact cgaaccgcat ttttccgaat tggaaaccca
    23941 ttttcgcgaa gaagaaacca agtttgcccc aatttggcag aatgtcgccc ccgaattgaa
    24001 acaacgtttc gagaaagacc acgcccgact gcggcagatg atggcaagcc ccgaatacgg
    24061 taacgcggcg tggaataccg cttttgccac aaccctgcgc gaccacgcgc gctttgaaga
    24121 acgcgagctg tttcccgccg ccgaaccgtt tttgccggca tgattccgtt ttgcggcaaa
    24181 tatattaatg ataaacaagg aacacacatg aaatttacca agcaccccgt ctgggcaatg
    24241 gcgttccgcc cgttttattc gctggcggct ctgtacggcg cattgtccgt attgctgtgg
    24301 ggtttcggct acacgggaac gcacgagctg tccggtttct attggcacgc gcatgagatg
    24361 atttggggtt atgccggact ggtcgtcgtc gccttcctgc tgaccgccgt cgccacttgg
    24421 acggggcagc cgcccacgcg gggcggcgtt ctggtcggct tgactatctt ttggctggct
    24481 gcgcggattg ccgcctttat cccgggttgg ggtgcgtcgg caagcggcat actcggtacg
    24541 ctgtttttct ggtacggcgc ggtgtgcatg gctttgcccg ttatccgttc acagaatcaa
    24601 cgcaactatg ttgccgtgtt cgcgctgttc gtcttgggcg gcacgcatgc ggcgttccac
    24661 gtccagctgc acaacggcaa cctaggcgga ctcttgagcg gattacagtc gggcttggtg
    24721 atggtgtcgg gttttatcgg tctgattggt acgcggatta tttcgttttt tacgtccaaa
    24781 cggttgaatg tgccgcagat tcccagtccg aaatgggtgg cgcaggcttc gctgtggctg
    24841 cccatgctga ccgccatgct gatggcgcac ggcgtgatgc cttggctgtc ggcggctttc
    24901 gcgtttgcgg caggcgtgat ctttaccgtg caggtgtacc gctggtggta taagcctgtg
    24961 ttgaaagagc cgatgctgtg gattctgttt gccggctatc tgtttaccgg attggggctg
    25021 attgcggtcg gcgcgtctta tttcaaaccc gctttcctca atctgggtgt gcatctgatc
    25081 ggggtcggcg gtatcggcgt gctgactttg ggcatgatgg cgcgtaccgc gctcggtcat
    25141 acgggcaatc cgatttatcc gccgcccaaa gccgttcccg ttgcgttttg gctgatgatg
    25201 gcggcaaccg ccgtccgtat ggttgccgta ttttcttccg gcactgccta cacgcacagc
    25261 atacgcacct cttcggtttt gtttgcactc gcgcttttgg tgtatgcgtg gaagtatatt
    25321 ccttggctga ttcgtccgcg ttcggacggc aggcccggtt gagacaaacc gccgcagatt
    25381 tcgggtctgg gcggtttgct tttcagacgg cagggcggtc agttgccgtc cagccagcgg
    25441 tcgcgtgtgg ttttggcctc ttcaaaatag cggtacaggg cttcgcggtc gtcggtggtc
    25501 aggatgtttg ccaaaacgtc caactgtttg cccaagcctt gaaccagttg cagcaggctg
    25561 tctttgttgg caaggcagat gtccgcccac acggcgggat ggccggaggc gatgcgggtg
    25621 aagtcccgaa agcccgtggc ggcgaatttc agatattcct gtccgtcggg gtggtcgaga
    25681 atctggtgga cataggcgaa ggcggtcagg tggggcatat gggagacggc ggcgaaaacc
    25741 gcgtcgtggc gttgcgcgtc catcgtataa atttccgcac cgaccgcgtg ccacaggttt
    25801 tctatcaagg caatgccgtc tgaatgttcg ccgccgtgtg gcgtgatgat gagttttctg
    25861 tggcggaaca gcccgaactg cgcggcttgc gcaccgcttc tgtccgaacc ggcaattggg
    25921 tgggcggcga tgcagtggtg caggcggtcg ggcagacagc ggcggaaggc ttcgatgacc
    25981 gaagatttgg tgctgccgac atcggaaatc caagtgtgtt ccggcaaaac ggggcgcagc
    26041 gcggtcaaaa tggcgggaac ggtggcgacg ggcgtggcaa tcagtaccaa gtccgcaccg
    26101 ccgatgctgt ccgcgtcgat ggcaacggaa gcccggtcaa tcacgccgcg ttccaatgca
    26161 cgttcgaggt tgtcgcggtc ggtgtcgata ccggtaacgg tgcggacgag tccctgcctt
    26221 ttgaggtcga gaacgaacga accgccgatc agccctacac cgatgagggc aatatggttc
    26281 aaaatgggca tttgtgtaaa cggttttcgc aaagtaccgt catggtagcc tatcggcgga
    26341 atatgccgca aggtcggcag gaaaaaggag aagaaatgga caaaatcaga gttgccgccg
    26401 tgcagatggt gtcgggcgtg tcgccggaaa ccaacgtcgc tgccatgaaa cgcctggtcg
    26461 cacgggcggc ggagcagggg gcggattggg tgctgctgcc cgaatattgg gtgctgatgg
    26521 gcgcaaacga taccgacaaa ctcgcgcttg ccgagccttt gggcggcgga cgctttcaga
    26581 cggcattgag cgaaacggcg aaagaatgcg gcgtggtgct gtttggcgga actgtgccgc
    26641 tgcaaagcag tgaggcaggc aaggtgatga atacactgtt ggtgtacgga cgggacggcg
    26701 taagaacggg tctgtaccac aaaatgcacc tcttcggctt ttccggtttg ggcgaacgct
    26761 atgccgaagc cgataccatc agcgcagggc gggcagtgcc gcacttgtcg gcagagggcg
    26821 tgccggtggc ggcaggtatt tgttacgatg tccgctttcc cgaatttttc cgccgccagc
    26881 tgccgtttga cgtattgatg cttccggcgg catttaccca tacgacgggc aaggcgcact
    26941 gggagctgct tttgcgtacg cgtgccgtcg aaaaccaatg ttatgttgtg gcggcggcac
    27001 agggcggatt gcacgaaaac ggcaggcgca cgttcggaca cagtatgatt gtcgatccgt
    27061 ggggcgacgt gttggacgta ttgcccgaag gcgaaggcat cgttacggca gacattgatg
    27121 ccaaccgcct gaacagcgtc cgcaaccgcc tgcccgccct gaaataccgg gttttggatg
    27181 ccgtctgaag gttcagacgg catcggtgcc ggggaatcag cgggctgtag gatgggcttc
    27241 agcctgccat ttccatcaat cccacatttc taccgttttc atcgaatcca tcgaatccgc
    27301 ccctttcgac gacccagtcc tacgcaactg aa
//
LOCUS       DAXHNE010000012        26664 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.012, whole genome shotgun sequence.
ACCESSION   DAXHNE010000012 DAXHNE010000000
VERSION     DAXHNE010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26664
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.012"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(231..1028)
                     /locus_tag="ACFM4Z_000379"
     CDS             complement(231..1028)
                     /locus_tag="ACFM4Z_000379"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFC6020480.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSICCGWWSLPFWVFTV
                     FALQFFRDPAREIPLNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVNGVSTVLARLPLTAP
                     QIESEPESEPASQAASVETAANPSAEQRQIEATAAKIQAAVQDVLKD"
     gene            1183..4032
                     /gene="uvrA"
                     /locus_tag="ACFM4Z_000380"
     CDS             1183..4032
                     /gene="uvrA"
                     /locus_tag="ACFM4Z_000380"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFC6020481.1"
                     /translation="MCNHHPQHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPTVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLASGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCENSVTGQYLSGKKSIAVPSERTPVNPDRMLIL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIHGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIAKGSPEQVAKVKGSYTGKYLKVVLR"
     gene            4066..5661
                     /locus_tag="ACFM4Z_000381"
     CDS             4066..5661
                     /locus_tag="ACFM4Z_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="funZ protein"
                     /protein_id="HFC6020482.1"
                     /translation="MKPLKTLEFGFVDAANYRRRENKDLFNRIFVKGEYLDELCEPNI
                     SFLIGEKGTGKTAYAVYLTNNFYKNIHATTKFVRETDYSKFIQLKKARHLTVSDFTSI
                     WKVILYLLISNQIKCKENGILSSIFNKFKALDEAINEYYYGAFDPEIVQAITLIENSK
                     EAAEMIFGKFVKLGEEESQQITFTESKFQANLGFIERKFKDALSQLKLKDNHILFIDG
                     IDIRPSQIPFDEYHECVKGLANAIWMLNNDIFPSIKDSKGRMRVVLLIRPDIFDSLGL
                     QNQNTKLQDNSVFLDWRTDYKSYRSSKIFGVFDHLLRTQQEKQDSLEKGNSWDYYFPW
                     NAPNLHDEYKNLTSFISFLRKSYYRPRDILQMLTLLQKNKKSKEDYVVAEDFDNTSFQ
                     REYSIYLLGEIKDHLLFYYSQSDYQNFLKFFEFLNGKDRFKYSDFLKAFERLKKHLQT
                     TSVEIPKFMSTANEFLQFLFDLNVIAYLDNPEDETKPYIHWCFKDRNYANISPKIKTE
                     TEYLIFSGLSKALDVGTPFKNKQ"
     gene            complement(6141..7031)
                     /gene="sucD"
                     /locus_tag="ACFM4Z_000382"
     CDS             complement(6141..7031)
                     /gene="sucD"
                     /locus_tag="ACFM4Z_000382"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFC6020483.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVSKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(7042..8253)
                     /gene="sucC"
                     /locus_tag="ACFM4Z_000383"
     CDS             complement(7042..8253)
                     /gene="sucC"
                     /locus_tag="ACFM4Z_000383"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFC6020484.1"
                     /translation="MEADSGHSTNLKEKSMNLHEYQAKELLASYGLPVQGGILAHNGE
                     EAAAAYDKLGGKFAVVKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTD
                     ANGQPVNSVLVCEDMYPVQTELYLGAVVDRSTRRITFMASTEGGVEIEKVAAETPEKI
                     FKVTVDPLVGLQPCQAREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLA
                     VRENGALACVDGKIGIDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIG
                     CMVNGAGLAMATMDIIKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVQGVLINI
                     FGGIVRCDMIAEAIVAAVKEINVNVPVVVRLEGNNAELGAKILNESGLKLTSADGLND
                     AAEKIVAAVNA"
     gene            complement(8307..8594)
                     /locus_tag="ACFM4Z_000384"
     CDS             complement(8307..8594)
                     /locus_tag="ACFM4Z_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020485.1"
                     /translation="MNKEIVGIFFIPAGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            complement(8719..10152)
                     /gene="lpdA"
                     /locus_tag="ACFM4Z_000385"
     CDS             complement(8719..10152)
                     /gene="lpdA"
                     /locus_tag="ACFM4Z_000385"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6020486.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            10349..10462
                     /locus_tag="ACFM4Z_000386"
                     /pseudo
     CDS             10349..10462
                     /locus_tag="ACFM4Z_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(10517..11716)
                     /gene="odhB"
                     /locus_tag="ACFM4Z_000387"
     CDS             complement(10517..11716)
                     /gene="odhB"
                     /locus_tag="ACFM4Z_000387"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFC6020487.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAATAAAEAPAAAPA
                     EAAPAAAPAAAQNNAAMPAAAKLAAESGVDVNALQGSGRDGRVLKEDVQNAAAKPAPA
                     VALPAGARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKF
                     EKEHGVKLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVP
                     ILRDADQMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINP
                     PQSAILGMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDP
                     ARLLLDL"
     gene            complement(11816..14644)
                     /locus_tag="ACFM4Z_000388"
     CDS             complement(11816..14644)
                     /locus_tag="ACFM4Z_000388"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFC6020488.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGSADEAMLKKQVSVLRLISAYRIQG
                     VGAAKLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(14849..16132)
                     /gene="gltA"
                     /locus_tag="ACFM4Z_000389"
     CDS             complement(14849..16132)
                     /gene="gltA"
                     /locus_tag="ACFM4Z_000389"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFC6020489.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSRVAEYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            complement(16244..16492)
                     /locus_tag="ACFM4Z_000390"
     CDS             complement(16244..16492)
                     /locus_tag="ACFM4Z_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HFC6020490.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(16496..17203)
                     /locus_tag="ACFM4Z_000391"
     CDS             complement(16496..17203)
                     /locus_tag="ACFM4Z_000391"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HFC6020491.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(17323..19086)
                     /gene="sdhA"
                     /locus_tag="ACFM4Z_000392"
     CDS             complement(17323..19086)
                     /gene="sdhA"
                     /locus_tag="ACFM4Z_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFC6020492.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPADAGELTRQRIERLDNQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDANTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(19089..19430)
                     /gene="sdhD"
                     /locus_tag="ACFM4Z_000393"
     CDS             complement(19089..19430)
                     /gene="sdhD"
                     /locus_tag="ACFM4Z_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HFC6020493.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(19424..19801)
                     /gene="sdhC"
                     /locus_tag="ACFM4Z_000394"
     CDS             complement(19424..19801)
                     /gene="sdhC"
                     /locus_tag="ACFM4Z_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HFC6020494.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(20080..21483)
                     /locus_tag="ACFM4Z_000395"
     CDS             complement(20080..21483)
                     /locus_tag="ACFM4Z_000395"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6020495.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(21732..22469)
                     /locus_tag="ACFM4Z_000396"
     CDS             complement(21732..22469)
                     /locus_tag="ACFM4Z_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HFC6020496.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(22748..25024)
                     /gene="metE"
                     /locus_tag="ACFM4Z_000397"
     CDS             complement(22748..25024)
                     /gene="metE"
                     /locus_tag="ACFM4Z_000397"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFC6020497.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADYVAAGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHANTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALTVDLPKEWV
                     EAYKDVYATLSKVSAKILLSTYFGSVAEHAALLKSLPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGIIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTHKLRAELAK"
     gene            complement(25161..26039)
                     /gene="metF"
                     /locus_tag="ACFM4Z_000398"
     CDS             complement(25161..26039)
                     /gene="metF"
                     /locus_tag="ACFM4Z_000398"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFC6020498.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            26241..26516
                     /locus_tag="ACFM4Z_000399"
     CDS             26241..26516
                     /locus_tag="ACFM4Z_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFC6020499.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            26516..26641
                     /gene="ykgO"
                     /locus_tag="ACFM4Z_000400"
     CDS             26516..26641
                     /gene="ykgO"
                     /locus_tag="ACFM4Z_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFC6020500.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
BASE COUNT         6683 a         6759 c         6459 g         6763 t
ORIGIN      
        1 aaaggtttct gttttttaag ttttaagtaa ctaatgagtt gtcattcccg cgcagacggg
       61 aatccaattc gttcggtttc agccgtttcc gataaatcgc tgtaacgtta agttcccgga
      121 ttcccatttt cgtgaacata acggtgtgaa cattagcaaa aattcaaaaa accaaacttc
      181 ctcattcgtg tgaatgatgg tactgatatt ttctatttta gttcgcaaaa ttaatctttc
      241 aacacatctt gcacagccgc ttgaatctta gccgccgttg cttcaatttg ccgttgttcg
      301 gcagatgggt ttgccgctgt ttcaaccgaa gccgcttgtg aagctggctc agattcaggc
      361 tcggattcga tttgcggcgc agtcagcggc aaacgcgcca atacagtgct gacgccgttt
      421 actttatcgc caatcgccac ttgcgcctgc gcatcgacag gcagatacat atccacgcgc
      481 gagccaaaac ggataaagcc atagcgttcg ccgcgagaca gtttcgcacc tgcttgggtg
      541 tagcacaaaa tacggcgcgc caccaaaccg gccacttgaa caaaagtaat ttcacgacct
      601 gaagccgtag tcgccaacac cgcattacgt tcgttttccg tgctggcttt gtccaaatcc
      661 gcattcacga atttgccttt gttatagacc actttcgtta ccgtacaatc ggcaggcgat
      721 ttttgcgaat gcacgttgaa cacgttcata aaaatactga ttttcaaagc atcgacatca
      781 cgatacggat cgcgtgcgcg ttcgaccacc acgatacggc cgtcaaccgg gctcaacacc
      841 gcttcaggat ttagcggaat ctcacgcgca gggtcgcgga aaaactgcaa tgcaaatacg
      901 gtaaacaccc aaaacggcaa agaccaccag ccgcaacata tcgacaccag caggctcaaa
      961 gccaaaccgc cgccaataat cggccagccc tcacgggcga taatcgggtg ggggtaaaga
     1021 cggttcattt gtcatccttt tgttcaagcg ggttaaaatt gccgacatta tagcggaatc
     1081 tgccgtactc cgctggcaaa tgccgtctga acgcggcttc agacggcatc cgagaaagtg
     1141 ctacaatacc ggttttcctt tatttttcag gcggcatcca ttatgtgcaa ccatcatccc
     1201 caacattcgc acgacaacga caccatccgc atccgcggcg cgcgcacgca taatttgaaa
     1261 aacatcgatt tagacattcc gcgccacaaa ctcgtggtgg taacaggatt gtcgggcagc
     1321 ggcaagtcgt cgctggcgtt tgacacgctg tatgccgaag ggcaacggcg ttatgtcgag
     1381 agcctttccg cctatgcgcg gcagtttttg cagatgatgg acaaacccga tgtcgattta
     1441 attgaaggtc tgtcgcctgc aatttccatc gagcaaaaat ccaccagcca caacccacgc
     1501 tccaccgtcg gtacggtaac tgaaatccac gactatctgc gtcttttata cgcccgtgtc
     1561 ggtaccccgt attgtcccga acacaagctg ccgctgtcga gccaaaccgt atcgcagatg
     1621 gtcgatgccg tattgaaact gccggaagac acgcgtgtga tgattcttgc ccccaccgtg
     1681 cgcgagcgta agggcgagtt tgtcgatttc tttgccgact tgcaggcgca gggttttgcg
     1741 cgggtgcgcg tggacggcga ggtctatcag ttggacgaag tgccgaagct ggaaaaaaac
     1801 atcaagcaca atatcgacgt ggtcatcgac cgcgtgaaag tgaaggcgga catcaagcag
     1861 cggctggcgg aaagttttga aaccgcgctg cgccacggca acgagcgtgc gctggcgatg
     1921 gagatggaca gcggcgaaga acattggttt tccgcacgtt tcgcctgccc cgtgtgttca
     1981 tacagcctgc ccgaattaga gccgcgcctg ttttccttca acaacccgat gggttcctgc
     2041 ccgacttgcg acggcttggg caacaccaat ttcttcgacc ccgaaaaagt ggtcgcccac
     2101 cccgaattgt cgctggcttc gggcgcgatt gacggttggg acaagcgcaa ccagttctat
     2161 ttccaaatga ttcagtcgct ggcgcggcat tacggtttcg acgtgcaggc tgcttgggaa
     2221 acgctacctg aaaaagtgaa aaaagtcgtg ctgcacggct caggcaagga agtcattgat
     2281 ttcacctacc tatccgaacg cggcaccacc ttcaaccgca gccacgcctt cgaaggcatc
     2341 atccccaatc tcgaacgccg ctaccgcgaa accgacagcg aaaccgtgcg cgaaaaactg
     2401 cgcgaatacc aaaaccaccg cgcttgcccg agctgcggcg gcgcacgttt gcgcaaagaa
     2461 gcccgctacg tttacgtcgg cggcgagccg ttgcacgaag tttccgcctg gccgctgacc
     2521 aaaacccacc gattctttga aacgctggat ttggacggca acaaaaaaca aatcgccgaa
     2581 aaaatcctca aagaaatcac cgagcggctc ggctttttga ttaacgtcgg gctggattac
     2641 ctgaatctct cccgctccgc cgaaaccctt tccggcggcg aagcccagcg catccgcctt
     2701 gccagccaaa tcggcagcgg cctgaccggc gtgatgtacg ttttggacga accctccatc
     2761 ggcctgcacc agcgcgacaa cgaccgcctg ctcgccaccc tcaaacgcct gcgcgattta
     2821 ggcaacagcg tgattgtggt cgaacacgac gaagacgcca tccgcgaagc cgatttcgtc
     2881 gtcgatatgg gcccgggtgc gggtgagcac ggcggcaacg tactgattgc cgataccccc
     2941 gaaaacgtcg ccaaatgcga aaactccgtt accggacaat acctcagcgg caaaaaatcc
     3001 attgccgtgc cgtctgaacg cacgcccgtc aatcccgacc gaatgctcat cctcaaaggc
     3061 gcgcgcggca acaacctcaa aaacgttacc ctcgaactgc cgctcggttt gattacctgc
     3121 atcaccggcg tatccggcag cggcaagtcc actctgatta acgacaccct cgccaaaatc
     3181 accgcccgcg aactcaaccg cgcccaagaa gaacccgccc catacgacga catccacggt
     3241 ctcgaacacc tcgacaaagt catcaacgtc gaccaatccc ccatcggccg caccccgcgc
     3301 tccaaccccg ccacctacac cggcctgttc acccccatcc gcgaactctt cgccggtgta
     3361 cccctctcgc gcgaacgcgg ctacaacgtc ggcagattct ccttcaacgt caaaggcggc
     3421 cgctgcgaag cctgccaagg cgacggcgtc atcaaagtcg aaatgcactt cctgcccgac
     3481 gtgtacgtcc cctgcgaagt ctgccacggc aaacgctaca accgcgaaac gctcgaaatc
     3541 caatacaaag gcaaaaacat cagccaagtc ctcgacatga ccgtcgaaga agcccgcgaa
     3601 tttttcgacg ccgtccccac cgtatcgcgc aaactgcaaa ccctgatgga cgtaggtctc
     3661 ggctacatcc gcctcggcca atccgccacc accctctccg gcggcgaagc ccagcgcgtc
     3721 aaactcgcct tggaactctc caaacgcgac accggcagaa cgctctacat cctcgacgaa
     3781 cccaccaccg gcctacactt cgccgacatc gccctgctgc tggaagtcat aggccgtctg
     3841 aaaggcaaag gcaactcgat agtgattatc gagcataatc tggatgtgat taaaacagcg
     3901 gattggattg tggacttggg gccggaaggc ggagatggcg gggggaggat tattgctaaa
     3961 ggtagtcctg aacaggtggc gaaagttaag gggagttata ctgggaagta tttgaaggtg
     4021 gttttgaggt aagtttaatt caaaattaaa taggagatta ttaagatgaa accgttaaaa
     4081 acattagaat ttggatttgt ggatgctgca aactacagaa gaagagaaaa taaagattta
     4141 tttaaccgaa tatttgtaaa aggagaatat ttggatgaat tatgtgaacc aaatatttcg
     4201 tttttaatcg gagaaaaggg aactggaaag acagcatatg ctgtttattt aactaataac
     4261 ttctataaaa acatacatgc cactactaag tttgttcgtg aaaccgatta ttcaaaattt
     4321 attcagctaa agaaagcaag acacttaact gtttcagatt ttacaagtat ttggaaagtc
     4381 attttatatc tgttgatatc aaatcaaatc aaatgtaaag aaaacggaat attatcttca
     4441 atatttaata aatttaaagc cttagatgag gctataaatg aatattatta tggcgctttt
     4501 gatccggaaa ttgtacaagc aataacttta atagaaaatt caaaagaagc tgcggaaatg
     4561 atttttggaa aatttgttaa actaggtgaa gaggaatccc aacaaataac ttttacagaa
     4621 agtaaattcc aagcaaattt aggttttatt gaaagaaaat ttaaagatgc tttatctcag
     4681 ttaaagctaa aagataatca tattttgttt attgatggga tagatattag accatcacag
     4741 attccatttg atgaatatca tgagtgtgta aaaggtcttg ctaacgccat atggatgtta
     4801 aataatgata tcttcccttc cattaaagat agtaagggaa ggatgagagt tgtgttattg
     4861 attagacctg atatctttga ttcattaggt ttacaaaatc aaaataccaa acttcaagat
     4921 aattcagtat ttttagactg gaggacggat tataaatctt atagaagttc aaagattttt
     4981 ggcgtttttg atcatctttt gagaacccag caagaaaaac aagatagttt agaaaaaggc
     5041 aactcatggg attattattt tccatggaat gctcctaatt tacatgatga gtataaaaat
     5101 ttaacttcat ttattagctt cctaagaaaa tcgtattatc gacctcgcga tattcttcag
     5161 atgcttactt tgctacaaaa aaataagaaa agtaaggaag attatgtcgt agcagaagat
     5221 tttgataata cttcttttca aagagaatac tcgatatatt tacttggtga aatcaaagat
     5281 catcttttgt tttattatag tcaaagtgat tatcaaaatt tcctgaaatt ttttgaattt
     5341 ttaaacggga aagatagatt taaatatagt gattttttaa aagcatttga acgtttgaaa
     5401 aagcacttac aaacaacatc agtggaaata cctaaattta tgagtactgc taatgagttt
     5461 ttgcaatttt tatttgactt gaatgttatt gcttatttag ataacccaga agatgaaacg
     5521 aaaccatata tccattggtg ctttaaagat agaaattatg caaatatttc tcctaaaata
     5581 aaaactgaaa ctgaatattt aatattttca ggattatcaa aagcccttga tgttggtact
     5641 ccatttaaga acaaacagta acttgtatcc caaccaaccg tgtgcgctac gcacacggtt
     5701 ggtagctgac gtgttgcagt ttcaacagag aaagccgtag cctgcatacc caaccgaccg
     5761 cgtgcgtggc tgtgccacac gccctacctg tgaattacac ggaacttcaa agccaatacg
     5821 agagctttgc aaaattcctt tccctcccga cagccgaaac ccaaacacag gttttcggct
     5881 gttttcgttt caaatatcca ctaattttac cccaataccc ccttaatcct cccaggatac
     5941 ccgataatca ggcatccggg ccgcctttta ggcgcaaaca ggcacactta gcccgagacc
     6001 tttgcaaaat tccccaaaag ggcaaaaata atgtgggtat gttgaggctg cctctttttg
     6061 aaatgacaaa caaaatgccg tctgaaaccc gatttccggt ttcagacggc atttgatgtt
     6121 caatcagttg tcaaacctga ttatgccaaa ccttttgctt tcaacacttc cagcatggta
     6181 gtgcccaact cggcagggct gcgggtgtaa gcgataccgg ctttttcgaa agcggcgaat
     6241 ttttcttccg cagtaccttt gccgccggag ataatcgcac cggcgtgacc catgcgtttg
     6301 cctttaggtg cggtaacacc ggcgatatag ccgacaacag gtttgcttac gttggattgg
     6361 atgtattcgg ctgcttcttc ttccgcagta ccgccaattt caccgatcat gatgatggcg
     6421 tcggtatccg ggtcttcttg gaaaagtttc agtgcgtcga tttggttcat acccggaatc
     6481 gggtcgccgc cgataccgat acaggttgat tgacccaagc ccagtttggt ggtttgtgcc
     6541 acggcttcgt aagtcaatgt accggaacgg gaaatgatgc cgatgcggcc gggagtatgg
     6601 atgtggcccg gcataatgcc gattttgcac tcgcccggag taatcacgcc cgggcagtta
     6661 gggccgacca aacgtgttcc gttaccgttg gtttccaagt agcgtttggc tttgagcatg
     6721 tccaaagtcg gcacgccttc ggtaatcaca acgaccaagc ctacgcctga atcaactgct
     6781 tcaacgatag aatccaacac aaacggagca ggaacgtaaa tcacggatgc atccgcgccg
     6841 gtttctttaa cggcttcttt catggtgttg aacacgggca ggttcaggtg ggtttgaccg
     6901 cctttgcccg gggtaacgcc gccgacaact ttagtgccgt aagccagagc ttgttcggag
     6961 tggaaagtac cgtttttacc ggtgaaacct tgaaccaata ctttagtgtc tttattaatc
     7021 aatacgctca ttcttttctc cttaggcgtt tacggctgca acaatttttt cggctgcgtc
     7081 attcaggccg tctgcagaag tcagtttcag acctgattcg ttcaggattt tcgcgccgag
     7141 ttcggcgttg ttgccttcca aacgaacaac gacaggaacg ttgacgttga tttctttaac
     7201 ggctgccacg atggcttccg caatcatgtc gcaacgtacg ataccgccga agatgttgat
     7261 caatacgcct tgaacggatt tgtcttccag aatcagtttg aacgcttcaa ccacgcggtc
     7321 tttggttgcg ccgccgccaa cgtccaagaa gttggcaggt tggccgcctt tcagtttgat
     7381 gatgtccata gtggccatcg ccaaaccggc accgttcacc atacagccga tgttgccttc
     7441 cagggcaaca tagttcaggt caaattcaga agctttcaac tcgcgttcgt tttcttgaga
     7501 tttgtcgcgc aattcggcga ttttcggcag gcggtagagc gcgttgctgt cgatgccgat
     7561 tttgccgtcc acgcaggcga gcgcgccgtt ttcgcgaact gccagcgggt tgacttcaaa
     7621 cagggcgaag tcattttcga caaacgcttt gtacgcaccg gtcatcagtt tgacgaactc
     7681 gttgatttgt ttgtctttca agcccagttg gaacgcaact tcgcgagctt ggcaaggttg
     7741 caggccgacc agcggatcaa cggttacttt gaagattttt tcaggagttt cggcagcaac
     7801 tttttcgatt tccacgccgc cttcggtaga ggccatgaat gtaatgcggc gggtagaacg
     7861 gtcaaccact gcgcccaagt acagctcggt ttgaaccgga tacatgtctt cgcaaaccaa
     7921 aacactgttg acaggttggc cgttggcatc ggtttggtaa gttaccaagt tggtgccaat
     7981 caggctttca gccacttctt tagcttcttc gcggctttta acgactttta cgccgcccgc
     8041 tttaccgcgg ccgccggcgt gtacttgtgc tttgacaaca gcgaatttgc cgcccaattt
     8101 gtcgtaagct gcagcggctt cttcgccgtt gtgtgccaaa ataccgcctt gtacgggcaa
     8161 accgtagcta gccagcagtt ctttagcctg atactcgtgt aaattcatgg atttctcctt
     8221 tagattagtg gaatgccccg agtctgcctc caaatggcaa gcccgtgcgt ttcagactac
     8281 ctttcggcgg tcggaaaatc gtcgtttcac ttgctgaaag gcaaccacat ccgcgccagc
     8341 agatacatgg ctgcaccgcc tcccccgaga ataaaaaaga cgatgccttt tttacgcgaa
     8401 ttcggacaga gcagataaac ggcaaggctg aaactgataa acaacaatgc cacgcgccaa
     8461 accaattctt tatctttgaa acggttgagc gtataagttt cggtcatcat cacatacatc
     8521 tgccacaatg cggccataca catgctgatg atgcccgccg gtataaagaa aataccgact
     8581 atttctttgt tcataatgaa tgtaaatgct gtttgtttgt ctgacggaat tataaatcag
     8641 aaaacggagc tctcggaaca ttcaatttgt cgaaggcctt aaggccgtct gaaaaatttc
     8701 agacggcctt aatgtctatc aaccgtgcaa agcgcgtttg tcggccgcca atgcagcttc
     8761 gtgaaccact tcggacaagg ttgggtgggc atggataatg cgggcgatgt cttcgctgct
     8821 ggcgaagaat tcgagcgcag tcacgccttc ggtaaccaat tcgctgacaa ccggaccaat
     8881 catgtgtacg cccaagatgc ggtcggtttt ggcatctgcc aacactttaa ccgtaccttt
     8941 ggctttgccc attgccaatg cgcgaccatt cgcaccaaaa cctgaagtac cttttttgta
     9001 ctccacgcct tcggctttga gctgctcttc ggttttaccc acccaagcga tttcaggatc
     9061 ggtgtaaatc acgaacggta cgttgttgaa gtcgatatgc ggtttttgac cggcaatgcg
     9121 ttcggcaacg gcaacgcctt cgtcgctggc tttgtgtgcc aacatcgggc cgcgaaccac
     9181 gtcgccgatt gcccatacgt taggcaggtt ggtacggcat tcgccatcta ctttgataaa
     9241 gccgcgctcg tctttttcca agcctacggc ttccgcgttc aggcctttgg tgtttggaat
     9301 acggccgatg gcaacgatca gtttgtcgaa tacttcggtt ttggcttcgc cagcagcagt
     9361 ttcgtaagca acggaaacac ctttgccttc agacttgatg tcgccgattt tcacgcccaa
     9421 ttcgatgctc agaccttgct ctttggtgaa gtatttgaag gcttctttgg cgatttgttg
     9481 gtcggcggca gccaggaagg tcggcgcggc ttcaagaatg gtaacttccg cacccacgcg
     9541 gttccatacg gaacccattt ccaaaccaat cacaccggaa ccgatcacgc cgagtttggc
     9601 aggtacttcg gtcaggttca atgcgccttc gttgtccaat acgttcacat tgtcgatagc
     9661 gacttgtggc agcggacgcg gtacggaacc ggtcgctacg atgacgtgtt tggcttcgat
     9721 aacggttttc tctcctttgt tatcgacttc gatttggtaa gcatcgccat ttttaccggc
     9781 aaaggaagcc gtaccgaaca gactggttac tttatttttt tggaacagga atttcacgcc
     9841 gccggtcagt ttggtcacga tggcatcttt gcgctcaatc attttggccg cgtcgaattt
     9901 tacgtcaccg acagtgatac cgtgttcggc aaaatcgtgt tgcgcagcgt ggaaatgttc
     9961 gctggattgc aacagggctt tagaagggat acaacctacg ttcaagcaag taccgcccaa
    10021 tgcaggggca ttgcctgctt tgttaacgcc tgcatcgaca caggcagttt tgaaacccag
    10081 ttgtgcggca cggatggcgg caacgtatcc gcccgggcct gcaccaatca ctactacatc
    10141 atattgagac atcgtgtcat cctttgcttt tagcagtatc tcaaaataaa aacgatactt
    10201 ttcgtttctt ttttattttg aatgactgcg ttttgatggt tttaagaaaa agccgtctga
    10261 aagtccatat ctgccgattc attcgaatca aatgtttcag acgacctcaa tcgttcgttg
    10321 agcctattgt agcacgatac tacattacgc tacatctaaa tatagtggat taaatttaaa
    10381 tcaggacaag gcgacgaagc cgcaggcagt acagatagta cggcaaggcg aggcaacgcc
    10441 gtactggttt aaatttaatc cactataaaa attcagacgg ccttttcatt aacaaataaa
    10501 aggccgtctg aaacgattac agatccaaca acaggcgggc cgggtcttcc aacgcgtctt
    10561 taatggctac caaggtcaat acagcttcgc gaccgtcgat gatacggtgg tcgtaagaca
    10621 gagccagata catcattgga cggacaacaa cttgaccgtt ttcaaccaca gcgcgctctt
    10681 tagtggcatg catacccaaa atcgcagatt gaggcgggtt gatgatcggg gtagacatca
    10741 tagaaccaaa agtaccgccg ttggtaatac tgaatgtacc gccggtcaga tcttcgatag
    10801 cgattttgcc gtctttggct tttttcgcgt aatcaacaat tgcttgttcg atgtcggcaa
    10861 tgctcatttg gtcggcatcg cgcaggattg gcacaaccaa accgcgtggg ctgccaatcg
    10921 cgataccgat gtcgaagtag ccgtggtaca cgatgtcttt gccgtcaaca gaagcattca
    10981 caaccgggta ttttttcagg gcggcgacag cggctttaac gaagaaggac ataaagccca
    11041 atttcacgcc gtgttctttc tcgaattttt ctttgtactt cgcacgcaag tccatgattg
    11101 gtttcatgtt gacttcgttg aatgtagtca gaatggcgtt ttcttgttga gaagccaaga
    11161 ggcgttctgc aacacgggca cgcaggcggc tcattggtac gcgttcttca ggacgtgcgc
    11221 cggcaggaag tgcaacggca ggggcaggtt tggctgcggc attttgtacg tcttctttca
    11281 atacgcgacc gtcacggccg gaaccttgca atgcgttcac gtcaacaccg ctctctgcag
    11341 ccagtttggc ggcggcaggc atagcggcgt tgttttgtgc agcagcagga gcggcggccg
    11401 gggcagcttc ggcaggagcg gcggcaggtg cttcagcagc ggcagtagca gcggcaggtg
    11461 cttcagcagc ggcagtagca gcggtatcga tacgtgccaa aacttggtcg gcaacaacgg
    11521 tttcaccgtc ttgcgctacg atttcaacca atacgccggc ttgtggagaa ggtacttcca
    11581 aaaccacttt gtccgtttcg atatcgatca ggatttcgtc acgggcaact gcttcgccaa
    11641 cttttttctt ccattccaag agcgtgcctt cagatacgct ttcagacagc ataggtactt
    11701 ttacatcaat aatcattttg tgtctccaat ggccctttca gacggcctgt tgtgtgtttt
    11761 tcattctgca tatgggtgtt ccgcctgagc agcctcagac ggaatgctac ttggcttaca
    11821 atgccaaagc gtcttcaacc aattgtttca attgagcaat gtgtttgctt gagtagccca
    11881 ctgcaggcga tgcgctgctt ggacgaccag cataagacag tttttgctct tcgctgataa
    11941 cgtcttcgat gcggtggcgg atttggtaga acgcgccttg gtttttcggc tcttcttgcg
    12001 cccaaaccac agattttgcg ttcggatatt tcgccagttc ggctttaacc tcatcgtatg
    12061 ggaacggata cagctgctca acgcggacaa tcgcaacatc atcttccagt ttacgttcgg
    12121 ctcgaccggc ttccaagtca tagtaaacct gaccggcaca caataccacg cgtttcacgc
    12181 tgtcgttgct tgcgcgttcg gcggtatcgc cgataaccgg acggaaagtc gaaccttcgg
    12241 tgaagttttc cagcgggctc attgcccctt tgaatcgcaa caggcgtttg gacatgaaaa
    12301 ttaccagcgg tttgcggtat gaacctaaga cttgacgttg caagaggtgg aacatttgcg
    12361 acgcttcaga tggcatgatg acttgcatat tgttctcaga acacagttgc aaccaacgtt
    12421 ctacgcgtgc agaagagtgc tcagggcctt gaccgtcgta accgtgcggc aggatggtgg
    12481 tcagaccgca caagcgaccc catttggttt cgcctgaaga caggaattga tcaatcgtca
    12541 cttgcgcgcc gttggcgaaa tcgccgaatt gcgcttccca aatggtcagc ttgtcaggtg
    12601 cggagcaggc aaagccgtac tcgaacgcca tcacggcctc ttcgttcaaa atggagtcga
    12661 taaccaggaa ctcgcccatt ccttcgccca tatggcgcag aggaacataa gtaccgtcgt
    12721 cccatttttc gcgtttttga tcgtgcaata cggcgtggcg gtgcgagaac gtaccgcggc
    12781 cggagtcctc accggagata cgcacgccat gacctttggt tacgaggctg gcgtatgcca
    12841 aggtttcggc cataccccaa tctatggcct gtttgccgga tgccatggct ttacgcgctt
    12901 caatcacacg ttttgcagtc ggatgcaggg caaagccttc cggtacggcg gtaaacttct
    12961 cagtgagacg ctcaatatct gcggcaggca aaccggtttc gatgtgttcg cgccaatctt
    13021 tgccttggta tttgctccag tcaatttgtg tgcgttggaa gttgctcaac gttgtttgct
    13081 cgacgtgttc gcctttatcc aaagcatcac ggtaggcttg gatgtaaccg tcagcctcgg
    13141 cttgggttac cacgccttcg gcaatcagtt gctcggtgta caaagcacgc gcacccgggt
    13201 gttgcgatac ttttttgtac atcatcggtt gggtcaaggt cggatcgtcg ccctcgttgt
    13261 ggccccattt acggtagcag acaacgtcaa tcacgatgtc tttatggaat tttttgcggt
    13321 aatccaaagc ggcttggata gcaaagcaaa cacgctcagg atcatcgccg ttcacatgga
    13381 taaccggggc ggaaaccatt tttgcgatat cggtacagtg tacggttgaa cgggtatcgc
    13441 ggatatcgga agtggtaaag ccgatttggt tgttgatgac gatatgaacc gtaccgccgg
    13501 tggtataacc gcgtgtttta gacaggttga atgttgcttg gttgacaccc agaccgataa
    13561 atgcggagtc gccgtgaatc aataccggca agactttgtc gcggccgttt tcgcccaaac
    13621 gtttttgttt ggcgcgcgca gaaccttcca ccaccgggtt gacgatttcc aagtgtgacg
    13681 ggttgaacgc caaagaaaca tgcatcgggc cgtgcggggt agcaatatcg gagctgaagc
    13741 ccatatggta tttcacgtcg ccgctgggca gtttgatttc ggcacgacct tcaaattcgg
    13801 caaacaaatc gccgggtttt ttgcccaaaa tgttcaccaa aacattcaga cggccacggt
    13861 gcgccatacc gatgataact tcttccacac cgtctttacc ggcgttttga atcaggtagt
    13921 tcaaaccggc aatcgcgctt tcgccacctt cgacaccgaa acgtttctga ccgacgtatt
    13981 tggtatgcag ataacgttcc aaagtttcgg cagcggtcat ttctttcaga atacggcgtt
    14041 tttgatcggc attgtagtgc ggcgtggaca atacgctttc aaaataattg cgtacccaac
    14101 ggcgctcttc ggtattggga atatagatat attccaatgc gatgtgaccg cagtaggttt
    14161 gtttgaggtt gctgatgatt tgggataaag gcagtttgcc ccgattggca aaatcgccct
    14221 cgcccatatt gaattggagc gccatatcgg catctgacag accgtggaat ttcggatcga
    14281 gggcttcaat atcgcgcggc gggatacgtt tgagcggatc aagtttggct gcacccacgc
    14341 cttggatacg ataagcagaa atcagccgta aaacgctgac ttgctttttc agcattgcct
    14401 catccgcact gcccgcaacg gcagatgcaa ttttcttttt cgccaaagta acaaatgatt
    14461 cgcgaatcgg tgtgtgtgcg acatcgacag caaccgtccc cggctgtttg ctcaaatcgg
    14521 tgaaatactg cttccatttt tcatcaaccg catcggggtt ttccaaaaaa gcctcgtaca
    14581 attcctcaat gtaaggtgcg tttgaaccga acaggtaaga gaaattgagt ttttcgtcca
    14641 tcatggcgtg cgctcttttc tttaaaatat aaacaaaggg tagaagtccg cgtcctgacg
    14701 gctaaactga tgccgtctga aaagacattt tattctaccc tattttaaac cggattgctt
    14761 gtcttgattt catccgacta caatcagacg gattcgggaa acaatccggt tctctgatat
    14821 tgcctgtttg acaatatatt aatgtatgtt acctctcgcc tggcggcaca taatcgcggc
    14881 gttccgaacc ggtataaagc tggcgcgggc ggccgatttt cagcgaagga tcgctaatca
    14941 tctcgtgcca gtgcgaaatc cagcccacgc tgcgcgacag ggcgaagatg acggtaaaca
    15001 tttcggtcgg gatgcccagc gcggacagga cgatgccgga atagaaatcg acattggggt
    15061 acagtttgcg ttcgataaag aacgggtctt tcagcgcaat ctgttccaat tccatcgcca
    15121 gcttgaattt cggactgtct tccaagccca attccttcaa aacttcatag caggtttcgc
    15181 gcataatgct ggcgcgcgga tccatattgc ggtacacgcg gtgtccgaag cccatcagac
    15241 ggtatttgcg ttgtttcacg ccttccatat attcggcaac acgggacaca tctccgattt
    15301 catccaacat tttcaacacg gcttcattag caccgccatg ggacgcaccc cacaggcagg
    15361 cgataccggc agcgatacag gcaaacgggt tcgcacccga agagcctgcc agacggacgg
    15421 ttgaagttga ggcgttttgc tcgtggtcgg catgcaaaat aaagatgcgg tcgagtgcgc
    15481 gtgccaaaac gggattgggt ttgtagtctt cacacggcgt ggcgaacatc atatgaagga
    15541 agttttcgga ataagaaaga ttattcttcg gataattgaa cggcagaccg tttgaatagc
    15601 ggtagcacat tgccgcaatg gtcgggattt tggaaatcag gcggtaaatc gcgattttgc
    15661 ggtgttcggg attgctaatg tccaagctgt cttggtagaa cgcagacagc gcgccgacca
    15721 cgccgaccat catcgccatc ggatgcgcgt cgcggcggaa cccccggaag aaccaagtca
    15781 gctgttcatg caccatcgtg tggcggcgga cggtattgtc aaattctgcc ttttgctcgg
    15841 gagtcggcag ttcgccgtaa atcaacaggt agcagacttc caaataatcg gacttttcgg
    15901 ccagctgctc gatggggtat ccgcgataat aaagcaagcc ttgatcgccg tcgatgtaag
    15961 taattttaga ctcacagctt gcggttgaaa caaatccggg gtcgaaggaa aacaaacctg
    16021 tattttttgt cagcccccga atgtcaacca catcgtgccc gatgctggct tccaataccg
    16081 gcagctccaa acctgcctga cccggtacgt tgagtttgat tgatttggac atattattgc
    16141 tcctttgcaa gttataaccc acgttactgc atccgcagca cgttctttgc cttcagacgg
    16201 cttttgcatt ttgaaatctg cctgcaggca gatttcagat gcctcatgcc cgtctgattt
    16261 tttcaagcat cgggataagg tgccctttgt ccgtttccga atgcccgtta atcaaggcaa
    16321 gcaattcttg atcttgaaat tcaaggattt cggaaaactc ggacagctct ttatcgctca
    16381 aatgctcgaa ttctttttcc ataaacctgc cgaagattaa atctaactcc aacaatcccc
    16441 ggcgggtttg aaaacggatt ttccgtttgg caatatcgtc aaaaaccatc atttcttaaa
    16501 cggcccgttt caacataatc tctttaatct taccgatggc tcgggtcgga ttcaagtgtt
    16561 tgggacatac gtctacgcag ttcataatgg tgtggcaacg gaacaaacgg tatgggtcgt
    16621 tcagattatc caaacgctca ttagtgatgg tatctcggct gtccgcaatg aagcggtaag
    16681 cattcagcaa accggacgga ccaacgaatt tatcagggtt ccaccaaaat gacgggcagg
    16741 cagtcgaaca gcaggcgcac aaaatacact cgtacaaacc gtccaactct ttacgctctt
    16801 cctgagtttg cagacgctct ttgtccgcat caatcggatt atcgttgaca acataaggtt
    16861 tgacggaatg gtattgtttg aagaactggg tcatatccac aatcaggtcg cggataacag
    16921 gcagacctgg cagaggacga attttaaccg gctgtttcaa gccacgcaga tcggtcaaac
    16981 acgccaagcc gtttttgccg ttgatgttca taccgtcaga accgcaaatc ccttcgcggc
    17041 aggagcggcg gaaagacaag gtatcgtctt gtgctttcag gcgcactaaa gcgtccaaaa
    17101 gtttcacgtc ggtcggttcc aattccaact cgtaacgctg catataaggc ttggcatcaa
    17161 catccgggtt gtaacggtaa atttcaaaac tcattttttc catgagaagt gttccttttc
    17221 tcatcaactg cccaatgatt ttgtattata aattcaagca gttcaatgtg ggtatggtta
    17281 gatttcagac gacctttgag gcgaagaccg tctgaaaacg catcaataaa cgcgcttggc
    17341 cggtttgatg tattccacgc tcaaaggctt ggtgtgcacc ggtttgtagg acaaggtatt
    17401 ggcatctgaa tggtacagcg tatgtttcat ccagttttca tcatcgcgct caggatggtc
    17461 gtctgaagcg tgcgcaccgc gtgattcttt acgtgcttcg gcagacacca aagtcgcttt
    17521 cgccacttca attaggttat ccaattccaa agcctcgata cgcgcggtat tccacacttt
    17581 gctcttgtct ttgatttcgg tacgtttcac acgctcggca atcgccatga cttctcgaac
    17641 gcctttgctc agaatctcat cagtacggaa cacgccggcg tgcaattgta cggagcgttg
    17701 cagttcgcgg cgcaatgcat caacgttttc accatcagtt tgattgtcca aacgctcgat
    17761 acgttggcgg gtcagctcgc ccgcatccgc aggcaaaggt ttccagtcgc tttgctcttt
    17821 gatgaattta atcatgctgt cgccggcagc tttaccgaat accactaagt ccagcaggga
    17881 gttcgtaccc aagcggttcg caccgtgtac ggaagcacag gcgcactcac ctgccgcata
    17941 cagacctttt acaggcactt cgtattcgtc gccttgagga acgacaactt cgccatggta
    18001 gttggtcgga ataccgccca tcatatagtg ggtagtcggc acaacgggaa tcgggtcttt
    18061 aatcggatcg ataccagcga actgaatgga aatctcacgg atgcccggca gtttttccat
    18121 aattttttct gcgccgatat ggtcgatttt cagtaagaca tggtctttgt ttttaccgca
    18181 gccgcgacct tcgtagattt ccatcgccat cgcgcgggaa acaacgtcgc gagaagccaa
    18241 gtcttttacg gtcggcgcat agcgttccat aaagcgttcg ccgtcggcat tcaacagaat
    18301 accgccctcg ccgcgtacgc cttcggtaat caacacgccc gcacctgcca cgccggtcgg
    18361 gtggaattgc cagaattcca tatcttccaa cggaataccc gcacgcgcgc aaatgcccaa
    18421 accatcgccg gtattcatat aggcattggt agaagaagca taaatacgac cgccaccgcc
    18481 ggtagcaaac atcacagctt tagcgtggaa aatataaact tcgccggttt ccatttccat
    18541 ggcggttacg ccgacgacat cgccgttttc atcacgaatc aaatcttgtg ccgtccattc
    18601 cacaaagaat tgcgtattgg cacggacgtt ttgttggtac aaagtatgca gcatcgcatg
    18661 acctgtacgg tcggcaaccg cacaggcgcg ttctaccgcg cgtttaccgt gttcggcagt
    18721 atggccgccg aaaggacgct gataaatttt accgctttcc acacggtcaa aaggcatacc
    18781 catgtgttcc aactcaatta cggcttcagg cgcggcgcgg cacataaact caatcgcatc
    18841 ttggtcgccc aaccagtcgg aacctttcac ggtatcgtac atgtgccagt cccaacggtc
    18901 ttcctgcaca ttacccagag aggcggaaat accgccctgc gccgctacgg tatgcgaacg
    18961 ggtcgggaac actttagaca aaacggcaca attcagaccg gatttggata attggagggc
    19021 tgcgcgtaaa cctgcaccac caccgccgac aatcacggca tcaaacttgc gaacaggaaa
    19081 acccatactt acccccaaat tactttaatt gaatacacca agcagccgac cagccagacg
    19141 atggtggcaa cctgcaaaaa caaacgcacg ccgaagggtt tgatatagtc catccacaaa
    19201 tcgcggatac ccacccaagc gtgcaagaat acggcgatga agctcacttg ggtaaatact
    19261 tttacccaag tttgactaaa aaatgcctgc catgccgaat attctttagg cagggaaaat
    19321 agaaccacta aaagtgcaac ggtataaatc aacataataa ccgcagtcgc acgttgcatc
    19381 gcccaatcgc gcaaaccgta atgggcaccg gtcaatttac gttctaccat aacaacgctc
    19441 ccaaaacgac agtcaaaacc aatgcagaag caaatacggc tttagcggta ttgcgcgcag
    19501 tattcagctc aaggcctttg tgcgcatcca aaaataaaaa gcggataccg gcgagagaat
    19561 ggtgcagata agcccacaat acaccgatta aaaccagctt gaccaaagga tgggaaacaa
    19621 tggcacggta agtttcaaat gcagactctt gactcagggt accggacagg aaatacagca
    19681 ggaaaggcag cataataaac agcccgaccc cgctgatgcg gtgaaggatg gaaactatcc
    19741 cgggtatcgg cagacggatg ttcggcaaat ccagataaac aggacgcggt ttggcagaca
    19801 tagtcaattc ctctgtagtt atatatcgac tcaaaaaccg taaacccatt gctacactgg
    19861 gttacataaa cgttaattta cctgttttat cccattttga acagtgtatt ttaaaaacaa
    19921 actgatagat tttgacaaac acgcgtggaa acagcagatt aaaagaaaga tttttagttt
    19981 tgccgccccc ttcccgacac ggtatcagca tccgacccca tccccaaaaa caaaatgccg
    20041 tctgaaaaaa tttcagacgg cattgttttg ccatatcggt caggctttca atttcgcatc
    20101 ggcatcgcgc aacgcggtac gcagaccttc ctcgataacg ggatggtaga acggcatatc
    20161 cagcatttgc ggaacggtca tcttcatttg atgcgcccat gccagcaagt gcgccaaatg
    20221 ttcggcggca gggcctacga tttccgcgcc gataaagcgg ccggtggctt tttcggcata
    20281 caggcgcata tggcctttgt tcaccagcat cacgcggctg cggccttggt ttttgaacga
    20341 tacttcgccg atgacaaatt cgtcggcttg gtattgcgcg gcaacctgcg cgtatttcag
    20401 accgacaaag ccgatttgcg gactggtaaa caccacgccg atggtgctgc gccgcaaacc
    20461 gccgccgata ttcgggtagc ggcccgcgtt atcgccggca atcttgcctt ggtcggcagc
    20521 ttcatgcagc agaggcagtt ggttggacgc gtcgcccgca ataaagatat gcggaatgct
    20581 ggtctgcatg gtcagtgggt cggcaacggg tacgccgcgc gcgtcttttt cgatattgat
    20641 gttttccaaa ccgatattgt caacgttcgg acggcggccc acggctgcca gcatatattc
    20701 ggcaacaaat acgccttttt cgccgtcctg ctcccaatgg acttctacat tgccgtctgc
    20761 atctagtttg acctcggttt tagcatccag atgcagtttc aattcttcgc cgaacacggc
    20821 tttcgcctcg tctgaaacga cggggtcgga aatgccgccg atgattccgc ccaaaccgaa
    20881 aatttcaact ttcacgccca aacggtgcaa tgcctgaccc agttccaaac cgataacacc
    20941 cggcccgaac acggcaacgc gcttaggcag cgtatcccat gagaaaacgt catcgttgat
    21001 aatcaaacga ttgcccaaag actgccattg cggcaggatg acgggacgcg aaccggtagc
    21061 aatcacgaaa ctttttgccg taatttgagt atgctcgtcg atttggacgg tatgctcgtc
    21121 gataaattta gccgaaccca tgatgcgctt gtcggcaggc cactcttcca catcggcaac
    21181 gacaaagccg acaaaacggt cacgctcgga tttaacgcgc tgcatgacct cttcaccgtt
    21241 gacgacgatg ctgtctttgt ccaaatgcac gccgaacggg tcggtatgca atgcgtggtg
    21301 gcgcgcttct gcggcggcaa tcaagagttt ggaaggcata caacccacgc gcgcgcaggt
    21361 tgtgccgaac acattgtttt caatcaggta aacattatcc gaatgtaaac gggcattgcg
    21421 aaacgcaccc ataccggcag taccgccgcc gattacgacg acatccgctt gaattttttt
    21481 cataatcttt gtccttccgt tttcgtctga aacattgccg acacagaaca tatcggtttc
    21541 agacggcatt taaatatttt cccaatcaaa aacatcattc agataaataa aaacaaaatt
    21601 tcgattgagt gcaacaatat ccaaattctc ttataaatat agagttattc ggatcggcag
    21661 tgtagaaccg cccgaataac tctatccggt atatcagaca gtttaatcaa accgccttaa
    21721 gcaaacagct atcagttttt agccaagtaa gcttccaaat cttcgctgcc gccgatgtat
    21781 ttgccgccga tgaagacttg aggggcagtc atcttgccgg taatggcgcg aacggaagta
    21841 acggttgcat ctttgcccaa tacgatttct tcgtaagaca aacctttgtc ttgcaaagct
    21901 tgtttggctt tggcgcagaa ttggcaacct ggtttagtga aaattgccac agactcttga
    21961 gctttccaat caggagcaac gaatttcagc atagtatctg catcggatac tttgaacgga
    22021 tcgcctggtt cttcaggctc gatgaacatt ttttcaacca cgccgtcgtt aaccagcatg
    22081 gagtaacgcc aagagcgttt accgaagccc aagtcttctt taccgaccag catacccata
    22141 ccttcggtaa attcgccgtt gccatcagga atcatgtaga tgttgtcgga ttcttcttcg
    22201 gcagcccaag cgttcattac gaacgtatcg tttacagata cgcagtagat tgcgtcaacg
    22261 ccgttttctt tgaacgcgcc gaacaattcg ttgtaacgcg gcaggtgtga agaagaacaa
    22321 gtcggggtaa atgcaccggg cagggagaat acgactactt ttttgccttt gaacaaatca
    22381 tcggtagaca catctttcca agtgtcgccg acgcgggtgc ggaatactac ggaaggtact
    22441 ttttgaccgg tacgatcttg caaagccatt ttaagctcct taatagattg gtttgagaaa
    22501 taattgtgaa tcgctgggca ttctacccca tgcaaaatga tttgtataat ttatagttca
    22561 aataatataa attatttaaa actatcaaac acaactccga tttgcctgtt tcccacatat
    22621 aaagccgaat tagaaaaaaa caagctgcaa tgaaaagatt ttccggcaac tgcgcccttg
    22681 cgccgcaaat caggacaaaa taccgtctga aaggctttca gacggtattt attcatacgg
    22741 tctcggctta tttcgccaat tcggcacgca atttgtgggt tacgttcatc atcacttgga
    22801 gttgttccag agtttctttc cagccgcgtg ttttcaggcc gcagtccggg ttaacccaca
    22861 gacgttcaac cggtacaacc tcgatggctt tgcgcaacag gtgctccact tcggcttctg
    22921 tcggtacgcg cgggctgtgg atgtcgtaaa cccccgggcc gatgtcgttc gggtatttga
    22981 attcgccgaa cgcggtcaag agttccatgt cggaacgtga agtctcgatg gtgatcacgt
    23041 ccgcatccat tgcagcaatc gcaggcagga tatcgttgaa ctcggagtag cacatatgag
    23101 tgtggatttg tgtgctgtct tcgcaaccgg cagaggacag gcggaaggat tcgcccgccc
    23161 agttcaggta ggcatcccaa tcggcgcgtt tcagcggcaa gccttcgcgg atggcaggtt
    23221 cgtcaatttg gatgactttg atgcctgctt tttccagatc caatacttcg tcgttcagag
    23281 ccagtgcgat ttgtttgcac acggtagagc gaggaatatc gttgcggaca aaagaccatt
    23341 gcagaatggt tacagggccg gtcaacatac ctttcatcgg gcgtttggtc aggctttgtg
    23401 cgtaagtaga ccaggcgaca gtcatggctt caggacggct tacgtcaccg aagataatcg
    23461 gtggtttaac acaacgtgag ccgtagcttt gtacccagcc gtattgggtg aatgcaaaac
    23521 cgctcaacaa ttcgccgaag tattcgacca tgtcgttacg ctcggcttcg ccgtgtacca
    23581 gtacgtccaa atccagtttt tcttgttctt caaccaccaa ggcgatttct tttttcatcg
    23641 cggcttcgta atcggcggca gacagttcgc cttttttgaa ggctgcgcgt gcttggcgga
    23701 tttcggtagt ttgcgggaaa gaaccgatgt tggtcgtcgg cagcagaggc aggttcaacc
    23761 atgcttgttg cgctttgata cggtcggcaa atggagattt gcgttggtct gcgttggcag
    23821 gcaaatcggc caggcgtttg gcaacgtctg cacggtggat ttcgctgctg ttggcgcgtg
    23881 aatcggcagc agcttggctg gcggccagtt cttcggcaac ggaatcgcga ccttcgttca
    23941 atgcggcttt cagaacgcgc aattcttggg ttttttgcag ggtaaatgcc aaccaagagt
    24001 acaggtccgg tttgttggct ttcagttttt cttcaactga caagtcaaat ggagtgtgca
    24061 gcagagagca agagctggaa atccacaaac ggtcgcccag tttggcttgc agaggctcaa
    24121 cagtttccaa aactttgttc aggttggcgc gccagatgtt gcggccgtcg atgatgcctg
    24181 cagacagaac tttgtcgtag tcggcaaatg cgtccagttg ctcgggagcg cgtaccaagt
    24241 cgatgtgcag gccgtcaaca ggcagggatt tcaacaatgc ggcgtgttcg gcaacagaac
    24301 cgaagtaagt gctcagcagg attttggcac ttactttgct caaagtagcg taaacgtctt
    24361 tgtaggcttc tacccattct ttaggcagat cgacagtcaa agcaggctcg tcgatttgaa
    24421 tccactcggc accggcttca accaaagcag tcaggatttc aacgtaaaca ggcaacagtt
    24481 taggtaacag gctcagacgg tcgaattcaa cggcgccttt ttctttacca acccacagga
    24541 aagtcaacgg acccacaacg gtcggtttgg ctttcaagcc caaagcttgg gcttcttgca
    24601 gttgttggac gtagtgtttg gcgttggctt tgaattcagt attggcgtgg aattcaggca
    24661 ccaagtagtg gtagttggtg tcgaaccatt tggtcatttc gatagcgaat tggtctttgt
    24721 taccgcgcgc cagttggaag aattgttcca aagacaggtt ttggctgtcg aagccgaagc
    24781 gggcgggaat cgcgccggtg gcgacttgca ggtcgaggat gtggtcgtag aaagtgaaat
    24841 cgcctgcggc aacgtaatcg gcgttggcag cagcttggtg tttccagttt ttctcgcgca
    24901 agtctttagc aacagccagc aattcttgct cgctgatttc tttgcgccag tatttttctt
    24961 gggcgaattt caattcgcgg aaggcgccga cacgcgggaa gcctgagaaa tgtaatgttg
    25021 tcatgttgaa ctctccagtt ggattttgta aggaccggtt caatccgaat cggccggtat
    25081 tgtaaatcag aatggctagg atgggggcgg cgttttgctt ttcagacggc ctcggatgcc
    25141 gtctgaagcc gttttcagct ttaagggcgc acgcctaaaa tatggcagat ggcgtaagtc
    25201 agctcgctgc ggttaagcgt atagaagtgg aaatctttca cgccttcgcg ggacaggact
    25261 ttgaccatat cgatggcgat acttgccgcc accagattgc gcgtaccttg gtcgtcgtcc
    25321 aaaccttcat acatttgcga cagccagctt gggattttga cgttggttac ttgagccatt
    25381 ttaccgagct gcttgaagtt ggtaacaggc aaaataccgg ggacgatttc cacatcgata
    25441 cccaacatca cgcagcggtc gcggaagcgc aggtagcgtt ctacgtcaaa gaaaaattgg
    25501 gtgatgacgt ggttcgcgcc cgcatcgatt ttgcgtttca aattaatcag gtcggcttgt
    25561 gcggatttcg cttcgggatg cacttcggga tatgctgcta cagagatgtc gaagtcggcg
    25621 acggagcgta atagcttaac caagtcttcg gcataaaacg gttttttctc gtaaccgggc
    25681 ggctcgtcgc cgcgcagggc gacaatgcgg cggatgccgc tgtcccaata gtctttggcg
    25741 atttggcgca attcgtcggg agaagcgtcg atgccggtca ggtgcggcgc ggcttccaag
    25801 ccggtttctt gcttaatgcg tttgacgatg ccgtgcgtgc ggtcgcgttc gccggaattt
    25861 gcgccgtagg ttacggatac gaacttggga tgcagggttt gcagacggtg gatggaatcc
    25921 cacagcatcg tttccatttg ttcgtttttc ggcgggaaaa attcaaacga aacgttgatg
    25981 tcgcctttca aatcggaaag gctgttgttt aacgcattga tttcttttgc gtaattcatg
    26041 gctgcgtacc ttttattatc ttttatatga tattttcagg caacataata aagttgcgcc
    26101 aaacatgagt caatttcaaa attttcagat ttatcatgaa aatttctaat atattacaaa
    26161 aatagttaaa tttccttgta caagccttct aaatttatga taaagtttgt tatgttataa
    26221 caattcaact aaggcgatat atgaaaccga atattcatcc tgacaattat cgaaccgtcc
    26281 tttttttcga cagcagcgcc aatgaaggct ggttaatccg ctcctgtgcc gaaacacacg
    26341 gaaaaacgat ggtttggaca gacggcaaag aatatccgct gttttcgctg gatacttctt
    26401 ccgcatccca tcccgtctat accggcaagc agcgcaacgt caacaccgaa ggccgtgcca
    26461 gcaaattcaa ccaacgtttc caatccgtca tgtcatcgtt tagaaaggac aaataatgca
    26521 ggttctgtct tcactcaaaa ctgccaaaca acgccaccgc gactgccaaa tcgtcagacg
    26581 caggggcaaa gtttacgtca tctgcaaaag caatccgcgt tttaaagcgc gccaacgctg
    26641 accccctacc ccttcctgct tcgg
//
LOCUS       DAXHNE010000013        26596 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.013, whole genome shotgun sequence.
ACCESSION   DAXHNE010000013 DAXHNE010000000
VERSION     DAXHNE010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26596
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.013"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(67..3165)
                     /locus_tag="ACFM4Z_000401"
     CDS             complement(67..3165)
                     /locus_tag="ACFM4Z_000401"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFC6020501.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLGVLLHYSVFDANNTLLIMRPYQ
                     IAAAERILWKINSSAQAKNWSGPESGGYVWHTTGSGKTLTSFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSESTAGLKRNLEKDDNKIIVTTIQKLNNLMKS
                     EDNLPVYHQQVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFPENALGAETT
                     AGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKKLSAAENRQALL
                     HPERIREITQYILNQFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLH
                     PLKVATIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTNFGTD
                     SKAFQNYYRDLAKRVKNQEVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSR
                     TNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEEYMNGYIDDRTG
                     EVRRGYLDVAKELRERFPDPDKIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALREL
                     QSVDAADEDAMKAFQEKYYLSDEDVQEMRQVPMPSERAVQDYRSAYNDIRDWLRRQKA
                     GEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRA
                     SIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRHEAAGLIAAEALNETA
                     AKRYLISSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGT
                     DI"
     gene            complement(3196..3308)
                     /locus_tag="ACFM4Z_000402"
                     /pseudo
     CDS             complement(3196..3308)
                     /locus_tag="ACFM4Z_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3474..4034)
                     /locus_tag="ACFM4Z_000403"
     CDS             complement(3474..4034)
                     /locus_tag="ACFM4Z_000403"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC6020502.1"
                     /translation="MVWKTLGEIAEYSKNRICSDKLNEHNYVGVDNLLQNREGKKLSG
                     YVPSEGKMTEYIANDILIGNIRPYLKKIWQADCTGGTNGDVLVIRVTDEKVNPKYLYQ
                     VLADDKFFAFNMKHAKGAKMPRGSKAAIMQYKIPIPPLPEQEKIVAILDKFDTLTHSV
                     SEGLPYEIALRRKQYEYYREQLLAFR"
     gene            complement(4060..4811)
                     /locus_tag="ACFM4Z_000404"
                     /pseudo
     CDS             complement(4060..4811)
                     /locus_tag="ACFM4Z_000404"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(4783..4866)
                     /locus_tag="ACFM4Z_000405"
                     /pseudo
     CDS             complement(4783..4866)
                     /locus_tag="ACFM4Z_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-damage-inducible D domain protein"
     gene            complement(4983..6527)
                     /locus_tag="ACFM4Z_000406"
     CDS             complement(4983..6527)
                     /locus_tag="ACFM4Z_000406"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFC6020503.1"
                     /translation="MMTEIQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGNFEDHRIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDGRPFDAIVSNPPYSINWIGSDDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNTDIQFIDASGFFKKETNNNVLTEEHIADIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEIGETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(6600..7604)
                     /gene="rfaD"
                     /locus_tag="ACFM4Z_000407"
     CDS             complement(6600..7604)
                     /gene="rfaD"
                     /locus_tag="ACFM4Z_000407"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFC6020504.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMDNNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VKEELIRYIPFPNALKGKYQSFTQADITKLREAGYKEEFFDVKSGIDRYVKWMLENLA
                     "
     gene            complement(7645..8763)
                     /locus_tag="ACFM4Z_000408"
     CDS             complement(7645..8763)
                     /locus_tag="ACFM4Z_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC6020505.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPFIQKNCFFYKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDISDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEILLRASHI
                     KSYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKNKMRIYPEGREKYLEYHRKFVFQDNIDDFL"
     gene            complement(8765..10000)
                     /locus_tag="ACFM4Z_000409"
     CDS             complement(8765..10000)
                     /locus_tag="ACFM4Z_000409"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6020506.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFSGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGAKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(10037..11008)
                     /gene="hldA"
                     /locus_tag="ACFM4Z_000410"
     CDS             complement(10037..11008)
                     /gene="hldA"
                     /locus_tag="ACFM4Z_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HFC6020507.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(11063..11803)
                     /gene="pyrF"
                     /locus_tag="ACFM4Z_000411"
     CDS             complement(11063..11803)
                     /gene="pyrF"
                     /locus_tag="ACFM4Z_000411"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFC6020508.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(12329..12976)
                     /gene="adk"
                     /locus_tag="ACFM4Z_000412"
     CDS             complement(12329..12976)
                     /gene="adk"
                     /locus_tag="ACFM4Z_000412"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFC6020509.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQAVEAVKAEVLGALGK"
     gene            13192..14031
                     /gene="htpX"
                     /locus_tag="ACFM4Z_000413"
     CDS             13192..14031
                     /gene="htpX"
                     /locus_tag="ACFM4Z_000413"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFC6020510.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGTGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(14332..14892)
                     /locus_tag="ACFM4Z_000414"
     CDS             complement(14332..14892)
                     /locus_tag="ACFM4Z_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020511.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(15006..15398)
                     /locus_tag="ACFM4Z_000415"
     CDS             complement(15006..15398)
                     /locus_tag="ACFM4Z_000415"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6020512.1"
                     /translation="MGKRMTFDTAKSRFQEKFPHLELLEFSGIYKPSSVRCPTHGVVQ
                     LLYYDTAIKSKYGCPECGKLKMKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALN
                     TPYGSIRTKLDGRSNPDNLVCNRYKVLL"
     gene            complement(15606..15998)
                     /locus_tag="ACFM4Z_000416"
     CDS             complement(15606..15998)
                     /locus_tag="ACFM4Z_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6020513.1"
                     /translation="MAKSFNQAASELTDIFPNISLTGFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(16115..16525)
                     /locus_tag="ACFM4Z_000417"
     CDS             complement(16115..16525)
                     /locus_tag="ACFM4Z_000417"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6020514.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(16539..16922)
                     /locus_tag="ACFM4Z_000418"
     CDS             complement(16539..16922)
                     /locus_tag="ACFM4Z_000418"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6020515.1"
                     /translation="MPKSLSLQDVQLRFSSKFPDKTVLKFTKNYEPVTIQCPLHGSVV
                     YGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGEIYEFASTLAASKFIGCSN
                     STLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(17387..18685)
                     /locus_tag="ACFM4Z_000419"
     CDS             complement(17387..18685)
                     /locus_tag="ACFM4Z_000419"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFC6020516.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGVGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMPGWRESTFGVKDYGA
                     LPENAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(18789..19940)
                     /locus_tag="ACFM4Z_000420"
     CDS             complement(18789..19940)
                     /locus_tag="ACFM4Z_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFC6020517.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQSDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGAVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAIERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVVDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            20275..21654
                     /gene="norM"
                     /locus_tag="ACFM4Z_000421"
     CDS             20275..21654
                     /gene="norM"
                     /locus_tag="ACFM4Z_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HFC6020518.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFIDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGTVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            21729..22769
                     /gene="murB"
                     /locus_tag="ACFM4Z_000422"
     CDS             21729..22769
                     /gene="murB"
                     /locus_tag="ACFM4Z_000422"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFC6020519.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLCDIVRLPEFD
                     RDTVLWLGGGSNILLMQDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHT
                     VALGLSGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVELANADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVIGAEKAAALLEQHPDMPHYPQPDGSVKLAA
                     GWLIEQCRLKGFQIGGAAVHDRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            22781..23431
                     /locus_tag="ACFM4Z_000423"
     CDS             22781..23431
                     /locus_tag="ACFM4Z_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFC6020520.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHRKKYDRKIGNGNP"
     gene            23486..24283
                     /locus_tag="ACFM4Z_000424"
     CDS             23486..24283
                     /locus_tag="ACFM4Z_000424"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6020521.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAVKRSLTSDDI
                     NLPIHLDTIDLNQDSAFQSANLARDTSFWRHHANKPVWFCLLPPSSLTHYADTVKQWA
                     ELARACNVQHLIFTSSTSVYGDTARECDEIALPDPQTESARQILAAEQHLLDSGVPNI
                     DILRLGGLYCAERHPVGRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKN
                     IIEPRHPTRREFYTEEAAKLGLPAPDFAPDDSVGKIIRTVCDNGLSL"
     gene            24334..24900
                     /locus_tag="ACFM4Z_000425"
     CDS             24334..24900
                     /locus_tag="ACFM4Z_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032934 - sterol binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFC6020522.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            24920..25810
                     /locus_tag="ACFM4Z_000426"
     CDS             24920..25810
                     /locus_tag="ACFM4Z_000426"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFC6020523.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            25835..26593
                     /locus_tag="ACFM4Z_000427"
     CDS             25835..26593
                     /locus_tag="ACFM4Z_000427"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC6020524.1"
                     /translation="MNPKISSDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NHWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
BASE COUNT         6225 a         6916 c         6428 g         7027 t
ORIGIN      
        1 tccactataa aaaccgcctc tgcataaaat caaaaatgcc gtctgaaagg ttcagacggc
       61 attttgtcaa atatcggtcc ctattccggc aaacttctcc acaaacgccg caatcttttg
      121 aaaaacactt tgtttcttcg tcagatattg cggattgagc ggactcattt tcggcagggt
      181 ttcggtcagt tccgtgccgt tttcgctggc atagccgcgt ttgagcgagc tgattaaata
      241 gcgtttagcg gcggtttcgt tcagggcttc ggcggcaatc aatcctgccg cttcgtgccg
      301 catcacctct tgcgcgtagg tgtaaaaggt ttccagtatg gcgggaacgt cgggcacttt
      361 gtccaaatcc gtatcgttga tgaaatccac aatcagaccc tctttggcgc ggtgtccgat
      421 acttgcgcgg atgatgcggc ggatttcttc caccagctcc gctttgcctt tgattttttt
      481 gtggtgttcg aaaaccagtt gcaaaatgta atccaaattg atttcctgcg atttgagcaa
      541 atccacctca aaaaccacat cgtcccaatc gattttcgat tgctctttct gttcgcctgc
      601 tttttggcgg cgcaaccaat cgcggatgtc gttgtaggcg gaacggtagt cctgcaccgc
      661 cctttcagac ggcatcggca cttgccgcat ttcctgcacg tcttcatcgc tcaagtagta
      721 tttttcttga aacgccttca tcgcatcttc gtccgccgcg tccacactct gcaactcgcg
      781 cagcgcggca aattcatcgt agttctgcaa tacgttttcc gcccgcaggt attcgccgaa
      841 gagtttggca aaatcttttt tgtctttttc cgtttcgatt ttgtcgggat cggggaaacg
      901 ctcgcgcaat tcttttgcca catccaaata accgcgccgc acttcgcccg tccgatcatc
      961 gatatagccg ttcatgtatt cttcgtagct tttttccaac accacgtttt tggtgttttt
     1021 atcgccgaac agggtaatcg catcaatggt tgcctgctcc aaatcgcgga agcagacaat
     1081 attgccgaag gttttggtgg catcgtaaat gcggttggtg cgcgaaaacg cctgcatcag
     1141 gccgtgatag cgcaggtttt tatcgacaaa cagcgtgttc agcgtcggcg cgtcaaaacc
     1201 cgtcaaaaac ataccgacca caatcagcaa atccacttcc tggtttttca cccgttttgc
     1261 caaatcgcgg tagtagttct gaaaggcttt gctgtccgtg ccgaaattgg ttttgaaaca
     1321 ggcgttgtaa tcgttgatgg cagcctgcaa aaattctttc gcgctgctgt ccatcgcttc
     1381 cggttcaaag gtctcatcaa caatttcacc gacggcgttc tgctcttcgt tggccgcaaa
     1441 ggaaaaaatg gtggccactt tcagcgggtg caggctgcct gcctgttgtg ttttgaacgc
     1501 ttcgtaatag cacttcgccg catccacgct gctgaccgca aacatggcgt tgaagccttt
     1561 gccgcccgca ttcaggcggt gcgttttctg cctgaactgg ttcaggatat attgcgtgat
     1621 ttcgcggatg cgttcggggt gcagcagggc ttggcggttt tcggcagcac tcagtttctt
     1681 ctcatcctgt tccgcttcca cggctttgaa ctgcgggcgc acatcgttgt aatccacttt
     1741 gaatttcaat actttttcat cgcggatggc atcggtaatc acataagaat gcaactcacg
     1801 cccgaacacg cccgccgtgg tttccgcgcc caaagcgttt tcgggaaaaa tcggcgtacc
     1861 ggtaaagccg aactggcaga attttttaaa tttctttttc agattttttt gcgcttcgcc
     1921 gaattgcgag cggtggcatt cgtcgaaaat aaagacaacc tgctggtgat aaaccggcag
     1981 attatcttca ctcttcatca ggttgttcag cttttggatg gtggtaacga tgattttgtt
     2041 gtcgtctttt tccaaattgc gtttcaaacc cgccgtgctt tccgaaccgt ttacgctgtc
     2101 gggcgaaaaa cgctggtatt ccttcatcgt ctggtagtcc aaatccttcc tgtccaccac
     2161 gaagaaaacc ttgtcgataa acgccgattc cgtcgccaga cgcgccgcct taaagctggt
     2221 cagcgttttg ccgctgcccg tggtgtgcca aacatagccg ccgctttccg gcccgctcca
     2281 attcttcgcc tgcgccgagc tgttgatttt ccacaaaata cgttcggcgg cggcaatctg
     2341 gtacggccgc ataatcagca gcgtattatt cgcatcgaac acgctgtaat gcagcaaaac
     2401 gcccagcaat acgcttttct gcaggaacgt ggcagtaaag tctttcaaat ccttaatcgg
     2461 atgattgtcc gaccgcgccc aattcatcgt gaaatcgaag ctatttttgt cgcgcttggt
     2521 ggtgttggcg aaatagcgcg tgtccgtgcc gttggaaatc acgaagattt gcaggaattt
     2581 gaacagcgaa ttttcgctgt tgaagctctc tttgctgtaa cggtgcacct gattgaatgc
     2641 ttcgcgcacc gccacaccgc gctttttcaa ttcaatctgc accagcggca gaccgtttac
     2701 caacacggta acgtcataac ggtttgcatg cgtgcccgtc tgctcaaact ggttgataag
     2761 ctgcacatgg ttgcgggcaa ggtttttctt gtccagcaga taaatgtttt tcagacggcc
     2821 gtcatcaaaa gcgaaatcgt aaatatggtc gtcgtggatt ttgcgggttt tatcggtaat
     2881 attttcagac ggtctgtcca gatattccgt caaaaaccgc gcccattcgc cgtctgaaaa
     2941 cgccacatcg ttcagccgct gcaactgcgc gcgcaggttt tccagcagcc tgctttggct
     3001 gttcaaatcc ttgcggtatt cgtacccctg attctgcaaa tcggcgatta actccgcttc
     3061 caaccggttt tccgattggt agctgcccga ctgttcgatt ttttcatatt ggtcgagcac
     3121 gatgaaattc ggcgtttcag cgatgggttt agtttcgagg ttcatatggg tcgtcctttg
     3181 cggtgtcggg ttttatagtg aattaaattt aaaccggtac tgcgttggct cgccttgccg
     3241 tactatttgt actgtctgcg gctcgccgcc ttgtcctgat ttaaatttaa ttcactataa
     3301 acggtttgat gttcggattg ggctgcctga aaatgtgcag gctgctttgg ttggctgcgg
     3361 cggaatgcgg gtcggttgtg tccatcgtag gatgggcttc agcccaccgc cccgcctgcc
     3421 gttgttttac tgttgttgca gcttgctgct gtttcggtgg gctgaagccc accctaccga
     3481 aaggcaagca gctgttcgcg gtaatattcg tattgtttgc ggcgcagggc gatttcgtac
     3541 ggcaggcctt cgctgacgga gtgggtcagg gtgtcgaatt tgtctaggat ggcgacgatt
     3601 ttttcctgtt cggggagtgg tgggatgggg attttatatt gcataatcgc tgctttgctg
     3661 ccacgcggca tttttgcacc tttggcgtgc ttcatattaa aggcaaaaaa tttatcatcg
     3721 gccaatacct gatacagata ttttggatta accttttcat ctgttacccg gataaccaaa
     3781 acatcaccgt ttgttccgcc cgtgcagtct gcctgccaga tttttttcaa atacggacga
     3841 atatttccaa tcaagatgtc attggcaata tattctgtca tttttccctc acttggaaca
     3901 taaccggata actttttacc ttctctattt tgtaagagat tatccacgcc cacgtaatta
     3961 tgttcgttca gtttatccga acaaatacgg ttttttgaat attcggcaat ctcccccaac
     4021 gtcttccaaa ccacatcttt cagacggcat tgatagccat cagctatccc ccccgatttg
     4081 attgtcaaaa tctaaaagaa agtcgcggta ataccggtat tggcgtttgc gcagggctaa
     4141 ttccgcttcc agcgtagctt ccagctcggt gaatttgtca agtattttta caattttttg
     4201 ttgggtttct aggggcggga tgggaataag taagttttgc aattcacttt tagcaagtgc
     4261 tggtatacct gctccgtatt gacattgcat gaatgtaatt tgcttcattt ttaaaaataa
     4321 tatgcaaacc gttgcaatag tttgtcttcg ttcgctttaa tagtatatgc gtgagctcct
     4381 gcaaaaaaag ggatatccat ccaattaaca aaccccgcac ttgctcctcc ttggctaata
     4441 acaatcgtgc cagcttcgtt attgtattca tgccaataac ctgatggatt aataccgcca
     4501 ttgattacgg gatacttatc tttttctcta agtagtgatt tattaagctg ctttcccttt
     4561 cctatacttg ccacttcccc caacggcttc cactccaccg gtgccgtctg aatcatctca
     4621 atcaattttt tcgctttact ttgtatatcc atggttgttt tccgtttctt tcccgctgcg
     4681 gcgggatgtg gggcggttat ccgccgtagg gtgggctcca gcccaacaat ccgcctgctg
     4741 ttttgccgtt gtttcggggg gctgaagccc accctacttt gtctgcaagg tgcacaccga
     4801 ccaatattca ttgcctgttt cgtcgatgtg tttggcgttt tcaaaagcgt tgttttctgt
     4861 tgtcatgtat ttttccccga aaatttcttg atttgaactt gtaaagatta cttacaggtt
     4921 accgcccgta ttttcagacg gcctctttcc cattcagttg ttgcaaaata tgcaacaact
     4981 gcctaggcct caatctctgc aatcacttcg tcaatttcgc gccgcagccg ttcgattttg
     5041 gcgacggttt cgccgatttc ggcgttaagc tgtttgatgt cgataatttc gcgtgtgtct
     5101 tcgggttcaa catagctgct gacggcgagg ttgtagccgt tgtctttgac ggtttgctgg
     5161 gcggcgtttt gggcgatatg cggcacatcg gctttgtcgg cgaagagttt gacgatgtcg
     5221 gcaatgtgtt cttcggttaa gacgttgttg ttggtttctt ttttaaagaa gccgcttgcg
     5281 tcgataaatt ggatgtcggt attgtctttg tgtttggaca aaaccaagat attgacggcg
     5341 atgccggtgc cgtaaaagag attgggcgca agggcgatga cggtttccac gtagttgccc
     5401 tccaccagat attggcggat tttctgctct gcacctccgc gatagaaaat gccggggaat
     5461 gagacgatgg cggcgcggcc tctgccggaa aggtagttca gtgcgtgcag gataaaggca
     5521 aaatcggctt tggatttcgg ggcgagtaca cctgcggggg caaagcggtc gtcgttgatt
     5581 aaggtggggt cgtcgctgcc tatccagttg atggaataag gcggattgga aacgatggca
     5641 tcaaagggcc tgccgtcttt gagcttggga ttggtcagtg tgtcgcccaa ttcgatgtgg
     5701 aatttgttgt aattgacgtt gtgcagaaac atgttcatgc gagcgaggtt gtaggtggtg
     5761 tggttgattt cctgcccgaa gaagccttct tcgatgatgt gctcatcaaa ctgttttttc
     5821 gcctgcaaaa gcagactgcc cgagccgcag gcgggatcgt aaattttatt taccttttcc
     5881 tgcccgtgca ccgccagccg cgcaatcagc ttggagacgc tttgcggggt aaaaaattcg
     5941 ccgccggatt tgcctgcgtt ggcggcgtag ttggaaatca ggtattcgta ggcatcgccg
     6001 aaaaggtcga tgcggtggtc ttcaaaattg ccgaaatcga gttccgccac gcctttgagg
     6061 acggcggcaa ggcgtttgtt tttgtcggca acggtgctgc cgagccggct gctggtggtg
     6121 tcgaagtcgt caaacaggcc tttgatgtct tgttcggacg gatagccgga ggcggagctt
     6181 tcaatcgcgg taaaaatttc tttcagcttg gtgttgagct cttcgttttg atgggcttcg
     6241 gcggcaatat tgcaaaaaag ctggccgggg tagatgaaat aacctttgac tttgacggca
     6301 tcgtctttga tttcgggtgt gatgatgctg tccggcatag cggcgtaatc aatactgctg
     6361 tcgcctgcct gcatatagtc ggtgaagttt tcgctgataa atcggtagaa aagtgtgccg
     6421 agaacgtatt gtttgaagtc ccagccatcc accgcgccgc gtacttcgtc ggcaattttc
     6481 caaatttggc ggtgcagttg ggcgcgttgt tgtatttcgg tcatcatcga aatccatata
     6541 aaagttaaac aaatcaaaat cgcctgatat tttcagacga ttttttacgg gcattcaaat
     6601 taagccaaat tttccagcat ccatttgacg tagcggtcga tgcctgattt gacatcgaaa
     6661 aattcttcct tatatccggc ttcgcgcaat ttggtaatgt cggcttgggt gaagctttgg
     6721 tatttgcctt tgagcgcatt gggaaatggg atgtagcgga taagttcttc ttttaccagc
     6781 tctttcaagc tcatttcagg tttgccttcg gcggcgcggc aggcgttgac ggtggcggcg
     6841 gcgagttcgt taaactgttg gctgcggccg gtaccgaggt tgtagatgcc ggaaagttcg
     6901 ggatggtcga agaagtagag gttgactttg gcaacgtctt cgacgctgac aaagtcgcgg
     6961 gtttgttcgc cgttgccgta gccatcgttg ctgccgaaca ggttgacgta accgtgttcg
     7021 cggtattggt ggaaatggtg gaaggcgacg gatgccatgc ggcctttgtg ttgttcgtgt
     7081 tgtccgtaaa cgttgaagta gcggaagccg acgacttggg cggtcaagcc ttctttcata
     7141 cggcgacgca atacttggtc aaacaggaat ttggagtagc cgtacacgtt gagcggtttt
     7201 tcgagttcgc gctcttcgcg gaagatttcg cctttgccgt aaaccgccgc actggaggca
     7261 taaaggaagg ggatgcgttc gtcctgacac cagtccagca aatccagcgt gtactggtag
     7321 ttgttgtcca tcatatacaa accgtcgtgg ttcatcgtat cggaacacgc gccttgatgg
     7381 aaaacggctt cgatgttttg ataaggtaaa atgtgttccc tcacttggcg gatgaattca
     7441 tgtttatcga gataatgggc gatttcgcac tcggcaaggt ttttgaattt ttcgcctttg
     7501 ctcaaattat cgacggcaac aatgtcagta ataccgcgtt gattaagtgc tttgacgatg
     7561 ttgctgccga taaagccggc cgcgcctgtt acgatgatgg tcatattcgg tttcctttgt
     7621 tttgagtaaa attaataaac ttagttacaa gaaatcatcg atattgtcct gaaacacaaa
     7681 tttgcgatga tattccaaat atttttctct tccttcggga tatattctca tcttattttt
     7741 tgcatttgga ttgatattaa gtttttccca tgtgtaacgg cttagtcgag taccgcaaat
     7801 caaacgacca tcatccaaga aagtaatata accttgatcg aacaaccaat cataagtcgg
     7861 cgttaaagct aacccattta aataatctaa tgcctctttc tcatttttct cagtaataca
     7921 aaccatataa gatttaatat gactagccct taacaaaatc tcatctgtaa ttaatgtaaa
     7981 tggacattgc ggcatatgat ttataacatc cttgcgatat ttctgctgcc cttttctact
     8041 tttctgttta atgatttctt gctctacgaa ttgattaaat tctgaagccg gtatttctaa
     8101 tttttctctt gataagagtt gcgggtgcac gatagagcga aattgataat ccaaaaatat
     8161 tcggaaataa aataatggtt ctgaatcttc tatatctttg acaggcaata gtttaagaat
     8221 tgataaataa ctaattttgg gcaatacaat cttacgccaa attgaccata tatcatcctc
     8281 cgaacggata taacccctac ggtaaccttt agaattttcc aaatggtctg atatatcatg
     8341 gattgaaaag aaaatatttt ctgtaggcag attattaata cttaaaacgt aatcatcata
     8401 aaaccttgaa atatcatgat gatatttctg ttcttgtttg tgatattcaa cttttgcatc
     8461 aaccaaataa cgctgtaaat ttttcttata aaaaaaacaa tttttctgaa tgaaaggata
     8521 ttctaccctc cttccatttt ttgtctcagt aatcccccaa ttttcatact caaaaaatgt
     8581 acttagtttt cgaccactat catcaatata actacctaca tatttgcgtg cttccccatt
     8641 accaccaaaa acagataact tgttattcct gtgaataaaa ctatcttccg cacgtaaatt
     8701 ttgaatacta tcgacaataa aatactctat gttttcaata gtaattcttt gaaaatccgg
     8761 cattttactc tccataaagc gtaatatcca tttgttttaa tatagcgatt aatacatcaa
     8821 caacaatact gtttcccgct tggcgataca tttgagtatc actaactaaa attttaaaat
     8881 catctctaaa acccattagc cgcaaacatt ccctaggtgt taatttacga atacgtccac
     8941 gattatgagt aacataatta tcaaccccgg cacgatgcat tttatgcata gtttgcaata
     9001 atgggcgagc tacaggcaaa tcagtttccg tactggtttt aaaatttttt gttcctcctg
     9061 ccaaaacata atttttgatt ttttcagaaa gataatattt ttcctcaacg ttatttacat
     9121 caaaaataaa gtcatcaaat tcagattcgc gggctgcctg aaaaataaaa tcaccatgcc
     9181 aattaaattg ttgatttgct ttttggcata aggcaatttc tccattaatc tgtgtataac
     9241 gtttttgtct atttttagaa ctggtaacaa atttcgcacc cttttcacgt aaaaaatatt
     9301 tgctatcagt atagtcctcc aaaaaatctt gcatagtatg ttctaattca atcttttcag
     9361 gaaactgaaa accatttata ggaggggtat gaaagccaac aacaaaaata cgctcacgat
     9421 gttgaggaat cccataatcc ttactattca ttatttggaa atataagtca taaccaagtg
     9481 aataaaaaac actttttaca actttccaag tttttccatt atcatgatta agcaagccct
     9541 ttacattttc ataaataaat atctttggtt ggacttcatc cacaacgcga gcaaattcat
     9601 agaataatgt tcctcgtgta tcttctaatc ctgcacgttt gccaaccatg gaaaatgctt
     9661 ggcaaggact gcctccaact aaaatatcaa cttgatttct aaactttctc gcatcaaatt
     9721 gagtaatgtc gttatgccaa aaatcttcat ttaatttata gtttccaaga taactttttt
     9781 taacgtatgg atcaatatct cctgaaaaaa caatggtatg gtttaaattt aatcggtgga
     9841 atgcctgttc aaccgcacca atcccgctga atacggttgc taatctaata tgtgaatcag
     9901 gtttaagaaa agttttagat ttccaacctt gttgactggg aaagagcaaa gttttttgta
     9961 atcgagtatc gtgtgtctgt gccattgtcg aaatagtcat acttatatcg ttctgtttat
    10021 cttatcaata tgaaaactac attgttgatt gccctgacaa tgccttgatc aattcggcaa
    10081 acgagcaaac cgccgtaccg agtttcgcca cgacaacccc ggccgcagta ttggcaaggt
    10141 acatggcttc gggcatggtg cagcctgccg ccaaacccaa gcccattccg gcaatgacgg
    10201 tgtcgcccgc accggatacg tcgtaaactt cttgggcgcg ggtgggctgg taaatcggtt
    10261 cgccttcgct gaacaaggtc atgccttctt cgcttcgggt cagtaaaacg gcggtcaggt
    10321 cgaggtggcg gcgcaggttt tgcgcttttt cggtcagctc gctttcgttt ttccaactgc
    10381 cgaccacttc tttcaattcg gcgcggttag gcgtaatcag agttgcaccg acatattttt
    10441 cgtaatcgtc gcctttgggg tcgattaaga cggttttgcc ggcgtgtttc gcccaatcga
    10501 tcatatcgga gatatgcgac aggccgcctt tgccgtagtc tgaaaaaatg attgcgtcgt
    10561 attcgggcaa gatttcgcgg tatttctgct tgatttgttc caacacttcg cagttgggat
    10621 gttcttcaaa atcaagacgg ataagctgct ggttgcgggc gacgacgcgc agtttgacgg
    10681 tggtggcgat ttgtttgtcg cgcatcagat aggaggcgac gccgtcctgc accatcagcg
    10741 catcgagcgc gtcggcggct tcgtcgttgc cggttacgga caacagccct gccctgccgc
    10801 ccaacgaagc gatgttgcgc gcgacatttg ccgctccgcc cgcgcgttgg tcgattcgtc
    10861 cgattttcgc caccggcacg ggggcttcgg gcgaaatacg ggacacatcg ccgaaccaat
    10921 agcggtcgag catcacgtcg ccgacaacca ggactttggc ttgcgcgaaa cgggatttga
    10981 gggtttcttg ttggaacttg gcggacatat tgcctccgtt gtattttttc agacggcctc
    11041 agcctgtaag gccgtctgaa aatcagtttg cttcaaggtt tgccacgcgg ttcacttcgc
    11101 gcaatacggc taccggatcg gcagcttggg ttacaggacg acccattacc aaataagtcg
    11161 aaccggcagc caaggcttcg gccggtgtca tgatgcggcg ctggtcatca ttattgccgg
    11221 caacgtccaa gcggatgccg ggcgtgacca agacaaaatc ctgtcccaat tcgcggcgca
    11281 gcggcgcggc ttcttgggcg gaacagacca cgccgtccaa gcccgaactt tgcgccagtt
    11341 ttgccaagcg gatgacttgt tcttcagggg cggtgttcaa accgatttcc gccaaatcac
    11401 tttgttccat gctggtcaac acggttacgc cgattaagag cggcttcgtg ccgtatccgg
    11461 caacggcttc tgctgcggct tccatcatac ggcggccgcc cgatgcgtgc atatcgacca
    11521 tccaaacgcc catatcggca gcgactttgc aggcttgcgc gacggtgtgg ggaatatcgt
    11581 ggtatttcaa atcgaggaaa agtttgaaac cttgattgat taagctttct gccaaattgc
    11641 gtcccgtcgc ggtaaacagc tctttgccga ttttgatttg acacaatgtc gggtcaaggt
    11701 tgcggacgaa tccgagcgtg tctttttcgt tggaaaaatc aagggcgacg ataacggggg
    11761 tgcgttgttg cggagtttgg aagtcggaga ttaaaggatt catggtgttt tcgtttcggg
    11821 ttttcagacg acctttatcg tctttttcgc agtatagtgg attaacttta aaccggtacg
    11881 gcgttgtctc gccttagttc aaagagaacg cttctctaag gtgctgaagc acaaagtgaa
    11941 tcggttccgt actatctgta ctgcctgcgg cttcgtcgcc ttgtcctgat ttttgttaat
    12001 ccactataac aacgtaaatt caaaccgtag gtcgggcatt gatgtccgac ctacgcggac
    12061 tcaagcctcc gacaaactgt ttcggataaa tttgaaccgg tacatcagcc gtcattcccg
    12121 cgcaggcggg aatccattga taaaactcag ttcatcagat ttaaacagcg attgcccaaa
    12181 ttcagaaatg gattcccgcc tgcgcgggaa tgacgccaaa tgattatttt tcggttgcaa
    12241 cacgctgttt atcaaaaaca ggtcgtctga aaacctgatt ttctgttttc agagtgcgaa
    12301 gcctgcccgt gggtacgacc ttttcgattt atttgcccaa tgcgcccaat acttcggctt
    12361 tcacggcttc tactgcttgg gtgccgtcaa ctttgatgta tttaggcgcg tgttcgcctt
    12421 ccagtttgct gtaaaaatcg accaaaactt cggtttgctc gtggtaaacg gcaaggcgtt
    12481 ttttcacggt ttcttctttg tcgtcgtcgc gctgaatcaa atcttcgccg gttacgtcgt
    12541 ctttgccttc aactttgggc gggttgtagg taacgtggta agtacggccg gaagccaaat
    12601 gcacgcggcg gccgctcatg cggtcgacaa tcacgctgtc aggcacatcg atttcaacga
    12661 ctgcatccaa atccacgcct gcttcaacca tcgcttcggc ttgtgccaat gtgcgcggga
    12721 aaccgtcaaa caagaaaccg tttttgcagt cgtcttgcgc gatgcgttct ttgaccatgc
    12781 cgataatgat gtcgtcgcgc accaagccgc cttcgtcaat gatttttttc gcttccaaac
    12841 ccaagggcgt gcctgcctta atcgcggcac ggagcatgtc gccggtagag atttgcggaa
    12901 tgccgaacgc tgcggtgatg aattgcgcct gagtgccttt gcccgcgccc ggcgcgccta
    12961 aaagtaatgc tttcatgaag tgatcctttg aaatgtgttt attggaatca aacagatggt
    13021 ttacgattct acacggcttg gcacaatagt tcaaaaacca actgcacaaa ctgccatttg
    13081 caagcaaatg ttttgcatac ctttgccgag tctgatattc acaagccggc aactttttgg
    13141 gacaatcccc caaaatttca aaacatcaca ccctaccaaa aaggaacatc cgtgaaacga
    13201 atttttctgt ttttggctac caatatcgct gttttggtcg taatcaacat tgttttggcg
    13261 gttttgggca tcaacagccg gggcggcacg ggcagcctgt tggcgtattc cgccgtcgtc
    13321 ggcttcactg gttcgattat ttcgctgctg atgtccaaat ttatcgccaa acaatcggtc
    13381 ggcgcggaag ttatcgacac gccgcgcacc gaagaagaag cctggctttt gaacactgtc
    13441 gaagcccaag cgcggcaatg gaacctgaaa acgcccgaag tcgccatcta ccactccccc
    13501 gaacccaatg cctttgccac gggcgcatcg agaaacagct ccctgatcgc cgtcagcacc
    13561 ggtttgctcg accatatgac gcgtgacgaa gtggaagccg tattggcgca cgaaatggca
    13621 cacgtcggca acggcgatat ggttacgctg acgctgattc aaggcgtggt caataccttt
    13681 gtcgtgttcc tgtcgcgcat tattgccaac ctgattgccc gaaacaacga cggcagccag
    13741 tcccagggaa cttatttcct ggtcagcatg gtattccaaa tcctgttcgg cttccttgcc
    13801 agcttaattg tcatgtggtt cagccgacaa cgcgaatacc gcgccgatgc gggcgcggca
    13861 aaactggtcg gcgcgccgaa aatgatttcc gccctgcaaa ggctcaaagg caacccggtc
    13921 gatttgcccg aagaaatgaa cgcaatgggc atcgccggag atacgcgcga ctccctgctc
    13981 agcacccacc cttcgctgga caaccgtatc gcccgcctca aatcgcttta aaccgatttg
    14041 aaacggcaaa aaccgtacgc gcaagcagta cggttttttg tatatgctgt ctgaaaacat
    14101 caggtttcca taccgacacc aaccgtatcc atttccgcac cggatacgcc gcattgatat
    14161 accctgataa accgtatccg gacagaaaat atagtggatt aacaaaaacc agtacggcgt
    14221 tgcctcgcct tagctcaaag aaaacgattc tctaaggtgc tgaagcacca agtgaatcgg
    14281 ttccgtacta tttatactgt ctgcggcttc gtcgccttgt cctgattttt gttaatcccc
    14341 tatatcagat aaaacagcgg taaaattgcc gtctgaaacc aactctgcgc catacgggac
    14401 acgctctgca cgtctatttg cctccgatat atcctcccta ctgtaggaac gtctgccgat
    14461 ttgcacgaaa tcggcacctc tagcaccctt caggatggcg gacgttatcc gttcttggga
    14521 ttctgcaaga cggtcgacgg cattatacag acgctcggtc tgataccctt ccgaagcaac
    14581 ctgcttcagg tattccaaat aaccgtaacc caacgcccct ccgacaagca gggtcgtcaa
    14641 cgctaccagc gttgccgaac ggattttagg gggcatatct tgaagcatat ctctaccgaa
    14701 ccgaaccaaa gtctctttaa agtcggactc tacacgcgta gaaccctcgg aaatcttgaa
    14761 gttcaactca tactgcttcc gtttgtccgc tgtcaaccgc ttgaggttgt catccccgta
    14821 ttggacggtt gacacaaccc tgtacagact gtcctgtatt tcgaccagcg tctgtgcaaa
    14881 gttggcggtc ataactggat tcccaaccct tgccgccgat tctgatactc aaaacggtat
    14941 ccaggcgttc cagcagggct tcgggagaca gcagtgcgta ttcttcaata ttttccatag
    15001 gttgattata gcagaacctt atacctgtta cagacaaggt tgtcgggatt tgaacgtccg
    15061 tcgagcttgg ttcgtataga gccgtagggg gtgtttaggg ctttggcggc agcttgtacg
    15121 ctggggaacg tgagtgtttc gcctgtggcg gtgtcgagga tggagacagg tttttggttt
    15181 tggggaggcg tattttcctt cattttaagt ttcccgcact ccggacaccc atactttgac
    15241 tttatagctg tgtcgtaata caaaagttgg acaaccccgt gcgtaggaca tctaacactg
    15301 gaaggtttat aaatgccact gaactccaac aattctaaat gtggaaattt ctcttgaaag
    15361 cgtgatttgg cggtatcgaa agtcattcgc ttacccataa tttgtctcca taggttatgt
    15421 gcaaatggat tacacaacca aaatatagaa attttcaaat tttttgagac ctacctgaaa
    15481 tcctgcgcaa gcacaatgta aagaaaaatg gtatacgtct ttactaaatc tttacataaa
    15541 ttaactctaa ccgggatttg aacgtccgtc gagcttggtt cgtaggattt agggtgttgt
    15601 gaaccttacc tatcgtaccc aagcactttg tacctgtttg aaataaggtt gtcgggcgac
    15661 gttcgaccct tgagcctgtg attgatttgt tggaaatgta cacctaaagc agcacctgcg
    15721 gcagtaacgc ttgggaacgt gagtgtttcg ttggtcgttg tgtcgaggat gaggaggggc
    15781 ttccctacat ttggaggtgt ttgggttttt gacatcttag cacactcagg acacccgtac
    15841 tttgacttta taagggcatt aaatgtcgaa taacgaacgt tcccgtgcat aggacaatta
    15901 acggttactg ggtaattcac accgtcaaag ccggttagag agatattagg gaatatatca
    15961 gtaagttcag aagctgcttg gttgaaagat ttggccattt gagactccaa aattgattgt
    16021 caaatggatt taatagcaaa tttataaaaa tgtcaaattt ttcgagaagc acctgatagt
    16081 ggcgaggggt atacactgtt aaaaagtgta tacgtcattt agtgcagatg tgaaccttat
    16141 acctccctgc aagtaaagtc tctgtgtgaa cccgaccttt tatacggtca gtgatagctg
    16201 ctgggtttat acccaatgac cttgcagcag atgctcttga ggggaatgta agtgtttcgc
    16261 cagttacagt atcctccaat actgtatttt tagataattt atctattgta tcttctggaa
    16321 ttttgtggga attgacacca taagatgcac attttggaca cccactttta gagtccaaca
    16381 tacttttgaa tgtagagcaa gtaaccctcc cgtgtatagg acagacgatt accgtcgggt
    16441 aacgaacgcc attgaactcc acaagattca aatgtggaaa tttagaggtt agcttagaaa
    16501 ctgcttgagt aaatgtaagt gccatagtgg ttgtgaactt actttaccaa cctataacga
    16561 tacctaatca atcggtcaaa cggagtgcgt ccgcttaaac gtacagctaa agtgctgttg
    16621 gagcaaccga taaatttact ggcagccagt gtacttgcaa actcgtagat ttcccctgtc
    16681 tctgtgtcct ccaacctaat ggcaacggcg tttggagagg gcttttcggg atttcgagta
    16741 cattcaggac atcctgttga ggatttcatg gcagcttgta gattcccgta aacaaccgac
    16801 ccatgcaaag ggcattggat tgttaccggt tcgtagtttt tagtgaactt caaaaccgtc
    16861 ttatcaggaa atttactgga aaaccttaac tgaacatctt gtagcgagag tgattttggc
    16921 attatagacc tttctaaaat gaaagcatta tacaaccaaa atatagaaat tttcaaattt
    16981 tctctgaacg cactggtatc cgatggagct aaatgtaaag aaaagggtat atgtccttac
    17041 taaatattta cacattttaa ctgtatagtg tttgacaaac aacaggcata ggcgcataat
    17101 gcacccgtag ttagatagat agtaaggaga taaccaatga cttagcacct gcccccctct
    17161 ctctcgcgca ccccgcccct cgcttgcggc taatgggctt tgcgcgacat atacactttc
    17221 atctcctgca tggcttaacc gcctatgcaa tagtacacac atcaacaaat caggctggca
    17281 cgtcttggca ttggctgacg taccattgta gcccgaaatg ttgataccct actatttatg
    17341 accaacggcg gattagggct tcagacggca attttactgc aaaaccttat gcgaacggat
    17401 gatgcagcac aatcgtttct tctcggtcgg ggccggtgga gacgatggcg accggcgcgc
    17461 cgcagacttc ttcaatccgt ttcaaatatg ctttggcgtt ttcgggcaat gcgccgtagt
    17521 ccttcacgcc gaaagtggat tcgcgccagc cgggcatggt ttcgtaaatc ggcttgcagg
    17581 tttccaccgc atcggaaccg caaggcagga tgtcggtttt gccgccgtcg ggcaattcat
    17641 agccgacgca gatattgatg gtttcaacgc cgtccattac atcgagttta gtaatacaca
    17701 taccggaaat gccgttgatt tggatggagc gtttcagggc ggcggcatca aaccagccgc
    17761 agcggcgtgc gcgtccggtt accgaaccga attcgtgtcc gcgttctgcc aaacctacgc
    17821 ctacttcgtc gaacaattcg gtcgggaacg ggcccgaacc gacgcgcgtg gtataggctt
    17881 tgacgatgcc caaaacataa tccagcattt gaggacctac gcccgcgcct gccgaagctg
    17941 cgcccgccag acagttggac gaggtaacga agggataagt gccgtagtcg atgtccaaca
    18001 acgcaccttg cgcgccttca aacagcagtt tttcgccgtt tttgtttttc tcgttcaaca
    18061 cgcgggacac gtcggtaatc atcggcgcaa tgcgcggcgc gactttttcg ataaccgcca
    18121 tcacgtcttc cgctttaacc ggctcggcat tgtgcagatg ttgcagttgg acgttgtaat
    18181 aagcaaggac ggcatccagt ttttcacgca gtttttcagg atgcagcaaa tcggcggcgc
    18241 gaatggcgcg gcgtgccact ttgtcttcgt aggcagggcc gatgccgcgg ccggtcgtgc
    18301 cgattttgcc tttgccgcgc gatgcttctc gggcttggtc gagcgcgatg tgataaggca
    18361 ggatcagcgg gcaggtcggc gcgattttca gacggccttc gacgtttttc acgcctgccg
    18421 cgttcaactc gtcgatttcg cccaacaggg cttcggggga gacgacaacg cccgaaccga
    18481 tgaagcagtc cagactttca tgcaggatgc cgctcggaat caggcgcaaa atggtttttt
    18541 tgccgccgac aaccaaggta tgacccgcat tgtggccgcc ttggaagcgc accacgccgc
    18601 cggcttcttc cgccagccag tcaacgattt tacctttacc ctcgtcgccc cactgtgcgc
    18661 cgattactac aacattttta gccatagcca tataacctat cgatattaaa aattattgtg
    18721 ggaatcccca aaccggtgcg gctttgcgcc ccgccacgat ttgcctttca tccgccatga
    18781 acgggtattt agcgtttcac gacctgccaa acgccgtccg tctttttcag acggcctgca
    18841 agctcttccg aaacattgtg tccgataccg tagtcaacca cgacacactg cccttgttca
    18901 cgcaaggctt cgaccgcttc gcgcgccgct tcggcatctt ccgcatcgac caacacggcg
    18961 ggctgccgtt cgatggcggg caaacgcccg ataaagctgc gcaagtcgaa actgaatccc
    19021 gttgccgggc gcgccctgcc gaaatatccg cccaatccgt cataacgccc gccgcgcgcg
    19081 accgcgtcgt ggaaatcggc ggcataggcg gcatacagca agcccgtgtg gtaattgtcg
    19141 acacgcagct cggacaagtc gatatggatt tcacaatcgg ggaatgcgtc gcacaccgcc
    19201 tgcaattcgc ccaacgcgcc gccgaccgcc gacaaatccg gcaaccgtcc gcgcgcgtcg
    19261 gacaacactt cacgcccgcc gtacaggcgc ggcaacagcg agaatgcttt tgcccacatg
    19321 ccgtccagct tccaagcctt gacctgcgct tcgaccgccc cggtatcttt atcctgcatc
    19381 aaggcaagca gcgttgcgga ctgccccgca tccaaatgtg ccgcatcgga caaggcgcga
    19441 aatatgccga tatgccccag cgaaagcagc actttgccca tatcggcaat tttcatgctt
    19501 ttcagcataa ggtctatcag ctcgatgtcg ccacggatgt cggcaaaacc gtacatttcc
    19561 gcccctgcct gcaagggttc gcgcatattc agcagaccgt cggactgcgc gtgcaacacc
    19621 ggaccggcat aacacaaccg attaatccct tggttggcgg ataaaagatg ggcatcgata
    19681 cgcgccacct gcggcgtgat gtcggcgcgt atgcccaact gcctgccgct gagcctgtcc
    19741 gttaccaaaa tggttttcag ggaaagcccc gcatcgatat gcgtcagcag ggaatgtgcg
    19801 tactccatca gcggaggctg taccagttca taaccgtgta cgcggaacag tgccaacaac
    19861 tgctccctcg cgctttcaag ctgccgcgcg ttcgtgggca gtacgtcggc gatatgttcc
    19921 ggaagctgcc atgtctgcat gagaatctgc cttctatttt atcatgtaaa tacaaaaggc
    19981 tgcccgatcc gcaatcagac ggcatctttg ccgcaacgca tcacgccgct gtcaaaacgg
    20041 cgatgcttcc ttccgattca gaatccgaac agcccctgtc cgacggctca atgccgtcaa
    20101 gtcgttaaaa ccaaacttta ccataaaata cacacaatct gaatatactt ttataccctg
    20161 tatccgccgt ctgacggcac ggcatttttt tactatataa tgtgcgccat tatttctcat
    20221 cacttcctgc tgccgttttc tgcttcagac ggcatttttg ttgattgaac acttatgctg
    20281 ctcgacctca accgcttttc cttttccgtc ttcctgaaag aagtccgcct gctgaccgct
    20341 cttgccctgc ccatgctgtt ggcgcaggtc gcgcaggtgg gcatcggttt tatcgatact
    20401 gtgatggcgg gtggtgcagg caaggaagac ttggcggcgg tggctttggg cagcagcgcg
    20461 tttgccacgg tttatattac ctttatgggc attatggcgg cgctgaaccc gatgattgcc
    20521 cagctttacg gcgcgggtaa aaccgacgaa gtgggcgaaa cggggcggca ggggatttgg
    20581 ttcgggctgt ttttgggcgt gttcggcacg gtcttgatgt gggcggcgat tacgccgttc
    20641 cgcaactggc tgaccttgag cgattatgtg gaaggcacaa tggcgcagta tatgctgttc
    20701 accagcttgg cgatgccggc ggcaatggta caccgcgcac tgcacgccta cgcctccagc
    20761 ctgaaccgcc cgcgcctgat tatgttggtc agctttgcgg cgtttgtgtt gaacgtgccg
    20821 ctgaactata ttttcgttta cggcaaattc ggtatgcccg ctttgggcgg cgcaggctgc
    20881 ggcctggcga cgatggcggt gttttggttc agcgcgctgg cattgtggat ttatatcgcc
    20941 aaggaaaatt tcttccgccc attcggactg acggcgaaat tcggcaaacc ggattgggcg
    21001 gtgttcaaac agatttggaa aatcggcgca cccatcgggc tgtcttattt tttggaagcc
    21061 agcgcgtttt cgtttatcgt gtttttgatt gcgcctttcg gcgaggatta tgtggcggcg
    21121 cagcaggtcg gcatcagttt gtcggggatt ctctatatga ttccgcaaag cgtcggctcg
    21181 gcggggacgg tgcgcatcgg cttttcgctt gggcggcgcg aattttcgcg ggcgcgttat
    21241 atttcgggcg tgtcactggt gttaggatgg atgctcgccg tgattaccgt gctttccttg
    21301 gtattattcc gttcgccgct ggtaagtatg tacaacaatg atccggcggt tttaagcatc
    21361 gccgccaccg tcttactgtt cgccggcttg ttccaaccgg cagacttcac ccaatgtatc
    21421 gcctcctacg ccttgcgcgg ctacaaagtt acaaaggtgc cgatgttcat ccacgccgcc
    21481 gccttttggg gctgcggtct gctgccgggc tacctgctcg cctaccgttt cgatatgggc
    21541 atttacggct tctggacggc attgattgcc tcgctcacca tcgccgccat cgccttggtg
    21601 tggtgcttgg aattgtgcag tagggagatg gtcagatcgc ataaggctgt ctgaaaactc
    21661 cgccgattaa aatatgaaac tatgggctga agcccgccac tgacaatacc cattttaaag
    21721 ttcaaaccat gcaacccatc cgataccgaa ccgacctcac cccctacaac accttcggtc
    21781 ttcgcgccca agcccgagcc tttattgcgc ttgaacacgc cgacgggttg tgcgacatcg
    21841 tccgactgcc cgagttcgac cgcgatactg ttttatggct gggcggcggc agcaacatcc
    21901 ttttgatgca ggattacgcc ggactggtcg tacacatgga aaacaaaggc atacgcgaga
    21961 ttgcgcgttc agacggcatg gttctgattg aagcgcaggc gggcgaaatt tggcacgatt
    22021 ttgtcctgca caccgttgcg ctgggtttga gcggtttgga aaacctgagc ctgattccgg
    22081 gtacggtcgg cgcatcgccc gtgcagaaca tcggcgcata cggcgtggag gcgaaagacg
    22141 tgattcacag cgtgcgctgc tttgatttgg atacggagac ctttgtcgag cttgccaatg
    22201 ccgactgccg cttcgcctac cgcgaaagcc tgttcaagca ggagggtaaa gggcgttatg
    22261 tgattgtttc ggtcgtattt gcattaaaaa cgcattttgt gccgaacttg ggttacggcg
    22321 atttggcggc cgccgttgcc gaactgagcg cgggcagggt gccgacggcg aaagatgttt
    22381 ccgatgcagt gtgtgcaatc cgcaacagta aacttcctaa tcctaacgtg cttggtaatg
    22441 tcggcagttt ctttaaaaat cccgtcatcg gcgcggaaaa agctgccgcc ctgttggagc
    22501 agcatcccga tatgccgcat tatccgcagc cggacggttc ggtcaaactc gccgccggct
    22561 ggctaatcga gcaatgtcgt ctgaaaggct tccaaatcgg cggcgcggcg gtacatgaca
    22621 ggcaggcttt ggtcttagtg aacaaaaaca acgcctcggc aaacgatgtc cggcagttgg
    22681 cgcaacacat caaatttaca gtatttgctc ggtttcaggt agaattgcac gccgaaccca
    22741 attggctgcc tgcttcgttc agcctgtaaa tccaaggagt atgcccgtga cccgccccgc
    22801 caaaatcaat acttacacac gcatcatcga cgccagcctt gcgcttttca acgaggaagg
    22861 cgagcgcaac atcagcacca accatattgc cgcccacttg ggcatcagtc cgggcaatct
    22921 ctattaccac ttccgcaaca aagacgaaat catcgtccaa ctgttcaaac gttacagcga
    22981 agccctgctg gaatacctca acgaagccgt gttgccgtct gatgtggaag actccatcaa
    23041 ttatatggcg ggcatttacg acgtgatgtg ggaataccgc ttcctcttta gcgatgtaaa
    23101 caccctgctt gcccgcagtg ccgaattatt gggcgaacac aacaccttca cccaagccaa
    23161 agtctccccg ctcttggtca acctgctgac ccagctcaac ggtctgaaca tcatccaagc
    23221 cgaccaaacc gccatgaacg atctcgccgt caatatgtgg atggtcacga aatactggtt
    23281 cgacttcgac agctccctgc gcggccgcac caagctgacc gaagactcca aagcacgcgg
    23341 catccgccgc accttaagcc tccttcgtcc ttatcttttg cccgaacacc gcaagaaata
    23401 cgaccggaaa atcggcaacg gcaacccgta aacccacagc gtccgaatca gccttcagac
    23461 ggcacttacc cgacggtatc agcgaatgcg cgcagtcccc ccgccccata tctccatcac
    23521 aggcctaggt tacctcggcc tgccgctggc acaaaagttt taccaacacg gcagccgcgt
    23581 tgccgccgtc aaacgcagcc tgacttcgga cgatatcaat ctgcccatac acctcgatac
    23641 catcgacctc aatcaagaca gcgcgtttca aagcgcgaac cttgcccgag atacaagctt
    23701 ttggcggcac catgccaaca aacccgtttg gttctgcctc ttgccgccat catcgctgac
    23761 acattacgcc gataccgtca aacaatgggc agaacttgcc cgggcgtgca acgtgcaaca
    23821 cctgattttc acaagcagta ccagcgttta cggcgataca gcgcgcgaat gcgacgaaat
    23881 cgccctaccc gatccgcaaa ccgagtccgc ccgccaaatc ctcgccgccg aacaacacct
    23941 gctcgacagc ggcgttccga acatcgacat cctgcggctg ggcgggcttt attgcgccga
    24001 acgccatccc gtcggccgcc ttgttcaaaa gcaaaacatc ccgggcggca accgccccat
    24061 caacatcgtc caccgtaata tcgccgtcga aagcctgttt cagacggcat ttaaccccgg
    24121 cggcaggcgg ctgaaaaaca ttatcgaacc gcgccacccg acacgacgcg aattctatac
    24181 ggaagaagcc gccaaactcg gcttgcccgc gccggatttt gcacccgacg acagcgtggg
    24241 caaaatcatc cgtaccgttt gcgataacgg cttaagcctg taaaataagc ggcaacagca
    24301 acaaacaggc attctccgcc ccgatcagaa aatatgtccg ccctcctccc catcatcaac
    24361 cgcctgattc tgcaaagccc ggacagccgc tcggaacttg ccgcctttgc aggcaaaaca
    24421 ctgaccctga acattgccgg gctgaaactg gcgggacgca tcacggaaga cggtttgctc
    24481 tcggcgggaa acggctttgc agacaccgaa attaccttcc gcaacagcgc ggtacagaaa
    24541 atcctccaag gaggcgaacc cggggcgggc gacatcgggc tcgaaggcga cctcatcctc
    24601 ggcatcgcgg tactgtccct gctcggcagc ctgcgttccc gcgcatcgga cgaattggca
    24661 cggattttcg gcacgcaggc agacatcggc agccgtgccg ccgacatcgg acacggcatc
    24721 aaacaaatcg gcaggaacat cgccgaacaa atcggcggat tttcccgcga atccgagtcc
    24781 gcaaacatcg gcaacgaagc ccttgccgac tgcctcgacg aaataagcag actgcgcgac
    24841 ggcgtggaac gcctcaacga acgcctcgac cggctcgaac gcgacatttg gatagactaa
    24901 ccttcagacg gcatccgaca tgaacagccc ttttcacaac atcggcatcg taacccgccc
    24961 caacacgccc gacatccaag acaccgcaca cacgctgatt acctttttga agcagcacgg
    25021 ctttaccgtc tatctcgacg aagtcggcat aaaggaaggc tgcatctata cccaagacac
    25081 cgtcggctgc catatcgtca acaagaccga actggggcaa tactgcgacc tggtcgccgt
    25141 tttaggcgga gacggcacct ttctctccgt cgcccgcgaa atcgccctgc gcgccgttcc
    25201 gattatcggc atcaaccaag ggcatttggg cttcctgacc caaattcccc gcgaatatat
    25261 gacggacaag ctattgcccg ttttagaagg gaaatacctt gccgaagagc gcatcctgat
    25321 tgaggccgca ctcatccgcg aaggcaaaac cgccgaacgc gccatcgccc tcaacgatgc
    25381 cgtcctctcc cgtggcggtg ccggacagat gattgagttt gaagtcttcg tcaatcggga
    25441 attcgtctat acccagcgtt cggacgggct gattgtctcc acccccaccg gatcgaccgc
    25501 ctattcgctt gccgccggcg gccccatcat gcaggcagga ttacacgcct tcacgctcgt
    25561 ccccatctgc ccacaatcca tgaccaaccg ccccatcgcc attccagaca cgtccgaaat
    25621 cgaaatcctc gttacccaag gcggcgacgc gcgcgtccat ttcgacggtc aaacccatat
    25681 cgacgtgcaa aacctcgacc gcatcaccat ccgccgctac cgaaatccct tacgcatttt
    25741 gcacccgacc gactaccaat atttcaaaac cctgcgccaa aaactgcact ggggcgagca
    25801 attagtctaa gccggccttt acccgaaagt atccatgaat cccaaaattt cctccgacca
    25861 tacagaagat gccgtgcgcc tctccaaacg catggcgcaa ctggggcttt gttcacgccg
    25921 cgaagccgac ggctatatcg aacagggttg ggtaacggtc aacggcaaaa ccgccgtact
    25981 cggtcagaaa gtttcaccgg cagaccgtat cgaactgaac aagaaagccc acgaacagca
    26041 ggcggcacgc attaccatcc tgttgaacaa acccgtcggc tatgtcagcg cacaagcgga
    26101 aaaaggctat aaatccgccg ccgaactgat tacccctgaa aatcactggg aaggcgatac
    26161 cggccgcatc cgtttcgatc cgaaacacaa aatcggcctc gcccccgccg gcaggctgga
    26221 catcgactcg gtcggattgc tggtattgac tcaggacggc cgtatcgcca agcagcttat
    26281 cggcgaaaac agcggcagtg aaaaagaata tttggtgcgc gtgcgcggca aattggacga
    26341 aaaaggactt gccttactga atcacggatt gagtttggac ggcgagaaac tgcgtcccgc
    26401 ccaagtagaa tggcaaaacg aagaccaact gcgcttcgtg ttgaaacagg gtaaaaagcg
    26461 gcaaatccgc cgtatgtgcg aactggtcgg actgcgcgtc gtcgggctga aacgcatccg
    26521 catgggcaag gtcaaactcg gcaggctgcc gcccggcaaa tggcgttatc tcgctcccgg
    26581 cgaatcgttt taaata
//
LOCUS       DAXHNE010000014        26198 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.014, whole genome shotgun sequence.
ACCESSION   DAXHNE010000014 DAXHNE010000000
VERSION     DAXHNE010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26198
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.014"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            716..2164
                     /gene="gnd"
                     /locus_tag="ACFM4Z_000428"
     CDS             716..2164
                     /gene="gnd"
                     /locus_tag="ACFM4Z_000428"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HFC6020525.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTGIIGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLDEEDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2227..3498
                     /gene="waaA"
                     /locus_tag="ACFM4Z_000429"
     CDS             2227..3498
                     /gene="waaA"
                     /locus_tag="ACFM4Z_000429"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFC6020526.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMTECRKSGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVRVESADAWREAVEKTLSSEGGGMQMQ
                     AHVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3539..3988
                     /locus_tag="ACFM4Z_000430"
     CDS             3539..3988
                     /locus_tag="ACFM4Z_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HFC6020527.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGVVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            4021..4995
                     /locus_tag="ACFM4Z_000431"
     CDS             4021..4995
                     /locus_tag="ACFM4Z_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFC6020528.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNLGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEKEDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLSFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            5064..5139
                     /locus_tag="ACFM4Z_000432"
     tRNA            5064..5139
                     /locus_tag="ACFM4Z_000432"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5097..5099,aa:Lys,seq:ttt)
     gene            5359..6612
                     /gene="murA"
                     /locus_tag="ACFM4Z_000433"
     CDS             5359..6612
                     /gene="murA"
                     /locus_tag="ACFM4Z_000433"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFC6020529.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGV
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            6693..7871
                     /locus_tag="ACFM4Z_000434"
     CDS             6693..7871
                     /locus_tag="ACFM4Z_000434"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFC6020530.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     QYCMDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKNVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFTADEEAV
                     VKDIADVAEDDMILDIGPKSAAELADLLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGA
                     "
     gene            complement(7938..8186)
                     /locus_tag="ACFM4Z_000435"
     CDS             complement(7938..8186)
                     /locus_tag="ACFM4Z_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC6020531.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(8277..9194)
                     /gene="ftsX"
                     /locus_tag="ACFM4Z_000436"
     CDS             complement(8277..9194)
                     /gene="ftsX"
                     /locus_tag="ACFM4Z_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFC6020532.1"
                     /translation="MSIIHYASLHIESARTALKELLRQPFGTLLTLLMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLARDKRIANVRFIGKEDG
                     LAELQSNLDQNLVSMLDGNPLPDAFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAISLGLCGWLLSTVRPLVDAIFKPYGLNIGWRFFYPEEIA
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            complement(9191..9841)
                     /gene="ftsE"
                     /locus_tag="ACFM4Z_000437"
     CDS             complement(9191..9841)
                     /gene="ftsE"
                     /locus_tag="ACFM4Z_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFC6020533.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(10035..10517)
                     /locus_tag="ACFM4Z_000438"
     CDS             complement(10035..10517)
                     /locus_tag="ACFM4Z_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFC6020534.1"
                     /translation="MKYLNLAAIALAATFAAHTASADELAGWKDGTAQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKTYGNTVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            10641..10994
                     /gene="arsC"
                     /locus_tag="ACFM4Z_000439"
     CDS             10641..10994
                     /gene="arsC"
                     /locus_tag="ACFM4Z_000439"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFC6020535.1"
                     /translation="MPEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPDLDNDALLRAIAEHPALLERPIVLANG
                     KAAVGRPLENIEAIL"
     gene            10991..11647
                     /locus_tag="ACFM4Z_000440"
     CDS             10991..11647
                     /locus_tag="ACFM4Z_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFC6020536.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIDSVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            11763..13157
                     /gene="gltX"
                     /locus_tag="ACFM4Z_000441"
     CDS             11763..13157
                     /gene="gltX"
                     /locus_tag="ACFM4Z_000441"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFC6020537.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLNYDNADNVVYQTRRFDRYKEVIAELLEKGHAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWADL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGATLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITANGKLAELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            13178..13573
                     /locus_tag="ACFM4Z_000442"
     CDS             13178..13573
                     /locus_tag="ACFM4Z_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020538.1"
                     /translation="MPSDVGIRLQTAFKWKLKMKKIFYFLMVVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNKLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGGTKASGN"
     gene            13577..14068
                     /locus_tag="ACFM4Z_000443"
     CDS             13577..14068
                     /locus_tag="ACFM4Z_000443"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6020539.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIR
                     AYYGRCLKDDELPLAFAAVDDFGILLGSATVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDI
                     DKV"
     gene            complement(14192..16786)
                     /gene="mutS"
                     /locus_tag="ACFM4Z_000444"
     CDS             complement(14192..16786)
                     /gene="mutS"
                     /locus_tag="ACFM4Z_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFC6020540.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKNIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSANGSSLLETLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPETHAAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNELEDQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17230..18234
                     /gene="gap"
                     /locus_tag="ACFM4Z_000445"
     CDS             17230..18234
                     /gene="gap"
                     /locus_tag="ACFM4Z_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC6020541.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSILERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(18304..18423)
                     /locus_tag="ACFM4Z_000446"
                     /pseudo
     CDS             complement(18304..18423)
                     /locus_tag="ACFM4Z_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18492..19172)
                     /gene="tsaA"
                     /locus_tag="ACFM4Z_000447"
     CDS             complement(18492..19172)
                     /gene="tsaA"
                     /locus_tag="ACFM4Z_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFC6020542.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLVSAAKACIELNPKFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            19286..19921
                     /locus_tag="ACFM4Z_000448"
     CDS             19286..19921
                     /locus_tag="ACFM4Z_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HFC6020543.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNADEINKYINNQ"
     gene            19958..20926
                     /gene="waaC"
                     /locus_tag="ACFM4Z_000449"
     CDS             19958..20926
                     /gene="waaC"
                     /locus_tag="ACFM4Z_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HFC6020544.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFRAETWREMGHLKQTLRQEVFDFVLDSQGLIKSACF
                     AKMAKSPICGLDKNSAREGWAALAYVKTYAVPKGKNAVWRNRELFAQVFGYVMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            21153..21902
                     /locus_tag="ACFM4Z_000450"
     CDS             21153..21902
                     /locus_tag="ACFM4Z_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFC6020545.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLTPDDLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            21913..22848
                     /locus_tag="ACFM4Z_000451"
     CDS             21913..22848
                     /locus_tag="ACFM4Z_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFC6020546.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKVDLLVAGNGASAVV
                     EFAKQVAGVKKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQVDSEKLVLTFRATAFDAAPVQGGH
                     AEAVNVEATPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEALKN"
     gene            22911..23522
                     /locus_tag="ACFM4Z_000452"
     CDS             22911..23522
                     /locus_tag="ACFM4Z_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFC6020547.1"
                     /translation="MITTYKTITSLTQAEFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     VLERDLQLDCRLTVSLSLVNYEACVAAWKSTRQIEVNTEKPFE"
     gene            complement(23574..23891)
                     /locus_tag="ACFM4Z_000453"
     CDS             complement(23574..23891)
                     /locus_tag="ACFM4Z_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HFC6020548.1"
                     /translation="MQKRIEEIQSKYREWCHLLPQLEEDIRRWKHAVALIRDMDNFYT
                     HEYQACHRAIEDGAEVDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            24049..25320
                     /gene="purD"
                     /locus_tag="ACFM4Z_000454"
     CDS             24049..25320
                     /gene="purD"
                     /locus_tag="ACFM4Z_000454"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFC6020549.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVASGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARIVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPHTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            25354..25956
                     /locus_tag="ACFM4Z_000455"
     CDS             25354..25956
                     /locus_tag="ACFM4Z_000455"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC6020550.1"
                     /translation="MTNVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            25984..>26198
                     /locus_tag="ACFM4Z_000456"
     CDS             25984..>26198
                     /locus_tag="ACFM4Z_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020551.1"
                     /translation="MHTQRKNFSDSISLHDCYCTEIKVEGRNVCFNFEDGFVVPDNNP
                     NNQAGKHLKTDFSRVVLIHKNENAEDD"
BASE COUNT         6274 a         6041 c         7390 g         6493 t
ORIGIN      
        1 gagcggtttc tgttttttcc ggtaaattcc taaaactcaa aatttcatca ttcccacttt
       61 cgtgggaatg acggaatgta ggttcgtggg aatgacgcgg tacaggtttc cgttacgtat
      121 ccccatccga aagtttactt atacacaagc aatccaaagt tatctgactg aaatattgtg
      181 agatttaccc gcaaatctca ttttttccac cacttataca caaatccgga tgcacactct
      241 gccgatatgc agaaataaga cattcgtgcc gacacctgac catttccaca agttccaaac
      301 acgcttccgg cttaaatctc cacagtattc ccgttcttaa aggcgccagc agccattcgg
      361 taaagaaaag taggatgcca tctgcacaac aagcaaacta tccacaaaac ttattcccca
      421 taaaaaacaa tccacaggta tgcacaaaag ggaatccgcc ttaccgacac cctaaaaaaa
      481 ggtaatcctg atgattttta aaataaaaaa aagttaccca cagaaaattt ctcctatcaa
      541 tataatcatc gtttttttat ttaattttta tattttattt ataagccttc agggaaacgc
      601 acggtaaagc gtaaaggcat cttttcaaaa aattaaagag ttaaggattg ttaaacaagt
      661 gtaaatcagg tagagttgcg gtcgataaat taaatctata acggagcttc ctgcaatgaa
      721 aggcgatatt ggcgtaatcg gtttggcggt tatggggcaa aacctgattt tgaatatgaa
      781 cgattgcgga tttaaggttg tcgcctacaa ccgaacaatc ggtaaggtgg acgaattttt
      841 aaacggcgcg gcaaaaggga cgggcattat cggcgcgtat tccctgcaag acttggtcga
      901 taagctggca aaaccgagaa aaatcatgat gatggttcgc gcaggttcgg tcgtcgacga
      961 ctttatcgaa caactgctgc ccctgttgga cgaggaagac atcattatcg acggcggcaa
     1021 tgccaattac cccgacacga cacggcggac acattacctt gccgaaaaag ggattttgtt
     1081 tgtcggggcg ggcgtatccg gcggcgaaga aggcgcgcgg cacggcccgt ccattatgcc
     1141 gggcggggat aagcgcgctt gggaagccgt caaaccgatt tttcaggcga ttgccgccaa
     1201 aacgccgcag ggcgaaccgt gttgcgactg ggtcggcaag gacggcgcgg ggcattttgt
     1261 caaaatggtg cataacggca tcgaatacgg cgatatgcag ttgatttgcg aagcgtacca
     1321 gtttatgaaa gatggtttgg gattgtccta cgatgaaatg caccgcgtgt ttgccgagtg
     1381 gaacaaaacc gagctggatt cttatctgat tgaaatcaca gcggcaattt tgggctataa
     1441 ggacgaaggc ggtgaaccgc tggtcgagaa aatcctcgat acggcagggc aaaaaggcac
     1501 gggcaaatgg acgggcatca atgccttgga tttgggtatt ccgttgacgc tgatttccga
     1561 agcggtattc gcccgctgcg tttcgtcgtt caaagaacag cgcgtgcaga ccggcaagct
     1621 gttcgcccga accgttacgc ccgtcgaagg cggcaaacaa gaatgggttg aggcgttgag
     1681 acaggccctg cttgcatcca aaatcatttc ctacgcacaa ggctttatgc tgatccgcga
     1741 agcgggcgaa agctacggct gggatttgga ctacggcaac actgcgctgc tgtggcgcga
     1801 ggggtgcatc attcgcagcg catttttaag caatatccgc gatgcctatg aaaacaatcc
     1861 cgatttggtg ttcttgggtg cggatccgta tttcaaaaat attttggaaa actgcctgcc
     1921 ggcgtggcgc aaggtggttg ccaaggcggt cgaatgcggc atccctatgc cctgcatggc
     1981 ttcggcgatt acgttcttgg acggctacac gaccgaacgt ctgcctgcca acctcttgca
     2041 ggcacagcgc gactacttcg gcgcgcacac ttacgaacgc accgacaaac cgcgcggcga
     2101 gtttttccat accaactgga cgggcaaggg cggagatacc gcttcgacaa cctacgatat
     2161 ttgattccgt tgccgaaatg ccgtccgaac cgttcggacg gcatttgttt tgcaggaaat
     2221 agaaagatgt tccaatggct ttatgatgta ttgtggctgc ttgcgccgat atggatacgg
     2281 cgttatttgg acaaacgctc cggaagtgcc ccggcatatc gggcgcatcg ggacgagcgt
     2341 ttcggcaagc cgtatccgaa tcccgttacc ggagcggttt ggatacacgc cgtttcggtc
     2401 ggagaaacgc gtgccgccca gcccttgata cgcgagttgc ggcagcgttt tcccgatgcg
     2461 ccgctgctga tgacgcagat gaccccgacg ggacgggaaa ccgcgcaagt tctgtttcct
     2521 gatgcacaat gccgttatct tccgtatgac aaaaaaacgt gggtacggca gtttttgcgc
     2581 gaacaccgcc cgatgttcgg cattttgatg gaaaccgaaa tctggcccaa cctgatgacg
     2641 gagtgccgga aatcgggcgt tccgctgttt ttggcgaatg cgcggctgtc ggaaaaatcg
     2701 ctaaacggtt atctgaaagt ccgccgcctg atccgtcctg ccgccgcttc gctgacgggt
     2761 tgtctggcgc agacggaggc ggatgcggcg cggttggcga aattgggcgc ggcatccgtg
     2821 caggtgtgcg gcaataccaa atacgaccta atgccgtcgg aacagatgaa aacgctggcg
     2881 gggcagtttg aaaaacgcat cggaggcagg ccggttgccg tgtgcggcag cacgcgcgtt
     2941 tatcggggtg aagacgaggc ggaaaaactg ctggcggcgt ggcaacaata tcgcggcgat
     3001 gcgctgctgg tcgtcgtgcc gcgccatccc gagcattttc agacggtatt tgaaacggca
     3061 aaacgcttcg ggtttaaggt tcagcggcgc agcgacggtt tgccggtcga acctgatacg
     3121 caggtgtgga taggcgacag tatgggcgag ctgtatgcgt attacctgtg cgccgatgtc
     3181 gcttttgtcg gcggcagtct ggtcgattcg ggctgtcaga acattatcga accgctttcc
     3241 tgcggcgttc cgacgatatt cggcttttca acctacaatt tttccgaagc ctgccggcac
     3301 gccttggcat cgggggcggc ggttcgggtt gagtcggcgg acgcgtggcg ggaagccgtt
     3361 gaaaaaacct tatcgtccga ggggggcgga atgcagatgc aggcgcacgt ggacggcttt
     3421 atcgcacaac atcgcggagc gggcgcgaga atcgccgagg cggtacggga agcggtatgc
     3481 ggacatcggg gacgatagtg atacaatccg tatccgagtt ttccggttgg agcatcgtat
     3541 gagtatttat gccgtcgcgc acatcgtcca cctgtattgc gccattgcct ttgtcggcgg
     3601 cgtgtttttt gaagtgctgg ttttgtccgt cctgcatacg ggacgggtgt cgtgcgaggc
     3661 gcggcgcgaa gtggaaaagg caatgtctta ccgcgccgtc agggtgatgc cgtttgtggt
     3721 cggactgctg ttcgccagcg gcgtcgtgat ggcggcaaac cgctatcttt ctatattggg
     3781 cgaaccgttt gccacttcct tcggtacgat gctgacgctg aaaatcctgt tggcgttcag
     3841 cgtgttggcg cacttcgcca tcgccgtcgt caaaatggcg cgttccacac tgaccgtcgg
     3901 ctggtcgaaa tacatacacg ccgtcgtctt tacccatatg ctgctgattg tctttttggc
     3961 aaaagcgatg ttttatatca gctggtaaca caattacatt tcaaatcagg agattgaacc
     4021 atgacagaat atcccggcat cgggtcgccg ttgttttacg gcgttttttt tgcggcagtg
     4081 ctggtcatga ttgccttgga tatgttttcg ctgaagaaaa acggcagcca taaagtcggc
     4141 gtcaaagaag ccttggcatg gagcggcttg tgggttgccg tatcctgcct gttcgcgggc
     4201 tggctgtatt tcgaactcgc cggcaatctc ggctacggcg cggcagtcgc aaaagaaaaa
     4261 gtattggaat tctttaccgg ctacattttg gaaaaatcgt tggcagtcga caatattttc
     4321 gtgttcctga tgatattcgg ctacttcaaa gtcgcaccac agtttcagca ccgcgtgctg
     4381 ctgtacggcg tattgggcgc attggtattg cgcactgtca tgattttcgt cggcgcggca
     4441 ctggttcagc agtttgagtg gattctgtat ctgtttggcg cgttcctgct ctatactggt
     4501 atacacatga tgaagcccga aggcgatgag aaagaggatt tggcaaacag caggctgctg
     4561 aatgccgtca agaaagtcgt tccggtcggc acggaatttc acggcgagaa attttttacc
     4621 gtcgaaaacg gcaaaaaaat cgccacgccg ctgtttttgg tgctggtcat gattgaattg
     4681 agcgatgtcg tgtttgccgt ggacagtatt cccgccgtct ttgctgttac caccgatccg
     4741 tttatcgtgc tgacctccaa tattttcgct attttgggtt tgcgggcgat gtatttcctg
     4801 ctggcggatg tggcggaacg ctttatcttc ctgaaatacg gcttggcatt cgtgttgagt
     4861 tttatcggtg tgaaaatgct ggtaatgcat tgggtgcata tcccgatttc cgtttcgctg
     4921 tcggtcgtgt tcggcgcgtt gggtgcatcg atactgacct cgttaattta tacgaaaaaa
     4981 cagcctgata aataaaatgc cgtctcaacg ccgacaaaat ggttgacagc atcaggcttg
     5041 atgtatagaa tttcgagctt gtcgggtcgt tagctcagcc ggtagagcag cggactttta
     5101 atccgttggt cgagcgttcg aatcgctcac gacccaccag ataaaacaaa aaaagctgag
     5161 ttttcaaaac tcggcttttt ttgttttccg acaatccttg ttcggaaagc gcggcatttc
     5221 gtcaaattct ttggtgcggc ttccgtattt ttcccgaaaa tcccttataa ttccgcctct
     5281 gcaaccttca tcgggtgatg ctttatgcgt tgtcccgaga attgaaatat tacaagtacc
     5341 caccaggaaa gaacaaacgt ggacaaactg aaaatctccg caaacggccc gctcaacggg
     5401 gaaataacgg tctcgggcgc gaaaaacgcg gcattgccgc tgatgtgcgc gggtttgctg
     5461 acatcgggta cgttgcgcct gaaaaacgtc cctatgctgg cagatgtcaa aaccacgcaa
     5521 aaactgcttc aggggatggg cgcgcgcgtc ctgaccgaca atatcagcga atttgaaatc
     5581 aacggcggta cggtcaacaa cacgtgcgcc ccttacgagt tggttcggac gatgcgcgct
     5641 tcgattttgg tgctgggccc gacgctggcg cgtttcggcg aggcgcaagt cagcctgccg
     5701 ggcggctgcg ccatcggttc gcgccccgtc gatcagcatt tgaaaggctt ggaagcgatg
     5761 ggggcggaaa tcgtcatcga acacggttac gtcaaggcta agggtaaact caaaggtacg
     5821 cgcgtggcga tggatgtcgt taccgtcggc ggcacggaaa acctgctgat ggcggcgacg
     5881 ctggcggaag gtacgacggt tttggaaaac tgcgccatcg agcctgaagt ggtcgatttg
     5941 gcggaatgcc tggttaaaat gggcgcgaaa atcagcggca tcggaacatc gaccatgatc
     6001 gtggaaggtg tggacgagct gcacggctgc gaacacagcg tcgtccccga ccggatcgag
     6061 gcggggacgt tcctgtgcgc ggtggcgata accggcggca gggtggtttt gcggaatgcc
     6121 gccccgaaaa cgatggaagt ggtgttggac aaactggttg aggcaggtgc ggtgattgag
     6181 gcgggcgacg attggattgc catcgatatg cggcagcgtc cgaaggcggt ggacatccgc
     6241 acggtcgtcc accccggctt ccccacggat atgcaggcgc agtttatggc attgaatgcc
     6301 gtggcagagg gaagctgccg cgtggtggaa acgatttttg aaaaccgctt tatgcacgtc
     6361 cccgagttga accggatggg ggcgaacatc acaaccgagg gcaatacggc atttgtgcag
     6421 ggtgtggaac ggctttccgg cgcagtcgtc aaggcgacgg atttgcgtgc gtccgcaagc
     6481 ctcgttatcg ccggtttggc ggcgcgcggc gaaaccgtgg tcgaacagat ttaccacttg
     6541 gatcgcggtt atgaaaatat tgaaaaaaaa ctcggcagcg tcggcgcgaa aatcgagcgc
     6601 gtatccggct gatcgggttg cgatgccgtc tgaagccttc agacggcatc aattgtttct
     6661 gtatcgcaaa cactgattga aaaggactga ttatggcatt tttaaaactg accgaacaaa
     6721 acgtgcaggg caaaaccgtc ctcatccgcg ccgatatgaa cgtgccgttc aaagacggca
     6781 aaatcagcga cgacacccgt atccgcgcct cgctcgcgtc catccaatac tgcatggaca
     6841 acggcgcatc cgttatcgtg atgacccact tgggccgccc gaccgaaggc gagttccacc
     6901 ccgaagacga cgttgcgccc gttgccgcgc atttgggcgg cctgttgggc aaaaacgtga
     6961 aagtattgaa cgactggcgc gaaaacaaac cggctttgaa cgcgggcgat gtcgtgatgc
     7021 tgcaaaacgt gcgtatcaac aaaggcgaga agaaaaacga tttggaactg ggcaaagcct
     7081 acgcttcttt gtgcgacgtg ttcgtcaatg acgcgttcgg caccgcgcac cgcgcccaag
     7141 cctcgaccga agccgtcgcc caagccgctc ccgttgcctg cgccggcgta ttgatggcgg
     7201 gcgaactcga cgctttgggc aaagccttga aacagcccgc gcgcccgatg gttgccatcg
     7261 ttgccggcag caaagtgtcc accaagctga ccatcctcga atcgctggcg gacaaagtcg
     7321 accaactcat cgtcggcggc ggcattgcca acaccttcct gttggcggaa ggcaaagcca
     7381 tcggcaaatc tttggcggaa cacgatttgg tggaagaatc caaaaaaatt atggcaaaaa
     7441 tggcggccaa aggcggttct gtgccgctgc cgaccgatgt cgtcgttgcc aaagccttta
     7501 ccgctgatga ggaagcggtc gtgaaagaca ttgccgacgt tgccgaagac gatatgattt
     7561 tggacatcgg cccgaaatcc gccgccgagc ttgccgattt gctcaaagct gccggtacgg
     7621 ttgtatggaa cggccctgtc ggcgtgtttg agttcgatca attcgcaggc ggcacgaaag
     7681 ctcttgccga agccattgcc caaagcaaag cattctcgat tgcaggcggt ggcgacacgc
     7741 tggcggcgat tgctaaattc ggcgttaccg accaaatcgg ctatatttct accggcggag
     7801 gcgcgtttct tgagttcttg gaaggtaaag agttgcctgc cgtagccgct ttggaaaaac
     7861 gcggcgcgta agcggtttga tataaaaaat gccgtctgaa acgttgtgct gtttctgacg
     7921 gcatttttgt gtggcgatta ttgtgctttg gctgcccatt ccgccggagt ggcggcaacc
     7981 gaaatggaaa gtgcgtgcag ttcgttactt tccagttggg ctttgagtcc gtctttaatc
     8041 aggcggtggc gcgcgaggcg tgccttgcct tcaaattctg atgaaacgat gacggcgaaa
     8101 aaatggtgtc cgtcgccttc tacttcgata tgttcgcatt ttgccacgcc ttcaatcatg
     8161 gcttttactt gttccgaggt cagcatggta ggttccttgt tggaaaaatg cgccattata
     8221 ctggcaaatt gaaatgccgt ctgaaaacgg gttcggacgg catttttgac ggtgttttat
     8281 tttttggctt tgaagccgag caggtgctgg gtggtggcaa gccacgcgcc gaatacgccc
     8341 aacgcgatga cgaagccgaa caccagcgcg atttcttcag gataaaagaa acgccagccg
     8401 atattgagcc cgtagggttt gaagatggca tcgaccaagg ggcgcactgt ggaaagcagc
     8461 caaccgcaaa gccccaggct gatggcggcg gaaaggatgc tctgccacat tgcctgataa
     8521 aggaaggggc ggcggatgaa cgatgcgggc gcgcccaaga gtttggtgat ttcgatttct
     8581 tctttgcggc tgaggatttg caggcggatg gtgttgtgtg cgacaaggac gaacgccata
     8641 cccagcgtca gggaaagaaa ccataaaatt ttacggataa attcgttgat ttggtacagc
     8701 gtttgcaccc attcggtatc catagacgcg gattcgacca taggcagttt ggtaatgtcc
     8761 cggtagattg cctgcatttg cgcgggcgtt gtcgcggggt cgggggtaac gataaatgcg
     8821 tcgggcagcg ggttgccgtc gagcatcgaa accaaatttt ggtcgaggtt ggactgtaat
     8881 tccgccaagc cgtcttcctt gccgatgaag cggacgttgg cgatccgttt gtcgcgcgcc
     8941 agcaggctgc ggacggtatc gctgtcgctt tgtgcggcgg cggtttccat atagacggtg
     9001 atttgcggcg actcgttgag tttgcccaac acgctttgcc cgctttgaat gcccaaatac
     9061 ataaacagcg gcagggtcat cgcgacggcg agcatcagga gggtgagcag cgtgccgaag
     9121 ggttggcgca gaagctcttt gagcgcggtg cgggcggatt cgatgtgcag cgaggcgtag
     9181 tggatgatgc tcatgcgagt cgtcctttcg agaggcgcag gatgcggtgt ccgtagtccg
     9241 ccatcagggt ttcgtcatgg gcggcaacga tgacggttgt tcccgcttcg tggaaggttt
     9301 tgaacaattc catgatgtcg agcgcgtagg cgcggtcgag gttggcggag ggttcgtcgg
     9361 caatcagcag gccgggctgg tgaacgacgg cgcgggcgat gcacaggcgt tgttgttcac
     9421 cgccggagag ggttacggga tcgtccaatt ctcgtccttt caggccgact ttttcgatgg
     9481 cgatgcgggc gcgttcttcg gctttgcgcg gcggatagcc gataatccga agcggcagga
     9541 tgacgttttg caggacgttg cggtcgtaga ggattttgtg gtcttggaac acgatgccga
     9601 tgtgttggcg cataaagccg atttggttgt cggacaatgt gccgaggtcc tgcccgttaa
     9661 acaggatttt gcccctgctc ggcttggtga tgcccgaaat cagcttgagg acggtggatt
     9721 tgcccgaacc ggagtgtccc gcgataaaaa tcatctcgcc tttgttgatt tggaagctga
     9781 cgtttttcag ggcttcaaaa ccgccgggat aggttttgga aacttgttcg aaacggatca
     9841 taggaggtcc tgtaaggaag ggcggcatat cgtttcgggc ggttgcgtcc gcagggatat
     9901 gccgtctgaa caataggtaa gacagtgccg attataaaga ttccgcctgc ttttagatag
     9961 tggataagtt tggcggatat gaaggtcggg cgggaaaaat gccgtctgaa gcggcttcag
    10021 acggcatccg gcgtttaacg gcattttgaa tgggcgagtt tgacggcttc ggtcaggctt
    10081 ttttcgttca cttccccggt aatggtctgc ctgtatccgc atttcggtgc ttcgacgacg
    10141 gtaaagggca gtacgccgac agtgtttccg taggttttca taaagtttcg gctgttcgcc
    10201 ccggtgtaac gccaaatcgg gtaggaaaca ggagtttgtt tgaggaagtt gccgatattg
    10261 tcggatgtgt cgagcgcgat gccgaccata tcgacgctgc cttttttctg cgctttgtac
    10321 catttggaca tggcgggcat ctcttttcgg cacgggccgc accaagtcgc ccacaggttg
    10381 acgatgcgta cgggggcttt gagcgattgc aggctttgcg cggtaccgtc tttccatccg
    10441 gcaagttcgt ctgccgaggc ggtatgtgcg gcaaatgtgg cggcaagggc gattgcggca
    10501 agattcaagt atttcatcgt tttgtccttt gtatgttgat tcgggtattg taaaccgttt
    10561 ggtacggttg ggggcgggat attgagtaaa accctgcgta agccgataat aaggggattg
    10621 ttgagaagca aggaaatgtc atgcctgaaa tcaaaatttt ccacaatccg cgttgcagca
    10681 aatcgcgtgc cgccttgtcc ctattggaag aacgcggcat tgctgccgaa gtggtcaaat
    10741 atttggatac gccgcccgac ttgtccgaat tgaaggatat tttcaacaaa ttgggcttgg
    10801 catcggcgcg cgggatgatg cgtgtgaaag atgatttgta caaggaattg ggtttggaca
    10861 accccgattt ggacaatgac gcgctgctgc gtgccatcgc cgagcatccc gccctgttgg
    10921 agcgtccgat tgttttggcg aacggtaagg cggctgtcgg caggcctctg gaaaatatcg
    10981 aagcgatatt gtgaacaaac ggctgttttg cagccgcaac ggcttgcgct attacctgtt
    11041 gggcggtttc tgtttgagtg tttttccgct tttgctggtg tttgcctcat ccgtttgggc
    11101 ggtttatcgg acgggcgggc aggttttgcc gccgtatgtc cgggcggatg ccgcgctggt
    11161 gttgggcgcc gccgcgtggg acaaacgccc ttccccggtt ttccgcgaac gcatcaacca
    11221 cgccatcgcg ctttatcaga gccgcagggt aggcaaaatc gtgtttacgg gagggcgaac
    11281 caaaaaaggc tatatgaccg aagccgaggt cgggcggcgg tacgcgctca aacagggggt
    11341 tccggcgcgc aacatcctgt ttgaaaacac ttcgcgcaac acttatgaaa acctgaacaa
    11401 catccgtccg gttttgcgtg ccaacggtat tgacagcgtg gtgattgtca gcgatcccta
    11461 ccatttggcg cgggcggcgg aaatggcgga ggatttgggt gtccgcgttt atatgtctgc
    11521 caccccgacc acgcggtttg atgcgggaaa taagaaaaaa atctttatgc tgcaagaggg
    11581 atatgcgtta tccctctacc gtttggaaaa atggggcagc cgctttttgg gatggctgtc
    11641 tgattgaagc ggtagaaaat gccgtctgaa acttatccgc aggcagggtt cggacggcat
    11701 ttttgatgcc caagcacggt aaaaccagta aaataaacgc tttcaaagat ttcggagcca
    11761 tcatgaccgt caaaacccgt ttcgccccca gccccaccgg ctacctgcac attggcggcg
    11821 ttcgcaccgc cttgttttcg tgggcgtttg cccgccatca taaaggcgaa tttttattgc
    11881 gcatcgaaga caccgacttg gcgcgttcta ccgccgaatc cgtcaacatc atcctcgacg
    11941 gcatgaaatg ggtcggcctg aactacgaca acgccgacaa tgtcgtgtac caaacccgcc
    12001 gtttcgaccg ctacaaagaa gtcattgccg aacttttaga aaaaggccat gcctactact
    12061 gctattgcag caaagaagag ctggaagcca tgcgcgagaa agccgaaaaa gaaggcacgg
    12121 cgacttacga ccgccgctgg cggccggaag cgggcaaaac cctgcccgaa atccctgccg
    12181 gcgtgcaacc cgtcgtccgc ttcaaaacgc ctttggatgg cgtaaccaag tgggcggact
    12241 tggtcaaagg cgaaatttcc atccccaacg aagccctcga cgacctgatt atcgcacgcg
    12301 ccgacggcac accgacctac aacttctgcg tcgtggtgga cgactacgac atgggcgtta
    12361 cccacgttat ccggggcgac gaccatgtga acaacacccc gaaacaaatc aatatcttaa
    12421 aagccatcgg cgctaccctg cctgaatacg gccacctgcc gatgattctt aacgaacaag
    12481 gcaaaaaaat ctccaaacgc agcggcgata ccgttgccat taccgatttc ggcgcgatgg
    12541 gcatcctgcc cgaagccatg ctcaactatc tggcacgctt gggctgggcg cacggcgacg
    12601 acgagttctt tacgatggaa caattcatcg aatggtttga tttgaaagac gtttccccgt
    12661 ccccaagccg tatggatttg aagaaactct actggattaa cggcgaacac atcaaaatca
    12721 ccgccaacgg caaactcgcc gaactcgtca aaccccgcct tgcgctgcgc gatattcatg
    12781 aaaccgaaaa acctgcgttg gaagacgtat tggcactggt caaagaccgc gcccaagatt
    12841 tgaacgcctt ggcagacgaa tgtctgtatt tctacaaaaa acaagtccct gccgaagccg
    12901 atgttgccaa acactgggac gacgaagccg ccgcccgtat gctgcgcttt gccgaacgcc
    12961 tcgaagggct ggaagactgg aatgccgaag ccatccacga cctcttcaaa cccttctgcg
    13021 acgaagaagg cattaaaatg ggcaaactcg gcatgcccct gcgccttgcc gtctgcggta
    13081 cggcgaaaac ccccagcgtc gatgctgtgt tggcattaat cggcaaagaa gaagtgttga
    13141 aacgcatccg cgcttaagtt atccacaggt tcgggtaatg ccgtctgatg ttggaatacg
    13201 gcttcagacg gcatttaaat ggaagttaaa aatgaaaaaa atattttatt ttctgatggt
    13261 tgttttttct accagtgtat gggcagggga tgctgaagac aatttgctca gtatccaatc
    13321 cggttaccgc gccttattgc aaaaacaaaa caagcttgac ggaaaaatta tcgggatgca
    13381 gtcggatttg gaagatgcgc gcagacggtt gcaggcggct caggcagaca tcgcccgttt
    13441 ggaagcggaa attcctgcag caatggcgca aaaagcccgg caggctgaag agctgaggca
    13501 aatcggagtg cgtttggacc atgcttggaa tgcggtttac ggcgcagggg gaacgaaagc
    13561 gtcggggaat tgaccgatga attccctctt tgtggacaat actgttttca ttacacggct
    13621 gaaagccggg catatcggca ggttggttca ggcgttgttt gaggagtggc acggatttga
    13681 accgtggtct tctgtggata agattcgtgc ctattacggc aggtgtttga aggatgacga
    13741 actgccgctg gcttttgcgg ctgtggatga tttcggaatt ctgttgggtt cggctacggt
    13801 caagcggcat gatatggaaa gttttccaca gtatgaatat tggttggggg atgtctttgt
    13861 tttacctgaa tatcgcggaa aaggcattgg caggaggttg gtcgcccact gcataggcgc
    13921 agcgcgttcg ctggggataa agttcttgta tctttatacg cctgatgtgc aaatatttta
    13981 tgaatcattc ggctgggcgg ttgtcgggcg acatttccat aacggtgaat gggttacggt
    14041 tatgcgtttg gatatagata aggtttaagc cgaatggaca aatgccgtct gagctgttcg
    14101 gacggcattt tttatatttg gaaatttcct gtcagataat gtttatccac agatttttcc
    14161 atatggtttg atgctgttcc gacaacggaa attaagatac ggatttgcac aaatctttca
    14221 gacggtacag ttctgacaat gcttcgcgcg gggtcaggct gtcgggatcg agtttttcca
    14281 aggctgctgc caatatacct tcaaaatgtt tttcctctgc tttatccaca aagttgccca
    14341 cattcggttc atctcctttt tcagacggca tggtactgaa aatatccagt tggggacggt
    14401 ttgcggcggc ttggtcttcc agttcgttca aatgcttttg ggcggatttc aatgcgcgta
    14461 caggcaggcc ggcgagtttg gcgacggcga tgccgtagct ttttccggcg ggccccggtt
    14521 ggatttggtg caggaacacg atgtcctgtc cctgttcgag cgcggaaagg tgcatattga
    14581 cggcggcggc gtgggtttcg ggcaggtagg tcagctcgaa atagtgggtg gcaaacaggc
    14641 tgaaggattt gtttttttgc agcaggtgtt cggcaacggc gtgcgcgagg gcgaggccgt
    14701 cgaaagtgga agtaccacgt ccgacttcgt ccattaaaac aaggctttgt tcggtggcgt
    14761 gatgcaggat gtaggcggtt tcgctcattt cgaccatgaa ggtggagcgg ttggaggcga
    14821 gatcgtccga tgcgccgatg cgggtgaaga tttgatcgat gggtccgatt gtggcggcat
    14881 cggcaggcac aaaacagccg gtgtgtgcca ataaaacaat cagcgcgact tggcgcatgt
    14941 aggtggattt gccgcccata ttggggccgg tgagcagcat gaggcggtgt ttgtggtcga
    15001 ggtcggtgtg gttggcggtg aagtggcgta cctgctgttc gacaacggga tggcgaccgt
    15061 tttcgatgtg gataaccgga tagtcggcaa actcggggcg gacgaagttc cgctcttttg
    15121 ccaaggctga aaatgtggac aacacgtcca gcgcggcggc ggctttggcg gctttttgaa
    15181 gctgcggcaa tgccgtctga aggtttttca atacgccgtc aaagagttgt ttttctaagg
    15241 cgagggcttg ctcttgagca gtcagcactt tgtcttcaaa ggctttcaac tccggcgtga
    15301 tgaagcgttc ggcgtttttg agggtttgcc ggcgttggta gtcggcaggt gcttgttcgg
    15361 cttgggtttt ggacaattca atgtaaaagc cgtgaacgcg gttgaactcg actttgagtg
    15421 tggacaaacc ggtacgttcg cgttccttgg cttccaaatc cagcaaaaat tcgtcgccat
    15481 ggttttgaat gcggcgcaat tcgtccagtt cgggatgaaa accgtggttg atgacattgc
    15541 cgtctttcag ccagacggaa ggttcgggca aaatggcttg gcggagctgt tcggctgtgg
    15601 ataggttttc cgggaaaacg gctttgaggg tttccaacag gctgctgccg tttgcggaca
    15661 agtcgatttg tgccaactcg aacagactgt cgcgtaaaga tgccaaatcg cgcgggcggg
    15721 cgttgccgac ggcgatgcgg gcagcaatgc gttcaatgtc cgcaatgttt ttcagacggc
    15781 attggagggg tttgtattgg ctttccagcg cggcaacggc ttcttggcgc gctcggatgt
    15841 gggcgcggtt gcgtaaaggg tggtgcagcc agagagccaa gaggcggctg cccatatggg
    15901 tagcgcaaag gtcgagcgtg gacatcaggg tcggcgattt tttgccggag agggtttgcg
    15961 tgatttcgag attgcggcgc gtggcggcat ccataccgat atattggctg tcggtttcga
    16021 gcgacaggcc gtccaaatgt tgcggcatca ggttttgcgt cagacggata tagttcaaca
    16081 gtgcacctgc cgcgccaatc gcaacggcgt gttctttgcc gtccaaaccg aagccgcgca
    16141 aatcctggca gccgaaatat tccgtcagca gtttttcccc cgcgtcggcg gcaaactgcc
    16201 acgcgttcag gcgcgtaaca cccgatgccg tctgaagttg cggtgcgttt ttactgtcag
    16261 gcaacagaat ttccgccgcc tgcaggcgcg ccagttcgtc gtccaattta tccacagttg
    16321 tcagcttggt tttgaattcg ccgctttgca gcgatgccca cgccaaaccg atgtattttt
    16381 tgtcggggga cacggcaacg atgcggttgg tttccttgtc ttccagcaat gcggaatcgg
    16441 tcagcgtgcc gggcgttacg atgcgcacga ctttgcgctc cacaggccct ttgcccgcgc
    16501 cgacttcgcc gacctgttcg caaatcgcca cgcttttacc caacttgacc aggcgcgcca
    16561 gatattgttc ggcggcgtga aacggcacgc ctgccatttt gaccggctcg ccatccacct
    16621 gtccgcgcgt ggtcagggta atatccaaaa gttttgccgc ttctaccgca tcgtcgaaaa
    16681 acatctcgta aaaatcgccc atacggtaaa acaccagttt gtcggtatgt tgcgctttga
    16741 tgccgaggta ttgctgcatc attggggaaa cggcggattt ggacatgggg ctgccttatc
    16801 attaggaaaa cggtattgta gcaaaaggtt ttgtcttcga cggaatgaga ctgaccgtaa
    16861 gcctgtcaat aaggttgtgg agacaactag gaaaacagca aagttatcca cagaaatatt
    16921 attaagtcaa aaaacatcca cgcttatctg tcttatacag caagtcttaa actcaaactt
    16981 atttgacaaa aaggtaatga aaatattgga tatttgctac ttacgcacaa gcaggttggc
    17041 aatcatcaaa atcatcatta aaattttaaa ttcaaatatt gatggtaaaa gttttggaaa
    17101 tctgtgcaaa cagtaaaatt catctatatc aaataagcgg caagtagtta gatgtaatag
    17161 tggaatgtaa ctttatttca gaatacaata caggtattga aatttcgttt cacagcttgg
    17221 agtatataca tgagcattaa agtagcgatt aacggtttcg gccgcatcgg ccgcctcgca
    17281 ttgcgtcaaa ttgaaaaagc ccacgacatc gaagtcgtcg ccgtcaacga cctgacccct
    17341 gccgaaatgc tgctgcacct tttcaaatac gacagcacac aaggccgttt ccaaggtact
    17401 gccgaattaa aagacgatgc catcgtggta aacggcaaag agatcaaagt tttcgccaat
    17461 ccgaatcccg aagaattgcc ttggggcgag ttgggtgtgg atgtcgtcct cgaatgtacc
    17521 ggcttcttta ccaataaaac caaagccgaa gcacacatca gggccggcgc gcgcaaagtc
    17581 gtgatttccg cacccggcgg caatgatgtg aaaaccgtcg tatatggcgt aaaccaagac
    17641 attttggacg gcagcgaaac cgttatctcc gccgcttcct gcaccaccaa ctgccttgcc
    17701 ccgatggcgg cagtcctgca aaaagagttc ggcgtagtcg aaggtctgat gaccaccatc
    17761 cacgcctaca ccggcgacca aaacaccctt gacgcgccgc accgcaaagg cgatttgcgc
    17821 cgcgcccgag ccgccgcgct caacatcgtg cccaacagca ccggtgccgc caaggccatc
    17881 ggtctggtta tccccgaatt gaacggcaaa ctcgacggct ccgcccaacg cgtccctgtt
    17941 gccaccggct cgctgaccga attggtttcc atcctcgaac gccctgtaac caaagaagaa
    18001 atcaacgccg cgatgaaagc agccgccagc gaatcttacg gctacaacga agatcaaatc
    18061 gtttcttccg acgttgtcgg catcgaatac ggttcgctct tcgatgcgac ccaaacccgc
    18121 gtgatgaccg tgggcggcaa acaattggtg aaaaccgttg cctggtacga caacgaaatg
    18181 tcctacacct gccaactcgt ccgcactttg gaatacttcg caggcaaaat ctaaggcttg
    18241 aacaaacctg tggataagta aatgccgtct gaacacattg cgttcagacg gcattgttta
    18301 tctgtatagt ggattaaatt taaaccagta cggcgttatc tcgccttgcc gtactatttg
    18361 tactgtctgc ggcttcgttg ccttgtcctg atttaaattt aatccgctat aacttgatat
    18421 tgcggcttat ggcgaacaaa caacagccgt ttgttcaact gctatgcggc aaaaccggat
    18481 ttttgcccga tttaaagcgg ggttggggaa agatcaataa cggttgcacg gttttcctcg
    18541 atttgaaatc tgacttcgta atctgcaata ttcatcacat aaatccgttc gggaatattc
    18601 tgataggcgg ggcgcggatc ttgggcaatg ctttggctga taaggttttt ggtgtttgca
    18661 gataaatttt ccgcgccgat gttttcctgc caaacgactt ccaactctac gggtttgccg
    18721 ctgacgaaac cggatgcggc atcgggtttg gattcgacaa aggggatata aggtttgatg
    18781 tccacaatcg gtgtgccgtc cagcaggtct gcgccgctgc aatagaggcg gacgggtttg
    18841 ccggtttcga tgcgttcgag tttcaggagc gagagtccga gatggttggg gcggtggggg
    18901 ctgcgcgtgg cgaacacgcc cattttttgt ttgccgccga gccgtggcgg gcgcaccatt
    18961 tgcgcccagc cttcatccaa tacgccgtga aaaataaaac ttatccacac ataatcgaaa
    19021 tcttccagcc cgcgcacgct gtctgcggtg aatttgggat tcagctcgat gcaggctttt
    19081 gcggcggaga ccaaaccggg ctggcgggcg atgccgaatt tctgtttgta gggcgagcgg
    19141 gcggtgccga tgggggtaat ggtataagtc ataaggttgt ggataagttt tttggaaaaa
    19201 ccttatctta tcacaatcgg ttttaccgct tttgggcagt aatttgttat aatacagggc
    19261 attttaatcg cgttgaaata atgagatgat tgtttccatt gatgttgatg cacaaaaaac
    19321 gtttacgcca ctgtgtcctg acgaactgcc cgtgaacgag gggcatttga ttgtcgagga
    19381 gttgaatgcg caagccgctt tggcggattt gcgcgtgatg acgaaagatg cgcatcatat
    19441 ggtagcgaaa tggcttgtgg ataaccctgt tgatatgttg aagccgacag gtttgcctga
    19501 tgcggatttg acttgggtgg ctcatgcgat ggtcggtacg cgcggctatg aattattgga
    19561 cgggctgcct tctgctaaag aatacgatta ttgcgtttgg aaaggtgttg atcctgaatt
    19621 gcatccttac ggcgcgtgtt ttcacgatat tgaggaaaaa ctaagcacag ggctgattga
    19681 atggctgcgt tgtcaaaata cggatacggt tattatcggt gggttagcta cggattattg
    19741 tgttaaaaca acggttttgc agttactcaa aggcggtcgt tggcaggttg tcgtcaatga
    19801 agcggcttgt cggggtattg cgccggaaac catcgaaaca gcatggcagg aaatgcattc
    19861 tgctggggca atcatcttaa aaaacgctga tgaaataaat aaatatatta ataatcaata
    19921 agttaaaatt gtttcacgtg aaaccccctt cctaaatatg aaaattttgc ttgtccgctt
    19981 gtctagtatg ggcgatttaa ttcacacttt gcccgcaatc gaagatttgg cgcgacaatg
    20041 tcctgatgta gaactgcatt ggctatgtga ggctggattt gcagatattg cgcgcctgca
    20101 tccgtttgta aaaaaaatcc atgtgatgaa atggcggcaa tggcgcaaac atctctttcg
    20161 ggctgaaact tggcgggaaa tgggtcatct gaaacagact ttgcggcagg aagtatttga
    20221 tttcgtattg gacagtcaag gtctgattaa aagcgcgtgt ttcgctaaaa tggcaaaatc
    20281 cccgatttgt ggtttggata aaaacagtgc gcgcgaggga tgggctgctt tggcgtatgt
    20341 aaaaacatac gctgtaccga agggaaaaaa tgccgtttgg cgcaaccgtg aactgtttgc
    20401 ccaagtattt gggtatgtaa tgccggaaac gcaggtattt ggcttgactg ttcctgaagc
    20461 aggccgtctg aaaaatttag cgcagccgta ttatgcggct ttgcatgcga ccagtcggga
    20521 cagtaagtta tggcctgtgg aaaactggcg gtcgttgctg caaaagttga atgaagaaca
    20581 gcaatgcaat atttacctgc cttggggaaa tgaagatgaa aaaacgcgtg ccaaacaaat
    20641 tgcagatggg ctgccgtttg ctattgtgtg cgacaaaata aatttattgc aggcagcgta
    20701 tctgctgaaa tacgcggtcg gaattgtcgg cgtggatacc ggtttgctgc atttggcaaa
    20761 tgccttggaa aaacctgtgg tcggcattta taccgatacc gatccgatta aaacaggcgt
    20821 tcaggtttcg ccaattgcaa aaaatttggg caatatcggg cagattccga ccgcagattt
    20881 ggtttatcaa gcgctgatgg attgtgttgc agcagataaa ggctaaaggg tcgtctaaaa
    20941 ctgatatggc ggcatgatat tttccaattt attagataca ggaatgtata tccgctagtc
    21001 tgagtaaatg ctttaaaaat ggtataatcg gcaagcatta tccgaaagat ttttgaatga
    21061 attcgttttt aaggaaaacg gatttggttt tatagggaag aaggtttggt ttcgctggat
    21121 ggtgaaagca gcgttctttt taggagggtt caatgaaagc actggtcgca gtaaagcgcg
    21181 tagtggacta caacgtcaaa gttcgtgtaa aagccgatgg ttcggatgtg gatatcggca
    21241 atgtcaaaat gtcgatgaat ccgtttgacg aaatcgctgt ggaagaagct gtccgtttga
    21301 aagaagccgg aaaagtaagc gaaatcgtag cggtttcttt gggtgagaaa aaatgcgaag
    21361 aaaccttgcg tacagctttg gcgatgggtg ccgaccgtgc cattcatgtt gaaaccgata
    21421 ctaaactgga gtctctggcc gttgctaagt tgctgaaagc cgttgcggac aaagaaaatc
    21481 cgcaaatttt ctttttgggc aaacaagcga ttgatgatga tgccaatcaa gtggcgcaaa
    21541 tgctggcagc tttgctgaat gcggcgcaag gtacgttcgc gtccaaagta caaattgaag
    21601 gcgacgaagt gcagattgtg cgggaaatcg atggcggcga agaaaccatc gcattgaaac
    21661 tgcctgctgt tatcagcgcg gatttgcgtt tgaacgagcc gcgctttgtc aaactcccca
    21721 atattatggc ggcaaagaaa aaacctttgg aaaaactgac tcctgacgat ttggttgccg
    21781 acatttcacc tcgtttgaaa acggtgaaat ttgccgaacc taaggcgcgt caggcaggcg
    21841 taaaagtagc aagcgttgcc gaattggttg aaaaattgaa aaacgaagcc aaagtgattt
    21901 gaggaggctg aaatgagtgt attgattatt gtcgaacacg acaacaaaca gttgaatcct
    21961 accactttgc atgctgttac cgctgccgcc aaactgggca aagtcgattt attggttgcc
    22021 ggaaacggcg catctgctgt agtggaattc gcgaagcaag tagcgggggt gaaaaaagtt
    22081 ttggttgcag atgctgctca ttatgccgaa ggtttggctg aagagttggc tccgctggtt
    22141 gttaaattgg cggccgatta ccgctatgtt gcagcaacgg caaccacatt cggtaaaaac
    22201 cttttgcccc gcgtagcagc cttattagac gtaccgcaaa tttctgattt gaccgaaatt
    22261 gtggataaca cgacttttgt gcgccccatt tatgcgggta atgcatttga aaccgtgcaa
    22321 gttgattcgg aaaaattggt gctgaccttc cgtgcgacgg cttttgatgc agctcctgta
    22381 caaggtggtc atgccgaggc agttaatgtt gaagcaaccc ctgcccaaaa cctgagtcgt
    22441 tttgttaacc gtcagctttc ccattccgat cgtcccgaac tgactcaggc aaaagtgatt
    22501 gtttccggtg gtcgtgcgtt gggtagtgcg gaaaaattta atgaagtgct gacaccgttg
    22561 gcggatgttt tgggtgcggc aatcggtgca tcccgtgcag cagttgatgc cgaatatgcg
    22621 ccaaacgatg ctcaagtcgg acaaaccggt aaagtggttg cgccgcaact ctattttgca
    22681 atcggtattt caggtgcgat tcaacacgtt gccggtatgc aagacagtaa ggtaattgtt
    22741 gcaatcaata aagatgctga tgcgccgatt ttcaatgtag ccgattacgg attggttggc
    22801 gatttatttg aaatcgttcc acaattaaca gaagcattga aaaattgatg tttcacgtga
    22861 aactttcaga cgaccttgtt attaatgagg tcgtctgaaa acatttcagc atgattacga
    22921 cttataaaac cattacctcc ttgacgcagg ctgagtttaa agataaaggc agccgtttta
    22981 ttgcatttgc ctatccgatt cggacattgg ctgatgtgaa aaaatacctc gatccgttaa
    23041 aggaagagca tcataaagca cgacactggt gctatgccta tcgtttgggt gtggatggtg
    23101 tgcaatttcg tgccaacgat gatggagagc catcaggaag tgctggacga ccaattttgg
    23161 gacagattga ttcggtgggt attaccgacg ttttggtcgt ggtcgtccgc tatttcggtg
    23221 gtactttgtt aggtgttcca ggtctgatac atgcgtacaa agaggcgacg gctcaagcgt
    23281 tggcagttgc agaggtagtt gaaaagaata ttgaaaaaac tgtttggctg aaatgtgaat
    23341 atccgttttt gaatgaagcg atacgcattg ctaaacaata tcaggcggat gtattggagc
    23401 gggatttgca gttggattgc cgattgacgg tcagtttgtc gttggtaaat tatgaggcct
    23461 gtgttgcggc ttggaagagt acacggcaga ttgaagtaaa tacggaaaag ccttttgaat
    23521 gaaaggtcgt ctgaaatgct ttgtgatggt atttcagacg acctcttcgc tgactaatct
    23581 tcttgtgtat atttgtctaa ggcatcgacg ctggagcgca aatataacca agccagttga
    23641 tggaattcgc ccagcgcgtt ccatagtgca tcttcactca taatgctgta ttcgccttct
    23701 gtgcgcaaat ccacctccgc tccatcttca atagcccgat gacaagcctg atattcgtgg
    23761 gtataaaaat tgtccatatc gcgaatcaaa gcgacggcat gtttccaacg gcggatgtct
    23821 tcttccaatt gcggtagtaa atgacaccat tcgcggtatt tgctttggat ttcttcaata
    23881 cgtttttgca tggttgcttg tcctttttta atttatgaat aagttgaata ggttgcagag
    23941 tggaatgaag cggtcttttg tttgattcta ctaaaatttc ttcagatggg ataaaagtcc
    24001 ctttgtatcg agttttcgtt aaaagtacat atttgtcaag gagatggcat gaaactgctg
    24061 gttatcggta atggcggtcg cgaacacgcg ctggcttgga aattggcgca gtcgcctaaa
    24121 gtggaaacag tatttgttgc gtctggtaat gccggtacag cgattgaacc caagctgcaa
    24181 aacatcgact tgactgcgca tcaggatttg attgaattct gtcgtaaaga aaatattgtt
    24241 tttaccgtcg tcggccctga agcgcctttg gcggcaggtg ttgtggatga tttccgtgct
    24301 gcaggactaa aaatattcgg tccgacacaa tatgcggcgc agttggaaag ttccaaagat
    24361 ttcgccaaag catttatggc gaaatacaat attccgaccg cgcaatatca aacctttgaa
    24421 aacgccgatg ccgcacatga ttacgtcaat cagaaaggtg cgcctatcgt tatcaaagcc
    24481 gatggtttgg cggcaggtaa aggcgtgatt gtggcgatga ctttggatga agcgcatgct
    24541 gcgattgatg atatgctgct ggacaacaaa atgggcaatg ccggcgcgcg tattgtgatt
    24601 gaagatttcc tgcaaggcga agaagcgagt tttatcgtca tggttgatgg caataatgta
    24661 ctgcctatgg caaccagcca agaccacaag cgtcttttag acggcgacaa aggtctgaat
    24721 acgggcggta tgggtgcgta cagccctgcg cctgtggtaa cgcctgttgt gtacgaacgc
    24781 gcgatgaatg aaattatttt gccgaccgta gcagggatga aagcggaggg gcatgagttc
    24841 accggtttcc tgtacgcagg tttgatgatt gatcaaagcg gtgcacccca tacgattgag
    24901 tttaactgcc gtttcggcga tcctgaaacc cagccgatta tgagccgact gaacagcgac
    24961 ttatcggatt tggttgaagc ggcaatagac ggcaaactcg atagcgtaac cgcggaatgg
    25021 agcccgcaaa ctgccgtggg cgtggtactg gcggcgcaaa attaccccga aacgcccaaa
    25081 aaaggcgatg ttatttccgg tctggatgct gccaaccaag taggcaaagt tttccatgca
    25141 ggtacaacgg caaacgagaa aggtgacgta ttgaccaacg gcggacgcgt attgtgtgtt
    25201 gtgggattgg gcgataatgt ggcgcaagcg aaagctaagg catatggcgc gttggaaaaa
    25261 atcagtttcg acgggatgca ataccgtaaa gacattgccg ataaagcgat taaccgttaa
    25321 ttagaaagag gatgatgccg tctgaagcca agtatgacta atgtgcttca gacggtattt
    25381 tatattatgt tatttccgag aattatcgca gcatcagcgc aacgtaagtt aagcgtttat
    25441 ttgaaaaaag gtggtttggt cgcttatccg accgaatctt gttatggctt ggggtgtctt
    25501 ccgacattag ccaaagcact cggtaagttg gcgcatttga aaaaaagacc gcaacacaag
    25561 ggtatgattg tcatcgggaa tcaatttgaa caattgcagc ctttacttca aatgccgtct
    25621 gaaaatcttc aggatatgct tagaaaagaa tggcccgccc ctaaaacgtt cctattatcc
    25681 gccaaatcct gtgtcttacc cgagttacgc gggaaacagc gcagcaagtt ggcggtcagg
    25741 gttcccgccc atgtaggtgc gcgccgcttg tgtcaggctt tgcaaacgcc tttggtctcg
    25801 acttcgtgca accgcgcagg caaacgcgcg tgtcggacgg agagggaagt cagaaggcaa
    25861 ttcggacgcg atgtgtggat tgttggtggg agaatcggca ggcagaaatc gccgagtcag
    25921 attattgacg gggaaacagg taaacgtttg cgttagatat gggtacttga atgaaaactt
    25981 tttttgcata cacaaagaaa aaatttttcg gatagcattt ctctacatga ttgttattgc
    26041 acagagataa aggtagaagg acgcaacgtc tgctttaatt ttgaggacgg ttttgtcgtt
    26101 ccggacaata atccgaacaa tcaagcgggc aagcatctga aaacagattt ttcccgtgtt
    26161 gttttaatcc ataaaaatga aaacgccgaa gatgatta
//
LOCUS       DAXHNE010000015        22679 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.015, whole genome shotgun sequence.
ACCESSION   DAXHNE010000015 DAXHNE010000000
VERSION     DAXHNE010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22679
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.015"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            1..99
                     /locus_tag="ACFM4Z_000457"
                     /pseudo
     CDS             1..99
                     /locus_tag="ACFM4Z_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(120..1034)
                     /locus_tag="ACFM4Z_000458"
     CDS             complement(120..1034)
                     /locus_tag="ACFM4Z_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6020552.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="ACFM4Z_000459"
     CDS             1290..2759
                     /locus_tag="ACFM4Z_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HFC6020553.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="ACFM4Z_000460"
     CDS             3011..3382
                     /locus_tag="ACFM4Z_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020554.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVG
                     SQCRAELDKRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMTGNDVMQMLAPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..4864
                     /gene="thrC"
                     /locus_tag="ACFM4Z_000461"
     CDS             3455..4864
                     /gene="thrC"
                     /locus_tag="ACFM4Z_000461"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFC6020555.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDENIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFNATSSNDETVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPA
                     EINTLWAEVAAGKGFDLRFALDKVGGKYGFTSGKSTHADRLATIKQVYEQDQELIDPH
                     TADGVKVAREVREEGEMVVCLETALAAKFDATIREAVGDAAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4910..5704
                     /locus_tag="ACFM4Z_000462"
     CDS             4910..5704
                     /locus_tag="ACFM4Z_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020556.1"
                     /translation="MTLPMQETRFSILLDELAAKQEAAIAPHLLADGTKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRSAGIAVPELLAVRK
                     NALMFGNLEGIPLDAQIRQEAEAGKSDAWLAGLEAIARVHKKQQFLSQAFARNMMWDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            5892..6668
                     /locus_tag="ACFM4Z_000463"
     CDS             5892..6668
                     /locus_tag="ACFM4Z_000463"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC6020557.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            6744..6850
                     /locus_tag="ACFM4Z_000464"
                     /pseudo
     CDS             6744..6850
                     /locus_tag="ACFM4Z_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(6921..7064)
                     /locus_tag="ACFM4Z_000465"
     CDS             complement(6921..7064)
                     /locus_tag="ACFM4Z_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020558.1"
                     /translation="MENNKVKSDYAAKQESLGKVDENKNETMKIPPQVGTHMLGFSFI
                     FKC"
     gene            complement(7741..10212)
                     /locus_tag="ACFM4Z_000466"
     CDS             complement(7741..10212)
                     /locus_tag="ACFM4Z_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC6020559.1"
                     /translation="MKKTCFHCGLDVPENLHLTVRYEGEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRAGMDTPIAVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHRMPGYPAVQDVREAAVVKLQ
                     AGDVVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRVSSGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LVVLGNAMRLHKQVDMPSEKMPSEQ"
     gene            complement(10209..11387)
                     /gene="hflX"
                     /locus_tag="ACFM4Z_000467"
     CDS             complement(10209..11387)
                     /gene="hflX"
                     /locus_tag="ACFM4Z_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFC6020560.1"
                     /translation="MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSATRLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLVNLKKQRALRRKPRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(11392..12672)
                     /locus_tag="ACFM4Z_000468"
     CDS             complement(11392..12672)
                     /locus_tag="ACFM4Z_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFC6020561.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGSGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            12674..12802
                     /locus_tag="ACFM4Z_000469"
     CDS             12674..12802
                     /locus_tag="ACFM4Z_000469"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020562.1"
                     /translation="MPSSRRGNGVFYQNGKLANAVSACRFPNRQTFPRADAEPDAV"
     gene            complement(12825..13550)
                     /locus_tag="ACFM4Z_000470"
     CDS             complement(12825..13550)
                     /locus_tag="ACFM4Z_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFC6020563.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(13623..14300)
                     /gene="radC"
                     /locus_tag="ACFM4Z_000471"
     CDS             complement(13623..14300)
                     /gene="radC"
                     /locus_tag="ACFM4Z_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFC6020564.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSF
                     RQLGLMP"
     gene            complement(14423..15772)
                     /gene="gshA"
                     /locus_tag="ACFM4Z_000472"
     CDS             complement(14423..15772)
                     /gene="gshA"
                     /locus_tag="ACFM4Z_000472"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFC6020565.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFSKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            16032..17441
                     /gene="leuC"
                     /locus_tag="ACFM4Z_000473"
     CDS             16032..17441
                     /gene="leuC"
                     /locus_tag="ACFM4Z_000473"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFC6020566.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLKAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17538..17792
                     /locus_tag="ACFM4Z_000474"
     CDS             17538..17792
                     /locus_tag="ACFM4Z_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020567.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17854..18495
                     /gene="leuD"
                     /locus_tag="ACFM4Z_000475"
     CDS             17854..18495
                     /gene="leuD"
                     /locus_tag="ACFM4Z_000475"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFC6020568.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEERVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPSGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18670..19940
                     /locus_tag="ACFM4Z_000476"
                     /pseudo
     CDS             18670..19940
                     /locus_tag="ACFM4Z_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            19956..20687
                     /locus_tag="ACFM4Z_000477"
                     /pseudo
     CDS             19956..20687
                     /locus_tag="ACFM4Z_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
     gene            20721..21791
                     /gene="leuB"
                     /locus_tag="ACFM4Z_000478"
     CDS             20721..21791
                     /gene="leuB"
                     /locus_tag="ACFM4Z_000478"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFC6020569.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDAGYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLNILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            21954..22517
                     /locus_tag="ACFM4Z_000479"
     CDS             21954..22517
                     /locus_tag="ACFM4Z_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFC6020570.1"
                     /translation="MKKIIFAALAAAAVGTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMAKTEVCGGDFSTTIDRT
                     KWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5531 a         5915 c         5833 g         5400 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagt
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcagaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatggtta tcaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc ggcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttatggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ccttcgcgtt
     2941 atcccgaacg gggcggctaa tcagattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggcggctg
     3061 cgccgccgga ggcggtaaca cattcggcag cttagacggt ggcacaggca tgggcggcag
     3121 catcgtcaaa atggcggttg ggagccaatg ccgtgcggaa ttggacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgccga aaaacaggcg gagtgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cacccgaacg gatgaccggc aacgatgtga tgcagatgct
     3301 ggctccgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatgc cgtctgaagc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tacatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg cttgcccccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgcgaaacc ttggacaaat ggcgcggttt
     3601 agcttatccc gaattggcgt ttgaaattat gcgcctgttt gttacggata ttccggagga
     3661 cgatttgcgc gacattctga accgtactta cacggaagcg gcgttcggta ctaaggaaat
     3721 cacccccgtc cgcacgcttt cagacggcat caaaatccaa gccttgtcca acggcccgac
     3781 gctggcgttc aaagatatgg caatgcagtt tttgggcaat gcgtttgaat atgttttaaa
     3841 caaagaaggc aaaaaactca atatcttggg cgcgaccagc ggcgatacgg gttcggctgc
     3901 ggaatatgcc ttgcgcggca aaaagggcgt gaacgtattt atgttgtcgc ccgacggtaa
     3961 aatgagcgcg ttccaacgcg cgcaaatgta cagcctgcaa gacgagaata tccacaatat
     4021 cgccgtgaag ggaatgtttg acgactgtca ggatattgtg aaggcggtgc agaacgatgc
     4081 cgcgttcaag gaaaaatacc atatcggtac ggtcaactcg atcaactggg gacgcatcgt
     4141 cgcgcaagtg gtttattact ttgcaggcta tttcaacgcc acttcaagca atgacgaaac
     4201 cgtcagcttc tgcgtaccga gcggcaactt cggcaatgtt tgcgcgggac acattgccaa
     4261 acaaatgggc ctgcctatcc gccgcctgat tgtcgcgacc aatgaaaacg atgtgttgga
     4321 cgagtttttc aaaaccggtg cataccgccc gcgcaacagc gcgcatactt atgtaacctc
     4381 cagcccgtct atggacattt ccaaagcgtc caacttcgag cgtttcgtgt tcgacctgat
     4441 ggatcgcgat cctgcggaaa tcaatacgct gtgggcggaa gtggctgcag gcaaaggctt
     4501 tgacctgcga tttgccttgg acaaagtcgg tggcaaatac ggttttacct ctggcaaatc
     4561 cacccacgcc gaccgcctcg ccaccatcaa gcaggtttac gagcaagacc aagaactcat
     4621 cgacccgcac actgccgacg gcgtaaaagt cgcccgcgaa gtgcgcgaag aaggtgaaat
     4681 ggtggtttgt ttggaaaccg cgttggcggc gaaattcgat gcgaccatac gcgaagccgt
     4741 cggcgatgcc gccattccgc gccctgccgc gctggaaggt ttggaaaacc tgccgcagcg
     4801 cgtgcaaacc gtgccgaaca gtgcggatgc ggtaaaaggc atcatcgaac aaacccttgc
     4861 ctgatattcc gtacaggggc attccgtccg aacttaagat tgaaagaaaa tgaccttacc
     4921 tatgcaggaa acccgttttt ccatactgct agacgaattg gcggcaaaac aggaggcagc
     4981 catcgccccc cacctgcttg ccgacggaac caaggtatgg atacgcaaag caggcaggca
     5041 caatgcacga tggcgttacg cgctgctcgg tatggttgcc cgatatttga aactgggggt
     5101 gttgaagccg gttcccagcc tcggcggcga gcctgccatt gcaaccgaat caaaacgcct
     5161 gtacgaattg cgctcggcgg ggatagccgt tcccgaattg ctcgccgtca ggaaaaatgc
     5221 ccttatgttc ggcaatttgg aaggcatccc gctcgatgcg caaatccgcc aagaagccga
     5281 ggctggaaag tcggacgctt ggcttgccgg tttggaagcc attgcacgcg tgcataaaaa
     5341 acagcagttc ctcagtcagg cgtttgcacg aaatatgatg tgggacggaa aaaacatcag
     5401 ctttttggat tttgaagacg atccttccga agtcctgacc atagcgcaat gccaagcccg
     5461 cgattggctc tgctacattc attctaccgc gctaatattg aaaaacggcg gactgctgga
     5521 agctgctgcg gaaaaatggg gcggcgtatt gtcggatcag cctgccgaaa tacaaaagct
     5581 gattgccgcc accgtcaaac cgatcctccc gatacgcagg ctggaacacc cccgctgggg
     5641 acgggacgcg ctcaggctgg cagcctcgat ttccctgatt tcccttgccg atatgccgcc
     5701 ataagccgcc gcccgcccca cgatgccgtc tgaagccctt cagacggcat ataaccaacg
     5761 cgcatcaagc attgtcgcaa ccatccgttt cgtttagaat tttgacttta tcccgaattg
     5821 ggcagatatt ctcattctga cacacgtccc aaataaacac tccccatttc ttttaccgac
     5881 aggaaaccca aatggcagca ttcaataccc aaaaagtatt gtccgtacac cactggacag
     5941 acgcatattt tacctttacc tgcacccgcg acgaatcgtt gcgctttgaa aacggccaat
     6001 tcgttatggt cgggctgatg gtggacggca agccactgat gcgcgcatac agcgtcgcct
     6061 ccgccaactg ggaagaacac ctcgaatttt tcagcattaa agtccaagac ggcccgctga
     6121 ccagccgcct gcaacacctc aaagtcggcg acgacgtgtt aatcagcaaa aaaccgaccg
     6181 gaaccttggt tgccggcgac ctgaatccgg gcaaacacct ttacctgttg agcaccggta
     6241 ccggcatcgc ccctttcttg agcatcacca aagatcccga aatttacgaa cagtttgaaa
     6301 aaatcatcct cgtacacggc gtgcgctaca aaaaagattt ggcgtactac gaccgcttta
     6361 ccaaagaatt gcccgaacac gaatacctcg gtgacttggt taaagaaaaa ctgatttact
     6421 acccgattgt ttcgcgcgaa gaattcgaac accacggccg cctgaccgac ctgatggtga
     6481 gcggcaaact gtttgaagac atcggcctgc ccaaaattaa tcctcaagac gaccgcgcca
     6541 tgctgtgcgg cagccccgcg atgctgaaag acacctgcaa agttttggac gatttcggac
     6601 tgaccgtctc tccgaaaaca ggcgtacgcg gcgattacct gattgagcgc gcatttgtgg
     6661 accaataatc tgcttgaaaa tgccgtctga acggcatctg cgataaaaaa ccccgaatcc
     6721 tgccaatcag gattcggggt ttttatagtg aattaaattt aaattaggac aaggcggcga
     6781 gccgcagaca gtacaaatag tacggcaagg cgagccaaca ctgtaccggt ttaaatttaa
     6841 ttcactatat agtgggtaaa aattagaaat gcacatttcc gtcattcacg cgcaagtggg
     6901 aatccagacc tatccgttaa ttagcatttg aaaataaaag aaaaaccaag catatgggtt
     6961 cccacttgcg ggggaatctt catcgtctca tttttgtttt catcaacttt tcctaaactt
     7021 tcctgtttgg ctgcataatc agacttgact ttgttatttt ccatattgag cttctcttaa
     7081 tttgaaaaaa ttaacataaa catttaaaaa taaaacaaaa ttatataata aaaaaaataa
     7141 tgagaatata aaggataaaa agagagttaa gttatttaat cggacttgtt ctctataaat
     7201 cataagacac caaataagcc ataataaatt tagtaaataa aaactgctta tacccaactt
     7261 tcttttcttg gaacctatta ttatttcaac tccggatgaa aaaatagaat ttaaaaattt
     7321 ttttctttga tatatcgtta atatctccct agtttttctt aaaaaaaata taatcatata
     7381 tatactacac aacattggta ataaataaat taaaagcgaa aggtaattaa attgataatt
     7441 gtaaattata taataaacaa catacgaaga aaaaattatt atagatgatt tactatacga
     7501 ataaaaaaat atcctaccta gcccgaacct tcttataatt tgtactatgt gattatccat
     7561 ttcaatatcc atttaagctt tcaattaatt ttctatattt ttcaatggca tgtatcatgc
     7621 tatgcttggg aaatatcatc ttggttgatg atcaagccat gtatgcaaag attggaatat
     7681 ggtatttgtc gtgctaataa tgcccccgtt actgttatac gtctaaagca acgctgtccg
     7741 tcattgttcg gacggcattt tttcagacgg catgtccacc tgcttgtgaa gccgcatagc
     7801 attgcccaat acgaccagcg aactgaagct catacccagt gcggcaatcc acggttggac
     7861 atagcccaaa acggcaagcg gtacggcaat gatattgtac gcgcccgccc atatcaggtt
     7921 ttgccggata atatggcggg tgcgccgcgc ctgatcgagc aggtgggcga cggtacgcaa
     7981 atcttcgttc aataacacaa tgtccgcgcc gtccctcgca atatccgtcc cgcccgctgc
     8041 ggcggcggat acgtctgcct gcgccaaaac gggcgcgtcg ttgatgccgt cgcctatcat
     8101 cagcactttt ttcccttctt tttgcaaggc tttgacgtat tccagtttgt cctcgggcat
     8161 ggcttgggcg cggtagtgcg cgacacccag ggcgcgtgcg gtttcggcaa cggcggtttc
     8221 gcggtcgccg ctgaggatgt gcagggtcag gtttttgcct gccaactgcc gcaccgcctc
     8281 cgccgcgctg tctttcaagg ggtcggtcag gtagaacacg gcttggaaac cgctttgact
     8341 gccgaggtaa accgcgctgc cgccgccttc tgtttgcggt tctttacctg aaatttcggc
     8401 gacataggat gccctgccca atgcccaaac ctgtgtttcg ccgttgacgg tcagttgcgc
     8461 gcccacgcct tcgccgatgc ggttgaggcg ttgtttaata gcgatgtcgg ggacgctgcc
     8521 gcttgaaaca cggcagttga ggatggcgcg ggcaagggga tgttcggact gttgttctaa
     8581 agcctgcgcc accgcgagaa caaaggcttc gtctgtgcct ctcaacaatg agatacggcg
     8641 gacggcgggt ttgccttggg tcagcgtgcc ggttttgtcg aagatgatgt cggtggtttg
     8701 ggcgagggtt tcgattgcct gctttccgcc gattaaaatg ccttcgcgcg ccagcgtacc
     8761 ggtagaagct gccagcgcgg tcggcgtggc aagcgataag gcgcaggggc aggtaatgac
     8821 cagcagggcg acggtaatcc acaatgcggt gtgcgcgtcg gcgtacagcg tccagccgat
     8881 gaagacgggg acggcaagca ggagttcgcc gaatatgaaa gacgaggcgt attgttccgc
     8941 caactcggca gtgcgcggtt tttgcgctaa ggcgcggtcg agcaggcgga cgatgtgcga
     9001 cagtcgcgtg ccgccgccgg tgcggtcggt gcgtataatc agggggcttt gcgtgttgag
     9061 tgtgccggcg gttacttttt cagacggcat tttggcgacg ggcaggctct cgccggtcag
     9121 catagattcg ttgacggcac tgcttccttc cagcaccgtg ccgtcaacgg ggatggtttc
     9181 gcccggtttg accagcacca catcgcctgc ttggagcttg acgacggcgg cttcgcggac
     9241 atcctgtacg gcaggatagc cgggcatccg gtggcagaag gcgggtatca gcttcaccag
     9301 cctttcggcg gcatcgcctg ccttgcgtcg ggcaatgtgt tccataaagc gtccgcccag
     9361 caggaaaaac agcagcatgg ctatggattc gaaatacatc ccctgccccg cattggtcgc
     9421 aaggctgtaa acgccggcga taaaggtcat gatgatggcg acggcaatcg gcgtatccat
     9481 gccggcgcgg cggtttttca ggtcgcgcaa cgcgccttga taaaacggga cggcgcaata
     9541 gaatacgacg ggcagcacca ttaaaaagcc gccccaatgg aggatttgca ggaaatcggg
     9601 ttcgatgtcg ccgccgtaaa ggtaggtcgg cagcgcgaac atcatcgtct gcatcatccc
     9661 cagcccggca acggcgaggc ggacgatgta ttgtttgcgt tctttttggt tggcggcttc
     9721 gattttttgc gcgtcatagg gtgcggcggt gtagcctatc tgccggattt tcaacagaat
     9781 atcggaaagg cggattttgc cgtcgtccca gacgacgcgg cagcggtgcg tgctgtaatt
     9841 gaggtcgatg cggacgatgc cgtctgtacg caaaagctgc tgttcgatca gccagacgca
     9901 ggcggcgcag gtgatgccgc cgagcattaa aaccgcctcg cgcgtgccgc cgtgggtttc
     9961 cacaaagtcg gactggactt cgggcaggtc gtacaggcgg atttggtcga ggatttcttg
    10021 gggcggcagc tcggtttttt gcgcgtcggc ggtgcgttgt ttgtaataac tgcccaagcc
    10081 cgcgtcaata atgctttgtg cgaccgcctg acagccggcg cagcaggttt ctcggtcttc
    10141 gccttcgtag cggacggtca gatgcaggtt ttcgggaaca tccagcccgc agtggaaaca
    10201 ggtttttttc atggcatttc ggtttcgtct gtgtttggtg cggcggcaca agactcggca
    10261 atggcttcgc gcagtgcgtc tataccggta ttttcagcaa cggaaatgcg gacggcggca
    10321 atttttcccg cagcgtcgcg ccatatgccc gtgttttgtt cttcagacgg cagcaggtcg
    10381 gttttgttgt acaccttgat gcacggaata tcgccggcat ggatttcttg cagtacgttt
    10441 tccacgtctt caatctgctg tccgctgttc ggggcggcgg catcgacgac gtgcagcagc
    10501 acatcggctt gcgcggtttc ttccagcgtg gcggaaaatg ccgaaatcag tttgtgcggc
    10561 aaatcactga cgaatccgac ggtatcggtc aggataatgc tgcattcggg actgatgtac
    10621 agccgccgcg ccgtggtgtc gagtgtggcg aaaagctggt ctttcgcata tatgcccgac
    10681 ttggtcagcc ggttgaacag gctggatttg ccgacattgg tatagccgac cagcgcaaac
    10741 gttttgattg tgcccgattc gcggggcttg cggcgcaggg cgcgctgttt tttgaggttg
    10801 acaagctgtt ttttcaaggc attgatccga tgggcgatca atcggcggtc ggtttccagt
    10861 ttggtttcgc cggggccttt catgccgata ccgccgcgct ggctctgcag atggccgtaa
    10921 ccgcgtatca agcgtcccgc caaatggctc aattgcgcca actcgacttg cagcctgcct
    10981 tcctgcgtgc gggcgcggcg ggcgaaaatc gccagaatca gccctaccct gtccaatacg
    11041 cggcatttca gttctttttc aaggttgcgt tcctgcgtgg gcgtgagttc gtggttgaat
    11101 acgaccaaat cgatgccgtc tgcggcaact gcttctgaca gctccgccgc cttgcccgtg
    11161 ccgacaaaca gcgcggtgtg cggacggtcg cgtttggcag tctccacgcg cacggaatcg
    11221 ccgcccgccg ctttgaccag ctcgacagct tccgccaatg ccgtctgaaa accgttcaga
    11281 cgggtggcac tgctgcccgt gccatctttg tccaacatta cgcccaccag catgacgcgt
    11341 tcgggttgcg cttgggtact gccgttgcgg cctgttctgc ctgtcatgta ttcaatcctg
    11401 taaataaagg gttacgcctc gttccgtttc tccttccggt gcaagcgtga tgcggctgcc
    11461 cgccattccg tcaacgctga agacgatgcc gcccgcctcg tgccttccta tggacaggcg
    11521 gctgccgggt cgctgccggt aggtataacg ggtacacgct tccaaacacc agcgtccgcg
    11581 ccagtggatg aaatattttt cgccttttct aaccgcaagt gtctcgccgc cgtttacctc
    11641 aaaataaggg taatcgggtt ttcgggttct aaactgccat ttcgcctgcg cgcgccgtcc
    11701 gttgcggaca tagtcgaata ccgccgtata aagcgtaccg tattccaaag gtttgagcgg
    11761 aaaaagcgcg aattggtacg cggtcagcct gccgttgggg tcgttgccgg cggttaaaac
    11821 cctgacgggg cggatttcgt ttttaccctg atacagcttg aaacttttca tcgtaatttt
    11881 gcctgccgcc tcggaaaaat caatgctggc aggattgccc gtgatttcat attccggcac
    11941 ggggtctgga cgctcgccgt ggaaataagg cagtgcgccg ctgccgacgg gataggctgt
    12001 atagagcagc tcctgtgcgg gcatggcttc gtcggtgtac acgaccgcac cgttatggca
    12061 ggcgttgcgg taatatttcc gtcctgcttc cggctgattt ctgccttggg cgcaatgccg
    12121 ctcaaacctg ccgttgccct gattgaatac gagaacggtt ttaccgtttt cgcgcacaaa
    12181 tgccgctcct gcctcatccg tatggcggtc aagtagggaa aggcggtggt agattgcgct
    12241 cattaatccg tccacttggc gttgctgcgt gcggatgtcg ctgtcggacg attcggcggc
    12301 ttcctcttcc gtgctgatgt tttcatgcac gccgttgtag agatacccgg caaggcgtgt
    12361 gcgttcggtc agcttttgtg cggtgtagtg cggattgtcg ggatggtgtt cgccgtgtcc
    12421 gtcttcggga ttgagcgtga gatagcgtgc gtgcctgcgg gcggaatttt ccaaaaccgg
    12481 cgcgtgtgcc agcttgtgca aaccgatttg ggcgcggatg cggttgaggt agtgcagcgc
    12541 atcgccgccg tccctgattt gcggtgcgga cggatagaca agttcgcccg cgggcaggga
    12601 ttggttttgg gtatgataga aaacgcctgc cgccgaaccc aacaggagca gccgaataaa
    12661 aagggatttc ataatgccgt cctcccgacg cggcaacggg gtgttttatc aaaacggcaa
    12721 acttgccaat gcggtttccg cttgccgatt cccaaaccgg caaacctttc cccgtgccga
    12781 tgccgaaccc gatgccgtct gaaccttcag acggcatcgg gtgtttattt gtccacccgg
    12841 atgggtgcag gttcgtattg tgtcgattcg tcgccgtaat acagcacgcc gagtttgatg
    12901 gggatgcgtc cctgcgattt gcggtgggca ttggaatccc tcagggaata ggcgcaaccg
    12961 caatattcct gctggtagaa gttttcgcgt ttgctgattt caatcatgcg cgcgctgccg
    13021 ccgcctttgc gccagttgaa atcccaatac accacatcat cgtaaggcgc ggcggcgcgg
    13081 tgtccgcagt cgttgatttg cgccatattt ttccagcgtg aaatgcccag cgaactggta
    13141 aagacgggga acccgtgttc atgcgcgtat tgcgcggctt tttcaaaacg catatcgaaa
    13201 cacatggtgc agcggatgcc gcgttccggc tcaaactcca tgcctttggc tttggcaaac
    13261 cattctttgc ggtcgttttc gtagtcgtcg tctttatcga tgaaaggaat gccgaacttt
    13321 tccgcaaagc gcacgttttc ctcttttcgg agcatatact ctttatgcgg atggatattg
    13381 ggattgtaaa aataaatggt gtaatcaatg ccgctggcca gcatggcttc catcacttcg
    13441 ccgctgcacg gggcgcagca ggaatgcagc aagacttttt tatgtccgcc gggcgggacg
    13501 agtatggggc ggtcaatgtc ggtaacggta ggtttgtttt gtgtttccat taacttaaca
    13561 atatctgtcg aatacggttg aagcggctat tttatcagag ccgccgcatg taaaacaggg
    13621 tgtcagggca tcagcccgag ctgcctgaac gaacggacgg tttgcgccgt tacgataaaa
    13681 tggtcgagca gcgacacatc gaccagcgac atggcctgtg ccagccgcct tgtgaacatg
    13741 atgtcttcct gcgaaggttc gggcgagccg cccggatggt tgtgcgcaat aatcaggctg
    13801 tcggcatatt cgtccaatgc cagtttgacg atttcgcgga tgtaaatcgt gttttccgca
    13861 accgtaccgc gcgacagctc tctgaccgca atcagttggt tttggcggtt cagcagcagc
    13921 gcgacgctga cttcgacttt ttcctgcccc aaatgaaagc gtaaataatc ggcaacggta
    13981 tccggatcgg acaggacgat gctctcctgc aattcttccg ccaatatccg ccgcccgatt
    14041 tccctgacta cggcaaactg tgtgaaactt gccgtcccca tccctttgta tgccgacagt
    14101 ttgccgacct ccgcgctcat cagcctcccc aaactgccga actcctgcag caaataacgc
    14161 gccaaatcga ccgcactcat tccgcgcgtg ccgacgcgca gcaggattgc caaaagttcg
    14221 gcatcactca aagccgccgc cccgcgttcc aacagctttt ccctgggtct ttcgccttcc
    14281 ggccattgct tgatgctcat gttttccctt ttttaaatag tctgagatgc cgtctgaaac
    14341 aaatgttccc acacgccaaa tatccgaaat gccgtctgaa ccggatttcc gcttcagacg
    14401 gcatctgtat ttcaaaacag tcttacgccg tttcttccaa ctcgatggac gccgccagaa
    14461 gcgacaggcg tgccattacg ccgtaaacgt agaggcggtt gtgttcgttg tccacgtcgc
    14521 agtctttttc agagcgcggc atacccagcg agtcccattg ttcgaatacg cgcttgcagg
    14581 cttcgggagc ttcttgggag ttgtcgccgt tgccggtggg gatgctgttg gacagcggaa
    14641 caaacaccat accgccggcg ttgaggtttt cgtccgcacc gcgcccttcg tgtacgcgga
    14701 aaaagccgcc gatgacgaag cggtccatca tatagacgac gggttcgcac accgcgccgt
    14761 ttaaggtttc ataagtataa atcccttctt ggacaatcac ttcgctgact tccaagcctt
    14821 ctttgacttt cgccatttta ttgcggtttt tacggttcaa tccgcgcact tcgtcggcgg
    14881 atttgacgct catcacgccc atgccgtaag tgccggcatc ggctttgacg atgacgaaag
    14941 gtttgtcggt aatgcccgat tcgtcgtatt tggcttgaat tttcgccagc acacgttcta
    15001 ccgcttccgc caacgcgtct tcgccttcac gcccttggaa gtccaaaccg ccgatttttt
    15061 caaaataagg gttaatttgc cattcgtcga tgccgattaa cttggaaaat tcggcggtaa
    15121 cttggttgta cgcgccgaaa tgatttgttt tgcggcgcgt cgtccaaccg ccgtgcaacg
    15181 gcggcaaaac ggtttggctg atgcctttga ggatgtcggg aatgccggca gacaaatcgt
    15241 tgttcaacaa aaccacgcaa ggcgaaaagc cgtctgcaag atggacgcgg tcgcgggtac
    15301 gcaataaagg ttccaacagg attttgtcgc ccaatgcagt ttcaaattcg gtcggttcgg
    15361 ttacttccgg attcaggctg cccaagcgca cttcataccc tgccgaacgc aaaatctcgc
    15421 cgagggcgta aatgttttgc aggtaaaacg tattgcgcgt gtggttttcg ggaataatca
    15481 atacggattt cgccgtttcg caggcgcgtt gcaccgcatc ttgcgcggca accgccgcca
    15541 gcgggataaa gttgggattc agattattga agccgccggg gaacaaattc atatcgatag
    15601 acgaaatttt gtaaccggca ttgcgtatat cgaccgaacc gtaaaacggc gggcggtgta
    15661 cgctccactg cgtgcggaac cacgcctcga ttttggcgtg attggacagg atttttgcct
    15721 caaacgcctg aagttgcgcc gaatgttcgg gcgacatcac cggtaatttc atctttcgtc
    15781 tgcctctgtg tgttttttga caagatgcag atgataatgc ctttgaaaga atgacgacaa
    15841 gtattctttc gtatcgaatc atgctttaga tataactgtt tactgaaatg gacaatacgt
    15901 ctaatttttt cgttaaaatt gtttgcaaaa taacaccggc tcaaaaatta gacaaaattt
    15961 gttgcgcggt ataaagaata cgtctaaaat ccgcccaatc cgcattacca tttatccaaa
    16021 agaacaacat catgacagca caaaccctct acgacaaact ttggaacagc cacgtcgtcc
    16081 gcgaagaaga agacggcacc gtcctgctct acatcgaccg ccatttggtg cacgaagtta
    16141 ccagccctca ggcatttgaa ggcttgaaaa tggcggggcg caagctgtgg cgcatcgaca
    16201 gcgtcgtctc caccgccgac cacaacaccc cgaccggcga ttgggacaaa ggcatccaag
    16261 acccgatttc caagctgcaa gtcgatactt tggacaaaaa cattaaagag tttggcgcac
    16321 tcgcctattt tccgtttatg gacaaaggtc agggcatcgt acacgttatg ggccccgaac
    16381 aaggcgcgac cctgcccggt atgaccgtcg tctgcggcga ctcgcacact tccacccacg
    16441 gcgcattcgg cgcactggcg cacggcatcg gcacttccga agtcgagcac acgatggcga
    16501 cccagtgcat taccgcgaaa aaatccaaat ccatgctgat tgccgttgac ggcaaattaa
    16561 aagcgggcgt taccgccaaa gacgtggcac tttacatcat cgggcaaatc ggcacggcgg
    16621 gcggtacagg ctacgccgtc gagtttggcg gcgaagccat ccgcagcctt tctatggaag
    16681 gccgcatgac tttatgcaat atggcgatcg aagccggtgc gcgctcaggc atggttgccg
    16741 tcgaccaaac caccatcgac tatgtaaaag ataaaccctt cgcacccgaa ggcgaagcgt
    16801 gggacaaagc cgtcgagtac tggcgcacgc tggtgtctga cgaaggtgcg gtattcgaca
    16861 aagaataccg tttcaacgcc gaagacatcg aaccgcaagt cacttggggt acctcgcctg
    16921 aaatggtttt agacatcagc agcaaagtgc cgaatcctgc cgaagaaacc gatccggtca
    16981 aacgcagcgg tatggaacgc gcccttgaat acatgggctt gaaagccggt acgccattaa
    17041 acgaaatccc cgtcgacatc gtattcatcg gctcttgcac caacagccgc atcgaagact
    17101 tgcgcgaagc cgccgccatc gccaaagacc gcaaaaaagc cgccaacgta cagcgcgtgt
    17161 taatcgtccc cggctccggt ttggttaaag aacaagccga aaaagaaggc ttggacaaaa
    17221 ttttcattga agccggtttt gaatggcgcg aaccgggctg ttcaatgtgt ctcgccatga
    17281 acgccgaccg cctcgcaccg gggcaacgct gcgcctccac ctccaaccgc aattttgaag
    17341 gccgtcaagg caacggcgga cgtacccacc tcgtcagccc cgctatggca gccgccgccg
    17401 ccgttaccgg ccgctttacc gacatccgca tgatggcgta agtattttca ggcggtcttt
    17461 aagaatttga cgcggccgtc tgaaatatat cccgtaaggc agaaacacct ttaatattca
    17521 aaccaaggag tttaaccatg aacaaacttt tcattaccgc cctgtccgcc ctcgccttgt
    17581 ccgcctgcgc cggcacttgg gagggcgcga aacaagacac cgcccgcaac cttgacaaaa
    17641 cacaggccgc cgccgaacgc gccgccgaac aaacaggcaa cgccgtcgaa aaaggctggg
    17701 acaaaaccaa agaagccgtc aaaaaaggcg gcaatgccgt cggacgcggc atttcccatc
    17761 tcggcggaaa aatcgaaaac gccaccgaat aaccgattcg cgatgccgtc tgaaaccgct
    17821 tcagacggca tcgtcagcac aagacgcaaa cccatgaaag cctttaccaa aattaccgcc
    17881 atcgtcgccc cgctcgaccg cagcaacgtc gataccgacg ccatcatccc caaacaattt
    17941 ttaaaatcca tcaaacgcag cggcttcggc cccaatgcct ttgacgaatg gcgttacctc
    18001 gaccacggcg aaccaggcat ggacaacagc aaacgcccgt tgaaccctga tttctcgctg
    18061 aaccagccgc gttaccaagg cgcgcaaatc ctgttgacgc gtaaaaactt cggttgcggc
    18121 tcttcacgcg aacacgcccc ttgggcattg gacgactacg gcttccgcgc cgtcatcgcc
    18181 cccagcttcg ccgacatctt ctttaacaac tgctacaaaa acggcctttt acctatcgtg
    18241 ttgaccgaag aacgggttga ccgacttttc aaagaagttg aagccaacga aggctatcag
    18301 ctctccatcg accttgccga acaaaccctg accacaccaa gcggcgaaac gttcacattt
    18361 gacattaccg aacaccgcaa acactgcctc ttaaacggct tggacgaaat cggtctgacc
    18421 ctgcaacacg ccgacgaaat tcacgccttt gaagaaaaac gccgccaaag ccagccttgg
    18481 ctgtttaacg gttaaaacct ataccgtctg aaggccgttc agacggcatt cctgataaca
    18541 tataattgtt gcggctaatg taaacagagg ggcgataaat tgctttcatc ttttaagcgg
    18601 gcttagcgac actctaaatt aatgcaataa tgcaactctc catttcaaat aaatttttag
    18661 atttttaata tgcaacaaat taaatttatt gacttatttt ccgaaatgag tggcatcagg
    18721 aaaggattcg aacaagcctg tcggaaacag tcggttgctt gcgaatgtgt ttttacttca
    18781 gaaatcaaac cggcagcttt ggaagtattg aagcaaaact accctgatga agtgccgtat
    18841 ggagatataa cgaaaattga aacaggggat attcccgatt ttgacatcct gttggcaggc
    18901 ttcccttgtc aggctttttc tttcgcctga aaaagattag gctttgaaga tacacgggga
    18961 acgcttttct ttgatgtggc aaggatttta aaggcaaaaa aaccaaaagg ttttattttg
    19021 gaaaatgtgg aaggattggt gacacacgat agaaaagatt cgacacaaaa aatagggcgc
    19081 accctgaccg ttattttgga aaccttggaa gcattgggct actatgtttc ttggaaagtt
    19141 ttaaatgcaa aagatttcgg cattccccaa aacaggaagc gcatttatct gacaggcagt
    19201 ttgaaatcca aacccgactt atctttcgaa acaagcccaa gtccgaaatt aaaaaatatt
    19261 ttggaatcgg gactgcctac cgaaagcagc cctttcatca aaaaattgct aaaaaaattt
    19321 cccccgtccg aactgtacgg aaaatcagta aaagacaaac ggggagggaa aaacaatatt
    19381 cacagttggg atattgaatt aaaaggcgca gtaaccgaag aagagaagca attgttaaat
    19441 atccttctaa aagaacggag gaaaaaaaat gggcttcaga aatcggcata gattggatgg
    19501 atgggatgcc tttgacaaaa gcgcaaattt caactttcta taaacacccc gatctccaaa
    19561 atattttgga cagcctgaca gataaaggct atttggtttt agagcatccg aaacaaaaaa
    19621 ttggcgggca aagaatcaaa gatgaatccc tgcccaaagg ctacaatatt gtttcaggca
    19681 aaaaatcttt tgaaatcaat aaaatattag atcaaaacga tgttgcgcca accttggttg
    19741 caatgaatat ggagcacctt ttcgtcgttg acaacggcgg tttgcgtaca cttaccggaa
    19801 aagaagggtt gcgcttattc ggctatccgg acgattattc gtttgatatt cccaaaaaag
    19861 acagatgcga tttattgggt aataccgttg ccgtccctgt gattaaggcg gtatctgaaa
    19921 gacttctgca tactttataa accgacaggg ataaaatgat aaaacttact gcacaacaaa
    19981 tatttgataa gttgctggat gaagaaaaaa tcttatcggc caatggtcaa attagatttt
    20041 tcttaggcga tgtggatatt atcgtcaaac aaaaagatgt tgtcggcaac atcattcagg
    20101 aatggctcgg cggatgattg agaaaaaggg aaattgaatt tgatgtttca accaataccc
    20161 aaatgcctcc cgatttcttt ttaaataaaa aagaccgcag cagagaattg ttggaagtaa
    20221 aggcgttcaa ccgaaatgcc ggcccgggtt ttgatattgc agattttaaa atgtattctg
    20281 ataaaatcat tcataagccc tatatgctgg atgtagacta tttaatattc ggttacgata
    20341 tggacgacaa cggcaatgta accatcaagg atttatggct taaaaaagta tggcaaatta
    20401 cgagaagcat ggatgggtgg gcaattaatc ttcaagtcaa aaaaggcgtg gtgcataaaa
    20461 tccgcctggg tgtttggtac agcataaata aaaagaatat gcctatgttt gaatgcttgg
    20521 aagattttgt ttccgcaatt gaagaaaccg tttatcaaaa cccggcaaca cggcataacg
    20581 catccttatg gaaaaagaaa tttgaggaag cctataaaaa acactacaac cgatcgattt
    20641 ccatacctcg ttggcacgaa attgcacaca aatacaaaaa gaaataaccc ttcaaccatc
    20701 aacccataag gaaacttgaa atgaccaaac atatcgccat cctccgaggt gacggcatcg
    20761 gtcccgaaat cgtcgccgaa accgtccgcg tactcgacaa actcatcgcc caaggtttgg
    20821 atgccggcta cgaatacgcc ccattgggcg gcgaagccta tgacgaatac ggtcatcctt
    20881 atcccgaatt tacgcaaaac ctctgccgca aagccgatgc cgtcctgctt ggtgcagtcg
    20941 gttctcctca atacgacaac ctcgaccgtc cgttgcgccc tgagcgcggc ctgttggcaa
    21001 tccgtaagga tttgaacctg tttgccaatt tgcgtcctgc tgttttatat cccgaattag
    21061 ccaacgcctc cacgctgaag cctgaaatcg ttgctggttt gaatattttg attgtgcgcg
    21121 aactcaccgg cgacatctat ttcggcgaac cgcgcggcat ccgcgttctg gaaaacggcg
    21181 aacgcgaagg ctacaacacc atgaaataca gcgaaagcga aatccgccgc atcgcccacg
    21241 tcgctttcca atccgcccaa aaacgcagca aaaaagtctg ctccgtaggc aaagccaacg
    21301 ttttggaaac caccgaactg tggcgcgaaa tctttgaaga aatcggcaaa caatatcccg
    21361 atgtcgaact ttcccatatg tatgtggaca acgccgctat gcagctcgtg cgcgcgccca
    21421 aacagtttga cgtgattgcc accggcaaca tcttcggcga catcctctcc gacgaagcct
    21481 ccatgctgac cggctccatc ggtatgctgc cttccgcttc tttggacgaa aacggcaaag
    21541 gcctgtacga accgtcccac ggctccgctc ccgacatcgc cggacaaaac aaagccaacc
    21601 cgctggccac catcctctcg ctcgcaatgt tgctgcgtta cagcctgaac gacgaagcgc
    21661 gcgcgcaaca agttgaaaac gccgtccaaa aagtgctgca acaaggcttg cgtaccagcg
    21721 acatttacga agaaggcaca aaactcgttt cctgctccga aatgggcgat gcggtactcg
    21781 ccgccttgta aaaaaacaaa gcgcggatca caccgcgctt tttcatctgc ccttttccgc
    21841 tcgccccgtt cgtcaaccgc ctgcaaagcc accgtttacc ggcaggaaat gccataaatc
    21901 cccgcctatg ttaggataaa cgcctttact ggctcacaca aaggattatc atcatgaaaa
    21961 aaatcatctt cgccgcactc gcagcggcag ccgttggcac tgcctccgcc gccacctaca
    22021 aagtggacga atatcacgcc aacgcccgtt tcgccatcga ccatttcaac accagcacca
    22081 acgtcggcgg tttttacggt ctgaccggtt ccgtcgagtt cgaccaagca aaacgcgacg
    22141 gtaaaatcga catcaccatc cccgttgcca acctgcaaag cggttcgcaa cactttaccg
    22201 accacctgaa atcagccgac atcttcgatg ccgcccaata tccggacatc cgctttgttt
    22261 ccaccaaatt caacttcaac ggcaaaaaac tagtttccgt tgacggcaac ctgaccatgc
    22321 acggcaaaac cgcccccgtc aaactcaaag ccgaaaaatt caactgctac caaagcccga
    22381 tggcgaaaac cgaagtttgc ggcggcgact tcagcaccac catcgaccgc accaaatggg
    22441 gcgtggacta cctcgttaac gttggtatga ccaaaagcgt ccgcatcgac atccaaatcg
    22501 aggcagccaa acaataaatc ttgatgcgaa tcagcacaaa tgccgtcttc atcaaaccgc
    22561 cccgccaacg gtaagccgat atggcggagc acaatccgcc gaaagatatt tcagcggcag
    22621 gcagggcttg ccggaaagtc cggaaagtat agtggattaa caaaaaccag tacggcgtt
//
LOCUS       DAXHNE010000016        21858 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.016, whole genome shotgun sequence.
ACCESSION   DAXHNE010000016 DAXHNE010000000
VERSION     DAXHNE010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21858
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.016"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..1169)
                     /gene="hemW"
                     /locus_tag="ACFM4Z_000480"
     CDS             complement(<1..1169)
                     /gene="hemW"
                     /locus_tag="ACFM4Z_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFC6020571.1"
                     /translation="MHAISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETVFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     ATALKLFDKINIDLMYALPNQTVRTALDDVQTAIATGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDTVHGALENAGFIHYETSAFAKPAMRCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQNRPSEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPAAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLNDLLQC"
     gene            complement(1226..3751)
                     /gene="ligA"
                     /locus_tag="ACFM4Z_000481"
     CDS             complement(1226..3751)
                     /gene="ligA"
                     /locus_tag="ACFM4Z_000481"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFC6020572.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPEFKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVANIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQGGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEQMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAIAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMQETAVAVSDGIGHRQDDLFAET
                     PSANQTQSVPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETTDKTVAESDQTPSEG
                     SSVGASGKHKKQPVKWAENILGGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVAHSIAHFFAQAEQQAMIDELLAAGVAPQTQAVTIPP
                     ARHAEPQRWIARLPDFKISENKAQALWELAGKNIEGLQNDKALPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSILSEAELLTLLG"
     gene            complement(3899..5185)
                     /locus_tag="ACFM4Z_000482"
     CDS             complement(3899..5185)
                     /locus_tag="ACFM4Z_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HFC6020573.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGQPSGGPVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPAPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            complement(5379..5951)
                     /gene="ampD"
                     /locus_tag="ACFM4Z_000483"
     CDS             complement(5379..5951)
                     /gene="ampD"
                     /locus_tag="ACFM4Z_000483"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFC6020574.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFGGREKCNAFSIGIELEGCDFEPFAEAQYRSLEALLDAICRRYPVTAVTGHQD
                     IAPGRKTDPGHFFDWRRIREKGFPVDRNAV"
     gene            6035..7030
                     /gene="mltG"
                     /locus_tag="ACFM4Z_000484"
     CDS             6035..7030
                     /gene="mltG"
                     /locus_tag="ACFM4Z_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFC6020575.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQIAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            7090..7710
                     /gene="tmk"
                     /locus_tag="ACFM4Z_000485"
     CDS             7090..7710
                     /gene="tmk"
                     /locus_tag="ACFM4Z_000485"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFC6020576.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQTREKDRFEQEQAD
                     FFMRVRSVYLDRAAACPERYAVIDSNLGLDEVRNSIEKVLDRHFGC"
     gene            7932..9212
                     /locus_tag="ACFM4Z_000486"
     CDS             7932..9212
                     /locus_tag="ACFM4Z_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HFC6020577.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            9448..9651
                     /locus_tag="ACFM4Z_000487"
     CDS             9448..9651
                     /locus_tag="ACFM4Z_000487"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020578.1"
                     /translation="MKEMKPVRYHIGDMPETSKQTASRHDDRAVGVDDDCFHDSFGQF
                     LFGCSAEATRTAPISVKRSCLFT"
     gene            9759..10793
                     /gene="lpxK"
                     /locus_tag="ACFM4Z_000488"
     CDS             9759..10793
                     /gene="lpxK"
                     /locus_tag="ACFM4Z_000488"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFC6020579.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAASRAEDAGDEPLLLFSKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            10999..11580
                     /locus_tag="ACFM4Z_000489"
     CDS             10999..11580
                     /locus_tag="ACFM4Z_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HFC6020580.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            11649..11831
                     /locus_tag="ACFM4Z_000490"
     CDS             11649..11831
                     /locus_tag="ACFM4Z_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HFC6020581.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARPLGEEELEA"
     gene            11828..12589
                     /gene="kdsB"
                     /locus_tag="ACFM4Z_000491"
     CDS             11828..12589
                     /gene="kdsB"
                     /locus_tag="ACFM4Z_000491"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFC6020582.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAV
                     RVVVATDHPDIQTACQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPFETAVLRHIGIYAYRVGFLQRYTEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRIRAVFQTI"
     gene            12612..12929
                     /locus_tag="ACFM4Z_000492"
                     /pseudo
     CDS             12612..12929
                     /locus_tag="ACFM4Z_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            13031..13231
                     /locus_tag="ACFM4Z_000493"
                     /pseudo
     CDS             13031..13231
                     /locus_tag="ACFM4Z_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            13388..13480
                     /locus_tag="ACFM4Z_000494"
     tRNA            13388..13480
                     /locus_tag="ACFM4Z_000494"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13422..13424,aa:Ser,seq:gct)
     gene            13783..14568
                     /gene="trpA"
                     /locus_tag="ACFM4Z_000495"
     CDS             13783..14568
                     /gene="trpA"
                     /locus_tag="ACFM4Z_000495"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFC6020583.1"
                     /translation="MSRIRQAFAALNGEKALIPYITVGDPDIGATLAIMHSMVENGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAMAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAVVGALVKELKDAVR"
     gene            14605..15477
                     /gene="accD"
                     /locus_tag="ACFM4Z_000496"
     CDS             14605..15477
                     /gene="accD"
                     /locus_tag="ACFM4Z_000496"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFC6020584.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPIDPLKFKDSKKYPDRLGAA
                     RKLTGEDDALVVMKGMMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(15537..16148)
                     /locus_tag="ACFM4Z_000497"
     CDS             complement(15537..16148)
                     /locus_tag="ACFM4Z_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HFC6020585.1"
                     /translation="MRRTALLLLALAAGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSMKPHNK"
     gene            16257..16481
                     /locus_tag="ACFM4Z_000498"
     CDS             16257..16481
                     /locus_tag="ACFM4Z_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HFC6020586.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVQHK"
     gene            16482..17087
                     /locus_tag="ACFM4Z_000499"
     CDS             16482..17087
                     /locus_tag="ACFM4Z_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020587.1"
                     /translation="MPSEMRMSRLMLLLSAFVLAACNAQNRQSGVRHQDFARVAVDLF
                     RSACVLTQGSFESVSKFAAVGHFVPVGKEDLSHLPPTVAEPDVQALWTLERDGGIYYL
                     SLTRDSCSVKAERADSAALLEHFDGLVRQPPKNANIELRAEQSVQASFEIRQVSYAWR
                     EAGSSEETVLTAQTADSPDLPVQAVLNLTHRSHNGKPLILP"
     gene            17147..18181
                     /gene="pyrC"
                     /locus_tag="ACFM4Z_000500"
     CDS             17147..18181
                     /gene="pyrC"
                     /locus_tag="ACFM4Z_000500"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFC6020588.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDQATPALVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAVELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENAGTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(18549..18974)
                     /gene="nusB"
                     /locus_tag="ACFM4Z_000501"
     CDS             complement(18549..18974)
                     /gene="nusB"
                     /locus_tag="ACFM4Z_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFC6020589.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(19052..19528)
                     /gene="ribH"
                     /locus_tag="ACFM4Z_000502"
     CDS             complement(19052..19528)
                     /gene="ribH"
                     /locus_tag="ACFM4Z_000502"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFC6020590.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNLASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(19576..19899)
                     /locus_tag="ACFM4Z_000503"
     CDS             complement(19576..19899)
                     /locus_tag="ACFM4Z_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFC6020591.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADIAKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            20066..20785
                     /gene="rnc"
                     /locus_tag="ACFM4Z_000504"
     CDS             20066..20785
                     /gene="rnc"
                     /locus_tag="ACFM4Z_000504"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFC6020592.1"
                     /translation="MKDDVLKQQAHAAIQKRLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDTFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRCADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVVSCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            20832..21755
                     /gene="era"
                     /locus_tag="ACFM4Z_000505"
     CDS             20832..21755
                     /gene="era"
                     /locus_tag="ACFM4Z_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFC6020593.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
BASE COUNT         4825 a         5348 c         6499 g         5186 t
ORIGIN      
        1 aaacactgca gcaaatcgtt taaaaacaag cgtccttttt cggtcgggcg gaatacggca
       61 gggtcggttt ccagcaggcc tttttgcctt gccgtttcga tttgcgccat gattttggcg
      121 gcgggtacgc ccgtgcgttc ctgcaacatc gcggcgggta cgccgtcggt caggcgcagg
      181 gcgttcatca taaactcgaa aggcaaatct tcggcggcga cggttttgcg ttcgacggct
      241 tcactcggcc ggttttgcat taaggcgagg tagtcgttgg gatggcggcg gcggacggtg
      301 cgctcgatgc ggtcggggta ggaaattttg ccgtgcgcgc ccgcgcctat gcccaaataa
      361 tcgccgaact gccagtagtt caaattgtgg cggcaccgca tggcgggttt cgcaaaagcc
      421 gatgtttcgt aatgtataaa gccggcgttt tccaatgcgc cgtgtaccgt gtcttcgatg
      481 tcgagggcgg cttcgtcttg cggcaaacct ttcggcggcg tgtgtccgaa cggcgtgttc
      541 ggttccatcg tcaggtgata cgcgctgatg tgggttgcgc ccgtggcgat ggcggtttgt
      601 acgtcatcca atgccgtccg aacggtttgg ttcggcaggg cgtacatcaa gtcgatattg
      661 attttatcaa ataatttcaa ggcagtagct atggcggtta aggcttcttt accgttgtgg
      721 acgcgcccca gccttgagag catatcgtcg ttgaagcttt gtacaccaat agaaagccgc
      781 gtgatgcctg cgtctttaaa tccttgaaac ttctcgattt caaatgtccc cggattagct
      841 tccaacgtaa tttccgcttc cggctgtaag cgcaacagcg aacgcacgcc gctcaacaaa
      901 cggtcgatcg attccgcctg aaacaggctg ggcgtaccgc cgccgaaaaa taccgtttcc
      961 accggcctgc cccaaatatt gggcaattca agctgcaagt cggtcagcag cgcgtcgata
     1021 taggcggctt cgggcaatcc gtttttcagg ctgtgggaat tgaagtcgca atacgggcat
     1081 tttttgatgc accacgggat gtggatatag agcgacaggg gcggcagggc ggtgagccgg
     1141 gtgcggtttg gaaaggaaat ggcgtgcatg gtgtggttcg gaaaagtgga caatgccgtc
     1201 tgaaggcggt tcagacggca caggttcagc cgagcagggt aagcagttcg gcttcgctga
     1261 ggatggaaac acccaaggca ttggcttttt ccagcttgct gcccgcggct tctccggcga
     1321 cgacgtagtc ggtttttttg gacacgctgc cggaaacttt gccgcctgcg gcttcgatta
     1381 gggattgggc ttggtcgcgt ttgagcgtgg gcagggtgcc ggttaataca aaggttttgc
     1441 ctgctacggc tttattgatg ccgtctgaac cttgcgccgc ctcgtcttca gacggcattt
     1501 gcgcgaagaa ggttttcagg ttttcgagta gggcggcgtt ttgtggttcg ctgcgccatg
     1561 cttgccagtc ggcggggagg gctttgtcgt tttgtagacc ttctatgttt ttaccagcga
     1621 gttcccataa ggcttgggct ttgttttcgc taattttgaa gtcgggcagg cgggcgatcc
     1681 agcgttgcgg ttcggcgtgg cgggcgggcg ggatggtaac ggcttgggtt tgcggggcaa
     1741 cgcctgcggc gagcagttcg tctatcatcg cctgctgttc ggcttgggcg aagaagtggg
     1801 caatggaatg cgccactacc gtaccgatgt cgggcaggca ggcgaggacg ggttcggggg
     1861 cgcggcggac gcgttccaat gtgccgaatg cctgcgccag cgttttggcg gtgcgttcgc
     1921 cgacgtggcg gatgccgagc gcgaacagga agcgcgcgag ttcgggcgtt ttgccggatt
     1981 ctatgccccc gaggatgttt tccgcccact tgaccggttg ttttttatgt ttgcccgacg
     2041 cgccgaccga actgccttca gacggcgttt gatccgattc ggcaacggtt ttgtccgtcg
     2101 tttctttcat tttttgcagg gtcggaatat cgaggcggta gaggtcggcg aagtggcgga
     2161 cgaggtcttg ggcgacaagc tgttcgattt gtttttcacc caagccgtcg atgtccatgg
     2221 ctttgcgcga ggcgaagtgg attaagcctt gcgcgcgttg tgcctgacaa agcataccgc
     2281 cgctgcatcg ggcgacggct tcgccttctt cgcgttcgat ttcgctgcgg cagatggggc
     2341 aatgggtcgg caggcggtag ggcttgtgga gcggaacgga ttgggtttga ttagcggacg
     2401 gtgtttcggc aaacaaatcg tcctgccgat gcccgatgcc gtctgaaaca gcaacggcgg
     2461 tttcctgcat cgggcggcgt tcaaaaatca cacgcacgac ttcgggaatc acgtctccag
     2521 cgcggcgcac gacgacggta tcgccgacgc gcacgtcttt tcgggaaact tcgtcctgat
     2581 tgtgcagggt ggcgttggta acggttacgc cgccgacgaa tacgggttgt aatcgggcaa
     2641 ccggcgttac cgcgcccgtc ctgccgattt gcacgtcaat cgcttcgaca atggtcaggg
     2701 cttcttcggc agggaatttg tgggcaatcg cccagcgtgg cgcgcgtgag atgaagccga
     2761 gttcgcgctg ttgcgccaag ctgttgactt tgaccaccat accgtcgatt tcgtagggca
     2821 gttcggggcg tttttgctgc atttgttcgt aaaacgccaa tacttcgtcg atatttttga
     2881 aacagccgaa attgccattt ggcagactga agccgagtgc ttggaaatag gcgagttctt
     2941 ggatgtgttc ttccgcgacg aaaccgcctt gctggcgggc gacggagtag gggaaaaagt
     3001 gcagtttgcg ttgcgcagtg atgcgcgaat cgagttggcg caggctgccg gcagcggcgt
     3061 tgcgcggatt ggcaaagggt ttttgcccgt tttcggcttg tcttttattg agggcggcaa
     3121 aatcggcttt ttgcatcagt acttcgccgc gtacttcgat gagttcgggc gtattttcgc
     3181 cgtgcagccg cagggggatg ttggcgacgg ttttgatgtt ttgggtaacg tcttcgcccg
     3241 ttgtgccgtc gccgcgcgtt gccgcctgta ccaatacgcc gtcgcggtag agcaggctga
     3301 tggcgaggcc gtcgaatttg ggttcgataa cgtattcggg attgccgccg tccaaaccgt
     3361 cgcgcacgcg ttggtcgaaa gcgtacattt cggcgtggtc gaacacgccg ttttcatctt
     3421 gcggggaaaa ggcgttggtc agcgacagca tcggcacttc gtggcgtact tcggcaaatc
     3481 ccgccagagg ctcgccgccg acgcgctggg tcgggctgtc gggcagtttg aattcgggat
     3541 ggtttaattc caacgcttcg agctcgcgga ataatttgtc gtattcggca tcgggtacgc
     3601 tgggcgcgtc gagggtgtag tattcgtagg catagcggtt gagcaggtcg gtgaggtggc
     3661 ggatgtgttg tgcggcaaat tgttttatat cactatcaga cggtttaaga agattggtaa
     3721 agttagtgtt atgttttgag tttggattca tgagagaagg ttttcagacg acctttgtct
     3781 gatacgggat gaaacgggca aaggtcgtct gaaaaatgat aggttgaaaa cagctgaatt
     3841 ttacccgaaa aaaagcggat atgccgtaac gacatatccg ctttgattgc attcgatttt
     3901 aggagaacag gcgcaatgcg gttttgccgc ccggttcgat accgactttg agcatctcgg
     3961 actgacgagc caatacataa gtgcgtacgt ctttgagcca ttgggtcgaa acttcttcca
     4021 ttttgtcgtt gaccagattc aggttcaact ggccggacag gcgtaccgcc aaatccataa
     4081 acaaatcgtc gaaggttttt tcgcctgccg gagagtgcgg gatgtcgagc agcatactga
     4141 agcctttgta ggactggttg tccaaaaggg cattggtaaa cggctcgttg ttgagcgagc
     4201 agatggagaa catggttgag cccgacgtgt cggtatagtg gaacgcgccg tcgtcttcca
     4261 aaacgaaacc cacgcccgtt acggcggaac gcagttctac gccgctgatg ctggtcgggg
     4321 aaaccaaatg gatggcgatg gtctggtcga cgcgcgcgca gaatgcgtcc agtgcggaag
     4381 ccacttcgat aaaggcggca aggtcggtgt gcagcgtctg accgcccatg ctttgtgcga
     4441 atgcgtccac ctggcggttg aatgcggaga gttcttcctg cgaggcaagt ccgttgcggc
     4501 tgactgcctg aatacccacg ataaatgcct gatagcggat gcccgggatg ggttcggcaa
     4561 tctggaaatg gtcgtccatg gtgcagccga caatctggta gcggcagcgg ttggaaaggc
     4621 gcggcagtgc gtgcagttct ttggcttcgg tcagcgcgat ataggagatg aagtcgaagc
     4681 gcacgtcaaa ccagggtaat tcgacttttg acagttcttt gagcgtaatc agcggttttg
     4741 caggtgtttg cggaacgggt gcaggttttg ccggcgcgtc ggcaggtttc ggtgcggaat
     4801 gtccggtttg gggttcggaa acggtgtggg cggagttgcc gataatgccg ctttcttcca
     4861 aggcggtttc gatttcggtt ttgaacgggg aggcttttgc ctgtttctgc ttggcgatgt
     4921 agacggcatc ctgttcttgc aggttgcgca tggcggggtc ttggggtttt gccgtttttt
     4981 tgaccgccgg ttggggtttc ggcatcatga ctggcccgcc ggacggttgg ccgtcgcgga
     5041 catggctggt tttgctgttg agcagggcat ctttgtcgga gtgtccgaac tggtcgcgca
     5101 cttttttgcg gtattggttt tcctgataca tattgtaggc gacaacggcg aggacggcgg
     5161 cgaggaacag tacgatgtaa atcatggcaa tcactttaaa tttcgggatg caggatgcgc
     5221 gaagtgcggg tactgcggtt aaatcgggct tgcgtttccg gcagtctgat ggaacggccg
     5281 gttataacgt ttgaattata acgaaaattg cagggtctga cagcagtgtg tcgaaataag
     5341 cggaaatttt ccgaaatgcc gtctaaaatc tgtggttttc agacggcatt tctgtctacg
     5401 ggaaaccctt tctcccgtat ccgccgccag tcgaaaaagt ggccggggtc ggttttgcgg
     5461 ccgggcgcga tgtcctgatg tccggttact gcggtaacgg ggtagcggcg gcagattgcg
     5521 tccaacaagg cttcgagcga acggtattgc gcttcggcaa acggttcgaa atcgcagcct
     5581 tccagttcga tgccgattga aaatgcgttg catttttccc tgccgccgaa tgaagatacg
     5641 ccggcatggt atgccatatt gtcgcaggaa acgaactgta ccgtttctcc gtcgcgtttg
     5701 attaagaaat ggctggatac gcgcaaagtg tgtatcaggc tgaagaacgg atgtccgtcg
     5761 gggtcgagcc ggttggcaaa cagcttttcc accgcatctg tgccgtattc gaacggcggc
     5821 agcgaaatgt tgtgcaacac gatcagggaa accgtttccc ccgtttccct cgggctgaaa
     5881 ttgggcgacg gggtatggcg tatgctttga agccagccgt tttgccagtg tgcttcggcg
     5941 tgattgtcca tgatgttctt cctgtccggc gggtaatttg ggttatactg tcgcccgaat
     6001 tttaagacgt attccgaatg ctgggaatcc taccatgttg agaaaattgt tgaaatggtc
     6061 tgccgttttt ttgaccgtat cggcagccgt tttcgccgcg ctgcttttcg tccctaaaga
     6121 caacggcagg gcatacagga ttaaaattgc caaaaaccag ggtatttcgt cggtcggcag
     6181 gaaacttgcc gaagaccgca tcgtgttcag caggcatgtt ttgacggcgg cggcctacgt
     6241 tttgggtgtg cacaacaggc tgcatacggg gacgtacaga ctgccttcgg aagtgtctgc
     6301 ttgggatatc ttgcagaaaa tgcgcggcgg caggccggat tccgttaccg tgcagattat
     6361 cgaaggttcg cgtttttcgc atatgaggaa agtcatcgac gcaacgcccg acatcgaaca
     6421 cgacaccaaa ggctggagca atgaaaaact gatggcggaa gttgcccctg atgccttcag
     6481 cggcaatcct gaagggcagt ttttccccga cagctacgaa atcgatgcgg gcggcagcga
     6541 tttacggatt taccaaatcg cctacaaggc gatgcaacgc cgactgaatg aggcatggga
     6601 aagcaggcag gacgggctgc cttataaaaa cccttatgaa atgctgatta tggcgagcct
     6661 gatcgaaaag gaaacagggc atgaagccga ccgcgaccat gtcgcttccg tcttcgtcaa
     6721 ccgcctgaaa atcggtatgc gtctgcaaac cgacccgtcc gtgatttacg gcatgggtgc
     6781 ggcatacaag ggcaaaatcc gtaaagccga cctgcgccgc gacacgccgt acaacaccta
     6841 cacgcgcggc ggtctgccgc caaccccgat cgcgctgccc ggcaaggcgg cactcgatgc
     6901 cgccgcccat ccgtccggtg aaaaatacct gtatttcgtg tccaaaatgg acggtacggg
     6961 cttgagccag ttcagccatg atttgaccga acacaacgcc gccgttcgca aatatatttt
     7021 gaaaaaataa accatgccgt ctgaaaagtt tgtgttttcg gacggcatac ccttaccgga
     7081 actgcaagca tgaaaccgca attcatcact ttggacggca tagacggtgc cggcaaatcc
     7141 accaaccttg ccgtcatcaa ggcatggttt gaacggaggg ggctgcccgt gctgttcacg
     7201 cgcgaaccgg gcggaacgcc ggtcggtgag gccttgcgcg aaatcctgct caaccctgaa
     7261 accaaagccg gtttgcgtgc ggaaacactg atgatgttcg ccgccagaat gcagcatatt
     7321 gaagacgtga tattgcctgc actttcagac ggcatccatg tcgtgtccga ccgttttacc
     7381 gatgcgacct tcgcctatca gggcggcggg cggggaatgc cgtctgaaga cattgaaatt
     7441 ttggaacatt gggtgcaggg cggtttgcgc cccgatttga ccctgctgtt ggatgtgccg
     7501 ctggaagtgt cgatggcgcg tatcgggcag acgcgcgaga aagaccggtt cgagcaggaa
     7561 caggcagatt tcttcatgcg tgtgcgcagc gtttatctcg accgagccgc cgcctgtccg
     7621 gaacgttacg ccgttatcga cagtaacctc ggcttggatg aagtcagaaa cagcatagaa
     7681 aaagtgttgg acagacattt cggctgttga tgcggcaaat attgaaacaa gcgcatccgc
     7741 ccgcgccgaa aatcaaacgg cagtgccgca ggtgaaaatg gcggtatgcg ccaaactttc
     7801 ggcatgatag aattacgctc ggttacaagg caggatgcgt cggcaatatt aacgaaccgc
     7861 ccgtaacatg atgacccgaa agcgtttcgg acagtccgat tcaaatcttt ttctcgcaac
     7921 aggattgaca catggaaaac tcattgaaag aagccgccct caagttccac gaattacccg
     7981 ttccgggcaa aatttccgtt accccgacca aatctctggc gaccgacaaa gatttggcgt
     8041 tggcgtactc tccgggcgta gccgctcctt gtatggaaat ccatgccgat ccgcaaaatg
     8101 cctacaaata caccgccaaa ggcaacttgg tcgctgtcat ttccaacggt acggccgttt
     8161 tgggcttggg cgacatcggc gcgctggcgg gcaaacccgt gatggaaggc aaaggcgtat
     8221 tgttcaaaaa attcgccggt gtggacgtgt tcgacatcga aatcgatgaa aaagacccgc
     8281 aaaaactcgt ggacatcatc gccgctttag agccgacctt cggcggcatc aacctcgaag
     8341 acatcaaagc acccgagtgt ttctacatcg aacgcgaatt acgcaaacgc tgcaaaatcc
     8401 ccgtattcca cgacgaccag cacggcacgg ccatcattac cgccgccgcc gtattgaacg
     8461 ctctgcgttt taccggccgt aaaatcgaag aagcgacctt ggtttgctct ggtgcgggtg
     8521 cggccgcgat tgcctgcttg aaccaattgc tggatttggg cttgaaacgc gaaaacgtaa
     8581 ccgtttgcga ctccaaaggc gtgatttacc aaacccgcga agacaaagat cgcatggacg
     8641 aatccaaaaa attctacgcc gttgaagaca atggccagcg cgtacttgcc gatgccgtca
     8701 aaggtaaaga catcttcttg ggcctctccg gcgcgaacct gctgaccccc gaaatgttga
     8761 acacgatgaa cgaaaaaccc atcgtgttcg ccatggccaa cccgaatccg gaaatcctgc
     8821 cgccgctggc aaaagaaacc cgtccggacg tggttatcgg taccggccgt tccgacttcc
     8881 cgaaccaagt caacaatgta ttgtgcttcc cgttcatctt ccgcggcgcg ttggatgtcg
     8941 gcgcgaccac catcaacgaa gaaatgaaac gcgcctgcgt gtatgctttg gcggatttgg
     9001 cgatggaaga agtaaccgaa gaagtggttg ccgcttacgg taagaaattt gaattcggcg
     9061 cggaatacct gattccgact ccgttcgatt cccgcctgct gccgcgcgtt gctacggctg
     9121 ccgccaaagc agcgatggaa agcggagtgg caacccgtcc gattgcagat ttggaagctt
     9181 acgctgccaa gctgagcgaa tggaagctgt aagccgtttg cggtttaaaa tgccgtctga
     9241 actgttttca ggcggcattt tgctgtcaga ttgatatagt ggattaacaa aaatcaggac
     9301 aaagcgacga agccgcagac agtacaaata gtacggaacc gattcacttg gtgtttcagc
     9361 accttagaga atcgttctct ttgagctaag gcgaggcaac gccgtactgg tttttgttaa
     9421 tccactataa atgaaagata ctggaaaatg aaagagatga aacctgtccg ttatcatatt
     9481 ggcgatatgc ccgagacttc aaaacaaacc gcctcccgtc atgacgacag ggcagtgggt
     9541 gttgacgatg attgttttca tgattccttt ggtcaatttt tgtttgggtg ttcggcagag
     9601 gcaacccgaa ccgcgccaat ttctgtaaag cgcagttgct tatttacctg attggttcgc
     9661 ttatcggttt ggtcttcgcg ttgtttatag gtgggtctgt atcaggtacg catgattaat
     9721 gccccgggct gattttgctt cgaggatttg tatcgaatat gccgaattgt ttcaaatttc
     9781 ataccgttat cgaacggcat tggcaaaaac cttatccggt tttgtctttt ctgcttaagc
     9841 cgctctccgg gctgtttgcc aaaattgcgg caaaacggcg gacggatttt ttatcgggaa
     9901 aacggcaaag cgaaaagctg cccgtgcctg tggtcgtggt cggcaacatc cacgcgggcg
     9961 ggacggggaa aacgccgatt gttgccgcgc tggtgtcggg tttgcaggaa aagggcgtca
    10021 aggtcggcat catcagccgg ggctacgggc gtaagagcaa ggcggttcat gtattgaatg
    10081 ccgcaagccg agcggaagat gcgggcgacg agcctttgct gctgttcagc aaaaccggtg
    10141 cgccgacggc ggtgggcagc agccgtgcag aggcaggcag ggcgttgctg gcggcgcatc
    10201 ccgacatcgg actgattgtg gcggacgacg gtttgcagca ttacgccctg cggcgagatg
    10261 tggaaatcgc ggtgtttccg gcggcggata cggggcgcac ggatttggat ttactgccca
    10321 acggcagttt gcgcgaacct ttgttgcggc tggattcggt ggatgcggtc gtcgtcagcg
    10381 gcggcaaggc ggatgcgctg tttaggccgt ctgaaaatat gtttcacagc cgtatcgaag
    10441 cgggacggat ttaccgtttg aacaatccgt ccgaaatact ggacacaggc cgtctgaaaa
    10501 atcaaaccgt cgtcgccgtg gcaggtattg ccaagccggc gcgttttttc gattcgttgc
    10561 ggaatatggg cattaccgtg aagcgaaccg tcgcgctgcc cgaccacgcc gacatttcgg
    10621 cggcagattt gcccgatgcg gacgcggtca ttattacgga gaaagatgcg gtcaaatttt
    10681 cagacggcat ttgcaccgat aatgtttggg tgttgcccgt ttgtgcgata atcgaacctg
    10741 atttggcggc gtttgtgttg gagcggttgg aagatgtacc gaaggccgtc tgaaagcacg
    10801 gtttgggcgg agtgattacg gatttgaata agaacgcctc gcgccgtcat tcccgcgcag
    10861 gcgggaatct aagtctcgaa ttttcaggaa tgcctaggag gcttcagaaa tcccaaatct
    10921 ccggatttcc acttggacag gaatgagaaa atcggtcgta ttttttatct gcattaatca
    10981 ttcgttaaag tattgaatat taaactgaaa accttgttat tgcccttcgc cacgctggca
    11041 ttgtgcacca acgcttttgc cgccccgcct agcgacgcgt cgttggcgcg ttggctggat
    11101 acgcagaatt ttgaccggga tatagaaaaa aatatgattg agggctttaa tgccggattt
    11161 aaaccgtatg cggacaaagc ccttgccgaa atgccggaag cgaaaaaaga tcaggcggca
    11221 gaagccttta accgttatcg tgagaatgtt ttgaaagatt tgattacgcc cgaagtgaaa
    11281 caggctgtcc gtaatacttt attgaagaat gcccgtgaga tatacacgca agaagaaatt
    11341 gacggcatga ttgcctttta cggttcgcct gtcggtcagt ccgtcgttgc caaaaatccg
    11401 cgcttaatca agaaatcgat gagtgaaata gcagtatctt ggactgcatt gtcagggaaa
    11461 atcgcgcgac atcatctgcc cgcgtttacg gaagagttgc ggcgcatcat ctgcggcggt
    11521 aaaaatcccg atgcgggctg taaacaagcc ggacaggttg ggaaaaggca tcagaaataa
    11581 atgatagccg tctgaaatat tgaagagggc atccgattga ttgaaccatc aaacccgaaa
    11641 gcaaccctat ggaaaaaaaa ttcttagaca tcctcgtctg ccccgttacc aaaggcaggc
    11701 tggaatatca tcaggacaaa caggaattgt ggagccgtca ggcgaagctt gcctatccga
    11761 ttaaagacgg cattccctat atgctggaaa acgaagcgcg tccgttgggc gaagaggaac
    11821 tcgaagcatg accgaattcg tcgtattgat tccggcgcgg ctggattcgt cgcgcctgcc
    11881 cggaaaagcc ttggcggaca tccacggcaa accgatggtc gtgcgcgttg ccgaacaggc
    11941 ggcaaaaagt aaagctgtgc gcgtcgtcgt tgccaccgac catcccgata ttcagacggc
    12001 ctgtcaggcg cacggcgtcg aagtcgtcat gacttcaaac cggcacgaaa gcggcacgac
    12061 gcgccttgcc gaagcctctg ccgcgctgaa gctgccgccg catctggtgg ttgtcaacgt
    12121 acagggcgac gagccgctga ttgcacctga actcatcgac cgcaccgccg aagtactcgt
    12181 cgaaaacaac gtccaaatgg cgaccgccgc ccacgaattg cacgatttcg acgaattgat
    12241 gaatcccaat gccgtcaaag ttgtcctcga caaaaaccgc aacgccatct acttcagccg
    12301 cgccccgatt ccctatccgc gcgatgcgat gcgtgcagga aaacgcgaaa tgccgtttga
    12361 aaccgccgtc ctgcgccata tcggcatcta cgcctaccgc gtcggcttcc tgcaacgcta
    12421 caccgaaatg agcgtttcgc cgctggaaac cattgaatcg ctggagcaac tgcgtgtcct
    12481 gtggcacggc tacccgattg ccgtcgaaac cgccaaagaa gctcctgccg ccggcgtaga
    12541 tacgcaggag gatttggaca ggattcgcgc tgtatttcag actatataaa atagattcaa
    12601 agggaaaaga tatgcagcaa catattgaaa aatggcaaca cttgagccgg gaagaacaga
    12661 aaatccttgc tgaagtatgg ggtctcgtgc aaaacgacga tcaggaggtt cactatgaaa
    12721 tgctcaaatt gaacgcaccc gacgaagcca gtggcgaatt ttggttcaga atggcagaaa
    12781 cactcagcac cctgccgccc aaccgttccc tcgaccttag aatgaacggc ggcaggctgg
    12841 cgaccgccgt atccatcctt tccgtcatga ttgaagacaa tcccgacata ccgcagcttt
    12901 gggcgcaaaa aattaccgcg ctcaattata gtggattaaa tttaaaccag tacggcgttg
    12961 cctcgccttg tcgtactatc tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt
    13021 taatccacta tatttggcac acggacacaa agcccgtgcc gacggtttgg cacaacagcc
    13081 cgacaaagcg gcagaagcca acgaggagga atacctgacc aaagccctgt cgcaaaacct
    13141 actgtcaaca ttggatgccg cgcttgcacg ttttcctgaa gacgcgtggt ttcaggaaat
    13201 caaacaggat gcacaaaagc attttgcttg aggatgtggc agtcaggaat atttccattc
    13261 aggaagaaaa gaagtgcctg attgggtata atcagggtaa atcttatttt atttcaaaag
    13321 attaatattt gctttctgtt tttccttgac ggtatcggaa aagttgatta tagttacagc
    13381 ttccttagga gtaatggctg agaggctgaa ggcacttccc tgctaaggaa gcatgtgggg
    13441 tcaacctgca tcgagggttc gaatccctct tactccgcca agatgagaag acgctgtgtt
    13501 ttacagcgtc tttttttacg cgctttgttg cggaagtgcc gagggctgtc tgccgtgcgc
    13561 cgcctggatt tgcaatgtgc ccgcccgtgc aaaatccgat ttgaccaacc tgaagggcag
    13621 gtttcggaag agcggggaat tttgataaac aaaaaactgc acggcataat ctttcccggt
    13681 tttcattttc cgtattcgcc tatccgtacc gcaaatgcta tactgggaaa aatttaccga
    13741 ttgtgtttta cggcatattt gccgatagga tggaagagac aaatgagcag aatccggcag
    13801 gcttttgccg ctttaaacgg tgaaaaggca ttgattcctt atattacggt tggagacccg
    13861 gacattgggg cgactttggc aataatgcac agtatggtgg aaaacggtgc ggatattttg
    13921 gagttgggcg tgccgttttc cgatccgatg gcggatggac cggttattca gcgtgcggcg
    13981 gagcgggcgt tggcaaacgg gatttcgctg cgcgatgtct tggatgtcgt cagaaaattc
    14041 cgtgaaaccg acacgcaaac gccggttgtt ttgatgggat atttgaaccc tgtacataag
    14101 atgggttatc gggagtttgc tcaggaagcc gcaatggcgg gtgtggacgg cgtgttgacg
    14161 gtggattccc ctgtcgaaac catcgatccg ctctatcgcg agctgaagga taacggggtc
    14221 gactgtattt tcctgattgc gccgacgacg acggaagacc gtattaaaac cattgccgag
    14281 ctggcaggcg gatttgtcta ttatgtttcg ctcaagggcg taacgggcgc ggcaagtttg
    14341 gatacggatg aggtttcgcg taaaatagag tatttgcatc agtatatcga tattcccatc
    14401 ggtgtcggtt tcggcatcag caatgcggaa agcgcgcgca aaatcggccg ggttgccgac
    14461 gcggtcattg tcggcagccg gattgtgaaa gagattgaaa acaatgcggg caacgaggct
    14521 gcggtcgtcg gtgctttggt caaagagttg aaggatgccg tgcgctgatt ctgtcacgca
    14581 ttctaaatat tttaggagtt gtccatgagc tggttagata aaatcctgcc acccaaaatc
    14641 aaaaaccgca gcaaagacgg ttcttccaat gttcccgagg gtttgtggca caaatgtccg
    14701 tcttgttcgg caaccgttta ttcgaccgag ttgcagcaga acaatcaggt ttgtccgaaa
    14761 tgcaaccacc acaatccgtt gtcggcacga caacgcctga acctgctttt ggatgaggat
    14821 ggcagggagg aagttgccgg aaatgtcaaa ccgatagatc ctttgaagtt taaagacagt
    14881 aaaaaatatc ccgaccgttt gggggcggca cgcaagctga ccggggaaga tgacgcgctg
    14941 gtggtgatga aggggatgat gaacggcctg cccgtcgtcg ttgctgcgtt tgaattccgc
    15001 tttatcggcg gttcgatggg ttcggttgtg ggagagcgtt tcgtacaggg cgtccgccgt
    15061 gcggttgccg acaattgtcc gttcgtctgt gtggcggctt ccggcggtgc gcgtatgcag
    15121 gagggtgtga actcgttgat gcagatgacg aaaaccagtg ccgcgctgca tttgctgacg
    15181 gaaaaacgcc tgccgtttat atcggtattg accgatccga ctatgggcgg cgtatccgcc
    15241 agcttcgcat ttttgggtga tgttgtgctt gccgaaccga acgcgctgat cggttttgcc
    15301 ggtccgcgcg tgattgagca gacggtgcgc gaaacgctgc cggaaggctt ccaacgcgcc
    15361 gagttcctgc tggaaaaagg cgcaatcgac cagattgtcg accgccgcga tatgaagcgg
    15421 cgcatcagtg atttgattac gctgttgtgc cgtcaggaca aagtttccgc cgcttgatgg
    15481 ctgatgaatc gaataccgtc tgaaaccgat gtttcagacg gtatttttgt gtctggttat
    15541 ttgttgtgcg gcttcatcga cggggcatag cgtccggcac gttctttcag gcgttgtacc
    15601 aaacctttcg tgtcggcggg tacaccgccc tcgcagaatg cctgatacag gacggtgcgc
    15661 agtgcgtcgt tgcggcttaa tgtaccgcct atcggtttcc attcggcgtt tcggggctgt
    15721 atccagcggc ggttgaccgt gtcgccgtag ccgaacactt tataggagga aggtttgccg
    15781 ttgccgaacc tgatggagaa gcgggcgcag aatatgcctt cggcagtcag gttgtcgtag
    15841 cctttgtcgg agcggatatt gagaatgtag cggatgctgc cgtcgggcgc gggcataatt
    15901 tgcaggctgt cgagcaggat tttcggctgt ttgccgtaat tttcatccac ataaatgtcg
    15961 aaccagccgt ccgagtgcgt atcgggcagc ggcggcagtt tggcggtatg ttctttaaat
    16021 tcgcgggcgg cggcctcttc gggcgtttcg cggtagcggg tgttgatcgg cgtgtctttt
    16081 tggctgaagc cggcagcgag ggacgtgccg gcggcgagtg cgagcagcag gagggcggtg
    16141 cggcgcataa gtttctccaa attgaaaacg gcgttatttt atgggttggc aaagggggct
    16201 gcaagcaact ggggtataat ctccccccga ttcccatttt ttaaacggta caaacgatga
    16261 acagcgaaac tttagacgta accggattga aatgtcccct gccgattttg cgggcgaaaa
    16321 aggctttggc gcaaatgcag cagggcgacg tgttgaccgt tctggcaacc gacggcggcg
    16381 caccggggga ttttgaggct ttttgccgcc aaaccggtca tgtgctgttg gatgcttccg
    16441 aacaggacgg cgtgttcacg ctggtcgtcc aacacaaata aatgccgtct gaaatgcgga
    16501 tgtcccgatt gatgttattg ctgtcggcat ttgtgcttgc tgcgtgtaat gcccaaaacc
    16561 ggcaatccgg tgtacggcat caggatttcg cgcgtgtagc cgtcgatttg tttcggagtg
    16621 cctgcgtcct gactcaaggc agttttgaat cggtttccaa gtttgccgct gtcggacatt
    16681 ttgtccctgt cggaaaggaa gacctgtccc atctgccgcc cactgttgca gaacccgatg
    16741 tacaggcgtt gtggacgctt gaacgcgatg gcggcattta ttatttgagc ctgacgcgcg
    16801 acagttgcag tgtgaaagcc gagcgggcag acagtgccgc gctgttggaa cattttgacg
    16861 gacttgtccg acagccgccg aaaaatgcca atatagaatt gagggcggaa cagtcggtgc
    16921 aggcatcatt tgaaatccgc caagtcagct atgcctggcg ggaagcgggc agctccgaag
    16981 aaaccgtatt gaccgcacaa accgccgatt ccccggattt acccgtacag gcagttttga
    17041 acctgacgca ccgttcccac aacgggaaac ccctaattct cccctaactt cagacggcat
    17101 aaagtcggca tgccgtctga acattcaaat aaaacaaagg aaaaccatgc aaaccctgac
    17161 cattatccgc cccgacgata tgcacctgca cctgcgcgac ggcgacgcgc tcaaagccgt
    17221 tgctccttat accgcccgcc aaatggggcg tgccgtcatt atgcccaacc tcaaaccgcc
    17281 tgtcgtcagc gttgccgacg cgcttgccta caaagcgcgc atcatggcgg cattgcccga
    17341 aggcagcgcg tttgagccgt tgatgacgct ttacctgacc gaccaagcca cgcctgcact
    17401 tgtgcgcgaa gccaaagccg ccggcatcgt cgccttcaaa ctctaccctg ccggcgcgac
    17461 caccaattcc gattccggcg taaccgacct gttcaagctc atccccgtgt tggaagaaat
    17521 ggcgaaacag ggcattttgt tcctcgttca cggcgaagta accgaccccg aaatcgacat
    17581 cttcgaccgc gaagccgcct ttatcgggcg cgtgatgaaa cccgttttgg cgcaagtgcc
    17641 gaatcttaaa gtcgtgttcg aacacatcac caccgccgaa gccgcccgcc tggttttgga
    17701 agcaggcgac aacgtagccg ccaccgttac cccgcaacac ctcctgctca accgcaacga
    17761 cctcttggtc ggcggcgtgc gcccccatca tttctgcctg cccgtactca aacgcgaaac
    17821 ccaccgtcag gcattggttg ccgccgttac cggcgagaag gcgcacaaat tcttcctcgg
    17881 caccgactcc gcgccgcacg ccaagtccgc caaagaaaac gcctgcggct gcgccggtat
    17941 gttcagcgcg atgaccgccg tcgagcttta cgccgaagtg tttgaaaaag caggcgcgtt
    18001 ggacaaactc gaagcctttg cctcgaaaaa tggcgcaagg ttttacggca ttccggaaaa
    18061 cgccggcacg atcaccctcg tcaaacaaag ccaaaccgtc cccgcaagtg ttccctacgg
    18121 cgacggcgaa cttgtcccga tgcgcgcggg cggcgaaatc ggctggacgg tgcagtattg
    18181 atgggctgga aacaaaatgc cgtctgaagg gagggctttc agacggcatt tgtgttgctg
    18241 actgattgat acgtcaacgg cgtttgagtt tgttacgttt cggttatttc cgataaattc
    18301 ccacaatttt caaatttcgc cattcccacg aaggcaggaa tccagaaatt cgatgcgacc
    18361 aaagtttatc aaaaacggca gcaactcaaa aaaccggatt cccgcctgcg cgggaatgac
    18421 gagattgaag tttcagaatt tatttgaaat acccaaaatt caaaaaacca aattcccacc
    18481 tgcgtgggaa tgacgaaaca aagaaagcag aaataaggac atagaacttt ctttaaattt
    18541 gtgatgcatc aacggcgttt gggctcgtcg gggcggattt gggcggcgag tttgtcgagg
    18601 atgccgttga cgaatttgtg cccgtccgtg ccgccgaagg ttttggtaac ttcgatggct
    18661 tcgttgatga tgacggggta gggtgtttcg ggcatggcgg acagctcgtg gcaggcggtc
    18721 agcaggacgg cgcgttcgat gggattgagg tctttttcgt cgcggtcgag cagggggcgg
    18781 attttttgga tgtagtccgc tgcgttggtt tgcgtaccga agaaaagttt gttgaacaat
    18841 tcttcgtccg ctttggcaaa gtcgggcatt tcgcggatgt ttttagcaat ttcgggcgcg
    18901 gcggtgcggt tgataaggga ttggtaaacg gcttgtacgg caagctcgcg ggaacggcgg
    18961 cgggctgttt tcatgatttt tccttgaaac ggttgggcgg cacggtatgc cgtctgaaac
    19021 ggaaagggcg tattggtgta cgccctgttt gttattcttc gtcttcaaac tgttcttcga
    19081 gcagcaggtt gacgaggttg gcgcattcga cggcgacttt ggcggcatcc gaggcttttt
    19141 cttcaatccg ttcgattgcc tgcgcgtcgt tttcggtggt taggacggca ttggcaatcg
    19201 ggatgttgta gtcgagtgcg acgcggctaa cccctgctcc ggattcgttg gaaaccagct
    19261 cgaaatggta ggtttcgcca cggatgacga caccgatggc aatcagtgcg tcaaactttt
    19321 cggaagaggc aaggttcatc agtgcgatgg ggatttcaag tgcgccgggt acggtggcga
    19381 cggtaatgtt ttcgtctgcc acgcccaatt cttggagggt gcggcagcag actttgagca
    19441 tttcgctgcc gatttcgttg gtgaaacgtg cctgtacgat gccgatgcgg aggtgtttgc
    19501 cgtcgaggtt gggggcgatg gtgttcattg ggtgtccttt ggtattcgga ggtttcggaa
    19561 tgccgtctga aggtttcagt cttgcggctg ccagtcggcg acggtttgga atgtgccgtc
    19621 ttcggcaagc tcccatgcgc tgccttcggg ttgggaaagc agtgcggcgg tttcagggtt
    19681 ggttttggcg atgtcggcga ggctgacgat gctgaagttg tcgggatcgt cggtgtattc
    19741 gtcggtctcg tcgccgctga agaaacgcca gccgctgtcg ttttcaaaaa cgggggcttc
    19801 gcggtagagg aagccgacgg gccggttttg tttggcgacg gtgttggtgg cgatacagcg
    19861 gtcgagtgcc gaggaaagtg cttgtgcaaa tgcgttcatt acgggaatac gttgggggaa
    19921 aacttacgga ttttaccacg attcgtgcgt tgtcggcaga cggcggcggt ttggtggtac
    19981 aatgtgcgcc gtttgcagcc ttaaggtgtt tctgtatttt tggagtatgg aaacgcattc
    20041 gggctgtttt ttgcggaaga cggtaatgaa agacgatgtt ttgaaacagc aggcacacgc
    20101 ggcgatacag aagagactgg gctacgcgtt ccgcgatatt tcgcttttgc ggcaggcttt
    20161 gacgcacagg agccatcatg cgaagcacaa cgagcggttc gagtttgtcg gtgattcgat
    20221 tttgaattat acggtggcgc ggatgctgtt tgacacgttt ccgaagttga ccgagggcga
    20281 gttgtcgcgg ttgcgggcaa gtctggtcaa tgagggcgtg ctggcggaaa tggcggcgga
    20341 aatgaatgtc ggcgacggtc tgtacttggg ggcgggcgag ttgaagagcg gcggcttcag
    20401 acggccttcg atactggcgg acgcgatgga ggcgatgttt gcggcggtca gcttcgatgc
    20461 cgatttcaac acggcggaaa aggtggtgcg ccatttgttt gccgaccgcg tccggtgtgc
    20521 cgatttccaa aatcaggcaa aagacggcaa aactgctttg caggaggcgt tgcaggcacg
    20581 ccgctttgct ttgccgaaat accgtatcga agagcaaatc ggccatgcca acgacagtat
    20641 gtttgtggtt tcctgcgatt tgggcgaact gggtttcgtg tgccgtgcca aagggacgag
    20701 ccgcaaggcg gcggaacagg aggcggcgaa agaggctttg aaatggctgg aagagaagct
    20761 gccgctgaag aagaaaaaga aatgaggcgg cgcgtgaata tgccgtctga aatgtggata
    20821 tgaaagcgaa tatggatatt gaaaccttcc ttgcagggga acgcgccgcc ggcggatacc
    20881 gttgcggctt cgtggcgatt gtcggtcgtc cgaacgtggg caaatcaacg ctgatgaacc
    20941 atctcatcgg tcagaaaatc agcatcacca gcaaaaaggc gcagacgacg cgcaaccgcg
    21001 taacggggat ttataccgac gataccgcgc agttcgtgtt tgtcgatacg cccggctttc
    21061 aaaccgacca ccgcaacgcg ctcaacgaca ggctgaatca aaacgttacc gaggcgcttg
    21121 gcggcgtgga tgtggtggtt ttcgtcgtgg aagcgatgcg ctttaccgat gccgaccgtg
    21181 tcgtgttgaa acaactgccc aagcacacgc cggtcatttt agtggtcaac aaaatcgaca
    21241 aggacaaggc gaaagaccgt tacgcgctgg aggcgtttgt tgcccaagtg cgcgccgaat
    21301 ttgaatttgc ggcggcggag gcggtcagtg cgaaacacgg attgcggatt gccaacctgt
    21361 tggagctgct caagccgtat ctgcccgaaa gcgtgccgat gtatcccgaa gatatggtta
    21421 cggacaaatc ggcgcgtttt ttagcgatgg aaatcgtgcg tgaaaaattg ttccgctatt
    21481 tgggcgagga attgccttat gcgatgaacg tcgaagtgga gcagtttgaa gaggaagacg
    21541 gtttgaaccg catctatatc gccgttttgg tcgataagga aagccaaaag gcgattttaa
    21601 tcggcaaagg cggggagcgt ttgaagaaaa tttccaccga agcgcggttg gatatggaaa
    21661 aactgtttga taccaaagta tttttgaagg tctgggtcaa agtcaaatcc ggttgggcgg
    21721 acgacatccg cttcctgcgc gagctgggtt tgtagttttt cttgctgaac tttacgcaaa
    21781 tgccgtccga acaggtttca gacggcattt tgtttcaatc gggaatatct ttgttaaaaa
    21841 cgggttgata ttatctgt
//
LOCUS       DAXHNE010000017        21294 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.017, whole genome shotgun sequence.
ACCESSION   DAXHNE010000017 DAXHNE010000000
VERSION     DAXHNE010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21294
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.017"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(59..1945)
                     /gene="uvrC"
                     /locus_tag="ACFM4Z_000506"
     CDS             complement(59..1945)
                     /gene="uvrC"
                     /locus_tag="ACFM4Z_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFC6020594.1"
                     /translation="MPSETVFQVIDMNKETRFPEHFDIPLFLKNLPNLPGVYRFFNES
                     GNVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSESEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVREAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGLVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPVPDALKEALEGEHGKQMQFVTKTIGERKVRLKMAEQNAQMAIAQRRLQQSSQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            complement(2009..4171)
                     /locus_tag="ACFM4Z_000507"
     CDS             complement(2009..4171)
                     /locus_tag="ACFM4Z_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC6020595.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLFTINVPFLIG
                     MAGMMIGRHDWMIPPLWQFALASVVQLWLAIPFYKSAWASIKGGLANMDVLVTIGTVS
                     IYLYSVYMLFFSPHAAYGMAHVYFEVGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKL
                     TPTQVNVQRNGEWKQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHLTGESN
                     PEEKKAGGKVLAGALMTEGSVVYRATQLGSQTQLGDMMNALSEAQGSKAPIARVADKA
                     AAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAIMVGMG
                     KAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGSPQVAAVYCVPDSGFDEDALYRI
                     AAAVEQNAAHPLARAIVSAAQARGLDIPAAQNAQTVVGAGITAEVEGVGLVKAGKAEF
                     AELALPKFLDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNIDVYIM
                     SGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDAPALAA
                     ANVSFAMKGGADVAEHTASATLMQHSVNQLADALLVSQATLKNIKQNLFFAFFYNILG
                     IPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            4310..5260
                     /gene="trxB"
                     /locus_tag="ACFM4Z_000508"
     CDS             4310..5260
                     /gene="trxB"
                     /locus_tag="ACFM4Z_000508"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFC6020596.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            5416..5784
                     /gene="rpsF"
                     /locus_tag="ACFM4Z_000509"
     CDS             5416..5784
                     /gene="rpsF"
                     /locus_tag="ACFM4Z_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFC6020597.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            5785..6087
                     /gene="priB"
                     /locus_tag="ACFM4Z_000510"
     CDS             5785..6087
                     /gene="priB"
                     /locus_tag="ACFM4Z_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFC6020598.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            6094..6324
                     /gene="rpsR"
                     /locus_tag="ACFM4Z_000511"
     CDS             6094..6324
                     /gene="rpsR"
                     /locus_tag="ACFM4Z_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFC6020599.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            6341..6793
                     /gene="rplI"
                     /locus_tag="ACFM4Z_000512"
     CDS             6341..6793
                     /gene="rplI"
                     /locus_tag="ACFM4Z_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFC6020600.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            complement(6986..7792)
                     /locus_tag="ACFM4Z_000513"
     CDS             complement(6986..7792)
                     /locus_tag="ACFM4Z_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6020601.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAIMVFTAFSSMLGQHKKRAVDWKTVFAMMPGMIFGVFAGALS
                     AKYIPAFGLQIFFILFLTVVAFKTLHTGPQTASRPLPKLPGLTAVSTLFGAMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(7793..8539)
                     /gene="pssA"
                     /locus_tag="ACFM4Z_000514"
     CDS             complement(7793..8539)
                     /gene="pssA"
                     /locus_tag="ACFM4Z_000514"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFC6020602.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            complement(8613..8738)
                     /locus_tag="ACFM4Z_000515"
     CDS             complement(8613..8738)
                     /locus_tag="ACFM4Z_000515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020603.1"
                     /translation="MPGNNIHPAKIRNNTPGGRQSQTAPQSIRHQKNKPPPRASS"
     gene            9122..9604
                     /locus_tag="ACFM4Z_000516"
     CDS             9122..9604
                     /locus_tag="ACFM4Z_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HFC6020604.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGVMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGVQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(9656..10867)
                     /locus_tag="ACFM4Z_000517"
     CDS             complement(9656..10867)
                     /locus_tag="ACFM4Z_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HFC6020605.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQINWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            11090..13528
                     /locus_tag="ACFM4Z_000518"
     CDS             11090..13528
                     /locus_tag="ACFM4Z_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC6020606.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDEPEPLEKLPLIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSSTD
                     LNGSRKILAHKDHL"
     gene            13736..15046
                     /gene="tig"
                     /locus_tag="ACFM4Z_000519"
     CDS             13736..15046
                     /gene="tig"
                     /locus_tag="ACFM4Z_000519"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFC6020607.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVQKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YADPSRLQAPTSLAVESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            15142..15756
                     /gene="clpP"
                     /locus_tag="ACFM4Z_000520"
     CDS             15142..15756
                     /gene="clpP"
                     /locus_tag="ACFM4Z_000520"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFC6020608.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCDRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLRL"
     gene            15780..15974
                     /locus_tag="ACFM4Z_000521"
     CDS             15780..15974
                     /locus_tag="ACFM4Z_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020609.1"
                     /translation="MPSETFRRHLFQAAFDMVSIPSSTGRKKDLEFFKGSPFPPVMDG
                     CRLKAKWGSSFCKGATLASS"
     gene            complement(16199..17464)
                     /gene="ispG"
                     /locus_tag="ACFM4Z_000522"
                     /gene_synonym="gcpE"
     CDS             complement(16199..17464)
                     /gene="ispG"
                     /locus_tag="ACFM4Z_000522"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFC6020610.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSSLESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGLKRHQGKVIPIHSL"
     gene            complement(17480..18241)
                     /gene="pilW"
                     /locus_tag="ACFM4Z_000523"
     CDS             complement(17480..18241)
                     /gene="pilW"
                     /locus_tag="ACFM4Z_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HFC6020611.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            complement(18244..19338)
                     /gene="rlmN"
                     /locus_tag="ACFM4Z_000524"
     CDS             complement(18244..19338)
                     /gene="rlmN"
                     /locus_tag="ACFM4Z_000524"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFC6020612.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVKDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(19481..19906)
                     /gene="ndk"
                     /locus_tag="ACFM4Z_000525"
     CDS             complement(19481..19906)
                     /gene="ndk"
                     /locus_tag="ACFM4Z_000525"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFC6020613.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            complement(19965..21116)
                     /gene="zapE"
                     /locus_tag="ACFM4Z_000526"
     CDS             complement(19965..21116)
                     /gene="zapE"
                     /locus_tag="ACFM4Z_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFC6020614.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLTEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
BASE COUNT         5109 a         5293 c         5778 g         5114 t
ORIGIN      
        1 ggtataccga cctgctgtat catatttttt acagacggat tttgtctttg acagcatgct
       61 aatgcagatg attgtaaatc gtttccgcca atgccttgct gatgccttcc actttttcca
      121 agtcctcgcg gctggcggca atcacgccgc gcagaccgcc gaagcgggtg agcagggctt
      181 ggcggcgttt gctgcctacg ccggggatgt cgcttaagga ggaggtaacg cgggctttgt
      241 cgcgtttttt gcggtgaccg gtaatggcga aacggtgcga ttcatcgcgt acggtttgca
      301 atagatgcaa ggccgggctg ttgggcggca ggcggaagac ttcgccggta aaaggcagta
      361 tgagctcctc cataccggct ttgcgctccg ggcctttggc aatgccgacc aaagggatgt
      421 gcagcccgag ttcttcccat accgatacgg ctacgccgat ttgccctttg ccgccgtcaa
      481 tcaacacggc atccggccat ttgacggtct cgccgttggc ttcggcctcc tgcattttgc
      541 cgtaacggcg cgtcaacact tcgcgcatgg cggcgtagtc gtcgccgggt ttggcggtcg
      601 tgatgttgta gcggcggtat tgcgaaggct ggatgttttg ctcatcgtac acaacgcagg
      661 acgcaatagt ggcttcgcct tgtgtgtggc tgatgtcgaa acattcaagg cggttgaggc
      721 cgtctgaatc catgccgagg atttttgcca gttcatcaat gcggtgttgc tggctgcttt
      781 gttgcaggcg gcgttgtgca atcgccattt gcgcgttttg ttccgccatt ttcaaccgga
      841 ctttgcgttc gcctatggtc ttggtgacaa attgcatctg cttgccgtgt tcgccttcca
      901 aagcctcttt tagcgcatcg ggaacgggaa agttgctgat gatgatgtcg ggtttgcttt
      961 tgcccagata gtgttgggcg acgaaggctt cggcgtaatc ttgtccgttt ggctcgggat
     1021 cgtttttggt gtcggggaaa aagcttttgt cgccgacgtg ccgtccgccg cggatgctga
     1081 cccagtgtac gcaaaccagg ccgtctgaaa ccgccagtgc aagcaaatcg atatcgttgg
     1141 gattgttcgg atttttactg tcgataaact gattactttg catgatgccg agcgcttgga
     1201 tttgatcgcg gtaacgtgcg gcttcttcga attgtagatt agcggcggcg gtttgcattt
     1261 tgtgttgcag ggtacgcgtc aattcgtcag ttttgccatt aaggaaagtc gcggcttcac
     1321 gcacgctgtc acgataatct tcttcactga tgtggcctac acaaggcgcg gtgcagcgtt
     1381 tgatttggta aagcagacaa ggacggtcgc gatgctcgaa tacactgtct tcgcaggtac
     1441 gcagcataaa gactttttgc aacacttgaa tgctgtcgcg cacggcgttg ctgttgggat
     1501 atgggccgaa atattgatta ggctttttca gcgtgccgcg gtaatacgcc atttgcggat
     1561 attgatggcc gctgagcatc aaataaggat agcttttgtc atcgcggaaa agaatattgt
     1621 atttgggcga cagggctttg atgaagttgt tttcgagaat cagggcttcg gattcggagc
     1681 gggtgatggt ggtttcgatg tggtgaacct gtttcaccat caatgcgatg cgcggggaat
     1741 ggtcgttttt ctggaaatag ccggacacgc gccgcttgag gttgacggct ttgccgacgt
     1801 ataagacgtt gccgctttcg ttgaaaaaac ggtatacgcc tggcaggttg ggcaggtttt
     1861 tgaggaaaag tgggatgtcg aagtgttccg gaaaacgggt ttctttattc atatcaatta
     1921 cttgaaaaac ggtttcagac ggcatattac catatcgctg gagacagcct tatccgttcg
     1981 gacaaggctg cagggcggtt acatgctgct aatcgatttt tacccgtttc aggcgcaagg
     2041 cattgctcaa cacggaaacc gagcttgccg ccattgccgc gccagcgatg acgggattta
     2101 aaaagccaag cgcggcgaga ggaatgccca aaatattgta gaagaaggcg aaaaacaggt
     2161 tttgcttgat gtttttcaaa gtggcttgcg acaccagcag agcatcggcg agttggttga
     2221 ccgaatgctg catcagcgtg gcggatgcgg tatgttcggc aacgtccgct ccgcctttca
     2281 tggcgaagct gacgttagcg gcggcaagcg cgggcgcgtc gttgatgccg tcgccgacca
     2341 tcgccacggt tttgccggcg gctttgagtt tttgcacttc ggcagcttta tcgcgcggac
     2401 tcatgttgcc gaaggcgtgt gcgatgccca gttgtttggc gacgtattcg accgtgcctt
     2461 ggttgtcgcc gctcataata tagacatcga tattgtgttt tttcagacgg cctatggctt
     2521 cggcggtatc ggctttcaac gcgtcggcaa gtgcgaatgc gccgatgggt ttgttatcga
     2581 ctgagaccgc aacaatgctt gcaatatccc aaacgccgtc taaaaacttc ggcaaggcca
     2641 gttcggcaaa ttcggctttg cctgctttca ccaaacccac gccttccact tcggcggtaa
     2701 tgcctgcgcc gacaacggtt tgtgcgtttt gtgcggcggg aatgtccaaa ccgcgcgctt
     2761 gggcggcgga gacgatggca cgggcgagcg gatgggcggc gttttgttcg acggcggcgg
     2821 cgatgcggta caaagcgtct tcgtcaaagc cgctgtcggg aacgcaataa acggcggcaa
     2881 cctgcgggct gccttcggtc agcgtaccgg ttttgtccaa cacgacggca tcgacgtggg
     2941 cggcttcctc cattgctgcc gcgtctttaa accaaatacc gtgtttaacc gctttgccca
     3001 taccgaccat aatcgcggca ggggttgcca gacccagcgc gcacgggcag gcaatcacca
     3061 aaacggcgac ggcgtgcatc agcgcaaccg tccaatcgcc cttaatcagc caagtaacaa
     3121 taaaagtcaa caacgcaatg cccacgacgg caggcacgaa taccgcagcc gctttatcgg
     3181 ctacgcgcgc aatcggtgct ttactgcctt gtgcttcaga gagcgcgttc atcatgtcgc
     3241 cgagctgggt ttggctgccg agctgcgtgg cgcggtacac cacactgcct tcggtcatta
     3301 acgcgcccgc caacactttg ccgcccgcct ttttttcttc aggattggat tcgccggtaa
     3361 gatggctctc gtccgcccaa ccgctgccgc tttcaatgat gccgtctgcg gcaatgcgtt
     3421 cgccgtggtt ggcgcggata aggtcgccga tttgcacttg gtcgatggga agctgtttcc
     3481 attcgccgtt gcgttgcacg ttgacttggg ttggtgtaag tttgagcagc aagcccaagc
     3541 tgttgaggct ggattttttg gtacggtgtt ccaaaaattt acccagtgac acaaaaccga
     3601 tcaccatcac gcccacttca aaatacacat gcgccatacc gtacgccgcg tgcgggctga
     3661 aaaacagcat atagacggaa tacaggtaaa tcgagaccgt gccgatggta accagcacgt
     3721 ccatattcgc cagtccgccc ttaatgctcg cccacgcgct tttgtaaaac gggattgcca
     3781 gccaaagctg caccacgctt gccaatgcga actgccacaa cggcggaatc atccaatcgt
     3841 gtctgccgat catcatcccc gccatgccga taaggaacgg gacgttgatg gtgaacagca
     3901 gccacagccg ccagccgata tggtgttctg cttcgggttg cggcaatgta tcttccgttt
     3961 tttccttcgc gccgtaaccg gttttctcaa tgattttggc aatgtcggct actgaggttt
     4021 tgctgtcgtc aaacactacc tgcgcctctt cgctggcgaa gtttaccccc gccgattcga
     4081 caaaatcttt tttgttcaac actttttcaa tgcgcgaagc gcaggcctgg caggtcatgc
     4141 cttcgatttg gaaacggatt ttttgttgca taagcgtgat tcctgctttg atattcagac
     4201 ggcagttaag gctgcgggat gccgtctgag cgccggtatg gtcgaaattt ctcaaacgcg
     4261 ccgataattc gagtagaatc cgaaaacttg acacagcaaa ggaaaaacaa tgtcccaaca
     4321 ccgcaaactg attattttgg gttccggccc cgccggatac accgccgccg tctatgccgc
     4381 gcgcgccaat ttaaaccccg tcattattac aggtatcgcg caaggcgggc aactgatgac
     4441 caccaccgaa gtggacaact ggcctgccga tgccgacggc gtgcaagggc cggaattgat
     4501 ggcgcggttt ctcgcccacg ccgaacgttt cggaacggaa atcatttttg accaaatcaa
     4561 cgccgtcgat ctgcaaaaac gcccgtttac actcaaaggc gatatgggcg agtacacttg
     4621 cgatgccctg attgtcgcaa caggcgcgtc cgccaaatac ctcggtttgc cgagtgagga
     4681 agcgtttgca ggaaaaggcg tttccgcttg tgccacctgc gacggtttct tctataaaaa
     4741 ccaagatgtt gccgtagttg gcggcggcaa tacggcagtt gaagaggcac tctaccttgc
     4801 caatatcgcc aaaaccgtta ccctgatcca ccgccgcagc gagttccgtg ccgaaaaaat
     4861 catgatcgac aaactgatga aacgcgtgga agagggcaaa atcatcctca agctggaaag
     4921 caacctgcaa gaagtactgg gcgacgatcg gggcgtaaac ggcgcattat taaaaaacaa
     4981 cgacggttct gagcaacaaa ttgccgtcag cggcattttt atcgccatcg ggcacaagcc
     5041 gaataccgat attttcaaag gtcagttgga aatggacgaa gccggttacc tgaaaaccaa
     5101 aggcggcacg gcggacaatg tcggcgcaac caatatcgaa ggcgtatggg cggcgggcga
     5161 cgtaaaagac catacctacc gtcaggcgat taccagcgcg gcttccggct gtcaggcggc
     5221 tttggacgcg gaacgctggc tgggcagcca aaacatttga atgccgtctg aaaccgaaat
     5281 tttcggcgga atgcaaattt ttcgcaccaa atccttgatt ccgtcggcaa accccaatat
     5341 aatgccgctt ctttaaacct tgccttctgc tttcgcatgg cagaaggctg tttttaccat
     5401 ccataaggag ataacatgcg tcattacgag atcgtgttta tcgttcatcc cgatcaaagc
     5461 gagcaagtac ccgctatggt tgaacgttac aaaaccatga ttgccgaagc aaacggtaag
     5521 attcaccgtc tggaagactg gggtcgccgc cagctggctt acccgattaa caaaatccat
     5581 aaagcccatt acgttttgat gaacatcgaa accactcccg aagtggttga agagctggaa
     5641 accgcattcc gcttcaatga tgcagtattg cgtcatctga ccatcaaaac caaacacgcc
     5701 gttaccgaag catcccctat gttgggtggt gaaaaggcta agaacctgtt gagcggtgcg
     5761 tctgaagaag cggtcgccca ataattggga ttcaataatc ttgtttcgct tgccgcgtta
     5821 attgaaaagg ttttccctat tcgatatacg cctgccggaa tccctgtttt agatattatt
     5881 ttaaagcacg aatcgtggca ggaggaaaac gggcagcaat gccttgtcca attggaaatt
     5941 ccggcacgga ttttaggcag gcaggcggaa gagtggcagt atcggcaagg tgtatatgtt
     6001 cacgtcgaag gttttttagc tcaaaaaagc agacgttccc ttatgccgat gctcaggata
     6061 caaaatattc aagaatataa aggttaaacg acaatggctc gtcaatcatt caaacgtaga
     6121 aaattctgcc gtttcacggc tgaaaaaatc caagaagtcg attacaaaca agttgatttg
     6181 ctgaaagact tcatctccga aaacggtaaa atcattcctg cacgcatcac aggaacgaag
     6241 gcattctacc aacgccaatt ggctgttgcc gtaaaacgcg cacgcttcct ggctctgctg
     6301 ccttacaccg accaacacaa ataattttgg agattgaatc atgcaaatta ttctgttaga
     6361 aaaaatcggc ggtctgggca acttgggcga catcgtaacc gttaaaaacg gctacgcccg
     6421 caactttcta attcccgcag gtaaggcaaa acgtgcgacc gaagcgaata tgaaagagtt
     6481 tgaagcacgc cgcgcagaac tggaagccaa acaggctgaa attttggcag atgcccgagt
     6541 ccgtcaggaa aaattggacg gtcaaaccgt taccgttgct caaaaagctg gtgtggacgg
     6601 tcgcctgttc ggttccgtta ccaatgccga cattgctgct gcaatcgttg ctgccggcat
     6661 cgaagccgtg aaagcaaatg tacgtctgcc gaacggtcct ctgaaagccg ttggcgagta
     6721 cgaagtggaa gtggctttgc acactgatgc cgttgctaaa attaccgttg ccgtcgttgc
     6781 cgcaaccgag taattgaaat tatcagatgc cgtctgaaac cctgccgatt gtcggaatgt
     6841 ggtttcagac ggtatttgtt ttgtttttcg tatattctgc aaaggtctcg gtcaggttgt
     6901 ctgtcctttt gtcggaacag gagactgggc agctttgtcc tgattaattg cttgtgcaaa
     6961 cggtaaaaag gttttttcgt gtgttttaaa gcaggttgta cagcattttt ccggcaatca
     7021 aaagcaacat aatgccgaag gattttttga gtttggcaga agaaagtttg tgggcggttt
     7081 tgacaccgag cggggcaaag gcaatggttg ccgcgctgag gacggcgacg gcgggcaggt
     7141 aaaggaagcc cagcgaccct tcgggcaatc ctgcaatatt caggccgttg agcagatacg
     7201 atattgcgcc ggagagtgca atcggccagg caaggccgga tgatgtgccg atggctttat
     7261 gggcggggaa gccgcagtgg attaagaagg ggacggaaag tgaaccgccg cctatgccga
     7321 cccagctcga cattgcgccg aacagtgtgg aaaccgcagt cagtccgggc agcttgggca
     7381 gcgggcggga tgccgtctga gggccggtat gcagtgtttt gaatgcgacg acagttaaaa
     7441 acaggatgaa gaagatttga agcccgaatg cggggatata ttttgcggag agtgcgcccg
     7501 cgaatacgcc gaatatcata cccggcatca tcgcaaatac ggttttccag tcgaccgctc
     7561 gttttttgtg ctgccccaac atactggaaa aggcggtgaa gaccatgatg gcgaaggatg
     7621 tgccgacggc gaggtgttgc gcgtaaggat gttgtgccaa accctgcaaa tcaagcaccc
     7681 ataaaacgac agggacaatc agcgtgccgc cgcctacgcc gaacaggccg gcgataaaac
     7741 ctgccgcact gcctacggca agcaggatta agataatgtc ccaatgccac atttaatccg
     7801 cctttctgta ttttttccag aatcggcgtg ccgccataat gtagccggac aggctgtatc
     7861 cgagaaagaa caggaagagg acgagcgacg gttcccaagt gaccagaagc agcagtaaga
     7921 cggcaagcag cattccgaca aaggggactt gtctgcggat gttgatttct ttaaaactcc
     7981 aaaaagggat ttggacaatc attgacaggc cggcaaacag tgtgatgccc aatgcccacc
     8041 agtggacggc ggggaatttt tcgacgctgt ggttgaccca aatcagcccg acaatcagcg
     8101 cggcggcagt cggactgggc acgccgataa accagcgttt gtccaccttg ccgatgagcg
     8161 tgttgaacag ggcgaggcgc agggctgcgc aggcgcagta gatgaaggcg acggaataac
     8221 cgattttgcc gaactgccaa agctgccatt tgtaggcaat aagcgcgggg gcaacgccga
     8281 agctgaccat gtcggcaagg ctgtcgagct gctccccgaa cgcgctttgg ctgttggtca
     8341 gccgcgccac gcgcccgtcc ataccgtcca aaagcatgga aatgaatacg gcaatagcgg
     8401 cggtttcata acgtccgtgc atggattggg tgattgcgta aaacgcggaa aacagcgcgg
     8461 cgatagtaaa ggaattgggc agcaggtaga tactgttttg acgcagggag cgttgcgggg
     8521 tgttggtcgg ggtttccata gctgaagtgt ctcacggacg tggagaatcg ataggggatt
     8581 ataacccaaa ccattatcga acaatacgcc ctttaggatg aagccctcgg aggcggtttg
     8641 tttttctgat ggcggatgct ttgcggggcg gtttgactct gcctgccgcc gggtgtgttg
     8701 tttcggattt ttgccggatg gatgttgttg ccgggcattt ctgggcttgc cggaaggtat
     8761 cggggggagg gcatcggaat atgcggcgcg ccgttcaaac tggggcgacg tttccctatt
     8821 ttcagggggt tgcaggtttc aacaaattga aagatatttt ttgcatttcc gaccgtatgc
     8881 tgattttgaa acgggataaa ttataggaca aggtttttcg cccgccgcaa aggttggccg
     8941 caatgcagtc tgaaaacaac ggcttgccgc taaggcggcg gtatgcggat ttgcgctgcc
     9001 gaaaccggtt tgccataccc ggcggtgcaa tctgcgggga gggataagcc gataaggcgg
     9061 gacttatcct gttttttgtt tataatgcaa cagaatttgg acgaaccgtc gggaatttat
     9121 catgatgaaa aagatactgg ccgtatcggc actatgcctg atgactgcgg cggcacgggc
     9181 tgccgatact tacggctatc tcgccgtttg gcagaatccg cagaatgcgg acgatgtttt
     9241 gcaggttaaa actacaaaag aagattcgac gaaaagcgaa gcgtttgccg agttggaagc
     9301 cttttgcaaa ggtcaggaca cgcttgcggg cattgccgaa gacgagccga ccggatgccg
     9361 gtcggtcgtg tcgctgaaca atacctgtgt cgcgctggca tacccgaaag ccttgggcgt
     9421 gatgcgcgtt gaaaacgccg tcgtgattac ttctccgcgt tttacgagcg ttcatcaggt
     9481 cgcactcaac cagtgcatca aaaaatacgg cgtacaggga caatgcggct tggaaacagt
     9541 gtattgcaca tcttcttctt attacggcgg aactgtgcgc tctttgattc aaaatctcaa
     9601 ataaaacgga aaatgccgtc tgaaagatgt tcagacggca tttctatatc gacggtcagg
     9661 attctttcgg atcgggcagc aggctgttca acataatggc aagtacggcg cacaagccca
     9721 cgccggcaaa gctgaagctg cccaatttga gcgtcatgcc gccgatgccc gtggtcagta
     9781 ccgagctgac gatgaccagg tttttcggca gcatcaaatc gactttggca tcaatcagcg
     9841 ttttcacgcc caaagaagca atcgtgccga acagcagcag cataatgccg cccattactg
     9901 gcatcggaat ggaagccaaa aacgcattga atttgccgaa aaacgccatg cagacggcaa
     9961 aaaccgccgc ccaagtcatg atgacggggt tgctgttttt ggtaatcatc accgcacccg
    10021 ttacttcgcc gtaggtcgta accggcgggc cgccgatcag acccgcaacg catacgccca
    10081 aaccgtcgcc tgcaagggtt ttgtccaagc ccgggtcttt cgtatagtct ttccccgtca
    10141 cattgccgat tgccatgatg ccgccgatgt gttcgatggc gggggcgacg gcaacgggca
    10201 gcataaacag tgcagcctgc cagttgatct gaggcgtttc aaaatgggga acggcgaacc
    10261 agggcgcgtg tgcaatgctt gccgtgtcca ccagtcccat cagcagtgcc aaaacataac
    10321 ccgaagcgac accgatcaag atgggaatca gcttcatcat cctgctgccg aaaaccgata
    10381 cgatggcggt aacggcaaag gtaaagccgg aaagaatcag cgaatcggta tagtcgatga
    10441 cctgtttgcc gtccgcctga cccattgcca tgctgcttgc cgccacggca acagacagac
    10501 cgatgaccat gatgacgggg ccgatgacga ccggaggcag cagtttgtgt accgctgcca
    10561 gtccgcgcca acggatcagc gcggcaaaca caaaatacat aaagccggcg gcaaacagtc
    10621 cgaacatggt ggaaggcagc ccccattcgc cgacggagta gataatcggt gcgataaagg
    10681 caaacgaaga accaagaaaa atcggcactt tgcgtttggt tgtgatttgg aacagcagcg
    10741 ttcccaagcc tgcgcccaaa agcgcaagag ccggattcag accggtcagc aggggaacca
    10801 gcaccattgc gccgaatgcc acaaataaaa tctgtgcacc ggaaacggca agtttcagtt
    10861 ggttcatacc atcttctttc aatcggttca aacggcacgg attatactgg tttgccgagg
    10921 gtctgcgtaa aaatacatac gtcgggcaac agatatgccg tctgaaaggc aatgtctgtc
    10981 tgttgcagga tgaaagcagg agaaatggag tataatccga cactttccat attcaatccg
    11041 caagccgtcc ggcttcctta tttcagacgg cattttccgg taatacggca tgacagaaaa
    11101 atcccataaa aaaacagcaa aaggcagggc gggaagcccg tccccaacgt ccgcacgcaa
    11161 caagaaagcc gacaacggcg cacggggcaa taaagtttcc gagcggctca aggcggtcaa
    11221 agagttgcag aaaaccgaaa ccaaaaaggc gcgtcccgaa catgtcgtca accttatcgg
    11281 cgacgcactg tggctgatgg gtttggcggc aaccctgtat ttggcgattt ccctgatcag
    11341 tttcgatatg ggcgatccgt cttggtcgca cagttcgccg gttgtggaag atgtcgccaa
    11401 ttggggcgga ctgttcggcg cgtatgttgc cgatgtcggc tattatcttt tcggctggtc
    11461 gttctggtgg tggatagcgg ctgcctgcgt cgtgctgtat aaaaatttcc gcctgcacgc
    11521 aaaacagacg gaaaacgagg catacaacca caaaatcgct gccgccgcgc tgtttgtcct
    11581 gacggtcttc agccccgtct tggagtattt tgtgctgggc ggaaaatatg ccgactccct
    11641 gcctgtcgga gcaggcggta tggtcggcat acgcgtcggc gcagtgtttg cgtggctgct
    11701 ggggaaatcg ggcagcctgc tgattatctt ggttgttctg ctgttgtcgt tgtccctgct
    11761 ggtgcagatt tcatggctgg aatttttgaa cggtgcgggc agggcggttc aaaaccgcct
    11821 gagtgcctta tccggcaagg tcatggcttt aggaaaacgc cggccgaata ccaaaacaga
    11881 cggtgtcgat acccaaaata cacggcgcat ggtaaaagaa gccaagaata ttacggccaa
    11941 acccgttgcc ttgcccgaag gcagcagcag caaccgcaaa tccgtcgcgg tttccgtcgc
    12001 gccgccgccc aaaattcagg tttctctgtt tgaagatgac gaacctcggc aggcgggcga
    12061 ataccacaag cctacattga acctattgcg gattcctgac agcgaacccg tcagcatcaa
    12121 tcccgccgaa ttggagcgca ctgccgaact gatcgaatcc aaactggcag aattcggcat
    12181 cggcgtacaa gtcgtatccg ccacatccgg ccccgtcatc acgcgctacg aaatcgaacc
    12241 cgcgcaaggt gttaaaggca gccaaattgt tgccttgtcg aaagatttgg cacgctctat
    12301 gtcgctgcag tccgtgcgta tcgtcgaaac catcgcaggt aaaaacacga tgggcatcga
    12361 gttgcccaac gacaaacgcc aagacgtgat gttgagtgaa atcttgtcct cgcccgtgtt
    12421 tgccgaagcc aaatccaagc tgaccgtcgc gctgggcaaa gacattgccg gtacccccgt
    12481 tgtcggcgac ttggcgaaaa tgccgcacct tttggtcgcc ggtatgactg gttcgggcaa
    12541 gtccgtcggc gtgaacggca tgattatgtc tatgcttttc aaagctacgc ccgacgaagt
    12601 ccgcttcatt atgatagacc cgaaaatgct cgagttgagc atttacgacg gtattccgca
    12661 cctgctctgt cccgtcgtga ccgatatgcg cgaagcaggg caggcgttga actggtgcgt
    12721 cgccgaaatg gaaaaacgct accgcctgct ttcccatgcc ggtgtgcgta atttggaggg
    12781 cttcaaccaa aaagtcgaag ccgcaaaagc ggcaggcaag ccgctgctca atccgttcag
    12841 cctgaacccc gacgagcccg agccgctgga aaaactgccg ttgattgtgg tcgttatcga
    12901 cgaacttgcc gacctgatga tgaccgaacg caaagccgtc gagcagcaaa tcgcccgtct
    12961 cgcccaaaaa gcgcgcgccg ccggtatcca tatgattgtc gccacccaac gtcccagtgt
    13021 cgatgtcgtt accggcctga ttaaagccaa catcccgacg cgtatggcgt ttaccgtgca
    13081 aagcaaaatc gacagccgta ccatcctcga ccaaatgggc gcggacgaac tgctcaaata
    13141 tggcgattcg ctgttcctcc agcccggcag tgccgaaccg actcgcctgc aaggcgcgtt
    13201 tgtttcagac gacgaagtac atcaagtcgt caactacgtc aaatcgcaag ccccagccga
    13261 ctatattgaa ggtctgctca gcggcgaggc cgcgctggaa actgccaata tcgttaatcc
    13321 gaatgcagac agcgacgaat tgttcgatca ggcagtcgcc tatgttttgg aaagcaaaaa
    13381 aacctccatt tcgtctttgc agcggcagct gcgcatcggc tataaccgcg cggcaaacct
    13441 gatggaggca ctggaaaatg cgggtgtcgt ttcttccacc gacctcaacg gcagccgtaa
    13501 aattttggcg cacaaggacc atttgtagcc cgtattgcaa aatgccgtct gaacggcgga
    13561 attggcgttt cagacggcat attatgtttc aggcgaaaca ttgtgatata cttgccagct
    13621 aaaatttccc ctttgcggca atgcggttca aatatcgtac cgttgcgctg tttgcttccc
    13681 catgtaggga agaaagttta tcattttatc aacacaacaa atttaagggc ttatgatgag
    13741 cgtaactgtt gaaactttag aaaatctgga acgcaaagta gtgttgtccc tgccttggtc
    13801 cgaaatcaac gcagaaaccg ataaaaaact gaaacaaacc caacgccgtg caaaaatcga
    13861 cggtttccgt ccgggtaaag cacctttaaa aatgattgcc caaatgtacg gtgcgagcgc
    13921 acaaaacgac gtgatcaacg agctggtgca acgccgcttc tacgatgttg ccgttgccca
    13981 agagttgaaa gtggcaggct tcccccgttt tgaaggcgtt gaagaacaag acgataaaga
    14041 gtctttcaaa gttgccgcca tttttgaagt gttccccgaa gtcgttatcg gcgatttgtc
    14101 tgcacaagaa gttgaaaaag taaccgcttc cgtcggtgat gccgaagtgg accaaaccgt
    14161 agaaatcctg cgcaaacaac gcacccgctt caaccatgtc gaacgcgaag cccgaaacgg
    14221 cgaccgcgtc atcattgact ttgaaggcaa aatcgacggc gaaccttttg ccggcggcgc
    14281 atccaaaaac tacgccttcg tattgggcgc aagtcaaatg ctgcctgaat ttgaagccgg
    14341 cgtagtcggc atgaaggctg gcgaaagtaa agacgttacc gtcaatttcc ctgaagacta
    14401 ccacggtaaa gacgttgccg gtaaaactgc cgtgttcacc attacgctga acaacgtttc
    14461 cgaagcgact ctgcctgaag tcgatgcaga ttttgcaaaa gccttgggta ttgcggatgg
    14521 cgacgttgcc aaaatgcgcg aagaagtgca gaaaaacgta agccgcgaag tggaacgccg
    14581 cgtaaacgaa caaaccaaag aatccgtaat gaacgcgctg ctcaaagccg tagagctgaa
    14641 agcacctgtt gctttggtca atgaagaagc cgcacgcttg gcaaacgaaa tgaaacaaaa
    14701 ttttgttaac caaggtatgg ctgatgctgc caacttggat ctgcctttgg atatgttcaa
    14761 agaacaagcc gaacgccgcg tatctttagg tctgatttta gccaaactgg ttgacgaaaa
    14821 caaactggaa ccgactgaag agcaaatcaa agccgttgtt gccaactttg cagaaagcta
    14881 cgaagatcct caagaagtga ttgactggta ctacgcagat ccttcccgcc tgcaagcccc
    14941 gacttctttg gcggtagaaa gcaacgtcgt tgatttcgtt ttgggcaaag ccaaagtaaa
    15001 tgaaaaagct ttgtcttttg acgaagtgat gggcgcgcaa gcctgattat cctgaaaatg
    15061 ccgtctgaag gcagtttttg aaagcaccga agccgctttt aaagctcgct ttggtgcttt
    15121 tccatcatga aaggagacga aatgtctttt gataactatc ttgtccctac cgttatcgag
    15181 cagagcggtc gcggtgagcg tgcattcgat atctattccc ggcttttgaa agagcgcatc
    15241 gtattcttgg tcggaccggt aaccgacgag tccgccaatc tggtggttgc ccaactgttg
    15301 tttttggaaa gtgagaatcc ggataaggat attttcttct atattaactc gccgggcggt
    15361 tcggtaacgg ccggtatgtc gatttacgac accatgaatt tcatcaagcc cgatgtatcg
    15421 actttgtgct tggggcaggc ggcaagtatg ggcgcgttct tattgtcggc aggcgagaaa
    15481 ggcaaacgtt ttgccctacc caacagccgg attatgattc accagccttt aatcagcggc
    15541 ggtctgggcg gtcaggcatc cgacattgaa attcacgcac gcgaactttt aaaaatcaaa
    15601 gaaaaactca accgcctgat ggcgaaacat tgcgaccgcg atttggcaga tttggaacgc
    15661 gacaccgacc gtgataattt catgtctgcc gaagaagcca aagaatatgg tttgattgac
    15721 cagattttgg aaaaccgcgc ttctttgcgg ctttaataaa agaaacctga gagaaaccga
    15781 tgccgtctga aacgttcaga cggcatttgt ttcaagctgc gtttgatatg gtatcgatac
    15841 cgtcatccac gggacggaag aaggatttgg agttttttaa aggatccccg ttcccgcctg
    15901 tgatggacgg atgccgtctg aaagccaaat ggggcagcag tttttgcaag ggcgcgacac
    15961 tcgcatcctc ctgaatctgc cggtacggtt attgagaaaa gacaagggca ggaagcggta
    16021 tcccgccttc cttatttata cctgcaattc atctccgtat ttgccgattg tattttcata
    16081 ttgcgcctcc attccttatc ggtttaaaaa aatgcctttt ccaatgggat gggttttata
    16141 tcaatgaaaa taaacggttt ttatttaaaa atagccaagg taggctggcg gttggatttt
    16201 atagggagtg tatcgggata accttccctt gatggcgttt gagtccgttc tccccataat
    16261 tggttttgac atactcttca acaatagcca gaaattccgt tgcaatgttg tcgcctttca
    16321 gcgttacttt acgttcacca tctacataaa caggggcaac gggtgtttct cccgtaccgg
    16381 gcaggctgat gccgatgtcg gccaatttgc tttctccggg cccattgaca acgcagccca
    16441 ttacggcaac gttcagggat tcaaccccag gataaagggt acgccatata gacatttttt
    16501 ggcgcaggta attttgaaca tcttgtgcca gctcttgaaa tacggtactg gtggtacgcc
    16561 cgcagccggg gcaggcggta accatcggcg taaacgaacg caatcccata gtctgtaaaa
    16621 tctcttgccc gacgacgacc tcctgagtac gcgggctgcc aggttccgga gtcagtgaaa
    16681 tgcggatggt gtcgccgatt ccttcttgaa gcaagacgga taatgccgcc gttgatgcga
    16741 caatgccttt gctgcccata ccggcttcgg tcaaacccaa atgcagcgga taggcgcaac
    16801 ggctgcccag ttcgcggtaa acctgaatca aatcctgaac cgcgctgact ttgcacgaca
    16861 ggatgatttt gtcttcgggc agtcccaata gaacggcttt ttcggcagat tccaaagagg
    16921 agacaatcag tgcttccttc gtcacttctt cgggcggttt cggcgcggaa gaagcgaggt
    16981 tggcatccat catacgtttg gcgaggcttt gatccaaaga accccagttt acgccgatgc
    17041 ggacggcttt atcgttttca gcagcagtcc gaatcataaa ggcaaatttt tcatcgcctt
    17101 ttacgccttt gccgacattg ccgggattga tgcggtattt ggacaatgct ttgccgcatt
    17161 cgggaaattc cgccaacagg cgttcgccgt tgaagtggaa atcgccgata agcggtgtgg
    17221 catagcccat atcgtccaag cggcggcgga tttcggcaac tttggacgcg gcttcggggc
    17281 tgttgacggt aatacgcacc atttcggatc cggcatcgct caattcctta atctgcaatg
    17341 cggtggcttt tgcatcggca gtgtcggtgt tggtcataga ttggataacg acgggtgctt
    17401 ctgaaccgac ggtaatatga tcgatgcgga cttgatgcgt cttgcggcgt tggagtgtgt
    17461 tcatatggtt tgaatctgtt tattgaccgg tgaggacggt ttgcaattct tccgagtagg
    17521 ggaaattcgc ctgcaattgt gcttcatatt cgtatgccgc ctgtgcgttg ccgagggctt
    17581 tggcaatttt ccagcctagc agcaaatcat cggcctgaag gacttctacc ctgctttggt
    17641 attttttaaa gtagtaatcg gcatcgccca actgcccggc cagcattttg gtgcgcgcca
    17701 gttctttaaa tgcgggtggg aactgcggct gggcggcgag ggaacgtttc aaataggctt
    17761 ccgccaatcc gaattgcccc tgttttgcgc tgcatatgcc tttattcagg ttggcaatat
    17821 aaggggtcgg gtaggtgggg tcggccaggg ctttgtcgaa atatgccata gattcggcag
    17881 ggcggttgag cctgccgcac aggaaccagc cgtagttgtt gttgatttcg gcactgtcgg
    17941 gtttgatgga gagggcttgc cggaaacttt cctgcgcctt gtcgttaact ttcaggtatt
    18001 gatagatttc ggcacggacc agccaggcaa gctcgttttt agggtctgat ttcaaggcgt
    18061 cttcaatact tgccgtcgcc tgacggtagt cctgaccgcg catatattcc attgccaact
    18121 gggttttgat attggaaacc tgattggctt tttctgcccg cgaggggcgg taggaagtgc
    18181 tgcacgcgcc caaggcaagg acaagtaata aagagattcg tttggatggc ttaaaaggca
    18241 taattacccc tgttgtccga ttaaaatctg ctgccatttt tgttggcggc gcgttttatc
    18301 ctgaacctgc cccgccaact gtccgcaggc ggcatcgatg tcgtcgccgc gcgtttttcg
    18361 tacggtaacg acaaatcctg cctgctgcaa aatatcgcgg aacacacgga tgttctcatt
    18421 gctggagcgt tcgtatccgg agtttggaaa gggattgaac ggaatcagat tgaacttgca
    18481 gggaacatct ttgaccagtt cgatcagttc gcgcgcatgt tgcgccttat cgtttattcc
    18541 gtccaacatg acgtattcga aagtgatgaa atccctgggt gctttgacca gatagcgttg
    18601 gcatgcagcc atcaattctt tcaagggata ttttttgttc aacggtacga tttggttgcg
    18661 gacttcgtca ttggaagcgt gaagggaaac cgccaaagcc accggcatga catcgcgcaa
    18721 cctgtccatt tggggaacca tacccgaagt ggaaacggtt acacggcggc ggctcaaacc
    18781 gtagccgtgg tcgtccagca taatgcttaa ggcggtaacg acattgtcga agttcgccat
    18841 cggctcgccc atacccatca tcactacatt ggaaattacg cgttcgtttt tcggtgtaac
    18901 acccatcgct ttgtttgccc accacaactg tccgatgatt tcggcagcag tcaggttgcg
    18961 gttgaagccc tgccggccgg ttgagcaaaa tgtacattcc aaagcgcagc cgacttgcga
    19021 ggaaatgcag agcgtgccgc gatccgattc ggggatgaag acggtttcca ccccgttccc
    19081 cgtcccgaca tctagtagcc attttcgagt gccgtctgaa gatttttgag acatcatcag
    19141 cttgggaatt tcgattcctg cctgttcgtt cagtttatgg cgcaacgatt ttgccaaatc
    19201 ggtcatttcg tcaaaatttt gcgcgccgga ttggtgcatc caacgcataa cctgtttggc
    19261 acggaaaggt ttttcgccca tatcggcaaa atgtcgggtc agtccttgaa ggtcgtagtt
    19321 gagcagattg gttttcatgt gtttgtgttt ttcttaaatt aaggcttcaa ataaaaaatt
    19381 gcagggcagg caagtttgat ttgaagcctt ttgcgacaga gttttcagac ggcatggata
    19441 tatttatgcc gtctgaagag gagttgtgcg gtgtattgta tcaacggggg cagatttcgg
    19501 tttggctgaa aaagtaggca atttccaaag cggcattttc cacgctgtcg gaaccgtgta
    19561 cggcattaat gctgaccgaa gtggcaaagt ccgcgcgtat cgtgccttcg gcggcttcgg
    19621 aaggattagt tgcacccatc agttcgcggt ttttcaggac ggcgttttca ccctctaata
    19681 cctgaatcat aaccggaccg ccggtcataa attcaaccaa tccggcgtag aaggggcggt
    19741 ctttatgaac cgcataaaat tcttgcgcct ctttgagggt aagctgcttc attttggcgg
    19801 caacgatttt cagaccgttc tcctcaaagc ggctgtatat tttgccgata acgtttttgc
    19861 cgacggcatc gggtttgatg atggagatgg tacgttcaat cgccatgcta tatccttatt
    19921 tttacttaag aagaatcaaa tcggtattct atcaaaaaaa taacttatcc gcctgaagcc
    19981 ttgggagata gggtcaggtg cggctgttcc aaataaactt cagactgcat ttcgaccatc
    20041 cggctggcgg tgcgggtaaa ttcttcggca aaatcgcctt ccgtatagat atggttgaca
    20101 tctaccgcgc cggtggcaca cagtttgacc cggaaatcgt agagtacgtc aatcagccaa
    20161 gtcagccgcc gcgcctccgc cttttcttgc ggtgagagtt gttccaaacc tgaaataaaa
    20221 accatttcat aatgttcggt caaatacaga tagtcggact gtgagcgggg gccgaagcac
    20281 agtgcgcgga aatcaaacca tatggcacgg ccggactcgg ctttgtgggg aatctcccga
    20341 ccgtggatgg tgctgatgcc ggggttcaaa tcggtaatgc ctgtcatttc tttgaacagt
    20401 tttgccagtt ttgcctcatt ttcttcattg gcaggcgtaa agaaaatctc ggcggggcgg
    20461 agggtacgca gtcggtagtc ttcaccgccg tcaacgttta agacggtcag gctggactcg
    20521 atgagcgcga ttgtgggaag aaaactgctc cggttttgac cttgcgggta gagttcggaa
    20581 ggcgcgtagt ttgaagtcgc caccaaaaca acgccctcgt taagcaggtt ttccagcaga
    20641 cggcctaaaa tcattgcatc cgcaatatcg ctgacatgaa attcgtcaaa acataatacg
    20701 cgggtttctt tggcaatctc ggcggcaacg gatttcaacg ggttgctttc gcttttcagg
    20761 gttttcagcc tctggtggat ttctgccata aaggcatgaa agtggacgcg gcgtttgcgg
    20821 cggtaaggga ggcagccgaa aaaagcgtcc atcagaaagc ttttgccgcg tccgaccccg
    20881 ccatagaaat aaagcccttt ggggacttgc ggggaacgca aactcctgcc taaaaaacgg
    20941 tttcttttgc gtttgaacat catcaattcg gtccaaagcc gatcgaggtg ttcgatggcg
    21001 gctgcctgcg cgtcgtcgcg gatgaagttg ggcagttgtg aggcagcctg ataccaagtc
    21061 agcgggctgt gattttcaaa cggcggggct ttaaaaagtt ggtctctatt actcatattt
    21121 aaccttgtta tacttcttgc ggctaagaaa gaaaatgctt accgtcggta ttataaatga
    21181 tttccttcaa acttggcatt ttccgtggaa aagaataatg acgcgcacaa gggggtataa
    21241 gcaaaggcgg cgtttcaata tggtgcagat atgagctcaa atgcctttta tagt
//
LOCUS       DAXHNE010000018        21273 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.018, whole genome shotgun sequence.
ACCESSION   DAXHNE010000018 DAXHNE010000000
VERSION     DAXHNE010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21273
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.018"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(97..1116)
                     /gene="lepB"
                     /locus_tag="ACFM4Z_000527"
     CDS             complement(97..1116)
                     /gene="lepB"
                     /locus_tag="ACFM4Z_000527"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFC6020615.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFAAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNIFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRDKILTVNGKPTSDIPNGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDSSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(1259..3052)
                     /gene="lepA"
                     /locus_tag="ACFM4Z_000528"
     CDS             complement(1259..3052)
                     /gene="lepA"
                     /locus_tag="ACFM4Z_000528"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFC6020616.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPAGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            complement(3195..3896)
                     /locus_tag="ACFM4Z_000529"
     CDS             complement(3195..3896)
                     /locus_tag="ACFM4Z_000529"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFC6020617.1"
                     /translation="MTLKTVAVIGAMEQEIELLRETMENVKAVSFGKFAAYEGELAGK
                     RMVLALSGIGKVNAAVATAWLIHQFAPECVINTGSAGGLGKGLKVGDVVVGTEIAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDGLLVGKAKQAAEVFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPKVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            4064..5194
                     /locus_tag="ACFM4Z_000530"
     CDS             4064..5194
                     /locus_tag="ACFM4Z_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC6020618.1"
                     /translation="MFTDENMTEKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKI
                     TRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGAT
                     ALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
                     SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDR
                     ETMDYAIAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQ
                     RLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQL
                     YEKGDISLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            5228..6205
                     /locus_tag="ACFM4Z_000531"
     CDS             5228..6205
                     /locus_tag="ACFM4Z_000531"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFC6020619.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQYMKPVYYPAYEDRLLQ
                     TVSGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            6209..6559
                     /locus_tag="ACFM4Z_000532"
     CDS             6209..6559
                     /locus_tag="ACFM4Z_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HFC6020620.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            6564..7343
                     /locus_tag="ACFM4Z_000533"
     CDS             6564..7343
                     /locus_tag="ACFM4Z_000533"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC6020621.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     AEVSAIAEVYEHIYCTIGVHPDSKEAEEFSIAEMVAAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRTV"
     gene            7369..7584
                     /locus_tag="ACFM4Z_000534"
     CDS             7369..7584
                     /locus_tag="ACFM4Z_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFC6020622.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREAKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQY"
     gene            7685..7996
                     /gene="grxD"
                     /locus_tag="ACFM4Z_000535"
     CDS             7685..7996
                     /gene="grxD"
                     /locus_tag="ACFM4Z_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HFC6020623.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            8102..8728
                     /gene="upp"
                     /locus_tag="ACFM4Z_000536"
     CDS             8102..8728
                     /gene="upp"
                     /locus_tag="ACFM4Z_000536"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006223 - uracil salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFC6020624.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLATELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPISYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            8751..9071
                     /locus_tag="ACFM4Z_000537"
     CDS             8751..9071
                     /locus_tag="ACFM4Z_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HFC6020625.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLGKEAAEQGLIWFAEHVADARAHPGKHPNIDLLENVVQSGETWLLKP
                     LSAQ"
     gene            9322..9741
                     /locus_tag="ACFM4Z_000538"
     CDS             9322..9741
                     /locus_tag="ACFM4Z_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Gly1ORF1"
                     /protein_id="HFC6020626.1"
                     /translation="MKKMFLSAVLLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRWATMKFQNGEFMYTIWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFS"
     gene            9762..10523
                     /locus_tag="ACFM4Z_000539"
     CDS             9762..10523
                     /locus_tag="ACFM4Z_000539"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFC6020627.1"
                     /translation="METAKPVMLIVRPSGRAAEDVETCLNAGWRAEVLSPVEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKAQIAVGQGSRRALERCLVRTV
                     IAPDDGNDSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            10598..11872
                     /locus_tag="ACFM4Z_000540"
     CDS             10598..11872
                     /locus_tag="ACFM4Z_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFC6020628.1"
                     /translation="MPSETSSPRKENETEVHIPAAPFIVKQSGSNALAVCALVLAALG
                     LGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAALLKDNLNRQAAIQSELDRLD
                     GNVKANGEQILEMQKSYRELTKGRADWLVDETETILNLAAQQLVLTGNIQTAVGVLEH
                     IDSRLSRFNQAELLPIKQAVSSDLAELKNRPYVDISGTALRLDRLETAVSGLPLMLDG
                     VLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRLENNDAMLISPEQAYFVRENL
                     RLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKSPATQSWLKELAELKALDVRM
                     TADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPANEKTASEPAAASDVKTIEAP
                     SLPSERKPEQPAKKQTVPEKAGRSPSAKGERA"
     gene            11869..13092
                     /locus_tag="ACFM4Z_000541"
     CDS             11869..13092
                     /locus_tag="ACFM4Z_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HFC6020629.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVLNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDEIGEPQKAEAQRNLVLE
                     AVSDDERHAALEQHS"
     gene            13125..13565
                     /locus_tag="ACFM4Z_000542"
     CDS             13125..13565
                     /locus_tag="ACFM4Z_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003778183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020630.1"
                     /translation="MGSSDSFKEKKEIFEIGTPAYRQKLIDVWKKSINGNEKSWVLFE
                     NGTCVILLEPEKDLAKQAKEMLSKWGKVQIGTPSADFGIITLDSGDGYAVSCHHPEIF
                     TLILKEEGLDEDFKIGIEGRSHRDCDAEEPKVIHIEDKRTIETP"
     gene            13719..14783
                     /gene="hemE"
                     /locus_tag="ACFM4Z_000543"
     CDS             13719..14783
                     /gene="hemE"
                     /locus_tag="ACFM4Z_000543"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFC6020631.1"
                     /translation="MISLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEADIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            14969..16348
                     /gene="radA"
                     /locus_tag="ACFM4Z_000544"
     CDS             14969..16348
                     /gene="radA"
                     /locus_tag="ACFM4Z_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFC6020632.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQTA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPLPEKMVAF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(16623..17102)
                     /locus_tag="ACFM4Z_000545"
     CDS             complement(16623..17102)
                     /locus_tag="ACFM4Z_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HFC6020633.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPQTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAAHRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(17234..17593)
                     /locus_tag="ACFM4Z_000546"
     CDS             complement(17234..17593)
                     /locus_tag="ACFM4Z_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC6020634.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHSAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            complement(17632..21246)
                     /gene="recB"
                     /locus_tag="ACFM4Z_000547"
     CDS             complement(17632..21246)
                     /gene="recB"
                     /locus_tag="ACFM4Z_000547"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFC6020635.1"
                     /translation="MSASIQAFDPLTVPIAGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAELGDGALSDGIAAYCAE
                     HHKGDTFLPTLLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLVPAQDFWRERVSNDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTVCRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFGYD
                     LNALEEAEEATIIRLQLDLIGYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPERPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAAGGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNGGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRQTYAQEKDGITMLKQNWRRVADNAPAGTDFAFTENAPPPAAYRSNAGQTAE
                     FAANSIPERRFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVAAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEMWLPAVAEM
                     AEACQKTPLTGAYCLSDIPPECRCPEMGFTLHTEDFGLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPEGNICVIDYKSNHLGADASAYTRQAMDEAVAHH
                     HYYLQALIYAVASARYFKLRGQPPAAVSVRYLFLRGIDGKGGGVWHWDIDAAALEQIK
                     "
BASE COUNT         4888 a         5495 c         5962 g         4928 t
ORIGIN      
        1 gtaggaaaat ccgacaacgt tagatccgcc tgcaaaagtg aggaatagca aatgccgtct
       61 gaaactattt tcagacggca ttcttggctt cctggcctaa cggattgccg taccggccct
      121 gccgaaatcg ccgaagttca tcaaaatgaa cattgccttg ccgacaacca gcttgtcatc
      181 cacaaatccc cagtagcgcg aatcggcact gttgtcgcgg ttgtcgccca tagcgaaata
      241 gcgtccttcg ggaactttgc acacgaaacc gctgctgtcg tcggcatatt ggcagtgttc
      301 caaaccgctt tgctctatgg aatatccgtt ttcagacata atatcggagg tatatttgcc
      361 caatacgggc agggaaacgg caggctgtcc ttcttttttc agaatattga aggatttgcc
      421 gtctagaccg ctgcggaaca tatccgtgtt gtggatttcg gaagggtcgg tgtcgtcggg
      481 ataacggtat gtgccgttag gaatatcgga agtgggtttg ccatttaccg tcaaaatctt
      541 atcccgatat tcgaccacat cgcccggaat gccgacaata cgcttgatgt aggtcatctc
      601 tggctgcaga ggataattaa aaacaacgac atcaccccgt tcgattttgc ctgtaggaat
      661 aaatatattg tttaaaacgg gtacgcgcag gccgtaggaa aatttgccga ccaaaatgaa
      721 atcgcccttg atcaggcccg ggcgcatcga gctggatgga atttggaacg gttcggcaat
      781 aaacgatcgg atgaggaaca ataccaaaac ggtagggaag aaactgccga aataatcgcc
      841 gaagtggctg ctttccgaga tttcgggatg agtcttcagg cggtatttat atacccccca
      901 agccgtaccg cacaatacaa cgaaaatcag gaaaacggcg gcaaagctca taaacaggga
      961 caaagcggca aacacgccga ccgccgtcag gatataggcg tattccagac cggaactcca
     1021 ttcaccgttt tcctgccgct ttttgtcgct tttgaaataa aggatgatgc cggcaagcag
     1081 cgcggcagcc gcgcccgaca ttagcattgt gttcattgtt gttccttaat gcttaaaaac
     1141 ccgcctgtcc gtgcaaccgt tttaaggcgg caaattgcaa aatttgtttg cgggcgcgtg
     1201 cccctgaaat cagggcggtt tgaggggtgt tcccgacgcg ccgccctgtg tgccagagtt
     1261 atttgtcgct cacctgcaaa atcgccaaga acgcgctttg cggaatttcc acattgccca
     1321 cttgtttcat acggcgttta cctgcctttt gtttttcaag cagttttttc ttacgcgtaa
     1381 tatcgccgcc gtaacatttc gccaaaacgt ttttacgcag ggctttgacg ttttcgcggg
     1441 cgataatctg gctgccgatg gcggcttgga cggcgatgtc gaacatttgg cgcggaatca
     1501 gctcgcgcat ttttgatgcc agctcgcggc ctcggtgaac cgcgctttga cggtgcacaa
     1561 tcaggcttaa tgcatcgact ttttcgccgt tgaccataat atccagctta atcaaatcag
     1621 acggttggaa ctctttgaaa tggtagtcca acgaagcata accgcgcgaa gtggatttga
     1681 gtttgtcgaa gaagtccatg acgacttcgt tcatgggcaa atcgtaagtc agcatgactt
     1741 ggcggcccat gtactgcata ttgacctgaa cgccgcgctt ttggttacac aaagtcatga
     1801 cgttgccgac gtattcctgc ggcacaagga tggtcgcggt aataatcggc tcgagtatgg
     1861 tttcgatgct gccgatgtcg ggcagtttgg acgggttttc aacttcgatt ttttcgccgc
     1921 ttttcaacac gacttcgtaa accaccgtcg gcgcggtggt aattaaatcc atatcgaact
     1981 cgcgctccaa gcgttcctgc acgatttcca agtgcaacag acccaagaag ccgcaacgga
     2041 agccgaagcc caatgcctgg gaaacttcag gctcgaattt caacgaagcg tcgttaagct
     2101 gcaatttttc caaagcgtcg cgcaaggctt cgtagtcgtg gctttccacg ggataaagtc
     2161 cggcgaatac ttggctttgt acttcttgga agccgggcag cggctcagtg gcagggttgg
     2221 caaccagtgt aaccgtatcg ccgactttcg cctgacccaa ttcttttacg ccggtaatca
     2281 aaaagcccac ttcgccggct ttgagttctt gtttttgaac cgatttcggt gtgaatacgc
     2341 ccaactgctc aacctgcgtt tccgccttgg tgctcataaa gcgcactttg tctttcagtt
     2401 tgatggtgcc gtttttcact cgaatcagca tgaccacgcc gacgtagttg tcaaaccacg
     2461 aatcgacaat caccgcttgc agcggcgcgt tttcgtcgcc ggcaggtgcg gggattttgg
     2521 caacgatttc ttccaaaacg tcttccacgc cgatgccgct tttggcggaa cactgcaccg
     2581 cgccgaccgc atcgatgccg atgatgtctt caatttcctg ctccacgcgt tcggggtcgg
     2641 cggccggcag gtcgattttg ttcaaaacag gcacaacttc cacgcccaaa tcaatcgcgg
     2701 tatagcagtt cgccacagtt tgagcttcta caccttggga cgcatcgacc accaaaagcg
     2761 cgccttcgca agctgacagc gaacgggata cttcgtaaga gaagtcgacg tgtcccggcg
     2821 tgtcaatcaa attaagctga tacacctgcc cgtcgcgcgc tttatagttg agcgcggcgg
     2881 tttgcgcttt gatggtaatg ccgcgctctt tttcgatgtc catggaatcg agtacctgcg
     2941 tactcatttc gcgcaaatcc aaaccgccgc aatattggat gaagcggtcg gcaagcgtcg
     3001 atttgccgtg gtcgatgtgg gcaatgatgg agaaatttcg gatatttttc attagagttg
     3061 ttttgaatgt cggacagtgg gtttgggaaa tgccgtctga acaaacggcg ttgcgtccga
     3121 atatcgggtg caacgtggaa atagcccgat attctaacgg aaaaccgctg ttttggcatg
     3181 atttgataaa ggttttataa agatttgacg atttctgcca ccatttttgc agaacttgcc
     3241 gccgccgttt tcaaaaattc gtcaaagctg atgtctgcct tttcgtccgc cgaatcggaa
     3301 accgcgcgga tgatgacgaa aggcgtttcc aactgatgac aggtttgggc aatcgccgcc
     3361 gcttccattt ccactgcttt gactttgggg aagtgcttgc ggatttccgc cacgccttcg
     3421 ctgctgtgga cgaagcggtc gccgctgaca atcaggcctt gttctaccgc cgcgccttca
     3481 aacacttccg ccgcctgttt tgctttgccg accaagaggc cgtctgaaac aaatacggcg
     3541 ggcagttgcg gcacttgtcc ccaaacatag ccgaatgcgg ttacatcgac atcgtggtgc
     3601 gcgatttctg tgccgaccac gacatcgccg actttcaaac ccttgcccaa accgcccgcg
     3661 ctgccggtgt taatgacgca ttccggtgcg aattgatgga taagccaagc cgttgcaacc
     3721 gccgcgttga ccttgccgat gccgctcaat gcaagcacca tgcgttttcc cgccaattcg
     3781 ccttcataag cggcaaactt gccgaaagag acggctttga cattttccat cgtctcgcgc
     3841 aaaagctcga tttcctgttc cattgcgccg ataacggcta ctgttttcaa agtcatattg
     3901 ctgacctgct gtgaatttcg gatagaatgc cgaattatac acgctaacac ggcaggattg
     3961 agtggaggcg gtttgtccat gccgtctgaa acggtttcag acggcatggc gggtttttgg
     4021 tagaatggga aggtacagat tgtttgaaga ttgagggacg aggatgttta ccgatgaaaa
     4081 tatgaccgaa aaggaagaac tgttcgcatg gctgcgccat atgaacaaaa acaaaggttc
     4141 cgacctgttt gtgacgaccc atttcccgcc cgcaatgaag ctggacggca aaatcacccg
     4201 catcacggac gaaccgctga cggcggaaaa atgtatggaa atcgcctttt cgattatgag
     4261 tgcgaagcag gcggaagaat tttcatcgac caacgagtgc aacttcgcca tcagcctgcc
     4321 ggacaccagc cgcttccgcg tcaatgcgat gatacagcgc ggcgcgacgg cgttggtatt
     4381 ccgtgcgatt accagcaaga ttcccaagtt tgaaagcctg aacctgccgc cggtcttgaa
     4441 ggatgtcgcg ctgaaaaaac gcgggctggt tatttttgtc ggcggcaccg gctcgggcaa
     4501 atcgacttcg cttgcctcgc ttatcgacta ccgcaatgaa aattcgttcg gacacatcat
     4561 caccatcgaa gacccgatcg agtttgtcca cgaacacaaa aactgcatca tcacccagcg
     4621 cgaggtcggc gtggatacgg aaaactggat ggcggcgttg aaaaacacgc tgcgtcaggc
     4681 gccggatgtc atccttatcg gcgaaatccg cgaccgcgaa acaatggact acgccattgc
     4741 ctttgccgaa acggggcatt tgtgtatggc gacgctgcac gccaacagca ccaatcaggc
     4801 actcgaccgc atcatcaact ttttccccga ggagcggcgc gaacaattgc tgacggattt
     4861 gtcgctcaac cttcaggcgt ttatttcgca acgcctcgtt ccgcgagacg gcggcaaggg
     4921 cagggtggcg gcagtcgagg tgctgctcaa ttcgcccctg atttcggagt tgattcacaa
     4981 cggcaacatc catgaaatca aagaagtgat gaaaaaatcc actaccctgg gtatgcagac
     5041 cttcgatcaa cacctttacc aattgtatga aaaaggcgat atttccctgc aagaagcatt
     5101 gaaaaatgcc gattccgcac acgatttgcg tttggcggta cagttgcgca gccgccgcgc
     5161 gcaaagttcc agccccgatt tggaactgct ctgatggcgg tatggatttc cggacggatg
     5221 gtttgaaatg atttatccgt ggcataatga gcaatggcgg cagattgcgg aacattggga
     5281 gcgtcgtccc aatgcatggc tgtttgccgg caaaaaagat acggggaaaa ctacatttgc
     5341 ccgctttgcg gcgaaggcac tgttgtgcga aacccctgca ccgggctgca aaccctgtgg
     5401 cgaatgtatg tcctgccatc tgtttggaca gggaagccat cccgattttt acgaaatcac
     5461 ccccttgtcg gacgaacccg aaaacggacg caaactgttg cagatcaaaa tcgatgccgt
     5521 cagggaaatc atcgataatg tgtacctgac ttcggtacgg ggcggtttgc gcgtgattct
     5581 gattcatcct gcggaaagta tgaatgtcca agccgccaac agtttgttga aagtgttgga
     5641 agaaccgccg ccacaagtgg tctttttgct ggtcagccac gcggcggaca aggttttacc
     5701 gaccattaaa agtcgctgcc ggaagatggt tttgcccgct ccttcccatg aagaggcatt
     5761 ggcatatctg cgtgaaaggg gtgtggcgga acctgaggaa cgtctggctt tccattccgg
     5821 agcgccgctg tttgatgagg cggacggtgt ccgtgcgttg cggattaaac tgttggatat
     5881 tttggcagaa ccaaggttgt tgaagatttt ggattacgcc gcgcttttcg ataaggagaa
     5941 acttccgctc gccgtatttg tcgggtggat gcagaaatgg ctggtcgatt tgggattgtg
     6001 cctgcaatac atgaaacccg tctattatcc cgcttatgaa gacaggctgc ttcagacggt
     6061 atccggtttc cgtccgcgca atgtatttgc ggcggaggat atgctcaaac agcttgcccc
     6121 ctacgggttt catactttaa atgtcaaaat gcagatcgag catctgctca tcaactattt
     6181 ggaattgaag aaagagaacg ggtgaattat gtcagacgga caaaatattc cggcaaaaat
     6241 gatgtcgttg cagctgaaag acatgaatct gctgtacagc tcctacatgc cgtttttgga
     6301 acacggcggt ctgtttgtgc agaccaacga cgtattttcc atcggggacg atattctgct
     6361 tgccgtagaa atcctcaact tccccaaact gttcctgccg accaaagtcg cctggatcaa
     6421 tcctgcgcgt acttcctcca aacccaaagg ggtggggctg gcattcacaa aacacgaaaa
     6481 ctgcctgaaa gtcaaagacc agatcgaagt cgaactgggc aacacaatcg gcggcagcag
     6541 acctacgttt accatgtaac gccatgcata tcatcgattc gcactgccac ctcaattttg
     6601 aaggtttgaa agaacgcctg cccgaagttt tgtccaacat ggaagcaaac ggcgtggggc
     6661 aggcgctcgc catcagcgtt agcagggaaa gttttgccga agtatccgct atcgccgaag
     6721 tttatgaaca tatttactgc acaataggcg tacatcccga cagcaaggaa gccgaagaat
     6781 tttctattgc ggaaatggtt gctgcagccg cccatccgaa agtggtcggc atcggcgaga
     6841 cgggtttgga ttattactgg tgcaaaggcg atttgtcctg gcaacacaaa cgctttgcag
     6901 accacatcga agcagccaat caaaccggac tgcccgttat cgtccatacg cgtgatgcgg
     6961 cggcggacac cttgtctatc ctgaaagaat gccgggttaa ttcgggcgtt atccactgtt
     7021 tttccgaaga catcggtttt gcccgtgcag caatggattt ggggctttat atttctttct
     7081 cgggaatcgt tacctttaaa aacgcaccct tggttcagga ggcggcgaaa tatgtgccgg
     7141 acgaccgcat tttggtggaa actgatgcgc cgttcctcgc ccccgttccc aaacgcggca
     7201 ggcagaacga accggctttt gtgcgccata ccgccgaaca tatcgccaaa ttgcggaacc
     7261 aaacattgga acaggttgcg gcatatacga cggaaaactt ttaccggctg tttaaaaaag
     7321 tacccgatat gcggaccgtc tgaccctgta ccgacgataa ggaaaaccat gaaggcaatt
     7381 catccgtatg catgtccgcg ctgctgccgg ctgcctgcca acacgtttcg gacaggcatg
     7441 gcaaattccg cttccaaatt ctgcattgcc aaaggcggca gacgggaagc aaaaaaagac
     7501 gaaagcggcg gcggatatgc cctgtgccat ttgccggaca gcaggattgt cgaggagtgg
     7561 gaatatttcc gttcacaata ttgatactgc gcgatatacg gcaaatattg tgggaagttt
     7621 ccgcttttgc gtataatgcg ccctacctga caaattttgt caactttatc aaaaggaata
     7681 agcgatggct tccatccacg accaaattaa agaagtagta acgacacacc gcgtcgtatt
     7741 gtttatgaaa ggtacgaagc agtttccgca atgcggtttc tcttcccgcg ccgtgcaaat
     7801 cctgaacgcg gcaggctgca ccgattacgt taccgtcaac gtattggaaa atcccgaagt
     7861 gcgccaaggc attaaggaat acagcgactg gccgaccatc ccccaacttt atgtgaacgg
     7921 cgagtttgtc ggcggttcgg acatcctgat ggaaatgtat gaggcaggcg agctgcaaga
     7981 gctgctgaaa gcctgatgga ttcggcaatg ccgtctgaac gtgtttcaga cggcattttc
     8041 ttttccggca aatcaaaaaa aagtataatg gcgcgtctca aaatcacatt ggaacaccgc
     8101 gatgaacgtt aatgttatca accatccgct cgtccgccac aaattaaccc tgatgaggga
     8161 ggcggattgc agcacctaca aattccggac gcttgccacc gagctggcgc gcctgatggc
     8221 atacgaggca agccgtgatt ttgaaatcga aaaatacctt atcgacggat ggtgcggtca
     8281 gattgaaggc gaccgcatca agggcaaaac attgaccgtc gttcccatac tgcgtgcagg
     8341 tttgggtatg cttgacggtg tgctcgacct gattccgact gccaaaatca gtgtagtcgg
     8401 actgcagcgc gacgaagaaa cgctgaagcc tatttcctat tttgagaaat ttgtggacag
     8461 tatggacgaa cgtccggctt tgattatcga tcctatgctg gcgacaggcg gttcgatggt
     8521 tgccaccatc gaccttttga aagccaaggg ctgcaaaaat atcaaggcac tggtgctggt
     8581 tgccgcgccc gagggtgtga aggcggtcaa cgacgcgcac cctgacgtta cgatttacac
     8641 cgccgcgctc gacagccact tgaacgagaa cggctacatc atccccggct tgggcgatgc
     8701 gggcgacaag attttcggca cgcgctaact gactgatttt cggagttgat atgaattttc
     8761 aagactatct cgccacattt ccttcaatcg accatctggg cggtttggac gttcaggatg
     8821 ccgacggcaa aacggttcac cacattcctg ccgttcaggg taagctcggt tcgctcaagc
     8881 tgtacaatgc tttggcggaa cgttttgacg gaaaattggg taaagaagcg gcagaacagg
     8941 gtttgatatg gtttgccgaa cacgttgccg acgcgcgtgc ccatccgggc aagcatccga
     9001 acatcgatct gctggaaaat gtcgtgcaaa gcggtgaaac ctggttgctc aagccgcttt
     9061 ccgcgcaata attttcgacc atgccgtctg aaatccgttt cagacggcat tttgtcggaa
     9121 agaagaccgt aaaacgggca ttttcttttc tatttcagga tacgggcaat gatgtttcaa
     9181 cacacaggac gacacataaa gcgccgccct atgtgttgcc ctaatttgga aggggttaca
     9241 cccttttcaa ataaaatctg atgctgctgc cacgaaggac ggatgtccga gtggcggggt
     9301 ttcaaccatt aaggaaatac gatgaaaaaa atgttccttt ctgccgtatt gcttctgtcg
     9361 gctgccgccc aaaccgtgtg ggcggatacg gtgttttcct gtaaaacgga caacaacaaa
     9421 tacatagaag tccaaaaaat caaccgcaat ctttacgaat attcgttcgg cagtgcggca
     9481 aaaaaagaaa ttgccatacg caacagcaaa gctgacctgt tggggcgttc cgacaggtgg
     9541 caaggtatgg gcagcggtcg ttgggcaacg atgaaattcc aaaacggcga atttatgtac
     9601 accatatgga caggcttcga ttccgtgact catacggaaa gcagcggtgt cgttgtggag
     9661 cgtaggggca aggaagtcgc acgggtcggc tgtacgccga aaaccgcgca ggcgaatttc
     9721 aacgatgacg atttttccta gtaatcgggg cggataaggc gatggaaaca gcgaaacccg
     9781 tcatgctgat tgtccgtccg tcgggcaggg ccgcagaaga tgtcgaaact tgcctgaatg
     9841 ccggttggcg cgcggaagta ttgagtccgg tcgaaatcga agcagatgct gccggactgg
     9901 aacttttgtc cgaacaatat gcccgtgcgg atgccgtgtt ttgggtcagt ccgaccgccg
     9961 ttgaaaccgc cgtcccgtac cttaaccttt cagacggcat aaaggcgcag attgccgtag
    10021 ggcagggcag ccgccgcgca ttggaacgct gtttggtcag aacggtcatc gcgcctgatg
    10081 acggcaacga cagcgaggcg gttttgcgcc tgccggtttg gaacagtctg cccgaaggtg
    10141 cgcgcgtatt gtttgtgcgc ggacacggcg ggcgggattt tttgatgaat gccttgcagg
    10201 agaaaggttt tcggacggag gtggcagaag tctatttcag acggcataaa cctttgaact
    10261 ttcaaaattt ccaaaccgaa aatattgccg ccgcctatat tacgtcgacc gagctggtgc
    10321 gcttgctgtt cgggcagctt ccgccgcaat tttcccgatt cttcaaatcc ttgctatact
    10381 ttacccatca tccgcgcatt gcggaggcat tgaagcgcga aggcgtgtgt tcggtcgaaa
    10441 ccgtccctac gctggaagcc gcgctttccc attcttccat ttccgtttca gacggcatgg
    10501 tctttcccgg aacctcaaat taataaggag caaaacggtg ggcgaacctg aaaacaaatc
    10561 atccgaaccc gtacgcgaga tacaggcatc aaaagaaatg ccgtctgaaa cctcttcccc
    10621 acgcaaagaa aacgaaacag aagtacacat tcctgccgct ccttttatcg tcaaacagtc
    10681 cggcagcaac gctttggcag tctgcgccct ggtattggcg gcattgggtt tgggtacaag
    10741 tggttttttg tttgtccaag gacagaatgt cttgaaaaac caagagctgg cattcaacca
    10801 aaaaatcgac aaagccgcct tgggcgagtc ggaaaacgcc gccctgttga aagacaacct
    10861 caaccggcaa gccgccatac aatcagagct cgaccgtttg gacggaaacg tcaaagcaaa
    10921 cggcgaacaa atcttggaaa tgcaaaaatc ctatcgcgag ttgaccaaag gacgcgccga
    10981 ttggctggtg gacgaaaccg agaccatact caatctggcg gcgcaacagc tggtgttgac
    11041 tggcaatatc caaacggcag tcggcgtatt ggagcatatc gacagccgcc tgtcccgttt
    11101 caatcaggca gagcttctgc cgatcaagca ggcggtcagc agcgacttgg cggaactgaa
    11161 aaaccgtccc tatgtcgata tttccggcac ggcattgcgc ctcgacaggc tggaaaccgc
    11221 cgtatccgga ctgccgctga tgctcgacgg cgtgctgaaa ccgggcgtac aggtgaagaa
    11281 cgaagccgct tccgcttcat ggtggcagaa cgtatgggaa aaatccctcg gcacattgaa
    11341 ggggctggtc gaaatccgac gtttggaaaa caacgatgcc atgctgattt ctcccgaaca
    11401 ggcatatttt gtgcgtgaaa acctgcgcct ccgccttttg gatgcgcgca ctgcattaat
    11461 gcagcgcaac agcgaagtct atcagggcga tttgaacaat gccgaagccg ccgtcagaca
    11521 gtatttcgat gccaagtctc ccgccacgca gtcgtggctg aaagaactgg cggaattgaa
    11581 ggcgttggat gtgcggatga ctgcggatga cggtttgaaa aacagcctaa atgccgtccg
    11641 cgcctatcgc gacggtacgc gcatgacggc ggcggaaaat caagaagcgg aacaggcggc
    11701 ttccgaaccg gcaaacgaaa aaacagcttc cgaaccggct gccgcatcgg atgtgaagac
    11761 catagaagca ccgtccctgc cttcggaacg caaaccggaa cagcctgcaa aaaaacagac
    11821 cgtaccggaa aaggcagggc gttcgccgtc cgctaaagga gaacgcgcat gaaaacggta
    11881 gtctggattg tcgtcctgtt tgccgccgcc gtcggactgg cgctggcttc gggcatttac
    11941 accggcgacg tgtatatcgt actcggacag accatgctca gaatcaacct gcacgccttt
    12001 gtgttaggtt cgctgattgc cgtcgtggtg tggtatttct tgtttaaatt cattatcggc
    12061 gtactcaata tccccgaaaa gatgcagcgt ttcggttcgg cgcgtaaagg ccgcaaggcc
    12121 gcgcttgcct tgaacaaggc gggtttggcg tattttgaag ggcgttttga aaaggcggaa
    12181 ctagaagcct cacgcgtgtt ggtcaacaaa gaggccggag acaaccggac tttggcattg
    12241 atgctgggcg cgcacgccgc cggacagatg gaaaacatcg agctgcgcga ccgttatctt
    12301 gcggaaatcg ccaaactgcc ggaaaaacag cagctttccc gttatctttt gttggcggaa
    12361 tcggcgttga accggcgcga ttacgaagcg gcggaagcca atcttcatgc ggcggcgaag
    12421 atgaatgcca accttacgcg cctcgtgcgt ctgcaacttc gttacgcttt cgacaggggc
    12481 gacgcgttgc aggttctggc aaaaaccgaa aaactttcca aggcgggcgc gttgggcaaa
    12541 tcggaaatgg aacggtatca aaattgggca taccgccgcc agctggcgga tgctgccgat
    12601 gccgccgctt tgaaaacctg cctgaagcgg attcccgaca gcctcaaaaa cggggaattg
    12661 agcgtatcgg ttgcggaaaa gtacgaacgt ttgggactgt atgccgatgc ggtcaaatgg
    12721 gtcaaacagc attatccgca caaccgccgc cccgagcttt tggaagcctt tgtcgaaagc
    12781 gtgcgctttt tgggcgagcg cgaacagcag aaagccatcg attttgccga tgcttggctg
    12841 aaagaacagc ccgataacgc gcttctgctg atgtatctcg gtcggctcgc ctacggccgc
    12901 aaactttggg gcaaggcaaa aggctacctt gaagcgagca ttgcattaaa gccgagtatt
    12961 tccgcgcgtt tggttctagc aaaggttttc gacgaaatcg gagaaccgca gaaggcggag
    13021 gcgcagcgca acttggtttt ggaagccgtc tccgatgacg aacgtcacgc agcgttagag
    13081 cagcatagct gattttggga aatatcttta tctgggagaa tttgatgggg tcttcagatt
    13141 cctttaagga aaagaaagaa atatttgaaa ttggaacgcc tgcttatcgc caaaagttaa
    13201 ttgatgtttg gaaaaagagc attaatggaa acgaaaaatc ttgggtgctc tttgaaaatg
    13261 ggacttgcgt cattttactt gaaccggaaa aagatttggc gaaacaagct aaagagatgt
    13321 taagcaaatg gggcaaggtt caaataggaa caccatctgc agattttggc attatcactt
    13381 tagatagtgg cgatggatat gccgtttcat gccatcatcc cgaaattttt acgctaatcc
    13441 taaaagaaga aggattggat gaagatttca aaatcggtat cgaagggcgc tctcatcgcg
    13501 attgtgatgc tgaagaaccc aaagttatcc atatcgaaga taaacgcacc attgaaaccc
    13561 catgaaaacc tgctgccgtt taatcatcta ctgatgatta cttaggcaaa tgtgcccgtc
    13621 cctccctttt cagacgacct ttcattgcgg aaaccgccgc aaaggttgtc tgaaaaccgt
    13681 tttccttccc cgttttacaa acaaaccgaa agccccacat gatctctttg aaaaacgaca
    13741 ctttcctccg cgccctgctc aaacaacctg tcgaatacac gccgatttgg atgatgcgcc
    13801 aggcggggcg ttatctgccc gaatacaaag ccacacgcgc gaaagcgggc agcttcctcg
    13861 atttgtgcaa aaacaccgaa ttggcgaccg aagttaccat ccaacctttg gaacgtttcg
    13921 atttggacgc ggcgattttg ttttccgaca tcctgaccgt ccctgacgca atgggcttgg
    13981 gactgtattt tgccgaaggc gaaggcccga aattcaaacg cgccctgcaa cacgaggccg
    14041 acatcgccaa gctgcacgtt cccgatatgg aaaaactgca atacgttttc gacgcggtaa
    14101 cttccatccg taaagcattg gacggccgcg taccgctcat cggcttctcc ggcagtccgt
    14161 tcacgctcgc ctgttatatg gtcgaaggcg gcggcagcaa agaattccgc accatcaaaa
    14221 ccatgatgta ctcgcgcccc gatttgctgc acaaaatcct cgataccaac gcccaagccg
    14281 ttaccgccta cctcaacgcc caaatcgacg cgggcgcgca ggcggtgcag attttcgaca
    14341 cttggggcgg cgtgttgagc gatgcggcgt ttaaagaatt cagcctcaaa tacatccgcc
    14401 agatcgtcgc cggactcaaa cgcgaaagcg aaggccgccg cgtgcctgtt atcgtatttg
    14461 ccaaaggcgg cgggctgtgg ctggaaagta tggcccaaat cggcgcagac gcattgggct
    14521 tggactggac gtgtaacatc ggcgaagcac gccgccgcgt cggcaagcaa gtcgccctgc
    14581 aaggcaactt cgacccgttc gccctcttcg gtacgccgga atccatccgc accgaggtcg
    14641 cacgtatcct agccgactac ggacacggca gcggccatgt cttcaacctc ggacacggca
    14701 tcaaccaaca cgccgacccc gaacacgcca aaatcttagt cgataccgta cacgagctgt
    14761 ctcggcagta tcacggcggg taagccggca ggaaaccgcc cgatatgccg tctgaagccg
    14821 agagatggcc ggttagggta aaaataaggc aatgcggcaa tatccgccgt gtacggatag
    14881 tacatgacgg cggcgttgtc gtattggcgc aatcccaacc gtccctatgt tcagacggca
    14941 tttttgtttt cagatgcagg gaaaaccgat ggcaaaaacg cttaaaaccc tttaccaatg
    15001 caccgaatgc ggcggcactt cgccgaaatg gcagggcaaa tgcccgcatt gcggcgagtg
    15061 gaacacgctt caggaaagcc ttgccgcgcc cgagccgaaa aacgcccgtt tccaatcttg
    15121 ggcggcggat acctcgaccg tccaatccct ctccgccgtt actgcgaccg aagtgccgcg
    15181 caatccgacc ggtatgggcg aactcgaccg cgtattgggc ggcggtttgg tcgacggtgc
    15241 ggtcatcctg ctcggcggcg atcccggcat cggcaaatcc acgctgctgt tgcaaaccat
    15301 cgccaaaatg gcgcaaagcc gcaaagtgct gtacgtttcc ggcgaggaat ccgcccaaca
    15361 ggtcgccctg cgtgcgcaac gtttggaact gcccacagaa ggtgtgaacc tgcttgccga
    15421 aatccgtatg gaggcgattc agacggcgtt gaaacagcat cagcccgaag ttgtcgtcat
    15481 cgactctatc caaaccatgt attccgacca aatcacgtcc gcccctggct ccgtgtcgca
    15541 ggtgcgcgag tgtgccgccc aactgacgcg catggcgaaa cagatgggca tcgccatgat
    15601 actggtcgga cacgtgacca aagacggcgc gattgccggc ccgcgcgtgc tggagcatat
    15661 ggttgatacc gtgctgtatt tcgagggcga ccaacattcc aactaccgca tgatacgcgc
    15721 catcaaaaac cgcttcggcg cggcaaacga actgggcgtg ttcgcgatga cggaaaacgg
    15781 tttgaaaggt gtgtccaacc cgtccgccat cttccttgcc agctaccgcg acgacacgcc
    15841 cggttcgtgc gttttggtta cgcaggaagg cagccgaccg cttttggttg aaattcaggc
    15901 attggtcgat gacgcgcacg gattcacccc caaacgactc accgtcggtc tcgaacaaaa
    15961 ccgcctcgcc atgttgctcg ccgtgttaaa ccgccacggc ggcatcgcct gcttcgatca
    16021 ggatgtgttt ttaaacgccg tcggcggtgt gaaaatcggc gaaccggcgg cggatttggc
    16081 ggtcatcctc gcgatgcttt ccagcttccg caaccgcccg ctgcccgaaa aaatggttgc
    16141 atttggagaa atcggtttga gcggtgaagt ccgccccgtc gcacgcgggc aagagcggct
    16201 caaagaagcg gaaaaactcg gcttcaaacg cgccatcgtt cccaaagcca atatgccgcg
    16261 caacgccaaa gagtttccga acctgaaaat ctacggcgtt tcgagtttgc aggaagccat
    16321 cgatatttgc cgtgacagca gggaataaac ggaaatgccg tctgaaatca ggtttcagac
    16381 ggcatttggt ttgtggcgga ttgaaacaag aaggcatacc ggcgacagat aagatttgcg
    16441 gcaaagttgc ctgtgatatg gcaaaacgca cacgcccgtc atccccgcaa gggcgggaat
    16501 ccggaatcgt ccgtttcggc aatgattgaa aatcacggta acccaaccgc ttggattccc
    16561 gacttcgtgg gaatgagggg cgtgtgtgtt tgatttccat ccgccatatg tcggcgacgg
    16621 gcttattcgc ctacggtttt ttgtatcagt ttttcggcgt ttgccaaagt gtttgccact
    16681 tcgtcaaaac cgatgcggct gccggcgatg agggcgcgcg tatcggtata ggcggcgcgt
    16741 actttgccgt ctgtttcggt aacgaggacg cgcaggggca gttgcagggc gaaggcgggg
    16801 tctttgacca tcagcggcgt gccggctttg ggcgtgccga agacgatgac ttttgccggc
    16861 tgcatcgtta agccgtttcg gtgggcggct tcctgatggt cgatgacggc aaaaatgtcc
    16921 atccctttgc tttttatggc ggtttcaagg cggctgacgg tttcgtcaaa actgtatttt
    16981 gaggtgaggg tatgcgtggt catagcggtt tcgttttggg tttgcggttc gctggcagga
    17041 tgtgccgaag cggttgaaat gcagagtgcg gatgcggcaa tcagggggag tatgtgtttc
    17101 atcgtatttc ctttttcctt ttcggtttgt ttatcaggct tcggacgggg cggcacgcgc
    17161 cgcgcccgat gttgcgccgc gtgccggggt gtcgtatgcc gtctgaaagc ctgtcctttc
    17221 agacggcatt gcgtcatttc atcccttttt tgagcaggtc ttcataaccg ccgtgattgg
    17281 caacattcgt atagcctgct tttttcagtt cttgaagggc ggcttcggca cgccgtccgc
    17341 tgcggcagta gaggttgacc ggcgtgtctt tgtcgggcgc ggcttcgtgt atgcggcgga
    17401 cgatttggtc gacggggatg ttgaccgcgt tgtgcaaatg accttcgcta aattcctgtt
    17461 cgctgcggac atcgatccaa acggctgaat gttgcgcggt ttgggcggcg gatacgggtt
    17521 tttgcggggc tgcctgcgcg gcaaaggcgg ctgaggcaat gagtgcggcg gtaatcaggt
    17581 gtttgatatt catagggttc tcctgcggtt gtcgtcttaa gggacgggaa gttattttat
    17641 ctgttccaaa gcggcggcat ctatgtccca atgccaaacg ccgccgcctt tgccgtctat
    17701 gccgcgtaaa aacaggtagc ggacggaaac ggcggcgggc ggttgtccgc gcagtttgaa
    17761 gtagcgcgcg gaggcaacgg cgtaaatcag tgcctgaagg taatagtgat ggtgcgcgac
    17821 ggcttcgtcc atcgcctgcc gcgtgtaggc ggatgcgtcc gcgccgagat ggtttgattt
    17881 gtagtcgatg acgcagatat ttccctcggg gtcttggcag accatatcga taaagccgtt
    17941 taaaaagccg ttgacggtgt ggaagtcgag cgtttcggcg gcggcacggc agacttcggg
    18001 cagcctgatg tcgtcgcggg caaaccagtc gcgcagccgt ttgaggccga agtcttcggt
    18061 gtggagggta aagcccattt cgggacagcg gcactcgggc gggatgtcgg acaggcagta
    18121 tgcccccgtc agcggcgttt tctggcaggc ttccgccatt tcggcaacgg cgggcagcca
    18181 catttcttca aacccgtatt tcttcagctt gtcggcaatg agagtttcct gtccggcggc
    18241 tgcttgtccg aatttgaaat cttcaagaat ttcgtgcagg cacagccccg cctgcgtgcc
    18301 tttcggaaaa tcgtgtatcg atatgccgtc tgaagccgtc ggcgtttcag acggcatcgc
    18361 cgctaccgag gtttcggcgg catccaagga cgggcaggca tcttcttcgc cgccgtcggg
    18421 cgtttgggta tggcggctta aggcggtaaa gctggtgtgg cggacaaatc ggaatctgcg
    18481 ttcgggaatg ctgtttgcgg caaattcggc ggtttgaccg gcgttgctgc gataggcggc
    18541 agggggcggc gcattttcgg tgaaggcgaa atccgtgccg gccggggcgt tgtccgccac
    18601 gcgccgccag ttttgtttga gcatcgttat gccgtccttt tcttgggcat aggtttggcg
    18661 gacggtttcg cggctgtctt gcggcaagcc ttcgattaga taggcgaggg ggttgtcggc
    18721 ggtatcggaa gaataggcgg cgtagatgtt gagctgttcc tcggcacgcg tcagcgcgac
    18781 ataaagcagg cgcaggcgtt ccgccatttc ttcatcggcg tattgtttct gttcgtcttc
    18841 ggacagttgc gcctttgcca acagttcggt tcggtttgcg ctttggtgga ggatttgcca
    18901 gtcggacggc ccggtatctt gcgcgtccca cgcaaacggg cagtacacca gcggatactg
    18961 taaacctttc gaggcgtgca tggtaacgat tttgaccaaa tcttcgtcgc tttccagacg
    19021 gatggcgcgg ttgtcgccgc cgttgttgcc ggccaggctg atttggtcgc gcagccattt
    19081 gtgcagcgcg gcggggttgc ggttttgcgc gtcttcggcg gcaagcagtt cgagcagttg
    19141 gaaatagttg gtcaggctgc gcccgttgtt ccggcttaag aggcgtgttt cgatgccgtg
    19201 tgtttgggaa aattgctgca tagcggcgaa aatgccgtat tgattccagt tgtcgagtgc
    19261 ggttcgggca gattccgccc aatgcaaaat ctcgctttcg ttttggttga agtcgtgcaa
    19321 ttgctgcgcg tcataaccga atatgccgct tgtcaggaca aagcgcagcg ttccggcgcg
    19381 gcgcggttcg agccagaagc cgatcagtgc ggaaagggcg gcggcttcgg gcgaggcgaa
    19441 cacagattcg cgcgaaagca ggacgctttg cacctgccgt tttttcaggg cagcggaaac
    19501 catcaccgcc tcgttgtgcg tgcgtaccag cacggcaata tcgcccgact gcaacgggca
    19561 gcctttgaaa ttcagacggc ctccggcggc ttcgttgagc gcgtgggcga tttcgtcggc
    19621 gcaatagtcg gcggcacggc ggcgcaaaac gtctttgttg gctttttcat tgtcgttttc
    19681 gtgcagccaa cgaacctgta cggcaggacg ttcgggggac aacctgcttt cggcacgcgc
    19741 cgcaccgact tccgaatagc cgatgttttc caaaacgaac gggcgttctt tgaggcggaa
    19801 cagcgcgcct atgctgccga taagcgcggc gtggctgcgg tagttggtgg cgagcgtgta
    19861 gcggtgccgc gcatcttccg ccgcctgaag gtaggcgtaa atgtccgcac cgcgaaagct
    19921 gtaaatcgcc tgtttgggat cgccgaccag aaacagcggg cggttttggg cgatgaagat
    19981 tttttggaaa atttcgtatt gcagcgggtc ggtgtcttgg aactcgtcga ttagcgcggt
    20041 ttcccagttt tcggcaacgg cgcgggcgag ggtttcggcg tgcggattgt cggtcagcgc
    20101 ggtgtggaca tcgagcagca ggtcgtcgaa accgcgttcg cggcgcgatt ttttcatctc
    20161 ggcaaggctg cggttgaggt atccgattaa atccagttgc agccggatta ttgttgcttc
    20221 ttccgcttct tcgagtgcgt tcaaatcgta cccgaagtct gccagtttct gcaattcggc
    20281 aaatactgcc gcatcgggcg ttttgccttt tttcagtccg gcttcgagtt tgtcggacgc
    20341 aagtttcaag agtctgtcgt gcgtgtcttt gtccagaaag ggcagttgtc cggcggcgga
    20401 tttttgtgcc agttctttaa aaaggttgcc gaagctgttt ttgcggtaac tgttgccgtt
    20461 gaggtcggga tggatgcgcc aaaagccggc ttccagctcg ggcagcaggc ggcagacggt
    20521 ttgccatgag gtttcggcgt tgcgctgcgc ctgtttcaaa tccgcctgcg gacggcggaa
    20581 attcaggtac gggcgggaca ggtaggcgcg gatttgggca aggacggttt gcggcacagc
    20641 tttgcgttta aacgccaatg cggcaagcac cggatcattg ctgacgcgtt cccgccaaaa
    20701 atcttgcgcc gggacaagca ggcggtcgcc gtcttcttcg gtcagttcga catcgaacgg
    20761 ggcttggcac aggaaggcgt agtcgcgcag gatgcgctga cagaagccgt ggatggtata
    20821 gatggcggcg ttgtcgaatt gcccgatggc ggccttgagg cggacaatca gacgcgtccg
    20881 gccctctttt tgcaaagcct gttttaagag tgtgggcagg aaggtgtcgc ctttgtggtg
    20941 ttcggcgcag taggcggcga tgccgtctga aagcgcgccg tctccaagtt cggcaatttc
    21001 tttgctttct aaaacctgca acacatcgtc caaacgcgcg cgcaggcgtg ttttcaactc
    21061 ggcggtggcg gctttggtga aggtaacgac caatacgcgc tcgacgtttt tttgttctaa
    21121 tacgatcagg cgcgtaaaca gggcggcgat gccgtaggtt ttgccggtgc cggcagaggc
    21181 ctcaatcagg ttggtgccgg caatggggac ggttagcggg tcgaatgctt ggatgcttgc
    21241 agacatagtg cgtgctcgga aaacggttgg acg
//
LOCUS       DAXHNE010000019        20694 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.019, whole genome shotgun sequence.
ACCESSION   DAXHNE010000019 DAXHNE010000000
VERSION     DAXHNE010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20694
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.019"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(61..237)
                     /locus_tag="ACFM4Z_000548"
     CDS             complement(61..237)
                     /locus_tag="ACFM4Z_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020636.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIGKVSAR"
     gene            complement(240..1598)
                     /gene="murF"
                     /locus_tag="ACFM4Z_000549"
     CDS             complement(240..1598)
                     /gene="murF"
                     /locus_tag="ACFM4Z_000549"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFC6020637.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLTALQMLAKAWRENVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGNECAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAVDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(1723..2859)
                     /locus_tag="ACFM4Z_000550"
     CDS             complement(1723..2859)
                     /locus_tag="ACFM4Z_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFC6020638.1"
                     /translation="MDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILE
                     AAKKASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQ
                     STAHATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRS
                     DYPWFTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDK
                     DARRFQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGE
                     RTLTHGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDG
                     YDFWGNVPDVQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
     gene            complement(2933..3370)
                     /locus_tag="ACFM4Z_000551"
     CDS             complement(2933..3370)
                     /locus_tag="ACFM4Z_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020639.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWRYCVSGGGIC"
     gene            complement(3393..4871)
                     /locus_tag="ACFM4Z_000552"
     CDS             complement(3393..4871)
                     /locus_tag="ACFM4Z_000552"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFC6020640.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKVWGVAGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDEYGAELVGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDNVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(4896..6641)
                     /locus_tag="ACFM4Z_000553"
     CDS             complement(4896..6641)
                     /locus_tag="ACFM4Z_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFC6020641.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(6702..6965)
                     /gene="ftsL"
                     /locus_tag="ACFM4Z_000554"
     CDS             complement(6702..6965)
                     /gene="ftsL"
                     /locus_tag="ACFM4Z_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFC6020642.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            complement(6962..7948)
                     /gene="rsmH"
                     /locus_tag="ACFM4Z_000555"
     CDS             complement(6962..7948)
                     /gene="rsmH"
                     /locus_tag="ACFM4Z_000555"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFC6020643.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRRIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PELPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            complement(7945..8400)
                     /gene="mraZ"
                     /locus_tag="ACFM4Z_000556"
     CDS             complement(7945..8400)
                     /gene="mraZ"
                     /locus_tag="ACFM4Z_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFC6020644.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVSAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            8679..9896
                     /locus_tag="ACFM4Z_000557"
     CDS             8679..9896
                     /locus_tag="ACFM4Z_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6020645.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQFSLKLQTLI
                     AEKIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLEHGCMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHIIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYL
                     PQSHFLLNLGITELLAQTGKTDSAAYICEAAAVQKLIDQHEMGELFKVIAFGKNIGID
                     WVGFRFGDICHKL"
     gene            10028..10104
                     /locus_tag="ACFM4Z_000558"
     tRNA            10028..10104
                     /locus_tag="ACFM4Z_000558"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10062..10064,aa:Met,seq:cat)
     gene            10112..10187
                     /locus_tag="ACFM4Z_000559"
     tRNA            10112..10187
                     /locus_tag="ACFM4Z_000559"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10145..10147,aa:Ala,seq:ggc)
     gene            complement(10478..11299)
                     /locus_tag="ACFM4Z_000560"
     CDS             complement(10478..11299)
                     /locus_tag="ACFM4Z_000560"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFC6020646.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEHLFNPLSVAVMLVLGGLFILWVEKRQSWAEPKIADVDALRPIDALMIGVAQVFA
                     LIPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFVAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(11332..11976)
                     /locus_tag="ACFM4Z_000561"
     CDS             complement(11332..11976)
                     /locus_tag="ACFM4Z_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC6020647.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRSVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYGIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(11979..12824)
                     /gene="ftsN"
                     /locus_tag="ACFM4Z_000562"
     CDS             complement(11979..12824)
                     /gene="ftsN"
                     /locus_tag="ACFM4Z_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC6020648.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPA
                     SSKQPAETEILKPKNQPKEDIQPEPADQNALYEPDAATEAEQSDAEKAADKADEVEEK
                     AGEPEREEPDGQAVRKKVLTEEREQTVGEKAQKKDAETVKKQAVKPSKETEKKASKEE
                     KKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTSDKAEATHYLQMGAYADRR
                     SAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHEVASLIR
                     SIESK"
     gene            complement(12930..14426)
                     /locus_tag="ACFM4Z_000563"
     CDS             complement(12930..14426)
                     /locus_tag="ACFM4Z_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFC6020649.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLE
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECQVPQMPFEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDTSARIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(14440..14760)
                     /locus_tag="ACFM4Z_000564"
     CDS             complement(14440..14760)
                     /locus_tag="ACFM4Z_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HFC6020650.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            15243..16769
                     /gene="putP"
                     /locus_tag="ACFM4Z_000565"
     CDS             15243..16769
                     /gene="putP"
                     /locus_tag="ACFM4Z_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFC6020651.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSTAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRATR
                     "
     gene            complement(16859..17026)
                     /locus_tag="ACFM4Z_000566"
     CDS             complement(16859..17026)
                     /locus_tag="ACFM4Z_000566"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020652.1"
                     /translation="MKHFLDSPKFFGGQAASVRPEYGNIVINPQMKYKASNAGRVGAS
                     VSLKMKRVKHK"
     gene            17018..20623
                     /gene="putA"
                     /locus_tag="ACFM4Z_000567"
     CDS             17018..20623
                     /gene="putA"
                     /locus_tag="ACFM4Z_000567"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HFC6020653.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRASRTKASGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYAGEAENTLPKDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVAHLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTELAASGIQAVVEPDSPLASYTADLEGLLLVNGKPETAGISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         4862 a         5988 c         5026 g         4818 t
ORIGIN      
        1 ggcctgcgcc cgtttgaggt tggcttgccg cccggcaagg tttcggacga cttttgcaga
       61 ttaacgtgca gagactttcc caatcacgca aatcgggtaa ctccccggat ttttgccgcc
      121 acccaagtct aaacataaat cttcataaag atacgcctgc gtctgcattc cggcataaaa
      181 cgcgccgacc agcgcggcaa tcaacaataa ggctttaaaa aaacgtcgac ttttcatttt
      241 cacttatcct ccaatgcctc gaccacttct tccatctgca taaagcgcga acctttcacc
      301 aacacggtgg cgcgttcggg caaatcgtgg cgcaacactt gaatcaacgg gtctttggcg
      361 gcgaaccaca aaccgtccgc gccaaatttt tccgccgctt cgacgctgtt gtcgccgaca
      421 aaataagccg cttcgatgcc ttggtcgcgg gcgtacgcgc cgacttcggc gtgcatggcg
      481 gcggcttcgt cctcacccag ttcgcccata tcgcccatca cgaaaatacg cggcgcaggc
      541 atacgcgcca acacgtcaac cgcagctttc atgctgtcag ggttcgcatt ataagtatcg
      601 tcaatcaggg ttgcgccctt gattccggat ttgacgttca gacggccttt gatattgctg
      661 aagcctttca aaccttccgc cacatcgttc aaactcaaac ctgcagccaa agacagcgcg
      721 gcggcggcgg cggcgttgtg gacattgtgg cggccgggaa cgggcagaac cacggctgcg
      781 cactcgttgc cgcacaccaa atcaaattcg cacgacaacg gtttcagcac gatattttcc
      841 gcgtggacat cgccgctatc gatgccgaaa gtgcgcgtat tcaaattaag cgttgccgtt
      901 ttgaagacag ccatattggc atcttcttga ggaatcagcg ccatgccgtc tgaacataag
      961 ccttgataaa tctcgctttt cgctttggca atatcgccca ctccgtcgaa accgcagccg
     1021 acatgggcgc gcatggcgtt gttgaccaat gcggcatcgg gtttggcgat ttgtgtcaaa
     1081 accgccagtt cgccaaaatg gttcataccc atttcaatca cggcatagcg gtgtttttcg
     1141 tttaatttca acaaagtcaa cggcaatccg atgtggttgt tgaagttgcc tgccgtcgcc
     1201 aaaacggcat tatcgccgaa acggcggcgc aataccgcag ccaacatttc cttcaccgtc
     1261 gtcttgccgc ccgagccggt aataccgaac acaaacgggt tcacattctc gcgccacgcc
     1321 ttcgccaaca tttgcaacgc ggtaagcgtg tcatcgactt tcaacgcgcc atccattgca
     1381 acgcaatctt cgcgcgaaac cacaaccgcc gccgcacccg cagccaatac gtcttcaaca
     1441 aaatcatgcg catcaaaccg cccgcccgcc aatgcgaaaa acacatcgcc cgcgcggatg
     1501 tcgcggctgt cggttacgat gcgcgacacg ggtttgcttt cagacggcat cggaagcttg
     1561 agggcttggc agatgaaatt taggtccagt ggtttcatat ttactttcgt taatattcgg
     1621 gcggcggaca catcggtagc ggctgatttt tttatcgcct gttttgctgt ggtaaaacac
     1681 agattatttt cccattctca ttcggcattt tttctgtacg tatcattttt tagacgtatt
     1741 tttagtcgat ttgccttttc ccgcatacca cggcgcgggg tcgtcgggca gtccgtcgat
     1801 aaaggcaagg ttattgcctt cgccctgcac atcgggaaca ttcccccaaa aatcatagcc
     1861 gtcatcgggc aactcgtcgg tttcgatacc cgtccaactg ccgaatccgc gtaaaaaatt
     1921 aaacgcgctg cgcctcactt taatcatttc gcgccgcgta tcatcggacg aaattttaac
     1981 cagcggcacg ccgtagcttt gacgcttcca cgcaccatgt gtcaacgttc gctcgccgcc
     2041 tttaccgaca tgcatcaaac cgtggtcgga aaagtaaacc aaagaccagc tttctttatt
     2101 ttcattcaat atcttaactg tatcttctaa aaatttatcg gtttgcgcga tggtggaaac
     2161 atagcaggat attttttcag tttgatactg aaaccgccgc gcatccttat ccaaacgtgt
     2221 gcaaaaatca ctgtgcgaac ccatcaggtg catcacaatc agccgaggct tcgtgcctgc
     2281 atttcctatc aaaacccgtt tgaacgccgg caacaaaagg cggtcgctca accccgcgct
     2341 tttgccataa tcaccccttt gggtaaacca cggataatcg ctgcgtaggg cataggtgga
     2401 aatttcgttg gcaaaatgcc ccaacattcc ttgattagac agccacgccg tccgaaaacc
     2461 cgcctgcttc gccaagctga cgatgttatt gttcggttct cccggcagcc ccaaagtctg
     2521 cggcagcgaa agattcgtcg cgtgggcggt cgattggtaa ccgtttatca acagcccttt
     2581 ggtctgactc aaaaaaggcg tatcgggcaa tgggaaaccg taaacattca tataatccga
     2641 acgcgcgctt tcaccgataa ccacaacata atttttatac ttgggcgcaa catgacggat
     2701 atgccatgtt gacgcttttt ttgctgcttc caaaatgtgg gcgcgcttgg cggcatattg
     2761 tgccggcgcg gaagccaaat cgtaatacag gtcgaaaata ttcaacaaca ggccggcatc
     2821 aggttctcgc aaatctttat cgctggcgat tttatccatc accgcgcagg acaaaatcaa
     2881 agtcaataat atagtcagcc atattttgct gcggcgttta tagtttttta cgtcagcaaa
     2941 tacccccccc cgatacacaa tatctccaaa ctgtcaagcc aaaaatgaaa aataatgcct
     3001 gcacaaaata aagcgacccg ggaagattgc cgacaaattc acgcgcctcg gcaggattgc
     3061 tttccaatat cgaaccgact atctgataag acggcgcacc atacagccag ccgaccggca
     3121 aatatagggc ggttgtgccg acgtaaatca gtaaaacaac tgaagaaacg cgcgggaaac
     3181 cgcgtgccaa taataataaa aatacaaccg aagccaaagc acccacggca gaataacggt
     3241 agccgtattc atattccaag tgataacctg atgcgatggc cgcgccaaaa caaaaggcta
     3301 aaagcgcgca aatgcccaat gtatttttta atgtctgatc catatttttt attcgggcgg
     3361 attgtttcat acgatgccgt ccgaatatcc cattatttac gagttaacaa agcctgtccg
     3421 acgatttcaa gatcggaaaa acggtgcttc acgccttgta catcctgata gttttcatgc
     3481 cctttgccgg caatcaggat gatgtcgttt gcggcggctt gttcaaccgc ataacggatg
     3541 gcggcggcac ggtcggcttc gacgcattcg ggcgcgggaa cggcaggcag gatgtcgttg
     3601 atgatgtcgt gcggattttc caaacgcgga ttgtcgctgg tgacgacgac tttatccgcg
     3661 ccctgtacgg ctgccgcgcc catcagcggg cgtttgccgc gatcgcggtt accgccgcaa
     3721 ccgaatacgc accacaaagc cgcaccctgc ggtttgattt cctgcaaggt ggcgagtgct
     3781 ttttccaatg cgtcgggcgt gtgggcataa tcgacaacga ccaaaggttt gccgctgttc
     3841 atgatgcagt ccatgcgccc tgaagcggga cggatttttg ccagcacatc caatacatta
     3901 tcaagcggat agccgttggc gcacagcaag gcgatgcagg cggcgaggtt ttgcgcgttg
     3961 aaccgtccga gcaggcgcgt gcggcatttc ccttcgcccc acggggtttg gaatacggct
     4021 gctatgccgt ctgaagaggc ggtaaagtcg gtaatgcgga tgtcggcgtg ttcgctgaaa
     4081 ccatagctgt aaacggccaa atcgggacag tcttttttca gacgacctac gagttccgcg
     4141 ccgtattcgt catccacgtt gatgactgcg tgtttcaagc cgtgccagta aaacaggcgc
     4201 gacttgatgg caccgtaggc ttccatcgtg ccgtggtagt cgaggtggtc gcgggtgagg
     4261 ttggtaaaga ttgcgctgcg gaatgacacg ccgttgacgc gcgactggtc aagcccgtgg
     4321 ctggagactt ccatcgcggc gactgttgcg ccttgttgac ggaaacggta gagcagggtt
     4381 tggacatcga cgggggcggg tgtggtatgc gtggtttctt ccaatgcacc ccaaaagccg
     4441 ttgccgaccg tgccgacaat ggcggttttt tcgcccaaca aatcggcagc ttgcgccagc
     4501 cattgtgtga tggaggtttt gccgttggtt ccggctacgc cccaaacttt gaggccgtct
     4561 gaaacgttgc cgtaaacttg cgccgccaat atgccggcac ggtgtttcaa atctttgatg
     4621 ccttgattgg ggactttcca ttcgggattc cacgcaaatt tgccgtcgtc gtcccaaaaa
     4681 acaaaagccg cgccgttggc aacggcggcg gggatataac tgcgtccgtc ggcatattcg
     4741 cccggacagg caacgaaaat atcgccttgt ttgatttggc ggctgtctga atgcaacaaa
     4801 cgccctgccg cgtttgcaca cgacagagtc gggatgccgg tttcagccaa aggggttaac
     4861 ttgctgaaca taaaacaatc tcgttgatac tcggattaag acggtgtttt gacggctgcg
     4921 gcggtcagtg gcttggtcgg ggaaatgccc aagatgttca ggctgccgcc cataattttt
     4981 ttgaagggcg gccctgccac tacgccgccg taataaccgt gggcagtcgg ttcgtcaatg
     5041 gttaccgcca caatcacacg gggatttttg gcgggggcaa aaccgataaa ggtagcgatg
     5101 tgtttgttgt cggcataacg cccgttgacg aacttgcgcg ccgtgccggt tttcgcgccg
     5161 acatcgaaac cgtccaccgc acccgccgta ccggtgccgc ccggctcggt tacggaaacc
     5221 atcagattgc gtacctcgcg cgcggtcgat tctttgaata tgcgtttgcc ttgcggcgca
     5281 accgcctgtt tttcaaagct gaccggcagt aaaacgccgt cgtgcgtcag tgcggtatag
     5341 gcgcgcgcca attgcagcag gctcaattgc aggccgtaac cgaaagacat cgtcgcctgt
     5401 tcgataggcc gccacctgcg ccaatttctc aacaaacctg cagtttcgcc cggaaagccc
     5461 gagtgcatac gcacaccgat gcccaactca tgatagaagt catacatttc ttcggcaccg
     5521 aaacgcgcag acagtttgct tgtgccgacg ttggacgatt tctgcatgat gccgcgcaca
     5581 tccaaagagg ggtaaacatg ggtatcgcgc acgggagacg gtccgatttt ataaggctgc
     5641 gtattcagcc gttcgttcaa atcggttttg cccgcatcca atgccttcgc aatcacaaac
     5701 ggtttgattg ccgaaccggg ttcgatcata tcggttacgg cacggttgcg ccgctgttcg
     5761 ctgtctgccc ggccgggcct gttgggatcg taggcgggcg tattggccaa ggcgaggatt
     5821 tcccccgtgc gggcatccaa aaccaccacc gttccggctt ttgcctgatg gtattcgacc
     5881 gccttgttca actcttcata ggccaaggtc tgaatcctct gatcgaggga aaggatgatg
     5941 tctttgccgt ttttcggggc tttattgcgc ggggagtcca agctgtccac aatattgccc
     6001 tgccggtccc gcaaaacgac ttccgcgccg tcttcgccat gcaggctgtc ttcaagcgaa
     6061 agttccaaac cttcctgacc tttgccgtca atatcggtaa atccgatgac gtgtgcaaac
     6121 aggttgccca tcgggtaatg gcgttttaat tctttttcaa atacaaagtt ttccaaaccc
     6181 aaggctttga cctcttcggc aaccttggga tcgagctgcc gcttaatcca gataaacgac
     6241 ttgcctttct gttcgagctt gttcctcaaa acatcaaccg gcacatcgac aagctcggac
     6301 aggcgttcca attgtgcggc agacggcatt tccttcatct ctttaggcac ggcaaacagg
     6361 gactccgtcg gcgcactcaa cgccaaaacc gcaccgttcc ggtccgaaac cgtaccgcgt
     6421 gtagccggca atgtttgagt ccgcacaatc cggttgtcgc cctgttcttt caaaaagtta
     6481 tacgttaccg tctgcagata cagtccgcga gcaatcagac cggcaaacaa gaccgctatt
     6541 gccatcagga cgaagctgat ccgtccgtta ctggtcatcg gctttttgac ctgctcttct
     6601 ttgggcagca tccgaggctt atattcgctc ttaatcaaca tttttacttc tcgttattat
     6661 tatcctgacg caggaatccg attccggcac acaggctgct tctatctttg atgctccacc
     6721 ataaaggtat tgcccgaaac cggcggatgg aggttttgtt tttctgccgc cgccctgatc
     6781 gcttcgtggt tcgccaaacg cgcctgttgc agcctcattt gcgcataatc ctgctccaag
     6841 gcgatttcct gttttttcgc cttatccaaa gctgtgaaat tgagcctgta ctggttttgc
     6901 tgcatcacaa cggaaaaagc ggaaacgcac accgcaagca gcagaaggaa attcaatttg
     6961 ttcattgcca ttcagacgtt tttctctgtg attgttccgg tatcggaccg gcagtccgct
     7021 ccgccacacg caaaaccgca cttctcgccc tcggattggc ggcaatttcc gcctcacccg
     7081 gctttaatgc cctgcccacg attttcaggg gcagctcggg caaatccgct tccctgaccg
     7141 ccgcccagcg cggcaggggc gcgtgttgcg aatatttttt gacaaactgc ttcacaatgc
     7201 gatcttccaa cgaatggaaa gcaatgaccg ccaaacgtcc gccctctttc agacgacaca
     7261 tgacctgcgg caatactgcc cctacttctt caagctcgcg gttaataaag atgcggaccg
     7321 cctggaaggt gcgcgtcgca ggatcctgcc cccgctcgcg agtacggacg ttttgtgcca
     7381 cgatctgcgc cagcttgcgg gttgtatcga ttggactttc cgcccgttgc gcaacaatgg
     7441 cgcgcgcaat ccggcggcta aaccgctctt caccataatt cttgattacc tcgtgcaaat
     7501 cctgttccga cgcaaccgct atccactctg cggcagacat accgcgcgtc gtatccatac
     7561 gcatatccaa aggggcatcg aaacggaagc tgaaaccgcg gctgccgtca tcgatttgcg
     7621 gggacgaaat ccccaaatca aacagcgcac cgtccacctt gccgataccc aaaccgtcca
     7681 atgccgtctg aaacgaagca aaaccgccat gcacgacacc gacccgtttg tccgaacgcg
     7741 ccagctcttc tgccacagca atcgcctgcg ggtctttgtc gaaaacaatc aaccgccccg
     7801 catcgcccaa acgcgacaaa atcagccggg aatgccctcc cctgccgaac gtaccgtcca
     7861 catagacacc gtcttcgcgc acggcaagcg catccaccgc ctcattcagc aagaccgtga
     7921 tatgccggta actttctgct ccactcacaa ttgcaaatcc gtctgactca actggaaggc
     7981 aagttcgtca ggatcgtcat ccaaagcctg aaccatctca gcctcccact gctcgcgacc
     8041 ccaaagctcc aaacggttgg cacgaccgac caaaacgact tcacggtcga aatccaccct
     8101 cttcctcagt ccggcagaaa ccagcacccg gccggcgctg tcccattcca aaatttccgc
     8161 gttatgcagc aaaagatttt gaaaccgccg caaaacaggg ttatccgcca cttttaagtt
     8221 taaaagttgc gccgcaacct tttcccactc cgcaacaggg tacatcaaca gcttgtgttt
     8281 cgactcgagc gttaccacta cggcaggcgt atagaggcgc gacagaatgt cacggaattt
     8341 ggcaggaaca gccaaccgcc ccttactgtc gatgcttaat tcgtgtgcgc cgccgaacat
     8401 gacatgtccc aagccgaaat caaaatcaca agggtaaaag agacactttg ccccacaatt
     8461 ccccaccaat cgacactata agaaatttta aacactcggt caaatcaggg catgaaaact
     8521 cattaacata tctgaaattt ttattccttt taaaaacaat aagataaaaa atgacgacaa
     8581 cggcacggcg ggtgcggtac agaataatcg aaccaataaa caactatata tgattaattt
     8641 aataatataa acacaatata tagtattaag ataaagccat gacagcaccc gtaccaacgt
     8701 gtaatatgtc gggaaatcca ataaatttac acaagctaac acttatcatg cccctccccc
     8761 tcccctctcc cgaagcacgg caattctcgc tcaaactgca aaccctcatt gccgaaaaaa
     8821 tcggcaaaca cggcaactgg attccatttt cacgttttat ggaattggtt ttatacgctc
     8881 cgcaatacgg ctactacacc ggcggcagcc ataaaatcgg caataccggg gattttatta
     8941 ccgcaccgac cctcacctct ctgtttgcac agacactggc acgccaactt caagaacttc
     9001 tatcccaaac ggcgggcaat atctatgaat tcggcgcggg aaccggacag cttgccgccg
     9061 atttgttggg cagcatttcg gacggcatca gccgttacta tattattgaa atatcgccgg
     9121 agctggcagc acgtcagaaa aacctgattc aagcacgcgc accggaagca tctcaaaaag
     9181 ttgtccactt gaccgcactt cccgaagcgt ttgacggcat catcatcggc aacgaagtac
     9241 tcgatgccat gcctgtcgaa atcgtccgta aaaatgaagg cggctcattc gagcatgtcg
     9301 gcgtttgcct agataatgac cgttttacct attcggcacg accgctgcac gacttgcagc
     9361 tatctgcctt ggcttccctc tattttcctc aaacagatta tccctatacc agcgaactac
     9421 atccgcaaca atatgccttt atccgcaccc ttgcctcaag actcgaacac ggctgcatga
     9481 tattcatcga ctacggtttt gatgcagcgc agtattacca ccctcaacgc aatcaaggta
     9541 ctctgatcgg acactaccga catcacatta tccacaatcc ttttgacttc atcggattgg
     9601 ccgacctgac cgcacatgtc aactttaccg acattgcaca agcagggacg gatgccggat
     9661 tagatttgat aggttacctt ccccaatccc atttcttatt gaacttgggc attaccgagc
     9721 tattggcaca gacggggaaa acggattcgg cagcctacat ctgtgaagct gctgccgttc
     9781 agaaactgat tgaccagcat gaaatgggcg aactgtttaa agtcatcgca ttcggaaaaa
     9841 atatcggcat cgactgggta ggattccgct tcggcgacat ctgccacaaa ctctaaccct
     9901 catgccgcct gaatccgctt cagacggcat aaacttttta acatttaaaa acagtcaact
     9961 aattcaaaat taaaaaatac ggcttgtcaa aaaaacagaa aaacatataa tagcgtcttc
    10021 acgaaacggc gaattagctc agtcggttag agcagaggaa tcataatcct tgtgtccggg
    10081 gttcgagtcc ctgattcgcc accaaatttt cgggggtata gctcagttgg tagagcgctt
    10141 gcatggcatg caagaggtca gcggttcgat cccgcttacc tccaccagat aaaaaagcac
    10201 agaccgtaaa aaggtatgtg cttttttatt acctgattac cggcaaataa agaataaacc
    10261 actgccttca aaacaggcaa tcgactttaa acctatcgcc ccgcctgtcc tgattttata
    10321 gtgaattaaa tttaaaccgg tacagcgttg gctcgccttg ccgtactatc tgtactgtct
    10381 gcggcttcgt cgccttgtcc tgatttttgt taattcacta tatcagcccg ccagacaaac
    10441 ccgacccgaa taatgtcttc aggtcgggtt tatggtttca ttcccaactt atccagcctg
    10501 acagccacaa tataatgatg gcaataccaa aaacaatgcg gtaataggca aaaggaatat
    10561 aatttttctt ggaaacaaac ctcagcaacg ctttgaccgc caccaagcct gaaacaaagg
    10621 cagcaacaaa gccaatcaaa atcaaaccga catcatgcag ggtgaaaaac cggtaatgct
    10681 tcaggacatc ataagccgtt gcggcaacca tcatcggcac agccaagaaa aacgagaatt
    10741 ctgtcgcagt tttccgttcg attccccaaa gcatcccgcc cataatcgta ctgcccgaac
    10801 gggacgtacc cggaatcagt gcaaacactt gggcaacgcc gatcatcaag gcatcaatcg
    10861 gacgcaatgc atcaacatcg gcaattttag gctctgccca gctttggcgt ttctccaccc
    10921 acaaaataaa caaaccgccc aaaaccagca tgaccgccac actcaaagga ttgaacagat
    10981 gttctttaat ctgtttgccg aacaacagcc ccatcacggc ggcaggtata aaagcaatgg
    11041 caagattaag gacgaaacgg ttggctttcc ggtcttttcc cacgccatgc aacacattgc
    11101 tgaaacgttg ccggtattca aacactaccg ccaaaactgc accgagctgg atggcaattt
    11161 caaaaacctt gtgattgctg tgaaaaccaa tcagattgcc gaacacaatc aaatgcccgg
    11221 tgctggaaat cggtaaaaat tcggtaaaac cttctaccaa gcccatcatc agggctttca
    11281 ggacaatcag aaaatccatt gcttgcgctt ctttcggata cgggagttcg gctatttctg
    11341 tacagcaggg gtctgacgct tgcgttcttc cctgactttg gcaaccaatt ctttaatcgt
    11401 atgaacgccg ccgtcaaagc cgttattgaa gataacgcgg tattttccgc cgacaataac
    11461 ggtcggcgtg ctgtcaatac cgtattgttc cgtcagtttc tgcattttta atgcggcggc
    11521 ggcagcttcg ggagaatcat aggcgcgcat cagttttttg ccgtcaaagc ctttttgaga
    11581 caaagcccac tttccggcaa ccgacctgtt ttccaagcgg attttttgtt cgtaaactgc
    11641 tttaaacaca gcagggtttg cctgatattt caaacccgac aaattgacgg cggccgccat
    11701 cctggccaga ccgagcattt caggctgcca gaccacgtgc tccgtcctca aataggcatc
    11761 agacggcaat gccttgccca gtttcaataa caaaggatcg aaatgatggc aatgtacgca
    11821 gaaatagccg aaaaattcca aaacctcaat tttacccgac tgttcttgag gaatgggttt
    11881 atccaacaca agatagtctt ccccttccgt cagggcatat gcctgcgcgg acaacactgc
    11941 cgacagcagc agcggcaaca gatgtttgag cttcataatt atttgctttc gatagaacgg
    12001 atcaggctgg cgacttcatg ttttttcaac tcgtcctgca tttttttcac cgcatcggca
    12061 gacatattgc cgctttgcac ccggtaaagc gttttatgtc ccgcctgata accgaccacc
    12121 ttggaagata tgcccaagat tgccagtttg gcacgctgcc cttccgcgct ccggcggtcg
    12181 gcatacgcgc ccatttgcag ataatgcgtt gcttccgcct tgtcggacgt tttcattttc
    12241 tgcacttctt tggcggcggc actgcgcgct ttttcgatgc tgccgctgtt gaggatttgt
    12301 tccggggtcg gtttgggtgc aactttttcc ttttccgcct ttttctcttc ttttgaagct
    12361 tttttctctg tttctttaga cggttttacc gcttgttttt taaccgtttc ggcatctttc
    12421 ttctgcgctt tttccccgac ggtttgttca cgctcttccg tcagcacttt cttacgcact
    12481 gcctgtccgt ccggctcttc ccgttccggc tcgcccgcct tttcttcaac ctcgtcggct
    12541 ttatcggcag ctttttccgc atccgactgc tctgcctctg tcgcagcatc cggttcgtac
    12601 aaggcgtttt gatcggccgg ttcaggttgg atgtcttcct taggctggtt tttcggtttc
    12661 aggatttccg tttctgcagg ctgcttcgac gaagccggga ttttgaacgc attttgaccg
    12721 ctctggttca gataaaacaa aataccggca ataatgaccg tcgccagtat caaaccgaag
    12781 aaaaaaccgg acagaccttt tccggattgg gaaaatttgt tcataaacat accttaatgt
    12841 gtttcagacg gcattaacgc cgtttgctta cgggcggata ttctaacaat atcgccatat
    12901 ttgggcaaaa cctgcttcca ttcccgttcc tataaagcgc gacggaaacc tatggctttc
    12961 atgacgtggc ttctgccgac ttcttcgtcg cccgccaaat ccgccaatgt acgcgccacg
    13021 cgcataatgc ggtggaagct gcgggcggaa agggagagtt tttccagcag gccgcccaat
    13081 gcttcctgcg cttctttttg aatgcgggcg gatgtgtcga gttcactgac actcaaggcg
    13141 gcattcactt tgccttgccg tgcgtattgt ttgtctctag cggcgataac gcgttccaaa
    13201 acggacgcgc tgctttcgcc cgcttcctgc tgcatcagtt cggcggcgga caggctcggg
    13261 acttcgatgg tcaaatcgat gcggtcgagc agcgggccgg aaatcttgct gcggtaacgc
    13321 gcgacgcttt cgggcgtgca gcggcagggc ttgacgggat gcccgagata accgcacgga
    13381 cacgggttca tggcggcgac aagttggaat ttcgccggat agacggcttg gcgcgccgcg
    13441 cgggaaatgt ggatttcgcc gttttccaaa ggttcgcgca acacttccaa aactttgcgg
    13501 tcgaactcgg gcaattcatc caaaaataaa acgccgtgat gcgccaatga aatttcgccc
    13561 ggacgcggat ccgaaccgcc gccgaccata gccgccgcgc tggcgctgtg atgcggactg
    13621 cggaaaggac ggttgctgtc gagttgttgc tggtggttgg gcaggagcga acgcaatgcc
    13681 caaacttcta ccaattcgtc ttcggtcagc ggcggcagga tgccgggcag ccgttgggag
    13741 agcatagact tgcccgttcc cggcggaccc atcatcaaga ggctgtgtcc gcctgcggca
    13801 gcgatttcca aagcaaggcg cgcggtgtgc tgacctttca catcgcacaa atcaggttgt
    13861 ccgccatgtt caaacggcat ctgaggaact tggcattcgg tttgcgccaa aggttcgatg
    13921 ccgttcaaat gggcggcgac ttcgcccaaa gagcgcgcgc cgtaaacggt aatgccgcgc
    13981 atcacggcgg cttgtcctgc attttcttca ggcaaaacaa atgcacgttt tgcctgcata
    14041 ccctgccacg ccatcgccaa cgcgccacgc acggggcgca acagccccga cagtgccaat
    14101 tcccccgcaa actcgtattc ctccagtttt tcgggcgcaa cctgccccga tgcggcaagg
    14161 atgccgattg caatcggcaa atcgaaacgc cccgactctt tgggcaggtc ggcgggggcg
    14221 aggttgacgg tgattttttt ggcggggaat tcaaaaccgc tttgaataat ggcggcacgg
    14281 acgcggtcgc gactttcttt gacttccgta tcgggcagcc cgacgatgtt gaaatgtggc
    14341 aggccgttgg caaggtgggc ttccacttcg accaacggcg cattcatacc gctcaaggcg
    14401 cggctgtaaa ccaaggcaag cgacatattt cagacggcct tattcgccgg cttcggtttg
    14461 ctgcctgatt tcggcgacgg cttcttcggc agcggcttca gccgcttcca atgctgcccg
    14521 ttcgggattt tgcgcggctt cgagttttgc caaacgcgct tccaaagccg ccagtttggt
    14581 acgagttttg attaaaacct gctgctggat gtcgaattct tcgcgcgtaa ccagatccat
    14641 acggttgaac gcgccgccca gcatcgcctt aatatttttt tccacatctt tggcagggct
    14701 gttggcgatg gtttcgctga ttttcgaacc gacttcttca aaaagctgct tgccgaacat
    14761 aatctgtatc cttcctgaac atatcaaatt caatcggcta ttgtataagg aaaaatgccg
    14821 tctgaaaacg ggcgacggat aatcggcaaa acataccgcg cctcctttgc ggttgctaaa
    14881 ttacacctca aaacacctgc cgcctgaaaa cggatttcag attccgcgca acgcctgatt
    14941 acaagacact acaaaacaat atgctgtttt aaatgatttt tccgacgcgc atcgtttcaa
    15001 actcggcttt ttaagccatt aagtgctttg caaacaacag aaattgggtt atgctgaaac
    15061 ggattattta caatttcata tagttttatt acatatctta ttgtgattga agataattta
    15121 tccgaatccc cctttcgggt atccggattt tccgttgtac ttttattaga aaaacatttc
    15181 aggcgcaagt tgcttgcaat ttcaaagcct gtaattctga agttttagac gaggaatagc
    15241 acatgaatcc catgtacatt acttttgcaa tctatctggt tgcagtcctt ctcatcgggc
    15301 ttgccgccta tttttctacg cgcaatttcg acgattatat tttgggcggg cgcagcctag
    15361 gcccgtttgt taccgcgatg tcggcaggcg cgtccgatat gtccggctgg ctgctgatgg
    15421 gtctgccggg cgcgatttat ttgagcggtt tgaatgaggc ttggattgcc atcggcctct
    15481 tggtcggcgc gtatttcaac tggcttttgg tggcgggccg tctgcgcgtg cataccgaat
    15541 acgccaacaa tgcgctgacg ctgccggatt atttcttcca ccgcttcggc gcgggcggac
    15601 acttgatgaa agtggtttcc gcattgatta tcctgttctt cttcacgatt tattgcgcct
    15661 cgggcattgt ggcgggcgca accctgttcc aaagcctatt tgaaggtatg acttacaatc
    15721 aggcaatgtg gctgggcgcg ggcgcgacca tcgcctatac cttcttgggc ggtttcttgg
    15781 cggtcagctg gacggatacg ctgcaggctt ctttgatgat tttcgccctg attttaacgc
    15841 cggtgatggt ctatctgggc ttgggcggcg cggaacagat gtctaccgcg attcaaagcg
    15901 tcgccgcagg cacgggcaaa gaatacggca gcctgtttgc cggtacgacc gtcatcggca
    15961 tcatttccac cgccgcatgg ggcttgggct atttcggaca gccgcacatt ttggcgcgct
    16021 ttatggcggc cgaaagcgcg aaatcgctgg tatccgcacg ccgcatcggt atgacttgga
    16081 tggcgttgtg ccttgcgggc gcggtagcgg tcggttattt cggcattgcg tatttcggtg
    16141 caaatcccga caaagtttct tctatgagcg gcaaccacga acgcatcttc atcgcgcttt
    16201 ccaccctgct gttcaaccct tggatcgccg gcatcatttt gagcgcgatt ttggcggcgg
    16261 tgatgtccac cctgtcctgc cagcttttgg tttgctccag cgcgattacc gaagacttct
    16321 acaaaggctt tttgcgtaaa aacgcgcaac agtcggaatt ggtatgggtc ggccgcctga
    16381 tggtcttggc gattgccgtg atttccatcc tgattgcttc cgatccgaac agcaaagtat
    16441 tgggactggt gtcttacgcg tgggcaggtt tcggcgcggc attcggcccg attgtgattc
    16501 tgtccgtatt gtggaaacgc atcactgcct acggcgcgtt gtcgggtatg gttgcaggtg
    16561 cgttaaccgt agtcgtatgg gcggaatggg tgaaaaaacc ggctcaagcg gcaggagaaa
    16621 gcggcttgtt gacgatgtac gaaatcgtgc cgggctttat tgtttgcttg atcgttgccg
    16681 tgttggtttc cctgttcaac aaagaacctt cgcgcgaaac ccaagaacgc tttgaaaaag
    16741 ccgacgcaga ttaccgcgcc acccgataaa cccctttcag acggcacggc atcctggttc
    16801 gtgccgtctg aacccgtttc cgggtaaaac acaaataaac cttgcctgca tacagacctt
    16861 atttgtgttt tacccgtttc attttcaaag aaacggaagc acctaccctg cccgcatttg
    16921 aagccttgta tttcatctgc ggatttatta cgatattacc gtattcaggc cgcaccgatg
    16981 ccgcctgccc cccgaaaaac tttggagaat ccaaaaaatg tttcattttg catttccggc
    17041 acaaaccgcc ctgcgccaag cgataaccga tgcctaccgc cgtaatgaaa tcgaagccgt
    17101 acaggatatg ttgcaacgtg cacagatgag cgacgaagag cgcaacgccg cctccgagct
    17161 tgcccgccgt ttggttaccc aagttcgtgc cagccgcacc aaagccagcg gcgtagatgc
    17221 gctgatgcac gagttttcac tctccagcga agaaggcatc gcgctgatgt gtctggcaga
    17281 agccctactg cgtatccccg acaacgccac gcgcgaccgc ctgattgccg acaagatttc
    17341 agacggcaac tggaaaagcc atttgaacaa cagcccttcc ctcttcgtca atgccgccgc
    17401 ctggggcctg ctgattaccg gcaaactgac cgccacaaac gacagacaaa tgagttccgc
    17461 actcagccgc ctgatcagca aaggcggcgc accgctcatc cgccaaggcg taaattacgc
    17521 catgcgcctg ttgggcaaac agttcgtaac cggacagacc attgaagaag ccctgcaaaa
    17581 cggcaaagaa cgcgaaaaaa tgggctaccg cttctccttc gatatgttgg gcgaagccgc
    17641 ctacacccaa gccgatgccg accgctacta ccgcgactat gtcgaagcca tccacgccat
    17701 cggcaaagat gcggcaggac aaggcgttta cgaaggcaac ggcatttccg tcaaactttc
    17761 cgccatccat ccgcgctact cgcgcgccca acacggccgt gtgatgggcg aactgttgcc
    17821 gcgcctgaaa gagctgttcc ttttgggtaa aaaatacgat atcggtatca acatcgatgc
    17881 cgaagaagcc aaccgtctgg agctgtcttt ggatttgatg gaggctttgg tttcagaccc
    17941 tgacttggct ggctacaaag gtatcggttt cgttgtccaa gcctaccaaa aacgttgtcc
    18001 gttcgttatc gactacctga ttgacctagc ccgccgcaac aaccaaaaac tgatgatccg
    18061 cctcgtcaaa ggcgcgtatt gggacagcga aatcaaatgg gcgcaagtgg acggcttgaa
    18121 cggctatccg acctacaccc gcaaagtcca caccgacatc tcctacctcg cctgcgcgcg
    18181 caaactgctt tccgcgcaag acgcggtatt cccgcaattt gccacgcaca acgcctacac
    18241 cttaggcgca atctaccaaa tgggcaaagg caaagacttt gaacaccaat gcctgcacgg
    18301 catgggcgaa accctgtacg accaagtcgt cggcccgcaa aacttaggcc gccgcgtgcg
    18361 cgtgtacgcc ccggtcggca cacacgaaac cctgctcgcc tacttggtgc gccgcctgtt
    18421 ggaaaacggc gcgaactcgt ctttcgtcaa ccaaatcgtc gatgaaaaca tcagcatcga
    18481 cacgctcatc cgcagcccgt tcgacaccat cgccgaacaa ggcatccacc tgcacaacgc
    18541 cctgccgctg ccgcgcgatt tgtacggcaa atgccgtctg aactcgcaag gcgtggactt
    18601 gagcaacgaa aacgtattgc agcagcttca agaacagatg aacaaagccg ccgcgcaaga
    18661 cttccacgcc gcatccatcg tcaacggcaa agcccgcgat gtcggcgaag cgcaaccgat
    18721 taaaaatcct gccgaccacg acgacatcgt cggcacagtc agttttgccg atgccgcgct
    18781 tgcccaagaa gcggttggcg cagccgttgc cgcgttcccc gaatggagtg cgacacctgc
    18841 cgccgaacgc gccgcctgcc tgcgccgttt cgccgacctg ctcgaacagc acacccccgc
    18901 gctgatgatg cttgccgtgc gcgaagcagg caaaacgctg aacaacgcca ttgccgaagt
    18961 gcgcgaagcc gtcgatttct gccgctacta cgcaggcgaa gccgaaaaca ccctgcccaa
    19021 agacgccaaa gcagtcggcg ccatcgtcgc catcagcccg tggaacttcc cgctcgccat
    19081 ctttaccggc gaagtcgttt ccgcattggc ggcaggcaac accgtcatcg ccaaacccgc
    19141 cgaacaaacc agcctgattg ccggttatgc cgtttccctc atgcacgaag ccggcatccc
    19201 gacttccgcc ctgcaactcg tcctcggcgc aggcgacgtg ggtgcggcat tgaccaacga
    19261 tgcccgcatc ggcggcgtga ttttcaccgg ctcgaccgaa gtggcgcacc tgatcaacaa
    19321 agcccttgcc aaacgcggcg acaatcccgt cctgattgcc gaaaccggcg gacaaaacgc
    19381 catgattgtc gattccaccg cacttgccga acaagtctgc gccgacgtat tgaactccgc
    19441 cttcgacagc gcgggacaac gctgctccgc cctgcgtatc ttgtgcgtcc aagaagacgt
    19501 tgccgaccgt atgctcgaca tgatcaaagg cgcgatggac gaactcgtcg tcggcaaacc
    19561 gattcagctc actaccgatg tcggccccgt catcgatgcc gaagcacagc aaaacctgtt
    19621 gaaccacatc aacaaaatga aaggcgttgc caagtcttac cacgaagtca aaaccgctgc
    19681 cgatgtcgat tccgaaaaat ccacgttcgt ccgccccatc ctgtttgaat tgaacaacct
    19741 caacgaactg caacgcgaag tcttcggccc cgtcctgcac gtcgtccgct accgcgccga
    19801 cgaactcgac agcgtcatcg accaaatcaa cagcaaaggc tacgccctga cccacggcgt
    19861 acacagccgc atcgaaggca cggtacgcca catccgcagc cgcatcgaag ccggcaacgt
    19921 ttacgtcaac cgcaacatcg tcggcgcagt cgtcggcgta cagcccttcg gcggacacgg
    19981 tctgtccggc acaggtccca aagcaggcgg ttcgttctac ctgcaaaaac tgacccgcat
    20041 ccccgaatgg gttgccccga ccctgagcca aatcggacag gcagacgaag ccgcgctcaa
    20101 acgcctcgaa gcactggttc acaaactgcc gttcaatgcc gaagagaaaa aagccgcagc
    20161 ggccgctttg ggacacgccc gcatccgcac cctgcgccgt gccgaaaccg tccttaccgg
    20221 accgaccggc gagcgcaaca gcatctcatg gcacgcgccc aaacgcgttt ggatacacgg
    20281 cggcagcacg gttcaagcct ttgccgcact gaccgaactt gccgcctccg gcatacaggc
    20341 agtggtcgaa cccgacagcc ccttggcttc ctacactgcc gacttggaag gtctgctgct
    20401 ggtcaacggc aaacccgaaa ccgccggcat cagccacgtt gccgccctgt cgcctttgga
    20461 cagcgcgcgc aaacaggaac ttgccgccca cgacggcgca ctcatccgca tcctcccttc
    20521 ggaaaacgga ctcgacatcc tgcaagtgtt tgaagaaatc tcttgcagcg tcaacaccac
    20581 agccgccggc ggcaacgcca gcctgatggc ggtcgccgac tgattttgcc gaaatacccg
    20641 ggcgcggccc gtgaaccaat gccgtctgaa aacctttcag acggcatttt tata
//
LOCUS       DAXHNE010000020        19790 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.020, whole genome shotgun sequence.
ACCESSION   DAXHNE010000020 DAXHNE010000000
VERSION     DAXHNE010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19790
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.020"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(203..278)
                     /locus_tag="ACFM4Z_000568"
     tRNA            complement(203..278)
                     /locus_tag="ACFM4Z_000568"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:Asn,seq:gtt)
     gene            complement(371..730)
                     /locus_tag="ACFM4Z_000569"
     CDS             complement(371..730)
                     /locus_tag="ACFM4Z_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HFC6020654.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(753..1397)
                     /locus_tag="ACFM4Z_000570"
     CDS             complement(753..1397)
                     /locus_tag="ACFM4Z_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFC6020655.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1399..1935)
                     /locus_tag="ACFM4Z_000571"
     CDS             complement(1399..1935)
                     /locus_tag="ACFM4Z_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HFC6020656.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1947..3044)
                     /locus_tag="ACFM4Z_000572"
     CDS             complement(1947..3044)
                     /locus_tag="ACFM4Z_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6020657.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3047..4483)
                     /locus_tag="ACFM4Z_000573"
     CDS             complement(3047..4483)
                     /locus_tag="ACFM4Z_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6020658.1"
                     /translation="MENSLIIGLALAVLLVLLVMRAKQGKKTPKRKSQGVGNTQGQPP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEVEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQYADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="ACFM4Z_000574"
     CDS             complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="ACFM4Z_000574"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFC6020659.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVRLTYHPLFFIARYSAAGRQ"
     gene            complement(5473..6120)
                     /locus_tag="ACFM4Z_000575"
     CDS             complement(5473..6120)
                     /locus_tag="ACFM4Z_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl esterase BioG family
                     protein"
                     /protein_id="HFC6020660.1"
                     /translation="MKTKFYNYQGEHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFPARPFDEIHQELIALFAMIGQDRR
                     ADLIRWTNALVGSGDKIFMPANQHRYWTARCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="ACFM4Z_000576"
     CDS             complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="ACFM4Z_000576"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFC6020661.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENCKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFSRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPPTVPKGTSRIRLSLTADMTMDEVRQFAACL"
     gene            7495..8570
                     /locus_tag="ACFM4Z_000577"
                     /pseudo
     CDS             7495..8570
                     /locus_tag="ACFM4Z_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            complement(8777..10243)
                     /locus_tag="ACFM4Z_000578"
     CDS             complement(8777..10243)
                     /locus_tag="ACFM4Z_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC6020662.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10441..11364)
                     /gene="speB"
                     /locus_tag="ACFM4Z_000579"
     CDS             complement(10441..11364)
                     /gene="speB"
                     /locus_tag="ACFM4Z_000579"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFC6020663.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11463..13355)
                     /gene="speA"
                     /locus_tag="ACFM4Z_000580"
     CDS             complement(11463..13355)
                     /gene="speA"
                     /locus_tag="ACFM4Z_000580"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFC6020664.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPAQHNQTASLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13522..13806)
                     /locus_tag="ACFM4Z_000581"
     CDS             complement(13522..13806)
                     /locus_tag="ACFM4Z_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC6020665.1"
                     /translation="MKKLLLAAVVSLSAATAFADDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13957..15765)
                     /gene="aspS"
                     /locus_tag="ACFM4Z_000582"
     CDS             complement(13957..15765)
                     /gene="aspS"
                     /locus_tag="ACFM4Z_000582"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFC6020666.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSSRNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALGVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIERTGAQ
                     NGDIIFFGADKTKVVNEAIGALRIKVGLEHGAENGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKVAETKEA"
     gene            complement(15823..16524)
                     /locus_tag="ACFM4Z_000583"
     CDS             complement(15823..16524)
                     /locus_tag="ACFM4Z_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HFC6020667.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16667..17815)
                     /locus_tag="ACFM4Z_000584"
     CDS             complement(16667..17815)
                     /locus_tag="ACFM4Z_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFC6020668.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(17975..18238)
                     /gene="rpsT"
                     /locus_tag="ACFM4Z_000585"
     CDS             complement(17975..18238)
                     /gene="rpsT"
                     /locus_tag="ACFM4Z_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFC6020669.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18541..19683
                     /locus_tag="ACFM4Z_000586"
     CDS             18541..19683
                     /locus_tag="ACFM4Z_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFC6020670.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5091 a         5118 c         4593 g         4988 t
ORIGIN      
        1 caatgccgta ctggtttttg ttaatccact ataaaatcga gtttatccta gctgtccagg
       61 acagccccta ttttttcata accatcacga aaggaatttt gcaaagattt tattccatct
      121 caaaacaatc atctcaaaaa tgcgtttctg accgccggta aaacaaaacc ctctaagaaa
      181 atacttagag ggttttgaaa tttggctccc cgacctgggc tcgaaccagg gacctgcgga
      241 ttaacagtcc gtcgctctac cgactgagct atcggggaac aagcgcgaat aataaaacag
      301 aaataccgaa cgtgtcaata taaattgaca atcgattgca ctttatctgc aagcccgcaa
      361 tctttatatg ttatatggct tccgcaatat gccttgccgc acgtttggca tccaatagga
      421 ttaaaccaag tctgccggtt tcttttgcca ccagcaccaa cacggcatct ttacccgcct
      481 gacttaaaag gatatagcct gattttcctt taatcatcac ttgttccaat tccccgcagg
      541 cgagttcctg caccgagcga ctccccaaag caagcaaagt ggcagaaatc gcccctaccc
      601 tgtccgaatt caaatgtgaa ggaagcattg tcgccatcgg caatccgtcg gttgagataa
      661 cggcagacgc gataatatcc gtagatgtat tgtttaaatc ttcaaggatt gaaatcaata
      721 attgctgcat aacccctcct ctctcccaag ttttacacgc ggttgctgta acggcggtat
      781 aaaatcctta ccaaagtaac aaatgcctct ttgcccaaat cgggaatgcc gccgataacc
      841 aaaataaatt tggttgaacc gatatacaat gggaaaaatg tcaattcgct ctgaccggaa
      901 ggatcgcaaa cgccccaagc gttattgttg atatacaggt tgttcttaat cagcagccgg
      961 tatttctttt ccatctgtgc gacttctgcc gccaacaacc ccaactcttc cgccgcctca
     1021 tgatggaaat tggcgttggc aagatacaga ccgttccgat cgaccaataa cgccttaccg
     1081 ctgccggaca attgttccat cagcaacggc aattgctcgt ccgacaaatt gatgccgtct
     1141 gaatgaccgt tttcatcgcc atagaggaat tcgagttttt gcaaacggta caacaggttc
     1201 aaagcggtat cgatgtcggc ggtgtccgcc caggtaagca gcttctcact gctgaccatt
     1261 tcgtccgcat ccgctttcaa caggctgtgc aacaaagttt taccggcact gggggcatcg
     1321 ctggatacgg cataaaatgc accggcagga gtcaggcggg gatataaatt tgcttgtagt
     1381 gaaagtgttg attccatatt aaacctccag tcccggatca atagaaaata acattgcgct
     1441 aaccaattgt tttacgtcat cttccttacg ggcatcaatt tcaaaaaccg gaacattaag
     1501 attatgtttt gcaagatatt tgtgatacac gtcgataccg ggctgagagc gtatatccat
     1561 cttggtaata ccgacaacga cgggtgcctt ctccagcagc cctcgaaacg aatgtaaaaa
     1621 gaattccaaa tctttcaacg gattggttcg ggcattatct aaaagcaaga ccaaacccat
     1681 actgccttgg cttaagattt cccacataaa gttgaaccgt tcctgaccgg gcgtaccata
     1741 taaatggact ttggtatcct catccaagct gatggccccg tagtccatcg ccactgtcgt
     1801 attccttttc ctatccaaag tcatatcgga tgcggaagca tcggtctgaa cgagtgcttc
     1861 gtccgaaata gccgcaatgg cagtggtttt ccctacgccg acaggtcctg tgaaaataat
     1921 tttattttct ctcatctctc cgcctcttag ctgcccaaca gttttttcat cagcctttgc
     1981 agaaggccgc gcgactggga ttgggatggt gttgtgattt tttccgcttt tttcatcatt
     2041 tcactatcag aggcagaatc ggctccgata tttattttat ccgccatatc ggagtatgcc
     2101 tgttgtgaaa ccgtttttaa atctaccgac aaaaacccgg ttgtataggt tgccgcaaga
     2161 taattcagaa tatcattgag gtttaaaggc atcactttat acaacacgtt aaggttgacg
     2221 gatgccttgg tcagaaatgc cgacaagcgt atcgaccccg gcacatttgc caaccgggtc
     2281 aggtttggcc aagatttcaa cgtaaacgga gtatcgggag aaatcggata aatcaacctg
     2341 ccctgcgctg tccaaatgga aaactgccac atacaggaca taatgcctac tttagccttt
     2401 tcgcgccatt gcgggttatc gggaacagtc ttgcagctga cctgcaaatt ttcgtctttg
     2461 cacaattctt cgagtttttg cacactttct gtcagcaaaa cccgttgtat cgaggggaaa
     2521 acaataagga ccggcttatt tccatgcaag atagcgatgt cctgcctgtt cttttccgca
     2581 aaccgcaacg cccccaataa tcctttattc gggttaaact ggcgtatcgt aaccgtacgc
     2641 tgcacattcc cgttattttt cgccgaccgg tctgcgggtg caataaacga tttcccataa
     2701 acattctcgc cctgcaacaa tttgcggagc ataggaaaca atgtttcaaa ccgaatcggt
     2761 ttgggcaggt agggaacttc agaatcggga actttctccg aacagacggc gacgggtata
     2821 tccttataac gctcggcaag ctctttccaa agttcaaaac cgccctcggc atcggtatcc
     2881 gccaaaacca aatcgggcac ggcactgccg tctgaagggg atactgtttc ataacgggtg
     2941 gtattgtgca ttttgaatgc cattttgaaa acggattcct gctgcgccgt catccccgcc
     3001 aacattacgc gtactgtttt aattttcggc agttgaactt ccatttttat tttccgtacc
     3061 gttttatttt tttaatattt tgattcatac gctgcagcag ccggctcatc agcataacga
     3121 cctcttcagg aagcctgtcc gcacgttccc tcaataccct taaaaactgc cccaacctat
     3181 cccaatcttc agtacgttca taaatatcga tcaacgtaat ataaagctgg gactcgtcgg
     3241 gatatttcaa taccgcctgc tccaacacat ccattgccgc ttcaatctga ccatacatca
     3301 gcaacgactc tacttcctta accgcatcgt ctgccggaga cgaaccggtg ttgatcaacg
     3361 aagaatcttg aagcaccaaa tcccgatgtt gcgatttgaa tttctgtata tttttcggca
     3421 gatacccgtg ccccataccg atgtctttga tttgccggtc gttcggccct ttttccaaat
     3481 catcgaaaac ctcatggtaa cccaggctgt acccccaccc cagcatccgc tctttaacct
     3541 gcctgccgta gttgcccaac gtttggtaaa gtttccacaa atgtccggca aaacggtcta
     3601 tgtccgcgta ttggtaatcg agtttcaacg catcgataat caggtttgca ggtttttcgg
     3661 aagtttggat ggcacggtcg tattgtttcg atgccgtttc atagctgact ttgtctttaa
     3721 ggattttcgc accttgatcg gcacggacca aaccggcaat cgcaccgatt tcctcttgac
     3781 tgattccgga cacgtctttt ttgccccgca caatcgggat gcgcttgatt tcttcgactt
     3841 cataagcctt accgccggca tccggcgggg gcgatgcgga agctgccgtc gcagaaccgc
     3901 cttccgcacg tttttcaatc tcctgagtac cccatcccaa accttcttcc gccaagacgc
     3961 ggatacgcaa atggtttgaa tcgcgctgta atgcctgttc gatatatttt gccaaaagtt
     4021 cggaaggaat cagtttgccg tatttttgca gattgtctgc caaaacatcg acatccccca
     4081 cttcgagatt gatatcgagc agctcgcgga taaggttttc aggtttcgct tcaccatccg
     4141 gaatgccgtc cagataggca gccaaagatt cggcagcctt gccctgataa ccgaattgct
     4201 tataaacctg atactccgta agcggatcga cttcttgcgc ggatacggcg gcggatgcgg
     4261 tctcggcact ttcgttccaa gaccagtcgg gttgcgtgcc gtctgaaacc gattgtccaa
     4321 tctgatcgac ccagtccgaa tcattgcttc cgggcggctg cccctgcgta ttgccgacac
     4381 cttgggattt gcgcttcggt gttttcttac cctgcttcgc acgcataacc aaaagcacca
     4441 gcaataccgc cagtgccaga ccgataatta atgagttttc caaaggatac tcccgatacc
     4501 gtaacgagcg gatagctgac cgcctgcaac ggccattgtc cgcttttatt attaacgatt
     4561 tactgaaata gacttgtaag ttttaaatca taccataatt taacgtttaa caatatgcct
     4621 tctgcacaag cctcgccata ttacctttta cccacatcag tatcaatacc cgatataaaa
     4681 ataactttgc ccataagctg ccttattgcc tgcccgccgc agagtagcgc gcgataaaaa
     4741 ataacggatg ataagtcagg cgcactttgc ccgacggcat actgaacgcc gacaagtaat
     4801 cgccaataaa tccattgaga ttttttcttg tccaattttt ttggttcgtg gctgttacgc
     4861 ctgtatattt aaggtgtttg agtacatcta acggcgtatc aaagtctagt attaccttaa
     4921 aatcctcaca ccataaaagc tcaaaatctt tggctaacca agtctgccat tgggataaag
     4981 tcgggtaatt taagcctata tttgtaattt ggcggacttc ttttaaattg tctttgccaa
     5041 aggttgcaac cgccaataat ccgtttgctt tcaagcctgt tttgcaatgg gcgataaaag
     5101 cgtcgggttg atgaaaccat tgcacggcag atgcgcttgc gattaagtca aattgtcgtt
     5161 gaaaaggaaa gttttccgca tcgccgcaat aaaaatcaaa ggattgcggc agtttttcag
     5221 ccagttgggg ctgcacatcg cacaaatcat taaataacca ataattcgct gaaatctgtt
     5281 tttgcagcaa ggcactcaac attcctgagc cgcagcccaa ttccaacacg ttttccaatg
     5341 gcatatccgg caaataatct tgcaaatgcg ccattagatt aatcgtcatt ttttgttgga
     5401 ttaaggcgtg ccggtcataa tcgtttaatg ctttttggaa agcctgccga atgcgcgatt
     5461 tatttatggc agtcagtgat tccatagtgc cgaccaatgg gtgaatcttg aaaacaggta
     5521 atgtccgccg tcaatttccc gaacggtgca acgcgctgtc caatatcggt gctgattggc
     5581 aggcataaaa attttatcgc ccgatccgac caaggcattt gtccagcgaa tcaaatctgc
     5641 gcgcctatct tgcccgatca tcgcaaaaag tgcgataagt tcttgatgaa tttcgtcaaa
     5701 cggacgggcg ggaaattgtt ggtaatcttc aaaagatgct ttatcgccac acattctgcg
     5761 ttcaaatttt aaacgggtgt tttccgtgag gttctccaat gtgcctttaa aaacggcgca
     5821 agggataccg aaattatcat cgcaaggcaa acctgtgcca ttcactgccg ttgcggattt
     5881 taatcttatt ccttgcaatg ccctctctgc cgcccaaacg cccattgacc acgccaccaa
     5941 acggatgtgc cgataggcgg aaaaatcaaa atccaaattt aaatcttgat aatcatagca
     6001 aatcaataaa tcgtgatttt ccggcaaaat caaatgattt acagcatcgg gcggcgttcc
     6061 ccaacctgca aaatacagga ttaaatgttc gccttgataa ttgtaaaatt ttgttttcat
     6121 atcatatcct tacaggcacg ccgcaaactg ccgcacttca tccattgtca tatctgccgt
     6181 caaagacaat cgtattctgg atgtaccttt tggtactgtc ggtggtctga tgggcaggca
     6241 ataataaccc tgcctttgca ggtattccgc tttggcaagg gtggcttcat tcccgcctaa
     6301 aatatagggg acgatacagg tttggctcgg cattatttgc gtccgatgcg ccacttcccg
     6361 ccgtaaaaat gcgcttaact gctcaagatg gcttctttct tttgagaatt gcggcaatcg
     6421 ttcaaaaata aaataagtcc aagccacatt aaacggcggc aatgcggttg aaaaaatcaa
     6481 tgggcgcatt tgattaatca aacattcttt caatacttgg ttgcagacgg catacgcccc
     6541 caccgaggct aaggctttac cgaaagtgcc gaccaataaa tcaatctcgg caatcaaatt
     6601 atcccgttcg gcaatcccca atccgttttg cccataaaca ccgattgcgt gggcttcatc
     6661 cacataaaga taagtattgg gaaactgttt ttttaattgg acaagctgtt tcaaatccgc
     6721 cacatcgccg tccatactga aaacagattc ggtaacgata aaagtgcggt caaattttcc
     6781 gacgtttttt tcaagcagat ttttcaaatg ttcataatca ttatgacgat aacgggaaaa
     6841 cgcacaccgg ctcaaacgga tgccgtcaat catactggcg tgaacaaatt tatctgccaa
     6901 aatcaaactt ttcgtcgtcg tcaaagcagg caaaataccg agattggcgt gatagccgct
     6961 gttgaacaat aacgcgcttt cccgttggaa acgttgtgcg acaagctctt ccaaatcggt
     7021 ataaatagga aagttgcccg ttaataaacg cgatgaagaa ctggtaaaag agggaaaatt
     7081 accgccgtat tgctgcaaaa aagaccggcg caagttttca tctgatgcca aacccaaata
     7141 atcattagac gacatattca gcattttgca gttttcccgc gtaatatacc gcccttgatg
     7201 aatcaaatcc ggaatcgaac gatattggtt ttgcgcgccg agttgttcaa gctgttgttt
     7261 aaaaaccttc attatgatgt aaatattcct gagttaaagc ctgaaccaac ccagcatcaa
     7321 acaatcaagt aatgcccttt taataaaaac cgttttgccg ccgacctttg ccacacccct
     7381 gccttcataa tccaaggcgg aaatttccgc gacatttgtt tccgttgcca ttacccaatc
     7441 cgtccgcact gtaccgaagc gcatattttc gcacaaattg gtacggtttc aggtatgatt
     7501 tgccgaaaac ctatcggaca cacaccatat tttgatatga ataaaacaat ctgccgcact
     7561 gctgcactat tgataagcgg tttttcatac gcaaacaccg tcattcccga tgtctccccc
     7621 gtcgcacaag gtcaacatgt cttcattaac atccctcagc aacgcctgtt cctctacacc
     7681 gacggcaaac tgaccaaggt ttatcccgtt gcagtaggtc gggcgatgac gcaaaccaat
     7741 ctaggcgaac ataaaatcgg tgcgaaagcc tataaccctg tttggtacat ccccaaatcc
     7801 atacagaaag aacgcggcga cggcgtaaaa accatagccg ccggcccgga caaccctttg
     7861 gggccggttt ttgtccgctt aggcgacccc aaactcggtt tgggtataca cgggaccaat
     7921 gcgccggcca gcgtccccgg ggttcggagt cacggctgcg tccgcatgaa atcgcccgac
     7981 gcgctcgagt ttgccaaaac catcgccagc ggctcgcccg cctccgtcat ctatcaaatg
     8041 gcgggtctca atgaagatgc ggatcgcaac ctgtggcttg ccgccttccg cgacccttac
     8101 ggtaagaaca accttgacat cgcctctctg aaaaaagtat tgcgcaatgg gcaaaaacac
     8161 agggtaaaac catcgcgccc gagaaagtcg atgctgtact caaagaccgc accggatcgg
     8221 ccgtctgcct gacctgcggc aaaaacggca agatgaagat gccgctcaaa tcgctggcgt
     8281 ggatacaggg ttcttcctca tacagccaag ctgaagtcat tgaacaaact gaggaaacca
     8341 actctgccga aatatccgag acacgcacgc ccgaagtgcc tgatgtacac acgcccgagg
     8401 cacaaccgca tttaaatacc caatccgacg gcacgccgac tgcctatacc gaaccggctg
     8461 ccgattcatc gccgcaagta gaaacacctg atcaggctgc ttccgagccg gttgatgtat
     8521 tattttcaat agatgtgata cggcagggaa atttgcgttt aggtaattga ataaccttct
     8581 gattatttaa tgcttatgtt tatcagaagt tgataggcgg tttggttttt gtttggcttt
     8641 tcctacaccg ccgcctgccg ctttctgcaa cattcaagcg cacaaatatg ccatctgaag
     8701 gctttagacg gcatatttca cgataatagt cgcccaatca aataaacaca gattggaaat
     8761 ttattttatt tttcccttac caatacccca gaactttcca ccaaatgctg ccgataacga
     8821 aaaaaatcag aaaattgact acgctcatga taaaacccgc cttccaccat tctcccattg
     8881 tggtgtagcc cgaaccgaaa atcacaggcg aagtaccggt cgcataatga gtgagggtca
     8941 tcataatgtt ggatgcggcc gccatcatca gcgcggtcgg catcgccggg gcattcagtg
     9001 aaacggcagc agcgaaaaat gcgccgaaca tagcggtaat atgtgcagta gtactggcaa
     9061 acatataatg cgcatacata taagcaagca cgaggattac gcccgcagcc gtgccgctaa
     9121 cgcccaaacc gccgacactt tccgccaaca ctccggagaa ccatttaatc agtccgagtt
     9181 tatttaaaaa tgcggccatc ataatcaatg cgccaaacca aataatcgta tcccacgcgc
     9241 ttttttcttt caaaacatcg tcccaagtca atacaccgga aagcaaaagc aggcttaatc
     9301 cgataaatgc ggtggcggtg gcgttgatac taaaagcgtg attgccggta ataagggcgg
     9361 gaacatctgc ccacaacagc agcaagatac cgaaaatgac cgccataatg atttcgtctg
     9421 ccgacatttt acccatctcc ctcagacggt ctttggcaaa ttgaacggca ttgggcgttt
     9481 ctttaatttc aggcggatac aaaaaatata aaatcaaagg cataacgaaa aaggcgataa
     9541 cgccgggaac agccattgcc cacgcccacg ccccccaaga aagacggaaa ctactgccta
     9601 aattttcggc aatcaagttg acgattaaag ggttgggggc agttgcagta ataaacatag
     9661 ccgacgaaat gggattggaa tgatagttga ccaaagccaa atatttaccc atcttgcctt
     9721 ctgtgccttt tgcgggattg gagccgtaac tgccggcaat cgactgcata atcggatgta
     9781 taatgccgcc gccgcgcgcg gtattggaag gggtaacggg agccagcagc agttcggaaa
     9841 gagcgagact gtaaccgatg cccagcgttt ttcttccaaa aacggcgata aacaaatatc
     9901 cgatacgcat ccccagccct gttttgagca aaccgcgcga aatcataact gcgatggcaa
     9961 tcagccaaat caacggattg gcgaacgcac tcaacgcatc gctcatcgcc gcgcccggtt
    10021 tgtcggcggt tacgccggtt actgcgacca acccgacggc aataatcgac agcgcgccca
    10081 acggcatggc cttgccgata atggcggcaa tcacaccgac aaacatggcc agcagcgtcc
    10141 aagcctgagg cttgaccccg tcgggtacgg gcagtgccaa aaccagggcg cacaatactg
    10201 cggcaatggc gaggggtatc ggtttgaaac ccaatttcat catattgacc tccgtaaaaa
    10261 agaccgtccc gaaaaatcgg aaaaataata tttaactaat tgttttataa gatatattct
    10321 gatatttcac cgtctttccg atatgcggct ccgggcaact tttgtttcag tatttgaatt
    10381 ttcattagac tgaatacgcc gtttgaacgg cacggcgaaa acccggggga tgccggacgt
    10441 tcagtccttt ttcgcacctt gaaggtaaag catttccaag gcaattgtgg cgccggccaa
    10501 agcggtaatg tcggattggt cgtaagaggg ggcaacttct acaacatcca taccgacgat
    10561 gtcgagatcc gtcagcccac gtaggatttt taatgccctg tcgctgctca agccgccgca
    10621 tacgggcgta ccggtcccgg gggcgaacga cgggtcgagg cagtctatgt cgaaagtcag
    10681 gtaaacgggc atattgccga cggtttcttt gattttacgg acggtctctt caacactgtc
    10741 ttcattgact ttaggggcgg acaacacagt aaaaggcaat tttttactgt gttcggtgcg
    10801 tatgccgatt tgtacggaac gggacgggtc gatgaggcct tccttggggg cggtatagaa
    10861 catcgtaccg tggtcgtatt cgctgccgtt gtcgtaggtg tcggtgtgcg cgtcaaaatg
    10921 aatcagtgcg agtttgccga aatagcgggc gtgggcgcgc aacaacggta gggtaatgaa
    10981 atggtcgccg cccaaactca aacagcgttt gccggaagaa agtaatttgc cggcgtgcgc
    11041 ttccattttt tcgacaaaat ccctgctgtc gccaaaagaa aaaaccaagt cgccgcaatc
    11101 aataatgttc aggcgttcgc gcacatcaaa tgtccatgga aacctgcggt gctcccaagc
    11161 gaggttgacg gaggcgcgcc ggatggcttc aggaccgaaa cgcgcgccgg aacgccctga
    11221 aaccgccata tcataaggca cgccggtaat aacccaatcg gcatgacttt catacggcat
    11281 aaaattaagc ggcaggcgca aaaacccgaa attattggaa acgagggagt tgtcggtttg
    11341 tcctgccagt gtgctgtatt gcatcgtaat gattccttgt aattggtttc aatcggtcgt
    11401 gatgattggt gtttgagtaa gaaaatcggg cttcagacga catatccgat gccttgatgc
    11461 gtctattcgt cttccaaata ggtataacca ttaagccccg cttcgagttc ttttaagaaa
    11521 gacatagcct gcgaggcagg aaggtctgaa tgttcgattt gttcgcgata gcgtttcatc
    11581 agctctttcg gatcttgata aacgtattcg agcatatcgg caacggtgtt tccttcatcg
    11641 taatcgatga cggtaaattg tccgtcttcc cctacaacaa catcggcagt ggcagtgtcg
    11701 ccgaaaagat tgtgcatatt gccgagtatt tcctgatatg ctcccaccat aaaaaagcct
    11761 aaaagcggcg gctcttcttc gggataatca ggcataggca tcgtaccggc gatgccgtct
    11821 ccgtcgatgt agtggtcaat cgtaccgtct gaatcgcagg taatgtccaa caacacggcg
    11881 cggcgcgcaa tcggttcatt caaaccggta atgggacaaa cagggaaaag ttgatctatg
    11941 ccccaagcat cgggcaaaga ttggaagagt gagaaattga catacagctt atcggcaaaa
    12001 cgttcttgca attcgtcaat aatggttcgg tgagaccggt gtttttcatt aaacaattcg
    12061 ccgacttcat gacagatatt taaatacagt tgctccgccc acgcacgttg cgccaaactc
    12121 aacagcccga cattatactg attatgcaca tcagcaagat caaactgccc ttcgtgtatc
    12181 cagctgcgta aggaacgttt ttcccgcgag gcggaaatat ccgtccaagt ttcccacata
    12241 ctgtgcaaca cacgcggtgc ttcgggcgat ggcgcatcca gccggcgcgg tttgtaacgt
    12301 tcaacgccta taacattagc aaccaaaacg gcgtgatgtg cggtaatgcc gcgcccgctc
    12361 tcggtgatga ttgtcggatg cggcagcccg tgttcgagac aagcctgact gatgccccat
    12421 acgactgtgg cggcatattc gttgaggctg taattaacgg aacaatccga ttgtgtgcgg
    12481 tttccttcgt aatccacgcc aagcccgccg cctacatcaa aacagcggat atttaccccc
    12541 agtttgtgca actcaacata aaaccgagcc gattcgtgta cacctgtggc aacatcacgg
    12601 atgttcccaa gctgcgagcc caaatggaaa tgcaaaagct gcaggcaatc cagcctgttt
    12661 ttttgtttca aaatatcgac cagttgcaaa acttgggaag ccgacaagcc gaattttgat
    12721 ttttccccac ccgaagactg ccattttccc gaaccttggg aagccagtct ggcgcgcaca
    12781 cccaaacggg gcttgatgcc gagtttttcc gcctcttcca ataccatttg tatttcggac
    12841 agcttctcaa tcaccaaata aacctgatgc cccagttttt cgcccatcaa ggcgaaacgg
    12901 atatattcac ggtctttata gccgttgcag acgattaatg tttgccgggt gccggcgtgt
    12961 gccaaaaccg ccatcagttc ggctttagaa ccagcttcca aaccatgcgg ttgtccgctt
    13021 gacataagcg attcgatgac gcggcggtgt tggttgacct tgatagggta aaccaaacaa
    13081 taaccgccct tatagccgca ctcttcccgt gccgtctgaa aggcgcggtt aatgtcgcgg
    13141 aggcggtgtt cgaggatttg cggaaaacaa aacaaaacag gcaggcgcgc ctgatgtttt
    13201 tgttgcacgg cttcagtcag tttttgcagt gaagcagttt gattgtgttg cgccggattg
    13261 gggcggacga tgatttcgcc ggaatcgtca acatcataat aacctatgcc ccaatgatta
    13321 atgttgcaca cttcacggat ggtaaggata ggcataataa acctgctccg tctgtcgtgt
    13381 tgaaaaggaa tgattataac aaatcagcgt gaaatgtcat ttttttaata agaaaagcct
    13441 gcctcatacc tgatgaggaa caggcaaaat gccgtctgaa cgcttcagac ggcattttgg
    13501 ttcatctttc catcaaagga gtcagcgatc gagctgctct ttgatgattt tcaggtcggg
    13561 gtaagacaac acgatgtcgt attcgcggcc gtctttatag gcttccactt ccaaaacagg
    13621 tttgccccaa tggtcgtcgg catcgacatc gtgaacctga taaccgcgct gttccaacat
    13681 ttttacggct tttgtgcggt tttgttcaaa atggggatca ccgtaaatct gacgctcggc
    13741 agaatcgtcg gcaaatgcgg ttgcggcact cagggaaaca acggcagcca ataacagttt
    13801 tttcattttc agtcctttct taatcggttg attgaataag atgtgttttt caatgctgcc
    13861 attaaaacac agcaaaatta ggtttgaatt agcaggaagt taagaagcgt aaatgccgtc
    13921 tgaaaaaaac agagccgtca aacggctccg ttttccttat gcttctttgg tttcagccac
    13981 cttttgacgc aggcgcaagc tcaattcgcg caactgcttg tcgtccacgc tgttgggcgc
    14041 gttggtcagc aggcattggg cacgttgtgt tttcgggaag gcaatcacgt cgcggataga
    14101 ttcggcgccg gtcatcaggg taaccaaacg gtcgaggccg aatgcaaggc cgccgtgcgg
    14161 aggtgcgccg aatttcaggt tgtccaagag gaagccgaat ttctcttgtt gctcttcagg
    14221 gctgattttc agcgcggcaa acactttctc ttgtacgtct gcacggtgga tacggataga
    14281 gccgccgccg atttcccagc cgttcaatac catgtcgtag gcgcgtgcca aacaatttgc
    14341 cgggtcggaa accatcaggt cttcatgacc ttcttttggc gcagtaaacg gatggtgtac
    14401 ggcaacgtag cggtcggctt cttcgtcgta ttcaaacatc gggaagtcga caacccacaa
    14461 aggtttccat tcgtctgtga agtagccgtt ttccgcgccg tgttctaaac cgactttaat
    14521 acgcaatgcg ccgatggctt cgttcacgac tttggttttg tctgcaccga agaagataat
    14581 atcgccgttt tgtgcgccgg tacgctcgat gatttctttc agggcattct cagacaggaa
    14641 ttttacgatt ggagattgca ggccgctgtc ttcgccgttg gaaaggttgc ccacatcgtt
    14701 tactttgata tatgccagac ctttcgcacc gtagatgccg acaaatttag tgtattcgtc
    14761 gatttctttg cggctgaatt tcgcaccgtt cggcacacgc agggcaacca cgcggccgcc
    14821 tttcatgtcg gctgcgccac ggaagacttt gaattcttcc gttttcatca ggtcggtcaa
    14881 ctcggtgaat ttcaagttga tacgcatgtc cggtttgtca gagccgtagt agaacatggc
    14941 ttcagagtaa ggcatgcgtg ggaagtcgcc caagtccacg cccagcgcgt ctttgaagac
    15001 ttgtttcgcc ataccttcgg tgatgtccat gatttcatcc tcgtttaaga acgaggtttc
    15061 caagtcgatt tgcgtgaatt caggctgacg gtcggcacgc aagtcttcgt cgcggaagca
    15121 cttggtgatt tggtagtaac ggtcgaaacc cgccaccatc aacagttgtt tgaataattg
    15181 cggcgattgc ggtagcgcga aaaactcgcc cggatgaacg cggctcggca cgaggtagtc
    15241 gcgcgcgcct tcaggcgtgg agcgggtcag catcggggtt tcaatgtcga tgaaaccttg
    15301 cgcgtctaga taacggcgaa cgcccatagc gacttggtaa cgcaggcgca ggttgcgctg
    15361 catcacaggg cggcgcaggt cgataacgcg gttggtcagg cgaacgtttt cgctgatgtt
    15421 ttcatcgtcg atttggaacg gaggcgtagc ggcagcgttc aacacttcga tttctttggc
    15481 aagaatctcg attttaccgg aaatcatttt gtcgttggtc gtgccttcgg gacggttgcg
    15541 tacgcggccg gtaatgctca aaacgtattc gttgcgggag gaatcggcag cggcaaacgc
    15601 ttcgggcgtg tcgggatcga tcacgacttg gacgatgcct tcgcggtcgc gcaggtcgat
    15661 aaaaatcaca ccgccatggt cgcgtcgacg gtgtacccag cctttgacgg taacggtttg
    15721 gtctaagtat tgctcgctaa tcaggccgca atagttggta cgcataaaat caccttttat
    15781 tgatttaaac tgaaaacaga aaatgccgtc tgaacggcgg ctttattgtt gttcgggcaa
    15841 atccgccttt tcagacggca taggtcctgc caatgttttg acgggcaggt cgtcagggat
    15901 gaccataccc agcgaaatca catatttcaa cgcttcgtcc acgctcatat cgagttcgcg
    15961 cacatcactt ttctttacca taatatagta accgccggtc ggattcggcg tggtcggaac
    16021 atacacggaa agataatcgc cgtccttcgg caatgcggcc ttaaccgcat tcgacacctg
    16081 accggacacg aatgcgattg tccaaatacc cgattgggga aacggcacga gtactggtgt
    16141 tttaaacgaa cggctgctgt cggacagcaa cgattcggat acttttttca cactcgaata
    16201 gatggacttc acaaccggaa tccgccccaa caggctgtcc cacgcggcaa gaatctgccg
    16261 gcccaatacg tttgcggcaa acaatccggt tacaaacaat acggcaatgg caacaataac
    16321 gcccagcccc gggatattaa accccaaaac atattgcggc cgccattgct tcggcagcag
    16381 gttgacaagc tggtcggacg cggaaacgat ataggaaacc acccaaaccg ttaccgcaat
    16441 cggcagccag accaaaatgc ccgtaatcag atattttttt aacgccttgg cagctttgcc
    16501 gccttcggcc gcaggttccg tcatcttgct tgattccgac aaagtccgta caaaccgcac
    16561 attatacgcg tttgcccgga ttcaaacgaa atttttatcc cgccccgcca aaccgccggc
    16621 ggcttcagac ggcacggcaa cttgatatat cgtctgaaca cgcggttcag atgccgtcca
    16681 agtcgttgaa catcaacccg ataccgatac cgttctgctt gtggttgtag tcgatcaggc
    16741 tctcgccgta accgtggaat ccgcgtacca cgcctttgag tttgccctta atcggaaacg
    16801 tgtaggcggc ttcaatcgcg ccgtagcccg ttttggggtt gtagcgcaat acggaataca
    16861 cattctgcct gtcgttcagg cggtactgca gcttcacgtc gccatacccc atatagtcgg
    16921 caatatcggg attgtcgttt ttatcgccgc tctgatcgaa cgcacgcacc cacacgcgcg
    16981 gaatcaccgt caatttgccc cattccatgc ctgccatggc gtaaatcctg ttccacgaac
    17041 gcgattcggg acggctctgt ccgttggact ggtggacaaa acccgcaccg agcatacgca
    17101 gcctgccgcc gaacggcaaa tccgccttca caggctgggt caggaaaatt tcaggtttgt
    17161 aatccgtatt gcggaacggc gcggatttcc tgccttggtt gtaaatctgc caatcggatc
    17221 tttgggtgta gccgaaccac agatccgcgc gggttttaaa caaatcttcg gcaattttgc
    17281 ttttgaacga aacctgcaat ttggtttccg cacgtttctg ctgtccgaat ttttcctgta
    17341 cagtcgtacc gcgcgtcggc gaacccgggg catagttggg cgaattgttg taccagagcg
    17401 gcataaggta catcggattg tgttcgcgta cgcccaacag cccgcgcaaa tcgtttttgt
    17461 ccaagtcgta catcaggctc aaaggcgtat agatgtcggc ggtttcgccc gcactgtcgg
    17521 caggaagcgc atccccgcct ttttcaacaa caatgaccgc ctcgccctta tccaggctgc
    17581 tgcggacggt ttccgtcaga ttgagtacgg ctttcgactc ctgcccttcc tgccctgccg
    17641 aagacggaag ctgtgccgca aaaatcctgt cgtaacacgc caaacgcgta acattgtccg
    17701 tcaaagcggc gcattgcagc gcggtctctc caaaagcgga tgccatcggc aacagtcctg
    17761 tcaaaagaat atagcgcata ttccgtgtat tcatctccgc ccccattgtc ggcatattgg
    17821 ttttcaaacg gcattttata gcggattcgg ataaaaaccg caccctttcc gccatttcgg
    17881 gattttgccc ccgcaataca gaaaaccccg aaaccgtcag gcttcagggt tttccgctta
    17941 tcgcgtatca accgccttgg cggttttgca aaaatcaagc cagtgctttt acttttgcag
    18001 acagacggct tttgtggcga gccgctttgt ttttgtggaa cacgcccttg tcggcgatgc
    18061 ggtcgatgac tttgacagac tcttggtaaa ccgcttgtgc ggcagcttta tcgcctgctt
    18121 cgactgcttt caatactttt ttcactgcgg tgcggaatgc ggtacgcagg ctagcgttgt
    18181 gggcgcgttg tttgacggac tggcgggcac gtttgcgtgc ttgtgcgctg tttgccatat
    18241 tggaatctcc tggaagaaaa ataaattctg taaacgcgca attttaaagg gggtttacat
    18301 tatatgcaag ccttttctct ctatccgccc gtcggacacc ctgacagcgc aaaaaaacgg
    18361 caaaatcgtg cggacgtttt ccccctcttt tgaaaacagg gtaatttttt agggaaatca
    18421 gtccaatatc actttgagtt tgcacaaatt tactttacaa tgcccaaacc ttgcgccggg
    18481 gtgtttatgg ggcgcggggt tttctttttt tccaacctct ttcatcagga atctgcccgt
    18541 atgaaaaaat ccgtattagc cgttttggcc gcgctgtctc tggcagcctg cggcggcagc
    18601 gaaaaaaacg ccgtgcaacc tcaagcagac gctgcgtctg ctgccaatgc cgaagccgct
    18661 gccaccgata ctctgaacat ctacaactgg tcgaactatg ttgacgagag tacggtcgaa
    18721 gactttaaaa aagccaacaa tctgaagctg acttacgact tgtacgaaaa caatgaaacg
    18781 ctggaagcca aaatgctgac cggcaaatcc ggttacgatt tggttgtgcc gggcatcgcc
    18841 ttcctgccgc gccaaatcga ggcgggcgcg tatcaaaaag tcaacaaaga cctgattccc
    18901 aactataaaa acatcgatcc cgaactcttg aaaatgctgg aaaccgccga cccgggcaac
    18961 cagtatgccg tcccctattt ctccggcgtg aacacgattg cgattacggc gaagggcaaa
    19021 gagcttttgg gcggcaagct gcccgaaaac ggctgggatt tgctgttcaa acccgaatac
    19081 acccacaagc tgaaatcctg cggcatcgcc ctgtgggaca ccccgagcga aatgttcccg
    19141 attttgctga actacttggg caaagacccc aaaggttcga atcctgaaga tttgaaggcg
    19201 gcggcggaag tattgaagtc tatccgtccg gatgtcaaac gtttcagccc gtccatcatc
    19261 gacgagctgg cgcgcggcga catctgcctg gcggcaggca acggcggcga tttgaacttg
    19321 gcgaaagcac gttccgaaga agtgaaaaac aacgtcggca tcgaagtgct gaatccgaaa
    19381 ggcatgggct tctggattga gtcttggctg attcccgccg atgcgaaaaa cgtcgccaat
    19441 gcccacaaat acatcaacta cacgctcgac cccgaaatcg cggcgaaaaa cggcatcgcc
    19501 gtaacctttg cccccgccag caaaccggcg cgcgaaaaaa tgcctgccga gctggtaaac
    19561 acccgttcca tcttcccgaa cgagcaggat atgaaagacg gtttcgtgat gccgcaaatg
    19621 agcacggatg cgaaaaaact gtctgtcagc ctgtggcaga aaatcaaagt cggcaccaac
    19681 tgatttgaag cattaaaaat gccgtccgaa catcgttcgg acggcatttt atattggatt
    19741 gaaatagaaa tatttatata gtggattaac aaaaatcagg acaaggcgac
//
LOCUS       DAXHNE010000021        19705 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.021, whole genome shotgun sequence.
ACCESSION   DAXHNE010000021 DAXHNE010000000
VERSION     DAXHNE010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19705
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.021"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(338..4507)
                     /locus_tag="ACFM4Z_000587"
     CDS             complement(338..4507)
                     /locus_tag="ACFM4Z_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HFC6020671.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLERFETGKISVGKTFDKQTVYLERLNAAYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIHLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRINPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTLWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLGIANAFGGNMANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRLFGSD
                     KKDSAGNGKGK"
     gene            complement(4562..6409)
                     /locus_tag="ACFM4Z_000588"
     CDS             complement(4562..6409)
                     /locus_tag="ACFM4Z_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HFC6020672.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHVYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQVGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKGGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRASVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAESRKIPGSDIDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGAFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNANVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6819..8048
                     /gene="sstT"
                     /locus_tag="ACFM4Z_000589"
     CDS             6819..8048
                     /gene="sstT"
                     /locus_tag="ACFM4Z_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFC6020673.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALAVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8160..9443
                     /locus_tag="ACFM4Z_000590"
     CDS             8160..9443
                     /locus_tag="ACFM4Z_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6020674.1"
                     /translation="MFERSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPVVAE
                     KETEVKEDAPQAGSQGQGAPSTQGSQDMAAVSAENTGNGGAATTDKPKNEDEGPQNDM
                     LQNSAESANQTGNNQPADSSDSAPASNPAPANGGSNFGRVDLANGVLIDGPSQNITLT
                     HCKGDSCNGDNLLDEEAPSKSEFENLNESERIEKYKKDGKSDKFTNLVATAVQANGTN
                     KYVIIYKDKSASSSFARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGN
                     IFAPEGNYRYLTYGAEKLPGGSYALRVQGEPAKGEMLAGTAVYNGEVLHFHTENGRPY
                     PTRGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGGGDVSGR
                     FYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9518..9733)
                     /locus_tag="ACFM4Z_000591"
     CDS             complement(9518..9733)
                     /locus_tag="ACFM4Z_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020675.1"
                     /translation="MFKLGVYTCLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVFGQYFSEKKMKDDGFIN"
     gene            complement(9726..9941)
                     /locus_tag="ACFM4Z_000592"
     CDS             complement(9726..9941)
                     /locus_tag="ACFM4Z_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFC6020676.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(10016..11359)
                     /gene="argG"
                     /locus_tag="ACFM4Z_000593"
     CDS             complement(10016..11359)
                     /gene="argG"
                     /locus_tag="ACFM4Z_000593"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFC6020677.1"
                     /translation="MSQNHTILQSLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEVSPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEDAAFTPLDRIGQLTMRNLDITDTRVKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            11672..12472
                     /locus_tag="ACFM4Z_000594"
     CDS             11672..12472
                     /locus_tag="ACFM4Z_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFC6020678.1"
                     /translation="MNAFNLIIIGDEILHGSSQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGMPTFVTGGIGATPDDHTRQAAAAALDLPVVRHSEAAKFIEG
                     ITQKRGEPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLETYYADRFNQTERGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            12512..13498
                     /locus_tag="ACFM4Z_000595"
     CDS             12512..13498
                     /locus_tag="ACFM4Z_000595"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFC6020679.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWESDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            14155..14417
                     /locus_tag="ACFM4Z_000596"
                     /pseudo
     CDS             14155..14417
                     /locus_tag="ACFM4Z_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(14455..14829)
                     /locus_tag="ACFM4Z_000597"
     CDS             complement(14455..14829)
                     /locus_tag="ACFM4Z_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6020680.1"
                     /translation="MIIQNEFNLYPSNMLPERFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFRNSILPDLNLIPFASNGEWEAYFDGNDVTGNSRVIVINLDN
                     IENHEFFNSFEDWLELAIKDTW"
     gene            complement(14826..14954)
                     /locus_tag="ACFM4Z_000598"
     CDS             complement(14826..14954)
                     /locus_tag="ACFM4Z_000598"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020681.1"
                     /translation="MPQTWGEAIQFRIKKQIENELAPPNWSTQFPNGSIYDPKVTK"
     gene            complement(15002..15358)
                     /locus_tag="ACFM4Z_000599"
     CDS             complement(15002..15358)
                     /locus_tag="ACFM4Z_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP12 family DNA mimic protein"
                     /protein_id="HFC6020682.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITENQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(15363..15467)
                     /locus_tag="ACFM4Z_000600"
     CDS             complement(15363..15467)
                     /locus_tag="ACFM4Z_000600"
                     /inference="COORDINATES: protein motif:HMM:NF018014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020683.1"
                     /translation="MDDIRGIIQGAVNPLIYKVSKQKIIYNNKLISGT"
     gene            complement(15501..15845)
                     /locus_tag="ACFM4Z_000601"
     CDS             complement(15501..15845)
                     /locus_tag="ACFM4Z_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020684.1"
                     /translation="MNLENYENILIKLLFYHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGVFFNDLLPD
                     NMIINNFFEIND"
     gene            complement(16004..16405)
                     /locus_tag="ACFM4Z_000602"
     CDS             complement(16004..16405)
                     /locus_tag="ACFM4Z_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020685.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINSDKVEVIAVGYRNTDKTIKIFIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDECYAEWILKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIEG"
     gene            complement(16409..16861)
                     /locus_tag="ACFM4Z_000603"
     CDS             complement(16409..16861)
                     /locus_tag="ACFM4Z_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020686.1"
                     /translation="MQTKALGTVDEIGDTVQQVGKQASGQKTSGGNPAIDSDPYSPSS
                     VAARIEAGKARSDLQIKDILSNTTQRSKTKGPAVQYDKVGDYNDALNDFNSLNVRNVQ
                     TRPNGTITGNLPDGRAVNARNDSSGGEPTLEITISNNRKIKIRYGNTR"
     gene            complement(16856..17149)
                     /locus_tag="ACFM4Z_000604"
                     /pseudo
     CDS             complement(16856..17149)
                     /locus_tag="ACFM4Z_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(17219..17536)
                     /locus_tag="ACFM4Z_000605"
     CDS             complement(17219..17536)
                     /locus_tag="ACFM4Z_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020687.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(17533..17793)
                     /locus_tag="ACFM4Z_000606"
     CDS             complement(17533..17793)
                     /locus_tag="ACFM4Z_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC6020688.1"
                     /translation="MRPTTRDELRQALQEQGFRRTGSDAAQYETWKGPDGVKIDIRPN
                     GEVIRTQRVPRTDGVQGKYPQRQDYEGNPLPNNHHHSGYFVK"
     gene            complement(17791..18135)
                     /locus_tag="ACFM4Z_000607"
                     /pseudo
     CDS             complement(17791..18135)
                     /locus_tag="ACFM4Z_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(18561..18905)
                     /locus_tag="ACFM4Z_000608"
     CDS             complement(18561..18905)
                     /locus_tag="ACFM4Z_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020689.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(18943..19287)
                     /locus_tag="ACFM4Z_000609"
     CDS             complement(18943..19287)
                     /locus_tag="ACFM4Z_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020690.1"
                     /translation="MFLDDVNVFLDDLNVFLDDLNTNPITDEWYMSNFADKHIKILES
                     YEAFDILKQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQ
                     EISQDILNEIFR"
     gene            complement(19557..19705)
                     /locus_tag="ACFM4Z_000610"
                     /pseudo
     CDS             complement(19557..19705)
                     /locus_tag="ACFM4Z_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503515.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
BASE COUNT         4622 a         4691 c         5099 g         5293 t
ORIGIN      
        1 gcgggaatga cggaaagtgg cgggaatgac ggttcgggca ttccttaaat tacctgtgta
       61 tcgctgtaaa tcttagagat ggcggaatat agcggattaa caaaaaccgg tacggcgttg
      121 cctcgccttg gctcaaagag aacgattctc taaggtgctc aagcaccgag tgaatcggtt
      181 ccgtactatt tgtactgtct gcggcttcgt cgccttatcc tgatttttgt taattcacta
      241 tatcgattct gttcccttgc aggcggacgg cggcggcgga cggtggaaaa gcaggttttc
      301 aaatgtccgg tttggcggtg cgccgtctga aaaccgttta tttccctttg ccgtttccgg
      361 cggagtcttt tttgtccgaa ccgaataggc ggtcgaaacg tatggtgtat gtcagctcgc
      421 cgcccgacga acggctaccg atacgggcaa ccgcctgtat ggcgcgggtc agccggtaaa
      481 tcagtttgac ggactgttcc gcgctggaga tgctgtattc gtagccgatg tagagtttgc
      541 cggtcagttg tttgccgacg gtcagcacct gttcggcggg gttgagttcg ccggtttgcg
      601 cgttgcggct gcgcttgctg gtaaagccca aatcatccac cagcccgatg cggtcgttga
      661 tttgcccggc aagcagcgcg ccggcggctg cggacagggc ggcattgtcg ccgctgctgc
      721 cgctgccggc gcggttgagg atgagccagg agagcttgtc tttttcactc atcggttcgt
      781 ttgccgtcag cgtaatgcgc ggactgttga ggctgcccaa tatttccacg cccgcaccga
      841 cgggggaaag gcggcgttcg gcgcggatgt tgaggttggg gtcgttgagc gggccgacaa
      901 aggagaccgt gcctttggta atgtccaaat cctgcccgta tgccttataa cgccctttga
      961 tgacgcggac cgtgcccacg ccccgcacgc ttccgcccga ttgggcggtc agggtcagtt
     1021 tgccgcctat ggtaacgtcc gcgccgtagc cggcgaagcg gatgccgtca ttgaggtcta
     1081 aagtcaggtt catattgacg gggagcggtg ccgccgcctc ttttttgact tcgcctaata
     1141 cgacgacatc gtcgccgacg gacggcatcg aggatttttg cgaaccgaac agcccctgat
     1201 ccgttttaat catcccggta acggatatgc ctttttgcgg cgaatagcgc aggcgggtgt
     1261 tgccggaaac cgtcaggcgg cggttggggc gggacaggat gcggtatttg tcgaacaccg
     1321 cgccgatatc gacatcgggt ccgctgtttt ccataccgac cgtaccggag agttccgccg
     1381 tcccttcgtg ccggaatttc aggctgtcga ttacccattt cctgcccgcg atatgcgaac
     1441 gcagcgagcc gttgtccaag atgatgcctt gggtttggtt gcggtaatag agtttgtcgc
     1501 cgttgatgct gccgcccaag tgcggatcgg cgatgctgcc gccgagggtt acggcggcat
     1561 tcaggctgcc tttgacggtt tgtccgaccg gtaggaagtt gcggaatact tcggcatcgg
     1621 caacggtcag gttgagcctg ccgcccaaag gcgcggtatc gaaagagcgg ctttgcccga
     1681 cggtgatgtt gccgttgatt ttcccgtagt tgctgctgcc gttgacggcg gcattgacgg
     1741 acggagagcc tacccgtccg ccgatttgcg cggcggcatt caggctgccg gtaatgtttt
     1801 gcgcggcggc gggcagaaag ggcttcaatg cgcccaagtc gggaagggag gcggtaatcc
     1861 tgccgccgag cggtgcattt gccatattgc cgccgaaggc gttggcgatg cccaaatcgg
     1921 cgttaatccg cccgaaacgc gcgccgccgt caagcaggat tccgatacgg tcgttttgaa
     1981 agcgcgtttt cagggaaaat gcgttcaaac ccaaagcctg cccgccgggc aatacggcat
     2041 cgccgctttg ccggctgata ttgaggtagc cgcgcgcgtt gcgcccgtag gcgacatccc
     2101 agtcgccgtt taaaaccaga ttgtgttcga agggcggttt gaagaaattg tgcaactcgg
     2161 cgatatgcag accgtgtgcg ccgccttttg ccgatatgcc ggttttttta tcccaagaaa
     2221 agtgttgcag gttgaggctg ccgcccattg cctgccaatt tgccgcactt gccgccacgc
     2281 gttccgcacc ggcttcgagc gtcatacggt tttgcagctt gaggttgaat gcgccgccga
     2341 tgtcgagaat gccgatgctg cccttccata gggtaagttc cctgttgatg ccgcctgaag
     2401 cgtccaaatc gaatttgaac ggtttgccat ccagcgtcat ggcggcgtgt gtgcggatgc
     2461 ggtgctgcac gcccgtgccg tccagcatca ggtcggcggt atcgacaacc gccgctccgc
     2521 ccgacagcga aaggcggctg cctttgatgt cggcgcgtat cgggcggctt gtgtcgggcg
     2581 aacctttgag cgtgaaatcg agcgaacgga tgtctgccgc cttgccgatg tgcaggttgc
     2641 gcgccgcgcc ggaaaggtcg gtttcaaagg ttcggatgcc gccgtccaaa tcgccggaaa
     2701 ggtgtccgcg tacatttaaa gaccccgcga gtccgaaacc gaaacgggat aaatcgggtg
     2761 cggtgatgtt gaggttgagc cggtcgcctt ttttgccgaa gccgccgtct gttttaataa
     2821 tgttccgccc cagccgcaaa tcgacggcgg cacgcggaag gtggcgggac tcgtaaacaa
     2881 tgtcggcact gccggcaatc ggtacgccgt tgaacgtgcc gggtaaaaac cgcattttgc
     2941 ctgtgaattt ctcttttgcc agttcgccgg caaggtttat tgagccgttg atattgcctg
     3001 ccggaagttg cggattgatg cgcgaagggt cgaatgcgcg ggaacggatg tccagcttga
     3061 gcaggcggtc tttaaacagc tcgagatagc cttgcgcggt caggctgcct tgcccggcgg
     3121 cgatgttgac ggtgtcgagc accagtttcc gctgtccgtt tgcggggtcg cttgcaatgg
     3181 cgaggctgcc gtccgtgcgc gccgtgccga tgccgagttg ccaagagatt ttgggcgagg
     3241 cggtcgtgcc accgatgccg atgctgccgt ccaacctgcc tttgaacgcg gtttgcagta
     3301 cgtcttccgc gccgacggag ttgatgccta tatttaaatc gaggatgtct ttttcggtgt
     3361 cgattttgcc cgacagcctg atgccgcccc gtccgagcag ggcggcggac gtattgccga
     3421 tatgcaccgt gccgtcctgc cggatgacaa agccgcctaa aacctgacgg acggggatgc
     3481 cgttgcggtc ggcaaagccg gctttggtgt tttccaaatc gagcgaacct tccagcgcga
     3541 tgccgtctga aaacgacggg atggcggtca ggtcgaaatt cagtccggca tcgggcaggg
     3601 aaggcacgaa ggcggccgga ttgatgttga accctttgac cagtacttct tccaatgttt
     3661 tatccaatga ttcggcaaac gggtggatga cggattttcc cgagaggtgg atattgccgc
     3721 cgtcgatcgc cagttcggcg cgcacatcct tcaggctgcc gctcagccga gccgtactgt
     3781 gtatggtttt gccttcgagt ccgcctttgg tgtaaatggc ggtatcgagg gcaaacggtt
     3841 ttttcaagcc gaccgaggct gaccccgacg aactgctcca cggcgtgtcg gcggccttca
     3901 ggtcaaggcg gtgtcctttg cggtcgtaac ggtatgccgc gttgaggcgt tcgagataga
     3961 cggtttgttt gtcaaaagtt ttgccgacgc tgattttgcc cgtctcgaag cgttcgagat
     4021 agacggcggc gggcaggtct atgctgtcgg gaaggctgag cggcgggcgt tcttctttag
     4081 gcggagtcgg tttggtaacg atggcgatgt cgccggcgga aatttcggta atgtgcaggc
     4141 tgcggcgcat cagttcggac ggtttccacg cgaagcggaa gcggctgatt ttaaggtctg
     4201 ccccctcggt ttctatcgac cagttgtcgc cgtcgaagcc gtcgagcagc gtgcctttga
     4261 gattttggga ggaaatgttt acaccgaacc aagacgggat ttggtacagc ccgaagcgca
     4321 aacccgcttc cgtaccggcg agccagccga ggaaacatac tgccaaaatc aggacagaca
     4381 gcagtgccgc cgacagcttc agcagcgggc ggcgtttttt tgccggcggg gtggggcggt
     4441 gttcagacgg cattttgcgc gtgccgtttt cggtcggatc ggtatctgtc ggtgcggtat
     4501 cggtcataat gtctgtttca aaatagtttg ctgcaatgcc gtctgaagcg gccgtatcgg
     4561 tttagaagcg tgttcccaag ctgatgtgcc agcggatttt cttatcgctg tgcccgtagg
     4621 cgatgtcgaa ggaaaacggc gcgagcgggc tgaaccagcg cacgcccagt cccgaaccgt
     4681 gtttcagctt catacgtttg aaattggcgg cggcatcgcc catatcgtgg aataccgcgc
     4741 cggaaagggt gcgcgtaaac ggcagttggt attccaggct gcccaccaag agggcgcgtt
     4801 cgggcaggac cgatccgttc gggccggcaa gcccgatgct gtcgagttcg taaccgcgca
     4861 cggaagacgc gccgccgctg cggaacatca gccctgaagg aacgttggca ttgtcgcggg
     4921 caacggtgta acccgcttgt ccgcgtatga tgaacgtgcc gagttttttg ttttcgggcg
     4981 tgaagaaata acctgcgcgg gcagaggtgc ggatcagcgc ggtggaggac aggaatgcgc
     5041 ccaaagtcgt accgattttg ccgtcgaggt aatggccgtt ttcgggatgc agcacgttgt
     5101 tgagcagctg gcgtttccaa gaggcggtca gcatcgtggc gtggctgttg cccaaatcga
     5161 tatccgagcc ggggattttc cggctttctg cgagaaactc cgcccccagc ctggcatcga
     5221 tgcccgcgcg gtcgcgcaca taccagatgc cgccggagaa ggcgcgtttt tcgaggtttt
     5281 gggtggtcga acggttgtag gaaacgttgc ttgtccagta gttgccccga tagttgcgcg
     5341 gctggctgat gccggcggca agcgtggttt cgtatttgtc catatcccaa acgaccgagc
     5401 cgatatagcc tttgttgaag agattgtaat agtcgtaggc gattttgccg cccaaaccgt
     5461 attccgaatc gaggcggatg ccggtttcga gtttgtggcg tttgacctcg gttacgctga
     5521 ctttgacggg gacgcggtcg ccttggaggc ggtcgaagtc ggcttgtacg gacgcgccgg
     5581 aataatgccc gttttgttcg agcgcctgtt ggaagtcgag cagcaggtcg aggtcgtagg
     5641 gcgtgcccgg ttggaagcgc gccaggccgg agacgatttg ttcggggtaa cgctgtgtgc
     5701 cggtgatttc aaagtcgccg aaggcgatgg ggcggccgct gtccacgacg acgttcaaat
     5761 cggcggtggc ggtatcgggg ttgacggacg cccgggtgtt gccgagcttg gcaagcgggt
     5821 agcctttgcg cgttaccgcg ccgaggacgg aagttttgct gttttcccaa ctgtcctgat
     5881 cgaaatcact gcctaccggc tgctgccagt tttccagcgc gttgcggtag tattcggcga
     5941 ggttgccgtc tgaaaggatg tcgccgagga tggcgacacc gacgttggcg attttggtgc
     6001 gcggccccgg tgtgatgtgc accgtataag ccccgccttt ttccgtcagg ctgactttgc
     6061 tgctgaaata gcctttgctg cggagcatgg ttttgacgtt gtccggtgct tcttcggcaa
     6121 ggaagcccac ctgttcctta tccaaaacct cttcctgctg ctgcgtgatg agcggcaggt
     6181 gttcttcgac catatctttg atttcactgt cctgcgtgtc gatgcggacg gggaatttgg
     6241 gttttaattt gactgattcg gtgtcggggc ttttgttttt gaacaatgcg aaacccgccg
     6301 ccttgttttc ggaaaggtcg gcggcaggcg cgtatacgtg cggaaagaaa aataaagccg
     6361 gcaggagcag ggcggtcggt ttgatcatca tggtacgggt gtcgtgcatc ggtgtggcgg
     6421 ctttcgattt taacactgtt cggacggggt ttcgaacggc tgtttttgtg ttgttcggac
     6481 ggggcgggac acttcagacg gcatactgcc gccggtattt cggaggttgc ggcccgccgg
     6541 aaatgtatct gcttgtttta aggtattttg tttccaatcc gcatatggaa aacaggctgc
     6601 gtttcggaat agtgccggaa aataggcagg attaaataaa tttattttcg ttcaataaat
     6661 taaaccgttg cgccgaatga taaagcaaag cctgtccggc actgcatagg gcaaatcaac
     6721 gcaaccttaa aacacagccg aaaaataaaa aaacacagag aatgagtttt cttttaaaat
     6781 acaacatttt ttaacatatc acaacataag gaagaattat ggcgttcggc aaatccttat
     6841 ttcatgcgat cggccgcgtc agcctggtca ggcagattgc cgccggtttg gcgttgggca
     6901 tcgtaatcgg ttcggtttcc ccgcaactgg gcttggcggc aggcttgttc ggcagcctgt
     6961 ttgtcggcgc gctcaaagcg gtcgcgccgg ttttggtatt tattttggtg gcggccacaa
     7021 tcgcgcagca ccaaaaaggc aacaaggcgc atatcaggcc gattatcgtc ctttacctca
     7081 tcggtacgtt ttccgcagcc ctgaccgccg tcatcgccgg tatggttttc ccgacgcaca
     7141 ttgttttggc gggcgcgggc gatgtgtccg ccgcgccgcc ttccggcatt gtggaagtat
     7201 tgaaatcgct gctgatgaac ctggtcgcca acccgattaa cgcgattgcc aatgccaact
     7261 atatcggcat tttggcttgg gctttggttt tgggcgcggc gttgcggaat cacggttcgg
     7321 acgttacgcg gcaggtcgtt gccgatttgg cggaagcggt ttccaccgtc gtgaaatgga
     7381 ttatccgttt tgcccctttg ggcattttcg ggctggtgtc gtccacaatc gcggagacgg
     7441 gtttcggcgc gctggcgggg tatgcgaagc tgcttgcggt gctgttgggc tgtatggcgt
     7501 ttatcgcgct ggcggtcaat cccgccatcg tgtggtggaa aatccgccgc aacccttatc
     7561 cgctggtgtt tacctgcctg cgcgaaagcg gcgtgtatgc cttctttacc cgttcttccg
     7621 ccgccaatat ccccgtgaat atggctttgg cgaaaaaact ggggctgcac gaagacactt
     7681 attcgatttc catcccgttg ggggcgaccg tcaatatggg cggcgcggcg attacgatta
     7741 ccgttttggc tatggcggcg gcgcacaccc aaggcataca ggttgatttt gccaccgcgc
     7801 tgctgttgag cctagtggcg acggtcagcg cgtgcggcgc gtccggcgtg gcgggcggtt
     7861 cgctgctgct gattccgctg gcgtgcagcc tgttcggcat cagcaacgat gtcgccatgc
     7921 aggtggttgc cgtcggcttt atcatcggcg tgattcagga ttcggcggaa acggcgttga
     7981 attcttcaac cgatgttttg tttaccgctg ccgccgattt gggccgacag agaaaccggg
     8041 cggaataaac caagctatgc cgtctgaagc ccgtttggcg ttcagacggc atatttttac
     8101 aattttatgg gcatatggcg gaaacaatac cggctgcctt gaatatgaag gggatgatga
     8161 tgtttgaacg cagtgtgatt gcaatggctt gtatttttgc cctttcagcc tgtgggggcg
     8221 gcggtggcgg atcgcccgat gttaaatcgg cggacacgct gtcaaaaccg gccgctcctg
     8281 ttgttgctga aaaagagaca gaggtaaaag aagatgcgcc acaggcaggt tctcaaggac
     8341 agggtgcgcc atccacacaa ggcagccaag atatggcggc agtttcggca gaaaatacag
     8401 gcaatggcgg tgcggcaaca acggacaaac ccaaaaatga agacgaggga ccgcaaaatg
     8461 atatgctgca aaattccgcc gaatccgcaa atcaaacagg gaacaaccaa cccgccgatt
     8521 cttcagattc cgcccccgcg tcaaaccctg cacctgcgaa tggcggtagc aattttggaa
     8581 gggttgattt ggctaatggc gttttgattg atgggccgtc gcaaaatata acgttgaccc
     8641 actgtaaagg cgattcttgt aatggtgata atttattgga tgaagaagca ccgtcaaaat
     8701 cagaatttga aaatttaaat gagtctgaac gaattgagaa atataagaaa gatgggaaaa
     8761 gcgataaatt tactaatttg gttgcgacag cagttcaagc taatggaact aacaaatatg
     8821 tcatcattta taaagacaag tccgcttcat cttcatttgc gcgattcagg cgttctgcac
     8881 ggtcgaggag gtcgcttcct gccgagatgc cgctaatccc cgtcaatcag gcggatacgc
     8941 tgattgtcga tggggaagcg gtcagcctga cggggcattc cggcaatatc ttcgcgcccg
     9001 aagggaatta ccggtatctg acttacgggg cggaaaaatt gcccggcgga tcgtatgccc
     9061 tccgtgtgca aggcgaaccg gcaaaaggcg aaatgcttgc tggcacggcc gtgtacaacg
     9121 gcgaagtgct gcattttcat acggaaaacg gccgtccgta cccgactaga ggcaggtttg
     9181 ccgcaaaagt cgatttcggc agcaaatctg tggacggcat tatcgacagc ggcgatgatt
     9241 tgcatatggg tacgcaaaaa ttcaaagccg ccatcgatgg aaacggcttt aaggggactt
     9301 ggacggaaaa tggcggcggg gatgtttccg gaaggtttta cggcccggcc ggcgaggaag
     9361 tggcggggaa atacagctat cgcccgacag atgcggaaaa gggcggattc ggcgtgtttg
     9421 ccggcaaaaa agagcaggat tgatgctgat gccgcctgaa gcgggggcgt tcagacggca
     9481 ttgtgatgtt tggggtgggg tccggttcaa atccgcatca gttgataaac ccgtcgtctt
     9541 tcattttctt ttcggaaaaa tactggccga ataccagcag ggcggcgagg atgatgacaa
     9601 acagccccgc catagtcagt gtgattttga agaacaattc cgcttcgaga aaagaaaacc
     9661 agagttgcac gatcagcagc agcacccagc cggcaaacag tccgagacag gtataaacgc
     9721 cgagcttaaa cacgatcccg ctccaaaatg acgacgtagg cctcgcgttt gaaaaacagc
     9781 agcatacggc gcaaatcttt ttccatcgtt acaacccgcc agccttcggg ggtttgtttg
     9841 ttgaggaacg cgctgaaatt gacggggttg acctttgccg cgccgaaaaa caggctggac
     9901 aacaggcttt cctgataaat gacgactttg tattctttca taggattcct tttaacataa
     9961 gggatcatct tttcagacga ccccttaagt tgctaatgat gtaaaacagc aaaccttatt
    10021 gcttattgcc caactgcggc aataccgaac cttcgcccag cgagagcaaa ccgctttgcg
    10081 agtagatacc cagtttgacg cgggtgtcgg tgatgtcgag gttgcgcatc gtgagctgtc
    10141 cgatgcggtc gagcggagtg aacgcagcgt cttcgacttt ttccatactc aggcgttcag
    10201 gttggtaggt caggttgggc gattcggtgt tcagaattga gtagtcgttg ccgcgccgca
    10261 gttcgagggt aacttcgccg gtaacggctt tggcaaccca gcgttgtgcg gtttcgcgca
    10321 acatcagggc ttggctgtcg aaccagcggc cttggtagag caggcggccg aggcgcaggc
    10381 cgttgatgcg gtattgttcg atggtgtctt cgttgtggat gccggtgacc aaacgctcgt
    10441 aggcgatgtg gaacaacgcc atacccgggg cttcgtagat gccgcgcgat ttggcttcga
    10501 tgatgcggtt ttcgatttgg tcgctcatac ccaagccgtg gcggccgccg atgcggttgg
    10561 cttcgaggaa gagttcgacg ggatcggcgt attctttgcc gtttagtgca accggcacgc
    10621 cttcttcaaa gcgtacgctg acttcttccg ggctgacttc gacgttttcg tcccaaaagg
    10681 caacgcccat aatgggtttg acgattttga tgcccgagtt caaaaattcc aagtctttgg
    10741 cttcgtgggt ggcacccaac atattggaat cggtggagta ggctttttcc accgacattt
    10801 tgtagttgaa gccgttggca atcagaaatt cgctcatttc gtgacggccg ccgagttcgt
    10861 cgataaattg ctgatcgagc cagggtttgt agattttcag cgcgggattg gtgagcaaac
    10921 cgtagcggta gaaacgctcg atgtcgttgc ctttgtaggt gctgccgtcg ccccaaatat
    10981 tcacatcgtc ttctttcatt gcggaaacaa gcatagtgcc ggttacggcg cggcccagag
    11041 gcgtggtgtt gaaataggcg atgccgccgg tggaaacgtg aaacgcgccg cattggatgg
    11101 cggcgatgcc ttcgtgtgcc aactgcgcgc ggcagtcgat taagcgggcg ttttccgcac
    11161 cgtattccat cgcttttttg ggaatggcgt tgtagtcgtc ttcgtcgggc tggccgaggt
    11221 tggcagtgta ggcataaggc agcgcgcctt tgagtttcat ccacaacagc gcggcagagg
    11281 tatcaagacc gccggagaag gcgatgccga ctttctgacc gacggggagg gattgcagaa
    11341 tggtatggtt ttggctcata agggtatcct tcggacttga aaaaaggagt tcattatctt
    11401 ataaatataa cccattgtca gccgttaagg ctgtctgaaa agaagctttg cccgtttcgc
    11461 tgaaaatctt ttcagacggt ctttacgcca tcctcaacct gactccctcc cctgcggggg
    11521 agggttgggg agagggtaca gacagcaggc cttaagcaaa cgctgcctga aaaacgcaag
    11581 ctcgaccctc tccctaaccc tctcccacgg gagagggaac aaatcacagc accatcgaca
    11641 ttttcagacg gcatcggtaa agaaaccatc tatgaacgcg ttcaacctca tcatcatcgg
    11701 cgacgaaatc ttacacggca gcagccaaga caagcatttc gcctttttca aatccctgct
    11761 cgaatccaaa gggctgaagc tcaatcaggt gcaatacctg cccgacgaac ccgatttgct
    11821 ggtcaggcaa ctgcgccgca gcttttcaga cggcatgcca actttcgtta ccggcggtat
    11881 cggcgccacg cccgacgacc acacccgcca agccgcagcc gctgctttgg atttgcccgt
    11941 cgtccgccat tctgaagccg ccaagtttat cgaaggcata acccagaaac gcggcgagcc
    12001 gcttgattcg ccggaacacg cccaacgcct gaaaatggca gattttcccg aaggcgcgga
    12061 attggtgccc aatccgttta acaacatcgc cggattttct atccgcgagc attatttctt
    12121 ccccggcttt cctgtgatgg cgcacccgat ggcggaatgg gtattggaaa cttattacgc
    12181 cgaccgcttc aaccaaaccg aacgcggcag ccgcagcgtg tatgtgtttg agcagcccga
    12241 atcgcgcatt acgccgctta tggagcatat cgagcaaact tatcccggcg tgcgttcgta
    12301 cagcctgccc agcgtcggtt ggacgcattc agacggcacg caggtcaaac cgcacattga
    12361 gttcggcatc aaagccgaag gagaagccgt caaccttttg gacgcggcgt gggcggaagt
    12421 gttgcacagc ttggacggat tgggagcaga gctgaaaaat cgggtaaact gaaaaccgtt
    12481 tgcccaaatc ggacaatatt taaggaaaac catgcaatac cgaatcagaa gagaaaacga
    12541 agcacccgaa gcaaaaaatg cgggggaaac cttatgggag agcgacatca tgcgcgaagt
    12601 gctgttgtcc gcctatcagg acaggcgcag ggagcggatg tggaaaaaca tctggcgcgc
    12661 cgtcagcacc ctgattctgg ttgccctgat tgcaggcatt ttccgaaaag acgaagccgc
    12721 attgcagttg gcgggcaaca cgccgcatac cgcagtcgtc aatctgtacg gcgaaatcgg
    12781 caacggtgta gaagaccagg tcaaaaaact caaagacggt atggaggccg cctacaaaaa
    12841 cccgcaggca aaagccatcg tcatccgcgc caacagcccc ggcggttcgc ccgtcgtgtc
    12901 caacaccgct tttgaagaaa tacgccgtct gaaggcgcag caccccggca ttcccgttta
    12961 tctcgtggcg gaagatatgt gcgcgtccgg ctgctactac atcgcggcgg cggcggacaa
    13021 aatctatgcc gacccgtcca gcatcgtcgg cagcatcggc gttatcggca gcagtttcga
    13081 cgcgaccggc ctgatggaaa aaatcggcgt gaaacgcagg gttaaaattg caggcagcaa
    13141 caaaggcatg ggcgatccgt tttcgcccga aacgcccgaa cagtcaaaaa tctgggaaga
    13201 aatgctgacc ggcatacacg gcgagttcat caaagccgtc aaaaccggac gcggcggccg
    13261 cctcaaattc cgacaatatc ccgacgtgtt cagcggtcgc gtgtacacag gcgcggatgc
    13321 gctgaaagtc gggctggtgg acggactcgg caatatttac agcgtggcgc gagacgtggt
    13381 caaagccccc gatgtggtgg attacactcc gaaggacgat ttcggcagaa tcttgggcag
    13441 acgcttcggc gcagaactca aagcaagcgt gcgcgaagcc ctgcaggccg tccggtagcg
    13501 ttgtttacag tatttctaag ggaataccaa aataaaaata tacacaaaaa cactcacctc
    13561 cccagtatgc tgccactaag tcagccgcgt cactatttac cgtgcactta aagccgtaaa
    13621 aggcaggttg tccccgggag gttttgtgca tcctgatacc gatcggttgt tgttgctcaa
    13681 aggacagaag gccgctgata aacgagatta cctgtttgtc gctattgacg atttttatac
    13741 tctgccattt tgccggacaa aaccgcagac agtgctgcca agtttctgac cgaacatctg
    13801 tccgacccct gcttgtacct gattgagtac gtttactctg acaatgatag gtaatataaa
    13861 gagccgtcca acatgctttc ggtgcagctt gttatgataa tgggattggt tggaggcttg
    13921 cccgatttgc ttgtccgcag accaacggta aggcggagcg ggttatccgt accttgatgg
    13981 agatgtggca tgaggaacag tcgtttgaca gtccggaaca tcggcaaaag gagttgtgct
    14041 gctttattaa tttaggggct gccctagatc actagggaaa ttcaaattaa gttagaatta
    14101 tccctatgag agaaagtcgt ctaagacggt ataaacaaaa taaactcatt ttgttatcgt
    14161 ggctatgatg tattggatgc gggcgaattt agccatttcc gtatcaatca cagcacacat
    14221 tttgccgaac gacaaaacca tattaatgga actgagaact tttggaatca agcaaaacgt
    14281 catttacgca agtttaacgg cattcccaaa gcgcattttg agctgtattt aaaggagtgc
    14341 gaatggcgtt ttaacaacag tgaaataaaa gttcttgttc cattttaaaa caattagtaa
    14401 aatcgagttt atcctagttg tccagggcag cctcatctct ttatagatag ctgcttacca
    14461 agtatcctta attgctaatt caagccaatc ttcaaaacta ttaaaaaatt catggttttc
    14521 tatattatct aaattaatga caataaccct agaatttcct gttacatcat taccatcaaa
    14581 ataagcttcc cattctccat tactagcgaa tgggataaga tttaaatcag gcaggataga
    14641 atttctaaat atataagcta cttctgcccc ttctgttcca taattctcaa accaccagtg
    14701 aaaattatgt ggctgaatat aaggtattaa agatgtatcg ttagagatac gaacatactt
    14761 ttcaggataa caaaaccttt caggaagcat attactagga tataaattaa attcattttg
    14821 aataatcatt tcgttacctt aggatcataa atactaccat taggaaactg ggtagaccaa
    14881 tttggtggtg ctagttcatt ttcaatttgt tttttaattc taaattgaat agcttctccc
    14941 catgtttgcg gcatctaatg ctgcttgctc tgcatcaata ttactaaaaa ttggattagt
    15001 ctcaaaaatg aaaaattact gttttttcat tttttatagc atattcaagt acatgaataa
    15061 tatttttcac taagggctct gtagaaaaat ctgaattttg taatttttct tttacagcca
    15121 aaatactttc cttatctatt agccaatccc cctcacttgg tccaaataac caattatcgg
    15181 taatttcaga aacaagtctc cataatccac gattttgata ttcaataatt tgattttccg
    15241 taatttctac aactgtttca gattgaatgt ttttataagc ccaatcaatc atttcagtat
    15301 attcactaat tgaaacattg tcttttaaac agaaaattaa caggttgtgt tcattcattt
    15361 gtctaagttc ctgatatgag tttgttgttg taaataatct tttgcttgct taccttgtaa
    15421 attaaaggat taaccgcacc ttggatgatg ccgcgaatat catccatacg gttaccccac
    15481 caatttttat tggtgtatcg ttaatcattt atttcaaaaa agttatttat tatcatatta
    15541 tcagggagca ggtcgttaaa aaaaaccccc tcaaattcca ttaaattagt atttatccag
    15601 cacataaacg aaccaaagcg ttctttccct gagtgtaaat atttaaaatt aaactcaaaa
    15661 taagtttttt tgcttagaaa atttaatggt ttttctatag tgaaaaaaaa gcccttactc
    15721 gttttcgttc gagatactat tttaaataaa tttttatttt caataaaata agaatattca
    15781 tttactaagt tgttatgata aaaaagtaat tttattaaaa tgttttcata attttctagg
    15841 ttcatttcta gctcaattta aaatgatttc attgttgccg taccaatgcg aaatttacca
    15901 tcaacaaaat ttcccttcac tgatacaggt acaccatcaa cttgggtagt tttattttca
    15961 ggctgcctga aagaaagata ggcaacttat tactcatctt acgctagccc tcaataattg
    16021 aatacgttgc actagaacca attatttcta tgaatgtttg ctcaaacata aaaatatagt
    16081 atttaaagaa ttctctattc aaaggaaaat aactttcttt caatatccat tccgcataac
    16141 actcatcaag aagttcaaaa aaagattgac caccattttc atgaagcaaa gctctatctg
    16201 catcttccga aattaaatcc ataaaagtat ctataaaata cgattcatca acaaccctaa
    16261 aatgaataac atcttcaata aaaattttta tggttttatc cgtatttcta taaccaactg
    16321 ctataacttc tactttatca gagtttattg ctgcattatc aaaatagaaa tagccagttg
    16381 ggaaatctaa gctttttaat ttcataattt atcgtgtatt tccatatctg atttttattt
    16441 ttcggttatt actaattgtt atttcaagtg ttggttctcc accactacta tcattacgag
    16501 cattaaccgc acgcccatca ggtaaattgc ccgttatcgt tccattagga cgtgtttgta
    16561 catttcgaac attcagacta ttaaaatcat ttagtgcgtc attgtaatcc cccactttat
    16621 catactgaac agcgggacct tttgttttac tcctttgagt agtattgctc aaaatgtctt
    16681 tgatttgtaa atcactgcgc gccttaccgg cttctatgcg agctgccaca ctactcgggc
    16741 tataggggtc gctatcaatc gcaggattac caccgctggt tttttgtccg ctagcctgtt
    16801 tcccaacctg ctgtactgta tcgccaattt catctaccgt acctaacgcc ttagtctgca
    16861 tatggcgaac agcaggtttt ttatagccgg tatttttaac ccaatcccat ttggtcgggt
    16921 taagttctac ttttttacct ctccaaaccg tacctgcggc ctctgctacg gcaagggcag
    16981 tttgggctgt tgctgttata ttcggatggg catcagccca ttgtctggcg gaattgatgc
    17041 cgtcttttac cgcaaaagca ctgtcctgta atatgctcgc ggcagcaagt tgtgcttccg
    17101 ggcttaattt tcctgaatta ttaatacctt gtagagtctg ctgcgcagag atgtagcaac
    17161 tgtattttta ccccgtcggg taaaaataca gttgctacgg ctgctgggaa agcagttact
    17221 attttcccag tggctcaaaa ttaattgttt cagcagatat ttttataata atccctacat
    17281 caataataat ttcactataa aattggtatt tatatagctc tatttcgggt ataaacaagg
    17341 gaaacaagct atcttcctta tcctcataat ctacgaaatt atttgtatct aatttaaaat
    17401 tcaaaatatt actgcatttg atatatccga aataattacc ttcaattgta tctgtaaaag
    17461 ataatgtaag ggtatttcca ttatcggaaa agattatttc ataaaggctt acattgtgaa
    17521 aaatattttt tttcatttga caaaatatcc agaatgatga tgattatttg gcaatggatt
    17581 gccttcataa tcttgtcgtt gcggatattt tccctgtaca ccatcggttc gcggcactct
    17641 ttgggttctt ataacctctc catttggacg aatatctatt ttcacgccat caggaccttt
    17701 ccatgtttca tattgagccg catctgaacc agtacgtcta aaaccttgtt cttgcaatgc
    17761 ttgacgtaat tcatctcgcg tagttggtct gatagattta acaactggct tgtttcctcc
    17821 tgccatctca ccgtccaacg tctgcatagg gcgggcagca ggtgttttat agccgttatt
    17881 tttaacccaa tcctatttgg tcgggttaag ttctactttt ttacctctcc aaaccgtacc
    17941 tgcggcctct gctacggcaa gggcagtttg ggctgttgca gttatattcg gatgggcatc
    18001 agcccattgt ctggcggaat tgataccgtc ttttaccgca aaagtactgt cctataatgc
    18061 gcttgcggca gcaagttgtg ctttcggact taaatttcct aaatcattaa taccttgtaa
    18121 agtttgctgc gcagagatgt aggtcggata tttgtatccg acaaaaatat ttgacgcatc
    18181 tatcgtttcc gaaatgccgc tgttggaaaa tgtcggattc gagaatccga cctacggcta
    18241 tttcgtttaa gtcataaata gccattcatg ttccttctga atttgctttt gctatcctta
    18301 gaaccaatca tataaacatt atcatcatta attaatgttc taagtttatg aagcggtcaa
    18361 atctgtcaaa ctaccagaga tgaagcatat cctcctggaa gtaattctgt tcgtgctgtt
    18421 tccatatctt ctttgccatc atcagttact tctctgtcta aataaaatct tacgataagt
    18481 ttttgtctaa tttcatcaaa ttctgccgta attaatctta tattgatatg aatacacggt
    18541 tcatacctgc tattagccag tcaggtgtag ttggatgggg attcattttt aataatatct
    18601 tcaagcaagt gaaatgtaag aaaagcgtag atacttgaag gccggctatc tgattggcaa
    18661 aaagaaattt cggagataaa gaatttccgt ttatattttt cataaattaa atcaacacct
    18721 tgttttacat aaaaactaac ttcctcagga aggatattta taacattctc tccttttact
    18781 tttatagtta atggtttgat ttttatgttt tcatgaatat ctgataggat tatttctaaa
    18841 aaattatttt tatttccagt cattctggct attttatgta tatttccatt gtgttcgatt
    18901 atcattttat tttttccaag gatttccttc tatattgata gtttatctaa agatttcatt
    18961 taaaatatcc tgactaattt cttgtttata taattctaca attttctgtt tctgtgtatt
    19021 tgtataaaat ttttcgttgg tatctgcttg atattttaat tgtctcaata tttccatgat
    19081 ttcatattct gatttttcat catattcttc aatcatgtaa tcaacaaatt gttttagaat
    19141 atcaaaggct tcgtaacttt ccaaaatttt aatatgttta tcggcaaaat tggacatata
    19201 ccattcgtca gtgattggat tagtatttaa atcatctaaa aaaacattta aatcatctaa
    19261 aaaaacattt acatcatcta aaaacatgac taatccttaa acttaaaatc tgttttctca
    19321 ccggttcttg agttttcaat gtaatgtatt tcaattttgt ttacgttttg aacacgctta
    19381 acccaaccgt cttttgctaa ccaaccattt ttagtatcgg acataggagg tattctcgca
    19441 ggagttttgc cctgtgggtt tgccttaact tcttccattg ctaattgttc ctttaaatta
    19501 ttaggtattg tacgaccggt tgatttttca acagattgtg tccctatttt taatgatttg
    19561 tttcccccag ccatttcccc gtctacagtc tgcatagggc gggcagcagg ttttttatag
    19621 ccggtatttt taacccaatc ccatttagtc gggttaagtt ctactttttt accgcgccaa
    19681 accgtacctg cggcctctgc tacgg
//
LOCUS       DAXHNE010000022        18374 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.022, whole genome shotgun sequence.
ACCESSION   DAXHNE010000022 DAXHNE010000000
VERSION     DAXHNE010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18374
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.022"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            142..1225
                     /locus_tag="ACFM4Z_000611"
                     /pseudo
     CDS             142..1225
                     /locus_tag="ACFM4Z_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519957.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
     gene            complement(1395..1604)
                     /locus_tag="ACFM4Z_000612"
     CDS             complement(1395..1604)
                     /locus_tag="ACFM4Z_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020691.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPAYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(1601..1897)
                     /locus_tag="ACFM4Z_000613"
     CDS             complement(1601..1897)
                     /locus_tag="ACFM4Z_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HFC6020692.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRRARL
                     AQKPKTRWKILYTLINGVFFDRHHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            complement(1876..2481)
                     /locus_tag="ACFM4Z_000614"
     CDS             complement(1876..2481)
                     /locus_tag="ACFM4Z_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020693.1"
                     /translation="MTIYFKNGFYDDTLGGIPEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSDYHEWDGKKWKISKAAAAARFAKQKTALAFRLAEKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVIEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            2561..2848
                     /locus_tag="ACFM4Z_000615"
     CDS             2561..2848
                     /locus_tag="ACFM4Z_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HFC6020694.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVRFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            2820..3122
                     /locus_tag="ACFM4Z_000616"
     CDS             2820..3122
                     /locus_tag="ACFM4Z_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HFC6020695.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYIAQLK"
     gene            complement(4121..6094)
                     /locus_tag="ACFM4Z_000617"
     CDS             complement(4121..6094)
                     /locus_tag="ACFM4Z_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin knob domain-containing protein"
                     /protein_id="HFC6020696.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVITGDQTVNGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHGANNRISYNNHDIFHKANKPRFAEDIQGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAVGSSNLPVENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVQNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNSLAVGTKQEDEIKRHVHKVFSHWTNHTDAA
                     ALGYEDRNERQRSALVSTWTDENLNDNGFLTPRSDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQGLQDKADRDHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSERVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRRDGLLEALQD"
     gene            complement(6105..6664)
                     /locus_tag="ACFM4Z_000618"
                     /pseudo
     CDS             complement(6105..6664)
                     /locus_tag="ACFM4Z_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
     gene            complement(6661..7715)
                     /locus_tag="ACFM4Z_000619"
                     /pseudo
     CDS             complement(6661..7715)
                     /locus_tag="ACFM4Z_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
     gene            complement(7729..8076)
                     /locus_tag="ACFM4Z_000620"
     CDS             complement(7729..8076)
                     /locus_tag="ACFM4Z_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFC6020697.1"
                     /translation="MDKELNPGTGDYTGRTVDTLQNAVYIRLMTPLGSWWADKTLGSL
                     LHLLQREKDLQRVSLLAEQYADEALQPIVKSGRADKITVRAEQPHDGRLILHIRVDTA
                     AGGFDYRHEVPVI"
     gene            complement(8177..8845)
                     /locus_tag="ACFM4Z_000621"
     CDS             complement(8177..8845)
                     /locus_tag="ACFM4Z_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFC6020698.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRAAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDLEHLQEYGFASHPPDGSEAVVIPLGGNTSHGVIVCSQHGSYRIKNLKPG
                     ETAIFNHEGAKIVIKQGKIIEADCDVYRVNCKQYEVNAATDAKFNAPLVETSAVLTAQ
                     GQINGNGGMAVEGGDGATFSGDVNQTGGSFNTDGDVVAGNISLRQHPHTDSIGGKTLP
                     AEPA"
     gene            complement(8842..9986)
                     /locus_tag="ACFM4Z_000622"
                     /pseudo
     CDS             complement(8842..9986)
                     /locus_tag="ACFM4Z_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
     gene            complement(9976..11307)
                     /locus_tag="ACFM4Z_000623"
     CDS             complement(9976..11307)
                     /locus_tag="ACFM4Z_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HFC6020699.1"
                     /translation="MSGWHTLLQDASYKGVGFDIEVVDESNGKALAEHARPFVQGIDL
                     EDMGTTGRQVQINAVFWGKGYAGRLKKLLDALEQPGGGVLVHPVWGRMHNMIAASWSY
                     RHEADYVDYAGIDITFREAAEAQEIFVFENAFLVELEALIADIDTYREAAIGFVDAVL
                     AVDAGVSALWGSALGIWSAASGTFGAVRRLFDLDKIAFPDRGGYSAAAFKNGSAKLFA
                     DISVMVDTGIRREAGLADNAMHHAGWSPRQRFDGAAAVADRAAAIPDNLLTGRFSDGL
                     QNRLNRLTAKQVQPVAQAVRLLSTSSLLSVATALIEAHGEEMTAPDLIEVNRAMRRRM
                     QAEIAALRAVQTAAAESGGLTANAVYTEAYQTAESLRAAAGRLNALVAAVINQKPPLI
                     VRQAPIDGTIHQIAHEFYGDIARAAELVRLNPHIHHPAFIKRGTLVNSYAK"
     gene            complement(11307..13478)
                     /locus_tag="ACFM4Z_000624"
     CDS             complement(11307..13478)
                     /locus_tag="ACFM4Z_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein F"
                     /protein_id="HFC6020700.1"
                     /translation="MANGNMKLSLVLTARDDGARRLLADTQRQLDRTAKSRAQLERQS
                     HTYALTGIRSEKQIQREIMLTQAAFNRLARSGKASQNDLARAAVATRNRIRELNAELK
                     QGTGFADKMGKIGRFGAAAVAGGAAAYTVLKPAMDNRKQLDENINRVSRQAFIEDNSK
                     SAAWIATEGAQQIKDLALELVEKNGGTHDKALDLISGMMTTSLNFAQTKNEAQAAYAF
                     ALASEGSGEDTAKLIKTLKDGGMSGKDLQLGLEHVLQSGLDGTFEVRDMVRELPSLLP
                     AAQQAGMNGVGGLDYLLSLLQSAANKSGSPAEAATNVQNLLSKTLSPDTIGRLKKMAN
                     PNDPKKGVDWIGSVVQGKQNGENAVQVLSRLADAMLVKDKQYQDYKKRAAAGDKTAAE
                     QANMLKGALLAQLLPDLQAKQGLLAATDMTQIREYMASLAGVTLDNGKIAKNNEARML
                     SAAAQQEQQESLAMLRESLTGTLVDMETSFKKLAAEYPNATLALQALTTAATAASAAM
                     LLTAGGGKGAGFLKDVGSKALGWGKASAGGVAAGATAAGGKLLSWGKSAGSGLMNNPA
                     LVKRAGLLGMLLYSESLGDGTLPKGLRGTKTTPEMINRLKNNGIRFEPAPKREQARGG
                     VPQYLAAPSAQPTDKMLSPLFSTQTAAYQAAIQQQTAAYQAALAQDTAAVTTGLAQVQ
                     SAMASASQTINTNVSLNIDGRVIANEVSRYQVAMFGRGAGQ"
     gene            13633..14235
                     /locus_tag="ACFM4Z_000625"
     CDS             13633..14235
                     /locus_tag="ACFM4Z_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020701.1"
                     /translation="MNMKKLISAICVSIVLSACNQQSKTAQAEEPVQSIQAADCTAPM
                     DITVEQYLINLEQAFKTQNVSTKIHNKNIVKTDCGYDLTLVMDFGAIALKLDEQQKIR
                     AISVGYILKTDGEKGQNLVNNAINGLHSIQAVLSLTTTDKLGESEAGKQLFTALTEVV
                     KESNQTGATAQKDVPADGILYSVVFEKETNTIAIIGRKQP"
     gene            complement(14279..14512)
                     /locus_tag="ACFM4Z_000626"
     CDS             complement(14279..14512)
                     /locus_tag="ACFM4Z_000626"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020702.1"
                     /translation="MRRRRRSSKTQLPDGTQKLPSGGHPDGKAGIGADAVRDMCHAEL
                     SAWFEDILSSLGIKTPKEEGVIVSKRLRKPEGK"
     gene            complement(14487..14852)
                     /locus_tag="ACFM4Z_000627"
     CDS             complement(14487..14852)
                     /locus_tag="ACFM4Z_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020703.1"
                     /translation="MITVKLTHGLTYNGKVVSELRLKPLTVGGELAAFALIDDLPELP
                     ENATKAELLQRDVLETLTYWSQQIEAQGIPSDILTAQWLMENLSTEDYHTVMAAQEDL
                     RLKPSAATASPDAPSAAEQ"
     gene            complement(14856..15233)
                     /locus_tag="ACFM4Z_000628"
     CDS             complement(14856..15233)
                     /locus_tag="ACFM4Z_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFC6020704.1"
                     /translation="MSDATYADAVIMEMNGRDIEIVSIKPQTTTGRKPVKTMNRNGRV
                     NGYCDGVTEHKLSVTAAIPIDGTEIDWDNITKAKITIYPINDEDRRTSYLDCFTVDTG
                     EQYEVDNEARIDIEMIALHKIKE"
     gene            complement(15298..16707)
                     /locus_tag="ACFM4Z_000629"
     CDS             complement(15298..16707)
                     /locus_tag="ACFM4Z_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFC6020705.1"
                     /translation="MPHIDFDTIPGSIRVPGQYIEFNTRNAVQGLPQNPQKVLMVAPM
                     LTAGIQPALEPVQLFSDAEAADLFGQGSLAHLMVRQAFANNPYLDLTVIGIADHSAGV
                     QATATVTLSGTATAPGVVEITIGGKQVSTAVNTGETAATVADRLKTAITAADVTVTAS
                     GSGAAVTLTAKHKGEIGNESGLTVSTGNTGLTYQANAFTGGAKNADIATALSKVAGKH
                     YHIICSPFSDDANAKALSNHITNVSNAIEQRGCIGVLGMSAALSTATTATGEINDGRM
                     TCAWYKGAVEPNGIIAAGYAAVLAFEEDPAKPLNTLEIKGLAVTPDAQWPLFAECNNA
                     LYNGLTPLTVVNNRVQIMRAVSTYTKSANNTDDPALLDITTIRTLDYVRRSVKERIAL
                     RFPRDKLSDRLLPKVKSEILDVLIKLDQAEIIENAEANKGKLVVARAQNDPNRVNAII
                     PADVVNGLHVFAGRIDLIL"
     gene            complement(16710..16907)
                     /locus_tag="ACFM4Z_000630"
     CDS             complement(16710..16907)
                     /locus_tag="ACFM4Z_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFC6020706.1"
                     /translation="MSKIKVTAADGLRVPTEHNPHEYIGQEPVEVDGNSLYYRRMIDD
                     GDLVVVEDAAPNTKTRNTKGE"
     gene            complement(16904..17554)
                     /locus_tag="ACFM4Z_000631"
     CDS             complement(16904..17554)
                     /locus_tag="ACFM4Z_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp37"
                     /protein_id="HFC6020707.1"
                     /translation="MITDIEQAITDRLKRGLGRMVRTVKSYNGEADDLAGQIHTLPAV
                     WVTYGGSKVEPASTGGVCGRYQDTAEFVVMVAARNLRNEQAQRQGGIDSREIGSNDLI
                     RAVRRLLDGQRLGFADSRGLVPKAVRAIANHVLVQNAAVSIYAVEYAIRFNTCGLEND
                     RYPERTDNPDDPNHIFTKYQGTLSEPWPDFEGLDGKIYDPQSADEIPVNLTLKDKQ"
     gene            complement(17544..17966)
                     /locus_tag="ACFM4Z_000632"
     CDS             complement(17544..17966)
                     /locus_tag="ACFM4Z_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp36 family protein"
                     /protein_id="HFC6020708.1"
                     /translation="MAYATVEDMVARFGELEVLQLTDRNNEGLIDREVAQTALVDATA
                     EIDAYLGRFRRPFEDLPPILVRLCCDIARYRLTAAQGVLITDEIRNRYKIDVLDLLRA
                     MAKGEVQLGVDDSGEEVAAGEDGIVFVNGKNKVFGRDH"
     gene            complement(17966..>18374)
                     /locus_tag="ACFM4Z_000633"
     CDS             complement(17966..>18374)
                     /locus_tag="ACFM4Z_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020709.1"
                     /translation="GVIMAKTNNKPETAETAAPSFEDIKAELDAVQAELAAARNDVEM
                     LTTALEKAEDDKKALSAELAELKVQHTQRAADALADSRDVMLVSTGADGKEFWRGGLL
                     FDGGWREVKRAEVGEAVWKAICAEPMLQRKAVE"
BASE COUNT         3618 a         5258 c         4974 g         4524 t
ORIGIN      
        1 gcaacgctgt actggtttaa atttaatcca ctataaatta acacgcaatg taccctgacc
       61 ggcaaaactt ccccaaactt catcaaaaaa tcaactgtac ccgtcttttt cagacggcat
      121 tgataccgta agaaatttcc catgcagaaa tggcaaaccg agctttattc cacaccgctt
      181 tggctttgtc tgacaccgaa aagcgcggtc aaagtcttgc tgctgattac ggcgatgatt
      241 acgctggtgt taaccgaaat gcggctgaat gtgttgagta cctttatgtc caaagggctt
      301 tacgactcga tgcaggattt gaaatgcttc ggcattttgg atgtttgctg caatgaacgc
      361 gggcgtggtg ctgatacggg cgtttaacaa cgtcgtcaac gacttcctcg atcaaggctt
      421 ggcgattaaa tggtcggagc ggctcaacga agtgctgaca gcacgctggc ttgccgacaa
      481 aaactactac cgcctgcaaa tgcgccgcca cgcgccggac aacatcaacc agcgtatcca
      541 acaggacgcg caggaattta tcgcttcgac catcgaattt gtgcgcggca tggtcaattc
      601 ggtcgttact tctctggaat ttgccgttgt tttgcggggc ttggctggca tcctgaccgt
      661 gttcggcttc gacattccgc acggcatcgt ttggtttgtc tatatatttg tgattttggc
      721 gacctttatc gccatgtgga taggcaaccc tttgattcgt tacaattatg aaaacgaaaa
      781 actcaacggc gactaccgtt attccctcat ccgcgtgcgc gaccacgccg aaagcgtggc
      841 gttttacagc ggcgaaaaac acgaacacga ccagctttct gaccgtttta aagccattat
      901 ccgcaaccgc tggcgtattg cgcgacaaag cgtctgcctg agcggcttta atgatatgtt
      961 caccaacggc atcaagctct tccccattat tttgcaagcg ccgcgcctgt ttgccggaca
     1021 aatcaaaatc ggcgacatcc agcagaccgt ccaagccttc gcgcgactgc aaaacgcgct
     1081 ttctttcttc cggatgttct acaacaaatt caccgcctac cgcgcccgat tggagcgtct
     1141 gtacggcttt ttgctgagta ccgaagaaca acacagcgcg cggcaacccg agattagcga
     1201 agtttcagac ggcattgcac tggaatgtaa ggcggcggca gcttttgcac gaattggcgg
     1261 cagcttttgc accgccgaag cgtaggaaat tgtattagct tttgcacaaa tttatccccg
     1321 tttaatggtc tgttaaacga ggattatctt atgcctttaa aagtagtacg ctacttgcgg
     1381 tcagaactta taaatcaggt ggtcgtcgta gcttgaattg ctgttgatgt gctgctcaca
     1441 gtcaaggttt ggctgcctgc aaaacgctcc catccggtca ggcgcagttc tgccacgagg
     1501 tcaagataag caggcagttc gctgcgggtg ccggagaaga agacaaatgg cggccgcacc
     1561 attgccatca ggcgcagaaa ctctaccatg ccgaagtagt tcatcgcgtc cccttttccc
     1621 cggcggcgcg gctttggttg aaccgcgcgt cgtgctgcct gcctttcagt tcgctgatat
     1681 acgcctgccg gcagtggtgg cggtcgaaaa acacgccgtt gatcagggta tataaaatct
     1741 tccagcgggt tttgggcttt tgggcgagcc gcgcgcggcg ataggtgcgg cttgagaggg
     1801 tttcgtccgc cgcgccgccg gttagggcgt tgaacagttg gtcgatggct accaacaggt
     1861 ggtaaacgta ttttttcagc cgatgtttag cgtccattct tcgatttcct tttccagcgc
     1921 gtccaatccg ggcgcggttt cgatggtgtt caatttgtct tcgagctgct gacgctttcc
     1981 gataatcgcg ccggcggcaa cagccaggcg ggcggatttt tcgataactt tttcaatcaa
     2041 aacgtccaat tccacgcccc ttgcggcggc gatttgcgcc agcatcgggg tcggggcgtt
     2101 gttgtccgcc tgccgcgcga gggcttcttt ttcctgcctg taaaagctgt cgatttccac
     2161 ttggggatag cccgccaaga ggctgttttt gagttcgtcc gccttttccg cgaggcggaa
     2221 tgccaaggcg gttttttgtt tggcgaaacg ggcggcggcg gcggctttgc tgattttcca
     2281 ttttttgccg tcccattcgt ggtaatcgga cgggcgcggc ggggttaaaa cggggcggcc
     2341 gtcggaatct gcggcaatct gcccgccctg cgcctgtcct gccaaaaggg cggcgtattc
     2401 ttcggcgcgg acggcaaccg cgccttcggg gatgccgccc aatgtgtcgt cgtaaaagcc
     2461 gtttttgaaa tagatggtca ttttggggtt tccttttttg tatttacatt ttggtttttt
     2521 gccgcttata atgtaacgac aaaatacgct gcaagctgca atgaagattg aatttgactc
     2581 cgaaaaaaat caacgcaata tcgaagagcg gaatttgccc tttgaatcag tcgggcaaat
     2641 acgctggaca acggccgtta ttgtgcccga tgtccgcttt gattaccccg aaccgcgtta
     2701 tgttgccgct gcttatctag gcgatacgca acgcctgcac attgtctgtt ttacgccgat
     2761 aaaagacggc atccgtgtca tcagttttcg caaagcgaat aaaagagagg ttaaaaaata
     2821 tgcaacgtca atccttaaca aacgctgacg gcgaagtgcg ggaacttgcc gccgaagatt
     2881 ttgcgcttgc ccgccccatt gccgaagccc tgcccgaaga cttggcacag gtgctttttt
     2941 cccatcaaaa acaattggaa gaaaagggcg taatgcaaaa acgaaacaca ggcaaaaccc
     3001 cgaaacaact cgttaccata cgcctgtccg ccgatgtggt cgaaaaattc cgcgccggcg
     3061 gcaagggctg gcagacgcgc atcaacgagg ttttgcgcca atatatcgcc caattgaaat
     3121 aacgggggcg gccgctgctt atcgggcgcg cccgactatc ctttcccgcg cggtttcggc
     3181 cgtgcggttt tttgttgccg gtgccgtccg aagggggcag cggctttttg cgcggtcatt
     3241 cccgcctttc gttattcccg ccactttccg tcattcccgc gaaggcggga atcccgaaac
     3301 ccctgccgcc gtcattcccg cgcaggcggg aatccagtcc gttcggtttc agccgtttcc
     3361 gatagattcc tgccgcgttt ggggtctgga ttcccgcctg cgcgggaatg acggtggcgg
     3421 ggggtcggtt ttttccgata gattcctgcc gcgtttgggg tctggattcc cgcctgcgcg
     3481 ggaatgacgg cggcggggat ttctgttttt tccgatagat tcctgttgcg tcggggtctg
     3541 gattcccgct ttcgcgggaa tgacggcggc gggggtttct gttttttccg atagattcct
     3601 gccgcgtttg gggtctagat tcccgctttc gcgggaatga cggtggcggg ggtttcggtt
     3661 tttccgatag attcctgttg cgtcgggggg tctggattcc cgctttcgcg ggaatgacga
     3721 aagcggcggg aatgacgaag agtggcagga ttggcggggc ataggtttcg ggggattggc
     3781 ggcgcggccg gtttcggtcg gattgcttgg atcggcgggc tgaagcccat ctctacaact
     3841 cgtctctaca acccatctct acaacccatc tctacagcca tctctacagc catctctaca
     3901 gccatctcta cagccatctc tacagcccat ctctacagcc catctctaca gcccatctct
     3961 acagcccatc tctacagccc atctctacag cccatctcta caacccatct ctacagccca
     4021 tctctacagc catctctaca gccatctcta caacccatct ctacaacccg tctctacagc
     4081 catctctaca gcccgtctct acagccatct ctacagcccg ctagtcttgc agtgcctcga
     4141 gcaagccgtc tcggcggcgg cggtttcggg gtccggttga tgttcttcca ctctctcttt
     4201 gcggttcttc gttggtttca ggtatttcgg ggcctatcgg ggagctggcg gcgtttcccg
     4261 aggcggcttt gccgatggcc atccagtcgc aactgccttc gtaccagttg gtgataatgg
     4321 cggcggcgtg gttttttgcg cggataaaca gccagttgtg ttgccttcga tcgccggtaa
     4381 cgcgttccga atggcgttcg gatacgaagc atttgacgtt gccgtcggca aaggcgacgg
     4441 ggaagacgac ttcgttttcg atggggccgc cgcttcgtgt gaacctgtag ctgccggtct
     4501 gtatcattgt gccgtcgggg aatctgacaa tatccacgcc gccgatggtt tggcgtgtga
     4561 attgcgcggc aacggcggtg ctgatttttt cgtccagccc ttggatttgg gcggcggtgt
     4621 gggtgtggtc tcggtcggct ttgtcttgca aaccttgcgc cagcgtgccc gcgtccagcg
     4681 cgcctgcgtt gatggtttcg ccgtgggact ttatccaaaa cacggcttcg cccaaggtgt
     4741 cggcggcttt gatgcacagt tttaaaacca gggctttggg gcggttttcg tcgccgcctg
     4801 tcgccatttt gctgtccgag cgcggggtta aaaagccgtt gtcgtttaaa ttttcgtccg
     4861 tccaagtcga tacgagcgcg cttctctgcc tttcgttgcg gtcttcgtaa ccgagggctg
     4921 ccgcgtctgt gtggtttgtc cagtgtgaaa atactttgtg gacgtgccgt ttgatttcgt
     4981 cttcctgctt cgttccgact gccaagctgt tgcccgcgtt gcggataaag cggtcttccg
     5041 cctgcgggac gttttggatg ctgccgtatt ttccggtcag cagacggtac agctcgggat
     5101 aagcggtttc ggttacgcgc gtgcggatgt cgtcaaacgc cagccagccg gtcgggattt
     5161 ggtcggacgg aaaccacgcg gtgatgccga tgtcggtacg gcttaaatcg ggcaggcggt
     5221 tgctgttgcc caaggcgcgg taaaggtcgg ggaaggtgtt ttgtgcaaag gtcgtgccgt
     5281 tggctttgag atagcctgcg gggttttgta cggctttggg aaaggatacg atcgcgccga
     5341 cggggatgcc gtccgaattg agttttttcc atgcggacca gttgtcgttt gcgctgctcg
     5401 tttggtggcg ttcgtacacg tcggacgtgt aggcgggata acccaactgc ctgcaccagc
     5461 cgtgtttcgt gccggcgatg acttggatat ggcaggcggt gttttcaacg ggcaggttgg
     5521 agctgccgac cgccgtcggc agggaataga tgccgtctgt tttgagggtg ttcaaatcgc
     5581 cccggaatgt ttcgactttg aaattgccga tgccgtatcc ggacagtgtg ttcggtttgc
     5641 cttggatgtc ttcggcaaaa cgcggtttgt tggctttgtg gaagatgtcg tggttgttgt
     5701 aggaaatgcg gttgtttgcg ccgtgcaggc ggagggtgtc gtcgccgaag acgatgtggg
     5761 cgttgtcggc atccgcgccg atgtaggctt tgtggccgcc gttccagttc gtctggttgg
     5821 cggataaatg gatgccgctt tggaattggg tttgggcggt aaaggttttt tggccgttga
     5881 cggtttggtc gccggtgata actacagcca gcctatttag tgctgccagt aattgattag
     5941 gctgcgactt atccggctgg ataccggccg cagtcagcac ggccagcagc tcggattgca
     6001 cttggttgag ccaccacgcg ggcaggatgg tgcccaattc ggacacgccg tcgccgtcgt
     6061 ggaaggtctt atcgggggtt tcgatggggt gcataaatcg tcctttagcg gtaggtaaat
     6121 cggatagcgg tgtgggcggg cttgaggcgg ttgaacaggc tctcgatcac ggcatcgctg
     6181 taatcggtca gcctgtcgcc cgccgccgag ataccggcgc ggaatcgggt aatgcggttg
     6241 ttgccgccgc gcacgttaac gtgccacacc cacatgattt cctgcggcgc aagacggtcg
     6301 ccggcgcggt ttacaccggc gcggaacggc tgcggttcgt cgatttggat ttgatagccc
     6361 gccgcctcgg ccaaacgcac aaaataagga atactcaagc cgcctgtttc gtttagcttg
     6421 gccatgacgg ccaacacacg gtgctggcgg tttttgcccg taccgtccaa acctaatacg
     6481 cgctcccaat cggccagcat ttggccggcg ctgcgcgggt cgacggcatc ggcaacgctt
     6541 tgagccgatt ccgccaccgc atccagcgct gcgccgtcta tttctgcctg cgcccgcaca
     6601 cgcggggcat tgcgggcata cgacacgggg gcaacaggcc ccgcaagatg tcttgatagc
     6661 tcatgacggc atctccgtta cattaatcgc gcccgcttta aaccactcga tgcggttaac
     6721 cgtatcggcg gcacggttgg ccgtcggcgc agtcagacgg cggtcgatca caccatccac
     6781 attgctgata gcagcctcga tttgcgacac agtcaggccg tcgccgggaa tcagggtgtc
     6841 gaaatattct tttagggccg tccgtatgcg gcgcttggcc tcgtccaagt cgataccgtc
     6901 gagcttgact tgcacggtaa caggcaccgc cgttacggtt ggcttgagta ccagcgcatt
     6961 ttttgccgtt accgggcgca tctcgtcgat ataagcctgt acgcggcgca cagtttcttc
     7021 cgacgacaca ccgtcggcgg aggtaatggc aatatccacc gtacccaagc cgcggcgcag
     7081 cggataaaca tatgcgctgg ttacgccgtc aacactcaac gcccagtttt tatagtcgta
     7141 acggttgccg cctgcgggcg gtcggcggat gatttccaac agacgcgcca gcagtgaggc
     7201 atcgctttct cggtcggtgc cgccttgtac ggtaaggcgg cattcggtgg ccacaccggc
     7261 gggggcggcc atcagttgcg cctcgccgtc gccacattgg cggccgcgcc cggctcgtcg
     7321 gcgattgccg gtatttccgc cgtgccgccg ctgccgataa cggcgcgggc ggtagtgcgg
     7381 taaaaacgct ggccgatacg cacttgcagg tcgtctgaaa gcatggattg cgcaataccg
     7441 cttacggtca gcgtgccgct ggccgtggta ggattgcggc ggctcaagcc gcgcatggag
     7501 gcatgccgct ccaaatactc gcggtcggcg gtatcaggga aaatctgccg cacaatccag
     7561 ctttgatgcg catattgccc ttcggcgcag ctggccaaac ggctggcgtg cacataatgg
     7621 tcgctgtcgg ggctgatatc ggcatccggc cacaggcttt tggtatcgcg caggatacgc
     7681 tcgcggattt gctcaaatgt cggcgtttca aacacgttta aaacctcttt aaatcacggg
     7741 cacttcgtgg cggtaatcaa acccgcccgc cgccgtatcc acccggatat gcaggatcag
     7801 gcggccgtcg tgcggctgct ctgcgcgcac ggtaatcttg tcggcacgcc cgctcttaac
     7861 aatcggttgc agtgcctcat cggcatattg ctcggccaac aggctgaccc gttgcaggtc
     7921 tttttcgcgc tgcaacaaat gcagcagcga gccgagcgtt ttatccgccc accagctgcc
     7981 caacggtgtc atcaaacgga tatacacggc attttgcagc gtatcgacgg tgcggccggt
     8041 atagtcgccg gtgccggggt ttagctcttt gtccatgttg gcattttgcc gttttcagac
     8101 ggcctcacgg agtacacggg cttcaatccc gcacgcacaa aaaaagcagc ccgaaggctg
     8161 ccaaaggtct gcttgtctat gccggttccg ctggtaaggt tttgccgccg atgctgtcgg
     8221 tatgcgggtg ctggcgcaac gatatattgc cggccaccac gtcgccgtcg gtgttaaagc
     8281 tgccgcccgt ttggttaaca tcgccgctaa aggtggctcc gtcgccgccc tcgacggcca
     8341 tgccgccgtt gccgttgatt tggccttgcg ccgtcaacac tgcactggtc tccaccaacg
     8401 gagcgttaaa tttggcatcc gtggccgcat taacctcgta ttgtttgcag ttaacccggt
     8461 acacgtcgca atcggcctca atgattttgc cttgcttaat cacgattttt gcaccctcat
     8521 gattaaaaat cgccgtctcg ccgggcttaa ggtttttgat gcggtagctg ccgtgctggc
     8581 tgcacacaat cacaccgtgc gaagtattgc cgcccagcgg tatcactacc gcttcgctgc
     8641 cgtcgggcgg atggctggca aagccgtatt cctgcaaatg ttcaaggtct tgcagggttt
     8701 cgtcggccaa gccgctcaac tgcacgcgct gtatcggctc ggacgacacc accagcgtga
     8761 tttttccccg aaaggccgcg cgcagggttt cgccgatatt tttagcagtt tgtgccgttt
     8821 ttttcgccaa tttactcaaa ctcattcaaa caccgccgtt tctgcttgtt ttttgccgcc
     8881 ttttttgccc ttatggctca cgcctttgcg tttgcctttg cgcttgcgcg ccgcctcggc
     8941 ctttttgggg taagcgtcgg gtgtccaaat accgtcctct ttgagccgca gctcggtttg
     9001 cgtgccatcc atgcgggata gcataaaccg ccgccccatc agaaaaaaca ccgcatcgat
     9061 accgtgctcg tcgtcgatca catgcacacg ctggccaggt tgccacaata cgccgtcgcg
     9121 ggttttatgg ccgcccacgg ttatcgtgag tgtcaatccc tccagccgcc agtcggccag
     9181 ctgcttttta gcctgctttt gcaatgcggc caaattgtcg gcatcggaca ccaccaccgt
     9241 tttagggcgg tgcagcgtca tcgtcgggtc tttgtacacc cacttaaatc gtgtttggcg
     9301 ctgtcgccgc tgcggccgtg cgattgcgcc aaaaaagtaa cctcggaaaa gcggttgtcg
     9361 gtatcccact caatgtccat gcgctcgata ttgcggcggc tgtcggtgcg gctccaacac
     9421 aatgtcgcca ccggcgggct gctgtaatcc gcaccgccca ccaccaacgt gccgtccggc
     9481 tccagccacg gatgcagccc gaccgagttg gcaatatggg ttaatgcctg ccatacggtt
     9541 tcgcccggct cgatgtcgat tttgtccaaa gcggggttgt tttcggcctt aagcaccacc
     9601 gctttaatct gcggccacgg cgcggccagc tttttggctg catccaatac cgtcatgccc
     9661 tttacattga gctgcggcgc ggagcaatcc accaaaaaac cggccaaatc acgcccgctc
     9721 aagctcaact cgcggccgcc cttgcttttg ccgtggcgct ggctgccgat gatgcccgtc
     9781 atcacgattt gcccgtctat cactacctcg cagctctctc cgcttaaatc gggtatggcc
     9841 gcctccggcc ccaacctgcc gatgacaaaa tcgaagctgt cggcagggat taaaaagtcg
     9901 ctgtcgatgt cgtagcgctc ccagtggcgg tgctctttac cgcccacgcg caccgacacg
     9961 gcatagccgt atgaattatt ttgcatagct gttgaccaaa gtgccgcgct tgataaacgc
    10021 ggggtggtgg atatggggat tgagccgcac cagctctgct gcgcgggcta tatcgccgta
    10081 aaactcgtgg gcgatttggt gtatcgtacc gtcgattggg gcttggcgca caatcagcgg
    10141 cggcttttgg ttgatgaccg ccgcaaccaa cgcattcaga cggcctgccg ccgcgcgcag
    10201 ggattctgcc gtttggtaag cctcggtata cacggcgttg gccgtcagcc caccagactc
    10261 ggcagcagcc gtctgcaccg cccgtaaggc ggcaatctcg gcctgcatac ggcggcgcat
    10321 ggcgcggtta acctcaatca aatcgggcgc ggtcatctct tcgccatgcg cctcgattaa
    10381 tgccgttgcc accgacaaca gcgatgacgt ggataacagg cgcaccgcct gcgctaccgg
    10441 ctgcacctgt ttggcggtta aacggttcag gcggttttgc aggccgtctg aaaagcggcc
    10501 ggtcagcaaa ttatcaggga tagcggcggc acggtcggca acagccgcag ccccgtcaaa
    10561 ccgctgtcgc ggcgaccaac cggcatggtg catggcatta tcggccaaac ccgcctcacg
    10621 gcgtatgcca gtatctacca tgaccgatat atccgcaaac agcttggccg agccgttttt
    10681 aaacgccgct gcgctgtatc cgccccgatc gggaaaggca attttgtcca aatcaaacaa
    10741 acggcgcacc gcgccaaacg tacccgatgc cgcactccaa atgcccagcg cgctgcccca
    10801 taaagccgat acgcccgcat ccaccgccaa caccgcatca acaaagccga tagccgcctc
    10861 gcggtaggtg tcgatatcag caatcaacgc ctcaagctcg accaaaaagg cgttttcaaa
    10921 aacaaagatt tcctgcgctt cggccgcctc gcggaaagta atatcgatgc ccgcataatc
    10981 cacataatcg gcctcatgtc ggtagctcca tgatgccgca atcatgttgt gcatccgccc
    11041 ccaaacaggg tgcaccagca cgccgccgcc cggctgctcc agcgcatcca gcagcttttt
    11101 cagacggcct gcatagccct tgccccaaaa caccgcatta atctgcacct gccgcccggt
    11161 cgtgcccatg tcttcaaggt cgataccctg cacaaacggc cgcgcatgct cggccaatgc
    11221 cttgccgttg ctctcgtcca ccacctcaat atcaaagccg acgcccttgt aagatgcgtc
    11281 ctgcaataag gtatgccatc cgctcattat tgacccgctc cacggccgaa catggccact
    11341 tgataccgcg atacctcatt cgcgataaca cgtccgtcga tattcaggct cacattggta
    11401 ttgatggtct gacttgccga cgccatcgca ctttgcactt gtgccaaacc tgttgtaact
    11461 gcagccgtat cctgcgccaa tgctgcctga tacgccgccg tctgctgctg aatggctgcc
    11521 tgatacgccg ccgtctgagt tgaaaacaac ggagacaaca tcttatcggt aggctgcgct
    11581 gacggagcag ccaaatactg agggacacca ccccgcgcct gttcccgctt cggcgcaggt
    11641 tcaaatcgga taccgttgtt tttcagacga ttaatcattt caggagttgt cttggtacca
    11701 cgcaaaccct ttggcaatgt gccgtcaccc aaagactcgg aatacagcaa catacctaac
    11761 aaacccgccc gtttaactaa cgctggatta ttcatgagcc cgctaccggc agattttccc
    11821 catgacagca acttaccgcc tgccgctgtg gcacctgctg ccacgccgcc tgcggaagcc
    11881 ttaccccatc ccaacgcttt actacctaca tctttcagaa agcctgcacc tttaccgcca
    11941 ccggcggtta ataacattgc ggcagacgcc gctgttgccg ccgtcgtcaa tgcttgcagg
    12001 gctagagtgg cattagggta ttccgctgcc agctttttaa acgaggtttc catatccacc
    12061 aatgttcccg tcagactttc ccgcaacatt gccagcgatt cctgttgctc ttgttgcgcc
    12121 gctgccgaca acattcgcgc ctcgttgttc ttagcaattt ttccgttatc caacgttacg
    12181 ccagccaacg aagccatata ttcacggatt tgcgtcatat ccgttgcagc cagcaaacct
    12241 tgttttgcct gcaaatcagg cagcagttgc gccaaaagcg cgcccttaag catatttgcc
    12301 tgctccgccg ccgtcttatc gcctgcagcc gcgcgtttct tataatcttg gtattgctta
    12361 tcctttacta gcatggcatc ggcaagacgg gacaacacct gcactgcgtt ttcgccgttt
    12421 tgcttgcctt gcacaaccga gcctatccaa tcgacacctt tcttcgggtc attcggattt
    12481 gccatcttct tcagacgacc tatcgtgtca ggcgacagag ttttactcaa aagattttgc
    12541 acattagtcg ccgcttcggc aggactgccc gatttattcg ccgcagattg taagagtgag
    12601 agcaggtagt ccaaaccgcc gacaccattc atccctgcct gttgcgcggc agggagcagg
    12661 ctcggcagct cccgaaccat atcccgcacc tcgaaagtgc cgtctaaacc cgattgcaag
    12721 acgtgctcaa gcccgagttg caggtcttta ccgctcatgc cgccatcttt cagggtttta
    12781 atcagttttg ccgtatcctc gccactgcct tctgaggcaa gtgcaaaagc atatgccgcc
    12841 tgcgcttcat tcttggtttg ggcaaaattc agactggtgg tcatcatgcc gctgattaaa
    12901 tccaaagcct tatcgtgggt cccgccattt ttctcgacaa gttcaagtgc caaatccttg
    12961 atctgttgcg caccttcagt tgcaatccac gctgccgatt tactgttatc ctcaataaat
    13021 gcctgtctgg acacgcggtt gatgttctca tcaagctgct ttctgttgtc catagcaggc
    13081 ttaagcaccg tatacgctgc cgcgccacca gccaccgcag ctgcaccgaa tcttccgatt
    13141 tttcccatct tgtccgcaaa tcccgtgccc tgtttcagtt ccgcgttcag ctcgcgaatt
    13201 cggttacgcg tagcgaccgc cgcccgtgcc aaatcatttt gtgatgcctt gccgctgcgc
    13261 gccaaacggt taaacgcagc ctgtgtcagc atgatttcgc gttgaatctg tttttctgag
    13321 cggatgccgg tcaacgcata agtatggctt tgccgttcaa gttgcgcccg cgatttcgcg
    13381 gtacgatcta attgtcgttg agtatcagcc agtagccgtc tcgctccgtc atctcgggcg
    13441 gttaacacca acgacagttt catgttcccg tttgccataa aaagcagcct ttaaacgtat
    13501 taactcaagg tcaattatcg tttaaaggct gtttaaggga ggcggtatgg gcttcaaccc
    13561 cgttttcgtg ctttatttgt ttgttgcgtt ataatactag aatgataacc attttattca
    13621 aagggatatt ttatgaatat gaaaaaattg atttccgcaa tttgtgtttc aattgtttta
    13681 tcagcctgca accaacaatc aaaaacggca caagccgaag aacctgtcca aagtatccag
    13741 gctgctgatt gtaccgcccc aatggacatc acagttgaac aatatctcat caatttggag
    13801 caagcattta aaactcagaa cgtctcaaca aaaatccata ataaaaatat tgtcaagacc
    13861 gattgtggtt atgaccttac tttggtaatg gattttgggg cgattgcgct caaactggac
    13921 gagcagcaaa aaattagagc tatctcagta ggctacattt taaaaaccga cggagagaaa
    13981 ggacaaaatc tagtcaataa tgccataaat ggattacaca gtattcaggc agttctgtct
    14041 ttaactacca cagacaaatt gggcgaatcg gaagcaggaa aacaactttt tacagcttta
    14101 accgaagtcg tcaaagaatc caatcagaca ggagcaacag cgcaaaaaga cgttccggca
    14161 gatggtattt tatatagcgt tgtttttgaa aaagaaacaa acaccattgc aataatcggc
    14221 agaaaacaac cctaaccgtc aaataaaaaa aaggtcgtct gaaactcaga cgaccttttt
    14281 attttccctc cggctttctc agccgtttcg acacaatcac tccctcttcc ttcggcgttt
    14341 ttatgcccag gctcgacagg atgtcttcaa accatgccga cagctcggcg tggcacatat
    14401 cgcgcactgc gtccgcccct atccccgcct tgccatcagg atgaccgcct gacggtagct
    14461 tttgtgtgcc gtcaggtagc tgcgttttac tgctccgccg ccgacggcgc atcggggctc
    14521 gccgtagcgg cggacggttt taggcgcaaa tcctcctgag ccgccatcac ggtatggtag
    14581 tcttcggtag agaggttttc catcagccac tgcgccgtca ggatgtcgga tgggatacct
    14641 tgggcttcaa tctgctgcga ccagtaggtc agcgtctcta ggacgtcgcg ttgcagcagt
    14701 tcggcttttg tggcgttttc gggcagctcg ggcaagtcat caatcagggc gaacgccgcc
    14761 agttcgccgc cgacggtcag tggcttgagg cgtaattcag atacgacttt gccattgtag
    14821 gtcagcccgt gggtcagttt gacggtaatc acgcattact ccttgatttt gtgcaaagca
    14881 atcatctcaa tgtcgatgcg tgcctcgtta tcgacttcat attgctcgcc ggtatcgacg
    14941 gtaaagcagt cgaggtagga agtgcggcga tcttcgtcgt tgatggggta aatcgtgatt
    15001 ttcgccttgg tgatgttgtc ccagtcgatt tccgtaccgt cgatcggaat ggcggcggta
    15061 acgcttaatt tgtgttcggt tacgccgtca caataaccgt tgactcggcc gttgcggttc
    15121 atcgttttga ccggcttgcg gcctgtagtg gtttgcggct tgatgctcac aatctcgata
    15181 tcgcggccgt tcatctccat aatcaccgcg tcggcatagg tagcgtcgct catgctttat
    15241 ccttttttaa ggtttcagac ggcctttaaa cctgttttaa acgccgtctg aaaagggtta
    15301 caaaatcaaa tcaatgcgcc cggcaaagac gtgcaggccg ttgaccacat cggcggggat
    15361 aatggcatta acacggttgg ggtcgttttg cgcacgcgcc accaccagct tgcctttgtt
    15421 ggcctcggcg ttttcgatga tttcggcttg gtcgagctta atcagcacgt ccaaaatctc
    15481 gctcttaacc ttgggcagca ggcggtcgct caatttgtcg cgcggaaaac gcagggcaat
    15541 gcgctcttta acgctgcggc gcacataatc cagcgtgcgg atggtggtaa tgtcgagtag
    15601 tgccgggtcg tcggtgttgt tggccgactt ggtataggtg gatacggcac gcataatctg
    15661 cacgcggttg ttgaccactg tgagcggggt caagccgttg tacagcgcat tgttgcattc
    15721 tgcaaacagc ggccattgcg catcaggtgt aacggccagc cctttgattt ccagcgtgtt
    15781 cagcggcttg gcagggtctt cttcaaaggc caacaccgcc gcataacctg cggcgatgat
    15841 gccgtttggc tctaccgcac ctttgtacca agcacaggtc atgcggccgt cgttgatttc
    15901 gccggtagcg gtggtggccg tgctcaaggc cgcactcata cccaatacgc cgatacagcc
    15961 gcgctgctcg atggcgttgg atacgttggt aatatggttg ctcaaggctt tggcgttggc
    16021 gtcatcgcta aacgggctgc aaataatgtg ataatgcttg cccgccactt tggacaaggc
    16081 cgtggcaatg tccgcatttt tggcaccgcc ggtaaaggca ttggcttgat aagttaggcc
    16141 ggtattgccg gtgctcacgg ttaagccgct ctcgttgccg atctcgcctt tgtgtttggc
    16201 cgtcagcgta acggctgcgc cgctgccgga tgcggtaacg gttacatcgg cggcagtgat
    16261 ggcggttttc agacggtctg ccactgtggc ggcggtctcg ccggtgttaa cggccgtgct
    16321 tacctgcttg ccgccaatcg tgatttccac cacgcccggc gcggtggccg tgccggaaag
    16381 ggtaacggtt gcggttgcct gcacgcctgc gctgtggtcg gcaataccga taacggtcaa
    16441 atccaaataa gggttgttgg caaatgcttg gcgcaccatc aaatgcgcca gcgagccttg
    16501 tccgaacaaa tcggccgcct cggcatcgct aaatagttgc accggctcta aggcgggctg
    16561 tatgcccgcg gtcagcatgg gtgcaaccat caataccttt tgcggatttt gcggcaaacc
    16621 ttgtacggca ttgcgggtgt taaattcaat atactgcccg ggcacgcgga tgctgcccgg
    16681 aatcgtgtca aaatcaatat ggggcatcat tactctccct tagtattgcg ggttttggta
    16741 tttggggcgg catcctcaac caccaccaaa tcgccgtcat caatcatgcg gcggtaatac
    16801 aggctgttgc cgtccacctc caccggctct tggccgatat attcgtgcgg gttgtgttcg
    16861 gtcggcacac gcaggccatc tgccgccgtt actttgattt tgctcattgc ttatccttaa
    16921 gggttaggtt tacaggtatt tcatcggcgg attgcgggtc gtaaattttg ccgtccaacc
    16981 cctcgaaatc aggccacggc tcgctcaatg taccctgata cttggtaaag atatggttgg
    17041 ggtcgtcggg attgtcggtg cgttcggggt agcggtcatt ttccaacccg caggtgttaa
    17101 agcggatggc atactcaacc gcatatatgc ttactgcggc gttttgcacc agcacatgat
    17161 tggcaatcgc gcgcaccgct ttgggcacca agccgcggct atcggcaaaa ccgagccgct
    17221 ggccgtcaag caggcggcga acagcgcgga ttaaatcgtt gctgccgatt tcgcggctgt
    17281 cgatgccgcc ttgccgctgc gcctgctcgt tgcgcagatt gcgggccgcc accatcacca
    17341 caaattcggc ggtatcctga taacgtccgc atacgccgcc ggtgctggca ggctcaactt
    17401 tgctgccgcc atacgttacc caaaccgcag gcagcgtatg gatttgcccc gccaaatcgt
    17461 cggcctcgcc gttgtagctt ttaaccgtgc gcaccatgcg acccaagccc cgtttcagac
    17521 ggtctgttat cgcttgctca atatcagtga tcacgcccga acaccttatt tttaccgttt
    17581 acaaacacaa taccgtcttc gcccgcggcc acttcttcgc cgctatcatc cacgcccagc
    17641 tgcacttcgc ctttggccat agcacgcagc aggtcgagca cgtcgatttt gtagcggttg
    17701 cggatttcgt cggtaatcaa cacgccctga gccgccgtca gacggtagcg ggcaatgtcg
    17761 cagcaaaggc gcaccaagat gggcggcaga tcctcaaaag gtcgtctgaa ccgccccaga
    17821 tacgcgtcga tttcggcagt ggcgtccacc agcgcggttt gtgcgacctc gcggtcaatc
    17881 agcccctcgt tgttgcggtc ggtgagctgc aagacttcca gctcaccgaa acgcgcaacc
    17941 atatcctcaa ccgtcgcgta tgccattact cgaccgcctt gcgttgcagc ataggctcgg
    18001 cgcagattgc cttccacacc gcttcgccga cttcggcgcg cttcacttcg cgccagccgc
    18061 cgtcaaacag caggccgccg cgccaaaatt ctttgccgtc tgcgccggta ctgacgagca
    18121 tcacatcgcg gctgtccgcc aaagcgtcgg cggcacgttg cgtatgctgc actttgagtt
    18181 cggcaagttc ggcggacagt gcctttttgt cgtcttcggc tttttccaag gctgtggtca
    18241 gcatttcgac atcgtttcgg gcggcggcaa gctctgcctg cacggcgtcc aattcggctt
    18301 tgatgtcttc aaacgacggg gcggcggttt cggcggtttc tggtttgttg ttggtttttg
    18361 ccatgatgac tcct
//
LOCUS       DAXHNE010000023        17979 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.023, whole genome shotgun sequence.
ACCESSION   DAXHNE010000023 DAXHNE010000000
VERSION     DAXHNE010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17979
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.023"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            135..971
                     /gene="cysT"
                     /locus_tag="ACFM4Z_000634"
     CDS             135..971
                     /gene="cysT"
                     /locus_tag="ACFM4Z_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HFC6020710.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            1226..2086
                     /gene="cysW"
                     /locus_tag="ACFM4Z_000635"
     CDS             1226..2086
                     /gene="cysW"
                     /locus_tag="ACFM4Z_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HFC6020711.1"
                     /translation="MKPYSANPNLTEPRWLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAV"
     gene            2083..3156
                     /locus_tag="ACFM4Z_000636"
     CDS             2083..3156
                     /locus_tag="ACFM4Z_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFC6020712.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGKILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(3213..4739)
                     /gene="ilvA"
                     /locus_tag="ACFM4Z_000637"
     CDS             complement(3213..4739)
                     /gene="ilvA"
                     /locus_tag="ACFM4Z_000637"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFC6020713.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEQEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            4887..6056
                     /locus_tag="ACFM4Z_000638"
     CDS             4887..6056
                     /locus_tag="ACFM4Z_000638"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFC6020714.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNKPAVHAAPTFQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTIAEKEIVALENVEKRSRWQRLWACLTGQ"
     gene            complement(6181..6753)
                     /locus_tag="ACFM4Z_000639"
     CDS             complement(6181..6753)
                     /locus_tag="ACFM4Z_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFC6020715.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQPVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(6820..7803)
                     /locus_tag="ACFM4Z_000640"
     CDS             complement(6820..7803)
                     /locus_tag="ACFM4Z_000640"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HFC6020716.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            complement(7970..8045)
                     /locus_tag="ACFM4Z_000641"
     tRNA            complement(7970..8045)
                     /locus_tag="ACFM4Z_000641"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8010..8012),aa:Gln,seq:ttg)
     gene            complement(8054..8129)
                     /locus_tag="ACFM4Z_000642"
     tRNA            complement(8054..8129)
                     /locus_tag="ACFM4Z_000642"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8094..8096),aa:Gln,seq:ttg)
     gene            complement(8142..8217)
                     /locus_tag="ACFM4Z_000643"
     tRNA            complement(8142..8217)
                     /locus_tag="ACFM4Z_000643"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8182..8184),aa:Gln,seq:ttg)
     gene            complement(8225..9070)
                     /gene="ispE"
                     /locus_tag="ACFM4Z_000644"
     CDS             complement(8225..9070)
                     /gene="ispE"
                     /locus_tag="ACFM4Z_000644"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFC6020717.1"
                     /translation="MNIADGRQAFSAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVDGMPQEVDLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFARGIGDRLD
                     EMDIPKQWYVIVKPPVHVSTAKIFTHESLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSDLYEAYLAEGLSKH
                     PLLSV"
     gene            complement(9080..9661)
                     /locus_tag="ACFM4Z_000645"
     CDS             complement(9080..9661)
                     /locus_tag="ACFM4Z_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HFC6020718.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            complement(9801..11645)
                     /locus_tag="ACFM4Z_000646"
     CDS             complement(9801..11645)
                     /locus_tag="ACFM4Z_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6020719.1"
                     /translation="MLPNRFKMLTVLTATLIAGQVSAAGGGAGDMKQPKEVGKVFRKQ
                     QRYSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPE
                     VAERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLE
                     EVLAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSV
                     QGREKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQ
                     EMEIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAY
                     GRGTEEQRSRAALTAAMMYADRRDYAKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGG
                     RAALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALRGLDKIIEKPPAGS
                     NTELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLTDSKRLD
                     EGFALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGE
                     VLWALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            complement(11810..12646)
                     /gene="panC"
                     /locus_tag="ACFM4Z_000647"
     CDS             complement(11810..12646)
                     /gene="panC"
                     /locus_tag="ACFM4Z_000647"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFC6020720.1"
                     /translation="MQIIHTIRELRAWRKNAGKVAFVPTMGNLHEGHLALVREAKKRA
                     DSVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLAAEGIAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFHIVSPDIACFGKKDYQQLAVIKGFVED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLVQGSLDYA
                     GLEKRAVQSLTEYGWVVDYVEIRRADTLEVARAGDKKLVVLAAACLGTTRLIDNLEIK
                     LP"
     gene            complement(12769..13560)
                     /gene="panB"
                     /locus_tag="ACFM4Z_000648"
     CDS             complement(12769..13560)
                     /gene="panB"
                     /locus_tag="ACFM4Z_000648"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFC6020721.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVEMLLVGDS
                     LGMAVQGRKSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVLAELAKKVTETVSCPTIGIGAGADCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMQGHDSVQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            complement(13702..13777)
                     /locus_tag="ACFM4Z_000649"
     tRNA            complement(13702..13777)
                     /locus_tag="ACFM4Z_000649"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13742..13744),aa:Thr,seq:tgt)
     gene            complement(13812..13887)
                     /locus_tag="ACFM4Z_000650"
     tRNA            complement(13812..13887)
                     /locus_tag="ACFM4Z_000650"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13852..13854),aa:Thr,seq:tgt)
     gene            14053..14844
                     /locus_tag="ACFM4Z_000651"
     CDS             14053..14844
                     /locus_tag="ACFM4Z_000651"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFC6020722.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDIILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            14899..15540
                     /locus_tag="ACFM4Z_000652"
     CDS             14899..15540
                     /locus_tag="ACFM4Z_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC6020723.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(15618..16541)
                     /locus_tag="ACFM4Z_000653"
     CDS             complement(15618..16541)
                     /locus_tag="ACFM4Z_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC6020724.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHSLFEPGKVMFYYRETSRESEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            16705..17574
                     /locus_tag="ACFM4Z_000654"
     CDS             16705..17574
                     /locus_tag="ACFM4Z_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFC6020725.1"
                     /translation="MKRIFLPALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGKQPDRIDGKKESARNTSTYAHFGAGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            17617..>17979
                     /locus_tag="ACFM4Z_000655"
     CDS             17617..>17979
                     /locus_tag="ACFM4Z_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020726.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQAFEKYVFHTQADAPMQLA
                     ELSQKEMKETEGEWIPNAVGGIMGGVGGHFSYMASAIASGSYNREAHWTAIGGGALIG
                     AMNPINGGRALINGMRGVA"
BASE COUNT         4378 a         5157 c         4280 g         4164 t
ORIGIN      
        1 acatcgcgcc cgcttgagaa actgccaacc gctttataac aaattcgtct ttgcaccaaa
       61 ctttccattc tttccgtttt tcggacggca tcgttaaagt agtccttcct tttccttatt
      121 ttcagcattg ttttatgtta ccgctcaaaa cgcccggcgt actgcccggc ttcaaactca
      181 gccttggtct gaccgtattg tgcctgtcgc tgcttgtggt cttgccgttt gcgatgatgg
      241 cggcgaaggc ggcggaaatc ggctggggcg gcttttggaa cacgattgcc gagccgaacg
      301 tgttggcggc ggtgtggctg agtttgcgga tgtcgtttta tgcgatgctg accaatgtcg
      361 tgttcggcac gctggtggcg tgggtattgg tgcgctatga atttccgggc aagggcttgg
      421 cgaacgcgct ggttgatttg ccgtttgcgc tgccgacggc ggttacgggt atcgcgttgg
      481 caaccctgta tgcgcctaac ggttggatag gccgtttttt cgagcctttg ggcatcaaaa
      541 tcgcgtttac gcccgtcggc atttggattg cgctggtcgt cgtcagcctg ccctttatcg
      601 tccgcgccgt gcagccggtg ttggaggaat tatcgggcga atatgaggaa gcggcggcga
      661 ctttgggcgc aaaccgcttt acgacgttcc gccgcgtgct gctgccggaa atcacgccgg
      721 cactcttgac cggcgcggga atgatgttcg cgcgggcaac gggggaatac ggctcggtga
      781 tttttatcgc gggcaatatt ccgatggttt ctgaaatcct gccgctgatt attacgggca
      841 agctggaaca gttcgacgta cagggcgcgt cggcggtggc gttgtttatg ctgctggttt
      901 cgtttgtgat tctgtttgcg ctgaacgtga tgcagtgggc gttgggcagg cgttcgggcg
      961 cgaagggttg aggtcgtctg aaatacctgt taccgtcatt cccgcgcagg cgggaatcca
     1021 ttggtgaatt tcagctgcct tatttatttt ctgttttcct gttgccctgc ggtggattcc
     1081 cgcctgcgcg ggaatgacgg tagctagacg tttttattcc cttaatcaat aaaaggttgt
     1141 ctgaaaacga atccgcccca caaaaaacgg tttttcagac ggcatccgaa caccttaaaa
     1201 caaccaaccg gggaacacca ccgccatgaa accctattcc gccaatccca acctgaccga
     1261 accgcgctgg ctgcgcgtgt tgctgattgc cgccgcgctg ggctttctgc tgctgatgct
     1321 ggtcgtgccg ctcgtcgccg tgttttacga agccttaaaa ggcggttggg atttgtacct
     1381 gaaatcctta aacgatcccg aagcgtggtc tgccatcaaa ttgacgctga ttaccgcgct
     1441 gattgtcgtc cccgtcaatg ccgtcttggg cgtggcgatg gcgtggctgc tgacccgttt
     1501 tgattttcgc ggcaagcagt tgctgactac cctgctcgat ttgccgtttt ccgtatcgcc
     1561 cgtggtggcc ggtttaatgt tcgtcttatt gttcggcgcg cacacggcat tgggtggctg
     1621 gctcgaagcg caaggcatac agattatctt cgccatcccc ggcattattc tggcgacgct
     1681 gttcgttact ttcccctttg tcgcacgcga aatcatcccg ctgatgcagg ctcagggcga
     1741 cagcgaagaa caggcggcat tgatactcgg cgcaagcggc tggcagatgt tttggcgcgt
     1801 taccctgccc aacatcaaat gggcgttact ctacggcatc atcctcacca acgcccgcgc
     1861 gatgggcgag ttcggcgcgg tcagcgtggt atcgggacac atacgcggcg aaaccaacac
     1921 cgtcccgctt ttggtcgaaa tcttctacaa cgaatacaac ttcaccggcg cattcgccct
     1981 ctccggcgta ttggcacttt tggcactggc gacgctggcg gtgcagaaca tcattaccaa
     2041 attacaagac aaaaaactcg ccgccgccga aaggaacgca gtatgagtat caccatccaa
     2101 aacttaaaca aacacttcgg caattttcac gcgctgaaaa acatcaacct caacgtcccc
     2161 accggcaaac tcgtttccct gctcggcccg tccggctgcg gcaaaaccac gcttttacgc
     2221 attatcgccg gactggaaaa cgccgacggc ggcaagattc tgtttgatgg gcaagacgta
     2281 accgccaagc acgtgcgcga gcgcaaagtc ggcttcgtgt tccaacacta cgcccttttc
     2341 cgccatatga acgtgtttga caacgttgcc ttcggtttga ccgtattgcc caagtccgaa
     2401 cgcccgtcca aaggacaaat ccgcgccaaa gtcgaagaat tgctgaaact cgtacaactc
     2461 tcgcatttgg caaaatccta tccgcaccaa ctctccggcg ggcaacgcca gcgcatcgcc
     2521 ctcgcccgtg cgcttgcggt cgaaccgaaa ctcctgcttt tggacgaacc cttcggcgcg
     2581 ttggatgcca aagtacgcaa agaattacgc acctggctgc gcgacatcca tcacaacctg
     2641 ggtgtaacca gcattctggt tacgcacgac caagaagaag ccctcgaagt ttccgacgaa
     2701 atcgtcgtga tgaaccacgg caaaatcgaa caaaccggca gcgccgaagc tatttaccgc
     2761 aaacccgaaa atgccttcgt taccgagttc ctcggcgaaa ccgacgcttt tgaaggacgc
     2821 atcgaaaaag gcttctggca ttataacggc ttcgcgtgga aattggacgc acaatacaaa
     2881 tggcaggaac aaaccgccac cggctatatc cgcccgcacg aatggcagat cgccgccgaa
     2941 cacgaaacac cgatgatttg tgccgaaatc gaaaaagtcc acgccgtcgg ggcattgacg
     3001 catattttgg tgaaacacgg caaacaagac gtgcatatca cgctggctgg cagcgatgcc
     3061 gcgcgttacc caatcgccga aggcaaagaa ttgaagctga ttccgaaaca ggtttatgtc
     3121 ttctctcaaa acgaactgat tgaatattcg atttaaccat gaaagcgcaa tgccgtctga
     3181 aaggctttca gacggcattg tgctttcaag cgtcaggcaa gaaacagctt gtacgcggca
     3241 ttttgcgttt cctcgtgata gctgtatccc agactttcca agaaaccgtc aaatgcggcg
     3301 gcatcgtgcg gcggcacatc gataccgacc aaaatccgcc cgtaatccgc accgtggttg
     3361 cggtaatgga agagcgtaat gttccaaccg ccctgcatat ggttcaaaaa acgcgccaac
     3421 gcccccggac gctccggaaa ctcaaaactg accagacgct cgttttctac tttgtccgtc
     3481 cgccctccga ccatatagcg gatatggatt ttggcaatct catcatcggt caggtcgaca
     3541 ttgggcaatc ccgcctcatc caaccggctg ccgataaccg ccaaatcctg cgggcctgcc
     3601 gcttgaagtc cgacaaagat atgcgctttt tcatcgtccc cgtagcggta gttgaactcg
     3661 gtaatattcc tatttcccaa tatattgaca aacttaagga agctgccgcg ttcttcaggg
     3721 atggtaacgg caaaaatacc ttcgttgccc tcgcccaatt cgctccgttc cgaaacgtgg
     3781 cgcaaacggt gaaaattcat attcgcaccg ctggtaacgg caatcagggt ttggttttcc
     3841 gcgccttctc gggcgatata ggctttcaga cccgccaacg ccaacgcgcc cgccggctcg
     3901 gtaatgctgc gcgtgtcatc gaaaatatcc ttgaccgcgc cgcaaaccgc atcggtatcg
     3961 actgtaatga tttcatccaa aagttctttg cagagacgga aggtttcgtt tccgacgact
     4021 ttgaccgccg tgccgtctga aaacagcccg acatctttca aatggacgat ttcacccgct
     4081 tcgaccgact gcttcataca gcaggaatcg ttggtctgaa cgccgataac tttgatttcg
     4141 ggacggacct gcttgataaa tgccgccacg cccgccgcca aaccgccacc gcctatcggt
     4201 acgaatacgg cgcggattgg atcgggatgc tggctgacaa tttccatccc taccgtccct
     4261 tgtcctgcaa tcacatcagg atcgtcaaac ggtgcgatat aggtcagccc ttcctgttcc
     4321 gccagctcca tcgcataatc gtaagcatcg ttgtatgaaa caccgcgcaa aaccacctcg
     4381 ccgccacggc tcttaaccgc atccactttg attttcggcg tagtctccgg cataacgata
     4441 acggcacggc agcccaaacg ctgtgcggac aatgccacgc cttgagcatg attgcccgcg
     4501 cttgccgcaa tcacgccgca agcgagcgca tctttcggca acttggacat tttgttgtac
     4561 gcgccgcgta ttttgaacga aaaaaccggc tgcaaatctt cgcgtttcaa aaggatgttg
     4621 tttttcaaac gtacagaaag gctgcgtgcc ggttccaaag gcgtttcgac cgcgacatca
     4681 tagacagatg ccgtcaggat gcggatgagg taattggaat aaggaagggg cgtgttcata
     4741 attcaatatg ggataatcgg tttattaaaa tcgcaaaacc caaaaccata cgcccaagac
     4801 ggcgcgaaag caagaaaaat ccgcccgatc agacacccta agcgtataat cggcagactg
     4861 aaacacgcac acaattagaa tatttcatga cagcacataa aatcctgccc gtcctgcttt
     4921 ccatcatctt aggcgtttct cacgcaacgg ccgcatcgcc cgcgcccaac aaaccggcgg
     4981 tacacgccgc ccccacgttc caaacacccg aaaccctcac agcggcacac atcgttatcg
     5041 accttcaaag caaacagatt ttatccgcca aaaacatcaa tacccctgtc gaaccggcgg
     5101 cactaaccca actgatgacc gcatatctgg ttttcaaaaa catgaaatcg ggaaatatcc
     5161 gatctgaaga aaacttaaaa atacccgaat ccgcatgggc ttcagaagga agcagaatgt
     5221 ttgtacgtcc cggcgatacg gtcagcaccg acaaactctt aaaaggcatg attgcactat
     5281 ccgcaaacga tgccgcccta acccttgccg gccggctggg caacggctcg attgaaaatt
     5341 ttgtgcaaca aatgaacaaa gaagcccgac gcttgggcat gaagaacact gtattcaaaa
     5401 atccgacagg cttgagtaga gaaggacagg tttccaccgc caaagacctc gcccagctgt
     5461 ctgaagcatt gatgcgcgac tttccggaat attacccgct gttttccatc aaatctttca
     5521 aattcaaaaa tatagagcaa aacaaccgca atatcctttt atatagggac aacaatgtaa
     5581 acggtctgaa agccggacac acagaaagcg gcggctacaa ccttgccgtg tcatactccg
     5641 gcaacggcag gcacatcctt gtcatcacat taggttcgga atcggcggaa acacgcgcct
     5701 cagacaacag caagctgctg aactgggcat tgcaagcctt cgatacgccc aaaatatatc
     5761 cgaaaggcaa aaccgttgcc caaatccaaa tttccggagg cagcaaaaaa accgtccgcg
     5821 caggcttcct caaagaagcc tacatcactc tgccacataa ggaagcgaaa atggcagaac
     5881 agattctaga aaccatacag ccgattcccg ccccggtaaa aaaagggcag attttaggaa
     5941 aaatcaaaat caggcaaaac ggatacacca ttgccgaaaa agaaatcgtc gcactggaaa
     6001 atgtagaaaa aagaagccgg tggcaaaggc tttgggcgtg tctgacaggg cagtaatctg
     6061 ccgtttcaaa tatcccgttt ttccaacaaa taaagaaatg ccgtctgaaa cacggttcag
     6121 acggcataaa acaacagggc ggtacgtatt gcatacgcac cgccctgctg ctgaaatcaa
     6181 ttagcgtttc ttaccggtaa cggtagcaac agccagattt tcgttacgtt tcagggaaac
     6241 gctttctaca ccttcaggca gtttgatgtc tgacaagtgc agaatgtcgc cggcaaccac
     6301 ttcggcacaa tccaaatcca agaaagcagg gatgttggca ggcaaagcaa ctacttcaac
     6361 agaagtgttt aacagagata cgcggccgcc ttgcagtttg accgcttggg aattttcagc
     6421 gttaacgatg tgcaggggaa cgcggatgcg tacgggttgg tctgctttca cagcttggaa
     6481 gtcgatgtgt tgaacttcgc ggcggaacgg gtgcatttgg aaatcgcgga cgataacgtc
     6541 tttggtttca ccgttcagag acaacttaat caacgcagta tggaaagatt ctttttccaa
     6601 tgcgtagaat acggttttgt gatccacagc gattgcaaca ggctcttgac cttcaccgta
     6661 cagaatgccg gggatttggc cttcgcgacg caggcggcgg ctcgcaccag tgccttgtgc
     6721 ttcacgaaca gaggcttgaa tttcataagt catgttaaat actccaagtt aggtaaaatc
     6781 gccgtcatcg gccgcgacca gcttaagacg gcttcgggct tatggcagca acatgctgcc
     6841 tgtcatcact tcttcattga aaagatatga gacggattct tcattgctaa tgcggcggac
     6901 ggtttcggcc aacagaccgg caatcgttac ctgacggata cggtcgcagt ttttagccgc
     6961 ttcagacaaa ggaatggtat cggttacgac cacctggtcg atttcggatg aggcgatacg
     7021 gctgaccgcc tctccggaga atacggcgtg gctggcatag gctagaacac gttcagcccc
     7081 ccgctctttc agggcgacgg cggctttgca cagcgtattt gcagtgtcaa tcatatcgtc
     7141 cacaatcaga caggttctac cttgaatatc gccgatgatg ttcatgactt ccgccacatt
     7201 ggctttcggg cggcgtttgt cgatgattgc caagtcggca ttcagggatt ttgccacggc
     7261 gcgggcgcgg acgacaccgc cgatgtccgg gctgacgacg gtcagatttt caatccgctg
     7321 ttgtttgatg tcgttcaaca gaatcggggt ggcataaata ttgtccaccg gaatatcgaa
     7381 gaaaccttga atctggtcgg catgcaaatc gacagtcaaa acacggtcga tccctgccga
     7441 atacagcata tttgccacca gtttggcaga aatcggaacg cggacggaac gcggacggcg
     7501 gtcttggcgc gcatagccga aatacggaat ggctgtggta atacgacctg ccgaagcacg
     7561 cttcagtgca tccgccatcg tcaggatttc catcaggttg tcattggtcg gcgcacaggt
     7621 cggctgaagg atgaaaacat cgcgcccgcg tacgttttcc aacagttcga cggcaacttc
     7681 gccgtctgaa aacttggata cggaagcatt gcccaaagaa atgtccaaat gcctgacaac
     7741 acgttgtgcc aattcgggat tggcattgcc tgtaaatacc atcaaactgt cgtacgcagc
     7801 catattctca cctgatttta tgtttaactt ccgctcagaa aacacaatgc ttccctctgc
     7861 ttgtaccaag cccgacatta tacctgcaac atccgacaca aacaaaggca tttcaatatt
     7921 tttatttcta tgaaataaaa gcgtgtaagc aggcttacac gcttttattt ggctggggag
     7981 gaaggattcg aaccttcgca tgctggaatc aaaatccagt gtcttaaccg cttgacgact
     8041 ccccaaaaag ggctggctgg ggaggaagga ttcgaacctt cgcatgctgg aatcaaaatc
     8101 cagtgtctta accgcttgac gactccccaa ctcgtttgac ttggctgggg aggaaggatt
     8161 cgaaccttcg catgctggaa tcaaaatcca gtgtcttaac cgcttgacga ctccccaaca
     8221 atgtttatac ggacaacaaa ggatgttttg aaagaccctc tgccaaatat gcctcgtaca
     8281 aatctgaaac ttgtcggtat atattgtatg cgctattcct atcttgacac gccgtgaata
     8341 cacacgcacc ggaacctgtc attaaggcaa atccatatcg ggacaactcg gaataggctt
     8401 tccaaacttc agggtattct ttaaatacaa ctgcctgcat atcatttcta aacggttgca
     8461 gattttggaa agtcggcatt atgcttgagg cggaatttcg tgtcaagctt tcgtgtgtga
     8521 aaatttttgc agtggaaacg tggacgggcg gtttgacgat gacataccac tgtttcggaa
     8581 tatccatttc gtccagcctg tcgcctatac cccgcgcaaa cgcatttttg ccgaaaataa
     8641 aaaacggtac gtccgccccc agagccgcgc ccgaatcaat gagctgccgc tgcgtcagac
     8701 cgcactgcca ccaacggttc aacaccagca aaacggttgc cgcatccgag cttccgccgc
     8761 ccaaacccgc ccctgtcggg atttttttgt ccagccatat ttccacgccg gcggggttgc
     8821 gcgcatattt ttgcagcaac gatgcggcac ggtagctcaa atctacttcc tgcggcattc
     8881 catcaaccgg attgtgcagg atgattttgc cgtcgtccct cggtttcaaa tatacggtat
     8941 cctgcaaatc tatcaggcag aatatgcttt cgatattgtg ataaccgtct tcccgcctgc
     9001 cggtaatcct caaatcgaga ttcagttttg caggtgcgga aaacgcctgc cgtccgtccg
     9061 caatattcat ctgtccgcct tatctcgtgc gcgccgcaca gcgttccggg gtttcggttt
     9121 cagacggcat accgatttcg gtgaaaacca gcctgatgtt caaatttccg ttattcagtt
     9181 gcaacgttcg gacttgcccc ccactgtcgg cggttctgcc gacagtccaa ccgtattgtt
     9241 ccaatatgcc gtccggcagg atgcggtaag gcgcgcccgc cacacgcctg ccatctgccc
     9301 agatatgcag atattggatt ggcagtttga aaccgaccag ctgcctgctc aattcttccg
     9361 cactttccgc ctgatagaca tttcctttgc cgtccactgc caatgcgccg tccctgtctt
     9421 gacacaactg cccgagcgta ctgcccaaag gggtattgat attgatggtt tccacgggcg
     9481 gttggtatgt ccaatcgaaa tttgcatacg aacctttccc ttccgctttc actgccaacc
     9541 gcccttctgc tgcaaaactg ctgatgtgtt cggacggctg ccacaggttt tcgttatttt
     9601 gaggtaattg cgcgcaagcg gtcaaaagca ggatgaccga tgcggatacg gtgtgtttca
     9661 tcagaatttc cttaacggat gccatcctgc cggccgatgg gttcggcgtg ccgtgcaaaa
     9721 cgcgtgcgaa ctgcgtgccg tttgcccgaa tgccccggct tcccggcaaa ccttatgccg
     9781 tctgaaagga tggacctgca ttatttccga ggttttcggg aaggttgggg caatgcgatg
     9841 ccgtgacgtt tgagcgtttc ccgccatatt ttcttgtctc ccgtaaggtg tgccgcctgc
     9901 gtccatacgt caaccgcctg atcgcgttcg cccaatgccc acaacacttc gcccaaatgg
     9961 gcggcaactt cgggctcggg gtcgttttca aacgaatacc gcagatacgg cagcgcgctt
    10021 tccgcgtcgc ctttcaggta atacgcccag cctatgctgt cgttgacagc ggtatcgtcc
    10081 gggttgattt ggtatgccgt ctgaagcagg gcgaaacctt cgtccaaacg tttggaatcg
    10141 gtcagcaggc tgtagcccag attattcata atctgagcgt tatcgggtgc aagcctgaac
    10201 gccctttcaa gatctgaaat catttttttc cgcttgccaa gccgatcgta aacaactgac
    10261 cgctgtacca atgcctctgc ctgtaactct gtattactgc cggcaggcgg tttttcgata
    10321 atcttgtcca accccctcaa agcctcccgt ttatcgggca gcttcgacag ggcgagcatc
    10381 tgtattttgg acaaattgtc tgccgtaaaa taccgcccct gctgttcggg aagtttccgc
    10441 accctgccga tctgccgcaa agccgccctg ccgccgtcca actcgacagc cgccgcagcc
    10501 gccagcacac ctttgtcgaa caggtattcc ggcgcggata cttttttcag ccactgcctg
    10561 actttggcgt aatccctgcg gtcggcatac atcatcgccg ccgttagcgc cgccctgctc
    10621 cgctgttcct ccgtccccct gccgtatgcc ttttcggcgt agccgtcgat aacggaagca
    10681 ccttcttttc ggtttgccgc caatatcgct gcctgaatat acaggtctgc attcggattg
    10741 cgttccaaca gcacgttcaa acgcgcatag gcatcatcca gcctgtgcag ggaaaccaga
    10801 ttcataattt ccatttcctg ccagacggcc gaaaggtttt gggtgtctgt ctgctcgaaa
    10861 aagccgtcga gtatttcggg atatttgcgt gcagtcagac gcaacgtcat taaagtgggg
    10921 ggcaatattt ccgtatcgag cttcgccaaa cgctgcaaag ctccgattgc cttttccttt
    10981 tcccgtccct gtacgctgaa caccacatcg gcaaccgccg cttcgggcag atgttcatat
    11041 ttcaacgccg cgcggcgaac cgctttcgat gctttttgcg ccaacccgtc ctgttgcacg
    11101 gcggcttgtg ccaacaataa aaacaccctg cggttctgtc cttcgtccgc ctgagccagc
    11161 acttcttcca gtccgtccag atgctgattt cctctttccc tcagcacgtt ccgcagccac
    11221 cccgcccgtt tttgcgcctt acccggtata ggctcaatct gccgccattt ctgataaatc
    11281 atttccgcct gttcaaacgc gttcagcgac acggccattt ccaaggcgcg ttcggcgact
    11341 tcgggggatt ttgtgcgttc caacatcagc atataggttg ccagagccgt tcccgcctgc
    11401 cccttttgca aggcggtttc ccctcccagc aacgtaaata tctgattaac ccgctcgccc
    11461 actgccgcaa gccgtgcgcg ttcgtttttg atttcttcct cgctgtaacg ctgctgcttt
    11521 ctgaaaacct ttccgacttc cttcggctgt ttcatatccc ccgcaccgcc tccggcggca
    11581 gatacctgtc cggcaatcaa ggttgccgtc aacacagtta acattttgaa acggttaggt
    11641 aacataatca tcctttatcc gacagccgtc cgaaccttca gacggcatat aatcaggcgt
    11701 aactttatca caagcagaat cgtttgtaga tttgcgaaac cccgttcaaa aaaataaatg
    11761 ccgtctgaaa cgcaaatccg cttcagacgg cattcccgac gcttgcggtt taagggagtt
    11821 ttatttccaa attgtcaatc aggcgcgtcg tccccagaca ggcggcggcc aagaccacca
    11881 gtttcttatc tcccgcccgc gccacttcga gcgtatcggc gcggcggatt tcgacataat
    11941 cgaccaccca gccgtattct gtcagggatt ggacggcacg tttttccaaa cctgcataat
    12001 ccaaactgcc ctgcaccaag gattcggcaa cagcctttaa ttcgcggtac aggcgcggtg
    12061 cttcgtcgcg ttccgccgca ctcaaatact ggttgcggct cgacagtgcc aacccgtctt
    12121 ccgcgcgccc tgtatcaaca ggcactattt caacatcaaa attcaaatct tcgacaaaac
    12181 ctttaatcac ggcaagctgc tggtaatcct tcttaccaaa acaggcaatg tccggggaaa
    12241 cgatgtggaa caatttagaa acaaccgttg ccacaccgcg aaaatgcccc gggcggaatt
    12301 tgccgcacaa ctcattttgc agattgggcg gttcgacgtt gtaacgctgt tccacgttcg
    12361 gatagagttc tttctcatcg ggcgcgaaaa caacggcaat gccttcggcg gcaagtttgt
    12421 ccgcatcctg ttgcaaagtg cgcggatatt tgtcgaaatc ctcgccctga ccgaattgca
    12481 ggcgattgac gaaaatgctg accacgacac tgtccgcgcg ttttttcgcc tcacgcacaa
    12541 gcgcaagatg tccttcatgc agattgccca tggtcggcac aaatgccacc tttcccgcat
    12601 ttttacgcca cgcgcgcagt tctcgaatgg tatgtatgat ttgcataacg gtaatcctta
    12661 tccttcgggc ttcctgcccg acaaaacgcg ccgattatac gcgccgcgca cggcaaaaca
    12721 aaatgccgtc tgaaacggct ttcagacggc atgctgcatg taaaccgctc aatctgcaaa
    12781 aatatgttcc gcagcaggga aggttttggc tttgacttcg gcaacatacg cccgaaccgc
    12841 cgcttgaaca ctatcatgcc cctgcataaa gtttttgacg aatttcgccg tcttacccgg
    12901 gaaaatgccg agcatatcgt gcatcaccaa aacctgcccg tcgcaatccg cacccgcgcc
    12961 gatgccgatg gtcggacagg aaacagtttc agttaccttt tttgccagtt ccgccagtac
    13021 gcactccatc agcacgaccg ccgcccccgc atcgtcatgc gccttggcat cgttaagcaa
    13081 cgcctgcgcc ttgccgccgc gcccctgaac tttatatccg ccgaaggcaa acacggattg
    13141 cggggtcaga ccgatgtgcg cacaaaccgg aataccgcgc atttgcaaaa attcagtcgt
    13201 ttccgccatc cacacgccgc cttcgagttt aaccatatgc gcgccggcag ccatcagttc
    13261 ggcggcggcg gcaaacgcct gctccttact ctgctgatat gcaccaaacg gcaaatcgct
    13321 gacaatcatc gcattttttg caccgcgtgc tacacattcg gtgtgataac acatatcgcg
    13381 caggctgacg ggcagcgtcg atttccgccc ctgaaccgcc atccccaaag aatcgccgac
    13441 cagcagcatt tccacgccgg catcgtccat cagcgcggca aaactggatt cgtaagcggt
    13501 cagcatagcg attttttcgc ccgccgcctt cattttttgc agtgtgttca cagtaatcat
    13561 cagatattgt taccccgccc ttttcagacg gccttttcag tttgaaacgg gctattatag
    13621 tgaaatgccc gcagccctgc caccgaccgt gccgcaaatg caaaacagcc cgactgtttt
    13681 acacaatcgg actgtcaaat ctggtgccgg caccaagagt cgaactcggg accccctgat
    13741 tacaagtcag gtgctctacc aactgagcta taccggctta ctgaaatgtt ttgcaacacc
    13801 atcagcagaa atggtgccgg caccaagagt cgaactcggg accccctgat tacaagtcag
    13861 gtgctctacc aactgagcta taccggcaaa gaaagcgaat tatgcaggca atgccgcacc
    13921 ttggcaagga atttatccga aagccccggc aaatccgaat aagtaattta tttgaaaaga
    13981 aattaatttg aaacagaaca agcatcggca ttctgataaa atatccgcct ttcaaaccga
    14041 ccgttcacac cgatggcaag acatccctac cgccgcctgc gtcctgcaaa atccggcttt
    14101 cccgaagtcg gcatttccga agaaggcaat atccgttcgc ttcacttggg cagcgacacc
    14161 gttcaaagct cgatgaacct cgaccacccg tccgagctgg tgctctctta cagccgcgcg
    14221 atgatgggct ggctgctgtt taccgacgct cttccgcaac atatcaccca aatcggcttg
    14281 ggcggcggct cgtttgcacg ctggatagat acctacctgc ccgacacgcg ccaaaccgcc
    14341 gtggacatca atcctcaggt catcgccatt gcccgcaacc tgtttgagtt gcctttcgag
    14401 ggcgagaaat ttgaaattat tgaagcagac ggtgcggaat atatcaaagt cttccgccac
    14461 aacaccgata tcatcttggt ggacgggttc gacggcgaac aaatcatcga tgcgctggtt
    14521 gaagaaccgt tcttccgaga ctgccgcaac gcactctctt cagacggcat attcgttacc
    14581 aactggtgga gcggcgacaa acgctatcaa cgcttcatcg aacggttgtt gagcgttttt
    14641 gaagggcgcg tcttggaact tcctgccgaa agccacggca atgtcgcggt aatggccttc
    14701 caaagcagcc ccaaagagca aaacatagac aaactcaaaa aacgtgccga caaactgagc
    14761 aacgcatacg gattggactt ccaccgtatg cttgccggcc tgaaagcgtc caaccccaac
    14821 aacggcaagc atttccacct ttaagcaact atcttcacgg ctgcatcgga acatgcggaa
    14881 aatcaagagg aaatcattat gctgaaaccc gaaaccatac acatatccgg tcctgccggc
    14941 attttagaaa ccatccatat cccgtccgaa caagtaccgg cacgcggtgt tgccgtcatc
    15001 aatcatccca accccctcca gggcggaaca aacaccaaca aagttatcca aactgccgcc
    15061 aaagccttaa gcaaactcgg cttccactgc tacctgccca accttcgcgg cgtaggcggc
    15121 agcgaaggca cacacgatta cggacgcggc gaaacgcaag actgcctcgc cgtcatcgac
    15181 tatgcccgtg cccaacatcc cgaagccccc gaatttgcct tatccggctt ctccttcggc
    15241 ggttatgtcg ccacatttgc cgcacaagcg cgcatacccg atttattgct gctcatcggc
    15301 gcggcagtct gccactatac cggccgcccc gagccgtccg ccgttcccaa tgttgccaaa
    15361 acgctgatga tacacggcgc ggaagacgaa gtcgtcgaaa tcgggaaagc actgaaatgg
    15421 gcggaaccgc aagatttgcc cgtcattacc atcgccggtt ccacgcattt ctttcacggc
    15481 aaactcatcg tcctgcgcga caccatcctc cgcttcgcgc ccgtatgcct gaacggataa
    15541 aacccctttc agacggcatc gataatgtaa aacccgtatc ggcggcatcc gccgatacgg
    15601 gtttgcgttc cctctcatta taggcaaaat ccgctgcaaa acaccctttc ccgacccgac
    15661 aaaggatctg caaacgcaat cttttttgcc aatagcttca aaggtttccg ataatcctcg
    15721 ctgcccgctt cagacggcac gggatagagc gcgtcattca acagcggcat acccaaaccc
    15781 atcatatgca cgcgcaactg gtgtttcttg cccgtatgcg gcgtaaggcg gtaaaggctg
    15841 aattcccccc tgttttcaat cagttcgacc gtcgtatgtg cgtttggttc gccttccgcc
    15901 tcttgcgtcg taaaaaattt ctcacccctc accaaacgcg aaaccacatc gagcggatac
    15961 ggcaaatccg tccttgtcgg cgcaagcgcc tcatacgttt tccataccgt tttgttttga
    16021 aacatcgtct gataggctcc gcgcgtggca ggattatgcg acagcagcat cacgcctgcc
    16081 gtatccttgt ccaagcggtg cagcggcgta atgtcctcaa cattcaaatg ctgcaattca
    16141 ggccgcaaac gcaggcgcgt gagcagggtt tcccgcaaaa acctgccgct ggggatgacg
    16201 ggcagaaaat gcggtttgtc caccacaatc aaatgctcat caatatgcaa aatcttttct
    16261 tcaaacggaa tacgcggctc gctctcacgg ctggtttcac ggtaataaaa catcacctta
    16321 cccggctcga acaaagaatg ttcgtccaac gccgcaccat ccgaaccgac cacaaaaccg
    16381 ctgttcaacc gcctgcgcca atcgtccgcg cccacaaaag gaaagcggat gcacagaaaa
    16441 tgcagcagcg gcagcccgta aaactgcttt tcatgcggca gcaccaaata actgggtttg
    16501 acaccgttca acagcggaag aggattattg cgttttttca tgcggcggat tgtaaccgtt
    16561 tttcacaaac ttttcagacg gcatatcgaa acataattga caaaaacaaa catattgttt
    16621 tttatgattt aaaaatttat ttagacaaga ttatcggata ttcattatta tcagtaaata
    16681 agaattaata tcaataggag aaatatgaag cgcatctttt tgcccgcctt gcccgccatc
    16741 ctgcctttat ccacttatgc cgacctgccc ttgacgattg aagacataat gaccgacaag
    16801 ggaaaatgga aactggaaac ttcccttacc tacctgaaca gcgaaaacaa ccgcgccgaa
    16861 cttgccgcac cggtttacat tcaaaccggc gcaacctcgt ttatccccat tccgaccgaa
    16921 atccaagaaa acggcagcaa taccgatatg ctcgtcggca cgctcggttt gcgctacgga
    16981 ctgaccggga ataccgacat ttacggcagc ggcagctatc tgtggcacga agaacgcaaa
    17041 ctcgacggca acggcaaaac ccgcaacaaa cggatgtccg acgtatccct cggcatcagc
    17101 cacactttcc ttaaagacga caaaaacccc gccctaatca gctttcttga aagcacggtt
    17161 tacgaaaaat cgcgcaacaa agcctcgtcg ggaaaatcgt ggctcatcgg cgccaccacc
    17221 tacaaagcca tagatccgat tgtcctttcc ctcaccgccg cctaccgcat caacggcagc
    17281 aaaacccttt cagacggcat ccgctacaaa tcgggcaact acctgctgct caaccccaac
    17341 atctcatttg ccgccaacga cagaatcagc ctgaccggag gcatccaatg gctgggcaaa
    17401 cagcccgacc gcatagacgg caaaaaagaa tccgcaagaa acacatccac ctacgcccat
    17461 ttcggcgcag gtttcggttt caccaaaacc acggctttaa acgcatccgc acgtttcaac
    17521 gtttcagggc aaagcagttc cgaactgaaa tttggcgtac agcatacatt ttaagcaggt
    17581 tttgattttc caaccttgta atccaaagga gttattatga aaaaacaaat taccgcagcc
    17641 gtaatgatgc tgtctatgat tgcccccgca atggcaaacg gattggacaa tcaggcattt
    17701 gaaaaatacg tattccacac gcaggcagat gcgccgatgc agttggctga gctttctcaa
    17761 aaggagatga aggagactga gggggagtgg attcctaatg ctgttggagg cattatggga
    17821 ggagttggag ggcatttcag ctatatggct agtgctattg ctagtggctc gtataatcga
    17881 gaggcacatt ggacagcaat tggaggtggt gcgttaattg gtgcgatgaa ccctattaac
    17941 ggaggaagag cactgattaa tggtatgcga ggtgtagca
//
LOCUS       DAXHNE010000024        17951 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.024, whole genome shotgun sequence.
ACCESSION   DAXHNE010000024 DAXHNE010000000
VERSION     DAXHNE010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17951
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.024"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(85..1188)
                     /gene="dnaN"
                     /locus_tag="ACFM4Z_000656"
     CDS             complement(85..1188)
                     /gene="dnaN"
                     /locus_tag="ACFM4Z_000656"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFC6020727.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGADDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(1268..2824)
                     /gene="dnaA"
                     /locus_tag="ACFM4Z_000657"
     CDS             complement(1268..2824)
                     /gene="dnaA"
                     /locus_tag="ACFM4Z_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFC6020728.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     VSEGMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPHEPRQAAGSASRPESVA
                     VAKARTDVQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VSASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            3116..3250
                     /gene="rpmH"
                     /locus_tag="ACFM4Z_000658"
     CDS             3116..3250
                     /gene="rpmH"
                     /locus_tag="ACFM4Z_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFC6020729.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            3253..3618
                     /gene="rnpA"
                     /locus_tag="ACFM4Z_000659"
     CDS             3253..3618
                     /gene="rnpA"
                     /locus_tag="ACFM4Z_000659"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFC6020730.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLGH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVHRKFDRATAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            3683..3904
                     /gene="yidD"
                     /locus_tag="ACFM4Z_000660"
     CDS             3683..3904
                     /gene="yidD"
                     /locus_tag="ACFM4Z_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFC6020731.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            4077..5714
                     /gene="yidC"
                     /locus_tag="ACFM4Z_000661"
     CDS             4077..5714
                     /gene="yidC"
                     /locus_tag="ACFM4Z_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFC6020732.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCRIDI
                     KRRNDKLYSTSVSVPLAAIQNGAKAEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIIVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMVAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRTQGEVVS"
     gene            complement(5795..6520)
                     /locus_tag="ACFM4Z_000662"
     CDS             complement(5795..6520)
                     /locus_tag="ACFM4Z_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFC6020733.1"
                     /translation="MSEGMVSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAE
                     KTARAHLKHLGVTMPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVA
                     DPGANLVALAHKHGFEVRPLVGPSSLLLALMASGANGQNFAFKGYLPSEKNERIQSLK
                     ALEQRSRQQNETQLFIETPYRNDVLLADAVENLHPETRLCTATDLTLPTQSVVSKTVA
                     DWRRMKEMPNLKKRPTIFVMYAG"
     gene            complement(6567..7157)
                     /locus_tag="ACFM4Z_000663"
     CDS             complement(6567..7157)
                     /locus_tag="ACFM4Z_000663"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFC6020734.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLAGRFPEALIVGADQVAWCDGRQWGKPMNLANAHKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRKLDELHILRYLEREPDAVYCSCALKSEAL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            7305..7808
                     /locus_tag="ACFM4Z_000664"
     CDS             7305..7808
                     /locus_tag="ACFM4Z_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFC6020735.1"
                     /translation="MSDPNLIDSEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSN"
     gene            7842..8021
                     /gene="rpmF"
                     /locus_tag="ACFM4Z_000665"
     CDS             7842..8021
                     /gene="rpmF"
                     /locus_tag="ACFM4Z_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFC6020736.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            8196..8723
                     /locus_tag="ACFM4Z_000666"
     CDS             8196..8723
                     /locus_tag="ACFM4Z_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HFC6020737.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLKELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            8812..9867
                     /gene="plsX"
                     /locus_tag="ACFM4Z_000667"
     CDS             8812..9867
                     /gene="plsX"
                     /locus_tag="ACFM4Z_000667"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFC6020738.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDEAPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENVGGL"
     gene            10003..10254
                     /locus_tag="ACFM4Z_000668"
     CDS             10003..10254
                     /locus_tag="ACFM4Z_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC6020739.1"
                     /translation="MKTLEKRMKALDKRMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            10281..10489
                     /locus_tag="ACFM4Z_000669"
                     /pseudo
     CDS             10281..10489
                     /locus_tag="ACFM4Z_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218360.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10620..10823
                     /locus_tag="ACFM4Z_000670"
     CDS             10620..10823
                     /locus_tag="ACFM4Z_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020740.1"
                     /translation="MPSEAAWAFRRHFLRLKQHIGNPAPPVHGNLHLKTICMPNHTPI
                     PISVICTLPVLGNHRFFAYNSRI"
     gene            10850..11812
                     /locus_tag="ACFM4Z_000671"
     CDS             10850..11812
                     /locus_tag="ACFM4Z_000671"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFC6020741.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALADAKLNINDIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSAADKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            11859..12230
                     /locus_tag="ACFM4Z_000672"
     CDS             11859..12230
                     /locus_tag="ACFM4Z_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFC6020742.1"
                     /translation="MKRQAFFKLMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWTGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TCILIDYFVPPELFVKLGQHL"
     gene            12256..13182
                     /gene="fabD"
                     /locus_tag="ACFM4Z_000673"
     CDS             12256..13182
                     /gene="fabD"
                     /locus_tag="ACFM4Z_000673"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFC6020743.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAGHAVVKNTFAEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LDFTDAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKQICAESAQDEVVEAVNFN
                     SPGQVVIAGNAAAVERAMLAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDVDKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAVCSALTDAGQVAAFIEAH"
     gene            13320..15185
                     /gene="msbA"
                     /locus_tag="ACFM4Z_000674"
     CDS             13320..15185
                     /gene="msbA"
                     /locus_tag="ACFM4Z_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HFC6020744.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIIAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLFALQTANLQSLIDSSPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            15285..16850
                     /gene="guaA"
                     /locus_tag="ACFM4Z_000675"
     CDS             15285..16850
                     /gene="guaA"
                     /locus_tag="ACFM4Z_000675"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFC6020745.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDGEPNTLDVWMSHGDKVSKLPDGFAVIGNTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNESKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVIALRAVITSDFMTAHWAELPYSLLGKVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            16912..17658
                     /gene="fabG"
                     /locus_tag="ACFM4Z_000676"
     CDS             16912..17658
                     /gene="fabG"
                     /locus_tag="ACFM4Z_000676"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFC6020746.1"
                     /translation="MSTQDLNGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETIESLIADIEKAFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRSGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         4465 a         5477 c         4305 g         3704 t
ORIGIN      
        1 tttccgacag ccgaacaaaa ccccgaatcc gtctgcacgg ttcggggcat atctccaata
       61 cgggcatcgt gctccggaaa accgtcaaat tcgcatcggc atcacgatat atttgaaatt
      121 cggattgttc ggcacggtaa acagcgtcga gcggttggca tcgccgaagg caagctgcat
      181 atcgtcggaa tggatgttgc gcaacacgtc catcagatag ccgatattga aaccgacttc
      241 gagttcgccg ccctgatagg caatctcgat ttcttcgcgc gcttcttcct gctcgttgtt
      301 gctgcacacg acgctcaaca ggccggattg caggaacagg cgcgcgccac ggaatttttc
      361 gttggcaaga atggacacac gttccagcgc gcccaaaagt tcggcacggg acaaaacgaa
      421 aatcttgtcg ttgtccaaag gaatcacgcg gttgaaatcg gggaatttgc cgtcgatgac
      481 cttgctgacg atggtcgtgc cgttgcattg gaaacgcacc tgtttgtcca gcagctcgat
      541 ttgaatcgga tcgtcggggt tgttcaacag tttgaacagt tccagcaccg ttttgcgcgg
      601 caaaatcact tcggcgcgcg gcaaatccgc atcaatcgcg caggctgcat aggcaaggcg
      661 gtgtccgtcg gtcgccacaa ggcgcaactg gctgccctca acctgcatca gcagaccgtt
      721 gagataatag cggatgtcct gcaccgccat gctgtactgc acttgcgaca gcatggtttt
      781 gaaacgctcc tgctccagcg agaaagtcgc gctgatgtcc tcgccgacat tcatcatcgg
      841 aaaatcggcg gcaggcaggg tctgcagggc aaaacgcgat ttgcccgcct tcagcgtcag
      901 acggctgtcg tcccaatcca gcgacaccag cgcaccggca ggcagtgccc gtaaaatgtc
      961 ctgaaatttc ttggcattgg tggtgatgcg gaagtcgtcc gcgccgccct cgggacccgc
     1021 agtgtcgatt tggatttcca aatcggttgc caagagtttg gtctgaccgc cttttccctc
     1081 aatcaggacg ttggacagga tgggcagggt atggcggcgt tcgacgatgc cggtaacggc
     1141 ttgcagcggc ttgagcaggc tgtcgcgttc ggcttgtaaa atcaacattt tcgctcctta
     1201 aatcggttgg attttttacc cttcggacgg catgggcaat atcatgccgt ctgaaaggcg
     1261 cgttcggtca gttttgaatc agaatcagca gtttctcgta atcctgcgcc aactcggggt
     1321 cttcctcgcg cagtttcgcc actgccctga tgccgtgcat aacggtcgta tggtcgcgcc
     1381 caccgaacga atcgccgata gacggcaggc tcaaagtggt cagttctttg gtcaggctca
     1441 tcgccacctg gcgcggacgg gcaatgtttc gtgtccgttt cttaccgagc acatcgctga
     1501 ttttgatgcg gtaatatttc gcaacggcat caataataat atcggcggtg atgactttgt
     1561 gcttctcggc aataatgtcc tgcaaagcgg tacgcgccaa atcgatgtcg atgacgggac
     1621 ggttcataaa gcggctgctc gcgctgacgc ggttgaacgc gccttcaagc tcacgcacgt
     1681 tggaacggat cagattggca atgaacagcg cggcttcgtc ttcaatactg atgcccgccg
     1741 cttccgcctt tttctgcaaa atggcgatac gcatttccaa ttcgggcggc tcgagttcca
     1801 aagtcagtcc ccatgaaaaa cgggatttga ggcggtcgtc cataccttcg attttggcgg
     1861 gtaaaacatc gcaagtgagg atgagctgtt ttttctcgtt atggaaatga ttgtacagat
     1921 agaagaattc ctccatcgta cggtctttgc ctttgatgaa ctggatgtcg tcgataatca
     1981 gcaggtcgta ttgcttgtat tgctgcttga acacgtcgta ggtattgttg cgaaccgcct
     2041 tcataaagct gcggatatag tcgtccgaat gcatatagcg cactttggca tcgggacggt
     2101 ttttcaacag ttcgttgccg accgcctgca caaggtgggt tttgcccaaa cccgtactgc
     2161 catagaggaa gaacgggttg taactctgcc ccgggctttc cgcaatcgcc tgcgccgcag
     2221 ccgccgcaag gcggttgccc ttaccttcta ccaacgtatc aaacgtgtaa tccggagaca
     2281 ggttggtctg ctcgtaacgc gcctcttccg catcgcgctg cacgtccgtc cgtgctttgg
     2341 caactgccac cgattccggc cgggaagcag acccggcagc ctgacgcggc tcgtgcggca
     2401 ggtttttcat acgttccgcc aaaatatccg ccgccgtttt cgacgcagcg ggtttgacag
     2461 gctcttcaga cggcagctcg tccaacagaa cctcctgcac gggcattccc tccgacaccg
     2521 catgcaagga cggctcggca ggttcgacag caccttcaac cgccgccatc tcataacgca
     2581 cgccttctcc cggtttgaat acgaaggcgg aacggccggc agccaactct tccctcaccg
     2641 cttctatttt tccggcaaac tggctcttga gcatattgca ggcaaactgg ttcttgccgt
     2701 acaccaccca tacgccaccc tcctcaccaa cggtaagggg cgcaatccat tgcgcaaact
     2761 gcccttgagg caacatatcg tgaagacggc ggaggcacag cggccaaaac tctgctaatg
     2821 tcatggatag gctcgaatcg gtaaaaatga aatcgaaaac aaagaaaata taatattttc
     2881 aaaaagaaaa caaatctgtt gaacgcacat cggttcaaaa cgcgactgcc cgattatacc
     2941 gactcacgaa tattttatcc acaacccgtg caaaaattta tccacagaaa ggcggcggaa
     3001 atccgcaggc aatcgggcaa tcttcctgca aagtttctat attgattgac aaaagcggca
     3061 aattggagtg taattcacgg tttaattatc tacccattct attttaggaa acatcatgaa
     3121 acgcacttat caaccttccg ttaccaaacg caaacgcacc cacggcttcc tggtgcgctc
     3181 caaaacgcgc ggcggccgcg cagtattggc cgcacgccgt gccaaaggcc gcaaacgcct
     3241 ggcggtataa ttttggacta ccgcttcgga aggcagtacc gcttgttgaa aacggatgat
     3301 ttttcatccg tttttgcatt cagaaaccgc cgcagccgcg acctgctgca agtttcgcgc
     3361 tcaaacggca acgggctggg ccatccccgc atcggtctgg tggtcggcaa aaaaaccgcc
     3421 aaacgcgcca acgaacgaaa ttatatgaag cgcgttatcc gcgactggtt tagattgaac
     3481 aaaaaccggc tgccgccgca ggatttcgtc gtgcgcgtcc accgtaaatt cgacagggct
     3541 accgcaaaac aggcaagggc ggaactggca caactcatgt tcggcaaccc cgcaaccgga
     3601 tgcaggaaac aggcatgatt agaacggtac tctgcaggca aggttcagac ggcaacgggt
     3661 ttcccataca aggaacatcc cgatgaactt tctattgtcc aaactcctgc tgggactgat
     3721 acggttctac caatattgca tcagcccgct gattccgccg cgctgccgtt atacgccgac
     3781 ctgttcgcaa tacgcggtcg aagcggtcaa aaaatacggc gcattcaaag gcggccggct
     3841 cgccatcaag cgcattgcac gctgccaccc tttcggcgga cacggacacg accccgttcc
     3901 ctgacccgac gcaatattca aattgcacgc tttcctttta tttcccatcg gtttctatat
     3961 aatgccgtct gaaacttcgg gctggcggca cgaccgtcgg gtatgaagcc cgcccttatt
     4021 ccccgtctat cggaacacgc aacctgcggc atttccgacc attcaggaaa ctctatatgg
     4081 attttaaaag actcacggcg tttttcgcca tcgcgctggt gattatgatc ggctgggaaa
     4141 agatgttccc cactccgaag cccgtccccg cgccccaaca ggcagcacaa caacaggccg
     4201 taaccgcttc cgccgaagcc gcgctcgcgc ccgcaacgcc gattaccgta acgaccgaca
     4261 cggttcaagc cgtcattgat gaaaaaagcg gcgacctgcg ccggctgacc ctgctcaaat
     4321 acaaagcaac cggcgacgaa aataaaccgt tcatcctgtt tggcgacggc aaagaataca
     4381 cctacgtcgc ccaatccgaa cttttggacg cgcagggcaa caacattcta aaaggcatcg
     4441 gctttagcgc accgaaaaaa cagtacagct tggaaggcga caaagttgaa gtccgcctga
     4501 gcgcgcctga aacacgcggt ctgaaaatcg acaaagttta tactttcacc aaaggcagct
     4561 atctggtcaa cgtccgcttc gacatcgcca acggcagcgg tcaaaccgcc aacctgagcg
     4621 cggactaccg catcgtccgc gaccacagcg aacccgaggg tcaaggctac tttacccact
     4681 cttacgtcgg ccctgttgtt tatacccctg aaggcaactt ccaaaaagtc agcttctccg
     4741 acttggacga cgatgccaaa tccggcaaat ccgaggccga atacatccgc aaaaccccga
     4801 ccggctggct cggcatgatt gaacaccact tcatgtccac ctggatcctc caacccaaag
     4861 gcggacaaag cgtttgcgcc gctggcgact gccgtatcga catcaaacgc cgcaacgaca
     4921 agctgtacag caccagcgtc agcgtgcctt tagccgccat ccaaaacggc gcgaaagccg
     4981 aagcctccat caacctctac gccggcccac agaccacatc cgttatcgca aacatcgccg
     5041 acaacctgca actggccaaa gactacggca aagtacactg gttcgcttcc ccgctcttct
     5101 ggctcctgaa ccaactgcac aacatcatcg gcaactgggg ctgggcgatt atcgttttaa
     5161 ccatcatcgt caaagccgta ctgtatccat tgaccaacgc gtcctaccgt tcgatggcga
     5221 aaatgcgtgc cgccgcgccc aaactgcagg caatcaaaga gaaatacggc gatgaccgca
     5281 tggcgcaaca acaggcgatg atgcagcttt acacagacga gaaaatcaac ccgctgggcg
     5341 gctgcctgcc tatgctgttg caaatccccg tcttcatcgg attgtattgg gcattgttcg
     5401 cctccgtaga attgcgccag gcaccttggc tgggttggat taccgacctc agccgcgccg
     5461 acccctacta catcctgccc atcattatgg cggcaacgat ggtcgcccaa acttatctga
     5521 acccgccgcc gaccgacccg atgcaggcga aaatgatgaa aatcatgccg ttggttttct
     5581 ccgtcatgtt cttcttcttc cctgccggtc tggtattgta ctgggtagtc aacaacctcc
     5641 tgaccatcgc ccagcaatgg cacatcaacc gcagcatcga aaaacaacgc acccaaggcg
     5701 aagtcgtttc ctaaatgccg cagcatgaaa aatgccgtct gaaacctgtt cagacggcat
     5761 ttttattgtt caccccctat cggggcggaa atcttcaacc tgcatacatc acaaaaatcg
     5821 tcgggcgttt tttcagattg ggcatttcct tcatcctgcg ccaatccgca accgttttac
     5881 tgaccaccga ctgagtcggc aacgtcaaat ccgtagccgt gcacaagcgc gtttcaggat
     5941 gcaggttttc caccgcatcc gccaacaaca catcgttgcg atacggcgtt tcgataaaca
     6001 gctgcgtttc attctgctgg cgcgaacgtt gttccaaagc cttcaaactc tgaatccgct
     6061 catttttttc agacggcaaa taacccttaa acgcaaagtt ctgcccattc gcacccgaag
     6121 ccatcagcgc cagcaacaag ctggacggac cgaccaaagg ccgtacttca aaaccgtgtt
     6181 tatgcgccaa tgccaccaaa ttcgcacccg gatcagccac agccgggcaa cccgcctcgc
     6241 tgacaatgcc catactgcgc ccttcttgca aaggtttcag caattccggc aaagtcttca
     6301 aatccgtgtg ttcgttcagc gtttgcaaat tcagttcgcg gataggcatt gtcacgccca
     6361 aatgtttcaa atgcgcgcgc gccgtttttt ccgcttccac gacaaaatcc gtcagcccga
     6421 caatcgcctg ttgttcatgc ggcaacaggc acggcgtgtc aggcgtaccc aaaggcgtag
     6481 gaatcaaata caaaacagga gacaccattc cctcactcat cggttaaaaa tgccgtctga
     6541 acccttcaga cggcataaac gggcagttac agaacctcca cgccctcatt tttcaaaaaa
     6601 tcaaccagac ggaaaaccgg caaaccgatt aaagcattcg gatcggtact ctcaatcctt
     6661 tcgatcaaca atgcacccaa agcctcgctc ttcaacgcac acgagcaata aaccgcatca
     6721 ggctcgcgct ccaaatagcg gaggatgtgt agctcgtcca acttcctcat cacgaccacc
     6781 gtcttatcga tatgccgctg catcctgccc gtaaccgtat ttagcagaac gaccgcgctg
     6841 taaaactcaa tctccctgcc gctcaaatgc atcaacatct tgtgcgcatt agcaaggttc
     6901 atcggcttgc cccactgcct gccgtcgcac cacgccacct ggtccgcacc gacaatcaac
     6961 gcctctggga aacgcccggc caacgaccgc gccttaccct cggcaaggcg caatgccgtc
     7021 tgaggggcgg attcccccaa catcggcgtt tcgtcaaaat cgggggacgc cgcctgaaag
     7081 gcaatgccga gcctttccat ctgttcgcgg cggaaaaccg aactcgtacc caaaatcaaa
     7141 ggcagttcca aacccatccc atcctcctta ccgttgaaaa cacgcccgaa ggggcagtaa
     7201 aatccagcca tgcgccgaaa cacggatacc cgccttcggc gtaccgcaac atttttctta
     7261 aaaatattga cgttagaaca tctaaattat atcatatccc gtttatgtca gaccctaatt
     7321 tgattgactc ggaaattttt gccgccgaag ggcagaacct gcaaggcagt tttctgctgg
     7381 aagaattgga tgaacgcgtc agttcgcacg attatcccgc cgacaggcag accaaaatat
     7441 cgtttacact gaccggcggt cgcgaccggc tgcaacgcct gttcctcgac ctgaacgtca
     7501 aagccgatat gcccctgatt tgccagagat gtatcaaacc catgccgttc atgcttgatg
     7561 aaagcagccg tatcgtcctg ttttccaacg aagagtcctt ggacgaatcc atgcttgccg
     7621 acgaagaact cgaaggcata ctgattgaaa aagaactcga cgtgcgcaca ttggtagaag
     7681 accaaatcct gatgtccctg cccttttcgc cgcgacacga agactgcggc gacaatggga
     7741 cactggaaga agtcaatcgg gacaaaccca acccctttgc tgttttggca ggtttgaaaa
     7801 gcaattgatt aggacacagt ttatttatct aggagcttga aatggccgtt caacaaaaca
     7861 aaaaatcccc ttccaaacgc ggtatgcacc gttcgcacga cgcgctgacc gcgcccgcac
     7921 tgtctgtcga cagcacaacc ggcgaagtac accgcccgca ccacatttcc cccaacggta
     7981 tgtaccgcgg ccgcaaagtg gtcaaagcca aaggcgaata atccctattc gactgactga
     8041 aaaagccaga acattgccat gcaattactg gctttttttg cattggacgc accatccgtc
     8101 caaactttcg ccatacgtca acacacaggg gcaaagcgtt ccgtataata ccccgtgaaa
     8161 atattccaaa agccccaacc accaaggaaa ttccgatgaa acagaaaatc tggtacacct
     8221 acgatgacat ccaccgcgtc atcaaagcat tggcagaaaa aatccggaac gccgacatca
     8281 aatacgatgc catgattgcc atcggcggcg gcggctttat tccggcacgt atgctgcgct
     8341 gttttctgga aattccgatt tatgccgtaa ccaccgccta ttacgacagc gacaacgaag
     8401 gacaggttac cgaagaagtc aaaaaagtcc aatggctcga ccccgttccc gaagccctgc
     8461 ggggcaaaaa cgtactcgtc gtcgatgaag tggacgacag ccgcgtaacc atggagttct
     8521 gcctgaaaga actgctcaag gaagacttcg gtacgatcgg agtcgccgta ctgcacgaaa
     8581 aaatcaaagc caaagcaggc aaaatccccg aaggcattcc ctatttcagc ggcatcaccg
     8641 tagaagactg gtggatcaac tatccgtggg acgcactcga catcgacgaa cacaaccgcc
     8701 ttgccgaggc cggccgaggc tgaccctttc agacggcata ttttccgaac cgatgccgtc
     8761 tgaagcccgc acgacccctg ccgcagaccg aaaacctacc ggagaaaccc tatgattaca
     8821 ttggccgtag atgccatggg cggcgaccaa ggacttgccg ttaccgtacc cggcgcaacc
     8881 gcattcctcc aagcacaccc cgatgtccgc ctgattatga ccggcgacga aacgcaactg
     8941 cgccaagccc tgaccgcggc aggcgcaccg atggaacgca tcgacatctg ccataccacc
     9001 caagtcgtcg gcatggacga agccccgcaa tccgccctga aaaacaaaaa agactcctcc
     9061 atgcgcgtcg ccatcaacca ggttaaagaa ggcaaagccc aagccgccgt atccgcaggc
     9121 aacacgggtg cgctcatggc aaccgcacgt ttcgtcctca aaaccattcc cggcatcgaa
     9181 cgccccgcca tcgccaaatt ccttccttcc gacaccgacc acgttaccct tgcactcgac
     9241 cttggcgcga acgtcgactg cacgcccgaa cagctcgccc aatttgccgt tatcggcagc
     9301 gaactcgtcc acgcactcca tcctcaaaaa ggacagccgc gcgtcgggct ggtcaacgtc
     9361 ggcacggaag acatcaaagg tacggacacc gtcaaacaaa cctacaaact gctgcaaaac
     9421 agcaaactca actttatcgg caacatcgaa agcaacggca tcctctacgg cgaagcagat
     9481 gtcgtcgtcg ccgacggctt tgtcggcaac gtcatgctca aaaccatcga aggcgcggtc
     9541 aaattcatga gcggagccat ccgccgcgaa ttccaaagca acctgttcaa caaacttgcc
     9601 gccgttgccg ccctacccgc cctcaaaggg ctgaaaaaca aactcgatcc gcgcaaattc
     9661 aacggggcca tcctgctcgg gctgcgcggc atcgtgatta aaagccacgg cggcacagac
     9721 gaaaccggtt tccgctacgc actcgaagaa gcctaccacg aagccaagtc cgccggcctt
     9781 tccaaaatcg aacagggcgt agccgaacaa ctcgccgcac tcgaaactgc caaagccgtc
     9841 caaaacgaaa atgtcggcgg tctgtaacac gcgcgatgcc gtccgaacgc ccccgcccct
     9901 ttcagacggc atccgcccgc cccgaacctg cgggcgcgga cggcgatgca cctgtccggc
     9961 acttcccaaa tacttccctg taaaataagg aatatttgaa aaatgaagac attagaaaaa
    10021 cggatgaaag ctctagacaa acggatgatg aagttcggaa aatcccttga aggcaggctt
    10081 gatgcccgtc tgattgaatc cgcattggat tatattcatt attcggaacg ttttttggct
    10141 tttgaaatcc tgtgtactta tatcgaagat ttcgatgtcc ggctgacgga acaagaatcc
    10201 cgggaaattt cttttatcaa caaggaattt gagatagaaa gcacgtccga ttaaccaata
    10261 aagccaatgg gttgataaac atgaaaacat cgacggtcgt ttttggcgga ttttttatgg
    10321 cagacaacgg agagcgaatc caaatccccg ttttggaaaa tcctgacatt agggaaatca
    10381 atcacttttt tccgtatcaa attttgagaa aaaagccggc gtccttgttt tcagaatcat
    10441 ccccgagccg gaatttggcg ataccgaatt aactgtctat tttgaaaagg atattatagt
    10501 ggattaacaa aaaccagtac ggcgttgcct cgccttgccg tactggtttt tgttaatcca
    10561 ctatatcaga cgaaaacaaa cacccgcgcc aatagcctga cggcaacccg gcaatcaaaa
    10621 tgccgtctga agcagcttgg gctttcagac ggcatttcct ccgcttaaaa cagcatatcg
    10681 gcaaccccgc cccgcccgtc cacggcaatc tgcatctgaa aaccatctgt atgccaaacc
    10741 acacccccat cccgatttcc gtcatttgca ccctgcctgt attgggcaat catcggtttt
    10801 tcgcttacaa tagccgaatc tgaaccaact atccaaaaag gccgttccca tgcagtatgc
    10861 caaaatttcc ggcacgggca gctatcttcc tgccaaccgc gtcagcaatg acgaccttgc
    10921 ccaaaaagtg gatacctcgg acgagtggat taccgcgcgc acgggcatca aattccgcca
    10981 tattgcagcc gaaaacgaaa aaaccagcga ccttgccgcc gaagcggcgc ggcgcgcttt
    11041 ggcagatgcc aaattaaata taaacgacat tgatttgatt atcgtggcga cggcaacgcc
    11101 ggatatgcag tttccttcta ccgccaccat cgtgcagcaa aaattgggca tcaccaacgg
    11161 ctgtcccgcg tttgacgtac aggcagtgtg cgcaggcttt atgtacgcgc tgaccacggc
    11221 aaacgcctac attaaaagcg gtatggcgaa aaacgcgctg gtcatcggcg cggaaacctt
    11281 cagccgcatc gtcgattgga acgaccgcac aacctgcgta ttgttcggcg acggcgcggg
    11341 cgcggtggtt ttgagcgcgg cggacaaacc gggcatcatc cacagcaaac tcaaagccga
    11401 cggcaattat ctgaaactct taaacgtccc cgggcaaatc gcctgcggca aagtttccgg
    11461 ttcgccgtac atttcgatgg acggtcccgg cgtgttcaag tttgccgtca aaatgctgtc
    11521 caaaatcgcc gatgacgtta tcgaagaagc cggttatacc gccgctcaaa tcgactggat
    11581 cgtgccgcat caggcaaacc gccgcattat cgaatcgacc gcgaaacatt taggtttgag
    11641 tatggacaaa gtcgtcctga ccgtccaaga ccacggcaac acatccgccg catcgattcc
    11701 gctggctttg gatacgggca tccgcagcgg acaaatcaaa cgcggtcaaa acctgctgct
    11761 cgaaggcatc ggcggcggtt tcgcgtgggg cgcggtgctg ttgcaatatt gaacccgatg
    11821 ccgtctgaaa caggctttca gacggcattt cccatatcat gaagcggcag gctttcttca
    11881 aactgatggc gtgtgcggca tttctgtctg ccgtttcgct gcgcctcccc gtattgggcg
    11941 cgtgttacgc aatattgtcc ctctatgcgt ttgcacttta cggcatcgac aaacggcgtg
    12001 ccgtgcgagg aaaacgccgc attcccgaac accgcctgct gctgcctgcc ttgttcggcg
    12061 gttggacggg cgcatacttg ggcagcagga tattcaggca taaaacggcg aaaaagcgtt
    12121 ttgttgtgct gttccgtctg actgtttcgg gcaatgtcct agcgacctgc atcctgattg
    12181 attatttcgt tccgcccgaa ctttttgtaa aactcgggca acatctctga caatccaacc
    12241 caacaaagga atactatgtc ttttgccttc ttttttcccg gacaaggttc ccaaagcctc
    12301 ggtatgatga acggctttgc cggacacgcc gtcgtcaaaa acacctttgc cgaagcctcc
    12361 gccatattgg ggcaggactt gtgggcgatg attaacggca gcgatgccga aatcatcggt
    12421 caaaccgtca acacccagcc cattatgctc gccgccggcg ttgccgttta ccgcgcctat
    12481 ttggaagcgg gcggcaaaac gcctgccgcc gttgccggac acagcctcgg cgaatacacc
    12541 gcactcgttg ccgccggcgc attggatttt accgatgccg tcaaactcgt gcgcctgcgc
    12601 gccgaactga tgcagtccgc cgtaccgcaa ggcgtgggcg caatggcggc gattctcggc
    12661 ttggaagatg aacaggtaaa acaaatttgc gccgaatccg cacaggatga agtggttgaa
    12721 gccgtcaact tcaactcgcc cggacaagtc gtgattgcag gcaatgccgc cgccgtcgag
    12781 cgcgctatgc ttgccgccaa agaagccggc gccaaacgcg ccctgccgct gcccgtgtcc
    12841 gtaccttccc attgcagcct gatgaaaccc gccgccgaca aacttgccga agccctgaaa
    12901 accgttgaaa tcaagcagcc gcaaatccgc gtcatccaca acgccgacgt tgccgcctac
    12961 gatgatgtcg acaaaatcaa agacgcgctc gtccgccagc tttacagccc cgtacgctgg
    13021 acggaaaccg tcaacgccct cgtttcagac ggcattgccg aatccgccga atgcggcccg
    13081 ggcaaagtgt tggcgggctt ggcaaaacgc atcaacaaag ccgccgtgtg cagcgcgctg
    13141 accgatgccg gacaggttgc cgcctttatc gaagcgcact gacttcgttc cgcaaaaagc
    13201 agcctgccct cttcaggctg cttttcacgt ccgaacggcg gcagccccat atttacgcta
    13261 taatccatcc cgaccaaacc accgacagcg gctgccgttg cagttcccgc cccaccgata
    13321 tgatagaaaa actgactttc ggactgttta aaaaagaaga cgcgcgcagc tttatgcgcc
    13381 tgatggcata cgtccgcccc tacaaaatcc gcatcattgc cgccctgatt gccattttcg
    13441 gcgttgccgc caccgaaagc taccttgccg ccttcatcgc ccccctgatc aaccacggct
    13501 tttccgcgcc cgccgcgccg cccgagctgt ctgccgccgc cggcatcatt tccaccctgc
    13561 aaaactggcg cgaacagttt acctatatgg tttgggggac ggaaaacaaa atctggaccg
    13621 tcccgctctt cctcatcatc ctcgtcgtca tccgtggcat ctgccgcttt accagcacct
    13681 atctgatgac ttgggtctcc gtgatgacca tcagcaaaat ccgcaaagat atgtttgcca
    13741 aaatgctgac cctttcctcc cgctaccatc aggaaacgcc gtccggcacc gtactgatga
    13801 atatgctcaa cctgaccgaa cagtcggtca gcaacgccag cgacatcttc accgtcctca
    13861 cgcgcgacac gatgatcgtt accggcctga ccatcgtcct gctttacctc aactggcagc
    13921 tcagcctcat cgtcgtcctg atgttccccc tgctctccct gctctcgcgc tactaccgcg
    13981 accgtctgaa acacgtcatt tccgactcgc aaaaaagcat aggcacgatg aacaacgtga
    14041 ttgccgaaac ccatcaggga caccgcgtcg tcaagctgtt caacgggcag gcgcaggcgg
    14101 caaaccggtt cgacgcggtc aaccgcacca tcgtccgcct cagcaaaaaa atcacgcagg
    14161 caacggcggc acattccccg ttcagcgaac tgatcgcctc gatcgccctc gccgtcgtca
    14221 tcttcatcgc cctgtggcaa agccaaaacg gctacaccac catcggcgaa tttatggcat
    14281 tcatcgtcgc gatgctgcaa atgtacgccc ccatcaaaag ccttgccaac atcagcatcc
    14341 ctatgcagac gatgttcctc gccgccgacg gtgtatgtgc atttctcgac accccgcccg
    14401 aacaggacaa gggcacgctc gcaccgcagc gtgtcgaagg gcgcatcagc ttccgcaacg
    14461 tcgatgtcga ataccgttca gacggcatca aagccctcga caacttcaac ctcgacatca
    14521 gacaaggcga acgcgtcgcc ctggtcggac gttccggcag cggcaaatcc accgtcgtca
    14581 acctgctgcc ccgctttgtc gaaccgtctg ccggcaacat ctgcatagac ggtatcgaca
    14641 tcgccgacat caaactcgac tgcctgcgcg cccaattcgc cctcgtctcc caagacgtat
    14701 tcctgtttga cgacaccctg tttgaaaacg tccgatacag ccgtcccgac gcgggcgaag
    14761 ccgaagtcct gttcgccctc caaaccgcca acctgcaaag cctgattgac agctccccgc
    14821 tcggactgca ccagcccatc ggatcgaacg gcagcaactt atccggcgga cagcggcaac
    14881 gcgtcgccat tgcccgcgcc attttgaaag acgcgccgat attattattg gacgaagcca
    14941 ccagcgcatt agacaacgaa tccgaacgcc tcgtccaaca ggcgctcgaa cgcctgatgg
    15001 aaaaccgcac cggcatcatc gtcgcccacc gcctgaccac catcgaaggg gccgaccgca
    15061 tcatcgtgat ggacgacggc aaaatcatcg aacaaggcac acacgaacaa ctgatgtccc
    15121 aaaacggtta ctacacgatg ttacgcaata tctcaaacaa agatgccgcc gtccggacgg
    15181 cataaacaaa atgccgtccg aaatggtaca atcgccccga ccctttcaga cggcatcata
    15241 tccgccgacc catccgatta tcttcaatca ctgtaaaacc cattatgacc caagacaaaa
    15301 tcctcatcct tgacttcggt tcgcaagtta cccagctcat cgcccgccgc gtgcgcgaag
    15361 cccacgttta ctgcgagctg cattctttcg atatgccttt ggacgaaatc aaagccttca
    15421 accccaaagg catcatcctc tccggcggcc ccaattccgt ttacgaatcc gactatcaag
    15481 ccgataccgg tatttttgat ttgggcattc cggttttggg catctgttac ggcatgcagt
    15541 ttatggcgca ccacttgggc ggcgaagtgc agcccggcaa ccagcgcgaa ttcggttatg
    15601 cgcaagttaa aaccatagac agcgagctga cacgcggcat tcaagatggt gagccaaaca
    15661 cactcgacgt atggatgagc cacggcgaca aagtgtccaa actgcccgac ggtttcgccg
    15721 tcatcggcaa caccccgtcc tgcccgattg ccatgatgga aaacgccgaa aaacaattct
    15781 acggcatcca gttccacccc gaagttaccc acaccaaaca aggccgcgcc ctgttgaacc
    15841 gctttgtctt ggatatttgc ggcgcacaac cgggctggac gatgccgaac tacatcgaag
    15901 aagccgttgc caaaatccgc gaacaggtcg gcagcgacga agtgatttta ggtctgtccg
    15961 gcggcgtgga ctcttccgta gccgccgcgc tgattcaccg cgccatcggc gaccaactga
    16021 cctgcgtgtt cgtcgatcac ggtttgttgc gcctgaacga aagcaaaatg gtgatggata
    16081 tgttcgcccg caacttgggt gtgaaagtga tacacgtcga tgccgaaggg cagtttatgg
    16141 cgaaactcgc cggcgtaacc gaccccgaga aaaaacgcaa aatcatcggt gcggaattta
    16201 tcgaagtatt tgatgccgaa gaaaaaaaac ttaccaacgc caaatggttg gcacaaggca
    16261 cgatttaccc tgacgtaatc gaatccgcag gtgcaaaaac caaaaaagcc cacgccatca
    16321 aatcgcacca caacgtcggc ggcctgcctg aaaacatgaa gctcaaattg cttgagcctt
    16381 tgcgcgattt gttcaaagac gaagtacgcg aattgggtgt ggctttgggc ctgccgcgcg
    16441 aaatggtgta ccgtcatccg ttcccgggtc cgggtttggg cgtgcgtatt ttgggcgaag
    16501 tgaaaaaaga atatgccgac ctgcttcgtc aggcagacga tattttcatt caagaattgc
    16561 gcaatactac cgatgaaaac ggtacatctt ggtacgacct gaccagccag gcattcgccg
    16621 tgttcctgcc cgtcaaatct gtcggcgtaa tgggcgacgg ccgcacatac gattacgtca
    16681 ttgccttgcg tgccgtgatt accagcgact ttatgaccgc gcattgggcg gaactgccgt
    16741 attccttgtt gggcaaagtg tccaaccgca tcatcaacga agtcaaaggc atcaaccgcg
    16801 tggtttatga tgtgagcggc aaaccgcctg ccaccatcga gtgggaataa acagcaaaca
    16861 tggctgcccc gtccggcgca gtccttcgat tatcggaaaa aaggaaaaaa tatgagcaca
    16921 caagatttaa acggcaaaat cgctttggta acaggcgcat cgcgcggtat cggtgcagca
    16981 attgccgaca cgctggcggc agccggtgcc aaagtcatcg gtacggcgac cagtgagagc
    17041 ggtgcggcgg cgattagcga gcggttggcg caatggggcg gcgaaggccg cgtattaaat
    17101 tccgccgaac ctgaaaccat cgaaagcctg attgccgaca tcgaaaaagc gttcggcaaa
    17161 ctcgatattc tggtcaacaa cgccggcatc acccgcgaca acctcctgat gcgcatgaaa
    17221 gaagaagagt gggacgacat catgcaggtc aacctcaaat ccgtgttccg cgcttctaaa
    17281 gccgttttgc gcggtatgat gaaacaacgt tccggccgca tcatcaacat cacatccgtc
    17341 gtcggcgtga tgggcaatgc cggtcaaacc aactatgccg cggcaaaagc aggcttaatc
    17401 ggtttctcca aatccatggc gcgcgaagtc ggcagccggg gcattaccgt caactgcgtc
    17461 gcccctggct ttatcgatac cgacatgaca cgcgccctgc cggaagaaac ccgccaaacc
    17521 tttaccgccc aaaccgcctt gggcagattc ggcgacgcgc aagacatcgc cgatgcggtt
    17581 ctgttcctcg cttccgacca agcaaaatac atcaccggcc aaacgctgca cgtcaacggc
    17641 ggtatgctga tgccttaaca gacaactttt tcaaccatgc cgtctgaagc cctttcagac
    17701 ggcatttgca ttctcaggca aaatgaacac acaccacacc ccgccctgcc catgcggctc
    17761 aggcacaagc tgagaccttt gcaaaattcc tttccctccc gacagccgaa accccaacac
    17821 aggttttcag ctgttttcag ctgttttcgc cccaaatacc gcctaattct acccaaatac
    17881 ccccttaatc ctccccggac acctgataat caggcatccg ggtcaccttt taggcggcag
    17941 cgggcgcact t
//
LOCUS       DAXHNE010000025        17033 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.025, whole genome shotgun sequence.
ACCESSION   DAXHNE010000025 DAXHNE010000000
VERSION     DAXHNE010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17033
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.025"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(189..1358)
                     /gene="metK"
                     /locus_tag="ACFM4Z_000677"
     CDS             complement(189..1358)
                     /gene="metK"
                     /locus_tag="ACFM4Z_000677"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFC6020747.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGLL
                     PWLRPDAKAQLTVVYDSESGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1541..1651)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1704..2600
                     /locus_tag="ACFM4Z_000678"
     CDS             1704..2600
                     /locus_tag="ACFM4Z_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC6020748.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     TEYWIRRFPTQYLFMYNRYKAP"
     gene            complement(2644..3708)
                     /gene="tsaD"
                     /locus_tag="ACFM4Z_000679"
     CDS             complement(2644..3708)
                     /gene="tsaD"
                     /locus_tag="ACFM4Z_000679"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFC6020749.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     GKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYELLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRFEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVR"
     gene            3715..3840
                     /locus_tag="ACFM4Z_000680"
     CDS             3715..3840
                     /locus_tag="ACFM4Z_000680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020750.1"
                     /translation="MPLKTEKRILADFGTTAVSQKRNMPSEDRSDGIVAPYQSVP"
     gene            complement(3825..5012)
                     /gene="ccsB"
                     /locus_tag="ACFM4Z_000681"
     CDS             complement(3825..5012)
                     /gene="ccsB"
                     /locus_tag="ACFM4Z_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HFC6020751.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKSWLPQSVLIEEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(5005..6957)
                     /locus_tag="ACFM4Z_000682"
     CDS             complement(5005..6957)
                     /locus_tag="ACFM4Z_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HFC6020752.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTINREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILSASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQESKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7234..7857)
                     /locus_tag="ACFM4Z_000683"
     CDS             complement(7234..7857)
                     /locus_tag="ACFM4Z_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6020753.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            8063..8692
                     /gene="yihA"
                     /locus_tag="ACFM4Z_000684"
     CDS             8063..8692
                     /gene="yihA"
                     /locus_tag="ACFM4Z_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFC6020754.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(8813..11209)
                     /locus_tag="ACFM4Z_000685"
     CDS             complement(8813..11209)
                     /locus_tag="ACFM4Z_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HFC6020755.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11361..12476
                     /gene="pilM"
                     /locus_tag="ACFM4Z_000686"
     CDS             11361..12476
                     /gene="pilM"
                     /locus_tag="ACFM4Z_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFC6020756.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12479..13078
                     /locus_tag="ACFM4Z_000687"
     CDS             12479..13078
                     /locus_tag="ACFM4Z_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFC6020757.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            13079..13726
                     /locus_tag="ACFM4Z_000688"
     CDS             13079..13726
                     /locus_tag="ACFM4Z_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFC6020758.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13744..14289
                     /locus_tag="ACFM4Z_000689"
     CDS             13744..14289
                     /locus_tag="ACFM4Z_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HFC6020759.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14308..16593
                     /gene="pilQ"
                     /locus_tag="ACFM4Z_000690"
     CDS             14308..16593
                     /gene="pilQ"
                     /locus_tag="ACFM4Z_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HFC6020760.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSEPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQAAAPAKQQAAAPAKQT
                     NIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKT
                     PVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKT
                     FTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
                     ARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRS
                     ILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQV
                     MIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINL
                     PITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIP
                     FTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTIL
                     CISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDR
                     RELLIFITPRIMGTAGNSLRY"
BASE COUNT         4596 a         4829 c         3880 g         3728 t
ORIGIN      
        1 cggaagaagg tactcatgga aaatccccta aatgtcttgg tgggaattta ggggattttg
       61 gggaattttg caaaggtctc tatctgtact gtctgcggct tcgtcgcctt gtcctgattt
      121 ttgttaatcc actatataaa aatgccgtct gaactgttca gacggcattt ttgattttca
      181 aaccggaatt acagccccgc tgccgccctc aatgcagcag ctttgtcggt gcgctcccaa
      241 gtgaactcag gttcttcgcg gccgaaatgt ccgtaagcgg cggatttact gtaaatcggg
      301 cgcaagagat cgagcatttg gacgatgcct ttggggcgca ggtcgaaatg ttcgcgaact
      361 aaggtaatca gtttttcctc gctgattttg cctgtgccga aagtgtcgat ggaaatcgaa
      421 gtcggttcgg caacgccgat ggcgtaggaa acttggattt ggcattgggt tgccaaaccg
      481 gcggcgacga tgtttttcgc aacgtagcgg caggcgtaag cggcggaacg atccactttg
      541 gacgggtctt tgccggagaa cgcgccgccg ccgtgcggag ccgcgccgcc gtaggtatca
      601 acgatgattt tacggccggt caaaccgcag tcgccttgcg ggccgccgat gacgaagcgg
      661 ccggtcgggt tgatcaggta tttggtttcg tcggtcagca gttcagacgg caggacgggc
      721 ttgataatct gctcaatgac ggcgtttttc aactcttcat aaccgatttc cgggtcgtgt
      781 tgcgtagaga ggacgacggt atcaatgcgt tttactttgc cggattcgct atcgtaaacc
      841 acggtcagtt gggcttttgc gtcgggacgc agccaaggca ggaggccgtc tttgcgtaat
      901 tcgctttgac gctgcatcag gcggtggctg taatagatgg caaacggcat cagggtcggg
      961 gtttcgtcgc aggcatagcc gaacatcaaa ccttggtcgc cagcgccttg gttcaagtca
     1021 atgccttcgc cttcgttcac gccttgggcg atgtccggag attgctggtc gtagtacacg
     1081 ccgaccgcgc agccgttggc atcaaagccc agctcggagg agttgtagcc gatgcgtttg
     1141 atggtttcgc gtgcgacttt gatgtagtcc acttgggcgg tggtggtgat ttcgcctgcc
     1201 aatacgcaca agcctgtgtt gaccaaagtt tctgcggcga cacgcgcttt ggggtcttgc
     1261 gccaagatgg catccaaaat cgcgtcggat acttggtcgg caactttgtc cggatggcct
     1321 tcggataccg attcggaagt aaacagatat tcgctcattg agattttcct tgaaaaacaa
     1381 accatcttct tcagacggca tgttgtatga acataatgtc gacagcggga aatatagcaa
     1441 aatttcccta ttcataccat tcagttgaga aatattccca tttgaatggc actttggaat
     1501 ctctgcccgt acgtttctta caggcaaaaa aattcccgca tcaagcgggt ttggattgct
     1561 ctggtgagcc acatcggctt ttcaaccgtc caccttactt ttccttttga aaagcaggtt
     1621 ggcatggaat tcccaactct taatgcagcg cggatggtag cagaaaagcc tttccgccgc
     1681 aatacttccc tttttcagac ggcatgtttc gtttacaatt cgggctgttt ccccctttgc
     1741 gaaccgccat gcacatcctg ttgaccgccc tgctcaaatg cctctccctg ctgccacttt
     1801 cctgtctgca cacgctggga aaccggctcg gacatctggc gttttacctt ttaaaggaag
     1861 accgcgcgcg catcgtcgcc aatatgcgtc aggcaggcat gaatcccgac cccaaaacag
     1921 tcaaagccgt ttttgcggaa acggcaaaag gcggtttgga acttgccccc gcgtttttca
     1981 gaaaaccgga agacatagaa acaatgttca aagcggtaca cggctgggaa catgtgcagc
     2041 aggctttgga caaacacgaa gggctgctat tcatcacgcc gcacatcggc agctacgatt
     2101 tgggcggacg ctacatcagc cagcagcttc cgttcccgct gaccgccatg tacaaaccgc
     2161 cgaaaatcaa agcgatagac aaaatcatgc aggcgggcag ggttcgcggc aaaggaaaaa
     2221 ccgcgcctac cagcatacaa ggggtcaaac aaatcatcaa agccctgcgt tcgggcgaag
     2281 caaccatcgt cctgcccgac cacgtcccct cccctcaaga aggcggggaa ggcgtatggg
     2341 tggatttctt cggcaaacct gcctatacca tgacgctggc ggcaaaattg gcacacgtca
     2401 aaggcgtgaa aaccctgttt ttctgctgcg aacgcctgcc tggcggacaa ggtttcgatt
     2461 tgcacatccg ccccgtccaa ggggaattga acggcgacaa agcccatgat gccgccgtat
     2521 tcaaccgcaa taccgaatat tggatacgcc gctttcctac ccagtatctg tttatgtaca
     2581 accgctataa agcaccgtaa cgaaaataaa aatgccgtcc gaacaacttc agacggcatt
     2641 ttgtcatctg acgatttccg acagcggcca gcgcggacgg acattgaacg caccgacctc
     2701 cctgcccttg cccaggtgca tcgcaccggc aaaggcaatc atggcaccgt tgtccgtgca
     2761 gtatgccgtc ggcgggaaaa acacgctgac tttttcggac ggatgtttcg gcttgccttt
     2821 gggggtcggg atttgcaccg tcatgttgcc gaaagtttca cggagcttgc ggtttgcacc
     2881 gaccccgccg gcgaccacta cggttctgaa ccctgtctgc aacagggctt ttttcacttt
     2941 cgccgccaac acatcgacta ccgcatcttg aaacgcacgg cagatgtcgt tgcgtgtctg
     3001 ctcaggaatg tcatccgccc cgttttccgc gcgcactttc tcgacggcgg tcaatacggc
     3061 ggttttcaaa cctgaaaaac tcatctgcaa atcgtcggaa tgaatcatcg ggcgcggaaa
     3121 aacaaacgct tcgaacctgc ccgattccgc aagttccgac agtttcgcac cgcccggata
     3181 cggcaagccc agcagtttcg ccgttttgtc gaatgcctcg cccgccgcat catcgacgct
     3241 ctcgcccaaa agctcgtagt cgcctatgcc cctgaccgcc ataatctgcg tatgcccgcc
     3301 cgaaaccaac agcgcgacaa aaggaaagtc gggtttttcc tccgccaaca gcggcgacag
     3361 cagatgtcct tccaaatgat ggacggggat gacgggcttg cccaatgcca aagccagtgc
     3421 attggcgtag ctcgaacccg ccagcagcgc accgcccaaa ccaggcccct gcgtaaaggc
     3481 aaccgcgtca atgtcgccat acgatgcgcc tgcctgcgcc aaacagcctt cagtcaacgg
     3541 aacaaggcgg cggatatggt cgcggcttgc caattccggc acaaccccgc cgtattcggc
     3601 gtgcattgcc atttgagtgt gcaggcagtg cgcccgcaat ccacgttccg tatcgtaaag
     3661 cgcaacacct gtttcgtcgc aagaagactc gattcctaat accaacatgg tctgatgccg
     3721 ttaaaaactg aaaaacgtat tttagcggat ttcggcacga ctgccgtatc ccaaaaacgg
     3781 aacatgccgt ctgaagaccg ttcagacggc atcgtcgcac cgtatcaaag cgttccgtaa
     3841 gaatgcagcc cgctcaaaaa catattcacg ccgataaagg cgaatgcggt tacgaacaaa
     3901 ccgataatcg cccaccacgc cagcactttg ccgcgccaac cggcaaccag ccgcaaatgc
     3961 agccacacgg cataattcaa ccaaacgata aacgcccacg tctctttcgg gtcccaactc
     4021 caatagcgtc cccaagcatc cgccgcccac agcgcaccca aaatggtggc aatggtaaag
     4081 aacagaaagc cgacggcaat cgccttatac ataacttcct caatcaacac ggattgcggc
     4141 agccacgatt tcccgccttt tccttccgca cgcagggaaa ccagttcggc aataccgagc
     4201 atcgcggaaa tgcaaaacgc gccgtaaccg ataaagtttg ccggaacgtg gattttcatc
     4261 caccaagatt gcaatgccgg aatcagcggc tggatggtat gcgcctcgcg ggacacgctg
     4321 taccacaaga caaatccaac cacgaccgcc ataaagccga acacgaagcc gcccaatttc
     4381 tgtatggcga acttaccttc ataataaaga tacatcagcg cggtaatcac caaaaacagg
     4441 atgaacactt catacaggtt ggaaaccgga atatgccccg catcgggacg gagcagatag
     4501 ctttcgtgcc aacgtaccag cagaccggta aagcctgcta cggcagacac ccatgcaaac
     4561 acggttccca tacccaacag cgtgttggtc ggcacatttt ttacgcttgc caaaaccgcg
     4621 cccgaaatat aggcgaacag ggcgaagaag acaaaggcgc actgccacat aatcgccgac
     4681 tggctgctga ggaaataccg caacaggaaa atctctgccg atttgatgtt gccgccatac
     4741 agaccgacgg cggcataggc aagcaatacg cttaaaggaa caaaccagcg catcggtttg
     4801 aaaaaccaac ccaaaaacac ggcaataccg gcactcgccc acaacatgac cgtttcgtaa
     4861 atgtccatat gcataccgga acgggtctgt acgaaaaccg tagccgcaaa aaccagcacg
     4921 gcaaataccc aatcccaaag attcagattg ctgatcaaag acttctgaat cagcagctcg
     4981 tgttccggaa gggttttata gtgttcagtc atgattcaag tccttgccga gccgttgcag
     5041 actctcgacg tgttttggaa attccttctg caaatcccgt tcactgcggg ccgaagacat
     5101 ggcaaaacgg attttgccgt ctgaaaacaa tacccacgcc cgtttttcgc gcacataaaa
     5161 catcaatacc gtacccaata ccaacagcac cgagccgaga tagaccaaaa gcgcacccgg
     5221 ggaacgggtc atctgcaaac ccgacgaacg cacctcggaa aacccgtcaa gttgcagcag
     5281 cataggcgcg ggatattcgg tcaaacccgt gtacgcatcc atactgtgca gcaggaaacg
     5341 attccgcgct tcatcctgct gccattcggg caagccgtac cggcgtatgg tttcatccaa
     5401 agcagcgttc atcacgccgt aaagcatttc gtagaaatag ccctgcatct tatcctgctg
     5461 ctctttcggg atattggacg taataaattc gtccaatccc aaatagcctt tttgtgcaaa
     5521 gatgttcagc gtgttttccg cagccagcat gaattgttcg cggatttcgg caggtgcgtc
     5581 tttggttgcg tcggcaacca gacgtttgcg cccttcccca tctttcaaaa actcacgcaa
     5641 tgccataaag gtgtccgctt tcaactgctt gtccaagggg atacgcagcc agcggtattg
     5701 ctgctgcaag ccgctgcgcg tgccggtaat ccaaaaataa tcctgttcct gcaaaaccgg
     5761 cagcatatag tttttatatt cgaccgcctg ccctgccgca tcacggatac ggtaaacaat
     5821 ggaagggccg atattggtgt attttttact ttcctgagta acggcgcgga catcgttcag
     5881 cgtggatttc aggctttttt cccgttccgc gccctcgctc atgtcctcca cattcataga
     5941 agtgaactga tcgaactcaa gacgatattt gtgtttgcca atttccaacg gaaactggtg
     6001 tatggatgtt gccttcaaca cgacaggctc gcgcgaagca tcacccaaat tccacgcctt
     6061 gaatgtcaaa tccgaaccgc cgtcggcaaa actcgcctga taaatcgtga tgccgtgcaa
     6121 ggtcaaagga tggttcacgc ggatggtgcg ctcgagtttc tcaccggttg ccttgtccgt
     6181 cacttcaata tcgctggcga aatcacgcgg catacccgta ttgtaaaaat cgatatggaa
     6241 ttttttcagt ttgacttcaa aaggcaagtc ctgaaccaat atcccgttgt cggcattcag
     6301 gaaaaccaca tccgcactct gcccctcgga aatattgacg ttgcccctaa atgagagatt
     6361 ggacgcactc aaaatacttt cgggcttgaa atccttggca taaaccgcct gattgtccgg
     6421 aacaatccga ccggtcagca tacccagttt caacagcagg ttactgtcta tcaacccgcc
     6481 caggcaaatg acaatcaaag caacatgggc aaagatatag ccccatttgt tcattgtgcc
     6541 ttttttggcg gcaatcagaa ccgacccgtc ttcacggtta atggtttttc cctgaaaacc
     6601 ttgtacttcc agataacgtt tggcaacctc gggcgcaatt tttacatcca acagcgaaga
     6661 atggcgcatc gccgccagag atttttcttt aaccttttcc cgaaaagact tcatttcgcg
     6721 ccagaacggc ggcacattgc gaatcaggca caaactggta gaaaccacca aaaacatcat
     6781 gataacgaca aaccatgccg aagcatagac gtcatacagt cccagaaaac caaaaatctg
     6841 cgcccaaaaa ggtccgaatt tgaccaaata atccgtctgc ggctggtttt gctgcaacac
     6901 cgtaccgata accgatgcaa tacccagcag actgagcaaa gcgactgcaa agcgcatgga
     6961 gctgaaaaaa gcgaaccacg gacgggaaag aagtggggga gatctacggg atttactcat
     7021 tgtgtgttta ttccgccatc aggaatatgg gaaagcagaa ttgggcaaac agaaaacaac
     7081 gtcccgattc tactgtcttg atgcttttta tttcaagaca atgaagacag cctgcatcga
     7141 ttccaacggt tgcgattgaa aaaacttatc gcagaattgc ctgaagccgt ctgaaaactt
     7201 ttcagacggc ctctaaaaca gactattgcg gaattaacgc aaaccttgga taaagttggc
     7261 gaccgctttc aaatcttctt cagacatacg gtttgcaata tcttccatga tggtattttt
     7321 acgctgaccg gacttgtagg cattcatctg ttcaacaata tatgcctgat gctgaccgcc
     7381 caaacgcgga taagcctgaa tttcgcttcc gcctcccggc atacccgcac cgctcggacc
     7441 gtggcaggac atacacgccg gcactttttt atcgctcaaa ccgccgcgat agattttcgc
     7501 acccaattcg ggattttcct taggattggc ttcaccggat ttgggctgct gtttggcata
     7561 gaatgcggat acgttcaaaa tatcctgatc gctcaaattc attaccaccg gtttcatcac
     7621 agctgccgaa ccgtgggtgc gtttaccgtc gcggatgccg atagtttgat gatagatgta
     7681 agcagtatgc tgtgccgcca aacgcggata catcgcaatg ccgctgttac cgtctgctgc
     7741 atggcaagcc gcacaaaccg ttgcggcaac ctgtttgcct ttttccacgt ctgctttggg
     7801 agaggcggaa accgcaccgg cagccaaaac aaaggccaat aaagtcaatc gtttcatgga
     7861 gtgctcctga ttacagcatt ggataacgca acaatgctct ttttatattc aaatacggga
     7921 tttttgaccc gattaaaacc gatgattctg taaacgtgtt attctatact aaatttacat
     7981 taaattacca ctgtgtttca cataaaacca accgcatatt tttgctgtcg gacaaacggc
     8041 ggcggaaaac aaggatatgc ccatgaacct ttttcaaaac gccaaattct tcacgacggt
     8101 caaccacctt aaagacctgc ccgacacccc tctcgaaatt gcctttgtcg gcaggagcaa
     8161 tgccggaaaa tccagtgcca tcaataccct gaccaaccat gtccgtcttg cctacgtttc
     8221 aaaaacaccc ggacggacgc agcatatcaa cttcttcgag ctgcagaacg gcaattttat
     8281 ggtcgatttg cccggctacg gttatgccca agtccctgaa gcagtacgcg cacattgggt
     8341 caatctgctc ggcgactatc tgcaacagcg caaacagctt atcgggctgg ttttgattat
     8401 ggatgcccgc catcctttaa aagaactcga catccgtatg ctggattttt tccacacgac
     8461 cggcagaccg gttcacatcc tgctgtcaaa agccgacaaa ttatccaaaa acgaacagat
     8521 aaaaaccctg tcccaagtca aaaaactgct caaaccttat tccgacaggc aaaacatcag
     8581 cgtacagctg ttttccagcc tgaaaaaaca aggtattgac gaggccaacc gaactgtcgg
     8641 aagctggttt gacgcagcag atgctgccgc ttcctctcca gaggaaaact gaccccaatt
     8701 atacggaaac cgtatttccc ccacttgacc gaccgcaaac atttaaaaaa ttgccactgc
     8761 caaatctaaa atgccgtctg aaagactttt cagacggcat tttgcggagt ctttaaaaca
     8821 gagaatccaa ctgctgctgt ttggaaccag tattactcgg aagcaccggc gtttcctgca
     8881 tatcttggcg gacttcgtca tccgccgcct gccgtccgcc ttctgccgcg cccccgtcat
     8941 cttcttttgc ccgtcgggaa ggttgcggcg caataccgct gttgtccagc gtcaagcccg
     9001 gatcggttac catacgttcc ttcatatagt attcgccatt gctgctgacc acaccttcag
     9061 gcattttcat ccccttgccc tgctttcctt tcaacgcaaa acgcatatag tccacccaaa
     9121 ccggcaccgc aatcgtaccg ccgtagccga cacgccccat actcttaggt ttgtcgaagc
     9181 cgatatatac ggcagtaacc acatcagggt taaaaccgac aaaccacgca tccttattgt
     9241 cattggtcgt acccgtttta ccggcaatat ccgttcttcc caacgcagct gccccccttg
     9301 ccgtaccgac acggaccaca tcctgcataa tcttatacat aatataggca ttgcgcggat
     9361 cgattgcctg aggcgcattt tgcccagcca ccaaaggttg catttgggcg cgcaacctgc
     9421 cgtctctgtc ataaatctta tcgattacgt gcgaagaaac cctatatccg ccgttcgcaa
     9481 atacgctata tgcctccgcc actttcaacg gcgttgtctc gcccgtacct aaagccatag
     9541 acaggcttgc cggcagctcg gacgacctga agccgaaacg ccggatatac tgttgcgcgt
     9601 aaccgacacc gatagacatc aaaatacgga tggaaaccat attcttggaa gccgtcagag
     9661 cctgtctcaa agtaatgtag ccggaatatc tgccgtctga atttttaggt gtccaaaccg
     9721 aaccgttcgg ccctttcccc ggcagggaaa tcggcgcatc gttaaccact gtggacgcgg
     9781 tcatcccctt agataatgcc gccgaataga caaacggctt aaaggtcgaa cccggctgcc
     9841 gcattgcctg aacggcacga ttgaatgttt tgctgtgaaa atcataaccg ccgaccagcg
     9901 cgcgcacagc tccggttttt gcatccagcg aaaccaaagc cccctgcagc aacggctctt
     9961 gaaccaccgc ccaacgcccg ccgttgtttt tgacacggat gaccgcgccc ctgcggatac
    10021 ggtcctcccc cattttttca ttattgaccg cgcgggccgc aaagcccaag gcgcgcctat
    10081 caagcgtaac ccgcctgccg ccgggcagct gtatgacgac atttttcttt ttagtcacat
    10141 ccaacacaac ggcgggaacc attttatcga cggtatagag tcccgacaga tactggctga
    10201 cagtctcctc gacatcttca ctcttactca aatcgatata gttttccgca ccgcggtagc
    10261 tgctgccgcg atcgaaattc cgtagagcct tgcgcaatgc ctcggttgcc accttctgat
    10321 gatcggcgcg gaccgtggta taaaccttaa aaccctgcgt ataggcatct tcaccgtatt
    10381 tctcatacag ttcctgacgc accatttccg ccacatataa agcactctga tcgattttcc
    10441 gaacaaaccg ctcgtaatgc agttcctcat tcaacgcctg atcgcgctgt tgcacggtaa
    10501 tcatcttctc ctcgagcata ttgttcaaaa tatacttctg gcgcaacttg gcacgttctg
    10561 gattaacaat cggattatag gcagacggag ccttgggcag tcccgcaagc atggcggctt
    10621 ccgccaaagt caaatctcgg acattcttat tgaaatagat ttgcgcggca gatgcaaaac
    10681 cataggcgcg ctgaccgagg taaatctgat tgaaatacaa ctcgaggatt ttgtctttgc
    10741 ttaaagactg ctcgatttta taggcaagca acacctcatt gaatttgcgt gtgaacgttt
    10801 tttcactgct caaataaaaa tttttcgcca cctgctgcgt aatcgtactc gcacccgact
    10861 gcacgctgcc ggacacgaca ttgccgacgg cagcgcgggc aacaccccaa acatccaccc
    10921 cccaatgccg gtaaaagcgt ttgtcctcgg cggcgataac cgcattccgc aacacctctg
    10981 ggaaatcgcc gatttttgta aattcgcgcc gctgctcccc atacataccg atgacttccc
    11041 catccgccga ataaatagtc aacggcattt taggctggta atgctgcaaa gaatccaaag
    11101 acggcagttt cggatacgtt accaaaatag caatggcaac caaacccact ccaaatacac
    11161 aaaacccaaa aaccaaacca aaacaagtcg ttaaaatctt tttaatcata gctgaataat
    11221 aatttaccat tattggtatt aaataaagta aaatagcaac cgatttctac aaagcacggt
    11281 ttcaatgtgc aaagaacaag gaatccatta cggataccga aacggttact cactgtacaa
    11341 ataaagcagg aactttcatc atgcgcttgt ttaaaagctt gaaaaaccct aaaaaaacag
    11401 atgccaagct ccctaaaaaa tcttcgggac tcaataaccg cgcggcaatc ggcatcgata
    11461 tcgaccagca ttccatcaaa atggtccaat tgtcaggacg tagtttaaac caaattcaat
    11521 tggaaaaata cgtcattgcc aaattaccaa agaatatcat tcaaggcaat aaagtccaaa
    11581 attacgatca acttgttaca tatttgcaac aagcctatgc caaactgggt acttcgtgca
    11641 aaaacatcat cgcgtccgtc ccgcaaaatc tggcaaccat cgaacaattg acctacacag
    11701 acaaagatgc agaattagac ctgcaggggt tcgtggagtc ctccatctcc gaagtcagct
    11761 cgatatcgct cgaagaagcc aattacgact atcaggtctt gtcccaatcg gccgccggcg
    11821 aagctgtgtt ggccgtcgca tcgagaaagg atgaaatcga acccctgatt gacgcattca
    11881 acgccgccgg tatgaaatta tccgcgcttg atgtggacat tttcggacaa tacaacgcct
    11941 acgcgctatg gataaaccat ttcgcccccg agcttgcagc cgaaaaagtc gccattttcg
    12001 gcgtatatgc cgcacagacc tacgccttgg tcatccaaga cggaaaaatc ctatacaaac
    12061 aggaaacctc cgtcagcgaa gaacagctca accaactcat ccagcgcacc tatcaggtaa
    12121 cagaagaaaa agcggaagaa atcatcaact ccccgcaaaa accttccgat taccaagaaa
    12181 gcgtggcaaa ctatttcaac cagcagatta cccaagaaat acaaagggtc ttgcagtttt
    12241 attacaccac gcagaccgca gacgatatga ccgacatcaa gcatatcctg ctgaccgggg
    12301 aagcggcgcg ccaggaaggc atcgcccaaa ccgtcgcctc acaaaccaat gcagatgtac
    12361 aatgcgtcca tcccgcgcgt tattttgcgg acaacctcaa aacagacaaa caacaattcg
    12421 aacttgatgc gccgacactg accagggcgt tcggtttggc ggtacgggga ttataattat
    12481 gaacaattta atcaaaatca acctcctccc ctacagggaa gagatgaaca agcgcaaaca
    12541 gcagcagttt aaaacgctga tgtacggtgc cgtgctgacg ggcgttgccg ccgttgcggc
    12601 aacctacctg tttatcgaca atatgatcaa taaccagtcg gaaagaaaca cgctgctgga
    12661 aacctccatc gcacacttgg ataccgagct gtcggaaata caaaagctca aacaggaaaa
    12721 agatgccttc ctgattaaga aaaacaaaat cgaggagctc cagctcaaac gcctccaagc
    12781 cgcaaaaatc ctcgacagcc tgaatgaggc cgtccccgga agcacctacc tgacctcgct
    12841 ggatgccgtt accgccgact cttatcggct cagcggcagg acatccagcg acaaccgcgt
    12901 tgccgccatg atgagggcga tgcccaatac cggcatattc aagcaacccg aattgttaag
    12961 catcaagaaa aacaattcgc atcaagaatt tacccttcag gcaacattac aacccatcgt
    13021 aaaggcggcc gaatccaaag agaatccggc ttcgggaaac gcacaggagg caaactgaat
    13081 ggcttctaaa tcatctaaaa ccaacttgga tctcaacaac cttcacctgc tcaaccttcc
    13141 tgccaggctt tttatcgccc tgctggccgt tgccgccgtg ctggggctcg gttatgccgg
    13201 attgttcaaa agccagatgg aatcccttga ggaatacgaa gcaaaagaaa ccgaactgaa
    13261 aaacacctac aaacagaaaa gtatcgacgc ggccagcctg aacaacctga gggacgaact
    13321 tgcctcaatc cgctctgcct tcgatatcat gttgaaacag ctgccgacag atgcagaaat
    13381 tcccaacctg gttcaagagc ttcatcaggc aggttcgagc aacggcctgc gcttggacag
    13441 cgttatgccc caacctcccg tagatgacgg ccccatcaaa agattaccct attccatttc
    13501 cattaccgga aattacgaac agatcagcca atttacccgc gatgtcggca gcctctcccg
    13561 aatcattacc cttgagtcgc tgaaaatcgc ccaatctccg gaaaacggcg gcaatcctga
    13621 cggcaagagc agcatcctga acctcagcgc cattgccacc acctaccaag caaaatccgt
    13681 agaagagctt gccgcagaag cggcacaaaa tgccgagcaa aaataactta cgttagggaa
    13741 accatgaaac actatgcctt actcatcagc tttttggctc tctccgcgtg ttcccaaggt
    13801 tctgaggacc taaacgaatg gatggcacaa acgcgacgcg aagccaaagc agaaatcata
    13861 cctttccaag cacctaccct gccggttgcg ccggtataca gcccgccgca gcttacaggg
    13921 ccgaacgcat tcgacttccg ccgcatggaa accgacaaaa aaggggaaaa tgcccccgac
    13981 accaagcgta ttaaagaaac gctggaaaaa ttcagtttgg aaaatatgcg ttatgtcggc
    14041 attttgaagt ccggacagaa agtctccggc ttcatcgagg ctgaaggtta tgtctacact
    14101 gtcggtgtcg gcaactattt gggacaaaac tacggtagaa tcgaaagcat taccgacgac
    14161 agcatcgtcc tgaacgagct gatagaagac agcacgggca actgggtttc ccgtaaagca
    14221 gaactgctgt tgaattcttc cgacaaaaac accgaacaag cggcagcacc tgccgcagaa
    14281 caaaattaag aagaggatta ctccattatg aataccaaac tgacaaaaat catttctggt
    14341 ctctttgtcg caaccgccgc ctttcagaca gcatcggcag gaaacattac agacatcaaa
    14401 gtttcctccc tgcccaacaa acagaaaatc gtcaaagtca gctttgacaa agagattgtc
    14461 aacccgaccg gcttcgtaac ctcctcaccg gcccgcatcg ccttggactt tgaacaaacc
    14521 ggcatttcca tggatcaaca ggtactcgaa tatgccgatc ctctgttgag caaaatcagt
    14581 gccgcacaaa acagcagccg tgcgcgtctg gttctgaatc tgaacaaacc gggccaatac
    14641 aataccgaag tacgcgggaa caaagtttgg atattcatta acgaatcgga cgataccgtg
    14701 tccgcccccg cacgccccgc cgtaaaagcc gcgcctgccg caccggcaaa acaacaggct
    14761 gccgcaccgt ctaccaagtc cgcagtatcc gtatccgaac cctttacccc ggcaaaacaa
    14821 caggctgccg caccgtttac cgagtccgta gtatccgtat ccgcaccgtt cagcccggca
    14881 aaacaacagg cggcggcatc agcaaaacaa caggcggcag caccagcaaa acaacaggcg
    14941 gcggcaccgg caaaacaaac caatatcgat ttccgcaaag acggcaaaaa tgccggcatt
    15001 atcgaattgg cggcattggg ctttgccggg cagcccgaca tcagccaaca gcacgaccac
    15061 atcatcgtta cgctgaaaaa ccataccctg ccgaccacgc tccaacgcag tttggatgtg
    15121 gcagacttta aaacaccggt tcaaaaggtt acgctgaaac gcctcaataa cgacacccag
    15181 ctgattatca caacagccgg caactgggaa ctcgtcaaca aatccgccgc gcccggatac
    15241 tttaccttcc aagtcctgcc gaaaaaacaa aacctcgagt caggcggcgt gaacaatgcg
    15301 cccaaaacct tcacaggccg gaaaatctcc cttgacttcc aagatgtcga aatccgcacc
    15361 atcctgcaga ttttggcaaa agaatccggg atgaacattg ttgccagcga ctccgtcaac
    15421 ggcaaaatga ccctctccct caaagacgta ccttgggatc aggctttgga tttggttatg
    15481 caggcacgca acctcgatat gcgccaacaa gggaacatcg tcaacatcgc gccccgcgac
    15541 gagctgcttg ccaaagacaa agccttctta caggcggaaa aagacattgc cgatctgggc
    15601 gcgctgtatt cacaaaactt ccaattgaaa tacaaaaatg tggaagaatt ccgcagcatc
    15661 ctgcgtttgg acaatgccga cacaaccgga aaccgcaata cgcttgtcag cggcaggggc
    15721 agcgtgctga tcgatcccgc caccaatacc ctgattgtta ccgatacccg cagcgtcatc
    15781 gaaaaattcc gcaaactgat tgacgaattg gacgtacccg cgcaacaagt gatgattgag
    15841 gcgcgtatcg tcgaagcggc agacggcttc tcgcgcgatt tgggcgttaa attcggcgcg
    15901 acaggcaaga aaaagctgaa aaatgataca agcgcattcg gctggggggt aaactccggc
    15961 ttcggcggcg acgataaatg gggggccgaa accaaaatca acctgccgat taccgctgcc
    16021 gcaaacagca tttcgctggt gcgcgcgatt tcctccggtg ccttgaattt ggaattgtcc
    16081 gcatccgaat cgctttcaaa aaccaaaacg cttgccaatc cgcgcgtgct gacccaaaac
    16141 cgcaaagagg ccaaaatcga atccggttac gaaattcctt tcaccgtaac ctcaatcgcg
    16201 aacggcggca gcagcacgaa cacggaactc aaaaaagccg tcttggggct gaccgttacg
    16261 ccgaacatca cgcccgacgg ccaaatcatt atgaccgtca aaatcaacaa ggactcgcct
    16321 gcgcaatgtg cctccggtaa tcagacgatc ctgtgtattt cgaccaaaaa cctgaatacg
    16381 caggctatgg ttgaaaacgg cggcacattg attgtcggcg gtatttatga agaagacaac
    16441 ggcaatacgc tgaccaaagt ccccctgttg ggcgacatcc ccgttatcgg caacctcttt
    16501 aaaacacgcg ggaaaaaaac cgaccgccgc gaactgctga ttttcattac cccgaggatt
    16561 atgggtacgg ccggcaacag cctgcgctat tgatgcgtca aaataagggc atatgtttta
    16621 cggcatatgc cctttcttta tgctttttgc cgcgaccgaa atgccgtcat tcccgcgcag
    16681 gcgggaatcc agtccgttca gtttcggtca gtttcggtca tttccgataa attcctgttg
    16741 cttttcattt ctggattccc acttttgtgg gaatgacggc ggaaggggta aatcctcaca
    16801 acccaaagcc tcgtcatttc cacaaaaaac agcaacccga aacagcaact taaaaccccg
    16861 tcattcccgc gcaggcggga atctaggtct gtcggttcag gaacttatcg gataaaacgg
    16921 tttctccaac cctgcgttct agattcccac tttcgtggga atgacgggat atgggtttcc
    16981 gtgcggacgt gttcggattt ccgcctgcgc gggaatgacg gcgacagatg ccc
//
LOCUS       DAXHNE010000026        16937 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.026, whole genome shotgun sequence.
ACCESSION   DAXHNE010000026 DAXHNE010000000
VERSION     DAXHNE010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16937
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.026"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(49..714)
                     /locus_tag="ACFM4Z_000691"
     CDS             complement(49..714)
                     /locus_tag="ACFM4Z_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6020761.1"
                     /translation="MAVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYAEMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(914..1687)
                     /gene="folE2"
                     /locus_tag="ACFM4Z_000692"
     CDS             complement(914..1687)
                     /gene="folE2"
                     /locus_tag="ACFM4Z_000692"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HFC6020762.1"
                     /translation="MNTIADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTVAR
                     LTMTVYLPAEQKGTHMSRFVALMEQHSEALDFAQLHRLTAEMVALLDSRAGKISVSFP
                     FFRKKTAPVSGIRSLLDYDVCLTGEMKDGAYGYSMKVMIPVTSLCPCSKEISQYGAHN
                     QRSHVTVSLTADAEVGIEEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDM
                     VRDVATSLIADKRIKSFVVESENFESIHNHSAYAYIAYP"
     gene            1897..3054
                     /locus_tag="ACFM4Z_000693"
     CDS             1897..3054
                     /locus_tag="ACFM4Z_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HFC6020763.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFTLHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLQPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKALAA
                     KAKATGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAIAGPMDCWLVLRGIKTLALRMEAHCKNALEIARRLEDHPAIEKVFH
                     PGLPSHEHYELAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(3117..4118)
                     /gene="hemB"
                     /locus_tag="ACFM4Z_000694"
     CDS             complement(3117..4118)
                     /gene="hemB"
                     /locus_tag="ACFM4Z_000694"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFC6020764.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAEEAYNPE
                     GLVPAAVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALNEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(4301..4585)
                     /gene="yhbY"
                     /locus_tag="ACFM4Z_000695"
     CDS             complement(4301..4585)
                     /gene="yhbY"
                     /locus_tag="ACFM4Z_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFC6020765.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            4695..5315
                     /locus_tag="ACFM4Z_000696"
     CDS             4695..5315
                     /locus_tag="ACFM4Z_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HFC6020766.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINDKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLAGPSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSLYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            5379..7346
                     /gene="ftsH"
                     /locus_tag="ACFM4Z_000697"
     CDS             5379..7346
                     /gene="ftsH"
                     /locus_tag="ACFM4Z_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFC6020767.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADTAEDN
                     APHAPAREETEAPAPADTASTESEQQPENKA"
     gene            7567..7998
                     /locus_tag="ACFM4Z_000698"
     CDS             7567..7998
                     /locus_tag="ACFM4Z_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HFC6020768.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            7991..8269
                     /locus_tag="ACFM4Z_000699"
     CDS             7991..8269
                     /locus_tag="ACFM4Z_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFC6020769.1"
                     /translation="MLEIEIVYGLSDKQVLKNMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            8322..8900
                     /gene="pth"
                     /locus_tag="ACFM4Z_000700"
     CDS             8322..8900
                     /gene="pth"
                     /locus_tag="ACFM4Z_000700"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFC6020770.1"
                     /translation="MSNKIKMLVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPTTFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            8961..9167
                     /locus_tag="ACFM4Z_000701"
     CDS             8961..9167
                     /locus_tag="ACFM4Z_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HFC6020771.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            9164..9526
                     /locus_tag="ACFM4Z_000702"
     CDS             9164..9526
                     /locus_tag="ACFM4Z_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFC6020772.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            9757..11172
                     /locus_tag="ACFM4Z_000703"
     CDS             9757..11172
                     /locus_tag="ACFM4Z_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFC6020773.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDQEKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            11291..11800
                     /locus_tag="ACFM4Z_000704"
     CDS             11291..11800
                     /locus_tag="ACFM4Z_000704"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6020774.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            11963..13345
                     /locus_tag="ACFM4Z_000705"
     CDS             11963..13345
                     /locus_tag="ACFM4Z_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFC6020775.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGFTDSGIDVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIIEKDGFVAADKQGNVTEKDISGEYHNHI
                     VGHIKLKRPMKIAIDAGNGVGGAFAGKLYKGLGNEVTELFCDVDGNFPNHHPDPSKPK
                     NLQDLIAALKNGDAGIGLAFDGDADRLGLVTKDGNIIYPDRQLMLFAQDVLSRNPGAK
                     VIFDVKSTRLLAPWIKEHGGKAIMEKTGHSFIKSAMKKTGALVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQNISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQAAIERIQNQFKAVI
                     ESNPNLSWPL"
     gene            complement(13401..14156)
                     /locus_tag="ACFM4Z_000706"
     CDS             complement(13401..14156)
                     /locus_tag="ACFM4Z_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC6020776.1"
                     /translation="MIKIRNIHKTFGENTILHGIDLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(14166..14882)
                     /locus_tag="ACFM4Z_000707"
     CDS             complement(14166..14882)
                     /locus_tag="ACFM4Z_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC6020777.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(14872..15699)
                     /locus_tag="ACFM4Z_000708"
     CDS             complement(14872..15699)
                     /locus_tag="ACFM4Z_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6020778.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            15984..16892
                     /locus_tag="ACFM4Z_000709"
     CDS             15984..16892
                     /locus_tag="ACFM4Z_000709"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HFC6020779.1"
                     /translation="MSTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALTA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
BASE COUNT         4195 a         4889 c         4160 g         3693 t
ORIGIN      
        1 attaaaaaat gccgtctgaa cgtccttacg ttttcagacg gcattgcctt atgcccaaat
       61 aacggtttct tccaagggcc tgcgcgcttt cgtggcgatt tcctgaacgc ggtagccgaa
      121 tgtcgcggca acggacacgc cccattctgc cgcatcgaac aaaccgaact gcccggacaa
      181 tacgcgttcc atctcggcat agttgaaacc ttccaccggg caggaatcga tacctgccat
      241 cgccgcaccc gtcatcatgt tgcccaacgc gatataggtc tggcggcaac accagtcaaa
      301 caaggcgcgc gaatcgtcca aaatcttgat gtcgtcggct tgaaacgcct gatacctcgc
      361 caaagatttt gccatggcat ccggttcggt aacgccgcgc cgtttgaggc tttccaacat
      421 aaacgggctg tcgaagcggg catttttctt tgccaaaaac accaccaaat gactggcggt
      481 atccaaagca tccgccatac cccaagaaaa cggcttgatt gcctgtcgga tttcggggtt
      541 ttgaaccaca acaaactgcc aaggctccga accgaccgaa ctgggcgaca aacgcccgag
      601 ttctaaaata aactgaaaat cctcggcact gattttacgt gccgcatcgt aatgccggca
      661 tgatttgcgg tttttaaatg cggatagaac ctgctctttg cttaatactg ccatcgcaca
      721 tcctttcatt acttgtattg caaattgatt tcaaaccgat acggcactgc cgcaccatac
      781 aatccgtcct cgccatcaag cttcatccgg actttaccca tctctctacg gctgggaaaa
      841 caaagcggcg gcagactcgg gaataacgcc ccaatgcctt tcagacggca tttgggcatc
      901 attgcaaacg cgcctacggg taggcgatat aggcataagc cgaatggttg tggatagact
      961 cgaaattctc gctctcgaca acgaaactct tgatgcgttt gtcggcgatc agcgaagtag
     1021 cgacatcacg caccatatct tccacgaatt tcgggttttc gtaggctttt tcggtaacat
     1081 atttttcatc ggggcgtttg agcaaaccgt agagttggca gcttgcctgc gcctccacat
     1141 aatcaatgac ttcctcgata ccgacttctg catcggcagt caggctgacg gtaacatgcg
     1201 aacgctggtt gtgcgcgcca tattgggaaa tttctttgga acacgggcaa agcgaggtta
     1261 cgggaatcat gaccttcata ctgtagccgt atgccccgtc tttcatttcg cccgtgagac
     1321 agacatcata atccagtaag gaccgaatac cggaaaccgg cgcggttttc ttgcggaaaa
     1381 acggaaaaga aacgctgatt ttgccggcgc gggaatctaa aagcgcgacc atttcggcag
     1441 tcagcctgtg caattgtgca aaatccaagg cttcagaatg ttgctccatc aatgcgacaa
     1501 aacgcgacat atgcgtcccc ttctgctcag caggcaggta aaccgtcatg gtcagacggg
     1561 caacggtgga ctggatgcct tctgcagttt gcagggtaat cggaaagcgc aggtctttga
     1621 taccgacctg attgatcggc agattgcgca aatctcggct ggattgcacg tctgcaatag
     1681 tgttcatgag ttgtttttcc ttaatattca tgtaattgtt gagatgtgtg catggtatgg
     1741 cgtttccgcc ggggaatata ccgtcaatct gcaagtgcga gattatatca ctcgctttta
     1801 gcagctgcat tgattgtttc tatttttcag atagtgataa attttcagat agtgataaaa
     1861 tcttcgatta ttcacatcta cataggcaga gaccaaatga aattcgccac gaaagccatt
     1921 cattccagct acgattgcga cgaacacaac cgcgcgctga tgccgccgat ttatcaaaac
     1981 agtatgttta cgttgcacga gattggcgaa aatgtgcctt accgttattc gcgcctgagc
     2041 aacccgaccc gtcagatttt ggaagacacc gttgccgatt tggaacacgg tgcggcaggc
     2101 tttgcgtttt ccagcggtat ggcggggatt gatgccgtat ggcgtacctt cctgcaaccg
     2161 ggcgatacca tcgtcgcggt cgccgatatt tacggcggcg cgtatgactt attggtcgat
     2221 gtttatcaaa aatggggggt aaatgttgtt tttgccgatt taggcaatcc cgacaatttg
     2281 gacgaattgc tcaaagcgca caaggtcaaa ctggtttggc tggaaacgcc gtccaatccg
     2341 cttttacgct tggtagacat caaagccctt gccgcaaaag ccaaagcgac cggcgcgctg
     2401 gtcggcatcg acaacacctt tgccacgccg tatctgcaac agccgttgga tatgggttgc
     2461 gattttgtgt tccattccgc taccaaatat ttgtgcggcc attccgacgt attgatgggc
     2521 atcgtcgttg ccaaaaccaa agagctggct cagcctttgc acgatatgat ggtgcatacc
     2581 ggtgcgattg caggcccaat ggactgctgg ctggtgttgc gcggcatcaa aacgcttgcc
     2641 ctgcgtatgg aggcacactg caaaaacgca ctcgaaatcg cgcgccgtct ggaagaccat
     2701 cctgccattg aaaaagtgtt ccatcccggc ctgccgtcgc acgaacatta tgaactcgcc
     2761 caagcgcaaa tgcctaaagg catcggcggc gtggtgacgg tttatctcaa aaacgacacg
     2821 cgcgaagcgg caaacagcgt gattaaaaac atgaaactgg tcaaaatggc ttccagcctc
     2881 ggcggcgtgg aaagtttggt caaccattgc tattcccagt cccacagcgg cgtgccgcat
     2941 aatgtgaaaa tggaaatggg catcaaagtc ggtctgctgc gtttctccat cggcatcgaa
     3001 gacgcggacg atatttggaa cgatatttcc gccgcactcg atacaacttt gtaaactgta
     3061 aaaatgccgt ctgaaaccat ggtttcagac ggcatttcaa ttaacccggc cgaaaatcaa
     3121 cgcttcaaca tctttgccgc ctcaatagcg taataggtca aaatcccgtc cgcacccgca
     3181 cgtttgaatg ccagcaggct ttccaaaacc actttgccgc cgtccagcca gccgttggca
     3241 atcgctgcct gcaacatcgc gtattctccc gaaacctgat aggcataagt cggtacgccg
     3301 aactcgtcct tcacgcggcg gacaacgtcc aaatacggta aaccgggctt caccatcacc
     3361 atatccgcac cttcctgaat gtccaacgcc acttcattca gcgcctcatc ggtatttgcc
     3421 ggatccatct ggtaggtttt cttgtccgcc ttgcccaaat tgcccgaact gcccaccgca
     3481 tcgcggaacg gcccgtaaaa cgccgaagca tatttggcgg aatacgccat aatccgcgtg
     3541 tggatatgcc cggcatcctc caacgcctcg cgaatcgcac cgatacgccc gtccatcata
     3601 tcggaagggg caaccacctg cgcgcccgct tcagcgtggc acaaagcctg cttgaccaaa
     3661 acctctacgg tttcatcgtt catcacataa ccgttttcgt ccgtcagccc gtcctgaccg
     3721 tgaaccgtat aaggatcgag cgcgacatcc gtcataatgc ccagttcggg aaacctctcg
     3781 cgcagggcgc ggacggctgc cggcacgagt ccttcgggat tgtacgcttc ctctgcacgc
     3841 tcggttttgt ttgccgtaac cacgggaaag agtgccaaca tcggaatacc gagctttacc
     3901 gcctcttccg ccgtaaacag cagcctgtcc aaactttgac gcttcacacc cggcatagaa
     3961 ggcacatcct cctcgcgcgc cgacccctcc aatacgaaca ccggataaat caaatcatcg
     4021 gcggtcagcg tgtgttcgcg catcaggcgg cgtgaaaaat cgtccctgcg catacggcgc
     4081 atacgcgaag ccggaacatt gcggtaagga aactgcataa gccctccaat catcctgtca
     4141 acaattcaaa ccatacggaa gccgccgcaa cttgcagaca gcatcgggcg gcaatcaaaa
     4201 tccgttttca ccggaaaggg tttcggcccg ccgaaatgcc gcgccgaagg acggcgtccc
     4261 gcccattaaa gaacaatagc aaaacaatag cttcaggctg tcagccttcg atattcttac
     4321 gccacaatac caaaagtttc ccgatatgct gaacaggttg cgcgtcaacc gcctcacata
     4381 aggcagtgca gatttcgata cgttcggcac ggtcgtcgcc gaatacgcgc actttaatca
     4441 gctcatgcgc cgtcaatgcc gcatcggttt ccttgatgac cgcgtccgtc agaccctgct
     4501 gaccgaccat cacaacagga tggagatggt gcgcgcgcgc tttcagttcc aaaatttctt
     4561 tggtgttcaa tttggtatcc gtcattttcc tactgcttga ataaaaagaa taatgcggca
     4621 ttgtacgcga tttggcacga catcggcaaa attaacgtac aatacgcggt ttccattccg
     4681 acccgcgaac cactatggct gtacgttcca aatcctcaaa agcgtggctg cacgaacacg
     4741 tcaacgacca ctacgtccat atggcgcaaa aagacggcta ccgcgcccgt gccgcataca
     4801 aacttttgga aatcaacgac aaagacaaat taatcaaacc cggcacggta cttgccgact
     4861 tgggcagcgc gccgggaagc tggtcgcagg ttgccgccaa gctggccggc ccttccggcg
     4921 cggtattcgc tttggacatc ctacccatgg aagccatagg gggcgtctcc ttcattcagg
     4981 gcgacttccg cgagaacgac gtactggcac aatttgaaac cttgttggac aaccgcccgc
     5041 tcgaccttgt aatttgcgat atggcgccca atatgtcggg aaacgccgta agcgatcagg
     5101 cacgcagcct ctatctgtgc gaattggccc ttgactttgc ctcgcaacac ctgaaaacag
     5161 gcggcagctt tttagtcaaa gtctttcagg gagcaggcta tcaggaatac atggcagcca
     5221 tgcgcgaaat tttcggcacg gtgcagacgc gcaaacccga agcctcacgc aatcgctcca
     5281 gtgagattta tttattgggc aaaaataaac gctgacaata cagacggcgt gctttacaat
     5341 catttccgtt ttacacctca attatggagc cttgctaagt ggggaacacc tttaaatcaa
     5401 tccttgtctg ggtcgccttg ggtatcggcc tgatggctgc gttcaacgct ttagacggta
     5461 aaaaagaaga caacgggcaa atcgaatact ctcagttcat ccaacaggtc aacaacggcg
     5521 aagtatccgg cgtcaacatc gaaggatccg tcgtcagcgg ctacctgatt aagggcgagc
     5581 gcaccgacaa aagcactttc ttcaccaacg cgcctttgga cgacaaccta attaaaacac
     5641 tgctcgacaa aaacgtccgc gtaaaagtaa cgccggaaga aaaaccgagc gcgctggctg
     5701 ccctgtttta cagcctgctg cccgtcctgc tgctgattgg cgcatggttc tacttcatgc
     5761 gtatgcagac gggcggcggc ggaaaaggcg gcgcattctc attcggtaaa agccgcgccc
     5821 gcctgctgga caaagatgcc aacaaagtga cctttgccga tgtcgccggc tgcgacgaag
     5881 ccaaagaaga agtacaggaa atcgtcgatt acctcaaagc gccgaaccgc tatcaaagcc
     5941 tgggcgggcg cgtgccgcgc ggcatcctgc tggcgggcag cccgggtacg ggtaagacgc
     6001 ttttggcgaa agcgattgca ggcgaagccg gcgtgccgtt cttcagcatt tcaggttccg
     6061 actttgtcga aatgttcgtc ggtgtcggtg cgagccgcgt ccgcgatatg ttcgagcagg
     6121 cgaagaaaaa cgccccctgc atcatcttta tcgacgagat tgacgcagtc ggccgccaac
     6181 gcggcgcagg tttgggcggc ggcaatgatg agcgcgagca aacattaaac caattgttgg
     6241 ttgaaatgga cggttttgag agcaatcaga ctgtaattgt gattgcggca accaaccgcc
     6301 ccgacgtact cgatcctgcg ctgcaacgcc ccggccgttt cgaccgccaa gtggttgtcc
     6361 ccctgccgga catccgaggg cgcgaacaga ttttgaacgt ccattctaaa aaagtgcctt
     6421 tggacgaatc tgtggattta ttgtccctcg cgcgcggcac gccgggtttt tccggcgcgg
     6481 atttggcgaa cttggtcaac gaagccgccc tgtttgccgg ccgccgcaat aaagtcaaag
     6541 tcgatcagag cgattttgaa gacgccaaag acaaaatcta tatgggtccg gaacgccgca
     6601 gtatggtgat gcacgaagac gaaaaacgtg cgacggcgta tcacgaatcc ggacacgcga
     6661 ttgttgccga aagcctgccc tttaccgacc ccgtccacaa agtaaccatt atgccgcgcg
     6721 gacgtgcgct gggtctgact tggcagcttc cggagcgcga ccgcatcagt atgtataaag
     6781 atcagatgtt gagccagctc tccatcctgt tcggcggacg gattgccgaa gacatcttcg
     6841 tcggacgcat ctccaccggc gcatcaaacg actttgaacg cgcaacccaa atggcgcgcg
     6901 aaatggtaac gcgctacggc atgagcgaca aaatgggcgt gatggtttat gcggaaaacg
     6961 aaggcgaagt cttcttggga cgcagcgtaa cccgttcgca aaacatttcc gagaaaaccc
     7021 agcaggacat cgacgcggaa atccgccgga ttttggacga gcaatatcag gttgcctaca
     7081 aaatcctcga tgaaaaccgc gacaagatgg aaacgatgtg caaagccctg atggaatggg
     7141 aaaccatcga ccgcgatcag gtactggaaa tcatggcggg caaacaaccc agcccgccca
     7201 aggattacag ccacaacctg cgcgagaatg cggacacggc ggaagataac gcgccgcacg
     7261 ccccggctcg ggaagaaacc gaagcacctg ctccggcaga caccgcttcg acagagtccg
     7321 agcagcagcc tgaaaacaag gcttaacttc ccgaacaaac ggcagcccgc aagctgccgt
     7381 tttcccatcc tgttttcaga cggcattttc cgctcaatgc cgtctgaaac gccaaagccg
     7441 tatcggtttt tgcgaatctg cgatatggcg gtttaaattg aaatcagacg aggcaaggct
     7501 gtaccggttt aaaattaaac cgctataata ctgcccttcg aatacacgcc cgcattggtg
     7561 atgcccgtga aaaaagttga aaaaaacatc ttggtgctgc acggcgcgga caaaatgttc
     7621 gagctggtcg ataaggttga ggattacccg cactttctgc cgtggtacag caaaaccgag
     7681 gtcatcgggc gtagcggcaa cgaactgaag gcacggctgt ttatggacta tatgcacgtt
     7741 cgccaatcat tcgccacgca caaccgcaac attccgggca gggaaatccg tatggaactg
     7801 ctcgagggtc cgttcaaaac cttacgtgga acgtggaaat ttatcgattt gggcgacgat
     7861 atgtgcaaaa tcgaattcaa tttggaatac gatttttcca atgccgtttt gtccgcctta
     7921 atttcccccg tcttcaacca cctttccgcc acgctggtcg aagcgttcgt caaagaggca
     7981 gaccgccgtt atgcttgaaa ttgagattgt gtacgggctg tctgataagc aggttttgaa
     8041 aaatatgcag cttgccgagg gaacaaccgt ccgcgccgcc gcactgcaaa gcggtttgga
     8101 cggcatattt gaagatttaa acctgcattc cgcgcctttg ggcattttcg gcaaagccgt
     8161 caaagacgac acgcccctgc gcgacggcga ccgcatcgaa gtgtaccgcc cgctgttgat
     8221 cgaccccaaa gaagcgcgcc gcaaacgcgt tcaaaatcaa gaagaataac catgccgtct
     8281 gaagccttca gacagcattc gacagaaaca cggaaaatat catgtcaaac aaaatcaaaa
     8341 tgcttgtcgg attgggcaac cccggcaaag aatacgaaca aacccgacac aatgcaggct
     8401 tttggttcct cgacgaactg gcttggaagt ggaaggcttc atttaaagaa gaaaaaaaat
     8461 tcttcggcga agtcgcccgt gccgccctgc ccgacggcga tgtctggctg ctcaagccga
     8521 ccacgttcat gaaccgttcc ggacaggcgg ttgccgccct tgcgcagttc tacaaaatca
     8581 aacccgaaga aatcctcgtc gtccacgacg aactcgacat tccctgcgga cggattaaat
     8641 tcaaactcgg cggcggcaac ggcggacaca acggcttgaa agacattcag gcaaaactcg
     8701 gcacggcaga ctattaccgc ctgcgcctcg gtatcgacca cccgggcgac cgcaacctcg
     8761 tcgtcggcta tgtcctgaac aaacccagta cggaacaccg ccgacagatt gacgatgccg
     8821 tcgccaaatc cctgcaagcc atacccgaca tccttgccgg caaatgggaa gaagcaaccc
     8881 gcttcctgca cagcaaatga cccgatgccg tctgaagccc tttcagacgg catgttcccg
     8941 atttccatat ccgaacagtc atgaccgaac tcaagcagct tatccaaacc gaatccatcc
     9001 ccgtcatcga agaaaccctc gatttcctgc tgtacgaatg cagcatcgac gacgcaccat
     9061 ccgccgaaga agtggcacaa tggcgcgaca tacttgccgc acgcggcggc aaattcctgc
     9121 gcctgtccaa aatctgccaa acgtggctgg acgaggaggc ggcatgaagc tgccgcgcaa
     9181 ccgcttcagc ctgctttccg cattgtggtt tgccggcggc atctattcgc tgctcttcaa
     9241 agctgccgac accgcgccgc cgccgtttcc gcatttcgac aaagcagcac accttgccct
     9301 gtttttcgca caaatctggc ttttgaccaa agcattcaaa accggaaaac ttcccatccc
     9361 ctaccgcagc ctgatggtct ttgccctctg tttcgccctc ttcagcgaat gcgcgcaggc
     9421 atggtttacc gcaacgagaa ccggcagttt gggcgatgtt cttgccgata tggcaggtac
     9481 ggttctcgca ctctttgccg cccgcgccgc cgaccgcccg gactgaatcg gtttatttcc
     9541 cccaaacagg atgcacctct tcccgataca tcctattttt ccccttacat atcaatccca
     9601 tctcataaac aaaaccgaca aatcgtttac aatatattta cactacatca gattacaaat
     9661 atactcgacc cagttgcaga atcggcggtt tcacaaaccg tttcggattg cacgacacaa
     9721 aagaaaaacc gattttattg ccttaaagga gtattcatga acctgcatgc aaaggacaaa
     9781 acccagcatc ccgaaaacgt cgagctgctc agtgcgcaga agccgattac cgactttaag
     9841 ggtctgctga ccaccattat ttccgccgtc gtctgtttcg gcatttacca catcctgcct
     9901 tacagcccgg atgccaataa aggtatcgcg ctgctgattt tcgttgccgc actttggttt
     9961 accgaagccg tccacattac cgtaaccgca ctgatggtgc cgattctcgc cgtcgtactc
    10021 ggtttccccg acatggacat caaaaaggcg atggctgatt tttccaaccc gattatctac
    10081 atttttttcg gcggcttcgc gcttgccacc gccctgcata tgcagcggct ggaccgtaaa
    10141 atcgccgtca gcctgttgcg cctgtcgcgt ggcaatatga aagtggcggt tttgatgttg
    10201 ttcctcgtta ccgcctttct gtccatgtgg atcagcaaca ccgccaccgc cgcgatgatg
    10261 ctgcctctag caatgggtat gctgagccac ctcgaccagg aaaaagaaca caaaacctac
    10321 gtcttcctcc tgctcggcat cgcctattgc gccagcatcg gcggcttggg cacgctcgtc
    10381 ggctcgccgc ccaacctgat tgccgccaaa gccctaaatc tggacttcgt cggctggatg
    10441 aagctcggcc tgccgatgat gctgttgatt ctgcccttga tgctgctctc cctgtacgtc
    10501 atcctcaaac ctaatttgaa cgaacgcgtg gaaatcaaag ccgaatccat cccttggacg
    10561 ctgcaccgcg tgatcgcgct gttgattttc cttgccacag ccgccgcgtg gatattcagc
    10621 tccaaaatca aaaccgcctt cggcatttcc aatcccgaca ccgttatcgc cctgagtgcc
    10681 gccgtcgccg tcgtcgtctt cggcgtggcg caatggaagg aagtcgcccg caataccgac
    10741 tggggcgtgt tgatgctctt cggcggcggc atcagcctga gcacgctgtt gaaaacatcc
    10801 ggcgcgtccg aagccttggg acagcaggtt gccgccacct tttccggcgc gcccgcattt
    10861 ttggtgatac tcatcgtcgc cgccttcatt atttttctga ccgagttcac cagcaacacc
    10921 gcctccgccg cattgcttgt accgattttc tccggcatcg ctatgcagat ggggctgccc
    10981 gaacaagtct tggtattcgt catcggcatc ggcgcatctt gtgccttcat gctgccggtt
    11041 gccacaccgc ctaacgcgat tgtgttcggc acgggcttaa tcaagcaacg cgaaatgatg
    11101 aatgtcggca tactgctgaa catcctctgc gtagtattgg ttgctctgtg ggcttatgct
    11161 gtactgatgt aaaccatcga cctaaacaac aagaccgtct gaaagaatat ttttcagacg
    11221 accttgaagt ttgtcgttca gacacaattt gtcgaatcat tcaaaaccgg attctaacga
    11281 aaggaaaccc atgattatcc tgcacaccaa caaaggcgac atcaaaatcg aactcgattt
    11341 cgacaaagcc cccgttaccg ccaaaaactt cgagcaatac gtcaaagacg gcttctacga
    11401 cggcgtaatc ttccaccgcg tcatcaaagg cttcatgatt caaggcggcg gcatggatga
    11461 aaacatgaac gaaaaagaaa cccgcgatcc gattcaaaac gaagcgtcca acggcctgcc
    11521 caacgataaa tacaccatcg ccatggcacg cacttccgac ccccattccg ccagcgcgca
    11581 attcttcatc aacactgccg acaacgcttt cctgaacttc cgttctaaag agctgtacgg
    11641 caaaaccgtc gtccaagact ggggctatgc agtattcggt aaagtcgttg acggttttga
    11701 cgttgtcgat gccatcgaag gcgtatccac caaacgccac ggctaccatg acgacgtacc
    11761 gagcgaacct gtcgtcatca ttaaagccga agcggtataa accggcaatc cgaaagcagc
    11821 ctgcacaaag gctgcttttt ttgtttcagg cggcataaaa tccaagcaca tatgccgtct
    11881 gaacacctcc cgcaagcctt cggacaatgt tgcaacgtgt taaaatagcc tcctttcctg
    11941 aaaccacaac aaggaaacca tcatggcaaa tattgcccgc gacatcttca aagcctacga
    12001 catccgaggc attgtcggca aaaccctgac tgacgatgcc gcctatctca tcggcagggc
    12061 catcgccgcc aaagccgccg aaaaaggcat gacccgcatc gcgctcggac gcgacggacg
    12121 cttgagcggt cccgaactga tggagcacat ccaacgcggc tttaccgaca gcggcatcga
    12181 cgtcctcaac gtcggtatgg tcgccacgcc catgctctat tttgccgcta tcaacgaatg
    12241 cggcggcagc ggcgtgatga ttaccggcag ccacaatccg cccgattaca atggtttcaa
    12301 aatgatgctc ggcggcgaca cgctcgcagg cgaagccatt caagaacttt tagccattat
    12361 tgaaaaagac ggttttgttg ccgccgacaa acaaggcaac gtaaccgaaa aagacatctc
    12421 cggcgaatac cacaaccaca tcgtcggcca catcaaactc aaacgcccca tgaaaatcgc
    12481 catcgacgca ggcaacggcg taggcggcgc attcgcaggc aaactctaca aaggcttggg
    12541 caatgaagtt accgaacttt tctgcgacgt ggacggcaat ttccccaacc accatcccga
    12601 cccatccaaa cccaaaaacc tgcaagattt gattgccgcg ctgaaaaacg gcgatgccgg
    12661 aatcggcctg gcgtttgacg gcgatgccga ccgcttgggc ctggttacca aagacggcaa
    12721 cattatttat cccgaccgcc aactgatgct gttcgcgcaa gacgtgttaa gccgcaatcc
    12781 cggcgcgaaa gtcatcttcg acgtgaaatc cacccgtctg cttgctcctt ggattaaaga
    12841 acacggcggc aaagccataa tggaaaaaac cggccacagc tttatcaaat ccgccatgaa
    12901 aaaaaccggc gcgctggttg ccggcgaaat gagcggacac atcttcttca aagaacgctg
    12961 gttcggcttc gacgacggtc tgtacgccgg cgcacgcctc ttggaaatcc tgtccgcctc
    13021 cgataatccg tccgaagtgt tgaacaacct gccgcaaaac atttccacgc ccgaactcaa
    13081 catcgccctg cccgaaggca gcaacggcca tcaggttatc gacgaactcg ccgccaaagc
    13141 cgaattcgaa ggcgcaaccg aaatcatcac catcgacggt ctgcgcgttg aatttcccga
    13201 cggcttcggt ctgatgcgtg cttccaatac cacgccgatt ctggtgttgc gttttgaggc
    13261 ggatacgcaa gcagccatcg agcggattca aaaccaattc aaagccgtca tcgaaagcaa
    13321 tccaaattta agctggccgc tgtaaacatc gaaaaaatgc cgtctgaagg cttcagacgg
    13381 catttttcac aaatagttaa tcatatcttg gtagattgga tttggcttaa aaatctccgc
    13441 gtccgttcgt gtttggggtg atcgaacaac tctttcgggc tgccttgttc gacaataacg
    13501 ccgccgtcca tcacgacaac ggtagtcgcc acttccagcg caaacttgat ttcgtgggta
    13561 acgacgacca tcgtccaacc ttcccgcgcc aattccttca tggcgttcaa cacgtcttgc
    13621 accaactcgg ggtcaagcgc ggaagtgggt tcgtcaaaca acatcagctc gggctgaatc
    13681 gccaatgctc gggcaatgcc gacgcgctgc tgctgaccgc cggaaagctg gtagggatac
    13741 aaatccactt tgtcgcccaa gccgactttt tccagcagtt tcagagcctc ttcgcgcgct
    13801 tgggcggcag gcttgccctg tacggcaacc ggtccttcca tcacgttttc caaggcggtt
    13861 ttgtgcggaa agaggttgta ttgttgaaac accatgcctg atttgcggcg cagtgccaaa
    13921 atatcgtgtt tgcttggttt tttagaaaaa tcgattttca gcggtcgctc gttgtcgaac
    13981 tcgatttgtc cgtcttcggg catttccaac gcgtttaggc agcgcagaaa cgtcgttttg
    14041 cctgagccgg aaggcccgag gatgacgacc acctgccctt tgcacacatc caaatcgatg
    14101 ccgtgcaaaa tggtattttc gccgaaggtc ttatggatat tgcggatttt aatcatgaca
    14161 actccttatt tggcgacgta gcggtcgaaa cgtttttcca aacgcgcctg aatcaggaac
    14221 agcactttac aaaaacacca gtaaaccaaa gcggcttcga tatagacggg caaaaagtca
    14281 taagtgcggt ttgccgtttc ctgcgcgacg cggaataatt ccgttaccgt cacgactgcc
    14341 gcgagcgagg tgtttttaaa caaaccgata aactcgttgc tcaaaggcgg cacggcaacg
    14401 cggaatgcct gcggcgcgac aatgcggcgg aacgtctgca tataggtcat gccgatggag
    14461 aaacctgctt cccattggcc tttcggtacg gacaaaattg ccgcgcgtat ggtttcggaa
    14521 gcatatgcgc cgacattgag cgaaaagccg atgatggcgg caggaatcgg gtcaatatag
    14581 atgccgacgg aaggcagccc gtaaaacaca atcacaagct gaaccaacag cggcgtaccg
    14641 cgaatgacgg aaatataaaa ttccaccaat ttcagcagga ttttccgcac gatgccgccg
    14701 gcgggcataa tccgcaccaa agccacggct accgcaatca tcataccgat aacgaaagaa
    14761 gctgccgcca aaggcagaga gaccgcgaag ccggctttga ccataggcaa aaacgcgctg
    14821 acaatcatat cggcgcgtgt ttccgtcata aacggcagcg aagcaaggaa attattgaac
    14881 actgatgtct tttccgaaga attgttcgcc cagttttttc agcgtaccgt cggctttcag
    14941 ctcgttgatt gccgtactga atttcgccac ggcttcgtca ttgcccttgt tgacaatcag
    15001 gccggaaccg actttttcat cggcaggtgc ggaccaaacg attttcacgc ccgcattcgg
    15061 gtttttcttc agatagtcca aaaccgccaa ttcgtcgttc agggttgcat cggcacgttt
    15121 ttgttcaatc agggtcagcg attgcgccaa accgtcaaca gccaccaaat ctgcgcctgc
    15181 agctttggct ttttcgccgt agttgctggt cagggattgt gcggttttca cgcctttgat
    15241 gtcgtcgata gatttgatgt tgctgtcgtt acgggcaacc aatacggcac cgctccagct
    15301 gtaaggatcg gatttgtcga atgtcgcttg gcgttcgggg ctggtcagac cgacttggtt
    15361 tgccaccacg tcgaaacgcc ccgccttcaa acccgccatc atcgaatccc attgcgtttc
    15421 tttaaactcg actttcacgc ccagtttttc cgccacggcg cgggttactt ccacatcgta
    15481 accggtcagt ttgccgtcct tgtcgtggta ggtaaacggt gcgtaagtgc cttccgtgcc
    15541 gacggtaacc gtgcctttat tgttgatgcg ctcgattaaa gaaccggaaa ctgccgattg
    15601 tgcaggcgcg gaagatgctc cgctgccgcc ttccgaaccg ccgcaggccg ccaaaaccaa
    15661 tgcggcaatg ccgccgagta cgaatttttt caacatcatc ctctcctgta aaaagtgtgc
    15721 aagtgcggca ttctaagtga aattgcggca aacacaaaga atcctttttt tatatggtta
    15781 ttccaaatcg gaatggaaaa gatatgccga agccgccgga agcggaaata aagggggcgg
    15841 gaaaggcggc gggcggcacg aaaccgaaaa gcggcatcag gtatgcaaac ggcggattca
    15901 cgcacgccgt tccggcaaaa atccgcctgc cgattcggtg ctataatcca tccgaaacag
    15961 atgacaccaa aaaggaacat ctcatgagca cgccccaatc cgccatcatt cccgaccacg
    16021 cccaagccgg catctttatc gaagccgact tcgccgccaa ccgcctcaac gatattaaag
    16081 ccgcctgccg cgcttcgctc gacgcgttaa ctgccttgaa agcccgcttt ccggacgata
    16141 ttttgggtct gaccatcgcc ttcggcagca aagcctgggc aacattcgga catacggacg
    16201 aaggcagtga aatcaaaccc ttctccgaaa tgggcaacgg gcttgcgccg tccacgcagc
    16261 acgatatgtc catccacatc caatccttcc gccaaaacgc cgcctacgcg cttgcccaat
    16321 ccgttttggg cgcgttcggc gacagcatat gcgtcgcgtc cgaagaacac ggtttgcgcc
    16381 tgtatcagga tcgcgggctg gacggtttcg tcgacggcac ggaaaacccg cagggcgatg
    16441 aaaatgtccg cgaagtcgcc atcatccccg aaggaaaacc cgatgcgggc ggcagctatg
    16501 tcctgctgca aaaatacctg cacgatttga aaaaatggga tgccgtcccc gtcgccgaac
    16561 aggaggcctc ggtcggacgc ggcaaggaaa ccgacgacga attcggccgc gatgtccgcc
    16621 tgcccgattc gcacctcggc cgcgtcaacc tgaaagaaaa cggagtcggc ctgaaaatcg
    16681 tccgccgcag cctgcccttc ggcaaaatca gcggcgaaca cggtttgatg tttaccgcat
    16741 actgccgcac gctgcacaat atcgaagcgc aattattgag tatgttcggc gatacggacg
    16801 gcaaaaccga cctgctgctc cgacatctct ccgccgccgt ttcgggcgga tactactacg
    16861 cgccgtctgt cgaacgcctg caaaacctct aagccggacg atgccgtctg aaaccatttt
    16921 tcagacggca ttttccc
//
LOCUS       DAXHNE010000027        16896 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.027, whole genome shotgun sequence.
ACCESSION   DAXHNE010000027 DAXHNE010000000
VERSION     DAXHNE010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16896
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.027"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(65..1312)
                     /gene="fabF"
                     /locus_tag="ACFM4Z_000710"
     CDS             complement(65..1312)
                     /gene="fabF"
                     /locus_tag="ACFM4Z_000710"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFC6020780.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            complement(1468..1704)
                     /gene="acpP"
                     /locus_tag="ACFM4Z_000711"
     CDS             complement(1468..1704)
                     /gene="acpP"
                     /locus_tag="ACFM4Z_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFC6020781.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            1936..2943
                     /locus_tag="ACFM4Z_000712"
     CDS             1936..2943
                     /locus_tag="ACFM4Z_000712"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFC6020782.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTGNRTKPV
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPKPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDAMIQNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVHGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGRPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGGDSADKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            2959..3138
                     /locus_tag="ACFM4Z_000713"
     CDS             2959..3138
                     /locus_tag="ACFM4Z_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020783.1"
                     /translation="MPSERTFCRSDGIFISFFRLTPLENPLRAALFETKQTPAANRTI
                     LIRSQSKSGGKLLNI"
     gene            3276..3599
                     /locus_tag="ACFM4Z_000714"
     CDS             3276..3599
                     /locus_tag="ACFM4Z_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020784.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            3686..3967
                     /locus_tag="ACFM4Z_000715"
     CDS             3686..3967
                     /locus_tag="ACFM4Z_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020785.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            4020..4493
                     /locus_tag="ACFM4Z_000716"
     CDS             4020..4493
                     /locus_tag="ACFM4Z_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HFC6020786.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            4507..4833
                     /locus_tag="ACFM4Z_000717"
     CDS             4507..4833
                     /locus_tag="ACFM4Z_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020787.1"
                     /translation="MPSEALSGLQTAFCFPTVYKLSLFLDRNNDLIYLKRLEDMIMGF
                     WNGVAKAVGEGMIEAANEHKALKMEYAEKSSEELHEIVKSDGFFKNSTREKGAAYAVL
                     KERGEV"
     gene            5031..7721
                     /gene="glnE"
                     /locus_tag="ACFM4Z_000718"
     CDS             5031..7721
                     /gene="glnE"
                     /locus_tag="ACFM4Z_000718"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFC6020788.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRDTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNGIKSLVRPFVFRKYLDYGAYEAMRKLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTRETLKKLAELGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPASPEQRQRLAESMGFDDYADFLNAL
                     NQHRNQVNRLFNEILSEPGEQTQSNGEWQWVWQEKPDEEGRQCRLKEHGFDAEAVAAR
                     LDQIRHGHKYRHLSAHAQPRFDALVPLSVRAAAAQNNPTDTLMRLFDFLENISRRSAY
                     LAFLNEHPQTLAQLAEIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWDKLAAELS
                     DGIKACGGDTEAQMDTLRHFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     SCAWADMPKKHRDTPQFAVIGYGKLGGKELGYASDLDLVYLYDDPHPDAGDIYGRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGSPEIQTAFDRIRTEILTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARKYPQLLDNYGNIALLNIAADCGLIDKTLAGQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWREVFGEEAATA"
     gene            complement(8021..8713)
                     /locus_tag="ACFM4Z_000719"
     CDS             complement(8021..8713)
                     /locus_tag="ACFM4Z_000719"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFC6020789.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(8821..10014)
                     /locus_tag="ACFM4Z_000720"
     CDS             complement(8821..10014)
                     /locus_tag="ACFM4Z_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC6020790.1"
                     /translation="MSNQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(10143..10880)
                     /gene="pxpA"
                     /locus_tag="ACFM4Z_000721"
     CDS             complement(10143..10880)
                     /gene="pxpA"
                     /locus_tag="ACFM4Z_000721"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFC6020791.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGRVKAVNGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(10867..11796)
                     /locus_tag="ACFM4Z_000722"
     CDS             complement(10867..11796)
                     /locus_tag="ACFM4Z_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HFC6020792.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDCLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQVYLNQIRRITHEAG"
     gene            complement(11793..12440)
                     /gene="pxpB"
                     /locus_tag="ACFM4Z_000723"
     CDS             complement(11793..12440)
                     /gene="pxpB"
                     /locus_tag="ACFM4Z_000723"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFC6020793.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            12624..14831
                     /gene="uvrD"
                     /locus_tag="ACFM4Z_000724"
     CDS             12624..14831
                     /gene="uvrD"
                     /locus_tag="ACFM4Z_000724"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFC6020794.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPVDVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     NAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTIENLQTASNEQGITLWQAACNAGAK
                     ATKVAAFVRLIEALRNQVGQMPLPEIIVGILKDSGLTEHYKTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFEILPENISDDPAFPILAFLSNAALESGENQAGADEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKTAYDSYGSPRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
     gene            15188..15727
                     /locus_tag="ACFM4Z_000725"
     CDS             15188..15727
                     /locus_tag="ACFM4Z_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6020795.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGETGQK
                     LWMEEWEYPPIGVYFANDPSGGHAMFALDYRECGKDGEPKVVFVEQESDFEIVELAPD
                     FETFIRSLRHEDEFIDEEI"
     gene            complement(15917..16132)
                     /locus_tag="ACFM4Z_000726"
     CDS             complement(15917..16132)
                     /locus_tag="ACFM4Z_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020796.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNSKLVQELEQQLIEAYDLMDTKG"
     gene            complement(16256..16615)
                     /locus_tag="ACFM4Z_000727"
     CDS             complement(16256..16615)
                     /locus_tag="ACFM4Z_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC6020797.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            16668..>16896
                     /locus_tag="ACFM4Z_000728"
     CDS             16668..>16896
                     /locus_tag="ACFM4Z_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6020798.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTATEPDSIVYTDCYRSYDV
                     LDVSEFSHFRINHSTHFAERQNHINGIENFWN"
BASE COUNT         4190 a         4486 c         4351 g         3869 t
ORIGIN      
        1 ctataaaaat gccgtctgaa acggtttcag acggcatttc gatgtcggcg gcggctttgc
       61 ggaatcagcc tttgaagcgt ttgaagacca gcgtgccgtt ggtgccgccg aagccgaagg
      121 agttggaaat ggcaacgtcg atttccgcgt cgcgcgcttc gttggcgcag tagtccaaat
      181 cgcagccggc ttcaacgtct tgttcaaaaa tgttgatggt cggcgggatt ttgccgtcgt
      241 gtatcgccaa aatgctgtac acggcctcca cgccgcccgc cgcgccgagc aggtggccgg
      301 tcatggattt ggtcgagctg acgacggttt tgtaggcgtg ttcgccgaat gcgcgtttga
      361 gggctttggt ttcgttggca tcgcccaagg gggtggacgt gccgtgcgcg ttgacgtaat
      421 ccacgtcttc gggattgatg ccggcatctt tcagcgcgcg ggtaacggca agggcggggc
      481 cttcttcgtt cggcgcggtg atatggtaag catcggaact catgccgaag ccgacgattt
      541 cggcgtagat tttcgcgccg cgttttttgg cgtgttccaa ttcttccaac accaatatgc
      601 ccgcgccttc gccgataacg aagccgtcgc ggcctttgtc ccacggacgg gaagcggtgg
      661 cggggtcgtc gttgcgggtg gagagggctt tcatcgcggc aaaaccgccc acgcccaaag
      721 tgctgattgc gccttccgcg ccgccggcaa ccattatgtc cgcgtcgccg tatttgatca
      781 gtcgggcgga attgccgatg gcgtgcgcgc cggtggtaca ggcggaaacc atcccgtagc
      841 tcgggccgcg gtagcctttg aggatggtaa cgtgtccgga aatcaggttg atcagcgaac
      901 cggggataaa gaaagggttg attttgcgcg cgccgccttc gattacggct ttgccggtga
      961 cctcgatgct gggcagtccg ccgatgccgg aaccgatgtt cacgccgatg cggtctttgt
     1021 cgaggttttc cacatcgtcc aagcccgaat cggcgattgc ctgcaatgcg gcggcaatgc
     1081 cgtagtggat gaatacgtcc atccggcgcg cttctttcgc gctgatgtat tgtccgatgt
     1141 cgaaaccgcg cacttcgccg gcgacacggc tgttgatgtc ggatgcgtca aagcgggtaa
     1201 tcgcgccgat gccgcttttg ccggcgagca gggtgtccca agcctctgcg acagtgttgc
     1261 cgacagggga aacctgacct aagcctgtaa tgactactct tctctgactc atgataacct
     1321 cgctgttggt tgtcggaatg ggggcatatg cggctgtcgt gcagatgccg tctgtaattt
     1381 gcggcagggg ttcaaacagt ttgccatata agggaaaagc ctctattgcg cggtgcagca
     1441 gaggctgttg tgtcgggcga cgaccggtta gccgttgtgg gcattgatgt agtcgatagc
     1501 cagttggacg gtggtgattt tttcggcatc ttcgtcgggg atttcgcagc cgaatgcttc
     1561 ttccaaagcc ataaccagct ccacggtgtc caaagaatcc gcgcccaagt cgtcttggaa
     1621 agaagattcg tttttcacgt cggcttcgtt tacgcccagt tgttcagcaa caattttttt
     1681 aacttgttgt tcgatgtttg acatatcagt cgttccttta tgccttgcgg caggttgttt
     1741 aagggaaata aatcggtggt attgtaccga cttttaatag agttttctat ctaatgacta
     1801 ttatatcaat atttgccgat ttgtacattt ttgggtgcgg cgggttttgt cgttcaagtt
     1861 tgacctgtgt gccgtatgtt tggcgggatt tcggttaaaa tggcggcatt tccatctgaa
     1921 gcagaaagcc ctgtcatgta tccacttgcc cgccgcatcc tgtttgcact cgatgccgaa
     1981 aaagcccacc acttcacgct cgacgcgctc tacacggttt ataaattggg tctgattccc
     2041 gtaaccggca accgtaccaa acctgtaaaa ttgatgggta tggatttgcc caaccctgtc
     2101 ggacttgccg ctggactcga caaaaacggc gaatacatcg acgcattggg cgcgctcggc
     2161 tttggtttca tcgaaatcgg cacggtaacg cccaaaccgc agcccggcaa cccgcagccg
     2221 cgcctcttcc gcgttcccga acaccaaggc atcatcaacc gcatgggttt caacaaccac
     2281 ggtatcgatg ccatgattca aaacatcgaa aaaagtaaat tcagtggcgt attgggcatc
     2341 aacatcggta aaaacgcggt tacacccatc gaaaacgctg ccgatgatta tttaatctgc
     2401 cttgaaaaag cctacgcgca cgcaagctac attaccgtca acatttcctc gcccaacacc
     2461 aaaaacctcc gcgcgctgca aggtggcgac gagttgagcg cattgcttga ggctttgaaa
     2521 aacaaacagg cacagcttgc ctctgtacac gggaaatacg ttccgctcgc cgtcaaaatc
     2581 gcccccgatt tggatgaagc acaaatcgaa gacatcgccc acgttgtcaa atccgtcgaa
     2641 atggacggca tcatcgctac caataccacc atcgacaaat caagtctcgg cagccatccg
     2701 ctcgcaggcg agcagggcgg tttgagcggg cggcccgttc atgaaaaaag taatcgggtg
     2761 ttgaagctgt tggcagacca catagacggc aagctgccga ttatcggcgt aggcggcatt
     2821 atggaaggcg gggactcggc agataaaatc cgcttgggcg caactgccgt ccaggtgtac
     2881 agcggattga tatacaaagg tccggcattg gtcaaagaat gtttgaaggc tttggcgcga
     2941 tgacgcgatc cgcccaaaat gccgtctgaa cgcacgtttt gccgttcaga cggcattttc
     3001 atttcctttt tccgcctgac gccccttgaa aatcccttac gcgccgccct gtttgaaaca
     3061 aagcaaacgc ccgccgcgaa ccggacaatc ttgatacgca gccaaagcaa gtccggcggt
     3121 aagttgttaa atatataagc aattgataca aaaaataatt ttatactgtg gcggacggct
     3181 tgggaaaacg caaaatatga acggcgttga aattcatgat gtggaaaata aggatacggc
     3241 ggataatcaa aagattttat atttaaggat ttgatatgtt taaggacgaa ctaaatgaat
     3301 ttatccgatt gatatccgat ccggaaagtg aattggatga atggtatttg agtgatttta
     3361 aagatgaaca tatatgggaa atgcaaagtt acgaagcatt ttcatgtttg cgggaagcag
     3421 tcccctattt gtttgcttat ccccgatacg ggtatgaact gttggagata atttccgcgt
     3481 taaaagaaac atctgatacg acagaattat tttatgagcc gggtatcgtg cctttgctga
     3541 ttgatttata taaagaagat agttatctgg taaatatggt caaacggata ttcaaatgat
     3601 gaaaggggcg cgggcgtttg cttggcggaa ggcaggtaac tcaatttgaa ggacgcggaa
     3661 gccgatacgt taaaaaggat gaaaaatgga aaacgggcaa cggaataacc ggtttccttt
     3721 ggaaaaacga attttttatc tggagcattc ggggcggtat ctgatgattt gcgccttatc
     3781 ggattattcc caaaacaaac atactgtcgt tatggcaaat ttcatctatc cggatgaaaa
     3841 aacggattgg cggaatttgg atgatttatt caatgagttg gttttagagg aattgcaggc
     3901 ttcatttatg gattggcatc cgactgttga agaggcaatc agccgtcatt tggaagactt
     3961 ttcgtaacag gcgtcaatcg gaaggggcgg aatgacgggg caggcaatcg gagtaaaaaa
     4021 tgaaccttga tttaaccgcg caaaaagtcc gtctttcttg gaaggatatt ctgtgggggt
     4081 atgggaataa atacttgggt tgggctgatg tggcagctta tgcccgaaaa atgacgcttt
     4141 cagatcatga tgaacgtgtg ttcaaactat ctttaatcaa caaatccaat attcttgaat
     4201 taaagcctgt tctggaagat ttggcttcgg aaatgaggga ttattcccct aaaaattggc
     4261 tgtacgtcct cttaagcgat gtattccata gaaaagaaga atttgaggat cctttggggg
     4321 aagttgaaaa aatttatgca gattttgatt atccggaaga aatagaatca tttgtcaggt
     4381 atatgccgcc caaagacggt tatattcctt ctgcccacac ctatgaagaa aatattgccc
     4441 ggttatattc tcactgggaa cactatttga acaacggcgg agggcagggt taaaaccggc
     4501 aatccgatgc cgtctgaagc attatccggc cttcagacgg cattttgttt tccgacagtt
     4561 tataaactgt cgttgtttct tgacagaaac aacgacctta tttatttgaa acgattggag
     4621 gacatgatta tgggtttttg gaatggtgtg gcaaaagcag taggcgaggg aatgattgaa
     4681 gcagccaatg agcataaggc gttgaaaatg gaatatgcgg agaaatcaag tgaggagctg
     4741 catgaaatcg taaagagtga tggttttttt aaaaattcca cacgggagaa aggtgcggct
     4801 tatgctgttt taaaagagcg tggcgaggtg tgaacaggaa acggcggcat ttgccgctgt
     4861 tttttatcgg caggcatccg tctggatgtc ggggcaaggt ttcagacggc atcgaaggtt
     4921 gctatgatat agtggcttga ctttaaaccg gtacggcatc ccctcgccct gtcctgattt
     4981 gaagtgaatc cgccatcatc ttcctgccat tttttcaaac ggaatccgaa atgtccgaca
     5041 accgcctcga caccgcccgc cgccattccc tcttcctcgc ccgccagctc gacaacggca
     5101 aactcaagcc cgaaattttc ctgcctatgc tggacaaggt tttgaccgaa gcggatttcc
     5161 aagcctttgc cgactgggac aaaatccgtg cggaagaaaa cgaggaagaa ttggcgcggc
     5221 agttgcgcgg gttgcgccgt tatgtggtgt cgcagattat cgtgcgcgat ataaaccgca
     5281 tcagcgattt gaacgaagta acccgcacga ttacgctgtt tgccgatttt gccgtcaata
     5341 ccgcgctgga tttcgcctac gcctattatc gggatatgta cggtacgccg atcgggcgtg
     5401 ataccaaatc gccgcagcat ttgagcgtgg tggcgatggg caaggcgggc ggctatgagt
     5461 tgaacgtgtc ttccgacatt gatttgattt tcgtctatcc cgaatcgggc gataccgacg
     5521 gcaggcgcga acggggcaat caggagtttt tcaccaaagt cgggcagaaa ctgattgcat
     5581 tgttgaacga catcaccgcc gacgggcagg tgttccgcgt cgatatgcgg ctgcggccgg
     5641 acggcgattc gggcgcgctg gtgttgagcg aaaccgcgct ggagcaatac ctgattacgc
     5701 aggggcgcga atgggaacgc tacgcgtggt gcaaaggccg cgtggtcacg ccgtatccga
     5761 acggaatcaa atcgctggtg cgccccttcg tgttccgcaa atatctggat tacggcgcgt
     5821 atgaggcgat gcgtaagctg caccgccaaa tccgcagcga agtcagcaaa aaagggatgg
     5881 cggacaacat caaactcggc gcgggcggca tccgcgaagt cgaatttatc gcccagattt
     5941 tccagatgat acgcggcggg caaatgcgcg cgctgcaact gaaaggcacg cgggaaacgc
     6001 tgaagaagct tgccgagctg ggcatcatgc cgtctgaaaa cgtcgaaacc ctgctcgccg
     6061 cctaccgctt tttgcgcgat gtcgaacatc gtttgcaata ttgggacgac cggcaaaccc
     6121 aaaccctgcc ggcttcgccc gaacagcggc aacggcttgc cgaaagtatg ggatttgatg
     6181 attacgccga ttttttgaac gctttaaatc aacatcgaaa ccaagtcaac cggttgttta
     6241 acgaaatttt gagcgaaccc ggagaacaaa cgcaaagcaa cggcgaatgg cagtgggttt
     6301 ggcaggaaaa acccgacgaa gaagggcggc aatgccgtct gaaagaacac gggttcgatg
     6361 ccgaagccgt cgccgcaagg ctcgaccaaa tccgccacgg ccataaatac cgccatcttt
     6421 ccgcacacgc ccagccgcgt ttcgatgcgc ttgtgccgtt gtcggtacgg gcggcggcag
     6481 cgcaaaacaa cccgaccgat acgctgatgc ggctgtttga ctttctcgaa aacatcagcc
     6541 gccgctccgc ctatctcgcc ttcctcaacg aacatccgca aaccctggcg caactggcag
     6601 agattatggg ccaaagttct tgggtggcgg cgtatctgaa caaatatccg attttgttgg
     6661 acgaactcat cagcgcgcaa ttattagata cggcgtttga ctgggacaaa ctcgccgccg
     6721 aactttcaga cggcatcaaa gcctgcggcg gcgacaccga agcgcaaatg gacaccctgc
     6781 gccatttcca gcacgcccaa gtcttccgcc tcgccgtcca agacctcgcc ggactgtgga
     6841 cggtagaatc cctctccgac cagctctccg ccctcgccga caccatcctc gccgccgccc
     6901 tttcgtgcgc gtgggcggat atgcccaaaa aacaccgcga caccccgcaa ttcgccgtca
     6961 tcggctacgg caaactgggc ggtaaagaac tcggctacgc ttccgatttg gatttggttt
     7021 atctctacga cgacccccat cccgacgcgg gagatattta cggacggctc gcccgccgcc
     7081 tgaccaattg gctttccgcc gccaccggcg cgggcagcct ctacgaaacc gacctgcgcc
     7141 tgcgtcccaa cggcgacgcc ggcttcctcg cccacagcat tgccgccttt gaaaaatacc
     7201 agcgcgaaaa cgcgtggacg tgggaacacc agtccctcac ccgcgcccgc tttatctgcg
     7261 gctcgcccga aattcagacg gcattcgacc gcatccgcac cgaaatcctc accgccgaac
     7321 gcgaccaaac cgccttggca ggcgaaatca tcgaaatgcg cgaaaaaatg ttccccaccc
     7381 acccgcctgc cgacagcaac gtcaaatacg cgcgcggcgg cgtggtcgat gtcgaattta
     7441 tcgtccaata tctgatactt gcccacgccc gaaaatatcc gcaactcttg gacaactacg
     7501 gcaacatcgc cctcttgaac atcgccgccg actgcggttt gattgataaa acgcttgccg
     7561 gacaaagccg caccgcctac cgcttctacc gccggcagca gcacaacacc aaactgcgcg
     7621 acgcggcaaa aaccgaagta accggcgaac tgttggcaca ttacggcaat gtcaggaaat
     7681 tgtggcggga agtgttcggc gaagaagcgg caaccgcctg aacaaaaaat gccgtctgaa
     7741 gcctgacaat ctgggtttca gacggtattt tcgtaccgtg ccgttttaag gttgcggcag
     7801 agctaaagcg atttatcggg aatggctgaa acccaaaaac cggattcctc tttcagttac
     7861 agcagcttga tccctttccc ttatccaacg ggggaaggct aggatagggt ggcttgcaaa
     7921 tatacagaac aagggacaag agccaccctc tctccaaccc tctccctccg tacgggaggg
     7981 ggtggattct cgcgggcgaa gcccacgcta cggttagcct tcaccccagc acaaacaatt
     8041 cccgcccgtg cgccttcagc caacttttag cattgtcggt atgcggcgtc agcgtgttca
     8101 ccaaatgcca aaagcgcgga ctgtggtcgg ggtggcggag gtggcagagt tcgtggatgc
     8161 agacatagtc ggcgacgtat tcgggcgtgc cgatcagccg ccagttgagg cggatgccgg
     8221 tgtgcgggcg gcatacgccc caaaaggttt tggcgttgct caggtctgtg gcggtgggcg
     8281 tcagtcctgt ttcggctgcg tgtttttcaa ggcggggcag caggtattcg cgggcgcgtt
     8341 cgttcaacag gcggcgcagg tggtcgattt gtgcggcggt ttcttttcgg ggaagcagga
     8401 tttcagacga cgtgatacgg atatggcttt ggctgtgggt atccagcttg gtctttattc
     8461 cccgatacca aatccactcg ggtaagtttg ggtgggaaac aggatgcacg ggcgttttgg
     8521 caagcgtgtt ccgcaaaatc gtttcgtttg ccgccagcca gtttgctaac gcgtggtctt
     8581 gaaaaaaggg tgggacgttg atgctgaccg tctgcatatt gacggggcgc agaatcagat
     8641 ttttcttggc actgcgtttg agttcgattt cgatgcacaa accgtcggaa agagtatagg
     8701 tgaagcgttt catagttgtg aatgggtttc agaccggata catcgtctga aacaggaatt
     8761 ttccatatca ggcggcaaac ttcggataat atacaaaatc aaacatctgc gctacaaggt
     8821 tcagccgaac aagccgccga tatatttgct gatggtgatg gcgctgagta ctgccatcaa
     8881 accgaccaca atcacgccgg aaacggtgag ccacagcggg tgtttgtagt cgccgacaat
     8941 tttggttttg taggcggcaa tcagaatcag accgagggaa atcggtaaaa tcaggccgtt
     9001 taatgcgcct acgaacacca gcacctgcgc cggtttgccg atggtggaaa atacggcggt
     9061 ggacacggcg ataaaggcaa taatccattt gtttttattg cgttcgatag acgggctgag
     9121 accggagaag aacgacaccg aagtataagc cgcaccaatc accgaagtaa tcgaagccgc
     9181 ccaaatcacc acgccgaaaa tcagcaggcc gatgtatccc gccgcatatt caaacggtgt
     9241 ggaagcaggg ttgtcgggat tgagctgtac gccttggctg accacgccca aaaccgccaa
     9301 aaacaataca atccgcataa tcgaggcaat caggatcgcc cgcaccgagc tttggctcac
     9361 ttccggcaac gccgatttgc ctttgatgcc tgcgtccagc agacggtgcg caccggcgaa
     9421 ggtgatgtag ccgccgaccg tgccgcccac cagtgtaaca atcgccattg catcgagttt
     9481 ttccggcata aaggtatgca cggcggcatc tgccagcggc ggattcgcct gccatgccac
     9541 ataaaccgtc agcgcaatca ttacgaaacc catcacttgg gcgaatttgt ccatcacttt
     9601 gcctgcttct ttaaacagaa acacaccgat ggcaatcacg ccgctgatca cggcaccggt
     9661 ttccggtgac agtccggtca gcaggttcag acccaagcct gcgccgccga cgttgccaat
     9721 attgaacgcc aaaccgccca tcacaatcag cacagccaag aaatagcctg cgccgggcaa
     9781 gacctgattg gcaatatcct gcgcctgttt ttcggaaacg gcgacaatcc gccaaatatt
     9841 gagctgcgcc ccgatgtcga gcagaatcga gagcagaatc acaaagccga aacttgccgc
     9901 cagtgcttgg gtgaaggtgg cggtttgggt cagaaagccc gggccgatgg cggaagtcgc
     9961 catcaggaat gcagcgccga ttaaggcatt tctgcggttt ttttgattag acataatcgc
    10021 ttatcctcta taaaattggt tgttgctgtg tttgggcgaa acctgcggtt ttagctacgc
    10081 agaaactcgc tttgctcgtt ttggcgaaac ctgcggtttt cagacggcct atgaactgtt
    10141 tttcaagcag aaactttgat gcctgccgcc agtagttcct gccggatttt ttcggcaaac
    10201 accacggcgt gcggcccgtc tccgtgcaga cagatgctgt cggcttgcac ggcaaccagg
    10261 ctgccgttca ctgctttgac ccgcccgtcc cgcaccatct gcaatacttg ggcgatggct
    10321 tcttcgtcgc tgtccacctg cgcatcgggg cggctgcggg gaaccagcgt accgtcgggc
    10381 atatagcggc ggtcggcgaa tacttcggaa atcacaccca agcctgcggc ttttccggct
    10441 tccaagagca ggctgccgga aagtgccatc aatttcaatt tcgggtcgaa atccgccaca
    10501 attcgggcaa cggtatccgc cagcgcacgg tttttcgccg cttggttgta cattgcgccg
    10561 tgcggtttga cataagccat ttccaaaccc tgatcacggc acaaggcctg caatgcgccc
    10621 aactggtaat tcagacacgc ccgcaaatcg gcttcggaca gattcatttc ggtacggccg
    10681 aagttttccc gatcgggata gccggggtgt gctccgatgc gcacgccgtt ttgttgggca
    10741 tacgccaatg ccgcccgaat atcggcaatg ctgccggcgt gttgggcgca ggcgatgttg
    10801 gccgaagtaa tcagctgcaa caaggcttcg tcgctgccgc agccttcggc gagatcggcg
    10861 tttaaatcaa cctgcttcat gggtgattct ccgtatttgg ttcagataga cttggttttt
    10921 gcgccgcagg gcggtggctt ctttcaagcc gattattttg aatttgactt tgctgccgaa
    10981 gcgcacctgt gccagcctgc ccaaatcggc ggcggcaacg gtagcgattt tcggataacc
    11041 gccggtggtt tgcgcatcgg ccagcaggat aatcggtttg ccgccgggcg gcacctgcac
    11101 ggttcctgcc tgaacagcgt gggacagcat ttccaaaggt tgcgacaggg tcagcggctg
    11161 tccgtcgaag cggtagccca tgcggttgct atcgctttgc agcgtccacg tttcccgttc
    11221 cagattcaga cgcccttttt cactgaaagc ggcatattcc gacgaaggaa caaggtggac
    11281 ggtatcggta aacggtatcg gggcaatgcc gactttggac aattcctgcg cacctttgcc
    11341 gatggggaga caatcacctt tttgcagcat tctgccctga tggccgccga aaccggcttt
    11401 caggtcggtg cttctcgaac ccatcacttc cggcacatca aatccgcccg ccacgcacac
    11461 atagccgtac atgccctgca cggcacgcac cattttcaag gtctgccctt tgcgggcggt
    11521 ataacgccaa tacgaataga ccggttcgcc gtccaattcc gcctgataca cggcaccggt
    11581 gagacaaaac ggcgtatccc gttcaaacac cagcattatc ccgcccaaag cgatttcgat
    11641 tgcggccgtg ccttcgtcgt tgcccaataa aatattaccc gccgccaaag caaccgtgtc
    11701 catcgcaccg gcatgaccga tgccgtaacg ccggtgtccg tagcgtccgg tatcctgaat
    11761 atgcgccggt gcctgcactg ccgaaacgtg aatcatggct caatcctttc tgcaacaaag
    11821 cggacttggt cacccgccgc cagcagggtc ggcggattca aatcggctcg gaacaagggt
    11881 aattcggttc tgccgataat ctgccagccg ccgggcgaag cgaacggata cacaccggtc
    11941 tgactgccgc cgataccgac cgaaccggca ggaacggacg ttctcggcac ggcacggcgg
    12001 ggcgtgtgca atgcttcggg caagccgccc agataaggga aaccgggctg gaagcccatc
    12061 ataaatacgg tataagtttg cgccgtatgg cggcggacga tttcggaaat aaccgtctga
    12121 tggaaagcag cgacttccgc caaatccggg ccgtattcgc cgccgtagca gacgggaatt
    12181 tccaccagtt tgccctgatg gtctgtaacg gcggtgtgtt cccacacata ttgcaattca
    12241 tcggcaagcg tcgccaaatc ggtatcgaaa cgggtgaaca cggtcagatt gttcatgccg
    12301 accaccactt cctcaatcct gtcgtgctgc ccgagcgcag cggcaaacgc ccacaacttt
    12361 tgctgtttgc ccagttcgga aggtgcattc agtcggcaga ccaaagcgga ttcgctgatt
    12421 ggtgtgatct ctattctcat ttgttgttca ttttggttat gttttaatga atctatatgc
    12481 aggggcggcg gtttgtcaat atcttctgtg ctgcatcatc aaaccgtcga ttggaaaagt
    12541 gctgccctgc cgctgcactt tttcagacga ccttaaaccg tttctattaa aatagcgcat
    12601 tccacttttc agacggcatc tctatgtttc ccgaccaatc cgcccccaac ctgctgcaag
    12661 gcttgaatcc cgaacaactt tccgccgtaa cctggccgcc gcaatccgcc cttgtgctgg
    12721 caggcgcggg cagcggcaaa acgcgcgtgc tgaccacgcg tatcgcatgg cttttgcaaa
    12781 ccggtcaggc aagcgtgcac agcattatgg cggtaacgtt taccaacaaa gccgccaaag
    12841 aaatgcaaac ccgactcggc gcgatgattc ccgtcgacgt ccgcgctatg tggctcggca
    12901 cgttccacgg cctctgccac cgctttctac ggctgcacca ccgcgatgcc ggtttgccgt
    12961 cttcctttca aatcctcgac ggcggcgacc agctttccct catcaaacgc ctgctcaaaa
    13021 gcctcaacat cgccgaagaa atcatcgcgc cgcgttcgct gcaaggcttt atcaacgcgc
    13081 aaaaagaatc cggtttgcgc gcttccgtct tgagtgcgcc cgatccgcac acaagccgca
    13141 tgattgaatg ctacgccgaa tacgacaaaa tctgccaacg cgaaggcgtg gtcgattttg
    13201 ccgaactcat gctccgcagc tacgaaatgc tgcaaagcaa cgaaatcctg cgccagcatt
    13261 accaaaaccg cttcaaccac attctcgtcg acgagttcca agacaccaac aaactgcaat
    13321 acgcctggct caaactcatg gcgggcggca acgcggcagt gtttgccgtc ggcgacgacg
    13381 accaaagcat ctaccgcttc cgtggcgcaa acgtcggcaa tatgaccgcg ctgatggaag
    13441 agttccatat caacgcgccc gtcaaactcg aacaaaacta ccgctctgtc ggcaacatcc
    13501 ttgccgccgc caatgccgtt atcgaaaaca acgacgaacg actcggcaaa aacctgcgca
    13561 ccgacgccga agcaggcgac aaaattcgct actactccgc ctttaccgac ctcgaagaag
    13621 cccggttcat cgtggacgaa accaaagccc tcgaacgcga aggctgggat ttggacgaaa
    13681 tcgccgtcct ctatcgcagc aacgcccaat cccgcgttat cgaacaaagc ctgttccgca
    13741 gcggcatccc ttacaaaatc tacggcggcc tgcgctttta tgaacgccaa gaaatcaaac
    13801 acgcgctcgc ctaccttcgc ctcgccgtta atcccgacga cgacaacgcc ctcttgcgcg
    13861 tcatcaactt cccgccgcgc ggcatcggcg cacgcaccat cgaaaacctt cagacggcct
    13921 caaacgaaca aggcatcacc ctttggcagg cagcctgcaa cgccggcgca aaagccacca
    13981 aagtcgccgc cttcgtccgc ctcatcgaag ccctgcgcaa ccaagtcgga caaatgcccc
    14041 tgcccgaaat catcgtcggc atcctcaaag acagcggcct gaccgaacac tacaaaaccc
    14101 aaaaaggtga caaccaagac cgcctcgaca acctcgacga actcgtcaac gccgccatcg
    14161 agttcaaacc cgaagacagc aactttgaaa tcctgcctga aaacatttca gacgaccccg
    14221 cctttcccat cctcgccttc ctaagcaacg ccgccctcga atccggcgaa aaccaagcag
    14281 gcgcggacga aaaagccgtc caactcatga ccgtccacgc cgccaaaggc ttggaattta
    14341 acgccgtctt cctcaccggc atggaagaag gccgcttccc cagcgaaatg agtctcgccg
    14401 aacgcggcgg cctcgaagaa gaacgccgcc tcatgtacgt cgccatcacc cgcgcccgca
    14461 aacgcctcta catcaccatg gcgcaacaac gcatgctgca cggacaaacc caattcggca
    14521 tcgtctcccg cttcgtcgaa gagatcccac ccgaagtatt gcactacctg tccgtcaaaa
    14581 agaccgccta cgacagctac ggcagcccgc gccaaaccgc cgcatccaaa gacaaaatca
    14641 tcgacgacta caaacagccc caaacctacg caggtttccg tatcggacaa aacgtccgcc
    14701 acgccaaatt cggcaccggc gtgattatcg atgccgcaga taaaggcgaa tccgcccgac
    14761 tgaccatcaa cttcggcaaa cagggcgtga aagagttgga taccaagttt gcgaaattgg
    14821 aagcgatgta aatttgaaat gcaggctgga tactcgtatc cgacaaaaat atttgacgcg
    14881 tctatcgttt ccgaaaaacc gctgttggaa aatgtcggat tcgagaatca gacctatggc
    14941 aaaaaactgt agcaaggaca aggcaaaagg ccgtctgaat ccggtaacaa caaattttgc
    15001 cgaaacctcc gccgtcattc ccgcgcaggc gggaatccag acctttcggc acgggaactt
    15061 atcggataaa aggtttcttt agattccacg ttctagattc ccgcctgcgc gggaatgacg
    15121 gacggtaaaa gccgggtata gatacctaac ccttgtttat agctgaaaat caataaggaa
    15181 gaacattatg tcccaagttt ttaaagattt tgatttgtcc tccgtatggg aatccgacag
    15241 ttgggcagat gaaaactaca aagaagcccc gtttacccct gaaattttgg ctgccgtaga
    15301 aagtgaactg ggctataaat tgccgcaaag ttttattgaa ttgatggcgg tacaaaacgg
    15361 cggaatattt gtcaaaaact gttttccgac cacacagaga aattcgtggg cgaaagatca
    15421 tgtgcaaatt tgcgaggtat cgggcatcgg ctttgaaaaa gaagggagtt tgtgcggcga
    15481 aacagggcaa aaactttgga tggaagaatg ggaatatccg cccatcggcg tgtattttgc
    15541 caacgacccg tcaggcggtc atgccatgtt tgccttggac tatcgggagt gtggaaaaga
    15601 cggcgagccg aaagtggtgt ttgtcgagca agaatcggat tttgaaatcg tcgaacttgc
    15661 ccccgatttt gaaaccttta tccgcagctt gcggcatgaa gatgagttta ttgacgagga
    15721 aatataaaac ggcggttgaa aaaccggaat catcaaggga aaacgggcga aataacggat
    15781 aatcgcttga atccgtaagg aaaacggttt ggtggaacgc gccatccaag acctttgcaa
    15841 agaaaaactg tctccgacag cattgacatt attaacagaa cttatcaagt ttggagctat
    15901 ctcaaatata attcggttat ccttttgtat ccattaaatc atatgcttca attaattgtt
    15961 gttctagctc ttgtaccaat tttgaattgc gaattcctga cacaatctca aattcttctg
    16021 catctatgca aacacctgca taaatttcaa taacaaggga acgcaataat tgaagctctt
    16081 ctcttgttaa agaaataata atgtcatcac ctgtgtaatt gattatattc ataataattt
    16141 tatttttgtt tgtcaaagta agttttgcct aaggttggtc taaatgcagt tccaccatct
    16201 tttgaatttg ggtctctgat tacaattgct ccagacttat catcccaaat tgctcttatg
    16261 tgtttggatt gtaatcttcg aattcccaag aaaaaaatcg taataagttt gaaagtgtca
    16321 aatcccaagt ttcttttgag caatattcta atattttatc aatttcactt ttaataatct
    16381 tatgatcaaa ctgttctaat attaatgcat tagaccaaaa aaaaccttct ttattacaat
    16441 gatgggaaat ccatttagga gaacaaatgc aaagtgaaaa aatagatgag ccttgttctc
    16501 cttcgattcc gatatccaaa tctatccatc tatggaaatt atctggaatt tcgggggtaa
    16561 atttttcaaa atcaatatca tataaattta tgctttttaa atccaattta atcattaggg
    16621 ctgtcctaga taaataggga aattcaaatt aagttagaat tatccctatg agaaaaagtc
    16681 gtctaagccg gtataaacaa aataaactca ttgagctatt tgtcgcaggt gtaactgcaa
    16741 gaacagcaac agagcctgac agcattgttt atacggattg ttatcgtagc tatgatgtat
    16801 tagatgtgag cgaatttagc catttccgta tcaatcacag cacacatttt gctgaacgac
    16861 aaaaccatat caatggaatt gagaattttt ggaacc
//
LOCUS       DAXHNE010000028        16607 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.028, whole genome shotgun sequence.
ACCESSION   DAXHNE010000028 DAXHNE010000000
VERSION     DAXHNE010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16607
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.028"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            169..1092
                     /locus_tag="ACFM4Z_000729"
     CDS             169..1092
                     /locus_tag="ACFM4Z_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6020799.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAKPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYAGALVVVGGAVTAAVGDRLFKRR"
     gene            complement(1157..2545)
                     /gene="fumC"
                     /locus_tag="ACFM4Z_000730"
     CDS             complement(1157..2545)
                     /gene="fumC"
                     /locus_tag="ACFM4Z_000730"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFC6020800.1"
                     /translation="MSTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAIGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYGNAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(2693..4672)
                     /gene="tkt"
                     /locus_tag="ACFM4Z_000731"
     CDS             complement(2693..4672)
                     /gene="tkt"
                     /locus_tag="ACFM4Z_000731"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFC6020801.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPDNF
                     DEYVQTALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLALHGGIKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLDDIKRGGYIISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQAAVLPESLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            5199..6695
                     /gene="ccoG"
                     /locus_tag="ACFM4Z_000732"
     CDS             5199..6695
                     /gene="ccoG"
                     /locus_tag="ACFM4Z_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HFC6020802.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     TAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            6698..7246
                     /locus_tag="ACFM4Z_000733"
     CDS             6698..7246
                     /locus_tag="ACFM4Z_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HFC6020803.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAIRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(7587..8762)
                     /locus_tag="ACFM4Z_000734"
     CDS             complement(7587..8762)
                     /locus_tag="ACFM4Z_000734"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HFC6020804.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(9321..9395)
                     /locus_tag="ACFM4Z_000735"
     tRNA            complement(9321..9395)
                     /locus_tag="ACFM4Z_000735"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9360..9362),aa:Glu,seq:ttc)
     gene            complement(9413..9489)
                     /locus_tag="ACFM4Z_000736"
     tRNA            complement(9413..9489)
                     /locus_tag="ACFM4Z_000736"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9453..9455),aa:Arg,seq:acg)
     gene            9607..10992
                     /locus_tag="ACFM4Z_000737"
     CDS             9607..10992
                     /locus_tag="ACFM4Z_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFC6020805.1"
                     /translation="MIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYEDRVTEVALV
                     KFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELFSVLKGCVLV
                     AHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSIIERLGIVVED
                     RHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLREQLYGLPDG
                     MGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFPALGSLHAYK
                     VKAELVGRYHSDCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVTDNPPTGLFA
                     NKKAARRALSSWAETYGLCPAAAGILPDGYAEDEPCPVYVSGRCDKACGRSDEQVLAF
                     AHKLPVLDWGKMHEVEITETDPLTGEKVVLHGMGGALEMDDGLWYFDKDLPDAFKAKF
                     KTDRKNIKEIG"
     gene            complement(11203..11982)
                     /locus_tag="ACFM4Z_000738"
     CDS             complement(11203..11982)
                     /locus_tag="ACFM4Z_000738"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC6020806.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGNTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALTEHPLVGEYGHSFRFVPVTTRAANPSGLSGKRIPELLKNNSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            12256..15345
                     /locus_tag="ACFM4Z_000739"
     CDS             12256..15345
                     /locus_tag="ACFM4Z_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFC6020807.1"
                     /translation="MNQNHFSLKILTVMLLSAYGGSFADGVVPVSDGNTVSLDTVNVR
                     GSHALLGKTEKTRSYTIDRMSTATGMRIAGKDTPQSVSVITRSRLDDKAVHTLEEAMK
                     NTTGVNVVRDSGLQTRFLSRGFYIDQIGEDGMTVNVAGRSGYTAKIDVSPSTDLAVYD
                     HIEVVRGATGLTQSNSEPGGTVNLIRKRPTASFKHTGELTADHRGSRRAVLDVSGSLN
                     KANTLRGRLVGAEEYKKSFKDRVWGRKHMVYGIAEADAGDSSVLTLGGMYQKSREVPD
                     FSGIILPCENQKTAPFSSTPACNRPLQLPRNTYLGEDWSRLSADKYNLFSGFKHVFDN
                     GWQLNAEVSYTKNESDAKVGQFFLKNEYAAGLSGEDAVGFLTEKNEVIPFEPKDKALE
                     KLKAYRDETAKEYRERKDDFVKNRFDNTAFEQYRSRRAAERKAGFDKCMSDPFALDFI
                     CQGSWGDPGVDADKAEFVDKALAKEGIFNNAAQRFPNSLYDSSFNRKATANRRYSYMP
                     LRHTKDDRQWGIKLDLTGTYGLFGREHDFFVGYAYGDEKIRSEYLEIYERRYRVRPNT
                     GATHGVYAGSCQEEPDGDLSSPLVRGHKEPDWQAYDEKGNRTVYAEECRNAKKIKTEP
                     KLDAEGKQVYYYDEYSGSRTPVYVDVYELDEKGNKIQETNPDGTPAFTGFSGTVPVWK
                     TVKVADDHVPALYNYAKYLNTNKTHSLTASTRFNVTGRLHLLGGLHYTRYETSQTKDM
                     PVRYGQPASDFQTASSIRADQDHYTAKMQGHKLTPYAGITYDLTPQQSIYGSYTKIFK
                     QQDNVDVSAKTVLPPLVGTNYEVGWKGAFLQGRLNASFALFYLEQKNRTVVDFGYVPG
                     AGGKQGSFQTVAKPIGKVVSRGAEFELSGELNEDWKVFAGYTYNKSRYKNAAEVNAER
                     LAKNSSADPYNFSNFTPVHIFRFGTSFHIPNTGLTVGGGVSAQSGTSSLYNIRQGGYG
                     LIDGFVRYELGKHAKLSLIGTNLNGRTYFENNYNRTRGANNFYGEPRTVSMKLDWQF"
     gene            15489..16547
                     /gene="dinB"
                     /locus_tag="ACFM4Z_000740"
     CDS             15489..16547
                     /gene="dinB"
                     /locus_tag="ACFM4Z_000740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFC6020808.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
BASE COUNT         3827 a         3882 c         4753 g         4145 t
ORIGIN      
        1 aaaattcagg cggtgttttc aaatagcgcg aagcaatagc cgagcttcag ccggtttgtt
       61 tgaaatcaaa gggttggaat caaacgccaa acgcttgaac catcattgcc cgatgcggca
      121 aaccggcatc gtccggcaat acggttgttt aaaaatagga aaatcaggat ggaaaaccaa
      181 aggccgctcc taggcttcgc gttggcactt ttggcggcga tgacgtgggg aacgctgccg
      241 attgccgtgc ggcaggtatt gaagtttgtc gatgcgccga cgctggtgtg ggtgcgtttt
      301 accgtggcgg cggcggtatt gtttgttttg ctggcactgg gcgggcggct gccgaagcgg
      361 cgggattttt cttggtgctc attcaggctg ctgctgctcg gcgtggcggg catttcggca
      421 aactttgtgc tgattgccca agggctgcat tatatttcgc cgaccacgac gcaggttttg
      481 tggcagattt cgccgtttac gatgattgtt gtcggtgtgt tggtgtttaa agaccggatg
      541 actgccgctc agaaaatcgg cttggttttg ctgcttgccg gtttgcttat gttttttaac
      601 gataaattcg gcgagttgtc gggtttgggc gcgtatgcga agggcgtgtt gctgtgtgcg
      661 gcaggcagta tggcatgggt gtgttatgcc gtggcgcaaa agctgctgtc ggcgcaattc
      721 ggaccgcaac agattctgct gttgatttat gcggcaagtg ccgccgtgtt cctgccgttt
      781 gccaaaccgg cacacatcgg aagtttggac ggtacgttgg cgtgggtttg ttttgcgtat
      841 tgctgcttga atacgttaat cggttacggc tcgttcggcg aggcgttgaa acattgggag
      901 gcttccaaag tcagcgcggt aacaaccttg ctccccgtgt ttaccgtaat attttctttg
      961 ctcgggcatt atgtgatgcc tgatactttt gccgcgccgg atatgaacgg tttgggttat
     1021 gccggcgcac tggtcgtggt cgggggtgcg gttacggcgg cggtggggga caggctgttc
     1081 aaacgccgct agttcgcagg caacggaaaa tgccgtccga acgaggtttc agacggcatt
     1141 ttatttgagg gaaagattag cgcggatgaa ccatatcggc aggaacgacc agttcgtcaa
     1201 attcttcgcc cgtcagcaag cccaactcaa cggcggtttc gcgcaacgat ttgttgtttt
     1261 tgtaggcggt tttggcgact ttggcggcgt ttccgtaacc gattttacgg tttaacgcgg
     1321 taacgagcat cagggaatgg tgcaggaaat agtcgatttt ttccggtacg ggttcgatgc
     1381 cgatggcgca gtgttcgttg aagctgttgc acgcgtcgcc caagaggcgg atggattgca
     1441 agaggttgta ggcgataacg ggcatataga cgttcagctc gaaattgccc gacgcgcccg
     1501 ccatgccgat ggtaacgtcg ttgccgaaca cttggcagca caccatggtc atcgcttcgc
     1561 attgggtcgg gttgactttg cccggcatga tggacgaacc cggctcgttt tcggggattt
     1621 tgatttcgcc caaaccgcag cgcgggccgc ttgccagcca gcggatgtcg ttggcgattt
     1681 tattcaggct tgccgccagc gttttcaatg cgcccgaagc ggcaacggcg gcatcgcgtc
     1741 cgcccaaggc ttcaaatttg ttcggcgcgc tgacaaacgg caagccggac aattcggcga
     1801 gtttggcggc ggctttttcg gcgtattcgg gatggctgtt caaacccgtg ccgaccgccg
     1861 taccgcccaa agcaagttca tacaagtctt taagcgcgtc gttcagacgg cctaagccgt
     1921 ggtcgagctg ggaaacgtag ccggaaaatt cttggcctaa agtcagcggc gtcgcgtcct
     1981 gcaagtgggt gcggccgatt ttgacgatag gggcgaaagc ttgggctttt ttgtccaagg
     2041 tatcgcgcag ggcttttacg gcggggatga ggtggcggtt gatttcaatc gcggcggcaa
     2101 cgtggatagc ggtcgggaat gcgtcgttgg tcgattgcgc gtggttcaca tggtcgttgg
     2161 gatggacggg ctgataagcc gccaaacccg taccggcgat ttcgttggcg cggtttgcca
     2221 gcacttcgtt catgttcata ttggactgcg tgccggagcc ggtctgccaa accactaaag
     2281 gaaactgtcc gtcaagcctg ccgttcaaca catcatccgc cgcctgcgta atcaaatccg
     2341 cctgttccgg cttaatcctg ccgagggaaa cattggtcgc ggcggcggct tttttcacca
     2401 gcgccaaagc ataaatcagc ggctgcggca gggtttcgcc gccgattttg aaattgttgc
     2461 ggctgcgctg ggtctgcgcg ccccaatagg cttcggacgg gacttcgaca ttgcccatgg
     2521 tgtcgtgttc ggtacgggtg ctcatgcgtt tctccttttg aaatgtgaat aagagtgatt
     2581 cgcaaatatt ataatggaga ttggaggaat gaggaagtat caaggagaga gggcaaacaa
     2641 atgccgtctg aagccctaat tggtttcaga cggcatttgt ttaggtgtgg ggttacagca
     2701 cggatttcac cgtatcaacc acattgtcca cggtaaagcc gaatgctttg aagagtaaat
     2761 cggcaggggc ggattcgccg aagcggttga tgccgacgac tgcgccgttc agtccgacat
     2821 atttgtacca gccgtcggcg tggccggctt ctacggcgat gcgcggcagg ctttcgggca
     2881 ggacggcggc ttgataggcg gcgtcttggc ggtcgaatac gttggtggac ggcatggaaa
     2941 cgacgcgcac ggcgatgttt tgcgcggcga gggctttttg cgcttccaaa gccagctcga
     3001 cttctgagcc ggtggcaatg atgacggctt gggcgttgcc ttgggcttcg ctgatgatgt
     3061 agccgccgcg tttgatgtcg tccagttgtt gttcgctgcg cgcttggaat ttcaggtttt
     3121 gacggctgaa aatcaggcag gacgggtgat cggcggcttt gacggcttct gcccaagcga
     3181 ccaaggattc ggcggtgtcg cacggccgcc atacgtccat attcggaatc aggcgcaggg
     3241 tggcggtttg ctcgatgggt tggtgggtcg gaccgtcttc gcccaaaccg atggaatcgt
     3301 gggtgaacac aaatacaggg ttgattttca tcagtgccgc catacgcagg gcgttgcgct
     3361 cgtattcgct gaacatcagg aaggtcgcac cgaatggttt gatgccgccg tgcagggcaa
     3421 gaccgttcat aatcgcaccc ataccgaact cgcgcacgcc gtagtggatg tagttgccgc
     3481 ctttgtcgcg ggtaacggag acgctgtttg accagtcggt caggttggac ggggtcaggt
     3541 cggccgaacc gcccaccaat tcaggcaact cttttgccaa gatttcgatg ctgttttggc
     3601 tggctttgcg ggtagcgatg gtttcggctt tggcgcacac ttctttcaat gccgtctgaa
     3661 cgtattcatc gaaattgtcc ggcagctttt tatccatacg gcgcacaaat tctgcggctt
     3721 cggcaggata tttggcttga tattgcgcga acagttcgtt ccagtcggct tccagtttcg
     3781 cgccttgttc tttggcattc cacgcatcgt aaatttcttg cgggatttca aaggcggggt
     3841 aagtccagcc caaatgtttg cgcgtggctt cgatttcgtc cgcgcccaaa ggtgcgccgt
     3901 gggttttgtg gctgccttct ttgttggcac tgcctttgcc gattaaggtt ttgcagcaga
     3961 tgatggacgg tttgccggtt tcggcacgtg cggcttcgat ggcggcttga atggcggcgg
     4021 tgtcatgacc gtttacgtta ggaacgacgt gccagccgta gctttcaaag cgttgcggga
     4081 tgttttcggt aaaccagccg tccactttac catcaataga aatattgtta tcgtcataca
     4141 aaacaatcag tttgcccaag cctaaagtgc cggcgagcga acaggcttcg tgcgatacgc
     4201 cttccatcag acagccgtcg cccatgaaga cgtaggtgta atgatcgacg atgttcaaac
     4261 cgtctttatt aaattcggcg gcaaggattt tttctgccaa tgccataccc accgcgttgg
     4321 caatcccttg ccccaacggg ccggtcgtgg tttccacgcc gtcggtgtag ccgtattcgg
     4381 ggtggccggg ggttttgctg tgcagctggc ggaagttttt caagtcttca atgctcagat
     4441 tgtagccggt caggtgcagc aggctgtaca acagcataga cgcgtggccg ttggagagga
     4501 cgaagcggtc gcggttgtag aatttggggt tggcggggtt gtgattgagg aatttcgtcc
     4561 acaatgtttc cgccatttcc gccataccca taggcgcgcc ggggtggccg gaattggctt
     4621 tttgaacggc atcggccgag aggaagcgga ttgcgtttgc cagttgagac attttgtatt
     4681 ttccttgctg gtgtttcaga taagtggata atcggaaggc gttgattatc gcccgattcg
     4741 cttatgcttt caagaaaagg gcggacgcgg gggaaggcgg cggcagggga ccggtgggga
     4801 aggattttcg atttgcgggc gaagcctgcc attattcctt ttgaaataaa atgttataga
     4861 ttgtgtgccg gattgtcgat agcgtttgtt tatgagcttg cgccgtcggt tctgccgata
     4921 tgggggtgtc ggttttttta gtcttttttt taaccgtatt cggattttgt tcgggcggta
     4981 aggtaaaatc caggcgtttt gatgcggatg ggaaatgtat ccgccccata catccagacg
     5041 gcgcataaag ttgtacaata gcggaaatat attttgggaa aaacggattt tctcaaaact
     5101 tgaaacacaa cgccttaaaa aacaagaaaa atgacgtctg aaaagcaaac ggcggcggta
     5161 acgggcaaac catcaatcaa aaatttaagg aatgcagaat gaccacggaa aaccaagccg
     5221 gcagtccggc atccggaatc ggcacgtccg aacaaacaaa agccgcgccg aaagcaaaaa
     5281 aaacattcga tccgcgagcc agcgtgattc aaatccatcc cgaaggcgaa cgcatccatc
     5341 ccaaaaaggc ggaaggacgg ttcgccaaac tgcgtatcgc cgccgtattg gcgacgcagt
     5401 ttgtgtttta cgtcattccg tggttcaact ggagcggcag gcaggctgtc gttttcaata
     5461 tccccgaacg gcatttcttc attttcggat tgtcgttggg ggtgggcgat ttgatttacc
     5521 ttgccttgct gctgatgatt tgcgccttcg ggctgttttg gtggacgacg attgccgggc
     5581 gactgtggtg cggctattcc tgcccgcaaa cggtttacac cgaaattatg ctgtggatcg
     5641 acaacctggt cgaaggcgat agaaacaaac ggctgaagct ggaaaaatcg ccgtggaatt
     5701 tcactaaaat ccgcatcaaa gccaccaaat acctgttgat tttccttgtc tgcgcgtgga
     5761 cgggcatcac gtttgcaggc tggtttgtcc ctatccgcca gttcgttccc gatttattca
     5821 ctggagcagc aggcggcggc gcgatgtttg ccacagcgtt ttacggcttt atgaccttct
     5881 tcttcgccca cattatgcgc gaaaaagtat gcctgcatat gtgtccgtat gcacgtttcc
     5941 aaagcgcgat gttcgacaag gacacgctga ttgtttctta tgacgcggaa cgcggcgaac
     6001 cgcgcggcgc gcgcaagaaa acggtcaaca aggaagaggc gggtttgggc gactgcatca
     6061 actgtgcgat gtgcgtccaa gtctgccctg tcggcatcga catccgcaac ggtctgcagt
     6121 accaatgtat cggctgcgcc gcctgtatcg acgcgtgtga tgagattatg gacaaaatgg
     6181 gctatccgcg cggattaatc cgttatacga ccgaaagcgc gctggaacac gaatattctg
     6241 aaaaagacat taaaaaacgg ctgctcagac cgcgtgtggc aggttacggc gcggtgttgg
     6301 cgttagttat cattgccttc ctggccggtt tgtccacgcg caaaatggtc gaggtcgata
     6361 ttttgaaaga ccgtggcgta ctggtgcgcg aaaacgccaa aggctggctg gaaaacgcat
     6421 acagcctgcg tatcatcaac aaaagtgaaa aagaacagct cattaccgca agtgtcaaag
     6481 gctttgacga aatcgccctg accgggctgc ccgaaggcgg tatcaaggtt gccccgcgcg
     6541 aaacggtaac ccttcccgtc caagtgtcca ccattccgga atacgcggac aaaggcagcc
     6601 accctatcga atttaccttc caataccgcg aaagcggcgc gcccgacggc aagccggtcg
     6661 tcttggaaga agatgcaacc tttatcggag aataaccgtg tctcaaaaca ctccaatcaa
     6721 accttggtac aaacacgtct ggccgtggat cttgatggcg gggccgattt ttgtcgtcat
     6781 cgccagcgtc gctatgtttt ttgtcgcgca gcagcacgcg acagatttgg ttacggacga
     6841 ttattataaa gacggcaaac atatcgacat ccagcttcat cgggatgaag aagccatcag
     6901 acggcatatc ggggtgcagg ttctcatttc ccccgatatg aatgcggcaa aagtgtttgt
     6961 cggcggcgag tttgacggca aacagccttt gaacctgctg ctgatgcacc cgacccgcaa
     7021 ggcggacgat caaaccgtcg ccctcaagcc cgtcggcagc gcgcagaacg gcagggcgga
     7081 atatgaggcg gtgttcaaaa ccctttcgcc gaccaaccac tggtatgtgc gcgtggagga
     7141 cgcggcaggc gtgtggcgcg tcgagaacaa atggattacc agccaaggga atgcggtgga
     7201 tctgaccccg atggataaac ttttcaataa tgccggaagc aaataaaaaa gccgtgtttt
     7261 cattgcattg gtttgccaga tgcaatgtcg ggcaggcatt gtttgattat tcggcgtgat
     7321 ggtctgattt atcggacgaa aataaatgta tcttatcccg tttatttttt aacaccattc
     7381 cgaaatgcag cctgaaaaat gccgtctgaa gggcttcaga cggcatttgt gttgtatgcc
     7441 cgacacaatg cggaaggcgt tttgtttaca taaaaacgtt tttatttctc tccgtttttt
     7501 cgacactatt ccgaaacgca gcctgaaaaa tgccgtctga aaggcttcag acggcatttt
     7561 gtttgcaaat caaatcctac ctgatgtcag cgtccgagcg ttgcaacagg ttcgctcccc
     7621 gtcagcagat attgtatcgt ctcgcggacg gtgcggatgg cattgccgaa gggcagcggg
     7681 tctttacctc tgaagaacag gcctttatct acttctccac ggaatgcggc ggcaagctgg
     7741 atatcaatgc agaattgtcc ggctttggaa agcccgtcgc gcagaccgca actggttagg
     7801 cagtttaaac cttgggtaca gcggcgcggg tcggctttgg cgtttgtctg aagtttgctt
     7861 tcacgcttga tgtagctgtc taggaatttg gtgcggacac cgcgcgccgg caaaccggca
     7921 acagacataa attcgactac tttttcagtt tccgcaccgg cgagcgtttt tttgaagtta
     7981 aggtgtgcat ctccttcttc ggtaacggca aaagccgtac cgatttgaac ggcggatgct
     8041 ccccagttct ttagggcggt tttgactttt tcaaaatttg ccatgcctcc cgcaagaata
     8101 agcgggattt tttcgctttc cagccctaaa cttttgaaaa cttcaaacgt ttcctcaatc
     8161 acgcgtttga agtcgaattt agcatcgttt acgccttcga cggttgacgc gcccaaatgc
     8221 ccggccgcgt gggcaggatg ttcgactaca atcgcatcgg gcaatatgcc ttttttcatc
     8281 caacgtttca agacgatatt aataccgcgc gattcggaca gaatcggcag cagcgcgaca
     8341 tctttatgat agccctcggt catttccggc aggtctaaag gcaggccggc acccattaca
     8401 accgcatccg cccctgattc gcaagcctgg cggacatacg cggcgtggtc tttgaccgcc
     8461 ttcatcacgt tgaccgcaat cagtcctttt ccctctgaag cgcttttggc tttttggatt
     8521 tccctgtcta atgcggtaca gttcaaagat gtatatttct cttcactcgg attgatttgt
     8581 gattcggcga gtaggtcttc gtgaaggtgg cgcaaatcca cactggcaat cgttccgata
     8641 ccgttttcac gtgccaccgc gctggataaa cccgatgcgg aaacaccgac ccccataccg
     8701 ccttgcacga tgggggtaag ggattttcca cgaataacca aagggtcaaa aatattttgc
     8761 atcagtttct ccgagtatac gaatcagtca ggctcatgaa aaatggttct aactgtttta
     8821 gaactattgc tcaaatttgg tattatactc taaatatcgc ggcggtaaac aagaaagcag
     8881 ccggagcttc gggcatattc tgttacgcct gtttcaatcg ggagagaaaa tattatagtg
     8941 aattaacaaa aatcaggaca aggcgacgaa gccgcagaca gtacaaatag tacggaaagg
     9001 cgaggcaacg ccgtactggt ttaaagttaa tccactataa atttgatgat ggcataaaaa
     9061 agccctagtc agttgctgtc aaaggggatt gttaagaaaa gtaatgccac gttgatgggg
     9121 tgcaatatat caggcgtttc aatcgggtaa aatgttggac ggacaccgca tccggttacg
     9181 tgatatcggt ttgcacggca aaggtttgaa tatgattcga ttacgtctga gagtctgatg
     9241 aagggggtag gtaaagatat ggtaatgaat tgccgaggca ataaaaatcc ccgagggatt
     9301 atttcggggg atttggaatt tggcacgccc acggggaatc gaaccccggt taccgccgtg
     9361 aaagggcgat gtcctaaccg ctagacgatg ggcgcatatt gtattttctt actggcgcac
     9421 ccggagcgat tcgaacgccc gaccctctgg ttcgtagcca gatactctat ccaactgagc
     9481 tacgggtgca ctgcaagaaa gatttgaatt ttagggtaat catcggattc tgtcaattgt
     9541 tttgttggat gaaatgcctt aaaagtgtaa aatatcggtt tgttgtttga atttaaagag
     9601 aataaaatga ttgttgcttc ccgctggccc ttgctggaaa aggtattttt acggtttgga
     9661 atgcctgttg ccgtggtcga tttggaatcg acgggcggca atctgtatga agacagggta
     9721 accgaagtgg ctttggtcaa gtttgagcag ggaagggtgg tgaggcatga gtggttggtt
     9781 aatcctcaaa aaccgattcc gcagtttgtg gcggggctga cggggatttc agacggcatg
     9841 gttgccgatg cgcctgtttt tgcagagatt gccggcgagt tgttttcggt attgaagggt
     9901 tgtgtgctgg ttgcacataa cagccgtttc gactatacgt ttttaaagca tgagtttcat
     9961 cgtgcgggta tcggattttc atcgcctgct ttgtgcagtg tgcagctgtc ccggtgtctg
    10021 tatccgcaat tttacaagca cagcctggac agtatcatcg aaaggttggg gattgttgtg
    10081 gaagacaggc atcgtgcgat ggcggatgta tcggcattgt gtgattattt ggaatacagt
    10141 ctgtcggaac acggggttga ggcatggatc aggcagtgtt tccgtttgat gaatccgaaa
    10201 ccgctgcctg ccgcgctgcc cgaacggttg agggaacagt tgtacggttt gcctgacggt
    10261 atgggggtgc tggcttgttt cgacggcgga gggaaagtaa attatatcgg tacgtttgaa
    10321 cgggtatata gcgaggtttc ggctttattg gactccggaa aagccccgtt tgattggtgc
    10381 aatacggagg aagtccgttt ttttcccgca ttgggcagcc tgcatgcata taaggttaaa
    10441 gcggaattgg tcgggcgtta tcattcggat tgttatgtgt ctgccaaaaa tctgcttaaa
    10501 acatttacga ccgtcaggtt tgaaaaaggt tcagacggca tgttgaatgc gaaaacagcg
    10561 gctttgaaaa acggtgtgac ggataatccg cctaccggat tatttgccaa taaaaaggca
    10621 gcgagacggg ctttgtcgtc gtgggcggaa acatacggat tgtgtcctgc cgcagccggt
    10681 atccttccgg acggttatgc agaggatgag ccttgtcccg tatatgtttc aggccgatgc
    10741 gataaggcgt gcggccgttc tgacgaacag gttttggcgt ttgcacacaa gttgcctgtt
    10801 ttggattggg gaaaaatgca tgaggtggaa attaccgaaa ccgatccttt gacgggggaa
    10861 aaagtcgttc tgcacggaat gggcggcgca ttggaaatgg atgacgggct ttggtatttc
    10921 gataaagatt taccggatgc gtttaaggcg aagtttaaaa cggacaggaa aaatattaag
    10981 gaaatcggtt aaatcgatat cggttggtga aagccggcaa gtatgtaaac agccgtcacc
    11041 gtgttgattg cctaatcggt ttgtgccggt gtgttgccgt ttgcaggtaa tgtatccgaa
    11101 gatgccgtct gaattttttc agacggcatt tttgccgacg cggttttcgt gccgtgtcgg
    11161 tttgcccgtc catccgaagg catcggacaa gcagggtggt ttttagaagc cgttttccat
    11221 catgatttga ccgggaatgc ggttgcggtg catggcgtaa cccatgtcga gcagcgtttg
    11281 gaaagtgtct ttgaccattt ccgggttgcc gcaaatcata aaccgtgtgg attccggggt
    11341 gaacttggta tgcagcgcct gttcgatgct gttgtttttt aagagttccg gaatgcgttt
    11401 tccgcttaag cccgaggggt tggcggcacg ggtggtaaca gggacgaaac ggaaagagtg
    11461 tccgtattcg cctaccaggg gatgttcagt caatgcggcg agtcggtcgt tgaaaatcaa
    11521 ttcttcggga aaagatacgg aatgtatcag gttgacggta tcgaaacgtt gacggatttc
    11581 gggttgttcg agaatggaaa ggaagggggc gatgcccgag ccggtgcaga gcatcaccaa
    11641 atccttgccg tcggggaagc gttcgggcag gaggaagccg gtggcatttt tatcgagcag
    11701 gatggtgttg ccctgttgca ttttggcgaa acgggccgac atggggccgt cttggatgag
    11761 tacggcaaaa tattcgagcg tgtcggcata ttctgcggaa acaatggaat aggcacgcca
    11821 aatgaaacct tccccttcgt agaaaccgag tcgggagaac tgtccggctt taaagcggta
    11881 ggattcggga cggctgatgg cgaaagtgat gagtttcggc gtgtggtgtt tgacccacaa
    11941 aatcttttct tcggtgaatt ttgcttcggg cgaggctgcc atgttgttgt ccttgagtgt
    12001 gtttgtcggg attatagcca atttgggtgt ttttttctgc attggcggtc tgtatttaaa
    12061 ttcggatatt tttcaataca atgttatcgt tttttgaaat aaacggtaag gagtagtttg
    12121 ttttttgtca aaatgggaat acaatcctat ctataaattt aattgagaat tacttttttt
    12181 caattaatat aaaaaatcaa aacagttgtg agagcctttg gacgatttgc acacttgcat
    12241 attacagagg tctctatgaa tcaaaatcat ttttcactta aaattctgac cgttatgctg
    12301 ttatcggctt acggtggttc ttttgcagac ggtgttgtgc ctgtttcaga cggcaatacc
    12361 gtcagtctgg atacggtcaa tgtacgcggc tctcatgctt tgttgggcaa gaccgaaaag
    12421 acccgttctt atacgataga tcggatgtcc accgccacag gtatgaggat tgcgggcaag
    12481 gatacgccgc agtcggtcag cgtcatcacg cgcagccgcc ttgacgataa ggcggtgcat
    12541 acgcttgaag aggcaatgaa aaacacgacg ggtgtcaacg ttgtgcgcga ttcaggcttg
    12601 cagacgcggt ttttgtcacg cggtttctat attgatcaga ttggtgaaga cggtatgacc
    12661 gtcaatgttg caggccgttc gggatatacg gcgaaaatcg acgtgtctcc gagtaccgat
    12721 ttggcggttt atgaccatat tgaagttgta cggggtgcaa cggggttgac ccaatccaat
    12781 tcagagccgg gaggaaccgt caatttgatc cgtaagcgac ctaccgcttc tttcaaacat
    12841 accggagagc tgaccgccga ccatcgcggc agccggcgtg cagtgttgga tgtttcaggc
    12901 agcctgaata aggcgaatac cttacgcgga aggttggtgg gtgcggaaga gtataaaaaa
    12961 tcgtttaaag accgcgtttg gggcaggaaa catatggttt acggcattgc cgaagccgat
    13021 gcgggcgaca gcagtgtgct tactttgggc ggcatgtatc agaagagtag ggaggttcct
    13081 gatttttcgg gcattatttt gccctgtgaa aatcagaaaa ctgccccgtt cagttcaacg
    13141 cctgcctgca accggccttt gcaactgccg cgcaacactt atttggggga ggattggtcg
    13201 cggttaagtg ccgacaaata caaccttttc tcaggattca aacatgtgtt tgacaacggt
    13261 tggcagctca atgccgaagt gtcttatacc aagaatgaat ccgatgcgaa ggtggggcag
    13321 ttttttctga aaaacgaata tgcggcgggt ttgtcgggtg aggatgcggt aggctttttg
    13381 actgaaaaaa acgaagtcat cccgttcgag ccgaaagata aggcattgga gaaactgaaa
    13441 gcatatcgtg atgaaaccgc caaggaatac cgggagcgca aagacgattt tgttaaaaac
    13501 cgtttcgata atactgcttt cgaacagtat cgcagccgcc gtgccgcaga acgcaaagcc
    13561 ggttttgaca agtgtatgag tgaccctttc gcgctggact ttatctgtca aggttcttgg
    13621 ggggatccgg gcgttgatgc cgacaaggcg gaatttgtcg ataaagccct tgcgaaggag
    13681 ggcatcttta ataatgcggc acaacgtttt ccaaacagcc tgtatgactc ttcctttaat
    13741 cggaaggcta ccgccaaccg acgatacagt tatatgccgt tgcggcatac caaagatgac
    13801 cgccaatggg gaattaaact tgacctgacc ggcacatatg ggctgttcgg gcgggagcat
    13861 gatttctttg tcggctatgc ctacggtgat gaaaagatac gttcggaata tctagaaatc
    13921 tacgaacgcc gctacagagt acgtccgaat acgggggcaa cgcacggcgt gtatgcggga
    13981 agttgtcagg aggagccgga cggcgatttg tcgtctcctt tggtcagggg gcataaagaa
    14041 cccgattggc aggcgtacga tgaaaaaggc aaccgtaccg tttatgccga agaatgcagg
    14101 aacgccaaga aaataaaaac cgagcccaag ctcgatgccg aaggcaagca ggtgtattac
    14161 tatgacgaat acagcggcag ccggacaccg gtatatgtcg atgtatatga gctggacgaa
    14221 aaaggcaaca agattcagga gaccaatccc gacggcacgc ctgcctttac cggtttttcc
    14281 ggtacggtgc cggtttggaa aaccgtcaaa gtggcagacg accatgttcc tgcgctgtat
    14341 aactacgcca aatacctcaa caccaacaaa acccattcgc tgactgccag cacgcgtttc
    14401 aacgtaaccg gccgactgca ccttttgggc gggctgcact acacgcgcta tgagacttcg
    14461 caaaccaaag atatgcctgt ccgctatggg cagccggcaa gcgattttca gacggcatcg
    14521 agcattaggg cggatcagga ccattacacg gccaagatgc aaggtcataa attgacgccc
    14581 tatgcaggca ttacctatga cttgacaccg caacagagta tttacggaag ttataccaaa
    14641 atcttcaaac agcaggataa tgtcgatgtc agtgccaaaa ccgttttacc gcctttggtc
    14701 ggcacaaact atgaggtagg ctggaaaggc gcgttcttgc aaggacggct gaatgcttcg
    14761 ttcgcattgt tttacctcga acagaaaaac cgcacggtcg tcgatttcgg ctatgttccc
    14821 ggagcaggcg gcaagcaggg gtcgttccaa accgttgcca aaccgatagg caaagtggtc
    14881 agcaggggtg cggaattcga gttgtcgggt gagttgaacg aagattggaa agtctttgcg
    14941 ggttacacct acaacaagag ccgctacaaa aacgccgccg aagtcaacgc cgaacgcctt
    15001 gccaaaaatt ccagtgcaga cccgtacaac ttcagcaatt tcacacccgt gcacatattc
    15061 cgtttcggaa cgagcttcca tataccgaat acggggctga ccgtcggcgg cggcgtgtcc
    15121 gcacaaagcg gcacaagcag tctgtataac atcaggcagg gcggctacgg gctgatagac
    15181 ggtttcgtcc gttacgaatt gggcaaacac gccaaattga gcctcatcgg tacgaactta
    15241 aacggacgca cttattttga gaacaactac aaccgtacgc gcggcgcaaa caacttctac
    15301 ggagagccgc gcactgtcag catgaaactg gattggcagt tttaattgaa aaccgtagtt
    15361 ttgcggatgc cgtctgaaac agggtatgtt tcagacggca tttttatgga ggcaggtcta
    15421 tcggggcgta tatttggaat ttgcccgatg ccggcagtag aatatccctt ttatcctcag
    15481 aagagccgat gtcttcacgc aaaattatcc acatcgacat ggacgcattc tacgcatcgg
    15541 tagagctgcg cgaacagccg catttgaaag ggcggccggt ggtcgtcgcg tgggagggcg
    15601 cgcgttcggt gatttgcgcc gcatcgtatg aggcacggca gttcgggctg cattccgcga
    15661 tgtcggtggc aacggcgaaa aggctgtgtc cgcaagcggt gtatgtgccg ccgcatttcg
    15721 atttgtaccg tcaggtttcc gcgcagattc acgccgtatt caggcgttat accgatttaa
    15781 tcgaaccctt gtcgctggac gaagcctatc ttgacgttac ccgtaatttc aaaaacatcc
    15841 cttacgccag tgaagttgcc aaagaaatcc gtgccgccat ttttgcggaa acaggtttga
    15901 ctgcatccgc aggcatcgcg ccgaacaaat ttctggcgaa aatcgcgtcg gactggcgca
    15961 agccgaacgg gcagtttgtg ttgccgccgc acaaagtcat ggcatttttg gaaaccctgc
    16021 ctttgggcaa aatccccggc gtgggcaagg taacgctgaa aaaaatgcag tcgctgggta
    16081 tgcggacggc gggcgacttg cgccgtttcg agcgcggcga actcttaaac catttcggac
    16141 gctacggata ccgcctctat gatttggtgc gcggtacgga cgaacgcccc gtcaaagccg
    16201 aacgcgaacg cctccaaatc tccacagaaa ttaccctgcc cgaagacctg ccgctcgagc
    16261 aggctgccgg acacctcccc catcttgccg aagacttgtg gcggcaaatc acgcgcaaaa
    16321 acgtcgaagc ccaaagcgta acgctcaagc tgaagaccta cgatttccgc atcatcacgc
    16381 gcacactgac ttattcctcc gtattgcccg actgcgcagc tctgctgcag gctgcgcaaa
    16441 tgttgatggc gcgcgtcccg ccgcagacgg aagacgcgtt ccgccttatc ggtatcggcg
    16501 tggggcatct tgtgccgaaa aaccagcagc aggatttgtg ggcgtaaacc gctttacgcg
    16561 cgccgtccaa aaaatatgcc gtctgaacgg ttttcagacg gcatggc
//
LOCUS       DAXHNE010000029        16512 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.029, whole genome shotgun sequence.
ACCESSION   DAXHNE010000029 DAXHNE010000000
VERSION     DAXHNE010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16512
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.029"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            38..643
                     /locus_tag="ACFM4Z_000741"
     CDS             38..643
                     /locus_tag="ACFM4Z_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HFC6020809.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            715..1107
                     /locus_tag="ACFM4Z_000742"
     CDS             715..1107
                     /locus_tag="ACFM4Z_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFC6020810.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(1136..1285)
                     /locus_tag="ACFM4Z_000743"
     CDS             complement(1136..1285)
                     /locus_tag="ACFM4Z_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020811.1"
                     /translation="MPSEARIAGSDGIFNIPISNRQGQHGFDTPKRVLPINRFRLRPI
                     GQNAV"
     gene            complement(1306..1752)
                     /locus_tag="ACFM4Z_000744"
     CDS             complement(1306..1752)
                     /locus_tag="ACFM4Z_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFC6020812.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTDAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTE
                     VEAAVAETGAAGMADMGKVMGLLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(1884..2096)
                     /gene="rpsU"
                     /locus_tag="ACFM4Z_000745"
     CDS             complement(1884..2096)
                     /gene="rpsU"
                     /locus_tag="ACFM4Z_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFC6020813.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(2385..4235)
                     /gene="ltgA"
                     /locus_tag="ACFM4Z_000746"
     CDS             complement(2385..4235)
                     /gene="ltgA"
                     /locus_tag="ACFM4Z_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase LtgA"
                     /protein_id="HFC6020814.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKS
                     LGARRQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAIKRALVLFRNSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVMGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMANAAYYAALFGAPHIPLKQRMGIVPAR"
     gene            4612..5349
                     /locus_tag="ACFM4Z_000747"
     CDS             4612..5349
                     /locus_tag="ACFM4Z_000747"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFC6020815.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALASKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFTVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHAQSDIGRELIRED"
     gene            5351..6037
                     /locus_tag="ACFM4Z_000748"
     CDS             5351..6037
                     /locus_tag="ACFM4Z_000748"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFC6020816.1"
                     /translation="MADLTFQQAVSNIIGMKGEIVRALGETFVMVGLSTTFAVIFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPVAIVCKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NTLARKLDKR"
     gene            6196..7059
                     /locus_tag="ACFM4Z_000749"
     CDS             6196..7059
                     /locus_tag="ACFM4Z_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC6020817.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAEKKE
                     IVFGTTVGDFGDMVKEHIQPELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(7121..7690)
                     /locus_tag="ACFM4Z_000750"
     CDS             complement(7121..7690)
                     /locus_tag="ACFM4Z_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFC6020818.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            7848..8708
                     /locus_tag="ACFM4Z_000751"
     CDS             7848..8708
                     /locus_tag="ACFM4Z_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFC6020819.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGRIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            8843..9181
                     /locus_tag="ACFM4Z_000752"
     CDS             8843..9181
                     /locus_tag="ACFM4Z_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020820.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRSNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGSC"
     gene            9549..9902
                     /locus_tag="ACFM4Z_000753"
     CDS             9549..9902
                     /locus_tag="ACFM4Z_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFC6020821.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            9892..10758
                     /gene="atpB"
                     /locus_tag="ACFM4Z_000754"
     CDS             9892..10758
                     /gene="atpB"
                     /locus_tag="ACFM4Z_000754"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFC6020822.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            10815..11051
                     /gene="atpE"
                     /locus_tag="ACFM4Z_000755"
     CDS             10815..11051
                     /gene="atpE"
                     /locus_tag="ACFM4Z_000755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFC6020823.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            11122..11592
                     /locus_tag="ACFM4Z_000756"
     CDS             11122..11592
                     /locus_tag="ACFM4Z_000756"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFC6020824.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            11597..12130
                     /locus_tag="ACFM4Z_000757"
     CDS             11597..12130
                     /locus_tag="ACFM4Z_000757"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFC6020825.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            12141..13688
                     /gene="atpA"
                     /locus_tag="ACFM4Z_000758"
     CDS             12141..13688
                     /gene="atpA"
                     /locus_tag="ACFM4Z_000758"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFC6020826.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            13713..14588
                     /gene="atpG"
                     /locus_tag="ACFM4Z_000759"
     CDS             13713..14588
                     /gene="atpG"
                     /locus_tag="ACFM4Z_000759"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFC6020827.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            14626..16023
                     /gene="atpD"
                     /locus_tag="ACFM4Z_000760"
     CDS             14626..16023
                     /gene="atpD"
                     /locus_tag="ACFM4Z_000760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFC6020828.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            16034..16456
                     /locus_tag="ACFM4Z_000761"
     CDS             16034..16456
                     /locus_tag="ACFM4Z_000761"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFC6020829.1"
                     /translation="MSIMQVEVVSSEQNIYSGEASFVVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
BASE COUNT         3837 a         4329 c         4377 g         3969 t
ORIGIN      
        1 gccgttttgc aggcatcaca caggagcgac aattattatg atgaccctct attccggcat
       61 tacctgcccc ttcagccacc gctgccgctt cgttttgtac gaaaaaggta tggattttga
      121 aatcaaagac gtcgatattt acaacaaacc cgaagacctc gccgtcatga atccgtataa
      181 ccaagttccc gtgctggtcg agcgcgattt ggtgctgcac gagtccaata tcatcaacga
      241 atacattgac gaacgcttcc cccatccgca gctgatgccc ggcgatcccg ttatgcgcgg
      301 tcggggccgg ctggtgctgt accgtatgga aaaagaattg ttcaaccacg tccaagtgtt
      361 agaaaacccc gccgccacca acaaggaaca ggcaaaagcg cgcgaagcca tcggcaacgg
      421 tctgaccatg cttgcccctt cgttcagcaa aagcaaatac atcctcggcg aagatttttc
      481 tatgattgat gtcgcccttg ctccgctgct gtggcggctc gaccactacg atgtcaaact
      541 gggcaaaagt gccgcgccgc tgctcaaata cgccgagcgc atcttccagc gcgaagcctt
      601 tatcgaagca ctgacacccg ccgaaaaagc catgcgcaaa taagtccgaa atgcttgcaa
      661 aacccaccgt tttgcaggca ttttcctatt ttggcgtaca acacggaacc cattatgccc
      721 acttccacca aaccctacat cctccgcgcc ctctgcgaat ggtgcagcga caacagcctc
      781 acaccgcaca tccttgtctg ggtcaacgaa cacacgcgcg tccccatgca gtacgtccgc
      841 gacaacgaaa ttatgctcaa catcggcgcg accgccacgc aaaaccttca aatcgacaac
      901 gattggatca gcttttccgc ccgcttcggc ggacaggcgc acgatatatg gatacctgtc
      961 ggacacgtcc tcagcctttt cgcacgggag accggagaag gtatggggtt tgagttggaa
     1021 gcgtaccgcc ccgatacgcc gcctgaaaac acctctgccg aaaccgcgcc ccgacccgcc
     1081 aaaaaaggct tgaaattggt caaataaatc tatgccgtct gaacggaatc gtgtttcaga
     1141 cggcattttg tccgatgggg cgcaaacgga atctgtttat cggcaaaacc cgtttcggcg
     1201 tatcaaaacc gtgttgcccc tgccgattcg atattgggat attgaaaatg ccgtctgaac
     1261 ctgcgatacg ggcttcagac ggcattttgt ccgatattcg ggcaatcagg cggtcagcac
     1321 ggctttcagg attttgttga cttcgcccat gtcggcttta cctgcgaggc gggttttcag
     1381 cagccccatg actttaccca tatccgccat acctgccgcg ccggtttcgg caacggcagc
     1441 ttcgacctcg gtacggattt cgccggcgga aagcatttgg ggaaggtagc ggtgcagtac
     1501 ctcgatttcg gcgttttctt tgtctgccaa gtcctgccgc cccgcgtcag tatagatttt
     1561 cgcgctgtct ttgcgctgtt tgaccatttt ggtcaggatg gcggtgattt tggcatcgtc
     1621 ggcttcggtg cgttcgtcca cttcaaactg tttgacggcg gcgttgataa ggcggatggt
     1681 gccgagggaa acttggtctt tggcgcgcat agccgttttc atgtcttcgg taaggcggat
     1741 tttcaggctc ataatgttct cgctgggatg tcggatggaa acggcgggtt tcgatgccgt
     1801 ctgaaaagca aaacacaccg cagagcaggt cttgcggtgt gtcctcatat cacagggctg
     1861 acctgtaatt tgtacttgaa tgtttagtac attttgggcg gcagttgttg gctgcgcagg
     1921 cgtttttgca ggcgttttac ggctgccgct tttttgcgtt tgcgttcggt agtcggtttt
     1981 tcgtaggctt cgcgggcgcg cagctcggtc agcagaccgg ttttttctac ggcgcgtttg
     2041 aaacggcgca tagcgacttc aaatggttca ttctctttta cgcggattgc aggcatttta
     2101 tttcctttaa taaattcggt tgtttcatct gcccatcata tcggtggaaa gggtagggca
     2161 gacggtgttg aaggtttacc gtatcgctgt gccttgcggc ggcgggatac gcgccgtgac
     2221 ggaaacatca cctcctaacg ggtcggcttg tgccgtgtca agctgcttga aaaagcagga
     2281 aaaacaattt tcggattgtc ttatatttat gattcgccgt caatgccgtt cgggataaaa
     2341 atgccgtctg aaagaccggg cgggtttcag acggcatcgg tacgtcagcg ggcggggaca
     2401 atgcccatac gctgtttgag cgggatgtgc ggcgcgccga agagggcggc gtagtaggcg
     2461 gcattggcca tcactttttt gacatagtcg cgcgtttcgg aaaacgggat ggtttcggca
     2521 tataccgcgc cttcgagggg cgtgtccgcc tgccatcggc gcgccctgcc gggaccggcg
     2581 ttatagcctg cggtggcgag gacttcgttg ttttgcaggc ggcgtttggt gtccgccata
     2641 taccacgtcc ccatacggat attgccgtcg gcggtgtaaa gttgtgcggc atccataccg
     2701 attttgccgg cgatttcgcg cgcggtggca ggcataacct gcatcagccc ctgcgcgcct
     2761 acgcgggatt gcgcgcccat aacgaagcgg ctttcctgac gaatcagccc gtaaacccac
     2821 gccggatcga cattaacatt ttgcgcgtgg cggattaccg tgtctttaaa cggcgaaatg
     2881 tagcgcaagg tgtagttgag tttgcggtcg gtgcgttccg cgctgttgac cgccatatcg
     2941 taaaaaccgt ggtcgaacgc ggtttgcgcg gcggtcagca gcttgtcttc atcgaagccg
     3001 cgtgtggcga aacgccattc cgcctgagcc tgacggcgca ttttcgcatc gccggcggtt
     3061 cggctgtttc ggaacagcac cagcgcgcgc ttaatcgcgc cgtcttccgc catacggagg
     3121 acgctgtttt tgccggcatc gggcacattg ttgcgcgtat cgattttgcg ccccaactct
     3181 tcccccgcca gcaccgcata aaaattcctg cccgtcgctg ccgcctgttt gtaaagtttt
     3241 tccgcctctt gcgtgttgcc cgttgcggcg cgactgcgtg ccagccagta gagccaggtc
     3301 gggctttttt gcagtttttc gggcatatgc gagattacgg aagccagctc gtcccaacgt
     3361 cgggcgcgca aggcggcgcg ggcgtaccac tcgatttggt cgtcggtcag ttggcggcgg
     3421 tcggcaactt tgccgtaata gtccaaggcg gcaggcacat tgaggttttg cgactgataa
     3481 tgccccaata cgccccacgc gaaactgcgt tgttcgaggc ttaaaccgct ttccatttcg
     3541 gacagcaggg cggcggcatt cggcgatttg cgtgcttctt tgccgatgac gttcaacagg
     3601 gcgtattcgc gcgaaccttg tgtaccgccg tcaaacgggc tgcccaatgc ggcggcaagg
     3661 ttgcgtgcgt ctgtggtttg gcggccggcc agcagtccgc gcacgcgcct ccaggcgtcg
     3721 ttgccgtcca acaagccgga tgcggctgcc tgttccaaca gtttggtgca gcccgaaggc
     3781 agtttgcccg tatttttaac cagttcagcg gcacgcgtat agtcgttgcg gctcgaatcg
     3841 gcgtagcatt cgacttcttg ggcgcgccct gccggttcga gtttggcgta ttcctgtgca
     3901 aacagcgtcc actgtctgcg tgcgcccaaa gacttcagcc actcgttgcg gacattttcc
     3961 gccatcgcgc tgtcgccggc gttttccaaa taggcggcga cggcggcatc gtttttctgt
     4021 ttcactgcat ccagtgcgga cgggtagccg ccgtaatctg ccagcgtttt tccttcgggt
     4081 tcggcagggc gggtgggaac gcttgccgaa aggtcggcag tttctatatt gtctgccggg
     4141 gtcttgccgg ctggcagtgt gtttgtcgaa gaacacgcgg caagcactag ggccgccagc
     4201 agcggcaggg aatgcttcat agagggtagg tacattggat ttccttaaga atcggaaccc
     4261 tgaacggtca gggttggaaa agacaaaatg ccgtctgaac aggcgtttgc atgaattata
     4321 tgccgaaact gcaccgcctt tggaatgttt ctgacataat ttatattttt caatcagttg
     4381 ccgttttggt gagaaccgct gcctttgccc gtttcagacg gcattgtccg aaatggttgc
     4441 ccgcttcctg ccttattgac aaaaaaacgc tttcccgata atatcctacg aaaattaacc
     4501 tgccgatttg acacagcttg cgggcataac agctaaagcg ttccgacaat ttcagcttta
     4561 tcttccgcgc ccgttgtgtc cgacatcggg ctttgttgta tgggaaagac aatgattatt
     4621 ttggacaagg tttccaagca ttaccaaacg cgcgacaaga cccgttttgc cgccgtcgag
     4681 ccgaccagcc tcgaaatccg cgacggcgaa atcttcgggc tgatgggtta ttcgggtgca
     4741 ggcaaatcca ccctgttgcg cctgattaac ctgttggaac gtcccgacag cggcaaggtc
     4801 aacgtctgcg gacaagagct gaccgcgctc gatgccgccg cattgcgtca ggctcggcag
     4861 aatatcggca tggtgtttca gcagttcaac ctgttgaaca accgcaccgt cgccggcaac
     4921 gtcgccttcc cattggaaat cgccggatgg ccgtctgaaa aaatcaaagc gcgcgttgcc
     4981 gaatgtctcg aaatcgtcgg actgaccgaa cgcgccggcc actatcccgc ccagctttcc
     5041 ggcgggcaga aacaacgtgt cggcatcgcc cgcgcgctcg catccaagcc gcaagtcatc
     5101 ctcgccgacg aacccacttc cgccctcgat cccgccacca cgcgcagcgt cttggaatgt
     5161 ttggaagaca tcaacaaacg cttcaccgta accatcgtca tcgtaaccca cgaaatgagc
     5221 gtcatcagac gactgtgcga ccgcgccgcc ctcttggaca aaggcaaagt cgtcgaaatc
     5281 gtcgaagtac gcggcaacca aatccacgcc caatccgaca tcgggcgcga actgattcgg
     5341 gaggactgat atggcagact taacattcca acaagccgtt tccaacatca tcggtatgaa
     5401 aggcgaaatc gtccgcgcct tgggcgaaac ctttgtgatg gtcgggctgt ccaccacgtt
     5461 tgccgtcatt ttcggcacgc tcttgggcgt attgctcttc gtaacttcca gccgccagct
     5521 gcattacaac aaaccggtaa atttcctgct cgacaactta gtcaacctga tgcgcgcctt
     5581 ccccttcgtc attttgatga ttgcgatgat acccgccaca cgcgccatcg tcggcagcac
     5641 catcggcccg gttgccgcct cgctggtgtt gagcgtgtcg ggattgtttt attttgcccg
     5701 actggtggaa caaaacctgc gcgaagtccc caaaggcgtg attgaagccg ccgccgcgat
     5761 gggtgcgccg ccggttgcca tcgtctgcaa agtcctcttg aacgaagcgc gcgcgggcat
     5821 ggtttccagc attaccgtgc ttgccatcgg gcttttgtca tacagcgcgg cggcagggat
     5881 gataggcggc ggcggcttgg gcgacctcgc catccgctac ggctactacc gctaccaaac
     5941 cgaagtcatc gtcttcatcg tcgccctcct cgtgctgctg gtcatcctga ttcaaagcac
     6001 cggcaacacg ttggcgcgga aactcgacaa acgttaaacc cgaatgccgt ctgaacgcca
     6061 aaacccccac cgctatccga aaaatgctat aaaattcccc tgttcgcggc aaatgccgtc
     6121 tgaacgccga atccggacgg caggactccc tgcccgtcat ttttgtttga aaccgccaca
     6181 acatcaggag aaaatatgaa aaccttcttc aaaacccttt ccgccgccgc actcgcgctc
     6241 atcctcgccg cctgcggcgg tcaaaaagac agcgcgcccg ccgcatccgc ttctgccgcc
     6301 gccgacaacg gcgcggagaa aaaagaaatc gtcttcggca cgaccgtcgg cgacttcggc
     6361 gatatggtca aagaacacat ccaacccgag ctggagaaaa aaggctacac cgtcaaactg
     6421 gtcgagttta ccgactatgt acgcccgaat ctggcattgg ctgagggcga gttggacatc
     6481 aacgtcttcc aacacaaacc ctatcttgac gacttcaaaa aagaacacaa tctggacatc
     6541 accgaagtct tccaagtgcc gaccgcgcct ttgggactgt acccgggcaa gctgaaatcg
     6601 ctggaagaag tcaaagacgg cagcaccgta tccgcgccca acgacccgtc caacttcgcc
     6661 cgcgtcttgg tgatgctcga cgaactgggt tggatcaaac tcaaagacgg catcaatccg
     6721 ctgaccgcat ccaaagcgga catcgccgag aacctgaaaa acatcaaaat cgtcgagctt
     6781 gaagccgcgc aactgccgcg tagccgcgcc gacgtggatt ttgccgtcgt caacggcaac
     6841 tacgccataa gcagcggcat gaagctgacc gaagccctgt tccaagaacc gagctttgcc
     6901 tatgtcaact ggtctgccgt caaaaccgcc gacaaagaca gccaatggct taaagacgta
     6961 accgaggcct ataactccga cgcgttcaaa gcctacgcgc acaaacgctt cgagggctac
     7021 aaatcccctg ccgcatggaa tgaaggcgca gccaaataag gcagtcgtat aaaatgatgc
     7081 cgtctgaacc gtatccgtgt tcagacggca tttttgtcct ttaatccgcc attccctgcc
     7141 attccgccga atccggcgta tcgattccga acagcgacaa agcgtgtgcg acactgtgcg
     7201 ccactatgtc gtccgccgtc tgcggtttgc ggtacatcgc aggaacaggg ggaaacacca
     7261 cgccgcccat ttccgttacc cgcttcatat tgtccaaatg ggcaaggttc agcggcgttt
     7321 cgcgcaccat cagcaccagc cgccgccttt ccttcaaaac cacatccgcc gcacgcgtca
     7381 agaggttgtc gccgaagccg tgcgcgacag aggcaagcgt ccgcatcgaa cagggggcga
     7441 ccagcatccc gtccgtttta aacgtaccgc tggcaatgca cgccccgata ttgccgatcg
     7501 gatgcacgaa gtccgccaag gcatatacct catctctcgc ataagccgtt tccgaagcgc
     7561 gcgccatctc cgcacctttc gataccacaa ggtgcgtttc gatatcttgc gcgcgtaaaa
     7621 gttccaaagc cttcacgccg tattggaaac cgctcgcccc gctgatgccg attatcaaac
     7681 gccgtaccat catccgcctt tcccataaaa ccgcctgcaa cggcaaaccg gctattatag
     7741 tgaaaaaaca gaaatccgat aaacacggat acaaattgtc ggcaacaccc aatatccgat
     7801 aaaatacccg atttaacatc ctatctgaat aggcacggga gggcggtatg gcaaaagtaa
     7861 aaggcggatt ggggcgcggt ttggattcgc tgctcgccaa cggcgcagac aacagcagcg
     7921 gcgaccgatt gaccacggtt gcggttaaag atatccggcc cggccgctat caggcgcgtg
     7981 ttcaaatcga tgacgaagcc ttgcaggagc tggcagattc gattaaggcg caaggcgtga
     8041 tacagcccgt catcgtgcgc gaacacggac tgtcccgata cgaactgatt gcgggggagc
     8101 ggcgttggcg tgccgcacag attgccggcc tgaccgaaat ccccgccgtt atcaaaacca
     8161 tcagcgacga aaccgcgttg gcaatgggtt tgatcgaaaa cctccagcgc gaaaacctca
     8221 accccatcga agaagcacaa ggcttgaaac gccttgccga cgagttcgga ctgacccacg
     8281 aaaccatcgc ccaagccgtc ggtaaaagcc gaagcgcgat ttccaacagc ctgcgccttt
     8341 taagcctgcc cgaacccgtg caggaaatgc tttaccaacg ccgcctcgaa atggggcacg
     8401 cccgcgcact gctgaccctg cccgtcgtcg aacagctcga gttggcgcaa aaggccgtca
     8461 aaaacggctg gtcggtgcgc gaagtcgagc gccgcagcca agccgccctt caaaacaaac
     8521 gtcccgagcc caaaaaaact gccgccgccg acatcggccg cctgaatgat ttgctgactg
     8581 aaaaactggg tgtcaacgcc gaaatcaaaa ccgccaacca taaaaaaggc agaatcatcc
     8641 tgcatttcga cacgcccgaa accttcgacc atatcttgaa gcagttgggc atagattacc
     8701 gaccttaatt ttgcgggata taccgtctga aatatagaga atagctttcc agattgtaag
     8761 tgggaaatat aattctattg acatttttct gcttcacgta agaatcgttt tcctgttttc
     8821 atttttaatt ttcgaggaaa ttatgaacac acgcatcatc gtttcggctg cgttcgttgc
     8881 gttggcatta gcaggttgcg gctcaatcaa taatgtaacc gtttccgacc agaaacttca
     8941 ggaacgtgcc gcgtttgcct tgggcgtcag cccaaatgcc gtaaaaatca gcaaccgcag
     9001 caatgaaggc atacgcatca actttaccgc aactgtgggt aagcgcgtga gccaatgcta
     9061 tgttaccagt gtaatcagca caatcggcgt taccacttcc gatgcaattt gtttgggagg
     9121 cggaacgcac aaaggcaaaa gtcaatgcaa tgctttgctt aaagcggcag gcagttgcta
     9181 atcctttatt cggaaaaggt cgtctgaaaa tattttcaga cgatctttta tttattgagc
     9241 aaattcccca aactgccgac aatccgaccg ataaactgct acaatttcgc gttgattttt
     9301 tattatttcc acgtaaagca aacaaatcac gtaagaacat taaaaatgta aagaaattgc
     9361 ggacaaactc cttaatatcc tgtaattata tgtaatgtgt gttagaatga cacttgtcgc
     9421 cgttattcag gcggcgagcg cggcggattg ggatacgttg tttcacaaag attaactgtc
     9481 gcttgacgta ataaggcttc taaattatat tagccgcgcc ccaaatccga ggcggaaaca
     9541 ttccctttat gaagcagatt atcatcctac aatccgccgt attgtccata tgcgccgcag
     9601 ttgcctttgc cgtatggggt tttgccggat tcctttcggc ggtcggtggc ggtttgtcct
     9661 acctgcttcc cacgtttgtt gcagttttac ttttaaaact tttcagggga aaccccttcc
     9721 tgcaaagcag gatgttcgtc ttcggagaga ttttaaaagt agtgctgtcg ctgttgtcca
     9781 tgctcgccgt atttgcaata tggcatcaat cgctggtgtt cgccccgttt ctgatggggc
     9841 tgctcggtgt cagccatttg gtttttttag tattgttgag agtgaaagat tatggcaggt
     9901 gaaaccatta ccgctgccga ctacatcaag caccacttgc agagcttgac cagtttgtcg
     9961 gatgttactc agggtcaggg actgaaaaac attgctgatt tttcgtttat taaccttgat
    10021 gccgtctttt ttgccgtcct gttgggcgta atcggcagct tcctgttgtg gcgcggtgca
    10081 aaaaaagcga cggcaggcgt tcccggacgt ttccaggccg ccgtggaaat cttgtttgag
    10141 tttgtggacg atatgtgtaa gagcatcatt cacaacgaaa agtctagaaa agccgtcgcg
    10201 ccgttgggtc tgacgctgtt tgtctggatt ttcctgatga acgcgatgga tatgctgccg
    10261 gtcgatttgc tgccgatggt atggcagggc attaccggca accatcacgc cctgttgcgc
    10321 gtcgtaccga ctgccgattt gaacaccact ttggcactcg ctgtcggcgt gttgctgatt
    10381 tgtatctatt acaacatcaa aatcaaagga ttgggcggat ggtttcacga gttgttcagc
    10441 gcgccgttcg gtgcaaaact cgcacctgcg aacttcctgc tgaatttggt cgagtttctt
    10501 tctaaaaccg tatcccacgg tatgcggttg ttcggcaata tgtatgcagg cgagttggta
    10561 ttcttgttga tagccttgct tggcggtgct tgggcggctt ccggcagcgt cgaagtcatg
    10621 gatccgattc tgtttgtatt ccacattatt gccggtttgg catgggcgat tttccatatt
    10681 ttggtgatta ccctgcaggc gtttattttc atggcgttgg cgttcgtcta tatcggacag
    10741 gcacatgatg cgcactgatt ttcctaagta gtttgttttt gtagtttaac ctttgtttct
    10801 ttaaggagtt taaaatgggt ttgattgcta tcgcatgtgg tttgatcgtt gcattgggtg
    10861 cattgggtgc ctctatcggt atcgcaatgg tcggttctaa atatttggag tcttctgccc
    10921 gtcagcccga actgatcggt ccgctgcaaa ccaaactgtt cctgattgcc ggtttgattg
    10981 atgccgcatt cttgattggt gtcgccattg cactgctgtt cgccttcgtt aacccgtttg
    11041 caggtgcata atcaggacgg attcgtccgt tccgtacagg cgcagcctgt ttgattaacc
    11101 ccagtacgaa ggttaagtaa cgtgaatatc aatgcaacat tattcgctca aatcatcgtc
    11161 tttttcggtt tggtatggtt taccatgaaa ttcgtgtggc cgccgattgc aaaagctttg
    11221 gatgagcgtg ccgcaaaagt cgccgaaggc ttggctgccg ccgagcgtgg taaaagcgat
    11281 ttcgagcagg ctgaaaaaaa ggttgcagaa ctcttggcag aagggcgtaa tcaggtttcc
    11341 gaaatggttg ccaacgccga aaaacgtgcc gccaaaattg tcgaagaagc caaagaacag
    11401 gcttcttccg aggcggcgcg cattgcagct caggcaaagg ccgatgtgga gcaggaattg
    11461 ttccgcgcac gcgaatccct gcgcgagcag gttgccgtgt tggctgtcaa aggtgccgaa
    11521 tctattttgc gcagcgaagt cgatgcttcc aaacacgcaa aactgctcga taccctgaaa
    11581 caggagctgt aatcttatgg cagagttcgc aacgattgcc agaccttatg caaaggcatt
    11641 gttcggtctg gctcaggaaa aaaaccaaat tgagtcttgg ttgggcggac tggaaaaact
    11701 tgcggcggtt gttcaggaag ggaaggtggc ttcattgatt gaccgtcctg aaacgaatgc
    11761 ttcagaaaaa gcagatatcc tcatcgattt ggtcggtttg aaagacaagg agttgaaaaa
    11821 ctttgttatc gtcttggccg ggcagaaacg tttgtcgata ttgccggaag tatatgctca
    11881 atatcaagac ttgaccttat cattcaacca tatcaaatct gccgtcattt acagtgccta
    11941 tccgttgacc gacaaacagg tcggcgagtt ggcgcaaatg ctgaataagc gtttcgacag
    12001 cgagctgaaa atctctgtcg aaatcgaacc ggagctgatt ggcggcataa aagttgaagt
    12061 gggtgatcag gttttggatt tgtctgtaca aggcaaattg agtgccttgt acacgactat
    12121 gacgaattag gagagttttc atgcagctta atcctgctga aattagcgat ttgattaaag
    12181 ccaagatcga aaatctgtct gtaaatgccg aagtgcgtac tcgtggtacg gtgatttcgg
    12241 taactgacgg tatcgttcgc atccatggtt tgtcagatgc aatgcaaggt gagatgctcg
    12301 aattcccggg taacactttc ggcttggcga tgaacctgga gcgcgactcc gtcggcgccg
    12361 tagtgttggg cgagtacgaa catattaaag aaggcgacac ggttacctgt accggtcgta
    12421 tcttggaagt gccggtcgga cgcgaactgg tcggacgcgt cgttgatgca ttgggtcggc
    12481 ctatcgacgg caaaggcccg attaatacaa ctttgaccgc ccctatcgaa aaaatcgcac
    12541 cgggcgtgat tgcgcgcaaa tcggttgacc agccgatgca aaccggtctg aaggcgattg
    12601 actctatggt tcctgtcggt cgcggtcagc gtgagttgat tattggcgac cgtcaaaccg
    12661 gtaaaacggc cgtagcattg gatgccatcg tcaaccaaaa aggtacgggt gttatctgta
    12721 tctatgtcgc tatcggtcaa aaagcatctt ctattgccaa cgtggtccgc aaattggaag
    12781 agcatggcgc gatggaacac acgattgtgg ttgctgcaac tgcatctgaa gcggcggcat
    12841 tgcaatatat cgcaccttac tccggttgta cgatgggtga attcttccgc gatcgtggcg
    12901 aagatgcctt gattgtttat gacgatttgt ccaaacaggc tgtggcttac cgtcaaattt
    12961 ccctgctttt gcgccgtccg cccggccgcg aagcctatcc tggcgatgtg ttctacctgc
    13021 actcccgtct gttggaacgt gcggcacgtg tcaatgaaca cgaagtggag aaattgacca
    13081 acggcgaagt aaaaggcaaa accggttctc tgaccgcgtt gccgattatc gaaacccaag
    13141 cgggcgacgt atctgctttc gttccgacta acgtcatttc gattaccgac ggtcagattt
    13201 tcttggaaac cgacctcttc aacgccggta tccgtcctgc aatcaatgcc ggtatttccg
    13261 tatcccgcgt aggcggtgct gcacaaacca aagtgattaa aaagctgggt ggcggtatcc
    13321 gtttggcgtt ggcacaatat cgtgaattgg cggcgttctc gcaatttgca tccgatttgg
    13381 atgaagctac gcgcaaacaa ttggagcatg gcgaagtcgt aaccgaactg atgaaacaga
    13441 aacagttcag tacgttgaat acggctgaaa tggctttgac cctttgggca atcaacaacg
    13501 gttcgtattc tgatgttccg gttgctaaag ctctggcttt tgaatctgaa tttttgagct
    13561 ttgtccgtac ccaacatccg gaagttttgg aagccgtcaa tgcttcaggt gcaatgtccg
    13621 acgagagcga gaaaacgctt gaagcagcca tgaaatcctt caaatcttct tacgcctatc
    13681 aggcataagg cttgaagtga aaggagtctg aaatggcagt aggaaaagag attctcacca
    13741 aaatccgcag tgttcagaat acccaaaaga tcactaaagc gatgcagatg gtgtcaacct
    13801 ctaaaatgcg gaagactcag gaacggatgc gtttggcgcg tccgtatgcc gaaaaagtgc
    13861 gtatggtgat gagccatctt gcgcaaacca ataccgatca tggtattccg ttactggaat
    13921 ctcatcggga aatcagacgt gtcggtttta ttttgattac gtctgataag ggtttgtgtg
    13981 gcggtttgaa cgccaacgtg ctgaaaaagt ttttggcaca agttcaagag tatcggaatc
    14041 aaggtattga agaggaaatc gtatgccttg gcagtaaagg tctgatggcg tgtcagagca
    14101 ttggtctgaa tgtggttgcc agtgccgtaa atttgggcga taccccaaaa atggaaatgc
    14161 tgctcggacc tttgacagaa ctcttccaac ggtatgagaa acatgaaatt gacagaatcc
    14221 atctggtgta ttcgggtttt gtcaatacca tgcgtcaaga accgagaatg gaagtattgc
    14281 tgcctatcgg tgagaacgtg attggcgatt cagctcccaa atcaccgttc agctgggaat
    14341 accgctacga accgactgca cttgcagtgt tggaatatct ggttcgccgc tatttagagt
    14401 ctgtggttta tcaggcgttg agcgacaata tggcatccga acaggcagcc cgcatggttg
    14461 ccatgaaagc tgcaacagac aatgcaggca atgccatcaa agagttgcgt ttggtatata
    14521 acaaatcgcg tcaagctgcg attaccacgg aattgtcaga aattgtagca ggtgcggcgg
    14581 ctgtctgatg ccgtctgaat cctgtacgga aattaggata cgataatgag ccaaggcaaa
    14641 atcgtacaaa ttatcggtgc ggttgttgac gtggaatttc cacgcgacat gattccgcgc
    14701 gtttacgacg ctttgaaatt agacgaaaac ggtctgactt tggaagtcca acagcttttg
    14761 ggcgacggcg tagtccgtac cattgcgatg ggcagctcgg acggtttgaa acgcggcatg
    14821 actgtgagca ataccggtgc gcccattact gtgccggtag gtaaaggtac gttgggacgc
    14881 attgtcgatg tattgggaac gcctgttgac gaggcaggtc caatcgatac cgacaagagc
    14941 cgtgccatcc accaagccgc tcctaagttt gacgaactgt cttccacaac cgaattgctc
    15001 gaaacgggca ttaaagtgat tgacttgctg tgtccgtttg ccaaaggcgg taaagtaggt
    15061 ctgttcggcg gtgccggtgt gggtaaaacc gtgaacatga tggaattgat caacaacatc
    15121 gccaaagcgc acagcggctt gtccgtgttc gcaggcgtgg gtgagcgtac ccgcgaaggt
    15181 aacgacttct accacgagat gaaagattcc aacgtattgg ataaagtggc aatggtttac
    15241 ggtcagatga acgaacctcc gggcaaccgt ttgcgcgtcg cattgaccgg tttgaccatg
    15301 gcggaatact tccgtgacga aaaagacgaa aacggcaaag gccgcgacgt attgttcttc
    15361 gtggacaaca tctaccgtta cactctggct ggtaccgaag tatccgcatt gttgggccgt
    15421 atgccgtctg cagtgggcta ccaaccgaca ttggcagaag aaatgggtcg tttgcaggag
    15481 cgtattacct ctacccaaac cggttctatt acttctatcc aagccgtata tgtacctgcg
    15541 gacgacttga ccgacccgtc tccggcaaca acgttcgccc acttggacgc aaccgtcgta
    15601 ttgagccgcg atattgcctc tttgggtatt tacccggcag tcgatccgct tgattccact
    15661 tcgcgccaac tggatccgat ggtattgggt caagagcact acgacgtggc gcgcggcgta
    15721 cagtccaccc tgcagaaata caaagaattg cgcgacatca tcgccatttt gggtatggac
    15781 gaattgtctg atgaagacaa actgaccgta atgcgtgcgc gtaaaatcca acgcttcctg
    15841 tcccaaccgt tccacgttgc cgaagtgttt acaggttctc cgggcaaata tgtcgccctg
    15901 cgcgatacca ttgccggctt caaagccatc ttgaacggcg aatacgacca tctgcccgaa
    15961 caggcattct acatggtcgg cagcatcgaa gaagcggttg agaaagcgaa aaccttaaac
    16021 tgaggaggtc ggcatgagca tcatgcaagt tgaggtggtc agtagcgagc aaaacatcta
    16081 ttcgggcgaa gccagctttg tagtggttcc gaccgttcaa ggtgagcttg gtatttaccc
    16141 gcgacacgag ccgattatga gtttggtgcg gccgggggct ttgcgtttga ccgttccggg
    16201 cgaggataaa gaggttttgg ttgctgtttc cggcggtgtt ttggaagtac agcctgataa
    16261 agtaactgtc ttggcggatg ttgccgtccg cagtgcggag atggatcagg cacgtgcgga
    16321 agaggcgaaa aaagccgcag aagcgggcat ttcccaagct aaagacgata aggctttggc
    16381 ggaggcgcat aaagcattgg ctgctgcgat tgcacagctc aaaactttgg actatatccg
    16441 ttcgcacaag aaataaactt gtttggtttg gaaaagcacg accgtttggt cgtgcttttt
    16501 atttttccaa gt
//
LOCUS       DAXHNE010000030        16507 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.030, whole genome shotgun sequence.
ACCESSION   DAXHNE010000030 DAXHNE010000000
VERSION     DAXHNE010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16507
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.030"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            209..1273
                     /locus_tag="ACFM4Z_000762"
     CDS             209..1273
                     /locus_tag="ACFM4Z_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HFC6020830.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1414..1737
                     /locus_tag="ACFM4Z_000763"
     CDS             1414..1737
                     /locus_tag="ACFM4Z_000763"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HFC6020831.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1809..2132
                     /locus_tag="ACFM4Z_000764"
     CDS             1809..2132
                     /locus_tag="ACFM4Z_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFC6020832.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2179..2382
                     /gene="tatA"
                     /locus_tag="ACFM4Z_000765"
     CDS             2179..2382
                     /gene="tatA"
                     /locus_tag="ACFM4Z_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFC6020833.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2386..3072
                     /gene="tatB"
                     /locus_tag="ACFM4Z_000766"
     CDS             2386..3072
                     /gene="tatB"
                     /locus_tag="ACFM4Z_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFC6020834.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKEIGTDMEGNLHDISNGLKPWEKLP
                     EQRTPADFGVDENGNPLPDAANTLSDGISDVMPSERSYASAETLGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRGLRPKHR
                     AKPKLRVRKS"
     gene            3087..3857
                     /gene="tatC"
                     /locus_tag="ACFM4Z_000767"
     CDS             3087..3857
                     /gene="tatC"
                     /locus_tag="ACFM4Z_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HFC6020835.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGMMPFAQQ
                     LYTFIANPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLAGVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTEQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPATT"
     gene            3910..4515
                     /locus_tag="ACFM4Z_000768"
     CDS             3910..4515
                     /locus_tag="ACFM4Z_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HFC6020836.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYQVVKLPANIDETVRQNEDPARY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSAGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIRYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGNFSGIMGLPVYETVSMLQDLGYRTPPFIRA"
     gene            4531..4884
                     /locus_tag="ACFM4Z_000769"
     CDS             4531..4884
                     /locus_tag="ACFM4Z_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HFC6020837.1"
                     /translation="MNRQTTYLLASFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4874..6688
                     /locus_tag="ACFM4Z_000770"
     CDS             4874..6688
                     /locus_tag="ACFM4Z_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HFC6020838.1"
                     /translation="MPAETTVSGAHPTAKLPIYILPCFLWIGIVPFTFALRLQPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPPISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACRSLVAHYGQERIVTLFAWSLLIGSLLQSCIVVIQFTGWEDTPL
                     LQNIIVYSGQGVIGHIGQRNNLGHYLMWGILAAAYLNGQRKIPAALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIHDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6878..7555
                     /gene="misR"
                     /locus_tag="ACFM4Z_000771"
     CDS             6878..7555
                     /gene="misR"
                     /locus_tag="ACFM4Z_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator MisR"
                     /protein_id="HFC6020839.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7568..8974
                     /gene="misS"
                     /locus_tag="ACFM4Z_000772"
     CDS             7568..8974
                     /gene="misS"
                     /locus_tag="ACFM4Z_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     MisS"
                     /protein_id="HFC6020840.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIVVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9092..11518
                     /locus_tag="ACFM4Z_000773"
     CDS             9092..11518
                     /locus_tag="ACFM4Z_000773"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6020841.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EENQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQSAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALEAADPALIAEAL
                     HLPPYRHTTHNAVQFQFGSHSLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTIIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     YPKTLSLRNNTTVTITPILPEDAEAKQQFVRSLGPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLRTDHKIT"
     gene            11563..11808
                     /gene="rpsP"
                     /locus_tag="ACFM4Z_000774"
     CDS             11563..11808
                     /gene="rpsP"
                     /locus_tag="ACFM4Z_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFC6020842.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11824..12333
                     /gene="rimM"
                     /locus_tag="ACFM4Z_000775"
     CDS             11824..12333
                     /gene="rimM"
                     /locus_tag="ACFM4Z_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFC6020843.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTVSVTLEAGKVVNSELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            12333..13082
                     /gene="trmD"
                     /locus_tag="ACFM4Z_000776"
     CDS             12333..13082
                     /gene="trmD"
                     /locus_tag="ACFM4Z_000776"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFC6020844.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQAAKVIYLSPQGKPLTHQKAVEL
                     AELPHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRSLIPKESRILNKILQEQREIQS"
     gene            13098..13463
                     /gene="rplS"
                     /locus_tag="ACFM4Z_000777"
     CDS             13098..13463
                     /gene="rplS"
                     /locus_tag="ACFM4Z_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFC6020845.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13785..14510
                     /locus_tag="ACFM4Z_000778"
     CDS             13785..14510
                     /locus_tag="ACFM4Z_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFC6020846.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFR
                     GINAVQKQRVHESLERVGMRQFAGRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPEWCAS"
     gene            14546..15421
                     /locus_tag="ACFM4Z_000779"
     CDS             14546..15421
                     /locus_tag="ACFM4Z_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFC6020847.1"
                     /translation="MNPYDLLITPFAEFDFMRYALVSVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSSLTLITLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15498..16424
                     /locus_tag="ACFM4Z_000780"
     CDS             15498..16424
                     /locus_tag="ACFM4Z_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFC6020848.1"
                     /translation="MKHPKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHDHDHEGHHHNHGEYDPHVWNDPVLMSTYAQNVAEALI
                     KADPEGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHI
                     EFIAPQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSG
                     KLYSDALGNAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         4304 a         5301 c         3627 g         3275 t
ORIGIN      
        1 ccgtctgaag ccgattcggt tttcagacga catcattatt tgacctgacg caaacagaaa
       61 gccttccgcg tgcttaaaca catctcttgt agtaaaatat ctaaacagat tcaactggaa
      121 acggtcaggc ttcttcacgc cattcccatt tcaaccgccg cctgccagat tcagacggta
      181 ttcctacttg aaaacaagga gcgttacaat gaaagcagca cgtttttacg ataaaggcga
      241 catccgcatc gaagacatcc ccgaaccgac cgtcgccccc ggcactgtcg gcatcaatgt
      301 cgcctggtgc ggcatctgcg gtactgacct gcacgaattc atggaaggcc cgattttcat
      361 tccgccttgc ggtcatccgc acccgatttc cggcgagtcc gcacccgtca cgatgggaca
      421 cgagttctcc ggcgtggtct atgccgtcgg cgaaggcgtg gacgacatca aagtcggcca
      481 acacgtcgtg gtcgaaccct acatcatccg cgatgacgta ccgaccggag aaggcagcaa
      541 ctaccacctc tccaaagata tgaactttat cggcttgggc ggctgcggcg gcggtctgtc
      601 cgaaaaaatc gccgtcaaac gtcgttgggt gcatccgatt tccgacaaaa tcccgttgga
      661 tcaagccgct ttgatcgaac cgctgtctgt cggacaccac gcctatgtac gcagcggcgc
      721 gaaagcaggc gacgtcgcat tggtcggcgg cgcaggtccg atcggtttgc tgttggctgc
      781 cgtgttgaaa gccaaaggca tcaaagtcat catcaccgag ttgagtaaag cacgcaaaga
      841 caaagcgcgc gaatccggcg ttgccgacta catcctcgac ccgtccgaag tcgatgttgt
      901 cgaagaggtg aaaaaactga ccaacggcga aggcgtggac gtggcattcg aatgtaccag
      961 cgtgaacaaa gtgttggata ctttggtcga agcctgcaaa cctgccgcca atttggttat
     1021 cgtatccatc tggagccacc ctgcaaccgt caacgtccac agcgtcgtga tgaaagagct
     1081 ggatgtgcgc ggcacgattg cctactgtaa cgaccacgcc gaaaccatca aactggtcga
     1141 agaaggcaaa atcaacctcg aacccttcat cacccagcgc atcaagctgg acgaactggt
     1201 ttccgaaggc ttcgagcgtc tgatccacaa caacgaatcc gccgttaaaa ttattgtgag
     1261 tccaaacctg taagcaaatt acatttgcaa cagcacaaat gccgtctgaa acgctcagac
     1321 ggcattttcc aacgaagcgc aacaggcgta ttgcccgaga gcagccgttt agcctatcat
     1381 tccgaacaaa cagaaacaaa cagaaggaaa atcatgggag attccgtact atccgccatc
     1441 caacaaacca tcacccagcg aaaatctgcc aatccgtccg aatcttacgt cgcacagctc
     1501 ttgcataagg gcgaagacaa aatcctaaaa aaagtgattg aagaggcggg cgaagtgttg
     1561 atggcatcca aagacaaaaa cccgtcccac ctggtttacg aagttgccga cttatggttt
     1621 cacaccatga ttcttctgac acaccacgac ctgaaggcgg aagacgtatt ggacgaactt
     1681 gcgcgccgcc aaggtttgtc gggcttggcc gaaaaagccg ctcgcacaga atcttgaatt
     1741 tatattaaaa tccgcacttt cccacattca atccgtctga ccgctgttca gacggcaacg
     1801 gagccgttat ggacaactgt attttctgca aaatcgccgc caaagacatt ccggcgcaaa
     1861 ccgtctatga agacggcgaa atggtttgtt tcaaagacat caaccccgct gctccggttc
     1921 atctgctgct gattcccaaa gtccatttcg attcgttggc acacgccgcg cccgaacatc
     1981 agcccctttt gggaaaaatg atgctgaaag ttcccgaaat cgccaaagcg gcaggactgg
     2041 cagacggctt caaaaccctg atcaacaccg gaaaaggcgg cggacaagag gtcttccacc
     2101 tgcatataca catcatgggc acacccgtat aaaccgttat ttcacaatca acccctaata
     2161 cttacttaag gatacatcat gggcagtttt tctctgacgc actggattat cgtactgatt
     2221 atcgtcgttt tgatattcgg caccaaaaaa ctgcgcaacg tcggcaaaga cctcggcggt
     2281 gcggttcatg acttcaaaca ggggctgaac gaaggtacag acggcaaaga agcccaaaaa
     2341 gacgatgtaa tcgaacacaa aaaagacgaa gacaaagcgt aatttatgtt tgatttcggt
     2401 ttgggcgagc tggtttttgt cggcattatc gccctgattg tcctcggccc cgaacgcctg
     2461 cccgaggccg cccgcaccgc cggacggctc atcggcaggc tgcaacgctt tgtcggcagc
     2521 gtcaaacagg aatttgacac tcaaatcgaa ctggaagaac tgaggaaggc aaagcaggaa
     2581 tttgaagctg ccgccgctca ggttcgagac agcctcaaag aaatcggtac ggatatggag
     2641 ggtaatctgc acgacatttc caatggtctg aagccttggg aaaaactgcc cgaacagcgc
     2701 acgcctgccg atttcggtgt cgatgaaaac ggcaatcccc ttcccgatgc ggcaaacacc
     2761 ctatcagacg gcatttccga cgttatgccg tccgaacgtt cctacgcttc cgccgaaacc
     2821 cttggggaca gcgggcaaac cggcagtaca gccgaacccg cggaaaccga ccaagaccgc
     2881 gcatggcggg aatacctgac tgcttctgcc gccgcacccg tcgtacagac cgtcgaagtc
     2941 agctatatcg atactgctgt tgaaacgcct gttccgcaca ccacttccct gcgcaaacag
     3001 gcaataagcc gcaaacgcgg tttgcgtccg aaacaccgcg ccaaacctaa attgcgcgtc
     3061 cgtaaatcat aaagagggca aatccggtgt ccgaaacaca aaacgaacaa cccgtccaac
     3121 cgcttgtaga gcatctcatc gagctgcgcc gccgcctgat gtggacggtt gtcggcatct
     3181 tagtctgctt tttcggcatg atgccgtttg cccaacaact ctatactttt atcgccaacc
     3241 cgctgatggc aaacctgccc aaagacacca gcatgattgc caccgatgtc atcgcaccgt
     3301 ttttcgtgcc ggtcaaagtt accctgatgg cggcattttt aatttcgctg ccgcatacgc
     3361 tctaccaaat ctgggcattt gtcgcccccg cactctacca aaacgaaaaa cgcctgatta
     3421 cgccgctcgt cctctccagc gtcagcctgt ttttcatcgg tatggcattt gcctactttt
     3481 tggttttccc cgtcattttc aaattccttg ccggcgttac ccctgtcggg gtcaatatgg
     3541 cgacagacat cgacaaatac ctctccttca tcttggggat gtttgtcgca ttcggtacaa
     3601 cgtttgaagt ccccattgtc gttatcctgt taaccaaaat tggtgtggta acaaccgaac
     3661 agctcaaacg cgcccgcccc tatgtgattg tcggcgcgtt tgtcattgcc gccatcatca
     3721 cgccgcccga tgtgatttca caaaccctgc ttgccattcc gctgattctc ttatacgaag
     3781 caggtatttg gttcggacgc tttttcacgc cacgttcaga acaggatggc gacatacagc
     3841 cgcctgcaac aacctgacac tatgccgtcc gaacctccgc ctcataccgc aacagattaa
     3901 ggaatacctt tgaataccct ctatttaggt tcaaacagcc cgcgccgaat ggaaatcctg
     3961 acacagttgg gctatcaggt cgtcaagctg cctgccaaca tcgacgaaac ggtcagacag
     4021 aacgaagacc ctgcccgtta cgttcaaagg atggcagaag aaaaaaaccg aaccgccctg
     4081 accctctttt gcgaaaccaa cggcacaatg cccgatttcc ccctgattac cgccgacacc
     4141 tgcgtcgttt cagccggcat tatattaggc aaaccccgct cccaagccga agcgatcgaa
     4201 tttttaaacc gattgtccgg caaacaacat accgtcctga ctgccgtctg catccgttat
     4261 cgcggcaaaa cgtcaagccg cgtccaaacc aaccgcgtcg ttttcaagcc cctgagttcg
     4321 gaagaaattt ccgcctatgt ccaaagcggc gaaccgatgg acaaagccgg cgcctacgcc
     4381 gtacaaggca taggcggcat ctttatccaa tctatcgaag gcaacttcag cggcattatg
     4441 gggctgcccg tttatgaaac cgtttcgatg ttgcaggatt tgggataccg taccccacct
     4501 ttcatccgag cctaacccgt aaagaccgcc gtgaacagac aaaccactta cctccttgcc
     4561 tctttcagcc tgatcgcact gataatcctg tccctttcct gggaactgtg gattgcaccg
     4621 ttgcgcccgg gcggttcgtg gctcgcgctc aaagccctcc ccctctgtct gccgctttca
     4681 ggtatcttga aaaagaaaat ctatacctac caatacagct ccatgctggt tctgatttat
     4741 tttgccgaag ccgttatgcg cctgttcgac gcctatccgg cagaacagat ttgcgccgcg
     4801 ctttccgcag tattcagcat catcttcttc atatcctgcc tgtcgttcgt caaacaatac
     4861 aaggaaacaa acaatgcccg ctgaaacgac cgtatccggc gcgcacccca ccgccaaact
     4921 gccgatttac atcctgccct gcttcctttg gataggcatc gtccccttta cctttgcgct
     4981 caggctgcaa ccgtcgcccg acttttacca cgatgccgcc gccgcagccg gcctgattgt
     5041 cctgttgttc ctcacggcag gaaaaaagct gtttgatgtc aaaatcccac ctatcagctt
     5101 ccttctgttt gcaatggcgg cgttttggta tcttcaggca cgcctgatga acctgattta
     5161 ccccggtatg aacgacatcg tctcttggat tttcatcttg ctcgccgtca gcgcgtgggc
     5221 ctgccggagc ttggtcgcac actacggaca agaacgcatc gttaccctgt ttgcctggtc
     5281 actgcttatc ggctccctgc ttcaatcctg tatcgtcgtc atccagttta ccggctggga
     5341 agacaccccc ctgcttcaaa acatcatcgt ttacagcggg caaggcgtaa tcggacacat
     5401 cgggcaacgc aacaacctcg gacactacct catgtggggc atactcgccg ccgcctacct
     5461 caacggacaa cgaaaaatcc ccgccgccct cggcgcaatc tgcctgatta tgcagaccgc
     5521 cgttttaggt ttggtcaatt cgcgcaccat cttgacctac atagccgcca tcgccctcat
     5581 ccttcccttc tggtatttcc gttcggacaa atccaacagg cggacgatgc tcggcatagc
     5641 cgcagccgta ttccttaccg cgctgttcca attttccatg aacaccattc tggaaacctt
     5701 taccggcatc cgctacgaaa ctgccgtcga acgcgttgcc aacggcggtt tcacagactt
     5761 gccgcgccaa atcgaatgga ataaagccct tgccgccttc cagtccgccc cgatattcgg
     5821 gcacggctgg aacagttttg cccaacaaac cttcctcatc aatgccgaac agcacaacat
     5881 acacgacaac ctcctcagca acttgttcac ccattcccac aacatcgttc tccaactcct
     5941 tgcagagatg gggatcagcg gcacgcttct ggttgccgca accctgctga cgggcattgc
     6001 cgggctgctg aaacgccccc tgacccccgc atcgcttttc ctgatctgca cacttgccgt
     6061 cagtatgtgc cacagtatgc tcgaatatcc tttgtggtat gtctatttcc tcatcccttt
     6121 cggactgatg ctcttcctgt cccccgcaga ggcttcagac ggcatcgcct tcaaaaaagc
     6181 cgccaatctc ggcatactga ccgcctccgc cgccatattc gcaggattgt tgcacttgga
     6241 ctggacatac acccggctgg ttaacgcctt ttcccccgcc actgacgaca gtgccaaaac
     6301 cctcaaccgg aaaatcaacg agttgcgcta tatttccgca aacagtccga tgctgtcctt
     6361 ttatgccgac ttctccctcg taaacttcgc cctgccggaa taccccgaaa cccagacttg
     6421 ggcggaagaa gcaaccctca aatcactaaa ataccgcccc cactccgcca cctaccgcat
     6481 cgccctctac ctgatgcggc aaggcaaagt tgcagaagca aaacaatgga tgcgggcgac
     6541 acagtcctat tacccctacc tgatgccccg atacgccgac gaaatccgca aactgcccgt
     6601 atgggcaccg ctgctacccg aactgctcaa agactgcaaa gccttcgccg ccgcgcccgg
     6661 tcatccggaa gcaaaaccct gcaaatgacc cgcgccggcg gatacggata ccgcccgaaa
     6721 tgtaaagctc catgcaagac attgcaaaaa acagcaaacc ggtagggaaa tacgctatca
     6781 aaaaacattg cagccgtgtt aagataaacc gtcaaacaat cttttcacgc cccgcccgaa
     6841 acagggtcgg ggcataccct tacgaaaagg aaacaccatg agccgcgtat tactcgtaga
     6901 tgacgatgcc ctgctgaccg aactgctgac cgaatacctg agcgccgaag gtctgaacgt
     6961 ccgcagcgtt cccgacgggg aagcaggcgt acaggaaatc ctgagcgggc agtacgatgt
     7021 agtcgtattg gattccatga tgcccaaaat gaacggcttg gatgtcttga aaaacgtacg
     7081 cgcccgaagc accgtcccca tcatcatgct gaccgccaaa ggcgacgaca tcgaccgaat
     7141 catcggcttg gaaatgggcg cggacgacta tgtccccaaa ccctgcacac cacgcgaact
     7201 cttggcacgc atcaatgcca tcctgcgccg cgcacaacac agcggcgaac agaacaacgc
     7261 acccaacagc atctccgtca gcgatgtcgt cctgtacccc gccaaacgcc aggcatccgt
     7321 caaagacatg ccgctcgaac tgaccagcac cgaattcaac ctgctcgaag tcctgatgcg
     7381 ccatgccgga caggtagtca gcaaagaaac cctgtccgtc gaagcactcg accgcaagct
     7441 ggcaaaattc gaccgcagta tcgacgtaca catctccagc atccgccaca agttgggcga
     7501 tgcctctctg attcaaaccg tacgcggctt gggctacctg tttgtcaaaa actgaagcaa
     7561 cgggcaaatg aaactgttcc aacgcatttt cgccacattt tgcgcagtta tcgtctgtgc
     7621 aatctttgtg gcgagttttt ctttttggct ggtgcagaac acccttgccg aaaaccagtt
     7681 caaccaacgc cgcaccatcg aaaccacttt gatgggcagc atcatttccg cattccgggc
     7741 acgcggggac gcgggcgcgc gcgaaatcct gacggaatgg aaagacagcc ccgtctcatc
     7801 gggcgtgtac gttatacagg gcgacgagaa aaaagatatc ctgaaccggt atatcgacag
     7861 ctacaccatc gaacgcgccc ggcttttcgc cgccggacac ccgcattcca acctcgtcca
     7921 tatcgaatac gaccgcttcg gcgaagaata cctgttcttc accaaagact gggacaaact
     7981 ccaagcccgc cgcctgccca gccccctgtt gatccccggc ctgccgctcg ccccgatttg
     8041 gcacgaactc atcatattgt ccttcatcat cgtcgtcgga ctgctgatgg cgtacatcct
     8101 cgccggcaat atcgccaaac ccatcagaat cttaggcaac ggcatggaca gggtggcaaa
     8161 cggagaactt gaaacccgta tctcccaaca ggtcgacgac cgcgacgacg aattgtccca
     8221 tcttgccatc caattcgaca aaatggtgga aaaactcgaa aaactcgttg ccaaagaacg
     8281 ccacctgctc caccacgttt cccacgaaat gcgctccccg cttgcccgta tgcaggcgat
     8341 tgtcggactg attcaggcgc agccccaaaa acaggaacaa tatctcaaac ggctggaagg
     8401 cgaactgacc cgcatggata cgctggccgg ggaactgtta accctgtccc gtctcgaaac
     8461 ttccaatatg gctttggaaa aagaaagcct gaaactcctg cccttcctgg gcaacctggt
     8521 agaagacaat caaagcattg cccagaaaaa cggacaaacg gttaccctgt ctgccgacgg
     8581 aaaaatcccc gaaaacacaa ccatccttgc caacgaaagc tacctgtacc gcgccttcga
     8641 caacgtcatc cgcaacgccg tcaactacag tcccgaaggc agcaccatcc tgatcaacat
     8701 cggacaagac cacaaacact ggataatcga cgttaccgac aacggccccg gcgtggacga
     8761 aatgcagctc ccgcacatct tcaccgcttt ctaccgtgca gactccagtg ccaacaaacc
     8821 cggaacagga ctggggcttg cattgaccca acatattatt gaacagcact gcggcaaaat
     8881 catcgccgaa aacatcaaac cgaacgggct gcggatgcgc ttcatcctac ccaagaaaaa
     8941 aaccggttcc aaaacagaaa aaagtgcgaa ctgaccataa tgccgtctga accggcttca
     9001 gacggcattg cacaaacagt taccccaaaa caaacacaca gcgggtgcat ctccaaaaat
     9061 cacgcacata ccgtccgcaa aggaatatat catgtcggca caaaccgatc ccggctactt
     9121 cttcatgccc aaccacatta tcctgatagg cgcgagcgaa cgtccgtaca gcctgggcga
     9181 acgcgtactc agcaacctgc tgagtacgcc ctttcaagga aaaatcaccc ccgtaaaccc
     9241 gcgccaccac accatagccg ggcttcccgc ctacaccagc ctcaacaaaa tccccggcaa
     9301 tgcagacctg attattgccg ttaccctacc cgacagttac gacaccctct tcaaaacctg
     9361 ccgcaaaaag cagctccgac acatcatcct catacaggac tgggacagcc tgtctgccgc
     9421 agaactgcac accgccgaaa ctgccatccg caaacaccac ggcaacggac tcaacatcac
     9481 cgcctgcacc accgcaggca tccaactgcc ctcactcgga cttaacatca gtacccaaga
     9541 cggatacgcc gcaggccata ccgccatact gaccggcaat gccgccgtca gccgccaaat
     9601 cgacaacatc ctgaacaaac tccgtcaagg cacatcccgc cacatcagcc tgcatcccgg
     9661 catcagcccc atcacatccg ccgattggct caaccgcttc ggacacagcc tgcacaccaa
     9721 aaccgccgtc ctacaccaca accctgaaga gaatcagcgc aaactgttca gcgcaatccg
     9781 ccaatttacc cgccatacgc cgctgattct ccacatcacc tgccgcacga cagaaaccga
     9841 ccgtgccgta ctgcactgcc tcgcccgaca ctgcaacttc ctcatcagtt tcaacgccga
     9901 cgacctcgaa gccgcactgc gcgcccaact gtccgacctt ccacccctgt cccgactcga
     9961 catcctgtcc gacacgcctg ccgaatggct gaacgcgcac gcgccaaaaa ccctcgccct
    10021 ccactttccc aaccttcccc accacatccg caacggacac ctgaccggca cacccacacc
    10081 ctcaatctgc cacgacatcg cctcacgtca gcttgcccgc cccgacaccc aagccgtcct
    10141 gaccatcctc agcccctccg gacacgagga ttacaaaaaa acagcacgcg cccttatccg
    10201 cctgtccgaa cagagcgcca aacccctgct cgtcagcagc cccttttcag acggcataac
    10261 acatttcgac acccccactc aggcaatccg caccctttcc taccgcaaca ccgccgccgc
    10321 cctgaaacag gcacagctcg acattgcacc gccgcagcca tgccgtctga aaacaccgca
    10381 accccaaaac atcaaaaaag ccctcgaagc cgccgacccc gccctaatcg ccgaagccct
    10441 gcacctcccc ccctaccggc acaccaccca taacgccgtc caattccaat tcggcagcca
    10501 ctccctctat ggcgacatcc tgaccgcacg ttgcaacgga caaaccactg ccgtactccc
    10561 gccgtttacc acgctcgaca gccgccacct tgcccgcttt gccgaactcg acggcacaca
    10621 aaccctcgac cagttcctgc acacactgac catcattccc gaataccgcc aacacattct
    10681 cggcatcacc ctcaacctaa acggcggaca atacagcagc gatttcatct taaagacacc
    10741 cgaaacacac gacacgccca aacgcaagaa cacaggcaaa gccgcccaaa ccctcgaaca
    10801 tgccgccgca aaaatgcaga gtgccgccgc atacctgaaa cacaaaaacc cgacagccgc
    10861 cgaatttctc cgccacacaa gcgaagccgc cgcagaactg ctcggcagca aaaccgaaac
    10921 cggagcagcc gtacccaacg tacttgcccc ctatcccgca gcatacccca aaacactgtc
    10981 cctaagaaac aacacgaccg ttaccattac ccccattttg cccgaagacg cagaagccaa
    11041 acagcagttc gtccgcagcc tcggtcccga agcacggtac acacgcttca tgacccacac
    11101 caacgaactg cccgcagcca cgttggcacg cctgtgcaac cccgattacc actgtgaagc
    11161 cgcatggacg gcaaaggatg ccgacagcaa catcgtcgcc gtcgtccgcc acagccgcct
    11221 gaaccgcaac gaatgcgaat tcggcatcac actggcggaa catatgcgcg gcagcggact
    11281 ggcacagaaa atgatggaac tcatcatcca aaccgccgca cagcaaggct accggactat
    11341 gagtgccgac attctcaaaa ccaatacccc catgatcaaa cttgctgaaa aatcaggatt
    11401 taccctcaag gaatcggaca ccgaaaaaaa cctgtaccgc gcatatctga accttgcggc
    11461 agacaaaaca acagaaaaaa caaataaaaa cttgcgcacc gaccacaaaa taacctaaaa
    11521 tcggcagttt ccatatatcc gattttctca aaaggactca aaatggtagt tatccgtttg
    11581 gcacgcggcg gctcgaaaca ccgccccttc tacaacgtca tcgttactga ctcacgcagc
    11641 cgccgcgacg gccgcttcat cgaacgcgta ggcttctaca accccgtagc caacgaaaaa
    11701 caagagcgcg tccgcctcaa tgcagaccgc ctgaaccact ggattgcaca aggcgcgcaa
    11761 gtcagcgact ccgttgcaaa actgattaaa gaacaaaaag ccgcctaatt cacatttgcc
    11821 gccatgacag acactcaaaa ccgggtagcc atgggctaca tcaaaggcgt attcggcata
    11881 aaaggctggt tgaaaattgc cgccaacacc gaatattccg acagcctttt ggactacccc
    11941 gagtggcatt tggtcaagga cggcaaaacc gtcagcgtta cccttgaagc cggaaaagtc
    12001 gtcaacagcg aactccaagt caaattcgaa ggcataaacg accgcgactt ggcattctca
    12061 ttgcgcggtt acaccatcga aataccccgt gaagcattcg ccccgacaga agaagacgaa
    12121 tactactgga cagacttggt cggcatgacc gtcgtcaaca aagaccatac cgttttaggc
    12181 aaggtaagca acctgatgga aaccggcgca aacgacgtat tgatgattga cggagaacac
    12241 gggcagattc tgattccgtt cgtttcccaa tatatcgaaa ccgtcgatac cggcagcaag
    12301 attattactg ccgactgggg tttggactac tgatgcttat ccaggcagtt accattttcc
    12361 ccgaaatgtt cgacagcatt acccgctacg gcgtaacggg acgcgcgaac agacagggaa
    12421 tctggcagtt tgaagcagtc aatccccgaa agtttgccga caacagattg ggctacatcg
    12481 acgaccgccc gttcggcggc ggcccgggaa tgattatgat ggctccgccg cttcatgcag
    12541 caatagaaca cgccaaagca caatcctccc aagctgcaaa agtcatctac ctcagccccc
    12601 aagggaaacc gctgacacac caaaaagcgg tagaactggc agaacttccg catctgattc
    12661 tgctgtgcgg acggtatgag ggcatagacg aaaggcttct gcaaagcagc gtcgatgaag
    12721 aaatcagcat cggagacttc gttgtttccg gcggagagct tcccgccatg atgctgatgg
    12781 atgcggtatt gaggctcgta cccggcgtat tgggcgatat gcagtctgcc gaacaagatt
    12841 cgttctcaag cggcattttg gactgccccc actacaccaa acccttagaa tttcaaggca
    12901 tggctgttcc ggaagtatta cgttccggca atcatggctt gatagcggaa tggcggttgg
    12961 aacaatcgct gcgccgcacc ttggagcgca gacccgatct tttggaaaag cgcagtttaa
    13021 tcccaaagga atcccgcatc ttgaataaaa tcctacaaga gcaacgggaa atccaatcat
    13081 aatttaggaa aaaaacaatg aacctgattc aacagctcga gcaagaagaa attgcccgcc
    13141 tgaacaaaga aatccccgaa ttcgcaccgg gcgacaccgt agtcgtatcc gtacgcgtcg
    13201 tggaaggtac ccgcagccgt ctgcaagcct acgaaggcgt tgtcattgca cgccgcaacc
    13261 gtggtctgaa cagcaacttc atcgtccgta aaatttccag cggcgaaggt gttgaacgta
    13321 ctttccaact gtactctccg accgtcgaaa aaatcgaagt caaacgccgt ggcgacgtac
    13381 gccgtgccaa actgtactac ctgcgcggtc tgaccggcaa agctgcacgc atcaaagaaa
    13441 aactgcctgc acgcaaaggt tgattcaaac ccgttttccc caatgccgtc tgaaccttct
    13501 gacggcattt cttattgccg caacatccgc aacggaacag caccaagaag cacacccctt
    13561 ccggcacatc ccgaatcccc gtccgaaccg aacaaaattt acagaccaaa cgttatacag
    13621 tatcatatca gccatataat aataaccgct ttaccgcctt ccctttcaaa accgcaaatc
    13681 atcatgacac cttccctttt actatcagga ttgaccttcc gcctcgtcct tgccctgatt
    13741 gccgtatccc ttttatgggg cgtttacttt tgggcagtat ccgcatgagc atcattgtcg
    13801 aaaacctgac cgtcagctac cgccgccgac ctgccgtgca ccatgtggac attacttttg
    13861 aagaacatag tatgtgggcg gttttcggtc ccaacggcgc agggaaatcc acctttctca
    13921 aatccttaat gggattgcag cctatcgata cgggcagcat ccggctggac ggattgaccc
    13981 gtcagaacat tgcctacctt ccccagcagt ccgacatcga ccgctcgcag cccatgaccg
    14041 ttttcgactt ggcggcaatg gggctatggt atgaaatcgg ttttttcaga ggaataaatg
    14101 ccgttcaaaa acaacgcgtt cacgaatctt tggaacgcgt cgggatgcgg cagtttgccg
    14161 gccgccaaat cgcccacctc tcaaacggac aattccagcg tgtccttttt gcccgaatgc
    14221 tggttcaaaa cgccaaattc ctacttctag acgaaccttt caacgctgtt gatgcacgga
    14281 cgacctacga acttctcgac gtattgcaga aatgccattg cggcggacac gccatcatcg
    14341 ccgtactgca cgattacgaa caagtccgtg cctactttcc caataccctg ctgctcgccc
    14401 gcgaaaaaat tgcggcaggc gcaaccgaga ccattctgac agaaccctac ctcgcccaag
    14461 ccaacgccaa aatgcagcaa caggaaagcc ccgagtggtg cgcctcataa atgccgtctg
    14521 aaaccatgtc cccgtaccaa acactatgaa cccctacgac ctgcttatca caccctttgc
    14581 agaattcgac tttatgcgct acgccctcgt atccgtcttc tgcctgtccc tcagtgccgc
    14641 acccgtcggc gtattcctcg tcatgcgccg tatgagcctg ataggcgacg cattgagcca
    14701 cgccgtcctg cccggtgccg ccgtcggcta catgtttgcc ggcttaagcc tgcccgccat
    14761 gggtttgggc ggcgtagccg caggtatgct gatggcactg cttgccggac tcgtcagccg
    14821 cttcaccacc ctgaaagaag atgccaactt tgccgccttt tatctcagca gcctcgccat
    14881 cggtgtagtc ctcgtcagca aaaacggcag cagcgtcgat ctgctgcatc tgcttttcgg
    14941 ctctgtactt gccgtcgata ttcctgccct gcaactcatc gccgccgtat ccagcctcac
    15001 gctcattacc cttgccgtca tctaccgccc gctcgtactc gaaagcatcg accccctgtt
    15061 tctcaaatct gtcggcggca aaggcgggct ttggcacgtc ctctttctcg tcctggtcgt
    15121 catgaacctc gtatccggct ttcaagccct cggcacactc atgtccgtcg gactcatgat
    15181 gctgccagcc attaccgccc gcctgtgggc gaagcacatg ggcgcactca tcctcctatc
    15241 cgttctgaca gccctgctgt gcggcttgag cgggctgctc atttcctacc acatcgaaat
    15301 cccttccggc cccgccatca tcctctgttg cagcgtcctt tatctctttt ccgtcatact
    15361 cggcaaagaa ggcggcattc tgaccaaatg gctcaaaaac caccgccacc acaccacctg
    15421 accccgcaag ccgcttgaac aaacaggcgg cttgttttac cataacagat gttacgttat
    15481 atcacaggag aacagttatg aaacacccca aactcaccct tattgccgca ttgctaacca
    15541 ccgccgcaac tgccgccccc ctgcccgtcg taaccagctt cagcatttta ggcgacgtag
    15601 ccaaacaaat cggcggagag cgcgtatcca tacaaagttt ggtcggagcc aaccaagata
    15661 cgcacgccta tcatatgacc agtggcgaca ttaaaaaaat ccgcagtgca aaactcgtcc
    15721 tgctcaacgg cttaggactt gaagctgccg atgtacaacg tgccgtcaaa cagagcaaag
    15781 tatcctatgc cgaagcgacc aaaggcatcc aacccctcaa agccgaagaa gaaggcggac
    15841 accatcacga ccacgatcat gaccacgatc atgaccacga aggacaccac cacaaccacg
    15901 gcgaatatga cccccacgtc tggaacgacc ccgtcctcat gtccacctat gcccaaaacg
    15961 tcgccgaagc cctgataaag gccgaccccg aaggcaaagt ttattatcaa caacgcttgg
    16021 gcaactacca aatgcagctc aaaaaactgc acagcgacgc acaagccgca tttaacgccg
    16081 tccctgccgc caaacgcaaa gtcctgaccg ggcacgatgc cttttcctat atgggcaaac
    16141 gttaccatat cgaattcatc gccccgcaag gcgtgagcag cgaagccgag ccttcagcca
    16201 aacaagtcgc cgccatcatc cgccaaatca aacgcgaagg catcaaagcc gtattcaccg
    16261 aaaatatcaa agacacccgc atggttgacc gtatcgccaa agaaaccggt gtcaacgtca
    16321 gcggcaaact gtattccgac gcactcggca acgcgcccgc agacacctac atcggaatgt
    16381 accgccacaa catcaaagcc ttgaccaacg cgatgaagca ataaaaccgt ttgattggtt
    16441 tgaatttgaa aaggcaaaag gccgtctgaa aattatagtg gattaacaaa aatcaggaca
    16501 aggcgac
//
LOCUS       DAXHNE010000031        16440 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.031, whole genome shotgun sequence.
ACCESSION   DAXHNE010000031 DAXHNE010000000
VERSION     DAXHNE010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16440
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.031"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(215..3004)
                     /gene="ileS"
                     /locus_tag="ACFM4Z_000781"
     CDS             complement(215..3004)
                     /gene="ileS"
                     /locus_tag="ACFM4Z_000781"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFC6020849.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQVARQKKDFIRLGVLGDWDRPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKISPAIDVAYPFKDTAA
                     LAAAFGLAGFEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGISILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYIGETLRVAGMRVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVALKIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDGEGKKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYALVLARRLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSHARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMASYL
                     NALGEELRFALLVSKAEVKVGDELAVAAKASDGEKCERCWHYTHDVGAVAGHETICKR
                     CAGNVGGEGETRHYA"
     gene            complement(3145..4065)
                     /gene="ribF"
                     /locus_tag="ACFM4Z_000782"
     CDS             complement(3145..4065)
                     /gene="ribF"
                     /locus_tag="ACFM4Z_000782"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFC6020850.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADT
                     RGLPVVTVVFEPQPKEFFALRTGRMPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMAELKRQIAADMEAAKCW"
     gene            complement(4364..5659)
                     /gene="tyrS"
                     /locus_tag="ACFM4Z_000783"
     CDS             complement(4364..5659)
                     /gene="tyrS"
                     /locus_tag="ACFM4Z_000783"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFC6020851.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     NQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLHQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAIEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEVLVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLTDEHKRFGKYTILRRGKRNHALLVWK"
     gene            complement(5722..7590)
                     /locus_tag="ACFM4Z_000784"
     CDS             complement(5722..7590)
                     /locus_tag="ACFM4Z_000784"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC6020852.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKDWLPGGFLGVDIFFV
                     ISGFLMTTILHREMSGGGRFSLKAFYIRRIKRILPAFFAVLAATLAGGFFLFTKDDFF
                     LLWKSALTALGFASNLYFARGKDYFDPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVA
                     RKSLRVQFGFLAALCALSLAASFIPSALDKYYLPHLRACELLIGSLTAVWMRCRQPAV
                     GRRCAAVGALFAVCILSTCLFSYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPLKKF
                     FQSKITVAAGLISYSLYLWHWPILAFMRYIGPDNLPPYSPAAAVVLILLLSLFSYHCI
                     EKPFKKWQGSFAQSVLWIYALPMLILGAGSFFAMRLPFMAQYDRLGLTRSNTSCHNNT
                     GKQCLWGDTEKQPELLVLGDSHADHYKTFFDAVGKKEKWSATMVSADACAYVEGYASR
                     VFQNWAACRAVYRYAEEHLPRYSKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRMLHK
                     LSSEKQAVYLMADNLASSYNVQRAYILSSRIPGYRQALRPDDESTLKANARIRELAAK
                     YPNVYIIDAAAYIPADFQIGGLPVYSDKDHINPYGGTELAKRFSEKQRFLDTRHNH"
     gene            complement(7956..9047)
                     /gene="ychF"
                     /locus_tag="ACFM4Z_000785"
     CDS             complement(7956..9047)
                     /gene="ychF"
                     /locus_tag="ACFM4Z_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFC6020853.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVSEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDGEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(9160..10836)
                     /locus_tag="ACFM4Z_000786"
     CDS             complement(9160..10836)
                     /locus_tag="ACFM4Z_000786"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFC6020854.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLLQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDIGDLKE
                     RLGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLDALEKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIESQTNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            11011..11442
                     /locus_tag="ACFM4Z_000787"
     CDS             11011..11442
                     /locus_tag="ACFM4Z_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC6020855.1"
                     /translation="MGSNAWLFWALASAGFASLTAIFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGLNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLNERPSTQEWIGLGLVTAGVLVLALKR"
     gene            complement(11688..12383)
                     /gene="murU"
                     /locus_tag="ACFM4Z_000788"
     CDS             complement(11688..12383)
                     /gene="murU"
                     /locus_tag="ACFM4Z_000788"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HFC6020856.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVVNGDVLTDIDFTAAFQAASSLPEHISAHLWLVENPPHNPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(12399..12899)
                     /gene="hpaC"
                     /locus_tag="ACFM4Z_000789"
     CDS             complement(12399..12899)
                     /gene="hpaC"
                     /locus_tag="ACFM4Z_000789"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042537 - benzene-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HFC6020857.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRKARIIPILSENGSLCINTLADEHQDVAEHFAGLTNLSPEERFAY
                     HIWHYGKTGQPEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            complement(13014..13454)
                     /gene="nadR"
                     /locus_tag="ACFM4Z_000790"
     CDS             complement(13014..13454)
                     /gene="nadR"
                     /locus_tag="ACFM4Z_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HFC6020858.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            complement(13797..14306)
                     /locus_tag="ACFM4Z_000791"
     CDS             complement(13797..14306)
                     /locus_tag="ACFM4Z_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HFC6020859.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWIRKPEEPAGAAGRPLT
                     LLSGQRLTLGQFSRDRTVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            complement(14385..14460)
                     /locus_tag="ACFM4Z_000792"
     tRNA            complement(14385..14460)
                     /locus_tag="ACFM4Z_000792"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14425..14427),aa:Lys,seq:ttt)
     gene            14676..15755
                     /gene="apbC"
                     /locus_tag="ACFM4Z_000793"
     CDS             14676..15755
                     /gene="apbC"
                     /locus_tag="ACFM4Z_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFC6020860.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIASETADRIQEILMPETGDTHIHLSIDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICTNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIAKIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
BASE COUNT         3744 a         4502 c         4433 g         3761 t
ORIGIN      
        1 gaaatccgtc attcccgcac aggcgggaat ctatcggaaa tgactgaaac ctcgagattc
       61 tagattccca ctttcgtggg aatgacggtt cagttgcatt ccgacaacac cgcaatcttg
      121 aaacccctcc gccgttataa agacaaatcg cggtacaaaa aatgccgtct gaaatgctgt
      181 ttggcggttt cagacggcat ttgctcaaac tttatcaggc gtaatggcgc gtttcgcctt
      241 ctccgccgac attcccggcg cagcgtttgc agatggtttc atggcctgca actgcgccca
      301 catcgtgggt gtagtgccaa cagcgttcgc atttttcacc atcgctggct ttggcggcaa
      361 cggcaagttc atcacctact ttcacttcgg ctttagacac cagcagggca aagcgcaatt
      421 cttcgcccaa agcgttcagg tagctggcca tttcttccgg cgcagtgatt tcggcttccg
      481 cctgcaagga cgaaccaacg gttttgtcgg cgcgcaaagg ctcaatggcg gcggttaccg
      541 cttcgcgggc ttcgcggatt gccgtccatt ttttcaccag ttcggcttcg gctttttcgt
      601 tgatggtcgg gaactcgtgc caagtatgga aaaggacgct gtcttcttcg ccgccgccga
      661 tgatgtccca cgcttcctcg ccggtgaagc acaaaatcgg tgcaatcaag agaaccaaac
      721 tgcgtgtgat gtgatacagg gcagtttgtg cgctgcggcg tgcatggctg tctgctttgg
      781 tggtatagag gcggtctttc aggatgtcga ggtagaacgc gcccaagtct tccgagcaga
      841 aagaaacaat gtcttttacg gcgaagtgga aggcgtagcg cggatagtaa tcgcctgcca
      901 gacgctcttg cagccgacgc gccaacacca gggcatagcg gtcgatttcc accatatccg
      961 cctgttgcac ggcatcttca atcggattaa agtcgctcaa gttggcaaac aaaaagctca
     1021 aggtattgcg gatacggcgg tagctttcgg ttacgcgctt aaggatttct ttagaaatcg
     1081 ccaattcgcc gctgtaatcg gtagatgccg cccacagacg caggatgtcc gcgccgaatt
     1141 cgttatagac ttcttgcggc gcgacgacgt tgccgatgga tttcgacatt tttttgcctt
     1201 cgccgtctac aacaaaaccg tgggtcagca gctgtttata cggtgcgcgt cccattgatg
     1261 aggcgcagcc ggtcagcatg gacgattgaa accagccccg gtgctggtcg ctgccttcga
     1321 gatacaaatc cgccggccat tccaattctt cgcgttgttt cacaacggaa taatgggtcg
     1381 aaccggaatc gaaccatacg tccatcgtat cggggagttt gtcgtaatgt tcgcaatctt
     1441 ccgcgcttaa taattcgctt ttatcgaggg agaaccaagc ctcgatgcct ttttcttcga
     1501 ttttcagggc aactttttcc aaaagttcgg cagagttcgg atgcagctcg cccgtttctt
     1561 tgtgaacaaa gaaagtcatc ggcgtgcccc aatagcgttg gcgtgaaacc acccagtcag
     1621 gacgaccttc aatcatggct tccaaacgcg cgcgacccca agacgggaag aattcggtgt
     1681 cgtccacggc cttgatggct ttgtcgcgca gggttttgcc gtcggcaccg gctttgtcca
     1741 taccgacaaa ccattgaccg gtcgcgcggt aaatcagcgg cgttttgtgc cgccagcagt
     1801 gggcgtagct gtgttcgatt ttactgcttg ccaaaaggtt gccggtttct tccaaccatt
     1861 gcaggatgac ggggttcgcc tcccaaacgc gcataccggc aacgcgcagc gtttcgccga
     1921 tgtatttgcc ttcggcgttg acaggattgt aaagctcgat gccgtatttg ttgcagacgg
     1981 cgtagtcttc caaaccgtgc gcgggcgcag tatgcaccaa gccggtaccg gcatcggtgg
     2041 taacgtgttc gccgttaagc atgggaatat cgcgttcgag gaacggatgg ttcatgtgca
     2101 ggttttccag cttgtcgccg gtggtttcgg caagaataga aatgccgtct gaaaagccgt
     2161 agcgtttcaa agcgtcttcc gccaaatctt tggccaatac caatttgcct ttgggcgtat
     2221 cgattaattg atacaccacg tctgcacccg ctgaaacagc ttggctcgca ggcaaagtcc
     2281 aaggcgtggt tgtccaaata acggcaaacg ctttgccttc gaaaccagcc aaaccgaatg
     2341 cggcggcaag cgcggcggtg tctttaaacg gataggcaac gtcaatcgcg ggcgagattt
     2401 tgtctttata ttccacttcc gcttcggcca gcgaagaacc gcagtccaag cagaattgaa
     2461 ccggtttcgc accccggtag agatagccgg atttgtagat ttcgccgagc atacgcacgg
     2521 tatcggcttc ggttttgaaa tccatggtca ggtaaggacg gtcccaatcg cccaacacgc
     2581 ccaagcggat aaagtctttt ttctggcggg caacttgttc ggcggcatat tcgcggcaca
     2641 attcgcggaa acgcgctttg ggcatgtctt tgccgtgcag tttttctacc atcacttcga
     2701 tgggcaggcc gtggcagtcc caacccggca cataaggcgc gtcaaaaccg gcttgggttt
     2761 tgctgcggat aatgatgtct ttgagaattt tattgacggc atgaccgatg tggatgtcgc
     2821 cgttggcata cggcgggccg tcgtgcagga tgaatttcgg acggcctttg gcgatttcgc
     2881 gcagtttttg gtagcgtttt tgctcgtacc agcttttcag ccatgcaggc tcgcgcttgg
     2941 caagattgcc gcgcatcggg aacgggcttt cgagcaggtt gacggttttg ctgtaatcgg
     3001 tcatttttta atctctattg ttacaatatt tcggttcaga cggcattgcg cctcaaacag
     3061 tatttcacaa cgggaaaccc ctatgccgtc tgaacagtta aaagattgat tgtagcccaa
     3121 tcggatggtt tgtataaggt ttttctacca acactttgcg gcttccatat cggctgctat
     3181 ctgccttttc agttctgcca taccgtcaaa cttttcttca tcgcgcagtt tgtgcaggaa
     3241 gcggacgttc agccgttgtc cgtacaggtc gccttgaaag tcgaacaggt ggacttcaag
     3301 cttttgagaa cagccgctat caacggtggg attgaaaccg aagctcgcca cgccgcgccg
     3361 cgtgccgaat gcgccgtccg cttcgacgac gaacacgccg ccgagtgcat aacggtggcg
     3421 gggcaggcgg atgttggcag tcggggcgtt taaggtgcgt ccgagttttc tgccgtgcac
     3481 caccctgccg ctcaagacgt agtcgtgtcc caaaagtttt ttcgcatagg caaggttgcc
     3541 gtctgaaagg gcttgtcgca cggcggtact gctggtgcgg atgtcttcga cgatgacgga
     3601 aggcgtacgc tcggtctgca tatcgggctg ttgtgccaaa agttcaaaac agccttcccg
     3661 ccccgcaccg aaacggaaat catcgccgac gagcaaataa cgcgtattca aggtttgacg
     3721 cagcaggcgg tcgataaacc cttgcgcgga tatttcggaa aaattttgat cgaaacgcaa
     3781 aacccagacg gcatcgacac agcctgtgcc ttccaataat tcgagcttgg tgcgcagggg
     3841 gctgatccga cacggtggca tcctgccggt gcggagtgcg aaaaattctt tgggttgggg
     3901 ttcgaaaacg acggtcacga cgggtaatcc gcgcgtatcg gcttcgaggc ggagtttttg
     3961 gaggatgtgt ttgtgtccaa ggtgtacgcc gtcgaaattg cctatggtta cggcggcccc
     4021 gtgcggaaag tcgggcgcgt tgtgccgccc cagcctgatt ctcattgttg cgttcgggta
     4081 tgtggtgaaa caggcggtca ttgtaaacgg tattgcggtt tatagacagt gtgccgcccg
     4141 ttacgcccgc ccgacgctgg aaaagcaggc aaatttcccg ccgccgaacg cgccaaacgc
     4201 ataaagcgtg catcaggcgc gatcctatgt gttgaaacat cgccccaaac ctgatgcaac
     4261 atcagtgaaa atcgttcttt tttaaccagg taaaccgaga caaacaaccc tccgccgtca
     4321 ttcccgcgca ggcgggaatg acggcatttc tgcggcaatc ggattatttc caaaccaaaa
     4381 gcgcgtggtt gcgtttgccg cgccgaagga tggtgtattt gccgaaacgt ttgtgttcgt
     4441 cggtcagcag gcaggcatcg tcggggcgtt cggcggcgtg gttggggttg ttggcttcgg
     4501 caggtttgcc gttgagcaaa accgctttgc tgttcacaaa gccgcgcgct tctttattgg
     4561 aggatgccaa accggttttt accaagactt cgacgacatt gatgccgtct gaaacttcaa
     4621 atgcaggcag accgtcgagg gcgagctgct cgaagtcgct ttcggtcagg ctgctttggt
     4681 cttcggcaaa caggctttcg gaaatgcgtt gcgcggcggc aagggcttct tcgccgtgaa
     4741 tcaggcgggt catttcttcg gcgaggatgc gttgcgcttc gggcttgctg ccgcttgcct
     4801 tgtctttggc ttcgatggca tcgatttctt cgatggacag gaaggtaaag tatttcagga
     4861 atttatacac atcggcatcg gcgactttca gccagaattg gtagaactga tagggcgagg
     4921 tttttttcgc gttcagccat accgcgccgc cttcggtttt gccgaatttg gtaccgtctg
     4981 atttggttac caaaggcagg gtcagaccga atacttgttt ttggtgcagg cggcgggtca
     5041 ggtcgatacc ggcggtgata ttgccccatt ggtcggagcc gccgatttcc aaaaccgcgc
     5101 cgtggcgttt gttcaactcg gcgaagtcgt aaccttgcag cagggaatag gcgaactcgg
     5161 tgaaggaaat gcctgcgccg tcgcggtcga tgcgctgttt gacggattct ttgttcagca
     5221 tggcgttgac ggagaaatgc ttgccgatgt cgcgcaggaa gtcaaggcag ttcatgctgc
     5281 cgaaccagtc ggcattgttc gccataatgg cggcatttcc gccttcaaag ctcaagaaag
     5341 gggttaattg gttgcggata ctttccaccc agccggcaac agtttcggcg gaattcaagc
     5401 tgcgttcggc ggctttgaag ctggggtcgc cgatcatacc ggtcgcgccg cccaccaaag
     5461 caatcggcgt atgccccgcc tgttggaagc ggcgcaatgc caatacgggc agcaggtgtc
     5521 cgatgtgcag gctgtcggcg gtcgggtcga agccgcaata aagggcaatt ttttgttcgt
     5581 tcaacaaagc gtctaaggct tcgatgtcgg tggtttgcgc gataaggccg cgcgattgca
     5641 ggtcttggat gacgctcatc ggtctctttc aaaaaaaatt agcgtttttg caaaccgccg
     5701 attgtaacaa atttaagcga atcaatggtt atggcgcgta tcgagaaacc gttgtttttc
     5761 ggaaaaacgc tttgccaatt ccgtgccgcc gtaagggttg atgtggtctt tgtccgagta
     5821 aaccggcaat ccgccgattt gaaaatctgc ggggatatag gcggcggcat caataatata
     5881 gacgttgggg tatttggctg ccaattccct gatgcgtgca ttggctttca gggtgctttc
     5941 gtcgtccggg cgcagggctt ggcggtaacc cggtatgcgt gaagacaaga tataggcgcg
     6001 ctggacgttg taagacgagg caaggttgtc cgccatcagg taaacggctt gtttttcgga
     6061 cgagagttta tgcagcatac ggtcgaattt ttggaaaaaa ccggcatcat aggcaaggga
     6121 gcggctgttt tcgggcattt ggctgcccca gcgcatcgcc aaaaccactt ttgaataccg
     6181 gggcaggtgt tcttcggcat agcgataaac ggcgcggcag gctgcccagt tttggaacac
     6241 acgggacgcg tagccttcca cataggcgca agcgtcggcg gaaaccatag tggcggacca
     6301 tttttctttt ttgcccacgg catcgaagaa tgttttgtaa tggtcggcgt gggagtcgcc
     6361 caaaaccagc agttccggct gtttttccgt atccccccat aggcattgtt tgccggtatt
     6421 gttgtggcag gaggtgttgg aacgcgtcag ccccaagcgg tcgtattgcg ccataaacgg
     6481 cagcctcatc gcgaaaaacg agcccgcccc caaaatgagc ataggcaagg cataaatcca
     6541 taaaacggat tgtgcgaacg aaccttgcca ttttttaaac ggtttttcga tgcagtggta
     6601 agaaaacagg gaaagcagca atatcaggac gaccgccgcc gccggcgaat aaggcggcag
     6661 gttgtccggg ccgatatagc gcataaaggc caatatcggc caatgccaca gataaagcga
     6721 ataggaaatc aaaccggcgg caacagtgat tttcgattgg aaaaattttt taagcgggtg
     6781 ttcgtaatga ttgaaataaa tcagcgcggc aacagccaga cagggaatca aagcggcggg
     6841 gcccgggaaa taggcggttt gttccgaata ggaaaacagg caggttgaca atatgcacac
     6901 ggcaaacaat gcgccgacgg cggcacagcg tctgccgacg gcaggttgcc ggcagcgcat
     6961 ccacacggcg gtcagcgatc ctatcagtaa ttcgcaggcg cgcaggtggg gcaggtaata
     7021 tttatcgagc gcggaaggta taaaggaggc ggcaaggctt aaggcacaca gtgcggcaag
     7081 gaagccgaac tgtacgcgca ggcttttgcg ggcgacaagc agcagcagta tcggaaagac
     7141 aaagtaaaat tgttcttcga ccgacaaaga ccagatgtgc agcaggggct tttcttcctg
     7201 cgcgggatcg aaataatcct tcccccttgc aaaatacagg ttagaggcga aacccaaggc
     7261 ggtcagcgcg gatttccaca aaagaaagaa atcatctttg gtgaataaaa agaagccgcc
     7321 tgccagcgtt gccgccaata cggcgaaaaa tgcgggcaga atccgcttga tgcggcggat
     7381 ataaaatgcc ttcagggaaa acctcccccc ccccgacatt tcgcggtgaa gaatcgtcgt
     7441 catcaaaaag cctgaaatca caaagaatat atcgacaccg agaaacccgc ccggcagcca
     7501 atccttttcg atatggaaca cgatgacgga caagacggcg gcggcgcgca atgtgtcgat
     7561 gtccgggcgg tagggtaagg cttggctcat aatgttttta tagtggatta acaaaaacca
     7621 gtacggcgtt gcctcgcctt gccgtactat ctgtactgtc tgcggtttcg tcgccttgtc
     7681 ctgatttaaa gttaatccac tatactcgaa acgcggcggc gcaaatgccg tctgaaaggt
     7741 cattttgtac gggatattcg gaatgccgga cggatttttg caacagcggg gcaggcggtt
     7801 tgttttgcag gaatcgggga gggcaaatcg gaaatgcgga cgggatttta ttttgatgca
     7861 gccgcattcc ggcggtatgg aaacgccgaa aaccaccaaa accggcttca gacggcattt
     7921 ctggcaaagc cgtctgaaaa cctgccgcat ttgggttaca cgttaaacaa aaagtgcatc
     7981 acatccccat cctgcacgac gtattcctta ccttccacac gcattttgcc ggcttctttg
     8041 gctttggctt cgccgccgag cgagacaaag tcgtcgtaag aaatgacttg ggcgcggatg
     8101 aagccgcgtt cgaagtcggt gtgaatcacg ccggcggctt gtggggctgt gtcgcctttg
     8161 tgtatcgtcc acgcgcggac ttctttgacg ccggcagtga agtaggtttg cagccccaaa
     8221 aggtcgtaac cggcgcggat caggcggttc aggcccggtt cttccaagcc catttcggcg
     8281 aggaactcgg ctttttcacc gtcttccaac tcggcgattt cgctctccat cgcggcgcac
     8341 acggcgacga cgggggcgtt ttcttttgcc gccaattctt tcaggcggtc gaggtgcggg
     8401 ttgttttcaa aaccgtcttc ggaaacgttg ccgacgtaca tggcaggttt ggcggtcagc
     8461 aggaacagcg gtttgagcat cgcgcgttct tccgcgtcca aaccgaagga acgcacgggt
     8521 ttgccttcgt ccagatgcgg cagcagtttt ttgcacaaat cgaccagctt ttgcgcgtct
     8581 ttgtcaccgg aacgggcgcg tttttcttcg cggacgatgg ctttttccac acttgccaag
     8641 tcggcaagcg ccaactctgt accgatggtt tcgatgtcgg caatcggatc gacgcggcct
     8701 gcaacgtgga cgatgttgtc gtcgtcaaag cagcgcacga cattcacaat cgcatcggtt
     8761 tcgcggatgt tggcaaggaa ctggttgccc aagccctcgc ctttgctcgc gcctgcaacc
     8821 aaaccggcaa tatcgacaaa ttcgacgatg gcaggctgca ttttttgcgg attgacgatt
     8881 tttgccaatt cggccatacg cggatcgggg acttcgacga tgccgacgtt gggttcgatg
     8941 gtacagaaag gatagtttgc cgcttcgata cccgattggg tcagcgcgtt aaaaagggtg
     9001 gatttgccga cgttgggcaa accgacgatg ccgcatttca aactcatgtt ttttcctgaa
     9061 aatagagaaa tttaacggcg gattatagca taccgccgcc cgcgttccga aaaatgccgt
     9121 ctgaaacggc ttcagacagc atccggtttc aaaaaccgtt cagaacaagc cgtgaatcac
     9181 gccgtgttcg tccacatcga ttttctcggc ggccggaact ttcggcagac ccggcatctt
     9241 catcatattg ccgcacagcg cgacgatgaa gcccgcgccg gcggaaacgg tgatgccgcg
     9301 cacggcgatg cggaagcctt cggggcagcc caacagtttg gcgttgtcac tcaaagagta
     9361 ttgggttttc gccatgcaga tcggcatttt gtccaagccc agtttttcca gtgaagcgat
     9421 ttcggcagac gcttccgcgc tgaaatcaac atcttccgcg ccgtacactt tttgggcaat
     9481 cgcacggatt ttgtctttga tgcccaactc gacatcgtag gcgaaaccga agttattggt
     9541 ttgactttca atggcgttga cgactttgcg cgccaaatcc gcgccgcccg caccaccttt
     9601 gccccacact tcggtcaggg aaacttcaac gccgtgttcg gcacaggctt tttcaatcat
     9661 cgccaactcg gcatcggagt cggacacgaa gcggttgagc gcaacgacga cgggcagtcc
     9721 gaatacgttt ttcaggttgg aaatgtgttt cagcaggttg ggcaaacctt tttccaaagc
     9781 gtctaaattt tcttcgccga ggttggcgcg ttccacgccg ccgttatatt tcaacgcgcg
     9841 gacagtcgcc acgacaacag ccgcatcagg tttcaaaccg gcaaggcggc atttgatgtc
     9901 gcagaatttt tccgcgccca agtccgcgcc gaagcctgct tcggttacgg cgtaatcggc
     9961 aaggtgtttc gccagacggg ttgcggttac ggagttgcag ccgtgggcga tgttggcgaa
    10021 cgggccgccg tgtacgaagg cgggcgtgcc ttcgatggtt tgcaccaagt taggcttaat
    10081 cgcatctttg agcagggccg ccatcgcgcc gtgtgccttc aaatctttgg cgtaaacggg
    10141 gctgccgtct ttggcgtagg cgacaaggat gttgcccaaa cgctctttca aatcgccgat
    10201 gtctttggcg agacagaata ccgccatcac ttcggaggca acggtaatgt cgaaaccgtc
    10261 ggggcgcatc acaccgtcaa caggtttacc catgccgtcg atgatgttgc gcaactggcg
    10321 gtcgttcata tcgaccacgc gccgccacag cacgcgtttg gggtcgatgt tcaactcgtt
    10381 gccttggtag atatggttgt cgagcatggc agcgagcagg ttattcgccg caccgatggc
    10441 gtgaaagtcg ccggtgaagt gcaggttgat gtcttccatc ggcaaaactt gggcatagcc
    10501 gccgcctgcc gcgccgcctt tcacgccgaa caccggcccc agagaaggtt cgcgcagggc
    10561 aatcacggca tctttgccga tgtggcgcaa cgcgtccgcc aaaccgatgg ttacggtggt
    10621 tttgccttcg cccgccggag tcgggttgat ggcggtaacc aaaatcagcc tgccctgttt
    10681 ttgcagcagt ttgaacgctt cggcaggatt gattttcgcc ttgtaatgac cgtaaggctc
    10741 aatgttgtcg gcgttcagac ccagcttggc ggcaatttcg ccaatcgggc gcatcgtgga
    10801 agactgggcg atttcggcat cggttttgaa gctcatgatt ttcctttaga aatgaggagg
    10861 gacatactgt ctgaaagcat caggcgacaa acaggtggat tgaaaataat atcaggcata
    10921 ttataacgtt atccgcacca aacccgcagt gaaatttttg acgctgcaac aaaaataccg
    10981 ttcatattgt tcacaatcca aggagaaaac atgggcagca acgcatggct gttttgggca
    11041 ttggcatcgg caggcttcgc ctcattgacc gccattttcg ccaaaatggg tttacagggc
    11101 atagattcgg atttcgccac ctttatccgc accttggtca tcctcgccgc tttggtattg
    11161 tttttaacct acaccggcaa atggcagggc gtgaacggct ttaccggcct caactggaca
    11221 ttcctcatcc tatccggcct cgctaccggc gcatcttggc tcgcctactt caaagccctg
    11281 caactgggta acgcctcgca agtcgccccc gtcgataaat tcagcctggt tctggtcgcc
    11341 ctgatggcag tagtcttcct caacgaacgc cccagcacgc aggaatggat aggcttgggg
    11401 ctggtaacgg cgggcgtgtt ggtgctggcg ttgaaacgtt aaaccgaatc cgccataccg
    11461 cctgaaaccg ggttttgaca atctgacggg cggcggtctg aacaaagtta cccaataaaa
    11521 tccgacaccg cgccgacggc aaaccgccgc atccgacagg ggtcggcttc agatggcatt
    11581 tgttggacgg aaagccccga acgccctatt ttcacgcctc cacgaaaata tgctggacat
    11641 ccggtcggaa ccgttcggca gccggctcgg caagccaccg ttttatttca cttccaagcc
    11701 cctgcaaggg cttgagcctc tttcagacgg cataccgtgc cgacatccag ccacaagccc
    11761 gtatgcttct gaccgctcac gcggttttgc cgcatttcgc cacgcaatac gggcgcgagt
    11821 ttcgccacac tgcccgcttc gattccgtca aacatttcag gacggtaaat acccacgccg
    11881 ctgaatgtca atccgttgcc gccatttact tccggccgca cgctgccgtc gggcagcagg
    11941 gaaaaatcgc cgtcggggtt gtgcggcgga ttttccacca gccacagatg ggcggaaata
    12001 tgttccggca gggacgatgc cgcctgaaac gcggcggtaa aatcgatgtc ggtcagcacg
    12061 tcgccgttga ccaccaaaaa cggctgccca cccaacagcg gcaatgcctg cgcgatgccg
    12121 cctgccgttt ccaaaccgcc tgcgggttcg ggcgaatagg cgatgttcac gccataagcc
    12181 gagccgtcgc ccaaagcatc ttctatctgc cgacccagcc aagcgtggtt gatgacgatt
    12241 tcggtaaacc ccgcctgctt cagacggcat aggtgccaac cgattagagg cttacccgcc
    12301 acatcgagca gcggcttcgg agtggtatcg gtcaaagggc gcatacgctc gccgcgtcct
    12361 gccgccagta tcatcgcttt catatatctg tccgaatatc agtctaaaaa tctaaactgc
    12421 cgtctgaaat acagcagcgc ggggcgtttg cacccgcagt ttttgatttc gtcgagcctg
    12481 acgtaaaaca caaaatgcgt gccgatttca tgtttgccga caatatgccc gtgcaggtgc
    12541 gccaacgcgc cttctatttc aggttgtccg gttttaccgt aatgccaaat atgataggca
    12601 aaacgttctt cgggtgaaag attggtcaat ccggcaaaat gttcggccac atcctgatgt
    12661 tcgtctgcca acgtattgat gcacaggctg ccgttttccg acagtatcgg gatgatacgg
    12721 gctttacggt tgatgcacag catcacggtc ggcggctcgt ccgttaccgg cgcgaccgcc
    12781 gtcattgtaa tgccgtaacg ccctgccgca ccgtctgtcg tgatgacatg aacgcctgcc
    12841 gcacaggatg ccatcgcatc acggaacgaa gtttgaaaag ttttctgcaa atccgccatt
    12901 tttccccttt aaactgtccc ctatataaga atgctgcaca caaggcaccc cccatgtgca
    12961 gcagttctga ttcaaaaagc cgtcggtcgg acatttccgc gcgttacggc gtattacgag
    13021 ttcaacgcat cctcgatttt ggcaagttct gccaacaggt ctttaagcag cagcattttc
    13081 tcgcggccca gcacttcctc gatagcgtcg taacgctcgt ccacttcttc gccgatttcc
    13141 tcatacagct tctcgccctc ggcagtcagc ttcagaaaaa cacgtcgttg gtcgttggaa
    13201 ggtttcaggc ggacaaccaa acccgctttt tcaaggcggg tcaggatacc ggtcaggctg
    13261 gggcgcagaa tacacgcctg attcgccaaa tcttgaaagt ccagcgtgcc gttttccgcc
    13321 aaaagacgga taatccgcca ttgctgatcg gtaatattcg cctgattcag aataggcctg
    13381 aattgggtca tcagggcttc ccttgcctgt atcagactga tattgataga cgcatgtttt
    13441 gattgggtag gcattgttca agtctccaag ttatcgaaaa tcaaactttc aaaccgtcgg
    13501 gaaagcctgt gggcgtaatt tttgatgcaa ccgttatata acaaaacgaa catatagcaa
    13561 caatacgcta taaaccgcat cggacgactg ggtataaaag actttaattc cgataatcct
    13621 atctaaaaat attttaatag ttatatctta gtctattttc cctacaatct caacaaagta
    13681 ttacatttac tgtcgaaaga actttcttcc agaaaaaaca aaagccgccg catcagacga
    13741 gcaaggtata tgccgtttga ttctccacat agcgtaaagt ttaacaaaaa caaaagttaa
    13801 ccgaaaacat ccgcctgaaa aattcgtgcg cgcaagcccc aataactgct gattcccgtc
    13861 gtatagtgaa ccattttccc atttttgacc aaaacgacgg caggcgttgc gacaatccgc
    13921 caagaccttg ccaaaccccc gtcctcatcg ttgaccgtcg gaaagcccaa gccccgcttt
    13981 gccatatacg ccgccacttc ggacgcgctg ccggaacgta ccgccacgcc gacgaccgga
    14041 acgccgtccg ccgccaaatc atcgattatc ggcgactgat aacggcacac gccgcaccag
    14101 ctcccccaaa aatacaccag caccgtccta tcccggctaa actgtcccaa agtcagccgc
    14161 tgccccgaca gcagggtcaa aggccgccct gccgcaccgg ccggctcttc gggcttgcgt
    14221 atccaatcca aaaacagcga caccaataaa aacaccaatg ccgtctgaac ggcaaatttg
    14281 atgcccgaaa gcagtttctt tttcatacgc tctctcaaac ggtacgccca cgcaaccggc
    14341 aggcaaacaa aaagccaagt ctcaaaactt ggcttccggt tatctggtgg gtcgtgagcg
    14401 attcgaacgc tcgaccaacg gattaaaagt ccgctgctct accgactgag ctaacgaccc
    14461 gataagccgt gcattataca gcaccatcct gcctcgtcaa gcaaatttta caggcttaat
    14521 tgcagaccac tgtttgcacg ggatattttg acaacggatt ttcacaatcc gccgcatacc
    14581 gtgtaaaagt tcgcacaagg aaaagcaaac cgcccgaaat caatgtacac tttccgctcg
    14641 tttcccttcc caaactgcac acagaaacac acattatgaa catacaaaac atccgcaccc
    14701 tcctcgacac cgtcgccgtt ccgaatacgg cacgcacgct cggcggcgaa aaggccgtcc
    14761 gttcggtcga acagcgttca gacggcatcc atatcgccct gcatttcggc ttccccgtcg
    14821 cgcacattgc ctcagaaaca gccgaccgca tacaagaaat cctgatgccc gaaacaggcg
    14881 acacacacat ccatctgtcc atagacaccg aaatcggcac acacaaagtc cagcccggcg
    14941 ttaccaccat caaaggcgtg aaaaacatca tcgccgtcgc atcgggaaaa ggcggcgtgg
    15001 gcaaatcgac gacgacagcc aaccttgccg ccgcaatggc gcggatgggc gcgcgcgtcg
    15061 gcgtgctcga tgccgacctt tacggcccga gccaaccgac catgttgggt gtggacgacc
    15121 gcaaacccga tcagaaaaac caaaaactca ttcccgtcga atcttcagac ggcatacagg
    15181 tcatgtctat cggctttctc gtcgataccg accaagccgt cgtctggcgc gggccgatgg
    15241 tcagccaagc cttgcagcag ctgatgttcc aaagcgagtg ggacgaagtg gactatcttt
    15301 tcatcgacct gcccccgggt acgggcgaca tccagctcac gctgtcccag cgcatccccg
    15361 taaccggttc cgtcatcgta accacgccgc aggacatcgc cctgatagac gcgcgcaaag
    15421 ccgtggatat gttccgcaag gtcaacattc ccattttagg cgtattggaa aatatgtccg
    15481 tccacatctg caccaactgc ggacacagcg aagccctgtt cggcacggac ggcggcaaag
    15541 atttagccgc acgcctcaac gtccccctgc tcggacagct tcccctaagc ctgcccgtgc
    15601 gcgaagccat ggacggcggc acaccggcgc aactgttcga cgaacacccc gccatcgcca
    15661 aaatctacac cgatgccgca ttccaaatcg ccctgggcat tgccgacaaa ggcaaagact
    15721 tcagcagccg cttccccaaa atcgtcgtcg aataaagccg cgtccgaaac cgcaacagca
    15781 atgccgtccc aagccccgcg cctgccggcg ggcttcatag tcctctctcc ccgtgggaga
    15841 gggctggaga gagggcaaca agccgcaagg cttgtattgg ggcggttaag ctgttgggaa
    15901 agattgccgc aacttggaga atgccctctc cccggccctc ccccacaggg gagggagcgg
    15961 attgcggcag gttttgcggt tgcaggcggt ttgaaaaaca gattggattt acaaccgttg
    16021 atgcaggtcg tctgaaaagc aaaaagcagc ctgcacaacc tgattagttt acaaaaccct
    16081 taaccccaac agcctccccg tcctctctcc ccgtgggaga gggctggaga gagggcaaca
    16141 agccgaaagg cttgtattgg ggcggttgag ttgttgggaa aggttgccgc aacttggaaa
    16201 atgccgcctc cccggccctc ccccacgggg gagggagtgg gtttcggcag atttttcggt
    16261 tgcaggcgat ttgaaaggca acttagattt gcagctgttg tttcaggtcg tctgaaaaat
    16321 aaaaagcagc ctgcacaacc tgttttcctt gcagcaaacc tcaaccccaa cagccgaccc
    16381 gtcctctctc cctgtgggag agagctagag agagggcaac aagccgaaag gcttgtattg
//
LOCUS       DAXHNE010000032        15941 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.032, whole genome shotgun sequence.
ACCESSION   DAXHNE010000032 DAXHNE010000000
VERSION     DAXHNE010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15941
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.032"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(60..135)
                     /locus_tag="ACFM4Z_000794"
     tRNA            complement(60..135)
                     /locus_tag="ACFM4Z_000794"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(99..101),aa:Glu,seq:ttc)
     gene            complement(855..2267)
                     /gene="trkA"
                     /locus_tag="ACFM4Z_000795"
     CDS             complement(855..2267)
                     /gene="trkA"
                     /locus_tag="ACFM4Z_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFC6020861.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(2365..3888)
                     /locus_tag="ACFM4Z_000796"
     CDS             complement(2365..3888)
                     /locus_tag="ACFM4Z_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HFC6020862.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMNVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGGKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPRKWQAKIGIIPVES"
     gene            complement(4195..5001)
                     /locus_tag="ACFM4Z_000797"
     CDS             complement(4195..5001)
                     /locus_tag="ACFM4Z_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC6020863.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDTAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKVSSSEDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPAKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            complement(5053..5304)
                     /locus_tag="ACFM4Z_000798"
     CDS             complement(5053..5304)
                     /locus_tag="ACFM4Z_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020864.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            5404..6165
                     /locus_tag="ACFM4Z_000799"
     CDS             5404..6165
                     /locus_tag="ACFM4Z_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020865.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRARVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRIRFDTKKDSVEFETGQIESGENADEAESGTNEPAVSENGENDAEETLMPV
                     QLNILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            6310..7479
                     /gene="metZ"
                     /locus_tag="ACFM4Z_000800"
     CDS             6310..7479
                     /gene="metZ"
                     /locus_tag="ACFM4Z_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HFC6020866.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VVSSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGAILVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAMYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAALIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            7565..7771
                     /locus_tag="ACFM4Z_000801"
     CDS             7565..7771
                     /locus_tag="ACFM4Z_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HFC6020867.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            complement(8715..9467)
                     /locus_tag="ACFM4Z_000802"
     CDS             complement(8715..9467)
                     /locus_tag="ACFM4Z_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6020868.1"
                     /translation="MTDILIDNTATEAVRALIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQDTAAHINLHFGNAAEQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
     gene            complement(9571..10038)
                     /locus_tag="ACFM4Z_000803"
     CDS             complement(9571..10038)
                     /locus_tag="ACFM4Z_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004518841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6020869.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            complement(10031..11607)
                     /locus_tag="ACFM4Z_000804"
                     /pseudo
     CDS             complement(10031..11607)
                     /locus_tag="ACFM4Z_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
     gene            complement(11628..13931)
                     /gene="parC"
                     /locus_tag="ACFM4Z_000805"
     CDS             complement(11628..13931)
                     /gene="parC"
                     /locus_tag="ACFM4Z_000805"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFC6020870.1"
                     /translation="MNTQAHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPPHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSLGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLADMVGRVKAGKVVMTTDSGETVLPPIAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESLTHTAVSSEPEILIESEGRRGAAHKDRIPV
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            14079..14762
                     /locus_tag="ACFM4Z_000806"
     CDS             14079..14762
                     /locus_tag="ACFM4Z_000806"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFC6020871.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGQLQGLDKKQTAE
                     KYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            complement(14835..15779)
                     /gene="tehA"
                     /locus_tag="ACFM4Z_000807"
     CDS             complement(14835..15779)
                     /gene="tehA"
                     /locus_tag="ACFM4Z_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFC6020872.1"
                     /translation="MSASKKFPIPLSYFSIALGLFALGLSWRYGASVGLLPALAAESL
                     LAAASVVWLLLVAAYLIKMFAYRNDFLSDLRDLVQCCFISAIPITAMLEGLALKPYQA
                     GAAAVLIYVGVAGQLAFSMYRAAGLWRGLHSLEATTPIIYLPTVATNFVSASSLAALG
                     HHDYAALFFGAGMFSWLSLEASILGRLRTAAPVGTAARGVVGIQLAPAFVGCGAYFAV
                     GGKVDGFALALIGYGCLQLLFLLRLTRWFWEGGFTMSFWGFSFGFAAMAGCGLHLAAS
                     GVLSGLGLTLATAGSAGVALLLVGTLHRIATGRFLVRS"
BASE COUNT         3468 a         4029 c         4651 g         3793 t
ORIGIN      
        1 aattgcggga atgacgactc ataataaaaa acctgcttga aacaagcagg tttttgaatt
       61 ggcacgccca cggggattcg aaccccggtt gccgccgtga aagggcagtg tcctaggcct
      121 ctagacgatg ggcgcgtgga aacggacatt atacatactt atagaaattt agcaatacga
      181 tgccgtctaa acaaatgtca ggggggcgtt tgtctgatcg catcacaata taaaaacata
      241 taaaaaacat tggaatatca agcaattcat ttttttgcgt ggtaatgccg acaacggaag
      301 gcagctttat tttgaggcgc aaaggtcttc gatgcacacg atgcgctaga cttggcaggt
      361 caatatgggc gtgcgcagta tgttctgatg cggcggattc gcctgaaaac aagccggaac
      421 ggtcagatgc gttccggatt gtttttttgt cgggggggtg ccgccatatc aaaacaggcg
      481 ggcgatgccg tctgaaactt cgtcattccc gcgaaagcgg gaatctagaa cttttaaatt
      541 ttcagacggc ttttgaatat tgccgttgcc cgacgttctg gattcccgca acttttcgtc
      601 attcccgcga aagcgggaat ctagaacctc taaattttca gataaccttt gaatattgcc
      661 gctatcccaa ggtctggatt cccgcctgcg cgggaatgac gggtctttta taacctttga
      721 atattgccgc tatcccaagg tctggatgcc cgcttacgcg ggaatgacgg ggagggcgga
      781 tgccgtctga agcccaccta ccattatttc cgcttccata ccgcttggcg gcaatatgtc
      841 cggaatgggg cggtttatcc gaaaaagccc attttgacct ggatgagttt ttccagttcg
      901 ttcaggatgc gccggcgcga gacgaaaaag atgatgtggt cgccgtcttg gatgacggtt
      961 tcggtatggt gtcccataat ggtttcgccg gttccggcgc ggacgacggc ggcaatgtgg
     1021 cagccttcgg gccatttgat gccgctgatg cgcctgccga tgatggcgga agtttttttg
     1081 tcgccgtgtg cgacgacttc gatggcttcc gccgtgccgc gccggatggg gtggacggca
     1141 acgatgtcgc cgcgccggat gtgggcgagt atcgagccga tggtgatgag gtggggggag
     1201 acgacgatgt cgattttgtt gccttcgagc aaatcgacgt agcttgagcg gttgacgatg
     1261 ccgatgacgc gcttcgcgcc gaggtttttc gccaaaaggg cggacataat gttgctttcg
     1321 tcgtcgttgg tcagggcgca gaatacgtcg atttcgtcga tgtattcgtt gtcgagcagg
     1381 gtttcgtcgg ttgccgaacc ttgcaggacg agggtgttgt cgaggttttc ggctatccat
     1441 tcggcacggc gcggccggca ttcgatgatt ttgacgttgt atgcgtgttc gagctgcttg
     1501 gcgagacggt agccgatgtt gccgccgccg gcaatcatga tgcggcgggt gctggtttct
     1561 ttggggcgca attcgggtat gaccgcgccg atgttttcgg cggcggcggc aaataggatt
     1621 tcgtcgcctt cgatgatgac ggtttgcggc gcggggacga tgaggcggtt gttgcggtaa
     1681 acggcgcaga tttggcagtc ggccccgtcg ggcaaatctt gggcgatgtc ggcaatgctg
     1741 cgtccgacaa gcagtccgcc gcgccgcgcc tgtatgatga ccatccgcac gcggtcgtct
     1801 gcaaaacgta aaacctgcaa tgcgcccggg cagtctatca ggccggcaag ctgttcggta
     1861 acgagctgtt cggggctgat ggtttcggtt atgccgaata tggaaaggct gccgttttcg
     1921 ttgttttcga gcttggggct gaggtattcg aggtattcgc tggaacggac gcgcgcgatg
     1981 cggccgggga tgttgaacag gtcggcggca actttgcagg cgacgatgtt ggtttcgtcg
     2041 ctgcgggaga gcgcgagcag caagtcggca tcttccgcgc cggcgcgttc taatgtgaag
     2101 ggggatgcgc cgttgccgaa aacggtttgg acatcgaggc ggctgcctgt ttcctgcaat
     2161 gctttttcgt cgatgtcgat aacggttacg tcgttgttgg gtatggcggc aaggttttgt
     2221 gcgacggtag aacctacctg tccgttgcct aaaatgagga ttttcacggt gtgggtcgcc
     2281 gagtgatgaa atggtggatt gtaccgcaac cgggggcgga aatgccgtct gaaggcagat
     2341 tttgtgttca gacggcatgg cgcctcaaga ttcgacgggg atgatgccga ttttcgcctg
     2401 ccatttgcgc ggggcggtgg cgtggatgga ttcgcctttg ctatctacgg cgacggttac
     2461 gggcatgtct ttgacttcaa attcgtaaat ggcttccatg cccaattcgg ggaacgccaa
     2521 gactttggaa gatttgatgg cttttgccac gagatacgcc gcgccgccga ctgccatgag
     2581 gtacacggct ttgttgtcgg cgatggcttc gcaggtggcc acgccgcgct cggatttgcc
     2641 gatcatgccc aagaggtcgg tttgttcgag catttggcgg gtgaatttgt ccatgcgggt
     2701 ggctgtggtc ggacctgccg gaccgacgac ttcatcgccg accggatcga cggggccgac
     2761 gtagtaaatc aggcggttgg tgaaatcgac gggcaattct tcgcctttgt tgagcatatc
     2821 gacgaggcgt ttgtgtgcgg catcgcggcc ggtgaggatt ttgccgttca acagcaatac
     2881 gtcgccggtt ttccagcttg ccacttcttc tttggtcagc ttgtcgacat cgacgcgttt
     2941 gccgttgtcg gggctgtaag tcaaatcggg ccagtcttcg acgcgcggcg gcgtgagttc
     3001 gacagggcct gagccgtcca attcaaattc gacgtggcgg gtggcggcgc agttcggaat
     3061 catggcaatc ggtttggagg cggcgtgggt cggataatcg aggattttca cgtccaacac
     3121 ggtggtcagt ccgcccaagc cttgtgcgcc gatgcccagc gcgttgactt tttcaaagag
     3181 ttcgaggcgc agggcttcgg tggtggacaa ttccgcgccg gacgcggcct tttcctgcaa
     3241 ttcttgaatg tcgatgtggc tcatcaggga ctcttttgcc atcagcacgg ctttttcggg
     3301 cgtgccgccg atgccgatac ccaagatgcc gggaggacac cagcccgcgc ccatggtcgg
     3361 gatggttttc aatacccaat cgacgatgtt gtcggaagga ttgagcatgg cgagtttgga
     3421 tttgttttca gagccgccgc cttttgccgc gcaggttact tcgactttac cgcccggcac
     3481 gatgctcata tggatgacgg cgggggtgtt gtctttggtg ttttggcgtt tgccggccgg
     3541 atcggcgagg acggaagcgc gcagcgtatt gccttcccaa gtgtaggcgc ggcgtacgcc
     3601 ttcgttaacc atctcttcca cgctcatgtc cgcatcccat tggacgttca taccgacttt
     3661 gaggaagacg gttgcgatac ctgtgtcttg gcagatgggg cggttgtttt ccgcacacat
     3721 acggctgttg accaaaatct gcgtcatcgc gtctttggcg gcaggatttt cttccttctg
     3781 ccacgcctta taaagcgcgt cgatgtagtc tttgggatga tagtagctga tgaattggaa
     3841 ggcatcgcaa atgctttgga taaagtcttc ctgtttgatg acggtcatga ttgtgttcct
     3901 tttcaaggtt gtggcggtaa gtgtctgctg ctgttttaaa caaccttttt gaaggctgtt
     3961 ttttatcagg ttgaaatagt gccagcgttt gtacggtaaa gacaatacat aattctaatc
     4021 tcttgattat caatttgttt taataaaata ctgttttact cggattttga tatttgtaat
     4081 ctttgcctgt tgctttgcgg tagggagaaa tcagcaggca gttcattgta ggccgattga
     4141 tgaacaatgc cgtctgaaag ggcttcagac ggcattgtgt tcggacgggc gggcttattt
     4201 tgcggcctgt ccgtcttctt ttgcaaaata tttggcgtaa atcttgtcgt attcgccgct
     4261 ttcgcgtact ttttccaacg catcgttcag cattttgacg gttgcttcgt cgcctttgcg
     4321 taccgcgatg ccgtagtgtt cggtggtgaa gtcgggcagg gtaacgaagt ccatcccttt
     4381 ggccggattg tttttcacat aattggcgat gaccgcgctg tcgctgacca cggaatccaa
     4441 gccgccgttt tccagttctt tgataatcag gggaacgttt tcaaagcgcg cgattttcgg
     4501 attgtcgttg cccaagagtt tggatacgga gaaatcgccc gtgtagccgg ttaccacgcc
     4561 gactttgttc atgtttttca aatcttcgga agaagatact tttttgcctt tcggaacgag
     4621 gacgacttgg gtgatttcaa aatacgggtc gctgaagtcc atagactgtt tgcggtcgtc
     4681 ggtaatggtt acgcccgaca tcacaacgtc cgcatcgccg ttgtttaagg cggggaaaag
     4741 gctgtcccac ggctggtgtt tgaattcgat tttaaaattg cccgccttcg ccatcgcgtt
     4801 catcaaatcc acatcgaaac cttcgacatt gcctttcgag tctaaagatt caaagggggc
     4861 aaactcggcg ttggaagcca cgcggtacac tttgtcgggg ttggcggcag gcgcggcggt
     4921 atctttgccc tgaccgccgc aggcagacag cgcgagcgcg gaacaggcaa gggcggcggc
     4981 aatccatttt ttcatattca tcaaaattcc tttcggtaat caaaaagcct aaaaaatcag
     5041 tttaacataa aattaacgtt ctttttcacg gaaaaacgca taagtaacgt cgtggaacaa
     5101 atccgtatgc gggctgtcct catagctcaa ttctgccaag gcgtcctcaa aagcaagctg
     5161 aatcgactcg accgcgattt cggcggtttc ggcgggaagg gcgcggatta aggctttgaa
     5221 tgcggtggac aggacgtggt tctgcatacg cagcacgtcg ttcgcctctt ccagatattc
     5281 caaacgctgt tcgatgctgc ccatgatgta tcctcgcgaa tctgttaaaa tgcgccatta
     5341 tatcaaaccg cgctttgggc gcgggtttgt ctgcttgcaa agattaagaa ccggaagccg
     5401 aaaatggatg cgaataaaga gccgaattgg gaaaaacgtt tggaaaacct gcgtcagacg
     5461 cgccgtatga aggcagtgcc gacttacgcg ccgctgtccc gcccctctga aaccggaacg
     5521 gacgcgcctg tgccgaaact ggacgatgag agccgagccc gtgtgttgaa ggcgtatctg
     5581 aaaaaatggc aggaggaaca caatgccgcc gttgccgatt gcggcgagga agtcgaaacc
     5641 gacccgatga tcgtccttca ggaaaactgg ctcaacgcgc aggcttccct gcaaatgtcg
     5701 gtttcggaaa aacacatcga atcgcggcgg agaatccggt ttgataccaa aaaagacagt
     5761 gtcgaatttg aaaccggaca gattgaaagc ggggaaaacg ccgacgaggc agaatccggc
     5821 acgaatgagc cggcggtttc ggaaaacggg gaaaacgatg cggaagaaac gttgatgccc
     5881 gtccaactca atatcctgaa cccgaaagcc gtcaaccggc gcgaagtgtt ctgcctgtcc
     5941 gaacaggagc tgaccgaacg gctgatcaaa cgcctgcgcc cgcatctgac cgataccgtc
     6001 aacggtatca tccgtacggc ggtgcaaaag cagatggcgg tttttaccta tcagctccag
     6061 cagatgctgc acgagcaggc gggtgcggta gtcgaagacg tgttggaaca cgatgtccgc
     6121 aaaatcctca acgacatcaa gtacgagtta aaatacaaac gctgattgcc atgccgtctg
     6181 aaagccgttt cagacggcat ttgcattctc acccgacttg aaagcatcat agaacaggcg
     6241 caaaatatta acaaattaaa caacgggcgc acgccccgtt tttgaacgga caaaaccgag
     6301 gaatcaccga tgagcaaaaa actccatccg caaacgctcg ccatacgcgg cggcaaagaa
     6361 caaaccggat accgcgaaca caatcaggca ttgttcctga ccagcagttt catgtgggac
     6421 aacgcccaac acgctgccga tttgttttca aaaaaaatca aaggcttcac ttatacccgt
     6481 accgccaacc cgaccaccgc cgcctttgaa aaacgcatcg ccgctttgga aggtgcggaa
     6541 cgcgcggtcg ccacttcgac cggcatgtct gcgattcagg cggcgttttt cacctttttg
     6601 caggcgggcg accacgtggt ttccagccgc agcctgttcg gcacgaccgt cggctttatc
     6661 aataacatcg ttaccaaatt cggcatcgaa gtgagccacg tgtcgccgac tgatataaac
     6721 gaatggaaag ccgccgtcaa agccaacacc aaactgctgt ttttggaaac gccgtccaat
     6781 cccttgggcg aagtggccga cttggaagcc ttggcggaat tggcgcacgg catcggcgcg
     6841 attttggtgg tggacaacag cctgctgtcg cccgtcggct cgcagccttt gaaacacggc
     6901 gcggatattt ccgtttcctc cgcgaccaaa gccatagacg gacacgggcg cgtgatgggc
     6961 ggcgtgttgg cgggttcgga agagctgatg gcgcaggtcg ctatgtattg caactcttgc
     7021 gggctggcaa tgtcgccgtt taatgcgtgg cagttgttga gcggcgtgga aaccctgtcg
     7081 ctgcgtatgg aaaaacagtt cgacaacgcc ctgaaaatcg cgcaatggct gcaagcgcag
     7141 ccgcaggttc aagccgtgta ctacaccgga ttgcccgacc atccgcaggc ggcgcttatc
     7201 cgcaaacagc aaaacggcgg cggcatcgtc atcggctttg aagttgccga ccaagcagcc
     7261 gcgtggaaag tcgtggacgg cgtggaactc ttttcccgta ccgccaacct cggcgacgtg
     7321 cgctcgacca tcacccatcc gtggacaacc acgcacggca ggatgcagcc cgaagaaaaa
     7381 ctcgccgcca acatccgccc cggattggtg cgcctgtcgg tcggtttgga atatgttggc
     7441 gatttgattg acgatttgaa acaggcactc gcgcgctgat tgcgtgtttc agacggcatt
     7501 ggacagcaga tgccgtctga aacccttatc gcgtatggca tacacatcaa caataggaaa
     7561 aaatatggat gaagcagttt ttgccgattg ggcgttgaaa atctgcctga ccggcttgat
     7621 tatttttttg ggttttatcg tttggaattt gggcaaggag tccaaagcag gcaaattcgg
     7681 catcgccatt ttgtttttgg ttttgggctt gggcgtgttc ggttttgtgt tcaaagagtt
     7741 attgattaag tttttggtat tgccgaaata ggtttgcggt ctgtcaaaat gctgtctgaa
     7801 atcataaaaa gtaatctgca ccctaaaagt ttgatttaac cgccaactga ttaaggtgcg
     7861 gatttttatg acaaaataaa aatcatcttt ccgcagtcat tcccacggac gcgaaagtct
     7921 gtctttttga atcccggata attttacatc gtcattcccg cgcaggcggg aatccagatc
     7981 attgggtagc ggcaatgttc aaaagtcgtc tgaaaaatca gaagttctag attcccgttt
     8041 ttacgggaat gacggaattt cagacggcat cctcccaccc cgtcattccc gcgcaggcgg
     8101 gaatctagtc cgttcggttt cggtcattta cgataaattc ctgttgcttt tcatttctgg
     8161 attcccactt ccgtgggaat gacggaggag cggataaatt ctcgcaatct aaaaccttgt
     8221 catttccata aaaaacagca acccgaaaca gcaacttaaa aacagcaacc tgaaacctcg
     8281 tcattcccgc gcaggtggga atccagatca ttgggtggcg gcaatgttca aaagtcgtct
     8341 gaaaaatcag aagttctaga ttcccgtttt cacgggaatg acggaatttc agacggcatc
     8401 tgcccgcccc gtcattcccg cgtaggcggg aatctagtcc gttcggtttc agtcatttac
     8461 gataaattcc tgttgccttt catttctaga ttcccacttc cgtgggaatg acggtttgga
     8521 agttgcctga aactcgaaaa caactatagt ggtttaaatt taaaccacta taaaactgaa
     8581 tgaatcggat tcccacttct gtgggaatga caaactttaa ggtgtgatga cttatccaaa
     8641 acagtcgaaa cgcaaaaacc ggtttctcgt ttgcataaga acggcaaatt tttcggtgtc
     8701 ttgttttatg ggcgttatcc cttgtccgtc ccgtagggca ggtaaacgtc gaagcgggtg
     8761 cttttgccgg tgtattggta ggcaggggct tgtccggcaa ggtgttcgcc gaggcggggg
     8821 cgtttgacga cgacgcgttt ttttgctgtt tggcgtgcgg tatgcaggag tgccgcttca
     8881 tcttgcgctt cgccgacgag ccgatggaag taggtcattt cttttttaac ggcggcactt
     8941 ttgcggcgtt cgggatacat ggggtcgaga tagacgatgt cgggtttgcc ttgtgtttga
     9001 acaagggcgg gcatttgttc ggcggcgttg ccgaaatgga ggttgatgtg cgcggcggtg
     9061 tcttgcgttt cgggattgag gagggcgcgg cggatgccgt ctgaaagcag gcaggcgacg
     9121 gcgggatgtt gctcgaaggc ggtaacggcc agcccgagcg aggcgaggac gaagctgtcg
     9181 cgccccaatc ctgcggttgc gtcccaaacg gtggggtgcg cggtgtggtt gacggctttg
     9241 gcgatgagtt cgcccccgcc ttttgtgcgc cggtattgtg ccgcgccgga ggcaaaatcg
     9301 acgatgacgc tgcttttttc cccgacaagc ctgaggctga cggtatcgtg ttcggcaagg
     9361 aggtaactgc cttgttcggg cggttgggaa acgggcacaa gggggaatgc ccgtatcagg
     9421 gcgcggacgg cttcggtggc ggtgttgtca ataaggatgt cggtcatggt gttttgtgtc
     9481 cgttcgggcg tggggcgaac ccgctttccc gtgccgtttc agacggcatt ttttcgggtg
     9541 ggcggagatg gtgcggatgc cgttttgtcg tcagccgatg ttgaggcgtt ccatacggta
     9601 gcgcatggaa cggaagctga tgcccaagcg tttggcggcc tgcgtgcggt tgccttcggt
     9661 ttgtttgagg gtttgttcga tgatgtcgcg ttcgatttgg tcgagatagt cctgtatctg
     9721 catggtatcg gggtcgaacg cgccgcaacg cagggcttcc tgtgcctgat cggcattgcc
     9781 gaaggcggtg atgacggcaa ccggcgtatc gagcaggcgg ctgttgatgt gttggacgac
     9841 ttcgagcccc gagccgtccg gcatacgcat atcggtcagg acgagcgaat agcgttggct
     9901 gtcgagcttg tttttggctt cggcaacgcc tgacgcggta tggacgcgca agcccatttt
     9961 catcagggtc atttccatca ggtcgagaat gtcggtttcg tcatcgacga cgaggacggg
    10021 gtgttgcagt tcagtcattg gttttttccg gtaatgtgag ttcgaaacat ttggcttccg
    10081 gtaggtaggt caaatcgccg aaattggcgt gcgccagttc gcgggcgaca tacagcccga
    10141 ggccggtgcc gttttccgcc gtggtgtaaa agggttcgaa caggtgttcc tgcacttcgg
    10201 gcggtcggca aagaggatac agacggtgtt tttttgcgcg gggcggatgg tgacggaaat
    10261 cgagccgggc tgtttgcggc tgtgccgcca cgcgttgttg gcgaggttcc acataatttg
    10321 ccgcaggtgg gcaggatcga aataggcagt caggtgattg ccctgcatat ctaggcggat
    10381 gcagccggcg gcatcgggat ggccgagcag gaactcttgt ttgaattctt cccaaaacgg
    10441 tatcaggccg atggtttcgc gttcggtttt gttgcgcttg ttgagcgagg aaatatcttc
    10501 gagcattttg tcgatacggc agacgttgcc gtcgatgatt ttgcacaatt tggcgttgaa
    10561 cggatctgcc gcccccgctt ccatattttc gcgcaacagg tcgtttgcat ggcggatggc
    10621 ggacatgggg ttgcggattt cgtgggcgat gttggcggtc agttgtccga gcgcggcaag
    10681 tttgacggac agggcttctg cctgaatttc gctttgcggg cggatgtaga ggatgagcag
    10741 cttgttctgc tctttgttca tcggcacggc gcggatgcgg gcggtcaggg cgggcgtgtc
    10801 gatattgcgt tcgaaagtac gcgaaggggc cttgtcccat aaaacggtaa tagggtcgaa
    10861 cagggcggta tgctgtccga tttcaagcgt ggggagcaga tcttttgcct ttttattgaa
    10921 cagtacggtc tgatactcga cgttgatgat gacgaccgct tcctgaacgc ggttgagcac
    10981 gatttggttc aagcccctga tgcggcggta ggcggcgtgg ttttcatggg caagtttgcc
    11041 ggcacggtcg atgtatctga ccgacagcga ggcaatcatg gcgacgaaat aggagccggt
    11101 cacgaggacg aaggtgttgg tgacggtttt tgcatccaaa atcagcggat acatattgat
    11161 attgctgtct gcaagggcat tcaatatcag caggatggcg gcgtagctgg catagagcag
    11221 ggggtaacgc ccgtaactga gcaggcagga gctgcccacg aagggcagga tcaggatgcc
    11281 gaaccccgag ttgatgccgc cgaacaggta ggtcagtatg ccaatcatgg taatgtcgga
    11341 cacggcgctg aaactcggta tcttcaaagc ctgccattgc caatgcggat ggatgatgga
    11401 aaagaaaatc agccagcagg caatggcgag ccaaacataa aacgcccacg ccgtccacga
    11461 agcgtgcagg ttcgcacggc tgccggtttc cagcccgagg atgtgcataa tcatcagcgg
    11521 aaaaacgatg gcgacgcgga tgatgttgat cagattggga atccggtctt tgagtttttc
    11581 aagttcgcgg gggttggaaa tcaccataat tatgtgtgga atcgggttta tttgggggaa
    11641 gaaagctgtt tcaggctgcc cgatatgggt aatagtttgc cttttttgcc gcgttttgcc
    11701 tcaagcaggg agacggggat gcggtctttg tgcgccgcgc cgcgcctgcc ttcgctttca
    11761 atcaggattt ccggctcgga agaaacggcg gtatgcgtca gcgattcgcc ggcgtttaat
    11821 ccgatgattt gcagcccttt gcctttcgcc ataattttca attcgccgat ggggaaggcg
    11881 agggcgcggt tttgactggt ggcggcaatg attttgcagt cggggttgat gaacgaggag
    11941 gcatagacgg caatcggcgg caggacggtt tcgccactgt ctgtggtcat caccactttg
    12001 cccgctttta cgcgtccgac catatcagcc aacttggcga tgaagccgta gccgccgctg
    12061 cttgataata aataatgttg ttccggcaat cctgtcaaca tcgcaacggg tttcgcgccg
    12121 ttttgcaact cgatcaagga tgaaaccggc acgccgtcgc cgcgtccgcc gggaatttcg
    12181 gcggcatcga gtgtataggt tctgcccaat gaatcgagga tgacgacggg taaaaccgtt
    12241 ctgccttcaa gggtttgttt gaggcggtcg ccttctttga acgcggtttg gctcaaatcg
    12301 agattgtgtc cggcacggct gcgtatccag cctttttccg acagaatcag tgtgatgggt
    12361 tcgtcggcgg tggtttgtgt cagcacggcg cgtccggcct cttccaccag cgtgcggcgt
    12421 gcatcgccgt attgtttcat atccgcctgc atctctttga taatcagctt gcgcttttcg
    12481 ttttcatcgc ccaaaaggat gttcagacgg ccttgttctt cgcgcaattc gttcaattct
    12541 ttttcgagtt tgaaaccttc caaacgtgcc aactggcgca ggcggatttc caaaatgtct
    12601 tcggcttgga tttcggtcag cccgaacgcc gccatcaaat cggctttcgg gtcatctgat
    12661 tcgcggatga ctttaatcac ttcgtcgatg tgcagaaaga ctttcagacg gccttcgagg
    12721 atgtgcagcc gtttttccac ttggttcaaa cggaatttca gacggcgtgt tacggttacg
    12781 gtgcggaaat ccagccattc ctgcaaaatc gttttcaggt ttttctgcgc ggggcggttg
    12841 tccagcccca tcatcaccaa gttcatggac acattgcctt ccagcgaagt ttgcgccatc
    12901 agcgtgttga tgaaggtatc ggtatcgatg cggctggatt tcggttcgaa caccagtcgc
    12961 acgggatgtt cgccgtcgga ctcgtcgcgc acgcggtcga ttaaatccag catcagcttt
    13021 ttggtattga gctggtcttg gttgagctgt ttcttacccg ctttcggttt cgggttggtt
    13081 tgctcttcga tttcggcaag gattttggcg gaattggcgt tcggcggcag ctcggttacg
    13141 atgacgcgcc actgtccgcg cgccaatttt tcgatttcat aacgcgcacg cacgcgcacg
    13201 ctgcccttgc cggtttcata aatccggcgc aattcgtccg ccggcgtgat gatttgaccg
    13261 ccgccggcaa aatcgggggc aggaatatat tgcatcaggt cggcggtttc cagcgtcggc
    13321 tttttcaaca acgcaatcgc cgcctgcgtc acttcgttca aattgtgcgg cggaatctcg
    13381 gtcgccatgc ccaccgcaat gcctgacgcg ccgttgagca acaccatagg caggcgggcg
    13441 ggcaggtgca gcggttcgtc aaacgcgccg tcgtagttcg gcacaaaatc caccgtcccc
    13501 tgattgattt cggacaacag caattccgca atcggcgtca gccgcgcttc ggtgtaacgc
    13561 atcgccgccg ccccgtcgcc gtcgcgcgag ccgaagttgc cgatgccgtc gattaaggga
    13621 tagcgcaagg taaaatcctg cgccatccgc accatcgcct cataggcgga actgtcgccg
    13681 tgcgggtggt atttacccaa aatctcgccg accacgcgcg ccgatttcac cggcttcgcc
    13741 cccgccgtca aacccatatc gcgcatggca aacaaaatgc gccgctgcac gggcttctgg
    13801 ccgtctgaaa cttcaggcag cgcgcggcct ttgaccacgc tcatggcgta ttcgagatag
    13861 gcgcgttcgg cgtatcggcc gagcatcagc gtattggaat cggtatgtgg ggcgtgcgct
    13921 tgcgtattca ttgtgtatgc ggaatgtaaa gcaaaggaca ttattgtaaa tcaaaacggc
    13981 aaaccatagt gggcgcgtgt ctgaatctga atcaaatacg ggaaatgtga aaatatgtta
    14041 taaataaggc tttcccactt tcacacattg gagacgatat ggaactggta tttatccgcc
    14101 acggacaaag cgaatggaac gcgaaaaacc tgtttacagg ctggcgcgac gtgaagctga
    14161 gcgagcaggg gcttgccgag gctgccgccg ccggcaagaa actgaaagaa aacggctatg
    14221 agttcgacat cgccttcaca tccgtcctga cccgcgcgat taagacctgc aacatcgttt
    14281 tggaagaatc cgaccaactg ttcgtaccgc aaatcaaaac gtggcggctg aacgaacgcc
    14341 actacggcca actgcaaggc ctggacaaaa aacaaaccgc cgaaaaatac ggcgacgagc
    14401 aagtccgcat ctggcggcgc agctacgaca ccctgccgcc gcttttggac aaagacgatg
    14461 agttttccgc acacaaagac cgccgctatg cccatctgcc tgccgatgtc gtacccgacg
    14521 gcgaaaacct gaaagtaacg ctggagcgcg tattgccgtt ttgggaagac caaatcgccc
    14581 ccgcgatttt gagcggcaaa cgcgtcttgg tggcggcgca cggcaactcc ctgcgcgcgc
    14641 tggcaaaaca catcgagggc atttccgacg aagacattat gggcttggaa atcccgaccg
    14701 gtcagccgtt ggtgtacaaa ttggacgaca atttgaaagt catcgaaaaa ttctacctgt
    14761 aagggtgtga aataaaaaat gccgtctgaa tcccagcgtt tcagacggca tttttgacaa
    14821 ggcggcaaaa cgcatcagct gcgtaccaag aaacgccccg tcgctatccg gtgcagcgta
    14881 ccgacaagca gcagcgccac gcctgccgat ccggcggtgg caagcgtcag ccccaagccc
    14941 gacaatacgc cggaagccgc cagatgcaga ccgcatcctg ccattgccgc gaaaccgaat
    15001 gaaaatcccc aaaagctcat cgtaaaacca ccttcccaaa accagcgggt caggcgcagc
    15061 aagaacaaaa gctgcaggca gccgtagccg attaatgcca acgcaaaacc gtcgacttta
    15121 ccgccgacgg caaaatacgc gccgcagccg acaaaggcgg gcgcaagctg gatgccgacc
    15181 acgccgcgcg ccgccgtgcc gaccggtgcc gccgtgcgca gcctgcccaa gatggaggct
    15241 tccaagctca gccaggaaaa catacccgcg ccgaaaaaca aagctgcata atcatgatgc
    15301 cccaacgccg ccagagatga cgcgctgaca aagtttgtcg caaccgtagg cagataaata
    15361 atcggcgtcg tcgcctccaa ggaatgcagg ccgcgccaca gaccggccgc ccgatacatc
    15421 gaaaaagcca actgtccggc aacgccgaca taaatcagga ctgccgccgc gcctgcctga
    15481 tagggcttca gcgcgagtcc ctccagcata gcggtaatcg gaatcgcgct gatgaagcag
    15541 cattgcacca agtcgcgtaa atcagacaaa aaatcgtttc ggtacgcaaa cattttgatc
    15601 aggtatgccg ccaccagcaa gagccagacg accgaagccg ccgcaagcag cgattcggcg
    15661 gccaaggcgg gcagcagccc gacagacgcg ccgtaacgcc acgacagccc caaggcaaac
    15721 aagcccagcg cgatgctgaa atagctcaaa ggaatcggaa atttcttaga tgcagacata
    15781 aacacccttc agacggcatg ccgtcgtaac gcacagatat agtgaattaa caaaaaccag
    15841 tacgacgttg cctcgcctta gctcgaagag aacgattctc taaggtgctg aagcaccaag
    15901 tgaatcggtt ccgtactatc cgtactgtct gcggctcgcc g
//
LOCUS       DAXHNE010000033        15383 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.033, whole genome shotgun sequence.
ACCESSION   DAXHNE010000033 DAXHNE010000000
VERSION     DAXHNE010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15383
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.033"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(105..1889)
                     /gene="lpdA"
                     /locus_tag="ACFM4Z_000808"
     CDS             complement(105..1889)
                     /gene="lpdA"
                     /locus_tag="ACFM4Z_000808"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6020873.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGAAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAAPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMINTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRVPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1907..2344)
                     /locus_tag="ACFM4Z_000809"
     CDS             complement(1907..2344)
                     /locus_tag="ACFM4Z_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020874.1"
                     /translation="MGNFLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKA
                     THGPSVKNAVYAHQIETGLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQ
                     YSIFEYEVEHPENPNEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(2438..4045)
                     /gene="aceF"
                     /locus_tag="ACFM4Z_000810"
     CDS             complement(2438..4045)
                     /gene="aceF"
                     /locus_tag="ACFM4Z_000810"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFC6020875.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPAA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGSAIIEVETVGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAPS
                     APAAAKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIMGDDIKAFVKSVMQ
                     GGAAKPAAASASLGGGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPH
                     VTVHEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDN
                     LVLKNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQG
                     ACFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDH
                     RVIDGAAGMRFTVFLAKLLKDFRRITL"
     gene            complement(4195..6858)
                     /gene="aceE"
                     /locus_tag="ACFM4Z_000811"
     CDS             complement(4195..6858)
                     /gene="aceE"
                     /locus_tag="ACFM4Z_000811"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFC6020876.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDTQLKSSGDREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVVT
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSKLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALSA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7552..9264
                     /locus_tag="ACFM4Z_000812"
     CDS             7552..9264
                     /locus_tag="ACFM4Z_000812"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFC6020877.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERTAAQAELTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFAGKVYADFATEKGADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTKAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTA
                     SLNG"
     gene            9327..9983
                     /locus_tag="ACFM4Z_000813"
     CDS             9327..9983
                     /locus_tag="ACFM4Z_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFC6020878.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGTGEGKDILGCVAGYGVGLDLTA
                     RDIQCRLKEKGLPWLKAKGFRHSACVSDFAAAGRIGNPENVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9993..10448)
                     /gene="ruvX"
                     /locus_tag="ACFM4Z_000814"
     CDS             complement(9993..10448)
                     /gene="ruvX"
                     /locus_tag="ACFM4Z_000814"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFC6020879.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(10441..10989)
                     /locus_tag="ACFM4Z_000815"
     CDS             complement(10441..10989)
                     /locus_tag="ACFM4Z_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFC6020880.1"
                     /translation="MNLSNHFLIAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWSKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10991..11551)
                     /locus_tag="ACFM4Z_000816"
     CDS             complement(10991..11551)
                     /locus_tag="ACFM4Z_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HFC6020881.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNMLGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENLDKR"
     gene            11879..13684
                     /locus_tag="ACFM4Z_000817"
     CDS             11879..13684
                     /locus_tag="ACFM4Z_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC6020882.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLE
                     QKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRA
                     EMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGV
                     FYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAA
                     GSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13804..15288
                     /locus_tag="ACFM4Z_000818"
     CDS             13804..15288
                     /locus_tag="ACFM4Z_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFC6020883.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLNGAVGVSAAFLPPDALVVSTKGREG
                     TEGFALKARPEDYIFKAGTDPLAGIPAELKTIPMTVLINSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
BASE COUNT         3712 a         3764 c         4239 g         3668 t
ORIGIN      
        1 gtcggagaag atattttaat ccgctgtaaa atagaaaatg ccgtctgaac atttcagacg
       61 gcattttctc aaataaacct tatcggctgt ttattcagtc ggatttattt tttcttttgc
      121 ggaggcaggt cggtacaagt acccaatgcc acttccgccg ccataccgat ggattcgcca
      181 agggttgggt gcgggtggat ggttttaccg atatcggctg catcacagcc catttcgatg
      241 gccaagcaga cttcgccgat catgtcgcca ccgttcggac cgacaatgcc gccgccgatg
      301 atgcggccgg tttcggcatc gaaaatcagc ttggtaaagc cgttgtcgca accgttggca
      361 atcgcacgac cggaagccgc ccacgggaag ttggctttgg tgattttgcg gccggaggct
      421 ttggcggaca actcggtttc gcccacccac gccacttcgg gggaagtgta ggcaacgccc
      481 ggaatcacgc gcgcgtcgaa gtaggctttg tggccggcgc agttttcggc ggcaacgtgg
      541 ccttcgtgaa cggctttgtg cgccaacatc ggctgaccga cgatgtcgcc gatggcgtag
      601 atgtgcggca cattggtacg catttgtttg tccacttcga tgaagccgcg atcggtaacg
      661 gcaacgcctg ctttttctgc gctgatgagt ttgccgttag gcacacgacc ggcggcaacc
      721 agtacggcat cgtaacgttg tggctcttta ggcgcatttg caccttcaaa ggtaacgtaa
      781 acaccatctt ctttaggctc gactgcaacg gttttggtgt tgatcatgat gttatcaaaa
      841 cgatattcgt tttgtttttg ccatacttta accaagtcgc ggtctgcgcc ttgcatcagg
      901 ccgtccatca tttcaaccac atccaaacgc gaacccagcg tgctgtaaac cgtacccatc
      961 tcgaggccga taatgccgcc gccgataatc agcagtttgc ccggtacttc tttcagagcc
     1021 aatgcgccgc tggaatcgat gatgcgcgga tcttcaggaa tgaaaggcag tttggttacg
     1081 cggctgcctg ctgcaatgat acagtttttg aaggcaacga tttttttctc gccggtaggg
     1141 gctgcctgtt cgtacgcgtc gccggcagtc agcgacactt ccaagtggtg cggatctaag
     1201 aattgcccgt cgccttggat aacgtccact ttacggcttt tcgccatacc tgccaaaccg
     1261 cccgtgaggc gggaaaccac gccgtctttg taggcgcgaa gcatatcgat gtcgagttcc
     1321 ggctcggggt atttgatacc gttggcagcc aagtggcgca cttcgtcgat aacggcggca
     1381 ttgtgcaaca aggctttgga agggatacag ccgacgttca ggcaaacgcc gcccaaagtt
     1441 ttgtaacgtt cgacgatggc gactttcaag ccttcatcgg cagcggcaaa tgcagcggag
     1501 taaccgccgg gaccgccacc caatacgacc acgtcgtact cggcatcggc agaaccgccg
     1561 aattgcgcgg cttgcggagc aggagcggca gctttagggg cttcttgcgc cggggcggca
     1621 gccgcttcgg ctttaggggc ggcagccgca ccttcagctt cgacgacgac aatcaaaccg
     1681 ccttcggaga ttttgtcgcc gactttgact ttgacttctt tgaccacgcc cgcgacttcg
     1741 gcaggcacgt ccatcgtcgc tttgtcggtt tccaaggtaa tcagggtatc gtccacagca
     1801 atggtgtcgc ctacatttac ttcaaccgca ataatatcta cattttcgtg tccgccaatg
     1861 tcgggcactt tcaattcaac taaagccatt atattgacct ttcaacttag ttaatttcta
     1921 tcaactcttt agcaataatc acttcttcag gaatacagcc acaatcttca gctctgattg
     1981 ttacttcctt ctcatttggg ttttctggat gttcaacctc atattcaaaa atagaatatt
     2041 gaccaaacaa atccctattt aaaacatata tatagccatt ttcgatgccg gaacttgttg
     2101 caaatttctt ggcaatttcc ttgtctgttg tcgtagatat ataacatccg tcatatagac
     2161 ctgtttcaat ttgatgggcg taaactgcat tcttcacact tggaccatgt gtagctttac
     2221 catcatattt aaacttacca tcataacgaa ttgcaacttc agctttatta cctttaggtt
     2281 ttaactgtcc attattttgc tcatcttgtt ggcaactaat gcctctatat aagaaatttc
     2341 ccataagtta ttaatttaaa ggttgaattt aacaccatta ctcatcaatc tcaagcaatc
     2401 caaacattat cagactgctt gagagggatg ttttatttta taaggtaatg cggcggaagt
     2461 ctttcaacag cttcgccaag aatacggtga agcgcatacc ggccgcaccg tcgatgacac
     2521 ggtggtcgaa ggacaggctc aacgggcaca tcaggcgcgg ggcaaactct ttgccgttcc
     2581 aaacaggttt gatttgggat ttgcacacgc ccaagatggc gacttcggga gcgttcacaa
     2641 ttggcgtgaa gcctgtgccg ccgatgccgc ctaagctgga aatggtaaag cacgcgcctt
     2701 gcatttcttg cggtttgagc ttgccttcac gggctttttt ggacaattcg gtcaattctt
     2761 ggctgatttg tttcaagcct ttttgatcca cgtctttgat gacgggaaca accaagccgt
     2821 tcggcgtatc ggctgcgaaa ccgatgttga agtagttttt cagcaccagg ttgtcgccgt
     2881 ccagtgaggc gttgaattcg gggaatgctt tcaacgcgga aacagaggct ttgatgatga
     2941 acgccaacgg ggacagtttc acgccttcgc gttcccattc tttgttcagc tgtttgcgga
     3001 attcttccag ctcggtcatg tccgcttctt cgtgtacggt aacgtgggga atcacaaccc
     3061 agttgcggga caggttttga ccggaaattt tcttaatgcg ggacaattct ttaacttcga
     3121 cattgccgaa tttggagaag tccactttag gccacggcag taagtccaga ccgccgccca
     3181 aagatgcgct ggctgcggca ggttttgccg cgccgccctg catcacggat ttcacaaagg
     3241 ctttgatgtc gtcgcccatg atacggcctt tcaagccggt gcctttgact tggcccaaat
     3301 ccacgcccaa ttcgcgcgcc agtttgcgtg cggaaggacc ggcgtgtgct ttggcgaaag
     3361 cggcctcgtc gattttggca gcggcaggtg cagaaggtgc aggcgcggcg gcgggtgcgg
     3421 cggcagcagt cggaggcgga gcggcagccg gtgcggcagc ttgagcggct tgagcagggg
     3481 ctgctgcggc agagccgacg gtttctactt cgataatggc agagccttcg gatactttgt
     3541 cgccgacttt taagaatacg gctttaacga caccggcagc ggtacaaggt acgtccattg
     3601 tcgctttatc ggtttccaaa gtaatcagcg tgtcgtcttc ggcaacggtg tcgcccactt
     3661 tgatttcaac ggcgattaca tccacatcgg tatggccgcc gatatcggga acggctactt
     3721 gaacggttgc accgcctgcg gcagcgggtg cggcagcagg tgcaggttgt gcttcggcag
     3781 cagccggggc ggcttcggcg gcggcggcac cggtttcaac ggtcagaatt acgccgcctt
     3841 cggagatttt gtcgccgact ttgactttca cttctttcac gacaccggcc gcatcggcag
     3901 gcacatccat cgtggctttg tcggtttcca gtgtaatcag ggtgtcgtca acggcgatgg
     3961 tgtcgcccgc tttaacttct acggcgatga tgtcgacgtt ttcgtgaccg ccgatatcgg
     4021 ggactttgat ttctacgata ctcatttgag ttcctttaat cttgttcaat tggatgccgt
     4081 ctgaaagctg cttcaggcgg catcggtacg gtttgtctgc tcaaaccatt cgggcattcg
     4141 gttttcagac ggcctgatgt cggaatgggg caaacagacc gtctgaaaca tcaatcagcg
     4201 tttccagcta ggagctgaat cggctttgat gccgtatttc tcaatggctt gttgaacggt
     4261 ttctttgctg actttgcctt gttccgccaa tgcgctcaat gctgccacgg caacgttgta
     4321 gcgatccact tcaaagaagc ggcgcaggtt ggcgcggctg tcggagcggc cgaagccgtc
     4381 agtacccaag acgtggtagt cgttcgggat gtaggcgcgg atacggtcag catagctgcg
     4441 gatatagtcg gtagcggcaa taaccggacc gtcatgacct tgcaatttgg aagtaacgaa
     4501 aggtactttt tcggcttcca gcggatgcag gcggttgaag cgttctacct cgacagcgtc
     4561 gcggtgcagc aggttgaagg atgggcaaga ccaaatgtct gcttccacgc cgaagtcggc
     4621 tttcagcagc tcggcaccgg taaccacttc ttgcaaaatc gtaccggaac ccatcagttg
     4681 gactttctta tcgcctttgc cgccggcttt cagcaggtac atacctttca agatgtcttg
     4741 ttccgcacct tcgggcatat ccggatgggt gtagttctcg ttcatcaggg tgatgtagta
     4801 gaacacgtct tcattattgg catacatacg gcgcagaccg tcttgtacga tgacggcgac
     4861 ttcgtattgg aaagtcgggt cataagatac gcagttcgga atcaggtcgg cctggatgtg
     4921 gctgtggccg tcttcgtgtt gcagaccttc gccgttcagc gtcgtacggc cggcagtacc
     4981 acccaacagg aagccgcgtg cgtgcatatc acctgccgcc caagccaagt cgccgatacg
     5041 ttggaaaccg aacatagagt agtagatgta gaaaggaatc atcgcaaagt tgctgttggc
     5101 gtagctggtt gcagcagcga tccagtcagc cattgcgccc ggttcgttaa taccttcttg
     5161 caagatttga ccgtcaacgg attctttata gaacatcagt tggtctttgt cttgaggggt
     5221 atattgctga cctttcggat tccaaatacc gtattggcgg aacatgcctt ccataccgaa
     5281 agtacggctt tcgtcgggaa cgataggtac gacgcgtttg ccgatttttt tgtctttcaa
     5341 caaggttgac aaaatgcgga caaatgccat agtggttgag aattcgcggt cgccgctgga
     5401 tttcagttgg gtatcaaatg cggacagctc cggcacttcc aaaacttctt gggtaggttt
     5461 gcgttgcggc aggtagccgc ccaaagcatc gcggcgtgcg tgcaggtatt tgtattcttc
     5521 cgtatcgggg gcaaaagtca ggtaaggcag atcgccgctt tcgatttgtt cgtcggtaac
     5581 cggaatgtca aagcggtcgc ggaattgttt cagggacgct ttgtccattt ttttggcttg
     5641 gtgggcaacg ttctgacctt cgccggatgc gcccataccg taacctttaa tggttttcgc
     5701 caagatgacg gtaggtttgc cgtcggcatg gttcgctgcg cggtcgtagg cgttgtacac
     5761 tttttgcggg tcgtggccgc cgcggttcaa tgcccagagt tgctcatcgg tcatatcggc
     5821 aaccaatgct ttcagttcgg gcgtattgaa gaagtgttcg cgcacatacg cgccgtcttt
     5881 ggatttgtaa gtttggtagt cgccgtccaa acattcttcc atacgttggc gcaggatacc
     5941 gtctttgtct ttcgccaaga ggcggtccca gcggcggccc caaatgactt tgacgacatt
     6001 ccagccggcg ccggcaaagt tgccttccaa ttcttggatg attttgccgt tgccgcgtac
     6061 cggaccgtcc aagcgttgca gattgcagtt gatgacgaaa atcaggttgt ccaagccttc
     6121 gcgtgcagcc agtgcgattg caccttgaga ttcgggttcg tccatttcgc cgtcgccgca
     6181 gaaacaccat actttacggc ctttggtttt tgccaaacca cgcgattcca agtatttcag
     6241 gaaacgcgcc tgataaatcg ccatgatggg ccccaagccc atggatacgg tcgggaactg
     6301 ccaaaagtcg ggcaagaggt gggggtgcgg ataggaaggc agaccgtgtc cgtccacttc
     6361 ttggcggaag ttgttcagct ggtcttcggt cagacggccc tcgacgaatg cgcgtgcata
     6421 gatgcccgga gcgacgtgac cttggaagaa gaccaaatcg ccttcttcgc cttcgccttt
     6481 ggctttccaa aagtggttga aaccgacttc gtacatggtg gcggcagatt ggaaagatgc
     6541 gatgtgccca cccagttcca aatctttctt gccggcgcgc aatacgatgg cggcggcgtt
     6601 ccagcgcacg aatgcgcgga tgcggtgttc gatgttttgg tcgcccggaa tgcctttttc
     6661 gttttcaacc gaaacggtat tcaaatacgg ggtggtcgtg ccgtgtggca tacgtacgcc
     6721 cttgtcgcgg cagtatttga ccaggttttc caagaggtat tgtgcgcgtt cgccgccttc
     6781 atattcgagg acggagctta acgcgtccag ccactcttgg gtttcgatag ggtcaacatc
     6841 gtgtaattgg gtggacatag tatctatcct ttatgttgag tgtttaatcg gcagggaggg
     6901 tgtagccaac ccctttattt tcgtttgtga aaaataataa gcaaaaacga aaatttctaa
     6961 atcaaaccga aaaaaataat ttaagtttaa tgttttcaaa aaattataaa agttttggaa
     7021 acaagggaaa acttgaaaaa aatgttcgat ctgctccaca cgaaatccta tcagcaatcg
     7081 ggtctgctgg caaataacca tgcctgtaaa agcaggtttt atactgaagt tggcgtttgg
     7141 taaggtcaag tctttttgat ttcgaaatat tctgtttgat tttggcgata ttttttcgga
     7201 attgtggtat atcgaatcat tatgtctctg tcggcaattt tcctattgta gaaaatctat
     7261 acacaagctt tgtggataac cgtgttgata agccacggct tgtgttaaaa aagtcttacg
     7321 caacaaatgt acgcttattt tgattaaaaa ataggcacat ttaaatctat aaaaatcatg
     7381 tgcttatgtt caatatgatg tttctggtca tgtttttgcc gtctgttatg ggatttgtcc
     7441 cgcgcttggg gcagggggta aattttggag gttggtgcaa aacctgcttt tttcgcgtta
     7501 caatctgcac ccgatttact tttgaatttg cctgtttgga aaaatacttt tatgaaagcc
     7561 agccaattct ttatctctac tttaaaagaa gcccctgccg aagccgcgct tgccagccac
     7621 aagctgatga ttcgcgccgg tctgattaaa gccaacgcgt ccggccttta tacttggatg
     7681 ccgatggggc tgcgcgtgtt acgcaaagtc gaaaacgtcg tgcgcgagga aatggcgcgt
     7741 gcgggcagcg tggagttgct gatgccggtg gtgcagcctg ccgagctgtg gcaggaatcc
     7801 ggccgctggg agttttacgg taaagagttg ctgcgcctga aagaccgcca cgaccgcgat
     7861 ttctgcatgg gtccgacctg tgaagaagtc atcgccgaca tcgtgcgcaa agaaatcaac
     7921 agctacaaac agctgccgaa aaacttttac cacatccaaa ccaaattccg cgacgaagtg
     7981 cgcccgcgtt tcggcgtgat gcgcgcgcgc gagttcgtca tgaaagacgc ttattccttc
     8041 catgccgact acgcctcgct tcagacgacc tatgatgcca tgtatgacgc ttactgccgc
     8101 atcttcaccc gtctgggctt ggcattccgc cccgtcgccg cagacaccgg cagcatcggc
     8161 ggtacgggtt cgcacgagtt tcaagtgttg gcggaaagcg gcgaagatgt gattgcgtac
     8221 agcgacactt ccgattacgc cgccaatatc gagttggcac cgaccttgcc gcttaaaggc
     8281 gaacgcaccg ctgctcaggc cgagttgacc aaagtacata caccaaacgt caaaaccatt
     8341 gagtctttgg ttgaattcct gaatattccg gttgaacaaa ccctcaaatc catcgtggtt
     8401 gaaggcgaaa acgaaggcga aatcgtttta ttgctgttgc gcggcgacca tgagttcaac
     8461 gacatcaaag ccgaaaaact cgcaggtgtg aaatcgccat tgacgatggc aagccctgcc
     8521 gcgattgtcg aacagttcgg cgcaaacggc ggctcgctgg gtccggtcgg ctttgcaggt
     8581 aaagtttatg ccgatttcgc cactgaaaaa ggcgcggact gggtcatcgg tgcgaacgaa
     8641 gacggctacc actacaccgg cttcaacttc gggcgcgatg ccgccgagcc tgaattcgtc
     8701 gatttgcgta atgtggtcga aggcgacgaa agccccgacg gacaaggccg tctgaaactg
     8761 gcgcgcggca ttgaagtcgg acacgtcttc cagttgcgcg acaaatatac taaagccatg
     8821 aacgtcagct tcctcgacaa caacggcaaa tcgcaaatta tggaaatggg ctgctacggc
     8881 atcggcatca cccgcgtcgt tgccgccgcc atcgagcaga ataacgacga aaaaggcatc
     8941 atctggacca aagcaatggc gccgtttgaa gtcgttatcg tgccgatgaa ctacaaaaaa
     9001 tcagacaccg tacgcgaagc cgccgacaaa atctatgccg aattgctggc ggcaggcgcg
     9061 gatgtgctgc tggatgaccg cgacgaacgc gcaggcgtat tgctgaatga ctccgagctg
     9121 ttgggtattc cgcaccgcat cgtcatcggc gaccgcgctt tgaaagaagg caatgtcgaa
     9181 tacgccgaac gccgcaacaa cgaagcgcag gcagttgcaa tcggagaaat tgttgcgcgt
     9241 gtaacagctt cattaaatgg gtaagcttgg tcaaatgccg tctgaaaata atttcagacg
     9301 gcatttttat ttggggagaa agtgaagtgg cctcagtatt tttagaagga gaagcagtcg
     9361 aggtcggcaa tattttctgt atcggcagga actatgccgc acatattgaa gagttaaaaa
     9421 acgaaatacc ttccgaacct gtggtgttta tgaagccgtc aggcagcatt ttgaacagcg
     9481 gcggtacaat cttactgccc gagttcagta gggatgtgca gtttgagtgc gaactcgtgc
     9541 tgctggtcgg taaggattca gacggcacgg gcgagggtaa agatattttg ggatgtgtcg
     9601 caggatatgg tgtagggctg gaccttacgg cacgggatat ccaatgccgt ctgaaggaaa
     9661 aagggctgcc ctggctgaag gcaaaagggt tcaggcattc cgcgtgcgtg tcggattttg
     9721 cggcggcagg cagaatcggc aatcccgaga atgtgttgtt ttcattaaaa caaaacggcg
     9781 tattgaaaca gcgcggggat accggtttga tgatttaccc gattcgggaa attttacaca
     9841 aactggcggc agattacggt ttggggaaag gggatttggt gtttaccgga acgccctccg
     9901 gcgtgggcgc aatcggtgcg ggggacaatt tggcattgga gctggacggc ttggtgcgcg
     9961 cctctttcac ggtagggtgt taaccgcgcc gcttaacctt cacgtccgtt gaaacattcc
    10021 gccggaccgc cctcgaaaaa accgtgcaag atggcttgcg ccgccacttg gtcgagcacc
    10081 gatttgcgtt ttttgccgaa gacctgtgct tccgaaagca ggctttcggc atagacggac
    10141 gacagccgtt cgtcaaccca atagacgggg agattgaacc tgccgttcag cctgcgtccg
    10201 aacttgcgcg acaggtgcgt catttcatgt ttcgtgccgt cggtatgcac gggcagtccg
    10261 acgacaaaat aacgcggctg ccattcttga accagcttgg cgattgccgc gaacttttca
    10321 tcgttgctgc cgccggtaac ggtgctcaaa ggatgggata gccctaattc cgcgtctcct
    10381 tgtgccacgc cgatacgcgc ttcgccgaag tcgaatgcca gtgccgttcc ttttggaatt
    10441 ttatgcatgg ccggctcccg aaaacagggc gagcgggtcg atgccgagtt tggcgaatgc
    10501 ggcggcgtaa cggtgttcgt aggggatgtc gaacaggatg tgttcgtcgg cgggaacagt
    10561 cagccacgca ttgtcggcaa gttcgcgttc gagctgccct ttgctccagc ttgaatagcc
    10621 tatgctgatc aaggctttgt caaccgcacc ttcgcgtgaa atattttcaa tcacgtctcg
    10681 ggaagaagtt agcgcgatat tgtctgaaac gccgatactg ctttgccagt tgccgatcgg
    10741 ggtatgcacg acataaccgc gctcgacctg caccggaccg cccatcatca cgctgtcgtg
    10801 ctgcatccgc atggggatgt ttttgccggt ggcggaaaaa atcatgtcca tcgtaatcgg
    10861 agagggtttg ttgatggcga tgccgagtgc gccgtcttca tcgtgtttgc agatatagac
    10921 gaccgattgt gaaaaaaacg cgtcttccat atcgggcatg gcgatgagaa agtggttgga
    10981 gaggttcata tcagcgtttg tcgagatttt ctatcgggtt ggaaatcatg caggcgccgg
    11041 caagcaggtt gccggaagta tcggcttgca ccccatcggt ttgcggtatg ccgccgccga
    11101 aacaggaaac cgcgcttaag gaatttttcg gcgagccttg gatgatttta tcgctgtaca
    11161 ccaaataggc gaaggttttg cgtttggggt cgtaataacg gacaatctgc cggctcttga
    11221 acgcgaagct cgcaccgtgt ttgaaaactt ctttcggttt gcgcacggcg gtttcgtcaa
    11281 aagaaatcga agatgccgtc tgaacgcacg aaaccgatgc gtcggacgcg tcctcttcca
    11341 aattgaccat ttctttcaag ccgccttttt ttgcatacga aatataacag gcaacccctt
    11401 gaacgtcggg atcgtcgaat ccttccactt cgatacggtc gtttttgccc agcatgttga
    11461 aaacggtact tgcccgtccg attttatcgg tttcgccgct gccgcaggca gtcagcagga
    11521 cggcggcaga cagcagtagc agtctgttca tcaaaatttc cttatcggtt gaatccccgt
    11581 tttccgtgaa ggcggcaggg cttgctttgt ttggaggggc gcacgttccc aaaatcgggc
    11641 aacatacggt atgcggctta atgtgctacc ctgtgtgtcg gaacacaata tccgttgcgg
    11701 cattgaaaat atgacgcaca atataaggct gtttgagccg attgcaagta tccgcgcaaa
    11761 gaaggcgcaa gattgggcaa acggcatttt cagacggcat aactgacagt ataatccaaa
    11821 cgtccggctg ctttcgtgca gccttcagta ttatccaact ttttccgaag gtcgaaccat
    11881 gcgctacaaa ccccttctgc ttgccctgat gctcgttttt tccacgcccg ccgttgccgc
    11941 ccacgacgcg gcacacaacc gttccgccga agtgaaaaaa cagacgaaga acaaaaaaga
    12001 acagcccgaa gcggcggaag gcaaaaaaga aaaaggcaaa aatggcgcag tgaaagataa
    12061 aaaaacaggc ggcaaagagg cggcaaaaga gggcaaagag tccaaaaaaa ccgccaaaaa
    12121 ccgcaaagaa gcagagaagg aggcgacatc caggcagtct gcgcgcaaag gacgcgaagg
    12181 ggataagaaa tcgaaggcgg aacacaaaaa ggcacatggc aagcccgtgt ccggatccaa
    12241 agaaaaaaac gcaaaaacac agcctgaaaa caaacaaggc aaaaaagagg caaaaggaca
    12301 gggcaatccg cgcaagggcg gcaaggcgga aaaagacact gtttctgcaa ataaaaaagt
    12361 ccgttccgac aagaacggca aagcagtgaa acaggacaaa aaatacaggg aagagaaaaa
    12421 tgccaaaacc gattccgacg aattgaaagc cgccgttgcc gctgccacca atgatgtcga
    12481 aaacaaaaaa gccctgctca aacaaagcga aggaatgctg cttcatgtca gcaattccct
    12541 caaacagctt caggaagagc gtatccgcca agaacgtatc cgccaagagc gtatccgtca
    12601 ggcgcgcggc aaccttgctt ccgtcaaccg caaacagcgc gaggcttggg acaagttcca
    12661 aaaactcaat accgagctga accgtttgaa aacggaagtc gccgctacga aagcgcagat
    12721 ttcccgtttc gtatcgggga actataaaaa cagccagccg aatgcggttg ccctgttcct
    12781 gaaaaacgcc gaaccgggtc agaaaaaccg ctttttgcgt tatacgcgtt atgtaaacgc
    12841 ctccaatcgg gaagttgtca aggatttgga aaaacagcag aaggctttgg cggtacaaga
    12901 gcagaaaatc aacaatgagc ttgcccgttt gaagaaaatt caggcaaacg tgcaatccct
    12961 gctgaaaaaa cagggtgtaa ccgatgcggc ggaacagacg gaaagccgca gacagaatgc
    13021 caaaatcgcc aaagatgccc gaaaactgct ggaacagaaa gggaacgagc agcagctgaa
    13081 caagctcttg agcaatttgg agaagaaaaa ggccgaacac cgcattcagg atgcggaagc
    13141 aaaaagaaaa ttggctgaag ccagactggc ggcagccgaa aaagccagaa aagaagcggc
    13201 gcagcagaag gctgaagcac gacgtgcgga aatgtccaac ctgaccgccg aagacaggaa
    13261 catccaagcg ccttcggtta tgggtatcgg cagtgccgac ggtttcagcc gcatgcaggg
    13321 acgtttgaaa aaaccggttg acggtgtgcc gaccggactt ttcgggcaga accggagcgg
    13381 cggcgatgtt tggaaaggcg tgttctattc cactgcaccg gcaacggttg aaagcattgc
    13441 gccgggaacg gtaagctatg cggacgagtt ggacggctac ggcaaagtgg tcgtggtcga
    13501 tcacggcgag aactacatca gcatctatgc cggtttgagc gaaatttccg tcggcaaggg
    13561 ttatatggtc gcggcaggaa gcaaaatcgg ctcgagcggg tcgctgccag acggggaaga
    13621 ggggctttac ctgcaaatac gttatcaagg tcaggtattg aacccttcga gctggatacg
    13681 ttgatcggat tgccgaaacg gtaggcttta tgcaaatcgt gccgatatgg tgcgctgttc
    13741 cgtgaagttt gccgtacctg tattttccgg tttttggaaa tcaagagaga aagttaaaga
    13801 agaatgtcga aacctgtttt taagaaaatc gcactttata ctttgggtgc aatcagcggc
    13861 gtggccgtca gtctggcggt gcagggtttt gccgccgaga aggacaggcg ggataacgaa
    13921 gtcctgccgg tgcaatccat ccgcacaatg gcggaggttt acggtcaaat caaggcaaac
    13981 tactatcagg acaaacccga tgccgatttg tttgaaggtg cgatgaaggg tatggtggcc
    14041 ggtttggatc cgcattccga atatatggat aaaaaaggtt atgccgagat aaaggagtcc
    14101 accagcggcg aatttggcgg cttggggatg gaaatcgggc aggaagacgg atttgtcaaa
    14161 gtggtttcgc cgattgagga cacgcctgcg gaacgggcgg gggtgaaaag cggcgatttc
    14221 attgtgaaaa tcgataatgt ttcgacgcgg gggatgacag tcagcgaagc ggtgaagaaa
    14281 atgcggggca agccgggtac gaagattact ttgacgctgt cgcgcaaaaa tgccgacaag
    14341 ccgatagtcg tcaacctgac ccgtgccatt attaaagtga aaagcgtccg ccatcacctg
    14401 atcgaacccg attacggcta tatccgcgtg tcgcagttcc aagagcggac ggtcgaaagc
    14461 gtcaataccg ccgcaaaaga gctggtaaag gaaaataaag gaaaaccgct caaggggctg
    14521 gtgttggatt tgcgcgacga ccccggcggg cttttgaacg gcgcggtcgg cgtgtcggcg
    14581 gcgtttttgc cgcctgatgc gctcgttgtc agtacgaaag ggcgggaagg tacggaaggc
    14641 tttgcgttaa aggcgcgtcc cgaagattat atttttaaag ccggcacgga tcctttggcg
    14701 ggtattcctg ccgagttgaa aaccataccg atgacggttc tgattaattc gggttcggct
    14761 tccgcgtcgg agattgtcgc aggtgcattg caggatcata aacgcgcggt tatcgtcggt
    14821 acgcagagct tcggcaaagg ttcggttcag actttgattc ctttgtccaa cggcagcgcg
    14881 gtcaagctga caacggcgct gtattatacg ccgaacgacc gttctattca ggcgcagggg
    14941 attgttcccg atgtcgaagt aaaagataag gaacgcattt ttgaaagccg cgaggcggat
    15001 ttggtcggac acatcggcaa tcctttgggc ggcgaggatg tgaacagtga aacccttgcc
    15061 gtgccgcttg aaaaagatgc ggataagccc gctgtaaaag aaaaaggtaa aaagaaaaag
    15121 gacgaggatt tgtcttcaag gcggattccc aaccctgcca aagacgacca gttgcggaaa
    15181 gctttggatt tagtcaagtc gcccgagcag tggcagaagt ctttggggct ggcggcgaaa
    15241 aagccggttt caaataaaga taagaaagat aaaaaagata agaagtaggt ttttacgatg
    15301 ccgtctgaaa ggatttcaga cggcatatta tgtttgaaag gacaggttat gagcgatttt
    15361 ttaccggaat ttgaaccgtg cgg
//
LOCUS       DAXHNE010000034        14908 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.034, whole genome shotgun sequence.
ACCESSION   DAXHNE010000034 DAXHNE010000000
VERSION     DAXHNE010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14908
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.034"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(94..3579)
                     /gene="smc"
                     /locus_tag="ACFM4Z_000819"
     CDS             complement(94..3579)
                     /gene="smc"
                     /locus_tag="ACFM4Z_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFC6020884.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELGNKRGVLREQIARLEEQIRHRQNLHQRIERDKQAAQAQLQRIHQE
                     QQQIRVQLEENELYVEEKQTELAEWAMQVAEHEERLPELEEAQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHTGQTIAKHEECKGRLKQENQALNLPDEAETAAAQEAAALLQSQ
                     QEHYEEQIIAAEETLHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARSVPQGFVPPAPLPQGQAAWL
                     SDDLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQNNLSAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELESLAPELSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLLDDISTLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYQQQIARLEQQTLDWQERQQELVLAYETEFQNDEQHIKLDELT
                     ESVHTLDEEYIAVQEKLTQIQEQGREQYARVQTLQTQLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITLLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            3563..3832
                     /locus_tag="ACFM4Z_000820"
     CDS             3563..3832
                     /locus_tag="ACFM4Z_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020885.1"
                     /translation="MWVRRIMVGKNKKEAYFNGNPAAPPHILPAKPYRIPHPRCRLKP
                     SDGIFNALEPRFRQCPMLGAAFAVLPRRRARYNPPRHPPDLETCL"
     gene            3829..4551
                     /gene="lpxH"
                     /locus_tag="ACFM4Z_000821"
     CDS             3829..4551
                     /gene="lpxH"
                     /locus_tag="ACFM4Z_000821"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFC6020886.1"
                     /translation="MKPAYFISDLHLSEKHPELTALLLRFLRSSAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYLRFRRIVHCRRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKC"
     gene            4820..6406
                     /locus_tag="ACFM4Z_000822"
     CDS             4820..6406
                     /locus_tag="ACFM4Z_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFC6020887.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     DDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(6574..6777)
                     /locus_tag="ACFM4Z_000823"
     CDS             complement(6574..6777)
                     /locus_tag="ACFM4Z_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HFC6020888.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            6893..7390
                     /locus_tag="ACFM4Z_000824"
     CDS             6893..7390
                     /locus_tag="ACFM4Z_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HFC6020889.1"
                     /translation="MTALTLPEDIRQQEPSALLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            7472..8665
                     /locus_tag="ACFM4Z_000825"
     CDS             7472..8665
                     /locus_tag="ACFM4Z_000825"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HFC6020890.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVGLLKAKG
                     ASQNFDFIIKQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(9068..10003)
                     /gene="hemC"
                     /locus_tag="ACFM4Z_000826"
     CDS             complement(9068..10003)
                     /gene="hemC"
                     /locus_tag="ACFM4Z_000826"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFC6020891.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYTRLEEMPEGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDGRIRMILSESESLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHGVTNACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLRADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            10122..10637
                     /gene="ybeY"
                     /locus_tag="ACFM4Z_000827"
     CDS             10122..10637
                     /gene="ybeY"
                     /locus_tag="ACFM4Z_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFC6020892.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGTLHLMGYDHIEDDEAEIMEAEEIRLMLAA
                     GFPNPYQEDEY"
     gene            10639..11463
                     /locus_tag="ACFM4Z_000828"
     CDS             10639..11463
                     /locus_tag="ACFM4Z_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFC6020893.1"
                     /translation="MDGTQSKPKFFERLISRLAGEPDSAEDVLTLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(11592..12971)
                     /gene="nhaC"
                     /locus_tag="ACFM4Z_000829"
     CDS             complement(11592..12971)
                     /gene="nhaC"
                     /locus_tag="ACFM4Z_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFC6020894.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPNVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVILALMRINAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVVKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAIRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDRLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(13262..14485)
                     /locus_tag="ACFM4Z_000830"
     CDS             complement(13262..14485)
                     /locus_tag="ACFM4Z_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HFC6020895.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         3515 a         4195 c         3837 g         3361 t
ORIGIN      
        1 gtaccgattt aaatttaatt cactataccg tctgaaaacc tgtatcgcaa cacgtcatta
       61 agaacggcaa gattcaaccg ttctgcaacc cgttcaaacg gcttccgcca tttccaacgc
      121 ttgtttgatg tccacggcga cgacgcgcga tacgcctttt tcctgcatgg ttacgccgac
      181 gagctgctcc gccatttcca tggtcaggcg gttgtgggag atgtagagga actgggtttg
      241 cgccgacatt tctttgacca ggttgcagaa acgcgaggtg ttggcatcgt ccagcggagc
      301 gtcgacttcg tccaaaaggc agaacggagc aggattgagg ctgaacaggg cgaacacgag
      361 gctcatggcg gtgagggctt tttcgccgcc ggagaggagg tggatggtgc tgtttttctt
      421 gccgggcgga cgcgccatga tggatacgcc tgcggtcagg aggtcgtcgc ctatcatttt
      481 gagggtggct tcgccgccgc cgaacagggt ggggaagaag gtttggactt tgccgttgac
      541 ggcgtcgaag gtttctttga aacgcgcttt ggttttgtcg tcgatttggg cgatggcttc
      601 ttccaaaagg gtgatggctg cctgcacgtc ttcgctctgg ctgcggtagt agccgtcgcg
      661 ttcgcgcgct tcttcgagtt cttgcagggc ggcgaggttg acggcgccga gggcttcgat
      721 ttgttgcgag aggctgccga tgctgctgtt caatactttc ggcgattctt tcgccaacgc
      781 ttcgagcgca tccaaatcgg cggcgcgttc ggtcaggttt tgatggtagc gtttggcgtt
      841 gatcagggct tcctgttgtt gcaacagggc ggtttgggtg gcggcctgaa gctgtggcaa
      901 ctgggtttgc agggtttgta cgcgggcgta ttgttcccta ccctgctcct gaatctgcgt
      961 gagtttttcc tgcacggcga tgtattcttc gtccagcgtg tgtacggatt cggtcagctc
     1021 gtcgagcttg atgtgctgct cgtcgttttg gaactcggtt tcataggcga ggacaagctc
     1081 ttgctggcgt tcctgccagt ctagggtttg ctgttcgagt cgggcgattt gctgctggta
     1141 gttttgtttt tgctggttga gcttgtggac ggcgacttcg gcaagcccgt attggcggtt
     1201 ggcttccaac agggcaagct gcgcctgttt cagacggcct tgctgctctt ggcggctgtg
     1261 tgcggtggtt tgctgttggt gttcaagttc ggcggcggct tcctgcaaag tagagatgtc
     1321 gtctaaaagg ccgtcggacg tgtgttgcaa cacggtctgt tcttccgcca actgcgccag
     1381 ttcgcgctcg atgtgttcgc ggcggatttg cccttggttg gtacgcgcca gaagttcggc
     1441 ggcacgttgc tgcgcttggc tgtattggcg cgtgtgctgc tgttgttgct gcatcaggtt
     1501 tttgtgctgc acttcggacg agcgcacggc ggcttccgcc tgtttgaacg cggcttcggc
     1561 ggcagaaagt tcgggggcga ggctttccag ttcggaagcg atgccgtcga ggcgcgcttt
     1621 ttgggcaatc aggctttcct gcgcgggttt ggcatagagc aggacgctga ctttgtcgac
     1681 ctgatggcct tcaggcgtga gccagatttg gtgtgcgctc aaattgtttt gatgcgcgag
     1741 ggcgtagctc aaatcgggtg cgcacaatac gccgtcgagc cagtggtgca atgccgtctg
     1801 aaacggcggc tgcgcttgga tttggttcag caatgcctgt acgggcaggg attttttgat
     1861 gccgccggag aggtcgtctg aaagccacgc cgcctgccct tgcggcaatg gcgcgggcgg
     1921 aacgaaacct tgcggcacgc tacgggcgtg caggcgttcg gcaaggatga cggacaaggc
     1981 gtgctgccac tcggcgggcg cggtgatgtg ttgccacagt tgcggcgcgg cggcgtggtc
     2041 ggttgcctgc cagaagtcgg cggcttcctg ctgttgcgac aggatttgcg acaacgcctg
     2101 ctgctgcgcc tgcaaggtga tgtgttgctg cttcaggctt tggaagcggt ttgaggccgt
     2161 ctgaaacgcc tcgcgggcgg cgtgtaaggt ttcttcggcg gcaatgattt gttcttcgta
     2221 atgctcttgc tggctttgca acaaggcggc ggcttcctgc gcggcggcgg tttccgcttc
     2281 gtcgggcagg ttcagggctt ggttttcctg tttcagacgg cctttgcact cttcgtgctt
     2341 ggcaatcgtt tgtccggtat gggcaagctg ctgctgcttc aacgccagtt cgcggcggat
     2401 gcggtttgcc tcgtcctgct gggtttggaa ggcggcgttg agcgtggctt gggcttcttc
     2461 caattcgggc agacgctctt cgtgttcggc aacctgcatc gcccactccg ccagctcggt
     2521 ttgcttttct tcgacgtata actcgttttc ttcaagctgc acgcggattt gctgctgctc
     2581 ttgatgaata cgctgcaact gcgcctgtgc cgcctgcttg tcgcgttcga tgcgttgatg
     2641 caggttttgc cggtggcgga tttgttcttc caaacgggca atctgctcgc gcaacacgcc
     2701 gcgtttgttg cccaattcgt gtaccgcctg ctgctgcgac tgttcggcgg tctgcaaggc
     2761 atgtacttcg tcgtttaacg cctgaacctg cgcggcggtt tcgtcctgct gcgcctgcaa
     2821 agactgatgc tgcgcggtcg ctttgtcggc ggcggcaagc gattgctgcc attgggcgta
     2881 atcgagcaaa tcctgttgtt ggttcaactg cgcggtcagg gatttgtagc gttcggcagt
     2941 ttcggcttgt ttttccagct tttccacctg acgcgccaac tcgttctgca aatcgcccaa
     3001 acgctgcaaa tgctcgcgcg tgtctttcag acgaccctcc gtctccttgc ggcgttcctt
     3061 atatttggac acgcccgccg cctcctcgat ataagcgcgc aactcctccg gccgcgcttc
     3121 gatgatgcgc gaaatcatcc cctgctcgat aacggcataa ccgcgcgcgc ccacgcccgt
     3181 acccagaaac aaatcagtaa tgtcgcggcg gcgcacggtc tgattgttga tgaaatatgt
     3241 cgattcgccc tgacgcgtca actgccgctt gatgctcacc tcggcatact gcccccacgc
     3301 cccctgcaaa ctgtggtcgc tgttgtcaaa caccagctcc accgaagccc tcggcgcagg
     3361 acggcgcgtc gccgcaccgt taaaaatcac gtcctgcata ctctcgccac gaagctgctt
     3421 cgccgaagcc tcgcccaaca cccagcgcac cgcgtcaatc acattcgact tgccgcagcc
     3481 gttcggcccg ataaccgcaa caagctgccc cggaacatga atcgtggtcg ggtcggtaaa
     3541 agatttgaag ccggagagtt tgatgtgggt caggcgcata atggtcggaa aaaataaaaa
     3601 agaagcgtat tttaacggaa atcccgccgc accaccccat atcttgccgg caaaacctta
     3661 ccgcatcccg cacccgcgat gccgtctgaa gccttcagac ggcattttta acgcgctcga
     3721 accccggttc cgccaatgcc cgatgcttgg cgcggcattt gccgtattgc cccgccgccg
     3781 ggcgcgttat aatccgccac gacatccgcc cgatttggaa acctgcctat gaaacccgcc
     3841 tatttcatct ccgacctgca tttgagcgaa aagcaccccg aactgaccgc gctgctgctg
     3901 cgttttttgc gttcttccgc cgccgggcag gcgcgggcgg tttatatttt gggcgatttg
     3961 tttgattttt gggtgggcga tgacgaagtt tccgagttga atacttcggt tgcgcgtgaa
     4021 atcaggaaat tgtccgacaa aggcgttgcc gtgttcttcg tcaggggcaa ccgcgacttc
     4081 ctgatcggtc aggatttttg ccggcaggcg ggtatgacgc tgctgccgga ttactcggtt
     4141 ttggacttgt tcggctgcaa aaccctcatc tgccacggcg acactctgtg tacggatgac
     4201 agggcatacc tgcgtttccg ccgcatcgtg cattgcaggc ggctgcaaaa actgttcctg
     4261 atgctgcccc tgaaatggcg cacgcgcctt gccgccaaaa tcaggcgtgt cagcaaaatg
     4321 gaaaaacagg tcaagcccgc cgatattatg gatgtcaatg ccgcctttac cgcgcggcag
     4381 gttcgcgcct ttaacgcgga aaggctgata cacgggcaca cccaccgcga gcatatccat
     4441 cacgaaaacg gctttacccg catcgttttg ggcgactggc ataacgacta tgcttcaatc
     4501 ctccgcgtag acggggacgg cgcggtattc gtgccgctgg aaaaatgctg aaaatgccgt
     4561 ctcaagcctt tcgggcggct ttttttacac gcccctttcc cacactccct ccgccgcatc
     4621 atcaccatcc ttaacccgca ttaaggggcg ttatcgataa aatgcttgac cgattacata
     4681 agattacgta aagtgtgcaa acgcataaca attggtctta ccaatttatc ttttaaccaa
     4741 aatatcgggt gtacgggatt tgaacgtctt tccaaacctc gacgcgcctt atgatgcaaa
     4801 tgcctaaaaa ggattcgtta tggcactttt cctcagcata ttccccatcg tcctgctgat
     4861 ttggctgatg gtgaaaaaaa acagtatgcc ctcctacgtc gcgctgccga ttaccgcagt
     4921 gctgatttac gccatcaaac ttttctactt cgacgatgcg ggcatgctgc tcaacgccac
     4981 cgccgcttcc ggcctcgtca aaacgctcac gccgattacc gtgattttcg gcgcgattat
     5041 gttcaaccgt atgatggaaa ccacgggctg catcgatgtc atccgcaaat ggctggcgac
     5101 catcagcccc aaccccgtag cgcaactgat gattatcggc tgggcttttg cctttatgat
     5161 tgaaggcgca tccggcttcg gcacgcctgc cgcgattgcc gcgccgattc tgatgagctt
     5221 gggcttcaac ccgttaaaag tggcgatttt cactttggtg atgaactccg tccccgtctc
     5281 tttcggcgcg gtcggtacgc cgacctggtt cggtttcgca ccgctgaacc tgagtgccga
     5341 agacatcctc gccatcggca ggcagaccgg cgtaatgcac ttcttcgcag gtttcgtcat
     5401 ccccgtcatc ggcttgggct tcatcgtacc ttggtctgaa atccgcaaaa acttgggctt
     5461 cgtcgccatt gccgtcttct cctgcaccat tccttatgtc gcattggcga tggtcaacga
     5521 agaattcccg tcgctcgtcg ccggcgcaat cggcctgatg gtgtccgtat tcgccgccaa
     5581 ccaaggctgg ggcttgagca aagaccacgc caaagacccg aatgccgaaa aagtgccgtt
     5641 cgcccaagtc gccaaagcac tcgccccttt gggtatgctg atcggcatgc tggtggttac
     5701 gcgcatcaaa cagctcggca tcaaaggcat tttgaccagc aaagaagaat ggttcagctt
     5761 ccaactgccg tttgatttgt ccaaaatcac cgtcagcgac tccctgacga ttaccttcgg
     5821 caatattttc ggacaagatg tcagcgcgtc ttaccaaacg ctgtacgtcc cggcttggat
     5881 tccgtttgtg ctgaccgttt ggatttgcat cctgctgtat aaaaccaaat tcaaagatgc
     5941 ctggacgatt tattccgtaa ctttcaatca aaccaaaaaa ccgctgcttg ccctgatggg
     6001 cgcgctgatt atggttcagc tgatgctggt cggcggcgac aattcgatgg tgaaaatcat
     6061 cggtaaggaa tttgccgcaa tggcgggcga acactgggtt tatttctcgc cgtatctggg
     6121 cgcgatcggt gcgttcttct ccggctccaa caccgtgtcc aacctgacct tcggaccgat
     6181 tcagcagcaa atcgccttgg ataccggcct gtccgttacc ctgattctgg cgttgcagtc
     6241 cgtcggcggc gcgatgggca atatggtgtg cctcaacaac atcatcgccg tatgtaccgt
     6301 attggatgtg aaaaattccg aaggtgcgat tatcaagaaa accgttatcc cgatggcgat
     6361 ttacggcgtg attgccgtcg tcgcggcaat gattttcttc ctctaggcga aacgccacca
     6421 tccatagcgg cagcacgcgg ggaacggaaa ccgccgttcc ccgtccgggc ggcgcgtcgg
     6481 gcagaatctc ggtgccggaa cagcccgccc cgccaaacaa atgccgtctg aaaccggaaa
     6541 aggcttcaga cggcattttt ccgccgcttg cgttcaggcg taatgatgat gcggcatctt
     6601 gccgtcaagg cggtagcgcg tgccgcaata cgggcaggca acgctgcccg attctccttc
     6661 gcacaaaggc aaaaacactc tcggatgccc gttccactgc tcgttgtcgg gtcccgagca
     6721 atacagcggc agattttccg gcaacacgga aatttcctgc agattcagat tgtccatttg
     6781 attttccttt gcgtggttgg cgtgcgccca ttttacgcca tcggcaggct aaaggatatt
     6841 ttcggcgcaa agccgcaatc cgctataatc cccacttttc agacggcata ccatgactgc
     6901 gcttaccctt ccggaagaca tccgccaaca agagccatcc gccctgctct ataccctcgt
     6961 ttccgcctac ctcgaacaca ccgcccaaac cggcgacgaa tccctctcct gcctgtccga
     7021 cgaccagcac acgctgaccg cattctgcta cctcgacagc caagtcgaag aaggcggctt
     7081 cgtgcaactc atcgcctccg gttacggcga atatattttc cgcaacccgc ttgccgacag
     7141 cctgcgccgc tggaaaatca aagccgtgcc gaaagtcttg gacaaagcca aagccctcta
     7201 cgaacaacac ggcaaaacca tcgaaacgct cgccgacgga ggcgcagaca tcgaatccct
     7261 gcgcaaacag ttccccgact ttgaagaatg ggacggcgca tactacgaag ccgccgaaca
     7321 agacctgccc ctgcttgcag aacacataca gtcaaactgg gaaaccttcg cccatatcgg
     7381 gcaggcgtga ttgcgtctgt ttccagcccg tgtaaaacag cgtaaaatcg gcaaacccga
     7441 atgaccttcc gtccccatca aggagcaagc tatgttcttc aagcacatcg aagccgcccc
     7501 cgccgatccg attctcggtt tgggcgaagc gttcaaagcc gaaacccgcc ccgaaaaagt
     7561 caacctcggc atcggcgttt ataaagacgc atccggcgcg acacccctcg tcaaagccgt
     7621 caaagaagcc gaaaaacgcc tgttggaaag cgaaaccacc aaaaactacc tcaccatcga
     7681 cggcgttgcc gactacaacg cgcaaaccca aatcctgctg ttcggcaaag accacgaaat
     7741 catcgccagc cgtcgcgcca aaacagcgca aagcctcggc ggtacgggcg cattgcgtat
     7801 tgcggccgag tttgccaaac gccagttgaa cgcgcaaacc atctggattt ccaatccgac
     7861 ttggcccaac cacaacgcca tcgccaaagc ggtcggtatc caagacaaac cttatcgtta
     7921 ctatgatgcc gccaaacacg gtttggattg ggacggcatg attgaggact tgagccaagc
     7981 gcaaaaaggc gacatcgtcc tgctgcacgg ctgctgccac aatcctaccg gcatcgaccc
     8041 tacgcccgaa caatgggaaa ctttggcaaa actttctgcc gaaaaaggct ggttgccgct
     8101 gtttgacttt gcctaccaag gcttcggcaa tggtttggaa gaagatgcct acggcttgcg
     8161 cgtgttcttg aaacacaata cagaattgct gattgccagc tcttattcca aaaacttcgg
     8221 tatgtacaac gagcgcgtcg gcgcgttcac tttggtggcc gaagatgaag aaacagcagc
     8281 ccgcgcccac agccaagtca aaaccatcat ccgtaccttg tattccaacc cggcttcaca
     8341 cggtgcgaac accattgcgc tggtgttgaa aaatgatgat ttgaaagcac aatggattgc
     8401 cgaactcgat gaaatgcgcg gccgcatcaa agccatgcgc caaaaatttg tcgggttgct
     8461 caaagccaaa ggtgcaagcc aaaactttga tttcattatc aaacaaaacg gtatgttctc
     8521 tttcagcggc ttgactcccg aacaagtcga ccgcctgaaa aacgagtttg ccatttatgc
     8581 cgtccgctcc ggccgcatca acgtcgccgg cattaccgac gacaacatcg attatctgtg
     8641 cgaaagcatc gtgaaagtct tgtaaaacaa aagcgcatac gaaatgccgt ctgaacctta
     8701 accagttcag acggcatctg ttttttgtta aaaaacaata aactataaca atttcaaaca
     8761 agctaaaaac atctataaaa tcaacccaat atgatgaact caaataaaaa atacgaatat
     8821 catcattctc aaattcaaaa tttgaacgcc acagtattat ccgaagccac aaaaaccttt
     8881 ccgccgtcat tcccgcgcag gcgggaatcc gggaacccaa tgctgcaaga attgattaga
     8941 aacaactgaa accgagcaaa ctggattccc gcctgcgcgg gaatgacgaa ataaggcgag
     9001 taaaactgat gaaacaaatt gattaaaaac aataacttat attattttat ttaaattttc
     9061 gataaaatca attttccgta ttcaatactg ctccaatcaa ttcccgcgca ccgtcgtccg
     9121 ccaatttctt agcgacagcg cgtccgagcg cgtcggcata ttcggcaggg gcttgcgcgt
     9181 ccgcccgcaa cacaaccgaa ccgtcgggat gtccgaccag tccgcgcaag gtcagcaagc
     9241 cgttttcttc tgtgcaatat gcggccaaag gcacttggca gcttccgccc aaagcgcgtg
     9301 cgagggcgcg ttcggcggta acgcaggcat tggtaacgcc gtggttcaag ggtttcaaaa
     9361 cttcatacaa atcttcgcgg tgcgcggcaa tttcgatacc caatgcgcct tgtccggcgg
     9421 caggcaggct ctcggattcc gacaaaatca tgcggatgcg tccgtccaat tctaaacgct
     9481 gcaaaccggc ggcggccaag ataattgcat cgtattcgcc gttatcgagt ttggacaaac
     9541 gggtttgcac attgccgcgc aaaggtttga taagcaaatg cggatagcgc gcacgcaact
     9601 gggcttcgcg gcgcaggctg gatgtgccaa caaccgcgcc ttcgggcatt tcttccaaac
     9661 gcgtgtattg gttggacaca aatgcgtcaa acggattggc gcgttcgccg atggccgcaa
     9721 gcgcgaaacc ttcaggcaaa tccatcggta cgtctttaat cgaatgcacc gccaaatcgg
     9781 cgcgcccgtc atataaagcc tgttccaact ctttgacaaa caagccttta ccgccgactt
     9841 ttgacaaagt tttgtccaaa atctgatcgc cgcgcgtggt catgcccaaa atctcgactt
     9901 cgcaatcggg atacagagcc ttcagacggc cttggatatg ctttgcctgc cacatagcaa
     9961 gcaggctttc gcggctggcg atgacgagtt ttttcgggtt catgttcaat gcgttcaaag
    10021 aatggaaacg gcgtgaagtc tatcatattt tgaacgtttc ttcagacgac accgtcaggt
    10081 ctatgttata tttacaccca caaaacacga aacccgccac aatgaaacgc accaaaaaat
    10141 accctttctt aacgttgcag cggcaacgtt tccatttgaa ctttgaaaac gcctcttccg
    10201 ccgccggcat cccagccgaa cgcgatttct accgctgggc gtggtctgcc ttgaaaaatg
    10261 aataccgccg cgccgacatc agcctgattc ttctggacga agaagaagcc cgagcctaca
    10321 accgcgacta ccgcggcaaa gattacgcca ccaatgtatt gagtttcgcg ctcaacgaag
    10381 gcgaaatcct gccctgccaa gtttcggaaa aactgtacgg cgatttgatt atctgcccgc
    10441 aagtcgtttt aaaagaagcc gccgaacagg gcaaaacgcc cgaacggcat tttgcccacc
    10501 tgaccataca cggtacttta cacctgatgg gctacgacca catcgaagac gacgaagccg
    10561 aaataatgga agccgaagaa atccgcctga tgctggcggc aggcttcccc aacccctacc
    10621 aagaggacga atattaaaat ggacggtacg cagtcgaaac ccaagttttt cgaacgcctg
    10681 atctcccgac ttgccggcga gcccgactcc gccgaagacg tattgaccct gctgcgccaa
    10741 gcgcacgagc aggaagtttt tgatgcggat acgcttttaa ggttggaaaa agtcctcgat
    10801 ttttccgatt tggaagtgcg cgatgccatg attacgcgca gccgtatgaa cgttttaaaa
    10861 gaaaacgaca gcatcgagcg catcaccgcc tacgttatcg ataccgccca ttcgcgcttc
    10921 cccgtcatcg gcgaagacaa agacgaagtt ttgggcattt tgcacgccaa agacctgctc
    10981 aaatatatgt ttaaccccga gcagttccac ctcaaatcca ttctccgccc cgccgtcttc
    11041 gtccccgaag gcaaatcgct gaccgccctt ttaaaagagt tccgcgaaca gcgcaaccat
    11101 atggcgattg tcatcgacga atacggcggc acatccggct tggtcacctt tgaagacatc
    11161 atcgagcaaa tcgtcggcga aatcgaagac gagtttgacg aagacgatag cgccgacaat
    11221 atccatgccg tttcttccga acgctggcgc atccatgcag ctaccgaaat cgaagacatc
    11281 aacaccttct tcggcacgga atacagcagc gaagaagccg acaccattgg cggcctggtc
    11341 attcaagagt tgggacatct gcccgtgcgc ggcgaaaaag tccttatcgg cggtttgcag
    11401 ttcaccgtcg cacgcgccga caaccgccgc ctgcatacgc tgatggcgac ccgcgtgaag
    11461 taagcaccgc cgtttctgca cagtttagga tgacggtacg ggcgttttct gtttcaatcc
    11521 gccccatccg ccaaacataa aaatgccgtc tgaaaccgtt gcaagtttca gacggcattt
    11581 cgcttatccg cttatttttt gctcaaagtc agccccgtcc aaccgaataa cagggtcaaa
    11641 gccaaactca aatagcagaa aaaggcatac ggcagatatt cccaaaccgg cacgcccagc
    11701 gcgtggctga tgaacacgcc gcatacgctc cacggtacga gcgggttaat caccgtcccc
    11761 gcatcttcca gcgtccgcga cagattgcgg gaatgtagtc cgagcctgtc gtaaacgggt
    11821 ttaaacgttt ctccagaaag caggatgctc aaatattgtt cgccaatcag gaaattaacc
    11881 ccgaccgaag tcatggcaac gctgaacgtc gcgcgtccgg cattcgtcaa gaaggtacgg
    11941 atggcctcca acagggaagg aatcacaccg agcgcaaaca acagtccgcc caaactcatc
    12001 ccgagaatca cgatggtttg cgtgaaaaac atactttcca aaccgccgcg cgaaatcagt
    12061 ttgacaacat ctttaaacgc ttcgccttcg agtttgtagc cgccgtaaaa ccacgcaccg
    12121 agctgacgca gatcgggcgt gctgtgcaga tacgttacag caacggcaac catgacggta
    12181 aagagcatgg cgacgacggc gttgatgcgc atcaatgcca aaatgaccaa cagcgcaaac
    12241 ggaatcagcg aatagccgtg caccaatccc gtggcttcaa gctggctgcg gaaggattcg
    12301 acgctgttca aatcctgcgc ggcgacattc ggcaaaagcc aaagcatcag tgccgcacta
    12361 atgagccacg cggggatggt ggtgtacatc atatttttga tgtgctcaaa caagtcgatg
    12421 ccgacgatgg acgcggaaat acccgtcgta tccgaaagcg gggacatttt gtcgccaaaa
    12481 aatgcgcccg aaacaatcgc gcccgccgtc atcgccatat cggcctgaaa cgccgccgcc
    12541 atccccataa aggcaacgcc gacagtggcg caggtggtca ggctgctgcc gatggacacg
    12601 ccgatgacgg aacacagcgc gaaggcggaa aaataaaaat aagtcgggga aatcagtccg
    12661 aaaccgtaat acatcagcgt cggaatcgcg ccgctcatca tcagcgcgct gaccatcagc
    12721 ccgatgaaga aaaacaggta aatcgcgccc ataccctgat tcaacgcgcc tatcatgccc
    12781 tgctgcatat cgttgtattt caaaccgcgc gccaagccgt acaaaatcag cacgacgatg
    12841 gcggcaataa tggacatatg cggcagccac tccaatgaaa tgatggtata gcccatcgcg
    12901 gcaatcagag cgacgacgac ggcaagtgcc tcaccgcgcg gcatatcgag taaggatttg
    12961 aaagcgaaca tgattctttg cccgatttga tataaagtta acggattcta acacaccata
    13021 ccgaatccgc ccgacgtttt cagacggcat tttagcgttt tacaaaagcc ggacagaaac
    13081 aaaacggcaa ttttctcaat ttaaattaat ttttttgaaa tataattttt tatttttttg
    13141 tttgttgaca tataagactt ttatggattg aagtattaaa acctcggcac acacgccgca
    13201 aacaccggta cgcaaaaatg ccgtctgaaa gatgttcaga cgacatttct caagaaaaag
    13261 attatttgtc cgccctatat ccgtacaccg caaaaaacac gatatacaga taacagatga
    13321 cggaaacgac aaacgaagac atcagggtgt aagtatctgc cacccagccc tgcacgacag
    13381 gaacgaccgc accgccgaca atcgcggtac acagtacacc ggaagccgcg ttggtaaatt
    13441 ttcccaatcc tttggttgcc aaagagaaaa tcgtcgggaa cataatcgag ttgaaaaaac
    13501 cgatggcaag cagcgaccac atcgccacat ccgcattgcc gctacccgtc gccatcgcga
    13561 cggcaagcag tacgaccgca gccgatgcgt taaacgccaa ataacggttg ggcgcgaatt
    13621 tcgccatcac cgccgaaccg aggaaacgtc cgaccatcgc gccgccccaa tagaacgaca
    13681 ggtaatgcgc ggcagaagca tgatccagcc ctttcagata acccaatacg ttgaccatca
    13741 acgaaccgat agacacctcc gcgccgacat agcagaaaat gcctgccgta ccgaacacga
    13801 gatgtttgta ttgccatacg ctggttttgc cgtcgtggtt gtgcgcgctt tcctcggcgg
    13861 caattttgcg cgcgtcgggc agccggatca ttttcacgaa aacggcaagg ataatcagca
    13921 gccccgccag tcccaaatag ggaatctgta cggaagaaat ctgttccgcc ttgctgacgg
    13981 tttgggttgc gtccgccaga atcaggaacg cgccgatttg cggcgcaatg gtcgtaccca
    14041 aagcgttaaa cgcctgaacc agcgtcagtg ttgccgattc cttgccgggt ttcgccagca
    14101 gggtaacata aggattaccg gcgacctgaa gcagcgttac gccggaagcc aaaataaaca
    14161 acgcgcccaa aaataccgcg taagaatggc tacccgcagc aggataaaac agcaggcatc
    14221 cgaccgccgt cagcagaaag ccgccgataa cgccgttttt gtagccgact ttgccgacaa
    14281 aagcccccat cgggatggac atcaccgcat aggcggtaaa gaaacagaat tggatcagca
    14341 tcgcctgaac gtaagacagg tcgaaaattt ctttcaaatg agggataagg atgtcgttca
    14401 tgcaggtaat aaaacccatc atgaagaaca gcgtggtcaa gacgaccaat ggggacgtat
    14461 gattgttttg tgattgcgca gacatgattt gcctttcgtg gtgtcggaat attacccgtt
    14521 tcaagaaaat agaaattgac atttcttaac ggcaatgcaa atcatactga tatttgaaca
    14581 ttttgcaaac tttatcggat gcaggcaatg cgccgatctg aaattcaagc agcagaaatt
    14641 aatattttac agttgcttgc atagatttta atagatttta aaaaattccg gcggacttgc
    14701 aaaaaccgtt cccaataaaa tgaaaatccg ccgccgtatc cggaaaattc ggaaatgccg
    14761 catcgtggca tcaaaaccgg agcggaaaga atgtcgtatg cccgacaaaa aaggggcata
    14821 cggctgtgcc gtattttttg caccgtcctc cgcccttcgg cgggggcgtg aaaatatagt
    14881 ggattaacaa aaatcaggac aaggcgac
//
LOCUS       DAXHNE010000035        14455 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.035, whole genome shotgun sequence.
ACCESSION   DAXHNE010000035 DAXHNE010000000
VERSION     DAXHNE010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14455
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.035"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(5..1006)
                     /locus_tag="ACFM4Z_000831"
     CDS             complement(5..1006)
                     /locus_tag="ACFM4Z_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFC6020896.1"
                     /translation="MIDRQTNEPKQKNRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRERLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEAYEPPAHWEWIRKIRDTVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPLARLKQWLGMMKKEFAAAQNLFDRVRTVKDADEVRNILAEFEREMNT"
     gene            complement(1187..5020)
                     /locus_tag="ACFM4Z_000832"
     CDS             complement(1187..5020)
                     /locus_tag="ACFM4Z_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HFC6020897.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAIPLDANSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWNMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGINIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITSVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHESVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKTRWEWLLANLDTPLADYKARYGAA
                     VHAAPEAKNNESCFIAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLNGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSNLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLIEAFLYEEQT
                     RRGVSIKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     AMGMAFLNATGPKTIKALRAAMIQIGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKNVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYADDNNMPADYIVIEMAKYILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5281..5377
                     /gene="ffs"
                     /locus_tag="ACFM4Z_000833"
     ncRNA           5281..5377
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACFM4Z_000833"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5544..7235
                     /gene="dld"
                     /locus_tag="ACFM4Z_000834"
     CDS             5544..7235
                     /gene="dld"
                     /locus_tag="ACFM4Z_000834"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFC6020898.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQLDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKVMQFVSKFLPDHLPKSMRDYRNKYEHHLILKMGG
                     KGVDEARAFLKEYFSHHGGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPSEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            7367..7474
                     /locus_tag="ACFM4Z_000835"
                     /pseudo
     CDS             7367..7474
                     /locus_tag="ACFM4Z_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7530..7901
                     /locus_tag="ACFM4Z_000836"
     CDS             7530..7901
                     /locus_tag="ACFM4Z_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFC6020899.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            8056..8604
                     /locus_tag="ACFM4Z_000837"
     CDS             8056..8604
                     /locus_tag="ACFM4Z_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC6020900.1"
                     /translation="MARLTVHTLETAPEAAKACVEAVLQNNGFIPNLIGVLANAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLAQTEINPELQAYA"
     gene            8740..9831
                     /locus_tag="ACFM4Z_000838"
     CDS             8740..9831
                     /locus_tag="ACFM4Z_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC6020901.1"
                     /translation="MNAQTLLTNVAELVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNQAVKDK
                     YLADILQGKVLAGTGMSNTVKHLVGIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQREGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANVVNAEVNSYLDDGYDSLKARLD
                     GAWAETERLADLAWDGAPDNLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
     gene            10467..10625
                     /locus_tag="ACFM4Z_000839"
     CDS             10467..10625
                     /locus_tag="ACFM4Z_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HFC6020902.1"
                     /translation="MCGPCGWIYDEEHGDPEHGIAPGTKFEDIPDDWKCPECGVGKED
                     FYLLDFVI"
     gene            11811..13607
                     /locus_tag="ACFM4Z_000840"
     CDS             11811..13607
                     /locus_tag="ACFM4Z_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFC6020903.1"
                     /translation="MNKIYRIIWNSALNAWVAVSELTRNHTKRASATVATAVLATLLF
                     ATVQASTTDDDDLYLEPVQRTAPVLSFHADSEGTGEKEVIGNTNLGIYFDEKRVLKAG
                     TITLKAGDNLKIKQNTDENTDENTNASSFTYSLKKDLTDLTSVETEKLSFGANGKKVN
                     ITSDTKGLNFAKETAGTNGDTTVHLNGIGSTLTDTLAGSSASHVDAGNQSTHYTRAAS
                     IKDVLNAGWNIKGVKTGSTTGQSENVDFVRTYDTVEFLSADTKTTTVNVESKDNGKRT
                     EVKIGAKTSVIKEKDGKLVTGKGKGENGSSTDESEGLVTAKEVIDAVNKAGWRMKTTT
                     ANGQTGQADKFETVTSGTNVTFASGKGTTATVSKDDQGNITVMYDVNVGDALNVNQLQ
                     NSGWNLDSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEITRNGKNIDIATSMT
                     PQFSSVSLGAGADAPTLSVDDKGALNVGSKDANKPVRITNVAPGVKEGDVTNVAQLKG
                     VAQNLNNRIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGYS
                     SISDGGNWIIKGTASGNSRGHFGASASVGYQW"
     gene            13762..>14455
                     /locus_tag="ACFM4Z_000841"
     CDS             13762..>14455
                     /locus_tag="ACFM4Z_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFC6020904.1"
                     /translation="MIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKNRYLRDHRGRPAY
                     PLLSIFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYSTLCRYRNRLAQ
                     DNTLSELLELINRQLTEKGLKIEKASAAVVDATIIQTAGSKQRQAIEVDEEGQISGQT
                     TPSKDSDARWIKKNGLYKLGYKQHTRTDAEGYIEKLHITPANAHECKHLSPLLEGLPK
                     GTTVYADKGYDSA"
BASE COUNT         3645 a         3951 c         3741 g         3118 t
ORIGIN      
        1 atattcaagt attcatttct cgctcaaatt cagccaagat gttccgaact tcgtccgcat
       61 ccttaaccgt tcggacgcgg tcgaacagat tttgtgctgc tgcaaattct ttcttcatca
      121 tacccagcca ctgtttcagc cgcgccagcg gatatttgtt gtttgcctct tttgtcaggc
      181 acagctcgaa aaactgccgt atccatttgg aaacttcggc aaaatccgtg tctttgaccg
      241 gcccgccgtt ctcgtattgc ttgatttgcc gcgccaaatc ggggcggatg accgcgccgc
      301 gaccgagcat cacgctgttg cagccgctga ttgttttgat gccgatatag tcctgcaggc
      361 tgaaaacgtc gccgttggcg gtaacgggaa tattgacggt atcgcggatt ttgcgtatcc
      421 attcccaatg cgccggcggt tcgtaagcct cggctttggt acgcgcgtgt acggtcagtc
      481 cgcatgcgcc cccttcggca atcgcacagg cgcattccaa agcccggctt ttgtcttcgt
      541 aaccgagccg catttttgcg gtgagcggaa tatgtgcggg caaacgttcg cgcagcgttt
      601 tgacgatgtg gaatatcagt tccggctctt ttaaaaggat tgcgccgcct ttgtgtttgt
      661 tgacggtggg cgcggggcag ccgaagttca aatcgatttt gttcgcaccg aagcggacgg
      721 cttccagcgc attcgccgcc atattgtccg catcgctgcc caaaagttgg acggtgcagg
      781 gcgtgccgga aaacgttttg tttccgttgg cgatttcggg gacatatttt aaccatatgg
      841 atcgggaatg cacggtatgg gtaatgcgta caaattcgct gacgcattcg tcgtagccgc
      901 caatacgcgt cagcaggtcg cgcatcacgt catcgaccag accctgcata ggggcaagga
      961 tgattcggtt tttttgtttg ggttcgtttg tttgtctgtc aatcatgatt tgggtaggta
     1021 tagtggatta aatttaaacc agtacagcgt tgcctcgcct tgccgtacta tttgtactgt
     1081 ctgcggcttc gtcgccttgt tctgattttt gttaatccac tatatttggt tcaaacaatg
     1141 ccgtctgaag gctttcggcg ttcagacggc atttccgtgt ccgttgttac agcaacactt
     1201 tctctacacc gccgttgttg gcttttttta caaactcatc cagccagttt tcgccgagga
     1261 tgtatttcgc catttcgatg acgatgtagt cggcaggcat attgttgtcg tcggcgtagc
     1321 ggctcaagcc ttgcaggcag gcggggcagg aggtcagcat tttgacgggt tcgccctgcg
     1381 gcagctcttt gaggtttttc tcgatttcct cttgtttgcg gaacttgacc tgagtggcga
     1441 tgtctggccg tttgacggca aacataccgg attcgccgca gcagcggtcg cttaaaacga
     1501 ctttctgccc catcaggctg ctggccattt gggtggcgtt catggttttg atgggggtat
     1561 ggcaggggtc gtggtagagg tattgctgac cttttacgcc gtcgagtttc acgccttttt
     1621 cgagcaggta ttcgtggatg tcgatgattc ggcagccggg gaagatttct tcaaagcggt
     1681 atttttcgag ctggtcgtaa caagtgccgc aactgacgac gacggttttg atgtcgaggt
     1741 agttgagggt gttcgccata cggtggaaag ccacgcggtt gttggtgctc atttcttcgg
     1801 cttttgcctt attgccgcct gcgtcttgcg gatagccgca acacatatag ccgggcggca
     1861 ggacggtttg tacgccgacg tgccagagca tggcttgaac ggcaagtccg atttggctga
     1921 acagacgctc agagccgcaa cccgggaagt agaacacggc ttcggcatct tcgggcgcgg
     1981 cggggttgcg gatgatgggg atgcttttgc cgtcttcgat gcccaataag gagcgcggtg
     2041 ttttggcggg tacgtttttg ggcagtgggc ggttgatgaa atggataacc tgttctttaa
     2101 tcggggcttt gccgacggtt gccttgggtt cggctttttg ctttttcgtg ccgattggaa
     2161 gaagtttgcc gattttgtag gcgaaattct gcgctgggaa gccgatctgt atcatggcgg
     2221 cgcgaagggc tttgatggtt ttcgggccgg tggcgttcaa aaacgccata cccatagctg
     2281 cggcaggcgc aaatcgtttg tggccggaat cggcaagata gttgcggacg gctacggtaa
     2341 cgtcgccgaa gtcgatgttg acggggcagg gtttgacgca gcggtggcac acggtgcagt
     2401 ggtcgccgat gtccatgagt tcttcaaagt gtttgatgga aacgccgcgg cgggtttgtt
     2461 cttcgtataa gaaggcttcg atcaataagc ccacgccgag gattttgttg cgggggctgt
     2521 acagcaggtt ggcacgcgga acgtgagtag agcagacggg tttgcatttg ccgcagcgca
     2581 ggcagtcttt gacggaatcg gcaatcgtgc cgaggtttga tttttccata atcagcgatt
     2641 ccgcgcccaa cagctcgaag gacggcgtgt aggcgttgcg taagtccgag cctttcatca
     2701 gtttgtgacg gttgaaggtg tgtttcggat cgacttggtt tttgtagtcc caaaacggct
     2761 gcatttcttc gtcgcttaaa aattcgagct tggtaatgcc gatgccgtgt tcgccggaaa
     2821 tcacgccgtt aagcgaacgg gcgattttca taatgcgttc gactgagcgg tatgccgtct
     2881 gaagcatttc ggcatcgtct gagttaaccg gaatattggt gtgaacgtta ccgtcgccgg
     2941 cgtgcatatg cagggcgaca aagacgcggc tgcgtacggt ttttgcgtgg atttttccca
     3001 agccttggat aattttggtg tcggttttgc cgctgaagat ttcagaaagc ggtttcatta
     3061 cgtccgcttt gacagacacg cgcaggcgga aatcgcggaa ggcaataaag cagctttcat
     3121 tgtttttggc ttcgggtgcg gcgtggacgg ctgcgccgta gcgggctttg tagtcggcaa
     3181 gcggcgtgtc aagattggcg agcagccatt cccaacgcgt tttgacggcg gaaacgtggg
     3241 ccagggcgtg tttgccgcgt tcgcccaaca gttcggcggt cggcaggtca gtgcccattt
     3301 tgtcgatggg gagtttgccc gaaagatatt gctccaaggc ggcacagagt ttgagcttgt
     3361 tttggatgga aagctcaatg ttgatgcgtt cgatgccgtc cgaatactcg ccgagccttt
     3421 cgagcgggat gaccacgtct tcgttgattt taaaggcgtt ggtatgtttg gcgatggcgg
     3481 cggtgcggct gcggtcgagc cagaaggttt tgcgggcttc gggcgatacg gcgataaagc
     3541 cttcgccgtc gcgggcgcgt gcgagttcac agatgtgttc ggcggctgcc tctacggcgg
     3601 cttcgtcgtc tgaaaccacg tctgccagca aaaccatttt cggtcgtccc ttgcccgccg
     3661 ctttggtggc gtagccgacg gcgcggacat aacgccagtc caaatgttcc aaacccgcca
     3721 gccgcacgct ttcatgggca agcaaaaagt cgcggatttc gacaatagat ggcgtggcgg
     3781 tggcgaccgt accgaaaaac tccatacaca cggtgcgcgt gtatttcggc attttatgca
     3841 acacgaaggc aacgctggtg atgatgccgt ctgtaccttc tttttgcacg ccgggcaggc
     3901 cgctcaagaa tttgtcggta acgtctttgc ccaaaccgac tttgcggaat ttgtggccgg
     3961 ggatttccaa gcgttcggtt ttaacgatat tgatgccgtc tgaatccagc gtgtgaacgt
     4021 cgaacacggc ggtttcttcg tcgtggattt tgccgaaatt gtggcgcacg cgctcgatac
     4081 gcagccattc gccttgaggg ttaaccatgt tccagtaggc gaggttgtcc aacgccgtcc
     4141 cccacagcac ggcttttttg ccgcccgcgt tcatcgccac attaccgccc acgcatgacg
     4201 cgtcggcaga agtcggatcg acggcgaaca ccaaacctgc ctgatgcgcg gtttcttcca
     4261 cccgccgcgt aaccacgccc gcgccgcacc ggataatcgg atgcttgccg tccaagcctg
     4321 ccagctcaac gtattcaaca ccacgatgct tgtcgagttt ttcggtattg atgactgcgc
     4381 tgtttgcgtc caaaggaatc gcgccgccgg tataacccgt gccgccgccg cgcggaataa
     4441 tgaccaaatc cagctcgatt aaggcgcgca ccaaaggcgc gatttcagcc tccgtgtcgg
     4501 gattgacgac gacaaacgga tactccacgc gccagtcggt tgcatcggta acgtgcgtta
     4561 cccgcgccag cccgtcgaac ataatattgt gcggcttggt gattttgctc aaacgctcca
     4621 aaatctgccg ccgtttttgg ctggtttcat caaaactgct atcaaaacgc tcgactgctt
     4681 tttctgctgc ggcaaccaac acatccactt gccgattatc gtcgcggcgt ttgcggattt
     4741 catttaagcg gtggcgcatt tcacgtacca gcgcggcgcg gcgttttggg tgctccagca
     4801 agtcatcgac cagatacgga ttgcgcacga ccacccaaat atcacccaac acttcaaaca
     4861 gcatccgcgc cgaacgcccg gttttgcgtt gaccgcgcag gtcttgcagg atttgccacg
     4921 cttcgtcgcc caataatcgg atgacgattt cgcggtcggt gtaggaagta tagttgtagg
     4981 ggatttcccg aatacgctga ggggcggtag tcgtggtcat ggtgtgtccg tgtttggcag
     5041 tttttatttg aatgccgtct gaaaagtcgg tttcaggcga gtttgaaaag gcgataatgt
     5101 agttcaattt ttgatacttt tcaatgccga aacaggaaat acccttatag aaacaaaata
     5161 acgctttgag aaattgttga caatattatc aaatataaat tttaggtggt gtttccggcg
     5221 tatgaccgta tcaaaacggt tgagtttttc agaaaacccg ttataatgcc gtttctctga
     5281 ggcgggtctc cccgcatggc aaatcggaac accgggtcag gggcggaagc cagcagccca
     5341 ctccaatgcg ccagtgccgg gggtttggcc cgccgcccta tttgaaacgc cggagcttcg
     5401 atgttccggc gttcccgtat cgcaccgatg ccgtctgaaa gccgttcaga cggcattttc
     5461 tttactttgc cataacgtat aactacaatg tgaaaacaat tccaatgtgc aatgcacctg
     5521 ccgacaattt taaggaaact gccatgagcg catcacaatt actgtcaaga ctgacccaaa
     5581 ccgtaggcga aaaatacatc atcaccgacc ccgcgaaaac cgaacaatac cgccaaggtt
     5641 accgcttcgg cgagggtaag gcgttggcgg tggttcgccc cggaagcatt ctggaaatgt
     5701 ggaaaattct gcaggcgtgc gtcgaagcag acgtgattgt gattacgcag gcggcgaata
     5761 ccggtttgac gggcggctcg acccccgacg gcaacgatta cgaccgcgac atcgtgattg
     5821 tgaacaccat gcggatgaac atcatccaaa ccatcaacaa caacgaacaa gtcgtctgcc
     5881 tgcccggctc gaccctgaac cagcttgaat tgctgctgaa acctttgggg cgcgaaccgc
     5941 attcggtcat cggctcatcc tgtatcggcg cgtccgtctt gggcggcgtg tgcaacaact
     6001 ctggcggcgc gttggtacag cgcggcccgg cctacaccga aatggcgttg ttcgcccaaa
     6061 tcaacgaaga aggcaggttg gaactggtca accacttggg catagacttg ggcgatacgc
     6121 cggaagaaat cctgaccaac ctgcaaggtc atcattatca aaacaaagac atcaaacaag
     6181 acgcgggcaa aggacacgac cacgcctatt gcgaacacgt ccgccaattg gacgaaccga
     6241 ccgccgcgcg tttcaacgcc gaccccgcgc gccattacga agcctcgggc tgcgcgggca
     6301 aactgatggt gttcgccgtc cgtttggaca ccttcccgca agaaaaacaa accgccgtgt
     6361 tctacatcgg caccaacgac atcaacgagc tgaccgacat ccgccgcgcc gccttgggcg
     6421 aatttgaaag cctgcccgtt tccggcgaat acatccaccg ccacgctttc gatattgccg
     6481 acgtgtacgg caaagacacg ttttacgtca tcaaaaaatt cggtacgcac caactgccga
     6541 aattatttga tttgaaggca cgcgtggaca ggttcggcaa aaaagccagc ttcctgccca
     6601 agcatttttc cgacaaggtc atgcagttcg tcagcaaatt cctgcccgac cacctgccca
     6661 aatccatgcg cgattaccgc aataaatacg aacaccacct gattctgaaa atgggcggga
     6721 aaggcgtgga tgaggcgcgc gcattcttaa aagaatattt ttcacaccac ggcggcgcgt
     6781 ttttcgaatg caacgccgaa gaaacccaag ccgcgatgct gcaccgtttc gccgtcgcct
     6841 ccgccgccat ccgctaccgc gccgtgcatg atgacgaagt ggaagacttg gtcgcgctcg
     6901 acatcgccct gcgccgcgac gatcgcgact ggtttgaaaa actgccgtcg gaaatcgaca
     6961 ataaaatcat ccataaactc tactatggac atttcatgtg ccacgttttc catcaggatt
     7021 acatcatcaa aaaaggcaac gactgcatgg cgttggaaca cgaaatgctg catcttttag
     7081 accaacgcgg cgcgcaatat cccgccgaac acaacgtcgg tcatttgtat gaagccaaac
     7141 ccgcgctcaa acagttttac cgcaaacttg acccgaccaa cagcttcaac ccgggcgtag
     7201 gcaaaaccag caaaaagaaa aactgggcgg aataagcgcg tccgctttga aggcaggcaa
     7261 tgccgtctga aggcaaaacg ccgttcagac ggcattgtcg ggtttgaagt tccccgtcgg
     7321 gtaatcgctt tcaaaccgta tgccgacaca cgggcgcgcc cctcccaaat atagtggatt
     7381 aaatttaaat caggacaagg caacgaagcc gcagacagta cagatagtac ggcaaggcga
     7441 ggtaacgccg tactggttta aatttaatcc actataaagt ctgatcctac cttcccaaag
     7501 ggcagggttt caaccgaaaa ggaaacacga tgaaaccata caaaatctac acccatcccg
     7561 ccctgccgcc ccaagccgtc aaacaaggct ggtcgtggcc gggccttttg ttcggcacgc
     7621 tgtgggcgtg tttcaaaaga atgtggggtt tggggctggg gctgacgggc gcgatattcg
     7681 tgctggctgt gtttgcccaa ctggtttacg gcgacacgcc cgccacagac tcggcgttta
     7741 acgtattggg cttggcggtt tccgtctggt tcggcgcaaa gggcaacagc ctttatgcac
     7801 gccacctttt atcgcgcggc tataccgaat tgcccgaaac ggttgaggca gccaatcccc
     7861 aagccgcatt ggcgcaatat ttcgggcgcg ggggccgata agcgcgtttt cttttccatc
     7921 atgccgtctg aaaaatgttc agacggcatt tcttataacc ttttgtttgc agcacataac
     7981 caatcagtct ttcctattcc gttccgaaat gcataccatg cgcccgtccc caatatttgc
     8041 gaacaaggaa agaaaatggc acgtttaacc gtacacaccc ttgaaaccgc ccccgaagcc
     8101 gccaaagcgt gcgtagaggc ggtacttcaa aacaacggct tcatccccaa ccttatcggc
     8161 gtattggcaa acgcccccga agccttggcg ttttaccaag aagtcggcaa gctcaacgcc
     8221 gccaacagcc tgactgccgg cgaagtcgaa gtcatccaga tcatcgccgc ccgcaccaac
     8281 caatgcggct tctgcgtggc agggcatacc aaactggcaa ccctgaaaaa actcctttcc
     8341 gaacaatccg tcaaagccgc gcgcgctttg gcggcaggcg aatttgacga tgccaaactc
     8401 ggcgcgctcg ccgccttcac ccaagccgtg atggcgaaaa aaggcgcggt atccgacgag
     8461 gaactcaaag cattttttga tgcaggctac aatcagcagc aggcggtcga agtcgtgatg
     8521 ggcgtagcct tggcaaccct gtgcaactac gtcaacaacc tcgcccaaac cgaaatcaac
     8581 cccgaattgc aggcatacgc ctaagcaaac aaaaccgcct gaatgcaggc ggtttttcaa
     8641 aaacaccccc tcaaacaaaa acaagccgcc caagcggcag gggaagcagc cgcgcatcag
     8701 gcgcgccccc gattccctcg caaaccttaa ggaacaaaga tgaacgccca aaccctactt
     8761 accaacgttg ccgaactcgt caaaaccaag ctcaaaccca tagtggacga catcgaccgc
     8821 aaaggatact accccgaagc atttatgcgc gaactcggcg caatcggcgg attcggcgca
     8881 gtcggtacag aagccgaagg cggcaacggc ttgggtttgg caacgcaaat cgctgttttg
     8941 cgcgaaatcg gcaaagaatg cggcgcaacc tccttcagcg cgtggtgtca ggcggcttgc
     9001 gcgtggtatc tgcaccaaac gcccaaccaa gccgtcaaag acaaatacct cgccgacatc
     9061 ctgcaaggca aagtattggc aggcactgga atgtccaata ccgtcaaaca ccttgtcggc
     9121 attgaaaaac acaaccttca agccgagcgc gtggagggcg gctacacagt caacggcgcg
     9181 ttgccgtggg tgtccaacat cggcgaagac cacatctggg cgaataccgc ccaaatcggc
     9241 ggcggctacg ttatgttcat cacaggcggg caaagggaag gcgtaagcct gcaaaactgc
     9301 cccgaatttt gcgccctcga aggcacgcgc accttcagcc tgaacttcaa agacgtattt
     9361 atccccgacg aagacatcat cgccgcgccc gaacagtttg ccgactacat ccaaagcatc
     9421 aaagcaggct tcatcttgct gcaaatcggc atcggcgcgg gcgtgattga cggcagcctc
     9481 ggcatcatcc gcctcgccaa cgtcgtcaac gccgaagtca acagctatct cgacgacggc
     9541 tacgacagcc tcaaagcaag gttggacggc gcgtgggcag aaaccgaacg gctcgccgac
     9601 ttggcgtggg acggcgcgcc cgataacctc gccaccctca agctgcgcga agccgccgcc
     9661 gtactggcac ttgccgccgc ccaatccgcc gccctgcatt ccggcgcaaa aggctacctg
     9721 atgcgcagtc ccgcccaaag gcgcgtccgc gaagcaatgt ttgtcgccat cgtaaccccc
     9781 gcgatcaaac acctgcgcaa agaaatagcg gcaatcgaag ccgcaaaata aacaggcgga
     9841 aaaaacagaa acccccaccg cgtcattccc gcgaaagcgg gaatccagcc cccaacgcaa
     9901 caggaatcca tcggaaaaaa cagaaacccc caaaccgtca ttcccgcaaa agcgggaatc
     9961 cagcccccta acgcaacagg aatccatcga aaaaaacaga aaccctcaaa ccgtcattcc
    10021 cgcaaaagcg ggaatccagc ccccaacgta acaggaatcc atcggaaaaa acagaaaccc
    10081 tcaaaccgtc attcccgcaa aagcgggaat ccagccccct aacgcaacag gaatccatcg
    10141 aaaaaaccga aacctccaaa ccgtcattcc cacggaagtg ggaatccagt aaccgaaaaa
    10201 ccacaggaat ctatcgaaaa aaaacccaac ccccaaaaaa accgggcgga tacctcacca
    10261 tcctcccaaa ccctgattta tagcggatga acaaaaatca gggcaagacg gcgaaaccgc
    10321 agacagtaca aatagtacga aaccgattca cttggtgctt gagcacctta gagaatcgtt
    10381 ctctttgagc taaggcgagg caacgccgta ccggtttttg ttaacccact atacaaaaaa
    10441 caaggaaaca caaatggcgc aataatatgt gcggcccctg cggctggatt tacgatgaag
    10501 aacacggcga ccccgaacac ggcatcgccc ccgggacaaa gtttgaagac atccccgacg
    10561 actggaaatg ccccgaatgc ggcgtgggca aagaagattt ctacctgttg gatttcgtga
    10621 tatagcggca gccgcaccct tagggaaaac cccgtcatcc ggcgttccgc ctgcggcggc
    10681 agggaacacc gttttatagt ggattaacaa aaatcatgac aaggcgacga agccgcagac
    10741 agtacaaata gtacggaacc aattcacttg ttaaagaatc gttctctttg agctaaggcg
    10801 aggcaacgcc gtactggttt ttgttaatcc actatatccg tttgcgcccg ccaagccgtg
    10861 ttgcacagat gcgacacggc tttttccttt tcgtcgtccg aatgtaaatt ttcggcaaaa
    10921 aacgtcaaaa atcacccccc ccccgcattt gcgtaaaata aagtgtaatc cctttgatca
    10981 taagggaatg ttgacaatta gatcgattct cctaggctga ctttcgcaat aaatggggtt
    11041 gtcaataatt gtgtatggtc gtttgccgtc cggcggccgt cattcccgcg caggcgggaa
    11101 tccagcaaca caacgcagca ggactttatc ggaaaaaacc gaaaccccac cgaccgtcat
    11161 tcccgcaaaa gcgggaatcc aaaaacgcaa cgcaacagga atttatcgga aaaaaccgaa
    11221 accccaccgc cgtcattccc gcaaaagcgg gaatccagca accgaaaaac cacaggaatc
    11281 tatcagcaaa aacagaaacc cccaccgccg tcattcccgc gcaggcggga atccagcaac
    11341 acaacgcggc aggactttat cggaaaaaac agaaacccca ccgccgtcat tcccgcaaaa
    11401 gcgggaatcc agacccgtcg gcacggaaac ttaccggata aaacagtttc cttagattcc
    11461 acgtcctaga ttcccgcttt cgcgggaatg acgagatttt agattatggg aatttatcag
    11521 gaatgattga atccatagaa aaaccacagg aatctatcag aaaaaacaga aaccccaccg
    11581 ccgtcattcc cgcaaaagcg ggaatccaga cccgtcggca cggaaactta ccgaataaaa
    11641 cagtttcctt agattccacg tcccagattc ccgccttcgc gggaatgacg agattttaag
    11701 ttgggggaat ttatcagaaa acccccaacc cccaaaaacc gggcggatgc cgcaccatcc
    11761 gcccccaaac cccgatttaa ccattcaaac aaaccaaaag aaaaaacaaa atgaacaaaa
    11821 tataccgcat catttggaat agtgccctca atgcctgggt cgccgtatcc gagctcacac
    11881 gcaaccacac caaacgcgcc tccgcaaccg tggcgaccgc cgtattggcg acactgttgt
    11941 ttgcaacggt tcaggcgagt actaccgatg acgacgattt atatttagaa cccgtacaac
    12001 gcactgctcc cgtgttgagc ttccatgccg attcagaagg cacgggagaa aaagaagtta
    12061 taggaaatac aaatttggga atatatttcg acgagaaaag agtactaaaa gccggaacaa
    12121 tcaccctcaa agccggcgac aacctgaaaa tcaaacaaaa caccgatgaa aacaccgatg
    12181 aaaacaccaa tgccagtagc ttcacctact cgctgaaaaa agacctcaca gatctgacca
    12241 gtgttgaaac tgaaaaatta tcgtttggcg caaacggcaa gaaagtcaac atcacaagcg
    12301 acaccaaagg cttgaatttc gcgaaagaaa cggctgggac gaacggcgac accacggttc
    12361 atctgaacgg tatcggttcg actttgaccg atacgcttgc gggttcttct gcttctcacg
    12421 ttgatgcggg taaccaaagt acacattaca ctcgtgcagc aagtattaag gatgtgttga
    12481 atgcgggttg gaatattaag ggtgttaaaa ctggctcaac aactggtcaa tcagaaaatg
    12541 tcgatttcgt ccgcacttac gacacagtcg agttcttgag cgcagatacg aaaacaacga
    12601 ctgttaatgt ggaaagcaaa gacaacggca agagaaccga agttaaaatc ggtgcgaaga
    12661 cttctgttat taaagaaaaa gacggtaagt tggttactgg taaaggcaaa ggcgagaatg
    12721 gttcttctac agacgaaagc gaaggcttag tgactgcaaa agaagtgatt gatgcagtaa
    12781 acaaggctgg ttggagaatg aaaacaacaa ccgctaatgg tcaaacaggt caagctgaca
    12841 agtttgaaac cgttacatca ggcacaaatg taacctttgc tagtggtaaa ggtacaactg
    12901 cgactgtaag taaagatgat caaggcaaca tcactgttat gtatgatgta aatgtcggcg
    12961 atgccctaaa cgtcaatcag ctgcaaaaca gcggttggaa tttggattcc aaagcggttg
    13021 caggttcttc gggcaaagtc atcagcggca atgtttcgcc gagcaaggga aagatggatg
    13081 aaaccgtcaa cattaatgcc ggcaacaaca tcgagattac ccgcaacggt aaaaatatcg
    13141 acatcgccac ttcgatgacc ccgcagtttt ccagcgtttc gctcggcgcg ggggcggatg
    13201 cgcccacttt gagcgtggat gacaagggcg cgttgaatgt cggcagcaag gatgccaaca
    13261 aacccgtccg cattaccaat gtcgccccgg gcgttaaaga gggggatgtt acaaacgtcg
    13321 cacaacttaa aggcgtggcg caaaacttga acaaccgcat cgacaatgtg gacggcaacg
    13381 cgcgtgcggg catcgcccaa gcgattgcaa ccgcaggtct ggttcaggcg tatctgcccg
    13441 gcaagagtat gatggcgatc ggcggcggca cttatcgcgg cgaagccggt tacgccatcg
    13501 gctactccag tatttccgac ggcggaaatt ggattatcaa aggcacggct tccggcaatt
    13561 cgcgcggtca tttcggtgct tccgcatctg tcggttatca gtggtaaggg ctttttcgcc
    13621 tgtctgctgt ttgggatagg cggaaggttt gaagggaagg gcggcgattt gccgcctgag
    13681 acctttgcaa aattccccaa aatcccctaa attcccacca agacatttag gagcaccttc
    13741 tttcaacaaa ccgcgcaagc catgattgcc aaacacatcg accgtttccc gctattgaag
    13801 ttggatcagg taattgattg gcagccgatc gagcagtacc tgaaccgtca aaaaaaccgt
    13861 tacctccgag accaccgcgg ccgtcccgcc tacccgctgc tgtccatatt caaagccgtc
    13921 ctgctcggac aatggcacag tctctccgat cccgaactcg aacacagcct cattacccgc
    13981 atcgatttca acctgttttg ccgtttcgac gaactgagca tccccgatta cagcacctta
    14041 tgccgctacc gcaaccggct ggcgcaagac aataccctgt ctgaactgtt ggaactgatt
    14101 aaccgccaac tgaccgaaaa aggtttaaaa atagagaaag catccgctgc cgtcgttgac
    14161 gccaccatta ttcagaccgc cggcagcaaa cagcgtcagg ccatagaagt tgacgaagaa
    14221 ggacaaatca gcggtcaaac cacaccgagt aaggacagcg atgcccgttg gataaagaaa
    14281 aacggcctct acaaactcgg ttacaaacaa catacccgta ccgatgcaga aggctatatc
    14341 gagaaactgc acattacccc cgccaatgcc catgagtgca aacacctgtc gccgttgttg
    14401 gaaggactgc ccaaaggtac gaccgtctat gccgacaaag gctacgacag tgcgg
//
LOCUS       DAXHNE010000036        14438 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.036, whole genome shotgun sequence.
ACCESSION   DAXHNE010000036 DAXHNE010000000
VERSION     DAXHNE010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14438
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.036"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(107..451)
                     /locus_tag="ACFM4Z_000842"
     CDS             complement(107..451)
                     /locus_tag="ACFM4Z_000842"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFC6020905.1"
                     /translation="MNKQYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYAEK
                     FYFHDEYQQLDL"
     gene            complement(444..638)
                     /locus_tag="ACFM4Z_000843"
     CDS             complement(444..638)
                     /locus_tag="ACFM4Z_000843"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6020906.1"
                     /translation="MRKVFHYEQNRALTVRELAALQSFPDNFIFCGSKIAQQQQVGNA
                     VPPLLAKAIAESILKMSENE"
     gene            complement(616..1446)
                     /locus_tag="ACFM4Z_000844"
     CDS             complement(616..1446)
                     /locus_tag="ACFM4Z_000844"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6020907.1"
                     /translation="MHTPLTYIDLFSGAGGLSLGFEQAGFQQLLSVEMESDYCQTYRT
                     NFPHHQLLQKDLTTLTEQDLINCLNGQAVDLIIGGPPCQGFSMAGKIGRTFTDDPRNH
                     LFKEFVRIVKIVQPYFFVMENVARLYTHNSGKTRIEIIQAFQNIGYSVECKILSAADF
                     GVPQIRSRVIFIGRRDKGKISFPEPLQISHQTVGSAIGHFPKLAAGESNPHVANHEAM
                     NHSAQMLEKMAFVKNGGNRNDIPEPLRPKTGDIRKYIRYNSNKTSRLYYRRYAQSFSL
                     "
     gene            complement(1477..3750)
                     /locus_tag="ACFM4Z_000845"
     CDS             complement(1477..3750)
                     /locus_tag="ACFM4Z_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFC6020908.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDGLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQPLDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQNQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPTNSAMADAFAKLKR"
     gene            complement(3814..4464)
                     /locus_tag="ACFM4Z_000846"
     CDS             complement(3814..4464)
                     /locus_tag="ACFM4Z_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6020909.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            complement(4461..5258)
                     /locus_tag="ACFM4Z_000847"
     CDS             complement(4461..5258)
                     /locus_tag="ACFM4Z_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6020910.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLRADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGSDVITYENPWYIVLCNLVLLLFGLAMVSKFSKGVEPQ"
     gene            complement(5258..6421)
                     /gene="ctrB"
                     /locus_tag="ACFM4Z_000848"
     CDS             complement(5258..6421)
                     /gene="ctrB"
                     /locus_tag="ACFM4Z_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HFC6020911.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(6436..7611)
                     /gene="ctrA"
                     /locus_tag="ACFM4Z_000849"
     CDS             complement(6436..7611)
                     /gene="ctrA"
                     /locus_tag="ACFM4Z_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HFC6020912.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            7738..8871
                     /gene="neuC"
                     /locus_tag="ACFM4Z_000850"
     CDS             7738..8871
                     /gene="neuC"
                     /locus_tag="ACFM4Z_000850"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFC6020913.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHLDLELHLIVTGMHMMKT
                     YGRTCKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGATVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKYQSDKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            8875..9561
                     /gene="neuA"
                     /locus_tag="ACFM4Z_000851"
     CDS             8875..9561
                     /gene="neuA"
                     /locus_tag="ACFM4Z_000851"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HFC6020914.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            9562..10611
                     /gene="siaC"
                     /locus_tag="ACFM4Z_000852"
     CDS             9562..10611
                     /gene="siaC"
                     /locus_tag="ACFM4Z_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HFC6020915.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIE"
     gene            10695..12182
                     /locus_tag="ACFM4Z_000853"
     CDS             10695..12182
                     /locus_tag="ACFM4Z_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2,8-polysialyltransferase family protein"
                     /protein_id="HFC6020916.1"
                     /translation="MLKKIKKALFQPKKFFQDSMWLTTSPFYLTPPRNNLFVISNLGQ
                     LNQVQSLIKIQKLTNNLLVILYTSKNLKMPKLVHQSANKNLFESIYLFELPRSPNNIT
                     PKKLLYIYRSYKKILNIIQPAHLYMLSFTGHYSYLISIAKKKNITTHLIDEGTGTYAP
                     LLESFSYHPTKLERYLIGNNLNIKGYIDHFDILHVPFPEYAKKIFNAKKYNRFFAHAG
                     GISINNNIANLQKKYQISKNDYIFVSQRYPISDDLYYKSIVEILNSISLQIKGKIFIK
                     LHPKEMGNNYVMSLFLNMVEINPRLVVINEPPFLIESLIYLTNPKGIIGLASSSLIYT
                     PLLSPSTQCLSIGELIINLIQKYSMVENTEMIQEHLEIIKKFNFINILNDLNGVISNP
                     LFKTEETFETLLKSAEFAYKSKNYFQAIFYWQLASKNNITLLGHKALWYYNALYNVKQ
                     IYKMEYSDIFYIDNISVDFHSKDKLTWEKIKHYYYSADNRIGRDR"
     gene            complement(12678..13835)
                     /locus_tag="ACFM4Z_000854"
     CDS             complement(12678..13835)
                     /locus_tag="ACFM4Z_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6716 putative glycosyltransferase"
                     /protein_id="HFC6020917.1"
                     /translation="MKKVLLIASYDSFLNSGYAVAKEIKDAQIDIYIHKSRENILSNR
                     QLLESGIDKDQAIFFHIDDYFIKNMHQYYDAVILSVGNGLLKRFFKQNAQLNIASRPL
                     IITLFPGIVFGDQASILSRMGADIVLYNNKHDFRIAEEYKKQYKLSCQNILYGYPIFR
                     HASKGRHGEKIYFIDQVKIPFKKEERIYTLKKLIALAEKYPEKEFTILLRVADKDITV
                     HQDKHSYIELAKQFQLPSNLTIERKSTAQAFQEMGYCLSYSSTMLFEAECKGIPVGVV
                     ADLGFSKSYANQHFLGSGVLVYFDQIDFTSPKIADPDWLDCYATKKVITTDEFNKLLK
                     QVVPLQHDYQEYLSAVNSIESTKTIFLRKFKKLIRDPKKFFYDSKWLRKVI"
     gene            14012..>14438
                     /locus_tag="ACFM4Z_000855"
     CDS             14012..>14438
                     /locus_tag="ACFM4Z_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC6020918.1"
                     /translation="MKKILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMP"
BASE COUNT         4276 a         3274 c         2816 g         4072 t
ORIGIN      
        1 ggcgcatatc ttccgtatag ggcattttac gtatcatggc gcgacgcatt aaagtaaacg
       61 ccaaagcgcg tttatactgg ttatccagtt tccctatatt tttacgttac aaatcaagtt
      121 gttggtattc atcgtgaaaa tagaattttt cggcataacg ctgactcata aaaccggcat
      181 gcgggctgcc tgaaaaaatc attgcgacat catcgtcatt taatgtttca tggttgtttt
      241 ctattaatgc taaagcaatt tggtaattaa ttgccaaaat atcgttagta atcacacgat
      301 agccgcgttt tttggcttcg taggcaaagg aacagcctcc actaaataca tctgcaactg
      361 tatctacatc agacggaagc tggtcacaaa tccaggaagc tattttctct ttattaccga
      421 tatagttaat tttcggatat tgcttattca ttttcactca tttttaaaat actttcagca
      481 atagcttttg ccaataaagg cggtacggcg ttaccaacct gctgctgctg ggcaattttg
      541 ctgccgcaaa aaataaaatt atcagggaaa gattgtaagg cagctaattc acgaacggtt
      601 aacgcccgat tctgttcata gtgaaaaact ttgcgcatat ctcctgtaat acaaacggct
      661 ggttttgttg ctgttgtaac ggatgtattt acggatatca cctgttttcg gacgtaatgg
      721 ttcaggaata tcgttacggt tacctccatt tttaacaaat gccatttttt ctaacatttg
      781 tgccgaatga ttcatagctt catgatttgc aacgtgtgga ttgctttcgc cagcagccag
      841 ttttggaaaa tgtcctattg ctgatccaac agtctgatgg gaaatctgca aaggttcggg
      901 aaaggaaatt ttgcctttat ccctcctccc gataaatatc actcggctac gtatctgagg
      961 aacaccgaaa tcggctgcac tcagtatctt acattccacc gaataaccga tattctgaaa
     1021 tgcttgaata atctcaatac gtgttttacc tgaattgtgt gtatagagtc gcgctacatt
     1081 ttccataaca aaaaaatatg gttggacaat tttaactatt cggacaaact ctttaaataa
     1141 atggttgcgt gggtcatctg taaatgtccg tccaatcttt cctgccatac taaaaccttg
     1201 acaaggtggt cctccaataa tcaaatcaac tgcttgtccg ttaagacaat tgattaaatc
     1261 ttgttcggtt agtgtggtta aatctttttg cagtaattga tgatggggga agttggtacg
     1321 gtaagtctga caataatcag actccatttc aacagaaagc aattgttgga atccggcttg
     1381 ttcaaaaccc aaggataggc ctcctgctcc tgagaaaagg tcaatatagg taagtggtgt
     1441 gtgcataagt caaaatccat aaactcttcg attattttac cgcttcagct tcgcaaacgc
     1501 atccgccatc gccgaattgg tcggggcgcg gtcgttgcgt tggggtttgc ggccggctgt
     1561 ccgttcgcgg ctgcggtttt cagacggcat tttatgtttt gcgccgcccg gttcgtcatc
     1621 caagcgcatg gtcagcgcga tgcgtttgcg tgcagcatcg acttccagca ctttcacttt
     1681 caccacgtcg ccagctttca ccacttcgcg cgggtcttgg acgaacttgt tggacagggc
     1741 ggagatgtgc accaagccgt cctgatggac gccgatgtcc acgaacgcgc cgaagttggc
     1801 gacgttggaa accacgcctt cgagtatcat accgacttgc aagtcgctga tttcgtggat
     1861 accttcggca aacgatgccg tctgaaactc gccgcgcgga tcacggccgg gtttttccag
     1921 ttcggacagg atgtccaaaa tggtcggcag gccgaagcgt tcgtcggtga agtcggacgc
     1981 tttgatttgc ttcacgcgct cgcggttgcc gatgagttcg gcggcgctaa tgccttgttg
     2041 cgccagcatt ttggcgacga cgggataggc ttcggggtgg acggcgctcg cgtccaacgg
     2101 ctctttaccg ccgttaatcc gcaaaaagcc tgccgcctgc tcgaaggttt tttcgcccaa
     2161 acgcggtact ttcagcaatt ttttgcggct gtcgaacgcg ccgttttcat cgcggtaggc
     2221 aacgatgttt tgggcaaggg tttgattcaa gccggaaatc cgcgccaaga gcggggcgga
     2281 ggcggtattc acgtccacgc cgacggcgtt cacgcagtct tcgaccactg cgtccagcga
     2341 tttggcgagc tggttttggt tcacatcgtg ctgatactgg cccacgccga tggatttagg
     2401 gtcgattttg accaactcgg caagcgggtc ttgcagcctg cgggcgatgg acaccgcgcc
     2461 gcgcagggaa acgtccaagt cggggaactc gcgcgccgcc agttcggacg cggaataaat
     2521 cgacgcgccg gcttcggaca cgacgatttt atgcagcccc attttcgaca tttcacgcac
     2581 cagttcgccc gcgattttgt cggtttcacg gctggcggtg ccgttaccga tggcgatgag
     2641 cttcacgccg tgctgtttaa tcaggcgcga cagcgttgcc aacatattgt tttcttgatg
     2701 caaatagacg atgacggtat ccagcagctt gccggtgtcg tccaccacgg cgcatttcac
     2761 gccgttgcgg tagccggggt cgagacccag cgtggtcagc cgtccggcgg gcgcagcaag
     2821 cagcaagtct ttgagattgc gggcgaacac ggtaatcgcg tcggtgtcgg cggcttcttt
     2881 caaacgattg agagcttcaa gttccaacga caaaaagatt ttcgcgcgcc aagtcaggcg
     2941 cacggtgtcg cgcagccatt tgtggccgtc tgaaactttg aagcggcggg cgatgatttg
     3001 ctcgtattcg ctttgctgcg taatcggcgt gtcgtcgggc tgatatttga gcgcgatgtt
     3061 caacacgcct tcgttgcgtc cgcgcaaaac cgctagcgca cgatggctgg gcatcgcacg
     3121 aataggttcg cggtggtcga aataatcgct gaatttttcg ccttcggttt ctttgcctcc
     3181 aacaacttgc gcatggattt cggcttcgtt ccacaactta tcacgcagcg tgccgatgag
     3241 ttccgcgtct tcggcaaact gctccatcag aatcgcacgc gcaccgtcca acgcggcttt
     3301 ggcatcgggg acgttttcgt tcaggtagcc ttgcgcggca gcttccacgt ccagcggctg
     3361 ctcggcaagc aacacgtccg ccagcggctg caaaccgtgt tcgcgcgcga tttgcgcttt
     3421 ggtgcggcgt ttgggtttgt agggcagata caggtcttcc agcgcggttt tgttatcggc
     3481 ggcttcgatt tgcgcgcgga ggccgtctga aagcttgcct tgctcttcaa tgctttttaa
     3541 aacaacggct ttgcgctctt ccaactcgcg cagatattgc agccgctcgg caagccggcg
     3601 caactgcgta tcgtccaacc cgcccgtcgc ttccttgcgg tagcgggcga taaacggcac
     3661 ggtcgcgccg tcgtccaaaa gctcgacggc ggcggtgatt tgcgcggcag tcgcggagag
     3721 ttcttgggaa agaatttgag taatgttcat caatagaatt ccaacggaca ggccgtctga
     3781 aatttcagac ggcctgattt aaaaacaatc gctttaaggc agcgaattat aatattcgta
     3841 ggctttgtcc atatcttcaa actggtacat atgccctttt tccagcacca tcgcattatc
     3901 gcaatattgc ttcatggcgc tgtggctgtg cgacaccaag atgatggaac ggtctttgcg
     3961 cttttcaaac aactcgtact tacatttatc ggcaaaacgc gagtcaccaa ctgcaatcac
     4021 ttcgtcaatc aaatagcaat caaactctac cgccaacgac aaagcaaatg ccaaacgcgc
     4081 cttcatacct gaagaataac gcttgaccgg ctcgtaaaga tattgcccca actcggaaaa
     4141 ctcttcggta aagttcttca cataatcgat atcgacattg taaatccggc agatgaaacg
     4201 caaattgtcc ataccggtca gactgccttg aaacgcaccg gagaatgcca aaggccaaga
     4261 aatactcatt gtccgcttga tttcacccgt ggtcggcggc tcaacgccac tgatcaaacg
     4321 gatgagcgtc gatttacctg caccgttgcg gccgagaata ccgattttct cgcccttctc
     4381 cattttgaag ctaatatcgt gcaagactgt ccgccaacct tggcgggtca gatagcgttt
     4441 ggaaacgtgt tcaactgaaa tcattgcggc tcgactcctt tactgaattt actgaccatc
     4501 gccaagccaa acaacaacaa caccagattg cacaatacga tataccaagg attttcatag
     4561 gtaattacat cgctgccaaa atatccggca cggaacattt ctgtgccatg caccatcggg
     4621 atcatcaatg catactcttg cactttcgga ggcaaattat gtacaaaaaa gaatgcaccc
     4681 gataacggca tcatcacaaa agtcaatgtg ccccaaatct tgccaaacgg ctcgaaatta
     4741 aaggcaatcg aacaaatcac caaacccaaa ccaatcgcaa aaaaagccat caaaagccaa
     4801 gccatcagca tataaaacat atctgccggc atttcaatcc agccaatcgc aatcaatacc
     4861 gccataatca caatctgcgc aatggttgca ccagcaattt ccaaaatcat gcgcgccaag
     4921 atggtatcca aaactcttac attgcggtga taaagcaagc tggcatttga agaaatcgac
     4981 ccaactgccc gttttgaggc attacgccac atcatcaaca tcggatagcc agtaatcgca
     5041 aatgcgacaa tattcaaagt tgaatatcgg tctgccctta aaaatttcca catcaagacg
     5101 ataacgaatg tcatcagcaa cggctcaaca aacagccata aaaagccaat attattgcga
     5161 ccgtaacggg tgataatttc ccgcatcaaa agcgcaccga ttacgcgcct ttgaatggct
     5221 aaagattccc aaaatgatgt tttatgcaag gctttcatca gtttttatgc tcacgaatgc
     5281 tggcagtcaa caggctcaaa ataccataaa ccatcaagcc gataatcaga gtggcaacaa
     5341 tgttgtataa ccgtttaggc tcatgtgcca aatccggcag gctcggttgc gagatcactt
     5401 ccaaataaag ctgctgacgg tctgcttcaa ccttggcact ttccaaagaa gtcatggcgg
     5461 ctgccaactg ctgctctgcc aactggtttt ccaaatacac acgctgatac tcggcagcct
     5521 gattagacaa cgaagaatgc ccaccgcccg aaatggcacg taactgttgg tcaatttctt
     5581 tacgcaagct ctgctcacgc gcctgcaaac ccggaatctg cggattctcc ggagtgactg
     5641 ctttcacctg atccagctgg gtttgaatca caatcaattc atcttgcagc ttggaaacca
     5701 accccatttg cacttccgat tgcgctttca aatcaaaaac gccattggca atccggtaat
     5761 ccgtcagatt ctgagaggct tcctttaccc gctctgccgc cgttttcact acttcttccg
     5821 catagcgcac cgtatcagca cgtgcacgat cgttcaactg gttaatcaat gcttcacctt
     5881 gttttaacaa agcctcattg attttcttag attccagcgc atcaaaggaa gttacattca
     5941 acgtggaaat acccgaaacc gtatcaaaat tgatcatcac ctgattttta tagtattgat
     6001 aaaaagcctc ttcctcgcca cggaacccaa acccattaaa gcggctgaac gcatcacctt
     6061 tggtttcata aaactcacgc accggcaaga ttttacgcag ttcatccaaa gacgagcgcg
     6121 aacgcatata ctccccaacc gtgtaaatat catcttgcgc acgggcaaaa cctgtgccct
     6181 gcaaaatggc acccaggcca ttgagagaag attggctttt aggcgagcgc accacaaagc
     6241 tcgattgcga cgtaaaacga tcggaagcga agaagccgaa atacaccaac gaaattaccg
     6301 tagggataat caccgttacc caaaataaag ggcttagctt tttaatccaa cttttctttt
     6361 tcggcttttt acgctcggct ttggtttcag tggcaactgc cacaggaagt tgctcagaca
     6421 tcttaattac tcacattaat tagttaaatt attaatactg ttcgcgccac tggtaaccgg
     6481 cgagaacaca aacgacaaga atttctgcac ttcagccaac ggcgcattcg acacatacaa
     6541 tacatcttta ttcttcacag gaaagcgctg catagaaaat agcgaatgcg catcagccat
     6601 attcacacga tataccgttg gaatctctgc ctcactgcca taaccttgag caatccattt
     6661 atcctgacgt tctgccggca attccaccaa tggcgtatag cggaacacaa acacaccacg
     6721 cgcatcagaa cggcgatctt gcaaaccgcc catacggcca atggcttcag aaagcgataa
     6781 gcctctggct gaaaaaccga tttcttgtgt tctccccacc gcacccatag acgtaaaggt
     6841 atagggattg gtaatcatgg taaccacatc accgcgacgc agcaaaatat tttgtcgcgg
     6901 atttgcaact aaatcttcca aggcaacagt tcgtactaca ttgccacgtg tcagctgcac
     6961 attcgtatcc tgcacatttg ccgttgaacc acctaccgca gccaccgcat ccaacacacg
     7021 ctcaccggct gccgtcagcg gcatacgcac actattccca gcacgaatca ccgacacatt
     7081 cgccgcatta ttctgcacca aacgcaccat cacttgtggc tgattggcca tttttttcag
     7141 gcggccttta ataatttcct gaacctgacc aggcgtttta ccgaccaccg aaatatcgcc
     7201 aacaaacggc acagaaaccg taccacgtgc cgtgaccaac tgctctggca acttagtttg
     7261 atgcgcacta cccgagccca tcgaagaaag gccaccacca aacaatactg ccggcggcgc
     7321 ttcccaaatc ataatatcca atacatcacc aatatttagc gtaccagccg aagcataacc
     7381 atcgccaaac tgagtgaatg actgatttat ctgagcctta tataataact gagcaaccgt
     7441 atgattcaca tcaatcagct ccacttcagg aatttgaact tcagattgtt gccctaaaga
     7501 gacaattttt tttgcgctgg ggcctgatga aggaatcgca gagcatccta caattaaact
     7561 tccacacaat aataatactg cgtgacgaat ataaaatttc actttaaaca caagccaaat
     7621 cctaatataa ttataaatgg cctaattata gcacttaatc gaaataaatt tatgagtacg
     7681 tagagtataa ttagtattct tctttccaac ttccctatgc ttatagattc taaaatcatg
     7741 aaaagaattc tttgcattac aggtaccaga gccgacttcg gcaagctaaa acctttatta
     7801 gcctatattg aaaatcacct agaccttgaa ttgcatttga ttgtaactgg tatgcatatg
     7861 atgaaaacat atggcagaac ctgcaaggaa gtaactcgag aaaactatca acatacatat
     7921 ctgttttcaa atcaaatcca aggtgaacca atgggtgccg ttttaggcaa taccattacg
     7981 tttatctctc gtctatctga tgaaattgaa cctgatatgg tcatgattca cggcgaccgt
     8041 ttagaagcac tagcaggcgc aactgtaggt gcattaagca gccgtttagt ttgccatatc
     8101 gaaggtggtg aactatctgg tacagtagat gactccattc gtcattctat tagtaaactt
     8161 tctcatatcc acttggtagc aaatgaacaa gctgtcactc gcctagtgca aatgggagaa
     8221 aaaagaaagc atattcacat catcggctcc cccgatttag atgttatggc ctcttccacc
     8281 ctcccatcct tagaagaagt caaagaatat tacggtttac catacgaaaa ttatggtatt
     8341 tctatgtttc accccgtgac tacagaagca catttaatgc cacaatatgc ggcccaatat
     8401 ttcaaagcat tagaattaag tggccaaaat atcattagca tctaccctaa taatgacact
     8461 ggcactgaaa gtattctgca agaattatta aaatatcaat ctgataaatt tattgcattt
     8521 ccttccattc gatttgaata ttttttagtc ttattgaaac atgctaaatt tatggtcggc
     8581 aactcaagtg caggtattag agaagctcct ctctacggtg tcccttcaat tgatgttggt
     8641 acacgccaaa gtaaccgcca tatgggaaaa tctattattc atacagatta tgaaactaaa
     8701 aatatctttg atgcgattca acaagcatgc agtttaggca aatttgaagc agatgatacc
     8761 tttaatggcg gagatactcg caccagcaca gaaagatttg ctgaagtaat caacaatcct
     8821 gaaacgtgga atgtttctgc tcaaaaacgt tttatcgatt tgaatcttta aattatggaa
     8881 aaacaaaata ttgcggttat acttgcgcgc caaaactcca aaggattgcc attaaaaaat
     8941 ctccggaaaa tgaatggcat atcattactt ggtcatacaa ttaatgctgc tatatcatca
     9001 aagtgttttg accgcataat tgtttcgact gatggcgggt taattgcaga agaagctaaa
     9061 aatttcggtg tcgaagtcgt cctacgccct gcagagctgg cctccgatac agccagctct
     9121 atttcaggtg taatacatgc tttagaaaca attggcagta attccggcac agtaacccta
     9181 ttacaaccaa ccagtccatt acgcacaggg gctcatattc gtgaagcttt ttctctattt
     9241 gatgagaaaa taaaaggatc cgttgtctct gcatgcccaa tggagcatca tccactaaaa
     9301 accctgcttc aaatcaataa tggcgaatat gcccccatgc gccatctaag cgatttggag
     9361 cagcctcgcc aacaattacc tcaggcattt aggcctaatg gtgcaattta cattaatgat
     9421 actgcttcac taattgcaaa taattgtttt tttatcgccc caaccaaact ttatattatg
     9481 tctcatcaag actctatcga tattgatact gagcttgatt tacaacaggc agaaaacatt
     9541 cttaatcaca aggaaagcta aatgcaaaac aacaacgaat ttaaaattgg taatcgttca
     9601 gtaggttaca accacgaacc attgattatc tgtgaaatcg gcatcaatca tgaaggctct
     9661 ttaaaaacag cttttgaaat ggttgatgct gcctataatg caggcgctga agttgttaaa
     9721 catcaaacac acatcgttga agacgaaatg tctgatgagg ccaaacaagt cattccaggc
     9781 aatgcagatg tctctattta tgaaattatg gaacgttgcg ccctgaatga agaagatgag
     9841 attaaattaa aagaatacgt agagagtaag ggtatgattt ttatcagtac tcctttctct
     9901 cgtgcagctg ctttacgatt acaacgtatg gatattccag catataaaat cggctctggc
     9961 gaatgtaata actacccatt aattaaactg gtggcctctt ttggtaagcc tattattctc
    10021 tctaccggca tgaattctat tgaaagcatc aaaaagtcgg tagaaattat tcgagaagca
    10081 ggggtacctt atgctttgct tcactgtacc aacatctacc caacccctta cgaagatgtt
    10141 cgattgggtg gtatgaacga tttatctgaa gcctttccag acgcaatcat tggcctgtct
    10201 gaccatacct tagataacta tgcttgctta ggagcagtag ctttaggcgg ttcgatttta
    10261 gagcgtcact ttactgaccg catggatcgc ccaggtccgg atattgtatg ctctatgaat
    10321 ccggatactt ttaaagagct caagcaaggc gctcatgctt taaaattggc acgcggcggc
    10381 aaaaaagaca cgattatcgc gggagaaaag ccaactaaag atttcgcctt tgcatctgtc
    10441 gtagcagata aagacattaa aaaaggagaa ctgttgtccg gagataacct atgggttaaa
    10501 cgcccaggca atggagactt cagcgtcaac gaatatgaaa cattatttgg taaggtcgct
    10561 gcttgcaata ttcgcaaagg tgctcaaatc aaaaaaactg atattgaata atgcttatta
    10621 acttagttac tttattaaca gaggattggc tattacatat agctaattct cattaatttt
    10681 taagagatac aataatgcta aagaaaataa aaaaagctct ttttcagcct aaaaagtttt
    10741 ttcaagattc aatgtggttg acaacatctc cattttatct taccccccca cgtaacaatt
    10801 tatttgtcat atctaattta ggtcagctta accaagtcca aagcctaatt aaaatacaaa
    10861 aattaaccaa taatttacta gtaattttat atacttctaa aaacttaaaa atgcctaagt
    10921 tagttcatca atcagctaac aagaatctat ttgaatctat ttatctattt gagcttccta
    10981 gaagccctaa taatataact cctaaaaaat tactttatat ttatagaagt tacaaaaaaa
    11041 tccttaatat tatacagcct gctcatctct atatgctgtc ttttacaggc cactactcct
    11101 atctgattag tattgcaaaa aaaaagaata ttacgactca tttaattgat gaagggactg
    11161 gaacatatgc tcctttatta gaatcatttt catatcatcc aacaaaatta gaacgttatt
    11221 tgattggaaa taatcttaat attaaaggat atatagatca ttttgacata ttgcatgtcc
    11281 cctttcctga atatgctaaa aaaatattta atgcaaaaaa atataaccgg ttttttgcgc
    11341 atgctggagg aataagcatt aataataaca ttgcaaactt acagaaaaaa tatcaaatat
    11401 ctaaaaatga ctatattttt gttagtcaac gctaccccat ttcagatgat ctgtattata
    11461 agagtatagt agaaatctta aacagcataa gtttacaaat taaaggaaag atatttatta
    11521 aactacaccc aaaagagatg ggcaacaact atgtaatgtc tttatttcta aatatggtag
    11581 aaataaaccc tcggctggta gttattaatg aacctccttt tctaattgag tccctaatat
    11641 acttaacaaa tcctaaagga attataggcc tggcctctag ttctttaatt tatacaccat
    11701 tactctcacc ctcaacccaa tgtctttcta ttggagagtt aattattaac ttaattcaaa
    11761 aatattcaat ggtggaaaac actgaaatga tccaagaaca cttagagatt attaagaaat
    11821 ttaattttat taatatacta aatgatttaa atggggtaat aagtaaccct ctctttaaaa
    11881 cagaagaaac atttgaaaca cttcttaaat ctgcagaatt cgcatataaa tctaaaaact
    11941 actttcaggc tattttttac tggcaacttg ccagcaaaaa caatattacc ttattagggc
    12001 ataaagcatt atggtactac aatgcacttt ataatgtaaa acaaatttat aagatggaat
    12061 attcagatat tttttatatc gataatatct ccgtagactt tcatagtaaa gataaattga
    12121 catgggaaaa aattaaacat tattactatt ccgccgacaa tagaattggt agagatagat
    12181 aaaaatacta tgacttctga tctccatacg tatgcttgtt ttcaaaaagc agcattaaga
    12241 tagataagtg aagcttgatg agagccattt ttgtggtcat cgccaaggta cacgcggaga
    12301 gtaatcgaca acagagctgc tgtattctgg cttttaaagc ccagtagtaa agtttataag
    12361 gctgcatttc tcaatgtaaa acgcgatatt ttcacccatt attagggata agtagccagt
    12421 ggtttacact ggctactttc atagttatga agtactgggt ctgagtgaat tcaattattt
    12481 ccgtataaaa cacagtagac attttactaa tcggcaaaat tatattagaa gaattgagaa
    12541 ctactgaagt caggtaagat accattttgc acaaatttaa cggcatccct aaaaagcatt
    12601 ttgggtggta tttaaaggag tacaaaatgg ttttaactac agtatgctta gaaatctata
    12661 gccttacgcg ctttatatta aatcaccttt ctcaaccatt tagaatcata aaaaaacttt
    12721 ttaggatccc taattaactt tttaaatttt cttaaaaaaa tagttttagt tgattctata
    12781 ctattcaccg cagataaata ttcttggtaa tcatgttgca atggcacaac ctgctttaat
    12841 agcttattaa actcatcagt tgtaatcacc tttttagtag catagcaatc aagccaatcc
    12901 ggatctgcta tttttgggga agtgaaatct atttgatcaa aataaactaa aaccccacta
    12961 cctaaaaaat gctgatttgc ataggattta gaaaagccta agtctgcaac aacaccaaca
    13021 gggataccct tacattcagc ttcaaaaagc atagtagatg aataagataa acaatacccc
    13081 atttcttgga aggcttgcgc ggtacttttt cgctctattg tcaaattact cggcaactga
    13141 aactgctttg ccagctctat atacgaatgt ttatcctgat gcacagtaat atctttatct
    13201 gcaaccctta gcaaaatagt aaattctttc tcagggtatt tttcagccaa ggcaatcaat
    13261 ttttttaatg tataaattct ttcttctttt ttaaatggga ttttaacttg gtcaataaag
    13321 taaattttct ctccatgacg accttttgat gcgtggcgaa aaattggata accataaagt
    13381 atattttgac aacttaattt atattgtttc ttatattcct ctgcaattct aaaatcatgc
    13441 ttattattat ataaaacaat atcagccccc atacgagata gaatacttgc ctgatcaccg
    13501 aatactatac ctggaaacaa ggtaataatc aatggccttg aagcaatatt taattgcgca
    13561 ttctgcttaa agaacctttt taacaaccca tttccaaccg ataaaattac tgcgtcataa
    13621 tattgatgca tattcttaat aaagtaatca tcaatatgaa aaaaaattgc ttggtcttta
    13681 tctatccctg attctaataa ctgtcgattt gaaagaatgt tttctcgact tttgtggata
    13741 taaatatcaa tttgagcatc ttttatctct tttgcaacag catagcccga gttaaggaac
    13801 gagtcataac tggcaattag taatactttt ttcactattt tcccttaatt ctacaccata
    13861 tgaataaaat tatagtattt taccttagat atcaaatata cttaaccaaa aaaatctgaa
    13921 gaaatctact gctacttcag ggataattat ttcattaaga ttttacaacc attattatca
    13981 atctatcaaa gtatttttag tattttattc tatgaaaaaa attctcgtta ccggcggcac
    14041 cggttttatc ggctcgcata ccgttgtttc tttgctgaaa agcggccatc aagtcgtgat
    14101 tttggataac ctatgcaatt ccagcatcaa tatcctgcca cgcttgaaaa cgattaccgg
    14161 ccaagagatt ccgttttatc aaggcgatat ccgcgatcgt gagattttgc gccgtatttt
    14221 tgcggaaaac cgcattgatt cggtgattca ttttgccggc ttgaaagcgg tgggtgaaag
    14281 tgtggccgag ccgatgaaat attatgataa taatgtttcc ggcagcttgg tgttggcgga
    14341 agaaatggcg cgtgcgggcg tgtttagcat tgtgttcagt tcttcggcga cggtttatgg
    14401 cgatccgggc aaagtgcctt ataccgagga tatgccac
//
LOCUS       DAXHNE010000037        13835 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.037, whole genome shotgun sequence.
ACCESSION   DAXHNE010000037 DAXHNE010000000
VERSION     DAXHNE010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13835
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.037"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(171..1496)
                     /locus_tag="ACFM4Z_000856"
     CDS             complement(171..1496)
                     /locus_tag="ACFM4Z_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFC6020919.1"
                     /translation="MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPDLAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGKYMADKGYLKLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFF
                     RELTGSGNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            1707..2546
                     /gene="efeU"
                     /locus_tag="ACFM4Z_000857"
     CDS             1707..2546
                     /gene="efeU"
                     /locus_tag="ACFM4Z_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFC6020920.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGLGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKRQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIIVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVITWFLCGSRPSETLTRKE
                     ELK"
     gene            2543..3709
                     /gene="efeO"
                     /locus_tag="ACFM4Z_000858"
     CDS             2543..3709
                     /gene="efeO"
                     /locus_tag="ACFM4Z_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFC6020921.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGA
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRIHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            3973..5238
                     /gene="efeB"
                     /locus_tag="ACFM4Z_000859"
     CDS             3973..5238
                     /gene="efeB"
                     /locus_tag="ACFM4Z_000859"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFC6020922.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYNYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(5308..5637)
                     /locus_tag="ACFM4Z_000860"
     CDS             complement(5308..5637)
                     /locus_tag="ACFM4Z_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFC6020923.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVR
                     DANGAVLQNGDTVILIKDLKVKGSSMTIKQGTKVKGIRLQEGDHNISCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(5712..7082)
                     /gene="glmU"
                     /locus_tag="ACFM4Z_000861"
     CDS             complement(5712..7082)
                     /gene="glmU"
                     /locus_tag="ACFM4Z_000861"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFC6020924.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHCIGGKPMVERVIDTAA
                     ALNPQNICVVIGHGKEQVLNTVKRDVVWVEQTEQLGTGHAVKTALPHLSAEGRTLVLY
                     GDVPLIDVETLKTLLEAAGNEVGLLTDVPADPTGLGRIIRDSNGSVTAIVEEKDADTA
                     QKAVKETNTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVRVRA
                     SHLAAGVNNKRQLAELERIFQTGQAQELLKAGVTLHDPARFDLRGRLKHGQDVVIDAN
                     CIFEGEIELGDNVEIGANCVIKNAKIGANSKIAPFSHLESCEVGENNRIGPYARLRPQ
                     AKLAADVHIGNFVEVKNAAIGNGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVSPVKIGNKVTTGAGSTITRNVEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            complement(7137..7409)
                     /locus_tag="ACFM4Z_000862"
     CDS             complement(7137..7409)
                     /locus_tag="ACFM4Z_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HFC6020925.1"
                     /translation="MRKTFLFLIAAAALLSGCAWETYQDGNGKTAVRQKYPVGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(7435..8115)
                     /locus_tag="ACFM4Z_000863"
     CDS             complement(7435..8115)
                     /locus_tag="ACFM4Z_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFC6020926.1"
                     /translation="MQENPTVWLFDLDNTLHDADAGIFTLINRAITRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRKSHPIDAILTRLHGMPETQNTLSR
                     LKGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKSHALPFIDTCARDMAQLARYAETLSER
                     RQNHYNTP"
     gene            8174..9175
                     /locus_tag="ACFM4Z_000864"
     CDS             8174..9175
                     /locus_tag="ACFM4Z_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6020927.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPGSAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPELAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            9269..10117
                     /locus_tag="ACFM4Z_000865"
     CDS             9269..10117
                     /locus_tag="ACFM4Z_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFC6020928.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            10144..10629
                     /locus_tag="ACFM4Z_000866"
     CDS             10144..10629
                     /locus_tag="ACFM4Z_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HFC6020929.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(10731..12299)
                     /gene="msrAB"
                     /locus_tag="ACFM4Z_000867"
     CDS             complement(10731..12299)
                     /gene="msrAB"
                     /locus_tag="ACFM4Z_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HFC6020930.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            12445..13710
                     /gene="ftsY"
                     /locus_tag="ACFM4Z_000868"
     CDS             12445..13710
                     /gene="ftsY"
                     /locus_tag="ACFM4Z_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFC6020931.1"
                     /translation="MFSFFRRKKKQETPAPEEAQVQETAAKAESEVTQIVENIKEEVE
                     SLTESVKGQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAVAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         2948 a         3536 c         4153 g         3198 t
ORIGIN      
        1 gttccgtact atttgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcca
       61 ctataattcc gtcaaataag aaaggaattt tgtgtctgcg gtatcgcaaa acttcgcctt
      121 aatgcgcccg attgcctagg gatgggcttc agatggcatt gttttccggt ttacgggcgg
      181 tattcgggct tcataccgtt gggcagaagc tgccagacat atcccgtggt tttctgtttg
      241 ccggcaagtt cgccggcttc gtcgccgtat ccccaaaaat aatccacgcg caccgcgcct
      301 ttaattgcgc tgccggtatc ctgcgccata atcaggcggt tgagggcttt gcgggtaacc
      361 ggatgggcgg tggcgacaaa taagggcgca cccaaggtaa tgtagtgccg gtcgactgcg
      421 ccggcatatt cccccatcag cggcgtgccc agtgcgccga cagggccgtc attgccgctt
      481 ccggtaagct cgcggaaaaa gatatagctg gggttttgac ccaaaacttc ggcgaggcgt
      541 tgcggatttt gccgcatata agacttaatg ccctgcatgg aggtttgtcc gagtttgagg
      601 tagcccttat ccgccatgta ttttccgatg gaaacatacg gatgctcgtt tttgtcggca
      661 tagccgatgc ggatgtattt gccggacggg gttttcagac ggcccgagcc ttggatgtgc
      721 ataaaaaaaa gttcgacagg gtcttcggcg taaccgagta tcggggcttt gccgtcaagc
      781 gcgccgccgt tgatttggtt gcgcgtgtgg taggggagga agcggcttcc ttcaaacctg
      841 cctttgattg ccgttgtgcg cgcggtgatg gggaatcggg agaggtcggc ggtatgtgtg
      901 ccgccggtat tgtcgattgt gccgctgttt ttccccgtct gcctgatgcg gacaagggct
      961 tttccgctcc gcaaaccggc aggcaggggg acggagataa aatcgtcggg aataccgtaa
     1021 atcgggaagc gggcttgtgc cgtccgcctg tcgtcgccct tcagcaccgg ttcgtaatag
     1081 ccggtaaccg taccggcaag gcttccgttg cctgcaacct gccacggcgt gaaatagcgt
     1141 tcaaaaaact gttttgcctg aaaggaatgg acgggggttt gaaaggcttg ggcgcacaca
     1201 tcctgccagc cttggcggtt tttcaaattg gcgcagccga ggcggaagga ttgcaggctt
     1261 ttggcgaaat cctgcgccgc ccagtggggc agggacaggt gcggtacaac ggtatagacg
     1321 gccccgccgc cgccgaccgt cgttccggcg aggtcgggga tgccgaccgg ccggtccggg
     1381 ccgttgatga cggatgtgtc gggttgcgga aaggtttgga tgctcttgct ttggcaggcg
     1441 gcgaggatgg cggcggcgat gccgtacagg gcggcgcgga ataggtattt tttcataatg
     1501 gacaatgttg ccggcagtaa taagaaagat ggtttcgggc ggcgttgcgg cagccgtgga
     1561 gaggggattt taacacaggg cgcagctgca gcctgcggaa ctttccgccg cgcggtactg
     1621 cagataaaaa taacttgcat ttgtatttac aagcaatgaa aatattccga taatatatta
     1681 ttcatcatcc ttgttcgttc gcgtttatgc tggtcgcttt tttaattatg ttgcgcgagg
     1741 gtatcgaagc cgcgctcatc gtcggcatcg ttgccggttt tctgaaacag tccggacatt
     1801 ccaaactgat gcctaaggtc tggttcgggg ttgtccttgc ttctttgatg tgtttggggc
     1861 tggggtacgg catccattcg gcaacaggcg agattcccca aaagcagcag gagttcgtcg
     1921 tcggcatcat cggtttggtt gccgttgcca tgctgactta tatgatttta tggatgaaaa
     1981 aggcggcgcg ttcgatgaag cggcagcttc aggattctgt gcaggcggct ttgaaccgtg
     2041 gcagcggcca aggatgggcc ttggtcggta tggcgtttct tgccgtggcg cgcgaaggtc
     2101 tagagagcgt ttttttcctg cttgccgtat tcaaacagag cccgacgtgg cagatgccgg
     2161 ccggtgcggt agcgggggtt ttggctgccg ccgtgattgg cgcgttgatt tatcagggtg
     2221 ggatgcgcct gaatctggcg aagtttttcc gttggacagg ggcgtttctg attatcgttg
     2281 ccgctggtct gcttgccggt tcgctgcgtg cgctgcacga agcaggcatt tggaacgcgc
     2341 ttcaggacat tgtgttcgac tcatcaaaat atttgcacga agacagcccg ttgggcgtgc
     2401 tgctcggcgg atttttcggc tataccgacc atccgacgca gggcgaggcc ttggtttggc
     2461 tgctgtacct tattcccgtc ataacttggt ttttgtgcgg cagcaggccg tctgaaactt
     2521 taacccgtaa agaggagctg aaatgagaaa attcaatttg accgcattgt ccgtgatgct
     2581 tgccttaggt ttgaccgcgt gccagccgcc ggaggcggag aaagctgcgc cggcagcgtc
     2641 cggtgaggcg caaaccgcca acgagggcgg tgcggtcagt atcgccgtca acgacaatgc
     2701 ctgcgaaccg atggaactga ccgtgccgag cggacaggtt gtgttcaata ttaaaaacaa
     2761 cagcggccgc aagctcgaat gggaaatcct gaagggcgtg atggtggtgg acgagcgcga
     2821 aaacatcgcc cccggacttt ccgataaaat gaccgtcacc ctgttgccgg gcgaatacga
     2881 aatgacctgc ggccttttga ccaatccgcg cggcaagctg gtggtaaccg acagcggctt
     2941 taaagacacc gccaacgaag cggatttgga aaaactgtcc caaccgctcg ccgactataa
     3001 agcctacgtt caaggcgagg ttaaagagct ggtggcgaaa accaaaacct ttaccgaagc
     3061 cgtcaaagca ggcgacattg aaaaggcgaa atccctgttt gccgacaccc gcatccatta
     3121 cgaacgcatc gaaccgattg ccgagctttt cagcgaactc gaccccgtca tcgatgcgcg
     3181 tgaagacgac ttcaaagacg gcgcgaaaga tgccggattt accggcttcc atcgtatcga
     3241 atacgccctt tgggtggaaa aagacgtgtc cggcgtgaag gaaattgcag cgaaactgat
     3301 gaccgatgtc gaagccctgc aaaaagaaat cgacgcattg gcgtttcctc cgggcaaggt
     3361 ggtcggcggc gcgtccgaac tgattgaaga agtggcgggc agtaaaatca gcggcgaaga
     3421 agaccggtac agccacaccg atttgagcga tttccaagcc aatgtggacg gttcgaaaaa
     3481 aatcgtcgat ttgttccgtc cgctgatcga ggccaaaaac aaagccttgt tggaaaaaac
     3541 cgataccaac ttcaaacagg tcaacgaaat tctggcgaaa taccggacta aagacggttt
     3601 tgaaacctac gacaagctgg gcgaagccga ccgcaaagcg ttacaggcct ctattaacgc
     3661 gcttgccgaa gaccttgccc aacttcgcgg catactcggc ttgaaataag ccgcaagcgt
     3721 tcagacggta tttggcggca gataccgtct gaagttttat agtggattaa caaaaaccag
     3781 tacggcattg cctcgccttg ccttgccgta ctatttatac tgtctgcggc ttcgtcgcct
     3841 tgtcctgatt tttgttaatc cactatatcc gccatatatt gcagggcggg atttcaacct
     3901 gccgctatcg gttaatggaa aaacggcgtg cagggatacc catcctgctg cacggatatt
     3961 gaaggaaaca ccatgagcaa aaaccaaccc gcacaaccga ccaggcgcac tctttttaaa
     4021 accgcgatcg cagccggcgc agtcggcgca atcggaggtt atctcggcgg caaaaaacag
     4081 ggcgaaaccg ccgaacgcac cgccgaaagc caacactcgc cccaagccta tccctgctac
     4141 ggcgaacatc aggcaggcat cgttacgccg cagcaggcgt tttcgattat gtgcgccttc
     4201 gacgtaaccg cgcaaagtgc caagcagctg gaaaacctgt tccgcacgct gaccgcccgc
     4261 atcgagtttc tcacccaagg cggcgaatac caagacggcg acgacaaact tccgccagcc
     4321 ggcagcggca ttttgggcaa agccttcaac cccgacgggt tgaccgttac cgtgggggtg
     4381 ggcagcagcc tgtttgacgg ccggttcgga ctcaaagaca aaaaaccgat tcatttgcag
     4441 gaaatgcgcg acttctccaa cgataagctg caaaaaagct ggtgcgacgg cgatttgagc
     4501 ctgcaaatct gtgccttcac ccccgaaacc tgccaagccg ccctgcgcga catcatcaaa
     4561 cacaccgtcc aaaccgccgt tatccgctgg agtatcgacg ggtggcagcc taaatccgaa
     4621 cccggcgcga tggcggcgcg caacctgttg ggcttccgcg acggcacggg taaccccaaa
     4681 gtttccgacc ccaaaactgc cgacgaggtt ttgtggacgg gggtggccgc caacagcctc
     4741 gacgaaccgg agtgggcgaa aaacggcagc tatcaggcag tccgccttat ccgccacttt
     4801 gttgagtttt gggacaggac gccgcttcaa gagcaaaccg acattttcgg gcggcgcaaa
     4861 tacagcggcg cgccgatgga cggcaaaaaa gaagccgacc aaccggattt tgccaaagac
     4921 cccgagggga ataccacgcc caaagacagc catatacgcc tggcgaatcc gcgcgatccc
     4981 gagttcctta aaaaacaccg cctcttccgc cgcgcctaca actattcgcg cggactcgcc
     5041 tcaagcggac agcttgatgt cgggctggtg ttcgtctgtt atcaggcaaa ccttgcagac
     5101 ggcttcatct tcgtgcaaaa cctcctcaac ggcgaaccgc tggaagaata catcagcccc
     5161 ttcggcggcg gctatttctt cgtgctgccc ggcgtggaaa aaggcggctt cttgggacaa
     5221 gggctgctgg gcgtataaac ctgcctgacg gacaaatgcc gtctgaatcc gataaggggt
     5281 tcagacggca ttttagtgtt tgggcggtca ggcttttttg acgaattcag attttaaatt
     5341 catcgcgctg ccgtcgattt tgcaggagat gttgtgatcg ccttcctgta ggcgtatgcc
     5401 tttgactttt gtaccttgtt tgatcgtcat cgagctgcct tttaccttga ggtctttgat
     5461 gaggatgacg gtgtcgccgt tttgcagcac tgcgccgttg gcatcgcgca cttgagccgc
     5521 aaggtcggcg gcggattcgg tttcattcca ttcgtgggcg cattcggggc agatgtattg
     5581 tccgccgtct tcataagtgt attcggaggc gcattgtggg catgggggta atgacatggt
     5641 ttgcagtcct tatcggatgt ttgttttggg gtaccgtctg aaacctgaaa ccggcttcag
     5701 acggcatagc tttattgttt gtctttttca ggacgcaccc agccttcgat gacggtttgg
     5761 cgggcgcggg cgagggcgag tttgttgtct tcgacattgc gggtaatcgt gctgcccgcg
     5821 cctgtggtta ctttgttgcc gattttgact ggggagacca agacgcagtt tgaaccgatg
     5881 cggacttcgt cgccgatgac ggttttgtgt ttgtgcacgc cgtcgtagtt ggcaataatc
     5941 gtgcctgcgc cgaagttggt tttgctgccg acttcggcat caccaatgta ggtgaggtgg
     6001 ttggctttgg tgccgttgcc gatggcggcg tttttgactt cgacgaagtt gccgatgtgt
     6061 acgtcggcgg ctagtttggc ttgcggacgc aggcgggcgt atgggccgat tcggttgttt
     6121 tcgccgactt cgcagctttc gaggtgggag aagggggcga ttttgctgtt tgcgccgatt
     6181 ttggcgtttt tgatgacgca gtttgcgccg atttcgacgt tgtcgccgag ctcgatttcg
     6241 ccttcaaaga tacagtttgc atcaatcacg acatcttgtc cgtgtttcag acgaccccgc
     6301 aaatcgaaac gtgccgggtc gtgcagggta acgcctgctt tgagcaattc ttgcgcctgt
     6361 ccggtttgga agatgcgttc gagttcggcg agctggcgtt tgttgttcac gccggcggcg
     6421 aggtgggagg cgcgcactcg gacgggatga actttaatac cgtcggcaac ggctttggcg
     6481 atgaggtcgg ttaaataata ttcgccttgg gcattgttgc tggaaaggct gttcagccag
     6541 ttttccagtt tggcgttggg caggacgagg atgccggtgt tggtttcttt cacggctttt
     6601 tgggcggtat cggcgtcttt ttcttcgacg atggcggtta cgctgccgtt gctgtcgcgg
     6661 atgatgcgcc ccaagcctgt cgggtcggcg ggcacgtcgg tcaacagccc gacttcgttg
     6721 cctgcggctt cgagcagggt tttgagggtt tcaacgtcaa tcaggggaac gtcgccgtac
     6781 agtaccagcg tgcggccttc ggcggaaagg tggggcaggg cggttttgac ggcgtggccg
     6841 gtgccgagct gttcggtttg ttcaacccaa acgacatcgc gtttgacagt attcaaaact
     6901 tgctctttgc cgtgtccgat gacgacgcag atgttttgcg gattcagtgc ggcggcggtg
     6961 tcgataacgc gctcaaccat gggcttgccg ccgatgcagt gcagcacttt gggcattttg
     7021 gaatacatgc gcgtgccttt gccggcggcg aggatgacga tgtttaaagt gttttgcggc
     7081 atagcgtttt cctgtgcaat gccgtctgaa gcggcttcag acggcatagg gtgggtttat
     7141 cggttttgaa atttgggctt ttgccagtgt tggcgatgct cttcgtcggc gttgttgccg
     7201 gtttggttgg gtaacacggc atggcgttcg ggacggtatt ggttgtagtt catatttttc
     7261 gagtagctgc cgtcttggta ataaacgggc gtgccgacgg gatatttttg acggacggcg
     7321 gttttgccgt tgccgtcttg ataagtttcc cacgcgcagc ccgacaaaag ggcggcggca
     7381 gcgatcagga agaggaaggt tttacgcatg gcttttcttt cgtattttcg ggggttaggg
     7441 ggtattgtaa tgattttggc ggcgttctga caaagtttct gcataccgag ccagttgcgc
     7501 catatcgcgt gcgcaggtgt cgataaaggg cagcgcgtgg gattttgcac cgaaccggac
     7561 ggttttcatg cccaatgctt ttgcctgatg caggttgtcc gcgctgtcgt ccaccataat
     7621 gcagcattcg ggcggtacgt ccaaaaggcg gcagacattg agatacgctt gcggattggg
     7681 tttgtacagc agtccgaaat catccgtgcc gaacagtgcg tcgaaacggt tttccaaacc
     7741 gagtgcgccg acaacggcac ggacgtaaaa cgacgggccg ttggaaaaaa ccgccttgcg
     7801 cccttttagg cggctcaggg tgttttgtgt ttcaggcatg ccgtgcagcc tggtcaggat
     7861 tgcatcgatc ggatggcttt tgcgcaaaaa ttcggcgatg tcgatttcgg gatggtggat
     7921 ttgcagtccg gcgagcgttg cgccgtagcg gtgccaatag tcttgacgca ggtcggacgc
     7981 ggcagattcg gagagtttga ggcggcgtgc catatagcgt gttatagcgc ggttgatgag
     8041 tgtgaagatg ccggcatcgg catcgtgcag cgtgttgtcg aggtcgaaca gccacacggt
     8101 cgggttttct tgcatgttga accgtgaaaa tttgttagaa tgttatttta cagcgaatag
     8161 aggaggactc ggaatgaaac ggaaaatttg gctgctgccg ctgctggcgg tttcggcata
     8221 cctgcaggcg cagacggaag tcaggctggc ggtgcataag tcgttcagcc tgcccaaagg
     8281 gctgattgcg cgcttcgagc gggcaaacga tgcgaaggtg tcgattattc aggcgggcgg
     8341 cgcgaacgaa atgctcaaca aactgatttt gagccgcgcc aacccgatcg ccgacgcggt
     8401 gtacggcttg gacaacgcca atatcggcaa ggcgcgggaa atgggcattt tggcggcggc
     8461 gcaacccgga tccgcccccg tcgcggtcgg gctgccttcg gctttggcgg tcgattacgg
     8521 ctatgtgtcc atcaattacg acaaaaaatg gtttgaaggc aaaaagcttc ctctgccgca
     8581 aaccctgcag gatttgaccc gccccgaata taaaaaccta ttggtcgtgc cgtctcccgc
     8641 cacgtcgtcc ccggggctgg gcttcctgat ggcgaacatc agcggtctgg gcgaagaagg
     8701 cgcgttcaaa tggtgggcac agatgcggca gaacggcgtg aaggtcgcca aaggctggag
     8761 cgaggcgtat tacaccgact tttcgcacaa cggcggcgcg tatccgctgg tggtcggtta
     8821 tgccgccagc ccggcggcgg aagtgtattt ttccaaaggc aaatacagcg agccgccgac
     8881 gggcaacctg tttttaaaag gcggcgtatt ccgccaggtc gaaggcgcgg cggtcttgaa
     8941 gggcgcgaaa cagccggaat tggcggcaaa actggtgcaa tggctgcaaa gtcgggaagt
     9001 gcagcaggcg gttccgtccg aaatgtgggt ttaccccgcc gtcaaaaaca cgcgcctgcc
     9061 cgacgtgttc cgcttcgccc aagccccgac gcacaccacc gcccccgcgc agcgcgatat
     9121 tgatgcgaac cagcgcggat gggtttcccg ttggattaga acggttttga aataaaacaa
     9181 acataccgcc ccgcagggct tcatacggca tttttacacc tgtgccgatt acgccgcacg
     9241 gggcggatgt tcgatcaaga ggaaaacaat ggacttcaaa caatttgatt ttttacacct
     9301 gatcagtgtt tccggttggg agcatctggc tgaaaaggcg tgggcgttcg ggctgaacct
     9361 tgccgccgcg ctgcttattt ttttggtcgg aaaatgggcg gcgaaacgca ttgtcgctgt
     9421 gatgagggcg gcgatgacgc gcgcgcaggt cgatgccacg ctgattagtt ttttgtgtaa
     9481 tgttgccaat atcggcttat tgattttggt gattattgcc gcattgggca gattgggcgt
     9541 ttccacaaca tccgtaaccg ccttaatcgg cggcgcgggt ttggcggtgg cgttgtcctt
     9601 gaaagaccag ctgtccaatt ttgccgccgg tgcgctgatt atcctgttcc gcccgttcaa
     9661 agtcggcgat tttatccgcg tcggcggttt tgaaggatat gtccgagaga ttaaaatggt
     9721 gcagacttct ttgcggacga ccgacaacga agaagtcgtg ctgcccaaca gcgtggtgat
     9781 gggcaacagc atcgtcaacc gttccaccct gccgctttgc cgcgcccaag tgatagtcgg
     9841 cgtcgattac aactgcgatt tgaaagtggc gaaagaggcg gtgttgaaag ccgccgtcga
     9901 acaccccttg agcgttcaaa acgaagagcg gcaggccgcc gcctacatca ccgccttggg
     9961 cgacaatgcc atcgaaatca cattatgggc ttgggcaaac gaagcagacc gctggacgct
    10021 gcaatgcgat ttgaacgaac aagtggtcga aaacctccgc aaagtcaata tcaacatccc
    10081 gttcccgcaa cgcgacatac acatcatcaa ttcttaaacg ccgtctgaaa ggggagtggg
    10141 aaaatggacg cgctgcacac catcgcccga aacctgacga aaaaacgtca aaccgtaagc
    10201 tgtgccgagt cctgtacggg cggaatgctt gccgccgcat tcacaagcgt tgcaggcagt
    10261 tcgcaatggt tcgaccagag ttttgtaaca tacagcaaca aagccaaaga agaccgcttg
    10321 ggcgtgttgc ccgaaaccct gctcgaacac ggcgcggtca gccgccaaac cgtctatgag
    10381 atggcgcgcg gcgcgaaagc cgtggcgcag gcggattacg ccgtcggtat ttccggcatc
    10441 gccggaccgg gcggcggcag cgaaagcaaa cccgtcggca cggtttggtt cgggtttgcc
    10501 tttccgggcg gaagttgcga agcaatgcgc cgttttgacg gcaaccgcga atccgtccgc
    10561 gcgcaggcgg tcgccttcgc gttggaacgg ttggcggggc tgattgaaaa cggcggcgat
    10621 gctgtctaaa caaaatctcc gtctggacag aatccccgcc ggataaaaaa taccgtctga
    10681 aacgttgcag gtttcagacg gtattttgtc gggttaggcg gcggtgcggc ttatttcact
    10741 ttactcttca acgcgccgta gcctgccgcg tccatttgtt ccagcgggat gaatttcaag
    10801 ctcgcgccgt tgatgcagta gcgcagtccg cctttgtcgc gcgggccgtc ggggaagacg
    10861 tgtcccaaat gcgaatcggc ggcgtggctg cgcacttcgg tgcggcgcat gttgtagctg
    10921 aaatcatcgt gttcggtaac ggattttgca tcaatcgggc gcgtgaagct cggccagccg
    10981 cagccggaat catatttgtc ggcggagctg aacaaaggtt cgccgctgac aacgtccaca
    11041 taaatgccgg gtttgaacaa atggtcgtat tcgtggctga aggcatattc ggtcgcgctg
    11101 ttttgggtaa cttggtattg ctcttcggtc agggtgcgtt tgagttcggc gtcactcggt
    11161 tttttatacg ttgccgcgtc gaagcctttg ccttgcgggg cggtcttggt tttgcccggc
    11221 agcggttcgt cggctttgcg gatatcgatg tggcagtagc cgttggggtt tttaatcagg
    11281 tagtcctgat ggtattcctc ggcatcgtag aagtttttca gcggctcgtt ttcaacaacg
    11341 aggggcagtt ggtatttttg ctgctcgcgt ttgagggcgg cggcgatgac ggctttttcg
    11401 gcggggtcgg tgtagtacac gccgctgcgg tattgcgtac cggtgtcgtt gccctgtttg
    11461 ttgaggctgg tcggatcaac gacgcggaag aaatattgca ggatgtcgtc taggctgagt
    11521 ttgtcggcat cgtaggtcac tttaacggtt tcggcgtggc ccgtatggcg gtaggacacg
    11581 tcttcatagc tcggattttt cgtgttgccg ttggcgtagc cggataccgc gtcaaccacg
    11641 ccgtcgatgc gttggaaata ggcttccaag ccccagaagc agccgccggc gaggtaaatg
    11701 gtgcgcgtgt tcatgatttt tgaatccttt ttctgagtgt cgggtttgta gaacgaatgt
    11761 ttcaaactgc ccaaatcggc attcgggtcg cggattaacg ccaacgcctg cgcttcgttg
    11821 atgctgcctt tgacgatgcg ctgcacgtcg ccgtctttac cgattaacgc ccacgaaggg
    11881 taaacgctga tattcaggct ttgggcgatc gtgccgccgt tgtcggttac gacgggcagc
    11941 ttgggataat tcaaaccggc ataccatttt tggaagtcgc cgtctttttt ctcgtgcaaa
    12001 aagcccggag acgcgacggt aatcaggttg gcggagctga attttgcatc ttgcgcccat
    12061 ttttcggtct gtcccaattc ggacagacac aaaggacacc agcttgccca aaatttaatc
    12121 agcgtcggtt tgtctttttt caagtaaaca ctggcggggc ggttgtctgc ggttttcaaa
    12181 gtggataaag tgtgcggcac ggtcgcggct ccggcatcga cgattttggg cgaacaagca
    12241 cccagcgcaa gcaggcagcc gaacttggcg caaagggaaa agaaagtacg gtgtttcatt
    12301 ttaatgtttc ctgtgtggac ggtttgcatg attagacgtt tgagatgccg aaaccttaca
    12361 gcccggattt tcagacaacc ttgccgcgca aaatacgcta caatacaccc tatttcaagt
    12421 ttctaaaatt aaaaggaaaa ttcaatgttc agcttcttcc gtcgcaagaa aaaacaggaa
    12481 acgccggctc ccgaggaggc tcaagttcag gaaactgcag caaaagcaga atccgaagtt
    12541 actcaaatag ttgaaaatat taaagaagaa gtcgaatctt taacggaaag cgtcaaaggg
    12601 caggtcgaat cggctgttga aaccgtcagc ggtgcggttg aacaggtaaa ggaaaccgtt
    12661 gccgagatgc tgtctgaagc agaggaagcg gcggaaaaag cagcggaaca agtcgaagcg
    12721 gcaaaagaag ccgttgccga aaccgtcggc gaggctgtcg ggcaagttca agaagccgtt
    12781 gcgacaactg aagaacacaa gctcggttgg gcggcgcgtt tgaaacaagg tttgaccaaa
    12841 tcgcgcgaca aaatggcgaa atcgctggcg ggcgtgttcg gcggcggaca aatcgacgaa
    12901 gatttgtacg aagagctgga aaccgtgctg attaccagcg atatgggcat ggaagccacc
    12961 gaatacctga tgaaagacgt gcgcgaccgc gtcagcctca aagggctgaa agacggcaac
    13021 gaattgcgcg gcgcgctgaa agaagccttg tacgacctga ttaagcctct agagaaaccg
    13081 ctggttttgc ccgaaaccaa agagccgttt gtcatcatgc ttgctggcat caacggcgcg
    13141 ggcaaaacca catccatcgg caaactcgcc aaatatttcc aagcgcaggg caaatctgtg
    13201 ctgcttgccg ccggcgatac tttccgcgcc gccgcgcgtg agcagcttca agcttggggc
    13261 gaacgcaaca acgtaaccgt gatttcgcaa accacgggcg attccgccgc cgtgtgcttc
    13321 gatgccgtcc aagccgccaa agcgcgcggc atcgacattg tgctggccga caccgccggc
    13381 cgcctgccca cgcagcttca cttgatggaa gaaatcaaaa aagtgaaacg cgtgctgcaa
    13441 aaagccatgc ccgacgcgcc acacgaaatc atcgtcgtgc ttgatgccaa tatcggtcaa
    13501 aacgccgtca accaagtcaa agcctttgac gacgcattag ggctgactgg tttaatcgtg
    13561 accaaactcg acggcacggc aaaaggcggc atcctcgccg cgcttgcctc cgaccgcccc
    13621 gttcccgtcc gctatatcgg cgtgggcgaa ggcatagacg acctgcgccc gtttgacgcg
    13681 cgcgcgtttg tggacgcact gctggattga gccgaaatgc cgtccgaaaa cagcagaccg
    13741 atgccgtcat tcccgcgcag gcgggaatcc agaccttggg ataacggcaa tattcaaagg
    13801 ttatctgaaa gtccgagatt ctggattccc acttt
//
LOCUS       DAXHNE010000038        13570 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.038, whole genome shotgun sequence.
ACCESSION   DAXHNE010000038 DAXHNE010000000
VERSION     DAXHNE010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.038"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(261..596)
                     /locus_tag="ACFM4Z_000869"
     CDS             complement(261..596)
                     /locus_tag="ACFM4Z_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFC6020932.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQGL
                     PPGVGDFFR"
     gene            complement(676..2790)
                     /gene="dnaX"
                     /locus_tag="ACFM4Z_000870"
     CDS             complement(676..2790)
                     /gene="dnaX"
                     /locus_tag="ACFM4Z_000870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFC6020933.1"
                     /translation="MAYQVLARKWRPKTFADLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCESCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYEPAALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAQEMAACAV
                     GFDNALGELAILLQHLALIQAVPNALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLSLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELKSPSAQTAEKETAAK
                     KPQPRPEAETAQTPVQTASAAAMPSEGKTAEPVTNQENNDIPPWEDAPDETAAGTAQA
                     SAKSIQTASEAGTPPKNQVSKNEAADNETDAPLSEVPSENPIQATPNNEALETEAFAH
                     EAPAKPFNGYSFPNDDYLVEDGAEIPPPDWEHAAPADAEEENNADESSNNEDHTPYAP
                     PPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARATADKKR
                     LDKIRDTLAQAYGLQLTLQTQDWRDEAGRETPAMQDKRVQAEDRQKAQALLEADPAAQ
                     KILQAFGAQWQPESLELAANRP"
     misc_feature    complement(1562..1601)
                     /gene="dnaX"
                     /locus_tag="ACFM4Z_000870"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            complement(2885..4861)
                     /gene="mutL"
                     /locus_tag="ACFM4Z_000871"
     CDS             complement(2885..4861)
                     /gene="mutL"
                     /locus_tag="ACFM4Z_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFC6020934.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELAGGGIRLIRVSDNGSGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSEATEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPEQSLHERIAAIVGDDFQT
                     ASLEIDSGSGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHTLNKALADTRADLTE
                     SVSNAGEVLHDITGVTPAPMPSENDSENLFDRASNYPTGNKPDTRNAFGASGKTAPMP
                     YQSAYAPQQRSLSLRESRAAMNTYAELYKKTDDIDLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSPSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGKLQSQRLLIPVTFAASHEECAALADYAETLAGFGLELSDMGGNTLAVRAVPAML
                     GKADVVSLAKDVLNELAQVGSSQTIEEHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            5004..5189
                     /locus_tag="ACFM4Z_000872"
     CDS             5004..5189
                     /locus_tag="ACFM4Z_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HFC6020935.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            5200..5868
                     /locus_tag="ACFM4Z_000873"
     CDS             5200..5868
                     /locus_tag="ACFM4Z_000873"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6020936.1"
                     /translation="MTTHLSNVAPDLQNYLNSIGEPEHPVLTRLREKTEHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPRYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            5868..6584
                     /locus_tag="ACFM4Z_000874"
     CDS             5868..6584
                     /locus_tag="ACFM4Z_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HFC6020937.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRPEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGTLVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAMFKIGECQYRLQQKDIARATWRSL
                     IQAYPSSPAAKRAAAAVRKR"
     gene            6632..7117
                     /gene="purE"
                     /locus_tag="ACFM4Z_000875"
     CDS             6632..7117
                     /gene="purE"
                     /locus_tag="ACFM4Z_000875"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFC6020938.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANETPELAQKLADFRAKQEQAVLSMELEQ
                     I"
     gene            complement(7300..8754)
                     /locus_tag="ACFM4Z_000876"
     CDS             complement(7300..8754)
                     /locus_tag="ACFM4Z_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFC6020939.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPTEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(8751..9452)
                     /locus_tag="ACFM4Z_000877"
     CDS             complement(8751..9452)
                     /locus_tag="ACFM4Z_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFC6020940.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEVERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            complement(9449..10228)
                     /locus_tag="ACFM4Z_000878"
     CDS             complement(9449..10228)
                     /locus_tag="ACFM4Z_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFC6020941.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKA"
     gene            10513..11919
                     /locus_tag="ACFM4Z_000879"
     CDS             10513..11919
                     /locus_tag="ACFM4Z_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6020942.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADL
                     NESPLDMQLAVISYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQIAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSPLWIWVFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVAGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGTNLTRNTPS"
     gene            12031..13557
                     /locus_tag="ACFM4Z_000880"
     CDS             12031..13557
                     /locus_tag="ACFM4Z_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFC6020943.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQAG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPAPRKTYPNEPEAKLWKRIAAKILSLLPIESLL
                     "
BASE COUNT         2721 a         3659 c         3834 g         3356 t
ORIGIN      
        1 tttcagacgg catttaagcg gtacggatgt gtaaataatg gtagggtggg cttcagcctg
       61 ccgattcccg ctattcttgc cgtttttgcg ttcttatcat tctcactgtt tttaccgttc
      121 acgccgaatc ttgcaaactg atgccgtcat tcccgcgaag gcgggaatcc agacccctcg
      181 gcacggaaat ttatcgagta aaacggtttc ttagattcta cgttctagat tcccgcctgc
      241 gtgggaatga cggtcgggga tcagcggaag aagtcgccca ctccgggggg tagaccttgc
      301 gtgaatgcgc ccattgtttt gtttgcggtt tcttcggctt tgcctcgggc ggatttgagg
      361 gcggcgagga tgaggtcttc aagcatttct ttgtcgtcgg cggcttcttg aatcaaatcg
      421 gggctgatgt cgattttgcg tacttcgtgc gcgcaggtca ttgtgatttt gaccaggccg
      481 ttgcctgctt cgccttcgat ttcggtttcg gcgagtttgg cttgcgcttt tttcatattt
      541 tcctgcattt gctgcgcctg tttcatcagg ccgcctaatc cggcttttcc gaacatactg
      601 aatactcctg tctgttttga ttgaataagg gaaacggcaa tgccgtccga agggttcggg
      661 cggcattata tctgtttatg gccggtttgc cgccaattcc agtgattcgg gctgccattg
      721 cgcgccgaat gcttggagga ttttttgtgc ggcggggtcg gcttcgagca atgcttgtgc
      781 tttttgcctg tcttcggctt ggacgcgctt gtcctgcatc gcgggggttt cccggccggc
      841 ttcgtcacgc cagtcttggg tttgcagggt gagttgcagc ccgtaggctt gggcaagggt
      901 gtcgcggatt ttgtcgaggc gttttttgtc ggcggtggcg cgtgcttcgg cggtcattgc
      961 caaaaccatc agaccggtgt cgggatggta ttccgtccac gcggagtgtt gcgccggcat
     1021 ttgcgccgcg ccgagtttgc gggcgaagtg ccggacgatg gctgcccagt tttcggtgga
     1081 aaattcgggc ggcggggcgt atggcgtgtg gtcttcgttg ttgctgcttt cgtcggcgtt
     1141 gttttcttct tccgcatcgg caggggcggc gtgttcccaa tcgggcggtg ggatttctgc
     1201 gccgtcttct acgaggtagt catcattcgg aaagctgtaa ccgttgaaag gttttgcagg
     1261 agcttcgtgt gcaaatgctt ctgtttcaag ggcttcatta ttcggtgttg cctgaatggg
     1321 gttttcagac ggcacttcgg acaagggggc atcggtttcg ttgtcggctg cttcgttctt
     1381 ggaaacttgg tttttgggcg gcgttccggc ttcggatgcc gtctgaatgc tttttgccga
     1441 tgcttgcgcc gtgccggctg cggtttcgtc cggcgcgtct tcccaaggcg gaatatcgtt
     1501 gttttcttga ttggtaacgg gttcggcagt tttgccttca gacggcattg ctgctgcgga
     1561 tgccgtctga acgggtgttt gggcggtttc cgcttcaggg cgcggttggg gcttttttgc
     1621 ggcggtttcc ttttcggcgg tttgtgccga tggggatttt agttcggtat tttcaatcac
     1681 ggcatttgca tcacacgatg ctgccgccaa gggcgcaaac gccagcatac gcagcagggt
     1741 catcataaag ccggcgtatt cgtcgggggc gaggctgagg tcgcgtttgc cgtggacggc
     1801 gatttggtag taaagctgga tttgttcgcc gcttatggtt tgggcgaggc ggtgcaaaat
     1861 atcggaatcg gggtcgtcgt gcgccaaggc attcggcact gcctgtatca gggcgaggtg
     1921 ttgcagcagt atggcaagtt cgcccaaggc gttgtcaaag ccgacggcac acgccgccat
     1981 ttcctgcgct ttggcggtca gggctgcgcc gtcttggttg atgatgcctg tcagcagttc
     2041 gtaaaggtat tgtttgtcaa ccgcgccgat catttggcgg acatcgtttt cggcaacttt
     2101 gcccgaacct agggcgatgg cttggtcgag caggctcaag gcatcgcgca tcgatccggc
     2161 ggcggcacgt cccaaaagtt gcagggcggc gggttcgtag gcgatttttt cgctgtcgag
     2221 gacgtgggcg aggtggtcgg caacctgttg cgcggtcata ttgcgtaaga cgaattgcag
     2281 gcagcggctc aagacggtaa cgggaacttt gtgcggatcg gtggtggcga ggatgaattt
     2341 gacgtgttcg ggcggctctt ccagcgtttt gagcatagcg ttgaacgcgc ttttggaaag
     2401 catatgcact tcgtcgatga tatagacttt gtattttccg gcggtcggtg catattgggc
     2461 gttttccaag acttcgcgga tgttgtcgat gcctgtgttg gaggcggcgt cgatttccag
     2521 caggtcgacg tagcgtccgg catcgatctg cgtacagctt tcacatacgc cgcaaggttc
     2581 gccgtgttgc gcgttttcgc agttgaggct tttggcaagg atgcgggcga tggtggtttt
     2641 acctacgccg cgcgtgccgg tcagcaggta ggcgtggtgc agcctgcctt cgtccagggc
     2701 gttttgcagg gctttgacga cgtgttcctg accgactaag tcggcaaagg ttttgggccg
     2761 ccattttcgg gcgagaactt gataggccat gtttttctct tggtttcggt cgtgatgttt
     2821 ctgtcggtgc gtcggaatgc cgtctgaacg gcggtctcgg gcggcgtatt ctagcacttt
     2881 cggcttactg tccgcgcaag aacagtgcgt ccaattcttt caaagtcagt ttgacccaag
     2941 tcggcctgcc gtggttgcac tggttgctgc gcggcgtatt ttccatatcg cgcagaaggg
     3001 cgttcatttc gggcagggtg agccggcggc cggcgcggat cgagccgtgg caggacatgg
     3061 tggcgaggat gcggttttcg tgttcctcga tggtttggct gctgccgact tgggcgagtt
     3121 cgtttaatac gtctttggcg agcgagacga catcggcttt gccgagcatg gcgggaactg
     3181 cacggacggc gagggtgttg ccgcccatat cggataattc cagcccgaag cctgccagcg
     3241 tttcggcata atcggcaagg gcggcgcatt cttcgtggga cgcggcaaag gttacgggaa
     3301 taagcaggcg ttggctttgc agcttgccgt tttcctgccg ttggcgtttc attttctcgt
     3361 agttgacgcg ttcggcagcg gcgtgcatat cgatgagcag caggctgtct tcggcttggg
     3421 caaggatgta gatgcctagc aattgggcaa tggcgaaacc gagcggcggc agttcggatg
     3481 gggacgggat gccgtctgaa agcggcgtat ctgtttgggg agcaggcgtt tcagacggca
     3541 tattgccgaa acgtgcctgc tcgaatcggc ttaactcaag gtcgatgtcg tcggtttttt
     3601 tgtagagttc ggcataggtg ttcattgccg cgcggctttc gcgcagggac aggctgcgtt
     3661 gttgcggcgc atatgcggac tgatagggca taggcgcggt tttgcctgat gcaccaaagg
     3721 cattgcgcgt atcgggtttg ttgcctgtcg ggtagttgga tgcgcgatca aacagatttt
     3781 cgctgtcgtt ttcagacggc attggggcag gcgtaacgcc ggtaatgtca tgcaacactt
     3841 cgcctgcgtt gctgacgctt tcggtcaagt cggcgcgtgt gtcggcaagg gctttgttga
     3901 gcgtgtggaa cacgagttgg tgcacctgct ggctgtcgcg gaagcggatt tcggttttgg
     3961 tcgggtggac gttgacatcc acggcttcgg gcggcaggtc gaggaaaagg acgaaagcgg
     4021 gggtgagggc gttgtgcaaa acgtcgcggt atgcctgctt gacggcgtgg agcatcactt
     4081 tgtcgcggac aaaacgatgg ttgacgaagc agtattgttt gtcggttttg cctttggcga
     4141 aagtcggctt ggcaatcgcg ccatagagcc gcaatgcgcc gctgccgctg tcgatttcca
     4201 aagatgccgt ctgaaaatca tcgccgacaa tggcggcaat ccgttcgtgc aggctttgtt
     4261 cagggagttt gaacacttgt ttgccgtcgc gtttgagcga gaaggcaatg tgcggatgcg
     4321 ccagcgcgag gcgttcgagc atagtggcgc agtgggcgta ttcggtggct tcagacttga
     4381 ggaacttgcg ccgcgcgggg gtgttgaaga agagttcggc ggcttcgatg gtggtgccga
     4441 cggggtgggc ggcggcggtg gggctgctga gtttgccgtc ttcggctttg acttgggttg
     4501 cgtgcgaact gtcttcttga cggctggtca gggtcaggcg gctgacggag gcgatgcttg
     4561 ccaagccttc gccgcgaaag cccatgctgg caacgtgttc taaatcgttt aaggttttga
     4621 ttttgctggt agcgtgtcgg tggagcgcaa gctcgatgtc gtccggatga ataccgctgc
     4681 cgttgtcgct gacgcgaatc aggcggatgc cgccgcccgc cagctcgaca tcaatcgccg
     4741 ttgcgcctgc atcgatactg ttttcaacga tttctttcaa ggcgttggca gggcgttcga
     4801 ccacttcgcc ggcggcgatt tggttgacaa gatggtcggg cagggcggca atgcgtggca
     4861 taaggtaggc ttccgttgca aaaacgcttt attctataat aaaacccctt acttttctgc
     4921 ctgtattctt gataaaagcc gtttatcccg tttggaaaat accagtataa tcacgccgta
     4981 tcccgtaaaa ttggagcgca aagatgtatc actaccaatc cgaagccaca caattcctca
     5041 accgcctgat tgaagaaaaa cctgagttgg aacagcagcg tttggaaaac cgggggcttt
     5101 tgtgggatgt cgaactcaat cccgaagaac aggaaaactt cgaggcggca aaagtagcga
     5161 aaaaacctta tacctattac caagactgaa cccgccgaaa tgacgaccca tctgtccaat
     5221 gtcgcaccgg acctgcaaaa ctatctgaac agcatcggcg aacccgaaca tcccgttttg
     5281 acgcggctgc gtgagaagac cgagcatcac cgtatgggca aaatggcgat tgcgcgcgaa
     5341 caggcggcgg ttttggtttg gctggcaaag ctgattcgcg cggaaaaata tctggaagtc
     5401 ggcgtgttta ccggatacag cagcaccgcg cttgcattgg cgttgccaga acacgggcgg
     5461 attaccgcct gcgacatcaa tgtaaccttt accgatacgg cgcgtcaggt ttggaacgag
     5521 gcaggtgtgg cacataaaat cagcctgcac ctgcaacccg cattgctgac attggatgat
     5581 ttgattgcac agggtgaagc cggaagctac gatttggcac tgatagacgc agacaaaccg
     5641 cccacgccgc gatatttcga gcgttgcctc aagcttgtcc gtcaaggcgg catcatcgcc
     5701 atcgacaata ttttgctgaa cggaagggtg atgcgcgaag cggcttccga tgcgccgccc
     5761 agcgtcggca tcctcaaaga tttcaatcaa aacctgccga acgatacgcg gattgtcccc
     5821 atcaccctgc ccgtcggcga cggcttgacc ctgcttctga aaaaataatg aagaccaaat
     5881 taccgctttt tatcatttgg ctgtccgtat ctgccgcctg ttcttcccct gtttcccgca
     5941 atattcagga tatgcggccc gaaccgcagg cagaggcagg tagttcggac gctattccct
     6001 atcccgttcc cactctgcaa gaccgtttgg attatctgga aggcacactc gtccgcctgt
     6061 cgaacgaagt ggaaacctta aacggcaaag tcaaagcact ggagcatgcg aaaacacacc
     6121 cttccagcag ggcatacgtc caaaaactcg acgaccgcaa gttgaaagag cattacctca
     6181 ataccgaagg cggcagcgca tccgcacata ccgtcgaaac cgcacaaaac ctctacaatc
     6241 aggcactcaa acactataaa agcggcaggt tttctgccgc tgcctccctg ttgaaaggcg
     6301 cggacggagg cgacggcggc agcatcgcgc aacgcagtat gtatctgttg ctgcaaagca
     6361 gggcgcgtat gggcaactgc gaatccgtca tcgaaatcgg agggcgttac gccaaccgtt
     6421 tcaaagacag cccaaccgcg cccgaagcca tgttcaaaat cggcgaatgc caatacaggt
     6481 tgcagcagaa agacattgca agggcgactt ggcgcagcct gatacaggct tacccgagca
     6541 gcccggcggc aaaacgcgcc gccgcagccg tgcgcaaacg atagtttttc catttttccg
     6601 ctttttaccc catccgcacc aaggaggatt tatgatacaa atcggcatca tcatgggcag
     6661 caacagcgat tggcccgtta tgcggcaggc agcgcagttt cttgaagagt tcggcgtaga
     6721 atatgaggcg cgcgttgttt ccgcacaccg caccccggat ttgatgttcc aatacgccga
     6781 aaccgcacgg gcgcgcggca tcaaagccat catcgcgggt gcgggcggcg cggcgcattt
     6841 acccggtatg gttgccgcca agaccaccgt ccccgttttg ggcgtacccg tccccagcaa
     6901 atacctgcgc ggcgaagatt cgcttttatc gattgtacaa atgcccaaag gcgtacccgt
     6961 cgccacattc gccatcggcg aggcaggcgc ggcaaatgcc gcattgttcg ccgtttccat
     7021 gctcgccaac gaaacccccg aactggcgca aaaactggca gacttccgag ccaaacagga
     7081 acaggctgtt ttgagtatgg aattggaaca gatttaaaac ccgccgtccg cgaatatata
     7141 gtggcttaaa tttaaaccag tacggcgttc cctcgccttg ccgtactatt tgtactgtct
     7201 gcggcttcgt cgccttgtcc tgatttttgt taatccacta tacaaatgcc gtctgaagcc
     7261 ctgtttcggg tttcagacgg catttttgcg aacaactttt tatgcctgtt ctttttggcg
     7321 cattttttct gccattagtt cgtgcagggt tttcttcgcc ggtttcatcg gcacgcggtt
     7381 ttgcgtccaa cccaactgtt tgcgcggggt caggttgcgg aacttggtgg ctgcccaacc
     7441 gaaggcgcgg taggttttgc tgccgctgaa aataccgttg aatgtgcgcc acgccatttg
     7501 ttcgccgaag gtatgcgatg cgccttgccc tcggatgggg tgcggcacgg tttcggtcgg
     7561 cgaacgttgc gcttcaacgc gcaaacgctg catttgttcg gtaatcggga tgcgtaccgg
     7621 acagacttcc acacacgcac cgcacatggt acaggcggtc ggcaggtcgc gggtggcatc
     7681 cagacccaac aggtgagggg aaataatctc gccaatcggg ccgggatagg ttgtgccgta
     7741 tgccgcgccg ccgatgcggg tataaaccgg gcaatggttc atacacgcgc cgcaacggat
     7801 acattgcagg gtgcggcgca tttggtcttc ggcataagcc tggctgcggc cgttgtcgag
     7861 cagaaccaag tgcatttctt gcggaccgtc taattcttca ctgcggcgcg ggccggtaat
     7921 catattgaaa taggttgtga tgttctgacc gatggcggag cgcggcagca gactgtacaa
     7981 gggtgggatg tcggacaatt tcgccaccac tttttcaatg ccggtaatag cgatatgcac
     8041 gggcggaacg gtggtactca agcgaccgtt gccctcgttt tccaccagac acagcgtacc
     8101 tgtttcagca acggcaaagt ttacgccact caagccgaca tcggcagtgc tgtaaatatc
     8161 gcgcagggct ttgcgggcga agccggtcag ttggtccaca tcgtctgtaa gcggtgtgcc
     8221 gaggttttgg tggaacagtt cgctcacctg ttctttggtt ttatggattg caggcatcac
     8281 gatatgggtc ggtttttcgc ctgccatttg gacgatgaac tcgcccaagt cgctttctac
     8341 cgctttaatg ccttttgctt caagataatg gttcagctcg atttcctcgc tgaccatcga
     8401 tttgcctttg accatcagct tgccgttttt ggctgtgatg atgtcgtgga taatttggca
     8461 ggcttcggtc ggggtttctg cccagtgcac tttcacgccc aacttagtca ggttttcttc
     8521 cagctgctcc agcagggctg gcaatttaga caatgagcgc tgacggacgt gttcgcacaa
     8581 atcgcgcagg ctttgcagct cttcttcgtc ggtcaaaacg gctttgcgtt tggtcatcag
     8641 catatccatc gcggtacgca ggcttttgcg caaaggcttg tcttgaaggg aaattgcggc
     8701 gttttgcttg aaagtttccg gcttcatgtg aaatttgatg gtttgcgtag tcatgcgttt
     8761 tcctccaaat cggcagggga aatgtggtcg ggcaggatgg cgaggatgac caaatcgcgc
     8821 gggccgtgcg cgccgtaagc aagcgtcagt tggatgtctg cggttttgga cgggccggaa
     8881 atcaggaata cattggtcgg cataccgttt tccaccagtt tttcgccttc gacggcatta
     8941 tgaaactcgt tgtacatctt ggacgtatcg aacaggcaga aatgcacggg aggaacgagg
     9001 cttaaagtac gcggctcttc ggggctggaa aacagcatca gcgtgccggt gcgggcgatg
     9061 ccgcattgcg cgccgctgaa gcccgcatcg atgttcgtga aaaactcggt tttccaagta
     9121 tcgatttcgc gctcgaaggc aatcggttcg atattgctgt ccgccaatgc ggcacgggca
     9181 atttgtccgt gttcggtcgc caagggcagc aggatgtttt tcaaaccctt gccttctgcc
     9241 gcttcgcgga aaacctgcat ccaattgctt ttcgtcaccc aataaatttc ggttttgacc
     9301 gcacgcatag cggcagccca atgtttcaga cgctcaactt cgctgcccca agaaacaccc
     9361 atttcacggt aataatcaaa aaccgcaggt tcttccatcg gcaatgcgtc ggcttttttc
     9421 agttttgcca aaatattttc acgcgcgctc atgctttgcc tccggtgcgt tccaacaaga
     9481 aggatgcgat atgtttcgga cgcggcatat ccggctcgtc cttggcgatt ttgccgccga
     9541 tgttcatcat acagccgcag tccgcgctga tgatttcggt tgcgccggtt tctttcagcg
     9601 cggcgacttt gtctgttacc attgcgccgg aaatatcggc ttgtttgacg gagaatgtgc
     9661 cgccgaagcc gcaacattcg ctttcgtggt cgtggacgat gcgttcgacg ttttccatac
     9721 cgtcaatcag ttgccagcct gaaagatgga cattcatttc gcggcgggcg gcgcaggaag
     9781 tgtgaacggc gactttgagc ggttcgccct tgtcttcggg tttgaaaccg atggcaagca
     9841 ggaaatgggt aaactcgatg atgcggccgg cgcaatccac agccctttcc tcgtactcgc
     9901 tgcctttaaa cagcgtcggc cagtggtgtt tcatcatgcc gccgcacgag ccggacggca
     9961 cgacgatcgg ccagttttca gggaaaaggt cgagttgtgc tttggcgaca tcgaaggctt
    10021 cggtcggatg gccggatgaa taggcaggct ggccgcagca gctttgcgcc atcgggaaat
    10081 ggacgcgtat gccctgctgc tcgattaggg taatggcatc catgcctgct tcgggcataa
    10141 aaagatcaag gacgcaagtg ccgaaaaaat agacatcggt cggattgctg tcgtatcgga
    10201 tgatttttgg gggaatggtt gcgctcatgt tttttgataa cgggaaaccc gtttttctct
    10261 gtagaaaggt aagcgtttac tttaagtaat tgactgttgc gggtcaagtc taattttaaa
    10321 aaataatccg gtttttctta caaactgccc ccataacgct tactgtacct taatctgatg
    10381 gttttcgata ataattatca ttacaatgca atgccggttc gtttgcttgt gaacattcaa
    10441 gatgccgact ctgacggcat tcagacagca tctgaaaaca ataacggcac aaggatgggg
    10501 tacaatcgcc ctatggaaaa aaacacccta tccgcccgcg caccatcccc ttggctgccg
    10561 cttctgctgg caattgccat ttttatgcag atgttggatg cgaccatttt aaataccgca
    10621 ctgcctgaaa ttgccgccga cctgaatgag tcgcctctgg atatgcaact ggcagttatt
    10681 tcctacacgc tgacggttgc cctgctgatt cctttgagcg gttatttggc ggacaggttc
    10741 ggaacgaaaa aagtcttttt cggttcgatt gccgttttta tgctcggatc ggcattgtgc
    10801 gccgcatcgg gttcgctgtt tgaattgacg ctttcccgtg tcgttcaggg catcggcggt
    10861 tcgatgctgg ttccgatacc gcgtctgacc atcttgcgtg tgtacgacaa gtccaagctg
    10921 ctcaatgcca tcaattatgc ggttatgccc gcattaatcg ggccggtttt agggcctttg
    10981 gcgggcggtt atttggtcga atacgcttcg tggcactgga ttttcctgct caacctgccc
    11041 atcggtctgc tgggtttcat attgggacgc aacatcatgc ccgatattaa aggcagtaat
    11101 atctctttag acttcaaagg ttatctgatt ttttctgccg ccgcgtgcct cttgttactt
    11161 tcggcagaaa gcctgtcgca cgcgctgcct ccgtattttg cactgttgcc gctgtgcggc
    11221 ggactgctgt ttgcacgccg ttatttccga catatgaaaa ccgcgtccaa accgatttat
    11281 tccgccgacc tgtttctgat acgcactttc cgtctgggac tggcgggcaa tctgttcagc
    11341 cgtctcggca tcagctcgat tccttttctg atgcccctga tgtttcaaat cgctttcggc
    11401 ttcggcgcaa gcctgtcggg ttggctggtc gcacccgtcg ccctgtcttc gctgctggtc
    11461 aaaccgctga ttgcaccgct catgaaacgt ttcggctacc gcacggtact gctttggaac
    11521 accaagctgc ttgccgcctt catcatgctg ctcgccctgc ctgacggaaa ctcgccgctg
    11581 tggatttggg ttttcctctc gctggcgatc ggcgcgtgca actccctaca gttttctgcc
    11641 atgaacacac tgaccctcgc cgatttgcgc ccgcaacaaa caggcagcgg caacagcctg
    11701 atggcggtca accaacagct tgccatcagc atgggcattg ttgccggcgc attaatcctt
    11761 aaaaactgga catttctgat accggcttct tcaggtctgc attccgcctt ccgtatgacc
    11821 ctgctcagca tcggcggcat cacccttgca tcatcgctgg ttttcaaacg gctgcacgtt
    11881 tcagacggca ccaacctgac acggaacaca ccgtcctgaa gcggtccaca cgcaaaactt
    11941 ttacccgttt caacgtttgg attatgatac cgcacttcca tgcgcgccaa ccccaaaaca
    12001 caggcaatgc cgtctgaaac catatccctg atgaaaacac gcagcctaat ttccctttta
    12061 tgcctccttc tctgttcatg ttcttcatgg ttgcccccac tggaagaacg gacggaaagc
    12121 cgtcatttca atacttccaa acccgtccgc ctggacaaca tcctgcaaat ccggcacacc
    12181 cctcatacca acgggctatc cgatatctat ctgttgaacg acccccacga agcctttgcc
    12241 gcccgcgccg cccttatcga atctgccgaa cacagcctcg atttgcaata ctacatctgg
    12301 cgcaacgaca tttccggcag gctgctgttc aacctcgtgt accttgccgc agaacgcggt
    12361 gtgcgcgtac gcctgctgtt ggacgacaac aacacgcgcg gattggacga cctcctgctt
    12421 gccctcgaca gccatcccaa tatcgaagtg cgcctgttca accccttcgt cttacgaaaa
    12481 tggcgcgcac tcggctacct gaccgacttc ccccgcctca accgccgcat gcacaacaaa
    12541 tcctttaccg ccgacaaccg cgccaccata ctcggcggac gcaatatcgg cgacgaatac
    12601 ttcaaagtcg gtgaggacac cgttttcgcc gacctagaca tcctcgccac cggcagcgtc
    12661 gtcggcgaag tatcgcacga cttcgaccgc tactgggcaa gccattccgc ccacaacgcc
    12721 acgcgcatca tccgcagcgg caacatcggc aagggtcttc aagcactcgg atacaacgac
    12781 gaaacgtcca gacacgcgct cctgcgctac cgcgaaaccg tcgaacagtc gcccctctac
    12841 caaaaaatac aggcaggacg catcgactgg cagagcgtcc aaacccgcct catcagcgac
    12901 gaccctgcaa aaggactcga ccgcgaccgc cgcaaaccgc cgattgccgg gcggctgcaa
    12961 gacgcgctca aacagcccga aaaaagcgtc tatctggttt caccctattt cgtccccaca
    13021 aaatccggca cagacgcact ggcaaaactg gtgcaggacg gcatagacgt taccgtcctg
    13081 accaactcgc tacaggcgac cgacgttgcc gccgtccatt ccggctatgt caaataccga
    13141 aaaccgctgc tcaaagccgg catcaaactc tacgagctgc aacccaacca tgccgtccct
    13201 gccacaaaag acaaaggcct gaccggcagc tctgtaacca gcctgcacgc caaaaccttc
    13261 attgtggacg gcaaacgcat cttcatcggt tcgttcaacc tcgacccccg ttccgcgcgt
    13321 ctcaacaccg aaatgggcgt tgttatcgaa agccccaaaa tcgcagaaca gatggagcgc
    13381 acccttgccg ataccacccc cgaatacgcc taccgcgtta ccctcgacag gcacaaccgc
    13441 ctgcaatggc acgatcccgc cccccgaaaa acctacccga acgaacccga agccaaactt
    13501 tggaaacgca tcgccgcaaa aatcctatcc ctgctgccca tagaaagttt attatagaaa
    13561 tatagcggat
//
LOCUS       DAXHNE010000039        13451 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.039, whole genome shotgun sequence.
ACCESSION   DAXHNE010000039 DAXHNE010000000
VERSION     DAXHNE010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13451
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.039"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(78..278)
                     /locus_tag="ACFM4Z_000881"
     CDS             complement(78..278)
                     /locus_tag="ACFM4Z_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020944.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRALREVAEISARVLLG
                     AAAATLFWYLFMLYFKHIPDSY"
     gene            421..1560
                     /locus_tag="ACFM4Z_000882"
     CDS             421..1560
                     /locus_tag="ACFM4Z_000882"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HFC6020945.1"
                     /translation="MSQNASVDIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALADYLGIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSRNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            1557..2138
                     /gene="metW"
                     /locus_tag="ACFM4Z_000883"
     CDS             1557..2138
                     /gene="metW"
                     /locus_tag="ACFM4Z_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HFC6020946.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRILERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            2255..3100
                     /locus_tag="ACFM4Z_000884"
     CDS             2255..3100
                     /locus_tag="ACFM4Z_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NMB0938 family lipoprotein"
                     /protein_id="HFC6020947.1"
                     /translation="MKNKTSSLLLWLAAMMLTACSPSKEDKTKENGASAASSTASAAS
                     SSAPQTDLQPAASAPDNVKQAESVPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(3150..3710)
                     /gene="efp"
                     /locus_tag="ACFM4Z_000885"
     CDS             complement(3150..3710)
                     /gene="efp"
                     /locus_tag="ACFM4Z_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFC6020948.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKIEIDTRTGEFRKRA"
     gene            complement(3753..4901)
                     /gene="earP"
                     /locus_tag="ACFM4Z_000886"
     CDS             complement(3753..4901)
                     /gene="earP"
                     /locus_tag="ACFM4Z_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HFC6020949.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDVVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDSGALRKRLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPLTLLLAGAQIIDS
                     LKQNGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETASAHRCLSDDLNGGGALSAT
                     QRLECWQILQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(4920..5198)
                     /locus_tag="ACFM4Z_000887"
     CDS             complement(4920..5198)
                     /locus_tag="ACFM4Z_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020950.1"
                     /translation="MSRNLLVRWLVVCLIPLATLAVFVANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIVLLVVYLFHYFGAF"
     gene            5378..6319
                     /gene="miaA"
                     /locus_tag="ACFM4Z_000888"
     CDS             5378..6319
                     /gene="miaA"
                     /locus_tag="ACFM4Z_000888"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFC6020951.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSASERAFVPHHLIDIIPPTESYSAARFVEDCTRLVGEISSRGRFALIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIERALEVYYLTGKPMSEHLGRQSPHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWKYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSCTRLIEAAKRFFGA"
     gene            6323..7042
                     /gene="tadA"
                     /locus_tag="ACFM4Z_000889"
     CDS             6323..7042
                     /gene="tadA"
                     /locus_tag="ACFM4Z_000889"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFC6020952.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTLPQEITQHDKTRLFAELKNHPPVAAFPPQEEMEHFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIASAHNTCIADCNVSCHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLNRFFQNKRKG"
     gene            7047..7535
                     /locus_tag="ACFM4Z_000890"
     CDS             7047..7535
                     /locus_tag="ACFM4Z_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HFC6020953.1"
                     /translation="MTALLVILALALIAAGTAGIVYPALPGLALMFAGTWLLAYSGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALVGSIIGIFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLIVGTTFKIGCAVSILFILLVKYIAY
                     LF"
     gene            7601..8353
                     /gene="rlmB"
                     /locus_tag="ACFM4Z_000891"
     CDS             7601..8353
                     /gene="rlmB"
                     /locus_tag="ACFM4Z_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFC6020954.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYVQEGKNDVRTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVASGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGNADLYHCDLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
     gene            complement(8415..9806)
                     /locus_tag="ACFM4Z_000892"
     CDS             complement(8415..9806)
                     /locus_tag="ACFM4Z_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFC6020955.1"
                     /translation="MSGHMGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAMILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            complement(9821..10075)
                     /locus_tag="ACFM4Z_000893"
                     /pseudo
     CDS             complement(9821..10075)
                     /locus_tag="ACFM4Z_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10113..10988
                     /gene="dapA"
                     /locus_tag="ACFM4Z_000894"
     CDS             10113..10988
                     /gene="dapA"
                     /locus_tag="ACFM4Z_000894"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFC6020956.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQVDIALARELNNRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEN
                     GQAKVRAALKASGQL"
     gene            10997..12193
                     /gene="bamC"
                     /locus_tag="ACFM4Z_000895"
     CDS             10997..12193
                     /gene="bamC"
                     /locus_tag="ACFM4Z_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFC6020957.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDKVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTVLALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(12250..13182)
                     /gene="pip"
                     /locus_tag="ACFM4Z_000896"
     CDS             complement(12250..13182)
                     /gene="pip"
                     /locus_tag="ACFM4Z_000896"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HFC6020958.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIRKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEDVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIRHIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLVDALVQAVEDILPHLL"
BASE COUNT         3438 a         4042 c         3294 g         2677 t
ORIGIN      
        1 cacctcctac cgcaagccct tttcatcaaa tatgccgtct gaaacagaaa aatgcgtttc
       61 aggcggcatt ttttccgtca atacgaatcc ggaatgtgtt tgaaatacag cataaacaga
      121 taccaaaaca gcgtcgcggc ggccgcaccg agcagcaccc gggcactgat ttcggcaact
      181 tcgcgcaggg cgcgtttctg cctgcggctg aagaggcggt tgataatcag cgcggcaagt
      241 gcgaaaaaga aaaaaatacg gaaaagtctg cctatcatat cgggaagcga aaagtatggt
      301 tcgggcgcgg cagcatggga aaggatattt tagcgcatcg ggcttcttgt ccgaccgtgt
      361 caaaaagtca tgccgtttgc gatataataa cgcttttcgc cgcatttgtt ggaacaggat
      421 atgagtcaaa atgcctcggt ggacattgta acgccccaaa aaattccgtt tgagatgccg
      481 ctggttttgg aaaacggtaa aactttgccg cgtttcgatc tgatgattga aacctacggc
      541 gagctgaatg ccgaaaaaaa caatgcggtt ttaatctgcc acgcgctgtc gggcaaccat
      601 catgttgcgg gcaggcattc cgcagaggat aaatatacgg gctggtggga caatatggtc
      661 ggacccggca aaccgattga tacggaacgt tttttcgtgg tcggtttgaa taatctgggc
      721 ggctgcgacg gcagcagcgg gcctttgtcg attaatcctg aaacgggtag agaatacggc
      781 gcggattttc cggtggttac ggtgaaggac tgggtaaaat cacaagccgc gcttgccgat
      841 tatctcggca tcgggcaatg ggcggcggtt gtcggcggca gcttgggcgg catgcaggct
      901 ttgcagtggg cgatttccta tcccgaacgc gtgcgccatg ctttagtgat tgcgtctgcg
      961 ccgaagctgt ccacgcaaaa tatcgcgttc aacgatgtgg cgcgtcaggc gattttgacc
     1021 gaccccgatt tcaacgaagg acattaccgc agccgtaaca ccgttcctgc acggggctta
     1081 cggattgccc gcatgatggg acacattacc tatcttgccg aagacggttt gggcaaaaaa
     1141 ttcggacgcg atttgcgctc caatggctat caatacggct atggcgttga atttgaagta
     1201 gaatcctatc tgcgctatca aggcgacaaa ttcgtcggac ggtttgatgc caacacctac
     1261 ctgctgatga ccaaagcttt ggactatttc gatccggcgg cggatttcgg caacagcctg
     1321 acccgcgccg tgcaggatgt gcaggcaaaa ttctttgtcg ccagcttcag caccgattgg
     1381 cgtttcgcgc ccgaacgttc gcacgaactg gtcaaggcac tgattgccgc acaaaaatcc
     1441 gtgcagtata tcgaagtcaa atccgcacac gggcacgatg cctttttaat ggaagacgaa
     1501 gcctatatgc gtgcggtcgc cgcctatatg aacaacgttt ataaggaatg tcagcaatga
     1561 acctgcgcga tgatttgcaa ctgatttacg accggatacc cgaaggcagc cgcgtcttgg
     1621 acttgggctg cggcgacggc gaactgcttg ccgccttggt cgaacacaaa aaatgcagcg
     1681 gctacggcat cgaaatcgac accgacagcg tgattgccgc gatgtcgcgc ggcgtgaatg
     1741 ttatccaagc cgatttggaa gaaggtttaa ccgcattcaa cgatcaaagt tttgatgtga
     1801 ttgtgttgag ccaaaccatc caagcgatgc agaataccga aaaaatcctg cgctgcctga
     1861 tgcgcgttgc caaacaggcg attgtcagct tcccgaattt cggctactgg cgcaaccgcg
     1921 tccaaatcgc actcggcgga catatgccgg tttccgaacg tatgccctac cattggtacg
     1981 acacgcccaa tatccattgg tgcacgctca aagactttga tttattgtgc gccaaaaaca
     2041 acatccgcat cctcgagcgc gccgtgatga cgggcaacag acaggtcaaa catttcccca
     2101 accttttggg cagtcttgca ttttaccgcg tcgggtaaga cagattgaga accttgtcgg
     2161 cattaacagg ttcttcggtt ctattaacaa caggccgtct gaaaaatgaa acggtaggtt
     2221 ttcagacggc ctctttaata ctggaaacaa tagaatgaaa aacaaaacct catcacttct
     2281 cttatggctt gccgcaatga tgctgaccgc gtgttccccg agcaaagaag ataaaacgaa
     2341 agaaaacggc gcatccgccg cctcgtccac ggcatccgcc gcttcgtctt ccgcgcccca
     2401 aaccgatttg caaccggccg catccgcccc tgataacgtc aagcaggcag aaagcgtgcc
     2461 gccgtcaaat tgcaccgacc tgcaccccgc caccggcatt gacgatctca tgcagcaaat
     2521 cgccgaacac attgactcgg actgtctgtt tgccctttcc catcacgaac tggaaacccg
     2581 tttcggctta cccggcggcg gctatgacaa catacagcgg ctgctgtttc ccgacatccg
     2641 ccctgaagat cccgactacc atcagaaaat catactggca attgaagact tgcgttacgg
     2701 aaagcgcacg atcagccggc aggcacaaga tgccttgatg gaacaggaac gccgcctccg
     2761 agaagcgacg ctgttgctga tacagggcag tcaagaaacc cgcggacaag gcgaggagcc
     2821 gaaacgcacg cgttattttg aagtttcggc aacccctgcc tattcgagcc ggcacaacaa
     2881 cggacttggc ggcaatttcc aatacatcgg ccaattgccc ggctatctga aaatacacgg
     2941 agaaatgctt gaaaaccaat cactcttccg gctgtccaac cgtgaacgca atcccgacaa
     3001 accgttttta gacatccatt ttgacgaaaa tggcaaaatc acgcgtattg tcgtttacga
     3061 aaaaaacatc tacttcaatc caaacttggg gcgaagataa aaaaatgccg tctgaaacgt
     3121 tttcagacgg catttttcaa tgcggcaaat caagcgcgtt tgcggaattc gccggtgcgg
     3181 gtatcgattt cgattttgtc gccgttttcg atgtaagaca tcacttggat ttcggtgccc
     3241 ccgaccaggc gcgcggtttt catcactttg cctgaagtat cgcctttgac ggcaggctcg
     3301 gtatactcga cttcgcgtac gatgatggtg ggcagttcga cagaaatggg gttgccttcg
     3361 tagaaggtaa cttcgcattg gtcttccata ccgtcaacga tgaatttcaa cgcgtcgccg
     3421 atgttgtcgg cttcgatttc gtattggttg aattcttcat ccataaagac gtacatcggg
     3481 tcggcaaagt agctgtacgt acagtttttg cgggacagga tgaccacgtc gaatttgtcg
     3541 tcggctttgt aaatggtttc ggaagccgcg ccggtcagca ggtttttcag tttcatgctg
     3601 actttggcgg aagagcggcc gcctttgatg tattcggttt tttgaacgac cataggatcg
     3661 ttgccgacca taaatacatt gccggcgcgc agttcttgtg ctgttttcat tgtgattttc
     3721 caatcaaatc ggttgtcgta aaacgcacta ttctagcgta ttttttgatg ctttgaaaca
     3781 aaggcggcga gtttttcgga tgcggaaggc tgcccgaaaa gataacggct ccaatcctcc
     3841 gcgccttgcc gccagccgtt ttgatgttgt tgcaggattt gccaacattc gaggcgttgc
     3901 gtggcggata aagcccctcc gccgtttagg tcgtctgaaa ggcagcggtg tgccgatgcg
     3961 gtttcgggcg tgtagaaacc gtgtgcctta tcccaaaagg cgtggagttt gtcgagatgg
     4021 acattctcgt cttgcggata gatgtgccaa aagaagggtt ttccggcaag ctgggcgcgc
     4081 acgaaactgt cttcgccgcg gatgacggcg cagtcggcaa ggtgcagcag tttgtcgaag
     4141 tcctgttgcg gcacgaaagg gattttgacg aggcggacgg atgccgtctg aaaaacatcg
     4201 ccgtcgtttt gcagggcatc ttgcggaata acgccgtttt gtttgaggct gtcgataatt
     4261 tgcgcccccg ccagcaacag tgtcaacgga ctgcctgcct gtcgccacat ttccagccac
     4321 tttgcccaaa catcgctccg atagccgaaa agcagccatt cgggggcgtt tttttcggga
     4381 agcatcagcc tcttgcgcaa ggctccgcta tcgaaacgga cggcttcgca gtaatcacgt
     4441 tcgcgtatca acccgccgct tttttcgctg aaacccataa accaaaaata tttttgaaca
     4501 ccctcctgcg gcgaaggcat cagatgcagc ctttcattgc tttcctccgc gctcaaatat
     4561 tcccaattca gccaaagcgg cttgtgtcgg cggataatgt gcagcacatt ttcgggcagg
     4621 tcgcaggcaa aagtttcgat gacgacatcg ggaacaggcg cggtatcaat atctgccgca
     4681 tcggaatgcc aagtgcggac atgaatatcc tgatgaacgc agggaacatc gggcaaatca
     4741 gggcaaagcg cacgcaaggc ggacacatcg tccgtccaca aatgcacctg ccaaccgagt
     4801 tcgcggtgca aaacacgggc gagccgccac gaaacgccga tgtcgccgaa attgtcgatg
     4861 accttgcaaa aaatccaaca gacaaaagga ggagtattca tagaaataac ggaaacaagc
     4921 taaaacgcgc cgaaatagtg gaacaaatac acaaccagca aaacaatcgg aataaacagc
     4981 aacatggttt gacgtttcaa atcaaactct tgtttcaaat gatgcttgat ggtgtcgaaa
     5041 aggacgaatt tgaacaaaaa cgtcgcttcg caggcaagga tgatgccgtt gatcagatgc
     5101 tggggtttgt cttcgggcgg attggcgacg aaaacggcaa gtgtcgccaa agggatgagg
     5161 cagacgacaa gccagcggac aagcaggttt cgggacattg caaggctttc aaaaaagcaa
     5221 aacgccatta tcgccccaag cgcacgcctt gtctaacgga cgcgccggcg atgccgtctg
     5281 aagccttgcc gcaggaaaac gcagtgcttc cgcaaagcaa aatataaggg cggcgcgctt
     5341 tccgctataa tccgcatttt cgcaaccctg aaacatcatg ccgaccccga aagcctttgc
     5401 cctgctcggg ccgaccgccg gaggcaaaac cgcgctcgcc ctcaaaattg ccgaaaccct
     5461 gcctgtcgaa atcatcagcc tcgattccgc gctggtttac cgcgatatgg acatcggcac
     5521 ggcgaaaccg tccgcctccg aacgcgcctt tgtcccgcac caccttatcg acatcatccc
     5581 gcctaccgaa tcatacagcg ccgcccgctt tgtcgaagac tgcacgcgct tggtcgggga
     5641 aatttcctca cgcggcaggt ttgcccttat tgtcggcggc acgatgatgt atttccgcgc
     5701 cctgacccaa ggtttgaacg atttgcccga agccgacgcc tgcctgcgtg ccgacttgga
     5761 cgaacaaaaa cagatgtacg gcttggattt cctctaccgc accctgcaaa aagtcgaccc
     5821 tgaaaccgca tgccgtctga aaccgaacga cagccagcgt atcgaacgcg ctttggaagt
     5881 ttattatctg accggcaaac cgatgagcga acacctcggc agacaaagcc cccatacgct
     5941 ccccttcgat ttgcataccg ccgccctgat tcccgaaaac cgcgcccgcc tgcatgaaaa
     6001 catcgccctg cgtttccacc tgatgcttga acagggcttt atcggcgaag tagaaaacct
     6061 gcgccgccgc tatcccggcc tgaccgccga ctcccccgcc atccgctgcg tcggctaccg
     6121 tcaggcatgg aaatatctgg atggaaaaac cgatttcccg gcatttgtcg agaaaggcat
     6181 tgccgccacc cgccaacttg ccaaacgcca actgacttgg ttgcgcaaaa cacctttaga
     6241 ctgcgttgcc gacccgtttt cagacggcac ttcatgcaca cgcctgattg aggcggcaaa
     6301 acggtttttc ggcgcataaa ccatgctgac cacaccgccg ctcgccccca aaaccgtcgc
     6361 cgccctgcac aggctcggta tccgtacgct cgaagaactg cggcaaaacg gttcggtcaa
     6421 agcctttctg ctgctcaaag catcgggttt gaccctcacg aaaagcacgc tgtggcagct
     6481 tgagtcgctg ctgaacggca cactcccgca agaaatcacg caacacgaca aaacgcgcct
     6541 ttttgccgaa ttgaaaaacc atccgcccgt cgccgccttt ccgccgcagg aagaaatgga
     6601 acacttcatg cgcgaagcac tgcgccaagc agaacaatcc gccgctgacg gcgaaatacc
     6661 cgtcggggca gtcatcgttt cagacggcaa aatcatcgca tcggcacaca acacctgcat
     6721 tgccgactgc aatgtcagct gccacgccga aatcaacgcc ttggcacagg caggcagcga
     6781 aatgcaaaac taccgccttg acggatgcga catctacatc accctcgaac catgcgccat
     6841 gtgcgcgtcc gcactgatac aggcacgaat caggcgcgtg atctacggtg cggcagaacc
     6901 caaaaccggc gcggcaggaa gcatcgtcaa cctgtttgcc gacaaacgcc tcaatacgca
     6961 caccgccata cggggcggaa tcctgcaaga agaatgccgc gccgtattaa accgtttttt
     7021 ccaaaacaaa agaaaaggtt gaacctatga ccgccttgct cgtcatcctc gccctcgccc
     7081 tgatagccgc cggtacggcg ggcatcgttt accccgccct gcccggattg gcattgatgt
     7141 ttgccggaac ctggctgctc gcctactccg gcggctacca aatctacggc gcgggcgttt
     7201 tgtggacggt cggactcatc agccttgccg gcatactggc ggactatgtg gcaggcatat
     7261 gggggacaaa atataccgga gcgggcaagc tcgccgttcg cggcgcattg gtcggcagca
     7321 tcatcggcat atttttctcc cttcccggac taatactcgg tccctttatc ggcgcggcgg
     7381 caggcgaact gatcgatcgg cgcaatatgc ttcaggcagg taaagcgggc ttgggtacgc
     7441 tgttggggct tatcgtcggt acgacattca aaatcggctg cgccgtatcc atcttgttta
     7501 tcctgttggt gaaatacatc gcctacctgt tttaacccct tcagacggca taccggcaaa
     7561 ttgctataat gccgtctgaa cccttttcac aaagcaccat atggcaaacc aaagacctat
     7621 ctacggcttc catgccgtca acgcccgatt gtggcaaaac cccaaatcca tcgtcgaact
     7681 ctatgtccaa gaaggcaaaa acgatgtacg cacgcgcgaa gtgttggaaa aggcggcaaa
     7741 cgaaaacatc cgcgtatatt ttgccgatgc cgaccgcctc aacgccatca gcaaaggtgc
     7801 gcgccatcag ggcgtggtcg gatttatcga tgcctccaaa aaccacgtcc acctcgaaga
     7861 cgtattggaa aacttaagcg aaccgccgct gctgctgata ctcgacggca tcaccgatcc
     7921 gcacaacctc ggcgcgtgcc tgcgtaccgc cgacgcaatg ggcgtacatg ccgtcatcgc
     7981 accgaaagac aaaagcgcgg ggctgaacgc caccgtcagc aaagtcgcca gcggcgcggc
     8041 ggaaaccgtc ccctacatca ccgtaaccaa cctcgcccgc accctgcgcg agctgaaaga
     8101 atacggcatt tggatcatcg gcaccgatat gggcggcaac gccgaccttt accattgcga
     8161 tctgcccgac agcgcggcat gggtgatggg caacgaaggc gacggtatgc gccgcctgac
     8221 acgcgaacat tgcgacatgc tggtgtccat acccatgttc ggcacggtcg aaagcatgaa
     8281 cgtctccgtg tctgccggaa tggtattaag cgaaacccgc cggcagcgcg tattaaaaaa
     8341 cgaaaaagcg taagcctcaa aaacaatgcc gtctgaaaca gggattctgt ttcagacggc
     8401 attcgttttc aaaatcagtg ttccaagtgg tcggtatcaa acttgaccgc cgcctcggtt
     8461 ttatcttcgg gcaagaccaa attcagcaag actgccgtaa tgccgccggc ggaaatagag
     8521 ttttggaaca agacaggcag gtttttaaac acttccggct caaacgccac gcccaagccc
     8581 aagccgaccg aagtcgccgc aatcaccgcc tcgcgcctgc ggatgccgtg gctgaccaaa
     8641 atccgcacac ccgcaatcgc aatcaagccg aacatcaaaa ccatcgcgcc gcctaaaacc
     8701 ggactcggaa tcgtcgtaaa cgcgcgtccg acaacgggga acagacccaa cagcaccaaa
     8761 atcatggcaa tatatttgcc cacatggcgc gaagccacgc cggtcatctg aatcacgccg
     8821 ttgttttgcg caaacgtcgt cagcggcagc gaacccaaag ccgtcgcaat cactgacacc
     8881 aagccgtcag ccaacacgcc gccgcgcagg cgtttggtgt attcctcgcc ttcaatcggc
     8941 tggtcggaca ccattgccgt cgccgtcaaa tcgccgaccg cctcaaacac gctcaacaag
     9001 aaaatcgcac ccgccacaat aaatgcgtgc cagtcaaaag caaaaccata tttaaacggt
     9061 acgggcagcg taaccagcgg caggttttgc agtgccgaaa aatccacctt gcccaaaaac
     9121 agcgcgacga tatagccggc aatcagaccg accgcaatgc cgctcatccg cagcagcggg
     9181 tttttcatgc agttgaacac cagcacaatc agcagcacca gcgatgccag ccctaagttt
     9241 tccatcgagc cgaacgtgcc gtccgccttc gcgccgaagc cgccgccgaa atcggtaatg
     9301 ccgacgtgta ccaaactcaa accaatgagc atcacgacca cgccgctgac cgtcggcgta
     9361 atcacttttt tcaaatacgg cagaagccac gccgagaaac acaccaaaaa cgcgccgaca
     9421 aacgatacgc ccaagagcgt cgaaatcatc gcatccttag tcaaaccgcc ctctttcatc
     9481 cccgcgccca gcgcaatcat gacggtaacg aacgagaaat tcaccgactg gatggacagc
     9541 atccccgaac cgaccggccc gaagcggttg acctgcaaat aagtgccgac acccgacgca
     9601 accatcgcca tcgacacgag atacgccgtc atctccaccg gcagttccag cgcgccgccc
     9661 acaatcagcg cgggcgtaat catcggcacg aaaatcgcca aaagatgggt aaccgcgctc
     9721 aacagcgcat taccgaacgg cggcctatcc tccaaaccgt acaccaaatc aggcgcatcc
     9781 gccccttttc ccatatgacc gctcataatg tttccttgct tcaaatgttg aaaaaaccaa
     9841 agcgcggatt gtagcgaatc cgccataccg cttcaatttc gatttcggac ggcatacgcg
     9901 cccgtccggc aaacagattc cgaaccgtcg ccgccggaat atcgtttaca aaacgcaata
     9961 acctgatttc aaacacaaaa atacaaaccc gacgcatccg ccgtcaagcc gccccgctca
    10021 tcccggaccc gccggatttc acacctgcgg taaagtaggc tacaatcgga agcattaccg
    10081 ccccctacac cacaactgat aaaaggttct gtatgttaca aggtagcctg gttgccctga
    10141 ttaccccgat gaatcaagac ggcagcatcc attacgaaca actccgcgac ttaatcgact
    10201 ggcacattga aaacggcacg gacggcatcg ttgccgtcgg cacgacaggc gaatccgcca
    10261 ccctctccgt cgaagaacac accgccgtca tcgaagccgt cgtcaaacac gttgccaaac
    10321 gcgtccccgt catcgccggc acaggggcaa acaacaccgt cgaagccatc gccctttccc
    10381 aagccgccga aaaagccggg gcggactaca ccctctccgt cgtcccctat tacaacaaac
    10441 cttcgcaaga aggcatgtac cgccatttca aagccgttgc cgaagccgcc gcgattccga
    10501 tgatactcta caacgtaccc ggcagaaccg tcgtcagcat gaacaacgaa accatcctgc
    10561 gccttgccga aatccccaac atcgtcggcg tgaaagaagc cagcggcaac atcggcagca
    10621 acatcgaact catcaaccgc gcgcccgaag gcttcgtcgt cctttccggc gacgaccata
    10681 ccgccctgcc gttcatgctg tgcggcggac acggcgtgat taccgtagcc gccaacgccg
    10741 cgcccaagct ctttgccgat atgtgccgcg ccgcgctgca agtcgacatt gccctcgcac
    10801 gcgaactcaa caaccggctg attccgattt acgacaccat gttctgcgaa cccagccccg
    10861 ccgcaccgaa atgggcggta tccgccttgg gcagatgcga accgcacgtc cgtttgccgc
    10921 tcgttccgct gacggaaaac ggacaagcaa aagtccgcgc cgccctgaaa gcctcaggac
    10981 aactttaaat atagccatgc cgtccgaacc gttcggacgg cataacgcaa caaacacttt
    11041 aatatccatc acacaggatg acacgatgac ccatatcaaa cccgtcattg ccgcgctcgc
    11101 actcatcggg cttgccgcct gctccggcag caaaaccgaa cagcccaagc tcgactacca
    11161 aagccggtcg caccgcctga tcaaacttga agtcccacct gatttgaaca accccgacca
    11221 aggcaacctc taccgcctgc ctgccggttc gggcgccgtc cgcgccagcg atttggaaaa
    11281 acgccgcaca cccgccgtcc aacagcctgc cgatgccgaa gtattgaaaa gcgtcaaagg
    11341 tgtccgcctc gagcgcgacg gcagccaacg ctggctcgtt gtcgacggca agtctcctgc
    11401 cgaaatctgg ccgctcctga aagccttttg gcaggaaaac ggcttcgaca tcaaatccga
    11461 agaacccgcc atcggacaaa tggaaaccga gtgggcggaa aaccgcgcca aaatccccca
    11521 agacagcttg cgccgcctct tcgacaaagt cggcttgggc ggcatctact ccaccggcga
    11581 gcgcgacaaa ttcatcgtcc gtatcgaaca gggcaaaaac ggcgtttccg acatcttctt
    11641 cgcccacaaa gccatgaaag aagtgtacgg cggcaaagac aaagacacga ccgtatggca
    11701 gccctccccg tccgatccca acctcgaagc cgctttcctg acgcgcttta tgcaatattt
    11761 gggcgttgac ggacagcagg cggaaaacgc atcggcaaaa aaacctaccc ttcccgccgc
    11821 caacgaaatg gcgcgtatcg aaggcaaaag cctgattgtc tttggcgact acggcagaaa
    11881 ctggcggcgc accgtgctcg ccctcgaccg catcgggctg accgtcgtcg gtcaaaacac
    11941 cgaacgccac gccttcctgg ttcaaaaagc cccgaacgaa agcaatgcag ttaccgaaca
    12001 aaaacccggc ctgttcaaac gcctgctggg caaaggcaaa gcggagaaac ctgccgaaca
    12061 gccggaactg attgtctatg cagaacctgt cgccaacggc tcgcgcatcg tcctgctcaa
    12121 caaagacggc agcgcatatg ccggcaaaga cgcatccgca ttattgggca aactccattc
    12181 cgaactgcgt taaaccattc aaaacaaaag cagcttccaa acagaagctg cttttttatg
    12241 cggaactttt tacaacaaat ggggcaaaat atcctcaact gcctgaacca acgcatccac
    12301 caaaggcgga tcgaacgcac gatgccctgc ctgaaccacc ctcaattccg cttcgggaaa
    12361 ggctttcgac agcgcccacg cactctgcat cggcgtacac aaatcatacc gcccctgcac
    12421 gataatggtc gggatatgcc gtattttgcc gatattgttc aaaatcgccc tatcgccctg
    12481 caaccagccg ccgttgacaa aataatggtt ttccaaacgc gcaatcgcca gtgaggcata
    12541 agcatcttca tccacatcct ccggctcgaa acggatcaga tagctttccc aatccgccca
    12601 agccttcgcg gcagacaggc acacttcttc atcttgatga aacagcaacc cgtgatacgc
    12661 ctcaatcagc cggttccgcc gattttcagc aatcggcgcg acaaattttt gccattgttc
    12721 cggataaatc cggctcacac cgcccgcctc gttcagccac gccgtttcag acggcctgca
    12781 caaaaatatc ccgcgcaata ccaatccctt tacccgttca ggatgggttt gggcataagc
    12841 cagcgacaaa gtgctgcccc acgaaccgcc gaacaccagc catttcctga tacccagcat
    12901 ttcacggact ttttcaatat ccgccaccaa atcccaagtc gtattgtctt ccgcacaagc
    12961 atacgggcgc gaacgtccgc aaccgcgctg gtcgatgatg acgatacgga acacatcggg
    13021 attgaaaaaa ccccgacatt caggcgaagc ccccgcgccc ggcccgccat gtaaaaaaat
    13081 gaccggcaca ccgtcgggat tgcccgactc ttcccaataa atttgatgaa tttcagacac
    13141 ctgcaagtat ccgctatgaa aaggctgttt tatctcatac atcatcttct cccatattgc
    13201 ttctgacaat ccgaacattc ccacacgcaa catcacgcac gagacacaag ccgccccgaa
    13261 aggcaaatgc cgaaaaatcg cgatatagtg aattaacaaa aatcaggaca aggcggcgag
    13321 ccgcagacag tacggatagt acggaaccga ttcactcgtg cttaagcacc ttagagaatc
    13381 attctctttg agctaaggcg aggcaacgcc gtactggttt ttgttaatcc actataacag
    13441 caaccctgtc g
//
LOCUS       DAXHNE010000040        13389 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.040, whole genome shotgun sequence.
ACCESSION   DAXHNE010000040 DAXHNE010000000
VERSION     DAXHNE010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13389
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.040"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(111..539)
                     /locus_tag="ACFM4Z_000897"
     CDS             complement(111..539)
                     /locus_tag="ACFM4Z_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HFC6020959.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(604..2352)
                     /locus_tag="ACFM4Z_000898"
     CDS             complement(604..2352)
                     /locus_tag="ACFM4Z_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HFC6020960.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTLMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2679..4112
                     /gene="ccoN"
                     /locus_tag="ACFM4Z_000899"
     CDS             2679..4112
                     /gene="ccoN"
                     /locus_tag="ACFM4Z_000899"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045278 - plasma membrane respiratory
                     chain complex IV [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HFC6020961.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4139..4750
                     /gene="ccoO"
                     /locus_tag="ACFM4Z_000900"
     CDS             4139..4750
                     /gene="ccoO"
                     /locus_tag="ACFM4Z_000900"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HFC6020962.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4755..4925
                     /locus_tag="ACFM4Z_000901"
     CDS             4755..4925
                     /locus_tag="ACFM4Z_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HFC6020963.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4949..5860
                     /gene="ccoP"
                     /locus_tag="ACFM4Z_000902"
                     /pseudo
     CDS             4949..5860
                     /gene="ccoP"
                     /locus_tag="ACFM4Z_000902"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
     gene            5936..6280
                     /locus_tag="ACFM4Z_000903"
                     /pseudo
     CDS             5936..6280
                     /locus_tag="ACFM4Z_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
     gene            6482..7891
                     /locus_tag="ACFM4Z_000904"
     CDS             6482..7891
                     /locus_tag="ACFM4Z_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HFC6020964.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYTMDEIHSDMMLGAAGDYLLETAASL
                     TIIMVVSGLYLWWVKRRGIKAMLLPSKGRARSWWRNLHGTFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7993..11202
                     /gene="recC"
                     /locus_tag="ACFM4Z_000905"
     CDS             7993..11202
                     /gene="recC"
                     /locus_tag="ACFM4Z_000905"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFC6020965.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSALQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRRLG
                     LDDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSEMAGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPKGGNPMWQDVSAWYADV
                     NQTAMFGRFAAFLETLTDIVRIWRQPATVGEWVARCRDLLETLFQAEADDQKSVQNLE
                     NEWVKWQAETELAQFSGQLPPHTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNLIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAEIYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQDFQTAAKQIDTAVLNSPKLPPLSYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLATAEAFAQKQDWEAALKKAQTAYHGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLVRLLADTFAAAEKKEEAGAA"
     gene            complement(11272..12840)
                     /gene="mtrF"
                     /locus_tag="ACFM4Z_000906"
     CDS             complement(11272..12840)
                     /gene="mtrF"
                     /locus_tag="ACFM4Z_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HFC6020966.1"
                     /translation="MNQTDTRRGGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFGLSVPDPRPVGAKGRADDGLIYVVSLLNADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTLYPAP"
     gene            13172..13384
                     /locus_tag="ACFM4Z_000907"
     CDS             13172..13384
                     /locus_tag="ACFM4Z_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020967.1"
                     /translation="MKNEIQKIMDKYNPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
BASE COUNT         3348 a         3232 c         3558 g         3251 t
ORIGIN      
        1 cactgtaaaa acggagaagg acagaggacg gcagtaaggt ttgccgtcct ctttttcaag
       61 ttgcggtgga atgccgcttg aaggtgtggt ttaaacgttt cggcttgtaa tcagacgcgg
      121 gcgttgtcca gccgctctct gaagttttcc aaatttacgc ctgccgcttg ggcgcggcgg
      181 atgatttcgt caaccggcat accttctgcc gcccggcgga agccccacag gagggagcag
      241 cgcgtaccgg tccggcaata ggcaaggacg ggatattcgg cttgtccgat gagttggcgg
      301 aaggtttcga catcgtgttt ttggatgtcg cgtgcggtaa cgggttggtg atggaatcca
      361 gtaacgcctg cttgttccag ccactgtttg atttgggcga agtcgggttg cgattcttct
      421 tcgcggtcgg ggcggttgca gatgacggtt ttgatgccca gttgcgcgat ttgttccgca
      481 tcggctttgg tcagttgcgg agaaatatag aggtgttcgt caagtttcag aatggccatt
      541 ttgtttcctt tactttgcgg ttgtttttca gacggcattt tgtgtgcaat gccgtctgaa
      601 agtttaaccg aacaaccagt tgtccaacag gttccggtct gtgatatgta ggatggcagt
      661 atccgctgcc gctgcctgaa ccgtcaagac ggtttgacgc aatatgccgg cacggaagaa
      721 gtggatattg atttttgcat tgacgtggta tcgggcccat tgtgcggtaa agtcggtgca
      781 ggcataaccg tctaaagcaa tgattttgtc ttgcgggcac agtgccgcag attccgcgct
      841 gccgccgttg aagacatggg tcaggacgat gtggtcggtg ttttgtttga aacgtgcgcc
      901 aaaatcgcct gccgacggga cggggcagat gtgttctgcg tatccgccgc cgtgttgtcg
      961 gggaagcggc aggaaggtca gtcccacgcc tgcggttgcc aggcattcgg caagcggcaa
     1021 atcttcggta ctgtataacg ctttttggaa aaaatcttcc aaatccaagc cggtaatttc
     1081 ctgacagcgg atttgccagt gtttttccgg aatacccgag tgtgtgtccc tccactcccg
     1141 atagagtttg tccattaacg tatcgagaga atgtctgccg ttgcttcggt tgcgtattat
     1201 cagatcaagg cacaatgcgg caagcgcgcc tttctggtag tagctgacga tggcgttggg
     1261 gctgttttca tccggtttgt aaaatttgtt ccacgcggta aaactcgatt ccgccaaggt
     1321 ctgcctcaaa cggccgcggg tttgttgtac gcgcgtaatg ccttgtgcca gcaggtttaa
     1381 ataagattcg ggcgagatgg tgcggctgcg tgccaaaaac aaatcgtcgt aataggatgt
     1441 aataccttcg aatgcccata gttgttcggt atagttttct ttgtcgaggt cataagggac
     1501 gaacgcggca ggtttgatgg atttgacgtt ccacgcgtga aaatattcgt gggagaaaag
     1561 tccgagcaat gtggtgtagg tatcgtcggc atcggtcata ccgtacggcg gaaggctgtg
     1621 gcggtcggcg agcagggcgg tgctgtcggt gtgttccaaa ccgccgtaaa tatggtcgcc
     1681 gacgtggagc aggaacaaat atttttgaaa cggggcaggg gaggaaaaca ccgccagttc
     1741 tgtttcgcag atttttttga tatccgaaac cagcctgttg cggtcgaaat cgggatatat
     1801 gccgcttaag gcaattgtgt gcggaatgcc tgccgcctca aaatctaaaa attcaatcaa
     1861 gcccatctcg acaggtcggt caatcaattc ggcataagat gccgcctgaa agacaaacct
     1921 ccctgtttcc ggcagcgttg tggcaatacg ccattcggac ggaatacccg tcaattccaa
     1981 gcggtgcggc agcgtttccg ttccttcgac tttcaaaaac aggcacgatc cgtcaaaaaa
     2041 accgcgttct gtcgtcagga aagaacctcg aaccgacaaa tcgaatgcat atacggtgta
     2101 gcggatttgc cactcgccgc gtacggcggc ggcatgccag cggtttttgg caatttgttc
     2161 gagcggcatg gacgtgccgt tacaggatgc atggatagaa gtgatgtggc gggaaaaatc
     2221 ccgaatcaga tagctgcccg gaacccagtt tggcaggctt atttctatag gaagatcatc
     2281 atcttgtgtg aatgtcagca ggatgtgcca ctcgtggtcg agaggggacg gggttatttt
     2341 ataatgaatc ataactatca aatatatcgt taaacgggat ggattaaata cctttaaaat
     2401 gcagcttaac ctacatcaat aaagtgccac aaaaagggaa aattggaaac aaaagcaatg
     2461 gcggtcgggt tgtaatttat tataacaata tgatttatat gtaataaaaa acagattaag
     2521 caaagggctc gtcggcggcg cgaatgtaat tccgcttggc gtaataaggc agatatgtta
     2581 aatttagaaa aaattacgct tgcccggatt gcaccttaaa caggctgcgg aagcggcagg
     2641 ataaaaatct ttattcccat aaattcatgg agactctcat ggacacacaa acttacaact
     2701 acaaagtggt gcgccagttc gccatcatga ctgtagtttg ggggattgtg ggtatgttgg
     2761 tcggcgttat cgtcgccgcc cagctttttg ctcctgccct cgacttgtct aatatcggac
     2821 cttggttcca cttcggccgc ctgcgtccgc tgcacaccaa tgcggttatt ttcgcatttg
     2881 gcggttgcgg cctgatcggc acatcatact acgttgtcca acgtacttgt aatacccgtc
     2941 ttttcggcgg ttggctgccg gcatttacct tttggggctg gcaggcggta atcgttgccg
     3001 ccgtcgtcag cttccctatg ggttggaccc aaggtaagga atatgccgaa ctggaatggc
     3061 cgatcgatat tctgattact ttggtttggg tggcttacgc catcgtattc ttcggtacga
     3121 ttgccaaacg taagattaaa catatttacg ttgccaactg gttctacggc ggctttattt
     3181 tggcggtcgc acttttacac atcgtcaaca atatcagcat cccggccggt ttgatgaagt
     3241 cataccccgt ctattccggt gcgattgatg ctatggttca atggtggtac gggcataatg
     3301 ccgtgggctt cttcctgact gccggcttct tgggtatgat gtactatttc gtacccaaac
     3361 aagcagcccg ccccgtttac tcctaccgcc tgtccgtcgt tcacttctgg gcgttgattt
     3421 ttacctatat gtgggcgggt ccgcaccatc ttcactacac tgcgctgcct gactggacgc
     3481 aatctttggg tatggttctg tctttgattc tgttcgcacc ctcttggggc ggtatgatta
     3541 acggcatcat gaccttgtcc ggcgcgtggg acaaactgcg tacagacccg attcttaaat
     3601 tcctgattgt atccttgtcc ttctacggta tgtctacctt tgaaggcccg atgatgtcga
     3661 ttaaaacggt caatgcattg agccactata cggactggac cgtcgcgcac gttcatgcgg
     3721 gtgcgttggg ctgggtaggc tttgtaacca tcggttccgt ctattacatg attccccgtc
     3781 tgttcggcaa agaacagatg cacagcacca agctggtaga agcacatttt tggattgcga
     3841 ccatcggcgt ggttctgtat atcgctgcca tgtggattgc cggtgtgatg cagggtttga
     3901 tgtggagttc tttgaacgat gacggtacgc tgacctactc gtttgtcgaa tccgtaaaac
     3961 gcaccatgcc ttactacgtg atccgttttg caggtggttt attgtatctg agtggtatgt
     4021 gcattatggc gtacaacgtg taccgcacag ccatcggtgg taaagcagtc gatgccgaaa
     4081 tccctgcggt ttcccaaaca cagcaccact aaaatactaa gaaagatagg ctaccaaaat
     4141 gaaattacaa caattggctg aagaaaaaat cggcgttctg attgtgttca cgctgcttgt
     4201 agtcagtgtc ggtctgttga ttgaagttgt gcccttggcc tttaccaagg cggcaacaca
     4261 gccggcgccg ggcgtgaagc cttacaatgc cctgcaggtt gccggacgcg atatttacat
     4321 ccgtgagggc tgttacaact gccactcgca aatgattcgt ccgttccgtg cggaaaccga
     4381 gcgttacggt cattactctg ttgccggaga gtcggtttac gaccatccgt tccaatgggg
     4441 ttccaaacgt accggtcctg atttggcacg tgtgggcggt cgctattccg acgaatggca
     4501 ccgtatccac ctgctgaatc cccgtgatgt cgtgcctgag tccaatatgc cggcattccc
     4561 gtggcttgca cgcaataaag tcgatgtcga tgcaaccgtt gccaacatga aggctttgcg
     4621 taaagtaggt actccttaca gtgatgagga aattgcgaaa gcacctgagg ctttggcaaa
     4681 caaatccgag ctggatgctg tagtcgccta tctgcaagga ttgggtctgg ctttgaaaaa
     4741 cgtaaggtaa catcatggat attaacggta ttcgtgctct cttcacggta tggatcttta
     4801 tctgtttcct gttggtactc tatatcgtct tcaacaggcg gaataagaaa aactacgata
     4861 atgccgcaaa cagcattttt gatgaaaacc aagatgcgca agataagaaa agcgaaaacc
     4921 gttaatattg tgataacgga gcaaaacaat gaacacaaca tcccaattta ccagtaattt
     4981 ctggaatata tatattgcag ttattgtctt actgagcttt atcgctttgg cttggctgct
     5041 gctgtctcaa aatgttgtca aacgtccgaa gaagggcgaa gaagtacaaa ctacgggtca
     5101 tgagtgggac ggcattgccg aatacgacaa tccgctgccc cgctggtggt tttggctgtg
     5161 tgttttgacg tggctgttcg gtatcggtta tttggttatg tatccgggtg tcggcgacta
     5221 caaaggtctg ctgaaatgga ccagccataa ccaatatgaa aaagaggtca aaaaagccga
     5281 tgagcaatac ggcaaactgt atgccaagtt tgcggatatg ccgattgaaa aagtggcaaa
     5341 agaccctcaa gccaagcaaa tcgcccaaaa cctgtttaac acttattgta tccagtgcca
     5401 cggctctgat gctaaaggct ctaaaggttt tccgaatctg accgatagcg attggttgtg
     5461 gggcggtgat cccgataaaa tccacgagac catcgaaaaa ggccgtgttg cgactatgcc
     5521 tgcctggggt cctgctttgg gcgaagaagg cgtgaaagac gttgcccatt atgtgatgtc
     5581 cctttcaaaa cccgaaggtc agtatgatga ggagcgtgcc gcgcgcggac aagccttgtt
     5641 cagcggtccg cctgccaact gtttcacttg ccacggcgat aagggacaag gtatccaagg
     5701 tttgggtccg aacctgactg atgacgtgtg gttgtggggc ggtacgcaaa aatccattat
     5761 cgaaaccatt accaacggtc gcagcagcca aatgcccgct tggggacatt tcttggataa
     5821 agacaaactg catattatga ctgcttatgt atggggtctt tccgataaag acggtaaagc
     5881 tccggtgaaa aaagccgagc ctgcaccgac tcccgcaccg gcggcagaac ccgcagcctc
     5941 tgctcctgca gaagcagcac aagccgtgtc cgaagccaaa cctgccgcag cagaacctaa
     6001 agccgaggaa aaagctgcac ctgctgccaa agcggacggc aaataggttt atgaaaccgt
     6061 ttgtgccgcc tgccatggca atgcgattcc gggtattccc catgtaggca tcaaagccga
     6121 ttgggccgac cgcatcaaaa aaggcaagga cacgttgcac aaacacgcga tcgaaggttt
     6181 caatacgatg cccgcaaaag gcggtcgcgg cgatttgagc gatgatgagg ttaaagctgc
     6241 ggttgactat atggtcaacc agtcaggcgg caaattctaa cttgactgag tttccgatat
     6301 tatcggttat gaaaatccag attcagtatt gaatctggat tttttgtttt aacgggcatc
     6361 cggttttaaa cgcagactgc ctatttgttg taatgaattt tgactttagt cataaagaat
     6421 tagtctttat tgtttataat cgcgcatcat cacgatagcc ctattttcaa aaggacgatt
     6481 aatggataca caaatcaaaa ctgaagccga caatcagagc aaccggcgtt atctgaccgt
     6541 ttggcggtgg catttttatg ccggtctgct ggttatgcct tttcttaccc tgcttgccgt
     6601 tacgggtttg ggtatgctgc tgtttgccaa tattaccggt aaggagggcg agcggattca
     6661 tgttgtgccg caggcaacgg tacaacctct gtctgttcag gcggaagcgg cacgcagtgc
     6721 cgttaatccg gaaacttcgt ccgtcgttca gtatattgcg ccgcgtgccg atgatatggt
     6781 tgccgtgttc cgtgtcaaca atgagggcaa agcaacgatg gtcgcggtcg atccttatac
     6841 ggcaaaagtg gtcagtacca tgccgcgcaa tcagggttgg tattacacga tggatgaaat
     6901 ccacagcgat atgatgctcg gtgcggcagg cgattatctt ttggaaacgg cagcttcact
     6961 gaccattatt atggttgtca gcggcttgta cctttggtgg gtgaaacggc gcggcatcaa
     7021 ggcgatgctg ctgccgtcaa aaggcagggc gcgttcttgg tggcggaatc tgcacggcac
     7081 gtttggaact tgggtgtcgt tgattttgct gttgttctgc ctgtcgggta ttgcttgggc
     7141 gggtatttgg ggcggcaagt tcgtacaggc ttggagtcag ttccctgccg gtaaatgggg
     7201 tgtcgaaccg aaccccgttt cagtcgtgcc gacccacggc gaggtattga atgacggcaa
     7261 ggttaaggaa gtgccgtggg ttttggagct tacgcctatg cctgtttcag ggacgactgt
     7321 gggcaaagac ggcattaacc ctgacgagcc gatgacattg gaaaccgtcg accgctttgc
     7381 gcgggaaatc ggtttcaaag ggcgttatca gttgaatttg cccaaaggcg aggacggcgt
     7441 atggactttg tcgcaggatt ctatgagtta cgacatgatc agcccgtttg ccgaccgcac
     7501 ggtacatatc gaccagtaca gcggcaaaat ccttgccgac atccgttttg acgattacaa
     7561 cccgttcggc aaatttatgg cggcaagcat tgcgctgcat atggggactc tgggctggtg
     7621 gagcgtgttg gcgaacgtct tgttctgcct tgccgtcatt tttatcggta tcagcggctg
     7681 cgtgatgtgg tggaaacgcc gtccgaccgg agcggtgggc atcgttccgc cggcgcagaa
     7741 agtcaagctg ccggtttggt ggatgatggc attgccgcta ttggcaatcg cactgctctt
     7801 cccgaccgca ctgcttgcca ttgccgtgat ttggctgttg gatacgctgc tgttgtcgcg
     7861 gattcctgtt ttgaggagat ggtttaaatg accaaatgcc gtctgaaagg ttcagacggc
     7921 attttgtttg aaggcggacg ggggaaaggc tatataatcc cgaatacttg accacagctt
     7981 ctgtttgaaa tcatgtttta tctgtatcaa tccaaccgtc ttgaaacgct ggcggcattg
     8041 tttgcccgca ttcaaaaagt caaaccgctg aaatcggctt tacagcccga acagattatt
     8101 gtgcagagtc aggggatgcg ccgctacctc aatacctgcc tcgcccgcga tttgggcgtg
     8161 gcggcgaatt tgtcgttcag cctgcccgcc ggcctgacgt ggaagctgat gaaaaaactg
     8221 attcccggta ttccggaact cagcccgttt gcgcccgaag tcatgcgctg gcggctgctg
     8281 gatttgttcc gcagcgaggc atttcggaat acggcagaat ttgaagatgt gaggaatgtg
     8341 ctgcaagact atctgggcag cggcgaatcg gcagattacc agcttgcggg acagcttgcg
     8401 gacatattcg accaatacct cgtgtaccgt cctcagtgga tagacgcttg gcagcagggc
     8461 aggcggctcg gtttggatga cgacgaaatc tggcagtcca aactgtggcg ttacctcgac
     8521 gacggcaggc agagcgcgcc gcaccgtgtc gcgttgtggg aaaagctgtt ggaatctttg
     8581 agcagtgata agctgcccga gcgttatttc gtgttcggca tttccacgat ggcgccgatg
     8641 tatttgcaac ttttgcacaa gctgtccgaa cattgcgacg tgttcgtgtt cgcactcaat
     8701 ccgagcggga tgtactgggg caatgtcatc gaagcggcgc aaatcctcaa aggtggcggc
     8761 gatcccgatt taactcaggc agggcatccg ctgctcgctt cattgggcaa gcaggggcgg
     8821 gactttttcg actttttgaa cgaaatggaa atagaaggcg aaacgccggt atttgaggaa
     8881 ggcgggcgcg atacgctttt acacgccctg caaaccgata ttcaaaacct gaaaatgccg
     8941 tctgaaatgg cgggaagcgt caacacgggc gacggctcga tacgcatcgt ctccgcacac
     9001 agccctttgc gcgaattgca gatactcaaa gacaagctgt tgaaaattct gcatgaacat
     9061 cccgattggc agccgcacga tatcgccgta ttaaccccga atatcgaatc ctatacgcct
     9121 tttatcgaag ccgtgttcgg acaggcgcag cccggtgcgc aggcattgcc gtattccgtg
     9181 tcagacgtga aaatcagccg ccgccaaccg ctgtttcatg ctttgtcatg cctgttcgac
     9241 ttgttggaaa gccgatttga agtcgataaa gtgcttgtgc ttttagaaac cgcgcccgtg
     9301 ttgcgccgtt tcggactgac tgaggacgat ttaccgcttt tgcacgacat ggttgccgat
     9361 ttgaacgtcc actggggttt ggacggagaa atgcgcggcg gcacggatca gctgttcacc
     9421 tggaagcagg cggtagaacg catgatattg ggctggatgc tgcccaaagg cggcaatccg
     9481 atgtggcagg atgtcagcgc gtggtatgcc gacgtgaatc aaaccgccat gttcggacgt
     9541 tttgccgcct tcctcgaaac cctgacggat attgtacgga tatggcggca gcccgcaacg
     9601 gtcggcgaat gggtggcgcg ttgccgggat ttgcttgaaa cattgttcca agctgaagcc
     9661 gatgaccaaa agtcagtcca aaaccttgaa aacgaatggg tcaaatggca ggcggaaacc
     9721 gaattggcgc aattttccgg acagttgccg ccgcacaccg tcatccgcca tatccgacgc
     9781 tttctcgaca gcgaaagcga ggcaggcttt ttacgcggcg gcatcacctt ttgcagtatg
     9841 gtgccgatgc ggagcctgcc gttcaaagtc atctgcctgt tgggtttgaa cgacggagat
     9901 tttccccgta ataccaaagc cgccgtattc gacctgattg ccaaacatcc cgccaaagga
     9961 gaccgcgccc gccgcgatga cgaccgctac ctgttcctcg aagccctcat cagcgcgcgt
    10021 gaaatcctct acctgtccta catcgggcgc gacatccgca aagacgaaga gcttgccccg
    10081 tcttccctgt tgggcgaact catcgatacc gttgccgcta tggcgggcat cggtagccgc
    10141 caacttgcac aaaacctgat agaacagcat ccgctgcaag ccttctcgcg ccgatatttt
    10201 caagaaggcg gacgttcaga cggcatattc ggcacgcgta ccgactacgc cgccgcgctc
    10261 ggacaaacgc ccgaaccgcc gcaacccttt ttcgatcaac ccgtagaaaa cgccgaacct
    10321 gttgccgaaa tcggacagga cgaatttatc cgcttctggc gcaaccctgt caaagtatgg
    10381 cttcagcagc agcttgcgtg gagcgaaccc catatcggcg aagcctggga gcctgccgaa
    10441 cccttcgagc cgcaacacgc cgatcaaatc gccgaaatct acatcgaagc acggcgcgaa
    10501 ggacgggatt ttgcccaaac cgccgcccgc atcggggcgg aaagcctcct gccgtcggga
    10561 gagttgggca gactttggca gcaggacttc caaactgctg ccaaacaaat cgacacggcg
    10621 gttttaaaca gccccaaact gccaccgctt tcatatgcca taccgtcgga cgggcaaatc
    10681 ctgaaaggca gcttgggcaa tctgtaccgc tgcggacaag tgttttacgc ctacggcaaa
    10741 cccaacgcgc cgcaacgtat cgcttttctg ctggaacacc tgatattttg cgccgttatg
    10801 ccgtctgaag ccgaaacgcg gcaaaccttt atcgtccaat ccggagaaac cgaaatattg
    10861 gcggaaatcg cgcaagacag ggcattacag ctattgtcgg aatggatggc gtttttcaat
    10921 atcgggcaaa accgcccgct gccgtttttt gccaaaacct cgcttgccac cgccgaagcg
    10981 tttgcccaaa aacaagattg ggaagccgcc ctgaaaaaag cccaaaccgc ctaccacggc
    11041 aacaaagtca gcaaagggca gaaagactat accgaagtcg ccctcgtgtt cggcaacgca
    11101 agccaaaacc cgctcgaaca gcccctgttt gaaaacctcg tccgtctgct tgccgacacg
    11161 tttgccgcag cggaaaaaaa ggaagaggcc ggagcagcct gacgggatgg aaaggcaaat
    11221 gccgtctgaa aacagcaaaa aggttttcag acggcatttt gtttatcgtg tttaaggtgc
    11281 gggatacaaa gtgggtgcgc cgggaccgac gggcagaccc aaaacaaata cccaaatgca
    11341 gaataaggca atccatgcaa ttaagaagaa agcggaatac ggcaacatca tagaaatcag
    11401 cgtacccacg cccgcatctt ttttgtattt gatcaccgtc gccataatca gcccgaaata
    11461 actcatcatc ggcgtaataa tattggtaac ggaatcaccg atgcggtaag cggcttgaat
    11521 gacttcgggc gcgtagccgg ccaacatcag catagggacg aaaatcggcg cagttaccgc
    11581 ccattgcgcg gaggcggagc ctatcatcag attgataaaa gcacaaatta aaataaaacc
    11641 gataaacaac acgctgccgc ccaagccgac ttcttttaag aacgtcgccc ctttaacggc
    11701 aatatattgc ccaatattcg tccaattaaa aaatgcgaca aactgtgcgg caaaaaagat
    11761 gatgaccaaa taaagcccca gagtactcat cgattcggcc atcgcattaa cgacttcctg
    11821 ttcgccgcgc aaacttcggg ttacccggcc ataaacaatg cccggcagcg caaacaacaa
    11881 gaaaataaaa acaacaatcg attttaaaaa cggcgaaccg gaaaccaatc ctgtttcagg
    11941 atgacgcaaa ataccgtcgg cagggacgat gctccaagcc aatagggcgg ataaggcaac
    12001 aaacaccacg ccagcccaaa ttaatccttt atattccaaa ggcgtgattt cattggaatg
    12061 ccgaatgtct ttttcttctt gtgacaaatc tgattgataa gggcccaatt gcggttcgac
    12121 gattttttca gtaacaaaat aaccaatcaa agcaatcaca aacgtactgg ctaccataaa
    12181 aaaccagttg gcttcagggc ctacgacgta gtcgggatgg atgatttgcg ccgcctgttg
    12241 ggtgatgcct gccaagagcg gatcgattgt gcctaagaac agattggccg aataaccgcc
    12301 cgaaacgccg gcgaaagccg cagccagacc ggcaagcgga tggcggccga gggaatgaaa
    12361 gatgatggcg gacaaaggga ttaggacgac atagcccaat tcagaagcgg tattagataa
    12421 aatccctgta aaaacaacca taaaagtagt gagtttgcgt ggagattttg tgagcaataa
    12481 gcgcattaat gcggaaatca agcccgattt ttccgcaatc cccacgccca ataaagaaac
    12541 caacaccgtt cccaacggcg cgaaaccggt gaaattttta acggtatgcg tcaggatttt
    12601 gatcaaaccg tcggcattga gcaggctgac aacgtaaatc aaaccgtcat cggcacgtcc
    12661 tttcgcacca acagggcgcg gatcggggac ggatagtccg aaatacgcac cggcggcaga
    12721 ggcaatcagc aataacacaa tgaaaataat aaaaagcgta accgggtgcg gcaacatatt
    12781 gcccagccat tcgactgtgc gtaaaaatcg tccgccccgc cgcgtatcgg tttgattcat
    12841 ctgtttctcc ttaaaaaaat atatcgtgtg cattaaattt ttgaatgtaa catgaagtgt
    12901 tttgtaagca aactaaaata atcgcatatg tatataaccg caatagatta aaacagaaaa
    12961 tgccctcatc gttattatcg taaaggcggg aatccgaaag ctcgacgtta aagccttatc
    13021 aggaatgact ggaactcaaa aaacggtttc ccgctttcgc ggaaatggcg ggattttcgg
    13081 tttttatttg caaaaatgcc ggagaagaat gcagtttaca ttttgaatgg tattgttatt
    13141 aattgtaaat aaatattgga aggtaaatga tatgaaaaat gaaattcaaa aaatcatgga
    13201 caaatataat ccatggcatg aagatgattt tgagtcgtat gaagacattg ccaaagatgt
    13261 atcgctgatg acagataaaa cgttcattga acattatttg ttagaagttt attcagaaga
    13321 aaacggacat tttgaccaag agaatgtcca tgcaatgata gaagaaatta aaaatgcaat
    13381 ttagtgaat
//
LOCUS       DAXHNE010000041        13137 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.041, whole genome shotgun sequence.
ACCESSION   DAXHNE010000041 DAXHNE010000000
VERSION     DAXHNE010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13137
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.041"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            202..414
                     /locus_tag="ACFM4Z_000908"
     CDS             202..414
                     /locus_tag="ACFM4Z_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFC6020968.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            562..2145
                     /locus_tag="ACFM4Z_000909"
     CDS             562..2145
                     /locus_tag="ACFM4Z_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HFC6020969.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESGAFSQTAI
                     NNLEARARLMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTIDESFGKFDYIIVHGIWSWVPDAVKDKIFSICWNNLT
                     KHGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLDGGGKLPAIQKILNHNFYYIAHEYMEAFNDPIYVNGFIEWANRHRLAYIGDTNLH
                     VSFVSWMAEHTRERILALAGDDYIAKEFYSDILSDRQFRRSLLCREEVGDTVRRDESV
                     AVEVIESLNFRPARGETINFDENDILLSGIRDVMKTGEAFKTEDVAENLARRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTNYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            2227..3324
                     /locus_tag="ACFM4Z_000910"
     CDS             2227..3324
                     /locus_tag="ACFM4Z_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFC6020970.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFSWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            3438..4553
                     /locus_tag="ACFM4Z_000911"
     CDS             3438..4553
                     /locus_tag="ACFM4Z_000911"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFC6020971.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNALP
                     VCGQSLLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            4678..5133
                     /locus_tag="ACFM4Z_000912"
     CDS             4678..5133
                     /locus_tag="ACFM4Z_000912"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFC6020972.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            5204..5680
                     /locus_tag="ACFM4Z_000913"
     CDS             5204..5680
                     /locus_tag="ACFM4Z_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFC6020973.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQQ"
     gene            5725..6018
                     /locus_tag="ACFM4Z_000914"
     CDS             5725..6018
                     /locus_tag="ACFM4Z_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFC6020974.1"
                     /translation="MKPSLLLQSRKKEILAVFGKYPLICNPRVFGSVSRGDDTENSDI
                     DLLVDAKTGTTLLDLGGLQEELQKLLGIKVDLLTPDDISAHFRDKVLTEAVAL"
     gene            6021..6287
                     /locus_tag="ACFM4Z_000915"
     CDS             6021..6287
                     /locus_tag="ACFM4Z_000915"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HFC6020975.1"
                     /translation="MQKELSVYLKQILQAAQYIRLYTDKMDYGQFSADTKTVQAVVFN
                     LFLIGENATHILKSYPEFAEETKYLNWIGMRGYCAIGLPMATLK"
     gene            6284..6409
                     /locus_tag="ACFM4Z_000916"
     CDS             6284..6409
                     /locus_tag="ACFM4Z_000916"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020976.1"
                     /translation="MNLSVVWETVLNVIPAMSADISNLLEQLSIDEEHDNSCHHF"
     gene            6470..7387
                     /locus_tag="ACFM4Z_000917"
     CDS             6470..7387
                     /locus_tag="ACFM4Z_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC6020977.1"
                     /translation="MKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEFGEI
                     GIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAHAAS
                     VIDELKTKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLVASKTDLCT
                     PEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIQWLDIPVIEKSRYRLKALPDNTMGFQS
                     QGFTFPAGRDFDGEKLTNFFNDLPNMTEGLVRAKGVFQVLGTWVWLNWVDGQWGANQV
                     SWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            7452..8009
                     /locus_tag="ACFM4Z_000918"
     CDS             7452..8009
                     /locus_tag="ACFM4Z_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6020978.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDDTVFHRVIDGFVIQGGGLTEDLAQKASDKAVANESSNGLKN
                     TAGTIAMARTTAPDSATSQFFINLADNASLDYKNGQYGYTVFGRVESGMNTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVGQ"
     gene            8125..10143
                     /locus_tag="ACFM4Z_000919"
                     /pseudo
     CDS             8125..10143
                     /locus_tag="ACFM4Z_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580243.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            10156..13095
                     /locus_tag="ACFM4Z_000920"
     CDS             10156..13095
                     /locus_tag="ACFM4Z_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFC6020979.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFM
                     IVDEPHKFPTRDSAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFLDGIIDDNENFAE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFADLFTAYLHENAAKFPQAGIRTAVNEAYINNGLMLSRRIDSFEDEDFIR
                     FNTMTYREFLEKLAQTAKIRMQTLHQAFYCIRNELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTNKDGKPRAVKKADLGRFEDTEHRPAAGYLFGEIF
                     YDSDIEHENVANNQIEGVIVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGR"
BASE COUNT         3681 a         3126 c         3303 g         3027 t
ORIGIN      
        1 atgcgccgat tgccaaggca atgatgctca accagggttt ttgcgaagcg ttcattgttg
       61 ttgtcctgat gggaattgcc gccattatgc gcaagcgtcc gtattttttc aaaccgccgc
      121 accgtttccg cgtttgccca agttcacgcc ccgacttaca ataacgcccg tcagaatgca
      181 gtaccaacga aaggaaaaat catggaaacc cttatcctcg acatcggcgg catgagctgc
      241 ggcggctgcg tcaaaagcgt tacccggata ttggaaggcg taaaaggcgt ggcaagcgtc
      301 gaagtcagcc ttgaaaacaa aagtgcgacc gtcggatacg accccgcgca aaccgatgca
      361 ggggcgttga ttgaagccgt tgaagacggc ggctatgatg ccgcgttgaa ataaagcggc
      421 aaaaatgccg tctgaagcct tcgcgcctcc cagatgccgt ctgaagtacg ctccgcgcct
      481 tcagatggca tttgatttag ttgaacgggt aggatgaatt ggttaaactt gcgggcaata
      541 gtgccttcag acaaacaaaa tatgccgaac aagaagaaaa aagccgcctt tgcgcgtgct
      601 gcaacggaga acggtgtgcc ggacgtgtcc aacatcaaaa attcatatga tgatttgatg
      661 tacgaatccg gcgcattctc tcaaaccgcc atcaataatt tggaggcgcg cgcccgcctg
      721 atgggtttgc agcccgcgcc cgccgccaat gccaaggtct tggagctggg ctgttcgatg
      781 ggcggaaaca tcatcacgca ggcactttat tacccggatg cggaatttgt cggtatcgac
      841 ctgtccggca ggcaggttgc acagggcaac gccatcattg aaaaaatggg cttgaaaaat
      901 gtgcgtctgg aagaaaaaga tattttgacc atcgatgagt cattcgggaa gtttgactat
      961 atcatcgtcc acggcatttg gtcgtgggtg cctgacgcag ttaaagacaa aattttttcg
     1021 atttgctgga acaacctgac caaacacggc attgcctata tttcatacaa tgtttacccc
     1081 ggctggaaac ggcaggagca gttgcgcgaa attatgtact ttgccggcag ggatgtgctt
     1141 gaagaaccct tggaagcgcg gacgcggaaa ggcttggacg cgctcaaggc gctggcggaa
     1201 attttggaaa acgacaaggg cttggacggc ggcggcaaac ttccggcgat tcaaaaaata
     1261 ttgaatcata atttttatta catcgcacac gaatatatgg aggcgtttaa cgacccgatt
     1321 tacgtcaacg gcttcatcga atgggctaac cgccacaggc tcgcatatat cggggatacc
     1381 aatttgcacg tgtcttttgt ttcttggatg gcggagcata cgcgggagcg gattctggca
     1441 ttggcagggg acgattatat tgccaaagaa ttttacagtg atattttatc cgaccgccaa
     1501 ttccgccgtt cgcttttatg ccgtgaggaa gtcggggata ctgtcaggcg tgatgagtcg
     1561 gttgccgtcg aagtgataga aagtttgaat ttccgcccgg caagagggga aacaatcaac
     1621 tttgatgaaa acgatatcct gctttcgggc atacgcgatg tgatgaaaac cggagaggcg
     1681 tttaaaacgg aagatgttgc ggaaaatctc gcccgccgat ttcccggttt ggaatttgac
     1741 cgcatgaaaa tcaattccca gcttttattg caaaccattc tcgggcgttt ttctgtttca
     1801 tcagacaatg cgggcaaacc attttttgaa gaccataaaa cctatgtgcc ggcgcgcttt
     1861 acaaactatg ccgccgcctt tgtggaacac ggcgcggaag cgtttgtccg gcctgccaac
     1921 cgttacaacg aaagcacccc ctcattcgga tacgggcatt tgtacattat gcgccaattg
     1981 tcgcggccga cgagcaaaca ggcattgatt gaaacggttg ccgaaaactt gaacatcgtc
     2041 agcaccacgc ccgacggttt gacattccat ccgcctgccg aagtgtatgt ggaagaaata
     2101 ttggcagact tggcagacag gcattttctc gtttcggcgg attgacgggc aagtcccggc
     2161 ggcaatccgt ccgatatatt ttcagacagg aaagacacac actgcgaaaa gcggtgcacg
     2221 acacatatga accagacatt taccttgccc gatacgcgcc cgtatcccca aaatccgatt
     2281 aaaaaccacc tgctgctcaa tgcctaccag ttggcgcata attcttccca ggcttcgcgc
     2341 aaactctcgt ccggccaact tcaaaccgaa atcaggggaa tgcttgagca aaaccactat
     2401 atcaaccttt ccctcgcgct gacgatgtcg cccgatgccg gaacttatgc cgcgctgctt
     2461 tccagtgtga acgcggtact cgattgcgag aaagaaggcg aagtgcagtg gttcgccctg
     2521 ccggtcgtgc tggtgtccgg ctgcaaaaaa gaacgtgcaa tcgagatgaa gctgccgacg
     2581 gaggcattgt ttgcctgcct gcaaaactat ccgcacctgc gcgcgttgac gcaagagacg
     2641 caatggctgc cttatcttgt gcattcttcc gatttgagcg cggtcgcgcc ggatgagtgg
     2701 tggcgtgcca aacaaaatac cgaagcggcg gcgcaacact tgcgccgttt cgccccgcgc
     2761 cctttgctgt tgcccgaagg gcagtccgtc cacgtcgttt acgcgctggg tttcggcagc
     2821 ggcaaagttc agacggcatt gggtcagaac cttcttcagg caggtctgcc cctgatgcag
     2881 gtatggcagg aaaatcttgc atcggaaggc gttacgctgt ttgccaatcc gctttccccg
     2941 gattctccgg tacgcgcgct ttcagacggc agccacacgc gccaacgtat ggcgatggat
     3001 gtgtttgcgg caaacgcgat acgcgccgtc cgtatgcaga gtccgcgcgt cggcgtggtc
     3061 gctgcggcaa aggcgggcgg acagatttta ttcgggttta atgcgaccga cggcgcgttt
     3121 gaagtcgtgc cgcaggtgtt ttcgtggcag ctttccttta ccgacaacat cgccgtcatc
     3181 cagcaaaatt tcctcgacct gatggcggag tgccgcgtgg aacacgttta cctgttgcac
     3241 aatcccttgg gcgaacagga aagcatcccg agctatgcgg aagcattgaa acgggaaggg
     3301 cacaatccgt ttttcagcgc atagtgattt gtagtagtga taggctttct catttatata
     3361 ataaatcaaa ataaagagca caacactttt tcattctgtg ttgtgccttg agtgaaacga
     3421 aaggatgaat tataaatatg aagattgtaa ttgcaccgga ttcgtttaag gaaagcttga
     3481 cagctcaaca ggtagctgaa gcaataaaaa gaggcttcca acaatcgata gcagatgtgg
     3541 aatgtctcct ctgtcctgtc ggtgatgggg gagaaggcac tgtagatgct atccgacatt
     3601 ctcttgacct agaagaaaaa tgtctccaag tgacaggacc ttttggacaa aaagaagtca
     3661 tgcgctattt tcaaaaagaa caactagccc tattcgaagt tgctgatttg gttggtcttg
     3721 gaaaaatccc gctagagaaa cgaaatccat tacaaatcca aactcgtggt attggagaat
     3781 tgattcgcca cctcattagt caagaaatta aagaaatcta tattggcgtt ggcggtacgg
     3841 ccagtaatga tggaggtatt gggattgctg ctggtttagg ttatcaattt tatgatgagg
     3901 atggaaatgc cttacccgtt tgcggtcaat ccttactaaa cctagcttct gtttcaacag
     3961 aaaatcgcta tgaaattcct gaagatgttc acattcgtat tttagcagat gttgtgagtc
     4021 ccttatgtgg acaccaaggt gcgacctata cgtttggcaa gcaaaaaggg ttggattcta
     4081 ctatgtttga ggccgtagat caggcaatac aagattttta tgaaaaagtc tcccctgcaa
     4141 cattaaaact taaaggagca ggagctggtg gaggcatcgc tggtggtttg tgcgcctttg
     4201 ctcaggcaag tatcgtatct ggaattgaca cctgcctgga cttgattgac tttgataaga
     4261 aagtgtcaga tgttgacttg gttatcgttg gtgaaggaag actagatcgt caaagtttag
     4321 cagggaaagc gcctattggt gtagcaaaaa gaacccctgt cggagttcct gttgtcgcta
     4381 tttgtggcag tctcgttgaa gatttgcctt ccctgccatt tgaaaatatc caagctgcct
     4441 tttctatttt ggagaaaagt gaacctttag aagatagttt gaaaaatgcc agcctctatt
     4501 tggagcatac ggcttctaat attggacact tattaaatat gcctaagatt tagccaaacc
     4561 atttcttcca gatggatgtt ttggctggtt ctgccttatg cggttacgat taaccgaccg
     4621 atgccgtctg aaagggtttc cgcttcagac ggcatcgcgc cggaacaggg aggggatatg
     4681 aaaaaaccga aaatcctttt tgtctgcctc ggcaacatct gccgttcgcc gatggcggaa
     4741 tacattttgc gccgccgcgc cgccgaagcg ggcattcccc ttgaagcgga cagcgcgggg
     4801 acatcgggct ggcacgacgg cgaagatatg caccgcgaga cggcaaagat attgaaaaaa
     4861 cacggtatcg atgcttcagg ctttaccagc cgcaaaatcc gccaaagcga tgcggcggcg
     4921 tttgactgca tcatcgcgat ggacggcaag aatttgtccg aattggaaag aaccttcggc
     4981 aggcggccgg aaaaaatatt caagctgacc gacctgatac ccgaaagcgg ttacgaccat
     5041 gtccccgatc cgtggtatac gggcgatttt gaagaaacct tcaggcttgc ggatgcgggc
     5101 tgtcgggcat tgttggaaaa gatttccaaa taaagcaatt gaatacaaat ataaaaccgc
     5161 ctcctgccgt atcggttgtt cagacggcat aaacagagaa tttatgaaaa caaatttcaa
     5221 acagaaaatt atcgaacagg cacgcagcga aggcttgcag gtaaccgctt tgcgcgagca
     5281 ggtattagat attgtcttgc agcaaagcgg cgtgattaaa gcctacaacg tcttgtcgca
     5341 gatgcagcag caaagcgagg gcgtgcttgc accgcctacc gcctaccgcg cccttgattt
     5401 ttgggcggag cagggcgttt tgcacaaagt ggcggcggtc aacggctata ttttgtgcag
     5461 ccacgcgcag cacgagtgcg acgaccattg ccacgaccac gaagaagccg aagcgcacca
     5521 cagcgcgttt attttggtct gcaccgaatg tggcacggcg gacgagcaaa ccctgagcca
     5581 cgagtgggcg gcactgcgcg caggcgttgc cgaaagcggc tttgcgctga aagaagaaca
     5641 cgttgtttta accgggattt gtaaaaaatg tcagcagtaa tcggtggttt gcattgataa
     5701 taagcctttc gaaaggagca gataatgaaa ccgtctcttt tgttgcaaag cagaaagaaa
     5761 gaaatattgg cagttttcgg gaaatatcca ttgatttgca atccaagggt attcggttcg
     5821 gtttctcgcg gagatgatac ggaaaacagc gacattgatt tgttggtgga tgcaaaaaca
     5881 gggacaacat tattggattt gggcgggctg caagaggaat tgcagaaact cttgggcata
     5941 aaggtcgatt tgctgacacc cgatgacatc tcggcccact ttagagataa ggtgttgact
     6001 gaggctgtcg cattatgaaa atgcagaaag aattgtcggt ctatttgaaa cagatattgc
     6061 aggcagcgca atatatccgg ctatataccg acaaaatgga ttatgggcaa ttttctgccg
     6121 acacaaagac tgtgcaggca gtcgttttta atttgttcct tatcggtgaa aacgcaaccc
     6181 atatccttaa atcgtatccg gaatttgccg aggaaaccaa atatttgaat tggataggga
     6241 tgagggggta ttgcgcaata ggattgccca tggctacttt gaaatgaacc tgtctgtcgt
     6301 ttgggagacg gttttgaacg tcataccggc gatgtcagcc gatatatcga atttgctgga
     6361 acaattgtca attgatgaag aacatgataa cagctgccat cacttttaac attatttaat
     6421 catttaaaac accctatctt caaattttaa agtgggacat cacatagaaa tgaaaaaaac
     6481 caaagtccac ctgatttcag gttttctggg aacaggcaaa accaccgcgc tcaaaagcct
     6541 gatggcgcaa aaagacccga acgaaaaatg ggtcatcatc gtcaacgagt tcggcgaaat
     6601 cggcattgac ggcgcggtat tgagcgacaa cggcatccct gtggcagaaa tcgccggcgg
     6661 ctgtttgtgt tgcaccgccg gcccgcaaat gggcgtaacc gtgcagaaaa tgctgcgcga
     6721 cgccaagccc gaccgcctga tgattgaagc aagcggactg gcgcacgccg ccagtgtcat
     6781 tgatgaactg aaaaccaaac cgctggacag ccttttggaa atcggcgcag tctttaccgt
     6841 cgtcgatccg cgccagttca tcaaccccga ttacgcgcag caggcattgt ataaagacca
     6901 aatcggcatc tgcgacgtat tggttgccag caaaaccgat ttatgcaccc ccgaacagct
     6961 tgccgaattt cacgacaaag ccgcaaaact gttcccgccc aaggctaaag tggtcgaagt
     7021 tcaaaacgca caactcgata tccaatggct tgacatcccc gtcatcgaaa aatcacgcta
     7081 ccgcctcaaa gccctgccgg acaacacgat gggcttccag tcgcaaggtt tcacattccc
     7141 cgccggacgc gatttcgacg gtgaaaaatt gaccaacttc ttcaatgatt tgcccaatat
     7201 gaccgaagga ctcgtccgtg ccaaaggcgt gttccaagta ttgggaacgt gggtgtggct
     7261 caactgggtg gacgggcaat ggggtgcgaa ccaagtgtct tggcggcgcg actcgcgttt
     7321 tgaattgatt gccaaatcgt ttgatgcgga tttaatcgaa caaaaactca aagacgcatt
     7381 ggaatagcgt taacgagcct tcagacggca taggaaatga aaatgccgtc tgaaggtctg
     7441 aggaggacgg gatgaaaccc aaattcaaaa ccgttttaac cgcgctgctt ttggcggttt
     7501 ccctgccgtc tatggcggca acccatgttt tgatggaaac cgatatgggc aatatccgtt
     7561 tggttttgga cgaatccaaa gcccccaaaa ccgttgctaa tttcgtgcgc tatgcccgaa
     7621 aaggctttta cgacgacacc gtttttcacc gcgttatcga cggttttgtt atccagggcg
     7681 gtggattgac cgaggacttg gcacaaaagg caagcgataa ggccgttgcc aacgaatcca
     7741 gcaacggctt gaaaaacacc gccggcacca tcgccatggc gcggacgaca gcccccgatt
     7801 ccgccaccag ccaattcttt atcaatctgg cggacaacgc ttcgctcgac tacaaaaacg
     7861 gacaatacgg ctataccgtt ttcggcaggg tcgaaagcgg catgaacacc gtttccaaaa
     7921 tcgcccgcgt caaaaccgcc acgcgcggct tttatcaaaa cgtacccgta cagcccgtca
     7981 aaatccgtcg cgttgttgtc gggcagtaac acgcagacag acgttcagac ggcgtcgccc
     8041 gtttcccaaa aaacgccgtt taaagtaaaa aaatatttta aaacagacag ttgatattga
     8101 caaattcaaa ccgaggattt taaaatgctg ccaacccaac ccaacccaag ccctgatcta
     8161 agcgacacag ccggggcgaa cactgaggca atctacactt cagacggcat taccgccaac
     8221 tctacccaac tcgaacagct caaaaaactg tttcccgcct gttttgacgc agacggaaat
     8281 ttccttatcg acagattaca agccgaaatc gcgccgcaga ccgacatcgg acgcgaattt
     8341 tacgaaatga actggctggg caaatcatat gctcgcctgc ttcgcaacct gccgcccgaa
     8401 accctgataa gtgaagacaa gacgcacaac gccaagcccg aaaatgcagg cagccaaaac
     8461 ctgctgattc gtggcgacaa tctagaagtg ttgaaacact taaaaaacgc ctacacaaac
     8521 agcgtgaaga tgatttacat cgacccgccc taaaacaccg gatcagacgg ctttgtctat
     8581 caggacgacc gcaaattcac acccgctgaa cttgcccgcc tagccaatat tgatgaagac
     8641 gaagccgcgc ggattttaga tttcaccgac aaaggctcaa actcgcacag cgcatggctc
     8701 acctttatgt atccgcgcct gtatgtcgcc cgcgaactgt taaaggacga cggtgtgatt
     8761 tttatctcga ttgacgataa cgaagcggcg cagttgaaat tgttgtgtga tgaagtgttt
     8821 ggggaaggga attttgttgc acaattgcct tggcgaaaaa gaacagctaa atcagatgtg
     8881 ccttttggta tttcgcagga ttatgaatgg atatttgtat tcgcaaaatc ttgccaattt
     8941 attgcagcaa ctaaaggcaa ggaacgacgc tattatgaga ctgatgattt ccccgatcgt
     9001 ccttggcgta cccacgactt aacgaaacaa acaactgcgg cggaaaggcc aaatagtttt
     9061 ttcacaatgg ttgatcccaa gacaggaaaa aaatatccag caaatccaaa tgcaacttgg
     9121 cgtgtaacca aagatacatt tcaagattat tataataaag gaaaaatcgt tttccctgat
     9181 gattatgatt tcttaaatat cagcaatcca gttatgcgtt actttaaaga tgacgacatg
     9241 aaaaaagctg gcgaggattt tggcaaagta gctgtaagca gtagattacc tgaaaatgtt
     9301 ggtacattag ccgatgcagt agccgaatat ttggctattt ttagtaggac gctacctgaa
     9361 aatatcggaa tgactaaaga aggcacaaaa gaaatcacag atttatttgg tagcaaaata
     9421 tttaccttcc ccaagcctag tcaattgatt aaatttttag tttcaataag ttcaaagagt
     9481 aatgacctaa tcctagactt cttcgcaggc agcggcacaa ccgcccacgc cgtgatgcag
     9541 cttaacgccg aaggacaaaa cggtaaccgc cgctatatct gtgtacagct tcccgaaaaa
     9601 accgctgaaa aatccgaagc ccgtaaagca ggctacccga ccatcttcga catcaccaaa
     9661 gcccgcatag aaaaagccgc cgccaaaatc cgcgtcgaac atcccgatta cacgggcgat
     9721 tcgggcttca aaatctttca aacggcagac aatttcaggc agcatccgga caaggatttt
     9781 tcgcccgaac aaccggattt gccgcttaac gatgaattaa gcgaagaaca gctgcaaacg
     9841 cttctgacca cctggacgct gtatgacggg gcggcactga ccacgccggt tgagcctgtg
     9901 cggttagggg cttacacggc gtatctgtgc gaaaaacggc tgtatctgat gaatgccggt
     9961 tttacttccg ccgatttgtt ggcgtttatc cgcaagctgg acgacgatgc ggatttcaat
    10021 cccaaccgcg tgattgtatt cggcagcaat atggcaagcg ccatgcagca cgaacttgac
    10081 caggcggttc gcggttatgc caataaaaaa gagattgagt tgaatgtggt tatcagggtt
    10141 tgacggaggc aattcatgag cggttttaat tacgagaaaa accagccgca ccaaatgcgg
    10201 gcggtttcgg cggttttggg cgtgtttgac ggggcaacgc ccaaatatcg gacggcagac
    10261 gaaaatcccg aacttttgtt tgctgcaaaa caatacgcaa acaatatcct gaaagtgcaa
    10321 agccaaaacg gtatagacgg ccgattcccc gaccgttcgg acgaccaaaa tatccttgat
    10381 atttccatgg aaacgggcac gggcaaaacc tatacctaca cacaaaccat gttcgagctg
    10441 caccgttggc tgggcgtgtt caaatttatc gtggtcgtgc cgactttgtc cattaaggcg
    10501 ggaacacagc agtttttgca aagcaaggct ttggcagagc attttgaaca ggatttcggc
    10561 ggcgattatg aaggcgtacg cctgaaaacc tatgtggtgg aaagcgcgaa aaagaataag
    10621 ggcaaaaagt ccaatgcgcc cataacgatt gagcaatttg tcaaagcgga aaacaaaaag
    10681 gaaattcatg tgctgctgat taacgcgggc atggttaatt cgtcgtccat gaacgatacg
    10741 ggcgacaagg cattgaagga tttgtttgac aatcccgttg atgcattggc tgccgtgcgc
    10801 ccgtttatga ttgtggacga accgcataaa ttcccgaccc gagatagcgc gaaaacgtgg
    10861 ggcaatatca aacgcttaaa accgcaatat attttgcgct acggtgcaac atttaacgat
    10921 gaatattaca acttgcttta ccgtttgacg gcagtagacg cgtttaacga cgggctggtc
    10981 aaaggcgtgc gcgtgtttca ggaagaaatg cagggcggca tggatgcggc ggtaaaactg
    11041 gtgtcgtcgg acggcaaaga agcgaaattt gaattaaacg aaaaggacaa aaagcagacg
    11101 ttcaaactgg caaaaggcga agatttggcg caaatccatc cggctatttc ggatttgaaa
    11161 atcgacaaaa tgaataaaac cgtggtggtg ttaagcaacg gcttggagtt gaaaacgggt
    11221 gccgtcatca acccttattc ctattcgcaa acggtgcagg atgcgatgat gcagcgggcg
    11281 gttgccgaac atttcaagct ggaacgcgcg cttttggcag aacgcgcgcc acagcccaaa
    11341 atcaagccgc tgacgctgtt ttttattgac gatatcgcgg gctaccgcag cggcaacgag
    11401 ctttcaggca gcctgaaaga taaatttgaa agctggattc gcgcggaagc ggcacgccgt
    11461 ctgaaaacgg aaagcgaccc gttttaccgc gattacctgc aaaagacgtt ggacgatgta
    11521 tccgcctgcc acggcggcta tttttccaaa gacaatacag acagcgacga tagaatcgag
    11581 caggaaatca atgaaatcct gcacgataag gaaaaactgc tgtctttgga caacccgcgc
    11641 cgctttattt tttccaaatg gacgctgcgc gaaggctggg acaatcccaa cgttttccag
    11701 atttgcaaac tgcgttccag cggcagcacg acttccaagc tgcaagaagt cggacgcggc
    11761 ctgcgcctgc cggtaaacga gctgatggcg cgggtgcgcg atgtaccgta caaactgaat
    11821 tattttgtcg atagcagcga aaaagacttt gtgaagcagc ttgtcggcga aatcaacgac
    11881 aattcttttc aggaagaaat ctccaaaaag tttaccgaag agctgaaaca aaaaatattg
    11941 caaaaatacc ccgatatcaa accgctggta ttggtaaacc aactgttttt agatggcatc
    12001 attgacgaca atgaaaactt tgccgaagac ggctatgaca aattaaaagc cgcctatccc
    12061 gaagccttcc ccaaaggttt ggacaaaggc aaagtcagca acgccaaaga cgaaggcaaa
    12121 gacaccatca tcatgcgcga aggcaaatat gaagaactca aagccttgtg ggagctgatt
    12181 caccataaag ccgttttgca gtataaaatc aaggatgaag ccgaatttgc cgatttattt
    12241 accgcctatt tgcatgaaaa cgctgccaaa ttcccacagg caggcatacg cacggcggta
    12301 aacgaagctt atatcaacaa cgggcttatg ctttcccgcc gcatagacag ttttgaagat
    12361 gaagatttta tccgtttcaa cacaatgact taccgagagt ttctggaaaa actggcacaa
    12421 acggcaaaaa tccgtatgca gactttgcat caggcgtttt actgcatccg caacgaactg
    12481 aacattggcg attttttgaa tatgcaaacc atcgcccaaa tcaaaaacgg cttcaaccgg
    12541 tttttgcttc atcattcctt ccataaattc gaactggatt accggcttgt cggcagcaaa
    12601 atccatccga ccaaatttac caataaagac ggcaaaccgc gcgcggtgaa aaaagcagat
    12661 ttgggcagat ttgaagatac ggagcaccgg cctgccgccg gctatctctt cggcgagatt
    12721 ttctacgatt cggatataga acatgaaaat gtcgccaaca accaaattga aggcgtaatc
    12781 gtatttacca aaataccgag aaactccatc aaaatccctg ttgccggcgg cggcacgtat
    12841 tcgcccgatt ttgcctatat cgtgaaaacc aaaagcggcg agatcctgaa ctttgtgatt
    12901 gaagccaaag ggactgacgg ggcggaagat ttgcgaaaaa gcgaagagcg gaaaatcaaa
    12961 catgccgaaa agctgtttgc cgagatttcc aaagaaatca aggtggtgtt caaaacgcag
    13021 tttgacggcg agaggatagc cgaactgatc gggcaaaata tgcccgcagg cgggcattct
    13081 gaaaacgggc gttaaagaac gaatgttcgg ggcgcgtgcc gtctgcttcg ggatttt
//
LOCUS       DAXHNE010000042        12894 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.042, whole genome shotgun sequence.
ACCESSION   DAXHNE010000042 DAXHNE010000000
VERSION     DAXHNE010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12894
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.042"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..325
                     /locus_tag="ACFM4Z_000921"
     CDS             <1..325
                     /locus_tag="ACFM4Z_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HFC6020980.1"
                     /translation="TEITLETPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNN
                     ADTGFPSESVGSDAPSEAKYDLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALA
                     QELGI"
     gene            complement(498..914)
                     /gene="gloA"
                     /locus_tag="ACFM4Z_000922"
     CDS             complement(498..914)
                     /gene="gloA"
                     /locus_tag="ACFM4Z_000922"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFC6020981.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(938..2107)
                     /gene="lapB"
                     /locus_tag="ACFM4Z_000923"
     CDS             complement(938..2107)
                     /gene="lapB"
                     /locus_tag="ACFM4Z_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFC6020982.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVVDGRPQSYDLNLTLGKLYRQRGENDKAINMHQTLLD
                     SPDTTGAKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(2118..2432)
                     /locus_tag="ACFM4Z_000924"
     CDS             complement(2118..2432)
                     /locus_tag="ACFM4Z_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly LapA
                     domain-containing protein"
                     /protein_id="HFC6020983.1"
                     /translation="MKLIYTVVKIIILLFFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIIFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESTK
                     QP"
     gene            2742..3740
                     /gene="ilvE"
                     /locus_tag="ACFM4Z_000925"
     CDS             2742..3740
                     /gene="ilvE"
                     /locus_tag="ACFM4Z_000925"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFC6020984.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVAELKAAVENGAEA
                     ILTGTAAVISPVTSFVIDGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(3984..4769)
                     /gene="fabI"
                     /locus_tag="ACFM4Z_000926"
     CDS             complement(3984..4769)
                     /gene="fabI"
                     /locus_tag="ACFM4Z_000926"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFC6020985.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGADLAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(4924..5745)
                     /gene="dapD"
                     /locus_tag="ACFM4Z_000927"
     CDS             complement(4924..5745)
                     /gene="dapD"
                     /locus_tag="ACFM4Z_000927"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFC6020986.1"
                     /translation="MSLQNIIETAFENRADITPTTVAPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQASPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(5755..5946)
                     /locus_tag="ACFM4Z_000928"
     CDS             complement(5755..5946)
                     /locus_tag="ACFM4Z_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020987.1"
                     /translation="MPSERRRRGIKAMPAARPIPKRQCSATARVLLTNMRLRGTIAHS
                     FQMNILPENTGKRPTKHLT"
     gene            complement(5916..7559)
                     /gene="pgi"
                     /locus_tag="ACFM4Z_000929"
     CDS             complement(5916..7559)
                     /gene="pgi"
                     /locus_tag="ACFM4Z_000929"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC6020988.1"
                     /translation="MDAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCNLADAADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     GAVYADGRDVLPEIRRELNRALKFAHSLDDGSYQGTTGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDEVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETARHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGVGAA"
     gene            8069..9301
                     /gene="pilG"
                     /locus_tag="ACFM4Z_000930"
     CDS             8069..9301
                     /gene="pilG"
                     /locus_tag="ACFM4Z_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HFC6020989.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            9375..10235
                     /locus_tag="ACFM4Z_000931"
     CDS             9375..10235
                     /locus_tag="ACFM4Z_000931"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFC6020990.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKKHLNLPLTEEESRTFNLMKPDSCCPKCRVPIRARQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            10237..10869
                     /gene="coaE"
                     /locus_tag="ACFM4Z_000932"
     CDS             10237..10869
                     /gene="coaE"
                     /locus_tag="ACFM4Z_000932"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261706.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFC6020991.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHNG"
     gene            10862..11071
                     /locus_tag="ACFM4Z_000933"
     CDS             10862..11071
                     /locus_tag="ACFM4Z_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFC6020992.1"
                     /translation="MAESRQTRLQVKCPTCQTAVVWEPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDMAYR"
     gene            complement(11156..12832)
                     /gene="pilB"
                     /locus_tag="ACFM4Z_000934"
     CDS             complement(11156..12832)
                     /gene="pilB"
                     /locus_tag="ACFM4Z_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HFC6020993.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
BASE COUNT         2956 a         3575 c         3378 g         2985 t
ORIGIN      
        1 aacggaaatc actttggaaa cgcctgattc caacacctct gaggcagacg ctttgcccga
       61 cttcctgaaa gacggcgagg aggaaacggt agattggagc atctacctct cggaagaaaa
      121 tatcccaaat aatgcagata ccggtttccc ttcggaatct gtaggttctg acgcgccttc
      181 cgaagcgaaa tacgaccttg ccgaaatgta tctcgaaatc ggcgaccgcg atgccgctgc
      241 cgagacagtg cagaaattgc tggaagaagc ggaaggcgac gtactcaaac gtgcccaagc
      301 attggcgcag gaattgggta tttgattccc aactgccctt tcgcagatca aggatgccgt
      361 ttcagacggc atcttttttt gccttatcgg tgtaacggat aaagtttgaa ccggcacagg
      421 ctcaaacagc aggtcgacgg caacaaaatg ccgtctgaaa cccctaaagg cttcagacgg
      481 cattggcggc ggcggtatca ggcagtttga taggcaaccg aatcgtcacc gcttttcttt
      541 tgaatgaact cgattttgta tccgtcgggg tcttcgacga aggctatcac ggttgtgccg
      601 tgtttcatcg ggccggcttc gcggacgacg tttccgccct gccgcttcac acgttcacag
      661 gcttcgtagg catcgtccac ttcaaccgcg atgtgtccgt aggcgttgcc caagtcgtat
      721 cgttccgtat cccagttgtg cgtcagttcc aaaaccgtgc tgtcggtttc gtcgccgtaa
      781 ccgacgaagg caagggtaaa tctgccttcg ggataatctt ttcggcggag cagtttcata
      841 cccaaaacgt tttggtagaa atcgagggat ttttcgagat tgcccacgcg gagcatagta
      901 tggagtaagc gcatttttgt gttcctctcg gtagtggtta aacttcgatt ttgtttggcg
      961 taaacgtctg ccatttgttg caggcaggac aatgccagaa aaagacttgt gatttgaagt
     1021 ggcagtttcg gcaccggtac atcacgctgc gctgtagctg ccgtccgata accgaacgca
     1081 tcatatcggc atcggctttc caagccggat ccaaatcgct gagtttcaaa ccaagcaggc
     1141 ggtacacgcc gttgaggtcg ggcttgcggc ggacaagctc gacggcggtt tgcgcggctt
     1201 ctttctcgca cttaagcagc agggatttct cgtacacgac attgatcagg tcaagttcgg
     1261 gaaacgtctg catatatcct gtcagacggt tcaagccttc ttcaggtttt ccctgcgcgg
     1321 cataggcttc gtaaagcttc tcgccgacca tgctcaagta tgcatggttt tgctgctcga
     1381 tggcggcata ggcttcgacg gcggcaggga aattgccttg tcggtgttcg atgtcgccca
     1441 aaatcatgtt ggcacgggtg cattttttgt tggcttcgag tgccttgccg acattgaaac
     1501 gcgcgacatc gaaattggac ttgaacagcg cggcttgggc aagttcgcaa taaaactggg
     1561 cgatttcaaa ctgataggtc tgatcgtcat ggctgagcag ccgggcggtt tcaaccgctt
     1621 tttcccaatc cctgtcctgt tggtagatat tgagcaggtg ctgtctggct tcacgcgcca
     1681 ttttaccgtc ttgcagcccc aaaaaaatct gttcggcacg atcgaccaac cccgcacttt
     1741 ggtagttttg cgccaattca aacaggacgc gcgcgcgctt ggctccggtt gtatcgggag
     1801 agtcaagcaa tgtttggtgc atattgatgg ctttgtcgtt ttcgccacgc tggcggtaaa
     1861 gtttgccgag ggtgaggttc aaatcatacg attgcggccg gccgtcgacg acttccgcca
     1921 actcccttgc cgcgcgcccg ctgttgcggt cgaccaaagc gtccaagctt ttataaaatc
     1981 ccgaagggat gctttttgcc tgcttcaata cggttttcat atccacgcgg gcggcaaacc
     2041 agcccatcgc gaagaagacg ggcaaaagga taatcggcag caggataatc cacaattcgt
     2101 tgtccatatc ggctttctta aggctgtttg gtagattcgg gcgcattttg cgccggtggt
     2161 gcggtcagct ccttccccgt caaacgcgca tttttcttta cttcggcacg caacctgccg
     2221 ttctcgccac gtaacgacaa caaccgtccg aacaaggcaa acattccaaa aataataccg
     2281 actacaaatg cgccgaacaa tacgacaatc agcggcaaat cgaatttttg ccccggcagg
     2341 taggagaagg taacggcatc cgtattaatg acggcaagca gcaggaagaa cagcaggata
     2401 atgattttga cgacggtata gataagtttc atgtttgctc cgtaaataaa aacgccgccc
     2461 ggacagcttc gcactctgaa ccaagattgc gctagtttaa acgatttgca cggtttcgga
     2521 taggatgcgg cagcgtgttc ggacgacata cggagtatgc gattgccgac aattttacca
     2581 aatacaccgt tcctttccat ttgaaaaata acggattgga caccgcatcg acagaaaaac
     2641 ccgccgcgca cttgtcaaaa ccctgtttgc aggcgtatct ttacaatctt caaattcaaa
     2701 ccgttcattg aaacatatca gaataagaaa ggctttacat catgagcaga cccgtacccg
     2761 ccgtattcgg cagcgttttt cacagtcaaa tgcccgtcct cgcctaccgc gaaggcaaat
     2821 ggcagccgac cgaatggcaa tcttcccaag acctctccct cgcaccgggc gcgcacgccc
     2881 tgcactacgg cagcgaatgt ttcgagggac tgaaagcctt ccgtcaggca gacggtaaaa
     2941 tcgtactgtt ccgtccgact gccaatatcg cgcgtatgcg gcaaagtgcg gacattttgc
     3001 acctgccccg ccccgaaacc caagcctatc tcgatgcttt ggtcgaactg gtcaaacgtg
     3061 ccgccgacga aattcccgat gcgcctgccg ccctgtacct gcgcccgacc ttaatcggta
     3121 ccgatcccgt tatcggcaag gccggttctc cttccgaaac cgccctgctg tatattttgg
     3181 cttcccccgt cggcgactat ttcaaagtcg gctcgcccgt caaaatttta gtggaaaccg
     3241 aacacatccg ctgcgccccg catatgggcc gcgtcaaatg cggcggcaac tacgcttccg
     3301 ccatgcactg ggtgctgaag gcgaaagccg aatacggcgc aaaccaagtc ctcttctgcc
     3361 cgaacggcga cgtgcaggaa accggcgcgt ccaactttat cctgattaac ggcgatgaaa
     3421 tcattaccaa accgctgacc gacgagtttt tgcacggcgt tacccgcgat tccgtgctga
     3481 cggttgccaa agatttgggc tataccgtca gcgaacgcaa tttcacggtt gccgaactga
     3541 aagctgcggt ggaaaacggt gcggaggcga ttttgaccgg tacggccgcg gtcatttctc
     3601 cggtaacttc tttcgtgatt gacggcaaag aaatcgaagt gaaaagccaa gaacgcggct
     3661 acgccatccg taaggcgatt accgacatcc agtatggttt ggcggaagac aaatacggct
     3721 ggctggttga agtgtgctga tgctttaaat aaaaaatgcc gtctgaaacc cgtttggcgt
     3781 ttcagacggc attttcgcat ccgaaccgtt tccgctgcac ctgcagcaag tcggcacaaa
     3841 ggcaatcggt taaaacaagc gtccgcattt cccatcccgc ctgccgtaag tcgggcattt
     3901 ccctagaaat acgcttcaga cggcaaaacg ccgcccgaaa ccgatatgcg gcacggacgg
     3961 cgcacggatt tgaaaacggc ggattatccc tcggtgctca aggcattgat gctgtaaccg
     4021 ccgtcaacgt aagtgatttc gccggtaatg ccggacgaca ggtcggacag caggaaggcg
     4081 gcggtattgc cgacttcttc gatggtaacg ttgcggcgca gcggattgtg ggcggcgacg
     4141 tgtcccaaga gtttgccgaa atcggcgatg ccggaggcgg cgagtgtttt gatcggaccg
     4201 gcggaaatgc cgttgcagcg gatgccttct ttgcccaaac aggcggcggt aaagcgaatg
     4261 cccgcctcaa ggctggcttt tgccataccc atcacgttgt agttcggaat cgcgcgcacc
     4321 gcgcccaagt agctcagggc aacgatggcg gagtttctgc cgcgcatcat cggacgggcg
     4381 gcttttgcca atgcgggcag gctgtatgcg gaaatttcgt gtgcggtgtt gaacgcttcg
     4441 cggctgatgc tgtcgaggaa atcgccgctc aaggcttctt tcggtgcgaa gccgatggaa
     4501 tgcaccaaac cgtccaagcc gtcccaatgt ttgcccaagt cggcgaacac ttggttgatt
     4561 tcgtcgtcac tggcgacatc gcagcggaat acgagttcgg aatccaattc cgccgccatt
     4621 ttgcggacgc gctcttccag tttgtccaca acgtaggtaa acgccaaatc cgcgccttgt
     4681 tcgcggcagg ctttggcgat gccgtaagcg atggaacgct cggaaatcat gccggtaatc
     4741 agaatttttt tgccttgcag aaaacccatt ttgtatcctt caaatagtgt tcggttcgtt
     4801 ctaaaccgtt gattatagca aattgtccct gtttctgtgt tttcacgttg cagcgtgcaa
     4861 acggcaatgc cgtctgaagc ggatttcaga cggcatcgga cgtttcaaat acggtttaag
     4921 gcatcagatg ccgcgcaaca attcgttgac gctggttttc gcacgggttt gcgcgtccac
     4981 gcgtttgacg atgacggcgc agtaaaggct gtggctgccg tctttggaag gcatactgcc
     5041 ggatacgaca accgaacctg ccggtacgcg gccttggtag atttcgccgg ttgtacggtc
     5101 aaagattttg gtggattgac cgatgaacac gcccatagaa atcacgctgc cttcttcgac
     5161 aatcacgccc tcaacgattt cagaacgcgc accgatgaag cagttgtctt caataatggt
     5221 cgggctggcc tgcaggggtt cgagtacgcc gccgatgccg acaccgccgc tcaagtggac
     5281 gtttttaccg atttgcgcgc aagagccgac ggtcgcccaa gtatcgacca tcgcaccttc
     5341 gtcaacgtat gcaccgatgt tgacgtaaga cggcatcagc acgacatttt tcgccacaaa
     5401 gctgccgcgt cgggcaaccg cacccggaac tgcgcggaag cctgcgtttt tgaactcgtc
     5461 ttcagaccaa tcggcaaact tggtcggcac tttgtcgaag tatttgttca cgccgtcgtt
     5521 gaggacttcg ttgtcttgga tgcggaagga cagcaacacg gcttttttcg cccattcgtt
     5581 gactttccat tcgcccacgc ccaagcgttc ggcaacgcgc agtttgcctg aatcgagctg
     5641 gcggatggtt tccaacacgg cttctttaac ttctggggca acggtggtcg gggtgatgtc
     5701 cgcgcggttt tcaaaggcgg tttcgataat gttttgcaaa gacataatgt ttccttatgt
     5761 gagatgtttc gtgggacgct tgccggtatt ttcaggcagt atgttcattt gaaatgaatg
     5821 cgctattgtg ccacgcaaac gcatattcgt cagcaatacg cgggcggttg ccgaacattg
     5881 gcgtttcgga atcgggcggg cggcaggcat tgcctttatg ccgcgccgac gcctttcaga
     5941 cggcattcac ggtaaaacgc catcagccct tcggtcgagg catcgtgtac ggacgtgccg
     6001 ccttccagtt cgccgatgat ggttttcgcc aactgtttgc cgtattccac cccccactga
     6061 tcgaaggggt tgacgttcca tatcgcgcct tggacgaagg ttttgtgttc gtaagccgcc
     6121 atcagcatac ccagattgta gggcgtgagg cggtcgagca ggatgctgtt gctggggcgg
     6181 ttgccgggga attctttgtg cggcgcgagg cgttcgcgtt ccgcttcggg caaatctgcc
     6241 agttcggcgc gtgcttcgtc caaggttttg cccttcatca gggcttccgc ttgggcaaag
     6301 gcgttggcaa cggtaaaacg gctgcgtccg tcctccacgc cctgcgccgt catcgggacg
     6361 ataaagtcgc agggaatcag gcgcgtgcct tgatgaagca gttggaaata ggcgtgctgg
     6421 cagttgaccc cttcaccgcc gaacacgatg ccgcccgttt tgcacacggc gggactgccg
     6481 tctgaagcgc ggcttttgcc caaactctcc atatcgagct ggttcagcca cgccggcagc
     6541 aggcgcaggt tgtggctgta cggaacggcg gtctgcccgt ccgcgtgttg gaaattgtta
     6601 taccacacgg caatcagtgc catcagaacg ggaatgttgc ggcgcggcgg cgtgtggaaa
     6661 aaatggctgt ccatcgcgtg cgcccccgcc aacaactcgc ggaaacgcgc cccgccgacc
     6721 gcaaccatca cgggcaaacc gacgggcgac cagacggaat agcgtccgcc cacccagtcg
     6781 tacatcgcaa acacgcgttc cgccgcgata ccgaaagccg cagctgccgc tgtgtcggcc
     6841 gataccgcgc aaaaatggcg tgctgtttcg gattccgaga accccgcacc acgataccaa
     6901 gccttgaccg cctgtgcatt gagcagggtt tcaggcgttt tgaaggactt gctggcaacg
     6961 caaaacaccg tcgtttcggg gttcagacgg cataagacct catccaggca ggcaggatcg
     7021 gcgttggcgg caaaatggac ggcgatatgc cgtctgaacg gctcgagtgc ctgcacgcac
     7081 attgccggcc cgaggtcgga tccgcctatg ccgatgtgga caaaatccgt aatccgtttt
     7141 ccggttgtcc cctgatacga accgtcgtcc aaactgtgtg caaacttcaa cgcacgattt
     7201 aactcgcggc ggatttcggg caacacgtcc ctgccgtccg cataaacggc acccgcaccg
     7261 tcgggcaggc gcaaagccgt atgcagcgcg gcacgcccct cgctgccgtt gactttcgca
     7321 cccgtccgca aagcacgcat tttcccttcc aaatccgccg catcggcaag attgcagagc
     7381 agttgcagcg tatcttcgcc caaacggttt ttgctgtaat cgaacaacat cccgtccaaa
     7441 cgctcgtgca tacgctcaaa acggtcgggt tcgcaggcaa agcggtcgcg caaaaggaca
     7501 tgacgcgtat cctgataatg gcgttcgagc gcataccatg cacgggtaaa agcatccatc
     7561 tgttttcctt gatttttcag aaccggatta aaatgtagca gaatgtagtt taacaaacgg
     7621 cagcggcttt ggcgaatctc cggaacaccg cccgccgcgt ccaatagcac cttatttcca
     7681 tatggcgaaa atatagtgaa ttaacaaaaa tcaggacaag gcggcgagcc gcaaacagta
     7741 caaatagtac ggaaccgatt cacttggtgc ttcagcacct tagagaatcg ttctctcttg
     7801 agctaaggcg agccaacgct gtaccggttt ttgttaattc actataccct ccggccggca
     7861 tttgacccgc aacaatatcc tgcaagattt attgtgtacg cataaatgcc ggacagccgc
     7921 ctaaataagg tataatcgca tccgattctg tcccgtttgc cgcccgcagg cgggatgtcg
     7981 gcggttttca tcttaccggg aacacgccgg tactgttttt taacgatgtt tcttacattt
     8041 tattccaatt actttacggg gctagaatat ggctaaaaac ggaggatttt ctttgttcgc
     8101 aaagaaagaa aaacgcttta tctttgaagg caggcattcc gcctccgaca aactggtcaa
     8161 cggcgaagta tccgcgttta ccgaagaaga ggcgcgcaaa aaactggcaa aacgcggcat
     8221 ccgcccgttg cagattaccc gtgtgaaaac aagctccaag cgcaaaatca cacaagaaga
     8281 catcaccgtt ttcacccgcc agctgtccac gatgattaaa gcgggcctgc cgctgatgca
     8341 ggcatttgaa atcgtggcgc gcggacacgg caacccgtct atgacggaaa tgctgatgga
     8401 aatccgaggc gaagtggaac agggcagctc gttgagccgc gcattctcaa accacccgaa
     8461 atatttcgac cgcttctact gcaacctggt tgcggcgggc gaaacgggcg gcgtattgga
     8521 aagcctgctg gacaaattgg caatttacaa agaaaaaacc caggccatcc gcaaaaaggt
     8581 aaaaaccgcg ctgacctatc cggtatccgt catcgccgtc gccatcggtt tggtattcgt
     8641 gatgatgatt ttcgtactgc ccgcctttaa agaagtttac gccaatatgg gcgcggagct
     8701 tcccgcactg acccaaacag tgatggatat gtccgacttt ttcgtctcat acggctggat
     8761 ggtgctgatc gcactgggct ttgccatata cggcttcctt aaattgaagg cgcgttcgat
     8821 taaaatccaa cggcgtatgg atgccatact gctgcgtatg ccgattttcg gagacattgt
     8881 ccgcaaaggg acgattgccc gctggggcag gacgacagcg acgctgattg cggcaggcgt
     8941 gcctttggtc gatgtattgg actccactgc cggcgcggcg ggcaatttaa tctatgaaga
     9001 agccacccgg gaaatccgta cgcgcgtcat ccaaggtttg tctatgactt cggggatgcg
     9061 tgcgacggaa ctgttcccca atatgatgtt gcagatgtct tccatcggcg aggaatcggg
     9121 ttctttggac gatatgctca acaaagccgc cgaattttac gaagacgagg tggacaatgc
     9181 ggtcggcagg ctgtccgcta tgatggagcc gatcattatc gtgattttag gcttggtcat
     9241 cggcacgctt ctggtcgcca tgtatctgcc gctgtttaat ttgggcaacg tggtcgcctg
     9301 atttgccgca cagatccggc gcggattcgc tctgcgccgg tttgtttttg ttttgaatac
     9361 attgagaaca aaatatgtct gatttgtctg tattgtcgcc gtttgccgtg cctttggcag
     9421 cggtgttcgg gctgctggtc ggcagtttct taaacgtcgt catttaccgc gtgccggtca
     9481 tgatggaacg cggctggacg gtatttgcca aaaaacattt aaacctgccg ctgaccgaag
     9541 aggaaagccg taccttcaac ctgatgaaac cggattcctg ctgtcccaaa tgtcgcgtgc
     9601 cgatacgcgc gcggcagaac atcccgattg tcagctacct gctcctgcgc ggcaaatgcg
     9661 cctcctgcca aaccaaaatc agcatacgct atcccttaat cgaactgctg accggcgtat
     9721 tgttcgggct ggttgcctgg caatacggct ggtcttggat tacattgggc ggtttgatac
     9781 tgaccgcgtt tctgatttcc ctgaccttta tcgatgcgga cacccaatac ctgcccgact
     9841 cgatgacatt acccttgatt tggctggggc tgatatttaa tttggacggc ggcttcgtgc
     9901 ctttgcagtc tgccgtttta ggtgcggttg ccggctatgg ttcgatatgg cttttatgcg
     9961 cggtatataa actgattaaa ggcgaaatcg gtatgggcaa cggagatttc aaactgattg
    10021 ccgcattggg cgcgtggctc ggcatatccg cattgcccgt gctgattttt gtttcctcgc
    10081 tgatcggttt ggtcgcggca atcgttatgc gcgtcgccaa ggggcagcat tttgccttcg
    10141 gccccgcact gacagtttcg ggctggataa tttttacggc aaacgattcc gtatggcggg
    10201 cggtcaactg gtggctgacc catccggtgc tgtaagatga cggtatgggt cggactgacc
    10261 ggcggaatcg gcagcggcaa atcggcagcc gcgcaatgtt ttgccgattt gggcgtgccg
    10321 cgcatcgatg cggacgcggc ggcgcactcg ctgacggctt cagacggcat cgccctgccg
    10381 gaaatcaggc ggctgttcgg cgacaccgtt ttcgacacac agggtttgtt gcggcgcgac
    10441 atattgcgta aagaagtctt tgcctcccca tcgcgaaaag ccttgctcga atccgtgatg
    10501 ttgccgctga ttttctcaga aatcaaaaaa cagcaagaaa cctttaccga tgcggcttac
    10561 ggcattgtcg aaattccgct gctgacggaa aagcgtcaat ttatcagcct gatacggcgt
    10621 gtcctgacca taagcgcacc tttggaaaaa cgtatcggca gggtgatggc ccgcagcggg
    10681 ctgacgcgcg gcgaggtggc tgccgtcatc agccatcagg catccgaatc cgaacgcctg
    10741 ctgcttgcag acgatgtgct gctcaatgac ggcagcctca aaagcctgcg tgagaaaaca
    10801 atgcgcctgc acgcgtttta ttcagggatt ttcgcctcaa aaccaacaca aggaaaacac
    10861 aatggctgaa tcgcggcaaa cacgccttca agtcaaatgt ccgacctgtc aaacagcagt
    10921 agtatgggaa cccgaaaacg cattccgccc cttctgttcg caacgctgca aactgatcga
    10981 cttgggcgga tgggcagacg ggaaatatac ggtttccggc caaacggaaa gtttgccgga
    11041 aatatccgaa cccgacatgg cataccgctg accgccccgc cttcccggca aacaccctga
    11101 aagtcaaatg ccgtctgaaa caaacacgct tcagacggca ttttcattct caaacctaat
    11161 cgttggtatt tgccgttacc tcttccaatg aagtaatgcc ctgcataact ttcaaaatac
    11221 cggcccggcg caaatccacc ataccctcct tataggcaac gtccaaaata tccacttccg
    11281 taccgttgtt cataatcaca cgctgcattt cttcgctgat gggcataacc tcatacacgc
    11341 ccgcacgccc cttataaccc tgcccccggc aacggtcgca accgacggcg cggtaaagtt
    11401 tccaatcttt tgcaaggtcc tcatcggtga agccgacttc cttcaaagca gaggcagacg
    11461 ggcgttccac ttcctgtttg cagctcgaac acagcctgcg taaaagacgc tgcgccataa
    11521 tcaggctgac cgaactggca atattaaacg gcgcgacacc catattcagc atacgcgaca
    11581 acgtcgccgg cgcattattg gtgtgcaggg tggaaaacac catatgccct gtttgtgccg
    11641 ccttaatcgc aatatcggca gtttccaaat cacgaatctc accgaccata atgatgtccg
    11701 ggtcctgacg caggaaagac ttcaaagcag cggcaaaagt cagaccctgc ttatcattga
    11761 cgttaacctg attgatgccc ggcaggttaa tctcggcagg gtcttccgcc gttgcaatat
    11821 ttaccgactc cgtattcaaa atattcaaac aggtatagag cgacaccgtc ttacccgaac
    11881 ccgtcggacc ggttaccagc accatcccgt agggacggtg aatcgcttcc aacaacaatt
    11941 ttttctgaaa cggctcaaaa ccgagctggt caatgttcaa agacgcggca tcggaattca
    12001 aaatccgcat cacgaccttt tcgccaaaca gcgtcggcaa tgtgctgaca cggaaatcga
    12061 caggcttgcc gcccttttga aaggtcagct gcatcctgcc gtcctgcggt atccgttttt
    12121 cggaaatgtc caaacgcgac attaccttaa tccgggaagc aagctgcccc cttaccgcaa
    12181 tgggcggctg aaccacctcg cggagctgcc cgtccacacg gaaacggata cgggcattgt
    12241 gttcgtaaaa ctcgaaatgg atgtcggatg ccccgctgcg caaggcatcc gacaaagtct
    12301 tatggataaa cctcggaaca gggccgtctt ctgcctcctc gttgtcgata tacagggtgt
    12361 ggctttcctc ttcctcctgc ccctccccaa gctcctgaag cagcgatgtc gaacgcgaac
    12421 ccacccaatc gagcaaaccc gccaactggt catcctcgac aatgaccaat tcaacctcaa
    12481 tccctgcggc agaaacggtt ttctgaattt gcggcatctg tgtcggatcg gaaaccgcaa
    12541 aaaatacttt gtcgccccga cggaaaaccg gcacacagtg gaactccacc atctgctcct
    12601 ccgtcaacac ccccatcagc accctgtggc gcggataatg acgcaaatca agaatcgaat
    12661 aactgaacac cctcgcaatc aatgccgcaa gcgacttggg cgaaatgaca ccgtctgaaa
    12721 acagcatcgg caacacttcc ttacccgcct gcgactcatt gtagtaatgc tcggcctgct
    12781 caacagtaac cacctggttt tgaaccagaa tcctcagcaa acctacgctc atacgacctt
    12841 atccccaaat ttattcattg ttatacctgt acagctttta tagtggatta aatt
//
LOCUS       DAXHNE010000043        12867 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.043, whole genome shotgun sequence.
ACCESSION   DAXHNE010000043 DAXHNE010000000
VERSION     DAXHNE010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12867
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.043"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            389..1105
                     /locus_tag="ACFM4Z_000935"
     CDS             389..1105
                     /locus_tag="ACFM4Z_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFC6020994.1"
                     /translation="MTMHETTDRLFEIAKEQGVLKPADIAERLNISQQALKNWESRGI
                     AAKALPEVAKAFGVSETWLRTGEGSRTAPVLIDPDLPHEVKDIHRPMTWSSNDPLPDD
                     DYVFVPYLKESCFKGGVGTYEIPDYNGYRLPFGKSTLKRKGINPDNVFCCTLTGDSME
                     EKIAEDAAIAVDTGETAIRDGKIYAFAQDGMFRVKYLIRQPGNSVLIRSHNSGFYGDE
                     NAPLDSLTVIGRVFWWSVLD"
     gene            1208..1633
                     /locus_tag="ACFM4Z_000936"
     CDS             1208..1633
                     /locus_tag="ACFM4Z_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020995.1"
                     /translation="MNFLGVQGELKVHELHHRTATLCFEWLGEVSAPRRKENYKDLKP
                     NVLYDFDGSGKHFANPDARYLLPVGSSGLILKHIQIDDEDTLLRLWCARNIPMPHRLS
                     KIPMLRQYYLSKAWHEIYAINQHLRKTKLIVDVAYDPTD"
     gene            1877..2077
                     /locus_tag="ACFM4Z_000937"
     CDS             1877..2077
                     /locus_tag="ACFM4Z_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020996.1"
                     /translation="METITIPKENFDALLNLARTAVALNENFIPKINYGASTIPAAGF
                     AALNEFGIARSTVESLEILKIK"
     gene            2145..2504
                     /locus_tag="ACFM4Z_000938"
     CDS             2145..2504
                     /locus_tag="ACFM4Z_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020997.1"
                     /translation="METQEKKFVPASKQIPTVNSGWLDRELGEAMSEAVRACLAHGKQ
                     SEITVKLKIQPQNIQSGTVKISHDVATKLPKEKREGGIVFATPDGNIQADDPAQGKLK
                     LKQVPNTSSTLKMVRSN"
     gene            2529..3383
                     /locus_tag="ACFM4Z_000939"
     CDS             2529..3383
                     /locus_tag="ACFM4Z_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFC6020998.1"
                     /translation="METQENMIKTALQAAQKPFFEFAPNNTLLVFTPDQDGGWRYKSH
                     PELMQNPYRKCGKFLMHDTASLIKFVQKHKQDGTQIYIDADFKSGRIDVTAVINGHTQ
                     QAPGWLDFYAVYTPQHTTSASNWLNNNAHRMNQMEFSHFLTNNARNIVSKNPGNENSV
                     YPTAAEVLDFALNLEYTEKTTFKQGYREQDGRINFTFQSEDSGQTEKNLKMFERFGIE
                     FTPHQGGASYFVEALLKFRIDKNSGALVLWYELQQIDAVIEQAAKDISEAVQKAFPEI
                     DIYFGVIA"
     gene            3453..3668
                     /locus_tag="ACFM4Z_000940"
     CDS             3453..3668
                     /locus_tag="ACFM4Z_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6020999.1"
                     /translation="MTITEEYSIDIRVTAEQGKNDYGYPTERYGCDIINTDGELLVGI
                     EPEYKTPFAAVRQALICLTKDNLKNAA"
     gene            3703..3963
                     /locus_tag="ACFM4Z_000941"
     CDS             3703..3963
                     /locus_tag="ACFM4Z_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO1622 family putative holin"
                     /protein_id="HFC6021000.1"
                     /translation="MKYLIRTALLAVAAAGIYACQPQSEAAVQVKAENSLTAMRLAVA
                     DKQAEIDGLNAQIDAEIRQREAEELKDYRWIHGDAEVPELEK"
     gene            3960..4253
                     /locus_tag="ACFM4Z_000942"
     CDS             3960..4253
                     /locus_tag="ACFM4Z_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021001.1"
                     /translation="MNFQEENENIKNDIEINLLHSAEADSVEAMMDLAVYGLAAMVAK
                     HNKTRGEQLVLQAIAGHMRKLLAALPETESLVQTGKVYRRLEDLIFKTYLPEQ"
     gene            4256..4447
                     /locus_tag="ACFM4Z_000943"
     CDS             4256..4447
                     /locus_tag="ACFM4Z_000943"
                     /inference="COORDINATES: protein motif:HMM:NF037202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="HFC6021002.1"
                     /translation="MNTNQDTIPFGGNLVICCSTGNGGDGRFSCLITDQIPDLNLVRS
                     GQAFPMFIYEYETREKRKA"
     gene            4447..4587
                     /locus_tag="ACFM4Z_000944"
     CDS             4447..4587
                     /locus_tag="ACFM4Z_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HFC6021003.1"
                     /translation="MDTLLKIIIALSFSGAEALAVWLFITAADAVLRRFPKPSHYRRV
                     KK"
     gene            4584..4793
                     /locus_tag="ACFM4Z_000945"
     CDS             4584..4793
                     /locus_tag="ACFM4Z_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HFC6021004.1"
                     /translation="MNINELGARIDRPTIRELIAYATCRNRPISNSTLLRMEKDGRIP
                     CRLKTPLTSPVWDTREVLEALGLQQ"
     gene            complement(4851..5768)
                     /locus_tag="ACFM4Z_000946"
     CDS             complement(4851..5768)
                     /locus_tag="ACFM4Z_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFC6021005.1"
                     /translation="MNVSVLNFGNTPVSFRQDGFLNATAIASHFGKLPKDYLKSEQTQ
                     QYISALAENLSVRRKILTEANQIVIVKRGGSEQGTWLHPKLAIHFARWLNPKFAVWCD
                     EQIEILLNGKISDGIKTVTPKPTRALPDGLTGEQIEAVKKLHNALTKSAPKEAQARIA
                     ITLWSAVKSKFGCSYKEVPAEQFPEVLSVMGRVAVENGVLYGEVLDREPLPAPQPALP
                     ISGNALYDLAVAVRYGAWAIQMGRDVSLPLKQLGCKQAVTMWTVWAETRSRLKAAANA
                     LEALNAHADAEHAAKIRPMLPEIRNLSSV"
     gene            complement(6636..7115)
                     /locus_tag="ACFM4Z_000947"
     CDS             complement(6636..7115)
                     /locus_tag="ACFM4Z_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HFC6021006.1"
                     /translation="MTDNLIQIATPILTVIGVFVAAYGIMRNTENAKKRATIDMIMAE
                     RNNAALQEAITIVNGLAKTDGCILATYTSDTPDKKKDREAILTVLNQREFVCAGVLGG
                     ALHEKMYKDFEYSMLLRDWDNLSSFIFEIRRIRSAPTAFQEFEAVARKWKKKPLKTK"
     gene            complement(7551..7931)
                     /locus_tag="ACFM4Z_000948"
                     /pseudo
     CDS             complement(7551..7931)
                     /locus_tag="ACFM4Z_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(8044..9117)
                     /locus_tag="ACFM4Z_000949"
     CDS             complement(8044..9117)
                     /locus_tag="ACFM4Z_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC6021007.1"
                     /translation="MAKIITPLSANQVKNAKPRDKLYKLSDGGGLALWIYPTGGRSWK
                     LSFVQDGRQQTISLGRYPDFSLADAREWREEVRRKRAHGENVVNKKVRADFAFEKVAR
                     DWFVRWSKGRSEKYAGQVMRNFERWVFPAIGNLDIRQVRTADVVGCLRVMEARGIVDT
                     LRKTKNSLKMVFAFAVGSGMMEINPVAQIGSGVFERAKTGNMAALSPSELPRLIDFLE
                     QRNEFAVYAGRVRIHPVTRLCIYWLLLTMTRIQEAALMEWSELDGEVWRIPAERKKER
                     RGHDVPLSRAMQWVLDQARALNVNGRFVFESVNFQGHINKESPRVAMQRAGLDTTAHG
                     LRSLARTYLREVLKVDSIMRKRK"
     gene            complement(9310..9399)
                     /locus_tag="ACFM4Z_000950"
     tRNA            complement(9310..9399)
                     /locus_tag="ACFM4Z_000950"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9363..9365),aa:Ser,seq:cga)
     gene            complement(9477..9567)
                     /locus_tag="ACFM4Z_000951"
     tRNA            complement(9477..9567)
                     /locus_tag="ACFM4Z_000951"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9531..9533),aa:Ser,seq:tga)
     gene            complement(9650..10429)
                     /gene="yaaA"
                     /locus_tag="ACFM4Z_000952"
     CDS             complement(9650..10429)
                     /gene="yaaA"
                     /locus_tag="ACFM4Z_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFC6021008.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESEILMQQLRELALQ
                     QIAELMHVSDKIALLNAQRNAEWHTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKTLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            complement(10592..11779)
                     /gene="dapC"
                     /locus_tag="ACFM4Z_000953"
     CDS             complement(10592..11779)
                     /gene="dapC"
                     /locus_tag="ACFM4Z_000953"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HFC6021009.1"
                     /translation="MNTLLKQLKPYPFARLHEAMQGISAPEGMEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTVDADNEILPVLGSREA
                     LFSFVQTVLNPVSDGIKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVVDVATCVKAAETIVS
                     LYR"
     gene            complement(11855..12307)
                     /gene="dut"
                     /locus_tag="ACFM4Z_000954"
     CDS             complement(11855..12307)
                     /gene="dut"
                     /locus_tag="ACFM4Z_000954"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFC6021010.1"
                     /translation="MNIEVEMKVLDERMADFIPTYATAGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVIVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
     gene            12455..>12867
                     /locus_tag="ACFM4Z_000955"
     CDS             12455..>12867
                     /locus_tag="ACFM4Z_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFC6021011.1"
                     /translation="MTHISSPRNEFIRGIKESSPILIGLLPWALILGMQGGQKGMSWL
                     EMLLMTSMNFAGGSEFATVNLWAESLPILLIATVTFMINSRHILMGAALALHLKEIPL
                     KKAVPALFFMCDESWAMAFSEIQKRKAAGLPAFNMP"
BASE COUNT         3501 a         3307 c         2963 g         3096 t
ORIGIN      
        1 atattaaaaa ttatcgtcat caatgcggct tccccagccc gcggcgagct ttccacgaca
       61 ggcttttttt atttgggttg gtcgtctggt ttctttgagt tcaaaaacag gtcgggatac
      121 ttaagtttta ttcgtgcagg tatcccgcgc ttcgtccaat tgaaaacgca ttgagggcta
      181 ttccctgtta ttcgaccaac ttccgcgtaa ctgccgattg attgcaacag gcgtttgtct
      241 tcattgactc ttttatccat aaataaacca aatgtttaaa tctaatgcta atattaaaca
      301 ctatgtttag ataaaaatca agtcttgtgt aacaacattt tgtttaaata tgggagaata
      361 attaagccaa ccgcgaataa gattaaaaat gacaatgcac gagacaactg acagactttt
      421 tgagatagcc aaagagcagg gagttttaaa gccggctgac atagcagagc gtctgaatat
      481 cagccaacag gctttgaaaa actgggaaag tcgtggcata gcggcaaagg cgctgcctga
      541 agtagcaaaa gcattcggtg tatctgaaac atggctgaga acaggtgaag gcagccgaac
      601 cgcgcccgtc cttattgacc ccgacctacc ccacgaagtc aaagacatcc accgcccgat
      661 gacgtggagc agcaacgacc cgctgcccga cgatgattat gttttcgtcc cctacctcaa
      721 agagagctgc ttcaaaggcg gagtaggcac gtatgaaatc cccgattaca acggctaccg
      781 cctgccgttc ggcaaatcca cgcttaaacg caaaggcatc aatcccgaca acgtgttttg
      841 ctgcaccctg accggcgaca gtatggagga aaaaatcgca gaagacgcgg caattgccgt
      901 agatacgggc gaaaccgcca tacgcgacgg caaaatctac gccttcgccc aggacggtat
      961 gttccgcgtg aagtacctga tacggcagcc tggcaacagc gttctgatac gcagccacaa
     1021 cagcggtttc tatggcgacg aaaacgcccc cttggacagc ctgaccgtta tcggcagggt
     1081 attttggtgg agcgtgctgg attgaaaaat gttgctatta cgacgagata atattgatag
     1141 ggctttcaag attgtcaaaa ataggcgttt tgattcccct tggtggcctg gtgagtacga
     1201 tgccggtatg aatttccttg gcgtacaagg agagttgaag gttcacgagc tgcatcacag
     1261 aacagcaacc ctgtgctttg agtggcttgg cgaagtatct gcgccgcgca gaaaagagaa
     1321 ctacaaagac ctcaagccaa atgtattgta tgactttgat ggttccggta agcattttgc
     1381 caaccctgac gcaaggtatc tattgcccgt cggttcaagc ggcttaatct taaaacatat
     1441 ccaaatcgat gacgaagaca ctctgttgcg gctatggtgc gcacgaaata tcccaatgcc
     1501 acaccggcta tctaaaatcc cgatgcttcg gcagtattat ctaagcaagg catggcacga
     1561 aatttatgct atcaaccaac acctaaggaa aacgaagctg atagttgatg tggcatacga
     1621 ccccacagat taaacaaccc gcccgcatta tgcgggcttt tttcatgccc cgccgaacct
     1681 gaaaacaaca caaaaccgac aaagccgcct taataggcgg tttttttgcg cctgcgaaat
     1741 ctcaaaaata aattctttta ataatcaaaa tgtttataaa tcttaaacaa aaatcttaaa
     1801 cagattgttg catattatta aacgctatgt ttaaatacac ccatcgaaac aaacaacagc
     1861 cacatggagt accgacatgg aaaccatcac catccctaaa gaaaatttcg acgctttgct
     1921 aaatctcgca cgaaccgccg tcgccctgaa cgaaaatttt attccaaaaa taaattacgg
     1981 agcatccacc ataccagcag ccggctttgc cgcacttaac gagtttggaa tagctcgctc
     2041 caccgtggaa tctttggaaa ttctcaaaat taaataacct cctcggtatt gtcatcccgt
     2101 gataccggca acctaactaa cccctcttta aaggaaaatc aaaaatggaa acccaagaaa
     2161 agaaatttgt tcctgcctcc aagcaaatac cgaccgtaaa tagcggctgg ttagaccgtg
     2221 aattaggcga agccatgagt gaagcggtgc gtgcctgcct tgctcacggt aaacagtcag
     2281 aaattactgt caagctcaaa atccaacccc aaaacatcca aagcggaacg gtaaaaatca
     2341 gccacgatgt agcaaccaaa ctgcccaaag aaaaacgcga aggcggcatc gtctttgcta
     2401 cacctgacgg caacatccaa gccgacgatc cggcacaagg aaaattgaaa ctcaaacaag
     2461 ttcccaatac gtcaagcacg ttgaaaatgg tcagatccaa ctaactcaaa cccaaaagga
     2521 aatctaaaat ggaaacccaa gaaaacatga ttaaaaccgc cttacaagca gctcaaaaac
     2581 ctttttttga gtttgcaccg aacaatactc tgcttgtatt cacaccagac caagacggtg
     2641 gctggagata caaatcacac cccgaattga tgcaaaaccc gtaccgcaag tgcggcaaat
     2701 tcctcatgca cgataccgcc agtcttatta agtttgtaca aaaacacaaa caggacggca
     2761 cacaaatcta cattgatgcc gatttcaaat cagggcgtat tgatgtcacc gccgtcatca
     2821 acggccatac gcaacaagct ccgggctggc tcgattttta tgccgtatac accccgcaac
     2881 acactacatc ggcatccaac tggctgaaca acaatgcgca ccgcatgaat cagatggagt
     2941 tttcccattt cctgaccaac aatgcccgca acatcgtatc caaaaatccc ggtaacgaaa
     3001 attccgttta cccgaccgcc gccgaagtat tggatttcgc gctcaatctc gagtacaccg
     3061 aaaaaaccac cttcaaacaa ggctatcgtg aacaggacgg cagaatcaac tttaccttcc
     3121 aatccgaaga ctcagggcaa acagagaaaa acctcaaaat gtttgaacgt ttcggcatcg
     3181 agttcacccc gcatcaaggc ggtgcatcct actttgttga agccctgttg aaattccgca
     3241 tcgacaaaaa cagcggcgca ctcgtcctgt ggtacgaatt gcagcaaatc gatgctgtaa
     3301 tcgaacaagc ggcaaaagac atttccgaag ccgtacaaaa agccttccct gaaatcgaca
     3361 tctatttcgg cgtaatcgcc taaaaaaagg tcgtctgaaa ttgtgaacct tcagacgacc
     3421 tccacaaaaa cccaactccg aaagacccca aaatgaccat cacagaagaa tactccatcg
     3481 acatccgcgt aaccgcagaa caggggaaaa acgactacgg ctaccccacc gaacgctacg
     3541 gatgcgacat catcaacaca gacggggagc tgcttgtcgg catagagcca gagtacaaaa
     3601 ccccctttgc cgccgtcaga caagcgctca tctgtctgac aaaagacaac ctcaaaaacg
     3661 ctgcctgata ttaagccgtt accccaataa tgaggaatca aaatgaaata cttgatccgc
     3721 accgccttac tcgcagtcgc agccgccggc atctacgcct gccaaccgca atccgaagcc
     3781 gcagtgcaag tcaaggctga aaacagcctg accgctatgc gcttagccgt cgccgacaaa
     3841 caggcagaga ttgacgggtt gaacgcccaa atcgacgccg aaatcagaca acgcgaagcc
     3901 gaagaattga aagactaccg atggatacac ggcgacgcgg aagtgccgga gctggaaaaa
     3961 tgaactttca ggaagaaaac gagaacatca agaatgacat agagattaat ctcttgcatt
     4021 ccgctgaagc cgacagcgtg gaggcaatga tggacttggc ggtttacggg ctggcggcga
     4081 tggttgccaa acacaacaaa acgcgcggcg agcagcttgt gttacaggcg attgccggtc
     4141 atatgcggaa gcttttggcc gcgcttcccg aaaccgagtc attggtgcag accgggaagg
     4201 tttaccgccg acttgaagat ttgattttta aaacgtattt gccggagcag taaagatgaa
     4261 cacgaatcaa gatacgattc catttggcgg caaccttgtg atttgctgtt cgacgggaaa
     4321 cggcggggac gggcggtttt cttgtctgat taccgatcag ataccggatt taaatcttgt
     4381 cagaagcggg caggcgttcc cgatgttcat ttatgagtac gaaactagag aaaaaaggaa
     4441 agcctgatgg acaccctatt aaaaatcatc atcgcgctgt cattttccgg cgctgaggca
     4501 ttggcggtat ggcttttcat cacggccgcc gatgccgtat tacgccgctt cccgaaaccg
     4561 agtcattaca ggagagtaaa aaaatgaaca tcaacgaact gggcgcaagg atagaccgcc
     4621 cgaccatccg cgaactgatt gcctacgcaa cctgccgcaa ccgccccatt tcaaactcga
     4681 cactgctgcg tatggaaaaa gacggacgga taccgtgccg tctgaaaacc ccactcacat
     4741 cccccgtatg ggacacccgc gaagttttgg aagccctggg gttacagcaa taaaaaaacg
     4801 cccgattagg gcgttttcac attcagacgg cttttgtatt ccctactgca tcaaaccgac
     4861 gacaggttgc ggatttcggg cagcatcggg cggatttttg ccgcgtgttc cgcgtcggcg
     4921 tgtgcgttta aggcttcgag ggcgtttgcg gcggctttga ggcggctgcg tgtttccgcc
     4981 cagaccgtcc acatcgttac cgcctgtttg cagccgagct gcttcagcgg cagggaaacg
     5041 tctctgccca tttggatggc ccacgcgccg tatctgacgg caacggcgag gtcgtacagg
     5101 gcgttgccgc tgatgggcag ggcaggttgc ggtgcgggca atggttcgcg gtcgaggact
     5161 tcgccgtaca gcacgccgtt ttcaactgcc acgcggccca tcacgcttaa aacttcgggg
     5221 aactgttcgg caggtacttc tttgtagctg catccgaact tgcttttgac ggcagaccaa
     5281 agggtaatgg cgatacgcgc ctgcgcttct ttgggtgcgg atttggtcag ggcgttgtgc
     5341 agttttttga cggcttcgat ttgttcgccg gtcaagccgt ccggaagggc gcgggtgggt
     5401 ttgggggtaa ctgtttttat gccgtctgaa attttgccgt taagtaaaat ttcaatctgc
     5461 tcatcgcacc aaaccgcaaa tttcggatta agccaacggg caaagtgaat agcgagtttg
     5521 ggatgcagcc atgtgccttg ctcactgcca ccacgcttca cgataactat ttgatttgct
     5581 tccgttagga tttttctcct aacgcttaaa ttctcagcaa gtgcagagat atattgttga
     5641 gtttgttcac tttttaggta gtctttaggt aacttgccaa agtgagatgc aatggcggtt
     5701 gcatttaaaa aaccgtcttg acggaaagat acaggggtgt taccaaaatt gagaacagat
     5761 acgttcatga tagtttccta gtttagtttc gaaagaccca aatgggtggt cgggaggttc
     5821 gaaaacctac tagaaaaggc cggcgttatt ccccttgcgg gtattgtatt ccgcaccctc
     5881 ccgaccatag gaaactagtt cggatatatc gaaacaaaca accaaacaag aaaacttatg
     5941 gacgtaaaaa attcacgctg acggggtgaa tgccgttcta gtagaggttt tcgacgcctc
     6001 gtgtttcgta atatatccaa tctttctatt ttctgcaagc ataaaaaagc cgccatgtgg
     6061 cggatagggt cgcggtcttc aacagcatgt aggatttagg aatgcccaaa ttgggcgcag
     6121 gggtgttgaa aacacgcaaa cagacgcgcc gccagcctta cgggtaggcg cacccccaca
     6181 taggagtaaa tcgatttgat acgtatagac gtaaaaaatt ccactctatc gtgttggata
     6241 tacgctgttt gtcaggtgtt ttcaagcacc gtgggaaata ttatatagag ttatggaaat
     6301 gtgtcaagag aattagccca aatgggcgga cgcgcggtta agaacccaca taggagggcg
     6361 gacttattcc cctttcgggt cttgtattcg tcgcccacgc ggtcatagaa acttcctgct
     6421 atcgaaacaa acaacaagga aagaaaacta tagacatgaa aaaatcacat tgacggagtg
     6481 attgccgctg tagtgtggtt tctgacgcca cgaacaggaa tataaaacaa aacccctgca
     6541 catgcaaggg gtttcccaaa aaaccgtagg cggcaaactg aaaggccgtc tgaatttcag
     6601 acggcctatg ttgcggcgga tgttaagcta ttaagctatt tggttttcag aggctttttc
     6661 ttccattttc gggctacggc ttcaaattct tgaaaggccg tcggtgcgct cctgatacgg
     6721 cgtatttcaa aaatgaagct gcttaggttg tcccagtcac gtaacagcat ggagtattcg
     6781 aaatctttat acattttctc gtgcagtgct ccgcccaata cgcccgcaca gacaaattcg
     6841 cgctggttta aaactgtcag tatggcttca cggtctttct tcttgtccgg ggtatccgat
     6901 gtataggtgg cgagtatgca tccgtctgtt tttgccagcc cgtttactat ggttatggct
     6961 tcttgaaggg cggcattgtt acgttcggcc atgatcatgt cgatggtggc gcgctttttg
     7021 gcgttttctg tattcctcat gatgccgtaa gcggcaacaa aaacgccgat aacagtcaat
     7081 atcggcgttg ctatctgtat taggttgtca gtcattgccg tcccagccgt ctgaaaggcg
     7141 gaaatcacgg ctttccgaat aaatatgtcc cattgtcatc cccttaagct gatgttgaaa
     7201 ataaatcctt gcgtcggaat atacggcatt gcttgactat tttcaagatt ctgtacctcg
     7261 tgaaccggac tatacaaaaa aaccgccttc gtgtaaagcg gttcttaaat cgcccgttaa
     7321 ggcggacaca acgatagcca aactggaaca ggaggcggca aaggcgggtt tgtccaaaac
     7381 agccttgatt gaaaggctga ttggggattt gtaggcgttt gcagatacga caaggggcgc
     7441 attccgcgcc ccatcacacg cacggacggc ttgtcatcgc ctgtcgcccg tgcgcggctg
     7501 cccatatttc agaacagtct tgcaagcccc tttaagggaa cggttttatt ctagtacagt
     7561 ttgaatgcct tggcaacggc tgttttaatc gcctgaaaat cctcttcact gattattgga
     7621 atgcagcggt cgcgcccttt gggtttgtat cggtctaatc gtgccaatcc gactgttgcc
     7681 gtcatgtcgc attttgctca acattggatg tgcggcttgt ccggtaaggg gtttccactc
     7741 attttgtggt ggtagtccgc caaagggaca ggttctgtgc tgcttaaggg tacgaccgtt
     7801 accagtttgc cgttgtgcct gtttcgcgct atgacgacga cagggcgttt cttgaccatt
     7861 tccggttctt cataaccgcg aaagtcgcac atgataaccg aacgttccct tggttggaat
     7921 tttaaaggca ttagccgctc ctgacgatga cgggctgcta ttatagcagt tcttacacaa
     7981 aaaaccgcct ttgtgtaaag cggttgcaaa aaagccttcc aataaaaatg cggaaacggt
     8041 tttttatttg cgtttccgca taatactatc caccttcaga acctcgcgca aataagtacg
     8101 cgcaagcgag cgcaaaccgt gcgccgtcgt atccagcccc gcccgctgca tcgccacgcg
     8161 cggactttcc ttattgatat gcccttgaaa attcacactt tcaaacacaa accgcccgtt
     8221 cacattcaac gcccgcgcct gatccaacac ccactgcatc gcccgcgaca gcggcacatc
     8281 atgcccccgc cgctcctttt tccgttcggc ggggatacgc caaacctccc cgtccaactc
     8341 cgaccactcc atcaacgccg cctcctgaat ccgcgtcatt gtcaacagca gccaatagat
     8401 acaaagccgc gttacaggat ggatacgcac cctgcccgca taaaccgcaa attcattgcg
     8461 ctgctccaaa aaatcaatca ggcgcggcaa ttcagacgga ctcaacgccg ccatattccc
     8521 cgttttcgcc cgttcaaaca cacccgaacc gatttgcgcg acagggttga tttccatcat
     8581 tcccgaaccg accgcaaacg caaacaccat cttcagactg tttttcgttt tgcgcaacgt
     8641 atcaacgata ccgcgcgcct ccatcacacg cagacagccg accacatccg ccgtcctgac
     8701 ttgacgaata tcaagattgc cgatagccgg aaaaacccac cgttcaaaat tccgcataac
     8761 ctgtccggca tacttttcag accgaccctt cgaccaacgc acaaaccaat cacgcgccac
     8821 cttctcaaaa gcaaaatccg cccgcacctt cttattgacg acattttccc cgtgcgcccg
     8881 ttttcggcgc acctcctccc gccattcccg cgcatcggcc agcgaaaaat caggataccg
     8941 ccccagcgaa attgtctgct gccttccatc ctgcacaaac gacagcttcc aactccgccc
     9001 gcccgtcggg tagatccaca aagccaaccc gccgccgtcc gacaacttat acagcttatc
     9061 gcgcggcttc gcatttttaa cctgatttgc cgacagcggc gtaatgattt tcgccattat
     9121 ggtaatttcc cacttatcca aaaattacca tatacattac cataaaaaaa aatgcaataa
     9181 gaagataccg caagattgca aatgatttac tttgacgcgt tttgataggg caaaccgatg
     9241 atttttatat ggtaattgac acaacttgaa caaaaaagaa ccgccctgat tcagggcggt
     9301 tatgttttct ggcggaaacg gtgggattcg aacccacgga ggatttgcac cctcagcgga
     9361 tttcgagtcc gctgcattca gcctctctgc cacgtttccg ataatgaagt aaaaccaaat
     9421 aaaaatgccg atgctttatg ctacttcatt tttatttggt ttttgacccc aaggattggc
     9481 ggaagcggtg agattcgaac tcacggaggg ctatcaaccc tcgacggttt tcaagaccgt
     9541 tgcattaaac cgctctgcca cgcttccgtc ttcttgaaga agaagaataa taatgaagtt
     9601 tatacatttt gccaagcact tttttcggaa aatacttaat ttattgtttt cactttattt
     9661 gttccgaacg cataaaaacc cattcgcttt catttgaagc cgcgtcattg aatgcgtagc
     9721 cttcgtaatt aaaatttttc agcatttcag ggtcggtaat atgatgttct gccgcatagc
     9781 gcaccattaa tccgcgcgca cgcttggcat agaaactgat gattttatat ttaccgtttt
     9841 tttcgtcttt aaatatcggg gtaatcagcc gcgcccgaag tgttttcgtg ttgacggact
     9901 tgaaatattc ctgtgaggca aggttgacaa gcgtattgct gcctgcttgg gcaagcgtgt
     9961 catttaaaag gttggtaatg atgtcgcccc aaaactcata caaattcttg ccgcgcaaat
    10021 tggcaaatgc cgtccccatt tccaaacgat agggctgtat caggtctaac gggcgaagaa
    10081 gaccgtatag accggacagc aggcggacat ggttttgcag atagcgtatc tgtccaatat
    10141 ccaatgtgtt tgcatccata ccttcgtaaa catcgccgtt gaacataaag accgcctgtt
    10201 tggcgttttc cggcgtaaac ggcgtgtgcc attctgcatt gcgctgcgcg tttaagaggg
    10261 caattttgtc ggaaacgtgc atcagttcgg caatctgttg caacgcaagc tcgcgcagct
    10321 gctgcattag aatttcggac tctgccagca ggtcgggttg ggtaaactcg ctgacagggg
    10381 cagggtcttt ttcgttaagg tttttcgcag gggaaaggac aaaaaacata atctgctcgg
    10441 tattgtgtca aacacgggga ttttaagttt aaacgtgttt gcgggcaagc cgattagatt
    10501 aaaagtgaag taaataccgt tatagcaaat tcaaccgtaa aatattgaaa aatgccgtct
    10561 gaacaaaagt tcagacggca ttttttattc tttaccgata tagggaaaca atggtttccg
    10621 cagctttgac acaagttgcg acatcgacaa ccaaagcgat acgcacataa ccttccccgg
    10681 gattgccctg ttcggtatcc cgtgccaaaa aacgtccggg caatacttgg atagcggctt
    10741 tttgccataa attgcgtgca aatgccaaat cgtcgccatc ggggactttc aaccagatgt
    10801 aaaacgaggc atccggtaat ttgacgtcaa atacctgttg caaaatagga ataacgcgct
    10861 caaatttttc ctgatacagg cggcggttgt cgataacgtg ctgttcgtca tcccaggcgg
    10921 caatacttgc gcgctgcacg ggaatgctca ttgcactgcc gtgataggtt ctgtaaagca
    10981 gaaagttttt aagcagttcg gcatcgccgg cgacaaaacc ggaacgcagg cccggaacat
    11041 tggaccgctt ggacaaactg gtgaacataa gcagtttttg cctgcttcga cccaactgtg
    11101 cagcggcttg caggcagccc aaaggtttgt tgccgtcgaa atagatttcg gaatagcatt
    11161 catccgaggc aataatgaaa ccatatttat cctgtaaatc aaaaacttct ttccatccgt
    11221 ccaaatccag cacgctgccg ctggggttgt tgggcgagca gacgaacacc agtttggtgc
    11281 gtttccaaac ctcttcggaa atactgcgcc aatcggggtt gaaagacggc gcggggcaat
    11341 tggcaaaatg gatttcaccg ccgcccaaaa gtgtcgcacc ttcataaatc tgataaaagg
    11401 gattcgggct gacaattgcg ggtttgatgc cgtctgaaac agggttcaac acggtttgaa
    11461 caaaagaaaa caacgcctcc ctactgccta aaaccggcag aatttcatta tccgcatcca
    11521 ctgtcaagcc atcgtaacgg cgttttaacc agtttgcaca cgcctgacgc agttcaggca
    11581 gaccggccgt cagcggatat ttttccaact cgtgcaatga ggcggtcagc gcatccgtaa
    11641 tgactttcgg tgtcggatgt ttcggttcgc cgatgtgcag ggggacggct tccatgcctt
    11701 cgggcgcgga aatgccctgc atcgcctcgt gcagtcgggc aaagggatag ggtttgagct
    11761 gcttgagtaa ggtattcatg attttctccg gttatcggta tatcggaaga tatgccgtct
    11821 gaacctgacg tgcccttcag acggcatttt atattcaatg caaacccgta ctgccgaagc
    11881 cgccctcacc ccggctgctt ccgacaaatt cctcgacacg tttgaaacgc gcctgcacga
    11941 ttggcacgat aaccatctgc gcgatacgct caaacggttt gacagtaaac ggctcgctgc
    12001 ttctgttcca taacgaaacc ttcaattccc cttgataatc ggagtcaatc aagccgacca
    12061 aattgcccaa aacaatgccg tgtttatgcc ccaatccgga acggggcagc aaaacggcgg
    12121 catatgcggg attcgccaaa taaattgcca aacctgtcgg tacaagaaac gtttcacccg
    12181 gctgcaagac gacttcctca tccaaacagg cgcgcaagtc caaaccggca gaacccgctg
    12241 tcgcataggt agggataaaa tccgccatcc tctcgtccaa cactttcatt tctacttcaa
    12301 tattcattgc cttgctccca catacggtcg gtctgtctaa agatggtgga ttatacacaa
    12361 gccgtgttta cactgaaaca cagcaaatag gttttgtttt gaaatactcc gtctgtgtat
    12421 aatgcccgac cttcttacat atcaatatta taatatgaca cacataagct cgccccgtaa
    12481 cgaatttata cgcggcatca aagaaagttc gcccatactt atcggacttc tgccttgggc
    12541 attaatactc ggtatgcagg gcggacaaaa aggcatgagc tggctggaaa tgttgttgat
    12601 gaccagtatg aacttcgccg gcggctccga gtttgccacg gtcaacctgt gggcggaatc
    12661 tctgccgata ctgcttatcg ccaccgtaac ctttatgatt aattcgcggc atatcctgat
    12721 gggggcggca cttgccctgc acctgaaaga aataccgctg aaaaaagccg tgcccgcact
    12781 gttttttatg tgtgatgaaa gctgggcgat ggcattctcc gaaatccaaa aacggaaagc
    12841 agccggtttg cccgcattca atatgcc
//
LOCUS       DAXHNE010000044        11964 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.044, whole genome shotgun sequence.
ACCESSION   DAXHNE010000044 DAXHNE010000000
VERSION     DAXHNE010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11964
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.044"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(42..971)
                     /locus_tag="ACFM4Z_000956"
     CDS             complement(42..971)
                     /locus_tag="ACFM4Z_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6021012.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNGIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            1102..1851
                     /locus_tag="ACFM4Z_000957"
     CDS             1102..1851
                     /locus_tag="ACFM4Z_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFC6021013.1"
                     /translation="MVLRRDFLAWCDETLQTASFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            1974..2555
                     /gene="petA"
                     /locus_tag="ACFM4Z_000958"
     CDS             1974..2555
                     /gene="petA"
                     /locus_tag="ACFM4Z_000958"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HFC6021014.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            2574..3923
                     /locus_tag="ACFM4Z_000959"
     CDS             2574..3923
                     /locus_tag="ACFM4Z_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HFC6021015.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            3938..4726
                     /locus_tag="ACFM4Z_000960"
     CDS             3938..4726
                     /locus_tag="ACFM4Z_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HFC6021016.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            5065..6849
                     /gene="rmuC"
                     /locus_tag="ACFM4Z_000961"
     CDS             5065..6849
                     /gene="rmuC"
                     /locus_tag="ACFM4Z_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFC6021017.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRLQGEFADLNAQLAEKAA
                     RCDFVEQAHGKTVSELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESARLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKANKQ
                     LARDLTERANETTALSESLEYVAEDEAV"
     gene            complement(7055..7687)
                     /locus_tag="ACFM4Z_000962"
     CDS             complement(7055..7687)
                     /locus_tag="ACFM4Z_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFC6021018.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFTLPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            7779..8603
                     /locus_tag="ACFM4Z_000963"
     CDS             7779..8603
                     /locus_tag="ACFM4Z_000963"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFC6021019.1"
                     /translation="MIKKTIGGIIPIFMAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISAAVRS
                     ASSDWRGIRLHVFDVPKAQGNLYRRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRKAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTQKGTPKFATFVRVRTDR"
     gene            8672..9235
                     /locus_tag="ACFM4Z_000964"
     CDS             8672..9235
                     /locus_tag="ACFM4Z_000964"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HFC6021020.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            9316..9753
                     /locus_tag="ACFM4Z_000965"
     CDS             9316..9753
                     /locus_tag="ACFM4Z_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFC6021021.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            9822..10091
                     /locus_tag="ACFM4Z_000966"
     CDS             9822..10091
                     /locus_tag="ACFM4Z_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFC6021022.1"
                     /translation="MLKQSIEIINKLGLHARASSKFTQTASQFQSEVWVTKNGSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            10091..11866
                     /gene="ptsP"
                     /locus_tag="ACFM4Z_000967"
     CDS             10091..11866
                     /gene="ptsP"
                     /locus_tag="ACFM4Z_000967"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFC6021023.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTARRQLAERGDAFGDVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIVKVIRCEDE
                     EKSEKLIKQINSVSVEEEADFKGRK"
BASE COUNT         2874 a         3020 c         3404 g         2666 t
ORIGIN      
        1 tgaaaaaata gaaaaactgc cgggtatggt tgaataaggg gtcagaccgg ttccagttcg
       61 ctcagtccgg gcaaatctgc aaaaccgcgt tcgcgtatga tttggcaaaa attgttcaga
      121 tagctcttgt ccgtatcttc ggtacggatg gcggcataca gtttgctttg cagtccgtcg
      181 gcagtaattt ggcggtggac gacatagcct ttttcaaggt agggcatgac tgtccaatag
      241 ggaagggcgg caatgccacg tctgctggca accagttgga taatggcgat ggtcagttcg
      301 ctgtgtcggc gcggcgggtt gatgtttttc ggaatcagga tttttttggg caaatccagc
      361 atctcgtcgg gaacgggata agtaatcagg gtttccccga taaagtcttc cgccgtccaa
      421 acgtttttgg cggcaagcgg atggtctggt gcgcaaatgc cgaccatttc gtaggcaaac
      481 agcggttgga aagaaatacc gttttgtttt tccgcttcgg aaacaatggc aaggtcggca
      541 cggtgttgca gcagcagtcc gacgggatcc gcttggaatc ccgatacgat atccaattcg
      601 acttggggcc acatcgggcg gaattcgccc atggcgggca tcagccagtc gaaacaggta
      661 tggcattcga cggcaatccg cagctctccc gcctcccctt ctgtgattcg cgccaaatct
      721 cgttctgcaa cagcaacttg aggtatcagt tcgtgggcga ggcgcagcag cctttcgccc
      781 accggggtaa agcgcaaggg cgtggatttg cgttcgaaca gcggcgtgcc gtagtggttt
      841 tcgagcatac ggatctggtg ggaaagggcg gattgggtaa ggaaaacccg tttggcggca
      901 agggagacgc tgccggtttc ttcaagtgcc agcagggttt tgaggtggcg caattcgata
      961 atggaatcca tgggggcttc agacggcata ttgaaccggc gcatattaaa ataattcata
     1021 tgcgggtgca aatcggaaaa atgggcgttt cgggcatttg cggttagaat gcccgcttgt
     1081 ttggaaatga caggaaaaaa tatggttttg cgcagagatt ttttagcctg gtgcgacgaa
     1141 acgttgcaga cggcatcgtt taaagattac gcccccaatg gtttgcaggt tgaagggagg
     1201 gaatatatcg ggaaaatcgt tacgtcggta acggcaagca gggcagcgat tgattttgct
     1261 gtggagcaga aggcagattt gcttttggta catcacggta tgttctggaa aaacgagttg
     1321 ccgaccgtta ctggttggaa aaaagaacgg attgccgcac tgttacggca cgacatcaat
     1381 atggcaggct accatctgcc cctggatgca catcccacac tgggcaacaa tgcccaactc
     1441 gccgacagat tgggttttgc gacagaaaaa cggttcggcg aacaaaacct gctcaactcg
     1501 ggcagcctga aacaagccaa gacactcggc gcattggcgg cgcatattga aacagttttg
     1561 caacgtaagc ctgtcgtcat cggcaaaccc gaacgcgaaa tccgacgggt tgcatggtgc
     1621 agcggcgggg cgcaggggtt ttttcagacg gcaatagacg aaggtgtcga tctgtatttg
     1681 acgggggaaa tctctgaagc ccaataccac cttgccaatg aaacgggtac ggctttcatt
     1741 tcggcagggc atcacgcgac ggaacgttac ggcgtacgcg cgctggcaga atcggcggca
     1801 gaggttttcg ggttggaagt gtgccatttt gacgaaaaca atccggcttg agtcttgaga
     1861 aatatcataa aactttacct tattttaaat aatgctttga gtatcactgc aaactgggta
     1921 aaattgcaac gtttaatggc tcggtattcc caaaatatta ggacgactga ataatggata
     1981 atcaagaaat caacaacggc cgccgccgtt tcctgacact cgcgacctgc ggcgcgggcg
     2041 gagtggcagc attgggtgtg gcaacgccgt ttgtggccag ttttttccct tcggaaaaag
     2101 ccaaggccgc cggtgctgcc gtcgaggtgg atgtcagtaa aatcgaagcg ggtcagctgc
     2161 tgaccgccga gtggcaaggc aaaccgattt gggtgctcaa ccgtacagat cagcagctta
     2221 aagacctgaa aggcctgaac ggcgaactta ccgatcccaa ttccgatgcg gaacagcagc
     2281 cggagtatgc taaaaacgag acccgttcga ttaagccgaa catccttgtc gccatcggta
     2341 tctgcaccca tttgggctgc tcgcccacct tccgtcccga cattgcccct gccgatttgg
     2401 gtgcagactg gaaaggcggc ttcttctgcc cctgccacgg ttcgaaattc gacttggccg
     2461 gccgcgtata taaaggtgtt cctgccccga ccaacctggt tgtcccgcca tataaatact
     2521 tgagcgacac aactatcttg gtgggcgaag actaagaata aggaacgata attatggcaa
     2581 accaaaccaa tagcaaagca aaagcattgt taggctggat ggacgctcgt ttccccctgt
     2641 ctaaaatgtg gaatgagcat ttggctcaat actacgcacc gaaaaacttt aatttttggt
     2701 actacttcgg ctctttggcc ctgcttgtcc tcgtgattca aatcgtcagc ggcattttcc
     2761 tgaccatgaa ctacaaaccc gacggcaacc tgaacgccta ccatctgcct gccgccttta
     2821 ccgcagtaga atacattatg cgcgacgtgt ccggcggttg gattatccgc tatatgcact
     2881 ccaccggcgc atcgttcttc ttcattgttg tttatctgca catgttccgt ggtttgattt
     2941 acggttcgta caaaaaaccc cgtgagttgg tgtggatttt cggttctctg attttcttgg
     3001 cattgatggc ggaagccttt atgggctacc tgctgccttg gggtcagatg tccttttggg
     3061 gtgcgcaggt aattattaac ctgttctccg ccatccctgt tatcggtccg gatttgtcca
     3121 cttggattcg cggcgacttc aacgtttccg acgttaccct gaaccgcttc tttgccctgc
     3181 acgttatcgc ggtacctctg gtattgctcg gcttggttgt ggcgcacatc atcgccctgc
     3241 acgaagtggg ttctaacaac cctgacggcg ttgaaatcaa gaaaaacaaa gacgaaaacg
     3301 gtatcccgcg cgacggtatc ccgttccatc cttactatac cgttaaagac atcttgggtg
     3361 tggttgtatt cctgattgtc ttctgtgccg tattgttctt cgcaccggaa ggcggcggct
     3421 acttcctcga agcgccgaac ttcgatgcgg caaatgcgct gaaaacacct ccgcacattg
     3481 cgccggtatg gtatttcaca ccgttctacg ccattttgcg tgcgattccc tcgttcttgg
     3541 gaacgcaggt ttggggtgta atcggtatgg gcgcggcggt cgtgttgatt gccctgctgc
     3601 cttggctgga taggggcgag gtgaaatccg tccgttaccg cggcccgatt ttcaaaaccg
     3661 cattggtctt gttcatcatt tcctttatcg gtttgggtat tttgggcgcg atggtggcaa
     3721 ccgatacgcg tactttggtc gcgcgcatcc tgtccttcgt ctatttcgca ttcttcctgg
     3781 gtatgccgtt ctataccaag ctggataaaa acaaaccggt tcccgaacgc gtaaccatga
     3841 gcaccgccaa acaaaaaatc atgttctttg tttacgtcgg cattaccgcc gtgggtgctt
     3901 acctgtttgc aaccaacatc tgatgagggc agtcaaaatg aaacaagcta tgaaaaattg
     3961 gtttgctgcc ttgctgttgg cagtacctat gggtgcggca tttgcttcgg gcggtcatgc
     4021 acactatgag aaagtcgata tcgatttgcg cgaccaagtc agcctgcagc gcggcgcaca
     4081 aatctttacc aactattgcc tgtcctgcca ctcggcaagc ggtatgcgtt tcaaccgttt
     4141 gaaagacatc ggtttgactg acgaagaaat caagaaaaac ctgatgttta ccaccgataa
     4201 tgtcggcgat gtcatgcatt cggcgatgaa cccgaaagat gcggcaaaat ggtttggtgc
     4261 tgctccgccc gatttgacgt tgattgcgcg ttccaaaggt gcagactacc tttacgccta
     4321 tatgcgcggc ttctataaag atccgacccg tccgagcggc tggaacaata ctgtattcga
     4381 taaagtcggt atgccccacc cgttgtggga acagcaaggc gttcaagccg ttgagttgga
     4441 tgccaaaggt cagccggtta tggtaaaaga cgaacacggc gagatgaagc ctaagctgta
     4501 ttgggaatct accggtttgc acagccgccg tctgcctaac ggcaaagtga tccaaaaaga
     4561 gtacgacgca tatgtacgcg atttggtcaa ttaccttgtg tacatgggcg aacctgcaca
     4621 actgcaacgc aaacgtatag gctatgtcgt gatgattttc ctatttgcgg ttatgctgcc
     4681 tttggcgtat ttcctgaaaa aagaatattg gaaagacgta cactaagcgt ttggaacaaa
     4741 agggcaaatc ctttagggtt tgcccttttt tcattttgcc tgccgtttga aaagcctgaa
     4801 tccgtatgcc gtctgaacat agctgcaaca tttcagacgg catcattcta aaaatgtcag
     4861 acatcgggaa cttaatcagg gtttgtggca gattgatatt gaattaggaa aaggtctgaa
     4921 accgcaagca gcatggtaag gcgatgtcga acaggtttaa agccaatccg ctatattttt
     4981 tggagctatg tcgaacaaac aggtagaatc ggaataagcg gcggttttgt ccggtgtgaa
     5041 aatgtttgaa tattaggatg attgatggaa ctgatgactg ttttgctgcc tttggcggcg
     5101 ttggtgtcgg gcgtgttgtt tacatggttg ctgatgaagg gcaggcttca gggcgagttt
     5161 gccgatttga acgcgcaact ggcggaaaag gcggcaagat gtgattttgt cgaacaggca
     5221 cacggcaaaa ccgtgtcgga attggcggtg ttggacggga aataccggca tttgcaggac
     5281 gaaaattatg ctttgggcaa ccgtttttcc gcagccgaaa agcagattgc ccatttgcag
     5341 gaaaaagagg cggagtcggc gcggctgaag cagtcgtata tcgagttgca ggaaaaggca
     5401 cagggtttgg cggttgaaaa cgaacgtttg gcaacgcagc tcggacagga acggaaggcg
     5461 tttgccgacc aatatgcctt ggaacgccaa atccgccaaa gaatcgaaac cgatttggaa
     5521 gaaagccgcc aaactgtccg cgacgtgcaa aacgaccttt ccgatgtggg caaccgtttt
     5581 gccgcagccg aaaaacagat tgcccatttg caggaaaaag aggcggaagc ggagcggttg
     5641 aggcagtcgc ataccgagtt gcaggaaaag gcacagggtt tggcggttga aaacgaacgt
     5701 ttggcaacgc aaatcgaaca ggaacgcctt gcttctgaag agaagctgtc cttactgggc
     5761 gaggcgcgca agagtttgag cgatcagttt caaaatcttg ccaacacgat tttggaagaa
     5821 aaaagccgcc gttttaccga gcagaaccgc gagcagctcc atcaggtttt gaacccgcta
     5881 aacgaacgca tccacggttt cggcgagttg gtcaagcaaa cctatgataa agaatcgcgc
     5941 gagcggctga cgttggaaaa cgaattgaaa aggcttcagg ggttgaacgc gcagctgcac
     6001 agcgaggcaa aggccctgac caacgcgctg accggtacgc agaataaagt tcagggcaat
     6061 tggggcgaga tgattctgga aacggttttg gaaaattccg gccttcagaa agggcgggaa
     6121 tatgtggttc aggcggcatc cgtccgaaaa gaggaagacg gcggcacgcg ccgcctccag
     6181 cccgacgttt tggtcaacct gcccgacaac aagcagattg tgattgattc caaggtttcg
     6241 ctgacggctt atgtgcgcta cacgcaggcg gcggatgcgg atacggcggc acgcgaactg
     6301 gcggcacacg ttgccagcat ccgtgcacac atgaaaggct tgtcgctgaa ggattacacc
     6361 gatttggaag gtgtgaacac attggatttc gtctttatgt ttatccctgt cgaaccggcc
     6421 tacctgttgg cgttgcagaa tgacgcgggc ttgttccaag agtgtttcga caaacggatt
     6481 atgctggtcg gccccagtac gctgctggcg actttgcgga cggtggcgaa tatttggcgc
     6541 aacgagcagc aaaaccaaaa cgcgctggcg attgcggacg aaggcggcaa gctgtacgac
     6601 aaatttgtcg gctttgtaca gacgctcgaa agcgtcggca aaggcatcga tcaggcgcaa
     6661 aacagctttc agacggcatt caagcaactt gccgaagggc gcgggaatct ggtcggccgc
     6721 gccgagaaac tgcgtctgtt gggtgtgaag gcaaacaagc agcttgcacg cgatttgacc
     6781 gaacgtgcca atgaaacaac ggcgttgtcg gaatctttgg aatatgtggc agaagatgaa
     6841 gcagtctgac ttgtgcggaa aagcttgttt cagcaggggc gggaatgccg aaagcgcggc
     6901 gtaactgtac cggttctgat ttggggtttg tttgaagtac ttaccaaagc cttgtccggc
     6961 gaggtacacc ggcaaggcgg cggttgtctg agccttttgc gtttcagacg gcatcagtgc
     7021 agatgatgcc gtctgaagac cgtagggaag gcggttagaa aaacggattg tgccgctttt
     7081 cgtgtccgat ggaagtcata cgcccgtgtc cggcgacaac ttgcacggtt tcgggaaggg
     7141 tgaataattt gttgcggata ttattgatta agtcggcgtg gttgccgcgc ggaaaatcgg
     7201 ttctgcctat ggtttcgtaa aacagcacgt cgcccgcaat cagcaattcc gcctcggcac
     7261 aataaaagac gatatgtccc ggcgtatggc ccggaatatg cagcacttga aaggcatagc
     7321 gtccgaccgt gagcgtttcg ccttcttcga gccaacggtt cggcgcaaag gcgggcgaga
     7381 cgggaaatcc gtattgcgcg gtggtttgcg gcagcgattg gagcaggaat tcatcgtccg
     7441 gatgcggccc gaggacaggg actttatgcg ttttcaacat ttcgaccacg ccgcccgcgt
     7501 gatcgagatg gccgtgcgtc agccagattg ccgtgagcgt aagtttgcgg tttgccaacg
     7561 cttgcagcag gaacggcaca tcgccgccga catcggtcag gacggcttcg ccactttcgt
     7621 cgtcccaaat cagggtgcag ttttggcgga agggggtaac ggggaagatt tcgtaacgta
     7681 gggtcatcgg cgtgttccta aacggttttt cagacggcat cgggtttgcc gtttgtttta
     7741 tggcggtttg ccgcctgttt ggatattggg ggaatcgggt gattaagaag acaatcggcg
     7801 gcatcatacc gatttttatg gcggttttca tccctgcatc ggcaggcgcg gcggatttga
     7861 tgctggcgca ggaatacaaa gggcaggata ttgccggctg ggcgatgagc gagaaactcg
     7921 acggcgtgcg cgcgtattgg gacggaaagc acctgataag ccgtcagggc tatgcgttta
     7981 cgccccccaa aggttttacc gctcagtttc cgccttatcc tttggacggc gaattgtata
     8041 gcggacgcgg tcagttcgag cagatttccg ccgccgtgcg ttctgcttct tcagattggc
     8101 gcggcatccg cctgcacgtt ttcgatgtac ccaaggcgca gggcaacctc taccggcgtt
     8161 tggcagtcgc aacgcaatgg ctgaaaacgc atccgaacgc gccgattacc atcatcccgc
     8221 aaatcaaagt gcgcgaccgg cagcacgcga tggacttttt aaaacaaatc gaagcgcagg
     8281 gcggcgaagg cgtgatgctg cgtcagcccg aatcccgtta cagcggcggc aggagcagcc
     8341 aattattgaa gctgaaaagc caatacgacg acgaatgcac ggtaacgcgg cactatgagg
     8401 gcaaagggcg aaaagccgga cggttgggcg cggtcggctg caaaaaccgg cacggcgaat
     8461 tccgcatcgg cagcggtttc aaagacaaag accgcgacaa cccgcccaaa atcggcacgc
     8521 tgattaccta ccgctaccgt ggctttacgc agaaaggtac gccgaaattt gccacatttg
     8581 tgcgcgtgcg taccgaccgc tgagcaggac ggttcggacg gcagccgata ctttggttta
     8641 taattttcct tttaccgacc gattccgaca tatgaccgat ttagaaacca aacgccttga
     8701 aacacaggcg atgcttgaaa acgccgatct tttgttcgac caaggccaat gccgtgccgc
     8761 actgcaaaaa gtggcggacg agattacgcg cgatttgggc ggcaaatatc cgctgctgct
     8821 gcccgtgatg ggcggcgcgg tggtgtttac ggggcagttg ctgccgctgt tgcgttttcc
     8881 cttagatttt gattatgttc acgtttcccg ttacggcgac aagctggagg gcggcgcgtt
     8941 caactggaag cgtatgcccg atgcggaaca aatccggggc aggcacgtcg tcgtgctgga
     9001 cgatattttg gacgaagggc atacgatgtc cgccattcaa gccaaacttt tggaaatggg
     9061 tgcggcaagc tgccgtgcgg cggtgttcgc caacaagctg atcgacaaac ccaagcctat
     9121 ccgagccgat tatgtcggac tggatgtgcc gaaccgttat gttttcggtt acggcatgga
     9181 tgcggcgggc tgctggcgca atctgggcga gatttacgca ttggacggaa aataaggacg
     9241 cgatgccgtc tgaaggcctt tcagacggca tcgcgcccat acgccggtag gacaacgagg
     9301 aacaggacgc ataatatgat agggctttta atcatcacac acgaaaccat aggcgaagcc
     9361 taccgcaagc tggcacatca tttttttccg ggcggactgc ctgaaaacgt ccgcatactc
     9421 ggcgtgcaac cgacggaaga ccaagacgac atcatcaaca acgccattgc cgcgcttcag
     9481 gagtttcccg acaacgacgg cgtgctgatt atgaccgata ttttcggcgc gaccccctgc
     9541 aatgcagccc gccgcctcgt gcgcgaaaac aaatcggcga ttttgaccgg gctgaacgcg
     9601 ccgatgatgg ttaaggccgt ccaatattcg ccggcggcgg aagaccttgc cgcctttacc
     9661 gaatgcgtca gggaggcggc ggtaaaaggc attttcgcca tcacgtccgc gcccgaagat
     9721 ttggtgtgcc ggcgcagcgg cgatgccgtc tgaagaagcg gcagggcagg aaaacatttt
     9781 aaacggcgtc tgctgccgat atacataaca cgggaatcga aatgctcaaa caatccatcg
     9841 aaatcatcaa caaactcgga cttcacgccc gtgcctccag caaattcacc caaaccgcgt
     9901 ctcagtttca aagcgaagtc tgggtaacga aaaacggcag ccgcgtcaac ggcaaaagca
     9961 tcatggggct gatgatgctc gccgccgcca agggtacggt catcgaactg gagacggacg
    10021 gcgcggacga ggcggaagcg atgcgcgccc tgaccgactt aatcaacggc tacttcggcg
    10081 agggcgaata atgagtatcg tgctgcacgg cgtggcggcg ggcaaaggca ttgccgtcgg
    10141 ttgcgcccac ctgattgcgc gcggtacgga ggaagtgccg cagtatgatg ttgcggaggc
    10201 ggacaccgat gccgaagccg aacgtttcga tgccgccgtc aaagccacgc gcaaagagtt
    10261 ggaacagctc cgcagcgcaa ttcccgaaaa cgccccgacc gagttgggcg cgttcatctc
    10321 gctgcacctg atgctcttga ccgatgttac cttgtcgcgc gaacccgtcg atattttaag
    10381 ggaacaaaaa atcaacgccg agtgggcatt gaagcagcag agcgacaaac tcgccgccca
    10441 attcgacaat atggacgatg cctatttgcg cgaacgcaag caggatatgc tgcaagtcgt
    10501 ccgccgcatc cacaacaacc tgatcgggca gggcaacgag ttggaagttg ccgacaacct
    10561 gtttgacgaa accgttctga ttgcaaacga cctttcgccc gccgacacgg ttttgtttaa
    10621 agagcagcgc attgccgcct tcgttaccga tgccggcggc cccaccgggc atacggcgat
    10681 tttgggcagg agcttggaca tcccgtccgt cgtcgggctg cacaacgcgc gcaaactgat
    10741 taccgaaggc gaaacggtca ttgtggacgg tatcaacggc gtgttgatta tcgcgccgga
    10801 tgagtcggtg ttgaacgaat accgccgccg tgcccgcgaa taccgcagcc acaaacgcga
    10861 tttgaacaag ctcaaaaaaa ccgccgccgc caccgccgac ggggtctgca tcgagcttgt
    10921 gggcaatata gaatccgccg aagacgtgaa accgctgcac aacctcggcg cagacggcat
    10981 cgggctgttc cgcagcgagt ttctttacct gaaccgcgat acgatgccgt ctgaagacga
    11041 gcagtacgaa gtgtacagcg cgattgtcaa aaaaatgaag ggcaaaagcg tgaccatccg
    11101 caccgttgat ttgggcgtgg ataaaaaccc ccgctggttc gggaaaaaca gcacgcccaa
    11161 cggcagcctc aaccccgcgc tgggcatgac cggcatccgc ctgtgccttg ccgaaccggt
    11221 catgttccgc acccagatgc gcgccatcct ccgtgcggcg gtacacggcc ctgtgcggat
    11281 gatgtggccg atgattacct ccgtatccga agtgcgccag tgcctgattc atttggacac
    11341 cgcccgccgc cagcttgccg aacgcggcga tgccttcggc gatgtcggca tcggctgcat
    11401 gattgaaatt ccgtctgccg cgctgaccgt cggcagtatt ttgaaactgg tcgatttcat
    11461 ctccgtcggt accaacgacc tgattcaata catcttgtcc gtcgatcgcg gcgacgacag
    11521 cgtcagccat ctttaccagc ccggacatcc cgccgtgctg aaaatgctgc aacacgtcat
    11581 ccgcaccgcc aaccgcatgg acaaagacgt gtccgtatgc ggcgagatgg cgggcgatac
    11641 cgcgtttacc cgcgttttat tgggtatggg gctgcgccgt ttttccatga accccaacaa
    11701 catcctgccc gtcaaaaaca tcattctgca cagcaatgtc gggcagctcg aaagcgatat
    11761 tgtgaaagtc atccgctgcg aagacgaaga gaagtcggaa aagctgatca aacagatcaa
    11821 cagcgtgtct gtcgaggaag aagccgactt caaagggcgg aaataaatac ggcaggtgaa
    11881 aaatagaaat acttaacaat gcccgcaatc tgaaattttg ccattcttgc aaaatagaaa
    11941 accgaaacag aaacccaaaa tcgg
//
LOCUS       DAXHNE010000045        11798 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.045, whole genome shotgun sequence.
ACCESSION   DAXHNE010000045 DAXHNE010000000
VERSION     DAXHNE010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11798
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.045"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            258..1253
                     /gene="fbpA"
                     /locus_tag="ACFM4Z_000968"
     CDS             258..1253
                     /gene="fbpA"
                     /locus_tag="ACFM4Z_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HFC6021024.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALITYSGAAVLKSSQNKDEAKKFVAFLAGKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            1321..2838
                     /gene="fbpB"
                     /locus_tag="ACFM4Z_000969"
     CDS             1321..2838
                     /gene="fbpB"
                     /locus_tag="ACFM4Z_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HFC6021025.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            2859..3917
                     /gene="fbpC"
                     /locus_tag="ACFM4Z_000970"
     CDS             2859..3917
                     /gene="fbpC"
                     /locus_tag="ACFM4Z_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HFC6021026.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPAASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGSALFFPGNTL"
     gene            4102..5604
                     /gene="pta"
                     /locus_tag="ACFM4Z_000971"
     CDS             4102..5604
                     /gene="pta"
                     /locus_tag="ACFM4Z_000971"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFC6021027.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFSRQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            5735..6373
                     /gene="hisH"
                     /locus_tag="ACFM4Z_000972"
     CDS             5735..6373
                     /gene="hisH"
                     /locus_tag="ACFM4Z_000972"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFC6021028.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDPQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            6406..7143
                     /gene="hisA"
                     /locus_tag="ACFM4Z_000973"
     CDS             6406..7143
                     /gene="hisA"
                     /locus_tag="ACFM4Z_000973"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFC6021029.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVARDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            7156..7923
                     /gene="hisF"
                     /locus_tag="ACFM4Z_000974"
     CDS             7156..7923
                     /gene="hisF"
                     /locus_tag="ACFM4Z_000974"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFC6021030.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNSENTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRAMREAGIEVRL"
     gene            7954..8349
                     /gene="hisI"
                     /locus_tag="ACFM4Z_000975"
     CDS             7954..8349
                     /gene="hisI"
                     /locus_tag="ACFM4Z_000975"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HFC6021031.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDTIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            8456..10051
                     /locus_tag="ACFM4Z_000976"
     CDS             8456..10051
                     /locus_tag="ACFM4Z_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFC6021032.1"
                     /translation="MFQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPISMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATVRMVEPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            10070..10258
                     /locus_tag="ACFM4Z_000977"
     CDS             10070..10258
                     /locus_tag="ACFM4Z_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor"
                     /protein_id="HFC6021033.1"
                     /translation="MPSKPEKRASDGIFACNSNADGQNQGASDTVIGHPVRMNLGLTQ
                     ERWPDIVFHETREHSVKL"
     gene            10277..10393
                     /locus_tag="ACFM4Z_000978"
     CDS             10277..10393
                     /locus_tag="ACFM4Z_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HFC6021034.1"
                     /translation="MPSEPEKRASDGIFACNSNADDQNQGASDTVIGCVPSA"
     gene            complement(10427..10957)
                     /locus_tag="ACFM4Z_000979"
     CDS             complement(10427..10957)
                     /locus_tag="ACFM4Z_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFC6021035.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGERSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDEVMIGAGSLVPPRKRLEGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(10998..>11798)
                     /locus_tag="ACFM4Z_000980"
     CDS             complement(10998..>11798)
                     /locus_tag="ACFM4Z_000980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6021036.1"
                     /translation="RTPNIKYIYQDNQGLSEARNTGIKNSNGKYIVFIDSDDFINCQV
                     LLDFLSKDDIDMPDVVFLNAVKYDKGSVSYFGEDYQPEKILNQSKVEVLKGLCRFRKF
                     PGSAWNKIIKRELIIKEKLFFEKGIYSEDIEWSMRLFNAATTFFYLDGCYYYYRQGRK
                     DSITGTVSEKSIKSLLYILEKNAEMEFNRDISSYLYSFLSYEYLVLLFIMTSKNIECD
                     ADIKRRAYHLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKVIRGNV"
BASE COUNT         2906 a         3580 c         2799 g         2513 t
ORIGIN      
        1 gttttaaaga gaggggttct caagcattcc gacaaatcaa aatgccgcct ttacccacat
       61 tccgccacgc attgcccgcc acttccgcct tacgggcgag actcacggaa aaaatacaag
      121 tcagaacaaa caataaccgg atcggacaac aacaaattta aaaaataatt atttgcattt
      181 ataaaaaaga aggaatacac tacacaccgt tatgtcttaa caaggtatac actctcattc
      241 attaggagaa aatcgatatg aaaacatcta tccgatacgc actgcttgcc gcagccctga
      301 ccgccgcaac ccccgcgctg gcagacatta ccgtgtacaa cggacaacac aaagaagcag
      361 cacaagccgt tgcagatgcc tttacccggg ctaccggcat caaagtcaaa ctcaacagcg
      421 ccaaaggcga ccagcttgcc ggtcaaatca aagaagaagg cagccgaagc cccgccgacg
      481 tattctattc cgaacaaatc ccggcactcg ccaccctttc cgcagccaac ctcctagagc
      541 ccctgcccgc ctccaccatc aacgaaacac gcggcaaggg cgtgccggtt gccgccaaaa
      601 aagactgggt ggcactgagc ggacgttcgc gcgtcgtcgt ttacgacacc cgcaaactgt
      661 ctgaaaaaga tttagaaaaa tccgtcctga attacgccac gccgaaatgg aaaaaccgca
      721 tcggttacgc ccccacttcc ggcgcgttct tggaacaggt tgtcgccatc gtcaaactga
      781 aaggcgaagc ggccgcattg aaatggctca aaggtctgaa agaatacggc aagccttacg
      841 ctaaaaactc cgtcgccctt caagcggttg aaaacggcga aatcgatgcc gccctcatca
      901 acaactacta ctggcacgct ttcgcgcgtg aaaaaggcgt acaaaacgtc cacacccgcc
      961 tgaatttcgt ccgccacaga gatcccggcg cactcattac ctattccggt gcagccgtgt
     1021 taaaatcctc ccaaaacaag gatgaggcga aaaaattcgt cgccttcctc gccggcaagg
     1081 aaggacagcg tgccctgacc gccgtccgtg ccgagtatcc tttgaatccg cacgtggtat
     1141 ccaccttcaa tttggaaccc atcgccaagt tggaagcacc ccaagtgtcc gccaccactg
     1201 tttccgaaaa agaacacgcc acccggctgc ttgagcaagc cggtatgaaa taagccgttt
     1261 tcggattgtc aaacgggtgg acatttatac tgtccgcccg ttttgccgat aaaaaacact
     1321 atgtctccta aaaaaatacc catttggctt accggcctca tcctactgat tgccctaccg
     1381 cttaccctgc cttttttata tgtcgctatg cgttcgtggc aggtcggcat caaccgcgcc
     1441 gtcgaactac tgttccgccc gcgtatgtgg gatttgctct ccaacacctt gacgatgatg
     1501 gcgggcgtta ccctgatttc cattgttttg ggcattgcct gcgccctttt gttccaacgt
     1561 taccgcttct tcggcaaaac cttttttcag acggcaatca ccctgccttt gtgcatcccc
     1621 gcatttgtca gctgtttcac ctggatcagc ctgaccttcc gtgtcgaagg cttttggggg
     1681 acagtgatga ttatgagcct gtcctcgttc ccgctcgcct acctgcccgt cgaggcggca
     1741 ctcaaacgca tcagcctgtc ttacgaagaa gtcagcctgt ccttgggcaa aagccgcctg
     1801 caaacctttt tttccgccat cctcccccag ctcaaacccg ccatcggcag cagcgtgtta
     1861 ctgattgccc tgcatatgct ggtcgaattt ggcgcggtat ccattttgaa ctaccccact
     1921 tttaccaccg ccattttcca agaatacgaa atgtcctaca acaacaatac cgccgccctg
     1981 ctttccgctg ttttaatggc ggtgtgcggc atcgtcgtat ttggagaaag catatttcgc
     2041 ggcaaagcca agatttacca cagcggcaaa ggcgttgccc gtccttatcc cgtcaaaacc
     2101 ctcaaactgc ccggtcagat tggcgcgatt gtttttttaa gcagcttgtt gactttgggc
     2161 attattatcc cctttggcgt attgatacat tggatgatgg tcggcacttc cggcacattc
     2221 gcgctcgtat ccgtatttga tgcctttatc cgttccttaa gcgtatcggc tttaggtgcg
     2281 attttgacta tattatgtgc cttgcccctt gtttgggcat cggttcgcta tcgcaatttt
     2341 ttaaccgttt ggatagacag gctgccgttt ttactgcacg ccgtccccgg tttggttatc
     2401 gccctatcct tggtttattt cagcatcaac tacacccctg ccgtttacca aacctttatc
     2461 gtcgtcatcc ttgcctattt catgctttac ctgccgatgg cgcaaaccac cctgaggact
     2521 tccttggaac aactccccaa agggatggaa caggtcggcg caacattggg gcgcggacac
     2581 ttctttattt tcaggacgtt ggtactgccg tccatcctgc ccggcattac cgccgcattc
     2641 gcactcgtct tcctcaaact gatgaaagag ctgaccgcca ccctgctgct gaccaccgac
     2701 gatgtccaca cactctccac cgccgtttgg gaatacacat cggacgcaca atacgccgcc
     2761 gccacccctt acgcgctgat gctggtatta ttttccggca tccccgtatt cctgctgaag
     2821 aaatacgcct tcaaataaca gcttgaggaa gtaccgccat gaccgccgcc ctgcacatcg
     2881 gacacctgtc caaaagtttt caaaacaccc cggttttaaa cgacatttcg ctcagcctcg
     2941 acccgggcga aatcctcttt atcgtcggcg cgtccggctg cggcaaaacc acccttttac
     3001 gctgccttgc cggttttgaa caacccgatt ttggagaaat ttcgctttcc ggcagaacca
     3061 tcttctcgaa aaataccaac ctccccgtcc gcgaacgccg tttgggttat gtcgtacagg
     3121 aaggtgtact gtttccccac ctgaccgttt accgcaacac cgcctacggg ctgggcaacg
     3181 ggaaaggcaa gacggcgcaa gaacggcagc gcatcgaagc tatgttggaa ttgaccggca
     3241 tttccgaact tgccggacgc tatccgcacg aactttcggg cggacagcaa cagcgcgtcg
     3301 ccctcgcccg cgccctcgca cccgatcccg aactgatttt gttggacgaa cccttcagcg
     3361 cgctggacga acagttgcgc cgccagattc gcgaagacat gattgccgcc ctgcgcgcca
     3421 acggcaaatc tgccgttttc gtcagccacg accgcgaaga agccctgcaa tacgccgacc
     3481 ggattgccgt gatgaaacag gggcgcatcc tccaaactgc aagccctcac gaattgtacc
     3541 gacaacctgc cgaccttgat gccgccctgt ttatcggcga aggcatcgtg ttccccgccg
     3601 cgctcaacgc cgacggcacc gccgattgcg gattgggccg cctgcccgtt caaagcggcg
     3661 cacccgcagg cacacgcggc acactgctca tccgtccgga acagttcagc cttcaccccc
     3721 attccgcacc cgccgcctcc attcacgccg tggttctcaa aaccacgccc aaagcgcggc
     3781 ataccgaaat cagcctccgg gtcggacaaa ccgtcctcac gctcaacctc ccttccgccc
     3841 ccaccctgtc agacggcatt tccgccgtcc tccatttgga cggttccgcc ctgttcttcc
     3901 ccggaaatac cctctgaaag gcggcagcat ccacaagcct gcggatattt atcttgttgg
     3961 aaacagaatt tgtttgctat attcaacctg cgcgcctcaa gccaaacacg gcacgcacgg
     4021 cacgcaggca gccgtttctg cctatatgcc gccccttcca accacatatg ccgcacaccg
     4081 cagcatacga aaggatatat catggcaaaa gtactcatcg tacccgtatc tgccggactg
     4141 gacgcctccg ccgccgcaca agcctttgca aaagcactgg acgcacaaat tttccaagcc
     4201 gttgacgcaa ccgccgaaac cctgctcgcg caaggcaaaa gcgatgactg gttcgacgca
     4261 ctggtcggca aagttgccgc actcgatgcc gccaacctcg tcatcgaagg catcgcgccc
     4321 gatgccgaca aaatctacct cgcaggcaaa aacgtcgaac tggcattgtc ccttgatgcg
     4381 gcagccgtct tcgccgtccg ttccgacaac gccgatgccg acgaactggc aaatcggctg
     4441 aaccttgcca aacagttctt cgccgccgcg ccgggcgtat tggaaggttt tgtcgtggac
     4501 ggcgcggcag cctccgttgc cgaagcggca gccgaaaaaa ccggcctgac cttcttcggt
     4561 tcgagcgacg cgctgaaaga cgtatccgta ttggcaggcc gcgaagcaaa acgcctgtcg
     4621 ccggcgcaat tccgctacaa cctgatcgac ttctcccgcc aagccgacaa acgcatcgtc
     4681 ctgcctgagg gcgcagaacc ccgcaccgtc caagccgccg ccatctgcca cgaaaaaggc
     4741 attgcccgct gcgtcctgct tgccaaacgc gaagaagtcg aagccgttgc caaagaacgc
     4801 ggcatcagcc tgcccgactc tttggaaatc atcgatcccg cctcattggt cgaacaatac
     4861 gtcgagccaa tgtgcgaact gcgcaaatcc aaaggcctga cacccgaaga cgcgcgcaag
     4921 caactgcaag acaccgtggt actcggtacg atgatgatgg cgcaaaatga tgtggacggt
     4981 ttggtatccg gtgcggttca caccaccgcc aacaccatcc gccccgcttt gcaactgatt
     5041 aaaaccgcac cgggcgcaag cctcgtgtcc agcgtattct ttatgctgct gcccaaccaa
     5101 gtcctcgtct tcggcgactg cgcggttaat ccgaacccga ccgcgcaaca gcttgccgac
     5161 atcgccatcc agtctgccga ttccgcaaaa gccttcggca tcgacccgaa agtggcgatg
     5221 atttcctact ccaccgtcaa ctccggcagc ggccccgatg tcgataccgt catcgaagca
     5281 accaaacttg cccgggaaaa acgccccgac ctcgccatcg acggcccgct gcaatatgat
     5341 gcggcaaccg tgccgggcgt gggcaaatcc aaagccccgg gcagcccggt ggcaggacag
     5401 gcaaccgttt tggtcttccc cgacctgaac accggcaact gcacctataa agccgtccaa
     5461 cgcagtgcca acgtcttgag cgtcggcccg ctgctgcaag gcctgcgcaa accggtcaac
     5521 gacctgtcgc gcggcgcgct ggtggaagac atcgtgttca ccatcgccct gactgccgtt
     5581 caggcaaaac aaatggaagg ctgacaaacg gctttccggg tttgaaccct atgccgtctg
     5641 aagacagatt cccgttttca gacggcattt ttatcagccc ggcacatttg tttgttaaaa
     5701 tcgcagccat attgcaaaaa aagaggagga aaccatgcaa accgccatta tcgattacgg
     5761 tatgggcaac ctgcattccg tattgaaatc cgtccggacg gcggggcagc ttgccggaaa
     5821 aaataccgaa atctttttaa gcggcgaccc cgaacgcgtg tcccgcgccg acaaagtcat
     5881 ttttcccggg cagggcgcga tgcccgactg tatggcggca ctgacgcgcg gcggcctgga
     5941 cgaggcagtc aaagatgcct taaaaaacaa accctttttc ggaatctgcg tcggcgcgca
     6001 acttttattc gaccacagcg aagaagggaa caccgacggc ttgggctggt tcggcggcaa
     6061 agtcagacgc tttgagcgcg acctccgcga cccgcaggga tgccgtctga aagtcccgca
     6121 tatgggctgg aacaccgtgc gccaaaccca aaaccacccg ctgtttaaag atattcccca
     6181 agacacgcgt ttttacttcg tccacagcta ctatttcgcc cccgaaaatc ccgaaaccat
     6241 attgggcgaa agcgactacc cgtccccgtt tgcctgcatc gtcggcaaag acaacgtatt
     6301 cgccacgcaa tttcacaccg aaaaaagcca cgatgccggg ctgacgatgt tgaaaaactt
     6361 tttaaactgg taagccggac acggccccgc acaaggaaaa aaactatgct gctgataccc
     6421 gccatcgatt tgaaagaagg acgctgcgtc cgcctgaaac aagggctgat ggaagaggcg
     6481 accgtctttt ccgattcgcc cgccgaaacc gcgctgcact ggttcaaaca gggcgcgcgc
     6541 cgcctgcatc tggtagattt gaacggcgcg tttgccggtg ttccgcaaaa cctgcccgcc
     6601 atcaaagaca tccttgccgc cgtcgcccga gacatccccg tacagctcgg cggcggcata
     6661 cgcgatttga aaaccatcgg acaatatttg gatttgggct taaacgacgt gattatcggc
     6721 acggcggcgg tcaaaaaccc cgacttcgtg cgcgaggcgt gcaaagcctt ccccggcagg
     6781 attatcgtcg gactggatgc caaagacggt atggccgcca tcgacggctg ggcaaccgta
     6841 accgggcatc atgtaattga tttggcaaaa cgctttgaag acgacggtgt caacagcatc
     6901 atctacaccg acatcgggcg cgacggtatg atgagcggcg tgaacatcga cgcgacggtc
     6961 aaactcgccc aagccgtccg cattcccgtc atcgcctccg gcggactgac cggcttggac
     7021 gacatccgcg ccctgtgtgc cgccgaaaaa catggcgtag caggcgcgat taccggccgc
     7081 gcgatttacg agggcagcat cgattttgcc caagcgcagc aactggcaga ttccctcgac
     7141 taaaggcatc cgattatggc actggcaaaa cgcatcatcc cctgtctcga cgtaaaagac
     7201 gggcgcgtcg tcaaaggcgt gaacttcatc ggtttgcgcg acgcgggcga ccctgtcgaa
     7261 gccgccaaac gctacaacgg cgaaggcgcg gacgaattga ccttcctcga catcaccgcc
     7321 tcatccgaca accgcgacac catcctgcac atcatcgaag aggttgccgg acaagtcttc
     7381 atccccctga ccgtcggcgg cggcgtacgc accgttgccg acatccgccg cctgctcaat
     7441 gccggcgcgg acaaagtcag catcaacacc gccgccgtta cccgtcccga cctgattgac
     7501 gaagccgccg gatttttcgg ttcgcaagcc atcgtcgccg ccgtcgatgc caaagccgtc
     7561 aactccgaaa acacacgctg ggaaatcttt acccacggcg ggcgcaatcc gaccggtttg
     7621 gatgcggtgg aatgggcgat cgaaatgcaa aaacgcggcg cgggcgaaat cctgcttacc
     7681 ggtatggaca gggacggtac gaaacagggt ttcaacctgc cgctgacccg cgccgttgcc
     7741 gaagccgtcg acatccccgt catcgcctcc ggcggggtcg gcaatgtccg gcacctgatt
     7801 gaaggcataa ccgaaggcaa agccgatgcc gtacttgccg ccggcatttt ccatttcggg
     7861 gaaatcgcca tccgcgaagc caaacgcgct atgcgcgaag ccggcatcga agtgcgcctc
     7921 tgaccgcccc gactatgccg tctgaaagga aatatggata aaaacctgct tgaagccgtc
     7981 aaatttgacg aaaaaggttt ggtttgcgcc atcgcccaag atgccgaaac caaacgtgtt
     8041 ttaatggtgg cgtggatgaa cgccgaagcc ctgcaaaaaa ccgtcgaaac cggctttgcc
     8101 cactattaca gccgttcgcg ccaaaaacaa tggatgaagg gcgaagagtc gggacacacg
     8161 caaaaagtcc gcgaactgcg cctcgactgc gacggcgaca ccattgtgat gctcatcgcc
     8221 caaaacggcg gcatcgcctg ccacaccggg cgagaaagct gcttttacaa agtctggcgt
     8281 ggcagcgcgt gggaaaccgc cgatgccgtc ctgaaagacg aaaaagagat ttacggcagc
     8341 acgcactgac cgcctccaac attgaattat caggcatttt tttgtacaat ttcgccgtct
     8401 caaacactgt ccgggccgtc tgaaaagcgg cctgaacctt tttgcaaaga aaaccatgtt
     8461 ccaagaaatc ctcgaccaag tgcgccgccg ccgcacgttt gccatcatct cccaccctga
     8521 cgcaggtaaa accacgttga ctgaaaaact cttgctgttt tcaggtgcga ttcaaagcgc
     8581 gggtacggta aaaggcaaaa aaaccggcaa attcgccact tccgactgga tggacatcga
     8641 gaagcagcgc ggcatttccg tggcatcaag cgtgatgcag ttcgactata aagaccacac
     8701 cgtcaacctc ttggacacgc cgggacacca agatttctca gaagacacct accgcgtttt
     8761 gaccgccgtg gacagcgcgt tgatggtcat cgacgcggca aaaggcgtgg aagcgcaaac
     8821 catcaagctc ttaaacgtct gccgcctgcg cgatacgccg attgttacgt tcatgaacaa
     8881 atacgaccga gaagtacgcg attcgctgga actcttggac gaagtggaaa acatcctgca
     8941 aatccgctgc gcgcccgtta cctggccgat tagtatgggc aaaaacttca aaggcgtata
     9001 ccacatcctg aacgatgaaa tctatctctt tgaagccggc ggcgaacgtc tgccgcacga
     9061 attcgacatc atcaaaggta tcgataatcc tgaattggaa caacgctttc cgttggaaat
     9121 ccaacagttg cgcgacgaaa tcgaattagt gcaggcagca tcgaacgagt ttaatctcga
     9181 cgaattcctt gcaggcgaac tcacgcccgt gtttttcggt tcggcgatta acaacttcgg
     9241 tattcaggaa atcctcaatt cattgattga ctgggcgccc gcgccgaaac cgcgcgacgc
     9301 gaccgtacgt atggtcgagc cggacgagcc gaagttttcc ggatttatct tcaaaatcca
     9361 agccaatatg gacccgaaac accgcgaccg tattgccttc ttgcgcgtct gctccggcaa
     9421 attcgagcgc ggcatgaaga tgaaacacct gcgtatcaac cgcgaaatcg ccgcctccag
     9481 cgtggttacc ttcatgtcgc acgaccgcga gctggttgaa gaagcctacg ccggcgacat
     9541 tatcggcatc ccgaaccacg gcaacatcca aatcggcgac agcttctccg aaggcgaaca
     9601 actggcgttc accggcatcc cattcttcgc acccgaactg ttccgcagcg tacgcatcaa
     9661 aaacccgctg aaaatcaaac aactgcaaaa aggcttgcaa cagctcggcg aagaaggcgc
     9721 ggtgcaggtg ttcaaaccga tgagcggcgc ggatttgatt ttgggcgcgg tcggcgtgtt
     9781 gcagtttgaa gtcgttacct cgcgccttgc caacgaatac ggcgtggaag ccgtgttcga
     9841 cagcgcatcc atctggtcgg cgcgctgggt atcgtgcgac gacaagaaaa aactggcgga
     9901 atttgaaaaa gccaacgcgg gcaacctcgc catcgacgcg ggcggcaacc tcgcctacct
     9961 cgcccccaac cgcgtgaatt tgggactcac gcaagaacgc tggccggaca tcgtgttcca
    10021 cgaaacgcgc gagcattcgg tcaaacttta aaaagcaatc ggcaataaaa tgccgtctaa
    10081 acccgaaaaa agggcttcag acggcatttt tgcctgcaac tcaaacgcag acggtcaaaa
    10141 tcaaggcgca tcggataccg ttatcgggca tcccgtccgc atgaatttag ggctgacgca
    10201 agaacgttgg ccagacatcg tgttccacga aacgcgcgag cattcggtca aactttaaaa
    10261 agcaatcggc gataaaatgc cgtctgaacc cgaaaaaagg gcttcagacg gcatttttgc
    10321 ctgcaactca aacgcagacg atcaaaatca aggcgcatcg gataccgtta tcggatgcgt
    10381 cccgtccgcg tgaatttggg gctgacgtag gaacgccgat gtgatttcac atcccgtact
    10441 gtttcgacag cttcacataa tgcgcggcgg aatatttcaa aaaggctttt tcctcatcgg
    10501 tcagcacgcg cacttgtctg accggcgaac cgacataaag atagccaccc tccaaacgtt
    10561 tacgcggcgg aacgaggctg cccgcgccaa tcatcacttc gtcctcaatc acagcatcgt
    10621 ccaaaacggt gctgcccata ccgaccaaga cgcggttgcc gattcggcag ccgtgcagca
    10681 tcactttgtg ccccacggta acgtcttcgc cgataaccag cggcgaccct tcgggtttgg
    10741 cggcggtttt gtgggaaacg tgcaggacgc tgccgtcctg tatattgctg cgctcgccga
    10801 cggtgatgct gttcacatcg ccgcgcaaca cggcgcacgg ccacacggaa acgtcttcgg
    10861 caagcgacac ttcgccgatg acgacgcacg cttcgtctat catacagctt tcgtggattt
    10921 cgggcgtgcg gttttggaaa gttcggattg cgttcatttt tcctccttcg gtaagatgta
    10981 tagtgttaaa ggatttatta aacattccct ctgattactt ttaaaatcct gcctgtaata
    11041 tcgaccccga ataatgtgat tatcgggaat atcagcttat atatcaattt attggacttt
    11101 aacagcataa accttaaatg atacgccctt ctttttatat cagcatcaca ctctatattt
    11161 ttactcgtca tgataaaaag caaaacgaga tattcgtagg aaagaaagga ataaagataa
    11221 cttgatatat ccctattaaa ttccatttcc gcatttttct ccaaaatata taataatgac
    11281 tttatacttt tttccgaaac agttcccgta atagaatctt ttcttccctg ccgataatag
    11341 taataacaac cgtccaaata aaaaaaagtt gttgccgcat taaataacct cattgaccat
    11401 tcgatatctt cagaataaat ccccttttca aaaaatagtt tttctttgat gatcaattct
    11461 ctttttataa tcttattcca cgccgaaccc ggaaattttc taaatcggca taatcctttc
    11521 aaaacttcga ctttggattg attgagtatt ttttcaggct gataatcttc gccaaaatat
    11581 gaaacacttc ccttatcata tttaaccgca tttaaaaaaa ccacatccgg catatcaata
    11641 tcatctttac taagaaaatc cagcaagacc tgacaattaa taaaatcatc cgaatcaata
    11701 aagactatat attttccgtt tgaatttttt attccggtat ttctcgcctc tgacaatcct
    11761 tggttatcct gatatatata tttgatattt ggtgtccg
//
LOCUS       DAXHNE010000046        11742 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.046, whole genome shotgun sequence.
ACCESSION   DAXHNE010000046 DAXHNE010000000
VERSION     DAXHNE010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11742
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.046"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(147..1190)
                     /gene="argC"
                     /locus_tag="ACFM4Z_000981"
     CDS             complement(147..1190)
                     /gene="argC"
                     /locus_tag="ACFM4Z_000981"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFC6021037.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPDLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEVGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETILRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLDAIPLLP"
     gene            complement(1187..1468)
                     /locus_tag="ACFM4Z_000982"
     CDS             complement(1187..1468)
                     /locus_tag="ACFM4Z_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021038.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSTANQNPVGQRSDGIPANHTKEKP"
     gene            1836..2990
                     /locus_tag="ACFM4Z_000983"
     CDS             1836..2990
                     /locus_tag="ACFM4Z_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC6021039.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAARNLEAASWKDINEI
                     YQRADPTAPLFYLRKKGAIVPKEEYVESFDALGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHISRQVARFAGAHTIVSGYLAFGSDESYKNHWDTRDVYAIQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHLA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQGKHEEVRKLIYQLIELDFLELL"
     gene            2999..3205
                     /locus_tag="ACFM4Z_000984"
     CDS             2999..3205
                     /locus_tag="ACFM4Z_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021040.1"
                     /translation="MKNWKQFTFFVILVIACYQVLYFLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNTNQ"
     gene            3410..3670
                     /locus_tag="ACFM4Z_000985"
     CDS             3410..3670
                     /locus_tag="ACFM4Z_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HFC6021041.1"
                     /translation="MERKNNIIELSIENLELIYGGGLGERILEGGANGAQIGGSIGAG
                     IGLGVGIVSGGIPAGPAIILGGTVGAAVGAVTGMGVAAYEYY"
     gene            3830..4456
                     /locus_tag="ACFM4Z_000986"
     CDS             3830..4456
                     /locus_tag="ACFM4Z_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HFC6021042.1"
                     /translation="MNRPKQPFFRPEVTVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITTKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHENETRSLKATVERLENQKLH
                     ILQQIDGQKRRIRLAEDQWQKRHWADRNCRDWAWYPPIWRRAANLFIL"
     gene            4360..4608
                     /locus_tag="ACFM4Z_000987"
                     /pseudo
     CDS             4360..4608
                     /locus_tag="ACFM4Z_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFP transporter"
     gene            5507..5659
                     /locus_tag="ACFM4Z_000988"
                     /pseudo
     CDS             5507..5659
                     /locus_tag="ACFM4Z_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
     gene            5816..7558
                     /gene="hrpB"
                     /locus_tag="ACFM4Z_000989"
     CDS             5816..7558
                     /gene="hrpB"
                     /locus_tag="ACFM4Z_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HFC6021043.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTVRKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISAFNNKFPLYRNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEGKS
                     DLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLAHK
                     TDLTDATGTETASGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQY
                     QSSLAAERMLWRNRLHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYL
                     NRWQLDGKLSYKRGTGMRQSMPAPEENGGGTIPGTSRMKIITAGLDAAAPFMLGKQQF
                     FYATAIQAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLFGERGFYWQNTLTWYFHPNH
                     QFYLGADYGRVSGESAQYVSGKQLMGAVVGFRGGHKVGGMFAYDLFAGKPLHKPKGFQ
                     TTNTVYGFNLNYSF"
     gene            7667..>11742
                     /gene="hrpA"
                     /locus_tag="ACFM4Z_000990"
     CDS             7667..>11742
                     /gene="hrpA"
                     /locus_tag="ACFM4Z_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion exoprotein HrpA"
                     /protein_id="HFC6021044.1"
                     /translation="MNKGLHRIIFSKKHSTMVAVAETANSQGKGKQAGSSVSVSLKTS
                     GDLCGKLKTTLKTLVCSLVSLSMVLPAHAQITTDKSAPKNQQVVILKTNTGAPLVNIQ
                     TPNGRGLSHNRYTQFDVDNKGAVLNNDRNNNPFVVKGSAQLILNEVRGTASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGAPQIGKDGALTGFDVRQGTLTVGA
                     AGWNDKGEADYTGVLARAVALQGKLQGKNLAVSTGPQKVDYASGEISAGTAAGTKPTI
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADG
                     TEASPTYLSIETTEKGAAGTFISNDGRIESKGLLVIDTGEDIGLRNGAVVQNNGNRPA
                     ATVLNAGRNLVIESKANVNNAKGSTNLSTGGRTVIKEASIQTGTTVYSSSKGNAELGN
                     NTRITGADVTVLSNGTISSSAVIDAKDTAHIEAGKPLSLEASTVTSDIRLNGGSIKGG
                     KQLALLADDNITAKTTNLNTPGNLYVHTGKDLNLNVDKDLSAASIHLKSDNAAHITGT
                     SKTLTASKDMGVEAGSLNVTNTNLRTNSGNLHIQAAKGNIQLRNTKLNAAKALETTAL
                     QGNIVSDGLHAVSADGHVSLLANGNADFTGHNTLTAKADVNAGSVGKGRLKADNTNIT
                     SSSGDITLVAGNGIQLGDGKQRNSINGKHISIKNNGGNADLKNLNVHAKSGALNIHSD
                     RALSIENTKLESTHNTHLNAQHERVTLNQVDAYAHRHLSITGSQIWQNDKLPSANKLV
                     ANGVLALNARYSQIADNTTLRAGAINLTAGTALVKRGNINWSTVSTKTLEDNAELKPL
                     AGRLNIEAGSGTLTIEPANRISAHTDLSIKTGGKLLLSVKGGNAGAPSAQVSSLEAKG
                     NIRLVTGETDLRGSKITAGKNLVVATTKGKLNIEAVNNSFSNYFPTQKAAELNQKSKE
                     LEQQIAQLKKSSPKSKLIPTLQEERDRLAFYIQAINKEVKGKKPKGKEYLQAKLSAQN
                     IDLISAQGIEISGSDITASKKLNLHAAGVLPKAADSEAAAILIDGITDQYEIGKPTYK
                     SHYDKAALNKPSRLTGRTGVSIHAAAALDDARIIIGASEIKAPSGSIDIKAHSDIVLE
                     AGQNDAYTFLKTKGKSGKIIRKTKFTSTRDHLIMPAPVELTANGITLQAGGNIEANTT
                     RFNAPAGKVTLVAGEELQLLAEEGIHKHELDVQKSRRFIGIKVGKSNYSKNELNETKL
                     PVRVVAQTAATRSGWDTVLEGTEFKTTLAGADIQAGVGEKARVDAKIILKGIVNRIQS
                     EEKLETNSTVWQKQAGRGSTIETLKLPSFESPTPPKLSA"
BASE COUNT         3329 a         2721 c         2804 g         2888 t
ORIGIN      
        1 attcccgctt ttgcgggaat gacgggattt tagatttttg ttttcgcgga ataatgagcc
       61 aaaagccgtc tgaacctaaa aaaccgccac tttcagatgg catcaaaaaa ccgcaggcac
      121 gatacctgcg gtttgccgtt tgcgtttcag gggagcaggg ggattgcgtc caagccgtgt
      181 gtttccttca gtccgaacat gatgttcata ttttgcaccg cctgacccgc cgcgcctttg
      241 acgaggttgt cgatgacgga aaggacgacc cacacatcgg attgcgccgc ctgttggatg
      301 ctgatgcggc agaggtttgc gccgcgcacg ctgcgggttt cgggtgtcga accggcgggc
      361 aggatgtcca cgaacaggct gtcgcggtaa tagtcgcgca ggatggtttc ggggcagatg
      421 ccgtctgaaa ggtggaggta aacggtggcg tgcataccgc gtatcatcgg cgcgaggtgc
      481 ggcgtgaaca cgaatccttc ggcgatgccg tcctgaagcc cggcgatggt ctgcctgatt
      541 tcgggcaggt ggcggtgtcc agctatgccg taggctttga agttgtcgcc gacttcgcac
      601 aacagcgaac cgacattgcc tttcctgccc gcgccggaca cgccggattt gcagtcggca
      661 atcagcggca taccgggctt cagacggcat tgccgcaaca gcggcacgag cggcagggat
      721 acgcaggtcg ggtagcagcc ggggttggcg acgaggcgtg cctgtgcgac ggcttcgcgg
      781 ttgagttcgc tcaatccgta cacggcttgg gaaacgaggt cgggggcggc gtgggtcatg
      841 ccgtaccagt gttcccaggt cggaatgtcc cgtatgcgga agtcggcgga aaggtcgatg
      901 acgcgcacgc cctgttcaat caggcgcggc gcgtctttca tggcgatgcc gttgggcgtg
      961 gcgaagaaga cgatgtcgca ttgttccaaa cctgcctcgt cgggcgtttg gaaggcgagg
     1021 ccgtacacgc cgcgcaaact cggaaagtaa tcggcaactg cagttcccgc ttcgctgcgg
     1081 ctggttacgg cggcgacttc gacatcggga tgggcggcaa gcaggcgcag cagttccacg
     1141 cccgtgtagc ccgtcgcgcc gacaatgccg actttgattt ttttactcat ggtttttcct
     1201 ttgtgtggtt ggcgggtatg ccgtccgaac gctgtccgac ggggttttga ttggcggtgg
     1261 aaggacggta cacaaagcgt ttgaaaagcg tgtctaattt aatggcatcc gcctctgttt
     1321 ttaaaaccca gccctgccgt ccggaataaa cataaccgta ggcgcgaaag tcggcaggat
     1381 atttcggtgc ctgccccaaa cccaaaccgt atgcggcgaa agcggtggtc gtggagaagg
     1441 aaagcaggag gaggacgagg gttttcataa ggttccaaac cggacggggc gatgaagccc
     1501 gattatagca aaaggtcgtc tgaaaaccgt tttcagacga ccttttattt aattacaata
     1561 aaaatgttaa caacaaaaaa caaaccgctt tttttcgttt ggaaacaaaa caaattttta
     1621 tagtggatta aatttaaacc agtacagcgt tgcctcgcct tgccttgccg tactatctgt
     1681 actgtctgcg gtttcgtcgc cttttcctga tttttgttaa tccactataa ctaaaattgt
     1741 caacaattcc ttgacgcaca cacaaaaaaa actttaatat ttgtaaatat atgtaacatt
     1801 ataaatttta aatattttct gagtaagggg aagtaatgag catccatctc gacttcggca
     1861 ttagtcctaa aacgttccga cagacttatc tgtatcaaaa gcccaagctc tttaaaggag
     1921 cggctcggaa tctcgaagcc gcatcttgga aagatatcaa cgagatatac caacgagcag
     1981 acccaaccgc accgctgttt tatctgcgta aaaaaggtgc aattgttcct aaagaagaat
     2041 acgtcgaaag tttcgacgct ttgggcaaaa ctcgctaccg ttttattaaa tccgttatct
     2101 acgaacatat gaagaatggt gcgtcgttag tctataacca tattaacaac gagccgtttt
     2161 cagatcatat ttcccgtcaa gtcgcccgct ttgctggcgc acatactatt gtcagtggat
     2221 atcttgcttt tggcagcgac gaatcttata aaaaccattg ggacacccgc gatgtgtatg
     2281 ccatccagct tttcggcaag aaacgttggc aacttactgc ccctgatttc cctatgccat
     2341 tgtatatgca acagactaaa gatactgata tttccattcc tgaacatatc gatatggata
     2401 ttatccttga agcaggtgat gtcctctaca tcccgcgcgg ttggtggcac agacctatcc
     2461 cgctcggctg tgaaaccttc cacctcgctg tcggtacctt tccgcccaac ggctataatt
     2521 acctcgagtg gctaatgaag aaattcccca cgatagaaag tctgcgccac agtttctcag
     2581 actgggagca agataggacg cgtatcaacg atactgccgc acaaattgct gccatgattg
     2641 ccgaccccgt caattacgaa gccttcagtg aagacttcct cggcaaagaa cgcaccgata
     2701 ccgcttttca tctcgaacag ttcgcgaatc ccaacgctac tccgctttca gacgacgtca
     2761 ggttgagact aaatgccaat aatttggata cgttggaaaa ggggtatttg attgggaatg
     2821 ggatgaagat aagcgtagat gaattgggga aaaaagtttt agaacacatc ggtaagaatg
     2881 aaccgttatt gttgaaaaat ctactggtta acttcaatca gggaaaacat gaagaagtta
     2941 ggaagttgat ttatcagttg atagagttag attttctgga acttttgtga gggattctat
     3001 gaaaaactgg aagcaattta catttttcgt aatattagta atagcttgtt atcaagtgct
     3061 atatttttta tcagatatgt ttcttctcga ctacattaac aaatatagtt ggaatttgaa
     3121 ttttattcag ggtactttga attttttttc aatatatctg ccatatgttt ttgttagccg
     3181 aatttttagg aatactaacc aatgaaagac taggctatga aatcaaaatt tatgtataag
     3241 ttactggtac ttgcaaagta cctgtatatt ttaatttgta tatcaatacc aatcgttttt
     3301 atttatgaaa gctaccaagg agatgatgtt gattttatga tttatattca tgcttttatt
     3361 attttttcca tattgggtgt attgactcaa tattctacgt ctaatttttt tggaaaggaa
     3421 aaataacatc attgaactat ccattgagaa tctggagcta atttatggcg gaggcttagg
     3481 ggagagaatc cttgaaggag gagcaaatgg tgcacaaata ggaggatcaa ttggtgcagg
     3541 tattggtctg ggtgtcggta tagtttctgg tggtattcct gccggaccgg ctattattct
     3601 cggtggcaca gttggtgctg ctgttggtgc tgtaactggt atgggtgttg cagcatatga
     3661 gtattactaa gaaactcagc ccaaagggac agtaacggtc ggtccgattt ctgcttttgc
     3721 tgtggacaaa tcaggaaacg gctacactga cggtacagat tactgctgat atttctcatc
     3781 tttcagacga tctctagggg tcgtctgaaa ctttcttaac ttcaatttta tgaatagacc
     3841 caagcaacct ttcttccgcc ctgaagtcac agttgcccgg caaaccagcc tgacgggtaa
     3901 agtgattctg acacgaccgt tgtcattttc cctatggacg acatttgcat cgatatctgc
     3961 gttactgatt atcctgtttt tgatatttgg taactatacg cgaaagacaa cagtggaggg
     4021 acaaatttta cctgcatcgg gcgtaatcag ggtgtatgca ccggatacgg ggacaattac
     4081 aacgaaattc gtggaagatg gagaaaaggt caaggctggc gacaagctat ttgcgctttc
     4141 gacctcacgt ttcggcgcag gaggtagcgt gcagcagcag ttgaaaacgg aggcagtttt
     4201 gaagaaaacg ttggcagaac aggaactggg tcgtctgaag ctgatacacg agaatgaaac
     4261 gcgcagcctt aaagcaactg tcgaacgttt ggaaaaccag aaactccata ttttgcaaca
     4321 gatagacggt cagaaaaggc gcattagact tgcggaagat cagtggcaaa aacggcactg
     4381 ggcagacagg aattgtcggg attgggcatg gtatcctccg atttggcgaa gagcagcgaa
     4441 cctgtttatc ttgtgaaaat aaaacccgac aaatcaacca tcactgcata cggtgaggaa
     4501 aaaccgctgc aaatcggcat gacgttggaa gcagacatcc tgcacgagaa acggcgattg
     4561 tacgaatggg tattggagct gatttatagt atgtcgggca aactgtaaga cttgaggctg
     4621 aaagataaag aagttgtctg aaaccttttc agacgaccag aagacaatat ataaaaacac
     4681 aaacttcaaa actgcctgaa accatcccct tttacaggca ctccgtctgt aaaagcaagg
     4741 atagggcatc tgcccttttg attgtttgat taacgataca aggagtttca aaatgagagt
     4801 tttatagtgg attaacaaaa accagtacag cgttgcctcg ccttgccgta ccatttgtac
     4861 tgtctgcggc ttcgtcgcct tgtcctgatt taaatttaat ccactatatg tgttcatgaa
     4921 atgacttggg tcggaggctc aggtaatgcg taacaaagct catattattg cgaaatttgc
     4981 gaatctgcag ggcttaacga tacgggaaat cctgataaat ctttaggatt gccaaacaat
     5041 acgttcagta atccgcctgg ttggggagct acaatcggag ctttagcagg tagccgcata
     5101 gggatgcctg aatttggtac gtttgcgagc catgccattg aaaatttcga ctggtcatgg
     5161 tatcgacgtt atagggaaat tgccgaaacg attgaacgag aatattcagg cggtttgcct
     5221 taatagttga ggaggtcatg atgtttgcca aacattatca attcatcgca ctcggcatca
     5281 tgctgcttct ttatatgttg attctctata cgaccgattt ttccaatctg acgtattgga
     5341 tgctgttttt tatctgtttt attacaggaa aaatattagc tcgtttgtta gagaaaagct
     5401 ttaaataaaa tagcagctag tcgcaaaagg tcgtctgaaa ccttttcagg cggcctttct
     5461 aaaatacatc caacttccta atccctattt ttcaaaaagg aaatctatgc cccatctgca
     5521 aaacctgtct ttgggcttaa agaaaaagct gcctgttatc ctgcaaacag aaatatcaga
     5581 atgcggcttg gcatgtctgg cggctgtggc gggatttcat ggtttccata cggatttacg
     5641 cgcactgcgt tcaaaatact gtccgagacc tttgcaaaat tccccaaaat cccctaaatg
     5701 tcttggtggg aattttgggg attttgggga attttgcaaa ggtctcattc tataactgta
     5761 aatactttta aatttatgac agaatagtaa atattgctaa aataatatta atgtcatgaa
     5821 attttttcct gctccatgtt tgttggttat cctggctgtc atacccctta aaaccttagc
     5881 tgccgatgaa aacgatgcag aacttatccg ttccatgcag cgtcagcagc acatagatgc
     5941 tgaattgtta actgatgcaa atgtccgttt cgagcaacca ttggagaaga acaattatgt
     6001 cctgagtgaa gatgaaacac cgtgtactcg ggtaaattac attagtttag atgataagac
     6061 ggtgcgcaaa ttttcttttc ttccttctgt gctcatgaaa gaaacagctt ttaaaactgg
     6121 gatgtgttta ggttccaata atttgagcag gctacaaaaa gccgcgcaac agatactgat
     6181 tgtgcgtggc tacctcactt cccaagctat tatccaacca cagaatatgg attcgggaat
     6241 tctgaaatta cgggtatcag caggcgaaat aggggatatc cgctatgaag aaaaacggga
     6301 tgggaagtct gccgaaggca gtattagtgc attcaataac aaatttccct tatataggaa
     6361 caaaattctc aatcttcgcg atgtagagca gggcttggaa aacctgcgtc gtttgtcgag
     6421 tgttaaaaca gatattcaga ttataccgtc cgaagaagaa ggcaaaagcg atttacagat
     6481 caaatggcag cagaataaac ccatacggtt cagtatcggt atagatgatg cgggcggcaa
     6541 aacgaccggc aaatatcaag gaaatgtcgc tttatcgttc gataaccctt tgggcttaag
     6601 cgatttgttt tatgtttcat atggacgcgg tttggcgcac aaaacggact tgactgatgc
     6661 caccggtacg gaaactgcaa gcggatccag aagttacagc gtgcattatt cggtgcccgt
     6721 aaaaaaatgg ctgttttctt ttaatcacaa tggacatcgt taccacgaag caaccgaagg
     6781 ctattccgtc aattacgatt acaacggcaa acaatatcag agcagcttgg ccgccgagcg
     6841 catgctttgg cgtaacagac ttcataaaac ttcagtcgga atgaaattat ggacacgcca
     6901 aacctataaa tacatcgacg atgccgaaat cgaagtgcaa cgccgccgct ctgcaggctg
     6961 ggaagccgaa ttgcgccacc gtgcttacct caaccgttgg cagcttgacg gcaagttgtc
     7021 ttacaaacgc gggaccggta tgcgccaaag tatgcccgca cctgaagaaa acggcggcgg
     7081 tactattcca ggcacatccc gtatgaaaat cataaccgcc ggattggatg cagcggcccc
     7141 gtttatgttg ggcaaacagc agtttttcta cgcaaccgcc attcaagctc aatggaacaa
     7201 aacgccgttg gttgcccaag ataaattgtc aatcggcagc cgctacaccg ttcgcggatt
     7261 tgatggggag cagagtcttt tcggagagcg aggtttctac tggcagaata ctttaacttg
     7321 gtattttcat ccgaaccatc agttctatct cggtgcggac tatggccgcg tatctggcga
     7381 aagtgcacaa tatgtatcgg gcaagcagct gatgggtgca gtggtcggct tcagaggagg
     7441 gcataaagta ggcggtatgt ttgcttatga tctgtttgcc ggcaagccgc ttcataaacc
     7501 caaaggcttt cagacgacca acaccgttta cggcttcaac ttgaattaca gtttctaacc
     7561 tctgaatttt ttactgatat ttagacggtc tttccttatc ctcagaccgt caaactttac
     7621 ctacgtactt ggcgcgcagt acgttcatct tcaaaatgga atagacatga ataaaggttt
     7681 acatcgcatt atctttagta aaaagcacag caccatggtt gcagtagccg aaactgccaa
     7741 cagccagggc aaaggtaaac aggcaggcag ttcggtttct gtttcactga aaacttcagg
     7801 cgacctttgc ggcaaactca aaaccaccct taaaactttg gtctgctctt tggtttccct
     7861 gagtatggta ttgcctgccc atgcccaaat taccaccgac aaatcagcac ctaaaaacca
     7921 gcaggtcgtt atccttaaaa ccaacactgg tgcccccttg gtgaatatcc aaactccgaa
     7981 tggacgcgga ttgagccaca accgctatac gcagtttgat gttgacaaca aaggggcagt
     8041 gttaaacaac gaccgtaaca ataatccgtt tgtggtcaaa ggcagtgcgc aattgatttt
     8101 gaacgaggta cgcggtacgg ctagcaaact caacggcatc gttaccgtag gcggccaaaa
     8161 ggccgacgtg attattgcca accccaacgg cattaccgtt aatggcggcg gctttaaaaa
     8221 tgtcggtcgg ggcatcttaa ctaccggtgc gccccaaatc ggcaaagacg gtgcactgac
     8281 aggatttgat gtgcgtcaag gcacattgac cgtaggagca gcaggttgga atgataaagg
     8341 cgaagccgac tacaccgggg tacttgctcg tgcagttgct ttgcagggga aattacaggg
     8401 taaaaacctg gcggtttcta ccggtcctca gaaagtagat tacgccagcg gcgaaatcag
     8461 tgcaggtacg gcagcgggta cgaaaccgac tattgccctt gatactgccg cactgggcgg
     8521 tatgtacgcc gacagcatca cactgattgc caatgaaaaa ggcgtaggcg tcaaaaatgc
     8581 cggcacactc gaagcggcca agcaattgat tgtgacttcg tcaggccgca ttgaaaacag
     8641 cggccgcatc gccaccactg ccgacggcac cgaagcttca ccgacttatc tctccatcga
     8701 aaccaccgaa aaaggagcgg caggcacatt tatctccaat gatggtcgga tcgagagcaa
     8761 aggcttattg gttattgata ccggggagga catcggtttg cgtaacggag cggtcgttca
     8821 aaacaacggc aaccgccctg ctgctacggt attaaatgcc ggccgtaatt tggttatcga
     8881 aagcaaagca aatgtgaaca atgctaaagg ttcgaccaat ctgtcgaccg gcggccgtac
     8941 cgtcatcaag gaggccagta ttcagactgg cactaccgta tacagttcca gcaaaggcaa
     9001 cgccgaatta ggcaataaca cacgcattac cggggcagat gttaccgtat tatccaacgg
     9061 caccatcagc agttccgccg taatagatgc caaagacacc gcacacatcg aagcaggcaa
     9121 accgctttct ttggaagctt caacagttac ctccgatatc cgcttaaacg gaggcagtat
     9181 caagggcggc aagcagcttg ctttactggc agacgataac attactgcca aaactaccaa
     9241 tctgaatact cccggcaatc tgtatgttca tacaggtaaa gatctgaatt tgaatgttga
     9301 taaagatttg tctgccgcca gcatccattt gaaatcggat aacgctgccc atattaccgg
     9361 caccagtaaa accctcactg cctcaaaaga catgggtgtg gaggcaggct cgctgaatgt
     9421 taccaatacc aatctgcgta ccaactcggg taatctgcac attcaggcag ccaaaggcaa
     9481 tattcagctt cgcaatacca agctgaacgc agccaaggct ctcgaaacca ccgcattgca
     9541 gggcaatatc gtttcagacg gccttcatgc tgtttctgca gacggtcatg tatccttatt
     9601 ggccaacggt aatgccgact ttaccggtca caataccctg acagccaagg ccgatgtcaa
     9661 tgcaggatcg gttggtaaag gccgtctgaa agcagacaat accaatatca cttcatcttc
     9721 aggagatatt acgttggttg ccggcaacgg tattcagctt ggtgacggaa aacaacgcaa
     9781 ttcaatcaac ggaaaacaca tcagcatcaa aaacaacggt ggtaatgccg acttaaaaaa
     9841 ccttaacgtc catgccaaaa gcggggcatt gaacattcat tccgaccggg cattgagcat
     9901 agaaaatacc aagctggagt ctacccataa tacgcatctt aatgcacaac acgagcgggt
     9961 aacgctcaac caagtagatg cctacgcaca ccgtcatcta agcattaccg gcagccagat
    10021 ttggcaaaac gacaaactgc cttctgccaa caagctggtg gctaacggtg tattggcact
    10081 caatgcgcgc tattcccaaa ttgccgacaa caccacgctg agagcgggtg caatcaacct
    10141 tactgccggt accgccctag tcaagcgcgg caacatcaat tggagtaccg tttcgaccaa
    10201 aactttggaa gataatgccg aattaaaacc attggccgga cggctgaata ttgaagcagg
    10261 tagcggcaca ttaaccatcg aacctgccaa ccgcatcagt gcgcataccg acctgagcat
    10321 caaaacaggc ggaaaattgc tgttgtctgt aaaaggagga aatgcaggtg cgcctagtgc
    10381 tcaagtttcc tcattggaag caaaaggcaa tatccgtctg gttacaggag aaacagattt
    10441 aagaggttct aaaattacag ccggtaaaaa cttggttgtc gccaccacca aaggcaagtt
    10501 gaatatcgaa gccgtaaaca actcattcag caattatttt cctacacaaa aagcggctga
    10561 actcaaccaa aaatccaaag aattggaaca gcagattgcg cagttgaaaa aaagctcgcc
    10621 taaaagcaag ctgattccaa ccctgcaaga agaacgcgac cgtctcgctt tctatattca
    10681 agccatcaac aaggaagtta aaggtaaaaa acccaaaggc aaagaatatc tgcaagccaa
    10741 gctttctgca caaaatattg acttgatttc cgcacaaggc atcgaaatca gcggttccga
    10801 tattaccgct tccaaaaaac tgaaccttca cgccgcaggc gtattgccaa aggcagcaga
    10861 ttcagaggcg gctgctattc tgattgacgg cataaccgac caatatgaaa ttggcaagcc
    10921 cacctacaag agtcactacg acaaagctgc tctgaacaag ccttcacgtt tgaccggacg
    10981 tacgggggta agtattcatg cagctgcggc actcgatgat gcacgtatta ttatcggtgc
    11041 atccgaaatc aaagctccct caggcagcat agacatcaaa gcccatagtg atattgtact
    11101 ggaggctgga caaaacgatg cctatacctt cttaaaaacc aaaggtaaaa gcggcaaaat
    11161 catcagaaaa accaagttta ccagcacccg cgaccacctg attatgccag cccccgtcga
    11221 gctgaccgcc aacggtatca cgcttcaggc aggcggcaac atcgaagcta ataccacccg
    11281 cttcaatgcc cctgcaggta aagttaccct ggttgcgggt gaagagctgc aactgctggc
    11341 agaagaaggc atccacaagc acgagttgga tgtccaaaaa agccgccgct ttatcggcat
    11401 caaggtaggt aagagcaatt acagtaaaaa cgaactgaac gaaaccaaat tgcctgtccg
    11461 cgtcgtcgcc caaactgcag ccacccgttc aggctgggat accgtgctcg aaggtaccga
    11521 attcaaaacc acgctggccg gtgccgacat tcaggcaggt gtaggcgaaa aagcccgtgt
    11581 cgatgcgaaa attatcctca aaggcattgt gaaccgtatc cagtcggaag aaaaattaga
    11641 aaccaactca accgtatggc agaaacaggc cggacgcggc agcactatcg aaacgctaaa
    11701 actgcccagc ttcgaaagcc ctactccgcc caaattgtcc gc
//
LOCUS       DAXHNE010000047        11707 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.047, whole genome shotgun sequence.
ACCESSION   DAXHNE010000047 DAXHNE010000000
VERSION     DAXHNE010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11707
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.047"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(231..1277)
                     /locus_tag="ACFM4Z_000991"
     CDS             complement(231..1277)
                     /locus_tag="ACFM4Z_000991"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6021045.1"
                     /translation="MMKIKALMIAAALLAAADVHAAPQKAKTASAKVAKVAKVAKVAK
                     VAKVAKVAATAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKANLPKE
                     AQISESELSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNSALKNLSPAQRRD
                     FADNIIAEKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILIKA
                     DSENAAVGAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVMVPAFE
                     EAVHALKPGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAEQATVN
                     LLRDLHSGAYVDIR"
     gene            complement(1379..3787)
                     /locus_tag="ACFM4Z_000992"
     CDS             complement(1379..3787)
                     /locus_tag="ACFM4Z_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HFC6021046.1"
                     /translation="MARLFSLKPLVLALGLCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVRGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDVGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPSVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            3857..4861
                     /gene="amgK"
                     /locus_tag="ACFM4Z_000993"
     CDS             3857..4861
                     /gene="amgK"
                     /locus_tag="ACFM4Z_000993"
                     /EC_number="2.7.1.221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate/N-acetylglucosamine kinase
                     AmgK"
                     /protein_id="HFC6021047.1"
                     /translation="MQRQIKLKNWLQTVYPERDFDLTFAAADADFRRYFRATFSDGSS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLHRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            complement(4918..5616)
                     /locus_tag="ACFM4Z_000994"
     CDS             complement(4918..5616)
                     /locus_tag="ACFM4Z_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC6021048.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            5834..7372
                     /gene="murJ"
                     /locus_tag="ACFM4Z_000995"
     CDS             5834..7372
                     /gene="murJ"
                     /locus_tag="ACFM4Z_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFC6021049.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEAAEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAQDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFGIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LSFKDAAVNRVMKQMAPAILGVSVAQVSLVINTIFASYLQSGSVSWMYYADRMMELPS
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRRHGIYQPGKGWAAFLAKMLL
                     SLAVMGGGLYAAQIWLPFDWAHAGGMQKAARLFILIAVGGGLYFASLAALGFRPRHFK
                     RVEN"
     gene            7504..8460
                     /locus_tag="ACFM4Z_000996"
     CDS             7504..8460
                     /locus_tag="ACFM4Z_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFC6021050.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRPAPTYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSRSHYAVELMLAVRSRGWHGAVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGERGENALPQAAA
                     VISAPVDAEAAGRRFDSGITRLLYTRYFLRTLIPKARSLHGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPTPDEVSEAV
                     TLFQPAHGGHAGFVGSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            8511..9293
                     /gene="trpC"
                     /locus_tag="ACFM4Z_000997"
     CDS             8511..9293
                     /gene="trpC"
                     /locus_tag="ACFM4Z_000997"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HFC6021051.1"
                     /translation="MTDILNKILTAKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIGS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQEQLERFEAVA
                     HELGMTVLLELHNETELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMQSRGVHTFLIGETFMRADDIEAEVGKLF"
     gene            9358..11658
                     /gene="recQ"
                     /locus_tag="ACFM4Z_000998"
     CDS             9358..11658
                     /gene="recQ"
                     /locus_tag="ACFM4Z_000998"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFC6021052.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVANLHAAGIEAAAVNSGTSADEAREIAD
                     RLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGM
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLDFIRKEMTGQSGIVYCLSRKKVEDVAQFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLAVCETAACRRVLLLKHFGEASEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNILRGKSDDWIRGNRHEQLS
                     TFGIGTELSDKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QNLNGLHDIYGLGEAKTERFGHGILEVCRNAAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLAATPETETDLEGVHGLGSVRAAKYGWDILAVCRP
                     FSDGIDETAKHKRRLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLGQNAV"
BASE COUNT         2368 a         3297 c         3370 g         2672 t
ORIGIN      
        1 ttttttgttt ttccgataaa ttaccacaac ccaaaatccc gtcattcccg cgacggcggg
       61 aatccagtcc tttaaactcc agccattccc gataaattcc tgttactttt cgtttctaga
      121 ttcccgcttt cgcgggaatg atgaatgacg gtgtaaaagt aactcgaaat ccaaaaagcc
      181 ataccgcccg gatttttgcc gatcggtatg gctttttgct tcaaaccgcc ttagcggatg
      241 tcgacatacg cgccggaatg caggtcacgc aacaggttga cggttgcctg ttcggctttt
      301 tgttggaaga tgtattgccg cacggaattg cggatacgtt cctgaggtgt gccggcatcg
      361 cgcacttcgt tcaatttgat gatatgccag ccgaattggg tgcggacggg cgcgccgacc
      421 tgtccgggtt tgagcgcgtg gacggcttct tcaaaggcgg gaaccatcac gccgtcggca
      481 aaccagccca aatctccgcc gttgcccgcg ctcgcgtctt gcgaatattg gcgcgctagg
      541 ctggaaaagt ctgtgccgct gcgggcctct ccgtagattt tgcggatggt gctttccgcg
      601 ccgacggcgg cgttttcgct gtcggcttta atcaggatgt gttgggcgcg gtattggcgc
      661 aacggtgcgc cttcgggcag ggtgatgcct tgtttttgcg cctgctcgag gaaggcatcg
      721 atttcagctt cgctcacgcg gctgttctgc atcactgcct gctggcggac tttttcggca
      781 atgatgttgt cggcaaaatc gcggcgttgg gcggggctga ggtttttgag ggcggaattt
      841 tttgcgacga cggcatcgat ttccgcttcg cttgcttgaa tgttgcggcg tttgcccgcc
      901 tgtacaatca gggattggtt gacaagctgc atcagcacct gtcgggacag ctcggattcg
      961 cttatctgcg cctctttggg caggttggct ttggcttcgg caacggcttc ggcaagccgg
     1021 cggcgcgtga tgacttcgtt gtcggcaacg gcggcaatgc cgtctgaaaa gcggataccg
     1081 ccctgctgtt gtgcgggtgc ggcttctttt tgcgccgtgg cggcaacttt ggcaactttg
     1141 gcaactttgg caactttggc aactttggca actttggcaa ctttggcgga tgcggttttt
     1201 gccttttgcg gtgcggcgtg gacatcggct gctgccagca atgcggcggc aatcatcagg
     1261 gctttgattt tcatcattct ttccttatcg tttgaatcct acctttgatg gcgggtttgg
     1321 ggttgtcggt ggaatgccgt cttgtgcggg aacggtgctc ctgccgggtt tccgacggtc
     1381 agggtcgttt gttgcgtccg gcggaaagag agtgggcggg gatgtagccg ggaacggcga
     1441 catccatcct gtctgcgggg tttctgccga cactgctgag gtctttcaac tgaagtgaga
     1501 aaaagacagc gtttttgtag gtgttttcgc cggtaacgta gcgttgggcg tacacgcccg
     1561 cgccccagca gccgcaactg cttttgtatt ccgcacccgc cagcacctct atcggttttt
     1621 tggcttcaaa accgtagttg taacggacga cggccgacag gttgcgcgtc agcggccatt
     1681 gtgcggacag gtcgagctgg ctgagtttgt cgtaaaaata ggaaccgtcg gacttcaggt
     1741 agattttttc gttgcgcccg tatttgtagc gggcgttcag cactttgccc tgtgcgggac
     1801 ggtagcttgc accgacggcg tagttctcgg cgcgtttgtc gttttggttg tagtggatgc
     1861 tgctgtcgag gatgaagcgg ctgccgatgc tgccggaggc aaatgccacc cagtcggaac
     1921 ggttgcgcgg ttttttgccg acgctgccgt caagcatcac cgcatcatcc ttgaaataga
     1981 atttctgacc gatgccggcg cggaaacgct cttcccccgt cgcgccgtcc aaaatacggc
     2041 tttgcacggc ggcggaaagg ctgtttgcgg tgttaatcct gtcgttgccg taatagaggt
     2101 tttcgcgaaa gagctgcccg tagccgaagc tgctttccga cgaatcgaaa ttgggcaggt
     2161 cgttttggga tttggcagga atatagttgt agaacaggcg cggctcgagg gtttgcagga
     2221 ctccgccgcc gaacatccgc gtattccgct caaaagttgc gccgctgtcg atgttgacaa
     2281 tgggcagagt gcggctgacg cgtcgggctt cttggctgcc gaagcggttg aggctgtaat
     2341 aggtggcgtg cagtccgagt ttgggacgga cgtaacccca gctgttgctg aaatcccatt
     2401 tgatgtcggg atagacgacg aggcggctgc cgtcttggcg gctgtcgtgg ctgaagcggg
     2461 taaattgtgc ggacacgccg atttgcgccc tgccggtgtt tttgcgccaa tcggcggaaa
     2521 ggcgcggcat cagggcatac ggtttgtctt tgtagccgct ttggtttgcc agcgtctggt
     2581 atttcagaac cgaaaggccg gcattcaggc tgccgcccgc cgccctgccg ccataatcca
     2641 gccatacacg gcggttgagg ttgacgttgc cggcgatttc tttgttgccg taaaagtcgc
     2701 ggtagtagcc gctgtcggag acttggttga aatcgacacc cgcctgaagc gtgtcggaaa
     2761 tgtcgtgccg atgctgccat ttcgcctgat agcggttatt cctgccgctt ttcttgtcgt
     2821 gcggcagcca ggtcaggtcg gactggccgg cataatccgg ccgcaggtag cgtacctgcc
     2881 cgtcaaagac cgcgccgcgt tcgccgatca cgctgggcgc gaacgtggca tcgagattgg
     2941 gggcaaggtt gaaataatag ggaacggaaa gggaaacgcc gtccgaaccg gcggacagtg
     3001 agggaacaag caggccgctt ttgcggttgc cgtcaagcgg gaagtccgcc caaggggtgt
     3061 agaaaatggg aacgccgccg aacacgaagg cggcgtgttt ggcaacgcct atgccttttt
     3121 cccgatcggc ttcgacggaa gcggccttga cataccagcc gacatcgccg gcggaacagg
     3181 tgttgaattg ggtttccgtc agtttgtaat gcccttcgcc caacatttcg gcggtgcggc
     3241 tgacgctttg cagccgccgt ccgccgtgtt cggtttccat acggacgttg tgcgcttccc
     3301 cggtctgctg ctcgagattg taggtcaggg tttcgccccg aatcagcgta ccgtcctgtt
     3361 ggagggcgaa ccggtcgcct gcggtaacgg tgtcgcccga ctggtcgtaa tccgcccaat
     3421 cggtattgag ggtcgtccgg ttgcgttcga cgacgacgtt gccttcggca cgcacctgca
     3481 cctgcgactg tccttccacc ttgtcggcaa caatgcgcgt atagtcttcg gggatggatg
     3541 cttcgccgct gcctcggacg gcggcttcgg ttctctcggg gctgccgctt tcgttactgc
     3601 aaaacaggca ggtcgaaccg aggctcaggc tggtaggctg tatgggttcg gacacgctcc
     3661 gaacgcttcc tccggcggtc ggattgtccg tttcctccgc cgcaacggca tcggcggcgg
     3721 cgcaatgcgt gccgaagcag aggcccaatg ccagcaccag tggtttgagt gaaaataaac
     3781 gagccaaaat cgcccctcaa gtcggtttgc cagttagaat agcgtttatt gtaacctgaa
     3841 atgctttcgt actgttatgc aacgacaaat caaactgaaa aattggcttc agaccgttta
     3901 tcccgaacgg gacttcgatc tgacttttgc ggcggcggat gctgatttcc gccgctattt
     3961 ccgtgcaacg ttttcagacg gcagcagtgt cgtctgcatg gatgcaccgc ccgacaagat
     4021 gagtgtcgca ccttatttga aagtgcagaa actgtttgac atggtcaatg tgccgcaggt
     4081 attgcacgcg gacacggatc tggggtttgt ggtattgaac gacttgggca atacgacgtt
     4141 tttgaccgcg atgcttcagg aacagggcga aacggcgcac aaagccctgc ttttggaggc
     4201 aatcggcgag ctggtcgaat tgcagaaggc aagccgtgaa ggggttttgc ccgaatatga
     4261 ccgtgaaacg atgctgcgcg aaatcaacct gttcccggaa tggtttgtcg caaaagaatt
     4321 ggggcgcgaa ttaacattca aacaacgcca actttggcag cagaccgtcg atacgctgct
     4381 gccgcccctg ttggcgcagc ccaaagtcta tgtgcaccgc gactttatcg tccgcaacct
     4441 gatgctgacg cgcggcaggc cgggtgtttt agacttccaa gacgcgcttt acggcccgat
     4501 ttcctacgat ttggtgtcgc tgttgcgcga tgcctttatc gaatgggaag aagaatttgt
     4561 cttggacttg gttatccgct actgggaaaa ggcgcgggct gccggcctgc ccgtccccga
     4621 agcgtttgac gagttttacc gctggttcga atggatgggc gtgcagcggc acttgaaggt
     4681 tgcaggcatc ttcgcacgcc tgtactaccg cgacggcaaa gacaaatacc gtccggaaat
     4741 cccgcgtttc ttaaactatc tgcaccgcgt atcgcgccgt tatgccgaac tcgccccgct
     4801 ctacgcgctc ttggtcgaac tggtcggcga tgaagaactg gaaacgggct ttacgtttta
     4861 aacccaatca aaatgccgtc tgaaaaccaa gtttcagacg gcatttttca aacgggctta
     4921 ctgcgcggct ttttgttctt cgcgtacttt gtccgccaaa aggtcgatgg tgttcatacc
     4981 ggactcccag tcggcaaatt cgactttata tttaccgccg acgataaccg tgggcgtacc
     5041 gtcgatttgg aaggtttcgg tcagctcctg cattttgtcg gcgcgcgcct ggctttcggg
     5101 agattcgtaa gcggcaagga cttttttgcc gtcaaaggcg gtttgttcgc ccagccattt
     5161 tttgaggact tccggctctt gcagcttaat tttctgatta accatcgcat cgaaaatatg
     5221 gctgttcgcc acatctttgc tgtcggcggc agccatatcg acggcggcgg cgaggcgtgc
     5281 cagcgtcagc atttctttct gccagacgac gtgttcggta cgcaggtaca tatcgtcttt
     5341 aaaagacttg gcgtgtttgc ttaaaacagg ttcgaggtgg gcgcagtgcg gacagaaata
     5401 gccgaaaaac tcaaggactt cgactttgcc tgcctgctgt tggggaatcg ggttggcaag
     5461 gacggtatag ttttgccctt cgaccagccc tgccggggcg gcggctgccg aagcggcagg
     5521 cgcgctgtcg gcggggacgc tggtttggac tttgctgtcg cacgcggcaa gggcgaacag
     5581 ggcggcaacg ccgagggcaa ggtgtctgga tttcatacgg ttctccgtga tgttggaaac
     5641 agatgttgga aatcaatcgg aatccggcta ttttattgca tttccgcgta ttgatacagt
     5701 ttgccgccgg aaaaggacgt tttcgtttca ggaaaccgct tcagacggca tcaaacccga
     5761 tgccgtctga agcggtttct gtcgtacaat acgcgccgtt gccccagacg ggtacgactg
     5821 ttgaggaaca atgatgaata tgctgggagc tttggcaaaa gtcggcagcc tgacgatggt
     5881 gtcgcgcgtt ttgggatttg tgcgcgatac ggtcattgcg cgggcattcg gcgcgggtat
     5941 ggcgacggat gcgttttttg tcgcgttcaa actgcccaac ctgcttcgcc gcgtgtttgc
     6001 ggagggggcg tttgcccaag cgtttgtgcc gattttggcg gaatacaagg aaacgcgttc
     6061 aaaagaggcg gcggaggctt ttatccgcca tgtggcgggg atgctgtcgt ttgtactggt
     6121 tatcgttacc gcgctgggca tacttgccgc gccttgggtg atttatgttt ccgcacccgg
     6181 ttttgcccaa gatgccgaca aatttcagct ctccatcgat ttgctgcgga ttacgtttcc
     6241 ttatatatta ttgatttccc tgtcttcatt tgtcggctcg gtactcaatt cttatcataa
     6301 gttcggcatt ccggcgttta cgcccacgtt tctgaacgtg tcgtttatcg tattcgcgct
     6361 gtttttcgtg ccgtatttcg atccgcccgt taccgcgctg gcgtgggcgg tctttgtcgg
     6421 cggcattttg caactcggct tccaactgcc ctggctggcg aaactgggct ttttgaaact
     6481 gcccaaactg agtttcaaag atgcggcggt caaccgcgtg atgaaacaga tggcgcctgc
     6541 gattttgggc gtgagcgtgg cgcaggtttc tttggtgatc aacacgattt tcgcgtctta
     6601 tctgcaatcg ggcagcgttt catggatgta ttacgccgac cgcatgatgg agctgcccag
     6661 cggcgtgctg ggggcggcac tcggtacgat tttgctgccg actttgtcca aacactcggc
     6721 aaaccaagat acggaacagt tttccgccct gctcgactgg ggtttgcgcc tgtgcatgct
     6781 gctgacgctg ccggcggcgg tcggactggc ggtgttgtcg ttcccgctgg tggcgacgct
     6841 gtttatgtac cgcgaattta cgctgtttga cgcgcagatg acgcaacacg cgctgattgc
     6901 ctattctttc ggtttaatcg gcttaatcat gattaaagtg ttggcacccg gcttctatgc
     6961 gcggcaaaac atcaaaacgc ccgtcaaaat cgccatcttc acgctcatct gcacgcagtt
     7021 gatgaacctt gcctttatcg gcccactgaa acacgtcgga ctttcgcttg ccatcggtct
     7081 gggcgcgtgt atcaatgccg gattgttgtt ttacctgttg cgcagacacg gtatttacca
     7141 acctggcaag ggttgggcag cgttcttagc aaaaatgctg ctctcgctcg ccgtgatggg
     7201 aggcggcctg tatgccgccc aaatctggct gccgttcgac tgggcacacg ccggtggaat
     7261 gcaaaaggcc gcccggctct tcatcctgat tgccgtcggc ggcggactgt atttcgcatc
     7321 actggcggct ttgggcttcc gtccgcgcca tttcaaacgc gtggaaaact gaccgatgcc
     7381 gatatccttt ttcggcaaac gcttttccac gcctatattc gggcgcgcat cccccacgtc
     7441 cgcaccagcc ggacgggctg ccgcatcatc aatgccgtct gaaaacaaga aaaccgatac
     7501 cttatgattt taacaccgcc ggacacgccc tttttcctcc gcaacggcaa tgccgacacg
     7561 attgccgcca aattcctgca acgccccgca cccacatacc gccgcgagct gcttcccgac
     7621 agcacgggta aaaccaaagt cgcctacgat ttttcagacg gcatttcgcc cgatgcgccg
     7681 ctggtcgtgc tgtttcacgg tttggaagga agcagccgca gccattacgc ggtcgaactg
     7741 atgctcgcgg tacgcagccg cggttggcac ggcgcagtcg tccatttccg cagctgcggc
     7801 ggcattgcca acaccgctcc ggtgttctac cacttgggcg ataccgccga aatcgccttt
     7861 actttggaca cgcttgccgc gcgttaccgt gaaatatacg ccgtcggcgt atcgctgggc
     7921 ggcaacgcgc tggcaaaata tttgggcgaa cggggcgaaa acgcgctgcc gcaagccgct
     7981 gccgtcatct ccgcacccgt cgatgcagag gcggcaggca gacgcttcga cagcggcatc
     8041 acgcggctgc tctacacgcg ctacttcctc cgcaccctga tacccaaagc acggtcgctc
     8101 cacggttttc agacggcatt tgccgcaggg tgcaaaacac tgggcgagtt tgacgaccgc
     8161 ttcaccgcac cgctgcacgg ctttgccgac cggcacgact actaccgcca aacttcctgc
     8221 aaaccgctgc tcaaacacgt tgccaaaccg ctgctcctgc tcaatgccgc caacgacccc
     8281 ttcctgccgc ccgaagcgct gccgacaccg gacgaagtgt ccgaagccgt taccctgttc
     8341 cagccggcac acggcggaca cgccggcttt gtcggcagca ccggcggcag gctgcacctg
     8401 caatggctgc cgcagaccgt cctgtcctat ttcgacagct tccgcacaaa caggcggtaa
     8461 cggtttgatg ctaatattcc ccctttcccc ggacaaatac ggaacacgac atgaccgaca
     8521 tcctcaataa aatccttacc gccaaggcac aggaagttgc cgctcaaaaa gccgccgtca
     8581 acgccgaaca catccgcgca cttgccgcag aagccgcgcc cgtccgcagc ttcatcggtt
     8641 cgatacgcgg caaacaccgc ctaaacctgc ccgccgtcat tgccgaaatc aaaaaggcaa
     8701 gcccgagcaa aggcctgatc cgtccggact tccgaccggc cgagattgca cgcgcctatg
     8761 aaaacgccgg agcggcgtgt ttgtccgtac tgaccgacga accctatttc caaggttcgc
     8821 ccgaatacct caaacaggcg cgcgaagccg tatcgctgcc cgtgctgcgc aaagacttca
     8881 tcatcgacga atatcaggtt tatcaggcgc gcgcatgggg ggcggatgcc gtcctgctga
     8941 ttgccgcagc actggaacag gaacaattgg aacgctttga agcggtggcg cacgaattgg
     9001 gcatgaccgt cctgctcgag ctgcacaatg aaaccgaatt ggaaaaatgc cgcaacctga
     9061 ccacgccgtt gtggggcgta aacaaccgca acctgaggac ttttgaagtc tccctcgacc
     9121 aaaccctgtc gctgctgccc gcactggaag gcaaaaccgt cgttaccgaa agcggcatta
     9181 cgggtaaggc ggatgtggaa tttatgcaat cgcgcggcgt gcacaccttc ctaatcggcg
     9241 aaacgttcat gcgtgccgac gatattgagg cggaagtggg caaactcttc taaatcccga
     9301 tttcagacgg catattgccg ccaaccgacc aacaccctta ccgagcacgg aaccatcatg
     9361 caccgaccga ccgccaaaca gattctgcac gaagtatttg gttatcccga atttcgcggc
     9421 aggcaggagg ctgtcatcaa tactttggca ggcggcggga gtttgacggt gctgatgccg
     9481 acgggcggtg gcaagtcttt gtgttaccag attcccgcgc tgatgcgcga aggcgtggcg
     9541 gttgtcgtat cgccgctgat tgcgctgatg aacgaccaag tggcaaacct gcacgccgcc
     9601 ggcatcgaag cggcggcagt caacagcggc acatcggcag acgaggcgcg cgagattgcc
     9661 gaccggcttg cccaaggccg tctgaagctg ctttatgtcg cgccggaacg cttggttacc
     9721 gaccgctttt tgcgttttct cgaccaacaa accgtcagtc tgtttgccat tgatgaggcg
     9781 cattgcgtca gccaatgggg acacgatttc cgccctgaat atcaacagct cggcatgctt
     9841 gccgaacgct atccgaacgt cccgcgcatc gctctgaccg ctaccgccga tgccgccacg
     9901 cgcgccgaca tcaagcatta tctgcacttg gacgatgcgc ccgaatttgt ctccagcttt
     9961 gaccgtccga atatttatta tcaggttatc gaaaaaaaca acggcaaaaa acaattgctg
    10021 gatttcatcc gcaaagaaat gacggggcaa agcggcattg tgtattgcct aagccgcaaa
    10081 aaggtggaag atgtggcgca gtttttgcgt gaaaacggat taaacgcgat tccgtatcat
    10141 gctggtttga gcatggacgt acgcgaggaa aaccaacgcc gctttacgca tgaagacaat
    10201 attatcgtgg tggcgaccgt ggcgttcggc atgggcatag acaaacccga cgtgcgcttt
    10261 gtcgcccatc tcgatatgcc ccagagtgtc gaacatttct atcaagaatc ggggcgcgcc
    10321 ggacgggacg ggctgccggc cgcaagctgg ctgtgttacg gtttgaacga ttgggtgttg
    10381 ctgcgcgaac ggattgccga aggcaacagc gacgaggtgc aaaagcaaat cgaaatgcaa
    10441 aaactcgatg ccatgcttgc cgtctgcgaa accgccgcct gccgccgcgt actgctgctc
    10501 aaacatttcg gcgaagcatc cgaaccctgc ggccattgcg acaactgcct gcatccgccc
    10561 gtacggtttg acggcacggt gttggtgcaa aaattactca gctgcgtgta ccgcgccgga
    10621 caacgttttg ccgccggtta catcaccaac attttacgag gtaaaagcga cgattggata
    10681 cgcggcaacc ggcacgaaca actgtccaca ttcggcatcg gtacggagtt gtccgacaaa
    10741 gaatggcgca gcgtcatccg ccagtgtatc agcctcggct acctcaccgt caacattacc
    10801 cgatatcagg cattgcaact gaccgaagcc gccaaaaaag tcctcaaagg cgaaaccgaa
    10861 gtgatgctgc gtccgctcaa gcgcgacaag cccgccgccc gcaccctcaa agacaactgg
    10921 ctgcgtaccg aacgcgaaga acgcctgtgg caggcattgc gcgtttggcg tatgaaacag
    10981 gcagaagccg aaggcatccc cgcctatatg attttcggcg acaaaaccct gcgcgacctt
    11041 gtcgaaaaaa tgccgcaaaa cctcaacggg ctgcacgaca tctacggctt gggcgaagcc
    11101 aaaaccgaac gtttcggaca cggcatactc gaagtctgcc gcaacgccgc cggcttcagc
    11161 cgcgacgcgg tcatccgtcc gcaaaccgaa cgcgaacaac aactgcgtca aaaactcgag
    11221 gcctggcggt atgaacaggc aagggcggaa aactgcgccc tgcatgccgt cctctccgac
    11281 gaaagccttg ccgatatgct tgccgccacg cccgaaaccg aaaccgacct cgaaggcgta
    11341 cacggcttgg gcagcgtacg cgccgccaaa tacggatggg acatcctcgc cgtctgccgt
    11401 ccgttttcag acggcatcga tgaaaccgcc aaacacaaac gccgcctgat gcgcgccctg
    11461 attcaatggt gcaacgaaac cgcaaaacac gaacagtccg aaccctaccg cattctcagc
    11521 aaagccgccc tacgcgccat tgccgccaaa cagccggaag gtttggcgga gcttgccgcc
    11581 gtatatggcg taggcgaaga aaaagccgca cgttacggtg cggcggtgtt ggcggtgttg
    11641 ggacaaaatg ccgtctgaaa accgttacca agtttcagac ggcattgcct ctatttaaaa
    11701 attcctg
//
LOCUS       DAXHNE010000048        11638 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.048, whole genome shotgun sequence.
ACCESSION   DAXHNE010000048 DAXHNE010000000
VERSION     DAXHNE010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11638
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.048"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(339..761)
                     /locus_tag="ACFM4Z_000999"
     CDS             complement(339..761)
                     /locus_tag="ACFM4Z_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFC6021053.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGKAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(879..1550)
                     /locus_tag="ACFM4Z_001000"
     CDS             complement(879..1550)
                     /locus_tag="ACFM4Z_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HFC6021054.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDAVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QAAKP"
     gene            complement(1566..2192)
                     /gene="purN"
                     /locus_tag="ACFM4Z_001001"
     CDS             complement(1566..2192)
                     /gene="purN"
                     /locus_tag="ACFM4Z_001001"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFC6021055.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIHNVRIAAVLSNSETAAGLQWA
                     AERGIPTDSLNHKNFTSRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCARYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGVVPILDGDTA
                     DDIAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2299..3117)
                     /locus_tag="ACFM4Z_001002"
     CDS             complement(2299..3117)
                     /locus_tag="ACFM4Z_001002"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6021056.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3376..3816)
                     /locus_tag="ACFM4Z_001003"
     CDS             complement(3376..3816)
                     /locus_tag="ACFM4Z_001003"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFC6021057.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSEPSVLLACGGNLPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEELADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3879..5285)
                     /locus_tag="ACFM4Z_001004"
     CDS             complement(3879..5285)
                     /locus_tag="ACFM4Z_001004"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFC6021058.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEASQLSNPTVLALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDVHVFEEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHTAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSAEA
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEDYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5456..6571
                     /gene="lptF"
                     /locus_tag="ACFM4Z_001005"
     CDS             5456..6571
                     /gene="lptF"
                     /locus_tag="ACFM4Z_001005"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFC6021059.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGEFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFAVALILLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6568..7638
                     /gene="lptG"
                     /locus_tag="ACFM4Z_001006"
     CDS             6568..7638
                     /gene="lptG"
                     /locus_tag="ACFM4Z_001006"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFC6021060.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELIPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7848..10433
                     /gene="acnB"
                     /locus_tag="ACFM4Z_001007"
     CDS             7848..10433
                     /gene="acnB"
                     /locus_tag="ACFM4Z_001007"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFC6021061.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLASIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10575..11570
                     /locus_tag="ACFM4Z_001008"
     CDS             10575..11570
                     /locus_tag="ACFM4Z_001008"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFC6021062.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEIFESPASIVFDQAENRMHTIKAVMVAALGD"
BASE COUNT         2675 a         3241 c         3023 g         2699 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactataatt caataaatta atatatggct taaaataacg
       61 ggattctcgc ctcccgcccg cccgcagaag caggcgggta tcattttaaa acgcggcatt
      121 taaaatttga ccgaaaattg ttgacaatcc ggaatcaagt ctgcacaata ccccgacaag
      181 tccaagtatt ataaaggctg aataaagagg aaacagcagg cagatatatt cgggaggtgc
      241 agtccgaata tatctgcttt tttatgcgcc tccggattgc ctgccgcacc tttcccttca
      301 gacggtatca gccgtttccc cataatgccg cccgatgcct atttatctgc cccggcaatt
      361 tcaaaactgt gggtaatctt tgccgctttg cccaacataa tcgaagccga acagtatttt
      421 tcggcagaca tctgaacggc gcgctcaatg gccgattctt tcaaatcatg cccgaatact
      481 ttgaaatgga tgtggatttc ggtaaacacg cgcggcgcat cgtccgcccg tttcgccgta
      541 accgtcgcac ggcagtcagt cactttctga cgctgttttt cggcaatcat caccacatcg
      601 atgctcgaac agcccgccac gcccaacagc agcatttcca aagggctggg cccgcgctta
      661 gccttacctt ctgccgccga cccctccata acgacgctgt gcccgccttc cgtcgtgccg
      721 acaaaacaca tcccgtctat ccattttgat gtaacctgca tggtgtcatt cctgaaaata
      781 gcgttaaaac cgctttgcat atggcgttat tgtaaacaat ttcaagcggc ttatgcagaa
      841 atatggacaa aacggcaaaa aaacacttga aaaccgattt acggtttggc tgcctggccg
      901 ttgatctgca ccgatttgag tttcagcgta taggttttgc cgtcgtcggt atagccgatt
      961 tgtgccggaa tattgttcag ggacggtgcg aagaaataca ttaccgcatc gtcgccgcgc
     1021 cgcacccgat atttgacgac ttcggtttcc acgccgccta tgctgtattt tcctgtaccc
     1081 gccttattca aaccgccgac ggaataaagt tttttgccgt tggtgatttt cagccccggg
     1141 gggagtttcg cgtcatttgc cgccaactgc caggcaagcg tgaacaaatc catagccttg
     1201 gggctttgct cggttttgct ctcgcccgct ttgccgtaag ttacgctgcc gtcggcgaat
     1261 ttggcttccg catacagttt gcccctgcgt atgtctctat agtaggtagg gtgcagggta
     1321 ttgccgacaa ccgtaccgcc ggactcgaaa cggatattgt atagcggcac tttaatcgtc
     1381 gaaacgattt tgtaagcatt gccgctgcgt tcaaatgtca tcgtggcggg aatgccgtag
     1441 ctgccggaat agtgcagcac ggcggattgg ggcagccctg ccgcatacgc gcacggcagg
     1501 gcggcggaca aaatggcggc ggaaaatata tttttaaaag tcttcatcat ttgctcccgc
     1561 ccggtttacg ccgtcagaaa acgggcggca tcggcgtttt ccgaatttct gacgcggttt
     1621 ccctcaataa tcaggcggcc ggcggcaaaa tcggcaacgg ctttcggata aagtttatgc
     1681 tcgacagcca aaacccgtgc ggcaatatcg tctgccgtat cgccgtcgag tatcggcaca
     1741 accccttgcg atacaatcgg gccgcaatcc agttcggcag taacgaaatg gatggtgcag
     1801 ccggcaacgc ggcagcccgc ctccaaagcg cgttcgtgcg tatgaagtcc ggtaaacgag
     1861 ggaaggatgg acgggtgaat gttcatcagc ctgccttcgt aacgggcgca aaactcgggg
     1921 gtcagaatcc gcataaaacc tgccaaaacc accaagtcgg gttgatatgc gtcgattttc
     1981 tccatcatgg cggtatcgaa ggcaagccgg gatgtaaagt ttttatgatt caggctatcg
     2041 gtcgggatgc cgcgttcggc cgcccattgc aaaccggcag ccgtttcgct gttgctcaac
     2101 acggcggcaa tgcggacgtt gtgaatggcg gcattgacga ttgcctgcat attgctgccg
     2161 cgtccagaaa tcaggatgac gatgtttttc ataatggtgc gcttttgaaa gggatgccgt
     2221 ctgaaccgct gtttggtggt ttcagacggc atttgccgta aaaatgcccg aaaacctgtt
     2281 tcgggcatgg attcggactt aatttacttt tttgatgtcg acttgagccg gctgcttggc
     2341 gggcgcgttt tcgggtgcgc cgattttgac cagtttcaca tcaaatacca aagtggcgtt
     2401 cggaccgatt ttgtcgcccg caccctgttc gcggtaggca aggttggacg ggatgtagaa
     2461 cgtggcttcg ccgccttctt tcagaagctg tacgccttcg gtccaacccg gaatcacttg
     2521 gctcaaaggg aaggtgaccg ggccgccgtt ggctttgctg ctgtcgaata ccgtaccgtc
     2581 aatcaggcgg ccttcgtatt ccacggtaac gatgtcgtct ttggtcggct gtttgccttc
     2641 gccctgtttg gtgattttgt attgcaggcc ggaagcagtg gtcttcacgc cgtctttggc
     2701 ggcattttct ttcagaaagg cttcgccttt ttctttattg gccttcgcgt ccgccttgtg
     2761 tttttctacg gctttagcct gttgttcctg aaggaatttc atcatgactt cctgagcctg
     2821 ctcttcggtc attttgattt ctttgccgtc atacactgcc tgcatggctt cggtaaagac
     2881 tttcaaatcg atttccgcgc cctgttcctt catttgcttc agggagcgtc cgatgtccac
     2941 gcccatcgca tagcttgcct gctgcatcgt gctgccgatc gaagaggtgt cgccctgcgc
     3001 ggaagaagcg gcggcaggtt cggatgcaga tgcgggggcg gcttcttttt tgccgcaggc
     3061 ggaaagtgcc aaagcggcgg aaagggtcag tgcgctgatt ttgaaaatgg tgttcatgat
     3121 ggatcttcgc tgtcgataag gtcggaaaaa cgggattata gccgagtttg aatgtttcaa
     3181 cacacaggat gacacataaa gcgtcaatcg tgtgttgccc tgttttggaa gggattgaac
     3241 cttccaaaat aagttttgat tctaccgccc cgagggacag atgtccaagt ggcggggttc
     3301 aaccgataag gaaattttaa tcaaatagaa tcaagcctgt ttaaattttg taaatgcggc
     3361 atttcagacg gcattttatg ccttgccctc catgccgtga tgttcgatgg caaaaccgct
     3421 tcggcggtag gcggtaaagc gttcgcgcgc gtcggcgagc tcttccaagc tgttgccgac
     3481 gatttccaaa acgcgcgcgg gcaagaccgg agcggtgttc caaaaaccgt cggacaggtt
     3541 caaaacggtc atgccttcgg gaattcgggg cagattgccg ccgcaggcaa gcaggacgga
     3601 tggttcagac ggcatggctt cttccgtttc ccaaatttcg tgcggaatga aactctcggc
     3661 ctcgtattgc caaagcattt tgtccaattc ctgaagctgc ccgaacgaat cggaccacac
     3721 cagtatcctg ccgccgtccc gaatcgcacg ggcaatcagg cggcaggtga aaatcggaac
     3781 ttgggcaacg tgcgtgtaaa aggtggcttt cggcatattg tttgaacatt tggcaggata
     3841 atgccgtctg aaaggcttca gacggcattg tggcaaaatt aaagattccg cagatagttc
     3901 agcagcaagg gaacgggacg gccggtcgca cctttttccg caccggattt ccacgccgta
     3961 cccgcgatgt caaggtgtgc ccatggatag tcttcggtaa agtaggatag gaatgttgcg
     4021 gcggtaatcg tgcccgcgcc gggcgtgccg atgtttggaa tgtcggcaaa gttggatttg
     4081 agttggtctt tgtaggtctc aaagagcggc agttgccatg ctttgtcgtc cacgttgtag
     4141 gaagcggcaa gcaggctgtc gatcaaatcc tgattgttgc ccatcacgcc gctgacatcg
     4201 tgccccaagg caacaataca cgcgccggtc agggtggcga cgtcgatgac ggctttgggt
     4261 ttgaactgct cggcgtaagt gagcgcgtcg cacaaaatca gacggccttc ggcatcggtg
     4321 ttcaacactt cgatggtcag ccctttcata cttttcacga catcgcccgg tttgtttgcc
     4381 gcgccggaag gcatattttc acaagtggcg acgacggcaa tcaggttaat cggcagttgc
     4441 agtttgacgg cggcgcagaa ggtgctgatg acggttgccg cgccgcacat atcaaacttc
     4501 atttcgtcca tgttcaggcc gggcttgagg gagatgccgc cggtgtcgaa ggtaatgcct
     4561 ttgccgacca ataccacagg cgcggcttct ttgtcggctg caccgaaata gctcagttcg
     4621 accaaatagg gggcttccgc gctgcctttg gcgaccgacc aaaacgaacc catgttttct
     4681 ttgatgtagt ctttttcgat gattttggcg tgcgcgccca gtttttcggc ttcggctttg
     4741 gcggtgcgcg ctaaaaattc gggcgtgcat tcgttgggcg cggcgttgcc caagtcgcgg
     4801 cagaggcttt gtccgtaaac ttgcgcttcg gcgacgcgca aggcttcttt gacggcggct
     4861 tcgtgcgcgg tatggaacac ggcagtttca aatttggcgg gcttggcttc ttttttgtag
     4921 cggtcgaaac ggtaggcggc attgccgaac gcaatcgcaa acgcttcggc aacggctgca
     4981 gcctgcgctt cttcaaagac gtgaacgtcc acattgaccg tttcctgatt ttgcgcccat
     5041 ttggcggctt cggcggcggc cttgttcaat gcggcgcggc cggtgctttt cagacagcat
     5101 acggcaacag cctgcaaacc gttgcctgtc gggatttttg tgtcggcaaa attttgacct
     5161 tcttcaagcg aagacaaaag ggcaaggacg gtcgggttgc tcagttgcga tgcttcggtg
     5221 cagacaaata actgtgcgcc tgcctgctgt tcctgcaaga tttcggtttt tgtgctaaat
     5281 tccacgttta ttctcctgat tgagacggtt gtcggtagtt ttcggacggc ctttcgctca
     5341 aaagaccgtc tgaagacggc tggcacgatt gtaccccatt tgaagcaccg tctgaaacct
     5401 tgcgcggaca atccgcctgc gccgaaccgc ttaccgcccc cctgaccgcg attctatgat
     5461 ttatcaaaga aacctcatca aagaactctc ttttaccgcc gtcggcattt tcgtcgtcct
     5521 cttggcggta ttggtctcca cgcaggcaat caacctgctc ggccgtgccg ccgacgggcg
     5581 tgtcgccatc gatgccgtgt tggcattggt cggcttctgg gtcatcggta tgacgccgct
     5641 tttgctggtg ttgaccgcat ttatcagtac gttgaccgtg ttgacccgct actggcgcga
     5701 cagcgaaatg tcggtctggc tatcctgcgg attggcattg aaacaatgga tacgcccggt
     5761 gatgcagttt gccgtgccgt ttgccgtttt ggttgccgtc atgcagcttt gggtgatacc
     5821 gtgggcagag ctacgcagcc gcgaatacgc tgaaatcctg aagcagaagc aggaattgtc
     5881 tttggtggag gcaggcgagt tcaacagttt gggcaagcgc aacggcaggg tttattttgt
     5941 cgaaaccttc gataccgaat ccggcatcat gaaaaacctg ttcctgcgcg aacaggacaa
     6001 aaacggcggc gacaacatca tcttcgccaa agaaggtaac ttctcgctga acgacaacaa
     6061 acgcacgctc gaattgcgcc acggctaccg ttacagcggc acgcccggac gcgccgacta
     6121 caatcaggtt tccttccaaa aactcaacct gattatcagc accacgccca aactcatcga
     6181 ccccgtttcc caccgccgta ccattccgac cgcccaactg attggcagca gcaacccgca
     6241 acatcaggcg gaattgatgt ggcgcatctc gctgaccgtc agcgtcctcc tactctgcct
     6301 gcttgccgtg ccgctttcct atttcaaccc gcgcagcgga catacctaca atatcttgat
     6361 tgccatcggt ttgtttttaa tttaccaaaa cgggctgacc ctgctttttg aagccgtgga
     6421 agacggcaaa atccattttt ggctcggact gctgcctatg cacattatca tgtttgccgt
     6481 tgcactcatc ctgttgcgcg tccgcagtat gcccagccag cccttctggc aggcggttgg
     6541 caaaagtctg acattgaaag gcggaaaatg aacctgattt cacgttacat catccgtcaa
     6601 atggcggtta tggcggttta cgcgctcctt gccttcctcg ctttgtacag cttttttgaa
     6661 atcctgtacg aaaccggcaa cctcggcaaa ggcagttacg gcatatggga aatgctgggc
     6721 tacaccgccc tcaaaatgcc cgcccgcgcc tacgaactga ttcccctcgc cgtccttatc
     6781 ggcggactgg tctccctcag ccagcttgcc gccggcagcg aactgaccgt catcaaagcc
     6841 agcggcatga gcaccaaaaa gctgctgttg attctgtcgc agttcggttt tatttttgct
     6901 attgccaccg tcgcgctcgg cgaatgggtt gcgcccacac tgagccaaaa agccgaaaac
     6961 atcaaagccg ccgccatcaa cggcaaaatc agcaccggca ataccggcct ttggctgaaa
     7021 gaaaaaaaca gcattatcaa tgtgcgcgaa atgttgcccg accatacgct tttgggcatc
     7081 aaaatttggg cgcgcaacga taaaaacgaa ttggcagagg cagtggaagc cgattccgcc
     7141 gttttgaaca gcgacggcag ttggcagttg aaaaacatcc gccgcagcac gcttggcgaa
     7201 gacaaagtcg aggtctctat tgcggctgaa gaaaactggc cgatttccgt caaacgcaac
     7261 ctgatggacg tattgctcgt caaacccgac caaatgtccg tcggcgaact gaccacctac
     7321 atccgccacc tccaaaacaa cagccaaaac acccgaatct acgccatcgc atggtggcgc
     7381 aaattggttt accccgccgc agcctgggtg atggcgctcg tcgcctttgc ctttaccccg
     7441 caaaccaccc gccacggcaa tatgggctta aaactcttcg gcggcatctg tctcggattg
     7501 ctgttccacc ttgccggacg gctcttcggg tttaccagcc aactctacgg catcccgccc
     7561 ttcctcgccg gcgcactacc taccatagcc ttcgccttgc tcgccgtttg gctgatacgc
     7621 aaacaggaaa aacgttgaac caatgccgtc tgaacctctc ttcagacggc atttgttttc
     7681 attgacacat tcccacagac agatagccgt tccctattac attacctgtc ataacagttc
     7741 catttttgtt aaaactagtc tatgatagcg gtacaaatat tgtttacaat atttaacgca
     7801 aatcatttgc aacccgacaa aagaaaaaca gaaaaaggaa caaagagatg ttagaagcct
     7861 atcgtaaagc cgccgccgag cgcgccgccc tcggcattcc cgccctccct ttgaacgcgc
     7921 agcaaaccgc cgatttggtt gagctgctga aaagcccgcc cgcaggcgaa ggcgagttct
     7981 tggtcgaact gcttgcccac cgtgttccgc ccggtgtgga cgatgccgcc aaagtcaaag
     8041 cctcattcct ggctgccgtt gccgaaggca gcgcgtccag cccgctgatc tcccccgaat
     8101 atgcgaccga actcttaggt acaatgctcg gcggttacaa tattcacgcc ttaatcgaac
     8161 tcttggacga cgacaaactc gcgtccattg ctgccaaagg cttgaaacat acgcttctga
     8221 tgttcgattc cttccacgac gttcaagaaa aagccgaaaa aggcaacaaa tacgcgcaag
     8281 aagttttgca atcttgggca gatgccgaat ggttcgcctc acgcgccaaa gttcccgaaa
     8341 aaatcaccgt taccgttttc aaagttgacg gcgaaaccaa tacagacgac ctctcccccg
     8401 cgcccgacgc gtggagtcgt cccgatattc cgctgcacgc gctggccatg ctgaaaaacc
     8461 cgcgcgacgg catcacgccc gacaaaccgg gcgaagtcgg tccgattaaa ttgttggaag
     8521 aactcaaagc caaaggccat ccggttgctt acgtcggcga cgtggtcggt actggttctt
     8581 cacgcaaatc cgcgaccaac tccgtcattt ggcataccgg cgaagacatt ccgttcgtgc
     8641 cgaacaaacg cttcggcggc gtatgtttgg gcggcaaaat cgcgccgatt ttcttcaata
     8701 cccaagaaga ttccggcgcg ctgccgattg aagtcgatgt atctgctcta aaaatgggcg
     8761 atgtcgtcga tatcctgcct tatgaaggca aaatcgtgaa aaacggcgag actgttgccg
     8821 agtttgaatt gaaatcacaa gtattgctgg acgaagtgca agccggcggc cgtatcaacc
     8881 tgattatcgg ccgaggtctg accgccaaag cgcgcgaagc cctgaaactg cctgcctcta
     8941 ctgcattccg cctgccgcaa gcgcctgccg aaagcaaagc cggtttcacc ttggcgcaaa
     9001 aaatggtcgg ccgcgcctgc ggtctgcccg aaggacaagg cgtgcgcccg ggtacttact
     9061 gcgaaccgcg tatgacgacg gtcggctcgc aagacacgac cggcccgatg acccgcgacg
     9121 agttgaaaga cttggcttgt ttgggcttct ccgccgatat ggtgatgcag tctttctgcc
     9181 acaccgccgc ctatccgaaa cctgtcgatg taaaaaccca taaagaactg cccgccttta
     9241 tttccacccg tggcggcgtg tcactgcgtc cgggcgacgg cgtcatccac tcgtggctca
     9301 accgcctgct gctgcccgat accgtcggca ccggcggcga cagccatacc cgtttcccca
     9361 tcggtatttc cttccccgcc ggctccggct tggttgcctt tgccgccgca acgggcgtaa
     9421 tgccgctcga tatgcccgag tctgtattgg tacgcttcag cggcaagctg caaccgggcg
     9481 taaccctgcg cgatttggtg aacgccatcc cgctgtacgc aatcaaacaa ggtttgctga
     9541 ccgttgccaa agccggtaag aaaaacatct tctccggccg catcctcgaa atcgaaggcc
     9601 tgcctgattt gaaagtggaa caagcctttg aattgaccga cgcatccgcc gaacgctccg
     9661 ccgccggctg taccgtgaag ctcaacaaag agccgattat cgagtacatg aaatccaacg
     9721 tcgtgttgat gaaaaacatg attgccaacg gctatcaaga cccgcgcact ttggaacgcc
     9781 gcatcaaagc tatggaaaaa tggctggcaa atcccgagtt gctcgaagcg gataaagatg
     9841 ccgaatacgc cgccgtgatt gaaatcaaca tggacgacat caaagagccg attatcgcct
     9901 gcccgaacga cccggacgac gtgtgcttca tgtccgaacg ctccggcacc aaaatcgacg
     9961 aagtattcat cggttcgtgt atgaccaaca tcggccactt ccgcgccgcc tccaaacttt
    10021 tggaaggcaa ggcagacacc cccgtccgcc tgtggattgc gccgccgacc aaaatggacg
    10081 cgaaacaatt gtccgacgaa ggacactacg gcgtactcgg acgtgccggc gcgcgtatgg
    10141 aaatgccggg ttgctcctta tgtatgggta atcaggcgca agtacgcgaa ggtgcgaccg
    10201 ttatgtccac ctccacccgc aacttcccga accgtttggg taaaaacacc tttgtttacc
    10261 tcggttcggc ggaattggca gcgatttgct ccaaactggg taaaatcccg accgttgaag
    10321 aatatcaagc caatatcggc atcatcaacg aacagggcga taaaatctac cgctatatga
    10381 acttcaacga aatcgacagc tacaacgaag tagccgagac cgtgaacgtt taatccccgt
    10441 catccgtatg aagtaaggga ttgaccgcaa tgccgtctga acaaccttca gacggcattg
    10501 caacattccg ctaacccttc tttccgcaaa cgctgcaaat acggcgttca cgcccccaca
    10561 taaaggaaac gacagtgaac ctgaaaaacc gccattttct gaaactttta gacttcacgc
    10621 cggaagaaat caccgcctac ctcgaccttg ccgccgaatt gaaagccgcc aaaaaagcag
    10681 ggcgcgagat tcagcggatg aaagggaaaa acatcgccct gatttttgaa aaaacctcta
    10741 ctcggacgcg ctgcgcgttt gaagtcgccg cgcgcgatca aggcgcggga gtgacttatt
    10801 tagagccgtc cgccagccaa atcgggcata aggaaagcat caaagacacc gcccgcgtgt
    10861 tgggcaggat gtacgatgcc atcgaatatc gcggtttcgg tcaggaagtt gttgaagaat
    10921 tggcgaaata cgcgggcgta cccgtgttca acgggctgac caacgagttc catcccacac
    10981 aaatgcttgc cgacgcactg actatgcgcg aacacagcgg caaacctttg aaccaaaccg
    11041 cgtttgccta cgtcggcgac gcgcgttaca acatgggcaa ttccctgctg attttagggg
    11101 caaaattggg gatggacgtg cgtatcggcg caccgcaaag cctgtggccg tctgaaggca
    11161 ttattgccgc cgcacacgcc gccgccaaag aaaccggcgc aaaaattacc ctgaccgaaa
    11221 acgcgcatga agccgtgaag aatgttgatt ttattcatac cgatgtgtgg gtcagcatgg
    11281 gcgagccgaa agaagtctgg caggaacgca tcgatttgct gaaagattac cgcgttacgc
    11341 ccgaactgat ggcggcatcg ggcaatccgc aagtcaaatt catgcactgc ctgcccgcct
    11401 tccacaaccg cgaaaccaaa gtcggcgaat ggatttacga aaccttcggg ctgaacggtg
    11461 tggaagttac agaagaaata ttcgaaagcc ccgccagcat cgtgttcgat caggcggaaa
    11521 accgtatgca cacgattaaa gcggtaatgg tcgcggctct gggcgactga cagaactgtg
    11581 cctgtttaaa ttcatccgca acacagatac cgtctgaaca cgatgttcag acggtatc
//
LOCUS       DAXHNE010000049        11630 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.049, whole genome shotgun sequence.
ACCESSION   DAXHNE010000049 DAXHNE010000000
VERSION     DAXHNE010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11630
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.049"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            46..285
                     /locus_tag="ACFM4Z_001009"
     CDS             46..285
                     /locus_tag="ACFM4Z_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021063.1"
                     /translation="MNVWDIWGRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEELCIG
                     RCYAPMSESPDFANAVAMAVAMICIVWIAVLSMTD"
     gene            499..1800
                     /gene="bioA"
                     /locus_tag="ACFM4Z_001010"
     CDS             499..1800
                     /gene="bioA"
                     /locus_tag="ACFM4Z_001010"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFC6021064.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLRAKQNMATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNLTSRFDDEWDEADLQPVRALFEAHHTDIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKCRLKTAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
     gene            1797..2444
                     /gene="bioD"
                     /locus_tag="ACFM4Z_001011"
     CDS             1797..2444
                     /gene="bioD"
                     /locus_tag="ACFM4Z_001011"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFC6021065.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKPVQTGC
                     QNIADDIAVHRKIMGKPMQEADKQGLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLYSLVFNHIHDSRDAHIAQDSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            2411..2641
                     /locus_tag="ACFM4Z_001012"
     CDS             2411..2641
                     /locus_tag="ACFM4Z_001012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021066.1"
                     /translation="MDGVGKNRRGIKIGKNMEHLYDEYGNTTPNRRTTNKKDETISSK
                     VTVAAIPVAAPFAIADIVDQDFIQAIMKITGH"
     gene            2665..3051
                     /locus_tag="ACFM4Z_001013"
     CDS             2665..3051
                     /locus_tag="ACFM4Z_001013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021067.1"
                     /translation="MKCYIKVVIIVFIMVMTTSCNPCFFATSSCDYSFRKNYPINSDD
                     EYYVKNNTSKETRIADLRYCISYACPEVYQKYQLPDENGDYREIIRRKYKDDYKKGYK
                     NADDVMTCYGKIGECMKNNKGYSTKW"
     gene            3045..3488
                     /locus_tag="ACFM4Z_001014"
     CDS             3045..3488
                     /locus_tag="ACFM4Z_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021068.1"
                     /translation="MVMFRLLSTALVLLIMQGCYYTTIDNEKCFVAPQSVHCPVSSDI
                     ENVIRKNPNKSINQVRSDIKYCANMYFSNIRELNIPQDFDNYIFMLKEKGIPRNDKEL
                     AYNAVRNMHIICLSSLGGYDYYQKTAEFQKIDYKKEMEKLLNENH"
     gene            3559..3942
                     /locus_tag="ACFM4Z_001015"
     CDS             3559..3942
                     /locus_tag="ACFM4Z_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021069.1"
                     /translation="MITRCFKVVIIVFIMVMTTSCNPCFFATSSCNYPFDDMTIRANN
                     DIEYFVKNNTTNEQRIDDFRSCLSVACPEIYKLYQLPDEKGNYQYIIKKYYDNKYNDN
                     AVKSTNCIGDNARCMLNNKGYSTKW"
     gene            3945..4421
                     /locus_tag="ACFM4Z_001016"
     CDS             3945..4421
                     /locus_tag="ACFM4Z_001016"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFC6021070.1"
                     /translation="MGYGFEEWLPDLPAPVSDGIGLPMSRLLKARSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVAARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            4451..5341
                     /gene="ubiA"
                     /locus_tag="ACFM4Z_001017"
     CDS             4451..5341
                     /gene="ubiA"
                     /locus_tag="ACFM4Z_001017"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFC6021071.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVGKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVPPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            5526..5975
                     /gene="ptsN"
                     /locus_tag="ACFM4Z_001018"
     CDS             5526..5975
                     /gene="ptsN"
                     /locus_tag="ACFM4Z_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFC6021072.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLIFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            5977..6939
                     /gene="hprK"
                     /locus_tag="ACFM4Z_001019"
     CDS             5977..6939
                     /gene="hprK"
                     /locus_tag="ACFM4Z_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFC6021073.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQHNENRPD"
     gene            6920..7774
                     /gene="rapZ"
                     /locus_tag="ACFM4Z_001020"
     CDS             6920..7774
                     /gene="rapZ"
                     /locus_tag="ACFM4Z_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFC6021074.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSDR"
     gene            7855..8796
                     /gene="ylqF"
                     /locus_tag="ACFM4Z_001021"
     CDS             7855..8796
                     /gene="ylqF"
                     /locus_tag="ACFM4Z_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFC6021075.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKIWLEHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGSVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            8897..9499
                     /locus_tag="ACFM4Z_001022"
     CDS             8897..9499
                     /locus_tag="ACFM4Z_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFC6021076.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEEPEAVPSDTLPN"
     gene            9683..11356
                     /gene="recN"
                     /locus_tag="ACFM4Z_001023"
     CDS             9683..11356
                     /gene="recN"
                     /locus_tag="ACFM4Z_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFC6021077.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPTLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSRAQAET
                     VRQLYQNWANSKKALQEAQEQAENIALEQERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRSLGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDKIQRVEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            11466..11542
                     /locus_tag="ACFM4Z_001024"
     tRNA            11466..11542
                     /locus_tag="ACFM4Z_001024"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11500..11502,aa:Arg,seq:acg)
     gene            11560..11630
                     /locus_tag="ACFM4Z_001025"
     tRNA            11560..11630
                     /locus_tag="ACFM4Z_001025"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11593..11595,aa:Glu,seq:ttc)
BASE COUNT         3005 a         2738 c         3032 g         2855 t
ORIGIN      
        1 ggctgtaacc ttcaatcgga atttgttgcc tgcgggatat gcggtatgaa tgtttgggat
       61 atatggggca ggatggtgga aatctatcat aagtataaga agccgtgcct ggttttggcg
      121 gtggattttg tgatgggtat ggtattcata gagccgaatg aggagctgtg catcggtaga
      181 tgctatgcgc ctatgtcgga gtcccctgat tttgctaacg ctgttgcgat ggctgttgct
      241 atgatctgta tcgtatggat tgccgttttg tctatgactg attgaagcgt tgggcggagg
      301 ctgtgtgaaa caatattggg cgttgggtcg tctgattcca atcgggcttg gggaatgcga
      361 aacggtgtgc gcttatactg cggacgattt gttgcgtggt tttacgcccg aaacggatgg
      421 aaaggtgtgg gaagcggtct gtcggagtag aatacgcgtt ttgcgtttga atgcagcaat
      481 aagaaaagag agaaacttat gccgtctgaa catcaacaca catcatcatt gcttgatttc
      541 gaccgtaccc atctgctcca tccctatact tccatgaccg atccgcttcc cgtttatcct
      601 gtcaaacgtg cagaaggggt gtttatcgaa ttggcggacg gcacgcggct gattgacggg
      661 atgtcgtctt ggtggtgtgc gatacacggt tacaatcatc ccgttttgaa tcaggcggtt
      721 gagacgcaga tgaaacagat gtcgcatgtg atgttcggcg gtttgacgca tgagccggcg
      781 gtggagctgg gcaaattgtt ggtcgggatt ttgccgcagg ggctggatcg tattttttat
      841 gcggattcgg gttcggtttc ggtggaagtc gcgttgaaga tggcggtgca ataccagcag
      901 gcgcggggtt tgcgtgcaaa acaaaatatg gcgacggtgc ggcgcgggta tcacggcgat
      961 acttggaacg cgatgtccgt ctgcgatccg gaaacgggga tgcaccatat tttcggcagc
     1021 gcgttgccgc agcgttattt tgtcgataac ctgacaagcc gtttcgacga tgaatgggac
     1081 gaggcggatt tgcagcctgt ccgcgccttg tttgaagcgc atcatacgga cattgccgcc
     1141 tttattttgg aaccggtcgt gcagggcgcg ggcggtatgt atttttatca tccgcagtat
     1201 cttcgcggat tgcgcgattt gtgcgacgag tttgatatcg ttttgatttt tgatgaaatc
     1261 gccaccggat tcgggcgcac gggcaagatg tttgcctgcg aacacgcgga ggtcgtgccg
     1321 gatattatgt gcatcggcaa gggtttgagc ggcggctata tgacgctggc ggcagcaatc
     1381 acttcgcaaa aagttaccga aacgatttcg cgcggcgaag cgggcgtgtt catgcacggc
     1441 ccgacattta tggcgaaccc gctggcgtgt gccgttgcct gcgcttcggt caaactgctt
     1501 ttgtcccaag attggcaggc aaatatccgc cgcatcgaaa gcatcttaaa atgccgtctg
     1561 aaaaccgcgt gggacattcg tggtgtgaaa gacgtgcgcg ttttgggcgc catcggcgtg
     1621 atcgagttgg aaaaaggcgt ggatatggcg cgttttcaag cggattgcgt ggcgcagggc
     1681 atttgggtgc gcccgttcgg caggttggtg tatctgatgc cgccttacat catttcagac
     1741 ggcgttttga ccaaacttgc cgacaaaacc gtgcaaatct tgaaggaaca cagcaaatga
     1801 aaggcgttta cttcgtcagc ggcatagaca cggacatcgg caaaaccgtc gccaccggcg
     1861 tgttggcaaa acaattgttg cagcagggca aaagcgtgat tacgcaaaag cccgtgcaaa
     1921 ccggttgcca aaacattgcc gacgacatcg ccgtccatcg taaaatcatg ggtaaaccca
     1981 tgcaggaagc cgacaaacaa gggctgacca tgcctgaaat cttcagttat ccagcttcgc
     2041 ctcatctcgc tgcccgtctt gacggcaggg ctttggactt ggacaaaatc cgcaccgcca
     2101 cacaagaatt ggcggcgcag tacgaagtcg ttttggtcga aggcgcgggc ggattgatgg
     2161 ttccgctgac ggaaaacctg ttaaccattg attatatccg tcagcaaggc tatcccgtca
     2221 tcctcgttac cagcggacgg cttggcagta tcaaccacac tttactcagt ttcgccgcgc
     2281 tcaaacaata cggcattcgc ttgtacagcc tcgtgttcaa ccacatccac gacagccgcg
     2341 acgcacacat cgcccaagac agcctgagct acttgaaatg ccgtctgaaa gccgattttt
     2401 ccgaagcgga atggatggag ttggcaaaaa cagacgcggt ataaagattg ggaaaaatat
     2461 ggaacaccta tatgatgaat atggaaatac aacgccaaat agaagaacaa caaataaaaa
     2521 agatgaaact atttcaagta aagtaactgt tgcagcgata ccagtagccg ctccatttgc
     2581 tattgctgat atagtcgatc aggattttat ccaagcgatt atgaaaatta ctggtcattg
     2641 attatctata tattggtggt tgttatgaaa tgctatatta aagttgtcat tattgttttt
     2701 ataatggtaa tgacaacttc ttgtaatcct tgcttttttg caacttcttc ttgtgattat
     2761 tcttttagaa aaaactatcc tataaatagc gacgatgaat attatgttaa gaataatacg
     2821 agcaaagaaa caagaattgc ggatttacgc tattgtattt cttatgcatg tccagaagtt
     2881 tatcaaaaat atcaactacc agatgaaaat ggagattata gggagattat tcgtagaaaa
     2941 tataaagatg attataaaaa gggctataag aatgcagatg atgtcatgac atgctacgga
     3001 aagattggag aatgcatgaa aaataataaa ggatattcaa caaaatggta atgttccgct
     3061 tattatcaac agcccttgta ttgttgataa tgcagggctg ttattacaca actattgata
     3121 atgaaaaatg ctttgttgcc ccacaaagcg ttcattgtcc tgtaagttct gatattgaaa
     3181 atgttattag aaaaaatcct aataaaagca taaatcaagt taggagtgat attaaatatt
     3241 gtgctaatat gtatttttcc aatatacggg aattaaatat tccgcaagat tttgataatt
     3301 acattttcat gcttaaagaa aaaggtattc caagaaatga taaggaattg gcttataatg
     3361 cagttagaaa tatgcacatt atttgtttga gttctttggg tggttatgat tattatcaaa
     3421 aaaccgcaga atttcagaaa atagattata aaaaagaaat ggagaaattg ttaaatgaaa
     3481 accattaaat ttgctattgc cgatatagtt gatagcgact ttatccaagt gattatgaaa
     3541 ttaagcgggc attgatttat gattacgcgt tgttttaaag ttgtcattat tgtttttata
     3601 atggtaatga caacttcttg taatccttgc ttttttgcaa cttcgtcttg taattatcct
     3661 tttgatgata tgacgataag agcaaacaac gatattgaat attttgttaa aaataatacg
     3721 actaacgaac aacgaattga tgatttcaga agttgccttt ccgttgcttg tcctgaaata
     3781 tataaattat atcaattacc tgatgaaaag ggtaattatc aatatattat aaaaaaatat
     3841 tatgataata aatacaatga taatgcagtc aaatcgacaa attgtattgg ggataatgca
     3901 agatgtatgc ttaataataa aggatattca acaaaatggt aataatgggt tacggctttg
     3961 aggaatggct gcccgacctg cccgcccctg tttcagacgg catcggcctg ccgatgagcc
     4021 ggttattgaa agcccggtcg ctgaccgccg cattgcgcgc cttgccgcat acattttcgg
     4081 tggaactgct gaaactgggc gaattggaga cggaatgcgg agggaggctg gtgcgcgaag
     4141 ttttgttgaa gctggacggt atccctgttg tcgcggcaag gagcgagtgc cgtatcggtt
     4201 cggcgttttg gcaaaacatt ttggactgcg gcacgcgtcc tttgggcgag cgtctgtttc
     4261 aagccgattt ggaaggggcg cgttcggcgt ttgagtttgc cgtttccggc gaaggatgcg
     4321 gacggtactt tgccgcgcgg cgttctcggt tttcccgtca cggcgaggaa atgctgctga
     4381 ccgagtattt tctgcccgaa ctgaaacgct ttatcggata aaataccgtt ttttcaagct
     4441 gtgcggcaat atgaatccta aatccccttt atttttacga ctgtccgatc gtttggatgt
     4501 gtacctgcgc ctgatgcggg cggacaagcc cattgggacg ctgcttttgc tgtggccgac
     4561 ctactgggca ttgtggctgg cttcagacgg cattcccgat ttggcggtat tggcggcgtt
     4621 tacaatcggc acgtttttaa tgcggagtgc cggttgcgtc atcaacgatt ttgccgaccg
     4681 cgattttgac ggtgctgtcg agcgcaccaa aaaccgtccg ttcgcacagg gtcgggtcgg
     4741 aaaaaaagaa gcgctgctgc tgacggcatt tttgtgcctg cttgccgcat tgtgcctgat
     4801 tccgctgaat catctgacat ggctgatgag cctgcccgcg ctgtttcttg cgctgactta
     4861 cccgtttacc aaacgctttt ttccgattcc ccaactctat ctcgggcttg ccttttcctt
     4921 cggtatcccg atggcgtttg ccgccgttgc cggaaacgtg ccgcctcaag cgtggatact
     4981 ctttgccgcc aatgtgttat ggactctggc gtatgacacg gtttatgcaa tggcggacaa
     5041 agaagacgat ttgaaaatcg gcatcaaaac ctccgccgtc acgttcgggc gttacgacat
     5101 cgccgccgtt atgctgtgtc atggcggctt taccctgctg atggcagtat tgggtgcggt
     5161 tatcggtgcg gcatgggcat attggacggc aatccccatc gtcctgctgc tgcaataccg
     5221 ccaatatgcc gccatcaaaa gccgcgtccg gcaaatctgt tttgaaacgt ttttggcaaa
     5281 caacagaatt ggttgggtgt ggtttaccgc catttttgcc catacgtttt tcgcgaaata
     5341 aggcagggca atgccgtctg aagagccgta aactgctttg gacggcattt ctatctgtgc
     5401 cgaaaagcgt taaaatatgt ttttaaaacg ctgtgttatg tcagcccgta ccgtatgcgg
     5461 gattgagatt tgccctagca gtcggtacaa tctttctgtt ttgcgatgtc tgaaaagaga
     5521 agcttatgag ccttatcggc gaaattttgc ctttgtccca tattgttttg gatatggagg
     5581 tgggcagtaa aaaaaggctg tttgaggaag cgggcctgct tttggaacgc gaatcctcat
     5641 tgtcccatgc tgatgttttc gaatgtcttt ttgcccgtga aaaactcggt tcgaccggtt
     5701 tggggcaggg cgttgccatc ccgcacggcc gtcatgccgg cgtgaagcag gcgacgggcg
     5761 cgttcatccg cacgcgcgaa cccgtcggat ttgacgcacc ggacggcaag ccggtttccc
     5821 tgatttttat cttgctggtg ccggaaaacg caactggcga acatttggaa gtcttatcca
     5881 aactggccgg caagttttcc caaaaaagca tccgcgaatc gctgatgacg gtttcttctg
     5941 cggaagaagc ccgcgccatt ttgactgaag aataatatgc ccagtatttc cgtccgccga
     6001 ctgtttgacg acaaccaata caagttacaa cttgcctggg ccgccggcaa ttcgggtgcg
     6061 gacaaccgta tcggcgtaga ggcggacaag cccgtcctcg ctttggtcgg acacctgaat
     6121 ttcatccacc ccaaccaaat ccaagtggtc ggtttggcgg aatcggaata tctgaaccgt
     6181 ctcgaatcgg gggaaacggg ttatcagttc ggcgacctgt tcgacatttc tatgtctttg
     6241 gttattgtgg caaacggctt gccggtttcc ccggggctgc gcgactattg ccataaaaac
     6301 gatattccac tgctgacttc caaactcgaa agcccctatc tgatggacgt gttgcggatt
     6361 tacctgcaac gcaccctggc ggcatcgtcc gtcaaacacg gcgtatttct cgatgtgttt
     6421 gaaatcggcg tgctgattac cggacattcc ggcttgggca agagcgaatt ggcgttggaa
     6481 ctgatttcgc gcggccacag cctgattgcc gacgatgcgg tcgagctgtt ccgcatcggt
     6541 ccggaaacat tggaagggcg ttgttcgcct atgctgcgtg attttttgga agtgcgcggc
     6601 ttggggatac tcaatatccg ccatattttc ggcgaaactt ccatccgccc caaaaaaatc
     6661 ctgcaactca ttatcaattt ggtcgaggca gacgacgagt atatgaagca gctcgaccgg
     6721 ttgagtatcc gcaccgaaac cgaatccatc ctcaacgtca acgtccgttc ggttacgctg
     6781 cccgtcgccg tcggacgcaa cctcgccgtt ttggttgagg cggcggtacg caattatatt
     6841 cttcagttgc gcggtaagga tagtacgcgc gaatttttgg aacgtcatca gacgcagctt
     6901 aaagaaaacg agcaacacaa tgaaaatcgt cctgattagc gggttgtccg gttcgggcaa
     6961 atccgtcgca ctgcgccaaa tggaagattc gggttatttc tgcgtggaca atttgccttt
     7021 ggaaatgttg cccgcgctgg tgtcgtatca tatcgagcgt gcggacgaaa ccgaattggc
     7081 ggtcagcgtc gatgtgcgtt ccggcataga catcggacag gcgcgggaac agattgcctc
     7141 tctgcgcgga ctggggcaca gggttgaagt tttgtttgtc gaggcggaag aaagcgtgtt
     7201 ggtccgccgg ttttccgaaa ccaggcgagg ccaccctctg agtaatcagg atatgacctt
     7261 gttggaaagc ttaaagaaag aacgggaatg gctgttcccg cttaaagaaa tcgcctattg
     7321 tatcgacact tccaagatga atgcccaaca gctccgccat gcagtccggc agtggctgaa
     7381 ggtcgaacgt accgggctgc tggtgatttt ggagtccttc gggttcaaat acggtgtgcc
     7441 gaacaacgcg gattttatgt tcgatatgcg cagtctgccc aacccgtatt acgatcccga
     7501 gttgaggcct tacaccggta tggacaagcc cgtttgggat tatttggacg gacagccgct
     7561 tgtgcaggaa atggttgacg acatcgaaag gtttgttacg cattggttac cgcgtttgga
     7621 ggatgaaagc aggagctacg ttaccgtcgc catcggttgc acgggaggac agcaccgttc
     7681 ggtctatatt gtcgaaaaac tcgcccgaag gttgaaaggg cgttatgaat tgctgatacg
     7741 gcacagacag gcgcaaaacc tgtcagaccg ctaattccgt caaaccatta tgccgtctga
     7801 aaccccgtcc tccgtttcag acggcatttc aaatattgaa aagaaagaac agacatggca
     7861 atccaatggt ttcccggcca tatgaacaag gcgaaaaaag ccatcgccga gcgtgcaaaa
     7921 agcgttgata tggtgattga aatgctggac gcgcgtatgc ccgcctccag cgaaaacccc
     7981 ctgcttgccc agctttccaa aggtaaaccc aaacttaaaa tcctcaacaa acaagatctt
     8041 gccgaccccg agcgcaccaa aatctggctc gaacactata acagccgccc cgacacctgc
     8101 gccatcgccc tcgattcctc cgaaacaggc gcacacggca aaattaccca agcctgtcgt
     8161 gccatgattc cccaccggca aggcatagac aaacccttgc gcgtcttgat ttgcggcatt
     8221 cccaacgtcg gcaaatccac cctcatcaac ggcatgatag gcaaaaaatc cgccaaaacc
     8281 ggcaacgaac ccggcatcac caaagccgaa caacgccttt tccttgccga cgacttctgg
     8341 ctgtacgaca cccccggaat gctgtggccg aaaatcattg tcgaagaaag cggctacaac
     8401 ctcgccgccg gcggttcagt cggacgcaac gcattggacg aagaagaagt cgccctcgaa
     8461 cttttagact acctccgccg ccactacctc cctatgctgc aagaacgcta ccaagccgac
     8521 aaagacccca gcagccactg ggacgaaaac gtttggctcg aatggatagc caaaaaacgc
     8581 ggcgcagtcc tcagcggcgg acggatcaac taccaaaaag ccgccgaaaa catcctcacc
     8641 gacttccgtg aaggcaaaat cggcagaatc accctcgaaa cgccgaacca atgggaaact
     8701 tggctcaaaa aagcccgtca gaaagaagcc gaactcaaag ccatacgcga agccagaaaa
     8761 gcagagcgta aagggcagaa gccttcggaa gcataaagaa tgccgtctga aaaatatttt
     8821 tcaggcagct tctctctact ccaaccgatt tcagacggca tatccaaacc catgccgttt
     8881 cagcacggat acccgtatga ccgacaaaat ttctcccgac gcgctgattg aagccgcact
     8941 gctgacccaa accgaaccgc tgaccgaaaa atctatgcgc gaattgtgtg tgccgccact
     9001 gtcgcaggac aaactgattg atgtgttggc gcagttgaaa acgcgttggc aggatagggc
     9061 gttgcaactg gtgcatacgc aagagggctg gcgttttcag attgttcaga cggcattcga
     9121 gcggctgggc agcctgcaag aacagcgtgc gccgcgctac tcccgcgccg tgatggaaac
     9181 actggcgatt atcgcctacc agcagcccgt aacgcgcggc gacatcgagg gcatacgcgg
     9241 cgtggcggtg tcgcagaacg tgatgcagac cttgcaggat cgggggtgga ttgaagtcat
     9301 cggacatcgg gacacattgg gaaaacccgc attgtgggca acaacggcaa cgttcctcag
     9361 cgatttgcgc ttggacggtt tggaagaact tcctccgctg accgaactgg gcgaactggt
     9421 tttgcccgat ttaatggaaa tgccgcctac ggatgaagaa gagccggaag ccgtaccgtc
     9481 cgataccctg cccaactgaa attccaaatg ccgtctgaaa cgcacattgc ttcagacggc
     9541 attgcaacaa ataagcagat aaaaacaagc attaagaaaa attaaggaaa aacttatttt
     9601 taatttaaaa aatcttagtt ataattcgta tatctaaagt tgatattgct tttgtcggta
     9661 gaattgctaa ggaatcctca cgatgcttct aacactttct ttgcgtgatt ttgtcattgt
     9721 tgaaaatctg aatctggatt ttcaaagcgg ctttaccgta ttgaccggag aaactggcgc
     9781 gggcaagtcc attactttgg atgcgattgg tctgctgttg ggcgataaag ccgattacag
     9841 ccaagtccgc agcggcgcaa aagaagcgca gttgtcggca ttgttcgata tttcccattt
     9901 gcctacttta aaagcagaat tgtatgaaca ggggctttta aacgacggag aagaagaact
     9961 cagtatccgc cgcattatcg atgccaaagg caaaagccgc agctttatca acaatcaggc
    10021 cgctaccttg gcgcaactca aagccgtcgg cagccagctt atcgacatcc acgggcaaaa
    10081 cgcccatcat tcgcttaatc aggaagccgc ccagcgcgaa ttgttggacg catttgcggg
    10141 cagcagggcg caggcggaaa ccgtcaggca gctttatcaa aactgggcaa actcaaaaaa
    10201 agcattgcag gaagcgcagg aacaggcaga aaacattgca ctcgaacaag agcgtctgga
    10261 atggcagttt aacgaattga atcagttgga cattaaacaa ggcgaatggg aagctctcag
    10321 ccaaagccac gacagccttg cccattctgc cgagctgttg caggctgccg aagaagtcgg
    10381 aagcaagatt gacggcgaca acggcatcca acgccatatc tatcaatgtc aaaaactatt
    10441 ggccaatctg caaaacatcg agccgcgctt tgccgagagc ctgaatatgt tggcaagcat
    10501 cgaagccgaa ttgggcgaaa tcagtgccaa tatgcgcgat gtggcaggac gcagcgacat
    10561 caaccccaat gaattggcgg cacaggaaca gcgtatgggc gaactgatgg ggatggcgcg
    10621 gaaataccgg atcgagcctg aagagttgcc tgccaagttg gcagaaatcg aagaacgcct
    10681 gcaaagcctg caagctgccg ccgatttgga cgcgctcgag cataatgttg cccacaattt
    10741 tgccgaatat caggaagctg cccacatcct ttctgccatg cgccatcagg cggcagagcg
    10801 tttgagcggc gaaacgaccg agcatatgca acaccttgcc atgaaaggtg cacgtttcga
    10861 catcgtcctg ttgccctcgt cgccgacggc acacggtttg gagcaggttc aatttcaagt
    10921 tgccgccaac aaaggcaatc cgccccgtcc gctgaataaa gttgcctccg gcggcgaatt
    10981 ggcgcgtatc agccttgcct tacaggttgt tgccagccaa tatacccaag ttcccaccct
    11041 gatttttgat gaggtcgata ccggtattgg agggggagtg gctgaaatgg tcggcaaggc
    11101 attacgttcg ttgggcagaa aacatcaggt gcttgccgtt acccatcttc cgcaagtcgc
    11161 atcctgcggc gaaaaccact ggcgggtgcg caagcacagc gagggagagc aaaccgtcag
    11221 cgaaatcagt atattggata aaatccaacg ggtcgaagag gttgcccgta tgttgggcgg
    11281 agaagtcatt accgatacga cgcggcaaca tgcggcagaa ttgctgcaac ttgcgtcgaa
    11341 aaatagttta ttttaaaatc aatcagttaa aaaataacta aaaataaaag tctaaaacaa
    11401 tagacagaac tcagataaat ccgtattatc acgctttctt aatcacttga acaagtgatt
    11461 gtgctgcacc cgtagctcag ttggatagag tatctggcta cgaaccagag ggtcgggcgt
    11521 tcgaatcgct ccgggtgcgc cagtaagaaa atacaatatg cgcccatcgt ctagcggtta
    11581 ggacatcgcc ctttcacggc ggtaaccggg gttcgattcc ccgtgggcgt
//
LOCUS       DAXHNE010000050        11404 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.050, whole genome shotgun sequence.
ACCESSION   DAXHNE010000050 DAXHNE010000000
VERSION     DAXHNE010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11404
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.050"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..79)
                     /locus_tag="ACFM4Z_001026"
     CDS             complement(<1..79)
                     /locus_tag="ACFM4Z_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HFC6021078.1"
                     /translation="MKPAVWAALLLCACTSNFGDREHQFL"
     gene            282..1610
                     /gene="miaB"
                     /locus_tag="ACFM4Z_001027"
     CDS             282..1610
                     /gene="miaB"
                     /locus_tag="ACFM4Z_001027"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFC6021079.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMVGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(1695..3602)
                     /gene="dxs"
                     /locus_tag="ACFM4Z_001028"
     CDS             complement(1695..3602)
                     /gene="dxs"
                     /locus_tag="ACFM4Z_001028"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFC6021080.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYNTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKESAAQMPSENKPAAKPTYTQVF
                     GKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLACE
                     GMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSFLR
                     CVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKGII
                     RREGGKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVTLE
                     ENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVERRV
                     RAWLSDRDAAN"
     gene            complement(3687..4604)
                     /gene="xerC"
                     /locus_tag="ACFM4Z_001029"
     CDS             complement(3687..4604)
                     /gene="xerC"
                     /locus_tag="ACFM4Z_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFC6021081.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDAGGVPQDLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCVWLVERGLLHTDP
                     TADIKPPKQPERVPKALSQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4798..5862
                     /gene="fba"
                     /locus_tag="ACFM4Z_001030"
     CDS             4798..5862
                     /gene="fba"
                     /locus_tag="ACFM4Z_001030"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFC6021082.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMANRYAKGELNQIVK"
     gene            6020..6841
                     /locus_tag="ACFM4Z_001031"
     CDS             6020..6841
                     /locus_tag="ACFM4Z_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H-binding protein"
                     /protein_id="HFC6021083.1"
                     /translation="MNRTAFCCLSLTAALILTACSSGGGGVAADIGAGLADALTAPLD
                     HKDKSLQSLTLDQSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIE
                     VDGQLITLESGEFQIYKQDHSAVVALQIEKINNPDKIDSLINQRSFLVSGLGGEHTAF
                     NQLPDGKAEYHGKAFSSDDAGGKLTYTIDFAAKQGHGKIEHLKTPEQNVELAAAELKA
                     DEKSHAVILGDTRYGSEEKGTYHLALFGDRAQEIAGSATVKIGEKVHEIGIAGKQ"
     gene            6980..7657
                     /gene="tsaB"
                     /locus_tag="ACFM4Z_001032"
     CDS             6980..7657
                     /gene="tsaB"
                     /locus_tag="ACFM4Z_001032"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFC6021084.1"
                     /translation="MQADFNRPVLAIDTGTSYLSLALRADGETHLFHQEAGSRQSELI
                     LPEIRTLFRNAGITAADLGAIVYTKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIRLPEGCAFSDG
                     IGSAFALEETPPFSGKPDMPTAADFLNLAAEGGFPAVAAAHSELLYVRNKIALTAKEQ
                     AERKART"
     gene            7654..8094
                     /gene="rimI"
                     /locus_tag="ACFM4Z_001033"
     CDS             7654..8094
                     /gene="rimI"
                     /locus_tag="ACFM4Z_001033"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFC6021085.1"
                     /translation="MNIRRASPSDCAALAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGGIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWFAHLPEDTQRLLLE
                     VRAGNTAAQALYAAHGFTDAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            8088..8825
                     /locus_tag="ACFM4Z_001034"
     CDS             8088..8825
                     /locus_tag="ACFM4Z_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC6021086.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVHTRKPAPETETPPPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFYGKAGVLLDNILKAVGLDAAYVHKTCWVKTAAVGNPMP
                     SEAAIETATVSVMQELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARAWQVLKQLKRALRQGGGS"
     gene            8887..9528
                     /gene="pyrE"
                     /locus_tag="ACFM4Z_001035"
     CDS             8887..9528
                     /gene="pyrE"
                     /locus_tag="ACFM4Z_001035"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFC6021087.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            9602..10036
                     /locus_tag="ACFM4Z_001036"
     CDS             9602..10036
                     /locus_tag="ACFM4Z_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HFC6021088.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            10033..11343
                     /gene="argA"
                     /locus_tag="ACFM4Z_001037"
     CDS             10033..11343
                     /gene="argA"
                     /locus_tag="ACFM4Z_001037"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFC6021089.1"
                     /translation="MSAPDLFVAHFREAVPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFYLDMLQTAASAAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAGGETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
BASE COUNT         2578 a         3628 c         2963 g         2235 t
ORIGIN      
        1 gcaggaactg atgttccctg tcgccaaaat tgctggtaca cgcacacagc agcaatgccg
       61 cccacacagc cggtttcata cacacctccc attaaagcca aacattatat agccgtcccg
      121 accgattaaa ttcatatttt aaaacaatat tctgtttaca aaacccacat cgtgctataa
      181 tccgcaccga ttttcagacg gcatcgtcat gccgtctgaa attttttcat tccaacaaca
      241 atcagccccg cgattacggc tgcctgagaa agacacaaac catgaaaaaa gtatttatcc
      301 gcaccttcgg ctgccagatg aacgaatacg acagcgacaa aatgctcgcc gtcctcgccg
      361 aagaacacgg cggcatcgaa caggttaccc aagccgacga agccgacatc atcttgttca
      421 acacctgctc cgtgcgcgaa aaagcgcaag aaaaagtctt ctccgatttg gggcgcgtgc
      481 gtccgctcaa agaaaaaaac cccggcctca tcatcggcgt tgccggctgc gtcgcctcgc
      541 aagaaggcga aaacatcatc aaacgcgcgc cttatgtgga cgtggttttc ggcccgcaaa
      601 cgctgcaccg cctgcccaaa atgattgtgg acaaagaaac cagcgggctg tcgcaagtcg
      661 atatttcctt ccccgaaatc gaaaaattcg accacctgcc gcccgcccgc gtcgaaggcg
      721 gcgcggcatt tgtatcgatt atggaaggct gttccaaata ctgctccttc tgcgtcgtcc
      781 cctacacgcg cggcgaagaa ttctcccgcc cgctcaacga cgtattgaca gaaatcgcca
      841 accttgccca gcaaggcgtg aaagaaatca acctcttggg acaaaacgtc aacgcctatc
      901 gcggcgaaat ggacgacggc gaaatctgcg acttcgccac cctgctgcgc atcgtccacg
      961 aaatccccgg catcgaacgt atgcgcttca ccaccagcca cccgcgcgag tttaccgact
     1021 cgattatcga gtgctaccgc gacctgccca aattggtttc ccacctgcac ctgccgattc
     1081 aaagcggttc cgaccgcgta ttgagcgcga tgaaacgcgg ctacaccgct ttggaataca
     1141 aatccatcat ccgcaaactg cgtgccatcc gtcctgattt atgcctgagc agcgatttca
     1201 tcgtcggctt ccccggcgag accgaacgcg agttcgagca aaccttgaaa ctggtgaaag
     1261 acatcgcctt cgacttgagc ttcgtcttta tttacagtcc gcgccccggc acgcctgccg
     1321 ccaacctgcc ggacgacacg ccgcacgaag aaaaagtgcg ccgcctcgaa gccttgaacg
     1381 aagtcatcga agccgaaacc gcgcgcatca accaaaccat ggtcggcacg gttcaacgct
     1441 gcctggtcga aggcatctcc aaaaaagacc ccgaccagct ccaagcccgc accgccaaca
     1501 accgcgtcgt caactttacc ggcacgcccg acatgattaa ccaaatgatc gatttggaaa
     1561 tcaccgaggc ctacaccttc tccctgcgcg gcaaagttgt cgaagcctaa acccttacgc
     1621 cgaaaaaatg ccgtctgaag cgtttcagac ggcattttgc cttgtatcgg cagacgatgg
     1681 cgcggccagg cggcttaatt tgccgcatcc cgatccgaca accacgcgcg cacacgccgt
     1741 tccaccgctt cggcactcaa gcccaaatcg tctaaaagtt ttttcggatc gccgtgtccg
     1801 gttacggtat cggcaacgcc caaaagcaaa acgggtttgc agatgccgtg tttcgccaat
     1861 acttccagca ccgcgccgcc tgcgccgccc tgttcggcgt tttcttcaag ggtaacgatg
     1921 cggtcgtggc ttcgggcaag gcggacaatc aactcttcgt ctatcggttt gacaaagcgc
     1981 atatcggcga cggtggcgtt cagtttttcg gcaaccgcca atgcgggggc gaccatactg
     2041 ccgaaggcaa tgaatgcggt tttcccacct tcgcggcgga taatgccctt gccgatttcc
     2101 acggtttcca tgccgtctga aaccggcgcg cccgtacccg tgccgcgcgg atagcggacg
     2161 gcggcgggcg cgtctgcctg atagcaggtc gaaagcaaca ggcggcattc gttttcatcg
     2221 ctcggcgcgg cgacaatcat gttcggcacg cagcgcaaaa agctcaaatc gtacagaccg
     2281 gcatgggtcg ggccgtccgc gccgacgatg cccgcgcggt cgacggcaaa caaaacgggt
     2341 aggttttgca gggcgatgtc gtgcaccagt tggtcgtagg cgcgttgtaa aaaggtggaa
     2401 taaatcgcca cgacgggctt catcccttcg caagccaaac cgccggcaaa ggtaacggcg
     2461 tgctgctcgg caatgccgac gtcgaaatag cggtcgggga atcgttgttc aaactcaacc
     2521 aagccgctgc cctcgcgcat ggcgggggta atcgcaacca gtcgggaatc tgccgccgcc
     2581 cggtcgcaca gccatttgcc gaacacttgg gtataggtcg gtttggcggc aggtttgttt
     2641 tcagacggca tttgcgccgc gctttctttg ggcaggttgg cgacggcgtg gtatttgacg
     2701 gggtcgtttt cggcaagttt gtagccgttg ccctttttgg tgatgacgtg cagcagttga
     2761 gggcctttgc gactgcgcaa gtctttcaat acgtccacca gattttcgac gttgtgtccg
     2821 tccacggggc cggtgtagcg gaagccgaag ttttcaaaca aagacagcga ctgtttggcg
     2881 tgttcggctt cttcggcaag ggttttgatt ttgtgttcga ctttttgggc aaactccatc
     2941 gcgccgggta ttttgtctaa taccttgccc gtttgcgctt tgacggtact caacaggccg
     3001 tgcatatcgc gcacgacgtt gctggcaagg tatttcggca gcgcgccgac gttgggggaa
     3061 atcgacattt cgttgtcgtt gaggacgacc agcaaatcca catccatatc gcctgcgcaa
     3121 ttcaaggctt caaacgcctg ccccgccgtc atcgcgccgt cgccgatgat ggcgacgctg
     3181 cggcggtcgc tgcccaagag tttgtctgcc gccgccatgc ccaacgccgc gccgatggag
     3241 gtggaggaat gccccacgcc gaacgcgtcg tactcggact cgcaacgttt cggaaaaccc
     3301 gccaaaccgc cgtattggcg catggtgtgc atctggtttt tgcgtccggt aaggattttg
     3361 tgcggatagc tttggtgtcc gacatcccac accagcttgt cttcgggcgt gttgtacaca
     3421 tagtgcaggg cgatggtcag ctcgaccgca cctaagttgc tggcgaaatg accgccggtt
     3481 tgaccgacgg attccagcag aaaggcgcgc aactcgccgg caaggcgcgg cagctgtttt
     3541 ttgtccagac ggcgcaaatc ttgcgggctg tcaatcaggt cgagtagggg gcttgggttc
     3601 atggtgtgtc ttttttatgt gtcgtccggg tgcaacggcc aattatatat caagagtgtg
     3661 cggctgacgg ctgattttgc cgtatgtcat tcgtcctgcc gcttggcgcg cgggtgggct
     3721 tcgtcataca ggcgggcgat gtggtcgaaa tcgagtttgg tgtaaatctg cgtggtcgaa
     3781 aggctgctgt gcccgagcag ctcctgcacc gccctgatgt cgcgcgaagc ctgcaacagg
     3841 tgtccggcgt agctgtggcg catcatatgc ggcgaaacgt gcctgccgtc gccgttttgc
     3901 gccgcccatt gcgccaaacg tttttggatt tggcgttggc tcaggcgcgt gccgttcctg
     3961 ccggtaaaca gggctttgcc gtccgatgcc gtctgacgca gcggcagata gtttttcagg
     4021 gcttccacgc ttttgccggt cagcggcacc tgccgctgct tgcgcccttt gccggtaacg
     4081 tgtacccacg cctcgtccaa atagacatca tctgcattca agccgtgtat ctcgctcacg
     4141 cgcaaaccgc tgccgtacat cagctcgaac agggcgtgat cgcgcaccgc cagcgggtcg
     4201 ccgccgtcca cgggcaaatc cagcatccgg ttcagccatt cctgcgacag ggctttgggt
     4261 acgcgctcgg gctgcttcgg cggtttgatg tcggcggtcg ggtcggtatg cagcagaccg
     4321 cgttccacca gccaaacaca atactgccgc caagacgaca gcttgcgcgc cagcgtccgc
     4381 gcattcaaac cgcgctgcga cagccgccgc aacgccgccg taaaatcgcg ccgcgacaag
     4441 tcctgcggca cgccgcctgc atcttcagac ggcatttgtg ccaacagcgc aaacagttct
     4501 tccaaatcgc gccggtatgc cgccaccgtg tgctccgact tgccctcgcg cacgatgttt
     4561 tccaaataag cgtcaaaatg cgccgcaaac ccgtccaaaa ccataaccgc cccacacaaa
     4621 tatcaaaaaa acagtgaaaa cattatcata aacgacacac caaaaccccg aaccgcccaa
     4681 aatccgaccc gtccgcccgc cgttatcgtt aacataaaat caatgaaccg tttatggttt
     4741 tttgccgcaa aaaacattat aatccgcctt atttacctat tgcccaagga gacacaaatg
     4801 gcactcgtat ccatgcgcca actgcttgat catgctgccg aaaacagcta cggcctgccc
     4861 gcgttcaacg tcaacaacct cgaacaaatg cgcgccatta tggaagccgc cgaccaagtc
     4921 aacgcgcccg tcatcgtaca ggcgagtgca ggtgcgcgca aatatgcggg cgcgccgttt
     4981 ttgcgccacc tgattctggc ggcagtcgaa gaatttccgc acatccccgt cgtgatgcac
     5041 caagaccacg gcgcatcgcc cgacgtgtgc caacgctcca tccaactggg cttttcctcc
     5101 gtgatgatgg acggctcgct gatggaagac ggcaaaaccc cttcttctta tgaatacaac
     5161 gtcaacgcca cccgtaccgt ggttaatttc tcccacgctt gcggcgtatc cgtcgaaggc
     5221 gaaatcggcg tgttgggcaa cctcgaaacc ggcgaagccg gcgaagaaga cggtgtaggc
     5281 gcagtgggca aactttcgca cgaccaaatg ctcaccagcg tcgaagatgc cgtgcgtttc
     5341 gttaaagata ccggcgttga cgcattggcg attgccgtcg gcaccagcca cggcgcgtac
     5401 aaattcaccc gtccgcccac aggcgacgtg ttgcgtatcg accgcatcaa agaaatccac
     5461 caagccctgc ccaatacaca catcgtgatg cacggctcca gctccgttcc gcaagaatgg
     5521 ctgaaagtca tcaacgaata cggcggcaat atcggcgaaa cctacggcgt gccggttgaa
     5581 gaaatcgtcg aaggcatcaa acacggcgtg cgtaaagtca acatcgatac cgacttgcgc
     5641 cttgcttcca ccggcgcggt acgccgctac cttgccgaaa acccgtccga cttcgatccg
     5701 cgcaaatatt tgagcaaaac cattgaagcg atgaagcaaa tctgcctcga ccgctacctc
     5761 gcgttcggtt gcgaaggtca ggcaggcaaa atcaaaccgg tttccttgga aaaaatggca
     5821 aaccgttatg ccaagggcga attgaaccaa atcgtcaaat aacaggttgc ttgtaaacaa
     5881 aatgccgtct gaaccgttgt tcggacggca tttgattttt gcttctttga cctgcctcat
     5941 tgatgcagta tgcaaaaaaa gataccataa ccaaaatgtt tatatattat ctattctgcg
     6001 tatgactagg agcaaacctg tgaaccgaac tgccttctgc tgcctttccc tgaccgccgc
     6061 cctgattctg accgcctgca gcagcggagg cggcggtgtc gccgccgaca tcggcgcggg
     6121 gcttgccgat gcactaaccg caccgctcga ccataaagac aaaagtttgc agtctttgac
     6181 gctggatcag tccgtcagga aaaacgagaa actgaagctg gcggcacaag gtgcggaaaa
     6241 aacttatgga aacggcgaca gcctcaatac gggcaaattg aagaacgaca aggtcagccg
     6301 cttcgacttt atccgtcaaa tcgaagtgga cgggcagctc attaccttgg agagcggaga
     6361 gttccaaata tacaaacagg accactccgc cgtcgttgcc ctacagattg aaaaaatcaa
     6421 caaccccgac aaaatcgaca gcctgataaa ccaacgctcc ttccttgtca gcggtttggg
     6481 cggagaacat accgccttca accaactgcc tgacggcaaa gccgagtatc acggcaaagc
     6541 attcagctcc gacgatgctg gcggaaaact gacctatacc atagatttcg ccgccaaaca
     6601 gggacacggc aaaatcgaac acctgaaaac acccgagcaa aatgtcgagc ttgccgccgc
     6661 cgaactcaaa gcagatgaaa aatcacacgc cgtcattttg ggcgacacgc gctacggcag
     6721 cgaagaaaaa ggcacttacc acctcgccct tttcggcgac cgcgcccaag aaatcgccgg
     6781 ctcggcaacc gtgaagatag gggaaaaggt tcacgaaatc ggcatcgccg gcaaacagta
     6841 gcatttgaaa aatgccgtcc gaacacgata atttaccgtt cggacggcat ttttgcgcca
     6901 taccgcccta ggatacgacg gcacggcagg caagccggtt tcactatata atgccgtctg
     6961 aaccaacgag aaccatacca tgcaagctga ttttaaccgt cccgtcctgg ccatcgatac
     7021 cggtacttcc tatttgtcgc tcgcgctgcg cgccgacggc gaaacccacc tgttccatca
     7081 agaggccggc agccgccaat ccgaactgat cctgccggaa atccgcactc tattccgaaa
     7141 tgcgggcatt accgccgccg atttgggggc gattgtttat acgaaaggtc ccggcgcgtt
     7201 taccggactg cgtatcggca tcggtgtggc gcaaggtttg gcaacgccgt ttgatacccc
     7261 tttaatcggc atccccacgc tcgatgccgc cgcctcgctg ccgccgccgc aaagctgcat
     7321 actcgccgct gcggacgcgc gtatgggcga agtgttttat gcgtggttcg acacgctgaa
     7381 ccgccgccgt ttgagcgatt atcaggtcgg gcgggcggca gacatccggc tgccggaggg
     7441 atgtgccttt tcagacggca taggcagcgc gttcgcgttg gaagaaacgc cgccgttttc
     7501 aggcaaaccc gatatgccga ccgccgccga cttcctcaat ctggcggcgg aaggcggttt
     7561 tcccgccgtc gctgctgcac actccgaact gctctacgtc cgcaacaaaa tcgccctaac
     7621 cgccaaagaa caggcggaac ggaaagcccg aacatgaaca tccgccgcgc ctcaccgtcc
     7681 gactgcgccg cactcgccgc gctcgatgcc gtctgcaacc cgtccgcgtg gacgcaacgc
     7741 caatttgagt ccgcattggt ttcgccgtcc gaacaggttt tcctcgcgga aaaagacggc
     7801 gggattgccg cctttatcgt ttggcagaac ctgcccgacg aatccgaact gcacctgatt
     7861 gccaccgcgc ccgaatgccg ccgccgtggc gttgcgtccg ccctgctcga atattggttc
     7921 gcacatctgc ccgaagacac gcaacgcctg ctgctcgaag tccgtgcagg caacaccgcc
     7981 gcacaggcac tgtacgcggc gcacggcttt accgatgcgg gcaggcggaa aaactattac
     8041 cgtacagccg acggtaaaac cgaagatgct gtcttaatgg agaaaatatg ttaagcgcgc
     8101 gctacctcca cctgcacgaa gctttgggtt taggcccgat gtggctgaaa caggccgccg
     8161 ccgtcctgcc gcccaaaaac acacccgcaa ccccggcaca ggcacgtcct caaaccgtcc
     8221 gcgccgcccc gatccgccct tcccaacccc ataacggtca ggcgcggctc gaaacgatga
     8281 aagcgttgga aaccgccgcc gtacatacgc gcaaacccgc gcctgaaacc gaaacgcccc
     8341 cgcccggcct ttcagacggc atcgcccccg ttcccgccgc ttcgggcata accaaacttg
     8401 ccgtcgtcag cctttgccca ccgattgagg atgcggttta cgggcaactg ttctacggca
     8461 aagcgggtgt tctgcttgac aacatactca aagccgtagg actggatgcc gcctatgtcc
     8521 acaaaacctg ttgggtgaaa accgccgccg tcggcaaccc gatgccgtct gaagcggcaa
     8581 tcgaaaccgc gacggtttcc gtcatgcagg aactcgacgg ctgccgcgcc cccgccgtcc
     8641 tattcctcgg acaggctttt gtccagcctg aacggcaaac gatgattgaa actttgtgcg
     8701 gcagccgtcc cttcttcatc atcgaccatc ccgcccggct gttgcgccaa cccgaactta
     8761 aagcccgcgc ctggcaggtg ttgaaacagt tgaaacgcgc cttgcggcaa ggcggcggca
     8821 gttgaagcgc accgcacggg gcggtagaat cgcaactgcg tcccaatatc tgacagaaag
     8881 cacaaaatga ccgatttccg ccaagatttc ctcaaattct ccctcgccca aaatgttttg
     8941 aaattcggcg aattcaccac caaagccgga cggcggtcgc cctatttctt caatgccggc
     9001 ctctttaacg acggcttgtc cacgctgcaa ctggcaaaat tttacgcaca atccatcatt
     9061 gaaagcggca tccgattcga tatgctgttc ggccccgcct acaaaggcat tattttggcg
     9121 gcggcaaccg cgatgatgct ggcggaaaaa ggcgtgaacg tcccgtttgc ctacaaccgc
     9181 aaagaagcca aagaccacgg cgaaggcggc gtgttggtcg gcgcgccgct taaagggcgc
     9241 gtgctgatta tcgacgacgt gatttccgcc ggcacatccg tacgcgaatc gatcaagctg
     9301 attgaagcgg agggcgcaac ccccgccggt gtcgccatcg cgctcgatcg catggaaaaa
     9361 ggcacgggtg agttgagcgc ggttcaggaa gtggaaaaac aatacggcct gcccgtcgcc
     9421 cccatcgcca gcctgaacga tttgtttatc ctgttgcaaa acaatcccga attcggacag
     9481 ttcctcgaac ccgtccgagc ctaccgtcgg cagtacggcg tagaataaaa acaaagcata
     9541 tgccgtctga accgccttac gcctcagacg gcatcaaacc tgacacacac gaggaaatac
     9601 catgcccgcc tgtttctgcc cccactgcaa aacccgtctc tgggtcaaag aaacccaact
     9661 caatgtcgcc caaggcttcg tcgtctgcca aaaatgcgaa ggactgttta aagccaaaga
     9721 ccatctggca agcacgaaag aacccatatt caacgatttg cccgaggctg tttcggatgt
     9781 caaactcgtt caccgtatcg gcacgcgcgc catcggcaag aaacagattt cccgtgacga
     9841 aatcgccggc atcctcaacg gcggtacaac ccagcccgat attccgcccg caaccgccgc
     9901 cacccctgct gccgcaccgc aggttaccgt accgcccgcc gcgcccgccc gtcaggatgg
     9961 gttcaactgg acgattgcaa ccctgtttgc ccttatcgtc ctcattatgc agctttccta
    10021 cctcgtcatc ctatgagcgc gcccgacctc tttgtcgccc acttccgcga agccgtcccc
    10081 tacatccgcc aaatgcgcgg caaaacgctg gtcgccggca tagacgaccg cctgctcgaa
    10141 ggtgatacct taaacaagct cgccgccgac atcgggcttt tgtcgcaact gggcatcagg
    10201 ctcgtcctca tccacggcgc gcgccacttc ctcgaccgcc acgccgccgc tcaaggccgc
    10261 acgccgcatt attgccgggg cttgcgcgtt accgacgaaa cctcgctcga acaggcgcag
    10321 cagtttgccg gcaccgtccg cagccgtttt gaagccgcat tgtgcggcag cgtttccggg
    10381 ttcgcgcgcg cgccttccgt cccgctcgta tcgggcaact tcctgaccgc ccgtccgata
    10441 ggtgtgattg acggaaccga tatggaatac gcgggcgtta tccgcaaaac cgacaccgcc
    10501 gccctccgtt tccaactcga cgcgggcaat atcgtctggc tgccgccgct cggacattcc
    10561 tacagcggca agaccttcta tctcgatatg cttcaaaccg ccgcctccgc cgccgtctcg
    10621 cttcaggccg aaaaactcgt ttacctgacc ctttcagacg gcatttcccg ccccgacggc
    10681 acgctcgccg aaaccctctc ggcacaggaa gcgcaatcgc tggcggaaca cgccggcggc
    10741 gaaacgcgac ggctgatttc gtccgccgtt gccgctctcg aaggcggcgt gcatcgcgtc
    10801 caaatcctca acggagccgc cgacggcagc ctgctgcaag aactcttcac ccgcaacggc
    10861 atcggcacgt ccattgccaa agaagccttc gtctccatcc ggcaggcgca cagcggcgac
    10921 atcccgcaca ttgccgccct catccgcccg ctggaagaac agggcatcct gctgcaccgc
    10981 agccgcgaat acctcgaaaa ccacatttcc gaattttcca tcctcgaaca cgacggcaac
    11041 ctgtacggtt gcgccgccct gaaaaccttt gccgaagccg attgcggcga aatcgcctgc
    11101 cttgccgtct cgccgcaggc acaggacggc ggctacggcg aacgcctgct tgcccacatt
    11161 atcgataagg cgcgcggcat aggcataagc aggctgttcg ccctctccac aaataccggc
    11221 gaatggtttg ccgaacgcgg ctttcagacg gcatcggaag acgagttgcc cgaaacgcgg
    11281 cgcaaagact accgcagcaa cggacggaac tcgcatattc tggtacgtcg cctgcaccgc
    11341 tgaccgcaac ggaaagccgc cgcagaaatg ccgtctgaac cccgtttcag acggcatttc
    11401 cccg
//
LOCUS       DAXHNE010000051        11311 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.051, whole genome shotgun sequence.
ACCESSION   DAXHNE010000051 DAXHNE010000000
VERSION     DAXHNE010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11311
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.051"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..338
                     /locus_tag="ACFM4Z_001038"
     CDS             <1..338
                     /locus_tag="ACFM4Z_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6021090.1"
                     /translation="FFDTEPDRGKIEHCACLAMIHEEISAMPMGYETLIGDMGSALSG
                     GQKQRIVLARALYCEPKILFLDEATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVE
                     SADRKMSLG"
     gene            complement(359..1585)
                     /locus_tag="ACFM4Z_001039"
     CDS             complement(359..1585)
                     /locus_tag="ACFM4Z_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HFC6021091.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            1740..1926
                     /locus_tag="ACFM4Z_001040"
                     /pseudo
     CDS             1740..1926
                     /locus_tag="ACFM4Z_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779592.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            2130..2789
                     /gene="queC"
                     /locus_tag="ACFM4Z_001041"
     CDS             2130..2789
                     /gene="queC"
                     /locus_tag="ACFM4Z_001041"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFC6021092.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQIYGRENVQAITFQYGQRHA
                     VELERASWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGALDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            2817..3335
                     /locus_tag="ACFM4Z_001042"
     CDS             2817..3335
                     /locus_tag="ACFM4Z_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HFC6021093.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTKMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            3342..3764
                     /gene="queD"
                     /locus_tag="ACFM4Z_001043"
     CDS             3342..3764
                     /gene="queD"
                     /locus_tag="ACFM4Z_001043"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFC6021094.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDDLVRGG
                     PKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            4033..4404
                     /locus_tag="ACFM4Z_001044"
     CDS             4033..4404
                     /locus_tag="ACFM4Z_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFC6021095.1"
                     /translation="MKLLSTLLVLLVAAEHFYIAWLEMTQIPSEKAAEIFKLPYEFME
                     QNRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            4401..5039
                     /locus_tag="ACFM4Z_001045"
     CDS             4401..5039
                     /locus_tag="ACFM4Z_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFC6021096.1"
                     /translation="MKKISVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGRLKAYAARNIIITGGEPTIQPHLDMLLDMLKAEGYFL
                     CIETNGLNPAPPQIDYVATSPKACYAAKYEKSCIETADEVRIVADGDVVAFCENMERK
                     IRAHHYYLSPCEQNGAMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            5254..5385
                     /locus_tag="ACFM4Z_001046"
     CDS             5254..5385
                     /locus_tag="ACFM4Z_001046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021097.1"
                     /translation="MAGLGKTKCRLKQHSVSDGIFLLPPKGKTAPAIYAEAVWIWSE"
     gene            complement(5374..5450)
                     /locus_tag="ACFM4Z_001047"
     tRNA            complement(5374..5450)
                     /locus_tag="ACFM4Z_001047"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5414..5416),aa:Pro,seq:cgg)
     gene            complement(5678..6763)
                     /gene="nagZ"
                     /locus_tag="ACFM4Z_001048"
     CDS             complement(5678..6763)
                     /gene="nagZ"
                     /locus_tag="ACFM4Z_001048"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFC6021098.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTGEERQRLLDPAVGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGQCAVIGNRSFHRNPEAVARLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDRRSLSELEAADLAPFHIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELRDDFRIPDNPALAQRWQYMANTLGSAAAQAVMQTAD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            complement(6820..8340)
                     /locus_tag="ACFM4Z_001049"
     CDS             complement(6820..8340)
                     /locus_tag="ACFM4Z_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFC6021099.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAVSDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            complement(8542..10041)
                     /locus_tag="ACFM4Z_001050"
     CDS             complement(8542..10041)
                     /locus_tag="ACFM4Z_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HFC6021100.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIKHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSSNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLIMRRGNTLFIALNLQ"
     gene            complement(10179..10808)
                     /gene="nth"
                     /locus_tag="ACFM4Z_001051"
     CDS             complement(10179..10808)
                     /gene="nth"
                     /locus_tag="ACFM4Z_001051"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFC6021101.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYKG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            complement(10854..11267)
                     /locus_tag="ACFM4Z_001052"
     CDS             complement(10854..11267)
                     /locus_tag="ACFM4Z_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFC6021102.1"
                     /translation="MMPAYAYAALAFSAFTSATLLPGTSEAAFALFVRRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLPFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
BASE COUNT         2644 a         3033 c         3162 g         2472 t
ORIGIN      
        1 gttttttcga tacggaaccg gatagaggaa aaatcgaaca ttgcgcctgc cttgctatga
       61 ttcacgaaga aatctccgcc atgcctatgg gctatgaaac cttaatcggc gatatgggca
      121 gcgcactgtc aggcggacaa aaacaacgca tcgtattggc gcgggcctta tattgcgaac
      181 cgaaaatcct atttttagat gaagcgacca gccatttgga tattgccaat gaaaaagcag
      241 tcaatgcaaa cttgaatggc ttgtctatca taaaaattat ggcggcacac agaaaggaaa
      301 cggtggaatc agcagatagg aaaatgtctt taggataaaa atacagtttc aaaaatactc
      361 aagattgctg ctgttttttc gcctgagcgt caaactctgc cagcgtcatg ttcaaagtct
      421 gcaaacacgg catcattacc gcatcgacag cttggttcac atgatccctt tccacaggca
      481 acggacggta aacgaagagc ttgaagagtt cgttcaactc aatcgaatcc gcccccgttt
      541 tcaacaccca accctgtctg ccggaataga tgtagccgtg ccgcgccagc ttttccaaaa
      601 gctcgcccaa ctcgtcgtag cccatattga tatgccgtct gaactcctga acaggcaagg
      661 ctttgccttc tttttgcgcc gcatccagaa gcagcaggat tttcaacacg tcgtcaaacc
      721 gtccgcgcga gtcgaagccc ctgcggaacg cttctccctg ccagtaggag agtgaagaag
      781 tcagcaccgc gccgcccaag accagcgtcc acaacaggtt cagccacaac agaaaaaacg
      841 gcacggcggc aaacgcgccg taaatcgagc ggtagccgtc gaaattgccc atataccaag
      901 tgaagaggga gcgcgcggtt tccagacaaa acgctgttgc caaagccccg acaaacgcct
      961 gccgcgcggg aacgaagcgg tttggcacga agcggtacag cccccacagc aaaagcgtca
     1021 tgaaggtcag cgtcgccgcc gttcgcaacg cgcccgacca ctgcggcgca cctgaggcaa
     1081 gcgcggcatc ctgtaccgag ccgaccataa aggaaatgcc cacgcccaaa gacagcggcc
     1141 cgaacgtcag taaagcccaa tagacgagaa actgcatcat ccacggacgc tgggaattga
     1201 cccgccagat gcggttgaac gtattgtcta tcgtccgaat cagcatcagc gaggtaacga
     1261 ccagcatcac gctgccgatt gccgtcagcc ggttcgcctg ctcgcggaac gcattgatat
     1321 agtcgaacac catgtccgcg ccctgcggca caatggtttg gttgacgaag gagacgaacg
     1381 aatccgacca gcggtcgaac acggggaaaa tcgaagcgac cgccaccatc acggtcagca
     1441 cggggacgag tgccagcagc gtcgtaaacg tcatgcttgc cgccgcctgc ggtacgcgtt
     1501 cttcatcaaa gcggcggacg acgaaccatg caaacgcaca gattttattg tctgccaaac
     1561 cttgcaaacg ttgtaaaaag gtcataattt cttgcccggt cagtaagttg ggcattgatg
     1621 cccgatgtta tagccaattt tgccgtcagg aacaaatgcc tgaactgcgg ctgtttcaga
     1681 cggcatcgga acaactgtta tgccgtctga agaccgaacc attttaacgg aatccgccca
     1741 tgaacccaaa tccccctcaa aatcctcgtc ctctactatt tccaaaacgg cagcacccgc
     1801 aatcccgcac tccgaatcgc tcgcggcatc gacagcgttg aaggttgcga agccgtattg
     1861 cgcaccgtcc ccaaagtctc cgccgtctgc gaagccgtca aaaaagatat tcccgacagc
     1921 ggctcccgtc ctgaccgccg aagaaaacaa tatcgccttc gcacaaagca aacgcttggc
     1981 ggaactcgcc gtcaagtcgg cataagccgc gtgttcagac ggcatggcgt tcagatgccg
     2041 tctgaacgcg tttgcctgta taatccgcgt ctttactgtc caacttcgcg gttcgcaaac
     2101 ctcccgcgtt accaaaacta ggattcgata tgtcaaacca aaaagccttg gtcatctttt
     2161 cgggcggtca ggattcgacc acctgcctga ttcaggcaat ccaaatctac gggcgcgaaa
     2221 acgtccaagc cattactttc caatacgggc aacgccatgc cgtcgagctg gaacgtgcca
     2281 gctggattgc gcaggatttg ggcgtcaaac aaaccgtact cgacttgagc ctgatgcggc
     2341 agattacgca caatgccctg atggacgaca ccgccgccat cgaaactgcc gaaaacggcg
     2401 ttccgaatac ctttgtagac ggccgcaacg cgcttttcct gctctatgcc gcgatttacg
     2461 ccaaagggca ggggatacgg cacatcatcg cgggcgtgtg cgaaaccgac ttctccggct
     2521 atcctgactg ccgcgacgtg ttcgtcaaat ccatgaacgt tacccttaat ctggcgatgg
     2581 actatgattt ccaaatccac acgccgctga tgtatctgac caaggcgcaa acttgggcgt
     2641 tggcggacga aatgggcgcg ctggactata tccgcgagca aacccacacc tgctataacg
     2701 gcatcgtcgg cggctgccgc gaatgcccga gctgtatctt gcgcgaacgc gggctggcgg
     2761 aatatctgga aagtaaaaag gccgtctgaa cacgcgcaaa ccataaggaa tacgatatgc
     2821 ccaagctcca tatgttttac ctcggcggca atgccggcag gtcgaatatc gaagtgcacg
     2881 acatccaatt tgccgtgtgc gacgactacc gtgaagccgt ccccgcactc aaagccgcgt
     2941 ggttcggcga tgcggacaaa atccacatcg acggctggca ggttgtcgaa tgggcggacg
     3001 gttacgacat cgccgtatcc gaaacgccca aaacgaaaat gccgtctgaa cacgccccgc
     3061 gcctgtattt cgccaatgtc ggcggttatc gcgcagggca gctcgccgaa gcgcacgctt
     3121 ttgggctgtt cgccgccgcc acgcctgccg aagccaaaca aaaagccctg caaaccctgt
     3181 tgaccgacag ctatgttcag caacataaag acaacttaaa agacgtggac aacctgcttg
     3241 cgctcgaccg catcggcaat ttccatatcc gcctgacccc gaatccgcac ggcaaacccg
     3301 ccgaaatcgg ctttcaaggc tatttgccca tttgagaacc catgaaaatc accaaaatct
     3361 tcaccttcga ctcctcgcat atgctcgacg ggcatgacgg caaatgccaa aacctgcacg
     3421 ggcataccta caaactcgaa atcaccgttt cagacgacct cgtcagaggc gggccgaaag
     3481 acggtatggt gatggacttt accgacttga aagccattgt caaacaacac attaccgacc
     3541 ccttcgacca cgccttcatc taccacggcg gcaacgggcg cgaatctcaa atcgccgcac
     3601 tcttggaagg ctggaacatg aaaaccctgc gcctgccctg ccgcaccact gccgaaaata
     3661 tggcggtcga aatgtacgac cgtctgaaaa acgcggggct gaacgtgtgc cgcgtcaaat
     3721 tgtgggaaac gccgacatcg tgtgcggagt atgaagggga gtagggaata tcttgaacgt
     3781 atcgatatag taaattccaa taagacatgc ccaaccgcgt cattcccgcg caggcgggaa
     3841 tccagacctt ggtttttcag gaatatttaa aaattgcagc aattccaaat ctttggattc
     3901 ccgcctgcgc ggaaaggacg gtttagagcg tccttatttg aatttaccgt aaaacggttt
     3961 tttctcctgt acggattccc cgttttttca gacgaccttc catatcaaat acacccatta
     4021 aaaggaatac ccatgaaact tctctccacc ctcttagtcc tccttgtcgc cgccgaacat
     4081 ttctacatcg cctggcttga aatgacgcag attcccagcg aaaaagcggc ggaaatattc
     4141 aagctgcctt atgaatttat ggaacaaaat cgcgtgcaga ccctgttcgg caaccaaggg
     4201 ctgtataacg gctttctcgg catcgggctg gtgtggtcgc ggtttgccgc gccggataac
     4261 gcggtgtacg gcgcgacggt actgtttctt ggcttcgtcc tgattgccgc cgcgtggggc
     4321 gcgttctctt ccggcaacaa aggcatactc gtcaaacaag gtttgcccgc atttttggca
     4381 gcggcggcgg tgttggcggt atgaaaaaaa tcagcgtcgc ccccgaaaat ccccaatacc
     4441 gcatcgtcga aatctttgaa agcctgcaag gcgagggctg gaacacgggc atgcccgccg
     4501 tgttcgtccg cctgggcaaa tgcaacctcg cctgcggctg gtgtgatacc gattatttga
     4561 cattcggcat gatgagcctg tccgacatct taggccgtct gaaagcctat gccgcccgca
     4621 acatcatcat caccggcggc gaaccgacca tccagccgca tctcgatatg ctgctggaca
     4681 tgctcaaggc ggaaggttat ttcctctgta tcgaaaccaa cggactcaat cccgcgccgc
     4741 cgcaaatcga ctatgtcgcc accagcccca aagcctgcta tgccgccaaa tacgaaaaaa
     4801 gctgtatcga aacagccgac gaagtgcgga ttgttgccga cggcgatgtc gttgcgttct
     4861 gtgaaaatat ggaacgcaaa atccgtgcgc atcattacta cctttcgccc tgcgaacaaa
     4921 acggtgcaat gaacatctac gacaccatcc gccaaatcgg tattctgaac agccgccccg
     4981 acgcgccggt gcattggcag ttgagcgtgc agacgcacaa atgggcggga atagagtagt
     5041 ttaagcagtg taactcaaag ggacggcgta cggttttacc gatgtttgac atacggggaa
     5101 agtgtgccgc ttctgcgtgg aaatgccggc atttccaccg cccaatcagg acggagcctt
     5161 actgaataag atgctgccgt tgggtacaag ctcggcttcc taaattccga tggtcttttg
     5221 aaccttgccg atactctgtg ccagtgcgcg caaatggcag ggttagggaa aacgaaatgc
     5281 cgtctgaaac agcattctgt ttcagacggc atttttctgt tgccgccaaa aggaaaaacc
     5341 gctccggcaa tctatgccga ggcggtttgg atttggtcgg aatgagagga ttcgaacctc
     5401 cgaccccttc gtcccgaacg aagtgcgcta ccgggctgcg ctacattccg aattaagtaa
     5461 ggcgtgatta tagcgcaaaa aatacggcgt gcctataccg ttttgccttt ttgccgcgtg
     5521 tcgggcggat ttaaaggagt gtgtttgaat atagtgttga taatcatcat tatctttaag
     5581 taattcaata agataacttt ctacctgacc gaaaaaatca ttgcctttcc ctgacaaacg
     5641 gttgatgaaa tcggcagatt gttgaaacgc agccggttta aaaggcttcg ccgactttta
     5701 cgccgcccgc cgtgtcttgc ggcgaggcaa ggccggcaac aaaggcttgc gccgcttgga
     5761 aatccgccgt ctgcataacg gcttgcgcgg cggcactgcc gagcgtgttg gccatgtatt
     5821 gccaacgttg cgccaaagcg ggattgtcag gaatgcggaa atcatcgcgc agttcgtcca
     5881 caaggtcggg acggttgcag acgaggacga tgtcgcaacc tgcctcaaag gaaatgcggg
     5941 cgcgttcttt gatgccgcct gccccgcacg cgccctccat agttaaatcg tccgagaaaa
     6001 tcacgccttt gaacccgatg tcgcggcgca aaatttgttt gagccagatt tcggaaaacc
     6061 ctgcgggctt tgtgtccact tgtggataaa cgacgtgggc gggcataacc gccgccatac
     6121 cttcgcggct cataatgtgg aagggggcga ggtcggcggc ttcgagttcg gacaggctgc
     6181 gcctgtcttc cggcaagacc agatggctgt ctccttcgac aaatccgtgt ccgggaaaat
     6241 gtttgccgca ggatttcata ccgccttttg ccaaaccttt ttgaagggcg agggcgaggc
     6301 gtgcgaccgc ttcggggttg cggtggaagc tgcggttgcc gatgacggcg cattgtcccc
     6361 agtccaagtc gagaacgggg gtgaaggaga ggtctatgcc gcaggcggaa agctcggttg
     6421 ccaacacccg gccgacttgt ccggcggcgg tttcggcggc ggacgcgccg tctttgtccc
     6481 aaatttgccc taaaacgttc attgcgggca ggcgggtgaa gccttcgatg aaacgttgca
     6541 ccctgccgcc ttcgtgatcg acggcgataa tgagttcggg tgtgcgcagg gctttgattt
     6601 cggcggtgag tgttttgagt tgttcgatgt tttggaagtt gcggcggaag aggatgatgc
     6661 cgcctacggc gggatcgagc aggcgttgcc tttcttctcc ggtcaggcgg aaggcggcaa
     6721 tgtctgccat gacggggccg cgcggaatat gggggacggt cattgcggtt tgctccaaaa
     6781 agcttcagac ggcatatgcc gtctgaacag ggaaaggggt caggcgttgg cgcgtttttt
     6841 atctttcaac agaaaaatca gcaccgccaa tacaatgcct gtcgtgccaa agcccaacag
     6901 cgcggatttt gtcagaccca atgcgaggta gcccgatgcg gcggcggcgg caacggttaa
     6961 ggcgtaaggc agttgcgagg taacgtggtc gatgtggttg cagcgcgcgc cggtggacga
     7021 caggatggtc gtgtcggaaa tgggcgagca gtggtcgccg cataccgccc ccgccattac
     7081 tgcggacata cacgggataa tcagcgcggg ttcgactttg accgccatgg cggcggcaat
     7141 cggcagcata atgccgaacg tcccccagct tgtgcctgtg gcaaacgcca tcacgctggc
     7201 gagcaggaag aggatgacgg gcaggaagcc gggatggatg ttgcccgcaa ccagtgtgga
     7261 gaggtaatcg ccggtgtgca tttcgccgac aaccgtactg atgagccaag cgaggattaa
     7321 aatggcgatt gcgccgaaca tagatttcgc accctgccaa acggctttgg gatagtcggc
     7381 ggttttaatc gtgccgagcg tgcagagaac gacggcaagg acgccgcaag tgccgccgaa
     7441 taccagcgaa gtgtttacgt ccgtgttttc aaatgccccc aaaatgctga aggtttcgct
     7501 tgcctgcgcg ccggtgtaga tcatggcgga aaccgttgag gcgattaagg ccaaaacggg
     7561 aataatcagt gcgtaaacac gacctttggt agcgtctgaa acggcagttt catcgtgggc
     7621 ttcgttcaac gcggcttgtt cgaaacgtgc catcgagccg atgtcgaagg aaaaccatgc
     7681 gacgacgaac accataatca gggcaaacag tgcgtaatag ttcatcaggc tcatggcgac
     7741 aaacgtcccc atcggcgtgt attcggtgat tttgtaggta acgagcagtc cggcaagcgt
     7801 ggcgataatc gacgcgcccc agcttgaaac gggcatcagc acgcacatag gcgcggcagt
     7861 ggagtcgagg atgtaggcga gtttggcgcg ggaaacttta aacttgtcgg taacggggcg
     7921 ggcaatcgca ccgacggcga gactgtggaa atagtcgtcg ataaaggtta cgaacacgag
     7981 gcaggcggtc agcattttcg cgccgcgccg gtttttaatg tgccgttttg cccagtcggc
     8041 aaacgcctga ttgctgccgg agtaggtcag cagggaagta aaaataccca aaagtatcag
     8101 gaaaaccaag atttttggtt tgcccagcga ccaatcgccg tctgaccaag ccaagccgac
     8161 gaccatgtct ttcaggtgtg tcagaccgtc gacggggttg ccgccgacca aaaaggcaac
     8221 gccgaccaga ataccgatgc ctaaagacag cagtacgcgg cgggtaatga cggcaagtgc
     8281 cagtgccaaa aagggtggca caaccgagaa aaatgaatgt gaatagtcga tcagctgcat
     8341 ggttatgggg gtgttaagcg tccggatggg agcgtatctg tccgcctccg gtttgggttt
     8401 tgttggcaaa atgggcggaa atattttttg tcgtaaaaaa tatttgttta aaatcaacca
     8461 actgattttt gtaaaatgcc cgttaatcgg tattgacggg cattttatca tttaaaaaat
     8521 attttggtta aattatgtgt gttattgcag gtttaatgcg ataaacagcg tgttgccacg
     8581 gcgcatgatc agcaggggga cgtttttgcc tgccttgtcc atagctttgc ggaaaccggc
     8641 ttcgtcattg acggggactt gcccgacggc aagaatttcg tcgccgcgcc tcaagcctgc
     8701 gcgttctgcc gcgtcggaaa cccgtacgac gacgaggtgt ccgccgctgc tgtcggtatg
     8761 tgtctgaagg gtaatgcctg cggattcgac cgagaacgta ccggattgct gttcggtgta
     8821 gggggcttca tctgttttgg atgatgcgcc gatatgctcg gcggcgttgc ccagcttgac
     8881 tttgattgtg atttcttcgc ctttgcgcca tacgccgagg ctgacttctt ttcccggcgt
     8941 aatggcgccg accataacgg gaaggtcgcc ggaagaacgt atttctccgc cgtcgaggct
     9001 gaggacgatg tcgcccgcct gcaggccggc acgttctgcg gggctgccgg gcaggatttt
     9061 ggcaatcagt gcgccgccgg ctttgtccaa accgaacgat tgtgccaaac cgtaggatac
     9121 ttcttgaata atcacgccca gttgtccgcg ttggactttg ccggtgtttt tcagctgttc
     9181 ggcgacattc atggcaacgt caatcgggat ggcgaaggaa atgcccatga atccgccgct
     9241 gcggctgtat atttgcgagt tgatgccgac gacctgtcct tttaagttga acagcgggcc
     9301 gccggagttg cccggattga tggcaacgtc ggtttggatg aaaggtgtgt agctttcgtt
     9361 gggcaggctt ctgcctttgg cggacacgat gccggcggtc acgctgttgt cgaagccgaa
     9421 gggcgcgccg atggcagcga cccattcgcc tggtttcaaa tctttgggat tgccgatttt
     9481 gacgacgggc agctcttccg ttgcgtcgat tttcagaagg gcgacatcgg attggacatc
     9541 cgaaccgatg agtttggcgg tatattcgcg cttgtcgttg agcaggactt tgatactgcc
     9601 catgccggta acgacgtgcg tattggtcag aatatagccg tctttgctga tgatgaagcc
     9661 cgaaccgaag ttcaatccgc cgtcatctgc ttcttcttgg gggatttcgg gcatgttcgg
     9721 gacgaggcgt ttgaaaaatt cgtagaacgg gtcgctgtcg gcaagcgggt cggaatcggt
     9781 ttcggcattg ctgctgccgt tttgggtgcg cggggcgggg gctgcctgaa tattgacgac
     9841 tgccgggcct tcgctttgaa ccagttggac aaagtcgggc agcagcatac tgacgctgcc
     9901 gtcgtctttg gtgtgtttga tgcgttctac gaaggatgct tcttttttgt ccgcaccgaa
     9961 aaagctgccg gctttgtcgc agcctgccag cgaggcggca cacagtgctg ccaaagcgag
    10021 gtattggtat tttttgaaca cgttttgtcc tttgtcggat gccggtaccg gctttaatgc
    10081 cgtctgaagc gcattttgtc ggcttcagac ggcataggtt gaaattctac aacgtccgtc
    10141 cgaattttca agcgtttcat tttgaagggc ggcggcagtc aggctttggc gggatattcg
    10201 cacaaatcgt tgatgatgca ggtttggcat tgcggtttga gtgccttgca ggtgtagcgt
    10261 ccgtgcaaaa tcagccagtg gtgcgcgtcc atcagaaatt ctttaggaat gaagcgcatc
    10321 agtttgtctt cgacttcgcg cacatctttc ccgggtgcga ttttggttcg gttggacacg
    10381 cggaaaatat gcgtatcgac cgccatgacg gggtgtccga acgctgtgtt caataccacg
    10441 tttgccgttt tgcgccccac gcccggcaac gattccaaag cctcgcggtc ttccggcact
    10501 tcgcccttgt atttttccag caggatgcgg caggtctgca tgatgtgctt ggacttggtt
    10561 ttatacaacc cgatggtttt cgtgtattcc atcacgccgt ccaagcccaa atccagcatt
    10621 gcctgcggcg tatcggcaac cggaaacagc ttcgccgtcg ccttgtttac gccgacatcg
    10681 gtcgcctgcg ctgaaagcag aacggcgatt aaaagctcga aaggggagtt gaaattcagc
    10741 tcggtggtcg gatgggggtt ggcggcgcgg aagcgttcga aaatttcttg gcggatgtgt
    10801 ctgttcattt ttttgtgcag tcggtttgtg cggtcggcat tataacgcac ggttcaggcg
    10861 gcgtaatatt gcattcccca cagaatgaag gcgtaacgcg ccgttttgcc gataaccagc
    10921 atcagcccgc ttgtccacgg attcaaccgc agccagccgg cggcaagcgg cagtgcgtcg
    10981 ccgacgacgg gcagccaggc aaacggaagc agccaaatac cgaaacgccg catcagattc
    11041 agtgtttttt cagacggcat ttttcgggaa ggcagcaaac gtcccatcca ataggaaacc
    11101 atactgccca atccgttggc aaggccggcg cacagcaacg cgccgtatgc gtgttcggga
    11161 aagcggcgga cgaacagggc aaaggcggct tcggatgtgc cgggcaggag ggtggcggaa
    11221 gtgaaggcgg aaaaggcgag cgcggcgtag gcgtaggcgg gcatcattgc aaacagtctc
    11281 aaacaggcaa aaatcggcga cggattgtac g
//
LOCUS       DAXHNE010000052        11234 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.052, whole genome shotgun sequence.
ACCESSION   DAXHNE010000052 DAXHNE010000000
VERSION     DAXHNE010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11234
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.052"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            52..1005
                     /locus_tag="ACFM4Z_001053"
     CDS             52..1005
                     /locus_tag="ACFM4Z_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFC6021103.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1110..1433)
                     /locus_tag="ACFM4Z_001054"
     CDS             complement(1110..1433)
                     /locus_tag="ACFM4Z_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021104.1"
                     /translation="MKKCILGILTACAAMPAFADRISDLEARLAQLEHRVAVLESGGN
                     TVEIDLFGSNSTMYVCSITPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1606..2967)
                     /locus_tag="ACFM4Z_001055"
     CDS             complement(1606..2967)
                     /locus_tag="ACFM4Z_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFC6021105.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTIIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEVIEVTTFRGGAKVHQNARGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGMDDIAERKLVMIGNAAERYQEDPVRILRAIRLSGKLGFELSQATAGPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPAPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRTEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
     gene            complement(3399..5099)
                     /gene="recJ"
                     /locus_tag="ACFM4Z_001056"
     CDS             complement(3399..5099)
                     /gene="recJ"
                     /locus_tag="ACFM4Z_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFC6021106.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAARRLADAVERQEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAEQGVDLLITVDNGIASIAGVARAQELGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLMALRAELRRRNYFSDGIKEP
                     NLGELLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDTEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQTTLAVYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVREDDLSQTFITDGSLKACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKAWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAA
                     "
     gene            complement(5168..5881)
                     /locus_tag="ACFM4Z_001057"
     CDS             complement(5168..5881)
                     /locus_tag="ACFM4Z_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021107.1"
                     /translation="MLFRKTTAAVLAATLMLNGCTAMMWGMNDPFSQTTAYKHVDKDQ
                     IRAFGVVAKDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKQFQMVEPNPR
                     FAYQALPVKLESPASQNFSTDDLCLRYDTDRPADIAKLKQLEFEAVELDNRTIYTRCV
                     SAKGKYYATPQKLNADYHFEQSVPADIYYTVTKKHTDKSKLFENIAYTPTTLILDAAG
                     AVLVLPMAALIAAANSSDK"
     gene            complement(5928..6434)
                     /gene="yjgA"
                     /locus_tag="ACFM4Z_001058"
     CDS             complement(5928..6434)
                     /gene="yjgA"
                     /locus_tag="ACFM4Z_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFC6021108.1"
                     /translation="MFEQEDEWISKTQMKKQMNDLQDLGMALTKLSNDTLKKIGLDAD
                     LYEAVTAYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     QARVRLLADDGALTQFMSDFPHADAGKLRTLVRNTKKEQEQNKPPKNFRALFQELKTV
                     MENGDAEI"
     gene            complement(6466..6597)
                     /locus_tag="ACFM4Z_001059"
     CDS             complement(6466..6597)
                     /locus_tag="ACFM4Z_001059"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021109.1"
                     /translation="MNSIEIFLDDALRHFNRFGRQGQKRAVCRADGAKCRLNAAAFC"
     gene            6587..7918
                     /gene="pmbA"
                     /locus_tag="ACFM4Z_001060"
     CDS             6587..7918
                     /gene="pmbA"
                     /locus_tag="ACFM4Z_001060"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFC6021110.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKATGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRASTADFSEKALQDTVKAAIDIARHTAEDGCA
                     GLADACLMAKHIGDPDLYHEWDLDTEAAVGLAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(8215..8418)
                     /locus_tag="ACFM4Z_001061"
     CDS             complement(8215..8418)
                     /locus_tag="ACFM4Z_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFC6021111.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            8661..8963
                     /gene="clpS"
                     /locus_tag="ACFM4Z_001062"
     CDS             8661..8963
                     /gene="clpS"
                     /locus_tag="ACFM4Z_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFC6021112.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            8965..>11234
                     /gene="clpA"
                     /locus_tag="ACFM4Z_001063"
     CDS             8965..>11234
                     /gene="clpA"
                     /locus_tag="ACFM4Z_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFC6021113.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDDGNYSSDGMD
                     DDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSGPTGVGKTE
                     AAKQLAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGF
                     TAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHQLLDKK
                     VEAEFTPALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSDGGFVRIDW
                     DAAKEEAVLKFKKSKV"
BASE COUNT         2671 a         3275 c         2827 g         2461 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgc
      361 accaagcgcg gcagcatcgg cggcagaacg ccacggcaga acggctatat ccgcgcccta
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatctcgcc
      481 gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgg
      541 cctgccgtcg aagccggcga gaaactgggt tttctgcccg gtgacctgac ccagaaagtc
      601 gatccctacc ttcgtccgct ttatgatgcc ctctatgacc tgatgggctt cgaccgtgtc
      661 accaagctga ttgaaaaagg tctgattgag attgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 ggcgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctttg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa atcgggcaga gaaaaacgga tacggaagcc aagctccgta
     1081 tccgtttctt tatccctcca accgatacat caatcgaatt ttctgcatcg gattgcctca
     1141 tcttcgcaaa acattgccga agtttcgcgg ttgcacgcct gacgcacttt ctgccgcgcc
     1201 acgccttcat tccggtcgct tgcctcaaac gtcttctgaa aaggcgtaat gctgcataca
     1261 tacatggtgg aatttgaacc gaaaaggtcg atttcgacgg tattgccgcc gctttccaat
     1321 acggcgacac ggtgttccaa ctgcgccaga cgtgcttcca aatcgctgat tctgtcggca
     1381 aatgcaggca tggcggcaca cgcggtcaaa atgcccaaaa tacatttttt catcaatttt
     1441 tccttgttgc ttgaaccctg cccacccgga catccttttt ccgagaggca gggtcagact
     1501 ttatttacgg ggatgaagcc ccttacaaat caatataata catggaacgg cactttatgc
     1561 ttcatcccat gtattgaaac ttcattgctc cttgtttgtt gaccgttatt cccaatccgt
     1621 tcccacaacc ttcggcttgg gtttgcgcct gcgacggcgg cgttttttcg cctgtccttc
     1681 gtttttttcg tgccgggcgg cagcctcgtt tttggtcatt tccgtccgct gttccgtcga
     1741 tgccgtctga aacgccgtcc accactcggc aagggcgcgg tccgcattgc cggtttcggc
     1801 gcgcaagagc aggaaatcat aggcggcacg gaaacgcgcc tgtgcaaaca gtttgtgcgg
     1861 acgtgcgcct ttgcggtttt caaactgcgg ctgaaacatc caaatttcgc gcatcgtggc
     1921 ggaaaagcgt tgcggcacgc cccaaccgcg ttcgacggtt tcacgcatcg tattgattgc
     1981 atcggacagg gcgggcgcgg gtttcaaacc ctgttgcaga ttgcttttcc aatggcgttc
     2041 caactcgggc cacatcagag ccgccagtac aaaaccgacc gaaaccgatt tgtcggcacg
     2101 cagccgctca tcggtatttt tcagggcaag caccgtcatt tttccggcga tgccgtctga
     2161 aacgcgcaag gcattgagca gcggatggat gtcgtccggt atgtcaaatc cgttcaaacg
     2221 tttcagacac tcgcgcgcgt gccctgaaaa cagcagtttc atgatttcgt caaacagcct
     2281 cgctaccggt tcgtgcttca gacggcatat tgattcggca ataggtccgg cggtcgcttg
     2341 cgacaattcg aatcccaatt tccccgacag gcggattgcc cttaaaatcc tgacagggtc
     2401 ttcctgatac cgttcggcgg cattaccgat cattaccagc ttccgttcgg caatatcgtc
     2461 cataccgttg tggaaatcca aaatttcttc tttttccgga tcgtaataca acgcgttgca
     2521 ggtaaagtcg cgcctcatgg catcttcttc gatactgccg taggtattgt ctttcataat
     2581 cctgccgcgt gcattctgat gtactttcgc accgccccga aaagtcgtta cctcaatcac
     2641 ttccgcaccg ttcatcacat gaacgatttg gaaacgcctg ccgatgatgc ggctgcggcg
     2701 gaagagtttg tgcacctgtt cgggcgtggc atcggttgcg acatcgaaat ccttgggttc
     2761 gatgccgagc agcaggtccc tgaccgcgcc gccgaccaca taagcctgaa accccgcccc
     2821 tttcaggcgg cgtatgacgt tttcggcggc aaagctcaac atttcggcac ggatgttgtg
     2881 tctttcggca ggaatgatcg ttttactttc cgctttttta ctgctccgac cggaaggcag
     2941 catcttattt aaccattttt tcagcataat attctaaccc gaagagcggc atctccacgg
     3001 gcaaccgtgt tcagacggca tcgggcgcgg tttgcaagat gccgtaacca taaacaaaag
     3061 gcgtagaaac tacgccgtcc gaatcacgcc gccctcgcgg cggcaacgcg tcattataac
     3121 agattgccct ccaaaaaaat cagaccctgc ttttgtggca gagtctgaaa tttgatgtgc
     3181 cttctccgat tgggaacatt cccgtcgtac aaacaggcaa atgtttcaac acacaggacg
     3241 acacataaag caccgtccta tgtgttgccc tgatttggaa ggggttacgc tccctcccaa
     3301 ataaggtctg attctaccgc cctaaagggt ggggtttcaa ccgaaaagga aatacgatga
     3361 aggaacagaa atcacattca aacggcgttc tgccgcctct acgccgcttc ccagtagtcg
     3421 atataaagct gcaattcgag attgttgcgc cattcgttgg caacggggcg gtaaaccgtg
     3481 cggatgtatt cggggatgtc ttcgctgcaa cgccaaaaca tggcttcaaa ttcgcagccg
     3541 tctttctgca gccacgcttt tttgtgttta ccctccgcgc ccaaaggctg ctggcggacg
     3601 acgtggaact cgtcggtaaa gcttggcggc gcgaagccct gcccccaaac gtgtcgggca
     3661 aggttttgag cctgttccag cgtgatgtcg caggctttca ggctgccgtc ggtgatgaag
     3721 gtttgcgaca aatcgtcttc gcgcaccatc tcgcgcacgg cttcttcaaa ggccgtctga
     3781 aacgcgggaa tattgtgttc aagtatgctc aagcccgccg ccattgcgtg tccgccgaat
     3841 ttcaaaatca aatcgggatg gcgtttggac accaagtcca aagcatcgcg caggtgcaaa
     3901 ttgggaatgg agcgtcccga accgcgcact tcgccgttgt ccgccggagc aaacacgatg
     3961 gtcggacgat aaaaacggtc tttgaggcgg ctggcaacga tgccgaccac gccttgatgg
     4021 aagtcgtcgc gatacaccgc caaagtcgtc tgacctgaag gcagggtttc aggaaaagca
     4081 ttcagcgcgt cttgcagcat agactgctcg atttcgcgac gctcgatatt gaggttgttt
     4141 aactgcgccg ccagttcctg cgcctcggta tcatctcgcg ccaacaggca ggcaataccg
     4201 accgacatat cgtccagccg tccggcggcg ttgatgcgcg ggcccaacgc aaaacccata
     4261 tcaaacggct gcgccttgcg ccaatcgcgc cgcgccactt caaacaaggc gcggataccg
     4321 gggcgcattt tgccggagcg catccgtttc aaaccttgcg acacgaggat gcggttgttg
     4381 tggtcgagag ggacgacatc ggcaaccgtg ccgagtgcga ccaaatccaa aagttcgccc
     4441 agattcggct ctttgatgcc gtctgaaaaa taattgcggc ggcgcaattc ggcacgcaac
     4501 gccatcaata cataaaaaat cacgcccacg cccgccaagc ttttgcttgg aaaaccgcag
     4561 cctttttggt tcggattgac gatgatgcag tcgggcacgg tctcggcagg cagatggtgg
     4621 tcggtaacga tgacatccaa acccaattcc tgcgccctcg ccacgcctgc gatgctggcg
     4681 ataccgttat caaccgtaat cagcaaatcc acaccttgct ctgccgcgat ttcagccagc
     4741 tcgggcgtta agccgtagcc gtgttcaaag cggttgggca cgaggaaatc cactttcgcg
     4801 cccatcgccg ccaaaccgtc caaaccgacg gcacacgccg tcgcgccgtc ggcatcatag
     4861 tcggcgacaa tcaggatttt ttcctgacgc tcaaccgcat ccgccaaacg gcgggcggcg
     4921 gcttcgcaat tcgtcagcga ttgataaggc aggagggaag cgagtttgtc gtccaattcg
     4981 gcaggacttt gcacaccgcg cgaagcacaa agccgggcga ttaaaggatc ggcaccggcg
     5041 gtgagcaaat gattaaaaac gtcggtattg acggagcggg tttggatttt gacggacata
     5101 acgtggaact ttacagtgcg ggtgtgcttg aaatgccgtc tgaaggtttt gcagacggca
     5161 ttgccgttca tttgtctgag gaattcgcgg ctgcaatcaa cgccgccata ggcaagacca
     5221 gtaccgcacc cgccgcatcc agtatcaacg tggtgggcgt atatgcaata ttttcaaaca
     5281 acttggattt gtcggtatgt tttttcgtaa ccgtgtaata aatatcggca ggcacacttt
     5341 gctcaaaatg ataatcggcg ttcagttttt gcggtgtggc gtagtatttg cctttggcgg
     5401 agacgcagcg cgtgtaaatg gtccgattgt cgagttcgac cgcttcaaac tcaagctgtt
     5461 tcagcttggc gatgtcggca ggtctgtcgg tatcgtagcg caggcaaagg tcgtcggtac
     5521 tgaaattctg gctggcgggc gattcgagtt tgaccggcag ggcttggtag gcaaagcgcg
     5581 ggttgggctc aaccatttga aactgcttgt ccaacccggc cttcaaaatg cccgtcagct
     5641 tcgccgaatc ttcaggattg acgacgaacc agtatttccc gcccatcatc accaggctgc
     5701 ccttttccaa ttgggcattg tctttggcaa ccacaccgaa ggcgcggatt tggtctttgt
     5761 caacgtgttt ataggcggtc gtttggctga acgggtcgtt catgccccac atcattgccg
     5821 tacagccatt cagcatcaag gttgccgcca aaacggcggc ggtcgtttta cggaacaaca
     5881 ttttatcctc caatctaaat aacggcggat gtcgtctgaa aatatgccta aatttccgcg
     5941 tccccgtttt ccatcacggt tttcaattct tgaaacagcg cgcggaagtt tttcggcggt
     6001 ttgttttgct cctgctcttt tttggtgttg cggacgagcg tcctcagctt gcccgcgtcc
     6061 gcatgcggaa aatccgacat aaactgcgtc aacgcgccgt cgtctgccaa cagccgtacg
     6121 cgcgcctgtt ccacgcgttg caaaaaggcg ttgtgcgccg catcgtcgcc gcgcagcttg
     6181 gcaagaaacg cctcgatggg cgcgggatcg gtatcgcgca tcagcctgcc gataaattgt
     6241 gcctggcgtt tgagcgcgcc gttggatgtg atttttttat aggcggttac cgcctcgtac
     6301 aaatccgcat ccaaaccgat ttttttcagc gtatcgtttg agagcttggt caacgccata
     6361 cccaaatcct gcaaatcgtt catctgcttt ttcatttggg ttttgctgat ccattcgtct
     6421 tcttgttcaa acatcttgat tctcgaattt ttccaatagc gcattttaac agaatgccgc
     6481 cgcgttcaga cggcattttg caccgtccgc cctgcaaacg gcgcgctttt gcccctgccg
     6541 cccgaaacgg ttaaaatgcc gcaaagcatc atcaagaaag atttctatgc tgttcaacca
     6601 caccgcttcc gaactcctcg acctctgccg ccgcacgctc gacttggcaa aagcgacggg
     6661 cgcaaccgcc gccgaagccg atttcagcga atcattggga caaagcgtca gcgtgcggct
     6721 gggcgaaatc gaacaaatcg agttccagca ggacaagtcg ctggacatta ccgtttacgt
     6781 cggcaaacgc aaaggccgcg ccagtactgc cgacttctcc gaaaaagccc tgcaagacac
     6841 cgtcaaagcc gccatcgaca tcgcccgcca caccgccgaa gacggttgcg ccggacttgc
     6901 cgatgcctgt ctgatggcaa aacacatcgg cgaccccgac ctttaccacg aatgggattt
     6961 ggatacggaa gccgccgtcg gcttggcaaa acaatgcgaa caagccgccc tgaacgagga
     7021 tgagcgcatc gaaaactccg aaggcgcggc ggtgcaaacc ggccattacc aatacgttta
     7081 cggcaacacc cacggttttg ccgcacaccg gcaaggcacg caccacagca tttcctgcag
     7141 cgtcgttgcc gccgacgaaa acgggatgca gcgcgactac tggtacgatt ccgcctgccg
     7201 ccatccggat atggacagcc ccgaaaccat cggtcaaacc gccgcccgcc gcactttgcg
     7261 ccgactcggc agccgcagca tcccgacagg cagctacccc gtcctcttcg ataccaccgt
     7321 ttcgggcggt cttatcggac acctcgtcgg cgcactctcc ggcggcgcgc tctaccgcca
     7381 aagcagtttc ctgatcgaca gcatcggcaa aaaagtcctg cccgatttcc tcaacctgcg
     7441 cgaagaaccg cacatccccc gctctttccg cagcagctat tttgatgcgg aaggcgttgc
     7501 caccgcaccg cgcttcgtga ttcaaaacgg cattgtcgaa ggctatttcc tcagcagtta
     7561 cagcgcgcgc aaactcggta tgcagaccac gggcaacgcc ggcggcgcgc acaacctgta
     7621 tttgaaccat acgcacgaaa cgcaatccga cctgctgaaa gaaatgggga cgggattatt
     7681 ggttaccgaa ctgatgggac agggcgcgaa caccattacc ggtgactact cgcgcggcgc
     7741 ggcgggtttt tgggtggaaa acggcgtgat tgcctacccc gtccacgaga ttaccgtagc
     7801 cggacgcttg caggatatgt accgcgacat tgtcggcgtg gcggatgacg ctttgcggcg
     7861 ttcgtccaac aaaatcggct cgattctgat tgcggggatg acggttgccg gaagctgaac
     7921 gccctgccga tgccgtctga agacgaaggc agcacgtttt gccccgtgcc tgcccgtttg
     7981 ccgttttatg ccgcaccgcc ttttgccgac aacattgcac attttttcat cagcactgat
     8041 tttcccttat ccgtcaaaat actaagccac ttgtccacaa cagggtacac acaagttcag
     8101 gataaggcgt gcggggattg gggtttttcc gccgccggca aaacggcaga caaaaacggc
     8161 gggacattgc ctgtcccgcc gttttcaaat atacatttga ccgtacatca ggaattaagc
     8221 agcctgaatg ttggcggcct gtttgccttt agggccggtg gttacgtcga aagagacgcg
     8281 ttggccttct ttcagggttt tgaaaccttc catattgatt gctgagaagt gagcgaacaa
     8341 atcttcgccg ccttcatcag gcgtgatgaa accaaaacct ttagcgtcgt taaaccattt
     8401 tacgataccg gttgccatta gaaacttcct atactcaaaa attaacaaaa tcagcaaaac
     8461 aaggcatacg gcaaaccgag ccgtctggcg ttccttccag cttgatatac tcggcggatg
     8521 cccaatcctg cttaaccgtc tttttacatt tgttaagaca aaacgtcaag ctatgttttc
     8581 aaaaaagtgg gaaaataggc aaaagtaccg aaaatacaac aaaaatgccg tctgaacgcg
     8641 gctttcaaat gggacacact atgaccgcgc aacaccaatc cgacacgctc ttgcaccgtc
     8701 tgaacaccct gccgccgaaa cgttacggcg ttttcctatt gaacgacgat tacaccacga
     8761 tggaatttgt cgttgaaatc ctgaccgaaa tcttcatgct cggacaagaa caggcggtag
     8821 cggtaatgct cttggttcat cacgaaggca aaggcctgtg cggcacttac acgcgcgata
     8881 ttgcccaaac caaacagcaa caagtcatgc agcgggcaaa agccgaaggg catccgctgc
     8941 aatgtattgt cgaggagatt taatatgctt gcacccgaat tggaacagat tttgcagcag
     9001 ctttaccgcg aggcgcgtaa ggctcattat gaatttatca gcctcgagca tctgcttttg
     9061 gtactcatcg aagaagatgc ctccgtgccg aacgtcttaa aactctgcgg cgcggatttg
     9121 aaagtggtgt ccgaacagct cgccgccagc gttgccgaaa acacccccct gattcccgaa
     9181 caccttttag acacggtcga aacccggccc acgctcggct tccaacgcgt gatgcaacgg
     9241 gcgatggtgc atacccagtc tgccggaaaa gccgcagtcg aaccgttgga cgttttggtc
     9301 gcgctgatga gcgaaaccga cagccacacc gtctatttcc tcaagctgca atcggttacg
     9361 cgttttgaag ttttgcgctg tattgcccac ggctctcccg atgaagatga agacgatggc
     9421 aactattctt cagacggcat ggacgacgat aatgaaaacc gcaccaaacc gggcaaaaac
     9481 cctttatcgg cgtacaccgt caacctcaac gccgaagtca aagccggccg tatcgaccct
     9541 ttgattggtc gcaaacacga aatggaacgg ctggtgcaaa tcctatgccg ccgccgcaaa
     9601 aacaatccgc ttttggtcgg cgaagcgggc gtgggcaaaa ccgcgctggc ggaaggtttg
     9661 gcacatcaaa tcgtcaacgg cggcattcca gacgcgctca aagatgccga agtgtacgcg
     9721 ctggatatgg gctcgctgtt ggcgggcacg aaataccgcg gcgactttga agcgcgggtc
     9781 aaatccgtct tgaaacagct cgaaaaaatc ccgcacgcca ttttgtttat cgacgaaatc
     9841 cacaccatca tcggcgcagg cagcaccagc ggcggcacca tggacgcgtc caacctgctc
     9901 aaacccgcgc tggcaaaagg ttcgctgcgc tgcatcggcg cgaccactta cgacgaatac
     9961 cgcaccattt tcgacaaaga ccacgcctta agccgccgct tccaaaaaat cgacgtggtc
    10021 gaacccaccg tttccgaaac cgtacaaatc ctgcgcggct tgaagccgat gtttgaagcc
    10081 ttccaccaag tccgctatac gcaaggcgca ctcgaagccg ccgccgaact ctccgcacgc
    10141 tacatcaacg agcgtttcct gcccgacaaa gccatcgacg tgatggacga agcaggcgcg
    10201 gcgcaacgga ttctgcccaa atccaaacag aaaaaagtca tcggcaaagc acaaattgaa
    10261 accgtcatcg ccaaagttgc gcggattccc gaaaaaaccg tgtcgcacga cgacaaacaa
    10321 gtgctgcaat tcctcggtcg cgatttgaaa aacatggttt acggtcaaga aaacgccatc
    10381 gacgcgctgg ttgccgccgt caaaatgtcg cgttccggcc ttgccctgcc cgacaaaccg
    10441 ataggcagtt tcctcttctc cggtccgacc ggcgtgggca aaaccgaagc cgccaaacag
    10501 cttgcctact cgatgggcgt accgctgcaa cgctttgata tgtccgaata catggaacgc
    10561 catgccgtat cacgactcat cggcgcgcct ccgggctacg tcggctttga acaaggcggc
    10621 cttttgaccg aagccgtcaa caaacagccg cattgcgtat tgctcttgga cgaaatcgaa
    10681 aaagcccacc ccgacatttt caacgtcctc ctgcaagtca tggacgcagg caaactgacc
    10741 gacaacaacg gcaagagtgc cgatttccgc aacgtcatcc tgattatgac caccaatgca
    10801 ggtgcggaga gtctcagccg ccccagcctc ggctttaccg ccaagcgcga acgcggcgac
    10861 gaaatgcagg caatcaacaa gctcttcacg cccgagttcc gcaaccgctt ggacgcgatt
    10921 atcccgtttg cacccttgtc cgaacccatc atcgtcaaag tcgtggacaa attcctgctc
    10981 cagctcgaac accagctcct cgacaaaaaa gtcgaagccg aattcacgcc ggcattgcac
    11041 aaatatctgg cggaaaaagg ttttgacccg caaatgggcg cgcgcccgat gcaccgcctg
    11101 attcaggaaa aaatccgcaa accgctcgcc gacgaactgc tgttcggcaa actatccgac
    11161 ggcggcttcg tgcggataga ctgggatgcg gcaaaagaag aagccgtgtt gaaatttaag
    11221 aaaagtaagg tcaa
//
LOCUS       DAXHNE010000053        10986 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.053, whole genome shotgun sequence.
ACCESSION   DAXHNE010000053 DAXHNE010000000
VERSION     DAXHNE010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10986
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.053"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(1022..1792)
                     /gene="xth"
                     /locus_tag="ACFM4Z_001064"
     CDS             complement(1022..1792)
                     /gene="xth"
                     /locus_tag="ACFM4Z_001064"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC6021114.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFKNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(1846..2133)
                     /locus_tag="ACFM4Z_001065"
     CDS             complement(1846..2133)
                     /locus_tag="ACFM4Z_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFC6021115.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSGHLNRLRAGGLVDFTRYHRIIEYRLISEDAATILRTIRDLENKRAVWC"
     gene            complement(2539..3420)
                     /gene="nadC"
                     /locus_tag="ACFM4Z_001066"
     CDS             complement(2539..3420)
                     /gene="nadC"
                     /locus_tag="ACFM4Z_001066"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFC6021116.1"
                     /translation="MPSENTLFPLPDTLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQAVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVQQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTIYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
     gene            complement(3495..4433)
                     /locus_tag="ACFM4Z_001067"
     CDS             complement(3495..4433)
                     /locus_tag="ACFM4Z_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021117.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGMLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVGRLRIWANSADTEEVRQKRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIAEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
     gene            4635..5747
                     /gene="nadA"
                     /locus_tag="ACFM4Z_001068"
     CDS             4635..5747
                     /gene="nadA"
                     /locus_tag="ACFM4Z_001068"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFC6021118.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETADRL
                     KDKIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYICRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPEAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKQFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKKKDVFNGMGPA"
     gene            5905..6234
                     /locus_tag="ACFM4Z_001069"
     CDS             5905..6234
                     /locus_tag="ACFM4Z_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFC6021119.1"
                     /translation="MHWLFLTVAILSEVCGSSMLKLSGGFSKLWPSIGVVVSFSVCFW
                     ALSMTLKTMPLATAYAIWAGVGLVLTALVSVVFFGEKADFIGIVSIGLILLGVVLLNT
                     MSHMSGH"
     gene            6283..7791
                     /gene="nadB"
                     /locus_tag="ACFM4Z_001070"
     CDS             6283..7791
                     /gene="nadB"
                     /locus_tag="ACFM4Z_001070"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFC6021120.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCDEAAVRAILSQGKPAIEWLLAQGVAFDR
                     NHNGLHLTREGGHTCRRIAHVADYTGEAVMQSLIAQIRRRPNIRVCERQMALDIQTES
                     GAACGLTVLDCRTQETHRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VGNLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQAGERFMPHYDRRAELAPR
                     DIVARAIAAEIAKQTQDFVSLDISHQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLCAQA
                     IADGQAFQAEPFQRPSESLSAEAGIFSDDLQNTFSRPVLQTFNQRHLGILRNDTGLRR
                     AIAQLQLWKQNQAEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
     gene            complement(7969..8634)
                     /gene="pgmB"
                     /locus_tag="ACFM4Z_001071"
     CDS             complement(7969..8634)
                     /gene="pgmB"
                     /locus_tag="ACFM4Z_001071"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFC6021121.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRYKNDNYVEMIQAVKPEDVYPGILPLLEA
                     LKANGKKIALASASKNGPFLLERMGLTDFFDAIADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSGIAELTYAYLQNVWAQ
                     SGR"
     gene            complement(8647..10905)
                     /locus_tag="ACFM4Z_001072"
     CDS             complement(8647..10905)
                     /locus_tag="ACFM4Z_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFC6021122.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRLFTVFGVRFDVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDGLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALGGRQTDWQVSNSFEAEVGGTPKTFEKRVIVTTSRDYQGLEAVKVAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDESYLAKEGLE
                     VLVEVSRFWADRVHFSKRRGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPGAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLNGSHTVALEK"
BASE COUNT         2410 a         2898 c         3066 g         2612 t
ORIGIN      
        1 tgaaactgat gcagatatag tggattaaca aaaatcagga caaggcgacg aagccgcaga
       61 cagtacaaat agtacggcaa cgctgtactg gtttaaattt aatccactat attttgtaat
      121 agctgccatt atgcctacta ggatatatac cttctatata atccttattt aaataaaaac
      181 ccttataaaa atctgtgtta tctgtcggga ttgaaaaaaa gattttatct ttctcatcag
      241 gtttatattt gctatcaata tcaaaaagag aataaggatt acaatgagtt cgacatacta
      301 ggtaaaaatt tatatcttgg atcatgaaat attcgatatt tatcaggatc gaaagatgct
      361 aaaaattcat caatatcggt actggatatt ttgtcatcac taataaacat cgctagagat
      421 gggcgacttt gattttccat ttgttgatta gtaaaggata taaatactaa cttgccattg
      481 tcaaaccaca tagatttata tagtggatta aatttaaacc agtacgtcgt tgcctcgcct
      541 tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac
      601 tatagttatt gcctttggga gaatcaattc tgacagcaca taacataggt tcatctttac
      661 agtcgttata ttgggtttca tcaaactcaa ctgcatagca caataaaggc aataggaaaa
      721 aaaggagatt aatttattac tatacataag gcagcctgac tattcattct aaaataaatc
      781 cggtttttaa tatttgattc tgctccggat tatatacata ataaatttta ctgctccaaa
      841 atctgatatt aaccctatgg atttcataaa tttttcagaa aaataggctg tgcttgaaca
      901 atccgccgac tccggctgtt tgtcagggaa aaggcaatta ttttcggtta gtcagcagcc
      961 tatcctattg aatcacttaa agatatggat gtttgtcaac gccatatcaa aacacggtct
     1021 tttaccaatc gaattctgcc gtcaccggcg cgtggtcgct cggacgctcc agcgcgcgcg
     1081 tctccaaatc aacgcggaca tccttcaacg ccgccgccat cgcaggcgac accaaaatat
     1141 ggtcgatacg caggcccagt ttgcgttgga acatcgcgcc gcgatagtcg aaccaggtat
     1201 agaacgcgcc ttcgggatgg acttggcgca ggctgtcggt cagtcccaaa tccagcaggt
     1261 ttttgaacca ctgccgttcg acggacgaac agtggatttt ttcgtgccat ttttcagggt
     1321 cgtaacagtc cgcatcggca ggcgcgatat tgaaatcgcc cagtagcacc agtttgccgt
     1381 agcgggtcat ttcatcgcgg acaaattcgg ttaacgcggc aaaccattgt tctttatatt
     1441 tgaatttcgg gctgtcgagg gcttcgccgt tgacgcaata gacattgatg acgcgcacac
     1501 cgccgacggt tgccgcaatc acgcgccgtt gcggatcgtc cggcagcgcg ggcagcccga
     1561 tatgcacgtc ttccggcgcg ttgcggctga cgattgccac gccgttgtag gttttctgcc
     1621 cgctccaaac acagtgccag cccatcattt gcaaagcggc ggccggaaat ttgtcctgat
     1681 cgagtttgag ttcctgcaaa accaaaatat cgggcggatt gtcggcaagc aggttttgca
     1741 cctgcggcag ccgcacattg agcgaattga cgttccaagt ggtgattttc ataacatttc
     1801 cgaaaaatgc cgtctgaaac gttcagacgg caaaaggaaa ggattttaac accataccgc
     1861 gcgtttgttt tccaaatcgc ggattgtccg cagaatcgtt gccgcgtctt cggaaatcag
     1921 gcggtattcg ataatgcggt ggtaacgcgt aaagtccacc agaccgcccg ccctcaggcg
     1981 gttcaaatgg ccggaaacgg cggtggccgg cagggataag gattttgcca gttcggcgat
     2041 attgcgttcg ctgtccaaca gttggatcag tatcgccata cgttcgggat gcgccataag
     2101 tttgagtatg gtgtggagcg gtattgtgtt catcggtatt cctaaaacct ttgccgcccg
     2161 ccaagcggat tgacgggcga cagatatgga tgattgtgat tatctttata aatcgtatta
     2221 taaatcaaat atttatggat gattgtacag gattgtatgt tccttttccc actttttttg
     2281 caaacacgcg taaaggcggg ctttcagacg gcataaaact gccgcccccg tcctttcccc
     2341 tgccctaccg atgcctccgc gctgtcgggc aaacaaaata cctgcctgaa acgctgtttt
     2401 ttctaaagca tcacgacctt cacgaaaaat gccgtctgaa gaatatttca gacggcattt
     2461 cctccttgag gcaataaccg gattacggtt aggcggttcc ttgtctgaca tatcagacaa
     2521 ctgccgacac cctaaaactc atgccaagaa atccaaacct atgtccaacg agcggctgct
     2581 gtgggtcaga tagccgaggg cgatgccgtc cacgccggtt tgcgccacgc gcttcaggcg
     2641 gtcgaagccg atgccgcccg atgcttcgca atagatggtg tgggggtggg cggtttgcgt
     2701 gtggcagcgg tttgccgctt ctttcagggt ttcgtcgtcc atgttatcca gcaaaatccg
     2761 ttccgcgccc gctgcgatgg cttcgtccag ttgtgccaac gtatccactt cgatttccac
     2821 gcaggtcaat gctccgacag cctgttttgc ctgctgcacg gcttgggcga tgctgccgca
     2881 ataggcgagg tggttgtctt tgatgagcac ggcgtcgtcc aaacccatgc ggtggttcac
     2941 accgccgcct gccctgacgg cgtatttttg caggacacgc agcaggggga tggttttgcg
     3001 gctgcacacg atgtctgtac cgtattcggc gacttcggca acggcacgcg cggtggcggt
     3061 ggcgatgccg cttaagtgcg tgaggtagtt gagcgcggtg cgttcggcgg cgagcagcgc
     3121 gcgggcgttg ccttcgacgg cggcaagcgt ctgacctgcg cggacggctt gcccgtctcg
     3181 gatttcggct tggaagcgga cggacggatc catcgtctga aaggcgagac gcgccaagtc
     3241 catgccggcg ataacgccgt cttcgcggct gacaaggaag agtttggcgg ttttgtcggg
     3301 ggcgatgacg gcggcggacg taatatcgcc gcgcctgccc aagtcttcgc tcaaggcttg
     3361 ttccaccatg gggcgcaaca gggtgtcggg cagggggaag agggtgtttt cagacggcat
     3421 ggggtttcct ttgttgaaaa agattgatgt tgcaccgatg ggcaaaactt cgtttgatgt
     3481 cgtctgaaaa cgggctaacg gctgcccagc ggcagtccga tgtccgaaat cagcctgtcg
     3541 ggcaggacgt cgtcgcggaa gcggcaaagc tgcgcgggac ggcctttgct gcccgataca
     3601 ccggtatccg acggctcgat gaggttttgc tgctgaatct ggcggcggaa gttttgcttg
     3661 tgcagcaatc tgccgctgat ggcttcgaca ctgttttgca gttgcagcag cgtgaattcg
     3721 ggcggcatca gttcaaaaat cacggggcgg tatttgattt tggcgcgcag gcgagacagg
     3781 gcggtcgcca gtacgcggcg gtggtcgtgg cgcatgggct gccccgtaag cgcgaagtcg
     3841 aagtttgcct gcggctcggc ggcctccgct atcaggccgc tttcatacag catttcatag
     3901 cgttgcaaaa cgtattcttc cgaccagttt tccggttcga ccccccaaca caaatgaatg
     3961 cgcttgagcc gcttttggcg cacttcctcc gtgtccgccg agtttgccca aatgcgcagg
     4021 cggccgacga cggcgtcgcg ctgcccgccg tcggtacgca aatcttccca cgggaaatag
     4081 ccgtagcagt cctgccattt cgcgtcggga tgcaggatgc tgtcggctgc ctcgcgcacc
     4141 agccccaaat agctgacgta caagacgggc atgccgtgtt cgttgcggcg gtgggtatcg
     4201 acaaaggtgt aaagctgttc cacatagccc ataggctgcg aagtctgctt ggcgacccac
     4261 agttttacgc ctgcctgcaa ggaattgcgc aggggggaga gcgggccgtt gggcaggagc
     4321 atgccttggg cgacggtcaa tacccgcagg ccgccgtcgg taacggcaat caatacggga
     4381 accagctcga cgatgctttg cggcggggct tcggcttcgg ggtaggcatc catgctcggc
     4441 tttctgcggg tgggaaaggc tttattgtaa accaaagcgg tttgtcggaa cgaagcgggt
     4501 cggttttacg gtattgcaaa cctgttttca gacgaccttg catattatac tcaagttgag
     4561 cataatgtaa aaacagtttg aaaaacagac ggcaaacctt catgccgtct gaaaaacagc
     4621 tacaaggaaa catcatgcaa accgccgccc gccgctcgtt cgactacgat atgccactca
     4681 tccaaacgcc gacttccgcc tgccaaatcc gtcaggcgtg ggcgaaggtt gccgacacgc
     4741 ccgaccgcga gacggcagat cgtctgaaag acaaaatcaa ggctttgctg aaggagaaaa
     4801 acgcggtctt ggtggcgcat tattacgttg atccgctgat tcaggatttg gctttggaga
     4861 cgggcggatg cgtgggcgat tcgctggaaa tggcgcgctt cggtgcggaa cacgaagccg
     4921 gtacgctggt ggtggctggt gtgcgcttca tgggcgagag cgcgaaaatc ctctgccctg
     4981 aaaaaacggt gctgatgcct gatttggagg cggaatgttc tttggatttg ggttgcccgg
     5041 aagaagcgtt ttcagcgttt tgcgaccaac accccgaccg cacggtggtg gtgtacgcca
     5101 acacttccgc cgccgtgaaa gcgcgtgccg attgggtggt aacgtcttcg gtggcgttag
     5161 aaatcgtatc gtatttgaaa tcacgcggcg agaagctgat ttggggaccc gaccgccacc
     5221 tcggcgacta catctgccgc gaaacgggcg cggatatgct gttgtggcag ggttcgtgca
     5281 tcgttcacaa cgaattcaaa gggcaagagc tggcggcgtt gaaggcggaa caccccgaag
     5341 cggtggtgct ggttcatcct gaatcgccgc aaagcgtcat cgaactgggc gacgtggtcg
     5401 gctcgaccag caaactgctc aaagccgccg tatcgcgtcc tgaaaaaaaa ttcatcgtgg
     5461 cgaccgattt gggcatcctg cacgaaatgc aaaagcaggc gcccgacaaa caatttatcg
     5521 ccgcgccgac ggcgggcaac ggtggaagct gtaaaagctg cgcgttctgc ccgtggatgg
     5581 cgatgaattc gctgggcggc atcaaatacg ccctgacaag cggacgtaac gaaatcctgt
     5641 tggacaggaa gctgggcgaa gccgccaaac tgcctttgca gcgtatgctc gactttgcgg
     5701 cagggctgaa gaaaaaagat gtgttcaacg gcatgggccc cgcctgattt gtcgttcgta
     5761 atgggtgcag cctgaaaata ccgcccgcct gctttcccaa gacgggattt gcacacaaaa
     5821 aatctttaac aacaagactg tttttttatt ttattgcatt gatataatta tactttcttt
     5881 catttatcag gtaaaaacat gcaaatgcac tggctctttc tgactgtagc aattttaagc
     5941 gaagtctgcg gttcttccat gctcaaactg agtggcgggt ttagcaaact gtggccttct
     6001 attggcgtgg tagtcagctt ttcggtgtgt ttttgggcct tgtctatgac actgaaaacc
     6061 atgccgctgg ctacagcata cgccatttgg gcaggcgtgg gactggtttt aacggcttta
     6121 gtcagcgtgg tgtttttcgg tgagaaagct gatttcattg ggattgtcag catcggtttg
     6181 attttgctgg gcgtggtgct tctcaatacc atgtcgcata tgtcggggca ttgattaatc
     6241 cactataccg tttcagacgg cctctcaaac aaggacaaca ccatgcaaac cgattgcgac
     6301 gtattgattg ccggaaacgg gctggcggca ctgacgctcg ccctgtcgct gcctgaatcg
     6361 ttccgcatcg ttattttgtg caaaaaccgg ctggacgaca ccgccagccg tcatgcgcaa
     6421 ggcgggattg cggcggcgtg gtcgggagag gacgacatcg aaaaacacgt tgccgatact
     6481 ttggaagcgg gcgcgggttt gtgtgatgaa gccgccgtcc gcgccatcct gtcgcagggc
     6541 aaaccggcaa tcgaatggct gctggcgcag ggcgtggcgt tcgaccggaa tcataacggc
     6601 ctgcacctga cgcgtgaagg cgggcatacc tgccgacgaa tcgcccacgt cgccgactac
     6661 acgggcgaag ccgtcatgca gagcctgatt gcccaaatac gccgccgccc gaacatccgc
     6721 gtttgcgagc ggcagatggc gttggacatt caaaccgaat caggcgcggc gtgcggactg
     6781 accgtcctcg actgccgaac acaagaaacc caccgcatcc gcgcccgcca taccgtactc
     6841 gcaggcggcg gcttgggaca gatttacgcc gccaccacca cgccgcccga atgcacgggc
     6901 gacgccatcg ccatggcgat acgcgcaggc tgcgcagttg gaaacctcga atttatccaa
     6961 ttccacccca caggcttggc aagaccgtct gaaaacggac gcaccttcct catttccgaa
     7021 gccgtgcgcg gcgaaggcgg catcctgacc aaccaagcgg gcgaacgatt tatgccgcat
     7081 tacgaccgcc gcgccgaact cgcaccgcgc gacatcgttg cccgcgccat cgcagccgaa
     7141 atcgccaaac aaacgcaaga cttcgtctcg ctcgacatca gccatcaacc cgcagcgttc
     7201 gtccgccggc atttcccatc catccatcga cactgtttat cccaatgcgg cctagacatc
     7261 acgcgccaag ccatccccgt ccgccccgtg caacactaca cctgcggcgg catccaaacc
     7321 gacccctgcg gcagaacctc cctgccgcag ctctacgcct tgggcgaaac cgcctgcact
     7381 gggctgcacg gagccaaccg ccttgccagc aactccctgc tcgaatgcgt cgtgaccgca
     7441 aggctctgcg cccaagccat cgcagacgga caagcattcc aagccgaacc attccaaagg
     7501 ccgtctgaaa gcctctccgc cgaagcaggc atcttttcag acgaccttca aaacacattc
     7561 agccgccccg tcctgcaaac gttcaaccaa cgccatctag gcatcctacg caacgatacc
     7621 ggcctgcgcc gcgccatcgc ccaactgcag ctttggaagc aaaaccaagc cgaaccgcac
     7681 accgcgtccg aatacgaaaa ccgcaacctg ctcgaatgca gcctcgccgt cgcccaagcc
     7741 gcatacaggc ggagacagaa catcggcgcg cattttaata gtgattgtta aggttggctg
     7801 gttagaaaaa aaatcgttca aataaatttg aatagcatgg ttaagagatt ggcatttagt
     7861 ttttagggtt gtgtaagttg ggttatcatt ctgttctaaa agcagaaagg ccgtctgaaa
     7921 cactgttcag acggcctttt acttccttga cactttatct gacgcgtttt acctgcccga
     7981 ctgtgcccac acgttttgca ggtaggcgta ggtcagctcg gcgatgccgg acaccagcgc
     8041 gatgtcgctg cccaagtcct cggctttgcc cacgccgatg ggcaaggcgc cggcggcttt
     8101 gatggcggct acacctgcgg cggcgtcttc aatgccgatg cagcggcgga tgtctgcgcc
     8161 cacgccctcg gcggcggcga ggaagatgtc gggggcgggt ttggaatgcg cgacggcggc
     8221 agggtcggca atggcgtcga agaagtcggt cagccccatg cgttcgagca ggaaggggcc
     8281 gtttttactg gcggacgcga gggcgatttt tttgccgttt gctttcaatg cttccagcag
     8341 cggcaaaatg ccggggtaca cgtcttcggg tttgactgcc tgaatcatct cgacgtagtt
     8401 gtcgtttttg tagcgggtca gttcggcaaa ctcggcttcg ccgacggttt tgccgccgtg
     8461 cgcgaggatg cgtttgagcg aatcgtcgcg cgacacgcct ttgagctgct cgttaaactt
     8521 gcggtctatg ccgatgccca gttcttcggc gagctttttc catgcgcggt aatggtattc
     8581 ggcggtatcg gtgatgacac cgtcgagatc gaagagcaca gcggtaaacg tcattttgcg
     8641 ccctccttat ttttccaacg caacggtgtg gctgccgttg agcgtgatgt ctttgccgta
     8701 cacctgcaaa tcaagcggtt cgcctttgag cagggtgaag acgacgtttt ctttgccgac
     8761 ggcgacttta atcagacggc ctcggtagtt gatatggaag gcgtagcctg tccatgcgcc
     8821 cggcaggaac ggtgcgaagc tgagtttgcc gccccaagtt ttcatttggg cgaaaccttg
     8881 gacgatggcg agccacgagc cggtcataga agtaatgtgc aggccgtctt cggtgtcgtt
     8941 gttgtagttg tccaagtcca ggcgggctgt gcgccggtac atttccacgg ctttttcttc
     9001 tttgcccagt tcggcggcga gaatagagtg gatacacggc gacaacgagc tttcgtgcac
     9061 ggtcatcggt tcgtagaagt cgaaattgcg gcgtttttcg tcgatattga aacggtcgcc
     9121 gaagaaatag atgccttgca atacgtccgc ctgtttgata aagggcgaac gcaggatttt
     9181 gtcccacgac catttctggt tgagcggcaa atcgtcgggc gaaagcgcgg acacggggcg
     9241 gatgtctttg tcgaggaagc cgtcgtgttg cacgaatacg ccgagttctt cgtcatgcgg
     9301 acggtacata ttcgcgctga tgtccgccca tttttccaac tcggcggcac gcacgttcaa
     9361 atccggacgc gggtatttcg ccaaggcttc gcgggtgtag tccaataccc aagcggcaag
     9421 ggtgttggtg taccagttgt tgttgatgtt gttttcgtat tcgttcgggc cggttacgcc
     9481 gtgaatcatg tatttacccc tgcgtttgga gaagtggacg cggtccgccc agaagcggga
     9541 cacttcgacc aagacttcca agccttcttt ggcaagatag ctttcgtcgc cggtgtagtt
     9601 ggtgtagttg tagatggcgt aaggaatcgc gccgttgcgg tggatttcct cgaaggtgat
     9661 ttcccattcg ttgtggcact cgatgcccgt aaacgttacc atcggataga gtgcgcccgc
     9721 caagccctgt tcgcgcgcgt tgtgctgcgc ctgcggcagt tggttgcggc ggtattgcag
     9781 caggttgcgg gtaacttcgg gttccgccag cgcgaggtag agcggcactg cgtaggcttc
     9841 ggtgtcccaa taggtcgcgc cgccgtattt ttcgccggta aagcctttcg ggccgatgtt
     9901 caggcgcgcg tcttcgccgt agtaggtgga gaacagttgg aacagattga aacggatgcc
     9961 ttgttgcgct tcgtcgctgc cttcgatgac cacgtcggcg atttcccaac ggtgcagcca
    10021 gcctgctttg tgcgcgtcca gcaaggtttc aaacgcaacg cctgcgactt tttccgacaa
    10081 ggcacgaccg gcgactttca ctgcttccaa gccctgataa tcgcggctgg tggtaacaat
    10141 cacgcgtttt tcaaaggttt tgggcgtgcc gccgacttcg gcctcgaaag aattggagac
    10201 ctgccagtcg gtttgtctgc cgccgagggc tttgaagcta ccggcgaaag tttgctcggc
    10261 gttgacgata aattgttcca caccaaaagg gttggcaacg gtttgggcgg caatgtagga
    10321 gaggccgtct gaaacgcctt tgtccaatac ctgccagaat ttttcttcgt agttggagtc
    10381 ttcgtttttc acgtcggcat cgatgatgga atcgatgcgg acttggtggg ttttgccgtc
    10441 aacggatacg gcttcccagc ggataaccgc cagctctttt tgcgcgacgg acaggaattt
    10501 gcacacatcg aaacgcacgc cgaatacggt gaacaagcgg cgcaacacgc cgtgctgcat
    10561 atcgagttcg acggagaagc cggcaacatc gtttttcgcc aagtccacct cttgaccatc
    10621 gacaaagatt ttgactttgc tgaaattgag cgcgttgatg gctttgccga aatatttggg
    10681 atagccgttt ttccaccagc cgacgcgggt tttgtcgggg aaccacacgc cggcgatgta
    10741 ggtgcccaag tggctgtcgg cggagtaggt ttcctcaaag ctgccgcgca tacccatata
    10801 gccgttgccc aaactggtca ggctctcttg cagccgtttg tgttcttttt ccagttttgc
    10861 cgaacgcagc gtccaagggc tgatttccat aattcttgtg tacatttatg aagctcctgt
    10921 ttggattgat ttgagggaat ggtgaaatct tatagtggat taacaaaaat caggacaagg
    10981 cgacga
//
LOCUS       DAXHNE010000054        10790 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.054, whole genome shotgun sequence.
ACCESSION   DAXHNE010000054 DAXHNE010000000
VERSION     DAXHNE010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10790
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.054"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(105..1322)
                     /locus_tag="ACFM4Z_001073"
     CDS             complement(105..1322)
                     /locus_tag="ACFM4Z_001073"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFC6021123.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLATGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLTAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1522..2250)
                     /gene="rph"
                     /locus_tag="ACFM4Z_001074"
     CDS             complement(1522..2250)
                     /gene="rph"
                     /locus_tag="ACFM4Z_001074"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFC6021124.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2332..2796)
                     /locus_tag="ACFM4Z_001075"
     CDS             complement(2332..2796)
                     /locus_tag="ACFM4Z_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFC6021125.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTAQELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3013..3789
                     /locus_tag="ACFM4Z_001076"
     CDS             3013..3789
                     /locus_tag="ACFM4Z_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC6021126.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERITQINPQCEVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVELKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            3927..4454
                     /locus_tag="ACFM4Z_001077"
     CDS             3927..4454
                     /locus_tag="ACFM4Z_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021127.1"
                     /translation="MEFVNNLVIFSFLLLMLIPIFFVVYGIYHKIRYRKICILRTSFI
                     LLVVILCSMYYIYCRYLDQQKVAYYCIDEQCISIVHLYKDYGINSPTYARIYAGKILF
                     RFQVRAKNYAELLMEDDISISKKILGNKFIIYGSLPVIYGNVDNIEVKEATGYIDRSS
                     TDYIVSRNLKFRHLY"
     gene            4808..5662
                     /locus_tag="ACFM4Z_001078"
     CDS             4808..5662
                     /locus_tag="ACFM4Z_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HFC6021128.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPKQGAAYVVVNFIALLELAKEGLVRIVQEDGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            5703..6911
                     /gene="pncB"
                     /locus_tag="ACFM4Z_001079"
     CDS             5703..6911
                     /gene="pncB"
                     /locus_tag="ACFM4Z_001079"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFC6021129.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIREDLEAELDALCQLRFTHDELDYLRSLRFIKSDFVDYLELFQLQRRFVEIGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLCDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            7003..8721
                     /gene="argS"
                     /locus_tag="ACFM4Z_001080"
     CDS             7003..8721
                     /gene="argS"
                     /locus_tag="ACFM4Z_001080"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFC6021130.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIAGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGIAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLAFMGNTVVRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERAAALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSNYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDATAPTVLSEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAESASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            complement(8609..9082)
                     /locus_tag="ACFM4Z_001081"
     CDS             complement(8609..9082)
                     /locus_tag="ACFM4Z_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021131.1"
                     /translation="MAERCRPGAKVQHSAAGGAHACRGKENREKRRAETLPEEKADLR
                     GGKRGMRRAESACVGRKGVIIFCLLACLLACLLACLLAWRLFVSHFGRGGGYGVSRRF
                     FQIRIRMARLLSAIGRCGFTLRPTHRCPANPSLVSACRRSASPVAGGCCGLRFRL"
     gene            9275..10693
                     /locus_tag="ACFM4Z_001082"
     CDS             9275..10693
                     /locus_tag="ACFM4Z_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC6021132.1"
                     /translation="MNIHTLLSKQWTLPPFLPKRLLLSLLILLAPNAMFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLNFKIK"
BASE COUNT         2582 a         3051 c         2667 g         2490 t
ORIGIN      
        1 tttttgttcc aaagtcaaaa tatgccgtcc gaacattcgg gcggcagaca aaacggcact
       61 gcccgataaa ggcagtgccg ttgtccgttt caaaccgtga aacatcagcc caaattaaag
      121 gctttatgca ataccctggt tgccagttcc atgtattttt catcaatcaa tacggaaact
      181 ttgatttcgg aggtggaaat catttggatg ttgataccct cttcggcgag cgtgcggaag
      241 attttggcgg ctacaccgac gtgcgaacgc atacccaaac cgactgcgga gactttgcat
      301 acggtgtcgt cgccatcaat agaagccgcg ccgatactgt cttggcgttc cgacaggatt
      361 tccaaagtct gcttgtaatc gccgcgcggt acggtaaagg aaaaatcggt tgtgccttcg
      421 ctgccgacat tttggataat catatcgact tcgatgttgg catcggcaac cgcgcctaaa
      481 atctgatagg cgacgccagg tttgtcgggt acgccgcgca cgttgatgcg ggcttggttt
      541 ttatcgaatg cgataccggt tacggcagct ctttccatgt tgtcgtcctc ttcaaaggta
      601 attaaggtgc cattgccacc gtcttgcagg ctgctcagta cgcgcaggcg cactttgtat
      661 tttccggcga attctactga acggatttgc aaaactttcg aaccgaggct tgccagttcg
      721 atcatttctt caaatgtaac cgtatccatg cggcgcgctt cgggtacgac gcgggggtcg
      781 gttgtgtaaa cgccgtctac gtcggtatag atttggcact cgtcggcttt gagcgcggcg
      841 gcaagcgcga cggcggaagt gtcggaaccg ccgcgtccga gcgtggaaat atcgccttca
      901 ctgctgatgc cttggaagcc ggcaacgatg acgactttgc cggcggtaag gtcggcacgc
      961 attttttcgt catcaatgct ttcgatgcgg gctttggtgt gggcggtatc ggttttgagg
     1021 gcgacctgcc agcctgtgta gcttttggca tccacgccga tgtctttcaa tgccatcgcc
     1081 aaaaggccga tggttacttg ttcgccggta gctaagacga cgtccagctc gcgcggatcg
     1141 ggatgctctt gcatttcgtg cgccagtgcg accagtcggt tggtttcgcc gctcatggcg
     1201 gatacgacga ctacgatgtc gtgtccttcg gcgcgggctt tggcgacacg tttggctacg
     1261 tttttgatgc gttcgggcga gcctactgat gtgccgccgt atttatgtac gattaacgcc
     1321 atgtttcgtg ctttcttgtg ggggttgtcg ggcagcttgg tttgctggaa aaagggttat
     1381 tattactatt ttttacatgg aattcaagaa cggactgcgc tttcccgcct gccgtttgac
     1441 agcggtcagc gaaaaacctg ttctttcaga ttgttgacaa aatgccgtct gaacggtttt
     1501 cagacggcat ccggacgaca atcaggcggc ggacaacgca ttttgctggt gttgcagcag
     1561 ttcgcctatg cctttttgcg ccagtgcaac cagtttgccc aattcgtcca aactgaacgg
     1621 cgcgtcttcc gccgtcccct gtatttcgat gatttttccc gatgcggtca tgacgatatt
     1681 cacatcactg tcgcaaccgg agtcttcggg ataatccaaa tccaaaagcg gcacgccgtt
     1741 cactacgcct actgacacag cggcaacggc ttcgcggatg gggttttcac tcaaaatgcc
     1801 gtctgaaacc agtttgccga cggcgatttg cagcgcgaca aacgcaccgg taatcgaagc
     1861 cgtgcgcgta ccgccgtctg cctgaatcac atcgcagtca atcaagattt gtcgttcacc
     1921 gagtttttcc atatccacga ccgcgcgcag ggaacgcccg atcaaacgtt ggatttcttg
     1981 tgtgcgcccg gactgtttgc ccgccgaagc ttcgcggcgc atccgggaag cagttgaggc
     2041 aggcagcatc ccgtattccg ccgttaccca gccttggttt ttaccgcgca gaaacggcgg
     2101 gacgttttca tctatggaag cggtacaaat cactttggta ttgccgcatt caataaggca
     2161 cgaaccgtcc gtatgcggca ggaaatgagg ggtgattttg atatcgcgca ggctgtcggc
     2221 ggcgcgcgag atgcggatgt aatcaggcat actgccctcc cgttaaaaac agataaatta
     2281 aaaagcctta aatatgaaaa atcacattta aggccttcaa actgaaaatt tctacgcctc
     2341 ttcggctttg ctgcggataa tcaaaagcgg caggtggctt tggcgcatta ccgtttcggc
     2401 aaaactgccc attaaaaggt gcatcagccc ggtacgtccg tgcgtaccca acaccagcag
     2461 gtcggcaccg ttttcatcgg cataatcaac caaatcctgc gccatttcac gcgcaccctt
     2521 attggcaacc agcaggtgtt tgacggtatt ttccacaccc agttcctggg cggtgcgctc
     2581 ggcggcatcc aaaacttcgt tgccttgcgc gacggcggcg gcttcgtagc tttcgtgttg
     2641 caaaaattcg ggggcgagtg ccatatattc ggcaggattg gcaacgtgca ccaaagtcag
     2701 gcgcgcaccg ttgaccccgg caagctcggc ggcatgtttc agggcattga tggacgtttc
     2761 actgccgtca acggcaacaa ccaaatgttt gtacatatcg tattctcctt ttgcaccgcc
     2821 tcgcggtgcc ctcttgtcgg atgggcgcag ggacagtttg cgctgtttca ttatagaccc
     2881 gccgtcgggc tttatacaac agccgaacag cccgaccgct ttccagtata atatgccgct
     2941 tccgtgcagt caggcatttt ttgccggctt tcgttcactt tttgatttga cgcaatcttg
     3001 caggattcga ccatgtccga caacgctttg acctcttcgc gacgcttcgg cggcatcgcc
     3061 agactctacg gagactctgc cttggcgcac ttttcacagg cacacgtctg cgtagtcggc
     3121 gtgggcggtg tcggctcgtg ggcggtcgag gctttggcgc ggacgggcat cggacgtttg
     3181 actttgattg atttggacaa cgttgccgaa tcgaatgtca accgccagct gcacgccctg
     3241 accggcgact tcggcaaagc aaaagttacc gccttgcgcg aacgcattac acaaattaat
     3301 ccgcaatgcg aagtgtttga aattgaagat ttcgttaccg aagacaattt gccggaatac
     3361 ttcggaaaag gttttgattt cgtcatcgac gcgatcgacc aagtgcgcgt caaagcagca
     3421 atggcggctt attttgtgga actcaaacaa ccgtttgtcc tcagcggcgg cgcgggcgga
     3481 caaaaaaatc cggcgttaat ccaaaccgcc gatttgagcc gcgtaaccca cgacccgctg
     3541 cttgccaacc tgcgctacac cttgcggaaa cgctacggat tcagccgcga tacgaaagca
     3601 aatatgcgcg tgccttgcgt gtattcgacc gaaaatatcg tgccgccgca gtctagggag
     3661 gcttgttcgg cagatgccgc tccgcaaggc ttgtcgtgcg ccggctacgg tgcaagcatg
     3721 ctcgttaccg cttcgttcgg gctatattgc gcacaggcgg cggtggaaca catcgcagac
     3781 aaaaaataag caatgccgtc tgaaacagga ttcagacggc atttgaacaa actatggtta
     3841 tgatttaaga caacaaagga tacggataaa aaataacata aaatatatga ttcctaataa
     3901 tataccaagt atcggagagc tatttaatgg aattcgttaa taatttagtt attttttcat
     3961 ttttattact aatgcttatt ccgatatttt ttgtagtata tggtatatac cataagatac
     4021 gttatcgcaa aatatgtatc ctaagaacaa gttttatatt attagtggta atactttgca
     4081 gtatgtatta catatattgc cgttatcttg accaacaaaa agtagcttat tattgcatag
     4141 atgaacaatg tatttctatt gttcatctat acaaagatta tggtataaac tctcccacat
     4201 atgcgagaat ttacgcagga aaaatattgt ttagatttca agtaagagct aaaaattacg
     4261 ctgaattact tatggaagat gatatatcaa ttagtaaaaa aattttgggg aataaattta
     4321 tcatttatgg gtcgctacct gtaatatacg gtaatgtaga taatattgaa gtaaaagaag
     4381 ctactggtta tatagataga tccagtactg attatattgt ctcaagaaac ttaaaattca
     4441 gacatttata ttaattaaga ggttttagca agagtgccgt caaaatatag ggcgcatcat
     4501 cgaattcgcg aaagacaaac gctacgatga acgtttcaag gatttgaaaa aagaatccat
     4561 aggctatctg aaccggcatc ccggtttggt gtccgactac ctgaaggcgg caatcaagct
     4621 gtcggttcag aaaaaccaac atcagcacgc ctaaaaccgt attcacaacc tgctcctttt
     4681 caaaacattt gcatttaaaa gccgttataa tgccgtctga acatctgccc gaccacatta
     4741 tacgtgaatg tcggcagatt gttttctttt gtaaacttat attaaaatcc acttaccgat
     4801 tcacgccatg ccgcccatcc ctgccccatc tgcaccatcc gagcacactg tcgcatgggt
     4861 attcggccaa cccgttaccg atttgcccca ggatttgttt attccgcccg atgcattgaa
     4921 agtcgtattg ggcagcttcc aaggcccttt ggatctactg ctgtatctga tccgcaaaca
     4981 gaatatcgac gtactggata ttccgatggt gaagattacc gagcagtatc tgcactacat
     5041 cgcccaaata gaaacctatc agtttgattt ggcggcggaa tatcttttga tggcagcaat
     5101 gctgattgaa atcaaatcgc gcctgctgct gccgcgtacc gaaaccgtcg aagacgaaga
     5161 agccgacccg cgtgccgagt tggtgcgccg cctgctggct tacgaacaga tgaagctggc
     5221 ggcgcagggt ttggacgcgc tgccccgagc cggacgggat ttcgcgtggg cttacctgcc
     5281 gctggaaatt gccgtcgaag ccaagctgcc cgaagtctat attaccgact tgacgcaagc
     5341 gtggctgggt attttgtctc gggcaaaaca cacgcgcagc cacgaagtaa tcaaagaaac
     5401 catctccgtg cgcgcgcaaa tgacggcaat cctgcgccgt ttgaacggac acggaatatg
     5461 caggtttcac gacctgttca atcccaaaca gggcgcggct tacgtggtcg tcaacttcat
     5521 cgcactgttg gagcttgcca aagaaggatt ggtcagaatc gtgcaggaag acggtttcgg
     5581 agaaatccga atcagcctca atcatgaggg ggcgcattca gacggcattt ccggcacacg
     5641 aggcgggcgc gatgtgttct aatacgcccc aagccgccac caaaaatcgg gagacacgcc
     5701 atatgaccgg catcatacat tcgctgcttg acaccgacct ctacaaattc actatgctgc
     5761 aagtggttct gcaccagttt ccgcagacgc acagccttta cgaattccgc tgccgcaacg
     5821 cctcgaccgt ctatccgctt gccgacatca gggaagactt ggaagccgaa ctcgacgcgc
     5881 tctgccaact acgcttcacc cacgacgaac tcgactacct ccgtagcctg cgtttcatta
     5941 aaagcgactt tgtcgattat ctcgaactct tccagctcca acgccgcttt gtcgaaatcg
     6001 gcacagacga taaagaccgt ctgaacatcc gcatcgaagg tccgatgata caggcgatgt
     6061 tttttgaaat cttcatcctc gccattgtca acgaacttta cttccgccgc ctggaaaccc
     6121 ctgcagtcat agaagaaggc gaacgccggc ttcaagccaa agccgcgcgc ctcaaagaaa
     6181 tcgccgccgc acaaaacccc gacgaaccgc ccttcctgat ttccgacttc ggcacgcgcc
     6241 gccgctacaa gctcgcgtgg caggaacacg tcatccgcac cctgctcgaa gccgccccaa
     6301 gcatcgtgcg cggcaccagc aatgtctttc tcgccaaaaa actcggcatc acccccatcg
     6361 gcaccatggc gcacgagttc ctccaagcct tccaggccct cgacgtacgc ctgcggaatt
     6421 tccaaaaggc cgcgctcgaa agctgggtgc acgaataccg gggcgatttg ggcgttgccc
     6481 tgaccgacgt ggtcggtatg gatgccttcc tgtgcgattt cgacctctat ttcgccaaac
     6541 ttttcgacgg gctgcgccac gacagcggcg acccttacgt ttggggcgac aaagcctacg
     6601 cccactatca aaagctcaaa atcgacagcc gcaccaaaat gctgaccttc tccgacgggc
     6661 tggacatcga acgctcttgg gcattacacc aatatttcaa agaccgcttc aaaaccggct
     6721 tcggcatcgg caccaacctg accaacgata tggggcatac gcctttgaat atcgtcttga
     6781 aactggtcga atgcaacggg cagtccgtcg ccaagctgtc cgactctccg ggcaaaacca
     6841 tgaccaacaa cagcaccttc ctcgcctacc tgcgccaagt gttcgacgta cccgaacccg
     6901 aaacgccgta aaccggcaga aaaagcgcac aattcctgtt tctgccgcat aaaatctttt
     6961 aaaataccgc ctgatttgaa tttaaccgaa agaccgaact tcatgaacct acatcaaacc
     7021 gtcgaacacg aagccgccgc cgcctttgcc gccgcaggca tcgccggcag ccccgttgtt
     7081 ttgcagccga ccaaaaatgc cgaacacggc gatttccaaa tcaacggcgt gatgggtgcg
     7141 gcgaaaaaag ccaaacaaaa cccgcgcgaa ctggcgcaaa aggtcgccga cgcattggca
     7201 ggcaacgccg tcatcgaaag cgcggaagtc gccggtcccg gttttatcaa cctgcgcctg
     7261 cgccccgaat ttctcgcgca aaacattcag acggccttga acgacgcgcg tttcggcata
     7321 gcgaaaaccg acaaaccgca aaccgtcgtc atcgactatt cctcgcccaa tctggcgaag
     7381 gaaatgcatg tcggtcacct gcgttccagc atcatcggcg acagcatttc gcgcgtgttg
     7441 gcatttatgg gcaacaccgt cgtccgtcaa aaccacgtcg gcgactgggg tacgcagttc
     7501 ggcatgttgg tcgcttattt ggtcgagcag caaaaagaca atgccgcgtt tgagctggcg
     7561 gatttggagc agttttaccg cgccgccaaa gtgcgctttg acgaagatcc tgccttcgcc
     7621 gacaccgcgc gcgaatacgt tgtgaagctg caaggcggcg atgaaaccgt tttggcattg
     7681 tggaaacagt ttgtcgatat ttcgctctcg cacgcccaag ccgtttacga cacgctgggc
     7741 ttgaagctgc gccccgaaga cgtggcgggc gaatcgaagt acaacgacga tttgcagcca
     7801 gtggtcgatg atttggttca aaaaggtctg gcggtcgaag acgacggcgc gaaagtcgta
     7861 ttcttggacg agttcaaaaa caaagaaggc gaacccgccg cgtttatcgt gcaaaaacaa
     7921 ggcggcggct tcctttatgc ttccaccgac ttggcgtgtt tgcgctaccg tgtcggcacg
     7981 cttcatgccg accgcctgct gtacgtcgtc gaccaccgcc aagccctgca ctttgagcaa
     8041 ctctttacca cttcccgcaa agcaggctat ctgccggaaa acgtcggcgc ggcatttgtc
     8101 ggcttcggca cgatgatggg caaagacggc aaaccgttca aaacacgcag cggcgacact
     8161 gtaaaactgg tcgatctgct gaccgaagcc gtcgaacgcg ccgccgcttt ggtgaaagag
     8221 aaaaatcccg aattgggcgc ggacgaagcc gccaaaatcg gcaaaaccgt cggcatcggc
     8281 gcggtcaaat acgccgactt gagcaaaaac cgcaccagca attatgtgtt cgactgggac
     8341 gctatgcttt cgtttgaagg caacaccgcc ccctacctgc aatatgccta cacccgcgtg
     8401 caaagcgtgt tccgcaaagc cggtgaatgg gacgcaaccg caccgactgt tttgagcgaa
     8461 ccgctggaaa aacagcttgc cgccgagctg ctgaaattcg aagacgtgct gcaaagcgtg
     8521 gcggacacgg cgtatccgca ctacctcgcc gcctacctct atcaaattgc gaccctgttc
     8581 agccgcttct acgaagcctg tccgatactc aaagccgaaa gcgcaagccg caacagccgc
     8641 ctgcaactgg cgaagctgac cggcgacacg ctgaaacaag gcttggattt gctgggcatc
     8701 gatgtgttgg acgtaatgta aaaccgcacc gcccgattgc ggacaacagc ctcgccatcc
     8761 ttatccgaat ctgaaaaaag cggcgcgata caccgtatcc gccgcccctc ccaaaatgcg
     8821 aaacaaacaa acgccaagca agcaagcaag caagcaagca agcaagcaag caagcaagca
     8881 agcaaaaaat tataaccccc ttcctgccga cgcacgcact ttccgcgcgg cgcattcccc
     8941 ttttcccgcc cctcaaatcc gccttttctt caggcagggt ttcagcccgc ctcttttccc
     9001 tgttttcctt tccccgacac gcgtgcgcgc cccctgccgc actgtgctgc actttcgcgc
     9061 ccggacggca tcgttccgcc atccggttct ctgttttaaa tacccctgtt tcagaaagaa
     9121 atgcagatgt ttcaacacac aggacgacac ataaagcacc gccctatgtg ttgccctgat
     9181 ttggaagggg ttacgcctcc caaataaagt ctgatcctgc cgccccgaag gacagatgtc
     9241 cgagtggcga agtttcaacc gaaaaggaaa tacgatgaat attcacaccc tgctctccaa
     9301 acaatggacg ctgccgccat tcctgccgaa acggctgctg ctgtccctgc tgatactgct
     9361 ggcccccaat gcaatgtttt gggttttggc actgctgacc gccaccgccc gcccgattgt
     9421 caatttggac tatcttcccg ccgcgctgct gatcgccctg ccttggcgtt tcgtcaaaat
     9481 tgccggcgta ttggcgtttt ggctggcggt tttgtttgac gggctgatga tggtgatcca
     9541 gctcttccct tttatggacc tcatcggcgc catcaacctc gtccccttca tcctgaccgc
     9601 ccccgcccct tatcagataa tgaccgggct gttgctgctg tatatgctgg cgatgccgtt
     9661 tgtgttgcag aaagccgccg ccaaaaccga cttccggcac attgccgtct gcgccgccgt
     9721 tgtggcggca gccggctatt tcaccggcca tttgagttac tacgaccggg gtcggatggc
     9781 caatatcttc ggcgcaaaca acttctacta cgccaaaagt caggcgatgc tctacaccgt
     9841 cagccagaat gccgacttta ttaccgccgg cctggtcgat cccgtcttcc tccccttggg
     9901 caatcaacag cgtgccgcca cgcatctgaa cgagccgaaa tctcaaaaaa tcctctttat
     9961 cgtcgccgaa tcttgggggc tgccggccaa tcccgaactt caaaacgcca cttttgccaa
    10021 actgctggcg caaaaagacc gtttttcggt ttgggaaagc ggcagttttc ccttcatcgg
    10081 cgcgacggtc gaaggcgaaa tgcgcgaact gtgtgcctac ggcggtttgc gcgggttcgc
    10141 actgcgccgc gcgcccgacg aaaaatttgc ccgctgcctc cccaaccgtt tgaaacaaga
    10201 aggttacgcc acctttgcga tgcacggcgc gggcagttcg ctttacgacc gcttcagctg
    10261 gtatccgagg gcgggctttc aagaaatcaa aaccgccgaa aacctgatcg gtaaaaaaac
    10321 ctgcgccatt ttcggcggcg tgtgcgacag cgagctgttc ggcgaagtgt cggcattttt
    10381 caaaaaacac gacaagggac tgttttactg gatgacgctg accagccacg ccgactatcc
    10441 cgaatccgac attttcaacc acaggctcaa atgcaccgaa tatggcctgc ccgccgaaac
    10501 cgacctctgc cgcaatttca gcctgcacac ccaattcttc gaccaactgg cggatttgat
    10561 ccaacgcccc gaaatgaaag gcacggaagt catcatcgtc ggcgaccatc cgccgcccgt
    10621 cggcaacctc aatgaaacct tccgctacct caaacagggg cacgtcgcct ggctgaactt
    10681 caaaatcaaa taacaacaat gccgtctgaa cgcaccaaca gccttcagac ggcattttgc
    10741 agacagaccg acccttcaag cccacttttt tcatcatctc cgataaattg
//
LOCUS       DAXHNE010000055        10758 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.055, whole genome shotgun sequence.
ACCESSION   DAXHNE010000055 DAXHNE010000000
VERSION     DAXHNE010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10758
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.055"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(32..235)
                     /locus_tag="ACFM4Z_001083"
     CDS             complement(32..235)
                     /locus_tag="ACFM4Z_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HFC6021133.1"
                     /translation="MDMTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWSDDAV"
     gene            complement(213..446)
                     /locus_tag="ACFM4Z_001084"
     CDS             complement(213..446)
                     /locus_tag="ACFM4Z_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC6021134.1"
                     /translation="MEATVYLEDNEYIALCDLLKLAGLAESGGQAKALIAEGLVSRNG
                     ETETRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            640..768
                     /locus_tag="ACFM4Z_001085"
     CDS             640..768
                     /locus_tag="ACFM4Z_001085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021135.1"
                     /translation="MTLMHILKHELPDTLVIGISHQNEIKNLFERKINLTACHPSE"
     gene            986..4420
                     /gene="dnaE"
                     /locus_tag="ACFM4Z_001086"
     CDS             986..4420
                     /gene="dnaE"
                     /locus_tag="ACFM4Z_001086"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFC6021136.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLVAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRGAGIKPIGAADVWIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     AAYVGKDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSSDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPGQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSRDDYLIKLSNKGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVRDKYGAQAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLEKAMEAEPQIQELIEVEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIIDVGKIPLDDQAAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESITAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDLPRWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLDR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            4463..4717
                     /locus_tag="ACFM4Z_001087"
                     /pseudo
     CDS             4463..4717
                     /locus_tag="ACFM4Z_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4854..5786)
                     /locus_tag="ACFM4Z_001088"
     CDS             complement(4854..5786)
                     /locus_tag="ACFM4Z_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC6021137.1"
                     /translation="MLSQLEYGIAKENRPNRQADRFAGSVLPLAASLTHKPNIMKPIP
                     TDTFQPAILPQAFETEIKSTCTGRIYRIQTATLGEIPSEGYPVLFVLDGEAFFPALFN
                     IMQSLMNNPVTRSNAPCLIVGIGYTTGSVRDLAQRAADYTPPLGDNATADERQQFGQA
                     DRFAAFIDSELTAFLESRYTLNRNETAVFGHSFGALFGLYSLLSHRRFRRHWLVSPSI
                     WWHNRRILDFMPSENRLDGIDVCLNIGALERGSGCKRREERDMAGLSERMAAELDRHG
                     AAVFFREYPNADHGNVSFYSLTDCVEYLRKAWQR"
     gene            complement(5856..7970)
                     /locus_tag="ACFM4Z_001089"
     CDS             complement(5856..7970)
                     /locus_tag="ACFM4Z_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFC6021138.1"
                     /translation="MSVFRINMTAATVLAALSSSVFAAQTEGLETVHIKGQRSYNAIA
                     TEKNGDYSSFAATVGTKIPASLREIPQSVSIITNQQVKDRNVDTFDQLARKTPGLRVL
                     SNDDGRSSVYARGYEYSEYNIDGLPAQMQSINGTLPNLFAFDRVEVMRGPSGLFDSSG
                     EMGGIVNLVRKRPTKAFQGHAAAGFGTHKQYKAEADVSGSLNSDGSVRGRVMAQTVGA
                     SPRPAEKNNRRETFYAAADWDINPDTVLGAGYLYQQRRLAPYNGLPADANNKLPSLPQ
                     HVFVGADWNKFKMHSHDVFADLKHYFGNGGYGKVGMRYSDRKADSNYTFAGSKLNNTG
                     QADVAGLGTDIKQKAFAVDASYSRPFALGNTANEFVIGADYNRLRSTNEQGRSTLSKS
                     VALDGFRALPYNGILQNARAGNKGFNHSVTEENLDETGLYAKTVFRPLEGLSLIAGGR
                     VGHHKIESGDGKTLHKASKTKFTSYAGAVYDIDGSNSLYASASQLYTPQTSIGTDGKL
                     LKPREGNQFEIGYKGSYMDDRLNTRVSFYRMKDKNAAAPLDSNNKKTRYAALGKRVME
                     GVETEISGAMTPKWQIHAGYSYLHSQIKTASNSRDEGIFLLMPKHSANLWTTYQVTSG
                     LTIGGGVNAMSGITSSAGIHAGGYATFDAMAAYRFTPKLKLQINADNIFNRHYYARVG
                     SESTFNIPGSERSLTANLRYSF"
     gene            8130..8789
                     /locus_tag="ACFM4Z_001090"
     CDS             8130..8789
                     /locus_tag="ACFM4Z_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6021139.1"
                     /translation="MTTPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     SLIGYSLPEIIRTLLEMPSETAVADITRTYSAHYLNPNNRNMSLFPDALPCLDKLKAQ
                     GYWLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGICGELGLDPK
                     EALVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLSAPHLAVLDGLSELPGFLAQHY
                     A"
     gene            complement(8877..9845)
                     /gene="ispH"
                     /locus_tag="ACFM4Z_001091"
                     /gene_synonym="lytB"
     CDS             complement(8877..9845)
                     /gene="ispH"
                     /locus_tag="ACFM4Z_001091"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFC6021140.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVRQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(9874..10401)
                     /gene="lspA"
                     /locus_tag="ACFM4Z_001092"
     CDS             complement(9874..10401)
                     /gene="lspA"
                     /locus_tag="ACFM4Z_001092"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFC6021141.1"
                     /translation="MVLFYRLLKNMSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQ
                     YRERVNVIPSFFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEF
                     ATLGKTGAAMIIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLA
                     VLDNIVHRKTQEEKY"
BASE COUNT         2445 a         3068 c         2836 g         2409 t
ORIGIN      
        1 tgttaattca ctatatcaag ccgaaccgtt ttcagacggc atcgtccgac caacccgctt
       61 ctttcaattt ccgccgttgc gcgtcgtagc gggccttatc cgcccaataa atcgtctgaa
      121 tacacgcctc gccgcagtcg ctgccgcagc attcccacga ctcgggtcgg acgggttcgt
      181 ctaaaagcgg ctcgcccaaa agggcttcgg ctttatgctt cagggtcata tccatcggcg
      241 atttccaagc gcgcaccgtc aaactcgatg acttcgccgc cgcgtatttt ggcggtttta
      301 cgggtttcgg tttcgccgtt gcgcgacacc aacccttcgg caatcaacgc cttcgcctgt
      361 ccgccgcttt cggcaagtcc ggccaatttc aagaggtcgc acagggcgat gtattcgttg
      421 tcttcgagat agacggtggc ttccataatg ttcccttgca gaaagaggcc gttattgtag
      481 cacctgccgc cgccgtaccc aaaattaccg caaaacaggc agcacatccc gccccgcctg
      541 ttccgtaaaa atgccgtctg aaaccccata tgccgtcatc cgatcaaaga aatcctgccc
      601 aacagcaggc tgcaaatcct gttccccgac gaatccgcat tgacgctgat gcacatcctc
      661 aaacacgaac tgcccgatac actggtcatc ggcatcagcc atcaaaacga aatcaaaaac
      721 ctgtttgaac gtaaaattaa tttaactgct tgccatccgt ccgaataagg tatatagtta
      781 tttataacta gtttgatagt tctttatatc tatcaatact ccttgggaag cctccaccac
      841 acggtaggag gcattttttt gccgtcgtaa aagctcaaaa acatttgcag gtcatgccgt
      901 ctgaatctga aacggcatga cctacaccgc catctaaaga caaccctgct acaatacgcc
      961 ttttattgtt cacgccgatt ttgccatgac cgagccgacc tacatcccct tgcgcctgca
     1021 taccgagttc tcaattaccg acggtatggt gcggattaaa aaactggttg ccaaagcgca
     1081 ggaatacggt ttgcctgctt tgggcatcag cgatttgatg aacgaattcg gcttggtgaa
     1141 attctataaa gcctgccgcg gcgcagggat taaacccatc ggcgcggcgg acgtgtggat
     1201 aggcaatccg aatgcgcccg acaagccgtt tcgcgccatg ctgattatcc gcaacgatgc
     1261 gggctatctg cgcttgagcg agcttctgac ggcggcttat gtcggcaaag accgcaatat
     1321 ccatcatgcg gaactcaatc ccgaatggct ggaaaacggc gacaacagcg gcttgatttg
     1381 tttgagcggc gcacattacg gcgaagtggg cgtgaatctg ttgaacggca atgaagacgc
     1441 ggcgcgtgcg gcggcgttga aatatgcggc atggtttccc gatgcgtttt atttagagct
     1501 gcaacgcctg cccgaacgcc ccgaatggga ggcttgcgtt tccggcagcg tgaagctggc
     1561 ggaggaactg ggtttgccgg tggtggcgac ccacccgacg cagtttatga gcagcgatga
     1621 tttcaacgcg cacgaggcac gggtgtgtat tgcgggcggc tgggtgctga cggacaaaaa
     1681 acgtccgcgc gatttcacac cgggccagtt tttcattccg ccggaaacga tgctggagcg
     1741 tttcgccgat ttgcccgagg ccttggaaaa cacggtagaa attgccaaac gctgcaatct
     1801 gcacatcaca ttgggcaaaa acttcctgcc ccttttcccc acgcccgacg gtttatcgcg
     1861 cgatgactat ctcatcaaac tctcaaacaa gggcttgcag gaacgcatgg ttcagcttta
     1921 tcccgacgag gcggagcgtg cggcaaaaat gccggaatat caggaacgtt tggattttga
     1981 gctgaacatc atcatccaga tgaaattccc cggctatttc cttatcgtac aagactttat
     2041 caactgggcg aaaacgcacg gctgtccggt cgggccggga cgcggttcgg gcgcgggttc
     2101 gctggtggcg tattcgctga aaattaccga cctcgacccg ctgaaatacg cgctgctgtt
     2161 tgaacgtttc ttaaaccccg aacgcgtctc catgcccgac ttcgacgttg acttctgtca
     2221 ggcaaaccgc ggccgcgtga tcgaatatgt gcgcgataaa tacggcgctc aggcggtcag
     2281 ccagattgtt accttcggca cgatgtcgtc caaagcggtc atccgcgacg tggggcgcgt
     2341 gttggagctg ccgtttatgc tgtgcgacaa actgtccaag ctgattccgt tggaagccaa
     2401 caaacctctg agtttggaaa aagccatgga ggccgagccg cagattcagg aattgatcga
     2461 ggtggaagaa gcggacgaac tgattacact ggcgaaaaag ctggaagatt tgacgcgcgg
     2521 tctgggtatg cacgcgggcg gcgtgttgat tgcgccgggc aagatttccg attacagccc
     2581 cgtgtatcag gcggatgaat ccgcctcgcc cgtatccatg tacgacaagg gtgacgtgga
     2641 agacgtgggc ttggtgaaat tcgacttttt gggtctgcgc aacctgacca ttatcgaaat
     2701 ggcgcagaac aacatcaaaa acaccaccgg cgacatcatc gatgtcggca aaattcctct
     2761 cgacgaccaa gccgcctacc aaatcttccg agatgcgaac accaccgccg tgttccagtt
     2821 cgagtcgacc ggcatgaaaa aaatgctgaa aacggcgcac acgaccaagt ttgaagaact
     2881 gattgccttc gtatcgctct accgccccgg cccgatggac aacatccccg acttcgtcgc
     2941 gcgtatgaag gggcaagaat tccaatatat ccatccgctg ctcgagggca tcctcgcgcc
     3001 gacctacggc attatggtgt atcaggaaca agtgatgcag gcggcacaga ttatcggcgg
     3061 ctactcgctc ggcggcgcgg acttgctgcg ccgcgcgatg ggtaagaaaa agcccgaaga
     3121 aatggtgaaa caccgcgaaa tcttcgccga aggcgcggca aaacaaggca tttcgcgcga
     3181 aaaatccgac gaaatcttca actatatgga aaaattcgcc ggctacggtt tcaacaaatc
     3241 ccacgccgcc gcctacgccc tgatttccta ccagaccgca tggctcaagg cgcactaccc
     3301 cgccgaattt atggcggcga ccatgtcgtc cgaattggac aacaccgacc agctcaagca
     3361 tttctacgac gactgccgcg ccaacggcat cgagttcctg ccgcccgaca tcaacgaatc
     3421 cgactaccgc tttacgccgt atccggacat gaaaatccgc tacgcgctcg gcgcgattaa
     3481 aggcacgggc gaagccgccg tcgaatccat caccgccgcg cggcaaagcg gcggcaagtt
     3541 taccggcctg ttggacttct gcgagcgcgt cggcaaagaa cacatgaacc gccgcaccct
     3601 cgaagccctg atacgcggcg gcgcgttcga cagtatcgaa cccaaccgcg ccatgctctt
     3661 ggcgaacatc gacctcgcta tggacaacgc cgaccaaaaa gccgccaacg ccaatcaggg
     3721 cgggctgttc gacatgatgg aagacgccat cgaaccggtg cggctcatcg acctgccgag
     3781 gtggagcgaa tcggaaaaac tcgccgaaga aaaaaccgtc atcggctttt acctgtccgg
     3841 ccacccgttc ggcccgtatg cccaagaagt ccgccaaatc gcaccgacca aattagaccg
     3901 tctgaagccg caagacagcg tgcgcctcgc cggattcgtt accgccgtac gcaccatgat
     3961 gggcaagcgc ggcaaaatcg ccttcgtcag ccttgaagat ttgagcggac aggttgaaat
     4021 catggtcggc ggtcagacgt tggaaaactg cgccgactgc ctcaaagccg accaagtgct
     4081 gattatcgaa tccaaagtca gccgcgacga ctacggcggc ggcgacgggc tgcgtattct
     4141 ggcaaaccaa gtcatgaccc tgcaaacggc acgcgaacgc tacgcccgca gcctcagcct
     4201 cgccctcgcc ccgcatcacg acatcggcgg actggtacgg ctgctcgccg cccaccaact
     4261 gcccgacacg ccgcgcatcc cgctgcaact gtcgtatgcc aacgaaaaag cgtcgggcag
     4321 gcttcaagtg ccgccgaaat ggacggttac accgagctcc gcattgttcg gcgaactgga
     4381 aacattgctc ggcagccggt cggtgcgcgt caactggtaa cccaaaatat aaatgccgtc
     4441 tgaagcccaa aaaccggttt cattcgtact ttattcgaat gattgaataa aagtaactgc
     4501 caagaaaaac gtattttttg gttatttcgc cagtctaaat agagcaaccg ggacgattga
     4561 tatccgtgtg catgacacag acagcaccaa agggaaaaac ggcattttcc aaagtatcgg
     4621 tatcaaaacc gccctttcac tccaaaaata ccaaatcgac aaaccgggca aagaaatcag
     4681 accgtgccgc ctgaaaaaac gcccacccgt ccgttaaatt aacctatccc tgtttcagac
     4741 ggcctgaagc agggattttt atatcaaaat aaaatgagaa agggagcaat aacccttagg
     4801 tagctcttgt tattttccga tgcaaaacaa agcagtcata tatttaattc cccctacctc
     4861 tgccaagcct tcctcaaata ttcgacgcaa tcggtcagcg agtagaacga gacattgccg
     4921 tggtcggcat tcggatattc ccggaaaaag acggcggccc cgtgcctgtc caactctgcc
     4981 gccatgcgtt cggacagtcc tgccatatcg cgttcttccc tgcgtttgca accgctgccc
     5041 cgttctagcg cgccgatgtt gaggcagaca tcgatgccgt ccagccggtt ttcagacggc
     5101 ataaaatcga gtatccgcct gttgtgccac caaatcgagg gggatacgag ccaatgccgt
     5161 ctgaaacggc ggtgggaaag cagggaatac agtccgaaca gtgcgccgaa cgagtgtccg
     5221 aatacggcgg tttcattgcg gttgagggtg tagcggcttt ctaaaaaggc ggtcagctcg
     5281 ctgtcgataa aggcggcgaa gcggtctgcc tgtccgaact gctgccgttc gtctgctgtg
     5341 gcgttgtctc caagcggcgg cgtgtagtcg gcggcacgtt gtgccaaatc gcgcacactg
     5401 cctgtcgtgt agccgatacc gacaatcagg cagggggcgt tgcttcgggt aacggggttg
     5461 ttcatcagcg actgcatgat gttgaaaagt gcggggaaaa aggcttcgcc gtcgaggaca
     5521 aagaggacgg gatagccttc agacggtatt tcgccgagtg ttgccgtctg aatccgatag
     5581 attcgccccg tgcaggtgga tttgatttcg gtttcaaagg cttggggcag tatggcaggt
     5641 tggaatgtgt cggtcggtat gggtttcatg atgttcggct tgtgggtcag actggcggca
     5701 agcggtaaaa ccgaaccggc aaagcggtct gcctgccggt tcggtctatt ttccttcgca
     5761 atgccatact ccagttgtga gagcataggg tatgccgcgc agcttgttgt agtttgatga
     5821 tgctgcggct gccgtttcag acggcatatt ggtctttaaa aactgtaacg caggtttgcc
     5881 gtcaggctgc gctccgaacc gggaatgtta aaggtgctct cgctgccgac gcgggcgtag
     5941 taatggcggt tgaagatgtt gtcggcgttg atttgcagct tcagtttggg cgtgaagcgg
     6001 tatgccgcca tcgcatcgaa cgtggcataa ccgcctgcat gtatccctgc agatgaagta
     6061 atgccgctca tcgcgttcac gccgccgccg atggtcagcc cggacgtaac ttggtaagtc
     6121 gtccacaggt ttgcgctgtg tttgggcatc agcaggaaga tgccttcgtc gcgcgaattg
     6181 gaggcggttt tgatttggct gtgcaggtag ctgtaacctg catggatttg ccatttcggt
     6241 gtcatcgcgc cgctgatttc ggtctcaaca ccttccatca cgcgtttgcc caatgcggcg
     6301 taacgggttt ttttgttgtt tgagtccagc ggtgcggcgg cgtttttatc cttcatgcgg
     6361 tagaacgaaa cccgggtatt gaggcggtcg tccatgtagc tgcctttgta gccgatttca
     6421 aactggttgc cttcgcgcgg tttgagcagc ttgccgtcgg tgccgatgct ggtttgcggt
     6481 gtgtagagtt gggaggcgga agcgtacagg ctgttgctgc cgtctatatc gtaaaccgcg
     6541 ccggcgtagc ttgtaaattt ggttttcgaa gctttatgca gggttttgcc gtcgcccgac
     6601 tcgattttgt gatgtcctac acgtccgcct gcaatcaacg acaaaccttc cagaggacgg
     6661 aacaccgtct tggcatacaa accggtttcg tcgaggtttt cttcggtaac ggagtgattg
     6721 aaacctttgt ttccggcgcg ggcgttctga agtatgccgt tataaggcaa agcgcggaaa
     6781 ccatctaaag cgacgctttt tgacaaagtc gaacgccctt gttcattagt actgcgcaag
     6841 cggttgtagt ctgcaccaat cacaaattcg ttggcggtgt tgcccaaggc aaacggacgg
     6901 ctgtaacttg cgtcaaccgc aaaggctttt tgtttaatgt ccgtacccaa acccgctacg
     6961 tcggcttgtc cggtattgtt gagtttgctg cccgcaaacg tataattgga atcggctttc
     7021 cgatcggaat agcgcatacc gactttgccg tagccgccgt tgccgaagta atgtttcaaa
     7081 tcggcgaaca cgtcgtggct gtgcatttta aatttgttcc aatccgcgcc gacaaatacg
     7141 tgttgcggca gggacggtaa tttgttattg gcatcggcag gcaggccgtt gtacggcgcg
     7201 aggcggcgtt gctggtaaag atagcccgcg cccaaaaccg tatcggggtt gatgtcccaa
     7261 tccgccgccg cgtagaaggt ttcgcgccgg ttgtttttct cggcgggacg cggagacgcg
     7321 ccgacggtct gcgccatcac gcggccgcgc acgctgccgt ctgaattgag gctgcccgat
     7381 acgtccgcct cggctttata ttgtttgtgc gtaccgaacc ctgccgccgc atgaccttgg
     7441 aacgctttgg tcgggcgttt gcgcaccaga ttcacgatgc cgcccatctc gccgctgctg
     7501 tcgaacagtc cgctcggccc gcgcatcact tccacgcggt cgaaggcgaa caggttgggc
     7561 agcgtgccgt tgatactctg catctgcgcg ggcaggccgt cgatgttgta ttcgctgtat
     7621 tcgtaaccgc gcgcgtaaac cgaagagcgt ccgtcgtcgt tgctcaacac gcgcaggccg
     7681 ggcgttttgc gtgccaactg gtcaaacgta tcaacattgc ggtctttgac ctgctggttg
     7741 gtaatgatgc tgacggattg cggaatttcg cgcaaagaag cggggatttt tgtaccgacg
     7801 gtggcggcaa acgagctgta atcgccgttt ttctcggtgg caatcgcgtt gtaagaacgc
     7861 tgacccttaa tatggacggt ttccaaacct tccgtttgtg cggcaaaaac cgaagacgag
     7921 agtgctgcca aaaccgtggc ggcggtcata ttgatgcgga aaactgacat aaactgtccc
     7981 attcacataa atgataatgg ttctatttta ataaagcgca acgcggcttg ttcggaaaaa
     8041 catatcgcgc agccgacaaa ttttgtcgaa aatgcgacac gtctgcgttt tccgcataaa
     8101 atttgctttt ttactgcaac caacctgcta tgaccacgcc caaactcatc atcttcgact
     8161 gggacggcac gcttgccgat acgacccaac ccatcatcga caccatgcgc cgcagcttcg
     8221 ccgaatgcgg ttttccgccg cccgaagcgg aacgcgtccg cagcctgatt ggctacagcc
     8281 tgcccgaaat catccgcacc ctgctcgaaa tgccgtctga aaccgccgtt gccgacatca
     8341 cacgcactta ttccgcacat tacctcaatc ccaacaaccg caatatgtcc ttatttcccg
     8401 atgccctgcc ctgtctggac aagctcaaag cacaaggata ctggcttgcc gtcgccacgg
     8461 gcaaagggcg ggcgggtttg gacaacgcca tcagtcaaac cgccaccggc ggctattggc
     8521 tcgccaccgc ctgcgcgggg gaatatccct ccaaaccctc gcccgaaatg gtattcggaa
     8581 tctgcggcga actgggactc gacccgaaag aggcattggt cgtcggcgat acggcgcacg
     8641 acctgcatat ggcggcaaac gcaggcgcgg cggcagtcgg cgtggccacc ggcgcacatt
     8701 cgcgcgaaca gctccttagc gcaccgcatc tcgccgtatt ggacggtttg tccgaactgc
     8761 ccggttttct tgcacaacat tacgcctgat tggtttccgc atccggcaca cggcaaaaat
     8821 gccgtctgaa gcctgttcag acggcatttg tgttgcccaa acattcaacg cctgcgtcaa
     8881 cgtttgcaca aatcgggttt ggtttcgccc tcgcggcgca actctttggg caggacgaac
     8941 acaatgcttt cttccgcacc ctcgccttcg cgtacggttt cgtgccccca tccgcgtatg
     9001 gttgccagta cttcccgcac caacacttcg ggcgcggacg cgcctgccgt tacgccgact
     9061 ttgtttttgc cctcaaacca tgcgcgttgc aggtagcctg cattatccac catatacgca
     9121 tcgattccgc gcgatgccgc cacttcgcgc aagcggttgc tgttggacga attgggcgaa
     9181 ccgaccacaa tcacgatgtc gcactgttct gccaactctt tgacggcggt ttgccggttg
     9241 gtcgtcgcat agcagatatc ttccttgtgc ggattgcgga tattggggaa acgcgcgttc
     9301 agcgcggcga tgatgtcttt ggtttcatcg accgagagcg tggtttggct gacataggcg
     9361 agtttgtcgg ggtttctgac ttcgagtttt gccacatctc cgaccgtttc gaccaaaagc
     9421 attttgcccg gcgcaagctg ccccatcgtt ccttcgacct cgacgtgccc cttatgcccg
     9481 atcatgatga tttcacagtc ttgggcatcc agtcgggcga cttccttatg cactttcgtc
     9541 accagcgggc aagtcgcatc aaacacgcgg aaaccgcgct ccgccgcttc ttgccgcacc
     9601 gccttcgata cgccgtgtgc cgaataaacc agtgtcgcgc ccggcggcac ttccgccaag
     9661 tcttcaataa acaccgcacc tttttcacgc aggttgtcca cgacgaattt gttgtgaacg
     9721 acttcgtggc gcacataaat cggcgcgccg aactcttcca aagcacgttc gacaatactg
     9781 attgcccgat ccacaccagc gcagaagccg cgcggattgg caaggatgat ggttttctcg
     9841 ttcataagcc cggtatttcg ttttcagacg gcatcaatat ttttcttctt gggttttacg
     9901 gtggacgatg ttgtccaaca ctgccaacac cgcgccgacg cagataaagc tgtcggcaat
     9961 attaaaggcg ggataaaacc aattttgcca ataaaacaat aagaaatcga cgacatgacc
    10021 gtgtatcagg cggtcgatga cattgcctaa cgcaccgccg ataatcatcg ccgcacccgt
    10081 tttgccgagg gttgcaaact catcgcgcaa gatggcgcgt accaaatacg cgctcaccgc
    10141 caccgccagc accaaaaaaa agtatttttg ccagccgccc tgatcggcaa ggaagctgaa
    10201 cgccgcaccc gggttgtaca ccaaggtcaa atcgaaaaag gaaggaatga cattgacgcg
    10261 ttcccgatac tgaaacgacg acagcaccgc ccacttcgac cactggtcca gcacgatggc
    10321 ggcaagtgcc aatacccaat agcgcgtttt acttgaaaca gatgaagaca tatttttcaa
    10381 cagccggtaa aagagtacca ttttacccga aaaccccctt tcctgtaccc gaaacggcaa
    10441 atgccgtaat cttaaaaccc gtcattcccg acaacaccgt aatctcgaaa cccgtcattc
    10501 ccgcgtaggc gggaatccag acctgtccgc acagaaactt atcggataaa aacagttgcc
    10561 caaaccccgc gttctatagt ggattaaatt caaaccagta cggcattgcc tcgccttgcc
    10621 gtactatttg tactgtctgc ggcttcgttg ccttgtcctg atttaaattt aatccactat
    10681 agattcccac ttccgtggga atgacggttc agttgcattc cgacaacacc gtaatcttga
    10741 aatccgtcat tcccgcgc
//
LOCUS       DAXHNE010000056        10689 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.056, whole genome shotgun sequence.
ACCESSION   DAXHNE010000056 DAXHNE010000000
VERSION     DAXHNE010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10689
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.056"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(1..457)
                     /locus_tag="ACFM4Z_001093"
                     /pseudo
     CDS             complement(1..457)
                     /locus_tag="ACFM4Z_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
     gene            complement(515..1891)
                     /gene="argH"
                     /locus_tag="ACFM4Z_001094"
     CDS             complement(515..1891)
                     /gene="argH"
                     /locus_tag="ACFM4Z_001094"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFC6021142.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRCRVNRMPLGAAALAGTTYPIQREITAELLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADQAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMLA"
     gene            complement(1910..2779)
                     /gene="galU"
                     /locus_tag="ACFM4Z_001095"
     CDS             complement(1910..2779)
                     /gene="galU"
                     /locus_tag="ACFM4Z_001095"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFC6021143.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     NLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(2807..3406)
                     /gene="rdgB"
                     /locus_tag="ACFM4Z_001096"
     CDS             complement(2807..3406)
                     /gene="rdgB"
                     /locus_tag="ACFM4Z_001096"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HFC6021144.1"
                     /translation="MSEKPEKIVLASGNAGKLEEFGNLFKPYSITVLPQSAFGIPECP
                     EPYPTFVENALAKARHAAKYSGLPALADDSGICAAALNGAPGIHSARYAGDNPKSDTA
                     NNLKLAAELVGKADKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNDTPLGQNGFGYD
                     PYFYLPEHGKTAAELDTEVKNRESHRAQALAELLRKLAL"
     gene            complement(3399..3632)
                     /locus_tag="ACFM4Z_001097"
     CDS             complement(3399..3632)
                     /locus_tag="ACFM4Z_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO_0222 family membrane protein"
                     /protein_id="HFC6021145.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGALALYLRHKSLHAAQSATKENRYV"
     gene            complement(3740..4273)
                     /locus_tag="ACFM4Z_001098"
     CDS             complement(3740..4273)
                     /locus_tag="ACFM4Z_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFC6021146.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDVE
                     EAKKVIKESIERWNKQA"
     gene            complement(4375..4833)
                     /gene="nudB"
                     /locus_tag="ACFM4Z_001099"
     CDS             complement(4375..4833)
                     /gene="nudB"
                     /locus_tag="ACFM4Z_001099"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFC6021147.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            5044..5120
                     /locus_tag="ACFM4Z_001100"
     tRNA            5044..5120
                     /locus_tag="ACFM4Z_001100"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5078..5080,aa:Pro,seq:ggg)
     gene            5279..7171
                     /gene="mafB"
                     /locus_tag="ACFM4Z_001101"
     CDS             5279..7171
                     /gene="mafB"
                     /locus_tag="ACFM4Z_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HFC6021148.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGAMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPIKGIGAVRGKYGLGGI
                     TAHPIKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAHTQREWENKTGLDFNHF
                     IGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVEIKKPDGSWEMKKGRGG
                     KPMTNHSMFPKDWDEARIRAEVTSAWEKRKELEGQKWRGTSKSGVEIEGFTEPNRTAY
                     PLYNSK"
     gene            7198..7617
                     /gene="mafI"
                     /locus_tag="ACFM4Z_001102"
     CDS             7198..7617
                     /gene="mafI"
                     /locus_tag="ACFM4Z_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MafI"
                     /protein_id="HFC6021149.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            7707..7778
                     /locus_tag="ACFM4Z_001103"
                     /pseudo
     CDS             7707..7778
                     /locus_tag="ACFM4Z_001103"
                     /inference="COORDINATES: protein motif:HMM:NF026862.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 17 domain-containing
                     protein"
     gene            7775..8017
                     /gene="imm31"
                     /locus_tag="ACFM4Z_001104"
     CDS             7775..8017
                     /gene="imm31"
                     /locus_tag="ACFM4Z_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm31 family immunity protein"
                     /protein_id="HFC6021150.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            8095..8340
                     /locus_tag="ACFM4Z_001105"
     CDS             8095..8340
                     /locus_tag="ACFM4Z_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC6021151.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLVPEWEHSKTGHIGGTAMDKGK"
     gene            8343..8729
                     /locus_tag="ACFM4Z_001106"
     CDS             8343..8729
                     /locus_tag="ACFM4Z_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021152.1"
                     /translation="MWKIYKENSTDLNFAIGSIYCQAINLTEFKMWVEKIIREVDLDE
                     IPNYFFDLIDFQSLYDLIDIIGFVPENNLSKNQDNALTGIAFLRGIDVYDPPVSKEKA
                     LKALKKHPEIYQRFQHFFPFVELPPL"
     gene            8831..9220
                     /locus_tag="ACFM4Z_001107"
     CDS             8831..9220
                     /locus_tag="ACFM4Z_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021153.1"
                     /translation="MWKIIKEDSDDLEFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDGGIADIDNIVGFVSSYSLSKSKKNALTGIAFLRGIYVYDPPISKEK
                     ALKALKKHPEIYQKFQHFFPFVELPPL"
     gene            9399..9785
                     /locus_tag="ACFM4Z_001108"
     CDS             9399..9785
                     /locus_tag="ACFM4Z_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021154.1"
                     /translation="MWKIYKENSTDLNFSLGSIYCQAINITEFKMWVEKIIREMDLDE
                     IPNYFFDLTDLQSLFHLIDIIGFVPENNLSKNQDNALTGIAFLRGIDVYDSPISKEKA
                     LKALKKHPEIYQRFQHFFPFVELPPL"
     gene            9907..>10689
                     /gene="mafA"
                     /locus_tag="ACFM4Z_001109"
     CDS             9907..>10689
                     /gene="mafA"
                     /locus_tag="ACFM4Z_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC6021155.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATRYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTRNSGRKGERSAGLSVNGMGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWMGPYSV"
BASE COUNT         2718 a         2475 c         2783 g         2713 t
ORIGIN      
        1 cgtctgaaca gggttgctgg aatagtattg ccatcccagc agatacagtt tgtcggggtc
       61 ttgccaatat tgttcatcca gactgttcag cagtgaggcg gttttgttgt cgagatgtta
      121 tatcccacat ttctttcagg tttttacctt ccgattggag gcggcggatt tctttgtcca
      181 atgcgtctgc cgcccgatgg atcagcattg cgctgtgtac cgcgccgatt tttttcagaa
      241 cggaacaagt cttttcggcg gcggggaaac cccagttgca gacaaattgc agtatgctgc
      301 cgttaccgat atcggcttct gtccgccaaa tcaaaaccag ctcctgttcc cgcccgtcca
      361 tgctttcgag tttgccgtca tgctgttcaa aaagtttgtc caccgcctgc cacatcatct
      421 gttcgaagcg gtcggcttgg gtatccgtat cggtcatcgt gttcttgcct tttaaaaatg
      481 ccgtctgaac atttcttcag acggcatttg ggggttaagc caacatttcc cgccagcgtt
      541 tcacttggaa gcggacttgt tccggcgcgg taccgcccaa gtggttgcgg gcgtttaagc
      601 tgccttcggg tgtcagcacg ccgtaaacgt cgtcggcaat caaatcgctg aaaccttgta
      661 agacttcgag cggcagttcg ctcaaatcga cgcccgcttg gtcggcgtgg cgcacggctt
      721 gggcgacgac ttcgtgggca tcgcggaaag gcatgccttt tttgaccaga taatccgcca
      781 agtcggtggc ggtagcgaag ccctgcatca cggcggcgcg catattgtcg ggtttgacgg
      841 ttacgccgcg catcatatcg gcgtaaatcc gcaacgtgtc gataagcgtg tcggcggtgt
      901 cgaacaaggg ttctttgtct tcctgattgt ctttgttgta cgccaagggt tgggatttca
      961 tcagggtaat cagaccgata aggtgtccga tgacgcggcc ggatttgccg cgcacgagtt
     1021 cgggcacgtc ggggtttttc ttctgcggca tgatggacga acctgtgcag aaacggtcgg
     1081 cgatgtcgat aaagccgaaa cgcgggctca tccacaaaat caattcttca gacaggcggc
     1141 tcaggtgaac cataaccagc gaggcggcgg ctgtgaactc aatggcgaaa tcgcggtcgg
     1201 atacggcatc gagcgagttc tggcagattt gttcaaagcc caatagctcg gcggtgattt
     1261 cgcgctgaat cgggtaggtc gtcccggcaa gggcggctgc gccgagcggc atacggttga
     1321 cgcggcagcg gcagtccgcc atccgttcgt tatcgcgtcc gagcatttcg acgtaggcga
     1381 gcatatggtg tccgaagctg acgggctggg cgacttgcag gtgggtaaag cctggcatga
     1441 cggtttcggc gttttgttcc gccaaatcca gcaatgccgt ctgaaggctt tgaatcaggc
     1501 tttgtataac ggtaatctgg tcgcgcagcc acaggcggat gtcggtggcg acttggtcgt
     1561 tgcggctgcg gccggtgtgc aggcgtttgc ccgcgtcgcc gattttgtcg gtcaggcggc
     1621 gttcgatgtt catatggaca tcttccaaat cggacgacca ttcgattttg ccgctgcgga
     1681 tttcttcgag gatttccgcc ataccccggc ggatgtccgc caaatcgcct tcgtccaaca
     1741 cgccggtttc tttcagcatt tgcgcgtgtg ccagcgagcc ttggatgtcc cattcggcaa
     1801 gccgtcggtc gaaaccgatg gaggcggtgt attgtttgac gagttcggaa acgggttcgt
     1861 tgaaacgtcc ggaccaggtt ttgtcgtgca taaggattcc ttgatggggt tattcggtgc
     1921 ggtatttttc caaaagccgg cggaagggtt cgccggtttc gggatgtttc agaccgtagg
     1981 cgacggtggc ttcgaggtag cccagtttgc tgccgcagtc gtagcgcgta ccttcaaagg
     2041 ggtgcgccag gacaaattcg tgatcgagca gcttggcgat gccgtctgta agctggattt
     2101 cgttgcccgc gccgcgcgga agattggtta agaggtcgaa aatgcgcggg gtgaggatgt
     2161 agcgtccaac aacggcaagg ttggagggcg cgtcttcggg cttgggtttt tcgacaatgc
     2221 cggtaatgcg ttggaactgt ttgagctgtt cggtttcgac gatgccgtat gagccggttt
     2281 gcgatgcttc aacggtttct acgcccaaaa tgctgttgcc gctgcgcccg tacacttcga
     2341 ccatttgttt gagcgcgcct ttgggggcat caatcaggtc gtcggcaagg ataacggcaa
     2401 agggttcgtc tccgatggcg gcgcgggcgc acaagacggc gtgtcccaag cccagtgctt
     2461 ccgcctgacg gatgtagagg caggtaatgt tcggcggcag gatgttgcgg acgtgttcca
     2521 acaatttgtc tttatggcgc atttccaact cggtttcgag ttcgtatgcc ttgtcgaaat
     2581 ggtcttcgat gctgcgtttg ttgcgtccgg taacaaacac catttccgtg cagccggctt
     2641 ccacggcttc ttctacggcg tattggatca gcggcttgtc gacgatgggc agcatttctt
     2701 tcgggctggc cttggtggcg ggcaggaagc gggttcccat ccctgcgacg gggaaaacgg
     2761 ctttccgtat gggtttcatt ctttttcctt tgtattgttt tgatgtttaa agggcgagtt
     2821 tgcgtaagag ttcggcaagt gcctgcgcgc ggtggctttc gcggtttttg acctccgtat
     2881 ccaattcggc ggcggttttg ccgtgttcgg gcagataaaa atacgggtcg taaccgaaac
     2941 cgttttgccc gagcggcgtg tcgttccact gcccgtgcca tacgccctcg gcgataatcg
     3001 ggcgcgggtc gtctttatgg cggacaaaaa ccaatacgca gacatagcag cagcttttgt
     3061 ctgccttgcc gacaagttcg gcggcaagtt tcaggttgtt ggcggtatcg gatttgggat
     3121 tgtcgcccgc gtaacgtgcg gaatggatgc ccggcgcgcc gtttaaggcg gcggcacaga
     3181 tgccgctgtc gtcggcgagt gcgggcagcc cgctgtattt ggcggcatgg cgtgcttttg
     3241 ccagcgcgtt ttcgacaaag gtgggatagg gttcggggca ttcgggtatg ccgaatgcgg
     3301 attgcggcaa tacggtgatg ctgtaaggtt tgaataagtt gccgaactct tcgagcttgc
     3361 cggcattgcc gcttgccaaa acgatttttt ccggtttttc agacatagcg gttttccttt
     3421 gtggcggatt gggcggcgtg tagggatttg tgccgcaaat agagggcgag tgcgccgatt
     3481 tgcccgaaca gcgcgccgaa tgcgaaaaga aaggaggcga cggcgaaggc tttgctgccg
     3541 acggtcagta gataagcacc caagaggacg agcagcataa atgccagtgt gaacagggca
     3601 acagacaaca gatagatttt tcggcggttc atggcgttcg gtcggaaacg gtatgttcgg
     3661 attatatccg attgggacgg cattccctag agcttggaaa aatgccgtct gaaggggcgt
     3721 tcagacggca tggcgggcgt tatgcctgtt tgttccagcg ttcgatggat tctttgatga
     3781 cttttttcgc ttcttccaca tcgccccagg attcgacttt ggtcgtacct gcttttttca
     3841 ggtctttgta atggttgaag tggaactcga tttgtttgat gagctgttgc ggcaaatcgg
     3901 acaaagtttt gtaggcgttg ccgttgttgc ggtcgtcggc aggaacgcag acgattttgt
     3961 cgtccacttc gccgtcgtca acgaatttca tcacgccgat aacgcgcgct tccaagaata
     4021 cgccggttgc cagaggctgt tcggtaacga gcagcacgtc caattcgtcg ccgtcttcgt
     4081 ccaaagtttg gggaatgaag ccgtagttgg tcggtttggc gaagatggcg ggttcgacgc
     4141 ggtcgagttg gaatgcggcg agtttgcggt tccactcgat tttgtggttg ctgccggcgg
     4201 ggatttcgtt gacgacgttg atgatgccgc cgtccacatc gccgggggtc aggatttggt
     4261 tgaagtctgc catttggttt cctttgtttg ggaaagtttg aagtttgaaa gtatagcaca
     4321 aacgtccggc tgaaaatgcg cccgatgccg tctgaaaggg tgtacgggcg cgtgttaccg
     4381 tttgcccaaa aacctgccca gttccaaaat tgcgcgcctg ttggacgggg aaaacacttt
     4441 ttccgccgct tcttccaagc cgaaccagcc gtaggagacg tgttcctcgg gttgcaggac
     4501 gacgggcgta tcgcgcggga tttcggcaga gaagaggtgt tcgcggtttt caaacacgcc
     4561 ttttggatag cggtgccgcc agtggtggta gatttcgtaa accgtgctgt cgtgccagtc
     4621 ttgaagctgc ccgtccgcca gcaggatgcc ggtttcttcc caaacttcgc gccttgccgt
     4681 ttgggcgacg gtttcgcctg gttcgaggct gccggttacc gactgccaaa atcctttcgg
     4741 atgcgtgcgt tcgatgagca ggatgccgcc gtccccgcta taaaggacga ccagtgcgga
     4801 aacggggtat ttgagcggtt ttgccatcgg catctttcgg cgggctgcgg taatgaaggg
     4861 gcggattata gcaaacgccg cacgttatgg cgtttatcct tttccgtatc cttttttctg
     4921 cacggatggg acgcgccggt gtttgccggt aaattttccg ttgtgtcaaa aagataaggg
     4981 cggttgtgat tttaatgctt gccaaagcgt cgggcggaaa ctataatccg aaacttatcg
     5041 agtcggagtg tggcgcagtc tggtagcgca cttgcatggg gtgcaagggg tcgaaggttc
     5101 gaatcctttc actccgacca aaaattccga aagccgcttt caaaagcggc ttttttgccg
     5161 tccgtatgat tatgatgtag agtacgcggc gacagacatt caaatgccgt ctgacaaacc
     5221 gttcagatgg catctcttta tcttagtttc attccgtacc atcttaagga acatcaaatt
     5281 gggcatttcc cgcaaaatat cccttattct gtccatactg gcagtgtgcc tgccgatgca
     5341 tgcacacgcc tcagatttgg caaacgattc ttttatccgg caggttctcg accgtcagca
     5401 tttcgaaccc gacgggaaat accacctatt cggcagcagg ggggaacttg ccgagcgcag
     5461 cggtcatatc ggattgggaa acatacaaag ccatcagttg ggcaacctga tgattcaaca
     5521 ggcggccatt aaaggaaata tcggctacat tgtccgcttt tccgatcacg ggcacgaagt
     5581 ccattccccc ttcgacaacc atgcctcaca ttccgattct gatgaagccg gtagtcccgt
     5641 tgacggattc agcctttacc gcatccattg ggacggatac gaacaccatc ccgccgacgg
     5701 ctacgacggg ccacagggcg gcggctatcc cgctcccaaa ggcgcgaggg atatatacag
     5761 ctacgacata aaaggcgttg cccaaaatat ccgcctcaac ctgaccgaca accgcagcac
     5821 cggacaacgg cttgccgacc gtttccacaa tgccggcgct atgctgacgc aaggagtagg
     5881 cgacggattc aaacgcgcca cccgatacag ccccgagctg gacagatcgg gcaatgccgc
     5941 cgaagctttc aacggcactg cagatatcgt caaaaacatc atcggcgcgg caggagaaat
     6001 tgtcggcgca ggcgatgccg tgcagggtat aagcgaaggc tcaaacattg ctgtcatgca
     6061 cggcttgggt ctgctttcca ccgaaaacaa gatggcgcgc atcaacgatt tggcagatat
     6121 ggcgcaactc aaagactatg ccgcagcagc catccgcgat tgggcagtcc aaaaccccaa
     6181 tgccgcacaa ggcatagaag ccgtcagcaa tatctttatg gcagccatcc ccatcaaagg
     6241 gattggagct gttcggggaa aatacggctt gggcggcatc acggcacatc ctatcaagcg
     6301 gtcgcagatg ggcgcgatcg cattgccgaa agggaaatcc gccgtcagcg acaattttgc
     6361 cgatgcggca tacgccaaat acccgtcccc ttaccattcc cgaaatatcc gttcaaactt
     6421 ggagcagcgt tacggcaaag aaaacatcac ctcctcaacc gtgccgccgt caaacggcaa
     6481 aaatgtcaaa ctggcagacc aacgccaccc gaagacaggc gtaccgtttg acggtaaagg
     6541 gtttccgaat tttgagaagc acgtgaaata tgatacgaag ctcgatattc aagaattatc
     6601 ggggggcggt atacctaagg ctaagcctgt gtttgatgcg aaaccgagat gggaggttga
     6661 taggaagctt aataaattga caactcgtga gcaggtggag aaaaatgttc aggaaacgag
     6721 aagaaggagt cagagtagtc agtttaaagc ccatacgcaa cgagaatggg aaaataaaac
     6781 agggttagat tttaatcatt ttataggtgg tgatatcaat aagaaaggca cagtaacagg
     6841 agggcatagt ctaacccgtg gtgatgtacg ggtgatacaa caaacctcgg cacctgataa
     6901 acatggggtt tatcaagcga cagtggaaat taaaaagcct gatggaagtt gggaaatgaa
     6961 aaaaggaagg ggcggaaaac ctatgacaaa tcattctatg ttcccaaaag attgggatga
     7021 ggctagaatt agggctgaag ttacttcggc ttgggagaaa agaaaggaat tagaaggtca
     7081 aaaatggaga ggtacaagta aatctggtgt tgaaatagaa ggatttacgg aacctaatcg
     7141 aacagcttat ccactttata atagtaaatg aagtattgat taaaaaggtg tttttaaatg
     7201 tataaaataa aatgttttta tgatcagttt agttctggtg aattatttaa ctactgccag
     7261 tttttagata attcttctaa tcaaaagata aatacacgag gaacggtgta tcaatatatt
     7321 atttatgttt taacaggaga cctttatcta caaaaagaca tagatgaaaa tttagaattt
     7381 atccatcaag cagaaaataa tcccaatgaa gtttattcag gaggtggaca aggtttttgc
     7441 tgggatattt ctgctgaaaa agtagttttc tatcacaacg aatttgatga agaagacggc
     7501 tggcctgatt taagctgttc gctgcatact tttaaaactg ctttaattgc ttggaatgct
     7561 tttttgcaat tgcctaaaag tattcattcg gtggtggaga ctgtgattga ggaataagca
     7621 taattagctt aatgaataga atcagcgata tagattggac tgcaaatcca cgcttatacg
     7681 ctgtgccatg attaacagga agagagcaac ttggatgggc tagtagggat ggtaagcatt
     7741 taaatatatc aattgatgga aagattacac acaaatgact aatttaaaat tagatttcta
     7801 ctctgaagtt attataaaag attcttgccc taatgatttg ttagaaaatg gtgagactat
     7861 taaaggaaaa aaaggagtgg tattaggtat aagcgaagaa gatggtataa tctatggcta
     7921 tactatttta ctttttgata taaaatattg tatatatata gataagaaat atattatacc
     7981 gactggaaaa aaattctctc gcgatgattt ttattagcta atttaagtaa tttaagcaag
     8041 ttttttagat actgaaactg gtaattatgt ttatgtaagc ataaggagtt cagattgaag
     8101 gattctacag ggcaaatgag gctggcaacc aaggatttgg cggaagccat taaacgagga
     8161 gaagtacgta gttctgcttt tacaacaaag caactaaagg caatcgaaaa aggagaatct
     8221 aaaataccgg attatacttg gcatcatcat caggatactg gacgtatgca gcttgtacct
     8281 gaatgggaac attctaaaac cggtcacata ggagggacgg caatggataa aggtaaatga
     8341 ttatgtggaa aatttataaa gaaaatagca ccgacttaaa ttttgccata ggcagtatat
     8401 attgccaagc aatcaatctt accgaattta aaatgtgggt cgaaaaaatc ataagggaag
     8461 tggatttaga tgaaattcca aattattttt ttgatttgat agattttcaa tcactatacg
     8521 atctgattga tattatagga tttgttcccg aaaataattt atcaaaaaat caggataatg
     8581 cattaacagg tattgctttc ttaagaggga tagatgtcta tgaccctccc gtttcaaaag
     8641 aaaaagcatt aaaagcctta aagaaacatc ctgaaattta tcagaggttt cagcatttct
     8701 tcccatttgt agaactgccc ccgctttaaa cagtcaaaat gccgtctgaa acgatattcg
     8761 gcgttcagac ggcatttttt gatacaaagc aggtaaccaa aagagtgttt gacggcaaag
     8821 gaagataatt atgtggaaaa tcataaaaga agatagtgat gatttagaat ttgcaattaa
     8881 atgcttattc tctcaatcta ttgatttaaa tgaattcaag ttatggattg aacaagtaat
     8941 acgagatatg cccatcgagg acatcccttt ttatattttt gacttggcgg attttgatgg
     9001 gggaattgcc gatattgaca atattgtagg ttttgtttcg agttacagcc tatcaaaatc
     9061 gaaaaaaaat gccttgacag gcattgcctt cttaaggggg atatatgtct atgatccgcc
     9121 tatttcaaaa gaaaaagcat taaaagcctt aaagaaacat cctgaaattt atcagaagtt
     9181 tcagcatttc tttccgtttg tagaactgcc cccgctttaa acagtcaaaa tgccgtctga
     9241 aatgatattc ggctttcaga cggcattttt gatataaagc ggataactaa aagagcgttt
     9301 gacagcaaag gaacatctga aataccggat tacacttggc atcatcatca agatacagga
     9361 tgtattggtt ggaaagcgat gaacaaaaga agataattat gtggaaaatt tataaagaaa
     9421 atagcactga cttaaatttt tccctaggta gtatatattg ccaagcaatc aatattaccg
     9481 aatttaaaat gtgggtcgaa aaaatcataa gggaaatgga cttagatgaa attccaaatt
     9541 atttttttga tctaacagat cttcaatcac tattccatct aattgatatt ataggatttg
     9601 ttcccgaaaa taacttatca aaaaatcagg ataatgcatt aacaggcatt gccttcttaa
     9661 gggggataga tgtctatgat tcgcctatct caaaagaaaa agcattaaaa gccttaaaga
     9721 aacatcctga aatttatcag aggtttcagc atttctttcc gtttgtagaa ctgcccccgc
     9781 tttaaacagt caaaataccg tctgaaagcc atttccgccg ctcagacggc attttttcct
     9841 ctttcgttta caaaccctta aaatccattt acactcaaac tcgttcaaaa caaaacaaac
     9901 cctgccatga aaaccctgct cctcctcatc cccctcgtcc taaccgcctg cggcacactg
     9961 accggcatac ccgcccacgg cggcggcaaa cgctttgccg tcgaacaaga actcgtcgcc
    10021 gcatcgtccc gcgccgccgt caaagaaatg gatttgtccg ccctgaaagg acgcaaagcc
    10081 gccctttacg tctccgttat gggcgaccaa ggttcgggca acataagcgg cggacgctac
    10141 tctatcgacg cactgatacg cggtggctac cacaacaacc ccgaaagtgc cacccgatac
    10201 agctaccccg cctacgacac taccgccacc accaaatccg acgcgctctc cagcgtaacc
    10261 acttccacat cgctcttaaa cgcccccgcc gccgccctga ctagaaacag cggacgcaaa
    10321 ggcgaacgct ccgccggact gtccgtcaac ggcatgggcg actaccgcaa cgaaaccctg
    10381 ctcgccaacc cccgcgacgt ttccttcctg accaacctca tccaaaccgt cttctacctg
    10441 cgcggcatcg aagtcgtacc gcccgaatac gccgacaccg acgtattcgt aaccgtcgac
    10501 gtattcggca ccgtccgcag ccgtaccgaa ctgcacctct acaacgccga aacccttaaa
    10561 gcccaaacca agctcgaata tttcgccgtc gaccgcgaca gccggaaact gctgattgcc
    10621 cctaaaaccg ccgcctacga atcccaatac caagaacaat acgccctctg gatgggacct
    10681 tacagcgtc
//
LOCUS       DAXHNE010000057        10605 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.057, whole genome shotgun sequence.
ACCESSION   DAXHNE010000057 DAXHNE010000000
VERSION     DAXHNE010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10605
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.057"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(16..1218)
                     /gene="trpB"
                     /locus_tag="ACFM4Z_001110"
     CDS             complement(16..1218)
                     /gene="trpB"
                     /locus_tag="ACFM4Z_001110"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFC6021156.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALQELADAYKAAKNDPE
                     FWEAFRHDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(1301..1990)
                     /locus_tag="ACFM4Z_001111"
     CDS             complement(1301..1990)
                     /locus_tag="ACFM4Z_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC6021157.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLHERGIASCPTVLKAVRQHGNASDKFLKVVETVDLAAVLAKIPECRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAF
                     FEMAGLCEKQL"
     gene            2187..2966
                     /gene="rsmA"
                     /locus_tag="ACFM4Z_001112"
     CDS             2187..2966
                     /gene="rsmA"
                     /locus_tag="ACFM4Z_001112"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFC6021158.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            3139..3867
                     /locus_tag="ACFM4Z_001113"
     CDS             3139..3867
                     /locus_tag="ACFM4Z_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC6021159.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVLFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            3940..4299
                     /locus_tag="ACFM4Z_001114"
     CDS             3940..4299
                     /locus_tag="ACFM4Z_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021160.1"
                     /translation="MYFVDRTAVVLKPTARFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEAELNITPVFDNMM"
     gene            4304..4744
                     /locus_tag="ACFM4Z_001115"
     CDS             4304..4744
                     /locus_tag="ACFM4Z_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFC6021161.1"
                     /translation="MRAFQTALRPSRILNILTVSLHAASLAVCLTWFYGWMMWFGLAA
                     LVASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGTTVSQCIIPDMTDKKSYRRLKVWILWRQPKKTAETDTPS"
     gene            4776..6050
                     /gene="folC"
                     /locus_tag="ACFM4Z_001116"
     CDS             4776..6050
                     /gene="folC"
                     /locus_tag="ACFM4Z_001116"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFC6021162.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARSEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPESLLSHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDDPARNRNALPFPALRGTYQLSNAACTLTVLECLNAKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            6063..7061
                     /gene="ftsN"
                     /locus_tag="ACFM4Z_001117"
     CDS             6063..7061
                     /gene="ftsN"
                     /locus_tag="ACFM4Z_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC6021163.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEYSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAVGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            7054..7551
                     /locus_tag="ACFM4Z_001118"
     CDS             7054..7551
                     /locus_tag="ACFM4Z_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFC6021164.1"
                     /translation="MNNLPVADLLVFAIIAACIVLSAMRGVIAEAGSMAAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGVLIVTLLVMLASKTDLPDTEEWQQSYTIPFFVSLSEAVLNHSGGTAE
                     TPEDD"
     gene            7759..9303
                     /gene="purF"
                     /locus_tag="ACFM4Z_001119"
     CDS             7759..9303
                     /gene="purF"
                     /locus_tag="ACFM4Z_001119"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFC6021165.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLVGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFDGTIIARQCSDKAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLKKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            9403..9894
                     /gene="greB"
                     /locus_tag="ACFM4Z_001120"
     CDS             9403..9894
                     /gene="greB"
                     /locus_tag="ACFM4Z_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFC6021166.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            9953..10579
                     /locus_tag="ACFM4Z_001121"
     CDS             9953..10579
                     /locus_tag="ACFM4Z_001121"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFC6021167.1"
                     /translation="MRKIRTKICGITTPEDASAAVAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDTFCRQFHRPYIK
                     AIRVQTTSDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDAAKVAAFIATANRLFR"
BASE COUNT         2639 a         3054 c         2755 g         2157 t
ORIGIN      
        1 atcagacgga cgaggttaca gtttaatccc tttgagcttc gcgacggtat tgatgtcttt
       61 gtcgccacga cctgagaggt tgaccaaaat cacttggtct ttacccattt tcggcgcatt
      121 tttcaccgcc caagcaacgg cgtggctgga ttcgagcgcg gggatgatgc cctcgaagcg
      181 gcagagcaag tcaaaggctt cgagtgcttc gtcgtctttg gcaacagtgt attcgacgcg
      241 cttgatgtcg tgcagatggc tgtgttccgg gccgatgccg gggtaatcca agcctgcgga
      301 aacagagtgc gtacccaaaa cctgaccgtt ttcgtcctgc atcagatagc tgcggaaacc
      361 gtgcaatacg ccaatcggtg cgcccgaagt aatcggcgcg gcgtgatcgg gggtgttcac
      421 gcccaaaccg ccagcctcca cgccgacgag gcgcacgttt tcttcgccga tatacgggtg
      481 gaacaaaccg atggcgttcg atccgccgcc cacgcaggca acggcaacgt cgggctgtct
      541 gccgatggct tcctgcatct gcgctttagc ttcgttgcca atcacgcatt ggaaatcgcg
      601 caccatttcg ggatacggcg cggggccggc ggcggtaccg atgatgtaga acgtgtcgtc
      661 cacgcgggcg acccattcgc gcatggcttc gttcatcgcg tctttcagcg tgcggctgcc
      721 gctttctaca ccgaccacgt tcgcacccaa taatttcata cggaacacgt tgggcatttg
      781 gcgttggatg tcgtccgcgc ccatatacac gtcgcaagtc ataccgaagc gtgcggcaac
      841 ggtggcactc gccacgccgt gctgacccgc gccggtttcg gcgatgacgc gttttttacc
      901 catgcgtttt gccaacagtg cctgaccgat ggtgttgttg actttgtgcg cgccggtgtg
      961 gttcaagtct tcgcgcttca accagatttg cgcgccgccc agatgttcgg acaaccgcgc
     1021 ggcgtggtaa acggggctgg gcctgccgac ataatgtttc aaatcatggc ggaacgcttc
     1081 ccaaaattca ggatcgtttt tcgctgcctt ataggcatcc gccagctctt gcaaggcggg
     1141 aatcagggtt tcggagacat aaagcccgcc gtgttcgccg aaaaagccct tctcgtcggg
     1201 cgcgtggtag ttcttcatcg gattcttcct tgtgcttcaa attttcagac ggcatttccg
     1261 tttttgcaaa catgccgtct gaacgggaaa ttgtcggcaa ttataactgt ttttcacaca
     1321 agcccgccat ttcaaaaaac gcccgatacg ccgccgcttt tttcgccaaa ctcaaaggat
     1381 aggcgcgcga agtcgaaggc aggcgcgtca gcgtcaaatc ccgtccggca aacggaaacg
     1441 gcacggtttc gcccgttttc ggcattttga taccgccgcc ctgaatatcg agcaggattt
     1501 ccgtcgcctt gccgcctgtc gtacaaatat ggcggcactc gggtattttt gccaacaccg
     1561 ccgccaaatc gacggtttca actaccttta aaaacttgtc ggacgcattg ccgtgctgac
     1621 gtaccgcctt caaaacggtc ggacaggacg caatcccccg ttcgtgcaaa aacgccttga
     1681 ttttctcggc atcaaacgct ttttcagaca acctttggaa atgcgccgca tcattaaaaa
     1741 acaccagccc gtaaacgcgc cacatatcgt tttggaaatt cggataatga aactgcatcg
     1801 cgcgtttgtc ttccttgggc ggaaacgtcc ccatcatcat gaccgcagcc ttcggcggca
     1861 acacggaatc gaagggatgg gtttcgattt caagcagatt ttcagacacg ttcaatcctc
     1921 caacaggcaa aacggcagac agccggtttt tgcaccaaac cgccaaacac ggcaagcctt
     1981 caaacagcat tatcacgccc tgccgttttc ggttacaatg ccgacatcaa acggatactg
     2041 taaacacatt tatgtttcaa cacacaggac gacacataaa gcaccgccct atgtgccgtc
     2101 ctgatttgga aggagttacg cccctcccaa atagagtctg attctaccgc cctaaagggc
     2161 ggggtttcaa ccgaaaagga aacacgatga aagaacacaa agcccgcaag cgtttcgggc
     2221 agaatttttt gcaggacacg cggattatca gcgatattgt caacgccgtg cgcccgcagg
     2281 cggatgatgt cgtgattgaa atcggtccgg gtttggcggc gattaccgaa cctttggcga
     2341 aaaagctgaa ccgcctgcac gttgtcgaaa tcgaccgcga catcgtatgc cgtctgaaaa
     2401 cgctgccgtt tgcggataaa ctggtgattc acgaaggcga tgtattgcag tttgatttca
     2461 acggcatcgc aggcaaaaag aaaatcgtcg gcaacctgcc ctacaacatt tccacgccgc
     2521 ttttgttcaa gctggcggag gtggcggacg atgtcgtcga tatgcacttt atgctgcaaa
     2581 aagaagtggt cgagcgtatg gttgccgcgc cgaaaagcaa cgactacggc cgcttgggcg
     2641 tgatgctgca atattttttc gatatggaaa tgctgattga cgtaccgccc gaatcgttcg
     2701 accctgcgcc gaaagtggat tccgccgtcg tccgtatgat tccggtgaaa caccgcatcg
     2761 gcaaggcgga cgatttcgag catttcgcca aactggtgaa actcgccttc caccaacgcc
     2821 gcaaaaccat acgcaacaac ctgaaagagc ttgcaggcga cgatgatttg caggctgtcg
     2881 gcatcaatcc gcaagaccgt gccgaacaca tcgcgccgga aaaatacgta gcgttgagca
     2941 attatctggc gggcaaggcc gtctgaaaag ggagtaagcc gatgaagaaa tacctgcttt
     3001 tacccctgat actgccgctt gcagcctgcc tgaccaatcc gaacgaccgt ttttttgacg
     3061 gcagacgcta cacgcttcat catatggagc tcccgtaacg acaacctgca acggcaaatg
     3121 ccgtctgaaa gaaaagacat gatcaaattc aaaaacgtac acaaacattt caaagacctg
     3181 cacgtcatca acggcgtgaa cttggaaatc aaaaaaggcg aagtggtcgt cgtctgcggg
     3241 ccttcgggca gcggcaaatc cacactcatc cgcaccgtca accagcttga aagcatcgaa
     3301 agcggcgaga tttgggtgga cggtgtcaac gttgccgacc caaaaaccga tttgaacaaa
     3361 atccgcgaag aagtcggctt tgtgtttcaa agtttcaacc tctacccgca cctgaccgtg
     3421 ttggaaaaca ttaccctcgc accgatgaag gttaaggggc aaaatgcgga acaggcggag
     3481 aaaaaggcaa tggagctttt ggaacgcgtc ggactggcac acaaaaaaga cgccttcccg
     3541 tcccaacttt ccggcggcca gcagcaacgc gtggcgattg cgcgcggttt ggcgatggaa
     3601 ccgcgcgtaa tgttgtttga cgaaccgacc tctgcactcg accccgaaat ggtcggtgaa
     3661 gtgttgaaag tgatgaaaga cttggcggaa agcggcatga cgatgatgtg cgtaacccac
     3721 gaaatgggat ttgcccgcga agttgccgac cgggtacttt ttgtcgataa agggcaaatc
     3781 ctcgaagacg aaacgcccga agcgtttttt accaacccaa aacacgaacg cgccaagcag
     3841 ttcctgcagc aggtcatgac ccactgatgc tatgccgtct gaaaaccgaa cgttcagacg
     3901 gcatagcccg acaccgcaag atgattgaga aagaaaacca tgtattttgt tgaccgcacc
     3961 gccgtcgtcc tcaagccgac cgcgcgcttt ttggaatggc tcaaaagcac cgacgaaaat
     4021 atgcccgacc tgaccataga acagcttcgt gccaactgtt ccgtcttcct cgttccgcag
     4081 ttcgacgaac cggaagccgt cgtttcctat ttcgacgaac gctaccgcca gattttcgag
     4141 gcggagctgg caggttggga catcgataag gataaatggc cgcaagatat ggggctgaag
     4201 gcgttttggg aatttttcga catcgaaatc cacgatatgg tgttggacat ggaagaagcg
     4261 gaattgaaca ttacccccgt gttcgacaac atgatgtaag gctgtgcgcg cctttcagac
     4321 ggcattgagg ccgtcccgca tcctgaacat cctgactgta tcgcttcacg ccgcctccct
     4381 tgccgtctgc ctgacttggt tttacggctg gatgatgtgg ttcggtttgg cggctttggt
     4441 ggcaagttac gcatactcgc tcaggataac gaatctgaaa caccgccatg ccataaccgc
     4501 cattaccata gaccgcgacg gacaggcgga aatcgtatcc ggcaaagaca aaacagcggc
     4561 aacacttgcg ggcagcagta tggttacgcc ttatgcctta ttcttacaat gggacacagg
     4621 cggcacaacc gtcagccaat gcatcatacc cgacatgacg gacaaaaaat cctaccggcg
     4681 gctgaaagtc tggatacttt ggcggcagcc gaaaaaaacc gccgaaaccg atacaccaag
     4741 ctgatttcag acggcatttg aacacggaac actcaatgaa aacactgcaa gactggctct
     4801 cgcatttgga aaccgcccac agcggcggct tgatcgatat gggcttggaa cgcgtaggcg
     4861 aagtgaaaaa acggatgaag ctcacgccgc aatgccccgt cgtcgttgtc gcgggaacaa
     4921 acggcaaagg ttcggtctgc gcctacctga cacagattta caaacaggcg ggctacaaaa
     4981 ccggcacact gaccagcccg catttattgc gttacaacga acgcatcgcc atcaacgccg
     5041 aacccgtttc ggacgatacg attattgcct ctttcgagcg catcgaagcg gcgcgcagcg
     5101 aaatatcgct gacttatttt gaattcaata cgttggcggc cgtcgacatc ttcatgcgcg
     5161 aacaggttga cgtgatgata ttggaagtcg gcttgggcgg acgcttggac gcggtcaacg
     5221 catttgacgg cgattgcgcg gtggttacta gtgtagattt ggaccatcag gcgtttttgg
     5281 gcgatacggt cgagcaggtc ggctttgaaa aagcaggcgt gttccgcagc ggcaaacccg
     5341 caatctgcgg tcaaaacccc gcgcccgaat cgttgctttc acacgccgaa gccataggcg
     5401 cgaaactgct gatggtgcag cgcgatttcg agtttcacgc gatggaaaac atccaatgga
     5461 actaccgctt ccggccgcaa cattcagacg accccgcgcg caaccgcaat gccctgccct
     5521 tccccgcatt gcgcggcaca taccaacttt ccaacgccgc ctgcacgttg accgtattgg
     5581 aatgcttgaa cgccaaattg ccggtggaca tcggcgccat caaacgcgga ttgctgctgg
     5641 ttgaaaatcc cggacgcttc caagtcctgc ccggccgtcc gctgaccgtt ttggatgtcg
     5701 gacacaatcc ccacgctgcc cgcgccctgc gccgcagcct gattaatttg gcgtttgcgc
     5761 aaaaacgcac cgccgtattc agtatgctgt ccgacaaaga catagacggc gtgttggaaa
     5821 tcgttaaaga ccagtttgac gagtggtata tcgcaccgtt ggatgtaccg cgcggcatga
     5881 cgctcgagag cctgcaaaac aaactgcacg aacacagcat cgaaaacata cagacattcg
     5941 ccgccgtccg cgaagcatac cgggcggcag cctcaaaagc gggcgagaac gacagaatcg
     6001 tcgttttcgg ctcatttcat accgttgccg atgtcatgtc ggtactttaa aaggaaactt
     6061 ctatgtccga aaacaaacaa aacgaagtcc tgagcggtta cgaacaactc aaacggcgca
     6121 accgccgccg cctcgtaacg gcaagttgcc tggttgccgc ctcctgcatc ctgctggcag
     6181 ccgccctcag ttccggccct gccgaacaga ctgccggcga aacaagcggc gtagaaaaca
     6241 aagcggcagg tgcggcacaa acccctgcct tgaaatccgc cgccgacaaa ccgcaggact
     6301 tggcaggcga agacaagcct tctgccgccg acagcgaaat cagcgagcct gaaaacgtag
     6361 gcgcgccgct ggtgctgatt aacgaccgcc tcgaatacag caacatcaaa ggtttggaag
     6421 catccgagaa actgcaacag gcagaaaccg ccaaaaccgc accgaagcag gcaaaacaac
     6481 gcgctgccga aaaagtgccg gcaactgccg acagtacgga tacggtagcg gttgaaaaac
     6541 cgaaacgcac tgccgaaaca aaaccgcaaa aagcggaacg cactgccaaa gccaagccca
     6601 aagccaaaga aaccaaaacc gccgaaaaag ttgccgacaa accgaaaact gccgccgaaa
     6661 aaaccaaacc ggatacggca aaatccgaca gcgcggtaaa agaagcgaaa aaagccgaca
     6721 aggctgaaag caaaaaaaca gccgaaaaag accgttcgga cggcaaaaaa cacgaaacgg
     6781 cacaaaaaac cgacaaagcg gacaagacca aaaccgccga gaaggaaaaa tccggtaaaa
     6841 aagccgccat tcaggcaggt tatgccgaaa aagaacgcgc cttaagcctc cagcgcaaaa
     6901 tgaaggcggt gggtatcgat tcgaccatca ccgaaattat gaccgacaac ggcaaagttt
     6961 accgcgtcaa atcaagcaac tataaaaacg caagggatgc cgaacgcgat ttgaacaaat
     7021 tgcgtgtaca cggtatcgcc ggtcaggtaa cgaatgaata acctccccgt tgccgacctc
     7081 ctcgtcttcg ccatcatcgc cgcctgcatc gtgctatccg cgatgcgcgg cgtgattgcg
     7141 gaggcaggtt cgatggcggc atgggtggtt tccttctttt ttgccaaact ctttgccgca
     7201 cccttcgccg acctcgcctt tgcctcgttc caaccccgcc tgtttgcatt ggctctgtcg
     7261 ttcatttccc tgttcgtcat tgcctgtctg atccagaaaa tgctccgttc gctgctgacc
     7321 ggcgcagttt cggcggtcgg tttgggcttt gccaaccgca ttttgggcgg cgtattcggt
     7381 gcattgaaag gcgttttgat tgttaccctg ctggtcatgc ttgcttcaaa aaccgacctg
     7441 cccgataccg aagagtggca gcagtcctat accataccgt ttttcgtatc gctttccgaa
     7501 gccgtgttga accatagcgg cggcacggcg gaaactccgg aagacgattg attgccatac
     7561 ggatatttca gacggcattc tcttttatgc cgtctgaaat gttttttcct ttcctcagcc
     7621 atatcacaga ttaaatctta tcctccataa acccacctat ctgtccttcg ctcaaatatt
     7681 gtcaacaatt cgggcaaaaa gccgccgttt gctttataat tccgttttac ccttaaacct
     7741 cgaatcggag aagacgatat gtgcggcgta ttaggtttgg tcagtcatga acccgtgaac
     7801 cagcttctgt acgacggttt gcagatgttg cagcacaggg ggcaggatgc ggcgggcatt
     7861 gcaacggcgg aaggcggcac cttccatatg cacaaaggca aagggatggt gcgcgaagtg
     7921 ttccgcacgc gcaatatgcg cgatttggtc ggcaacgccg gcatcgccca cgtccgttat
     7981 cccacggcgg gcaacgccgg cagcagcgcg gaggcgcagc ctttctacgt cagctcgccg
     8041 ttcggcatcg ttttggcgca caacggcaac ctcaccaaca ctgccgaact gtatgaaaac
     8101 gtgtgcaaca aacacctgcg ccacgtcaac accagctccg attccgaagt cttgctcaac
     8161 gtattcgcgc acgaattacg ccgcgaagtc tctaaaaacg ccgacccgca ccggctcaat
     8221 gccgacaata ttttcaacgc cgttgccgaa gtccaccgcc tggtgcgcgg cgcatacggc
     8281 gtggttgcca tgattgcggg ctacggtatg cttgccttcc gcgaccctta cggcatccgc
     8341 ccgctggtat tgggttcgca aaccgacagc gaaggcagga aatcctatgc cgtcgcctcc
     8401 gaatccgtcg ccttcaacgc gcttgcctac gatttggaac gcgatatccg gccgggcgaa
     8461 gcggtatttg tcggctttga cggcacaatc atcgcccgac aatgcagcga taaggccaaa
     8521 ctcagcccct gcctttttga atacgtctat tttgcccgtc ccgactccgt gattgacggc
     8581 gtgtccgttt accagtcgcg cttggatatg ggcgtgtcct tggcggaaaa aatcaaacgc
     8641 gagctgcccg tggacggcat cgatgtcgtg atgcccatcc ccgacaccag ccgccccagc
     8701 gcgatggagc ttgctgtcca cctcaaaaag ccttaccgcg aaggtttgat taaaaaccgc
     8761 tatatcggcc gcacctttat tatgcccggc caggcgacgc gcaaaaaatc cgtgcgccaa
     8821 aaactcagcc cgatggaaac cgaatttgca ggcaaaagcg tgttgctggt ggacgactcc
     8881 atcgtgcgcg gtacgaccag ccgtgaaatc gtcgaaatgg tacgcgcggc tggcgcgcgc
     8941 aaagtctata tcgcctccgc cgcgcccgaa gtgcgctatc ccaacgtata cggcatcgat
     9001 atgcccacgc gcgaagagtt gattgccaat gggcgcagcg cggcggaaat cgccgccgaa
     9061 atcggcgcgg acggcatcgt gtttcaagac ttgggcgatt tggaagccgt cgtcaaagca
     9121 ctcaacccga aaatcgaatc cttcgattcg tcctgtttca acggcattta tcagaccggc
     9181 gacatcgatg atgcctacct cgaccgcctg tctgccgaga aatccggctg cggcggtctg
     9241 aaaatccacc cgagcaggat ggaacacagc atcagcatca gcgatgcggg cgacgaagaa
     9301 taaggcttcc caaaccgcga tgccgtctga aatccgaacg ccgggttttc agacggcatt
     9361 tcccatatcg gcatcacatt tcccgtttca tccgcacaca atatgagtac cgaaaccaaa
     9421 aactacatta cgcccgccgg atggcaggca ttgaaagacg agctttatca actggtcaac
     9481 aaagaacgcc ccgaaatcgt ccaaatcgtc aactgggcgg caggcaacgg cgaccgcagc
     9541 gaaaacggcg actatcttta cggcaaacgc cgtatgcgcg aaatcgaccg ccgcatccgt
     9601 ttcctgacca agcgtttgga agccgccgtc gtcgttgatc ccgaattgcg cgaagcgacc
     9661 gaccaagtgt ttttcggcgc gaccgtcggt ctgttgcgtg gcgacgggcg cgagcaaacc
     9721 gtcaaaatcg tcggcatcga tgaaatcgat accgcgcaaa acaaaatttc ttggatttcg
     9781 ccgctggctc gttgtctgat taaagcgcgc gaaggcgacg aagtggtttt gaacacgccg
     9841 gaagggcgtg aggaaattga aatcctttct gtcgaataca tcaaaatcga ctgatcggga
     9901 atgccgtctg aaacggtgca agcgcgccgt ttcccaaata aaggaatacc gaatgaggaa
     9961 aatccgcacc aaaatctgcg gcatcaccac accggaagac gcatcggccg ccgtagcggc
    10021 aggtgcagat gccgtcgggc tggtcttttt ccaaggcagc agccgggccg tcgatattgc
    10081 ccgcgccaaa aaaatcaccg ccgcactgcc gccgtttgtc agcgttgtcg cccttttcgt
    10141 caacgaaagc gcgcaaaaca tccgccgcat ccttgccgaa gtgccgatac acatcatcca
    10201 attccacggc gacgaagacg acacattctg ccgtcagttc caccgcccct atatcaaggc
    10261 cattcgtgtt cagacgacat cagacatccg aaacgccgcc gaccgcttcc ccgacgctca
    10321 ggcactgctt ttcgatgcct accatccttc ggaatacggc ggcaccggac accgcttcga
    10381 ctggacgctg ctggcggaat attcgggcaa accgtgggta cttgccggcg ggctgacccc
    10441 tgaaaacgtc ggcgaagccg tccgcatcac cggagcggaa gcggtcgatg tatccggcgg
    10501 cgtggaagca tcgaaaggaa aaaaagatgc cgccaaagtt gccgccttta tcgcaaccgc
    10561 caaccgccta ttccgttaaa gcaacaaaaa ttgccgctgg aatga
//
LOCUS       DAXHNE010000058        10483 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.058, whole genome shotgun sequence.
ACCESSION   DAXHNE010000058 DAXHNE010000000
VERSION     DAXHNE010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10483
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.058"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(182..394)
                     /locus_tag="ACFM4Z_001122"
     CDS             complement(182..394)
                     /locus_tag="ACFM4Z_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFC6021168.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAKPD
                     TETRRTDMSLEKWRQFLDWTEKTNAV"
     gene            complement(406..687)
                     /locus_tag="ACFM4Z_001123"
     CDS             complement(406..687)
                     /locus_tag="ACFM4Z_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021169.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDSEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(725..928)
                     /locus_tag="ACFM4Z_001124"
     CDS             complement(725..928)
                     /locus_tag="ACFM4Z_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021170.1"
                     /translation="MNIIEQEMKHSSLRKVDAEIAKIIADAHKINAESVKIAQESRWY
                     PMMAATGLVTAIAAVLALIFKFA"
     gene            complement(1008..1580)
                     /locus_tag="ACFM4Z_001125"
     CDS             complement(1008..1580)
                     /locus_tag="ACFM4Z_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC6021171.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVL
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQNTCNKT"
     gene            complement(1555..1899)
                     /locus_tag="ACFM4Z_001126"
                     /pseudo
     CDS             complement(1555..1899)
                     /locus_tag="ACFM4Z_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1892..2767)
                     /locus_tag="ACFM4Z_001127"
     CDS             complement(1892..2767)
                     /locus_tag="ACFM4Z_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HFC6021172.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELAEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHGISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(2812..3102)
                     /locus_tag="ACFM4Z_001128"
     CDS             complement(2812..3102)
                     /locus_tag="ACFM4Z_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC6021173.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3114..3359)
                     /locus_tag="ACFM4Z_001129"
     CDS             complement(3114..3359)
                     /locus_tag="ACFM4Z_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC6021174.1"
                     /translation="MINHECINQPYGRILKFSRVQAKEMLTADELKENFAKNGQTLAQ
                     WARENGFKPRDVYLVIGGQRKGNYGKGHEIAKKLGLK"
     gene            complement(3352..3576)
                     /locus_tag="ACFM4Z_001130"
     CDS             complement(3352..3576)
                     /locus_tag="ACFM4Z_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC6021175.1"
                     /translation="MEKPLNFKPIPYPQTRESAAGWFKRNGVCKAHWAKYFNLERTTV
                     EHLLRGKLKGNFGKSHEAAVLLGMKEKSDD"
     gene            3848..4219
                     /locus_tag="ACFM4Z_001131"
     CDS             3848..4219
                     /locus_tag="ACFM4Z_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6021176.1"
                     /translation="MNNSSFFGNRLKEERKKLKMTQAEIAEKCGVSGRMWGDYERGIS
                     QPKAELFFQFEKVGIDVQYVMHGRRGETAVMPSETLNAEEQELLVLFREAAAADREMI
                     LMVARRAEKKAQTALGKVSNG"
     gene            4212..4844
                     /locus_tag="ACFM4Z_001132"
     CDS             4212..4844
                     /locus_tag="ACFM4Z_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021177.1"
                     /translation="MDKMDQFELCQKEHVNPFALSKQYLLVVTFVKSSSKNFQAALLW
                     ARSAKLFENLEIGKETIYCCAFDKTAEQAGMAGVFLNYIENWNGKQIYINGRIHSGSI
                     YDLLGVLDCYQKSQSCPNPKSHCCFVSDDIFLWHGSRPTFEISLDLTGKKKETSSAKK
                     FVMPCINFRHHRIEKETYLGNWNEQIAALAVKQNIDWCPSFDIENFRQYE"
     gene            4837..5031
                     /locus_tag="ACFM4Z_001133"
     CDS             4837..5031
                     /locus_tag="ACFM4Z_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6021178.1"
                     /translation="MNNYYLYRNCSSDVLWVKRIQRQIDGSLLLISDNSTYPPMPLAL
                     AEHPDIQIIGQVVQVSKDLN"
     gene            5055..5618
                     /locus_tag="ACFM4Z_001134"
     CDS             5055..5618
                     /locus_tag="ACFM4Z_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021179.1"
                     /translation="MKILALLIAATCALSACGSQSEEQPASAQPQEQAQSELKTMPVS
                     YTDYQSAANKGLNDQKTGLTLPEHVVPIDNAEGKNLLHDFSDGLTILTVDTDKADKIT
                     AVRVVWNTDAMPQKAEKLSKAAAALIAATAPEDRTMLRDTGDQIEMAIDSHNAQKEPT
                     REWARGGIAYKVTVTNLPSVVLTAKAE"
     gene            5649..6596
                     /locus_tag="ACFM4Z_001135"
     CDS             5649..6596
                     /locus_tag="ACFM4Z_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021180.1"
                     /translation="MMIEKSISIVDGKEYSVFAVSHEFRYTFDEPILVADLISSLKAY
                     ETLTSSYLPAILNQLFDVKIQKIKVAVSEIERGSFLEKLIFNLFFKDEDAYNEFCLKI
                     RKFLGTENQDGSINMSKIIMFAMTTLLGVGAGYLLFKNPPQEKQAITNNIVTVINADS
                     SVALDGEHLVSVVKEVTGSSKQKTAENVAKVYAPASKNNGSITLGTDDVRIEPVAQQT
                     VATLPKDVDLRDTPLTEDYTDIDVQIRATDRDKNSGWYAVIDQIVPSRVRLELPEDID
                     LNRLANNATIRANVTVEFDLKQNGSRKPKKIILTSLSTD"
     gene            6681..7184
                     /locus_tag="ACFM4Z_001136"
     CDS             6681..7184
                     /locus_tag="ACFM4Z_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HFC6021181.1"
                     /translation="MSDKFNQFINRVLSHEGGYANHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAISIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VPDDGVIGAVSLKAINSLPENDLLLRFNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     ASADNTD"
     gene            7198..7371
                     /locus_tag="ACFM4Z_001137"
     CDS             7198..7371
                     /locus_tag="ACFM4Z_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021182.1"
                     /translation="MSKKSLIALMTAAMQPDFSHSDLGIRYAMPTQGCWTQAHRKSGV
                     AAAKRAAKKKRHK"
     gene            7296..7628
                     /locus_tag="ACFM4Z_001138"
                     /pseudo
     CDS             7296..7628
                     /locus_tag="ACFM4Z_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7625..8035
                     /locus_tag="ACFM4Z_001139"
     CDS             7625..8035
                     /locus_tag="ACFM4Z_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HFC6021183.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAKQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLKAEMVALKKPDALIHGKEVRQKERNSSQG"
     gene            8068..8601
                     /locus_tag="ACFM4Z_001140"
     CDS             8068..8601
                     /locus_tag="ACFM4Z_001140"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFC6021184.1"
                     /translation="MSFYYQLAVQSAEDKYADLKRHIHDIYRRHKGRYGYRRIAAAIR
                     HAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNILRRCFHAEKPNEKWV
                     TDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQIRPTFDLAGEILKGAPEKPGSSEKPI
                     LHSDQGWQYQMFFIKSS"
     gene            8661..8843
                     /locus_tag="ACFM4Z_001141"
                     /pseudo
     CDS             8661..8843
                     /locus_tag="ACFM4Z_001141"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(9161..9523)
                     /gene="ssrA"
                     /locus_tag="ACFM4Z_001142"
     tmRNA           complement(9161..9523)
                     /gene="ssrA"
                     /locus_tag="ACFM4Z_001142"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(9586..10191)
                     /locus_tag="ACFM4Z_001143"
     CDS             complement(9586..10191)
                     /locus_tag="ACFM4Z_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFC6021185.1"
                     /translation="MNTDFQNILKQLGKQAKKEAAEKQEAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPSDKTPIKVRPKDNRADEENYFYIGSTYNEPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
BASE COUNT         2548 a         2448 c         2659 g         2828 t
ORIGIN      
        1 aacctgtgtt tgggtttcgg ctgtcgggag ggaaaggaat tttgcaaagg tctcagagtg
       61 agtttatttt ggggcggcgg caggtcgggg gcaagcggcg tggggcttgg ttgtggtttt
      121 taggtttggg ggtgggtaaa aaatgccgta cccggcgggc aggcttcgca ctctgaaggt
      181 ttcagacggc gtttgttttt tctgtccaat cgaggaactg ccgccatttt tccagcgaca
      241 tatcggtccg ccgcgtttcg gtatcgggtt tggcttccca gcggcctacc tgtatgtagt
      301 gcttcacccc gacgatttgc gccaactcgg cctgtgtcag tttgcagcgg ttgcgcaggg
      361 tgcgaaggtt gtaaggcgtg tagccgagtt ccatgtcgtt gcggtttatt ttgtttcgca
      421 tatttttttg actgcccggc ggcaggtttc ggtaaggacg gcggcaaatc gggcttcgtc
      481 tgccggtttt ccgtcgaaaa aaataaaatc gtaaagggtg atgccgtttt cggaatggcg
      541 gtaatgcccg cccgggcatt cgctgtctgt ctgcctgtgg attttggcaa tcaggcgggg
      601 atagcggcaa tgggtaatga aacggcttgc gccgtcttgc ccgataatcc attcggggta
      661 gcggttgaat agtgcggctc tgttcatttt gttcggggga taaagcccct cgcggggctt
      721 gtggtcaggc aaatttgaat atcagtgcca acacggcggc gatggcggta accagcccgg
      781 tggccgccat catgggatac catcgggatt cttgggctat ttttacggat tcggcgttga
      841 ttttgtgcgc gtctgcaatg attttggcga tttcggcatc tacttttctc agactggagt
      901 gtttcatttc ttgttcgatt atgttcatcg tacttccttt cgtttttggc ggttgccgcc
      961 gcttgtcgga tggtaggatg tctgccatgt gtatatattg atacctttta ggttttattg
     1021 caagtgtttt gggcggcggc ttcgtatgct tggcggtggc ggcggctgta ccattcggcg
     1081 agttcgcgcc gctcttggag gcggtagctg tcggcggtca ggtagtggtg caggcttgag
     1141 aagccggcgc gttggagggc ggcgcggctg atgtggccga gggcgaggtc tgttgtgagg
     1201 gtgtcttttc cggcggtcag gctgatgttg gtgaagaggt ggcggaatcc gtgcattgtg
     1261 tgtttggatt tgccgggggt gctgccgtca tagcccagtc gtcggatggc gttgtgggcg
     1321 aatttgatgc tgatgtggtc gggatggggg gcgggtttgc gccgtgggcg gatgccgggg
     1381 aaaaggtgga tgttgtcgcc ggtctgtgtg tgcagctctc ggagtatttc taccgcccag
     1441 tccgacagta ggacggtaaa ggggtgtttg gtcttcatgt cggcggcggg gatgtgccat
     1501 aaccgggcgg tgaggtcgat gtcttgccag cgggcggaaa gcagtgcgga cggacgggat
     1561 gacttccccg gtttccccat caatccgcgc cgcctcgatt tttccgttgg cgcgcagcat
     1621 ttccaccgcc tgccgcaccg acatcaaccg cttgccgttg cgcctcatcg cctccaccaa
     1681 aaccgccgaa atcaatttgg cttcttccgg tttaagctcc gtcttgcccg catcgctgcg
     1741 ccgtttgcgc gtcggcttga cgctgaccgc ctccagcttg cggtatagcg tggcaaggct
     1801 gatgcccaat tcctgcgcct gctgcttaag atatgcagag cgtgcgccgc gttccattgc
     1861 ttccgcctga ttctcgactg ccttaagacg ctcaatcatt gccggattca tcgccttctc
     1921 ccgtttcacc gcccaaccat tccggcacat tgtctgtcgg tgcttcagtc ggcagggcat
     1981 agctttcgcg cagttgctcg cagtccaaaa taatttgatt gagcgtgccg accatctttg
     2041 cctgatggct gatcccgtgt gcctcactgt gcgcattaag ttggtcgaac aaatctttca
     2101 gacggctcac ttgactgcgg ataccgacct caaggcttgt taactgcatc gccaactcgc
     2161 tgcctacgtc ttccgccttc ggctctctga caacggtttg cttcttagcc agcttctcgg
     2221 ccagctcatc aattttggct gttttggttt tcatcacttc gtctttggcg gcgaggtttt
     2281 cgcggctttc gcgcagggcg acgcgcagct cgcgcaccgt cattcggtcc acatcgtcaa
     2341 aggtcatgcc gttgacttct tccccttcgg ccaaacccac cagcgtaacg tcttcttcga
     2401 ccagcagctc aagcagcttc gacttgccca aatccatcag cttcggcgcg gctttctgca
     2461 tttgcggggt cgcaaagcgg cgagtggctg acatcagacg tgatgtttct gcgataccca
     2521 gcccaaattg gcttttcaca atttccataa accgtccgtg ttccgtatgc tcttttaaaa
     2581 taatcagcgc acgtcccagt tcgaacatgc cttccatcgt ctgccgtact gcttgacgac
     2641 cgcgttcaac ccaacgctct tcgctataag tctcgccgtt accccactgc tccatcacca
     2701 gcacactgcg catcgctgcc tgattgctta ctttgtcggt tgcgataatt tcaatttctg
     2761 tattcatttt ttatctccaa agtttccgac gtcggaaact ttcaaaatcc gttaatccac
     2821 atcgacccgc ttgccgattt cggcaatctt gctttgcagc cgttcatgct gctgcctgaa
     2881 ccgctctgcg atttgcaggg ttttgatgcc gtaggcgtag ttgccgtttt caagtttgat
     2941 gaccaatccc gaggcaacca aatcatcaat atccctgctg acttgcgatg gcgtcagccc
     3001 cagtccgacc gataaatcct tattgctcag accgataatc ggatgctcgt caagcgcgat
     3061 aaagaccctc aatagccgtt gtaccctttt actttctgcc atccgcatcc tccttatttc
     3121 agtcccagct tcttggcaat ttcgtgcccc ttgccgtaat tgcctttgcg ctgtccgccg
     3181 atcaccagat acacatcgcg cggcttaaag ccgttctctc ttgcccactg cgccagcgtc
     3241 tggccgtttt tggcaaaatt ttctttcaat tcgtctgccg taagcatttc tttggcctgt
     3301 acgcgggaaa atttcagtat ccgtccgtac ggttggttaa tacactcatg attaatcatc
     3361 gcttttctcc ttcatcccca gcaaaaccgc cgcttcgtgg cttttgccaa aattgccttt
     3421 cagcttgccg cgcaagaggt gctccaccgt ggtgcgctcc agattgaaat atttcgccca
     3481 atgcgccttg cacaccccgt tgcgcttaaa ccacccggcc gcgctctcgc gcgtttgcgg
     3541 atagggaata ggcttgaaat tcaaaggttt ttccatatta tttatgcctt tcgtgtgata
     3601 taatgtgttt gtttttaggt ctctttgcac gataaacatc cggtctgttg tgcagcagca
     3661 gggtcttggc ttgttttaaa gtaaacaagc tttttctcgt agctgggtgt atgtgatcca
     3721 tcatgagctg tcgcgcatga gccttcaaag tgttcatttt ttcgtccttt ctcgtgatga
     3781 tttagggtgt ttgtgtttcg atgtggaaat tataggaaga attcttccta ttttgcaagg
     3841 aatatttatg aataactctt ccttttttgg caaccgattg aaagaagaaa gaaaaaaatt
     3901 aaaaatgact caagctgaaa tcgctgaaaa atgtggggtt tcaggaagaa tgtgggggga
     3961 ttatgaacgt ggcatcagcc agccaaaagc ggaacttttc ttccaatttg aaaaggtggg
     4021 tatagacgtt caatacgtca tgcacggcag acgcggcgaa acagcggtca tgccgtctga
     4081 aaccctgaac gccgaagaac aagaactgct ggtcttgttc cgcgaggcgg cagctgccga
     4141 ccgtgaaatg attctgatgg ttgcgcgcag ggcagagaaa aaagcccaaa ctgcgcttgg
     4201 taaagtgagt aatggataaa atggatcaat tcgaattgtg ccaaaaagaa catgtcaatc
     4261 catttgcctt gtcaaagcaa tatctattgg ttgtaacatt cgtcaaatcc agcagtaaaa
     4321 attttcaggc agcattactt tgggcaagaa gtgccaaatt atttgagaat cttgagattg
     4381 gaaaagaaac catctattgc tgcgctttcg ataaaacagc agaacaggct gggatggccg
     4441 gggtattttt gaattatatt gaaaattgga atggcaaaca gatttacatc aatggccgaa
     4501 tccatagtgg cagtatttat gatttgttag gggttttaga ctgctatcaa aaatcacagt
     4561 cctgtcccaa ccctaaaagc cactgttgct ttgtttcaga cgacattttt ctatggcatg
     4621 gatcaagacc aacgtttgaa atcagtcttg atctaactgg aaagaaaaaa gaaacatcct
     4681 ctgcaaagaa atttgtgatg ccttgtatta atttccgtca ccataggatt gaaaaagaaa
     4741 cctacttagg aaattggaat gaacaaattg ccgcattggc agtaaaacaa aatatagatt
     4801 ggtgtccaag ttttgatatt gagaatttta gacagtatga ataattacta tctatatagg
     4861 aattgcagca gcgatgtgtt atgggtcaaa cgtatccaac gccaaatcga cggcagccta
     4921 ctcttgattt ctgacaattc aacctatcca cccatgccct tggcactggc ggaacacccc
     4981 gatattcaaa tcatcgggca ggtagtgcag gtatcaaaag acttgaacta gacacaatca
     5041 aaaagggaaa tagaatgaaa atactcgctt tattaattgc cgctacctgt gctttatctg
     5101 cgtgtggcag ccaatctgaa gaacaaccgg catctgcaca accccaagag caggcacaat
     5161 ccgaattaaa aaccatgccg gtaagctata ccgactatca atcagcagcc aataaagggc
     5221 tgaatgacca aaaaaccggt ctgacccttc ctgaacatgt tgtccctatc gacaatgcgg
     5281 aaggaaagaa tctgctgcat gacttttcag acggcctcac aatcttaacc gttgataccg
     5341 ataaagccga caaaattact gctgtccgag tagtctggaa tacagatgca atgcctcaaa
     5401 aagcggaaaa actgtccaaa gctgccgcag ccttgattgc ggcaaccgct ccggaagacc
     5461 gcacaatgct gcgtgatacc ggcgaccaaa tcgaaatggc gattgacagc cataatgcgc
     5521 aaaaagagcc aacccgagaa tgggcgcgtg gtgggattgc ttataaagtc actgttacca
     5581 atttaccgag cgtggttttg acggcaaaag ctgagtaaat ctattaagta gaaaaaatag
     5641 aaagggaaat gatgattgag aaaagtattt ctattgtaga tggaaaggaa tactccgttt
     5701 ttgctgtatc acacgagttt cgttatacct ttgatgagcc tattttagtc gctgacttga
     5761 ttagttctct aaaagcttat gaaacactga caagtagtta tcttccagca attttgaatc
     5821 agctgtttga tgtcaaaatc caaaaaatca aagtagctgt atctgaaatt gaaagaggat
     5881 ctttccttga aaaactgatt ttcaatttat tcttcaaaga tgaagatgct tataatgaat
     5941 tttgtcttaa aatacgaaaa tttctaggaa cagaaaatca ggacggaagt attaatatgt
     6001 ccaaaatcat tatgtttgca atgactacac ttttaggggt aggtgctggt tatctcttgt
     6061 ttaaaaaccc gccacaagag aagcaggcaa taaccaacaa catcgttacc gtcattaatg
     6121 ctgatagttc tgtcgcactg gatggtgaac atttggtttc agtggtaaaa gaagtaacag
     6181 gaagcagcaa gcaaaaaact gcagaaaatg tggcaaaagt atatgctcca gcaagtaaaa
     6241 ataatggcag tattaccctt gggacagatg atgttcggat tgaacctgtt gcacaacaaa
     6301 ctgtagcaac tttgcctaaa gatgtggact tacgtgatac gccattgact gaagattaca
     6361 ccgatattga tgtgcaaatt cgtgctactg accgtgataa aaattcaggg tggtatgcag
     6421 tcatagacca aattgttcca tcacgtgttc gattagaact gcctgaagat attgatttga
     6481 ataggctggc taacaatgct actatccgtg caaatgtaac agttgagttt gacttaaagc
     6541 aaaatggctc tcgtaagcct aaaaaaatca tcctcacatc tctctctact gattaagttt
     6601 taacccgtat taaaggctta gtcagacggc ctttcctaca atccctgtat tgatttttaa
     6661 ttcaatacag ggatttttcc atgtcagaca agttcaacca attcatcaac cgcgtcctct
     6721 ctcacgaggg tggttacgcc aaccatccca aagaccccgg cggcgaaacc aattggggca
     6781 tcactaagcg caccgcacag gcaaacggct acaacggctc catgcgtgcc atgacgcgtg
     6841 aacaggcaat cagcatttac cgtaaagcgt tttgggagcg ttaccgcgcc gaccaaatgc
     6901 cggaagcggt cgcgttccaa ttttttgatg cctgcgtcaa ccacggttac ggcaatgccg
     6961 cccgtatgct gcaacgcgcc gcaggcgtac cggacgacgg cgttatcgga gcagtcagcc
     7021 tcaaagccat caattccctt cccgaaaacg accttttatt gcggttcaac gccgagcgtc
     7081 tggtctttta taccaagctc ggtacgttca cctctttcgg caagggctgg gtacgccgtg
     7141 tggcgcaaaa cctgattcac gcgtctgcag ataacactga ttaaagggag ataaaccatg
     7201 tcaaaaaagt cactcatcgc cctaatgacc gcagccatgc agcccgattt cagccacagc
     7261 gacctaggca ttcgctacgc catgccgact cagggatgtt ggacgcaagc ccaccgcaag
     7321 agcggggtag ccgccgcgaa acgcgcagcc aaaaaaaagc gtcacaaata accgcctttt
     7381 tccgatggct gggcggcttg gtctctaatc cggccacagg aaaaatcagc cataccaaac
     7441 tatgggcaaa cgtcgccgca gccgccatga cttggaagtt cgtgcaggcg gcggacgcgc
     7501 ccgaatggct ctagtgggct tatggcgcat tggtcggcgg gtatgcatta atcaaacgcg
     7561 gcatcgcggc gattccgcag ttggcagaaa tcaaaaaatc cgcaaatcag gaaggggggc
     7621 ggcaatgatt gaatttgtcc gagccaaaaa acggctgctt tgggcatttg tgcttttgct
     7681 tgtgtggacg tgcggttacc gatacgccgc cgacaaggcc gaagcgaaac aaaccgccct
     7741 gattgccacc tatcggcatt cttctatggt tgcggcggaa caatatgcct tgcagcttaa
     7801 aaaagcgcag gacgaaaggc agcggtggta cgacttttcc caaaaacaag gaagaaagcc
     7861 cgtgaaaaaa cagtatccgc cgcaaacgaa aaaagccggc tatctgaaaa ccaaggaaga
     7921 actgcttgcg gaattggctt gccttaaagc ggaaatggtt gccctaaaaa agcccgatgc
     7981 cttaatccat gggaaagaag tgcggcagaa agaacgcaac tcgtcgcagg gttaaggcaa
     8041 tgccatccgt tgaactgctg ttggagattg tccttctatt accaattggc cgtccaatcg
     8101 gcagaagaca aatatgccga tttgaaacgg catatccatg atatttatcg acgacataag
     8161 ggaagatacg gctaccggag gattgcggca gccatccgtc acgcaggaac accggtcaat
     8221 cacaagaaag tcagccgtct gatggcgaag acggggctga aggcagtgat acggcggcgc
     8281 aaataccgct cgttcaaagg agaagtcggc aaaattgcgc cgaatatcct gcgacgctgt
     8341 ttccatgcag aaaagccgaa tgagaaatgg gtaacggacg ttaccgagtt caatgtaggc
     8401 ggagaaaaga tatacctttc tccgattatg gatttgttta acggggaaat cgtcagttac
     8461 cgtattcaaa tccgcccgac tttcgatttg gccggcgaga tactgaaagg tgcgccggag
     8521 aaaccgggat cgtctgaaaa gccgatactg cattcggatc aaggttggca atatcagatg
     8581 ttttttatca aaagcagttg aaaggcaacg gtctggttca gagtatgtcc cgcaagggaa
     8641 actgcttgga caatgcggca atggaaagtt tcttcggaac gttgaaatcg gaatgtttcc
     8701 atacgtgcaa atatgattcc gttaccgaat cgtaagcggc actgcacgaa tatatccgtt
     8761 actacaacaa cgatagaatc aagttgaaat taaaaggact gagccctgtt cagtacagaa
     8821 ctcagtccct gaaagccgct tgattaaact gtccgacttt ttggggtcag ttcggcttcg
     8881 gcattttttt atccgttttg ggggtaactt gtttggaaag ctgcaagctt ataaataaag
     8941 gattacattt aagttttggg taaccttttt aaaaaaatgc gtgatgactt ttgcattttt
     9001 aaggcgtttt ttggggtaat tcgtgaaaag ttaccccaaa agttacccca taaatggcga
     9061 aaactcaagc atacgccagc atcctgcaac acaaaaaagc cttgaaactg ttgaagttca
     9121 aggctttttt gtgttgcagg atgctgctga aaatagggta tggtggaggc ggggggaatc
     9181 gaacccccgt ccgaaagtcc tctacaaagc gttctacata cttagttgtg tctatttgaa
     9241 aatcttattt ccatcatgcc gaccaacagg ccttttggaa accagttacc ttaagtctta
     9301 tttcctgcca agtaacccgg taggaaacca gtcaatgtaa gatgacgttg cggtggcttt
     9361 cgccacacag cccattgacc gactgctgca acggctagcc ttaagcggct aaagcgtaag
     9421 tttcgtcgtt tgcgactatt tgaattcagt gttttacggg aatctgagac cccggtatgc
     9481 ccgcatctgc ttcgcaaccc ccgtcgaaac caaggtcgcc cccagaaatg gtttgcaaat
     9541 tatacggata ttgtgcggtg ctgccaagtc tgtcggagaa atttgtcagt cttgctgcct
     9601 taatttgcgt ttgagcagga tgcggacgca gccgtcgttg ccttcccggg gttcggcgta
     9661 ggcgagtacg tcggggtgtt gcatcagcca gtttcgggtc atatttttca gaacgggttt
     9721 gtagcctttg gaacctaatc cgctgccgtg gatgatttcg ccgcatacgc cgcgtttttg
     9781 ggtgaatgcg atgaattcgt tgaggacttt ttgggcttct tcctgtgtgt agccgtgcag
     9841 gtcgacatcg gtaacgacgg gatagtatcc gtttttcagg cgttggatgt cgttttttcc
     9901 ctgtccgttt ttgctgaagc tggcgggcgg ctcgttgtat gtgctgccga tgtagaagta
     9961 gttttcttcg tcggcgcggt tgtctttggg acggactttg atgggggttt tgtcggacgg
    10021 tgcataatat tgttgccggt tttttaacgg ggagacttgt ccgactgctt gtgaaaaatc
    10081 gaaatcctgt tcttgttttt gcttgttttt ttcggcctcc tgtttttctg ccgcttcttt
    10141 tttggcctgt ttgcccagtt gtttgaggat gttttggaag tcggtattca tattttttcc
    10201 tgttatttgt ccgatggctg tttcgggcgg ggttttaatt tgccggaatg tttgccaagc
    10261 gggggaggat gattttgttg ccagcgtatg ttttttgaaa gtgtgattgt atatcaaaaa
    10321 gaaatgcggc aaccgtcggc agtgttgatt gccggaaatg cgggccggtc gaaccgatat
    10381 gcccgaacgc ctgataaagt tttaaaaacc tgccttgcga agcaggttaa tggttttgcc
    10441 aatcttgaat tgccggaaac gcgaaacacg gaaatctgat gtt
//
LOCUS       DAXHNE010000059        10448 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.059, whole genome shotgun sequence.
ACCESSION   DAXHNE010000059 DAXHNE010000000
VERSION     DAXHNE010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10448
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.059"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(128..1174)
                     /gene="adhP"
                     /locus_tag="ACFM4Z_001144"
     CDS             complement(128..1174)
                     /gene="adhP"
                     /locus_tag="ACFM4Z_001144"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFC6021186.1"
                     /translation="MKMQAVVVNKNVAGDVEIVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVNADYAVKVPDGLDPAQASSITCAGVTTYKAIKV
                     SSVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(1457..1846)
                     /locus_tag="ACFM4Z_001145"
     CDS             complement(1457..1846)
                     /locus_tag="ACFM4Z_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC6021187.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVR
                     TTLLHNAQTMERYYRQKGTFATYDKNKLKQNEYFDITLSEVSPDHFTLQAAPNPMTND
                     GETCVVTLNDGGTIAASGTNQSCPGFD"
     gene            2072..3250
                     /locus_tag="ACFM4Z_001146"
     CDS             2072..3250
                     /locus_tag="ACFM4Z_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HFC6021188.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKENATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNSDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            3316..5250
                     /locus_tag="ACFM4Z_001147"
     CDS             3316..5250
                     /locus_tag="ACFM4Z_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HFC6021189.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIQEKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(5667..6449)
                     /locus_tag="ACFM4Z_001148"
     CDS             complement(5667..6449)
                     /locus_tag="ACFM4Z_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFC6021190.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            6588..8777
                     /locus_tag="ACFM4Z_001149"
     CDS             6588..8777
                     /locus_tag="ACFM4Z_001149"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFC6021191.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGIV
                     WETDIAPDMDTARILSVQTAFMEEKPLPQSWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGGMTMAALKQVNAQA
                     AKLIEDWAEQGWIETTEAAKPVLRSYHGQASHSEFVLNADQQKASDEIQTALGRFRPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SQMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQSGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPFKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSQFGAAPMLMVRLA
                     ERERAQIFLESVSRQDLHRAVSLWVQVLQQNRDSKIRWSVDVDPQEA"
     gene            complement(8823..9897)
                     /locus_tag="ACFM4Z_001150"
                     /pseudo
     CDS             complement(8823..9897)
                     /locus_tag="ACFM4Z_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222887.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
     gene            complement(9945..>10448)
                     /locus_tag="ACFM4Z_001151"
     CDS             complement(9945..>10448)
                     /locus_tag="ACFM4Z_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6021192.1"
                     /translation="GSVKQLIAHFDRLIDELDKQIDDHTHTHFDGKAQVAEQIKGIGS
                     ITTATLMAMLPELGRLSHKRIASLVGIAPHPRESGETKFKSRCFGGRSAVLKALYMAT
                     VAATRFEPLIRDFYQRLLSEGKPYKVAVTVCMRKLLTISNARMRDYFAENDTAENDTA
                     ENGIRTA"
BASE COUNT         2421 a         2694 c         3034 g         2299 t
ORIGIN      
        1 gacatgttgc gataagggtt ttcagacggt atttcaaaca gaacagatta aaatcaaatc
       61 caaatccata aaaaatgccg tctgaacagc gttcagacgg catttcgatg tgtactgcca
      121 cgtcaaatca gtggtgatgg ccgcagccgc attctttttt catatcgatt accatacggc
      181 cggtgatttt gccttcgcgc atttcttgga aaatggcggg tgcttcatcc aaagcacgca
      241 gttggacttt cggcacaacc aaaccttccg cgccgaattg gaaggcttct tccaaatctt
      301 tgcgcgtgcc gaccaaagag ccgaccactt cgatgccgtc caaaaccaaa cgcgggatgg
      361 acaaatccat cgattccggc ggcagtccga tggcaaccac acgtccgccc gcgcggacgc
      421 aattcacggc agagttgaat gcggcagcag atacggcggt tacgaccgca gcgtgtgcgc
      481 cgcctgtttt ttcctgaatc actttggcag cgtcctcttt ggcggcgttg acaaccaaat
      541 ccgcgccggt ttctttggca aacgccagtt tgtcgtcgtt gatgtcgatg gcgacaacgt
      601 gtgcgccaaa tacttttttc gcgtattgga cccccaagtt gcccaaaccg cccgcgccgt
      661 agatggcaat ccactgtccc ggacgaacac tggaaacttt aatggcttta taagtggtca
      721 cgccggcaca agtgatgctg gacgcttggg cggggtcgag accgtcgggg actttgaccg
      781 cataatccgc attgacgatg cagtgggtcg ccataccgcc gtcggcggtg tagcccgcgt
      841 tcaatacaga gcggcacagg gtttcgcggc cggtattgca gtattcgcaa gagccgcagc
      901 tttggaacag ccaggcgatg ctgacgcggt cgccgacttt cagatttttc acaccgtcgg
      961 caacttcttt aaccaaaccg atgccctcgt gtcccagcac gcggcccggt ttttcgccgt
     1021 agtcgcctgc cgcaacgtgc aggtcggtgt ggcacacgcc gcaatattcg acttcgacca
     1081 acgcctcgcc gtattccaac gggcgaacct cgcgttcgac tatttccaca tcgcccgcta
     1141 catttttatt cacaacaact gcctgcattt tcatgattgc gctccttagt tacggcaaaa
     1201 aaacctgtaa acggaatgtt gtccgatata agggtaagca tacgcttccg catctcacag
     1261 gtcaagtggt atgttgttga aaaatataga ttatatgtta tattataaca tcttggaaag
     1321 gcacggcatc ggggcggttg ccggatgagg ggcggcaggt ttcaagtttg aaaaaccgga
     1381 cggcaaaccc gtaaaaatgc cgtctgaagc tgtgtccgga cggcatcttt acgggtttgc
     1441 gggcttcggc ggaggattag tcgaagccgg ggcaggattg gtttgtaccg gaagcggcaa
     1501 tggtaccgcc gtcgttgagc gtaacgacgc aggtttcgcc gtcgttggtc atcggattgg
     1561 gggctgcctg aagggtgaag tggtcggggc tgacttcgct taaagtgata tcgaaatatt
     1621 cgttttgttt cagtttgttt ttgtcgtagg ttgcaaacgt ccctttttgg cggtagtaac
     1681 gttccatggt ctgcgcgttg tgcagcaggg tcgtcctgac ttccgacagg cggacgcgcc
     1741 ggatgtaggt tttataggaa gggtaggtga taagcgtcag gatgccgagg atggcgacgg
     1801 caatcatcag ctcgagcagc gtaaagcctt tttgaacgtt tttcatagca atgtgtttcc
     1861 atttgtttgt cggtcgacgg cattataacg ccgtatcgga aatggcggaa tatgtaaacg
     1921 gattgaaatt ttcgggaaag cagattgtat aagccattta aaacaaatgg ttatttttat
     1981 tgtcggcagt ttgccgcctt ggatggggca gggacttgcg gtagaatccg cttccgattt
     2041 atgggattga cgcatacaga gaattgaaaa catggcaaaa atgatgaaat gggcggctgt
     2101 tgcggcggtc gcggcggcag cggtttgggg cggatggtct tatctgaagc ccgagccgca
     2161 ggctgcttat attacggaaa cggtcaggcg cggcgacatc agccggacgg tttctgcaac
     2221 aggggagatt tcgccgtcca acctggtatc ggtcggcgcg caggcatcgg ggcagattaa
     2281 gaaactttat gtcaaactcg ggcaacaggt taaaaagggc gatttgattg cggaaatcaa
     2341 ttcgacctcg cagaccaata cgctcaatac ggaaaaatcc aagttggaaa cgtatcaggc
     2401 gaagctggtg tcggcacaga ttgcattggg cagtgcggag aagaaatata agcgtcaggc
     2461 ggcgttatgg aaggaaaacg cgacttccaa agaggatttg gaaagcgcgc aggatgcgtt
     2521 tgccgccgcc aaagccaatg ttgccgagct gaaggcttta atcagacaga gcaaaatttc
     2581 catcaatacc gccgagtcgg aattgggcta cacgcgcatt accgcaacga tggacggcac
     2641 ggtggtggcg attctcgtgg aagaggggca gactgtgaac gcggcgcagt ctacgccgac
     2701 gattgtccaa ttggcgaatc tggatatgat gttgaacaaa atgcagattg ccgagggcga
     2761 tattaccaag gtgaaggcgg ggcaggatat ttcgtttacg attttgtccg aaccggatac
     2821 gccgattaag gcgaagctcg acagcgtcga ccccgggctg accacgatgt cgtcgggcgg
     2881 ctacaacagc agtacggata cggcttccaa tgcggtctac tattatgccc gttcgtttgt
     2941 gccgaattcg gacggcaaac tcgccacggg gatgacgacg cagaatacgg ttgaaatcga
     3001 cggcgtgaaa aatgtgctga ttattccgtc gctgaccgtg aaaaatcgcg gcggcaaggc
     3061 gtttgtgcgc gtgttgggtg cggacggcaa ggcggcggaa cgcgaaatcc ggaccggtat
     3121 gagagacagt atgaataccg aagtaaaaag cgggttgaaa gagggggaca aagtggtcat
     3181 ctccgaaata accgccgccg agcaacagga aagcggcgaa cgcgccctag gcggcccgcc
     3241 gcgccgataa acgaatatgc cgtctgaaca cggaaacggt ttcagacggc atttgttatt
     3301 gatttacgga atattatgag cttgatcgaa tgtaaaaaca tcaaccgcta tttcggcagc
     3361 ggcgagaacc gcgtccatat tttgaaagac atcagcctgt cgatagagaa gggcgatttt
     3421 gtcgccatca tcgggcagtc cggttcgggc aagtccacgc tgatgaacat actcggttgt
     3481 ttggataccg ccggttccgg ttcgtaccga atcgacggca tcgaaactgc caaaatgcag
     3541 cctgacgaac tggcggcatt gcgccgcgaa cgcttcggct ttatcttcca acgctacaac
     3601 ctcttaagct cgctgaccgc aagggataac gtcgcgctgc cagccgtcta tatgggcgcg
     3661 ggcggcaaag agcgttccgc gcgggcggac aaactcttgc aggatttggg tttggcaagc
     3721 aaagagggca acaagcccgg cgaactctcg ggcggacagc agcagcgcgt ctccatcgcc
     3781 cgcgccctga tgaacggcgg agaaatcatc ttcgccgacg agccgaccgg cgcgctcgat
     3841 accgccagcg gcaaaaacgt gatggaaatc atccataagc tgcacgaagc cgggcatacc
     3901 gtcattatgg tcacgcacga ccccggcatc gccgccaatg ccaaccgcgt catcgaaatc
     3961 cgggacggcg aaatcatttc cgacacctcg aaaaatccag aaatccccgc aagcaatgtc
     4021 gggaggattc aggaaaaagc ctcgtggtcg ttttattacg accagtttgt cgaagccttc
     4081 agaatgtcgg tgcaagcagt attggcgcac aaaatgcgtt cgcttctgac gatgctcggc
     4141 atcatcatcg gtatcgcttc ggttgtctcc gtcgtcgcat tgggcaatgg ttcgcagaaa
     4201 aaaatccttg aagacatcag ttcgataggg acgaacacca tcagcatctt cccggggcgc
     4261 ggcttcggcg acaggcgcag cggcaggatt aaaaccctga ccatagacga cgcaaaaatc
     4321 atcgccaaac aaagctacgt tgcttccgcc acgcccatga cttcgagcgg cggcacgctg
     4381 acttaccgca ataccgacct gaccgcttct ttgtacggcg tgggcgaaca atatttcgac
     4441 gtgcgcggac tgaagctgga aacggggcgg ctgtttgacg aaaacgatgt gaaagaagac
     4501 gcgcaggtcg tcgtcatcga ccaaaatgtc aaagacaaac tctttgcgga ctcggatccg
     4561 ttgggtaaaa ccattttgtt caggaaacgc cccttgaccg tcatcggcgt gatgaaaaaa
     4621 gacgaaaacg ctttcggcaa ttccgacgtg ctgatgcttt ggtcgcccta tacgacggtg
     4681 atgcaccaaa tcacaggcga gagccacacc aactccatca ccgtcaaaat caaagacaat
     4741 gccaataccc aggttgccga aaaagggctg accgatctgc tcaaagcgcg gcacggcacg
     4801 gaagatttct tcatgaacaa cagcgacagc atcaggcaga tagtcgaaag caccaccggt
     4861 acgatgaagc tgctgatttc ctccatcgcc ctgatttcat tggtagtcgg cggcatcggc
     4921 gtgatgaaca tcatgctggt gtccgttacc gagcgcacca aagaaatcgg catacggatg
     4981 gcaatcggcg cgcggcgcgg caatatcttg cagcagtttt tgattgaggc ggtgttaatc
     5041 tgcgtcatcg gcggtttggt cggcgtgggt ttgtccgccg ccgtcagcct cgtgttcaat
     5101 cattttgtaa ccgacttccc gatggacatt tcggcggcat ccgttatcgg ggcggtcgcc
     5161 tgttcgaccg gaatcggcat cgcgttcggc tttatgcctg ccaataaagc agccaaactc
     5221 aatccgatag acgcattggc gcaggattga ggttggcaca aagatgccgt ctgaagctgc
     5281 aggaccggtc attttggagc agaaacttat tggacaaaaa cggtttctta gattctacgt
     5341 tctagattcc cacttgcgtg ggaatgacgg cggcgggggt tcgatgattg cacacaacgc
     5401 tcgagtcccg tcattcccgt aaagacggga attcggttcg ttcggctttg cttgtttcgg
     5461 ataaatcacg gtaactcaat attccagatt cccgcccgcg tgggaacggc ggcggggctt
     5521 cgtattgttc aatttattat tttcaatcat tcaatgggtt aggatgtgtt tgttggcttg
     5581 ctaactttca gtgcggattg gttttcagac ggcatttccc gcaaaaaagg cttggaattt
     5641 tccaagcctt ttgttttgcg gatggattat tgatttttgc ggatgatttc ctccagttgg
     5701 ggcatcgggc tgtagccgct ttggctgcgc ccgttgggga agacgagggt cggcgtgccg
     5761 ttgaagccga attgttcgcc caaggaagtg gtttccgcga cgggattgtc gcagatgctg
     5821 ccgccgaccg ggaatttgcc tttacgcatc caatccgtcc acgctttggc gcggtcgggc
     5881 tgacaccata agatttgcgc cttgcgcgcg gcatcggggt gcaggccggc aatgggcatc
     5941 ataaagctgt aaaccgtcac gtcggtcatt ttttcaaact cgtgttccaa gcgtttgcag
     6001 aacggacaat cggggtcgga gaagacggcg actttcagct tgccgttgcc gcgcacttct
     6061 ttgatggctt tgtccaaagg cagggaggcg aagtcgattt tgttcaaatc ggcggcgcgt
     6121 tcttcggtca ggtttttgcg cgtgtcgatg ttgatgagtt cgccgacgaa catatagccg
     6181 ccttcggcat cggtgtagat aatctgcctg ccgctgacga cgacttcgta aatgcctttg
     6241 accggtgttt cgctgacgct caacactttc aaatcttggg cggaataggt tttttccaaa
     6301 cgcgctttca aagaggcggc aacggatttg ccggcggact cggctttgac ggcgggttcg
     6361 gcgttggcat tggaaacggg cgtttgcccg caagccagca gtgggaggac ggtaaagggg
     6421 gtcaagattt tgattaactt ggttttcata taaagatgat tgcgcgtgtt ggaaaagcgg
     6481 aattgtatca aatctctgtt gcgcctgcat tgcgcctagg ctcaatttat cgtctgaaaa
     6541 tagcttccgc ctgttaaaat acgcaaaaaa atgatttgcc tgcctgtatg atctaccacc
     6601 gcatcgccgt aaacgtgccg ctttcagacg gccttttgac ttattcccat tccgatccgc
     6661 ttcctccggg aacgcgggtg cttgtgcctt tccgtaataa aaccgttgtc gggattgtgt
     6721 gggaaacgga tattgcgccc gatatggata cggcgcggat tttgagtgtt cagacggcct
     6781 ttatggaaga aaagccgttg cctcaaagct ggcgtgattt gttggcgttt acgtcgcgtt
     6841 attaccacta tccgactggg caggcggtgt ttgccgcgct gccgcagggt ttgaaggaaa
     6901 cgcgcgcggt ggaaatgccg cagccgccgt tgttttatgc tttgaacgaa gcgggcaggg
     6961 cgcaaacgcc gccgccggcg cggtttaaca aaaaagcggc tttgtgggac gcgctgcttt
     7021 cgggcggaat gacgatggcg gcgttgaagc aggtaaacgc gcaggcggcg aaattgattg
     7081 aagattgggc ggaacagggt tggattgaaa cgacggaggc ggcgaaaccg gtattgaggt
     7141 cgtaccacgg gcaggcttcg cactctgaat ttgtattaaa cgccgaccag cagaaggctt
     7201 ccgatgaaat tcagacggca ttgggacgct tccggccgtt tctgctgtac ggcatcaccg
     7261 gcagcggcaa gaccgaagtg tatttcgatg cgatggcgaa agtgttggcg caggggcggc
     7321 aggtgttgtt tctgttgccc gaaatcaacc tcacgccgca gcttttaaag cgggtggaaa
     7381 accgttttgc tgacgtgccg accgccgtgt tgcacagcca gatggcggca ggcaggcgca
     7441 cgcaggatta tttgcgcgcg atgttggggc aggcgaaatt ggtcatcggc acgcggctgg
     7501 cggtgttcac gccgatggat gatgtcgggc tgattgtggt cgatgaggaa cacgacggct
     7561 cgttcaaaca ggacaacgaa ttacgctacc acgcccgcga tttggcggtg tggcgggcga
     7621 agcagagcgg ctgccccgtt gtgttgggca gtgctacgcc cagtttggag agttggcaca
     7681 aggcgcaaag cggcgcgtac cgcctgctgc aactgaccga acgcgcccat accgccgcgc
     7741 aactgccgca agtggacatc ctcaacgtag gtcgtctgaa actcgacaac ggcttctcgc
     7801 cgcaagcctt gcagcttttg aaacagaact ttgaagcagg cggtatgtcg ctggtgtacc
     7861 tcaaccggcg cggtttcgcg cccgcgctgt tttgcggcga ctgcggccat accttcggct
     7921 gcccgaactg ctccgccaaa atggtgttgc accaacgcgc ccgccaattg cgctgccacc
     7981 actgcgacca ccgcgaaccc attccgttca aatgccccga ctgcggcaac caagacctga
     8041 ccgccgtcgg acacggcacg cagcgcgtcg aagaaaccct gcgcgccttc ctgcctaaag
     8101 ccgccgtcgt ccgcgtcgat agggacagca cggcgcacaa aaatgactgg gcggatttgt
     8161 accgccgtat cgccgacaac gaaatcgaca ttctggtcgg cacgcagatg ctcgccaaag
     8221 gtcatgattt cgcgcggctc aacctcgtta tcgtattgaa cgccgatggc agcctgtata
     8281 gcgcggactt tcgtgcgccg gaaaggctgt ttgccgagct gatgcaagtg tccggcaggg
     8341 cggggcgtgc cgacaaaccc ggcaaggtgt tgatacagac ccaactgccc gaacatcccg
     8401 tcttcgccgc cgtcaaggcg caggattatg ccgtgtttgc cgaaaatgaa ttgaacgagc
     8461 ggcaaatgtt cgcgatgccg cccttcggtt tccagaccgc cgtccgcgcc gatgcgccgc
     8521 gtgttgccga tgcgatggaa tttctcaatg ccgccaaaga aaccctcgcc ccgctgttgc
     8581 ccgaaagcgt ttcccagttt ggcgccgccc ctatgctgat ggtgcgcctt gctgaacgcg
     8641 aacgcgcgca aatcttcctc gaatcggtat cccgacagga tttgcaccgc gccgtgagtt
     8701 tgtgggtgca ggtgttgcag caaaaccgcg acagcaaaat cagatggtcg gtggatgtcg
     8761 atccgcagga ggcttgatta tcggcaatac gatgccgtct gaaaccgttt cagacggcat
     8821 ttttattccg ggtcgcccgt aaacgcattc gcccgaaaaa tcggaacaaa cgtgaaaaga
     8881 taaagcgtga acacgacggc ggtcagaatc gcggggacgg tgatgaagaa tatcgggttc
     8941 acgttcatta aaacagcgcg cgaaacggcg gcggcgaaaa gaatggggat ggcgatgcgg
     9001 cagagtttgg ggtagtcaag tttggtaaag ccgctgtgcc acagtccggc agtcagccac
     9061 accatcatca cgccgcccat catgccgccg agagtaatca ggtgcagggg cgcggaggag
     9121 ggcagatttt gcagtttcgc cgcgcctgtc cacagatagc ctgcggcggc aaagagttgg
     9181 agtaggtaat aggtgcggac gtagtgtttg cgcaggagtt cgtgatggtg cagttcgcgc
     9241 agcttggcga gcaggatgaa accgacggcg aatgcggtaa aaccggcggt ttgcgcgggc
     9301 agccaaagtt cggcggcggc gtgcagcagc aggaaggtga tggcgatgtt tttatagacg
     9361 acgttgggga tgaatacggg gtctttcaga cggcattctt tcaacgcttc cgcacccagc
     9421 aaaacactca cgcgtatgga aacaaacatc accgccgcca tattcaaatg cacttgcgcg
     9481 cgcagtaagt tcaaatcgcc gctgacggca taggccgtct gaaaaacggt aaatgcggca
     9541 agcaacgtca aaagagcgaa gttgtcggtg ttgcggtcga gccaaatcag ccgggcgcag
     9601 aacagcagca ataccagcca ataggcggca acgaaaaacg aggcagtttg cggcgaaaag
     9661 ggcagtatag cggatgcggc gagcaataat gccgccatca aagtcgcgac aggtttcagg
     9721 ttacccgaaa aaaaccgtcc agtccaacaa agccgcagtc aggaaaccac catatgccgc
     9781 aggtagcatg agttctaaga agatttggcg gtgcaggacg acggcaccgg ggctgatgaa
     9841 aaacaccaac gcgccgagta tggcaagcac tgtcgcgccg acgaaaaacg ggcgcatagc
     9901 aactgtattt ttcaccccgt cgggcaaaaa taccaaaact caaatcaagc cgtccggata
     9961 ccgttttcgg cggtatcgtt ttcggcggta tcgttttcgg caaaataatc acgcatccgg
    10021 gcattcgata tcgtcagcag tttacgcata cataccgtaa cggcaacctt atacggctta
    10081 ccctcggaca gcaggcgttg gtagaaatcc cgaataagcg gttcaaaacg tgtcgctgcc
    10141 acggtagcca tatacagtgc cttaagcacc gcagaccttc cgccaaagca gcggcttttg
    10201 aatttggttt ccccgctctc cctcgggtgc ggggcaatgc cgaccaaact cgctatccgt
    10261 ttgtgcgaca gccgccccaa ctcaggcagc atcgccatca gcgtagccgt cgttatcgaa
    10321 ccgatgcctt tgatttgttc tgccacttgg gctttgccgt caaaatgcgt gtgggtgtgg
    10381 tcgtcgattt gtttgtccaa ttcgtcaatc agccggtcaa aatgggcaat cagttgtttg
    10441 acgcttcc
//
LOCUS       DAXHNE010000060        10292 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.060, whole genome shotgun sequence.
ACCESSION   DAXHNE010000060 DAXHNE010000000
VERSION     DAXHNE010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10292
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.060"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(16..771)
                     /locus_tag="ACFM4Z_001152"
     CDS             complement(16..771)
                     /locus_tag="ACFM4Z_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HFC6021193.1"
                     /translation="MKYIRLFFLCTALISTNAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYILHNLGFRCTSR"
     gene            complement(898..2058)
                     /gene="nirK"
                     /locus_tag="ACFM4Z_001153"
     CDS             complement(898..2058)
                     /gene="nirK"
                     /locus_tag="ACFM4Z_001153"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HFC6021194.1"
                     /translation="MKRQALAAMIASLFALAACGGEPAAQAPAASAEAASSAAQAAAE
                     TPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMTMEDGVEYRYWTFDGD
                     VPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTSTFSFK
                     ALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKGKKGA
                     QGLQPFDMDKAIAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLVSSFH
                     VIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRAFNKG
                     ALGQLKVEGAENPEIMTQKLSDTAYAGNGAAPAASAPAASAPAASAPAKSDY"
     gene            2429..4684
                     /locus_tag="ACFM4Z_001154"
     CDS             2429..4684
                     /locus_tag="ACFM4Z_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HFC6021195.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQSTGGMEVGSILGHGAYQAPDWTADWLHRELSTWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRVKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPKLQTTREHFAMKNNTLPSQEAREKLFNFFFWTSWSASTNRPDENFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPELNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGAIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLVFIGGAICVAI
                     QATKIVFGRDK"
     gene            4863..5396
                     /locus_tag="ACFM4Z_001155"
     CDS             4863..5396
                     /locus_tag="ACFM4Z_001155"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFC6021196.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(5459..6643)
                     /locus_tag="ACFM4Z_001156"
     CDS             complement(5459..6643)
                     /locus_tag="ACFM4Z_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFC6021197.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNVGFTVTNQQAGSRRF
                     QRFAGTDAQRVADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNGGYAVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(6683..7087)
                     /locus_tag="ACFM4Z_001157"
     CDS             complement(6683..7087)
                     /locus_tag="ACFM4Z_001157"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFC6021198.1"
                     /translation="MTAQPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEIVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
     gene            complement(7694..8131)
                     /gene="rnhA"
                     /locus_tag="ACFM4Z_001158"
     CDS             complement(7694..8131)
                     /gene="rnhA"
                     /locus_tag="ACFM4Z_001158"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFC6021199.1"
                     /translation="MDQTVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTASKQPVKNDD
                     LWKELDALVGRHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            8245..9114
                     /gene="tehB"
                     /locus_tag="ACFM4Z_001159"
     CDS             8245..9114
                     /gene="tehB"
                     /locus_tag="ACFM4Z_001159"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HFC6021200.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKAENLPEILLSGYSSEGGEWVCL
                     NVLQGDVEVRTQDGAAEVWSAESGDCVFAPQQVFSVKPKTDDAEIRLSMYCTAADYFH
                     KKYGMSATHSAVVAAQDTVPAGRALDMGCGQGRNALFLGLKGFDVTAADHNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGSMHAKDENGNPI
                     RFKFVTMLAKKPE"
     gene            complement(9164..9970)
                     /gene="thiD"
                     /locus_tag="ACFM4Z_001160"
     CDS             complement(9164..9970)
                     /gene="thiD"
                     /locus_tag="ACFM4Z_001160"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFC6021201.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCSFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGSDVCKAVRTAKSYITAAISNPLEIGAGHGPVNHWAYRD"
     gene            10031..10228
                     /locus_tag="ACFM4Z_001161"
     CDS             10031..10228
                     /locus_tag="ACFM4Z_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021202.1"
                     /translation="MPSENRSAGVCGALYAVLQDKAQGRAAGCIVATVFLPDGVKNTV
                     ATPTDNEKREQCRLNRVLRRA"
BASE COUNT         2263 a         2502 c         2908 g         2619 t
ORIGIN      
        1 ctataattga aggggttatc ggcttgtgca acggaagccc aagttgtgca agatatattt
       61 ggattgcagg ctggtacgga tgccgtagcg gaggaaggcg gcatagttgg acgagtcgct
      121 cgacccgata gacgcgccac tgcaaaacat ttgcgcgttg gcattgccgg aagaaagcag
      181 gctgctgttg aaatcttccg tccattccca aatcagaccg tgcatatcat aaacacccca
      241 gtagttcggg cggcctttgc cgacatcgtg caggcctttc cgtccgccgt cggcatacca
      301 atcgagaata gtgcggttgt agccgggttc gtttgagccg tttttctgcg tggcggaagc
      361 aagtccggca aattcccatt cgtcaatggt cggcaggcgt ttgccttgtg cggcgcaata
      421 ggcgttggcg gcaaaccagg aaacattggt taccggttgt tttaattcgc ccgccttcgg
      481 cgcatagctg cggctgccgt ttttcatcca atgcttcagg taagcgggtt ctgcctgttt
      541 ggaaccgatc ctgccttttt gccattgggg gtggctgttg acaaattcgg caaactcggc
      601 attggtaacg ggatatttat ccagtttgaa cggtttgact ttaatcagac cggtatcttt
      661 tttcagataa agcgggcggt agctgccgcc ttcgatttga accatttcgg cagctgccgc
      721 attggtactg atgagtgccg tacagagaaa aaataaccgg atgtacttca tatcatgcct
      781 cctgacaggc agttaaaatc aatcttccga aaggaaagat tggttgttaa aaaaccactg
      841 ccgtgcgtaa tgaagtacgg cggcagtggt tcgtttgcct aaaaatgggt gttcaattta
      901 gtaatcgctt tttgcaggag cagaggctgc aggagcagaa gctgcgggag cggaagcagc
      961 aggagctgca ccgttaccgg cgtaagcggt atcactcaat ttttgagtca tgatttcagg
     1021 gttttctgca ccctctactt tcaattgacc cagtgcgcct ttgttgaatg cgcggaagat
     1081 agagtggtca accaaagtgt agctgcccgg gatgtcgact ttgaattcga cgatggcaga
     1141 gccgccggca ggaacgatgg tgctttgtac gttttcgtta atcagtttgc cgccttccac
     1201 ataaactttg tcgaagattt caccgattac gtggaaggaa gacaccaagt taggaccacc
     1261 gttaccaacg tacatacgta cagtttcgcc tgctttggct ttcagggcgt tgtcgccggc
     1321 gatagcacct acgtgaccgt taaatacaac gtattcaggt tgttcggcga tggctttgtc
     1381 catatcgaac ggttgcaggc cttgcgcgcc ttttttgcct ttggtgtaga agtcgccttg
     1441 gacgatgtag aactctttat ccactttcgg cagaccttct ttaggctcga ccaaaatcag
     1501 accgtacata ccgttggcga tgtgcatacc gaccggcgcg acggcgcagt ggtagatgta
     1561 caggccaggt tgcagggctt tgaagctgaa tgtggaagta cggcccggag cggtaaaggt
     1621 tgcagccgcg ccgccgccct gaccggtagc cgcgtggaag tcgacgttgt gcggaacggt
     1681 agaagaagga ttgttggaga attccacttc aaccgtatcg ccttcgcgta cgcggatcat
     1741 gcggcccgga acatcgccgt caaatgtcca gtagcggtat tccacaccgt cttccatggt
     1801 catggttttt tcgacggttt ccattttcac gcggactttg gcagggtagt cgcggtcgat
     1861 tgcgggaggt acttcaggag cgtgggtggt aactgcatcg atgacgggca gttcgcctgc
     1921 aggcgtttcg gcggcagctt gtgcggcgga acttgcggct tctgcggaag cggcaggggc
     1981 ttgcgcggca ggttcgccgc cgcaggcggc taatgcgaat aaggaagcaa tcattgcagc
     2041 taaggcttgg cgtttcataa tattttcctt ttgtaagaaa agtagaggga ttggttaaga
     2101 acttggtgtg attatgcgtt tgccccattg ggggcatttt gatttaagtc aaataattca
     2161 taaaatatga acttaaaatg atacttctaa tactttatag ctactaaaaa ttacaataaa
     2221 taggctgatt tgcttaaggg cttcggcaaa tcaggcaaag gcagggtagt tgccgactaa
     2281 agaataatga tagttattat catatatgta tttgttttat atgtgagttt ctttgaacaa
     2341 atgtttattt gcgcggtaaa ccgtgctaca atctttaaca ttcatatttt gtgaatttta
     2401 atccactata ttaaaaggag ctctcaaaat gggacagtac aagaagctgt ggtacttgct
     2461 gtttgccgtt ctggcggtat gctttaccat tcttggctat atgggcagcg aggtttataa
     2521 gaaagccccg ccttaccccg aacaggtcgt ttccgcatca ggcaaagtgc tgatgacgaa
     2581 agacgatatt ttggcaggtc agtctgcgtg gcagagtacc ggcggtatgg aagtcggttc
     2641 gattctggga cacggcgcat atcaggctcc ggactggacg gccgactggc tgcatcgtga
     2701 gttgtccact tggttggatt tgaccgcgca acagacttac ggcaaaaaat ttgatgaagt
     2761 ttcccctgaa gaacaagccg ttctgaaaac ccgcctggct gacgaatacc gcaaccaaag
     2821 ccgtgtgaaa gaagacggca gcgtcgtcat cagcgatacg cgcgtaaaag ctatcgaaag
     2881 catcctgcct tactaccacg gtgtttatgg cgacgatccc aagctgcaaa ccactcgtga
     2941 acactttgca atgaaaaaca atacattgcc aagtcaagag gcgcgtgaaa aactgttcaa
     3001 cttcttcttc tggacttctt ggtctgcttc gaccaaccgt cctgacgaga attttactta
     3061 caccaacaac tggccgcacg aacctttgat caacaacgta ccgaccactg aaaactatat
     3121 gtggtcattc accagcgttg ttcttctgct gatgggcatc ggtttgttga tgtggggtta
     3181 ttccttcctg accaaacacg aggaagtgga agtgccgtct gaagacccga tttccaaaat
     3241 ccaactgacc ccttcgcaaa aagcattggg caaatacgtc ttcctgacgg tcgccctgtt
     3301 tgtggtacaa gtattgctgg gcggtctgac cgcgcactac accgtcgaag gtcagggttt
     3361 ctacggcatc gatgaggcgt tgggctttga aatgtccgac tggttcccct atgccctgac
     3421 ccgtacttgg cacatccaat ccgccatctt ctggattgca accggctttt tgacggcggg
     3481 tttgttcctc gcgccgattg tcaacggcgg caaagatccc aagttccaac gtgcaggcgt
     3541 gaacttcctc tatatcgccc tcttcatcgt ggtcggcggt tcttacgcgg gcaacttctt
     3601 cgcgttgacg cacatccttc cccccgaatt gaatttctgg ttcggacacc aaggttacga
     3661 atacctcgat ttgggccgtt tctggcaact ccttttgatg gtcggtctgc tgttatggct
     3721 gttcctgatg ttgcgttgca cggtttccgc cttcaaagaa aaaggcgtgg acaaaaacct
     3781 gttggctatc ttcgtagctt ctatggtcgg cgtgggcgtg ttctacgcgc cgggtctgtt
     3841 ctacggtgaa aaatccccga ttgccgttat ggaatactgg cgttggtggg tggttcacct
     3901 gtgggtagaa ggcttcttcg aagtatttgc cactgccgcc tttgcctttg tcttctacaa
     3961 tatgggcttt gtccgccgca gtaccgccac tgcctccact ttggccgctg ccgccatctt
     4021 tatgttgggc ggcgttccgg gcacgctgca ccacctgtac ttctccggct ctacctccgc
     4081 ctctatggca atcggtgcct gcttctccgc tttggaagtc gtgccgctgg tgttgctggg
     4141 ccgtgaagca tacgaacact ggtcttacca acacctgtcc gactgggcga aacgcctgcg
     4201 ttggccgctg atgtgcttcg tggcagtcgc cttttggaat atgatcggtg ccggcgtatt
     4261 cggcttcctg atcaatccgc cgatttccct gttctatatc caaggtttga acacttctgc
     4321 cgttcacgcg cacgccgcct tgtttggtgt gtacggtttc ttggcattgg gcttcgtatt
     4381 gctggttgcc cgttatttga aaccgaacgc gcagtttgac gacaaactga tgacttgggg
     4441 cttctggctg ctcaacggcg gcttggtcgg tatgattgcc atcagcctgc tgcctgtcgg
     4501 cgcgattcag gcatacgcct ccatcacgca cggtctgtgg tatgcccgca gcgaagagtt
     4561 cctgcaaatg gaaatcctcg ataccttgcg ttgggtacgt acggcagctg acttggtctt
     4621 tatcggcggt gcaatctgcg ttgccatcca agctactaaa atcgtattcg gccgcgacaa
     4681 ataagttttg cggtatgaaa tgagcaactg cacccgtttt cgggtgcagt tttttattgc
     4741 ggattgggac gggaatggaa aaggatgtgg attttttatt ttaatcggct atacaattgc
     4801 tgctttcatg ccgtctgaag cacgtttcag atggcatcgg tttacatcag ataaggataa
     4861 aaatgggtat ttacgatttt caaatgaaag atgcggaagg caatgcggtt gatttgtcag
     4921 gctatcgcgg caaggttctg ctgattgtca acacggcaac gcgttgcggt ctgaccccgc
     4981 aatacgaggc tttgcagaag ctgtatgcac aatataccgc agaaggcttg gagattttgg
     5041 attttccgtg caaccagttc cgcgaacagg ctcccgaaag cagcggagaa attgcccaag
     5101 tgtgtatgat gaagtttggc acgaagttta agattttcga caaaatcgaa gtcaatggag
     5161 caaataccgc gcccttgtat gcctacctga aatccgtcaa accgcaagat aaaggcaatc
     5221 atctgtttaa agatttcgtg ttgaagctcg cagctttggg agagaagcgc gacgaaggcg
     5281 acatcaaatg gaactttacc aaattcctcg tcaaccgcga cggcgaagtg gtcgagcgtt
     5341 ttgcgcctag tgttacgccc gaagaaatcg aagccgatat tcgggcattg ctgtaatctg
     5401 ccgccgtctt gggaaatgcc gtctgaaggc ttcagacggc atttgtccgt ttgcgggttt
     5461 attccgaaat atcggcaaca ccgctttcgg ggaagatagg caaatccggc ggcgggagca
     5521 gggtatccaa agtcagggcg gacgcatcga gtgtggggta gccttcaaac gcgaccgcat
     5581 aaccgccgtt tccattcata cggattcggg cgtgcgcgcc tagagggaaa gtgattttgt
     5641 cggcaatgtg tccgaacggg aagcccgtca gcacggggat tttcgccgtg cgcgaaacat
     5701 ggttggcaac ggcagaaaaa tcataagacg gatcatagac atctcgaatt ttttccatac
     5761 ggaaattgcc gaacacgatg gcgcgctgtt tcttcaaaat acccgaaaga tacagcgtat
     5821 tgagcatacg ttcgatgcgg tagggctgtt cgccgacatc ttcgaggaac aaaatgccgc
     5881 cgtcgatgtc gggcatataa ggcgtgccgg cgagcgaggc gaggacgctt aagttgccgc
     5941 cccacaatat gccttcggtt tcgacatcgg cgcgttggat ataaggaaca tcaacggtca
     6001 ggcggttttg ggttgcaccc ttgataaagg catccatcgt aaacgcaccg ggggcgggtt
     6061 tgccaaaatc gctataagcc atcgggccgg caaaactcat catattgcct tttgccaaca
     6121 atgccagctg gacggcgcat acgtcgctga atccgaaaaa cagcgtgccg tgttcgcgca
     6181 tccttgcgcc gagcgaagca aaatcgatat gcggcagaat ccgcgccgca ccgtaaccgc
     6241 cgcgcaagcc catcagcact ttaggcgtgg cgacgcggcc ggaagcgacc tcttggaaat
     6301 cggcgacacg ttgtgcgtcc gtaccagcaa accgttggaa gcggcggctg cccgcctgtt
     6361 ggttggttac ggtaaaaccg acattgtaaa ggcgggttaa ggctgtgttg acgcggttgg
     6421 tgtcttcggc aaatcctgac gaagcgacaa cgcgcagaag gttgccgtcc gaactttgac
     6481 ggcgtggcgt ttggagaggc acggttcggg ctttcacaac ggaatgggaa gagggaaggg
     6541 gcggaacgga tgtggcggat gtgccgcaag cctgaagcag ccccgcgccg gcagcggcgg
     6601 tgcaggtttt cagaaaacgg cggcgggagg tcggttcggt cataatttcc ctttcatacg
     6661 gtttcagacg gcatcggcgg gcttaaagca gctttttaag ctgttccgtc caatcttgcg
     6721 gcagggtcat accgtgtttc cggacggggt ttgcccaaat cggcgcgggg aaggacgcat
     6781 cgttttcaaa gcgggcgata atatgccagt gcaggtgcgg cacgacattg cccaagctgg
     6841 cgaggttgat ttttgccggc cggaacactt ggcgcatagc ggcttcgact ttgtacacta
     6901 tttccatcaa ttcgccgcgt tccgctgccg aaaggtcggt catttcggca atatgcttac
     6961 gccaaatgac gcggcagaat gcaggcgaac cgctgtcgtt atggacggcg atgacgcgca
     7021 gcttgggggt ttgcagcaaa atatcttcat cttgtgccgt gcagatgggg cagggttggg
     7081 cggtcatttt tccttattcc tgaaagtgtt taaaaaacgg tagggtaatt tttaatattt
     7141 caatctgtct gtccgcattg ccgttaattg taccacttca tagggcggtg ctctatgtgt
     7201 cgtcctgtgt gttgaaacat atcggacgcg tttgaacagt tgtgcggcaa actgttttag
     7261 cggcaggcaa gagatgccgt ctgaagccca ttgtccgtca ttcccacaca ggcagttgct
     7321 acggttgctg aagcccctta gccgtcgcgc aggcggttgc tacggttact aaaatcccgt
     7381 cattcccgcg caggcgggaa tccagtccgt tcggtttcag ttgtttctga taaatcttgc
     7441 agcgttgggt ttttagattc ccgctttcgc gggaatgacg gggcggtcgg atgccgcctg
     7501 aaactccgtc attcttgcaa cttttcgtca ttcccgtgaa aacgggaatc tagaacttca
     7561 aactttcaga taatctttga atattgccgt tgcccgacgg tttggattcc cgcttgcgcg
     7621 ggaatgacgg tggaggacga tgccgtctga agcccattag cggtttcaga cggcattttt
     7681 gccggagcgg cagtcaggaa aactgcgctg cgccacggtt tgccaaatcg tcggcgcgtt
     7741 cgttttcggc gtgtcccgcg tgtcctttca cccaagtcca actgacttga tgccgtccga
     7801 ctagagcgtc gagttctttc cacaagtcgt cgtttttgac gggctgtttg gaggcggttt
     7861 tccagccgtt gcgcttccaa ccgtgtatcc agttttccat gccatttttg acgtattgcg
     7921 agtcggtgca gatgatgacg gtgcagcggc gtttgagcga tttcagtcct tcgatgacgg
     7981 cagtcagttc catgcggttg ttggtggttt gcgcttcgcc gccgaaaagt tctttttcgt
     8041 ggctaccgta gcgcattaac acgccccagc cgcccgcgcc gggattgcct ttgcacgcgc
     8101 cgtcggtgta aaggtaaacg gtttggtcca ttattgtgcc tttgtgtggt gtccagcgtg
     8161 attataagcg gtttgtgcgc cgtgccgtct gaaagattgc ggcggattaa aataggcata
     8221 taatgtgcaa ctttattttt gaatatgaag gagcggatag tgggtcaaag cggcgagttg
     8281 ttttgtttcg gacagatgcc cgtatggaag gcggaaaacc tgccggaaat tttgttgtcg
     8341 ggttattcgt ccgaaggagg ggaatgggtc tgcctgaatg tgttgcaggg cgatgtcgaa
     8401 gtccgcacgc aggacggggc ggcagaggtt tggtcggcgg aaagcggcga ttgcgtgttt
     8461 gcgccgcagc aggtgttttc ggtcaaaccg aaaacggatg atgctgaaat ccgtttgtcg
     8521 atgtattgca cggcggcaga ctattttcac aaaaaatacg ggatgagtgc cacgcattct
     8581 gcggtcgtgg cggcacagga taccgtaccg gcgggcaggg cgttggatat gggctgcgga
     8641 caggggcgca acgcgctgtt cctcggcttg aaagggtttg acgtaaccgc tgccgatcac
     8701 aatccggccg cgttggcaaa cgtggcggag ctggcagagg cggagggttt gaacgtccgc
     8761 acgctggaat atgatttgaa cgccgccgcc ttgcagggcg aatttgatta tattgtggca
     8821 acagtggtgc tgatgttcct gatgccgcag cgcgtgcctg acgtgattgc cgatatgcag
     8881 gcgcatacgg cggcgggagg atacaatttg atcgtatcgg caatggatac ggcggatttc
     8941 ccctgtccga tgccgttccc ttttaaattt aaagagggcg agctgaagga ttattatcgg
     9001 gattgggaac tggtcgaata taaagaagaa ttgggttcta tgcatgccaa agacgaaaac
     9061 ggcaatccga tacggtttaa atttgtaacg atgctggcga aaaagcctga ataatcctca
     9121 aaaatgccgt ctgaacattt tgtttcagac ggcattttta cgtctagtcc cgatacgccc
     9181 aatgattgac cgggccgtgt cccgcgccga tttccaaagg gtttgagatt gccgccgtaa
     9241 tgtaggactt ggcagtccgt acggctttgc aaacgtccga gccttttgcc aactcggcgg
     9301 tgatgcaggc ggaaaacgtg cagcccgtgc cgtgcgtgtg ggcggttgga aagcgcgggc
     9361 tgtccaattc cagcgtttca ttttgcgtaa acagccaatc cgtgcagcgt ccgcttgtgc
     9421 tgccgttcaa atgtccgcct ttgataacga catttttgac accgtaatca agcaggattt
     9481 ttgccgcacg ttccgcatct ttacggtttt cgatatgcac gccggtcagg gcttcagcct
     9541 cgggcaggtt gggggtcaat acatccgtat cgggaagcag caggcgcgtc agtgccgcaa
     9601 cggcggaatc ctgcaacagc ggcgcaccgc ctttggcaat catcacaggg tcgagtacgc
     9661 gcctgccaaa gctgcagtgt ttcagcttgt cggcaacgca ttcgatgatt tccgccgtgc
     9721 cgagcatacc gattttgtag gcgcggatgt cgaagtcttc cctgattgct tggatttgtg
     9781 cggtgatggt ttcggtcggg acgagatgaa ccgccgacac gcccaaggta ttttgcgcgg
     9841 taacggcggt gatgacgcac gttccgaaca cgccgcgcat ctgaaatgtt ttcaaatccg
     9901 cctgaatgcc cgcaccgccg cccgaatccg aaccggcgat ggtaagcgtt tgcacaaaag
     9961 agcttttcat ttgttcctcc ttaatattgc gtccgataaa aagggctaat gcctgtaaaa
    10021 aagcacggcg atgccgtctg aaaaccgcag cgcgggggtt tgtggtgcat tatatgccgt
    10081 attgcaggac aaggcacagg gcagggcggc ggggtgcatc gtagcaactg tatttttgcc
    10141 cgacggggtg aaaaatacag ttgctacacc cactgataat gaaaaaagag agcaatgccg
    10201 tctgaaccgc gtgttgcggc gagcataaac cggcacgctt tattcgtcat ttccacaaaa
    10261 acagaaacct aaaattccgt cattcctacg aa
//
LOCUS       DAXHNE010000061        10153 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.061, whole genome shotgun sequence.
ACCESSION   DAXHNE010000061 DAXHNE010000000
VERSION     DAXHNE010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10153
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.061"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(106..498)
                     /gene="rpsI"
                     /locus_tag="ACFM4Z_001162"
     CDS             complement(106..498)
                     /gene="rpsI"
                     /locus_tag="ACFM4Z_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFC6021203.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(508..939)
                     /gene="rplM"
                     /locus_tag="ACFM4Z_001163"
     CDS             complement(508..939)
                     /gene="rplM"
                     /locus_tag="ACFM4Z_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFC6021204.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(1117..1296)
                     /gene="ssrS"
                     /locus_tag="ACFM4Z_001164"
     ncRNA           complement(1117..1296)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACFM4Z_001164"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1302..1607)
                     /locus_tag="ACFM4Z_001165"
     CDS             complement(1302..1607)
                     /locus_tag="ACFM4Z_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFC6021205.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(1618..1947)
                     /locus_tag="ACFM4Z_001166"
     CDS             complement(1618..1947)
                     /locus_tag="ACFM4Z_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021206.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QRQETQQ"
     gene            complement(2002..2991)
                     /locus_tag="ACFM4Z_001167"
     CDS             complement(2002..2991)
                     /locus_tag="ACFM4Z_001167"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFC6021207.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(3114..5816)
                     /gene="ppc"
                     /locus_tag="ACFM4Z_001168"
     CDS             complement(3114..5816)
                     /gene="ppc"
                     /locus_tag="ACFM4Z_001168"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFC6021208.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPDVRVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRSELDKLYRELPLSIRRVKVNGDVMALSDKSPDEEIARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAVLLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPRSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAAVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            5859..6752
                     /locus_tag="ACFM4Z_001169"
     CDS             5859..6752
                     /locus_tag="ACFM4Z_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC6021209.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDELLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGIGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAEALAGRLKRINHTVNVHTVNEKLDGCRL
                     TDLVQAADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPICGTER"
     gene            complement(7162..7386)
                     /locus_tag="ACFM4Z_001170"
     CDS             complement(7162..7386)
                     /locus_tag="ACFM4Z_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HFC6021210.1"
                     /translation="MDAVQELEHRITELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDTQEPYSLRDEIPPHY"
     gene            complement(7387..8775)
                     /locus_tag="ACFM4Z_001171"
     CDS             complement(7387..8775)
                     /locus_tag="ACFM4Z_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HFC6021211.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLTGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(8861..9682)
                     /gene="prmC"
                     /locus_tag="ACFM4Z_001172"
     CDS             complement(8861..9682)
                     /gene="prmC"
                     /locus_tag="ACFM4Z_001172"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC6021212.1"
                     /translation="MTFDKWLGLSKLPKNEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISPPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(9672..10133)
                     /locus_tag="ACFM4Z_001173"
     CDS             complement(9672..10133)
                     /locus_tag="ACFM4Z_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021213.1"
                     /translation="MQEQNRKSSFPIVMLLVSVALWIASLSNVAFYLGNHGSMEGLTV
                     LILGSIFASLDIRYCAVYANYVWLAAIVLLALRKKVVPVHAAFWGLALVAFSVKAVYV
                     DEAGNTSDIVRYGAGFYLWYAAFAVASIGTFAGKNKERKAASAADGTKNDV"
BASE COUNT         2163 a         2778 c         2796 g         2416 t
ORIGIN      
        1 tgtttttgat ttttgcccaa tctgtaatct ttagattgcc aatgggaaac cgtctactac
       61 aaataaaaaa ccctgcgata agcagggttt tttgaatttc caacattaac gtttggagaa
      121 ttgttttgca cggcgtgctt tgcgcagacc cggtttttta cgttcgactt cgcgggcatc
      181 gcgagtaacg aaaccggctt gagacaaggc gggtttcaac gcggcatcga agtcgatcag
      241 ggcacgggta atgccgtggc ggattgcgcc ggactggccg gtttcgccgc cgccaacaac
      301 attgactttg atgtcgaaag attcggcgtt ttcagtcaga accaagggtt ggcgaacaac
      361 cattcggctg gtttcgcgtg cgaagaattc gtcaacggga cggccgttta cgatgatttg
      421 acctgtacct ttaatcagga atacacgagc cactgaactt ttgcggcggc ctgtgccgta
      481 gtagtattta ccgttcatgt cgcgtcctta tttcagttcc aaaactttgg gttgttgcgc
      541 agcatgggcg tgttccgcac ccgcatacac tttcagtttt ttaatcatgg cgtaacccaa
      601 aggacctttg ggcagcatac cttttacagc ttgttccaaa gcgcggcccg ggaattgctc
      661 ttgcatttcg cggaaagtgc gttcgtagat accgcctggg aaaccggaat ggcggaagta
      721 ttttttatct tcgaatttgg caccggttac acgcagtttg tccgcattga taacaatgat
      781 gtaatcgccg gtatcgacgt ggggggtgta ttcaggtttg tgtttgccac gcagacggct
      841 ggcgacttcg gccgcaacgc gacccaagac tttgtcttgg gcatcgatga cgaaccattc
      901 gcgcttcacc tcgtggggtt tcgctgaaaa ggttttcata gtggaaatcc agatagatat
      961 agaaagttgt aaattttaaa gacaggattc gattttgtca atcgcattac cgcgttacgg
     1021 aaggattttc cggatttcgg cagactgcat actgcttttt cgggcggggc ggcggccaat
     1081 gtgaaaaacc gcatcgttgc gatgcggttt tgaatgggaa tccccgcgag agccgtttcg
     1141 gccgaatccg cttgaacctt gctgacaagg cggctgcctc gggtagtttc gggtgcgtcc
     1201 gcaaaaggac gctcgcgccc actactgctc ccggcaacct taagcgaact tattggttca
     1261 aaggaatata tgccttcgcg gacaccgcag ggaaaaaggg gttattcctg cgccaagcgg
     1321 gatagtgctt tttggcaggc gttgtccata tcggctattt tacgcgcaaa atcgccgatt
     1381 gccaaatcgc cgccgttcag ggaggttttc aacaggtcgt ggacgacgtt gagcgcggcc
     1441 ataatgacga ttttttcgct gtccgcgacg cgtccgcctt cgcggatggc ttcggctttg
     1501 ccgttgagca ttccgactgc ctgcaacagt gtgtcttttt cttctgccgg cgtgttgacg
     1561 gtcagccggg cgtgcatgac ttcgatgtgg acttgttcga tgttcatcct ttaatcctta
     1621 ttgctgcgtt tcctgccgtt gggggaggcg cgctgccagt gcgctgattt tttccctgct
     1681 ctgttcgagc aggctgcggt atcgtgtatt ttcttctgtc aggctgtcaa ttttgttttg
     1741 caggtcttct ttgagtttgc cgacttggac gagcagggct tcgctgagtt cgtcgacggc
     1801 ggtttcgtgt tcgagttttt gccgctcgtg cgcccgtttg agttcggcga cggtttcttt
     1861 gaggcggcgg ttttcgctga cgagggtttc gaatttttgt accagcgtat aaacgctgct
     1921 ttcgagtttt tcgatatttt gtttcataac cttacctgtc cgtatgccgt ctgaaggctt
     1981 cagacggcat ctgtctgttg tttattcgaa acgcgcgctg cgttccatca gtctttcgac
     2041 gacctgctga ggggtcattt ctttgcggat gagttgcagc agggtttggg taatcggcat
     2101 gtcgatttgg tacttacagg cagtattgaa gacttcttct atcgtgctga ccccttcgga
     2161 aacgtgtccg atttcgacca gcacctgatg cagttccttg ccttctgcca aacccaagcc
     2221 gacgcggcgg ttgcgtgaga gtgcgccggt gcaggtcaag atgaggtcgc cgatgccagc
     2281 aagccccatc atggttttgg gctgtgcgcc cattgcggag gcaaggcggg tgatttcagc
     2341 taatccgcgt gtaaccagtg cggcacgggc gttaagcccg tactccagac cgtcggacaa
     2401 tccggtggca atcgccatga cgtttttgac tgcaccgccg actgccacgc cgataacgtc
     2461 ggtactgccg taaagcctca tgacggtcgt gttgagctgc ggtacgagtt cttcaatcca
     2521 ctcttggttt tcggaggcga ggacgacggc gcagggcagt tgtttggcga gttcctgtgc
     2581 aaaactcgga ccggaaagta cgccgatttt tttattgtcg ggcaatactt ctttcaatac
     2641 ttgaaaggtc agcagccctg tatcctgctc gaatcctttg caggcggcga ggacggggag
     2701 gtgtcccgcg ccgtactgtt tgagcagctc tgcgctgctt ctcaatccgg caacggaggt
     2761 tacgataagg acaagtccgc tgtctttgag cgcgtctgcc aaatccgcac acacttccaa
     2821 ggtttcggga aaggaaaagc cgggcagtcc gcgtttgttt tcacgcgctt cctgcatttg
     2881 gcggacttgg tctgcgttgc gcgtccacag ggatacgcgg ttgccgtgtt gggaaaaatg
     2941 cagggcgagc gccgtacccc acgaacctgc gccgataacg gtaattttca ttggtcgtct
     3001 ttcaacatat cactgccgtt cactttaaaa caatcggtgt ttctctgcaa gtgcggtcag
     3061 ggaaatgccg tctgaaaggc gttcagacgg cattttgccc cgatgcggca ctatcagcct
     3121 gtattgcgca aaccttgcgc cacgccgttg atggtcaggt gaaccatcag cagcgcgtgc
     3181 ggattgtcgg gttctttacg caggcgtttg agcatggcga cctgcaggcc gttgagcgcg
     3241 ttcaaatatg gaatcctcaa ggcaagcgaa cgggcgaggc tgcggttgtc gcgtaaaagt
     3301 tcttcggttt gcagcaggtc gagcagggct ttgcggctgc gttggtattc ttccttaatc
     3361 atgccgaaga tgacggcggc tttttccggc gattcgctca agccggcata gttttccgcg
     3421 agggtgatgt cggttttcgc catcacttgc tccatattgg aaagcatggc ttggaagaac
     3481 gggttgcttt gggcgtgttc gcgcagggcg gcgagcgttt cgggtttgtc ttcgcacaag
     3541 gtttccaccg cgctgccgaa accgtaccaa gccggcagca tgaggcggtt ttgcatccag
     3601 gaaaataccc acggaatcgc gcgcaagtcc tgaatccgcg ccaaggtttt gcggctggcg
     3661 ggacggctgc ctaggttgag ggtggcgatt tcctgaatcg ggctggtttg cagaaagtag
     3721 tcgatgaagt cgggatgggt aatcagttcg cggtagtatt tgaacgatac gtccgacaat
     3781 gcctgcatca gtttggcatc agggtctttt ttatccggca ggatgctggc ttccaaagtc
     3841 gcggcaacca gggtttccaa gttgcgttgg gcattgccgg ggtcggcgta tttggcggta
     3901 atgacttcgc cttgttcggt gatgcggatt tgtcccgcca cgctgcccgc cggttgggcg
     3961 agaatggctt ggtaagaagg gccgccgccg cgtccgacgc tgccgccgcg gccgtggaac
     4021 aggcgcatgc ggacatcgta ttttttgaag agttcgacca agcccaattc cgcctgatac
     4081 agacaccatg agctggtaac gtagccgccg tctttattgg agtcggagta gccgagcatg
     4141 atttcttgga tattgccgcg gctttcgagc agggcatcgt accagtcgag gcggaacatg
     4201 gtttccataa ccggacaggc gttttccaac gcttcaatgg tttcaaacag cggcacgata
     4261 ttgatgcggc tgcgcggctt gccgttttcc accgccaaca ggccgctttc tttcagcagc
     4321 aatgccaagg caagcaggtc gctgggttgt tcgcagttgg aaataatgct ttgtgtaacg
     4381 gcatcttcgc cgaattcgtc tttgattttg cgcgcttcgt taaaaatcgc cagttcgtgg
     4441 cgggtatggt cgctgtatgt gatgaacggg ctgtacagcg gacgttgatg gctcaattcg
     4501 cgcaacagga cggcttgttt ttgctcttcg ttcaggctgt tgtagtcttc caagcctgcg
     4561 tgttggaaaa gctcggcaac cacatcggcg tgtttgcctg cgtgttggcg caggtcgagc
     4621 ggcatcatgt ggaagccgaa cacggacacg gaacggatga ggtctgccaa acggccttcg
     4681 gcaagcaggc ggctgccgtt gtcgataagg gaacgttgca attttttcaa atcatcgaga
     4741 aattcttgtg ccgaagcata aggctcgagg aagccgaatt tgcaacccat acccaaaccg
     4801 agcgcgcgcg ctttgcccat ggcgcgcgcc atgatgtagg cgatggcgcg gcggtagggt
     4861 tcttccgtgc gggcgatttc ttcgtcgggc gatttgtcgg acaacgccat cacatcgccg
     4921 ttgactttga cgcggcggat ggagagcggc agttcgcggt aaagtttgtc gagttcgctg
     4981 cggtaaaaac ggaacacggc atcggcatgc cgtctgaacg caaagcgcag ggtttcggca
     5041 gaaacgaacg gattgccgtc gcggtcgcca ccgatccagc cgccgatttt gaggatgttc
     5101 ggaacgcgga catcgggata ggccgtctga aagtcgtgtt ccatcttgcg gtagagtttg
     5161 ggcagggctt caaaaaagct catcgggaag atggacacgc cgttgttgat ttcgtcgttg
     5221 acgctgagtt tgtggcggcg cgtttcgctg gtctgccaca agcccagcag gatagtgtcg
     5281 atttcgcggc gcagccgtgc cagcgcgtcg gcattggtgc agcgttcgcg ttgcggcaac
     5341 agtgcgcgga tgcggcggtt gaagcttaag acggtttggc gttgcacttc ggtcgggtgc
     5401 gcggtcaaaa cggcggtaac ggacgtattg tccaactgcc gctgcaccga tttgccgtcg
     5461 gctttccccg ctttgagcct gcggacggtt tccgtcaggc tgccttccgc gccgccgcgt
     5521 ccggcttctt cgtggatttg gcggcggcgt tcgtggtgca cgtcttcggc gatgttcaaa
     5581 atctgggcga acaggccgca ggccaaggtt aaatcgtggg tttgttgttc gtccaattgc
     5641 ggcaatactt tttcaatcaa tgccgcgctg tcgtcggaag tggacaagag tttgactgtt
     5701 tcgacaacca acggcgaggc ttcttcgtgc aggaggttga acagggattg tttcagaaat
     5761 tccgcgtccg ccgccaaagc cgcgtccttc ggattgttca gaatatgcag ttgcatgatt
     5821 tttctctcat tgccgtaaat actgtaaatg tacctcaaat gccgcatccg tgccaaaccg
     5881 ttcacacttt aaccgcccgt gtcccgaaat gccgtctgaa gttgaacgcc gcccgacggc
     5941 agcgttacaa tcgcccgcaa ctgttttttc cgaacatcat catgaccacg accgaacacg
     6001 acaacgacga cgaactcctg ctgcggtaca gccgccacat cctcttggac gaaatcggca
     6061 tcgaagggca gcagaaactt tccgccgcgc atattttggt cgtcggctgc ggcggtttgg
     6121 gtgccgccgc cctgccctat ctcgccgctt ccggcatcgg cacgctgacc atagccgatt
     6181 ccgacacggt cgaactgcac aacctgcaac gccaagtcgc atttgacgag ggcgatgtcg
     6241 gcaagcccaa agccgaagcc ttggcaggcc gcctgaaacg catcaaccat accgtcaacg
     6301 tccacaccgt caacgaaaaa ctcgacggct gccgcctgac cgatttggtt caagccgccg
     6361 acatcgtttt agactgctgc gacaactacg ccacgcggca agccgtcaac cgtgcctgcg
     6421 tgcaaacgaa aacaccgctg gtttcaggag cagcggtgcg ctttgaaggg caacttgccg
     6481 tgtaccgtcc cgacttgccc gactcgccgt gttacgcctg cctgtttgac ggcggatcgg
     6541 cttcagacgg catctgttcc ctcttcggcg tattctcgcc gctggtcggc atcatcggca
     6601 gtacccaagc ggcggaggct ctgaaaatcc tgctggatgc gggcgaaccg tcgcacggca
     6661 ggctggcggt ttaccgtgcc ttggaagggg gctggcaata tttcgacctg ccgcgcaacc
     6721 ccgaatgccc gatttgcggc acggaacggt aaaaccctgc cgccgtttca gacggcataa
     6781 agacggatgc ggcggaacgg ttttaaaaat ttaaaaattt acatttcttt gcaaaaaaaa
     6841 aaaaaaaaaa ataaacttac cttataattg cagttgtctt agcaatgtct gtttcgcaga
     6901 ctcattgagt aagacgtttt ccccgtaatg tgtttggccg tctgtcccct ttgggttcgg
     6961 acggcttttt tttggctgtg tatgaatacc cggttggttt tatctgttta caggggaaaa
     7021 ccgcttattt ccgttcgggc ggaaaacggt tccatcggat aaaaggcatt ttgtccgact
     7081 gattaaagtt acatcttagg tttgcatcgg caaaatattc aaacacagcc ttttttaagg
     7141 aaatccggat acggcggcac atcaataatg cggcggaatc tcgtcgcgca gggaatacgg
     7201 ctcttgcgtg tcgggattcc tgtcctgcat tttttgatac agcagcctca actgagcctg
     7261 ctgcaaatcc agcgtctgcc gcaattccgc caccatcgcg ttcaggccgg cgattacgtc
     7321 ctcctgaagc gcggattgga tttccagctc tgtgatgcgg tgttccaact cttgaaccgc
     7381 gtccatttac agcaccatcg cggcaatcca gccggcaatc agcagcggga tattgtagtg
     7441 gatgaaggtc gggataacgg aatcgcggat gtggtcgtgc tgcccgtcgg cgttcagccc
     7501 catcgtcggg cccagcgtgg aatcggacgc aggcgaaccg gcatcgccca acgcccccgc
     7561 cgtgccgaca atggcgacgg tggcaagcgg cgaaaaaccc aaaccgacac acaaaggcac
     7621 ataaatcgcg gcaataatcg gcaaagtgga aaaggacgaa ccgatgccca tcgttaccaa
     7681 aagccccacc accagcatcg ccaatgccgc catacctttg ctgttgccga atatcgccat
     7741 actgctttcc accagcggct ggatatgccc ggtcgcattc atcacggcgg caaaaccctg
     7801 cgcggcaatc ataatgaagc cgaccatcgc catcatcttg ataccttcgc cgaatacgtc
     7861 gtttgccttg tcgcggttga tgacccccaa catcataaat acggcgaaac cgagcatcgc
     7921 gcccaacacc agcgagtctt catacatcaa ctggatggca aaacatacgg caatggcggc
     7981 ggcggcggcc aggctgcggt aggcggacgg ctgcggacgg tttgccgcat cggcgttgcc
     8041 cgccgtatcg gcattgttgc tttggtacag gcgcggtttg cggtaatgga caaacgccag
     8101 caggagtccg gtcagcattc ccaacgcggg aatcgccatt gccgccatta cgttgatgtt
     8161 tttcacatca agctgcggcg cggcggaatg gatgttgccc aacaggattt cgttcaaaaa
     8221 aatcgcgccg aagccgtaag gcaggaacat ataggtcgta accagcccga aagtaatgac
     8281 gcacgcaatc agacggcggt cgattttcag gcggttgaac accaaaagca gcggcggaac
     8341 aatcatcggg ataaaggcga tgtggatagg tatgatgttc tgactcataa tgcccatcac
     8401 aaggatgatg gaaagcagca gccatttgac cgcgccctcg cccgaacgca cgctgtcggg
     8461 cataccgccc cggttcagct tgcggacgac cgcgccggca agctgctgcg gcaggccgga
     8521 atgggtaatc gccattgcaa acgcgccgag catcgcataa gaaagcgcaa tcttcgcacc
     8581 gccttccaaa cctttgttga acacggggat aatccccgcc tgactgacct gtcccgccgc
     8641 atcggcaatg ttttgcagcg gcatacccgc caccgcgccg ccgacaaacg cgccgaccgt
     8701 caggctcaat accacgtgca cgcgcgacag cgacagcacc agcataacga ttacggcaac
     8761 tacgactgca ttcattgttt atcgctccaa acctataaat gtttacatat cgaaacacat
     8821 cataacccaa taatggaaaa cccgccaatt ttgcaaacaa ttatttcaaa tgcttcatat
     8881 acttccccag cgtaaccctg tccaaacccg ccaagtccgg cagggtttcc actcctgaaa
     8941 aaccattctc cgccaacacg ccgcgcaccg ccgcgccctg atcgaaaccg tgttccaaca
     9001 acaaaaaacc gccttccgcc aaacggtcgg gcgcgccttg cgccaaggtg cggatgcagc
     9061 ttaggccgtc tgaaaagtcg gtcagcgcga tttgcggctc aaaccgcaaa tcgccttgca
     9121 acaaatgttt atcgccgttt tcgatatagg gcgggttgga cacgatgatg tcccatttcc
     9181 cttcagacgg catatcggtg tcgaaccacg aaccgtgtgc aaattcgacc cgcgcgccca
     9241 aatccgccgc atttttccgc gccgtttcaa gggcgggcgg gctgatgtcg gatgcgcgca
     9301 caaacgcatc ggggcgttcg agcgcgacgg ttacggcaac cgcgccgctg cccgtcccca
     9361 aatcccacac gcgcccgttt tcgggcaggc gcgccaatac ggcttcgacc aaatgttcgg
     9421 tttcggggcg cggaatcagc acgctcggat tgactgtaaa gcgtctgcca taaaattcgc
     9481 gcacacctaa aatataggca accggctcgc cgttcagacg gcgttgcgcc agcctgtccg
     9541 cccgctgtcg gacttcgtcc ggcatttctt ccccgccccg cgtcaacaac tgcacgcgcg
     9601 tatattccga aacatattgc agcagcattc ttgcttcatt tttaggcagt tttgacaagc
     9661 ccaaccattt atcaaacgtc atttttcgtc ccgtctgccg ccgatgcggc ttttctttcc
     9721 ttattctttc cagcaaacgt accgatggag gcaaccgcaa atgcggcata ccacaaataa
     9781 aatcctgcac cgtagcgcac aatatccgat gtattccctg cttcatcgac gtatacggct
     9841 ttcacactga aagccaccaa cgccaagccc caaaatgccg catggacagg cacgaccttc
     9901 ttccgcaacg ccagcaaaac aatggccgcc aaccaaacat aattcgcata gaccgcacaa
     9961 tacctgatat ccaaagaagc aaatatcgac cccaaaatca aaacggtcaa accctccatg
    10021 cttccatgat tgcccaaata aaatgcaaca ttggataaag acgctatcca cagggcaacc
    10081 gacaccagca acatcactat gggaaaactt gatttccgat tctgttcctg catggtttta
    10141 tcctaatgta aaa
//
LOCUS       DAXHNE010000062        10002 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.062, whole genome shotgun sequence.
ACCESSION   DAXHNE010000062 DAXHNE010000000
VERSION     DAXHNE010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10002
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.062"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..635
                     /locus_tag="ACFM4Z_001174"
     CDS             <1..635
                     /locus_tag="ACFM4Z_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6021214.1"
                     /translation="KPTGTDKDKISTVSDYFRNIRAHSIHPRVSVGYDFGDWRIAADY
                     ASYRKWNNNKYSVNTKKVQENQGTRKDLKTENQENGSFHAVSSLGLSAIYDFKLNDKF
                     KPYIGARVAYGHVKHQVHSVEKETEIVTTVPTGGAQTGGTILQGPTPKPPYHESNSIS
                     SLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYRF"
     gene            complement(609..818)
                     /locus_tag="ACFM4Z_001175"
     CDS             complement(609..818)
                     /locus_tag="ACFM4Z_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021215.1"
                     /translation="MPSERLSDGIFPCVLNRIHETPDAFGGNVGCRAMFGGDQRTKYA
                     GKGLMPSEGSDGIGIGESEAVAHAQ"
     gene            complement(836..1393)
                     /locus_tag="ACFM4Z_001176"
     CDS             complement(836..1393)
                     /locus_tag="ACFM4Z_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC6021216.1"
                     /translation="MDALKLLTNRRSSKKLKPPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFIVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMSPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(1469..3103)
                     /locus_tag="ACFM4Z_001177"
     CDS             complement(1469..3103)
                     /locus_tag="ACFM4Z_001177"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFC6021217.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGDIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(3173..3510)
                     /locus_tag="ACFM4Z_001178"
                     /pseudo
     CDS             complement(3173..3510)
                     /locus_tag="ACFM4Z_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3510..4616)
                     /gene="serC"
                     /locus_tag="ACFM4Z_001179"
     CDS             complement(3510..4616)
                     /gene="serC"
                     /locus_tag="ACFM4Z_001179"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HFC6021218.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHINRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTRDEELDRRFVLEAELQGLCLLKGY
                     KSVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4981..5412
                     /gene="rimP"
                     /locus_tag="ACFM4Z_001180"
     CDS             4981..5412
                     /gene="rimP"
                     /locus_tag="ACFM4Z_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFC6021219.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKESGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTISFDGKTVQIELGNIDKARLRPEFKF"
     gene            5440..6942
                     /gene="nusA"
                     /locus_tag="ACFM4Z_001181"
     CDS             5440..6942
                     /gene="nusA"
                     /locus_tag="ACFM4Z_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFC6021220.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDYLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6954..9842
                     /gene="infB"
                     /locus_tag="ACFM4Z_001182"
     CDS             6954..9842
                     /gene="infB"
                     /locus_tag="ACFM4Z_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFC6021221.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAATRAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAGKAQADKMPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFIDVSAKKGLNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMLKGYNEIMEGDQLECFDIVEVARSL"
BASE COUNT         2694 a         2873 c         2517 g         1918 t
ORIGIN      
        1 cgaaaccaac cggtacagac aaagacaaaa taagcacagt aagcgattat ttcagaaaca
       61 tccgtgcgca ttccatccac ccccgggtgt cggtcggcta cgatttcggc gactggagga
      121 tagcggcaga ttatgccagt tacagaaaat ggaacaacaa taaatattcc gtcaacacaa
      181 aaaaggtgca agaaaaccaa ggcaccagaa aagacctgaa gacggaaaat caggaaaacg
      241 gcagcttcca cgccgtctct tctctcggtt tgtccgccat ttacgatttc aaactcaacg
      301 ataaattcaa accctatatc ggcgcgcgtg tcgcctacgg acacgttaaa catcaggttc
      361 attcagtgga aaaagaaacc gagattgtta ccactgtacc aacgggaggc gctcaaacgg
      421 gaggcactat cctacaaggt ccgaccccca aacctcccta tcacgaaagc aacagcatca
      481 gcagcttggg tcttggtgtc atcgccggtg tcggtttcga catcacgccc aagctgactt
      541 tagacaccgg ataccgctac cacaactggg gacgcttgga aaacacccgc ttcaaaaccc
      601 acgaagtctc attgggcatg cgctaccgct tctgattccc cgataccgat gccgtctgaa
      661 ccttcagacg gcatgagacc tttgcctgcg tacttggtgc gctggtcgcc tccgaacatg
      721 gcgcgacacc cgacatttcc gccgaacgca tcgggcgttt catgaatccg gtttaaaacg
      781 catggaaaaa tgccgtctga aagcctttca gacggcattt tgcttgagat tccgtttacc
      841 aatgactgac aaacgcttcc aaatcggtat tcttgggctt atgcacttcc tctgtcggcg
      901 tgccgaccat catcagcccg atgattttat ccttatccgc acatccgaaa gcctcacgca
      961 acagggggct gttgacccac atccccgtaa tccagacatt gtcgaaacct tgcgccgttg
     1021 ccgccagttg cagcgcatac gccgcacaac ccgccgtcag catctgctcc cattccggtt
     1081 tcggcttagg cacatcgcgg ttcggcgcaa acgttacccc gataaccatc ggcgacatat
     1141 tgcccacttt ttccgccttt ttcatcgcat cgtcgccgaa attcaattcg gcaaccgttt
     1201 gctttaacac atcgcgaaaa cgttgcaatc ctacctcgcc ttgaatcacg ataaaacgga
     1261 aggggcgcat attgccgtga tcgggaactt gggttgccgc ctgaaatatt tgttccaact
     1321 ccgccgcatc gggggcgggg ggcttcagct ttttggaaga tcggcggttc gtcaataatt
     1381 ttaaagcatc catatcgtta tactccggtc atcggtcggg tgttcggaca catatgccgt
     1441 ccaaaggctt cagacggcat atccggcatc agcgcggacg gcggcaggct gccaatatat
     1501 ccatttcctt ccgataggtt tgactgttgg aaatgtccat cagccccaat accgtgctga
     1561 aatagtggtc gtgcgaatat tcgttttccg ccgctttttg tttgaggcat tggaaatcta
     1621 tgtccccgtg ttggcggaag gctttggaaa accacataac catcgggata tgcgtctgcc
     1681 cagaaggcgc gatggcgtaa ggcgcggcgt gcaggtacat cccgttttcg cccaaacttt
     1741 cgccgtggtc ggaaacataa tgcaccacgc tttccaaatc gtcgcggttt tcaagtttgc
     1801 ggataacctt gtcgataaac tggtccacat acaaaaccgt attgtcgtaa gtgttgacca
     1861 gcgtggcgcg ggtgcatttg ttgatttcgt tggtgtcgca ggtcggcgtg aatttgcgtt
     1921 cggcttcggt atagcgttcg taatacgtcg gcccgtggct gccgatggta tgcaggatta
     1981 aaaccgcgtc tttatcgttt ttgttgagga cttcgtcgaa cttagtcagc aggatattgt
     2041 cgaggcactc gccgttgcgg cagtattcgg gcaggttgag cgaggtaacg tcggtattcg
     2101 gcactttgcc gcacacgccc ttgcagccgg aatcgttttc caaccaggta acttccacgc
     2161 cggcgcgctg cacgatgtcc agcaggttgt cttggtgttc ggctttgatt tcgtcataat
     2221 ccgtgcggtc gaaggttgag aacatacacg gcagggagtg cgcggtcgat gtgccgcagc
     2281 ttctgacctg cgggaaattg acaatttcat cgccgcgcgc ggcaagcagc ggcgtagttt
     2341 ggcggctgta accgttcaaa ccccagttgg cggcacgcgt ggtctcgccc acgaccagca
     2401 ccacgaaacg gcgcaggctg ccggccggcc ggttttgcac gaccgccata tccaattgcg
     2461 tataaggaat attggaacgc ttccaatctt tgtatttcga cacgcccgcg ccgatgaaat
     2521 tagacggcac aatcagatgg gttactgatt tattgttgcg gaaaaacgag gcgtaatcct
     2581 gatattgcaa cattgcgata cccaacgcgc acaaaaagga aacggcggca agcacaaggc
     2641 gcgtcaaaag ctccttatac caaacgcggt atttaacctt gacggcgata tacgccagcg
     2701 cgggcaatac gcccaaacat acaatccaca gcacatagcc cggcgtaatc aggcgcgcgc
     2761 tttcggcagc cgtagtttgc aagacattat tcaacatcga cttgttgaaa tagatattga
     2821 aaaatatttc ttggtaagac accgccgcac tgataaccaa tatcaacgga atcaatacct
     2881 tatgcacgaa aggcagggca atgacgtgaa aaacgaaatt acttaaaaaa aacagcacca
     2941 ccggcatcgt atagaggaag atatccgccc cggtgccgtt aaaaggatga agctcgacaa
     3001 ctttggcaaa aaaggcgtaa ttcaatacca gcgaggaata cagggaaagg aaggcaatca
     3061 gcgcggaaga gccgagcttc ggcctcaggt tcggttttat catttggaat gtgtcggata
     3121 agggttggaa aaggcatccg gcatttggaa tccggattat tgaaaaagat tcttaattat
     3181 aaggcaacgg agcaaagcag ggcaagaaaa cggcggctgt gcgggggggg tccgcccgcc
     3241 attcaaacgt ccggcagaca taaaaacatc gtaagcaaga ttcgaaccgg tctgcaaccg
     3301 cccctgccag aaaaacgggc aaagcatttc atattggaaa aacccggccg cgccgccgac
     3361 gggacagtcc ggcacaaaca gcatcacgcc cggattgaaa aggacggata gccgccggca
     3421 gcacagtttt accccgcatc ccaaaacgcc gccggagaac gccgaaacag ccgatgccgt
     3481 ctgaagccgc ttcagacaac atcgggacat caaccgtaac gccgttggaa atcgcgcata
     3541 aaatctgcca aagcccgcac gccttcgagc ggcatcgcat tataaatgct ggcacgcatc
     3601 ccgccgacag acttatagcc cttcaacagg cacaagccct gcaattcggc ttccagcaca
     3661 aaacggcggt caagctcctc atccctcgtt tggaacacga cattcatttt agaacgcgca
     3721 ttcggacgga tacggttgat atagaaaccg ccgctgccgt ctatcgtctc atacaaggtt
     3781 tgcgccttca gcctattgac cgcttcaatt tttttcacac cgccctgcgc ctgcagccag
     3841 cggaacacca gccccgacat ataaatcgcg taagttgacg gcgtgttgta cataccgtcg
     3901 cggttgatgt gcgaacggta gttgaacaca tcgggaatat cgttcggaca acgctcgagc
     3961 aaatcctcac gcacaatcac caccgtaact cctgccggcc cgatgttttt ctgtgcgcct
     4021 gcgtaaatca gtccgtagtc ggcaacatca aactcgcgcg acaaaatctc gctggacata
     4081 tcgcacacca gcggcggcat gccttctgaa aggcgcggca cttcacggta ttgcagcccg
     4141 ttgaccgttt cattgacggc aaaatggaca aacgccgaat cgggtgcaac atcccacgtt
     4201 tccacaggcg gcaggtcgag atagtcgaac tgctcgccgc catgcgccgc caaacggatt
     4261 tccgtatcgg tcaaacggct catctgttca taagcgatac ggctccagtt gcccgttacc
     4321 accgcgtcgg cagtgcggaa accgtgtgcc agattcatgg ctgccatatt aaattgggtt
     4381 gttgctccgc cctgcagaaa caatatctta tagttgtcag gcactttcaa aagctgcctc
     4441 aaatcctgtt ccgcatgatg caggatgctc aaaaacattt ccgaacggtg gctcatcgcc
     4501 atcacgggaa aacccgtacc gttgtagtcc aacatttcct gccgcgccgt ttccaacacg
     4561 gcttcgggca atacggcagg gccggcggaa aaattgtaaa tcggataaag agacatgatg
     4621 cagccttgat tctgaacaat aacccgcctg attttaggct tgcagcaggc tttgtgcaag
     4681 gatgggaaat acctgtcctc cgccccgctt catgccgccc gaacacggaa aacaacatca
     4741 atatattgat ttacaaatat aaaaatcatg ctagcgacaa atagatacat ttgttttgtc
     4801 aacaatattc acgatttccc attacaaacc tcccttacac ccgctttttt ccgtcccaaa
     4861 aacacaaaat aaatcaacac tttcatttct ccgcaaaagc ggttataatc acgccgattt
     4921 ttcaactttg acgaaaaatg gccacggggc cattttttta ttatgtacat agggagcagc
     4981 atggatatcc aaaccatcct cgaaaaaacc ctgcccggcc tgggctacga actggtcgat
     5041 ttcgaactga ccgcgcaagg aacattgcgc gtgttcatcg acaaagaaag cggcattacc
     5101 gtcgaagact gcgcaaccgt cagcaaccac ttgagccgcg tcttcatggt tgaagacatc
     5161 gactacaaaa acctggaaat ttccagcccc ggactcgacc gccccttgaa aaaagccgcc
     5221 gacttcgtgc gctttgccgg tcagaatgcc aaaatcaaaa cccgcctgcc gatagacggt
     5281 cagaaaaact ttatcggtaa aatcgaaggc tgcgaaaatg ataccgttac catatccttc
     5341 gacggcaaaa ccgtacaaat cgaattgggc aacatcgaca aagcccgtct gcgccccgaa
     5401 ttcaaattct aaaacacagc aatattggag atgttcaaaa tgagtcgtga aatgttacag
     5461 ctggcagaag cactggcaag cgaaaaaaac gttgatgcgg aagtcgtctt ccaagcactg
     5521 gaattcgccc tgtctaccgc cgccaagaaa aaggcagacc gcgaacacat ggatgtccgc
     5581 gtacaaatca accgcgacac cggcgaatac caaaccttcc gccgctggct gattgtcgcc
     5641 gatgaagact atacctatcc cgatgtcgaa aaaaccatcg aggaaatcca agaggaaatt
     5701 cccggcacta ccatccaaat cggcgaatac tacgaagagc agctgcccaa cgaaggcttc
     5761 ggccgccaag ccgcgcaaac cgccaaacaa atcatcctgc aacgcatccg cgatgccgag
     5821 cgcgagcaaa atctgaacga gtttctcgcc gtcaaagaag acatcgtgtc cggcacggtc
     5881 aaacgcgtcg aacgccacgg catcatcgtc gaagtcgttg ccggcaaact ggacgcgctg
     5941 attccgcgcg accaaatgat tccgcgcgaa aacttccgca gcggcgaccg catccgcgcc
     6001 ctcttcctgc gcgtcgaaga aatcggcaac accggccgca aacaagtcat cctgagccgt
     6061 acttccggcg atttcctcgt caaactgtac gccaatgaag tacctgaaat tgcagacggc
     6121 atgcttgaaa tccgcgctgt cgcccgcgac ccgggacaac gtgccaaagt cgccgtcaaa
     6181 gccaacgacc agcgcatcga tccgcaaggc acctgtatcg gcgttcgcgg ttcgcgcgtc
     6241 aacgcggtca gcaacgaatt gtccggcgaa cgcatcgacg ttgtcctttg gtcgcccgag
     6301 cctgcccaat tcgtcatgag cgcgctctca cccgccgagg tcagccgcat cgtcatcgac
     6361 gaagacaaac acgccgtcga tgtcatcgtt gccgaagacc agctcgcgct cgccatcgga
     6421 cgcggcggtc aaaacgtgcg ccttgcttcc gacctgactg gctggcagct caacatcatg
     6481 acctctgcag aagcagacga gcgcaatgcg gcagaagatg ccgccatccg ccgcctgttt
     6541 atggattact tgaacgtgga cgaagaaacc gccgacgtac tggttcagga aggttttgca
     6601 accttggaag aagtcgccta tgttcctgcc gccgaactgc ttgccattga aggatttgac
     6661 gaagaaatcg tcgatatgct ccgcaaccgc gcccgcgatg ccatcctgac catggcgatt
     6721 gccgccgaag aaaaactggg cgaagtatcc gacgatatgc gcaacctcga aggcatagat
     6781 gccgatatgc tccgcagcct tgccgaagca ggcattacca cccgcgacga cttggcagag
     6841 cttgctgtgg acgaactgat tgaaatcacc ggtgtaaacg aagaaaccgc aaaagccgtc
     6901 atcctgaccg cacgcgaaca ctggtttacc gaagacaaat aaagggggta cagatgagta
     6961 acacaaccgt agaacaattt gccgccgagc tgaaacgccc cgtcgaagac ctgttgaaac
     7021 agttgaaaga agccggcgtc agcaaaaaca gcggcagcga ttccctgacg ctggacgaca
     7081 aacagcttct gaacgcctac ctgaccaaga aaaacggcag caacggcggc accatcagca
     7141 tccgccgcac caaaaccgaa gtcagcaccg ttgacggcgt aaaagtcgaa acacgcaaac
     7201 gcggacgcac tgtcaacatt ccttctgccg aagaattggc agcacaggta aaagccgccc
     7261 aaacccaagc cgcacctgtc cggccggagc agacggcaga agacgcggca aaagcccgag
     7321 ccgaagctgc cacacgtgca gaagcccgtg ccaaggcaga agcggaagcg gcaaaactga
     7381 aagcggcaaa agcaggcaac aaagccaaac ctgccgcgca gaaacccacc gaagcaaaag
     7441 ccgaaaccgc acccgttgcg gcggaaacca aacccgccga agaaagcaaa gcgggaaaag
     7501 cccaagccga caaaatgccg tctaaaaaac ccgccgagcc caaagaaaaa gccgccaagc
     7561 cgaaacacga gcgaaacggc aaaggcaaag atgccaaaaa accggcgaaa cctgccgcac
     7621 ctgccgtgcc gcaacccgtg gtcagcgcgg aagaacaggc gcaacgcgat gaagaagcac
     7681 gccgtgccgc cgcacttcgc gcccaccagg aagccctgtt gaaagagaaa caggaacgcc
     7741 aggcacgccg cgaagccatg aaacaacagg cagaacaaca ggcaaaagcc gcacaggaag
     7801 ccaaaaccgg cagacagcgt cccgccaaac ctgccgagaa accgcaggca gccgcgccgg
     7861 ccgtcgaaaa caaacctgtc aatccggcaa aagcgaaaaa agaagaccgc cgcaaccgcg
     7921 atgacgaagg tcaaggccgc aacgccaaag gcaaaggcgg aaaaggcgga cgcgaccgca
     7981 acaatgcacg caatggcgac gacgagcgcg tacgcggcgg caaaaaaggc aaaaaactca
     8041 aactcgagcc gaaccaacac gccttccaag caccgaccga acctgtcgta cacgaagttt
     8101 tggtgcctga aaccattacc gttgccgact tggcacacaa aatggcagtc aaaggcgtgg
     8161 aagtggtcaa agccctgatg aagatgggca tgatggttac catcaaccaa tccatcgacc
     8221 aagacaccgc cctgattgtc gtcgaagaac tcggacatat cggtaaacct gccgcagcgg
     8281 acgaccccga agccttcttg gacgagggcg cggaagcagt ggaagccgaa gcattgccgc
     8341 gtccgcccgt cgttaccgtg atgggccacg tcgaccacgg caaaacctcg ctgctggact
     8401 acatccgccg taccaaagtg gtacagggcg aagcgggcgg cattacgcag cacatcggcg
     8461 cgtaccacgt tgaaacccct cgcggcgtga ttaccttctt ggacaccccg ggccacgaag
     8521 cctttaccgc tatgcgcgca cgcggtgcga aagcaaccga catcgtgatt cttgtggtcg
     8581 ccgccgacga cggcgtgatg ccgcaaacca tcgaagcgat tgcccacgcc aaagcggcag
     8641 gcgtaccgat ggtggttgcc gtcaacaaaa tcgataaaga agccgccaac ccagagcgta
     8701 tccgccaaga gctgaccgca cacgaagtcg tgcctgacga atggggcggc gatgtacagt
     8761 ttatcgacgt ttccgctaaa aaaggcctga acatcgatgc attgctcgaa gcagtcttgc
     8821 tcgaagccga agttttggaa ctgactgccc ctgtcgatgc acctgccaaa ggtatcatcg
     8881 ttgaggcacg tttggacaaa ggccgcggcg cggttgccac attgctggtt caaagcggta
     8941 cgctgaaaaa aggcgatatg ctgctggccg gtacggcatt cggcaaaatc cgcgcgatgg
     9001 tcgatgaaaa cggcaaatcc attaccgaag ccggcccgtc catccccgtc gaaatcctcg
     9061 gcttgtccga cgtaccgaat gcgggtgaag acgcgatggt attggcggac gagaaaaaag
     9121 cgcgcgaaat cgccctcttc cgccaaggca aataccgcga cgtgcgcctt gccaaacagc
     9181 aggcggcgaa gctggaaaat atgttcaaca atatgggcga aacccaggcc caatccttgt
     9241 cggtcatcat caaggcagac gttcagggtt cttacgaggc tttggcgggc agcctgaaaa
     9301 aactgtccac cgacgaggtg aaagtgaacg tattacacag cggcgtgggc ggcattaccg
     9361 aatcggatgt caaccttgcc atcgcgtccg gggcgttcat tatcggcttt aacgtgcgtg
     9421 cagatgcctc ttcgcgcaaa cttgccgaaa atgaaaatgt ggaaatccgc tactacaaca
     9481 tcatctatga tgccatcgac gacgtgaaag ccgccatgag cggtatgctt tccccggaag
     9541 agaaagaaca ggttaccggt acggtcgaaa tccgtcaggt catctccgtt tccaaagtcg
     9601 gcaatattgc aggctgtatg gttaccgatg gtgtggtcaa acgcgattcc catgtccgcc
     9661 tcatccgcaa caacgtggtt atccataccg gcgagctggc ttcgttgaaa cgctataaag
     9721 acgacgtaaa agaagtccgc atgggctttg agtgcggtct gatgctcaaa ggctacaacg
     9781 agattatgga aggcgaccaa ctggaatgct tcgacatcgt cgaagttgcc cgcagcctgt
     9841 aattcctttg caaataaaat gccgtctgaa gccttcagac ggcatcggaa aacgggttct
     9901 gtatcataca gaacccgttt tttgtcgcaa atcggcttca gcgaccctcc tgccttatcc
     9961 cgatttgaat ctgacttgcc atacaaacag gcttcagacg gc
//
LOCUS       DAXHNE010000063         9860 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.063, whole genome shotgun sequence.
ACCESSION   DAXHNE010000063 DAXHNE010000000
VERSION     DAXHNE010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9860
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.063"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(137..832)
                     /locus_tag="ACFM4Z_001183"
     CDS             complement(137..832)
                     /locus_tag="ACFM4Z_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC6021222.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSVQSSSSAPAAPVE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKLLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
     gene            complement(947..3058)
                     /locus_tag="ACFM4Z_001184"
                     /pseudo
     CDS             complement(947..3058)
                     /locus_tag="ACFM4Z_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(3244..3849)
                     /locus_tag="ACFM4Z_001185"
     CDS             complement(3244..3849)
                     /locus_tag="ACFM4Z_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC6021223.1"
                     /translation="MTRQSLQQAAESRRSVYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAVVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            complement(3898..4296)
                     /locus_tag="ACFM4Z_001186"
     CDS             complement(3898..4296)
                     /locus_tag="ACFM4Z_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC6021224.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            4404..5315
                     /locus_tag="ACFM4Z_001187"
     CDS             4404..5315
                     /locus_tag="ACFM4Z_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6021225.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTGAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            5305..6606
                     /locus_tag="ACFM4Z_001188"
     CDS             5305..6606
                     /locus_tag="ACFM4Z_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HFC6021226.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCAAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGQTGIPIIDAAMRCLHKTGGLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTYPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPAYPVAGIP"
     gene            complement(6674..6844)
                     /locus_tag="ACFM4Z_001189"
     CDS             complement(6674..6844)
                     /locus_tag="ACFM4Z_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021227.1"
                     /translation="MLLYRLLGGAEIECGRCPVPPMTDWQHFLLAMGTVSAWVAVIWA
                     YLIIESEKNGRY"
     gene            6998..9148
                     /gene="dinG"
                     /locus_tag="ACFM4Z_001190"
     CDS             6998..9148
                     /gene="dinG"
                     /locus_tag="ACFM4Z_001190"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFC6021228.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LTREEGGDPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELSFALAKGRGRYLCPYKLYQLTQNNAQQNLTGFETPEVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQMTGKIAALTDKAELANLADETAASLLDSLHEWQFHLAE
                     EPSLSLGVSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVIGLNDALS
                     AARREKEQDSALLDRLTGEFGLFIARIEQISAVWDLLSTVPLEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLILRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAIIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            9184..9798
                     /locus_tag="ACFM4Z_001191"
     CDS             9184..9798
                     /locus_tag="ACFM4Z_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFC6021229.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIGDIAAAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGF"
BASE COUNT         2316 a         2882 c         2527 g         2135 t
ORIGIN      
        1 ggtttaaatt taatccacta tatattctga tttaaaacca taaggcttta agcaatcatc
       61 tcttctataa agcctaaata caaaaggccg tctgaaatcc tattttcaga cggcctttac
      121 tcattcaatc ctcaacttat tgcggctttt tctgctcttc gcgtacttta tccaccaact
      181 ggtcaatcgt ggtcataccg gactgccagt ctttaaattc aacttggtat ttgccgccga
      241 cgatcacagt cggtgtgccg ctgatttgga atttattggt caactcttcc atttgagccg
      301 cacgcgcttg gctttcagga gcctcaaatg cagccaataa ttttttgccg tcaaacgctg
      361 tttgctcgga cagccatttt ttcagggtat cggtatcggc cagattgatt ttttgattaa
      421 ccatcgcatc gaaaatatgg ctgttggctt tatctgattc accggccatt tccactgcgg
      481 ccgccaaacg tgccaaaggt ttcatttcat caccccacac gacatgctcc cggcgcatat
      541 aggtatcgtc tttaaacgtt ttgatgtgct cgctcaagac cggctcaaga tgggcgcaat
      601 gcgggcagaa gtagccgaaa aattccaata cttcgatttt accggcctgc tgttgcggaa
      661 taggcgtaga caatacagtg tagttcacac cttcgttcaa ctcaacaggg gctgccggag
      721 cagatgagct gctttggacg ctgtctgccg gaacgctggt ttcagcctgt ttgctacaag
      781 cggccaatgc caacagggtc aatgaagtca aagctaaggt tttcagtttc ataggtatct
      841 cttgtgtgtc agattaatgt gcggatttta tgacatttta ttgaaggcgt gttcgatttg
      901 agtgaaaaaa tgtgtttagt tttatttcag cctttgcctg atttcatcag aaacggtaat
      961 ttacccgggc agtatagccg cgcggattac ccggcataga gtccgaacgc caatattttt
     1021 gattgaacag attggctgcg gcaaaggtaa cgttaacatt tttatggttc cagccaagca
     1081 tggcatcaac tcgggcaaag cctggaagcg tagtcacttc tttatttctt gagttgtaac
     1141 cgtagcgttt gcctgtaccg gttacgccga tttcgccgta gaggttttcg gtcggggtat
     1201 aacggaaaaa caggttgccg gtaacgttgc tggtattatt caaatggatg cccactcggt
     1261 cgggattttc tttgtcttca acgactttcg cctgcatcac gcccaacgaa ccgcgcagat
     1321 agagtttttt ggggatgatt tgcccgatgg cggacaattc cacgccgcgc gaacggtgtt
     1381 tgccgccgac tgcccaagtg tagggatcat tttgctcgtc ggggcggtag cggatattga
     1441 agcgttcgat ttggtaggca gacaacgtag tgctgaggcg gtcgtccagc caactgcttt
     1501 tcacaccggt ttcgtattgg cgggtgtact cggggtcggc gttgaacacg gcggaagacg
     1561 acgtgttgat gctcaaatag ccgccgcgtc cgccataagg cgcgaagcct ttattatacg
     1621 aggcgtaaag tgtgtggacg ggattgatgt tccacactgc gccgatgttg gggctgaacg
     1681 agtgtccgct gtattggcgg ctgctgccgg tgagtttgtt ttcggaatta aaggtgtatt
     1741 tgtcgtaacg accgccgagg acgaatttca aatcgggcgt ggcggagaag atgttttgca
     1801 caaagatgcc gtaggagtcg gctttgtggc ggttttgggt cagaataggc tgcaatctgc
     1861 ccgaagccgg ccagcttgcg cggtcgtagg ggttgatgga ggcggaaaag gcgctgctga
     1921 aacccaatgt cgggttgcgg tgttcgcggc tgtaatccat gcctacggtc aggtggtttt
     1981 caaaacggcc gatggtgtag tcgccgttga gcgtgaagtt ggacgacagg gttttgttgt
     2041 cggtctgctg ctaggcgtag ttacgtttaa ttaggctgcc gttttcgctg cctgcataga
     2101 aatgatcaaa atcctgcgcc gccgtgcggt gggcgagctg ccattgggca cgccatttgt
     2161 cgttgaaggc gtattcaagg tcggaacgcc aaacttgcag cttgtctttg acaaaatcgt
     2221 tccggtgggc gaaccccatg cggtaaggca gtccgaagcg gtcgtacacg gacttggtcg
     2281 gactgcggtc gggcgtgcgc tccacattgt cgtaggtgta ttgccccgtc cacttcaagc
     2341 cgttgtcgag tttgacggta atactgggcg aaaccatgac atttttgctg tctatgccgc
     2401 tgcggaacga attggcgcgc ccgacttcgc cggtaagacg gatggcgacg tttttgttca
     2461 gcacttcgtt aatgtccata ttcaggctgc ggtttgccca cgaaccgtaa accgcaccga
     2521 tgttgcggct ttgtttgaag ttggcgtatt tgctgaccat gttgatgacg ccgccgccgt
     2581 tggtgcggcc gtaaagcacg gaagacgggc ctttcaggat ttccacgcgc tcgatgttgg
     2641 cagtactgcg gcgcacttgt ccgctttcgc gcacgccgtc gcggtaaata tcggatgcgt
     2701 cggcttgaaa accgcgcagg aaaatgcttt cgccgcgcat atcgtaggca gcgtcgatgc
     2761 cggcattgcc ttcgaggatg gaactcaaat cgttcgtacc gtaatttttg tttttctgga
     2821 tattgagcgt atcgatggtt tgcggcgttt ctttgatgag ctgtccgttg cgggtaacgg
     2881 cggcttcgtc gtagttgatg tagcctttga gtacgctggt gtcggactgt ccgaccacgg
     2941 aaacggtggg cagagtggcg gtgtaatgtt caccattgtc ctgcgtatcg gcggcagcaa
     3001 cagggaagga agcaataatc agcgtgggta ataaagctaa atgaaatgat attttcattt
     3061 ttatactcaa tttaacaaaa caaccgaatt atattgcctc acggaggaaa tgagaataat
     3121 ttcttttaac tatattgaac atgatatttg taaacaaagg tctcagaatg cggaaaactc
     3181 gccgcctgat actgaaaaat gccgtctgaa cagggttcag acggcatttt tttgaccgcg
     3241 aaattatgcg ccgaacactt tcaaacgttc tgcaactggt tcaaaggtct tttcacctgc
     3301 cgccccttca ataccgccga taaccatttg tgcgcgcaac aaccagtttt cggggatatt
     3361 ccacgctttg gcaatcgccg catcgggcaa gggattgtaa tgttgcaggt ttgcacctac
     3421 gccgaccgcg gcaagtgtcg tccaaacggc atactgcacc atcgcgttcg cctggtccgc
     3481 ccaaacggga aagttggcgg cataagcagg gaactgctcc tgcaaacctt tgacgacatt
     3541 ttgatcttca taaaacaaaa tagttgccgc acccgcctta aacaggttca atttttgcgc
     3601 ggtcggttca aaactgtcgg caggcacgac ggcacgcagc gcgtcttcga caaattgcca
     3661 caccttatca tgctcttcgc caaacagcac gaccacgcgg gcagattggg aattgaacga
     3721 agaaggtgtg tgcaaaacgg cgtgttcgac gatttggaca acttcatctt tgccgacggg
     3781 caggttttta tttaacgaat aaacggaacg gcggctttcg gcagcctgtt gcagagattg
     3841 acgggtcatt tcttttcctc aaaaacattc aacaaaatac actttaggca gtccggttca
     3901 ggctttacgc ttgaaaaaca gcctgtccag cgaccatgct ccgccgcccg ctgccgcgat
     3961 atagaggaat acgaagcaga acagcactgc ggactcgccg ccgttggcaa tcgggaacaa
     4021 agcatttccg gaagcgtgcg ccataaaata ggcaaccgcc atctgaccgg acaaaacaaa
     4081 cgcggcaggg cgcgcaaaca ggcccaacac cagcaaaatg ccgccgacaa tttctaaaat
     4141 accggcaagc agcaacagcc cgccgggcga accgctgccc atttcaatgg ggaaagcgaa
     4201 gattttcgac gtaccgtgca acaaaaacag gtaggcggtt acgatacgca aaacagaaag
     4261 caaaaccggt tggatacggt tgcagcaatc ggacataaaa gctcctttga tatttgtctt
     4321 catttcagac ggtagagtat agatgtttcc ccttttcaaa tatatactgc ttttattgat
     4381 acaacttttc cacacaagaa aatatggaca ccctgttcag cctcaaggtt ttccgccaag
     4441 tcgtccaaag cggcggcttc acccgcgccg ccgacgcgct cggcatctcc accgcaatgg
     4501 caagcaaaca cgtcagccac ttggaaaaca ccgtccaagc caaactcctg caccgcaaca
     4561 gccgcaacct cagcctgacc ggagccgggg aagaatacta ccggcaatgc agttacgcgc
     4621 tcgacacgct cgacgatgcc gcgcaaaaag ccgccggggg gacggaaaaa ccgcaggggc
     4681 tgctgcgcgt aacgatgccg ctgtggtttg ccggcagcca gatatgcaac tggctggcgg
     4741 aataccgcga acgttatccc gaagtaacat tagaactgat tttggacaac cgccacgtcg
     4801 atttgattgc cgaaggcgtg gatttggcgt tgcgcgtttc ccaaaccctg tccccgtcgc
     4861 tgattgcgcg cccgctggcg gaaatcgaat ttgccctgct cgcctcgccc gatttcctga
     4921 ggcgcaacgg cgtgccggaa acgccggaag aagtggcagg gctgcccgcc gtcctgccga
     4981 cctacaccaa ccagcagaaa ctcgacctca cccgcaaatc ggacggcaaa aaataccggc
     5041 ttgaactgac ccccgtcatc cgtaccgaca acacgctgat gatgcgcgaa atgattaagg
     5101 cgggcgcgtg catcggttat cagccgcttt gggcggcgga acacgatttg cgctgcggca
     5161 cgctggtgag gctgctgccc ggatacgccg tcccgaccga ccggctgaat gccgtttatg
     5221 cagacagggc attcttaagc gcgaaagtcc gcagcttcat cgattttctg aacgaaaaaa
     5281 tcgccagcag gaaaggctgc cgaaatgccg tctgaaaccg cccgccccct ttatgcggac
     5341 acgtgcgccg cacacacgct cgtctggttc cgccaaaacc tccgcatccg cgacaacgcc
     5401 gccttatgcg ccgccgttgc cgaaggtttg cccgttatcg gtatttggat tgacgatgcc
     5461 gaaacagaca accctcgccg cgccgcgttc taccgccaat ccgccgccga actcgcccaa
     5521 gggcttgcag ggcgcggcat cccgctctac acggcggcat cgcctgccga gctcgtccgg
     5581 ctcgccgtcc gcctcaatat ccgcgccgtc atcgccgacg aatcccatac ttttgccgac
     5641 aaactcgccg acaacgccct ttggcatgaa ttggacaaac acggcatcgc gttaaccttc
     5701 gtcaacgacc gtgccgtttt cggcaaaacc gacctgatac ccgacggcgg cacggcacac
     5761 accgatttcg accgctaccg cgaagtatgg ctcgaccgct tttccaagca gccccccgcc
     5821 ggtccggacc tattcgcggc ataccgccaa cccttccccg aaaacctttc cgccccgccg
     5881 cctgccgccc tttcagacgg catcttcctg ccgcaaaaca gcggcgaaac ggcggcttgg
     5941 cggcagtggc ggcggtttct cgaacaggcg gattcctact ccgttttaaa ggatttcccc
     6001 tcgcgcaaac acacttcgct gatgggcgcg tatttgagtg ccggctgcat ctcgccgcgc
     6061 ctgctcgcgc gggaaagcct cgaacgccgt ctgaacgcgt gggcggacaa catcatccgc
     6121 cgcgattttt tccttcaact tgccttgcag cacacggatg acgacccttc agacggcaat
     6181 ccggaacaca ccctgcgcct gacgctttgg cagcagggcc agaccggcat tccgattatc
     6241 gatgccgcga tgcgctgttt gcacaaaacc ggtggcctcc accccgccct gagacgcttg
     6301 agcgcggact ttttctgcca cgttttaaac ctcccccgcc gcgaaggcga gatatggttt
     6361 gcccgacagc tgaccgattt cgatgcagca atcaaccaag gcaactggcg gcttgccgcc
     6421 tcacggcaca cctaccccga cattgccgcc gcctcatacc gtaccgaccc ggacggtact
     6481 tttatcaaac ggcacattcc cgaactcgcc cacctgtccg ccgacaccgt ccacacgcct
     6541 tggcggtttg cctgttcggt cgatacccac ggctatcccg cctatcctgt cgccggtatt
     6601 ccctaagcgg tacggcaacc taaaacaaat gccgtctgaa cttccgtttc agacggcatt
     6661 gcgtccggat gactcaatat cttccgtttt tttcactttc aataatcaga tatgcccaaa
     6721 tcaccgccac ccaagccgac accgttccca tcgccagcaa aaaatgctgc caatccgtca
     6781 tcggcggtac aggacaacgg ccgcattcga tttccgcgcc gcccaacaac cggtacagca
     6841 gcatatccgc aatacaaagc aacaacgggt atttcaaacg ctgcaaccaa aaaaccaacc
     6901 acgtccgcac gatttacctc ccccattccg acaacgatat tgtaccgctt ccgtgcaatg
     6961 ttaaaataat cccacttcta ccgccgtaca aaacgctatg ctcaccgatt tagaaaaaaa
     7021 cgccatccgc gaccattacc aaaatatcgg caaaaatctg cccggtttcc gtccgcgcgc
     7081 ttcgcagcgg gaaatgattg cggcggttgc caacgctttt tcgcggacgt tgacgcgtga
     7141 agaaggcggc gatccaccca agcgcgaggg cgagagcatt gccgtaatcg aaggcccgac
     7201 cggcgtgggc aaatcgcttg cctacctgct ggcgggcggc atcatggcgc aaacacgcgg
     7261 caagcggctg attgtgagca gcgcgacggt tgccttgcag gagcagttgg tagaccgcga
     7321 cctgccgttt ttggtcgaaa aaagcggttt ggaactgagc ttcgcgcttg ccaaagggcg
     7381 cggccgttat ctctgcccct acaaactcta tcaactgacg caaaacaacg cccagcaaaa
     7441 cctgacgggt tttgaaacgc cggaagtgtt gtgggacagc aaacccaagc ccgaagaatt
     7501 gaagctgctg cgcgacatcg ccgacgaatt ttccgcccga cggttcaacg gcgaccgcga
     7561 cacttggccg gaaaaaatcg atgacgcgat ttggctcaaa gtgaccaacg accgccacgg
     7621 ctgcctgaaa accgcctgtc ccaaccgtcc ggaatgccct ttttacctgg cacgcgatgt
     7681 cttggaaacc gtcgatgtcg tcgttgccaa ccacgatctt ctgcttgccg acatcagcat
     7741 gggcggcggt gtaattctgc ccgcgcccga aaacagtttc tattgtatcg acgaagcgca
     7801 ccacctgccc aaaaaagccc tcagccgttt tgccgccgaa cattcatgga atattgccgt
     7861 ttggacgctg gaaaaactgc cgcagatgac cggcaaaatt gccgcgctga ccgataaagc
     7921 cgaacttgcc aaccttgccg acgaaaccgc cgcatcattg ctcgacagcc tgcacgaatg
     7981 gcagttccat ttggcggaag agccgtcttt aagtttgggg gtgtctgaaa acgacagacg
     8041 aaccaacagc gaaccgactt ggctgtggga agacggcaaa atccccgaag gcctcgaaac
     8101 caccgtttcc aacaccgcca ttgccgcacg cagcctgctc aaacacgtta tcgggctgaa
     8161 cgatgcgctt tccgctgcgc gccgcgaaaa agaacaagac agcgcgctcc tcgaccgcct
     8221 gaccggcgag ttcggtcttt ttatcgcccg tatcgaacaa atcagcgcgg tttgggattt
     8281 gctctccacc gtccctctcg aaggtgaaga accgctggca aaatggataa cccgccgcgc
     8341 cgacgacaaa aacgactaca ttttcaacgc cagccccatc agcagcgcgt cccacctcgc
     8401 caacagtctg tggcggcgcg cggcaggcgc ggtgttgacc tccgccaccc tgcaatcctt
     8461 gggcaacttt aacctgatat tgcgccaaac cgggctgcaa tggctgcccg aaaccaccac
     8521 cctcgccctc aaaagcccct ttgactttga aaaacagggc gaactctaca tccctcccat
     8581 atacgccagc cccaaagacc ccgaagccca caccgccgcc atcatcgaat ggctgcccaa
     8641 gcttatttcg cccaccgaag ccatcggcac gctcgtcttg ttttcctcgc gcaaacaaat
     8701 gcaggatgtc gccctgcgcc tgcccggaga ctacctgccg ctcttgctcg tacaaggcga
     8761 attacccaaa gccgtcctcc tgcaaaaaca ccaccgggcc atagaagaag gcaaagccag
     8821 catcatcttc ggactcgaca gctttgccga aggactcgac ctgcccggca ccgcctgcgt
     8881 gcaagtcatc atcgccaaac ttcccttcgc catgcccgac aaccccatcg aaaaaaccca
     8941 aaaccgctgg atagaacagc gcggcggcaa ccctttcatc gaaatcaccg tccccgaagc
     9001 cggcatcaaa ctgatccagg ccgtcggccg cctcatccgc accgaacaag actacggccg
     9061 cgtaaccatc ctcgacaacc gcatcaaaac gcagcggtac ggccaacaat tattggccgg
     9121 cctgccaccg tttaaaagga tagggtaaac ataccgccct tcccgcaaca gaaaggaaaa
     9181 atcatgaact tcacccgact gctcaaccaa gtcttaagca cggttcaaaa aaaaggcaac
     9241 acattctccg acagcccgct caattcattc ggcggaggcg cgctggttgc cggtgtcgcc
     9301 tccatgctgc tgaacggtaa aaaccgcaaa accatcacca aaatcggttc gaccgccgct
     9361 ttgggctacc tcgcctaccg gggctatcag atgtggcagc aaaacaaagg gcgggcaacc
     9421 gtaacacaaa gcgatttcca acctgccgga gaaactgaag aaacatacag ccgcaccgta
     9481 ttgcgcacca tgatagccgc cgccgcttca gacggcatga tagacgaagc cgaacgccgg
     9541 actatcgaac aggaaagcgg cacagacccc gaaactgccg catggctcgc cgccgaatac
     9601 cgcctgcccg caagcatcgg ggacatcgcc gccgccgtcg gcaacgatga ggcgttggcg
     9661 gcggaggcct atctggcggc aaggttggtc tgtgccgatt tgtcgcggaa agaaaccgtc
     9721 ttcctcgccc gcctgtcgca ggctttgaaa ctggatgaca atctggtgga gagtttggaa
     9781 aggcaattgg ggttttgatg acacagccgg ggagggaaaa tcatcaaaaa acggttattc
     9841 cgaccccggc ctcaaaacgg
//
LOCUS       DAXHNE010000064         9687 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.064, whole genome shotgun sequence.
ACCESSION   DAXHNE010000064 DAXHNE010000000
VERSION     DAXHNE010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9687
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.064"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(9..1304)
                     /locus_tag="ACFM4Z_001192"
     CDS             complement(9..1304)
                     /locus_tag="ACFM4Z_001192"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFC6021230.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMEEIEKQVGLERAQWFW
                     QQSLQAVELVDERVRKHAVDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGAQIFEQSPMTRIEP
                     HQNGWLVYTPEGSVECKNLVYAVNTYAGLSPIFKPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDHEFIDDPARMTELVRQDMLKVFPQL
                     ADVRIEYSWGGECDITANLVPHFGRLAANVFYAQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1430..2317)
                     /locus_tag="ACFM4Z_001193"
     CDS             complement(1430..2317)
                     /locus_tag="ACFM4Z_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC6021231.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGACPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAH"
     gene            complement(2317..3282)
                     /locus_tag="ACFM4Z_001194"
     CDS             complement(2317..3282)
                     /locus_tag="ACFM4Z_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC6021232.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTTIDEDLGRLNIAVSYQNYADIFQNFWSTLNPFGDGENSNIYLMTY
                     WSSIKTALTTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3298..4422)
                     /gene="potA"
                     /locus_tag="ACFM4Z_001195"
     CDS             complement(3298..4422)
                     /gene="potA"
                     /locus_tag="ACFM4Z_001195"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HFC6021233.1"
                     /translation="MTATTASSAKPYLKIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLGNG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4697..4966)
                     /gene="rpsO"
                     /locus_tag="ACFM4Z_001196"
     CDS             complement(4697..4966)
                     /gene="rpsO"
                     /locus_tag="ACFM4Z_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFC6021234.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5189..6124)
                     /gene="secF"
                     /locus_tag="ACFM4Z_001197"
     CDS             complement(5189..6124)
                     /gene="secF"
                     /locus_tag="ACFM4Z_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFC6021235.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6128..7981)
                     /gene="secD"
                     /locus_tag="ACFM4Z_001198"
     CDS             complement(6128..7981)
                     /gene="secD"
                     /locus_tag="ACFM4Z_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFC6021236.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQFKVDAALQNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(8193..8459)
                     /gene="yajC"
                     /locus_tag="ACFM4Z_001199"
     CDS             complement(8193..8459)
                     /gene="yajC"
                     /locus_tag="ACFM4Z_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFC6021237.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAISAKVD"
     gene            8587..>9687
                     /locus_tag="ACFM4Z_001200"
     CDS             8587..>9687
                     /locus_tag="ACFM4Z_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HFC6021238.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCQPQKGKIFRL
                     DDDTAISTGSLSAARFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2482 a         2578 c         2417 g         2210 t
ORIGIN      
        1 tgcgacgctt aacggctgtc tttccactga taatatttcg agccgaggaa agaaccgagt
       61 ttgcgcaaaa acggttgcag gataatattc ggctggcgca accgctcaaa cggcttcaga
      121 cggcattcgt cccctaaaat tgcttcggca accgccagac ctgcaatgcc tgttatcgcc
      181 atcccgtgtc cggaataacc ttgcgcataa aaaacattcg cggccaaacg tccgaaatgc
      241 gggacaaggt tggcggtaat gtcgcactcc ccgccccacg aatattcgat tctaacatcg
      301 gcaagctgcg gaaaaacttt aagcatatct tggcggacaa gttcggtcat acgcgcggga
      361 tcgtcgataa attcatggtc tttaccgccg aaaagcaatc tgccgtccgc actgaggcgg
      421 taatagtcca aaatatggcg gttgtcgcat actgccatat tgttgcggat aagccctttt
      481 gcgcgcgccc ccaagggttc ggtggcaata ataaaggtgc tgacggcaat cgccttgcgt
      541 tccaaaggct taaatatcgg actcaaaccg gcataggtat tgacggcgta caccagattt
      601 ttgcattcga cgctgccttc gggcgtgtaa accagccaac cgttttgatg cggttcgatg
      661 cgcgtcatcg gggattgctc gaaaatctgc gcaccggctt cggcagcggc acgagcgatg
      721 cccaaagtgt aattgagcgg atgcaggtgt ccggataagg ggtcgaattg tgccccttgg
      781 tacatatcgc tgtcaagctg ctgtttcaac tcggctttat cccaaagttg ataatgactc
      841 gcaccgtaat gccgttgggc gtgttcatgc cactgctgca actcttccca atgctgcgga
      901 cggacggcaa ccgtggcata accgcgctgc caatcacaat cgacggcatg tttgcggacg
      961 cgttcgtcca ccagttcgac cgcctgcaaa gactgttgcc aaaaccattg cgcccgctcc
     1021 aaaccaacct gtttttcaat ttcctccata ccgcaggcgt agtcgctgat aacctgaccg
     1081 ccgctcctgc cggacgcgcc gaagccgata cgcgcggctt ccaacacaac cgtttcatgt
     1141 ccctgctccg ccaagggcaa tgcagtgcac aaaccactca atccgccgcc gatgatacag
     1201 gtatcggttt tcagacggca ttgaagtttc ggataaacag tatgaggatt aaccgaacta
     1261 aaataataag aaggcagata ttcttgaaaa tcaggacgaa tcattatgtt tactttatca
     1321 ggtgtatttt cagacggcat gatatggttt gccgtaccat gccgtctgaa cgggaaatta
     1381 ctttaagaaa acaggctgac ctgataatgc ggtcagcctg ccctgtcggt caatgcgctt
     1441 tctcagcagc caatttttct tggcggtagg cttctgccgc ttctcggtcg cgcttggttg
     1501 cctgcctcat catccaataa ttgacgatga tgaccaatgt tccgatgatg ccgattagga
     1561 tggtcgccaa gacattcatc tgaggatcaa gacccagctt gattttggag aaaatcacct
     1621 gcggcaatgt ggatgaaccg gggccggaga ggaatgaggt aattaccaaa tcatccaaag
     1681 acagggtaat gccgagcaga aagcctgaag cgatggcagg ggcaatcaaa ggcaaagtga
     1741 tgacaaaaaa gattttcagc ggacacgcgc ccaaatccat tgcggcttct tcgagcgatt
     1801 ggtcaagctc aaccagacgc gaacggataa caacggtaat gtacgccata cacagcgtcg
     1861 tatgtccgag gaagatggtg aaaaagccac gatcgaagta gagatgttgt aaccattcgc
     1921 tgccctgcaa aaatatctgt acctgaataa tcagcagcag catagacaga ccggtaatca
     1981 cgtcgggcat caccataggt gcggaaatca tgccagcgaa caaggtactg ccgcgaaaac
     2041 gtttaatccg cgccatcgca tagcctgcca gcgtgcccaa aacgacggcg gcaagcgaag
     2101 acacaacggc aatccgcagc gacagccaag cggcttccaa gatggtgtcg ttttccagca
     2161 atgcgccgta ccacttggtc gaaaagccgc cccaaacggt taccagcttg gattcgttaa
     2221 acgaatagat gaccaaaaca accagcggga tatacagaaa cgccagcgac agtgccaaca
     2281 tcagtttcaa gaaccaagat aatttggatt tctgcattat ttggctcctt cttccaattc
     2341 gcggttttca taatgctgaa acagggcaat cggcacgacc agcagcgcga ccatcacgac
     2401 ggcgacggcg gaagccagcg gccagttgtt ttgatcgaag aacgcctgcc acaagacttt
     2461 accaatcatc aggttttccg aaccgccgac cagctcggga atgacgaact cgccgacagc
     2521 agggacgaaa accagcatgg agcctgcaat aatgccggtt ttcgacaaag gcagggtaat
     2581 cgtcaagaac gatttgaccg gccccgcgcc caaatcggaa gccgcttcaa gcaggcggtt
     2641 gtcgagtttc accagttgcg tgtatagcgg cagaatcata aacggcagat aggcgtaaac
     2701 catcaccaaa ttgagcgaaa aggcattgta gaacaaatcc aaaggctcgc tgataatacc
     2761 cattttaatc aacaggttgt ttacaatgcc gttatgcccg agcagaccca tccacgcata
     2821 gacgcgcaac aggaacgatg tccaaaaggg cagcataatg gcaagcagca aaccattgcg
     2881 gacagaagga ttggcacgag aaatcgcata ggcggtcgga taaccgacca acagacaaat
     2941 taccgtcgta gtcagcgcag tcttaattga agaccaataa gtcatcagat agatattgct
     3001 gttttcaccg tcgccgaacg gattgagcgt actccaaaaa ttttggaaga tgtctgcata
     3061 attttggtag ctgacagcaa tattcagacg acccaaatcc tcatctatcg tcgttaaagg
     3121 agtaaacggc gggatggcga tttcttgttc ggcaaagctg attttcagca cgatggcgaa
     3181 cggaatcaga aacagcacca aaagccaaat atacggtacg gcaatcaccg cacgctgccc
     3241 cggacggcgg aacagtttgt ttttcagttt attaaggttc attgcattcc ccttaaatca
     3301 acggaacaac ggagtcggtt ggttttccgg ccagctgata tagacggttt cgtcccaagt
     3361 cggcggtgta atgttgcgca cataccagta aggggcgggg acttggcttt tgacgacgcg
     3421 cccgttgccg agcttgatat ggtaaatggc gaagctgccc aaataggcga tttcttttac
     3481 cgtgcctttc gcccagttgt agtcgcccaa atattcgggt ttttctttat ataaatcaat
     3541 atcctctggt cgaatactaa cccaaaggtc ctgctcgctc ggaccaccca aaccgtgatc
     3601 gatgcggacg tggttttcca aaccttcgca ttcgataacg gcatagtcgg catgatcttc
     3661 aatcaccaca ccgtcaaaga tgttggtttc gccgataaac tcggcagtga agcggctgtt
     3721 gggatagtcg tacacgtcgc tgggtgtgcc gacttgctgc aactgaccgt cagacataat
     3781 ggcgatgcgg gtcgccatcg tcatcgcctc ttcttggtcg tgcgtaacca taatacaggt
     3841 tacgccgact tgttccagcg tattgaccaa ctcaagctgg gtttgttggc gcagtttttt
     3901 gtccaatgca ccgaggggct catccagcag tagaattttc ggacgttttg ccagactgcg
     3961 tgccaaagca atgcgctgct gctgaccgcc ggacaattgg tgcggtttgc gtttagcaaa
     4021 tttggtcatc tgaaccaggc ggagcatttc ttcgacgcgc gcggcgattt cgcctttagg
     4081 cattttgtcc tgtttcagac cgaaggcaat gttttgttct acggtcatat gcggaaaaag
     4141 cgcgtaactt tggaacatca tattgatggg gcgatcatag ggtgcaagtt tggtaatatc
     4201 ctgaccatca aggataattt ttccctgatt gggactttcc atacccgcca gcatacgcag
     4261 cagtgtagat tttccgctgc cggaactgcc caaaagggcg aagatttcgt gttgataaat
     4321 gtccaagtcg atgttatcga cagcgtaatt gtcaccaaac tttttcacca aaccttggat
     4381 tttgagataa ggtttggctg aagacgcagt ggttgcggtc ataatggcaa tactccaata
     4441 aaaagacgag taccggcaaa acggattttc gaatgggtga taaaaagctg tttgattgct
     4501 ggcgggagtt aaacgtttga tgccgtctga aactcttgta aagcgcacgg gcagcatgaa
     4561 atggaacaag attccaaaga actttatatt atattagttt atgcggtttt cgggcaatat
     4621 agtggatttg agcttgtcgg aaagcaacag aaaagaaaac accgcccatt tttctgggcg
     4681 gtgtcggaaa gcgtaattat ttacgcagac ccaagcgggt aatcaacgcg cgatacgtat
     4741 cgggctgggt acggcgcaag taggccagca ggcggcggcg ttggctgacc attttcaaca
     4801 ggccgcgacg gctgtggtgg tctttggggt tggctttgaa gtggggggtc aggtcgttga
     4861 tgcggaaagt caacagagcg acttgtactt cggaagagcc ggtgtcgcct tctttgcgtt
     4921 ggaaatcttt aacgatttgt gctttttgtt ctacggtcag tgccataatg aaaactccaa
     4981 aaatataaga atccccatag ggattcagac aagtttgcca agcctgaaga caacggcaaa
     5041 ctccctatgc ccaagatagg acaacgtggc attatgacac aattcccgca tttctgcaca
     5101 atattttaag accgtggaag taaccgttaa aaatgccgtc tgaagcattg tctgcttgag
     5161 acggcaatgt tcagacggca tatgcgcttc aaaccacgat ttcttctttc ttcttcggtt
     5221 ctttaccgat attgtcgcgg ctcaaaccga acattagcag aagcgggctg gcaaccaata
     5281 cggaagaata aatgccgaac acgatgccaa tggtcaacgc catagaaaag ccgtgcaagg
     5341 ccgcaccgcc gaacaccagc atggatacga ccatcgcctc ggtcgaaccg tgggtaatga
     5401 tggtgcggct catcgttgcg gtaatcgcgt tgtcgatgac ttccggcacg gcatgtccgc
     5461 gcatcgccgg cttgcggaag ttttcacgga tacggtcgaa gacgacgacg gattcgttca
     5521 cagaatagcc caatacggca aggatacccg ccaagacggt cagcgaaaat tcccattgga
     5581 agaaggcaaa gcagccgaga ataatcacga tgtcgtgcat attggcgata atggcagata
     5641 cggcaaaacg ccattcaaaa cgcatcgaca ggtaaataat gatgccgata acgacaaaac
     5701 ctaaagccat caatccatta cttaccaatt cctcaccgac ttgcgggccg ataaattcga
     5761 cttggcgcaa ggtaacgtcg ggactgtctt ttttcagcaa atccataacc tgattggaca
     5821 actgtgcgga agtaacacct tctttgttcg gcaggcggat catgatgtgt ttgttcgtac
     5881 ccaatgcctg aacctgtaca tcacctattt tcagcgtatc gaggcgttcg cgcatcttat
     5941 tgacatccgc accctgctga tattggactt ccattaccgt accgccggta aattcgacag
     6001 agaaattcag acctctggta accaaaaaga acacggcagc gataaacgta accaacgaaa
     6061 tgaaggtcgt cagtttgccg tagctcataa acggaatatc gcgtttgatt ttaaagagtt
     6121 ccatagctta ctccttacct gccgcagttt cggcttcagg tttccatacc gcaccgatgg
     6181 aaatattctg caatttgcgt ctgcgtccgt accacagatt gaccaacgca cggaatacga
     6241 cgacggagga atacatcgaa gtcagaatac ccaaacagtg tacgaccgca aaaccgcgta
     6301 ccgggccgga accgaatacc aaaagcgcga taccggcaat cagcgaagtc aggttggaat
     6361 cgacaatggt cgcccatgcg tgttggaaac cgagattgat tgcctgctgc ggcggcacgc
     6421 cggcacgcaa ttcttcgcgg atacgttcgt taatcaagac gttggagtcg attgccatac
     6481 ccaaagtcaa cgccagcgcg gccatacccg gtaacgtcaa cgttgcctgc atggcagaca
     6541 aaataccgat taggaacagt atgttggcac tcaatgcaat ggtagaaaag aaacccatca
     6601 gacgatagta aaccaccatg aatgcagcaa cgatggcaaa accccataaa gtcgaatgga
     6661 agcctttttc gatgttctcc ttacccaaag acggaccgat ggtacgttct tcgacaatct
     6721 gcatcggcgc ggcaagagaa ccggcacgca acagcaaaga cgtatcattg gcttcggctg
     6781 tcgtcatgct tccggaaatt tccacgcgtc cgccggtaat ggcagtacgg ataaccggcg
     6841 cggttacaac ctcggatttt ccttggtcga tcaaaaccat cgccatgcgt ttgccgacat
     6901 ttgcggcagt cagttcgcca aaaatgctgc cacccgcgct gtccaagctc agactgacgg
     6961 caggtgcgcc catttggtcg aaactcggtt gcgcatcgtt gatgttgtcg cccgtcagct
     7021 cgacctgttt gctgattagc agagtttcgg gacgatctcc gccgcttgaa agcagctcat
     7081 aaccgctcgg cacgttgcct tccaatgcct cgcgcaactt ggcaggatcg tcctccacca
     7141 tacgcaattc caaagtcgcg gtacggccga tgatgtcttt tgccttggca gtatcctgaa
     7201 cgcccggaag ctgcacgacg atacggtcgg caccggactg ctggatgacg ggttcggcca
     7261 cgcccaactc gttcacacgg ttgtgcaggg tagtgatgtt ctgtttgacc gcatcggaac
     7321 gcactttatt gaccgcctct tccgaaagcg tcaagacgat attgctgccg tctgaattca
     7381 gcgttgcttc aggaaacagc ttgcgcaact gcggcagagc cttttgcaca tcacctgcat
     7441 cctgcaaagg gacggtcagg ctgtttccag cctgacgcac cgtgccgctg cggatttttt
     7501 cgcggcgcag ttcgcggcgg atgtcgcccg aataacgttc aaacgttttc tgcatcgccg
     7561 ctttcatatc gacctgcatg gtgaaatgca cgccgccgcg caagtccaaa cccaaaaaca
     7621 tcggattggc tttgattttc gccatccatt cggggctgtc cgccaacagg ttgagcgcgg
     7681 taatataccc ttcgcccaaa gtgttttcga tgacgtcgcg cgctttaagc tgcgtttcgg
     7741 tgtctttgaa acgcactttc agtgaattgt ccacaacaaa catcccgtcg gtctgaatac
     7801 ccgcgttttg cagcgcggca tccactttga attgagtctg ttcgttgatg atgatggctt
     7861 gtcggttggt cgatacctgc acggcgggtg tttcgccgaa taggttgggc agcgaataca
     7921 ctgcggcaac cgcaatcgtg aacacaatca gcagatattt ccataaagga taacggttca
     7981 tcattgttcc ttaatggttg gaaccccacc ctttcggtgg tgtcggaatc gggctatttc
     8041 agaagaggca aaaacccttc ccagccaggc aagaccggaa ggcggcatct tgaatatgcc
     8101 gccctgcgtg tcggaacatg gtcaagcctt cggttggaat tcaaaacaaa gtgccgcatt
     8161 cgggcttttc cgatgcggct tgtcggcaca aatcaatcga cttttgcaga aatcgcattg
     8221 cgttccactt cgacctcgat ttttgtaccc tgtccgatat ccacggtaaa aaactgttcg
     8281 ccgactctgg ttaccttacc cttgaaacct gccgccaaga ccactttgtc gccgactttc
     8341 aaggcggcaa gcattgcctg atgcgctttg aatttctttt gctgcggacg catgatcagg
     8401 aagtagaaca ccaccataat caacactaaa ggagcaaatt gtgcaacagc ttgattcata
     8461 atttatccgt tctttctaat atggttgaaa atcgagaggg gtatataata acataagacc
     8521 gtaaacaata tatcgggttt gccgtccgta ccgaccgtat accgcagcct gcccgtccac
     8581 aaacccatgt ccttgacccg cctaatcttg aaattctatg cactgttgcg cctttttttg
     8641 ggcaaaaacg cccgcaccgc atggatttcg catcccgcct gtgccgggca cgaacccggc
     8701 gcaaaccatc ccgattcgcc cgaccgcatc ctctgcatcg agcaggcatt gcgccgcgcc
     8761 ggtatttggc agcacctcca aaccatagag gcggaagaaa tcagcgatac gcgcctcgca
     8821 cttgtccact cgagcaaata tctgaaccgt ttggaatctt gccagcccca aaaaggcaag
     8881 attttccgcc tggatgacga cactgcaatc agcacaggct cgctgtctgc cgcacgcttt
     8941 gccgccggtt cggcagttca ggcagtcgac atggtcatga accgcaaagc atggcatgcc
     9001 ttttgcgccg cccgcccgcc cggacaccat gcgggcagcg gcaaagccgg cggattctgc
     9061 ctgctgaaca acgttgccgc cggcgtcatg catgccattg ccgaataccg cctgaaacgc
     9121 attgccgtca tcgatttcga tgtccactac ggcgacggta cggcagaaat attcaaagac
     9181 gatccgcgca tcctgttttt caacctgttt gaaaccgacc ttttcccctt ccccgaaaac
     9241 aacgatatgc ccgacggcgg caatatggtg cacctgccct tgccgccggg aacgggcagc
     9301 cgcacattcc gcgaagccgt ccgcagacag tggctgcccc ggcttgccgc attcaaaccc
     9361 gaactggtgc tgctgtcggc aggattcgac gcacaccgtc tagacgaatc gggcaggctc
     9421 aacctgcacg aggcggattt tgcctggctg acacacaaaa tcattcagac ggcatcgggc
     9481 tgccccggca aaatcgtatc cgtactcgaa ggcggctaca cccttgaacc tctggcacaa
     9541 tctgctgccg agcatatccg cgtgctggca gggctgggca aatccgatgc cgcaaccgcc
     9601 tatcagaaaa cactggaccc aacaaaaaaa cggtttgcca aaccgaaaac cggacaggtg
     9661 cgccaaccta cccaatccga ccgttac
//
LOCUS       DAXHNE010000065         9680 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.065, whole genome shotgun sequence.
ACCESSION   DAXHNE010000065 DAXHNE010000000
VERSION     DAXHNE010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9680
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.065"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(156..701)
                     /locus_tag="ACFM4Z_001201"
     CDS             complement(156..701)
                     /locus_tag="ACFM4Z_001201"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC6021239.1"
                     /translation="MSKIICLTAGHSNTDPGAVNGSDREADLAQDMRNIVASILRNDY
                     GLTVRTDGEGKGNMPLRDAVKLIRGSDVAIEFHTNAAANKTATGIEALSTPKNKRWCQ
                     VLGKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAAKV"
     gene            complement(844..1365)
                     /locus_tag="ACFM4Z_001202"
     CDS             complement(844..1365)
                     /locus_tag="ACFM4Z_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021240.1"
                     /translation="MECHADVWHFILEKNMKKFYFVLLALGLAACGQEQSQKADAEQY
                     FFANKYQFADEKQAFYFERAARFRVLQQGLGGDFERFLKGEIPNQENLAKYRENITQA
                     VAYYADTNGDDDPYRVCKQAAQDAEILMKSMVTSGGGGTTDLDKESYQNYRKSMQECR
                     KTITEAEANLPKK"
     gene            complement(1367..1794)
                     /locus_tag="ACFM4Z_001203"
                     /pseudo
     CDS             complement(1367..1794)
                     /locus_tag="ACFM4Z_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
     gene            complement(1768..2526)
                     /locus_tag="ACFM4Z_001204"
                     /pseudo
     CDS             complement(1768..2526)
                     /locus_tag="ACFM4Z_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            2572..2829
                     /locus_tag="ACFM4Z_001205"
     CDS             2572..2829
                     /locus_tag="ACFM4Z_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFC6021241.1"
                     /translation="MKEFPDLEKHLIMLEVAVQDLEDRSLADSFVLSWLLQRITRQEP
                     TSIEQVRSFLQAQAKTFEPDSVQREHLESLLELVESAQELA"
     gene            complement(2896..4869)
                     /locus_tag="ACFM4Z_001206"
     CDS             complement(2896..4869)
                     /locus_tag="ACFM4Z_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFC6021242.1"
                     /translation="MEYMDLKSLLNFGLPNMPRTVIGLRKKAQRDGWQTRRRQGKGGG
                     VEYALPAEIRAAIMKRQSDELAEKMPKTLPQVRPGTAMSAQALAEAAKLLNEKQRSVA
                     DARCAVVAAVLGIKYQYDCSAKAAVAQFLGLLAEGKLDAVTLGNLEKANDRSRTAKVG
                     ERTLDGWISAYLKAENATERLVALAPKTTKAVKPIESYGWLPMFMQFHNIPSAPKLAH
                     SYRRFVQWAEAENMPVNDVPNLSMVRRVWDKLPLIMQERGRKTGAAYKSLLPYVKRDW
                     GALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAESCVAV
                     SDALRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIAGNPQGRGI
                     IERWWKDNLIEMARQYETFAGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQQKYLK
                     KLPSWSRFIADVVKCIDDYNNRPHGELPRHPDGGHYTPKAYREMRLEQDGIAPDMLSA
                     QELATMFMPQEVRKVQRGWLDLFNNSYFSVELAEYHKDEVRVSYDLSDASVVNVFDMD
                     GKFITKAQANGNTREAFPTARIDQLAENRRKGKIKRAENAIKLANAEVNPALEQAAVW
                     DELGHLGGNDIEAEYAVLPKTGTDDFVLFEADR"
     gene            complement(4871..5122)
                     /locus_tag="ACFM4Z_001207"
     CDS             complement(4871..5122)
                     /locus_tag="ACFM4Z_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6021243.1"
                     /translation="MQANEIYKSLEEKNISARMLASALGVTNQSVSEVIRNGRGSKRI
                     AEAIAKVIQKDLVEVFPHYKKTDCRDKKIAELKEMLGVG"
     gene            5253..6068
                     /locus_tag="ACFM4Z_001208"
     CDS             5253..6068
                     /locus_tag="ACFM4Z_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HFC6021244.1"
                     /translation="MERIDLALNLVKERSRLGYSRKNFAEQTGCSAESLRLYESGQVN
                     IPADFLLAAVQLGVDIQFVFTGIHSANLNAVSDDLNKKLDDAIGKSNVENQINGNVSN
                     AVIASSGSVVHQINTQNHVIRTRVDSKPGKEHITLEQASKLQQLVKQVAAAEEIAKRS
                     PKSIRAIWASLNAHCKVPSYKLIALSDYDKAETYLRKWLGRLSNTATSKNNDPDWRKK
                     KYAYIKLNVKQLELEDWLKSYLEKNFSVESLTELSDEDLQKTYAAVSTKKRKK"
     gene            6273..6443
                     /locus_tag="ACFM4Z_001209"
     CDS             6273..6443
                     /locus_tag="ACFM4Z_001209"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6021245.1"
                     /translation="MRIGNDLFVKRVQRIPGKLLVTSENPRYAPFEIDLSNTQDDIAI
                     IGRVEWYGRSID"
     gene            6653..7243
                     /locus_tag="ACFM4Z_001210"
                     /pseudo
     CDS             6653..7243
                     /locus_tag="ACFM4Z_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            7348..8016
                     /gene="hda"
                     /locus_tag="ACFM4Z_001211"
     CDS             7348..8016
                     /gene="hda"
                     /locus_tag="ACFM4Z_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HFC6021246.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            8013..8681
                     /locus_tag="ACFM4Z_001212"
     CDS             8013..8681
                     /locus_tag="ACFM4Z_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6021247.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGITNIIGTQLESGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVDEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
BASE COUNT         2514 a         2672 c         1983 g         2511 t
ORIGIN      
        1 cacgcctgat ttacccaagc gcaggctcgg atgttgtttc aaagagccta cccgaacagg
       61 gcttggcgtg attttgattt caggtaacgg tggtacgcca aaatcgtttt tcaactttgc
      121 acaatgggca acacataaag caatcaaaga ctttttcata ccttcgccgc tcccaattcc
      181 atcgcaatcg cgtccgcaat cgcgcggcag atgccccatt tggtcgtctt aaacaaggcc
      241 aaatcagtgt cgttactgat aaaaaacggc tcaaacacaa tgccgcctgc ctgcgcataa
      301 gccaggcgcg aatgttgccc tgcgttatcc ggcttaaagc cgtcttcgcc gcgcagtttc
      361 cagccggttt tcttggcaac ggctttgccc agcacctgac accagcgttt atttttcggc
      421 gtggacaagg cttcgatgcc tgtcgccgtt ttgttcgccg ccgcattggt gtggaactca
      481 atcgccacat ccgagccgcg aatcagcttg accgcatcgc gcaagggcat attgcctttg
      541 ccttcgccgt cggtgcgtac ggtcaggccg taatcgttac gcaggattga agccacaatg
      601 ttgcgcatat cctgcgccaa gtccgcctca cggtcgctgc cgttgactgc gcccgggtcg
      661 gtgttactgt gtccggcagt cagacaaata attttgctca ttaaagcctc cctcaaaatc
      721 agattaaaat gcactttcag aggcttacat tttcaaacgg catggctttt gcagcgggcg
      781 aaacagtgtc agtaggcaac aaaaaagccg cctgttgttc agacggcctt agaatcgttt
      841 attttatttt ttcggcaaat tggcttcagc ttccgttatt gttttacggc attcttgcat
      901 tgattttcgg taattttgat aactttcctt atctaaatca gttgtaccgc ctccaccgct
      961 tgttaccata ctcttcatca ggatttctgc atcttgcgca gcctgtttgc agacgcggta
     1021 tgggtcatca tctccattcg tgtccgcata ataagcgact gcttgagtaa tattttcacg
     1081 atactttgca agattttctt gattaggtat ttctcctttt aaaaacctct caaaatcccc
     1141 gccaaggcct tgttgcaata cacggaaacg ggcggcgcgt tcaaaataaa aagcctgttt
     1201 ctcatctgca aattgatatt tattggcaaa aaaatactgc tccgcatcag ctttctgcga
     1261 ttgttcttgc ccacacgctg ccaaacccaa cgccagcagc acaaaataaa actttttcat
     1321 atttttctcc aaaataaaat gccatacatc tgcatggcat tccattctaa aacaaattct
     1381 cctgctccgc ttccttatcc gcctgcttta aaattcgcca cacatggcgg tcgcttaggc
     1441 ggtgtgccaa ggccaaatcg ttaacggcct cataagcagg ggtaccgcct gccgtctgcc
     1501 ggtcaaactg actgcggatt ttacggtttc gcaactcata caaggccgtc tcgcaacggg
     1561 gtataaacag attgcacgga gcagccgacc ggccgcctca ctgccgataa tctcttccaa
     1621 gtatgcaaca cgggattgac tgtttttcgt ataaccctgc cgcaaaggat aagtcgtacc
     1681 gcccatcagg cgcaccagct ccagcgtttc attaaacccg atgaccgtaa tcaacgcttg
     1741 tacactatca gggagcagat gcttgaccgc atcaggcaaa gccgctttcg caatctcaaa
     1801 gagttcgtca tccggcaaag attcgcccaa attcagcgca acagacacgc ggctgtaaag
     1861 ctgtaccagc tcgccatgct taccgcgcaa attcgcaacc agtcggggca taccgctcaa
     1921 caccaagccg cagcccgtct catcgtgcag acggcgtaca atttcaaggg cgcgcaaggg
     1981 caggttttcc gcttcatcga ccacaatcag acggcccgaa tcgcgcaggc ggtcagatac
     2041 agactcaaac aaatcattca ggctgcccat cgccgatacc ttcgccgccg tcgccaactt
     2101 gcgcatcaag accaaagccg taaagctcgg attagcctca attaagatgg ctgcagggtt
     2161 tttctcgcag tagtttttga ccgcctgagt cttgcccaaa cccgcctgac cgtagatcac
     2221 cactatttcg ccgccttcgt gcgcatcgcg catcgcttcc gcaatacggc gtgtcgtctt
     2281 agtcgataca aatcccaaca ccagctcttc acgtcgcgcc ttactttcct gcatctccaa
     2341 aaacgcctcg attttcgtct cgatggtttc ataattgccg cctttatcgg cataagtgcc
     2401 gttcagatac atactgatgg atgccggcga agtatcgata ccgcgtgcca gttgggtttg
     2461 gttcatgcct gatttttctt taaattcaac cagtttttgt tgcaatgctt gattaatttg
     2521 tttcattttt aatatccttg agttttaaac aacctttaaa ggccgtctga aatgaaagag
     2581 tttcccgatt tagaaaaaca cctgatcatg ctggaagtcg ccgttcaaga cttggaagac
     2641 aggtcacttg ccgattcttt cgtactttcc tggctgctcc agcggattac ccgtcaagag
     2701 ccgacttcga ttgagcaggt tcgcagtttc cttcaagcgc aggcaaaaac gtttgagcct
     2761 gattccgttc agcgggaaca ccttgaatct ttgcttgagc tcgttgaatc cgcccaagag
     2821 ctcgcttgag tttcaatttt tcaacaagct cggccgaaaa cgctgcattt tgctgtttgt
     2881 ccatcatgtt ttcctttatc tatccgcctc aaacaacaca aaatcgtctg tgcccgtttt
     2941 cggcaatacc gcatactccg cctcgatgtc gtttccgccc aaatgtccca gctcgtccca
     3001 aactgccgcc tgttccaaag ccggattgac ttccgcattt gcgagcttga ttgcattttc
     3061 cgcccgcttg attttgcctt ttcggcggtt ttccgccagt tggtcgatac gagccgtcgg
     3121 gaaagcctcg cgggtattgc cgttggcctg cgccttagtg ataaacttgc cgtccatatc
     3181 aaacacattg accaccgacg catcgctcaa atcgtagctg acccgtacct cgtctttgtg
     3241 atactccgcc agctcgactg aaaaataaga gttgttgaac aaatccagcc aaccgcgctg
     3301 tacctttcgc acctcttgcg gcataaacat cgtcgccagc tcttgcgccg acaacatatc
     3361 cggcgcgata ccgtcctgtt ccagcctcat ttcccgataa gccttaggcg tataatgccc
     3421 gccgtccgga tgtcggggca gctcgccgtg cgggcggttg ttgtaatcat cgatacactt
     3481 gaccacatcc gcgataaaac gcgaccagct cggcagtttt ttcaaatatt tctgttgttc
     3541 ctccgtcaaa tccttgcctt tttccaaagc attaaaagca ctttccatct tgcggtacat
     3601 caggttcttg gtactgctgt ccatccccgc gcccgcaaac gtctcatact ggcgcgccat
     3661 ctcaatcaga ttgtctttcc accatcgctc gatgatgcct cgaccttgcg ggttgcccgc
     3721 gatacccgtt tcatgccgga tacccaatcg ggacgtaata cccgtgattt catggtctat
     3781 cgtcttgccg gtttggccgc cgccgttatc cgagtagtag atgatcggca aaccaaaatg
     3841 cttgaccccg atacgcagag cgtccgatac cgccacacaa ctttcagcga gagagaccga
     3901 aaaaccgacc acaaaccgcg tacaaccatc aataatcacc gtcacttccg gcttaaacgg
     3961 cctgccgtgt accggatgcg ccaccttcgc cttaaagctg tggccgtcgc cgatccaaac
     4021 atcgttaggc ttcaaagccc cccaatcacg tttcacataa ggcagcagcg atttataagc
     4081 cgcccccgtt ttcctgccgc gctcctgcat aatcaacggg agtttgtccc aaacgcgccg
     4141 caccatactc aagttaggca catcgttaac cggcatattt tccgcttcag cccactgcac
     4201 aaaccggcgg tagctgtgcg ccagctttgg cgcggacgga atattgtgaa actgcataaa
     4261 catcggcaac caaccgtagc tctcaatcgg cttaaccgcc ttcgtcgtct tcggagccaa
     4321 agcaaccaac cgctccgtcg cgttttccgc tttcaaataa gcagaaatcc agccgtctaa
     4381 agtacgttcg ccaaccttcg ccgtccggct gcggtcattg gccttttcca agttcccaag
     4441 cgtgaccgcg tccaatttac cttctgccag caagcccaaa aactgagcca ccgcagcctt
     4501 ggcagagcaa tcgtattggt atttaatccc caacaccgcc gccaccaccg cacatcgcgc
     4561 atccgccacc gaccgttgtt tctcgttcaa cagcttggcc gcttcagcca gtgcttgagc
     4621 cgacatcgcc gtccccggtc tgacttgggg cagggttttc ggcatcttct ccgccagctc
     4681 gtccgactgc cgtttcatga tggctgctcg gatttcggca gggagggcat attcaacacc
     4741 accaccctta ccttgacgcc ttctcgtttg ccaaccatcc ctttgcgctt tttttctcag
     4801 ccctattact gttcttggca tatttggcaa cccaaaatta agcaggcttt tgagatccat
     4861 atattccatt tcaacccact ccgagcatct ctttcaattc agctatcttt ttgtctcgac
     4921 aatctgtttt cttgtaatgc ggaaaaactt caactaaatc tttttgaatc actttcgcga
     4981 ttgcctccgc aattcttttg cttcctcgtc catttcttat gacctcagaa acagattggt
     5041 tagttacccc taaagccgaa gccaacattc ttgccgatat atttttttct tctaaggatt
     5101 tatagatttc gttcgcttgc ataatgcctc catgatagaa tccattactt acttacctta
     5161 caggctttaa tgcctgcctt aactgttacc tggatgatat acccaaaata tttggtatac
     5221 caaatatatt tggtactatt ttgcggtgtt ttatggaacg cattgattta gcattgaatt
     5281 tagttaaaga gagaagccga cttggatact ctcgaaagaa ttttgctgag caaactggat
     5341 gcagtgcaga aagtttgaga ctgtatgaaa gcgggcaggt caacattcct gcggactttt
     5401 tacttgctgc tgtccaatta ggggtggata ttcaatttgt ctttacaggc attcattcgg
     5461 caaatttaaa tgctgtttca gacgacctta ataaaaaatt ggacgacgct attggcaaat
     5521 caaacgttga gaatcagatt aacggcaacg tcagtaacgc tgttatcgcc tcatctggtt
     5581 cggttgttca tcaaattaat acccaaaatc acgtcatcag aacccgtgtc gattcaaaac
     5641 ccggcaagga acatataacg cttgagcaag cttcaaagct gcaacaactt gttaaacaag
     5701 ttgccgcagc agaagagatt gccaaacggt cgccaaaatc aatccgtgca atttgggcta
     5761 gtctgaatgc tcactgtaaa gttccaagtt acaaactaat cgcattgagc gattacgaca
     5821 aagcagaaac ttatctcaga aaatggctgg gtaggttgag caatacagca acatcaaaga
     5881 acaacgatcc cgactggcga aaaaagaaat acgcttatat caaattaaat gtaaaacagc
     5941 ttgagcttga agattggttg aaaagttacc tcgaaaaaaa tttctcagta gaaagcctga
     6001 ctgaattaag tgatgaagat ttgcaaaaaa catatgcagc tgtatcgact aaaaaacgga
     6061 aaaaataggc ttaaacatga aactccgcct ttaaattcaa gtttcatgtt tagcttatgg
     6121 tcttcagatg ctttttttag atgtatgata ttaaaagatt ttttcaaaca tggaactatt
     6181 atacaagttc catgttcagt ttctagttaa agccttttat aaacattcta atcaaccacg
     6241 ccgaaaccga gccgcgcgac ggcctatatg tcttgcgtat aggcaatgac ctctttgtca
     6301 aacgtgtgca acgtataccg ggcaaactgc ttgtaacgtc tgaaaatcca cgttacgcac
     6361 cgtttgaaat agatttgagt aatacccaag acgacatcgc catcataggc cgtgttgagt
     6421 ggtatggccg gagtattgat taaatccatt ttaaaaacct cttaaaacag ttttaaaaat
     6481 tccctaatct cgtcagcttt aaacagaaaa ccgcgcattc cggcgcggtt ttatgaaaaa
     6541 gctggcgtaa cttttccgga tacaaaaaaa cgccgaaatc cacgtcttcc gaagatttca
     6601 gcgttttttt actctatttg ttccttgtgc aaaaactaac agtcccccac aggaaaacgt
     6661 cgctctgttc cgccacaacg gcgaaatcct gttgagcggc atcaacatca aactcaacag
     6721 cggcgattcg ctgctgatac gcggcccgag cggttgcggc aaaacctcgc tgctgcgcgt
     6781 gctggctggg ctttggccgt tcggcagcaa cggcaaagtc agccgtccgc cgcatcaaga
     6841 catcctcttc ctgccgcaac gcccgtacac ggcacagggc agcctgcgcg acgcggtttg
     6901 ttatcccgac atcgacaaac agcatccgga gctaatcgaa gccatgaaca cctgccgctt
     6961 gggttatctg gttgataaat tagataaaac cgacgactgg caacacaaac tctccccggg
     7021 cgaactgcaa cgcgtcgcct tcgtccgcgc cctgctttcc aagcccaaaa tcgtcctgct
     7081 cgacgaagcc accgccgcct tggacgaacc ggccgaagcc gcgctctacc gcgccttaaa
     7141 gcaaaaactg cccgacagca tcatcatcag catcggacac cgaagcacgc tcaacgcgtt
     7201 ccacaatatg cgcctcgatg tcggcaacgt tgcctgcggt tgaacgatgc cgtctgaagc
     7261 cgttttcaga cggcatcgtt caaattggaa aggcgcgcga aaacaacgta caataaaccc
     7321 gtttaccacc cctcggaatg ccgaaccgtg aaccagctta ttttcgactt tgccgcacac
     7381 gactatccga gtttcgacaa attcctcggc acggaaaacg cggaactggt ctatgtcctc
     7441 cgacacaaac acggacagtt tatctatgtc tggggtgaag aaggtgcggg caaaagccat
     7501 cttttgcagg cgtgggtcgc acaggcgctc gaagccggca aaaacgccgc ctatatcgat
     7561 gccgcctcca tgccgctgac cgatgccgcg ttcgaggcgg aatacctcgc cgtcgatcaa
     7621 gtcgaaaagc tgggcaacga agaacaagcc ctgctttttt ccatcttcaa ccgcttccgc
     7681 aacagcggca aaggcttttt gctgctcggt tcggaataca cgccccagca gctcgtcatc
     7741 cgcgaagatt tgcggacgcg tatggcgtac tgcctcgttt acgaagtcaa gcccttaacc
     7801 gaccaagaaa aaatcgacgc gctcgccagt atggcggcgg cacgccaagt aaccgtcgat
     7861 tccgaaattt tcgaatacct gctcaaacac tggcggcgcg atatggacag cctgatgatg
     7921 atgctcgaca cgctggacaa ctacgccgtc acaatgggca aacgcatcac cctgccgctt
     7981 ttgcgccagc ttttgaaaca acaggaaacc caatgaaaaa cctcgccatc ttcgacctcg
     8041 acaacaccct catcaacacc gattccgacc acgcctggcc gcaatacctc atcaaaaaag
     8101 gcttggttga cgctgccgaa accgaagcgc aaaacgaaaa attctaccgc gactaccaaa
     8161 acggctgcct cgacatcgac gcattcctca aattccacct cgccccgctc gcccgttaca
     8221 gcaaagaaga gctggcggaa tttcaccgcg aatttatggc ggaatacatc atcccccaca
     8281 tctcgcctat gcagcgtatg ctggtgcaga gccaccaaat ggcaggcgac gaaaccctcg
     8341 tcatctcatc gaccaacgag ttcatcatca cccccgtctg ccgccttttc ggcattacca
     8401 acatcatcgg cacacaactc gaatccggtt ccgacggccg ctacaccggc aattacatcg
     8461 gcacgcccag cctcaaagaa ggtaaaatca cccgcctgaa ccaatggctc gccgaacgcg
     8521 gcgaaacgct cgaaagctac ggcaaaacct atttttacag cgactccaaa aacgacctgc
     8581 cgctgctgcg cctcgtcgac gaacccgtcg ccgtcaaccc cgatgccgag ctggaaaaag
     8641 aagccaaaga aaaaggctgg ccggttttga atttcaaatg acgcaatgcc gtctgaagcc
     8701 cgattcggcg ttcagacggc attgaggctg aaatccaaaa aactcaaagc cacaggaatt
     8761 tatccggaaa aacaaaaaac acaggaattc atcgggaaaa acaacaatct ttccaccgtc
     8821 attcccacga aagtgggaat cttgatccgt ccgcacggaa acttatattt cgtcatttgc
     8881 acccgacata attcaatact ttattttgca ataaattaac ggatgctgac aaattagatt
     8941 ggattaatta ccctattatc catattgggt tatcgggttg ctaattgagc agagcgtagc
     9001 aaaccataaa gtgagtgttt ccacccactc tgcatttact atgaagtggt tattaaatat
     9061 gataatgaga cctattaaat ttagtatggt aaatatgtta ttatttattg ttatatgtaa
     9121 ttcatttctt gatctgctcg ttcaattatg tacaatttta tttcatagcc aaaaaatata
     9181 ctttattaca ttatttttat tatttatttt taattttgct acaaaatcta tctatatggc
     9241 aattatttat cctatttcat atttttttat cgtaaaaaat attatcctta ctctaggaaa
     9301 gtgataattc tattatcatt agcattatct atatatttta cttttatgga cttttacttt
     9361 ttttccatat attcagataa ccttagctat gaaacggagc ctttacattt atacatccct
     9421 attattatta attttttctc acttttagtt tctaatttta ttttatcttt tatcaacaag
     9481 taaaggttca gttatggaaa tttttgaggt aaatgtatat acaaaataca atccaaaaga
     9541 tcctaagcaa accaccaatc ccacaccacc cttaccggtt caaatccgca caaaaaccca
     9601 catctcgtca ttcccacgaa agtgggaatc taggacgtat agtggattaa caaaaatcag
     9661 gacaaggcga cgaagccgca
//
LOCUS       DAXHNE010000066         9630 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.066, whole genome shotgun sequence.
ACCESSION   DAXHNE010000066 DAXHNE010000000
VERSION     DAXHNE010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9630
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.066"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..202
                     /locus_tag="ACFM4Z_001213"
     CDS             <1..202
                     /locus_tag="ACFM4Z_001213"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021248.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
     gene            370..849
                     /locus_tag="ACFM4Z_001214"
     CDS             370..849
                     /locus_tag="ACFM4Z_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021249.1"
                     /translation="MEKIIDSKVRETNFNQAWRVIRAMQIDNISDEQVVIVRMSCYLK
                     KDEIFKNIERYGYQSSDIKKISSPFPLNILPAPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDKDKLFKSGETLYSNFKSIKNQCKNYFFAVLILMPLTLKAV"
     gene            938..1129
                     /locus_tag="ACFM4Z_001215"
     CDS             938..1129
                     /locus_tag="ACFM4Z_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021250.1"
                     /translation="MLPTRSVSSLLLGEYLEFEENGTKIAAEIDSAWHFLGAACRISI
                     NGKYYAGNRIVWFAKKAES"
     gene            1187..1843
                     /locus_tag="ACFM4Z_001216"
     CDS             1187..1843
                     /locus_tag="ACFM4Z_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC6021251.1"
                     /translation="MMKFKYVFLLACVVVSLSYRLNAAPMFNDNPVVYGKIKVQSWKA
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVLEKLDKEQMRASFEDMRRIMP
                     DLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVLRGIDGNTVLLADPSLGHVSM
                     SRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFTHHPKRQTEFAVGQIRQARAE
                     "
     gene            1970..3265
                     /gene="hisS"
                     /locus_tag="ACFM4Z_001217"
     CDS             1970..3265
                     /gene="hisS"
                     /locus_tag="ACFM4Z_001217"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFC6021252.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVNHWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIRDYLTLEINSLGKREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGERADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAEDLTHTLQQWKNA"
     gene            3266..3895
                     /locus_tag="ACFM4Z_001218"
     CDS             3266..3895
                     /locus_tag="ACFM4Z_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HFC6021253.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELTKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFS
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            4045..5502
                     /gene="der"
                     /locus_tag="ACFM4Z_001219"
     CDS             4045..5502
                     /gene="der"
                     /locus_tag="ACFM4Z_001219"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFC6021254.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEEIL
                     ENFPEPEVEEADAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     ERKGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            5533..6432
                     /locus_tag="ACFM4Z_001220"
     CDS             5533..6432
                     /locus_tag="ACFM4Z_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFC6021255.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAASLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            6484..6900
                     /locus_tag="ACFM4Z_001221"
     CDS             6484..6900
                     /locus_tag="ACFM4Z_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HFC6021256.1"
                     /translation="MLKHLAFLLPAMMFALPAASAVLTSYQEPGCTYDGGVGKDRLPS
                     GKGIWRCRDGRGYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            6966..7532
                     /gene="dcd"
                     /locus_tag="ACFM4Z_001222"
     CDS             6966..7532
                     /gene="dcd"
                     /locus_tag="ACFM4Z_001222"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFC6021257.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(7596..8300)
                     /locus_tag="ACFM4Z_001223"
     CDS             complement(7596..8300)
                     /locus_tag="ACFM4Z_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFC6021258.1"
                     /translation="MLRSILAASLLAVSFPAAAEALNYNIVEFSESAGVEAVQDTMSA
                     RLQVAAEGRDKNAVNAEFVKKFNKFIRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDELNRFIADVQADAALEYTDFHVSRERRNEVIDQVSKDAILRFK
                     ARADKLSGILGTSGYKIVKLNLGHIGSHIAGEGAAQAKMLRAMPMAASYGVEGTDSAA
                     PGVEEISISVNGTVQF"
     gene            8446..9630
                     /locus_tag="ACFM4Z_001224"
                     /pseudo
     CDS             8446..9630
                     /locus_tag="ACFM4Z_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678130.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         2611 a         2443 c         2305 g         2271 t
ORIGIN      
        1 aatgcaattg atatgtgccg attggacagg tataggaaat tttatgaaaa ccttcgaaaa
       61 aacatggtct gcacaatatc gggatatgga aatttcagta cggaattttt ggaatttgga
      121 gcggacaggg gcggaagtct atatcaacgg aaggcgggtt tatcataacg aagccgaaat
      181 ggcgtctgct tctttgcgtt agctaatggg ggaatacctg gaatttgaag aaagcggtac
      241 gaaaattacc gttgaaatcg gcagcgcgtg gcattttaat gaaactataa gaaatattaa
      301 tacacattta agaggtacga aacttccagg ttctaactgg agagttcaag ataaggggca
      361 ttttcatctt tggaaaagat aatagattct aaggtaaggg agactaattt taatcaggca
      421 tggcgtgtta tacgcgccat gcaaatagat aatatatccg atgaacaggt tgtgattgtc
      481 agaatgtctt gttatcttaa gaaagatgaa atttttaaga atatagaaag atatggatat
      541 cagtcatcag atattaagaa aattagttct ccttttccat taaatatttt acctgctccc
      601 aaaccttatt atttttatct ttatgatacc catttgaatc aaatttatga tttggcttgg
      661 tcatgtaatg attttttaat gtttgaaata tttattttgg atagggaaag catcaaatat
      721 atagataaag ataaactttt taaatctgga gaaacactat attcgaattt taaatctatt
      781 aaaaatcaat gcaaaaatta tttttttgcg gttttgattt tgatgcccct tactttaaaa
      841 gcggtgtgag tcaggtggca gattataatt ttgttcctga tgcattttct ttattcttta
      901 tttaatcaaa ttatttgaat aagcgtaggg tgggcacttg ctgcccacgc gttctgtttc
      961 aagtttatta ttgggtgaat atttggaatt tgaagaaaac ggtacaaaga ttgctgcgga
     1021 aatcgacagt gcatggcatt ttttgggcgc ggcttgtcgg atttcaataa atggaaagta
     1081 ttatgcgggt aatcggattg tttggtttgc caaaaaagcc gaaagctagg aataatttga
     1141 caagggcagc ctgaaagatt ttcaggctgc cctttatgtg gacaatatga tgaagttcaa
     1201 atatgttttt ctgttggcgt gtgttgtcgt ttctttatct tatcgtttga atgctgcacc
     1261 gatgtttaac gataatcctg ttgtttacgg aaaaatcaaa gtgcagagtt ggaaagcgcg
     1321 gcgggatttc aatattgtaa agcaggattt ggatttttcc tgcggggcgg cttcggtggc
     1381 gacgcttttg aacaattttt acgggcaaac gctgacggaa gaagaagtgt tggaaaaact
     1441 ggataaggag cagatgcgcg cgtcgtttga ggatatgcgg cgcattatgc ctgatttggg
     1501 ttttgaagcg aaaggctatg ccctgtcttt cgagcagctt gcacagttga aaatccccgt
     1561 catcgtgtat ctgaaatacc gcaaggatga tcatttctcg gtattgcgcg gtatagacgg
     1621 caatacggtt ttgcttgccg acccgtcgct ggggcatgtt tcaatgagca gggcgcagtt
     1681 tttggatgct tggcaaaccc gtgagggaaa tttggcaggt aagattttgg cggtcgtgcc
     1741 gaaaaaagcc gagacaattt caaataaatt gtttttcaca catcatccca agcggcagac
     1801 agagtttgca gtcggacaaa tcaggcaagc acgtgcagag taagtcaaat gccgtctgaa
     1861 aactgtttca gacggcattt ttacccgtcc gcaaaccacc cccaacctca caagccctta
     1921 aaaatccgct ataatcgccc gcaatttgat ttaagcattc ttacttgaaa tggcacaaaa
     1981 aatccaatcc gtcaaaggca tgaacgacct tctgcctgtc gagcaaaaag atttcaaact
     2041 gacggctgcg ttttggcagg cgtttgaaga tacggtaaac cattggacgc gcgcttacgg
     2101 ctatcagcaa atccgtacgc cgattgtcga gcaaaccggt ttgtttgtcc gctctatcgg
     2161 cgaggaaacc gatgtggtcg gcaaggaaat gtacaccttc tccgattcaa acgattcttt
     2221 gagtttgagc ttgcgtcctg aaggtacggc ttcttgtctg cgtgcggtgg tcgaacacaa
     2281 ccttctgtac aacagcccgc aaaagctgtg gtatatgggg ccgatgttcc gccgcgagcg
     2341 tccgcaaaag ggccgttatc gtcagttcca tcaggtcggt atcgaggctt tgggttttga
     2401 agggccggat attgatgcgg aaatcatcgc gatgtctgcc gacttatggg aaaaattggg
     2461 tatccgcgat tacctgactt tggaaatcaa cagcttgggc aagcgtgagg aacgcgcggc
     2521 acaccgtgcg gcattggttg aatatctgac ccgttatgaa gataaattgg atgaagacag
     2581 caaacgccgt ctgaaaacca atcctttgcg cgttttagat acgaaaaacc ctgatttgca
     2641 ggaaatctgc aacgccgcgc cgcgtttggt ggattatttg ggcgaggctt cgcaaaacca
     2701 ctatgcacgc ttcaaagcga tgttggacgg tttgggtatc caatatattg aaaatccgcg
     2761 cttggttcgc ggtttggatt attacaatca aacggttttt gagtggacga ccgacaaact
     2821 cggcgcgcag gcaactgtgt gcggcggcgg ccgttacgat ggtttgattg aggaacttgg
     2881 cggcaaacct gcgccgtcca tcggctttgc gatgggcatc gaacggctgc tgcttttggt
     2941 gagcgaatac ggctcgctgg aagtgaacgc tgcgcctgat gtctatgcaa tgcaccaagg
     3001 cgaaagggcg gacttgcagg tgatgaaata cgcgcaagcc ttgcgcgcgc aaggtttcaa
     3061 tgtaatgcag cattccggct atcaaagcct gaaagcgcaa atgaaaaaag ccgacaacag
     3121 cggcgcgcgc tttgccctga ttgtcgcgca agacgaactg gcgaacggta cggttacgct
     3181 caaagacatg aacggcgcac acggtcagca aactgtcgcc gccgaggatt taacccatac
     3241 tttacaacaa tggaagaacg cataaatggc agcccatctc gaagaacaac aagagttaga
     3301 caactttaaa tatttttgga aaaccacggg caaatggctg tttgccctgc tgattttggc
     3361 agcactcggc tacttgggat acacggttta ccaaaaccgt gcggcttccc aaaatcagga
     3421 agcggcggcg gtgctggcaa acatcgtgga aaaggcgcaa aacaaagccc cgcaaagcga
     3481 aattaatgcc gaattgacca aactccaaca aagctacccg cattctattt ccgccgccca
     3541 agccacgctg atggcggcgg caaccgaatt tgacgcgcag cgttacgatg ttgccgaagg
     3601 ccatttgaaa tgggtgttgt ccaaccaaaa agacagcctg attcaagcgt tggcggcgca
     3661 gcgtctgggc gttgtgttgt tgcaacaaaa aaaatacgat gccgcgcttg ccgcgctcga
     3721 cacgccggtt gaagcggact tctcccccct gctgatggaa accaaaggcg atgtctatgc
     3781 cgcacaggga aaaagccagg aagccttaaa aaactacggt caggctttag aaaaaatgcc
     3841 tcaagattct gtcggtcgtg aattggttca aatgaaactc gattcgctga aataaatgcc
     3901 gtctgaaatg ccaccctctc ttcagacggc atttcagaca acactatcct acggcaattc
     3961 ccgtaaaacc atctgccgtg tcccaacgga cacctgctcg tgttcagttc tcccgaacgg
     4021 cacttgacac agaaagactc aatcatgaaa ccaaccatcg cgcttgtcgg ccgccccaac
     4081 gtcggcaaat ctactttatt caaccgtttg acgcgcacca aagacgcgct cgtgcatgac
     4141 ctgcccggcc tgacgcgcga ccgccattac ggacacggca aagtcggcag caaaccttat
     4201 ttggtcatcg ataccggagg ttttgagccg gttgtggata gcggcatttt gcacgaaatg
     4261 gcaaaacaaa ccttgcaggc tgtcgatgaa gccgatgccg tcgtattttt ggtggacggc
     4321 cgcaccggtc tgaccccgca agacaaaatc attgccgacc gtttgcgcca aagcccgcgc
     4381 cctgtttatc tggcggtaaa caaaggcgaa ggcggcaaca gggccgtgct tgccgccgag
     4441 ttctacgaac ttgctttggg cgacccttat gttatttccg gtgcgcacgg cgacggcgtg
     4501 tattacctga ttgaagaaat tttagaaaac ttccccgagc cggaagtcga agaagccgat
     4561 gcaaaacatc ccgtttttgc cgtcatcggc cgtccaaacg tcggcaaatc tacgctggtt
     4621 aacgccattc tcggcgaaga gcgtgtgatt gcattcgata tggcaggtac gacacgcgac
     4681 agcatccaca ttgattttga acgtaaaggc aaaccgttta ccatcatcga taccgcaggc
     4741 gtgcgccgtc gcggcaaagt cgatgaagcg gtggaaaagt tctccgttat caaagccatg
     4801 caggcggttg aagcggcaaa cgtcgctgtt ttggtattgg acgcgcagca ggacatcgcc
     4861 gaccaagatg cgacgattgc aggtttcgct ttggaggcag gccgtgcttt ggtggtggcg
     4921 gtcaataaat gggacggcat cagcgaagaa cgtcgcgagc aagtgaaacg cgatatcaac
     4981 cgcaaactgt atttccttga ttttgctaaa ttccacttta tttccgcatt gaaagaacgc
     5041 ggcatagacg gattgttcga cagcattcag gctgcctaca acgcggcgat gattaagatg
     5101 ccaacaccga aaatcacgcg cgtattacaa agcgcgatcg agcgtcagca accgccgcgt
     5161 gccggcttgg tgcgtccgaa aatgcgttat gcccaccaag gcggcatgaa ccctcccgta
     5221 attgtggtac acggcaattc gctgcacgcg atttccgaca gctatacgcg ctatctgacc
     5281 caaacgttcc gcaaagcctt caatctgcaa ggcacgccgc tcagaattca atacaatgtt
     5341 tcggaaaacc cgtatgaaaa tgcggaagac aaaccgaaga aaaaaccgct gcgccgcgtc
     5401 agcctgagca accgtattga gaaacgcgaa ggccgaaagg aagagaaaaa ccgcttcaag
     5461 aagaaaacca aagtcagtgt aaaaaaacaa ttcagcaaat aattcccgac atttcaaaca
     5521 ataggaaaca ttatgtggtt caagcagatt agtttttatc cgctcaacaa agaaaagctg
     5581 cctgaggcag acgtacttgc cgacaaactt gctgaagctg aatttaccca ttgccaaggc
     5641 ttagactggt tcagcgaagg ctttaccgca ccggtttcgt tctctcccga actcgttttc
     5701 cctgccgact ttaccttgcg cgtcgccctg aaaaaagaag aaaaagtcct gcctgccggc
     5761 gtcatccgcg atattttgga agagaaggta gcggaaatcc aaaacaatga agcccgcaat
     5821 gtcggtcgca aagaaaaaca agagcttaaa gagcaaatta cagacgacct gctgccccga
     5881 gcgtttaccc gcagcagccg tacagaagcg gtgtttaaca cccgccacgg ctacctgctc
     5941 gtcaataacg cggcttccgc caaagcagaa aacatcctga ccaagctgcg cgaagctttg
     6001 ggcggtttgg aagcctcgct gccgaatacc aaacaatcgc cctcttctct gatgaccggc
     6061 tggctgttgc aagggcattg cgaaggcggt tttgaattgg acagcgattg cgaactcaaa
     6121 ggtacgggcg atattgttcc cgtcgtcaaa gtatccaaac aagatttaac cgccgacgaa
     6181 gtggttcaac acgtcaaaaa cggtaaaacc gtaacccagc tcggtttggt ttggcgcgaa
     6241 caaatcgcct ttatcctcac tcaagacttc acactcaagc gcatccaata cctcgacgta
     6301 ttgcaggaag aagccgaaag caacggcgac gatgccgcca gccttgcctt cgcctcgcaa
     6361 atcctgatgg ctgaatccgt cagcaccatg ttggaagagc tggtttccta tttgggcggc
     6421 tggcaagatt gatttttcag acggctttta taccgtcagc caaaatccaa tggaataacc
     6481 cttatgctta aacatctcgc attcctactg cccgccatga tgttcgccct ccccgccgcg
     6541 tccgccgttc tgacttccta tcaagaaccc ggctgcacct acgacggcgg agtcggaaaa
     6601 gacaggcttc cttcaggcaa aggcatatgg cgttgccggg acgggcgcgg ttataccggt
     6661 tcgtttaaaa atggcaaatt cgacggacaa ggcgtttata ccgttgccgc caaccgcgaa
     6721 atatttatcg aaccgttcaa ttccgacagt accaaattcc gcaacatggt actctcgggc
     6781 acgttcaaaa aaggcttggc acacggcaga tttaccgtct cgcaaaacgg cgaaaccctc
     6841 ttcattatga aatgcgaaaa cggcatgatt aaagaagtga agctgcccaa aaacaaataa
     6901 gccccgtttt cagacggcat cccccaatgc tttgtccgaa cactgcaaga aacaaggaaa
     6961 acattatgag catcaagtcc gacaaatgga tacgccgcat gagcgaagaa ttcggcatga
     7021 tcgacccttt cgagccgaac caaatcaaag aagccgacgg caaacgcatc atctcctacg
     7081 gtacgtccag ctacggctac gacatccgct gcgccaacga atttaaaatc ttcaccaaca
     7141 tcaacagcac catcgtcgat cccaaaaact tcgacccaaa aaacttcgtt accgttgaag
     7201 acgactgctg catcatcccg cctaattcct tcgcactggc gcgcacggtc gaatatttcc
     7261 gcatcccgcg caacgtcctg accgtctgct tgggcaaatc cacctacgcc cgctgcggca
     7321 tcatcgtcaa cgttaccccg ttcgaaccgg aatgggaagg ctacgttacc ctcgaatttt
     7381 ccaacaccac ccccctgccc gccaaaatct acgccggcga aggcgtggcg caagtcctct
     7441 tcttcgagag cgacgaaatc tgcgaaacct catacaaaga ccgcaacggc aaatacatgg
     7501 gacaaaccgg cgtaaccctg cccaaagcct gaaatataaa tgccgtctga acccttatcg
     7561 tcgggtttca gacggcattt gccgttcacc tcaaatcaga actgaaccgt cccattgacg
     7621 ctgatgctga tttcctccac acccggcgcg gcggaatccg taccctccac gccgtaactt
     7681 gccgccatcg gcatggcacg aagcattttt gcctgagcag ctccttcacc cgcgatatgg
     7741 ctgccgatgt gtcccaaatt taatttgacg attttataac cggacgtacc caatatgccc
     7801 gacaacttgt ctgcacgcgc cttgaaacgc aaaatagcat ctttactgac ctgatcgatg
     7861 acctcgtttc ggcgttcgcg cgacacatgg aaatccgtat attccaatgc ggcatctgcc
     7921 tgaacatcgg caataaaacg gtttaactca tcaaaatccc tcccttcgac cttaaattcc
     7981 gcacgctcct cccaacctgt ttgaatgcgt ctgccgttgg tatattgata gcgcggcatc
     8041 gcactgcgcg ataccaattc ggttttaaag ctaccatttt tcgattttct gatgaacttg
     8101 ttgaattttt taacaaactc ggcattgacg gcatttttgt cccgtccttc cgccgccacc
     8161 tgtaaacgcg cggacattgt atcctgaacc gcctcgacac cggccgattc ggaaaattcg
     8221 acaatattgt aattcaatgc ttcagccgct gccggaaaag ataccgccaa tagggaagcc
     8281 gccaaaatag aacgcagcat atccgtatcc ttacttataa aaccgccaat ctagcatatc
     8341 ggcgcgctaa attgcgttag cgtaaggcaa caatttaaat attcggtctg cgtctgtctt
     8401 ataataaccg cctttccgat acggaatccc actatgccca aacccttgca cgtcctcgtc
     8461 atcccctcat ggtatccgca atccgaacag gatgtggacg ggattttttt caaaatcagg
     8521 cactggcatt gcagaggaaa ggcatcaaaa ccgccgtgct tgcaccgatg ttccgctact
     8581 tgcggaaaga aacagcaagc atcctgaccg gccttacggt tttgcaaaat atcggcaaag
     8641 cggtttggac atctatgcat ggcacggcat atatttcttc ccccgctttc cgttcatcga
     8701 catcgactgc atccgctggg tgtgtgcggg tttgaaagct ttcaaacact acatccgcga
     8761 aaacgggctg cccgacctga ttcacgccca ccgcatgaac tatgccggca tactcgccca
     8821 aaaaatttcc caaaaatacg gcatccccta tgtcgtcacg aaacacagca gcaccatcac
     8881 gcgcggtctg atccgtcccc accaatggca gccgatggaa aaagcagcgg cgcacgcctc
     8941 cgcactgctc gctgtcagcc gccatttcgc acacgtcctg caacacaaat acggctgcga
     9001 atggcagtac ctcccccaac atactgggcg gaatattcaa ccgaccgttt gaacaaagag
     9061 aaaaaaacaa caaaccgcat ttcgtgttct gcaccgtctc gcaccttcgc cgtctcaaag
     9121 gacacgatgt cctactcact gcctttgccc gggcgttggc acaatgcccg caactgcgcc
     9181 tgaacatcgg cggcagcgga caggaagaac agcggctgaa acagcaggcg gcagacttgg
     9241 gcattaccca tgccgttaca tttttgggcg cattgcagcc cgaagcagtc ttggatttga
     9301 tgaggaacag cgacgcattc gtccttgcca gccgcaccga aaccttcggc gtggtctata
     9361 tcgaagcact gtcccaagga ttgcccgtca ttgcgacacg ctgcggcggt gcggaatcta
     9421 ttgtttcaga cggcaacgga tatttggttc ctgttgacga cgacgatgcc cttgccgacg
     9481 cactcatcaa aatgtatgaa caccactctg attttgaacc tgcccgactt agggaaaact
     9541 gtctgaacga atttggcgaa aatgccgtta taggcaggtt gatcggcatt ttccgacagg
     9601 caatcgcaga atacggtaag aaaataccgg
//
LOCUS       DAXHNE010000067         9550 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.067, whole genome shotgun sequence.
ACCESSION   DAXHNE010000067 DAXHNE010000000
VERSION     DAXHNE010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9550
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.067"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..184
                     /locus_tag="ACFM4Z_001225"
     CDS             <1..184
                     /locus_tag="ACFM4Z_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HFC6021259.1"
                     /translation="VLGDAAEVVYKCTDYYNPKAEHSLIWNDPTVGIDWPLQGEPNLS
                     PKDLAGKVLAEAVTFG"
     gene            240..1361
                     /locus_tag="ACFM4Z_001226"
     CDS             240..1361
                     /locus_tag="ACFM4Z_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stealth family protein"
                     /protein_id="HFC6021260.1"
                     /translation="MNLNKIRKFFRESHIFFRDFLNKRFPPYNVEQPIPETEEFTLIN
                     ADANLASLTENVLPNFPIDVVFTWVDNTDKAWQEQYYRTLQPIDQEDIGLYATDPARF
                     SNHNELFYSVQAVQKFMPWVRNIFIVTADQKPKWLDENIHSKIKLINHSQLIDAKYLP
                     TFNSHVIEANLYKIPDLSEHFIYFNDDVFVARPLMPNHFFENNGLASLFVANKSFQKM
                     RQRGLITPTLTASEHALKLLKRHYPTININTPLVHTYVPLRKTSFQKAWSLFEDEINS
                     FLNNKVRHNSELNMASFLIPWLMYLDGYATPKREICYYFNIRSSHAQTQYKKLLFEKE
                     HLHMPHSFCINDSSSNNADKNYALHFRNFMDTYFEIETE"
     gene            1406..3430
                     /locus_tag="ACFM4Z_001227"
     CDS             1406..3430
                     /locus_tag="ACFM4Z_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFC6021261.1"
                     /translation="MIFSTFGLFKQRLLLTKFLDKISTDKTALFWGKKHPKRRKLAHI
                     LRLSLLNLEDGFLRSLGLGVAGYPPYSIVYDDIGIYYDTTRPSRLEQLILAADTMPSE
                     TLAQARQAMDFILQHHLSKYNHAPELSDDHPLRSPSKPETVLIIDQTFGDMAIQYGGA
                     DASTFELMFQTALNENPQADIWVKTHPDVLCGKKQGYLTQLAQQHRVHLLAEDINPIS
                     LLQNVDKVYCVTSQMGFEALLCGKPLTTFGLPWYAGWGVSDDRHPEINRLVQTQRRAT
                     RNLLQLFAAAYLQYSRYLNPNTGEAGSLFDVIDYLATVKRKNDKLRGELYCVGMSLWK
                     RAVAKPFFNVPSCRLKFISSTQKLARVKLSDDARILAWGNGKEAIVRFAEQHHIPLLR
                     MEDGFIRSVGLGSNLVPPLSLVTDDMSIYFNAETPSRLEYILQNQNFDDQDFQTALKL
                     QKMLTENHISKYNVGSSDFTAPSTDKTVILVPGQVEDDASIRYGSPQIYRNLDLLRTV
                     RERNPNAYIIYKPHPDVVSGNRIGHISPEDAARYADQTAEQADILTCLQYADEIHTMT
                     SLTGFEALLRGKKVSCYGLPFYAGWGLTQDLLPIPRRSRRLELWQLIAGTLIHYPDYI
                     HPETHQAINAETAAQILIRQKNMQKNNNGLHRGCFAKKLGKIKQLYRSFK"
     gene            3567..4826
                     /locus_tag="ACFM4Z_001228"
     CDS             3567..4826
                     /locus_tag="ACFM4Z_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFC6021262.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNLRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKNTSDSSTPTT"
     gene            5196..6602
                     /locus_tag="ACFM4Z_001229"
     CDS             5196..6602
                     /locus_tag="ACFM4Z_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6021263.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYAEAAFVLG
                     NHLLQNGQPEQALSWLEAAAAQRHPKALFSLLQQREHNGTPTGQLLNDYAWLGEQGHS
                     EAQLILMRYHAQRNDPQSLYWAELAAARYAAPAYYHLARHHQRQGDVETAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEPAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAADKEQAEAQAKLAQYALTGELSERDPFQAARYAKAAAEKNHPEALKIMGD
                     LYRYGLGIKADNHIAQDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAEAQACAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            6820..8034
                     /gene="gltS"
                     /locus_tag="ACFM4Z_001230"
     CDS             6820..8034
                     /gene="gltS"
                     /locus_tag="ACFM4Z_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFC6021264.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     IAAIVLFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            8133..9149
                     /locus_tag="ACFM4Z_001231"
     CDS             8133..9149
                     /locus_tag="ACFM4Z_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC6021265.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSAALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            9160..9357
                     /locus_tag="ACFM4Z_001232"
     CDS             9160..9357
                     /locus_tag="ACFM4Z_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021266.1"
                     /translation="MTTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            9375..9524
                     /locus_tag="ACFM4Z_001233"
     CDS             9375..9524
                     /locus_tag="ACFM4Z_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021267.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVAYADVSTHRTIQKTS
                     PYVPS"
BASE COUNT         2553 a         2670 c         2107 g         2220 t
ORIGIN      
        1 cgttttgggc gacgcagcgg aagtcgtgta caaatgcacg gactattaca accccaaagc
       61 cgaacactcg ctgatttgga atgatccgac cgtcggcatc gactggccat tgcaaggcga
      121 gccgaacctg tcgcctaaag acttggcagg caaagtattg gccgaagcag taacgtttgg
      181 ataacttcaa aacataacag aatcaactgt ttctattttg tagtattaga aagtttcaaa
      241 tgaatttaaa taaaattaga aaatttttta gagagtccca tattttcttt agggattttc
      301 taaataaacg ctttcctcct tataatgtag aacagcctat tcctgaaact gaagaattca
      361 ctctgatcaa tgcagatgcc aatcttgcat cactcacaga aaacgttttg ccaaactttc
      421 ccatcgatgt tgtttttact tgggtcgaca ataccgataa agcatggcaa gaacagtatt
      481 accgtaccct gcagcccatc gatcaggaag atatcggcct gtatgcgacc gaccctgccc
      541 gcttttccaa ccataatgaa cttttttatt cagtacaggc cgtacaaaag tttatgccgt
      601 gggtccgcaa tatttttatt gtaacggcgg atcaaaaacc taaatggtta gatgaaaaca
      661 tccattctaa aattaaatta atcaatcatt cgcaacttat tgatgccaaa tacttaccaa
      721 cgttcaattc ccacgtcatt gaagcaaact tatataaaat tccagattta agtgaacatt
      781 ttatttattt caatgatgat gttttcgttg caagaccact gatgcctaac cacttttttg
      841 aaaataatgg tttggcttcc ctatttgttg ccaataaaag ttttcaaaaa atgcgtcaac
      901 ggggtctaat cacgccgaca ttgaccgcat cagaacacgc attaaagctc ttaaaaagac
      961 attatccgac aattaatatc aatacaccgt tagtacatac ttacgttccg ctacggaaaa
     1021 catcctttca aaaagcatgg tcattatttg aagatgaaat aaattctttc ctaaataaca
     1081 aggttcgcca taattctgaa cttaatatgg ccagtttctt gataccctgg ttgatgtacc
     1141 ttgacggata tgcaacaccg aaacgtgaaa tttgctacta tttcaatata cgcagttcac
     1201 acgcccagac tcaatataaa aaattattat ttgaaaaaga acatcttcat atgccccact
     1261 ccttctgcat taacgattca agcagtaata atgcggataa aaactatgcc cttcatttcc
     1321 gtaattttat ggacacttac tttgaaatag agacagaata aatttttaca ttaatgatac
     1381 ttaatagata acataataat ttaaaatgat tttttcaaca ttcggcctat ttaaacaacg
     1441 tctcttgcta accaaatttt tagacaaaat ttcaacggat aagaccgcat tattttgggg
     1501 caagaaacat cccaaacgta gaaaattagc acatatctta agattgtcct tgctcaatct
     1561 tgaagacggc tttttacgat cgctcggact gggtgtcgcc ggctatcctc cctattccat
     1621 cgtctatgac gacatcggca tctactacga caccacccgt ccttcgcgtt tggaacaact
     1681 gattcttgcc gccgatacca tgccgtctga aaccttggct caggcgcggc aggcgatgga
     1741 tttcatcctg caacaccacc tgtccaaata caaccacgcg cccgaacttt cagacgatca
     1801 tcctttacgc tccccatcca agcctgaaac tgtcctcatt atcgaccaaa ccttcggcga
     1861 tatggccatc caatatggcg gcgcagacgc ctctacgttt gaactgatgt ttcagacggc
     1921 cttgaatgaa aacccgcaag ccgatatctg ggtaaaaacc catcccgatg ttttgtgcgg
     1981 caaaaaacaa ggctatctga cccaactggc gcagcaacac cgcgtccatc ttctggcaga
     2041 agacatcaac ccgatttctt tgttgcaaaa cgtcgataaa gtttattgcg ttacctcgca
     2101 aatgggtttt gaggcacttt tgtgcggcaa gccgctgacc accttcggcc tgccgtggta
     2161 tgccggatgg ggtgtaagcg acgaccgcca tcctgaaatc aaccgccttg ttcaaaccca
     2221 acgccgcgcc acccgcaact tgctgcagct cttcgccgca gcctatctgc aatacagccg
     2281 ctacctcaac cccaataccg gcgaagcagg cagcctcttt gatgtcatcg actatctggc
     2341 gacggtcaaa cgtaaaaacg acaaattgcg tggcgagtta tattgcgtcg gtatgtcttt
     2401 gtggaaacgc gcggttgcca aaccgttctt taacgtaccc tcttgccgtc tgaaatttat
     2461 ctcttccacc caaaaactgg caagggtcaa actgtccgac gatgcacgca tcctggcttg
     2521 gggcaacggc aaagaggcca tcgtccgctt tgccgaacaa caccacatcc ccctgctgcg
     2581 catggaagac ggctttatcc gctcggtcgg actcggctcc aacttagtgc cgccgctgtc
     2641 gctcgttacc gacgatatga gcatttattt caatgccgaa accccgtccc gtcttgaata
     2701 catcctacaa aaccaaaact tcgacgatca agactttcag acggccttga agctgcaaaa
     2761 aatgctgacc gaaaaccaca tcagtaaata caacgtcggc agctcagact tcaccgcccc
     2821 gtcaaccgac aaaaccgtga tcctcgttcc cggccaggtt gaagatgatg cgtctatccg
     2881 ctacggttcg ccccaaatct accgcaatct ggatttgctc cgtaccgtac gcgaacgaaa
     2941 ccccaatgcc tatatcatct acaaaccgca tcccgatgta gtcagcggta accgcatcgg
     3001 ccatatttcc cctgaagatg ctgcacgata tgccgaccaa accgccgaac aagccgacat
     3061 cctgacctgt ctccaatacg cagacgaaat acataccatg acttcgctga ccggttttga
     3121 agccttgttg cgcggcaaaa aagtcagctg ctacggcctg cctttttacg caggctgggg
     3181 gcttacccaa gatctgctcc ccatcccgcg ccgtagccgc agacttgagc tttggcagct
     3241 gattgccggc acgctcatcc actatcccga ctacatccac cccgaaaccc atcaggccat
     3301 aaatgcagaa accgcagccc aaatcctgat acgacaaaaa aatatgcaaa aaaacaacaa
     3361 cggattacat cgcgggtgct ttgccaaaaa attaggtaaa atcaaacaac tatatcgatc
     3421 tttcaaataa ataccatcaa agttaacgat gcgtcataaa cttgcctcta ttgcggcatc
     3481 attgcctttg catcgttaat tctcttggcg tatgcttgaa agttcaacct aaaactatta
     3541 cataaaaaac aaaaccacat tgcaacatga aacagaccgt cctcaaaaat aacctgcaaa
     3601 acctgcttga aagcgcagaa aatatcctgc tgcttcaagg ccctgtcggc gatttttttc
     3661 tgcgccttgc cgactggctg actgcaaacg gcaaaaccgt acataaattc aactttaatg
     3721 caggcgacga ctatttttat ccgcccactc aagcgcatac cgttgttttt aacgacaact
     3781 acgatgcctt tcctgagttt ttgcaagaat acatcactca acatcacatc caggccgttg
     3841 tctgctttgg cgacacacgc ccttatcacg tcattgcaaa acgcattgca aacgaaaacc
     3901 aagccagttt ctgggcgttt gaagaaggct atttccgccc ctactacatc accttagaaa
     3961 aagacggcgt caacgcattt tccccgttgc cgcgccgtgc cgactttttt cttgaacaat
     4021 tccctaagct tgcccagcaa gaatataaag cgccaacgcc ggtacacggc ggttttacgc
     4081 ccatggcaaa aaacgctatc cgttactata tcgagttgtt ccgcaatcta cgcaaatacc
     4141 ccgactacat ccaccaccgc gcacccaatg ccggccatta cctcaaaccg tggtcgctct
     4201 ccatcctcaa gcgtttgaac tactatattg aagacatcca aatcgccaaa cgtgtggaag
     4261 caggcaaata cggcaagttt tttattgttc ccttgcaggt attcaacgac agccaagtcc
     4321 gtatccattg cgactttccc agcgtccgca gcttcctgct ccatgttttg agttcatttg
     4381 ccgagcacgc gcctgccgat accaacatca tcatcaagca tcatccgatg gaccgcggtt
     4441 ttatcgacta ctggcgcgac attaaacgct ttatcaaaga acaccccgaa ctcaaaggcc
     4501 gcgtgattta tgtccatgat gtccccctgc ccgttttcct gcgccacggt ctcggcatgg
     4561 tcaccatcaa cagcaccagc ggcctgtccg gactgattca caatatgcca gttaaggttc
     4621 tcggccgtgc ctattatgat attcccggca ttactgacca aaataccttg gcagaatttt
     4681 ggaatcatcc gacaccgcct gacaaagagc tgttccatgc ctaccgaatg taccacctca
     4741 acgtgaccca aattaacggc aacttctaca gtcaggtctt ttttcctaac aaaaacacat
     4801 ccgactcttc cacaccaaca acctaacttg gcgaaagaaa agctcaggcc gtctgaaata
     4861 tttttcagac ggcctgaagc aatcaatacc ttagctactg ccatgtaaat aaaacacaaa
     4921 aacctgcatt tatcattaac aataaattac aaaaacagta taatgaccga gctgccatga
     4981 gcgcataccg actcaacctg agccctttgt aacacacaaa atatggatat acccccaggc
     5041 aaaacaatat aacaagccaa acatcctaaa gataagccgg caaggcaata cactctataa
     5101 aactgtgccg agcaaaattt ttacaaagcc ctcaaccggt atcgccgccc atatgccgca
     5161 gcatccgtct tccactttat atccgcccgc aaaccatgac cgccgctcct gatatcctct
     5221 accggcaagc cgccgccctt ttggaacaat ccaataccgc ccaagccctg cccctgttgc
     5281 aacaggcggc agagcaaggt tatgcggaag ctgctttcgt attgggcaac catctgctgc
     5341 aaaacggcca accggagcag gcactttcat ggttggaagc cgccgcggcc caacgccatc
     5401 ccaaagcact cttctccctg ctgcaacaac gcgaacacaa cggcaccccg accggacagc
     5461 ttctcaacga ctatgcctgg ctgggtgagc aggggcactc agaagcccaa ttaatcctca
     5521 tgcgttacca cgcgcaacgc aacgatccac aatcgctcta ctgggcggaa cttgctgccg
     5581 cccgatatgc cgcacctgcg tattaccatc tggcacgcca tcatcaacgc caaggcgacg
     5641 ttgaaacagc catcgaacaa tacgaaaaag cggcagcact cggcgtaact gccgcctgct
     5701 ggcaacttgg tcaaatctac ttctacggta caggtgtcag ccccaaccac gcacaagccg
     5761 aacactatct cgaaccagcc gcacaagccg gccacatcgc cgcacaaacg ctgctggctg
     5821 accttcttgc cgcccaacgc aaacctgaag ccttggaatg gtatcgtcgt gccgccgata
     5881 aggaacaagc ggaagcacag gccaagctgg cccaatacgc cctgaccggc gaactttccg
     5941 aacgcgatcc gttccaagcg gcacgatatg ccaaagccgc tgccgagaaa aaccatcctg
     6001 aagccctgaa aatcatgggc gacctctacc gctacggtct cggtatcaaa gccgacaacc
     6061 atatcgcgca agattactac caccgtgccg ccgcgctggg ttctgccgcc gcagcacaaa
     6121 aactcatcag cgacgccgcg ctgtaccatc cgcaacaata cgaacaaatc aaaactgccg
     6181 ccctgcaaca acaacaaacc gaaaccatct accgtttggc ggaagcacaa gcctgcgcca
     6241 tcggccgtcc cgccgactac aatgccgcgc gaaaaaatta catggaagct gccgggttcc
     6301 accataaaaa cgcagcggca gccttaggcc gcatctacca ttacggcctc ggtacggcgc
     6361 aagatcctcg ggcggctgca cactggtacg ccattgctgc cgaacaaaac cacccttccg
     6421 cccaatacca cctcgcctgt ttttactatc acgggcaagg tgtcggctgt catgttccga
     6481 ccgcctgcta ctggctgcag gccgccatcg gcaacggcca cacttcggcc gaatcattaa
     6541 tatccctatt agaacaatgg cgacgcgaag cacaccatgc catcggacaa aaggccgtct
     6601 gaaaagattt acactcgcat tttttgacaa tctttaacta ttcccctaat atttgccagt
     6661 tatttttcac ggacacgcca ttgttttcat ttctttctga aaacaccttg tccgcgcatc
     6721 aataccatga cactcggcgg ataacgccaa gcgttgaaac acactacatc cggaacaaaa
     6781 acggatgctc ggaaaaatat ttctaggagg tgaaacaaca tggaatggga attcaacagt
     6841 tattacacac tgattgccgc cacgctcgtg ttgctggttg gtaaattttt ggttcaaaaa
     6901 atcaaattct tacgagactt caatattccc gagccggtag ccggcggttt gattgccgct
     6961 atcgtcctgt tcgccctgca cgaggcgtac ggcgtgagct tcaaatttga gaaaccgctg
     7021 caaaatgcgt ttatgctgat ttttttcacg tccatcggct tgagcgcgga tttttcccgt
     7081 ttgaaggcgg gcggtttgcc gctggtggtt tttaccgcga ttgtgggcgg atttatcttg
     7141 gtgcaaaact ttgtcggggt cggactggct acggctttgg gtttggatcc gctcatcggt
     7201 ctgattaccg gttcggtgtc gctgacgggc ggacacggta cgtcaggtgc gtggggacct
     7261 aattttgaaa cgcaatacgg cttggtcggc gcaaccggtt tgggtattgc atcggctact
     7321 ttcgggctgg tgttcggcgg cctgatcggt gggccggttg cgcgccgcct gatcaacaaa
     7381 atgggccgca aaccggttga aaacaaaaaa caggatcagg acgacaacgc ggacgacgtg
     7441 ttcgagcagg caaaacgcac ccgcctgatt acggcggaat ctgccgttga aacgcttgcc
     7501 atgtttgccg cgtgtttggc gtttgccgag attatggacg gcttcgacaa agaatacctg
     7561 ttcgacctgc ccaaattcgt gtggtgtctg tttggcggcg tggtcatccg caacatcctc
     7621 actgccgcat tcaaggtcaa tatgttcgac cgcgccatcg atgtgttcgg caatgcttcg
     7681 ctttcgcttt tcttggcaat ggcgttgctg aatttgaaac tgtgggagct gaccggtttg
     7741 gcggggcctg taaccgtgat tcttgccgta caaaccgtgg tgatggtttt gtacgcgact
     7801 tttgttacct atgtctttat ggggcgcgat tatgatgcgg cagtattggc tgccggccat
     7861 tgcggtttcg gcttgggtgc aacgccgacg gcggtggcaa atatgcagtc cgtcacgcat
     7921 actttcggcg cgtcgcataa ggcgtttttg attgtgccta tggtcggcgc gttcttcgtc
     7981 gatttgatta atgccgcgat tctcaccggt tttgtgaatt tctttaaagg ctgattttcc
     8041 gcctttccgc caaagcacct gcaaggttta ccgcctgcag gtgcttttgc tatgatagcc
     8101 gctatcggtc tgcaccgttt ggaaggaaca tcatgtatcg gaaactcatt gcgctgccgt
     8161 ttgccctgct gcttgccgct tgcggcaggg aagaaccgcc caaggcattg gaatgcgcca
     8221 accccgccgt gttgcaaggc atacgcggca atattcagga aacgctcacg caggaagcgc
     8281 gttctttcgc gcgcgaagac ggcaggcagt ttgtcgatgc cgacaaaatt atcgccgccg
     8341 cctacggttt ggcgttttct ttggaacacg cttcggaaac gcaggaaggc gggcgcacgt
     8401 tctgtatcgc cgatttgaac attaccgtgc cgtctgaaac gcttgccgat gccaaggcaa
     8461 acagccccct gctgtacggg gaaaccgctt tgtcggatat tgtgcggcag aagacgggcg
     8521 gcaatgtcga gtttaaagac ggcgtattga cggcagccgt ccgcttcctg cccgtcaaag
     8581 acggtcagac ggcatttgtc gacaacacgg tcggtatggc ggcgcaaacg ctgtctgccg
     8641 cgctgctgcc ttacggcgtg aagagcatcg tgatgataga cggcaaggcg gtgaaaaaag
     8701 aagacgcggt caggattttg agcggaaaag cccgtgaaga agaaccgtcc aaacccacgc
     8761 ccgaagacat tttggaacac aatgccgccg gcggcgatgc gggcgtaccc caagccgcag
     8821 aaggcgcgcc cgaaccggaa atcctgcatc ctgacgacgg cgagcgtgcc gataccgtta
     8881 ccgtatcacg gggcgaagtg gaagaggcgc gcgtacaaaa ccagcgtgcg gaatccgaaa
     8941 ttaccaaact ttggggagga ctcgataccg acgtgcaaaa agagttggtc ggcgaacaac
     9001 gcaagtgggc gcaggaaaaa atcagcaact gccgacaagc cgccgcgcag gcagaccggc
     9061 aggaatacgc cgaatacctc aagctgcaat gcgacacgcg gatgacgcgc gaacggatac
     9121 agtatcttcg cggctattcc atcgattagg ggcaaaccga tgactaccgt ccccaaaagc
     9181 aggattcccg tcaaaccgct gcccgaaaaa accacagacg aagccaaagt cgaaaaatgg
     9241 cggcagctcg gtgcggaaca cggtttgtcg ggcgaatggg cagttgccgc cagattgggc
     9301 gagaacggtt ttaccgaaga acagatggaa aatatcgcca acctgttcgg cagataaagg
     9361 gaaaattgac ggaaatgccg tctgaaaccc tgttatcggt ttcagacggc attttgacta
     9421 atacggtacg caggcgcaaa acagccggct tttcctgtgt tgcctatgct gatgtttcaa
     9481 cacacaggac gatacaaaaa acgtcgccct atgtgccgtc ctgattcgga aagggttacg
     9541 ctcctcccaa
//
LOCUS       DAXHNE010000068         9443 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.068, whole genome shotgun sequence.
ACCESSION   DAXHNE010000068 DAXHNE010000000
VERSION     DAXHNE010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9443
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.068"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(175..2103)
                     /gene="dnaK"
                     /locus_tag="ACFM4Z_001234"
     CDS             complement(175..2103)
                     /gene="dnaK"
                     /locus_tag="ACFM4Z_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFC6021268.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKTEALGAASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            complement(2311..2583)
                     /locus_tag="ACFM4Z_001235"
     CDS             complement(2311..2583)
                     /locus_tag="ACFM4Z_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021269.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVALSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2769..3455
                     /locus_tag="ACFM4Z_001236"
     CDS             2769..3455
                     /locus_tag="ACFM4Z_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6021270.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            3587..3925
                     /gene="erpA"
                     /locus_tag="ACFM4Z_001237"
     CDS             3587..3925
                     /gene="erpA"
                     /locus_tag="ACFM4Z_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFC6021271.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            3942..4088
                     /locus_tag="ACFM4Z_001238"
     CDS             3942..4088
                     /locus_tag="ACFM4Z_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021272.1"
                     /translation="MPSERSDGIFTFRKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            4085..4549
                     /locus_tag="ACFM4Z_001239"
     CDS             4085..4549
                     /locus_tag="ACFM4Z_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFC6021273.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLKENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     ADGEMPEYLELHGGREDDISAPSQPEPPTRNIKPLVFGLLIAFLGYLLGKIF"
     gene            4584..6095
                     /gene="ubiB"
                     /locus_tag="ACFM4Z_001240"
     CDS             4584..6095
                     /gene="ubiB"
                     /locus_tag="ACFM4Z_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFC6021274.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGRRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKINALVDEARRQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
     gene            complement(6299..7117)
                     /gene="cysE"
                     /locus_tag="ACFM4Z_001241"
     CDS             complement(6299..7117)
                     /gene="cysE"
                     /locus_tag="ACFM4Z_001241"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFC6021275.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
     gene            7301..7879
                     /gene="grpE"
                     /locus_tag="ACFM4Z_001242"
     CDS             7301..7879
                     /gene="grpE"
                     /locus_tag="ACFM4Z_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFC6021276.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            complement(8003..8218)
                     /gene="nqrM"
                     /locus_tag="ACFM4Z_001243"
     CDS             complement(8003..8218)
                     /gene="nqrM"
                     /locus_tag="ACFM4Z_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HFC6021277.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(8244..9299)
                     /locus_tag="ACFM4Z_001244"
     CDS             complement(8244..9299)
                     /locus_tag="ACFM4Z_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC6021278.1"
                     /translation="MPSETRLPNFIRVLIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPNSEISRFNQTTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDSASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIIQGGNTQIIVPLNNRSLATSGDYRIFHVDKSGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
BASE COUNT         2121 a         2100 c         2627 g         2595 t
ORIGIN      
        1 gtttaaattt aatccactat ataaaaaatt tccagagaac cgatacaaca gttggaactt
       61 gggtttggga atattacggt agatgaactt ggaacctctg ttatgctatg gtcttttatc
      121 tcaattgaaa aaagcgcgaa tcaaacggtt cgcgcttttt tcagacggta ttaattattt
      181 tttgtcgtct tttacttctt caaagtcggc atctacgaca tcatcgtctt tctttgcaga
      241 agcattggct tgttcgcttt cgcctgcttg ggcttcagct tgtgcttgag cgtaaaccat
      301 ttcccccagt ttttggctgg ctgcgcccag cgcctcggtt ttggcatcga tagcggcttt
      361 gtcgtcgcct ttaactgctt cttcggcttc tttcagcgcg gcttcgattt tttctttctc
      421 ggctgcgtcg agtttgtcgc cgtagtcggc caaagatttt ttcacagagt gaatcagggc
      481 ttcagcttgg ttgcgggaag cgaccaattc agtcagtttt ttatcttcct cggcattggc
      541 ttcggcatct ttcaccatgc gttcgatttc ttcttcgctc aaacctgaag aaccttggat
      601 ggtgatgttg gctgctttgc cagtgccttt gtctttggcg gaaacgtgca ggataccgtt
      661 ggcgtcaata tcgaaggtga cttcgatttg cggcataccg cgcggtgcgg gtgcgatgtc
      721 gcccaagttg aactgaccca aagatttgtt ggcagaagcg cgttcgcgtt cgccttgcag
      781 tacgtggatg gttactgcgc tttggttgtc ttcggcggta gagaacactt gcgacgcttt
      841 agtcgggata gttgtgttct tttgaatcag cttggtcatc acgccgccca tggtttcgat
      901 acccaaggac agaggggtta cgtccagcag caatacgtcg ctgcggccgc cgcttaacac
      961 ttcgccttga atcgctgcgc ctacggcgac agcttcgtca gggttcacgt ctttgcgcgg
     1021 ctctttgccg aagaagtctt taacggcttc ttgtactttc ggcatacggc tttgaccacc
     1081 gaccaaaatc acgtcgtcga tgtcgccagt gctcagaccg gcatctttca atgcggtgcg
     1141 gcaaggctcg atagagcggg caatcaggtc ttcaaccagg ctttcgaatt tggcgcgggt
     1201 aattttcatc gccaagtgtt tcgggccggt tgcgtccatg gtgatgtacg gcaggttgat
     1261 ttcggtttgc tggccgctgg acaattcgat tttggctttt tcggcagctt ctttcagacg
     1321 ttgcagagcc atgacgtctt gtttcaaatc aatgccttgt tcttttttaa actcgtcaat
     1381 gatgtagtcg atcaagcgtt ggtcgaagtc ttcaccgccc aagaaagtat cgccgttggt
     1441 agccaatact tcaaattgtt tgtcgccgtc gaggttggcg atttcgatga tggaaatatc
     1501 gaaagtaccg ccgcccaagt catatacggc tactttgcgg tctttgttgt cgcctttgtc
     1561 cataccgaat gccaaagcgg ctgcggtcgg ctcgttgatg atgcgtttta cgtccaaacc
     1621 ggcgatacgg cctgcgtctt tggtggcttg acgttggctg tcgttgaagt aggccgggac
     1681 ggtaatcacg gcttcggtta ctttttcgcc caagtaagct tcggcggctt ctttcatttt
     1741 acgcaggact tctgcggaaa tttgaggagg agacagctct ttgccttgtg cttttaccca
     1801 tgcgtcgccg ttgttggctt tgatgatttc gaaaggcata gattcgatgt cgcgttggac
     1861 ttctttgtct tcaaatttgt ggccgatcaa acgtttggcg gcgtaaatag tgtttttggc
     1921 gttggtcacc gcttggcgtt tggcaggcgc gccgacgagg atttcgccgc cgtccaaata
     1981 agcgataacg gacggcgtgg tgcgtgcgcc ttctgcgttt tcgataactt tggtttgacc
     2041 gttttcggaa atggccaaac aagagttggt tgtacctaag tcgataccga ttacttttgc
     2101 catgtggata atcctatttg attttgctta ttttgagaaa tatgttggaa cattttgtcc
     2161 cgatgggctg taaatagggc gggcggcggg ctgtttcaag ctacagcatg gctataagta
     2221 tataacttta tgaatatatt ggttttatat ttgatttaat acatttggct ccaatgcatt
     2281 caagcataat gtttcaaatg gcaggcaggt ttattcatag acgatgccgg cgagcatttc
     2341 ctgttcgttc aagttgccgt actctttttc ccagtcgtgt gaagactcga tgatgtcgca
     2401 ttctttggaa agggcgactt gttctgcatc catatctttg gcgttcagta tgttgaattg
     2461 ttcgcacagg gatgcggata aagtgatgtc aggctgtttg gcttcagaac ggttttcttg
     2521 gaaggcaaag cagaatgcgg taaatgccgc agcatagata agatatttgc cggttttctt
     2581 cattttctat cctttttctg tcaattcagg attaaaccta tggaaaatct taaaaattat
     2641 gtattgagta agaaaaatca taatttaaat ttagtttatc atagttgttc cgtttttggg
     2701 atagctaagg taaaatatat ttcatgttta ctttagatga ttgaatgaag gggagcggaa
     2761 ggatatttat ggaaaccttt aaagacagac tggttttttt atggaaaagc gaagcgaagc
     2821 aggcaaaaat cgcatccgat attgaaatga cgattgcggg cttcagcagg atatggaatg
     2881 aaggcggttt gccgaagtct gaaacattaa aaaaaatcaa gcagttgaag gggtgtagta
     2941 tcgattggct gctgaccggg gagggtaatc cgtttccgga tgaagcccca aaaaaatccc
     3001 ttgcttacga tactttgggc aatgaagtcg atacggacga gtttgtcttc gtgccgagat
     3061 atgatattcg ggcggctgcg ggatacgggc agtttgtcga tcatgaggaa ccggtattta
     3121 caatggcgtt cagacggcat tggattgaga attatgttac ccgcgatacg aaaaacctgt
     3181 ctgtaatttc cgtcaagggg gattcgatgg agggggtttt gaatgacggc gattcgattt
     3241 tggtcaatca tggtgaaaat acgccgaggg acggtctgta tgtgttgcgg attaatgaaa
     3301 atctgctggt taaacgttta cagattgtac cgggcgggat tatcaatgtg atttctgcaa
     3361 acgaggctta tcctgctttt gaaatcaatt tgaacgattt gaccgatgat gtggagatta
     3421 tcgggcgtgt cgagtggttc ggcaggacga tttgagtttg gggcttgaaa ttgcaggcgg
     3481 tcaaacttat ctattggaac aattcctttt tcaaaggcga agcctgcttg cctttgaagg
     3541 gggtttgaga gagaatgcag aaaatattat attaaggaat aacaccatgt cggatgaaag
     3601 ccctattatt tttactgaca gctgctgtgc caaagttgcc gatttgattg ccgaagaaaa
     3661 caatcccgat ttgaaattgc gggtttttgt caatggcggc ggctgttcgg gtttccagta
     3721 cggatttact tttgacgaaa tcaaaaacga cgacgatttt gaaattgaga aaaacggttt
     3781 ggtctttttg gtcgatccga tgagctatca atatctggtc ggtgcggaaa tcgactatac
     3841 ggaaagtttg cagggttcgc aattcgtcat ccgcaatccg aatgcggaaa caacctgcgg
     3901 ttgcggatcg tcgttttccg tatgaccgct tggtttgtgt gatgccgtct gaacgttcag
     3961 acggcatttt tacttttaga aaatatatta tcgggatgaa ttcacatata atccgattgt
     4021 ttgaagatga atcgggtttc ccgaaaggaa cgggcggaac ggtatcaggc gtatttgttc
     4081 ccttatgatt gagatgagta aagattaccg aaacgatttg tacgatgtat atgtttctta
     4141 cccgccccaa gtggatcgcg ggcttatccg ggagtgcctt aaggagaatc tcggtgagga
     4201 aaaggcggaa ggattgatcg aatcgctcga ttccaaacct caagtgctgg ttgaggaaaa
     4261 atgcacttgg gcgaaacggg aagagttgca tgattatttc agctatttgg gtttggatat
     4321 tattacccgg agatatatgg agttggaaac ggtcgtgccg ccggaggaag gtgagggcga
     4381 aggagagggc gcggatgggg aaatgcccga atatcttgaa cttcatggcg ggcgggaaga
     4441 tgatatttcc gcaccttcgc aacccgaacc gccgacccgc aatatcaaac cgctggtttt
     4501 cgggctgctg attgcctttt tgggctatct gctcggtaag attttttgat tgtccgataa
     4561 atgctgtatt cgggatttta tatatgaaat ggttgaaacg cctgacggtc attgtcggga
     4621 ctttttaccg ctatcggctg gcaggtctgt gtgcttcgct gatgggtagc ggttggatat
     4681 gcgctctgct gaaaatgatg ccgcagtcgt ccaaattgaa aaacgaaccg cctgctgtcc
     4741 gtctgcgcct tgccttggaa agcctggggc cgattttcat caagttcggg caggtattat
     4801 ctacgcgccc cgatttgatt ccgcacgatt acgccgtcga actggcaaag ctgcaagaca
     4861 aagtgccgcc ttttgacgcg cggctttcgc gtgaacaaat cgaaaaatcg ttgggtcagt
     4921 ccatcgaaaa gctgtatgcg gaatttgaaa ccgagcccat cgccagcgcg tccatcgccc
     4981 aagtacacaa agcccgcctg cattcgggcg aacgagtggc ggttaaagtt ttgcgcccca
     5041 accttttgcc ccttatcgaa caggatttgt cgctgatgcg tttcggcgcg gcctgggtcg
     5101 aacgcttgtt ttcagacggc aggcgtctga agccgctcga agtggtggcg gagttcgaca
     5161 aatacctgca cgacgagttg gatttgatgc gcgaagccgc caatgccgga cagctcgggc
     5221 gcaatttcca aaacagcaat atgctgattg tgcccaaggt gttttacgac tactgcacca
     5281 gcgacgtgct gacaatcgaa tggatggacg gcacgccggt tgccgacatc gccaaactca
     5341 aagcagacgg catcgatttg cacaaactcg ccgattacgg cgtggaaatc tttttcacgc
     5401 aggtcttccg cgacggcttt ttccacgcgg atatgcaccc cggcaatatt ttggttgccg
     5461 ccgacaaccg ctacatcgcc ctcgatttcg gcatcgtcgg cacgctgacc gattacgaca
     5521 aacgttatct cgccatcaat tttctcgcct ttttcaaccg cgattaccgg cgcgtcgcca
     5581 ccgcccacat cgaatcgggc tgggtgcctg ccgacacgcg tgcggaagaa ttggaagccg
     5641 ccgtccgcgc cgtgtgcgaa ccggtgttca acaaaccgat ttcgcagatt tccttcggct
     5701 tggtgctgat gcgcctgttt gaagtcagcc gccgcttcaa tgtcgaaatc caaccgcagc
     5761 ttgtgctgct gcaaaaaacg ctgctcaaca tcgaaggctt gggccgccag ctcgatcccg
     5821 atttggactt gtggaaaacc gccaaaccgt ttttggtgaa atggatgaac gggcaggttg
     5881 gccctaaagc cctttggcgc aacctcaaaa acgaagcccc cgactgggcg caaatcattc
     5941 cttcattgcc gcgcaaaatc aatgccttgg tcgatgaggc gcgccggcag gaaatgcgcg
     6001 atgcctatat tcatttggtt aaagtgcagc agcggcaaag cctgtggctg gctgtgattg
     6061 cggttgtttt gctgctgatt ttgcttttga aataggcttt gtccgaatta tcgcccgact
     6121 ccgcccgttt acaaggaaac cggttatttc cggttgcgtg ggaatcgggt gtatcgaata
     6181 aaaggcattt tgtccgactg gcaagtgccg acatcggcgg catatcaagg cgcaggcttg
     6241 aagcgggcaa tgtcgtctga agcccgtttg gcgtttcaga cggcattggt gcggatattc
     6301 aaatcataaa gtcgatttcg gtaaactgga tattttgatc catatccgcc gacggtgttt
     6361 tgagcgatcg cgccacgggt ttggcgggta cgccgacaac cgtgatggac ggcggcacgt
     6421 ctgaaaccac gacgctgccc gccccgattt tggcattgct gccgatgcgg atattgccca
     6481 atatcgaggc gtttgcgccg atcatcacgc cgtcgccgat tttagggtgg cggtcgccgc
     6541 cttctttgcc cgaaccgccg agcgttacgc cgtgcaaaat cgaaatattg ttgcccaaca
     6601 cggcggtttc gccggcaaca aagccggtgg cgtggtcgag catcagcccg tatccgaaac
     6661 gggcggcggg atggatgtcc acgccgaata cttcggacat acggttttgc aggaaatacg
     6721 ccagcgtttt gcgcccgtcg agatacagcc ggtggttgat gcggtgtgcc tgaatcgcgt
     6781 ggaagccttt gaaatataaa agcggcagcg aatattcgtc gcaggcggga tcgcgttcgt
     6841 agatggcttt taagtctgct tcgacgcatt tgccgatttg ggtgtcgctg cccaacgcct
     6901 gctggtagat ttcaaacagc gcgcgcacgt ccataatcgg gctgccgagt ttgctggaaa
     6961 ggtggtaggc aaggacggag ccgagggact cgtggcgcaa cacggtttgg tgcaaaaaac
     7021 ttgccagcat cggttcggcg gagaccgcgg ccgcggtttc ttcgcggatg gtgtgccaga
     7081 ggtcgaaacc ggttgtgttt aaatggtctt ttttcatgag tgatgacgtt tgaaaatcga
     7141 tatggtcggc agtatcttac cgtctatatt attttttcgg taggggattt gaaaatgaat
     7201 ttgaaattct ctgcttttgc ttgaagtttc ttgaaaatgt ccttatcttg cgcgggtaat
     7261 aactggattt tgatttccaa tttgttttaa gggatacgat atgagcgaac agacacagca
     7321 gcaaaacagt gaagaagcgg ttgaaaatgt ggaggcggtg gaaaccgtcg agacagtagg
     7381 aaatgcggac ggtgtgcagg aacaggctgc cgcagagccg gcttatgagg atttgcaggc
     7441 gcggattgcc gagctggaag cgcagttgaa agacgagcag ctgcgcgctt tggcaaacga
     7501 gcaaaacctg cgccgccgcc accagcagga aattgcggat acgcacaagt tcgccggaca
     7561 gaagtttgcc gtggaaatgc tgccggtcaa ggattacctg gaaatggcgc ttttggatca
     7621 gagcggcaat ttcgatgcgc tgaaaatggg cgtgcagatg actttgaacg agttgcagaa
     7681 agcatttgat gctacgcaaa tcaaggaaat caatcctaaa gcgggcgata agctcgatcc
     7741 gaatatccat caggcgatgc aggcggtggc aagcgaacag gaacccaata ccgtggtggg
     7801 cgtgatgaag aagggttata cgctgtccga ccgcgtgttg cgcccggcta tggttacggt
     7861 ggcacagaag gaagcctgaa ggcgtctggg gaataatctg atttatttcc tgaagcgcgt
     7921 tttgcgtata aaccgatcga agtaaagcgg caatgccgtc tgaacccgcc tgtcgggctt
     7981 cagacggcat tttgtttcgg cgttaacggt caacccgtat gccgcctgcc tgtttttctt
     8041 catccagttt cttttgcagg gtgtcgcaag gtgtgtcgca gtcgcacatt tttttcatac
     8101 ccaaggcagt aatgccgccg caactgcctt tgatgctgcg tttggagaaa atatagccga
     8161 ccgccatacc gatgatgacg gtcaggaaga tgccgaaggt aaggagcagg gttttcatgg
     8221 tgtttcctaa tcggtttgta tgtttagcgg agcagttttt caaattcgga agacatcgcg
     8281 gtgcggtagc cgcctttatc cctgacaatc aggaaaacag cgagtttttc gcgctctgcc
     8341 agctttaagg cttcggtttc gcccaatacg aataatcctg tggacaagcc gtccgccgtc
     8401 atcgcactgt ctgcgaccac gctgatggag gcgaggttat ggctgatggg tcgtttgttg
     8461 ttcggattaa tgatatggga gaggcgtttg ccgcttttat cgacgtggaa aatacggtaa
     8521 tcgccggaag tggcaagcga acggttgttc agcgggacga taatctgcgt attgccgcct
     8581 tggatgatat tgggttgctc gataccgatg cgccacggtt cgccgcgcgc gtttttgcct
     8641 ttgccgtgca actcgccgcc gatttcgacc agataatttt gaatgccgta tttttccagt
     8701 tcgcccgcaa ctttatcaac gccgaagcct ttggcaatcg aagataaatc caaataggct
     8761 ttggggtggg ttttgctcaa ggaggcggaa tctttgcctt gtttcaaaat gattttgtct
     8821 atgcccgtat aagatgccgc ctgtttgatt tgttccggcg acggttcacg ggtaacggat
     8881 ttgtcgggcc cgaatcccca aaggttgacc aaagggccga cggttacgtc cagcgcgccg
     8941 tgtgtcaggc ggttcaggcg gacagcttcg gcagtaacgt gtgcgaagtc gcttgaaatg
     9001 cggaggggtt tgccggccgt ggtttggttg aaccggctga tttcggaatt aggctgatag
     9061 gtggacatct gccggttgac ttctttaagc gcgtcatcga tgcgcttttg tatttcggca
     9121 ggtgagggga gtttgtcccg attatttgaa aggtatttga cggtataggt cgtgcccatc
     9181 gtttcgcctt gcagggtaac ggtttgcgcg gtttgttccg aacaggcgtt caggaagatg
     9241 aaacccaggg caaatatcaa gacgcggata aagttcggca ggcgtgtttc agacggcata
     9301 gtgtttgacg gttttggcaa atggtttgaa ttatatcgca aaacggccga tatgtttcta
     9361 tgccgatgcc gtctgaaggg catcaggggc atcgcccgct gtttgggggc ggtaataaaa
     9421 aaacaaaccg gtgttttaca aac
//
LOCUS       DAXHNE010000069         9340 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.069, whole genome shotgun sequence.
ACCESSION   DAXHNE010000069 DAXHNE010000000
VERSION     DAXHNE010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9340
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.069"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(135..470)
                     /locus_tag="ACFM4Z_001245"
     CDS             complement(135..470)
                     /locus_tag="ACFM4Z_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC6021279.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            599..1504
                     /locus_tag="ACFM4Z_001246"
     CDS             599..1504
                     /locus_tag="ACFM4Z_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFC6021280.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGNGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     MVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1566..2054)
                     /locus_tag="ACFM4Z_001247"
     CDS             complement(1566..2054)
                     /locus_tag="ACFM4Z_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC6021281.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2201..3103
                     /locus_tag="ACFM4Z_001248"
     CDS             2201..3103
                     /locus_tag="ACFM4Z_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6021282.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPCWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3144..4274)
                     /locus_tag="ACFM4Z_001249"
     CDS             complement(3144..4274)
                     /locus_tag="ACFM4Z_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6021283.1"
                     /translation="MAKHLPLAVLTALLLAACGGSDKPPAEKPAPAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4478..7447
                     /gene="alaS"
                     /locus_tag="ACFM4Z_001250"
                     /pseudo
     CDS             4478..7447
                     /gene="alaS"
                     /locus_tag="ACFM4Z_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343549.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
     gene            7708..8139
                     /locus_tag="ACFM4Z_001251"
     CDS             7708..8139
                     /locus_tag="ACFM4Z_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021284.1"
                     /translation="MSISLIGLHITIAIILFFTTNFMGKKSSIFGYYQLSFSEENHSP
                     AFNIFYRAFTPILFIVIFSWVVTSLEIPISLEKINYVVIYYFIIRLLSVFVFEKTHIV
                     NWFNQLTIPILSITLSFIVYNKMILPKSFLLPSSQEVATTF"
     gene            8439..8726
                     /locus_tag="ACFM4Z_001252"
     CDS             8439..8726
                     /locus_tag="ACFM4Z_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021285.1"
                     /translation="MQVTSAEYLSDEESIKKGGNILMEKYNEKYNESIDGNKTLYKSY
                     YESRRESIKNYNPDAKYINEIESIYMMLGEIYPNAPDFMSPHFEGDCSEGE"
     gene            9214..>9340
                     /locus_tag="ACFM4Z_001253"
     CDS             9214..>9340
                     /locus_tag="ACFM4Z_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6021286.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQK"
BASE COUNT         2441 a         2344 c         2201 g         2354 t
ORIGIN      
        1 ttcacgctta ccccttgttt ctcaaatgcc gtctgaaaaa aacggctgta tatgttgtct
       61 gcaaaatatc gggaagcata acagacaaaa tgccgtctga aatattttca gacggcattt
      121 caatccgatt cggattattt ttgcgtttca accgcttcca atgcacgcag ggcgtaagtg
      181 taagcggcac cggcattcag ggcgatggcg gttgccaatg cgccggcgat ttcgctgtcg
      241 gtcgcaccgg ctttggcggc ggcggcggcg tgaacactga tgcagctctc acaacgtgtg
      301 gtaatggcaa cggcgatggc aatcagttcg cgcgttttgg catcaagtgc ttcggcggcg
      361 gcggcctgtt ccaatgcgcc gtaggcctgc agcattttag gatgcgcctt acccagctcg
      421 ccgaacgatt ttttaaccaa tgcggtatgt tctttccaat ctttaaacat tttcttttcc
      481 tttctcttgc gtttaaccct gatacgcgct tgcgtatctg ttttcgatgt gcgtattatt
      541 gcaattattc agttgtgttt ctcgtttaat catctcattt tatggttcaa aaagatttat
      601 ggacattctg gacaaactgg tcgatttcgc ccaattgacg ggcagtgtgg atgtgcagtg
      661 ccttttgggc ggacaatggt cggtacggca tgaaaccttg caacgcgaag gactggtaca
      721 cattgttaca tcgggcaacg gctatctctg catcgacggc gaaacttccc cgcgtccggt
      781 cagtacaggg gatattgtat ttttcccgcg cggcttgggt catgtgttga gccacgacgg
      841 aaaatgcgga gaaagtttac aaccggatat gcggcagcac ggtgcgttta cggtcaaaca
      901 gtgcggaaac ggacaggata tgagcctgtt ttgcgcccgt ttccgctacg acacccacgc
      961 cgatttgatg aacgggctgc ctgaaaccgt ttttctgaac attgcccatc cgagtttaca
     1021 gtatgtggtt tcaatgctgc aactggaaag caaaaaacct ttgacgggga cggtttccat
     1081 ggtcaacgca ttgtcgtccg tcctgctggt gcttatcctg cgcgcctatc tcgaacagga
     1141 taaggatgtc gaactctcgg gcgtattgaa aggttggcag gacaaacgtt tgggacattt
     1201 aatccaaaag gtgatagaca aaccggaaga cgaatggaat gtcgacaaaa tggtggcggc
     1261 tgccaatatg tcgcgcgcgc aactgatgcg ccgtttcaaa agccgggtcg gactcagccc
     1321 gcacgccttt gtgaaccata tccgcctgca aaaaggcgcg ttgctgctga aaaaaaaccc
     1381 ggattcggtt ttgtcggtcg cactgtcggt aggctttcag tcggaaacgc acttcggcaa
     1441 ggcgttcaaa cggcaatatc acgtttcgcc gggtcaatac cggaaagaag gcgggcaaaa
     1501 ataaatcggg gcttcaaacg caaatgccgt ctgaaaaggc tttcatacag catttgcgta
     1561 ccgcgtcatt tcaagggctg catcttcatc acttccatca aaaagttggt aaatgcgggg
     1621 ttgttgggtt tgacatccat atttttccaa cgctgctgcc agccgcgcaa ggcattctgg
     1681 atatacagct tggactgttc cgtattgatt gcgccccgct ggctgtctat cgccgaacgc
     1741 aggtagattt catacatact gtcatcgacg gcattgcgtc cgaccaggcg ttttctgaag
     1801 ttgttcagat attgcgccgc ctgaaccttg gtcattttac cgatacccac ctgatagccc
     1861 aagcgcgtcg cttcatcgct gattttggca acatccgtcc aatgcgaaga ggcaaggcgg
     1921 aaaccttttg caggtgcttc cgttttgacg gtattgatag gattcacggg gatttccgtc
     1981 aatgtgggca cataaataga ctggcagccg gaaagaactg ccacaatgga aagagggata
     2041 aggtattttt tcatgccccc attataatca agtttgcctt gagaaaacaa attgttcggc
     2101 aagaaaaata aaatttcggc atcagaagca ggcaaaaaca cattccacaa gccttgccgc
     2161 aaggtttaca atccgaccgt ccttatcgca acgaccgttt atggataccg caaaaaaaga
     2221 cattttagga tcgggctgga tgctggtggc ggcggcctgc tttaccatta tgaacgtatt
     2281 gattaaagag gcatcggcaa aatttgccct cggcagcggc gaattggtct tttggcgcat
     2341 gctgttttca accgttgcgc tcggggctgc cgccgtattg cgtcgggaca ccttccgcac
     2401 gccccattgg aaaaaccact taaaccgcag tatggtcggg acgggggcga tgctgctgct
     2461 gttttacgcg gtaacgcatc tgcctttggc caccggcgtt accctgagtt acacctcgtc
     2521 gatttttttg gcggtatttt ccttcctgat tttgaaagaa cggatttccg tttacacgca
     2581 ggcggtgctg ctccttggtt ttgccggcgt ggtattgctg cttaatccct cgttccgcag
     2641 cggtcaggaa acggcggcac tcgccgggct ggcgggcggc gcgatgtccg gctgggcgta
     2701 tttgaaagtg cgcgaactgt ctttggcggg cgaaccctgc tggcgcgtcg tgttttacct
     2761 ttccgtgaca ggtgtggcga tgtcgtcggt ttgggcgacg ctgaccggct ggcacaccct
     2821 gtcctttcca tcggcagttt atctgtcatg catcggcgtg tccgcgctga ttgcccaact
     2881 gtcgatgacg cgcgcctaca aagtcggcga caaattcacg gttgcctcgc tttcctatat
     2941 gaccgtcgtt ttttccgctc tgtctgccgc attttttttg ggcgaagagc ttttctggca
     3001 ggaaatactc ggtatgtgca tcatcatcct cagcggtatt ttgagcagca tccgccccac
     3061 tgccttcaaa cagcggctgc aatccctgtt ccgccaaaga taaaaaatgc cgtccgaaca
     3121 tccttcagac ggcatatcgg gctttatttc cccgccttca catcctgcca ctggcgcacc
     3181 ataaacttca atgccgccgg ctggataggc accatgataa agctgttttt caaatcctcc
     3241 tcggttggga aaatcgtatt gtcgttttta aattcgtctt ccatcagctc acgcgcaggc
     3301 ttgctcgaag gcgcgtaagt aacgaaattg ccgtttttcg ccgacacttc cgggtcgagg
     3361 aagtcgttga tgtatttgtg cgcgttggcg acgtttttcg catctttcgg aatcacgaaa
     3421 gaatccaccc aaatccccac gccctctttg ggcatcatca cgcggatttt ttccttgccg
     3481 cccgcttctt cggcacggcg tttggcgatg ttcaaatcgc cgccgaaacc gattgttacg
     3541 caggtatcgc cgcgcgccaa atcatcgata aagccggacg aagtaaagcg tttgatattg
     3601 gggcggtttt tcttgagtag ggcggttgcc tccctgatgt cttccgtatt gctgctgttc
     3661 gggtttttac ccaaatagtt caacaccata ggatagattt ccgccgcgct gtccaaatag
     3721 ctgatgccgc attgcttgag tttggacgtg tattcggggt cgaacaccaa atcccactgg
     3781 ttgtccggca gcttgtccgt acccaaagcc tttttcacgc gttcggtatt gatggcgaag
     3841 gtatttgtcc cccaataaaa cggcacggcg tattcgtggc cgggatcgac cccgtccatc
     3901 agcctcatca tttcgggatt gaggtgttta taattgggaa tcagcgattt atcgattttc
     3961 tgatacgcac ctgccttaat ctgcctgccc acaaacgcat tggacggcgc gacaatgtcg
     4021 taaccggatt ttccggtcag caccttgctt tccagcgttt catcgctgtc gtacacatca
     4081 taagtaacct tgatgccgtt tttcttttca aaatcggcaa cggtttccgg atcgacgtat
     4141 tccgaccagt tgtaaatttt caatacgttt cggttttccg ccggcgccgg tttttcggca
     4201 ggcggtttgt ccgaaccgcc gcacgctgca agcagcaaag cagtcaggac ggcgagtggc
     4261 agatgtttgg ccattatcat tccttgcata tcgggttgga gaaagcggcc attatagccg
     4321 atattgggaa cagggcttca gacggcattc aaaatcccgc aatgccgtct gaaagccgcc
     4381 gcttccatag ctagaaacag ggatttgcgg taagataccg ccgttcgttt tccctgcttt
     4441 taccatgaca agacatttga gagacattga aaaaattatg aaaacctccg aactgcgcca
     4501 aaaattccta aaattttttg aaaccaaagg ccacaccgtc gtccgctctt ccagcctcgt
     4561 gccgcacgac gacccgaccc tgctgtttac caacgcgggc atgaaccagt ttaaagacgt
     4621 attcttaggt ttcgacaaac gcccgtacag ccgcgccacc accgcgcaaa aatgcgtacg
     4681 cgcaggcggc aagcacaacg acttggaaaa cgtcggctac accgcccgcc accacacctt
     4741 ctttgaaatg atgggcaact tctccttcgg cgactacttc aaacgcgacg ccatccactt
     4801 cgcttgggaa tttctgactt cccccgaatg gctcaacatc cctaaagaca aactgttggc
     4861 gaccgtttac gcggaagacg acgaagccta caacatctgg ttgaacgaaa tcggtatgcc
     4921 gtccgagcgc atcgtccgca tcggcgacaa caaaggcgcg aaatacgtgt ccgacaactt
     4981 ctggcaaatg ggcgacaccg gcccttgcgg cccctgctcc gaaattttct acgaccacgg
     5041 cgaagaaatc tggggcggca ttcccggcag ccccgaagaa gacggcgacc gctggatcga
     5101 aatttggaac tgcgtattta tgcagttcaa ccgcgacgaa caaggcaata tgaatccgct
     5161 gcccaagccg tccgtcgata ccggtatggg cttggaacgt atggccgccg tcatgcagca
     5221 tgttcacagc aactacgaaa tcgacttgtt ccaagacctg ctcaaagccg ttgcccgcga
     5281 aaccggcgca gctttcagca tggatgaacc cagcctgaaa gtcatcgccg accacatccg
     5341 ctcctgctcg ttcttgattg cagacggcgt gttgccttcc aacgaaggcc gcggctacgt
     5401 attgcgccgc atcatccgcc gcgccgtgcg ccacggttac aaactcggtc agaaacaagc
     5461 atttttctac aagctcgtgc ctgatttggt gaaagcgatg ggcgacgcgt atcccgagtt
     5521 gaaagagaag cagacgcaaa ttatggaagc cctgcgcgcg gaagaaagcc gcttcggcga
     5581 aacgctggaa aaaggcatgg gcttgttcaa ccaagtctat aatgaattga aattcaatca
     5641 aatttttgat cttctggcga aagaatttaa ggttacacct gttgaagata ttgatcttga
     5701 tatgtttagt acaagaaatt ttgtaggaag aagtgctgaa attccttata tatcaaatca
     5761 acaccctaac gtggattatc ccgctcctat ggggaacgta aatgaaaaaa tttatgctat
     5821 gccagtgaga ttaacagatg gaagaattgg tattaatatt tttcctactg atgaaactgc
     5881 acgcctaatc cttagcaatg attggaatag tttgcgcttc tattcttttt gtaattcgtt
     5941 gattacttat tatcaacaga aaggtcaatt agaaacatta aaattgaacg gcgaacacat
     6001 cttcaaactc tacgacacct acggtttccc ctacgacctg actgccgaca tggcgcgcga
     6061 actgggcatc gaactggacg aagcaggctt cgagcgcgaa atggaagccc aacgcgcacg
     6121 cgcacgcgcc gcccaaagct tcaaagccaa cgcccaactg ccttatgacg gtcaagacac
     6181 cgagtttaaa ggttatagcg aacgccaaac cgaatccaaa gtcctcgccc tctacaaaga
     6241 cggcgagcaa gtcaacgaat tgaacgaagg cgacagcggc gcagtcgtta tcgactttac
     6301 cccgttctat gcagaatccg gcggccaagt cggcgacgtc ggctatatct tctcaggcga
     6361 aaaccgcttt gaagtacgcg atacccaaaa aatcaaagcg gccgtattcg gccaattcgg
     6421 tgtacaaacc tcaggccgtc tgaaagtcgg cgacagcgtt accgccaaag tggacgacga
     6481 aatccgcaat gccaatatgc gcaaccacag cgcgacccac ctgatgcaca aagccctgcg
     6541 cgacgtattg ggcggacacg tcgaacaaaa aggctcttta gttaccgccg aatccacccg
     6601 tttcgacatt tcccatcccc aagcggtcac tgccgaagaa atcgccgaag tcgaacgccg
     6661 cgtcaacgaa gccgttttgg cgaacgttgc cgtcaatgca gccattatga gcatggaaga
     6721 cgcgcaaaaa accggcgcga tgatgctctt cggcgaaaaa tacggcgaag aagtgcgcgt
     6781 actgcaaatg ggcggtttct ctaccgaatt gtgcggcggc acacacgttt cacgcaccgg
     6841 cgacatcggc ctcttcaaaa tcatcagcga aggcggtatt gccgcaggcg tgcgccgtat
     6901 cgaagccatc accggcctga acgcactcaa atgggcgcaa gagcaagagc gtttggtgaa
     6961 agacattatt gccgaaacca aagcccaaac cgaaaaagac gtactggcaa aaatccaagc
     7021 aggcgcggca cacgccaaag cattggaaaa agaattggca cgcgccaaag ccgaactcgc
     7081 cgtccacgca ggcgccaaac tcttggacga tgcaaaagac ttgggcgcag ccaaactcgt
     7141 tgccgcccaa atcgaagccg acgcagccgc cctgcgcgaa atcgttaccg atttaaccgg
     7201 taaatccgac aacgccgtga ttcttttagc ggcagtaaac gacggcaaag tctccctgtg
     7261 cgccggcgta tccaaagccc tgactggaaa agtgaaagca ggcgacttgg tcaaattcgc
     7321 agccgaacaa gtcggcggca aaggcggcgg ccgtcctgac ttggcgcaag ccggcggcac
     7381 ggatgccgac aaattgcccg ccgtgttgga tagcgtgaaa gactgggtcg gcgcgaagct
     7441 ggtttgatgt gggaaaggca gcctgaaagg tttcaggctg ccttttgtgc aaagaggccg
     7501 tctgaaaggt ctcgtttgcc gtaggttggg tcgcgaccca acaaattttg tgaagtataa
     7561 aaatgttggt catgacccaa cctacctgcc tttttgtaca aagaggctat ctgaaaggcc
     7621 ttgtttgccg tatggtgggt cgcgacccag cagattttta ttagggtatg acccaagcta
     7681 cttgctacga taaaaaagga tttttaaatg agcattagcc ttattggact acacattacc
     7741 atagcaatca ttttgttttt tactacaaat tttatgggaa aaaaatcatc tatatttggc
     7801 tattaccaac tgtcttttag cgaagaaaat cactctccgg catttaatat tttttacaga
     7861 gcatttaccc ctatattatt tatcgttatt ttttcttggg ttgttactag tcttgaaatt
     7921 cccatttctc ttgaaaagat aaactatgta gtaatttatt attttataat tagattgtta
     7981 tctgtatttg tttttgagaa aacacacata gttaactggt ttaatcaact aacaataccc
     8041 atactatcca taacattatc atttatagta tataacaaaa tgattttgcc caaaagtttt
     8101 ctacttccat cctcacaaga agtagctact actttttgaa tagcgcttgg tggttacata
     8161 tataatatat taaataatga atcagggcat ttaaaatctt ataaagaaag aagagtaaat
     8221 tatgtaaaac acatgcacaa aaaatttgaa agttattttg gtaaaattat agataaaata
     8281 atcaaagagg atagttataa taatgatgat tttttaaccg ataagaaaaa agcactaata
     8341 tattcagttt taatttatga gaattttaat aggggactag tttatagata ttttgaaaaa
     8401 aattattttg tactggtaga ataaaaacat ttggaataat gcaagtaacc tcagcagagt
     8461 acctttccga tgaggaaagt ataaaaaaag gcggaaatat tcttatggaa aaatacaatg
     8521 aaaaatataa tgaatctatt gatggcaata aaactctcta taaatcatat tatgaatcaa
     8581 gaagagagag tattaaaaac tacaacccag atgcaaaata cattaatgaa attgaatcaa
     8641 tttacatgat gcttggagaa atctatccaa atgcaccaga cttcatgtca ccacattttg
     8701 agggggactg ctctgagggg gaataaaatc attatttatt ctttattagt tattagcagg
     8761 atttgtcggg cataaatgcc cgacctacaa attcaatttt ttcaaacctc tgccaaatat
     8821 tttcatcttt gcaaggctgt ctgaaaaccc aaaccccatt ttcagacggc cttttttcgc
     8881 taaaatcccc ataccgttca atccgaaaac acaggagaat catcatggaa gttaccatct
     8941 ccgccatcat caatggcgaa tttgccgacc aatacggcaa gcgcggtagt cagtttaatg
     9001 aaaacgggat gctgatttaa ttctatttcc tttgaaacta ccaataacct gcctccatca
     9061 taaaactaaa agcaagccgt agcctgcatt cccacaaacc gcgtgcgttg ccatgtcaca
     9121 caccctacct gcgggcgacg caaaccttaa gagacctttg caaaattccc caaaatcccc
     9181 taaattccca ccaagacatt taggggattt ctcatgagca ccttctttca acaaaccgcc
     9241 caagccatga ttgccaaaca catcgaccgt ttcccgctat tgaagttgga ccaggtgatt
     9301 gattggcagc cgatcgagca gtacctgaac cgtcaaaaaa
//
LOCUS       DAXHNE010000070         9303 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.070, whole genome shotgun sequence.
ACCESSION   DAXHNE010000070 DAXHNE010000000
VERSION     DAXHNE010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9303
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.070"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            3..353
                     /locus_tag="ACFM4Z_001254"
     CDS             3..353
                     /locus_tag="ACFM4Z_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021287.1"
                     /translation="MAVSHHQGYKSAFAKQQAVIDKMERDKAQALLLSAQNYARELEQ
                     ARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKKEIENVLTQDRKNASGGCIDGFGS
                     HGLQLYNRALGYGN"
     gene            250..555
                     /locus_tag="ACFM4Z_001255"
     CDS             250..555
                     /locus_tag="ACFM4Z_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021288.1"
                     /translation="MSLLKTVKMQAAVALTALALTACSSTTAPSATEIKVVEKAVMPT
                     PPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQNQAWRDWAGNHSRKVGN"
     gene            complement(600..770)
                     /locus_tag="ACFM4Z_001256"
     CDS             complement(600..770)
                     /locus_tag="ACFM4Z_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFC6021289.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            783..1001
                     /locus_tag="ACFM4Z_001257"
     CDS             783..1001
                     /locus_tag="ACFM4Z_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021290.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            998..1336
                     /locus_tag="ACFM4Z_001258"
     CDS             998..1336
                     /locus_tag="ACFM4Z_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021291.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFHLEYPITNYDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYA
                     VRIVFGDVPV"
     gene            1348..1614
                     /locus_tag="ACFM4Z_001259"
     CDS             1348..1614
                     /locus_tag="ACFM4Z_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021292.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKADAKADSTNIAAALERIENKLEKVNEKLDRKADKS"
     gene            1611..1808
                     /locus_tag="ACFM4Z_001260"
     CDS             1611..1808
                     /locus_tag="ACFM4Z_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021293.1"
                     /translation="MSDPILDALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            1811..2317
                     /locus_tag="ACFM4Z_001261"
     CDS             1811..2317
                     /locus_tag="ACFM4Z_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HFC6021294.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     VEVEKKFG"
     gene            complement(2314..2904)
                     /locus_tag="ACFM4Z_001262"
     CDS             complement(2314..2904)
                     /locus_tag="ACFM4Z_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021295.1"
                     /translation="MNWLNRGIQEKILRVLSDNYPNCLKATKVYNSLFPSSSLPITSN
                     IPVDKQGLGLALTLGCEIQSQHLATLQSDEFQYFLKNIYYLEESGLIEITSIDSLHKN
                     FDCKINHKGIDFLTDDGGLSAILGVVTVKLHSDTIQALITAKIDQAEISDSEKSWLKK
                     ELGKIKDTALSTLTENAINAIPAATLITLLKKFVGL"
     gene            2917..4539
                     /gene="terL"
                     /locus_tag="ACFM4Z_001263"
     CDS             2917..4539
                     /gene="terL"
                     /locus_tag="ACFM4Z_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFC6021296.1"
                     /translation="MKTKEFLKSLAELAASLRQVIEAEVDGFDASPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTK
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTVLDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWHGIKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYQANKDEMERGAVTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRVTG
                     KLYVVEAQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            4539..6107
                     /locus_tag="ACFM4Z_001264"
     CDS             4539..6107
                     /locus_tag="ACFM4Z_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HFC6021297.1"
                     /translation="MAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEECDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSVQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     NGTTATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPHADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPVNRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            6094..7389
                     /locus_tag="ACFM4Z_001265"
     CDS             6094..7389
                     /locus_tag="ACFM4Z_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFC6021298.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLSNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTADNLVETHKIYN
                     KKGDTYLTLAYKAPDGSLYTTDRGFDYNAGRMNYRPDLDKYDRALAHQFTKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLDIDGKPDKEQKIKIRNALSRQLKFAAGVLSKETQELAGM
                     TRATVWLSDDTLVKQVDSREGQNFDDSYYAFLPDMLQNPEHVIRDNRELIFTARYKGS
                     ALWAVLKYIKEVDEIYLQSYRISNDKEIAKFMAKKKVLK"
     gene            7499..7915
                     /locus_tag="ACFM4Z_001266"
     CDS             7499..7915
                     /locus_tag="ACFM4Z_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFC6021299.1"
                     /translation="MIDVKIDNIFVVLNQIERLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            8146..9207
                     /locus_tag="ACFM4Z_001267"
     CDS             8146..9207
                     /locus_tag="ACFM4Z_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFC6021300.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFDLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTAKQEADKGNELITAALTSGKLL
                     PAQKEWAKGVLKQPGGLAFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
BASE COUNT         2468 a         2187 c         2555 g         2093 t
ORIGIN      
        1 gcttggcggt gtcgcaccat caaggctaca agtcggcatt tgcgaagcag caggcggtca
       61 tcgacaagat ggagcgcgac aaggcgcaag ccctgctgtt gtcggctcaa aactatgcgc
      121 gcgaactgga acaggcgcgt gcggaagcga aaaaatatga agtcaaggcg cacgctgtcg
      181 gcatggcttt ggcgaaaaaa caggcggaag tcagccgtct gaaaacggaa aataaaaagg
      241 aaatcgaaaa tgtccttact caagaccgta aaaatgcaag cggcggttgc attgacggct
      301 ttggctctca cggcctgcag ctctacaacc gcgccctcgg ctacggaaat taaggttgtc
      361 gaaaaggcag tcatgccgac cccgcctgct gcattgatgg tcgcgccggt acgcccgaat
      421 ccgccgaaag acggcaagac ggccacgctg ttggaacacg ccgctgagtt tggtggctat
      481 gtggcggagt tggaaaatca aaatcaggct tggcgcgact gggcgggcaa tcactcccgc
      541 aaagtcggaa actgacaaaa aagcccgcgt agggcgcggg ctgagggtga aagcggattt
      601 tatacctctt ttacaggggt agcggcggta gtgcttttca gcaaatcgac tgcgtgctga
      661 cagttttgct tgctggtgta gccttcgccc tgagcgatga tttcatggtt ggctgctttc
      721 aaacgccaac ggtattcgcc ttttgcgtct ttatagattt caaaatacat aaggtttctc
      781 ctatgaatga gtacacgttt tcttaccgct ttaacggcaa gtcctggtca ttgagcattt
      841 gggcggacaa ccctgaagaa gccagggcga aatttcgggc tgcacgagaa aatgcgcact
      901 atgacggcga agttgtagca aaggtttata catttgtaaa tatttcgtgg gttaagaaat
      961 tgtacaagcg gacaaaatat ttaatgggta tcaaagaatg acctaccgtg aattagttga
     1021 acgtcagttg gctgtgcgcc atgccgattt ggaattgggc ttaagccgcg cacgcgagca
     1081 agagccgttt gtcattcatg tttccgatct gttggataag gcaggcattg agtacgcggt
     1141 acgcatggat aaggattttc agacgacgtt tcaccttgaa tatccaatta cgaactatga
     1201 cacctttaaa cgtgcggttt ggcaaacttt gggggcgtat tactgtgttt gtaatgatgg
     1261 tgatggactg gagattgcca gcaatcgccc tgacggttac gccgtccgta tcgtattcgg
     1321 cgatgtgccg gtttaaaggg gttttaaatg gactttgaat ttggtttcag aaccctgtgg
     1381 ccgattgcga cggcggcatt ttggttttgg gtcaacggca tttcaggccg cctgaaagag
     1441 gcggacaagc gtatcgacga ccttaaagag gagttgcacg cggtcaagct ctcttatcac
     1501 accaaggcgg acgccaaggc agacagcact aatattgcgg cggccttgga gcgaattgaa
     1561 aacaagttag aaaaagtaaa cgaaaaactg gacaggaaag cagacaaatc atgagcgacc
     1621 cgattttgga tgccttggcg cgtattgaaa acaagactga tcaaacgctg aaaaatcaga
     1681 aggaaatgca ggcggaaatt gcgcaaattc gccaagacac gaaacgcacg gccattacat
     1741 tcggcgcact gggcggcggc gtgattacgg tcggctggga attgcttaaa gcgaaaatgg
     1801 gactgtaatt atggctcacc cgcaagaaat ccgtgaaaag ttacgccggc tctatgtgag
     1861 cggcgagcaa actttggaaa cggcggcctt gatgtgcgaa atcccgcaga ccactgcgcg
     1921 tgcgtggaaa cgtgcggata aggaaaaagg cgacgactgg gataagatgc gcgccgctta
     1981 cactttggcc ggcggcggta ttgaggattt gagccgtgcg atgttggccg gttttatggt
     2041 gcagtacaac agcacgatga cgatgctgca ggattcgagt accgaagatt tgccaccatc
     2101 cgaccgcgcc aagctgttgg ccagcctggc cgatgcgttt acgaaaaccg tatcggccaa
     2161 tgcgcgtgtg atgccggaaa cgtcaaaact ggcgacggct ttggaattga ttgagttctt
     2221 gatggcgttt gtacaagaaa aacatcccaa acatttgcct gcctttgtgg aggtattgga
     2281 gccgtttggg gtggaagtgg agaagaagtt tggttagagg ccaacaaatt tttttaaaag
     2341 agtaatgagg gtggcggcag ggatagcatt aattgcattt tccgtaagcg tacttaatgc
     2401 agtgtccttg attttcccta attctttttt caaccagctt ttttctgaat cagaaatctc
     2461 tgcttggtct atttttgccg taattaaagc ctgaatagtg tcgctgtgta gtttgactgt
     2521 gacaacacca agaatggcgg ataggccgcc atcatcggta aggaagtcta tgcccttatg
     2581 attaattttg cagtcaaaat ttttatgaag tgaatctatc gaagtaattt caattaaacc
     2641 agattcttct aagtaataaa tattttttaa aaaatattgg aattcatctg attgtaaagt
     2701 tgctaggtgt tgactttgaa tttcacaacc aagagtcaaa gccaagccca atccttgttt
     2761 atcaacaggg atgttagaag taataggtaa agaactacta gggaaaagag agttatacac
     2821 cttggttgct tttaggcagt tcgggtaatt atcacttaaa actcgtaaga ttttttcctg
     2881 aatacctcta tttaaccagt tcataaatta ttcctcatga aaacaaaaga attcctcaaa
     2941 tcccttgccg aactggccgc cagtttgcgc caagtcatcg aagcggaagt ggacggcttc
     3001 gatgcgtcgc ccaaggctat tgctgcacgc cgtgccaagg tgtttgaccc ggtaggcggt
     3061 tacgagtatt tcgtgaatac ctacttcccc cattatatcc gctcgcctga gaaatccgaa
     3121 ctgcatgcgt ttttattcag ccgtctgccg gagattatcc gctcccccaa aggggaaaat
     3181 gaggcggtgg gtgcgccgcg tggagagggt aagtcgacga aggttactca gttgtttacg
     3241 ctgtggtgta ttgtgaccgg ccaaaaacat tatgctgtta ttgtgatgga cagtatcgac
     3301 caggcatatc cgatgctgga agccatcaag gcggaacttg aatttaaccc gcgcttgaaa
     3361 accgactttc cggaagtatg cgggcagggc cgtgtatggc aggccggtac gattgtgacg
     3421 gccaatgacg ttaaagtcca agtggccggt agcggtaaaa agctgcgcgg tttgcgtcac
     3481 ggcccttacc gtcctgactt aactgttttg gacgatattg agaatgacga gcaagtccgc
     3541 aaccccgaac agcgcgacaa gctcaatgcg tggctgacta agaccgtatt gcctctgggc
     3601 ggtgtcggtc agaaatacga tgtgatttat atcggcacga ttttgcatta cgacagcgta
     3661 cttaaccgca ctttgaataa cccgttttgg cacggtatta agtttaaggc gatgaaacgc
     3721 tggcctgacc gcatggattt gtgggacagg tgggaggaac ttttccgaaa cgacggcgag
     3781 acggtggccg aggcgtttta tcaggcaaac aaagacgaga tggagcgcgg cgcggtcact
     3841 tcttgggcgg cgcgtggcgt actcgcgctg atgaaaatcc gtgcgcgtga cggccatgcg
     3901 acgtttgatt cagaatatca aaacgatccg gtcagtggcg aagatgcgcc gtttgccaag
     3961 tcgatgaagt tttggaacga cctgccgtcc gatttggtgt atttcggtgc gctcgacccg
     4021 tcactcggaa aggccggggc gagccgtgac ccgtccgcga ttatcattgg cggttatcaa
     4081 cgtgtaaccg gcaaactgta tgtcgtggag gctcagatta aaaaacgtct ccctgatttg
     4141 attattgagg acgttattcg attgcaccgt caatatcgtt gcaaactgtg gtttgttgag
     4201 actgttcaat ttcaggaatt tctgaaagac gagctggtca agcgcagcgc ggcgcgtgga
     4261 atacctgtcc cagcgcgggc ggtcaaaccg gtatcggaca agctgttgcg gatcgagact
     4321 ttacagcctc acatggcgaa cggtttgatt ctgttgaatg agagccaaca aacgctgata
     4381 cagcagttcc gccattttcc aaaggctgat catgatgatg gtcctgatgc cgtgcatatg
     4441 ctctggtcgg gggcggtggc caattgtgtg ccgatagaat ggcaaagccc taccgataac
     4501 gattttgatg acgagataaa aagtaaatgg agccgataat ggcaaaaaag aacaataaaa
     4561 ctaaaatcca aaagcccgaa gctgcattgc agacggacgt ggctcaaatt acggcgaccg
     4621 gtcgggttat cgccgagcat ccgtccaatt ttattacgcc gcaaaagatg cgggccctct
     4681 tcgaggacgc agaaagcggc gacatccgcg cccaacacga gcttttcgcg gacattgagg
     4741 agtgcgacag cgacatcgcg gcaaatatgg ggacgcgcaa acgcgcgctg ctgacgctca
     4801 actggcgcgt cgccccgccg cgaaatgcga cgcccgaaga agaaaagctg tccgaccaag
     4861 cctacgaaat gatggacagc ctgcctaccc tcgaagacct gattatggat ttgatggacg
     4921 cggtagggca cggattttct gcgttggagg tcgagtgggt attttcagac ggcctttacc
     4981 taccccgaaa ctttatccac cgcccgcaaa gctggttcaa atgggacaaa gacaacgggc
     5041 tgctgctgcg tacccgcgaa aatccggaag gcgaagcgtt gtggccgctg ggctgggtcg
     5101 ttcataccca aaaatcgcgc agcgtccagc aggcgcgcaa cgggcttttc cgcacgcttt
     5161 cctggctgta tatgttcaaa cactacgccg tccacgattt tgccgagttt ttggagctgt
     5221 acggcatgcc catccgtatc ggcaaatacg gcgcgggcgc aactaaagag gaaaaaaaca
     5281 ccctgcttcg agcggtggcg gaaatcggtc acaacgcggc aggcatcatg ccagaaggta
     5341 tggaaataga gctccacaac gcggcaaacg gtacgacggc aaccagcaat ccgtttttgc
     5401 agatggccga ctggtgcgaa aaatcggcgg cgcggctgat tttggggcaa acgctgacca
     5461 gcggtgcgga cggaaaatcc agcaccaacg cgctgggcaa tatccacaac gaggtacgcc
     5521 gcgatttgct ggtgtcggac gcaaaacagg tggcgcaaac catcacaagc caaatcatcg
     5581 gaccgttcct gcaaatcaac tatccccatg ccgacccaaa ccgcgtgccg aaatttgaat
     5641 ttgacacgcg cgagccgaaa gacatcgcgg tctttgccga cgctatcccg aaactggtgg
     5701 atgtcggcgt acaaatcccc gaaagctggg tgcgcgacaa actggtcatt ccagatgtgc
     5761 aggagggtga ggctgtgttg gtgcggcagg taccggacaa tccggtaaac agaactgcat
     5821 tggcggcttt atccgcccac accgtaccat ctaaggctac gggcaggcat caggaaatat
     5881 tggacggcgc gttggatgac gcgctggttg agcccgattt caattctcag ctcaacccga
     5941 tggtgcgtca ggcggttgcc gcacttaatg cttgcaacag ctacgaggag gcagatgccg
     6001 cactgaatgc gctttatccg aatttggaca acgcgaaact gcgtacctat atgcagcagg
     6061 ccttgtttat cagcgatatt ttgggacaag accatgcccg cgcctgattt gggatttgcc
     6121 ttaagtctgc cgccaaaaaa ggcaatcgag tggctggaaa gtaaaaaggt tacggcggag
     6181 agctaccgca atctgacagc ctccgaaatt gccaaagtct atacgattgc ccgcatgacc
     6241 gacttggata tgctcaacga catcaaaact tcgatggttg aatcggcaaa aagtggacag
     6301 tcgtttgacg attggcgaaa aggtatcttg aatctgctca gcaacaaggg ctggctgcat
     6361 ccgaacgggc ataacggtaa ggatatcatc gacccagcca ccggcgaggt attcggttcg
     6421 ccgcggaggt tggagacgat ttaccgtacc aatatgcaaa ctgcctacaa cgccggtcaa
     6481 tatcaaggat atatggcaaa tattgatgca cgaccttatt ggatgtatga cgcggtaggc
     6541 gacagccgca cccgtccggc gcattcggca atagacgggc tggtgtaccg ctacgacgac
     6601 ccgttttggg caacgtttta cccgcccaac ggctacaact gccgctgctc ggtcatcgcg
     6661 ctgtcggagc gggatgtgga acgccagggg cggattgttg ggcaaagcac ggcggacaat
     6721 ctggtcgaga cccataaaat ctacaacaaa aaaggcgata cttatctgac ccttgcctat
     6781 aaagcaccgg atggcagtct gtacacgacc gatcgaggat ttgattacaa cgccggacga
     6841 atgaactacc gccccgattt agacaagtac gaccgtgcgt tggcgcatca atttaccaaa
     6901 gcggaaatgg gtggtgcgga ttttaaaacc agctttaaac agcttgaaaa agagttttat
     6961 gaagtcaagc aacgtttgga tattgatggc aagcccgata aagagcagaa aatcaaaatc
     7021 cgaaatgcgc tatcaagaca gcttaaattt gctgcgggtg tattgagcaa ggaaacgcaa
     7081 gaattggcag gtatgacacg agcgacggtg tggctgtctg atgatacgtt ggttaaacag
     7141 gtagacagcc gtgaggggca gaatttcgat gactcctact atgctttttt gccggatatg
     7201 ctgcaaaacc ctgaacatgt catccgcgac aatcgtgaat tgattttcac agctcgctat
     7261 aaaggctcgg cattgtgggc agttttaaaa tatattaagg aggtggatga gatttatcta
     7321 cagtcgtacc gaatcagtaa cgacaaagag attgccaaat ttatggcgaa gaagaaagta
     7381 ttgaaataga cgttgggcaa ggctcgaaat cacttgcaca cgctctcgga cgccctaacg
     7441 ggcaggctgc ggtatcgagg ttatcaccgc ttttccaacg tctgtacgga ataataccat
     7501 gattgatgtc aaaatagaca atatctttgt cgtcctaaac caaatcgagc ggcttggcaa
     7561 cgggatcgaa aaccgctacc tgctgatgcg ccgactgtcc gaaaccatgc acacggcggt
     7621 caagctcaat ttccgctacg caggccgtcc gaaatggttg gggctaaaat accgcgacgg
     7681 caagccgctt tcggattcgg gtcgtctgaa agacagtttt tccacactgt cagacaacga
     7741 tacagccctt gtcggtacga atatcgtcta tgccgccatc cacaacttcg gcggtatggc
     7801 ggggcgcaac cgcaaagttc ggattccgca acgggaattt ttgacgctga cggacgacga
     7861 caaacaggct ttgatggacg atgtgcagga ttatttttcg ggtctgatac cgtgaattta
     7921 taaaaccctc aaaaacgcgc tttttagcgc gtttttttat gcgggtaata caaacccctg
     7981 cccaagatat aaaaatcaat cctagacgct tctaaaaagc ccctgaaaac gattaattgt
     8041 gtatcgcgcg gacaggtttt aaaaaaatgg cgggagggtt tgaagcacgc ctactctttg
     8101 ttgttttttc aaataggcaa aatgaccgta ttgagagagg tacacatgtc caaaaatgca
     8161 caaaaaaccc tacttgccgt gtgcagtttc gaggtgcagc caaaagacgg gcgaatccaa
     8221 ctgctgccat atggcgaatt tcgcgcagta gacggtcgtc cgactgatgt ccctgcgtgg
     8281 tatctgaccg aagaaaacgg tcatgatgtc gcgttgttgg ccaacagctc gcgcaatcag
     8341 ttggttgtcg attatgaaca ccagacgctc tacaaagaga aaaacggaca acctgcacct
     8401 gccgccggtt ggatgcgttg gctggagttc acgcctaaag gcatgtttgc cgaagtggag
     8461 tggacggaca aggcggctgc ggcaattgcc gcaaaagagt atcgctacat ctctgctgtg
     8521 ttttcctatg acacaaaggg atatgtaagc aaaatttttc acgccgcgct gacaaatttc
     8581 cccgcgttgg acggtatgga cgaggtgctg gcggcagcgt cggcgcaaat tttaaaaccg
     8641 gaaacggagc aaaaccctat gaaagagttg ttacagcaac tgttcgacct gcctgatgcg
     8701 ggcgaagaag aactgaaggc ggcattgtcc gcgctcgtgg aagccaagcc gaaagacgtg
     8761 gcattgtctg ccgacgtgtt cgcgcagctg gcggaaaaag acagccgcat cgcggcattg
     8821 acggcgcaaa ccgccaagcc tgatttgact aaatacgcgc ctatctcagt ggttcaagag
     8881 ctgcaaagca aagtcgccgc gctgactgcc aagcaggaag cagacaaagg caacgaattg
     8941 attaccgccg cgctgacttc aggcaaattg ctgcctgctc agaaggagtg ggcaaaaggc
     9001 gtattgaaac agccgggcgg cttggcattt ttgaccggct ttattgaaaa cgcccagccg
     9061 gtcgctgcac tggcaggctc gcaaacgggc ggcaaagcac ccgacgaacg cgtcgccgca
     9121 ctgactgcgg aagaggcagc cgcagcaaaa atgctgggca tgtccggcga agaatttgta
     9181 aaaatcaaag aaagcgaagg taagtaatgg acaaatcagc gattttgacc gcaatcacgg
     9241 cagcattccg caaagaattt caaacggcct tggattcgga tttcaagtgc aacactaagg
     9301 tac
//
LOCUS       DAXHNE010000071         9259 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.071, whole genome shotgun sequence.
ACCESSION   DAXHNE010000071 DAXHNE010000000
VERSION     DAXHNE010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9259
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.071"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(44..3088)
                     /locus_tag="ACFM4Z_001268"
     CDS             complement(44..3088)
                     /locus_tag="ACFM4Z_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC6021301.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPNASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAAPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSHMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQGKGQAEAKSPDVSQGQSVSDGTAVRDACRRVSVNLKEPNKATVSAEA
                     RISRLIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIYIEEPAAPDA
                     WVVEPPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPSGFEQAQRSRIAETDHLADDVLN
                     GGWQEETAAIADDGSEGAAERSNGQYLSETEAFGHDSQAICPFEDVPSERPSCRVSDT
                     EADEGAFQSEETDAVYEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVK
                     VKVVDSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGL
                     ELPNPKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTG
                     SGKSVGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANA
                     LNWCVNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDNPEPLEKLP
                     FIVVVVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANI
                     PTRIAFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRV
                     VEYLKQFGEPDYVDDILSGGGSEELPGIGRSGDDETDPMYDEAVSVVLKTRKASISGV
                     QRALRIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3253..3933
                     /locus_tag="ACFM4Z_001269"
     CDS             3253..3933
                     /locus_tag="ACFM4Z_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HFC6021302.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALAVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            3962..4321
                     /gene="crcB"
                     /locus_tag="ACFM4Z_001270"
     CDS             3962..4321
                     /gene="crcB"
                     /locus_tag="ACFM4Z_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFC6021303.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPASLSPATGNLFANWIGA
                     FLIGIFAETVNHPQWKLLLITGFLGSLTTLSGFSLETVTLLQLNRPASALSNIFLHTV
                     GSLLLTWLGLKIGAAVK"
     gene            complement(4988..5524)
                     /locus_tag="ACFM4Z_001271"
     CDS             complement(4988..5524)
                     /locus_tag="ACFM4Z_001271"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFC6021304.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDEGKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYTAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(5558..5914)
                     /gene="folB"
                     /locus_tag="ACFM4Z_001272"
     CDS             complement(5558..5914)
                     /gene="folB"
                     /locus_tag="ACFM4Z_001272"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFC6021305.1"
                     /translation="MDKIFLHGIKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            5970..6572
                     /gene="plsY"
                     /locus_tag="ACFM4Z_001273"
     CDS             5970..6572
                     /gene="plsY"
                     /locus_tag="ACFM4Z_001273"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFC6021306.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPVAASFF
                     MPHVSWVWATVAIALLVLFRHKSNIVKLLEGRESKIGGSR"
     gene            6649..7494
                     /locus_tag="ACFM4Z_001274"
     CDS             6649..7494
                     /locus_tag="ACFM4Z_001274"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HFC6021307.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKELISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            7740..8714
                     /locus_tag="ACFM4Z_001275"
     CDS             7740..8714
                     /locus_tag="ACFM4Z_001275"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFC6021308.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDTFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPDGELKTESFLQAGRHQLAAGYVLYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKENPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     TGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPAKPGKLRLMYEANPMS
                     LILEQAGASASNAYQAMLDIQPENLHQRVAVFMGSSEEVAYLDRLHAK"
     gene            complement(8857..9075)
                     /locus_tag="ACFM4Z_001276"
     CDS             complement(8857..9075)
                     /locus_tag="ACFM4Z_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFC6021309.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
     gene            complement(9051..>9259)
                     /locus_tag="ACFM4Z_001277"
     CDS             complement(9051..>9259)
                     /locus_tag="ACFM4Z_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021310.1"
                     /translation="GKAENLCVKRDIVNVFINRTAVLKENFAVSDKTLDKNIIFVIIN
                     VKIQFDFLTVNQERKAICSQNTVI"
BASE COUNT         2132 a         2730 c         2154 g         2243 t
ORIGIN      
        1 ccgtctgaaa cagtcttcag acggcatttc catttgcaaa aaatcaagca ttgtccaagg
       61 ggacgagaat cgtacggttg ccgttgtgtt ccggtgcgga cacaatgcct tccgcctcca
      121 tctggtcaat cagacgcgcg gcgcggttgt agccgatacg caaggcgcgc tgtacgcccg
      181 aaatgctggc tttgcgcgtt ttcaggacaa cggatacggc ctcgtcgtac atcggatcgg
      241 tttcgtcgtc gccgctgcgc ccgatgccgg gcagctcttc gctgccgccg ccgctcaaaa
      301 tatcgtcaac atagtccggt tcgccaaact gcttcagata ttcgaccacg cggtgcacct
      361 cttcatccga ggcaaacgcg ccgtgaacgc gctgcggata ggccgtaccc ggcggcagga
      421 acagcatatc gccctgaccg agcaggtttt ccgcgcccat ttggtcgaga atcgtgcggc
      481 tgtcgatttt gctggatact tgaaacgcga tacgcgtcgg gatgtttgcc ttaatcagac
      541 ccgtgatgac atcgacgctg gggcgttgcg tggcaagaat caaatggatg cctgccgcgc
      601 gggctttttg ggcgaggcgg gcaatcagtt cttcgatttt cttgcctgcc gtcatcatca
      661 ggtcggcaaa ctcatcaacc acgaccacga taaacggcaa tttttccaaa ggttcgggat
      721 tgtcgggcgt gaggctgaac gggttgccga ttttctcccc ccttgctgcg gcttcggcga
      781 ttttttgatt gaaacccgca agattgcgca cgcccataaa gctcatcagg cggtagcgtt
      841 tttccatttc gttaacacac cagttcagcg cgtttgccgc cagcttcata tcggtaacga
      901 caggggcgag caggtgcggg atgccttcgt aaatgctcaa ttccagcatt ttcggatcga
      961 tcataatcat acgcacgtct tccggcgcgg ctttgaaaag catagacaga atcatcgcgt
     1021 tgacacccac cgatttgccc gaaccggtcg taccggcaac caataaatgc ggtgctttgc
     1081 ccaagtcggt tacgacgggc tgtccggtga tgtcctgacc gagcgcgagc gtcagcttgg
     1141 atttggattc ggcaaactcg ggcgaattga agatttcgct caggcgtatc atttggcgtt
     1201 tcgggttcgg aagttccaaa cccatacagg ttttgccggg gatggtttcg acaacgcgga
     1261 tggaagccac gccgagcgaa cgcgccaagt ctttttccag atttagaacg gaattgccgc
     1321 gcacgccgac atcgggttcg atttcataac gcgtaatcac ggggccggaa taagaatcga
     1381 caaccttgac cttgactttg aactccgcca atttttcttc gatggtgatg ctgttttcca
     1441 acagttcttc ttcggtttgc gtcgcctcgg gattgaacag cggaggcaga agcaggtcgg
     1501 ttgtcggcag gtgttcgtat accgcatcgg tttcttcaga ttggaacgcc ccttcatccg
     1561 cttccgtatc cgatacccgg caggacgggc gttcagacgg cacatcttca aacggacaaa
     1621 tcgcctgact gtcatgcccg aacgcttcgg tttccgacag atattgcccg tttgaccgct
     1681 ctgccgcacc ttcactgccg tcatctgcaa tagcggcggt ttcctcctgc caacctccat
     1741 tcaaaacatc atcagcaaga tggtcggttt cagcaatgcg gctgcgttgc gcctgctcga
     1801 atcctgacgg cggttcatag gtacggttgt agatttccga tacgggaggc ggcggcggaa
     1861 tatcgattgc gggcatggga actttcggca cttcgggtgg ttcgaccacc caagcatcgg
     1921 gcgcagcagg ttcttcaata tagatatcgg cagcttcatc ataaaccgga ccgccgtatc
     1981 ccacagacga aacggtttcc gtgaaaacat tttcggtttc agacggcatt tcgacatccc
     2041 gtttcccgac aaccgtccga ctttccggaa tcaggcgcga aatccgcgcc tccgcagaaa
     2101 ccgttgcctt gttcggttct ttcaaattga cggaaacgcg gcggcaggca tcgcggacgg
     2161 ctgtgccgtc tgaaacggac tgcccttggg aaacatccgg ggattttgcc tccgcctgcc
     2221 ctttcccctg ccctgcatga cggcggaacg gcgcattccg tgcattttcg gctttgactg
     2281 cagaaaaggc agacggatgc tgcttctcaa aatacggctc aaatccgtaa tccgcagact
     2341 cggaaaacgc ttctttgtcc gcatcgaaca tatgggaata cgtgcgttga agcacagggt
     2401 cgtccaaacc gataatctgc agcccttcca tcggggtatc ggacacgcgg actttggggg
     2461 ccgctgtttc gtttttctca aatgcatcga tatagcgttt tttcgtttcc cttaaagccg
     2521 cgctgtttaa ggcacgcgtt gcttcttcca aagtgatttc tttaaaaacc ggacggacgg
     2581 gcgaaatttc gctttcagac ggcatcaacc cttcagcaat actccggtcg aatgggatgc
     2641 ggcggttgtc gattacggca gttgcaatgt cttccgtatc ggcagcctct tcctccgcag
     2701 cttctgctgc ttccgtttcc gcttcttccg tcccgtttcc actgtctgaa tatccgtcgg
     2761 atgcgttcgg aacatccgca cggtttgcag aagcaggcgg aacaggcctg tccggttcgg
     2821 attcgtgcgc caaatagttt cggcagaaac ggacaacacc gacaaacagc caatacactg
     2881 ccgttttgac ggcatggaaa agcatcaggg caagttcggg aaaatcgggc ataccgtctt
     2941 tgatttcagg cagctccgcc tgtttttccc ctttcttttc ctgccacgca gaaacctcgc
     3001 gcatccactc gcgttcggat tgtgcgcgga caaaaaacaa gccggcaagc gcaagcaaca
     3061 aaataacgat caaaactatc caaaacatat gccgtttccc gcaagacaaa cgattaaacg
     3121 ggtttatttt aacgtgtttc catcccaaaa aaaacaaccg gtcggcaatt ctgccgaaac
     3181 tgcttcggct ttcggtttgc ccccgccgac agtataatag ccgtcctttc ccttaatctt
     3241 tgcggcattg ccatgatttt ccaaacagtc tggttttcag atatggtact gtcggtttcg
     3301 tggattgtcc ttatcctgat actggcagct tccgcgccgt ccgcattccg ctcgcttgcc
     3361 cgctaccgtt ctgcccttcc cctgtgtacc gtaatttttt ccgcagcatg gtgtctgaac
     3421 gcctctgccg gcggcggaca gcttgcccag atgaactacc acctgctcgc cgtcaatctg
     3481 gttgcactga tggtgggcac ttccgccgcc ttttggcttg ccgcgctgct gatgctgccc
     3541 tactgcctgc tgtttgccgg ttctgccggc gcatatccgc cgaacgcttt agtgctgatt
     3601 ctgcccgcgc ttgccgtcaa ccgcctgtcg cgtatgctgg tcaaccgcct gccgcccaat
     3661 attttcatct ttattttcgt caacggcttt ctcgcttccg ccgccggcat tctgctgacc
     3721 gggctggtgc tgaccggtat tttggatgcc gcaaacgctt ttccgtccga aatattgtgg
     3781 acgaccgccc tgcccgtctt tattctgctg gcgtgggcgg aagcttttct cagcggcatt
     3841 tcaactgcca tatttgttgc actgaagccc cattggatca acactttcga cgacaaccgc
     3901 tatctgaagt ccgaccgcgg catatggcgg tagccttcag acggcataga caggcaaaac
     3961 catgctttcc aacatcatcc ccctttccat cggtgcggca ctcggtgcaa cggcgcgttg
     4021 gctgctcaac ctcgccgttc ccgcatcgtt gtctcccgcc acaggcaacc tgtttgccaa
     4081 ctggataggc gcttttctta tcggaatctt tgccgaaacc gtcaaccacc cgcaatggaa
     4141 gctgctgctg attaccggtt ttctcggcag cctgaccacg ctttccggat tttcactgga
     4201 aaccgtaacc ctgctccaat tgaatcgtcc cgcttcagca ctttccaata ttttcctgca
     4261 tacggtgggt tcgctgctgc tgacttggct cgggctgaag ataggggcgg cagtcaaata
     4321 accgcaacaa attgcctccc tttgttttga cataatctat aaattagctt cgcttgttta
     4381 aatactggct gtgccaatta tagtagatta acaaaaatca ggacaaggcg acgaagccgc
     4441 agacagtaca aatagtacgg aaccgattca cttggtactt cagcacctta gagaatcgtt
     4501 ctctttgagc taaggcgagg caacgccgta ctggtttttg ttaatccact ataccgttct
     4561 caaattcaaa ttgggctttt aggtctataa caaagaaaaa gccttggtag gataaaccta
     4621 ccaaggcttt ttcttaaaaa aacgctctat taccgtgcct aaaaatgcaa aaaaagtaac
     4681 catcataacc cttttgccaa aattaatatc atctattaat tcatcatgcc atgcattaat
     4741 agatggtata taagaaagag ccaataaact aataaaccaa taacaaaaat tagtaacaaa
     4801 gatattcatt attaaccatt ttaaaaatta tcatgttcgt ttgggtaatt atcataccct
     4861 tttccctctt attactaaag cacgaatgac tttgaaggac gcgcccagag acaagccgaa
     4921 aaggcatatc tcatccgcca aacaaaatgc ccgccgatct actccggcgg gcaattccaa
     4981 catcctgtca atctttcatc agccagtatt gtaaaccgat taatgtttta ccatctttaa
     5041 tttcatcgtt tgccaatgcc tgacggactt cttctttcga catcaatacg gtttccgtaa
     5101 tctcgtcttc gtcattggca agcgtactgc ccaaacgcac gccttccgct tcgaacagat
     5161 acattttttc gttgcaaaaa ccgaccgccg tataaaaact gtaaagcagg cgcacgctgt
     5221 cggcggtata aggtgtttcc tccgccaatt ctcgcagcgc acacgctgcc atatcctcgc
     5281 ccgccacatc cagcttgccc gcaggaagtt ccaatgtcgc ctgatttgcc gcataacgcc
     5341 actgccgcac caaaaccact ttcccctcgt ccgtaaccgc caacacgcat gccgcacccg
     5401 gatggcggat gacgatacgc tgcccttcat tgccgttggg caacctgacc ttatccctgc
     5461 tgatactgac gaaaccgccc tcgtaaatgg tttcgccgcc taattttact tccctcaaat
     5521 ccacttcata tctcctttcc gtttcctatt ctgccgttca atcttcacgc ttgccgcgct
     5581 cgatttccac gccaacctcg cgcacgcctt ccaaaatacc cggcttgacg attttcacgc
     5641 gcacccacac cgcgccgaaa tatcccaaaa ccaaatcggc aatatattcc gccaacgctt
     5701 ccaaaagcag gaaatcctgt tctttcagat gtcggcgcag cgtttcgcat acctcggcat
     5761 aatgcaccgt attggcaata tcgtcgtccg aacccgcttt ctcgggaaca ccgatgtcca
     5821 aatcgacaat cagggtctgc aaccgttcgc gttcccagcc gtacacgccg ataagcgtat
     5881 ccgccttgat gccgtgcaaa aagattttat ccataatccg ccccgttttt tgctaaaata
     5941 accgcaccat tctaagtaaa gtaccgcaaa tgttcaacat accggctgtt gctgtttcct
     6001 atctgatcgg ctcactttct ttcgccgtca tcgtgtccaa gtattacggc atggacgacc
     6061 cgcgcaccta cggatcgggc aatccggggg caaccaatgt tttacgcagc ggcaaaaaaa
     6121 aggcggccgc gctgacgctc ttgggcgatg ccgccaaagg tttagttgcc gttttgcttg
     6181 cacgcgtgct tcaagaaccg ctcggtttat ccgacagcgc aatcgcggcc gtcgcactcg
     6241 ccgcgctggt cgggcatatg tggccggtgt ttttcggatt taaaggcggc aaaggcgtgg
     6301 caacggcatt gggcgtgctt ctggcactct ctcccgcaac tgccttggtc tgcgcgttga
     6361 tttggcttgt tatggcattc ggcttcaagg tgtcctccct tgccgcatta accgccacaa
     6421 tcgccgcacc ggtcgccgca tccttcttta tgccgcacgt ctcgtgggtt tgggcgaccg
     6481 tcgccattgc tttgctggtg ttgttccgcc acaaaagtaa tatcgtcaag ctgctcgaag
     6541 gcagagaaag caaaatcggc ggcagccgct gatttcccat ccatccctgt ttatgccgtc
     6601 tgaaccccgc ttcagacggc atccgaatat cccgccgttt taccgactat gctcagttac
     6661 agacacgcat tccacgccgg caaccacgcc gatatgctca aacatttcac cttgttttta
     6721 gtgctgcaat atttcaaccg caaagacaag ccctactggt acatcgacac gcacggcggc
     6781 gcgggtgtgt acaatctcga aggcagcgag gcgcagaaag tcggcgaata ccgacaaggc
     6841 atcgccctgc tccgacaggc gcaaaacctg cctgccgaac tctctgactt tgccgcgcac
     6901 atacaaaaaa tcctgccctc gcccgaactt tactgcggtt ctccgtggct ggcgcaatcg
     6961 ctgacccgcg tcggcgacaa attacgcctg tttgagctgc accccaccga ctttgtccat
     7021 ctgcaaaaca atatgggcga agcaggattg ggcaaacgcg gacaagtgtt gcgcgaagac
     7081 ggttacaaag agctgatttc cctgctgccg cccccgccgc gccgcgccac cgtcctcatc
     7141 gacccgccct atgaggaaaa acaggactac cggcgtgtaa cggaaacgct gaaagccgct
     7201 ttaaaacggt ttgagtccgg ctgttatctc atttggtatc cctgcctcag ccgcgaagaa
     7261 agccgcaagc tgcccgaaga attgaaaaaa ctcctgcccg acaactacct tcacgccgaa
     7321 cttcacgtcc acgcgccgaa agccgacggt ttcggtatgc acggcagcgg aatgttcgtc
     7381 atcaatccgc cctatctgtt ggcggaacaa ttggcggcaa acctccccgc cctgacgcgg
     7441 ctgctggcgc aagacgaagg cgcgcgctac cttttggaca gcaaaatccg ctgaccccga
     7501 tgccgtctga aacccttcag acaacatctc ccgtcaaata aacaaaaccg gacgatatac
     7561 cggcagtcgg cacttttaag aataaatatc ccctcatccg cctgccgcgt tccgacttgc
     7621 cccgcgccgt ttttttccac tacaatccca cgcgatatct ggcggaacgc accaaaggaa
     7681 cgccccgcag caatctgccg cctttcagac ggcacaaacc tttccgaaac gagcaaacta
     7741 tggacacact gacccgattc ctccccgaac acctgcaaca aaaccagctg cccgaagcac
     7801 tcggcggcgt tttactgtcc gttgtttccg cctgcaccga aatcaacgcc aaagtccgcc
     7861 tcggcgcact ggccggcgta ttgggtatgg cgggcacagg caatattcag ggcgaagacc
     7921 agaaaaaact ggatgttatc gccaacaaca ttatgattga cacactcaaa gccaaccctg
     7981 ccgttgccgg tttggcaagc gaggaagaag acactttcgt aagtgccggt gaaaacggac
     8041 gctatctcgt cctattcgac cctttagacg gatcgtccaa tattgatgtc aacatttccg
     8101 tcggtacgat tttctccatc cttgaaaaac ccgatggcga attaaaaacc gagtccttct
     8161 tgcaagccgg ccggcaccag cttgccgcag gctatgtcct ctacgggcct caaacccagc
     8221 tcgtattcac attcggacac ggcgtatata tgttcacgct taatgccgaa aacgaatttg
     8281 tgctgaccaa agaaaacccg aaagtacccg aaagtaccaa agaatttgcc atcaatatgt
     8341 ccaaccgccg ccactggctg ccccccgtcc aacaatacat cgacgagctc ttgacgggcg
     8401 aaaccggcac gcgcggcaaa aactacaata tgcgctgggt agccagcatg gttgccgaaa
     8461 tccaccgcat cctgatgcgc ggcggcgtgt tcatgtatcc gcaagacaaa cgcgaccccg
     8521 ccaagcccgg caaactgcgc ctgatgtacg aagccaaccc tatgagcctg atactcgaac
     8581 aggcgggcgc atcggcaagc aacgcatatc aagccatgct cgacatacag cccgaaaacc
     8641 tgcaccaacg cgtcgccgtc ttcatgggca gcagcgaaga agtcgcctat ctcgaccgcc
     8701 tgcacgcaaa atagacagac agccgttcac acaagtacac ggcggcggca atccgatacc
     8761 gccgccgtta tttcaaacag cacaagcctt ccggtcaaat gacaaaaatg ccgtctgaac
     8821 acatttcaga cggcatttca aaaactcaaa cccgaattat tttcccgctg ctttttgcag
     8881 gatggcgtac aactcgtctt tcagtttcaa tttggctttt ttcagctcat cgatggtttc
     8941 cgcaccgctg gtaaccggat tgttgaccag accggtaatt ttatcgtcca gctcgttgtg
     9001 ttcgtcgaac agacgggcga agcgggaatt ttcctgtttc aatttggaaa tcaaatcacg
     9061 gtattctggg aacatatcgc tttcctctct tggtttacgg ttaaaaagtc aaattgaatc
     9121 tttacattaa ttataacaaa gataatattt ttatccagtg ttttatcgga tacggcaaaa
     9181 ttttctttta acaccgccgt ccggttgata aaaacgttga caatatcacg ctttacgcat
     9241 aaattttcag cctttccta
//
LOCUS       DAXHNE010000072         9180 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.072, whole genome shotgun sequence.
ACCESSION   DAXHNE010000072 DAXHNE010000000
VERSION     DAXHNE010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9180
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.072"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..132)
                     /locus_tag="ACFM4Z_001278"
     CDS             complement(<1..132)
                     /locus_tag="ACFM4Z_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFC6021311.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     "
     gene            371..1270
                     /gene="crgA"
                     /locus_tag="ACFM4Z_001279"
     CDS             371..1270
                     /gene="crgA"
                     /locus_tag="ACFM4Z_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HFC6021312.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1499..4714)
                     /gene="carB"
                     /locus_tag="ACFM4Z_001280"
     CDS             complement(1499..4714)
                     /gene="carB"
                     /locus_tag="ACFM4Z_001280"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFC6021313.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAMNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECEARPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEQLQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(4754..5365)
                     /locus_tag="ACFM4Z_001281"
     CDS             complement(4754..5365)
                     /locus_tag="ACFM4Z_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021314.1"
                     /translation="MINIIATWAMALTYIFLLLFIIVFIPIQLYLENIKKKKFKSRII
                     DILKNNHNNLDLNDIKQMTEAVNLNNFAARKIIKQLYYTDELNLNLVRQLQKEIQQEE
                     PFDGCSDELKPTLIGINELLETHGSESQKHLLTPIISELKELNQIKHDHKKMKTQSYI
                     AYIIAIISFFIGSISFYYTITAPSGKEIANTVVEQLKANQNQQ"
     gene            complement(6213..6602)
                     /locus_tag="ACFM4Z_001282"
     CDS             complement(6213..6602)
                     /locus_tag="ACFM4Z_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFC6021315.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSEAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(7175..7300)
                     /locus_tag="ACFM4Z_001283"
                     /pseudo
     CDS             complement(7175..7300)
                     /locus_tag="ACFM4Z_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7346..8479)
                     /gene="carA"
                     /locus_tag="ACFM4Z_001284"
     CDS             complement(7346..8479)
                     /gene="carA"
                     /locus_tag="ACFM4Z_001284"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFC6021316.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWALGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAEDVLALNPDGVFLSNGPGDPEPCTYAIKAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         2018 a         2331 c         2430 g         2401 t
ORIGIN      
        1 atcaatcacg gtttcttgaa cattgtgtcc gagcgcggtc aaaacttctt tcgctttttt
       61 gtgaagcgtg cggtttaacc cgccgtgaga atgtccgaac gctttgccgc cgtctaataa
      121 taaaatattc atttgttacc tcgtttgtga attgatgaat gtattgtgcc gttttacttt
      181 ataatttaaa agtgcaaaaa taagaaaaca cttttgcgtc aaatgaaata atcagatgtt
      241 ccaacacacg ggatggcaca taaagcaccg ccctgtgtgc cgtcctgatt cggaaggggt
      301 tacacccctc ccaaataaag tctgatcctg ccgccctaaa gggcggggtt ccaaccgaaa
      361 aggaaacacg atgaaaacca attcagaaga actgaccgta tttgttcaag tggtggaaag
      421 cggcagcttc agccgtgcgg cggagcagtt ggcgatggca aattctgccg taagccgcat
      481 cgtcaaacgg ctggaggaaa agttgggtgt gaacctgctc aaccgcacca cgcggcaact
      541 cagtctgacg gaagaaggcg cgcaatattt ccgccgcgcg cagagaatcc tgcaagaaat
      601 ggcagcggcg gaaaccgaaa tgctggcagt gcacgaaata ccgcaaggcg tgttgcgcgt
      661 ggattccgcg atgccgatgg tgctgcatct gctggcgccg ctggcaacaa aattcaacga
      721 acgctatccg catatccgac tttcgctcgt ttcttccgaa ggctatatca atctgattga
      781 acgcaaagtc gatattgcct tacgggccgg agaattggac gattccgggc tgcgtgcacg
      841 ccatctgttt gacagccgct tccgcgtaat cgccagtcct gaatacctgg caaaacacgg
      901 cacgccgcaa tctacagaag agcttgccgg ccaccaatgt ttaggcttca ccgaacccgg
      961 ttctctaaat acatgggcgg ttttagatgc gcagggaaat ccctataaga tttcaccgca
     1021 ctttaccgcc agcagcggtg aaatcttacg ctcgttgtgc ctttcaggtt gcggtattgc
     1081 ttgcttatca gattttttgg ttgacaacga catcgctgaa ggaaagttaa ttcccctgct
     1141 cgccgaacaa acctccgata aaacacaccc ctttaatgct gtttattaca gcgataaagc
     1201 cgtcaatctc cgcttacgcg tatttttgga ttttttagtg gaggaactgg gaaacaatct
     1261 ctgtggataa ttctataaaa acctgccata aaattgtttt tactcactta ataactttga
     1321 gaaaaaataa ataagatgat gtatagccgc ggcaatggca gtctatatgg ggagcgtgat
     1381 agggctgttc cttatgtacg gcttgtgtgt ggcaacatcg gctttccaaa tgacaaaatg
     1441 ccgtctgaaa ccgtaaaaaa aaaaggtttc agacggcatt ttcaacctga ttcaggcatc
     1501 agttgcggtt tttcaaacgc ccgtgcagtt cttgaacgct gtacacgccc aagtggtcgc
     1561 ggcttttcgc gccttcgctc atcgcttcgc cgccggcggt ggtggtgtat tgcggcacgc
     1621 gttgctgcaa tgcgctttgg cggatgatgt ggctgtcgga cacggattgc ggatcgctgg
     1681 aaacggtatt cacgaccagt gcgatttcac cgtttttcag cgcgtcgccg atgtgcgggc
     1741 ggccttcggg gactttattg atggtttgga caatcagccc gtgttcggtc aggtattgcg
     1801 ccgtgccgcg cgtggcgcag atgccgtagc ctaaggcttg gaagtttttg gcggttttaa
     1861 tgacgcgttc tttgtcttct tcgcgcacgg agaggaagat tttgccggtc gggttgaggc
     1921 gttcgcctgc gccgagttgg gctttgtagt aggcttcgcc gaagcttgcg cccacgccca
     1981 tgacttcgcc ggtggagcgc atttccggac ccaaaatcgt atccacgccc gggaatttga
     2041 tgaaggggaa cacggcttct ttaacggcat agaaatcagg gacgacttct ttttccacgc
     2101 cttgttcttt cagggaaatg cctgccatac agcgtgcgcc gactttggcg agcggcacgc
     2161 cggtggcttt ggagacgaag gggacggtgc ggctggcgcg cgggttcact tccaatacga
     2221 acactacgcc gtcctgcacg gcaaactgca cgttcatcag tccgaccacg cccagcgcgt
     2281 acgccatcgc tttggtttgg cggcggattt cgtcttggat ttcttcgctt aaggagtagg
     2341 gcggcagcga gcagccggag tcgccggagt ggatgcccgc ctgttcgacg tgctgcatga
     2401 tgccgccgat aaccacatct ttgccgtctg aaacgcagtc cacatcgact tcaatcgcgt
     2461 tgttcaggaa gaagtcgagc aacacggggc tgtcttcgga aacctgcacg gcttcgcgca
     2521 tatatttttg caactgctcg gcagagtgga caatctgcat cgcgcgtccg cccaaaacat
     2581 aagacgggcg cacgaccagc ggataaccga tttcttcggc tttgacgagt gcttcttctt
     2641 cgttgtgggc gatgcggttg ggcggctggc gcaggcctaa gtcgttcaac actttttgga
     2701 agcgttcgcg gtcttcggcg gcatcgatgc tgtcggcgga cgtgccgatg atgttcacgc
     2761 cgttttcaac cagcgcgttg gcgagtttga gcggggtttg accgccgtaa tgcacaatca
     2821 cgccccacgg gttttcggtg cggacgattt ccaacacgtc ttccaatgtc agcggctcga
     2881 aatagaggcg gtcgctggtg tcgaagtcgg tggacacggt ttctgggttg cagttgacca
     2941 taatcgtttc aaaaccggat tcgcgcaggg cgagcgcggc gtgaacgcag cagtagtcaa
     3001 actcgatgcc ctgaccgatg cggttcgggc cgccgccgag aatcatcact tttttgcggt
     3061 cggaaggacg cgcttcgcat tcctcttcgt aagtggaata gaggtaggcg gtttcggtgg
     3121 cgaactcggc ggcacaggtg tcgacgcgtt tgtaaaccgg atgcagcttc agcgcgtagc
     3181 ggtgttcgcg aacttctttt tcgcttacgt tcaacaattg tgccaaacgt ttgtcggaga
     3241 agcctttgcg tttcagacgg cgtagggcgg cgaaatccaa atcactcaaa atgccgtctg
     3301 aaaccgcttt ttcttccttc atcaagtctt cgatttgcgc caggaaccaa gggtcgatgg
     3361 cgcagatttc gtggatttct tccagcgtga agccggcgcg gaacgcgtct gccacaaaca
     3421 gcatacgctc agggccgggg ttcgccagtt cgcggcggat ttccgctttg tcggagcttc
     3481 tcggattgaa gccgcacaag ccggtttcca agccgcgcaa ggctttttgg aagctttcct
     3541 gaatcgtgcg gcccatcgcc atcacttcac ccaccgattt catctgtgtg gtcaggcggt
     3601 cgtctgcggc agggaatttt tcaaacgcga aacgcgggat tttggttacg acatagtcga
     3661 tggacggctc gaacgacgcg ggggttttgc cgccggtgat gtcgttgcgc aactcgtcca
     3721 gcgtaaagcc gaccgccagc ttggccgcca ccttcgcaat cgggaagccc gtcgctttgg
     3781 aagccagcgc ggacgaacgg ctcacgcgcg ggttcatctc aatcacaatc atctcgccgt
     3841 tttcagggtt catcgcaaac tgcacgtttg agccgcccgt gtccacgccg atttcgcgca
     3901 ataccgccaa cgaagcgttg cgcatgattt ggtattcctt gtccgtcagc gtttgcgccg
     3961 gcgcgaccgt aatcgagtcg cccgtatgca cgcccatcgg gtcgaagttt tcaatcgagc
     4021 agatgatgat gcagttgtcg ttcttgtcgc gcactacttc catctcgtac tctttccagc
     4081 cgagaacgga ctgctcaatc agcagctcgt gcgtgggcga ggcatcgaaa ccgcgttcgc
     4141 aaatcgccaa aaactcgtct ttattgtagg caatgccgcc gcccgaaccg cccatcgtga
     4201 aagaaggacg aatcagggta gggaagccga cctgttcctg cgccgccaaa gcttcgttca
     4261 ttgtgtggca gacaaaagat ttcgggcaag acaaaccgat tttttccatc gcctctttaa
     4321 agcggccgcg gtcttctgct ttgtcgatgg cgtcttccgt cgcgccgatt aattcaacat
     4381 tgtatttcgc cagcacgccg ttgcgcgcca aatccagcgc acagttcagc gcagtttgac
     4441 cgcccatggt aggcagaatc gcatcgggcc gctctttggc aataattttt tccaccgtct
     4501 gccacataat cggctcgatg taggtaacat ccgccatttc ggggtcggtc ataatcgtgg
     4561 cggggttgga attcaccaaa atgactttat agccttcttc acgcaaggct ttgcaggcct
     4621 gtgcgcccga atagtcaaat tcgcaggcct gaccgataac gatagggccg gcgccgatga
     4681 taaggatgga ttttaggtcg gtacgtttgg gcatgatggg tctttctatg aaatcaaatg
     4741 tttagctaaa aatttattgc tgattttgat tggcttttaa ttgctctacg acagtattgg
     4801 caatctcttt tcctgatggg gctgtaattg tgtaataaaa gctaatactg cctatgaaga
     4861 aactgataat ggctatgata taggctatat aactttgtgt tttcattttt ttgtgatcat
     4921 gtttgatctg atttaattct tttaattcag aaataattgg agttaataaa tgtttttgac
     4981 tttcagatcc atgcgtttcc aaaagttcat ttattcctat tagtgttggt tttagttcgt
     5041 ctgagcagcc atcaaaaggt tcttcttgtt ggatttcctt ttgtaactgt ctaactaaat
     5101 ttaaatttaa ttcatcagtg taatatagtt gcttaataat ttttctggca gcaaaattat
     5161 taaggtttac tgcttctgtc atttgtttta tatcattaag atctaggtta ttgtgattat
     5221 tttttaatat atcaattatt ctagatttaa atttcttttt ctttatattc tctaaatata
     5281 gctgaattgg aataaaaaca attataaaga gcaaaagaaa tatatatgtt agtgccatag
     5341 cccatgtggc aattatattt atcatttatc aatatcttcc atttggttta tatattgtct
     5401 cataataagc aagccgtagc tcgcatgcag ggtgtgtgcg gtacgcacgc gttctccatt
     5461 ttccctgcaa ccccaaccca ccgtgtgcgt gccttgcggc acacacccta ccgatgggtt
     5521 caaaggtcgt ctgaaaaaac gctcgcggcg tttgttccat ctgccttgcg ataaaggctg
     5581 cctgaaaaat tcccgctgtt gatatttcgg gcattctttc tcaaaccgcc tgcaaccgca
     5641 aaatccgctg caacctgccc cctcccccgt gggggagggt tggggagagg gcattccccg
     5701 aatttcggca acctttccca acaactcaac caccgaaata caagccttgt ggcttgttgc
     5761 cctctctcta gctctctccc acgggagaga ggacggagtg gctgtagggc taagggttct
     5821 gcaaggaaac aggttgtgca ggttgctttt tgcttttcag acgacctgaa atcaacggta
     5881 aatccaagtc gcttttcaaa ccgcctgcaa ccgcaaaatc tgctgaaacc tactccctcc
     5941 cccgtggggg agggctgggg agagggcatt ctccgaattt cggcaacctt tcctaacact
     6001 ctaaccgcca agacacaagc cttgcggctt gttgccctct ctctagctct ctcccacggg
     6061 agagaggatg gggtggctgt tggggctaag gtttctgtaa actaatcagg ttatgcaggc
     6121 tgctttttgt ttttcagacg gcctttataa atgctcaatt cagtcggctt tatcctatta
     6181 ttttcaataa tccaatcaaa atcagccatc tgctatcgtg cagcttgctt ttccaattct
     6241 tgcaacacga gcaggatttc tgtcagcaca tcgtttgtct gctgtaaaat ctcgtgattc
     6301 caaaaccgca gtacggtaaa gcccaagctg ttgagatatg ccgtccgcgc gtggtcgtat
     6361 gcggcttgtt ctgtgtgctg ccctccgtct gcttcgacaa tcaacttggg cgttacgcac
     6421 ataaaatcaa caatatagtt tcccatcggc tgctggcggc ggaatttata gccgttcaga
     6481 cggcctgccc gcaggtgctg ccacaatttt gcttccgcct cgctcatttc ttggcgcatg
     6541 gctttggcgc gttggcgcaa gatggggttg tcggtggtca agagtttctc gtgcgggttc
     6601 attttgttgt cgtgctgttt gctgccctct ctctaactct ctcccacggg gagagaggac
     6661 ggggcggttg ttggggttaa aggggctgta aggaaaacaa gtcgttcagg ccacttttta
     6721 cttttcagac gacctgcaat caacggttgt aaatccaagt tgcttttcaa accgcctgta
     6781 accgcaaaac ttgctgcaac ctaccccctc ccccgtgggg gagggctggg gagagggcat
     6841 tctccgaatc gcggcaatct ttccctaatt ccctaactgc ctcaatacaa gcctttcggc
     6901 ttgttgccct ctctctaacg ctttcccacg ggagagagga cggtgcggct gtagtgatta
     6961 agggttttgc aaggaaaaca aggaaaacaa gttgtacagg ctgtttttta tttttcaagc
     7021 gacctgaaat caacgcttgt aaatccaagt cgcttttcaa actgcctgca accgcaaaat
     7081 ctgctgcaac ctaccccctc ccccgtgggg gagggctggg gagagggcat tttccaagtt
     7141 gcggcaatct ttcccaaatc ccttagtctt ctaaaataca agccttacgg ttgttgccaa
     7201 atgaaacttc ttccataccc aaaacttgcg tgtaaaacgc aatggttcgg tcaatgtcgg
     7261 caacggtcag taccagatgg tcgagtgcgc taatcttcat ttcccgcttt ctttttcagg
     7321 cggcggcagt gccgtctgaa agccattatg cccgttttgc cgctttcata ttgccaatga
     7381 atttgtcaaa caaatagccg acatcttgcg gaccggggct ggcttcgggg tggccttgga
     7441 agcagaacac aggtttgtcg gtcagctcga tgccttgcaa agtgttgtca aacaaggatt
     7501 tgtgggtaat gcgtgcgtta gcgggcaggg tgtcggcatc aacggcaaaa ccgtggtttt
     7561 ggctggtgat gacgactttg ccgctgtcca aatcttgcac agggtggttc gcaccgtggt
     7621 ggctgaagcg cattttcagg gttttcgcgc cgatggcgag gctgatgagc tggtgtccca
     7681 agcaaatgcc gaaaatcggt ttgccgcttt ccatcagttt ttgtacggct ttgatggcgt
     7741 aggtgcaagg ctcggggtcg ccggggccgt tggataggaa tacgccgtca gggttgagtg
     7801 ccaacacgtc ttccgcgctc gtttgggcgg ggacgacggt caggcggcag ccgcgcgagg
     7861 cgagcatacg caggatgttg gttttcacgc cgaaatcgta ggcgacgacg tggtaaggct
     7921 gttcgtcagg ggtaacgaaa cccttgccca atgcccattc gccttccgtc cattcgtaag
     7981 tttccgtgca ggaaacttct tttgccaagt ctttgccgac catactgccg aacgcggcga
     8041 tgagttcttg cgctttttcg attgtggcat ccgcaccggt cagaatcgcg ccgccttgcg
     8101 cgcctttttc gcgcaacagc gtggtcaggc ggcgggtatc gatgtcggcg atggcgacgg
     8161 ttttgttgcg taccagatag tcgtgcaggc tttcggaggc gcggaagttg ctgtgcaaga
     8221 gcggcaggtc gcggataatc aggccggcgg cataaacgct gcggctttct tcatcttcgg
     8281 cgttggtgcc ggtgttgccg atgtgtgggt aggtgagggt aacgatttgt ttgcagtagg
     8341 acgggtcggt caggatttcc tgatagccgg tcatcgaagt attgaacacg acttcgccgg
     8401 aagtcgaacc ttcgtaaccg attgatgtgc cgtgaaatac gctgccgtca gcgaggacga
     8461 ggagggcggg ggtgctcatg atgggaatcc tgtttttaat aaaattcttg acaatgccgt
     8521 ctgaagggac ttcggcaggg ggcatccggt cggaaaaatc cggtcaaaaa aaaacacgcc
     8581 gcgcaagata gacgcgtaac gtgcttttcg ggcaagtgcc tataccctga aagcagcata
     8641 ttttaagacg aaacataccc gatttcaagc gcaaacgcgg caggcgggct gatatgttcg
     8701 cgcgaaaacg gaaatccgtg gcgtaaaacc gcccgctccg cgcccactat gcccgttacg
     8761 catttcccca cgtccgtccg gcgaaactat gggaataccc gaatcgtcag gaatacctga
     8821 atcgtcagga atacccgaac cgtcattccc gcgcaggcgg gaatccggac ttgtcggcac
     8881 ggaaacttat cgggtacaac ggtttcttga gattttacgt cctagattcc cgcctgcgcg
     8941 ggaatgacgc gtttcatttg ccgcccctcc cgaaaaacgc aaaaaatgcc gtccgaaaac
     9001 ctttcggacg gcattttcgc gtgcaaatca gtagaagact tcacgccagc tgattcgttt
     9061 cataccgcac gtcgggccgg taatgtccaa gctgtccaaa tcgttcatca gcagggtgcg
     9121 cacacctttt ccggagaagc accggttatt gcgggcgggt tttttaccct ctttgaatgt
//
LOCUS       DAXHNE010000073         9038 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.073, whole genome shotgun sequence.
ACCESSION   DAXHNE010000073 DAXHNE010000000
VERSION     DAXHNE010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9038
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.073"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(37..762)
                     /gene="aat"
                     /locus_tag="ACFM4Z_001285"
     CDS             complement(37..762)
                     /gene="aat"
                     /locus_tag="ACFM4Z_001285"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFC6021317.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSGDLDTGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDESGETRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNTVPLKEEIGRREVACKGL"
     gene            complement(832..1266)
                     /gene="fur"
                     /locus_tag="ACFM4Z_001286"
     CDS             complement(832..1266)
                     /gene="fur"
                     /locus_tag="ACFM4Z_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFC6021318.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            1485..1862
                     /locus_tag="ACFM4Z_001287"
     CDS             1485..1862
                     /locus_tag="ACFM4Z_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC6021319.1"
                     /translation="MNKTLILALSALLGLAACSAERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDVLQNAAEALKDRQNTDKP"
     gene            1872..2681
                     /gene="dapB"
                     /locus_tag="ACFM4Z_001288"
     CDS             1872..2681
                     /gene="dapB"
                     /locus_tag="ACFM4Z_001288"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFC6021320.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDTPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2795..3205)
                     /locus_tag="ACFM4Z_001289"
     CDS             complement(2795..3205)
                     /locus_tag="ACFM4Z_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021321.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPTPNYTDWVKIKFKQFSYLKFIYGYATKSQDKDIDNILELGELKQDDEILDYGGT
                     LELIGGRCDLLTGFSIDIVCREIELEFLDQKSFN"
     gene            complement(3207..3452)
                     /locus_tag="ACFM4Z_001290"
     CDS             complement(3207..3452)
                     /locus_tag="ACFM4Z_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021322.1"
                     /translation="MDYLLEAARITKEGGEIVINGTSNNKYLRGIPNEGELARVGLRL
                     KYNGPLKEEFKQLKFHKSSRTNLDSKNLKLIVFEKVK"
     gene            complement(3460..4632)
                     /gene="lpxB"
                     /locus_tag="ACFM4Z_001291"
     CDS             complement(3460..4632)
                     /gene="lpxB"
                     /locus_tag="ACFM4Z_001291"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFC6021323.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARF
                     VGIGGELMKAEGFESLYDQERLAVRGFAEVVKRLPEILRIRRGLVRDLLSLKPDVFVG
                     IDAPDFNLGVAERLKRAGIPTLHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARKTLGVDAGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAARFLLPAATAATKRRLAEILQRSEFAGLPLTVTDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQHDAVPEKLAAALADWYEHPDKVAALQQDFRVLHLLLKKDTADLAARAVLEEAGC"
     gene            complement(4680..5672)
                     /locus_tag="ACFM4Z_001292"
     CDS             complement(4680..5672)
                     /locus_tag="ACFM4Z_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC6021324.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAERKMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPPDLAQFAVMLENGTKM"
     gene            6166..8925
                     /locus_tag="ACFM4Z_001293"
     CDS             6166..8925
                     /locus_tag="ACFM4Z_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HFC6021325.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAGRADEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRSE
                     RGQTAPETATVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEVATVPAEEMIQV
                     ETRQG"
BASE COUNT         2266 a         2905 c         2130 g         1737 t
ORIGIN      
        1 caccatattt gaacctgacc gaagcataag ccgccatcaa agccccttgc acgccacttc
       61 gcgccgcccg atttcctctt tcaacggcac ggtgttcaac atccgcagac gttcggcaaa
      121 atccgcaaac ggcagcagct ccgaaccgaa acggcgcata tgttccgtat cctgctggca
      181 gtctatcagt tccacgccca aatccgccaa aaacggcacg gcgcaggcaa acgcgatttt
      241 cgacgcatcc ggttgcaacg caaacattga ttcgccataa aacaccctgc cgatttgcac
      301 gccgtaaaag ccgcccgcca acctcgtttc accgctttca tcgggataat ggcactcgaa
      361 cgaatgcgcg tgtcccattt cgtgcagctt caaatatgcc gtctgaaact cgggcgcaat
      421 ccaagttccg tcctgattcg ggcgcgccgc tgccgcacaa tgcgcgacca cttccgcaaa
      481 acagccgttg accgcaaccc gatagctgcc gttgcgcagc gttttcgcca gcgagcgcgg
      541 aacgtgcaga cgttcgggaa caatcaccgc acgcggaccg accgcatacc aaaaaaacca
      601 accgtcccga gaaaaccacg gaaacacgcc gttccgatac gcctcaagca gccgccccgt
      661 atccaaatcg ccgctcacgc cgaccagccc gtcgcaccgg gccaaagcat aggcaggatc
      721 gggaaaggca taattgtcag gggcaagcag cggaatacgc atggcagttc tccaaaaaca
      781 cagacggcat ccaaaatcat gaatgccgtc tgaacaacaa accgtccgga tttaacgttt
      841 gcccttggcc tgacagtcgc tgcacacgcc gtacatataa agcgcgtgat cgacgatgcg
      901 gtagccgttt tcttccgcga ttttgtcttg cagggcttcg atttcgggat tgtggaattc
      961 cgttacctcg ccgcacttca cgcagacgat atggtcgtgg tggtcgccct tgtccaactc
     1021 ataaaccgcc ttgcccgttt caaaatggtg gcgttgcaga atacccgcct gctcaaactg
     1081 ggtcagcaca cggtaaatcg tcgccacacc gatttccaca ccctcttcca acaaaatgcg
     1141 gtacacatct tccgcactca aatgctcttc cgcatgcgtc tcgaacaaat ccaaaatctt
     1201 caaacgcggg ccggtaacct tcagaccgct gtctttcagt tgtgcaatat tgttgaattt
     1261 ttccataata ttcaataccc ctgtaaaaca atagacgtta taatacgcaa tttcggcctg
     1321 tttgcccact atcgcaccat agcagtttgc aatagtaaaa ccgcaccggc ggccgatgcg
     1381 cggacagggc ggataccgct taatcgtatg attatcgttc gcttttataa aatattcaag
     1441 cagtgttaca ctacccatcc cgatttgcac agaaaggcat ttccgtgaac aaaaccctca
     1501 tcctcgccct ttccgccctc ctcggccttg ccgcgtgcag tgccgaacgc gtttcactgt
     1561 tcccctcgta caaactcaaa atcatacagg gcaacgaact cgaaccgcgc gccgttgccg
     1621 ccctccgccc cggcatgacc aaagaccaag tcctgctcct gctcggcagc cccatactgc
     1681 gcgacgcatt ccataccgac cgctgggact ataccttcaa cacctcccgc aacggcatca
     1741 tcaaagaacg cagcaatctg accgtctatt ttgaaaacgg cgtactcgtc cgcaccgaag
     1801 gcgacgtcct gcaaaacgct gccgaagccc tcaaagaccg ccaaaacaca gacaaaccat
     1861 aaggaacaca catgacaccg ctcaaaatcg ccatcgccgg cgcaaacggc cgtatgggac
     1921 gcgtattggt tgaagccgtc aacaaccatc ccgacaccgt cctttccggc gcgctcgaac
     1981 actcgggctc agaagccctc gggctggacg caggctacgc cgtcggactc aaaaccggca
     2041 tcgccatttc agacgacgtt gacgccgttc tcgcacaaag cgacgtactc atcgacttca
     2101 cccgccccga gcccaccctc aaacacctgc aaaaatgcgt tgaaaaacaa gtcaacatca
     2161 tcatcggcac aacaggcttc gacgatgcgg gcaaagccgc tatccacact gccgccgaac
     2221 aaacaggcat cgttttcgcc gccaacttca gcgtcggcgt caacctcacc ttccacatcc
     2281 tcgacaccgt cgcacgcgta ctaaacgaag gctacgacat cgaaatcatt gaaggccacc
     2341 accgccacaa agtcgatacc cccagcggca ccgcgttacg catgggtgaa gtcatcgccg
     2401 gcgcgctcgg ccgcgacctc aaacaatgcg ccgtttacgg ccgtgaaggc cacaccggtc
     2461 cgcgcgatcc gtccaccatc ggctttgcca ccgtccgcgc aggcgacatc gtcggcgacc
     2521 acaccgccct cttcgccacc gacggcgaac gcgtggaaat cacccacaaa gccggcagcc
     2581 gcatgacctt tgccgccggt gccgtccgcg ccgcagtttg ggtaaacggc aaaacgggtt
     2641 tgtacgatat gcaggacgta ctcgggctga acaaccgtta acccccatac aaaatgccgt
     2701 ctgaaaagat attgttcaga cggcattttg ccaacaggct ccgtatcggc atatcaataa
     2761 agttgcctgc gcagcatgac aagctacggc tcgtttaatt gaaactcttc tgatctaaaa
     2821 attctaactc tatttcccgg caaactatat ctatactaaa accagttaga agatcacatc
     2881 taccgcctat caattccagc gtacctccat aatccaagat ttcatcatcc tgctttaatt
     2941 cgccaagctc caatatatta tcgatatctt tatcttggct ctttgtagcg tatccataga
     3001 taaatttcag atagctgaac tgcttgaatt taatttttac ccagtctgta taatttggtg
     3061 taggaatctc ttgaggaaag tccggataat ccacattctg gacgatgata tggaattcat
     3121 cgtttttcaa agtatagttt aatagagtgc cctcatatat atacagaaat tgataatctt
     3181 tttttggaat ggtataagtt gtcatattat tttacctttt caaaaacaat gagtttaaga
     3241 ttttttgagt ccaagttggt tctactactc ttatgaaatt tcagttgttt aaactcttcc
     3301 tttaacggcc cattatattt taaccttaat cccactcgag ccagttctcc ttcatttgga
     3361 attcctctta aatacttatt gtttgatgtg ccattaatca caatttctcc cccttctttt
     3421 gtaatacgtg ctgcttcaag aagataatcc attaaccgct caacatcccg cctcttccaa
     3481 aaccgcgcgc gcggccaaat ccgccgtatc ttttttcaac agcaggtgca acaccctgaa
     3541 atcctgttgc agcgcggcaa ccttatcggg gtgttcgtac cagtccgcca acgccgccgc
     3601 cagtttttcc ggtacggcat catgttgaag cagctccggc acagcctcct tacccaacag
     3661 gatattcggc aggccaacat gcggcacttt gattttgcgt ttcacataag cataagtcag
     3721 cggcgaaatc ttgtagctga tgaccatcgg acgcttgcac agcgccactt ccaaagtcgc
     3781 cgtaccgctc gttaccagca ccgcatccgc cgccctacac accgtttcag actgtctgtc
     3841 ggttaccgtc agcggtaatc cggcaaactc cgaacgctgc aaaatttccg ccaaacgccg
     3901 cttcgtcgcc gccgttgcgg caggcagcag gaagcgtgcg gcaggatagc gttccaacaa
     3961 caataatgcc gtctgaaaaa acaccggcgc catatagtcg atttcgctga cgcggctgcc
     4021 gggcagcagg gcgaatacgg ggatgccggc atccacgccc aaagttttcc gcgccgtttc
     4081 acggtcgtct tccaagggca taagctgcgc catcggatga ccgacaaact ccgcacgtcc
     4141 gcccgcatcg agataaagct gcggctccat cgggaacagg cacagcacgc ggttgacctg
     4201 atgcacgatt ttgcccacgc gttcccgccg ccacgcccac accgacggac tgacataatg
     4261 cagggtcgga atgcccgccc gtttgagccg ctccgccaca cccagattaa aatcgggcgc
     4321 atcgatgccg acaaacacat caggtttcaa cgacagcaaa tcccgtacca gccccctgcg
     4381 tatccgtaaa atttccggca ggcgttttac cacctcggca aagccgcgca ccgccagtcg
     4441 ctcctgatca taaaggctct cgaaaccttc cgccttcatc agttcgccgc cgataccgac
     4501 aaaacgcgcc tgcgggcaac gctttcggat ggcgcgtatc aggtgcgccc ccaacaggtc
     4561 gcccgacgct tcgccgacac tgacggcaat caaagggctt tttttatcaa ccatattcgt
     4621 ctgtccccac atactttcac gtcccgatgc cgtctgaagg cttcagacgg catcagggtt
     4681 cacattttcg tcccgttttc caacatcacc gcaaactgcg ccaagtccgg cggcagctcc
     4741 gccttcaaca ccagcggctc acccgtgagc ggatggttca agtgcagctc ggacgcgtgc
     4801 aaaaacatcc gcttcaaacc caacttctgc aaacgacggt tcgcctgata atcgccgtag
     4861 cgttcgtcgc ccgcaatcgg acagccttga gattgcaggt ggacgcggat ttggtgcgtg
     4921 cgccccgttt tcaacgtcgc ccgcaccaaa gtcaggtgcg acagcccgac accgtgcaaa
     4981 atgccgtctg aaaaacggct taacacacgg aacaccgtat gcgccgactg cccgtccgca
     5041 ctgacgcgca ccatcttttc gccttgtgcg ccggtatatt tgaacagggg cagtttgaca
     5101 tggaaattgt cgtccggcag tttgcccacc cccagcgcaa ggtagatttt tttggggtgg
     5161 tcgttacgga tggcttcgtg aagtttgacg agcgcgctgc gtttcttcgc caccatcaac
     5221 aagccgctcg tatccttgtc caaacgatga accaactcca aatacttcgc ctccggacgg
     5281 gcgcggcgca actgttcgat aacgccgaaa ctcacgccgc tgccgccgtg gacggcaacg
     5341 ccggacggtt tgtcgatgac caaaagcgca tcgtcttcgt aaacaacgtc aaacgcacgc
     5401 gccggtacgg cggcacgcct ttcagacggc atcttgcgct ccgccacgcg cacaggcgga
     5461 atccgcaccg tatccccctc cgcaatacgg ctgtcgggtt tgcagcgttt cttgttcaac
     5521 cgcacctcgc cggcgcggat aatgcggtgg atatggctct tgggaacacc cttgaggatt
     5581 tttatcagat agttatcaag gcgttgaccc gcctcatgtt cggcaacccc tatcaagctg
     5641 accgaatctt tgcttatttc gtgcgttttc atctataatc cgaacatccg tttcagcaaa
     5701 aagcgcgccc gccgcgcccc cctgaaacgg tttactttaa aacggaattt tatattaaaa
     5761 acgcactccg caaagcattt tccctgcgcc tgttcccagc cggcaggcgc agaacgtagt
     5821 caagtttgaa ttgattttgc cgtttcggcg cggaagtaag acggcagccg ggccccaagt
     5881 cccaaacgca aaccgcccaa aacacaggca tcacgataac aagaagaagc cggcccccat
     5941 ttttccgacc gcagcgttcg gaacgcggca gacaaaccgc cgcatcgatc tttatagcct
     6001 tctgtatccg acccttccgc acacggttct ccggaacgtg catccctgcg gattttcaac
     6061 tcaaaaactg attaccggtt ttatccgaaa acatcggaaa acccagcctt acggaatgcc
     6121 gaaccggaca ggtgcgcccg aatgccgccc gtccacgagg tgatcatgaa aagaatgtta
     6181 ttcaacgcaa cgcaggccga agagctgcgc gttgccatcg tcgacggaca aaacctgctg
     6241 gatttggata tcgaaacgct gggcaaagaa cagcgcaaag gcaatatcta caaaggcatc
     6301 attacccgca tcgagccgtc gctggaagcg tgtttcgtcg attacggaac cgaccgccac
     6361 ggcttcttgc cgtttaaaga agtctcccgc tcatacttcc aagactacga aggcggacgc
     6421 gcgcgcatcc aagacgtgct caaagaaggc atggaagtca tcgtccaagt cgaaaaagac
     6481 gagcgcggca acaaaggcgc ggcgctgacc accttcatca gcctcgccgg acgctatctg
     6541 gtattgatgc cgaacaaccc gcgcggcggc ggcgtatccc gccgtatcga aggcgaagaa
     6601 cgtcaagaac tcaaagccgc tatggcgcaa ctcgacattc cgaacggcat gagcatcatc
     6661 gcccgtaccg ccggcatcgg ccgcagcgcg gaagagttgg aatgggattt gaactatctc
     6721 aaacaactct ggcaggcgat tgaagaagca ggaaaagcgc atcacgaccc ttacctgctc
     6781 tttatggaaa gctcgctgct catccgcgcc atccgcgact atttccgccc cgacatcggc
     6841 gaaattctgg tggacaatca agaagtttac gaccaagttg ccgagttcat gagctacgtc
     6901 atgccgggca atataggccg tctgaaactc tacgaagacc acacgccgct gttttcccgc
     6961 ttccaaatcg aacaccaaat cgaaagcgcg ttttcgcgca gcgtcagcct gccctccggc
     7021 ggcgcgatcg tcatcgacca taccgaagcc ctcgtctcca tcgacgtgaa ctccgcacgc
     7081 gccactcgcg gcgcagacat tgaagacacc gcgttcaaaa ccaatatgga agccgccgaa
     7141 gaagtcgccc gacaaatgcg cctgcgcgac ttgggcggct tggtcgtcat cgacttcatc
     7201 gacatggaaa accccaaaca ccagcgcgat gtggaaaacg tcctgcgcga cgcgctcaaa
     7261 aaagaccgcg cccgcgtaca gatgggcaaa ctctcccgtt tcggactttt agaattgagc
     7321 cgccaacgtt tgaaaccggc tttgggcgaa agcagccacg tcgcctgtcc gcgctgcgcc
     7381 ggcaccggcg tgattcgggg catcgaatcc accgccctgc acgttttacg catcattcaa
     7441 gaagaagcga tgaaggacaa caccggagaa gtgcgcgcac aagtgcccgt cgatgtcgcc
     7501 accttcctgc tgaacgaaaa acgcgccgag ctgtttgcga tggaagagcg tttggatgtc
     7561 aacgtcgtcc tgattccgaa catccacctc gaaaatccgc actacgaaat caaccgcatc
     7621 cgcaccgacg acgtagaaga agacggcgaa ccgagctaca aacgcgtcgc cgagcctgaa
     7681 gaagacgaat ccgccaaacc gttcggcggc gaaaaagcca aagccgcccg tcccgaaccc
     7741 gccgtcaaag gcgtgcgcca caccagcccc gccccgactg ccgcccccga gaaaaaaacc
     7801 tcctggtggg acagcttcaa agcttggctc aaacgcattt tcggcggcag cgaaacccaa
     7861 gccgcgcccg ctgccgaaac ctccgaaaaa cgcagcacgg caaaccgcag cggcagccgc
     7921 gccaacaacc gccgccaaaa cccgcgccgc agcaaacgcg aaggcagcaa agtagaagtc
     7981 cgcgaagtgg caggcaaaac tgccggacag gaagcgcgtg ccgacaaagc cgaaacgcgc
     8041 aacaacggca accgccgccg caacgaacgc ggcgaccgtg ccgccggacg cgccgacgaa
     8101 gcggaaatcc aagaccgcaa catacagcct gccgcaaccg ttgcagatgc cgcaccgtcc
     8161 gaaaccgaag tgcaaaccgg aaaacgccgc cgcaacggca gccgcagcga acgcggccaa
     8221 accgcgccgg aaaccgccac cgttgccgaa acaaccgttc agacagcgga aaacacgccg
     8281 tccgaaccgc ataccgcaga agacaaaggc agcaagccca aatccgaacg caaccgccgc
     8341 gaacgcgaca gccgagatgc caaagaacgc cgcgagcgca acaatcagcg cgaccgccgt
     8401 caaaacggca aaaaacgcaa tattccgtct gccgccaaaa tcgagcagta cctgaatatt
     8461 cacgacaccg ccgacaaagt ccgttccgcc gccgcgcacg ttttcggcga aaccgacgca
     8521 aacgcgccga ttactgtcag cattgccgat ccggttgcag aaagggatct tccgacagca
     8581 tctcccgccg tttcaaacgg cgacgcaccg gtttatgatg cggcggaaaa aatccgccgt
     8641 gccaccgccg ccatcctgcc cgaaggcgcg acaccgaaag ccgaagcaca ggaaatgccg
     8701 tctgaaaccg caacctttac ggctgcggcg gaacaggcac gggaaaccgc acaaaccggc
     8761 ggactcgtcc tgatcgaaac cgaccctgcc gcattgaagg catgggcggc acaacccgaa
     8821 gtccaagccg gacgcggttt gcgccgttcc gaacagccca agccgtctga agtcgcaacc
     8881 gtccctgccg aagaaatgat ccaagtcgaa acccggcaag gctgaaccga cggcggcaaa
     8941 aagaggttct gttccgcaga acctcttttt tacatgggtt cggatacctg caatgccgtc
     9001 tgaaacttcg ccattcccgt gattaccgaa acattccg
//
LOCUS       DAXHNE010000074         9036 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.074, whole genome shotgun sequence.
ACCESSION   DAXHNE010000074 DAXHNE010000000
VERSION     DAXHNE010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9036
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.074"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            173..952
                     /gene="pgeF"
                     /locus_tag="ACFM4Z_001294"
     CDS             173..952
                     /gene="pgeF"
                     /locus_tag="ACFM4Z_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFC6021326.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDDPEAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAEALGGTPNADAS
                     VDDTGKAACAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            1004..1483
                     /gene="lptE"
                     /locus_tag="ACFM4Z_001295"
     CDS             1004..1483
                     /gene="lptE"
                     /locus_tag="ACFM4Z_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFC6021327.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            1485..2483
                     /gene="holA"
                     /locus_tag="ACFM4Z_001296"
     CDS             1485..2483
                     /gene="holA"
                     /locus_tag="ACFM4Z_001296"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFC6021328.1"
                     /translation="MAAHIGHIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGYTVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVPRVCRLLDGLREEGGEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEEGDAWAAFKQLAVSLAK"
     gene            2604..3023
                     /locus_tag="ACFM4Z_001297"
     CDS             2604..3023
                     /locus_tag="ACFM4Z_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021329.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            3099..3614
                     /locus_tag="ACFM4Z_001298"
     CDS             3099..3614
                     /locus_tag="ACFM4Z_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC6021330.1"
                     /translation="MMTLKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(3698..4582)
                     /locus_tag="ACFM4Z_001299"
     CDS             complement(3698..4582)
                     /locus_tag="ACFM4Z_001299"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFC6021331.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDTENSRQSLELNSDLVNQLAEINKNLR
                     KHHDLAKLGVADILRFPGVLASQRENTEELAKSITELTEKALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIDALSEFFPTLLETHKEKIRARLVEAVGDINNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIITEHKGSSGKRLDFLMQELNREANTLGSKSIAAECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(5066..5929)
                     /gene="rpoH"
                     /locus_tag="ACFM4Z_001300"
     CDS             complement(5066..5929)
                     /gene="rpoH"
                     /locus_tag="ACFM4Z_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFC6021332.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(6193..7731)
                     /gene="lnt"
                     /locus_tag="ACFM4Z_001301"
     CDS             complement(6193..7731)
                     /gene="lnt"
                     /locus_tag="ACFM4Z_001301"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFC6021333.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASNNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMDMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            complement(7800..8526)
                     /locus_tag="ACFM4Z_001302"
                     /pseudo
     CDS             complement(7800..8526)
                     /locus_tag="ACFM4Z_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            8722..>9036
                     /locus_tag="ACFM4Z_001303"
     CDS             8722..>9036
                     /locus_tag="ACFM4Z_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
                     /protein_id="HFC6021334.1"
                     /translation="MDWMGSLFLPGGALLFLSVVSTTLSARLGMPLLLVSPANVLDRA
                     AEALAIAAFLMLVARPSAVFGGLWKFNYSLREKAYSRIEMQSDTVLQAGIWRGTSCPT
                     ARS"
BASE COUNT         1990 a         2255 c         2648 g         2143 t
ORIGIN      
        1 taagggcatc attcccgcac ctgcaagaat ccgaatccga acgtttgaac aattgaaaac
       61 aatcccgaat ctccgaattc ccgcctgtgc gggaatgacg aaaaaacaaa catccatttg
      121 ccccgaaggc agttaatcaa ccctttccgc cacacaccta ttccaatatc caatgaaaac
      181 catcacagaa accctaaatc tcgccccgaa aggcaaaaac ttcctgaccg ccgattggcc
      241 cgcgcccgcc aatgtgaaaa ccctgattac cacgcgcaac ggcggcgtga gccaaggtgc
      301 gtatcagagt ttgaacctcg gtacgcacgt cggcgacgac cctgaagccg tgcgccgtaa
      361 ccgcgaaatc gtgcaacagc aggtcggatt gcccgttgcc tacctcaatc aaatccacag
      421 caccgtcgtc gtcaatgctg ccgaagcgtt gggaggcaca cccaatgcgg acgcttccgt
      481 ggacgacacg ggcaaggccg cctgtgccgt gatgaccgca gactgtctgc ccgttctatt
      541 ttgcgacagg gcgggtacgg cggttgccgc cgcacacgcg ggctggcgcg gtttggcggg
      601 cggcgtattg caaaacacca ttgctgcgat gaaggttccg cctgtcgaaa tgatggcgta
      661 tctcggcccc gccatcagtg cggatgcgtt tgaagtcgga caggatgtgt ttgatgcgtt
      721 ctgcacgccc atgcccgaag ccgccgccgc atttgaaggc ataggcggcg gcaaattcct
      781 tgccgacctt tacgcgctcg cccgcctgat tctgaagcgt gaaggcgtgg gcggcgtata
      841 tggcggcacg cattgtacgg ttttggaacg ggacactttc ttttcctacc gccgcgacgg
      901 agcgacaggg cgtatggcga gcctgatttg gctggacggc aatgccgtct gaacacgccg
      961 ctgatataat ctaccgactt tgtgcttttg agaaaggcaa gccatgaaca aactgtttct
     1021 tactgccgca gtgctgatgc tgggcgcgtg cggtttccac ctgaaaggtg cagacggcat
     1081 ttctccgccg ctgacctacc ggagctggca catcgaaggc ggacaggcat tgcagtttcc
     1141 tttggaaacc gcgctgtatc aggcttcggg cagggtggac gatgctgccg gcgcgcagat
     1201 gaccctgcgt atagacagcg tttcccaaaa caaggaaacc tacaccgtta cccgtgcggc
     1261 agttatcaac gaatatcttt taatcttgac ggttgaagcg caggtattga aacgcggcga
     1321 gccggtcggc aaaccgatga ccgtgtccgt ccgccgcgtc cttgcttatg ccgacaacga
     1381 gatcttgggc aaacaggaag aggaagcggc attgtgggcg gaaatgcggc aggatgccgc
     1441 cgaacagatt gtccgccgcc tgacctttct gaaggcggaa tgacgtggcg gcacatatcg
     1501 gacacattga tacggacgcg cctttgaaac ccctgtacgt catccacggc gaggaagaac
     1561 tgttgcgtat cgaggcattg gacgcattga gggcggcggc gaagaaacaa ggttacctca
     1621 atcgggaagt ttatacggca gacaatgcct tcgattggaa cgaactgctg caaaccgcag
     1681 gcagtgcggg tctgtttgcc gatttgaagc tgttggaact gcatatcccc aatggaaaac
     1741 ccggcaaaac cggcggcgag gcgttgcagg attttgccgc ccgcctgccg gaagatacgg
     1801 taacgctggt tttactgccc aaactggaga aaacccagct ccagtccaaa tggtttgcag
     1861 cattggcggc aaagggggaa gtgtgggaag ccaaaccggt cggcgcggcg gccttgcccc
     1921 aatggatacg cggacggctg gacaaaatcg gtttgggtat cgaggcagac gcattggcac
     1981 tgtttgccga gcgcgtggaa ggcaatttgt tggcggcgcg tcaggaaatc gacaagctcg
     2041 cgctgctgta tccgaaaggg tataccgtca atatcgatga ggcgcaaacc gccgttgcca
     2101 acgtcgcccg cttcgacgcg ttccaactgg caggcgcgtg gatgaagggc gatgtcccgc
     2161 gcgtatgcag gctcttggac ggattgcggg aagaaggcgg ggaaccggtg ctgcttctgt
     2221 gggcggttgc cgaagacgtg cggacgctga tccggcttgc cgccgccctg aagcaggggc
     2281 agagcatcca atccgtccgc aacagtctca gactttgggg cgacaagcag acgctcgcgc
     2341 cgcttgcggt caagcggatt tccgtcgtcc gcctgcttga cgcgctcaaa acctgcgccc
     2401 aaatcgaccg aatcatcaaa ggtgcggaag aaggcgatgc gtgggcggcg ttcaaacaac
     2461 tcgccgtgtc gctggcgaaa taaagcggca atccccaaaa tacgaaaata ctgtaaaata
     2521 ccgttaatcc tgaaaagtat tcaccaatcc gtccgaaaac atttcagacg gcacgaccac
     2581 ctcaataaag gaacattaac cctatggaca ataagaccaa actgcgcttg ggcggcctga
     2641 ttttactgac caccgccgtt ttaagcctca ttatcgtatt gattgtcgac tcctggccgc
     2701 ttgccatcct gcttgccgcc gtcatcgtcg ccgccgctgc gggcggcttt gtttggacat
     2761 cccgccgaca gcaacgccag tttatcgaac gtctgaaaaa attcgacatc gatcccgaaa
     2821 aaggcagaat caacgaggca aacctgcgcc gtatgtacca cagcggcgga caacaccaga
     2881 aagatgcgat taccctaatc tgcctgtcgc aaaaatgttc ggtggacgag gcgcacgcta
     2941 tgttcaaaaa acgcccgaca cgtcaggaaa tcaatcaaat ggcggcaaaa cagtcgcgcg
     3001 gtcagaaacg tccgcaccgt taaccgccgc aaggcatctt tgcataaatg ccgtctgaag
     3061 cctgttggcg tttcagacgg catattctga ttgaaaagat gatgacactg aaaaccgccc
     3121 cgctcaaacg ccgctttgcc gccatgctgt acgaaatgct gctggtcggt gcggcaacct
     3181 gtttggcagc attgtttgcc ggtattgccg ccatttttct gaatcccgtt tctatcgcgg
     3241 tttctgcatt ggtaacgagt atcctgataa tgggatcatg gtggctttat tttcgcgcca
     3301 actggcacgg tcaggggcag accttggcga tgaggacatg gaaaatcggc ttgtgcgacc
     3361 ttaacggcat acagccgtct ttgcacctgc tgcgcctgcg ctttatttgg gcgtgcatat
     3421 ttatcgtctt cattcctatg cttgcttatg ccggattacg ccacttcctc ggcattccgc
     3481 ccaagggcgc ggccggcgcg gcattgattt ggctgatttt accgtggggg ttcgcactgc
     3541 tgaatcccga tcggcagttt ctgtatgatt ttcttgcagg aacaagattg gtggcggtca
     3601 aaggaaagcc ttaagccttt ataccgcaaa ggtttcaacc tgaaaaaatg ccgtctgaaa
     3661 gggctttcag acggcatttg cttatcgggg aaaccgatta ttcgatattc tgcacctgtt
     3721 cgcgcatctg ttcgatcagg actttcagct cgaccgaggc ttgggtgcat tcggcggcaa
     3781 tggatttgct gcccaaagtg ttggcttcgc ggtttaattc ctgcatcagg aagtccagcc
     3841 gtttgccgct gctgcctttg tgttcggtaa tgatacgacg cacttcggca atgtgggtgc
     3901 gtaggcggct gaattcttcg tcgatgtcgg atttttgaat aaagagggca aattcctgtt
     3961 gcagcctgtc attattgatg tcgccgactg cttcgacgag gcgggcgcgg attttttctt
     4021 tatgtgtttc cagcagggta ggaaagaatt cgctcaatgc atctatgatt tcttccatgc
     4081 tttcgaggcg ttgtaacagg tgctcgccca attttttccc ttcgcgccgg cgtgcggcag
     4141 taaagtcttt taacgctttt tcggtcagtt cggtaatgct ttttgccaat tcctccgtat
     4201 tttccctttg gcttgccaat acgccgggga aacgcaggat gtcggcaacg cccagttttg
     4261 ccaaatcgtg atgcttgcgg aggtttttgt tgatttcggc aagctggttc accaaatccg
     4321 agttgagttc caaggactgt ctgctgtttt ccgtatcttg aatttggatt ttgcattcga
     4381 ctttgccgcg cgcgatatgg gatgaaattt tctcgcggat accgctttcc aaatagcgca
     4441 aatcatccgg catcctgatt tgaatgtcca aaaagcggtg gttgacggcg cggatgtcga
     4501 gattgatgcg tttgctgccg cactctgccg ccgcgttggc aaatccggtc atgctgtgga
     4561 tgtggatatt tccgctgctc atgtcgttct ccgaagcccg ttaaaatgga atcaatatat
     4621 cacatctgta tggcggcaag cgttttcggg tgtgaaaaat tgaagatttt gcagcggcag
     4681 attggaatca cgcgcttttg ttgctgcaag gaagggaaat gtatagtgga ttaaccaaaa
     4741 ccagtacggc gttgcctcgc cttagctcaa agagaacgat tctctaaggt gctgaagcac
     4801 caagcgaatc ggttccgtac gatttgtact gtctgcggct tcgccgcctt gtcctgattt
     4861 ttgttaatcc actatatcaa tttcgccaat ctgtcggaaa agcagctgat gcggcagtgt
     4921 ctggtgcatg tctgcttttt gatttcggcg attgcgatgg cttggacgga taaaatcgtg
     4981 tacggcacga cgcacaaacc gcattgatgt ttaccaaata aaatacccga caaaacaatt
     5041 tgtcgggtat tttattgcgt atatttcaaa ccgcttcggc ttcttcggtc aggaaaccac
     5101 gcagtttctg catggctttc gcttcaatct ggcggatgcg ctcggcagag acgccgtatt
     5161 ctgcagcaag ctggtgcagc gtcagaccgc cgtcatcttg aagccagcgg ctttccacaa
     5221 tacggcggct cctgtcatcc agttgcgcca aagcgttttg taaaccttct gtttgcaggg
     5281 cgtaatgcgc ctgtttcgat agttgtcggc tcggttcgga atcgtggtcg gcaagccagt
     5341 cgatgggggc gaaactgtcc tcgtcgtcgc tgttgtctgc catgatggcg atgtcgtgtc
     5401 ccgtcatgcg ctgttccatt tccagaactt cggaaagttt gacacccaaa tcgtcggcga
     5461 tgtcttgtgc ctctttggga gacagggcgt tgaggttttt acgcatactg cgtaaattga
     5521 aaaacagctt gcgttgcggt ttggtggtgg caacgcggac aaggcgccag ttcctcagga
     5581 tgaattcgtg aatttccgct ttaatccaat gcacggcaaa tgaaaacagg cgtgcacctc
     5641 gacctggctc gtagcgtttg accgccttca tcagtccgat attgccttcc tgaatcaggt
     5701 ctgcctgatt cagcccgtag ccgtcatagc cgcgcgcgat ggaaacgacg acgcgcaggt
     5761 gggacaggat gagttgtttg gcggcgttga ggtcgccctt tatccggcgt tcggcgaggc
     5821 gggtttcttc ttcctgggac agcatcggga tgctgttgac ggtgtggatg tattgttcga
     5881 ggctgccgtt gccgctttgg atggcgggta atgcgaaagc gttattcatt tggggcattt
     5941 cctttcggct gaaactgcgt atcggcggtt tgctgtgttg ggatgcagta tatcactgct
     6001 tggcttgtat tttgtatgtt tggcaggaga tatgcgctaa ggtttgaaag acaggaaaat
     6061 tttgtaaggc aggtttgatt gattttgtaa acctgatggc tcaatccgat ttcggaatta
     6121 tattacatac gtagttgtat gtaaacagcc ggtttgaaaa aagacagccc gtctggacgg
     6181 gctgtgcagg tatcagtgtt ctttgtttcg gaagatgaaa agaatcagtg cggctagggt
     6241 caatatgccc atcaaccacc atgaactgcc ggttttcata tagggcgttt cgccgacata
     6301 gcctttgatg tgtccttcca atacggtttc cgtatcgggt tgggcttggg cgatgatgtt
     6361 gcctttgggg gagatgatgg cggttgcgcc ggtgttggtg gcgcggacca tatagcgtcc
     6421 gagttccata gcccgcgcct gcgattgttg gaggtgctgg tacatggcgt tggattttcc
     6481 gtaccacgcc atattgctgg cattggcaag cagggtggca tcttttgcgg cggcaatcag
     6541 ttcgtcgccg aatccgtctt cgtaacagat gttgaaggcg attttttggt ttttcatcag
     6601 cagggcggat tgcttgccgc cgcctttgcg gaagtcggaa aggggcatat ccatcatttt
     6661 gtaaagcggc gtggtcagga aaggcagcgg tttgtattcg ccgaagggga cgaggtggtt
     6721 tttggcgtag taggggatac cgtcctgatt gttttcctga taaccggtca ggttgatgac
     6781 ggcgttttcg taaccgttgc cgtccgaagt gtattggctg atgccgacgg cgagcgcgct
     6841 gccgttgttt tgcgcctgtt cggcaaattt cgccagtatg ttttccggca ggttttggcg
     6901 cataacgggg atggcggttt cgggcaggat gacgatgtcg gcggtggttt tgccgacttg
     6961 ttcgtaatat ttctgtatgg tcgggataac ttggtcttca cgccatttga gggtttggtc
     7021 gatgttgcct tgaagcaggg cgacggtgct gcggctgccg tcggggcggg tgaagtcggt
     7081 ttgtcgggcg gtgtagcctg cggcaagcag ggcggcaatc aggataatcg gaagcaggcg
     7141 tttgcccgaa cgtgcggtgt tattactcgc caaaaccagc cagacaccga gaaaggcggt
     7201 tgccagtgta accatgtgga tgccgcccaa tggggcaaag ccggcgagcg ggctgtccgg
     7261 ggtgatttgg gagtagccga ttgcgcccca gccgaatccg gtcaggaaac gttcgcgggc
     7321 aaactcggtc agcgtccaca ggatgggcag taccaaaccg atttttatgc cccgaggcag
     7381 ggtaaatttt ttccacagcc agaaacacag tgccggataa agggcaaggt aggcggggag
     7441 taggaaggtc agcggtacgg catagaggtc gggcaggccg gaaacgtcgt gcagggcggt
     7501 gtgtatccag tagaactgtg tcgtgtatgc ggtcaggccg aacaggtagg cggaagagac
     7561 agcaaaacgc ggacgcagtt cgatgaggcg gacgaaggca ccgaaaatca agggcatcag
     7621 ccaaaagtgg tagtagggtg cgaaggtaaa gggggtggcg gcggcaaaaa ggatgagcaa
     7681 aggccagtag agggcggggt gctgccagta tttgtccagt ttggaaacca tattcatctg
     7741 tctgttcgga agataccgtc tgaacatctt tcaaacggca tcggtatttg aaaaaggaat
     7801 caatgcctgc cgaaacgatt catcagcggc aaggcggggg cgcaggcaat cgaaccggaa
     7861 agcagcccga caacgaggtt ggcgaagtac tccgcccagc cagcgtccca gtgttgcgcg
     7921 tgcaggaaat cgtgcagaag gccgaggttg tgggcgacca gtacgccgcc gataagaaac
     7981 atggcaagcg tgccgaccac gctcaaaccg cgcataaagc aaggcataaa ggcagtcagc
     8041 atttgcccca aactgcgcga aaaggtttgt gggcggcgca tcagcagcat gcctaagtcg
     8101 tcgagtttga cgatgacggc aacgattccg tacaccaaaa cagtcatgcc gatgccgatt
     8161 gccgccatta cgagcatgcg cgtcatgtcg gtgcagaaac ttgtgcagca gcttttctac
     8221 gccttcaaag cacagataaa tgccgcctgc cgtcaaaagc ggcgtaatga gttgcggcag
     8281 gaaggcggaa agcagcaggg ccgcaggcac caaaaccggc ttgttggaaa aagaaccttt
     8341 cgccatcgac caaacaatcg gcaactcgcg ttctgccgat acgcccgtaa cccggttggc
     8401 attgggtgcc aaatcgtcgc cgaccacgcc ggcggttttc tttgcggcgg ctttggtcat
     8461 cagggcaaca tcgtccaaaa cggcggtgat gtcgtccggc agggtaaata gtgaggcaaa
     8521 tgccattaaa gaatcctgaa atgcggcgca aagtccgaca ttatatagga gaacgcggat
     8581 ttgggcggtt tcaggcggca tgaaacagga aaatgccgtc tgaacgctgt ggcggacgtg
     8641 aagtaaagtt tcgtgaaaag aaaataccgt gttacagtct ttcgatttta atttcatgaa
     8701 ttttaaggga gaatcgttag cgtggattgg atgggcagtc tgttcctgcc gggtggcgca
     8761 ctgttgtttc tgagcgtggt ttcgaccact ttgtccgcac gtttgggaat gcctttgctg
     8821 ctggtttctc ctgccaacgt gttggacagg gcggcggaag ccttggcgat tgcggcgttc
     8881 ctgatgctgg tcgcgcgtcc gtcggcagtg ttcggcggtt tgtggaaatt caattacagc
     8941 ctgcgtgaaa aggcgtatag ccgaatagaa atgcagtccg acaccgtgct tcaggcgggg
     9001 atttggcgtg gtacatcctg cccgacggca aggtcg
//
LOCUS       DAXHNE010000075         8929 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.075, whole genome shotgun sequence.
ACCESSION   DAXHNE010000075 DAXHNE010000000
VERSION     DAXHNE010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8929
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.075"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..86)
                     /locus_tag="ACFM4Z_001304"
     CDS             complement(<1..86)
                     /locus_tag="ACFM4Z_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC6021335.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPV"
     gene            complement(497..772)
                     /locus_tag="ACFM4Z_001305"
     CDS             complement(497..772)
                     /locus_tag="ACFM4Z_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6021336.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(769..2052)
                     /locus_tag="ACFM4Z_001306"
     CDS             complement(769..2052)
                     /locus_tag="ACFM4Z_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6021337.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGPFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(2208..3626)
                     /gene="glnA"
                     /locus_tag="ACFM4Z_001307"
     CDS             complement(2208..3626)
                     /gene="glnA"
                     /locus_tag="ACFM4Z_001307"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFC6021338.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDTTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDAGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            complement(3900..4277)
                     /locus_tag="ACFM4Z_001308"
     CDS             complement(3900..4277)
                     /locus_tag="ACFM4Z_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFC6021339.1"
                     /translation="MKKMLCLAVFALGLATPAVAADWVLVDSNTATFGDADSRTDNSA
                     WFETRFAKPQKYGNGKFFNTAKTLEEMDCSGKRFRILTATWYSKSGNSIASYTPSYAE
                     WNYVIPGTLGEAQYKFVCNGYPR"
     gene            4449..5258
                     /gene="aroE"
                     /locus_tag="ACFM4Z_001309"
     CDS             4449..5258
                     /gene="aroE"
                     /locus_tag="ACFM4Z_001309"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFC6021340.1"
                     /translation="MTALPRYSVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIPLKNGKLRGD
                     NTDGLGLVGDIVKVQHTEIEGKNILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELSRLFGIEAVPMADLNGGFDIIINGTSGGLNGQIPDIPPDIFQNCALAYDMVYGCA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFTPDIRPVIEYMKAL"
     gene            5261..5962
                     /gene="mtgA"
                     /locus_tag="ACFM4Z_001310"
     CDS             5261..5962
                     /gene="mtgA"
                     /locus_tag="ACFM4Z_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFC6021341.1"
                     /translation="MFRIIKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWMPYKRISTNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LKRMGSAELPESDTD"
     gene            complement(6165..6240)
                     /locus_tag="ACFM4Z_001311"
     tRNA            complement(6165..6240)
                     /locus_tag="ACFM4Z_001311"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6205..6207),aa:Phe,seq:gaa)
     gene            complement(6431..7165)
                     /gene="lptB"
                     /locus_tag="ACFM4Z_001312"
     CDS             complement(6431..7165)
                     /gene="lptB"
                     /locus_tag="ACFM4Z_001312"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFC6021342.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVIGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVTLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRSPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGTV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(7239..7769)
                     /gene="lptA"
                     /locus_tag="ACFM4Z_001313"
     CDS             complement(7239..7769)
                     /gene="lptA"
                     /locus_tag="ACFM4Z_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HFC6021343.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVTYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVIIQPSSTQKSE"
     gene            complement(7750..8331)
                     /gene="lptC"
                     /locus_tag="ACFM4Z_001314"
     CDS             complement(7750..8331)
                     /gene="lptC"
                     /locus_tag="ACFM4Z_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFC6021344.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSSKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(8328..8864)
                     /locus_tag="ACFM4Z_001315"
     CDS             complement(8328..8864)
                     /locus_tag="ACFM4Z_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HFC6021345.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
BASE COUNT         2129 a         2382 c         2248 g         2170 t
ORIGIN      
        1 ttaacaggtt cacatgaggt catagtaaaa acactcccaa taaacaaaat aaaaagttga
       61 taaatggtag tagcatatgg tctcataatt tcaagcctag aaattagtta aagaatatat
      121 tatggtagat actaactctt taagttgaca tattgtgcga tatcgagggc tgtaccgtat
      181 tttcccatcg tattttccca tcgtattttc ccatcgtatt ttcccgttct ttatatgagc
      241 ggcaggcatc ctcataaaag aagggatacc gataaggaaa aataccccag cataaaaaat
      301 atactgatta atcaatacaa atccaacaaa cagttcatcg tattcttttc tgttggcgta
      361 taaaaatact gatatattcc ttcactttca tagggcgacg ctttatgtac cgtcctgtgt
      421 gttgaaacgt cggttttcct taccgcctgc cccgcttttc cctgattgct gccaactgcc
      481 gtccgaactt gaaccgtcag gtttcagacg gcattttttc tgcctgctcc cacaattgcc
      541 gtttttgtgc ggcggtcagt tttttgacgg cgatttcctg cctgagtgcc gtgcctttat
      601 ccatcccgcc tgcaacgata cgcatcgcca ccggtttgaa tacgcgggta tatttcgcgc
      661 ctttaccggt tgtgtgggcg gcaagccgct gttgcggatt cgggctgatg ccgcaataga
      721 acgcgctgtt ttcacacaat atcaggtaaa cgctccagtt tgacgcgttc atctgcctgc
      781 atcctgagtt ttctccccgt tccaaggcgc aactttcagc agcagcagca tacccggtag
      841 ggcgagtgcg aaacacagtt ggaaaaacgg tacataaccg agccattcaa tcagataacc
      901 ggcaaaggaa ttgatgaccg tgcgcgggac ggcggacagg ctggtaaaca gcgcaagctg
      961 cgttgcggta aatgcgggat tggtttcacg cgccatatac gatacgaacg ccgccgtccc
     1021 caaccccacg ccgaccgctt ccgcgccgat aactgccgcc agcatcagcc tctcgcctgt
     1081 gccgaccgtg tcgaaaggtc cgaaccctgc cagccataca aaccccaaaa cggttacagc
     1141 ctgcaccgcg ccaaatagcc acaaggcttt gtttacgccg attttcagca tccacacacc
     1201 gcccaagata cctgccgcca ctgccggcca cagtcctgca tttttcgcaa tcaaaccgat
     1261 gtctgtcttg ctgaaaccca tatccagata aaacggcgtt gccaacgcgg ttgccatact
     1321 gtcgccgagt ttgtaaagga agataaacag cagcacgcac accgccgaag cgatgccctt
     1381 gcgcgtaaaa aattctttaa acggctctac cacggtctgc ttcaacgttt taggaacggc
     1441 aggaggcaac acgggttcgc gcgcaagaaa cagcgtcatc agaaggccgg gcagcataaa
     1501 taatgaagtg ataacaaata cttctgacca cggcatcctg tctgccaaca ccaaactcaa
     1561 tgaaccggga atcagggcgg caatccggta ggcgttcaca tgaaccgagt tgcccaaacc
     1621 caattcttcg tctgacaaaa tctcgcgcct gaacgcatcc aatacaatat cctgactggc
     1681 ggaaaaaaaa gcgacaagca ccgacaagcc ggcaatcagc ggcagatgat tacgggggtt
     1741 taaaaaggca taggccgcca aagccgccag caaccctgcc tgcgtcagca gcatccaccc
     1801 gcgccgccgt cccaaaacgg gcagcctgac cgcgtccatc agcggcgacc acaaaaattt
     1861 ccaagtaaac ggcagaccga ttaacgccat cagcccgatg ctcttcaaat ccacctgctc
     1921 gctgcgcaac cacgccggaa tcaggttaat cagaaagtac agcggcagcc ccgaggtaaa
     1981 ccccgtaaaa atacagacaa gcatatttcg ggaaaagatt tgcccgataa aacctgattt
     2041 cgatgcagtc atcgttcgtg cagcccaaaa aaaacaaaag cctattgtag caaatatcgc
     2101 ccgcctgctg aaaaatgccg ccccgcgccg ccagtttgcc gttcaaatgc aaaatgccgt
     2161 ctgaaactgt gttcagacgg cattgctttt ctgaaaacca gacgtgctta caggctgtaa
     2221 tacatttcaa attccagcgg gtgcggcgcc atgcggatgc ggcgtacgtc ttcttctttg
     2281 aaagcgatgt agctgtcgat ccagtctttg ctgaacacgc cgccgcgcag gaggaattcg
     2341 tggtcggctt tgagggcggc cagtgcttct tccaaagaag cgcaaacggt cggcaccaat
     2401 gcatcttctt ccggcggcag atcgtacagg tttttatcgg cagggtcgcc cggatggatt
     2461 ttgttttgaa tgccgtccaa accggccatc aacagggcgg caaatgccaa atacgggttg
     2521 gcggtcggat cggggaaacg cgcttcgatg cggcgcgcct tgctgctgtt cacggacgga
     2581 atgcggatgg aagcggaacg gtttttggcg gagtatgcca gtttggtcgg cgcttcaaag
     2641 tgcggcacga ggcgtttgta ggagttggtg gacggattgg taatcgcgtt caaggctttg
     2701 gcgtgtttga tgatgccgcc gatgtagtag agcgcggtgt cgctcaagcc ggcatagccg
     2761 tcgcctgcga acaggttttg accgtctttc caaatggatt ggtgaacgtg cataccgctg
     2821 ccgttgtcgc ccataatcgg tttgggcata aaggtggctg ttttgccgaa gttgtgggca
     2881 acgttttgaa tcacatattt catgtcttgg gtttggtcgg cgcgtttgac caaggtggcg
     2941 aaacgcgtgc cgatttccat ttggctgccg gtaccgactt cactgtggtg gacttcgact
     3001 tcgatgccga gttcttccaa aatgtttacc atcgcggagc gcaggtcttg tcccgcgtca
     3061 atcggagcaa cgggcgcgta accgcctttg acggcagggc ggtggccggt gttttgaccg
     3121 tccatatgca gaccgctggc ccatgcgccg ctttcggacg tgatttcgta acgggttttg
     3181 tgcatatcgg tttcaaattc tacgccgtcg aagacgaaga attcgggttc ggggccgaag
     3241 tatgccgtgt cgccgatgcc ggaagatttc aaataggcct cggcgcggcg tgcgatggag
     3301 cgcgggtcgc ggtcgtaacc ctgaccgtcg gcagggtcga tgacgtcgca ggtaatgacg
     3361 acggtggtat cgtcgtagaa aggatcgacg aaggctgtag acgcatcggg acgcaactgc
     3421 atatctgaag cctgaatacc tttccaaccg ccgatggacg aaccgtcaaa cgcctgtccg
     3481 ttttcaaacc actcttcggg gtcttccaac acgatgcgcg caggcacggt aaagtggtgc
     3541 tgcttgcctt tggtatcggt aaagcgcaaa tcgacaaagc gggcttcgct ttcttcaatc
     3601 aattttacgg cgtttttaat agacattttt cagctcctgg aaaagggaaa tagcaaaata
     3661 tgcggaacgc ggcagattag ctcaaagcca gcatgattct accacgtcca tgccgtctga
     3721 aactggccct gcgtcaaatt ccaagctgta aaatatagtt tcagacggca atattgtcaa
     3781 caatatgctt cggtttgccc gttcccaagc agaatgcccg cccttttatc cgaatgccga
     3841 caaaacagat gccgtctgaa agcagttcag acggcatctg ttgtttggtt tgaaaacggt
     3901 cagcgaggat agccgttgca gacaaatttg tactgagcct ctccaagcgt accgggaata
     3961 acataattcc attcggcata ggatggtgta taagaagcga tagagtttcc cgatttggaa
     4021 taccacgttg ccgtcaatat cctaaaacgt ttaccactgc aatccatttc ttccaacgtt
     4081 ttagctgtat taaaaaattt tccattgcca tacttttgcg gcttggcgaa ccttgtttca
     4141 aaccatgcac tgttgtccgt acggctgtct gcatcaccaa acgtggcagt attactatct
     4201 accaataccc aatccgccgc caccgccggc gttgccaacc ccaatgcaaa tactgccaag
     4261 cacaacatct ttttcatgtc cgaccctctc gtccttgtca acaaaataaa gttaaattgt
     4321 aaaaaaaaaa cgactttttt caagagaaaa atattccgtc atgctatctg acaaccgttt
     4381 cagacggcat cgtttccatc tgtacgccgc cgttataaaa tagccttccc gtcttttgga
     4441 acacgaccat gaccgccctc ccccgttact ccgttttcgg caaccccgtt gcacacagta
     4501 aatcgccgca aatccatcag caatttgccc ttcaggaagg cgttgacatt gaatacgaac
     4561 gcatttgcgc cgacatcggc ggttttgcac aggcggtttc gacatttttt gaaacaggcg
     4621 gttgcggggc aaacgttacc gtaccgttca aacaggaagc gtttcatctg gcggacgagc
     4681 attccgaacg cgcattggcg gcaggtgcgg tcaatacgct gattccgttg aaaaacggca
     4741 agctgcgtgg cgacaacacc gacggtttgg gtttggtcgg cgatatagtg aaggtgcaac
     4801 acacagaaat tgaaggcaaa aatatcctat tgctcggcgc gggcggcgcg gtgcgcggcg
     4861 tgattccggt tttgaaagaa caccgtcctg cccgtatcgt cattgccaac cgtacccgcg
     4921 ccaaagccga ggaattgtcg cggcttttcg gcattgaagc cgtcccgatg gcggatttga
     4981 acggcggttt tgatatcatc atcaacggca cgtcgggcgg tctaaacggt cagattcccg
     5041 atattccgcc cgatattttt caaaactgcg cgcttgccta cgatatggtg tacggctgcg
     5101 cggcaaaacc gtttttagat tttgcacgac aatcgggtgc gaaaaaaact gccgacggac
     5161 tgggtatgct agtcggtcaa gcggcggctt cctacgccct ctggcgcgga tttacgcccg
     5221 atatccgccc tgttatcgaa tacatgaaag ccctataaac atgttccgca tcatcaaatg
     5281 gctgattgcc ctgcccgtcg gcatctttat ctttttcaat gcctatgtgt acggcaacat
     5341 cattacctac cgcgccgtcg cgccccatcg gactgccttt atgtcgatgc ggatgaagca
     5401 gtttgaacag gaaggtcgcg atgtcgcact ggattaccgc tggatgccct acaaacgcat
     5461 ttccaccaac ctgaaaaaag ccctgattgc ttccgaagat gcccgtttcg ccgggcacgg
     5521 cggcttcgat tggggcggca ttcaaaacgc catcaggcgc aaccggaaca gcggcaaagt
     5581 gaaggcgggc ggctcgacca tcagccagca gcttgccaaa aacctgtttt taaacgaaag
     5641 ccgcagctat atccgcaaag gcgaagaagc ggcgattacc gcgatgatgg aagccgttac
     5701 cgacaaagac aggatttttg aactgtattt aaactcaatc gaatggcact acggcgtttt
     5761 cggcgcggaa gccgcgtccc ggtattttta tcaaataccc gccgccaagc tgaccaaaca
     5821 gcaggcggca aaactgacgg cgcgcgtccc cgccccgctc tactacgccg accatccgaa
     5881 aagcaaacgg ctccgcaaca aaaccaatat cgtgctcaaa cgcatgggtt cggcagagtt
     5941 gcctgaaagc gacacggact gagtgccgcc gcaatataaa atgccgtctg aaatatcatt
     6001 cagacggcat atgctttctg cctttaccct cttctccaaa acgcaacggg gcggtcatta
     6061 tcatttttgc ccttctacat aacggtcaga cacaaaacgt gtccgacacg gacaacttcc
     6121 caaaaacggc aggcaaaaaa aataaccgct tcaaggcggt tttttggtgg ccagaggcgg
     6181 aatcgaaccg ccgacacacg gattttcaat ccgttgctct accaactgag ctatctggcc
     6241 tttgtctgtc tcgttaagag atgcgtatta aaccaaatta cgggacgaca tgcaagcata
     6301 tcgggcaata ttctttgaaa acaggtttaa gtcaatgttt taaatttgaa ttaatttatg
     6361 taaaaacaac ataaatccgt atttttgccg cctgcccgac gatattaggt cgcccgtctg
     6421 aaaaatattt tcaatatttg aagttcttac ccagataaac agaacgaacc tgttcgtttc
     6481 cgaccaaatc atcaggtttt cccgatgcca acaccgtgcc gtctgaaata atgtaggccc
     6541 gatcgcagat gctgagggtt tcgcgtacgt tgtggtcggt aatcagtacg ccgataccgc
     6601 gcgatttgag gaaaccgatg attttctgga tgtcgatgac ggcaatcgga tcgacgccgg
     6661 caaaaggttc gtccaacaaa ataaaatgcg gtttcatggc gagtacgcgg gcgatttcga
     6721 cgcgccgccg ttcgccgccg gacagcgacg gcgcggggct gcggcgtaag tgtccgatat
     6781 tgaggtcggc gagcagtttt tcgatttccc tgtcgatttg atttttatct ttggttctga
     6841 tttccaagat ggcgcggatg ttttgttcga cggtcatttt gcggaatatc gaggcttcct
     6901 gcggcaggta gccgacaccg aggcgggcgc gttcgtgtat gggcaggtgg cgcaattctt
     6961 gtccgtctag ggttacgctg cctgcgtcgg cggcgatgag tccgacaatc atgtagaagc
     7021 tggtggtttt acccgcaccg ttgggcccga gcagtccgat gacttcgccg ctttcgattt
     7081 cgagggagaa gcttttaacg acttggcgtt ttttgaaact tttttgcagg ttttgaacaa
     7141 caaggcggct gacgtttgca ctcataaata tctcttcaat atcttcggtc ggcatatgcc
     7201 gtccgaaccg ggtttatatt tcagacggca ttttgggatt attcggattt ttgcgtactc
     7261 gaaggctgga tgatgacgct gaccctgccg gatttggaag cggatttcgc accggatttt
     7321 gtactgccgc tgatggtata gacttcagtt ttggtgttgt aggtaatcac cgcaccttcg
     7381 gcgacatcgc cgccgcgctg tactttggca ttgccggtca gaacaacggt gcttcctgcg
     7441 gaggaatagg taacgttgtt tgcctgtccg cgcaccgtgc ctttgccgcc gtccaatgtc
     7501 tggctgaagc ggactggcga accttccgcc ctcacggatt cgccgccttt gccgccgcgt
     7561 gtaacattga cgcgggcggc ggaaatattg agcgtaccct gtctgatgac gacgtttccg
     7621 ctgaatgtgg tgctttggtt ggcttgatcg agcgaacctt ggtcggcctc aatctgaata
     7681 ggctgcctgc tgtcgctttg aagggcaaaa gcgggggacg ccgaaaaaaa tgctattaaa
     7741 acaaatagct tacatatctt ttgtatcata aatcgtggct ttcactttag atgagaagtt
     7801 caacatgcct gttttgtggt cgtaagtcat gccgcccgcc tgaccgtgcg atgcaccata
     7861 ttggaaactg acaggcgtat cggtttgggc atattgagat tcggtatcga cgtgcagctt
     7921 ttcggcttca actttacccg cctgccgttt gccgtcggcg gttttggtca gcacaacgtt
     7981 gtttttaaaa agaacctgtt tgttttcggt atggtaaacg gcttcatcgc tgccgacttc
     8041 gtacaacaac ctgccttctt ggaagaacac gagatgcggt gagtcgaaat gaatgtcgct
     8101 gctttcggga aactgtttcg cgcccttcga actcaaatgt tctttcaagt atccctgttc
     8161 gtcaaaccgc ctgccatcca atccgtccat tgtgtattgc ggttcgtcgg gattgagcct
     8221 gacttcttca atctcgactt cgctgatgcg tcccaaccat gccgacaggc tgcccaaggc
     8281 aaccgccaat atcaatggga acgcaattcc gtaccgccat cttactttca tttgatgtac
     8341 tcgttcaaag ccgcgcccaa agtcccttgc gcctgcataa tcaggtcgca cacttcgcgc
     8401 accgcgcccg caccgccggg gcgtttggca atataggcgg cgtgttgcag cgtaaaccaa
     8461 tgcgcgtcgg ggacggcaac cggcaatccg cagcgtacca taacgggcaa atcgaccacg
     8521 tcgtcgccga caaaggcaca ctcggcttct tccacgccga cctgagcgcg cagctcttca
     8581 tacgcggcgc gtttgtccga aatgcctttg aagtagtaat ttatgcccaa ctgtttgacg
     8641 cggatgccga cggagggcgc gtcccgacct gtgataatcg cagtctgcac gccgcttgcc
     8701 tgaagcattt tcagaccgtg tccgtccagt gtgtgaaacg atttgatttc ttcgccgtta
     8761 tcgcggataa agatgcgccc gtcggtcaaa acgccgtcca catccaggat caacagtttg
     8821 attttggcgg cgcgcgcctg taattcggga gaaattgcct gcatgggatt tccttgtttt
     8881 cagacggcat atcttaccac aaatgccgtc tgaaacggct gcaacgcct
//
LOCUS       DAXHNE010000076         8837 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.076, whole genome shotgun sequence.
ACCESSION   DAXHNE010000076 DAXHNE010000000
VERSION     DAXHNE010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8837
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.076"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..203)
                     /locus_tag="ACFM4Z_001316"
     CDS             complement(<1..203)
                     /locus_tag="ACFM4Z_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6021346.1"
                     /translation="MKITHCKLKKKVQKELIRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEGSV"
     gene            complement(436..2493)
                     /gene="ppk1"
                     /locus_tag="ACFM4Z_001317"
     CDS             complement(436..2493)
                     /gene="ppk1"
                     /locus_tag="ACFM4Z_001317"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFC6021347.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELAREGIHFYRRRNWTGAQKKWIESYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            complement(2660..3103)
                     /locus_tag="ACFM4Z_001318"
     CDS             complement(2660..3103)
                     /locus_tag="ACFM4Z_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HFC6021348.1"
                     /translation="MPFRLFLGILCGLSMVAPVYAQGQPDAVSAYIQKKKVIVNTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASSVVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            complement(3110..3625)
                     /locus_tag="ACFM4Z_001319"
     CDS             complement(3110..3625)
                     /locus_tag="ACFM4Z_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC6021349.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNIKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GTDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            3892..6624
                     /gene="leuS"
                     /locus_tag="ACFM4Z_001320"
     CDS             3892..6624
                     /gene="leuS"
                     /locus_tag="ACFM4Z_001320"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFC6021350.1"
                     /translation="MQEHYRHAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     AAAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYSLPKKQVLQRVTIGLSPEQEEAMGFGGGSGC
                     GAGYGDGSGSDSRDLPFMPNKWQAWYGVKDDFMVLINSGELDGMNYQTAFDAVAAKLQ
                     AIGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCEKCGDVPVPADQLPVVLPENVVPDG
                     MGSPLAKMPEFYETTCPCCGGAAKRETDTMDTFMESSWYFFRYMSPKFSDGMVSAEAA
                     KYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEGLVNVGEPFERLLTQGMVVCETYYR
                     ENDKGGKDWINPADVELTFDDKGRPVSAVLKADGLPVVISGTEKMSKSKNNGVDPQEL
                     INAYGADTARLFMMFAAPPEQSLEWSDSGVEGAHRFLRRLWRTVYEYLKQGEAVKAFA
                     GSQDGLSKELKDLRHKLHATTAKVSDDYGRRQQFNTAIAAVMELLNQYDKTDTGGEQG
                     RAVAQEVLETAVRLLWPIVPHICETLWSELNGAKLWEAGWPTVDEAALVKSEIEVMVQ
                     VNGKLRGKITVAADASKADLEAAALATEGAVKFMEGKPAKKIIVVPGRLVNIVV"
     gene            6854..7645
                     /locus_tag="ACFM4Z_001321"
     CDS             6854..7645
                     /locus_tag="ACFM4Z_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HFC6021351.1"
                     /translation="MNLFFDTELGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMKR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNIVPDGAYHTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPHHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQMYGFEDKLSIQ
                     FPQNNHIRDKIRQQLQILRDQNTIEFIGRGLYKKIDKLPPTSKAF"
     gene            7612..7782
                     /locus_tag="ACFM4Z_001322"
                     /pseudo
     CDS             7612..7782
                     /locus_tag="ACFM4Z_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
     gene            7775..7906
                     /locus_tag="ACFM4Z_001323"
     CDS             7775..7906
                     /locus_tag="ACFM4Z_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HFC6021352.1"
                     /translation="MRNFDKLAEEAAEHAAEGAVKFIDGKPAKKIIAVPGRLANIVV"
     gene            8008..>8837
                     /locus_tag="ACFM4Z_001324"
     CDS             8008..>8837
                     /locus_tag="ACFM4Z_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFC6021353.1"
                     /translation="MKFPSFLPSFKRILCLSAVISVLGACTVVADVYGQDSATMNAAA
                     AKDYMKTVELNKSAGNVDTTSRTARRVQAVFRRMLPYADAANNTGHRFDWKMTVFKND
                     ELNAWAMPGGKMAFYTGIVDKLKLTDDEIAAIMGHEMTHALHEHGKNKVGQQILTNTA
                     AQIGTQIILDKKPDTNPELVGLGMDILGTYGLTLPYSRSLEEEADEGGMMLMAQAGYH
                     PAAAVRVWEKMNQENDQNGFIYAITSTHPTNNARIENLKRLLPTVMPVYEQSVRNKGR
                     V"
BASE COUNT         2143 a         2235 c         2430 g         2029 t
ORIGIN      
        1 tcgacagagc cctcaaaaac ctcatcggca gccaaggcca aatgatggtt gataaccgtg
       61 cggattttac ggtagaacag tgctgccgaa ttgggatgga tacccaaaat atcggcggca
      121 gaacgggcgg taacttcgag tacaaaaaaa cggatcagtt ctttctgtac ttttttcttt
      181 aatttgcagt gtgttatctt catattccca gggtaacata tctgctaatc tagtacagcc
      241 cctaaaattt aatccactat aatgccgtct ggaatgcggc ggcttggggt tgggagcgta
      301 taggggaagt gcttgtgcag gaatggcgaa ggtgtgtttt tgactccggt ccgccctgtt
      361 tagattgctg taaaaccatg ccgtctgaaa cggaaaggtt tcagacggca tggtttttga
      421 ttggggcggt gcgggtcagc ctccgagcag tgtccacaaa tcgttttgca ggtcggcttc
      481 ggattcgccc tcggcgggcg cggcgcggat atagccgccg tcgggctgca tcagccacgc
      541 gtgcgtattg tctgccaatg ccatttccaa accttcgcgg atgacgcgtt ttttgagttc
      601 gggcgcggta atcggcgcgg cggtttcgat gcggcggaag aagttgcgcc ccatccaatc
      661 cgcgctggag ataaaggtat cgtccgcgcc gttgttgtgg aagcaataca cgcgcgcgtg
      721 ttcgagctgc ctgccgatga tggagcggac gcggatgttt tcggacaagc cttttacacc
      781 cgggcgcaag gtgcacatac cgcgtacaat caaatcgatt tgtacgcctg ccgcgcttgc
      841 ccgatacagg gcttcgatga cggtcggttc gatgagcgaa ttcatcttgg cggtaatccg
      901 cgccggtttg ccggcttttg cgtgttcggt ttcgcgtgcg atgcggtcga taaccatttt
      961 gtgcagggta aacgggcttt ggtagagttt gttcagccgc ccgggtttgc ccaagcctgt
     1021 gatttccata aacaatgtgt tcacatcggc ggtgatttgt tcgtcggcgg taatgatgcc
     1081 gaagtcggtg tagatgcgcg atgtgccttg gtggtagtta cccgtgccga ggtgggcgta
     1141 acgcttgagt acgccgtctt cgcggcggat gaccagtgcc atcttggcgt ggactttgta
     1201 gccgaacacg ccgtacacga cgtgcgcgcc cgcctcttcg agctgcttcg cccagttgac
     1261 gttgttggct tcgtcaaaac gcgccatcag ttcgacgacg acggttactt gtttgccggc
     1321 gagtgccgcc ttcatcaggg cgcggacgag ttcggaacgc gtgccggtgc ggtaaatcgt
     1381 cattttgacg gcaagcacgt cggggtcggc ggcagcttcg cgcatcattt cgacaacggg
     1441 gtcgaacgat tggtaggggt ggtgcagcag gatgggcgat tggcgcacca aatcgaatat
     1501 ggacgcggtt ttgcccaagg ctttcagtct gcccggcgtg tgtgtgggaa atttcaaatc
     1561 ggggcggttg actaggtcgg ggacggcgtt gaggcgcacg aggttgaccg ggcctttgac
     1621 ctgatagagt tcggcggcgg tcagtttgaa ttgcgcgagc agaaagtcgc ggatgtaggc
     1681 gggacaggtg tcggcgactt cgagccgcac gccgtcgccg tattcgcggt cgtgcagttc
     1741 gttttggatg gcggcgcgga ggttttgcag gtcttcttcg tcaacggtca agtcgctgtc
     1801 gcgcgtcagg cggaactggt ggcagccttt gacgttcatg cctgggaaga gtttgccgac
     1861 gtgggcgtgg aggatggacg acaggaagac gaagccgtgt ccgccgccac acagttcgga
     1921 cggcagggga acgacgcgcg gcaggatgcg cggtgcttgg acaatcgcca tacccgaagg
     1981 cctgccgaat gcgtctgtgc cgtcgagttc gacggcgaag ttgagcgatt tgttcagcgg
     2041 gcgcgggaag gggtgggaag ggtcgagtcc gatgggggtc agaatcggca gcaattcgcg
     2101 gtcgaaatag ctttcgatcc actttttctg cgcgcccgtc cagttgcggc ggcggtaaaa
     2161 atggatgcct tcccgcgcca actcgggctg aaggacgttg ttgaacaggt cgtactggtg
     2221 ccgtatcagg gagcgcgccg cttcggtaac gtcggcgatg gtttcagacg gcattttgcc
     2281 gttgtccagc ctgcgccggg ggtgcagctt gttttcacgt ttgagccacg ccatgcggac
     2341 ttcgaaaaac tcgtcaagat tggacgacac gatgcacagg aagcgcaggc gttccaaaag
     2401 ggggacgttt ttgtcttccg cctgtgccaa cactcggcgg ttgaatgcca gcaggctcag
     2461 ttcgcggcag aggatgcggt tttgttcggg cataaggttc tcctaggtgc tgtctgattg
     2521 ttctgttgga aaaggcaggt aatgccccct gaaatcttga aggtgttttt aacgtttttc
     2581 agacggcata tgcatggctt ttatcaggat ttggggcatg atgtgaaaac ggataccgct
     2641 atatacggta tccgtctgtt taaattactt ccaatacaaa ataatggcgc agtttttcgt
     2701 ataccgcgtc gctgacgttg atgcagccgt tggtcataat cctgtcggac acaaccgaag
     2761 atgcgatacg ttcgttccgc ctttccgacg gtatctgatt ccaaacgcgg tgcagggcga
     2821 aaagaaaatc gccctcctgc ttgaaaccga tgacttcgcc gccgtatccg ggcttgtcgg
     2881 tactgttcag cgtcagcccg aacgtcccct tgggcgtggc aacaccgatg aggacggggt
     2941 ggcactgcct gtcatcggca aaacagagtt ccgctttgga tgtgtttacg atgacttttt
     3001 tcttttgaat ataggcactg accgcatccg gctgcccctg tgcatatacg ggggcaacca
     3061 tcgataggcc gcataatatc cctaaaaaca ggcggaacgg catgtcggat tattggcgga
     3121 tacgtttcgg ttttgccggc acgatttcgc ggatgatgac ttgcggctcg gctttgggtg
     3181 cgggcggcgg acaggcggca tctttcggga agacggggtt ccagtagaag ctgcgggcaa
     3241 atttgtcttt atcgaaaatc actttgtatt ggcaggtagt aacatcttcc acgccggaag
     3301 tgttttcagg gtcggtacct acgccggggg tgtggaagtg aaacaggtaa tcccattcgc
     3361 gcacgccgta cataccttcg tcgtaatgcg gacgacccag gattttgtag atgtcgtctt
     3421 tggtcaggcc ggggcgcatc tgatccagtt cgtcataagt cgggaatgtg ccgcgcttgt
     3481 tgtcgagcgt tacggaatag ggtttcggga aaaccggatt gtcggtcgtg ccgtcggctt
     3541 tgatgttgct tttggttgca caggcagaca gaacgcccgc cgccaatact gccaagcccg
     3601 gtttgacgat ttgtttgatt ttcatatgta gaatcctttt ttacccgatg cccgtctgcc
     3661 ttttgttcgg aagagctgga tcgggagggt tttaaacgtt tgattttggt ttgtaatatt
     3721 agcataaaaa acgtgcgtat cagtaaacgc ggtgtatttg tacggcatac ggaatgatgc
     3781 gcgtgcgaat ttacgcatcc tgccggcaat ttgccgattc gccgacatcg gcaacctgtt
     3841 ataattcctc ctttaaattc ctaacgtttt caagtgaaaa acagaatgac catgcaagaa
     3901 cattaccggc acgccgccat cgagcctgcg gcgcagaaaa aatgggacga cgcccgtatt
     3961 ttcaacgtct ccgaagacgc ttccaaaccc aaatattatt gcctctccat gttcccctat
     4021 cccagcggca agctgcacat ggggcatgtg cgcaactaca ccatcggcga tgtattgagc
     4081 cgcttcaaac ttttaaacgg cttcaacgtc atgcagccta tgggttggga cgcgttcggc
     4141 atgccggcgg aaaacgcggc gatgaaaaac aacgtcgccc ctgccgcttg gacgtacgat
     4201 aacatcgaat acatgaaaac ccagctcaaa agcctaggtt ttgcgattga ctgggcgcgc
     4261 gaaaccgcaa cctgcaaacc cgaatactac cgctgggaac aatggctgtt taccaagctg
     4321 tttgaaaaag gcatcgtgta tcgcaaaaac ggcacggtga actgggaccc tgtcgaccaa
     4381 accgtccttg ccaacgagca agtcatcgac ggacgcggct ggcgttccgg cgcattgatc
     4441 gaaaaacgcg aaatcccgat gtattacttc aaaatcacgg attacgccga agagctgctc
     4501 aacgacttgg acaagctgga acactggccg gaacaagtca aaaccatgca gcgcaactgg
     4561 atcggcaaat cgcgcggtat gaccgtgcgc ttcgccgttt cagacgacag caaacaaggc
     4621 ttggaaggcg attacgcgaa attcctgcaa gtttatacca cccgccccga cacgctgatg
     4681 ggtgcgactt atgttgccgt tgccgccgag catccgctgg cagccgccgc agccgccgac
     4741 aaacccgaat tgcaggcatt tattgccgaa tgcaaagccg gttcggttgc cgaagccgat
     4801 atggcgacga tggagaaaaa aggcgtgccg accggtcgct acgtcgtcaa cccgctcaac
     4861 ggcgacaagc tggaagtgtg gattgccaac tatgtattgt ggggctacgg cgacggcgca
     4921 gtgatggcgg ttccggcgca cgacgaacgc gattttgagt tcgccgccaa atacagcctg
     4981 ccgaaaaaac aggtcttaca acgcgtaaca atcggacttt cgccagagca agaagaagct
     5041 atgggttttg gtggcggttc cggatgtggt gctggttatg gtgatggtag tggttccgat
     5101 tcaagagatt tgccgtttat gcctaataaa tggcaagcat ggtatggagt caaagatgac
     5161 ttcatggtct taatcaactc aggtgaactg gacggcatga attatcagac agcatttgat
     5221 gctgtcgccg ctaaattaca agctatcgga gctggcgaac cgaaaaccca ataccgcctg
     5281 cgcgactggg gcatttcgcg ccaacgctac tggggctgcc cgattcccat cgtccattgc
     5341 gaaaaatgcg gcgacgtacc cgtccccgcc gaccaactgc cggtcgtttt gcctgaaaac
     5401 gtcgtacccg acggcatggg ttcgcctttg gcaaaaatgc ccgagtttta cgaaaccacc
     5461 tgcccatgct gcggcggcgc ggcgaaacgc gaaaccgaca ccatggacac cttcatggaa
     5521 tcaagctggt acttcttccg ctatatgtcg cccaagtttt cagacggcat ggtatcggca
     5581 gaagccgcga aatactgggg cgcggtcgac caatacatcg gcggtatcga acacgccatt
     5641 ttgcacctcc tgtacgcgcg tttcttcacc aaactgatgc gcgacgaagg cttggtcaat
     5701 gttggcgaac cgtttgaacg cctgctgacg caaggcatgg tcgtttgcga aacctactac
     5761 cgcgaaaacg acaaaggcgg caaagattgg atcaaccccg ccgatgtcga gctgacgttc
     5821 gacgacaaag gccgccctgt ttccgccgtc ctcaaagccg acggtctgcc cgtcgtcatc
     5881 agcggcacgg aaaaaatgtc caaatccaaa aacaacggcg tcgatccgca agagctgatt
     5941 aacgcctacg gcgcggacac cgcccgcctg ttcatgatgt tcgccgcacc gcccgaacag
     6001 tccctcgaat ggagcgacag cggcgtcgaa ggcgcacacc gcttcctgcg tcgtctgtgg
     6061 cgtaccgttt acgaatacct gaaacaaggc gaagcagtca aagcgttcgc aggcagccaa
     6121 gacggtttgt ctaaagaact caaagacctg cgccacaaac tgcacgcgac cactgccaaa
     6181 gtcagcgacg actacggccg ccgccagcag ttcaacaccg ccatcgccgc cgtgatggaa
     6241 ttgctcaacc aatacgacaa aaccgacacc ggcggcgaac aaggccgcgc cgtcgcccaa
     6301 gaagtattgg aaaccgccgt acgcctgctg tggcccatcg tgccgcacat ctgcgaaacc
     6361 ctgtggagcg aattgaacgg cgcgaaactg tgggaagcag gctggccgac agttgatgaa
     6421 gccgctttgg tcaaatccga aatcgaagtg atggttcaag tcaacggcaa actacgcggc
     6481 aaaatcaccg tcgccgccga cgcctccaaa gccgacctcg aagccgccgc actcgccacc
     6541 gaaggcgcgg tgaaattcat ggaaggcaag cccgcgaaga aaatcatcgt cgtaccgggc
     6601 agactggtga acatcgtcgt ctaaaccgct cttaaggttt agccatacgg ataaaggccg
     6661 tctgaaaact ggaaaacagg gtttcagacg gcctttttaa ggcagattgg gttgtctgca
     6721 ttacagacct caaatatagc ggattaactt taaaccagta cggcgttgcc ccgccttgcc
     6781 ctgctgtctg cggcttcgtc tccttgtcct gatttttgtt aatccactaa aacttaatct
     6841 caaaatccaa aacatgaatt tatttttcga tactgaattg ggaaagcaac aaaataaagc
     6901 aacccacaaa atccgtgtaa tgagcgaggc ttggctggaa aaaaacggct actgtccctg
     6961 ttgcggaagc aagccgatga agagatttgc caataacaaa cctgttgcag acctcttttg
     7021 cccaaattgc cacgagcaat atgaattaaa gagtaaaaat caaaaaacca taggcaacat
     7081 cgtgcctgac ggtgcatatc acaccatgtt ggagcgcatc cggtcagata ccaaccccaa
     7141 ctttttcttt cttgcatata aaaaagcgga ttactccata cggcaattgg tgcttgtacc
     7201 caaacatttc atcacgccgg atatgattat tcccagaaat aaaggcatta aaaaccggcc
     7261 gcaccacatt atgtgttcca tcaatctcgc ccctttgcct gaaagcggaa aaatattctt
     7321 aatagacgat tcccgcatta tcgaacccga aaccgttctg aaaaaatggc aatccaacct
     7381 gtttttacgc aaccaaaatg cggagcgtaa aggctggctt ttggctgtta tgaaatgtat
     7441 cgaccaactc cccgaagaat tcacattgtc gcaaatgtat ggatttgaag acaaactatc
     7501 catccaattt ccccaaaaca accatatcag agacaaaatc cgccaacagt tgcaaatttt
     7561 gcgcgaccaa aatacgatcg aattcattgg tcgcggactt tacaaaaaaa tcgacaaatt
     7621 gcccccgact tccaaggcgt tttaatttca aatcatgata ctgaattcac aagggatatt
     7681 tattcttctg ctattctgaa aacagttgaa gtacagcgca atattgccgc aaaaggcagc
     7741 agccgtaaaa aagtcgggga attattggca atttatgcgt aatttcgaca aattggctga
     7801 agaagctgcc gaacacgccg ctgaaggcgc ggtgaaattc atagacggca agcctgccaa
     7861 gaaaatcatc gccgtaccgg gcagactggc gaacatcgtg gtgtaaatct ttttaaaaac
     7921 atcaaacaat gttaagatgc cgtccgaaat ccgttttaga ttcagacggc attttttaaa
     7981 tttaattttt taaggggtaa gcctaatatg aaatttcctt ccttccttcc ttcctttaaa
     8041 cggatactct gcctgtcggc agtaatctcg gtattggggg cttgtacggt cgttgctgat
     8101 gtttacggtc aggattccgc cacaatgaac gctgcggctg ccaaagatta tatgaaaacg
     8161 gttgagttga acaagtctgc cggcaatgtc gataccacat ccagaacagc ccgcagggtg
     8221 caggcagtat ttcgacgtat gctgccttat gccgatgcgg caaataatac cggccatagg
     8281 tttgactgga aaatgacggt tttcaaaaac gatgagctga acgcgtgggc gatgcccggt
     8341 ggaaaaatgg cgttttatac ggggatagtc gacaaactca agctgaccga tgacgaaatt
     8401 gccgccatta tggggcatga aatgacgcac gccctgcatg aacacggtaa aaataaggtc
     8461 gggcaacaaa ttttgaccaa tacggcggcg cagataggca cgcagattat attagacaaa
     8521 aaaccggaca ctaatccgga attggtcgga ttgggtatgg atattttggg gacgtacggt
     8581 cttaccttgc cttatagccg cagcttggaa gaagaagccg atgagggggg aatgatgttg
     8641 atggcgcagg caggctatca tccggcggcc gctgtcaggg tttgggaaaa aatgaatcag
     8701 gaaaacgacc aaaacggctt tatttatgct attacctcta ctcatccgac aaacaatgcc
     8761 cgtatagaaa acctaaaacg gttgttgccg accgttatgc ctgtttatga gcaaagtgtc
     8821 agaaataagg ggcgcgt
//
LOCUS       DAXHNE010000077         8743 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.077, whole genome shotgun sequence.
ACCESSION   DAXHNE010000077 DAXHNE010000000
VERSION     DAXHNE010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8743
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.077"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(239..3091)
                     /gene="gcvP"
                     /locus_tag="ACFM4Z_001325"
     CDS             complement(239..3091)
                     /gene="gcvP"
                     /locus_tag="ACFM4Z_001325"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFC6021354.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDDFVGNTVPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDERVYPQTLDVMKTRAKYFGFELVVGDFAKADEGEYFGALFQYVGKDG
                     DVQDLQSVIGRLKAKGTIVAVSADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGIKVVHEVFFDTVTVDFGNK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSAYEDLVDLYRAFTGKDAFAFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTAGYRELLADMENSLKSITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAQRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIVHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHVVTDMHNASADQT
                     AVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVERGEWPKEDNPLVNAPHTASDVTGEWTHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3122..3481)
                     /locus_tag="ACFM4Z_001326"
     CDS             complement(3122..3481)
                     /locus_tag="ACFM4Z_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021355.1"
                     /translation="MTDRPSLLWIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNIVFPLPAYPLAWLVPAGFMVLGGGFPLSLPLVALLVLTCCILAHCPPLS
                     RLLCYPCPNHPMSKNSA"
     gene            3480..3704
                     /locus_tag="ACFM4Z_001327"
     CDS             3480..3704
                     /locus_tag="ACFM4Z_001327"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HFC6021356.1"
                     /translation="MPSEPAGRLFQKTPYRCNFMRLYGVGFALQTAYASRSSLVFQSV
                     HDYSGLNLNQYGVASPCRTICTVCGFVALS"
     gene            complement(3756..4397)
                     /locus_tag="ACFM4Z_001328"
     CDS             complement(3756..4397)
                     /locus_tag="ACFM4Z_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HFC6021357.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4387..4938)
                     /locus_tag="ACFM4Z_001329"
     CDS             complement(4387..4938)
                     /locus_tag="ACFM4Z_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFC6021358.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIDSDNGLFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDIDTVAAFDDTDRERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAI"
     gene            complement(4942..5460)
                     /locus_tag="ACFM4Z_001330"
     CDS             complement(4942..5460)
                     /locus_tag="ACFM4Z_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFC6021359.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDNTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5460..7121)
                     /gene="pqiB"
                     /locus_tag="ACFM4Z_001331"
     CDS             complement(5460..7121)
                     /gene="pqiB"
                     /locus_tag="ACFM4Z_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFC6021360.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTLLSAVWLVPLIALIAGGWLWIKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSSSPKLRPHTVYAGDTVIATRGGGLDDLQAKVVDLLDKFDKLPLDKTVAELN
                     GSLAELKSALKSANAALSSIDKLAGNPQTQNIPNELNQTLKELRQTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(7114..8448)
                     /locus_tag="ACFM4Z_001332"
     CDS             complement(7114..8448)
                     /locus_tag="ACFM4Z_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiA/YebS family transporter subunit"
                     /protein_id="HFC6021361.1"
                     /translation="MPPLIFATFPMKPFAENIPHSLRGNCCDEALPPHTVDCPECGCR
                     ADVPRLDSGEAAFCPRCGHKLFRVGRHPFSAPPAYAAASLILMAFAYGMTYIEVGIPG
                     AASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALR
                     LATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAEVRFGPAFYLMFALSVMLIRTSVSV
                     PQHWVYFQIGRLTGDNAVQTASEGKTCCSRCLYFRDGAESPCGVCGAELYRRRPKSLS
                     ISSAFLTAAVILYFPANILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSAS
                     ILVPVLKIAAMSVLIASARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFH
                     TYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKHD"
BASE COUNT         1960 a         2238 c         2463 g         2082 t
ORIGIN      
        1 tttgtccgtg tggaaatgac atcaaagggt gtgaataggc ttttcatctt tgaaatctgt
       61 ttgcacgatc ggatgaataa aaagtaggat gataaaaaag ctgatgtatg gatactggtt
      121 taactcattt gttttaaagt tatagtcgag atacgtgtat tggtgcaacc agtctgccgt
      181 cgacgaaaat gccgtctgaa agtgtttcag acggcatttg tctccggggt ttcaacggtc
      241 agtcttcata attttccatc ggtgggcagc tgcacacgag attgcggtcg ccgtacacgt
      301 cgtccacgcg gttgacgaag ggccaaaact tgtgttcgcg gacgaacggc aacgggaaga
      361 cggcttcttc gcgggaatac ggatgcgtcc attcgccagt tacgtcggat gcggtatgcg
      421 gagcgttcac caacgggttg tcctctttcg gccattcgcc gcgctcgact ttcaacactt
      481 cctgtttgat ttgtttcagg gcggcgatga agcggtcgag ttcggctttg ctctcgcttt
      541 cggtcggctc gatcatcagc gtgccggcaa cggggaagga gacggtcggg gcgtggaagc
      601 cgtagtccat caggcgtttg gcgatgtcgg tttcggtaat gccgctttcg gctttgagcg
      661 gacgcaagtc gacgatacat tcgtgcgcga cgcggccgtt tttgcctgta tacagaatcg
      721 gatagtcttc gctcaagcgt ttggcaacgt agttggcgtt gagcaatgcc cagcgtgttg
      781 cctgctccat gccttgtttg cccatcatgg tcaggtacat ccaagtaatc ggcaggatgg
      841 acgcagaacc gaaagctgcg gcagccacgg cagtttgatc ggcactggcg ttgtgcatat
      901 cggttaccac atggcctggt gcgaacggag ccagatgggc tttcaagcca atcggaccca
      961 tgcccggacc gccgccgccg tgagggatac agaaggtttt gtgcaggttc atgtgcaaca
     1021 cgtccgcgcc gacttcggca ggctgcatga tgccgatttg ggcgttgagg ttggcgccgt
     1081 ccatgtaaac ctgtccgccg ttttcatgga cgatgcggca gatgtcgcgg atgccttctt
     1141 cgtacacgcc gtgggtggac ggataggtaa tcatgatggc ggacaaagcg tcgcggtgtt
     1201 gctcggcttt ggctttcaaa tcgtcgatgt tgacgttgcc gtgttcgtcg gtatcgacga
     1261 cgacgacttt caaaccgagc atggcggcgg tggcggggtt ggtgccgtgg gcggatttgg
     1321 gaatcaggca gatgttgcgc tgcgcttcgc cttgcgcttc ctgatagcgg cggatggcaa
     1381 gcataccgct gtattcgccc tgcgcgccgg agttgggctg gaaggaaatt gcatcaaaac
     1441 cggtgatgga tttcaggctg ttttccatat cggcgagcaa ttcgcggtag ccggcggttt
     1501 gcgcttcagg agcgtaagga tggatgtcgg agaactccgc ccaagtaatc ggcaacattt
     1561 ccgcagtcgc gttgagtttc atggtgcagc tgcccaatga aatcatgctg cggttcatcg
     1621 ccaaatcgcg gtcttcgagt tttttcagat agcgcaacat ttcgtgttcg gtgtggtaac
     1681 ggttgaacac aggatgttgc agaatgtcat cttgacgcag caattcggcg ttcagacggc
     1741 ctttgacatc atcggcaaat gcgaacgcat ccttgccggt aaacgcgcgg tacaaatcaa
     1801 ccaaatcttc gtatgccgat gtttcatgga atgcagccgc aacttgagta tcgttgacgc
     1861 ggcgcaggtt gtaacccgac tctaaagcag cggcaaacac ttggtctgct ttctctttgt
     1921 tgccgaaatc gacggtaacg gtatcgaaga agacttcgtg aaccactttg atgccgtctg
     1981 aaaccagtgc atcggcaaag gcagaagcca gcgcgtgaat gcggttggcg atgcgtttca
     2041 cgccttcagg gccgtggtaa acggcataca taccggccaa attcgccagc aatgcctgcg
     2101 cggtacaaat attggacgtg gctttttcgc ggcggatgtg ttgctcgcgg gtggacaacg
     2161 ccatacgcaa ggcaggtttg cccgatgcgt ctttggatac accgatgata cggcccgggg
     2221 cggaacgttt gaactcgtct ttaaacgcga aataagcagc atgcggaccg ccgaagccca
     2281 tcggcacgcc gaagcgttgc gtgttgccca aagcaatatc tgcacccaat tcagccggag
     2341 acttcagcaa aaccaagctc atgatgtcgg cggaaacggc gacaatcgtg cctttggctt
     2401 tcagacggcc tataacgctt tgcaagtctt gcacgtcgcc gtctttgccg acgtattgga
     2461 acagcgcgcc gaagtattcg ccttcatccg ctttggcaaa atcgccgacc accaattcaa
     2521 agccgaaata cttggcgcgg gttttcatca cgtccaaagt ttgcggatac acgcgctcgt
     2581 ccacgaagaa acgctcggat tttaccttgc ccacgcggtg cgccatcgcc atcgcttcgg
     2641 cggcggcagt tgcctcgtcc agtaaagacg cgcccgccac agggaaaccg gtcaaatcga
     2701 tgcacacttg ttggaagttc aacaatgctt ccaaacgacc ctgcgcgatt tccgcctgat
     2761 acggcgtgta ggcggtatac cagccaggat tttccaatac gttgcgcaaa atcacattcg
     2821 gcacgcgggt cgggtaatag cctaaaccga tataggactt gttgatcacg tttttcgacg
     2881 caatgccttt caatttcgcc aatgcgtccg cctcggtcaa ggcctcgggc aaatcgagtt
     2941 cagacggcat acggatgctt tgcggcacgg tgttgccgac aaaatcgtcc atacttttct
     3001 cgccgacagc ggcaagcaac gccgcttcgt cgccgaaact caaatgccgc gcggcaaatt
     3061 cgttggggtt gaacagttcg gacagtttca tttctactcc ttcgggtttg tagggctgcc
     3121 atcaggcaga atttttagac atcggatgat tcgggcaagg gtagcacaaa agacgggata
     3181 atggcggaca atgcgccaga atgcagcagg tcaggacaag cagcgccaca agcggcagcg
     3241 ataagggaaa gccgccgccc aaaaccatga aacctgcagg caccagccaa gccaatggat
     3301 acgccggaag cggaaaaaca atattgagca gaaccaaaat cacgacaaac agcggcagac
     3361 ccgccatcag gttggcacgc ctgtatgttg cgtacgggtc ggatgcttct tcaggttcgc
     3421 aatattccat gatttcagcc tgtccgggct ttatccacag caggctcgga cggtcggtca
     3481 tgccgtctga accggcaggc cgtctttttc aaaaaacgcc ataccgctgc aacttcatgc
     3541 ggctgtatgg cgtaggcttt gcacttcaga cggcatatgc gtcccgaagc agtttggtgt
     3601 tccagtcggt tcatgattat agtggattaa atttaaacca gtacggcgtt gcctcgcctt
     3661 gccgtactat ttgtactgtt tgcggcttcg tcgccttgtc ctgatttttg ttaatccact
     3721 atatataaac aacttgggtg cggataaacc tttgtttatc taaacccctg ttttctcaaa
     3781 aattgattca aatcttcggc aaatttccga tagcctttgc cgttggcgtg gatttggtcg
     3841 gatttcagat tattatcgcc caaaatttcc gcccacgcgc cgccgaacag cggaatgccg
     3901 tattcctcgg acaaatcctc atacagcgga tgatcgctca aatgcccgaa caacgcaccc
     3961 agtgtgatgt gcggcacgcc gacgaggacg gcggggatgt tttccttctg cacggtttcg
     4021 atgattttcg cgatattggc gcgggtctgc tccttgggaa ctttgcgcag aaagtcgttg
     4081 ccgcctatgc cgacaatcac aagcttgggt ttgcgtgcca acagcgcggg caggcgcgac
     4141 agggcttggg cagatgtatc gcccgatacg ccgccgttga caatattcca acccgtcagt
     4201 ttttgcagtt gcgcggggta ggattcgcca gggtttgcgc cgtagccgaa ggtaagcgaa
     4261 tcgcccaagg caagtacggt gcttccttcg ggaattttgg cgtgggttcg ggcggatttt
     4321 ctgccgcagg cggtaagcag caacgcgcct gcgccgagga ggaaggttct tctgttcatt
     4381 ggtttttcag atggcatcga gccatttcgg gtggtatttc agggtttcac ggtaaaccgg
     4441 acagctttcg gcgtgcgcgc ctttgaggta gccggtactc atcagaaatt cgccgacgat
     4501 ttcgccgccg acgaatttga aatgtttttt aaagagttta acccattcgt ctttgcttcg
     4561 cggatggtgc gcgtcgagcc agttcttgaa cgagccgtat tcttgttgca acgcttggat
     4621 ttgccgtgca ttgaaaatgg cggcatcgat tttcaggcgg ttgcggacga tgcccgcgtc
     4681 ggcaagcagg cgttcgcggt cggtgtcgtc gaaggcggca accgtatcga tgtcgaaacc
     4741 ttcaaatgcc gtctgaaacg cctgccgctt tttcagcatc agcgtccagc ttaatcctgc
     4801 ctgattgatt tccaacacca gccgttcaaa cagtccgttg tcgctgtcaa tcggaaaacc
     4861 gtattgcgtg tcgtggtaat gtttgttcgg gttatcggtg ttttcgggaa gtgaggcggc
     4921 aaattcgcaa tagttcacgg tttactcgac catctgttgc gccgcctgtt tcagtccctg
     4981 ttcgagtgcg gcggtcatcg cggcgtagcc gtcaccctgc tgttcggttt cgatatggaa
     5041 gggtctgttc gtaccgtcgg gtaggacggc gtagccgctg atgagggttt tgcccgtgta
     5101 gctgccttgg aatgcgtcga tatagaccgt ccatttttcg gtactgccgc tgcgtgaggc
     5161 aggaacaaag atgcgtgtgt tgtccaaacg gttgaatgca ttgctcaacg ccgcttcgag
     5221 catatcgtcc aaggtgtctg cccaaacgtg gttttgtgcg gtgttgaggc ggtaggggtc
     5281 ggtttgatag accagtccgc cgcgtttgag cggttcggca agacggactt cgacggcggt
     5341 ttcgccgcct tgtgttgcag gacggatgta gcggctgtcg ggcaacacga aatattgtgt
     5401 gctttgcaca gtaccgcagg cggcaagcgt cagggcggcg gcaatcggga agaggcgcat
     5461 tatcggcttc ctttcgggat ggggtctttg ctgctgctgt tgaaaatcag cgcgttgggt
     5521 ttttctttca aagtgttaat gacgggttga acgtctttta aggttttgtc caaactttgc
     5581 aacgtatttt gtacgtcgcc gtagataggc gattgaggcg atacgccttg cagggtttga
     5641 cgtaattctt tcagggtttg gttcagttcg ttcggaatgt tttgcgtctg cggattgccg
     5701 gccagtttgt caatggagct tagggcggca ttggcagatt tgagtgcgga cttgagctcg
     5761 gcaagcgaac cgttcaattc ggcaaccgtc ttatccaaag gcagtttgtc gaacttgtcc
     5821 agcaaatcca ccactttcgc ctgcaaatcg tccaaaccgc cgccccgtgt ggcgatgacg
     5881 gtatcgcctg cataaacggt atgcggtcgc agcttgggcg aagacgaagg ctgatcgttc
     5941 aactcaatca ttttgctgcc ggtcagcagg ttgttgctgg agatggtggc ggtcaggcct
     6001 ttgtttaaag ccgtctgaaa ttgttgtttc caatgctctt tgctttgttc gtcggcattg
     6061 atttccaaac gggaaggctc gatgcggatg cgtacgggaa tccagccgtt ttcaaataaa
     6121 tgcaggctgt cgttgcggtc gaaataaggg acatcggaaa ccacgccgac attcagccct
     6181 ttgtactcga cgggcgaacc gacggtcagt ccgcgcacgg attgtttgaa aaacgcggtg
     6241 tagtacagcg agcggtcgtc aggcaggttg gcgacttcgc tgcggctgtc gtaaagcgtg
     6301 aagctgtctt cgcttttaac gtttttactg tttttggttt tcggcgaatc aaatgaaatc
     6361 gcgcccgaca gcagggcagg cagaggggcg gaattgagtt tgatgccgct gcctgtggtt
     6421 tcgatattga tgccgctttc cagccaaaaa cggctggcgg aatgaatcag tttgtcgttg
     6481 gggctttgga tgaagatggt gtaatgcacg ctttggtcgg acgggtcgaa atgcgcgctt
     6541 tcgacttgcc cgaccataaa gttttcatac aaaacagggc tgttgacgtt gaggatgcgg
     6601 tcgtttttac caatcaaatt caagcgcagc ccgctttgcc cgatggcggt aacgggcgga
     6661 atgtcctgca cttggaacac gtcttttgcc tcgtcgcttt tgccgggtgt aaaagcgatg
     6721 tacgaacccg aaagcagcgt acccaaaccg gttacgccgc tttggtcgat acgcggcttg
     6781 accacccaaa actgtgtgtc gctgcggatg aggccggata cgtccgcatt gagttgggcg
     6841 gttacttcca cgcctttctg gtcgtcgcgc agtttgattc gggtaacgcg tccgacatcg
     6901 atgctcaata ccttaatgac cgtattgttg acctcaatgc cttccgcgct gtccatcaag
     6961 agcgtaacca caggccccct gttgcggatt tccttaatcc acagccagcc accggcaatc
     7021 agcgcaatca gcgggaccag ccagacggcg gagagcaagg tgttgttttt gcggacgcgt
     7081 gcttgggcgt gtccgtttgg aggagggctg ttgtcagtca tgtttttccg tttcattgaa
     7141 agcaatgccg tctgaagcgc gtttgtccca aagcaggcgc gggtcgaaat aataggcgga
     7201 cagcatcgtc agaatcacga ccaggcagaa atagactgcc gcactgcccg gaatgacgcg
     7261 cgcggcataa gtgtggaacg aacacatcaa aataataatc acaaaaatat caatcatcga
     7321 ccagcggccg accgcttcgg tgatgcggta gaggtgcgac aatttctttg cgcccgttgg
     7381 caaagcgaag cgggcggacg caatcaaaac cgacattgcc gcaatcttca gtaccggcac
     7441 caaaatactc gcgctgaaaa taaccgccgc aatcagcctg tcgccctcgt cccacatata
     7501 agcgatgccg ttaaggatgg tattgacctc cgtggcggca ggattggacg aaatcataat
     7561 cggcaggata ttggcaggga aatacaaaat aaccgccgcc gtcagaaacg ccgacgaaat
     7621 actcagactt ttcggccgtc ggcggtacag ttccgcaccg cacacgccgc agggggattc
     7681 ggcaccgtct cggaaataca ggcagcggct gcaacaggtt ttgccttccg atgccgtctg
     7741 aaccgcatta tcccccgtca gccgcccgat ttgaaaatat acccaatgct ggggaacgga
     7801 taccgaagtc cgaatcagca taaccgacag cgcgaacatc agataaaacg ccggcccgaa
     7861 gcgaacctct gccacagacg agagcttgat atacgccacc aaagtggaaa caaaaaacac
     7921 atccaccatc atcgcctgcc tcaagcgcac catcacacgc gttgccaaac gcagcgcagg
     7981 atacgcctgt ttccgtatca gcgcggcata gacatacagg cacagcagca gaaacagaac
     8041 cggcgcgccg aaggtcagca caaacatcac ttcggccaaa aaaccataat cctgaaacac
     8101 catcaggcgc atcatctcgg gcagcgaaag gacggatgcc gcacccggta tcccgacctc
     8161 gatatacgtc ataccgtaag caaacgccat caaaatcagc gaagccgccg cataagcggg
     8221 cggggcggaa aaaggatgcc tgcccaccct gaagagtttg tgtccgcaac ggggacagaa
     8281 cgccgcttct ccactgtcca accggggtac atccgcgcgg cagccgcatt ccggacaatc
     8341 taccgtatgc ggcggcaggg cttcgtcgca gcagttgccg cgaaggctgt gggggatatt
     8401 ttcggcaaac ggcttcatcg ggaaagtggc aaatataagt ggcggcatta taaaggaaaa
     8461 aaggcgatac acccaatcag gatgcaaaac cggcaggttc ggcagtatga acaaaccctt
     8521 ggtgtccggc aaaaatattt tttgagcggt tgtccgccga ctgccgaacc catttgataa
     8581 aatcccgatt ctgtaaagaa tagtaaaata cctgcttaaa aacatcacgc aaatgatatt
     8641 ttttccaaaa atatgtgata ataaatttcg tttatgattc tttattattt ttatagtgga
     8701 ttaacaaaaa tcaggacaag gcggcgaagc cgcagacagt aca
//
LOCUS       DAXHNE010000078         8718 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.078, whole genome shotgun sequence.
ACCESSION   DAXHNE010000078 DAXHNE010000000
VERSION     DAXHNE010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8718
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.078"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(155..1597)
                     /gene="aldA"
                     /locus_tag="ACFM4Z_001333"
     CDS             complement(155..1597)
                     /gene="aldA"
                     /locus_tag="ACFM4Z_001333"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFC6021362.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRGRADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1705..2622)
                     /locus_tag="ACFM4Z_001334"
     CDS             complement(1705..2622)
                     /locus_tag="ACFM4Z_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6021363.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRCRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWDLPPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFADGA
                     HQVNRLTDALNISERTLQRRMRDHFGITTSEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            2795..3595
                     /locus_tag="ACFM4Z_001335"
     CDS             2795..3595
                     /locus_tag="ACFM4Z_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6021364.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSVMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            3643..4419
                     /gene="mlaE"
                     /locus_tag="ACFM4Z_001336"
     CDS             3643..4419
                     /gene="mlaE"
                     /locus_tag="ACFM4Z_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFC6021365.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLSLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            4470..4964
                     /gene="mlaD"
                     /locus_tag="ACFM4Z_001337"
     CDS             4470..4964
                     /gene="mlaD"
                     /locus_tag="ACFM4Z_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFC6021366.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGNSGKTYTVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5001..5591
                     /locus_tag="ACFM4Z_001338"
     CDS             5001..5591
                     /locus_tag="ACFM4Z_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFC6021367.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            5648..5926
                     /locus_tag="ACFM4Z_001339"
     CDS             5648..5926
                     /locus_tag="ACFM4Z_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFC6021368.1"
                     /translation="MHTELNNGTLHISGDITVKTLTGEAFERFQQQCRLKETIAVDFG
                     GVKRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            5923..6750
                     /locus_tag="ACFM4Z_001340"
     CDS             5923..6750
                     /locus_tag="ACFM4Z_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HFC6021369.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            6875..7237
                     /locus_tag="ACFM4Z_001341"
     CDS             6875..7237
                     /locus_tag="ACFM4Z_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HFC6021370.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7237..7620
                     /locus_tag="ACFM4Z_001342"
     CDS             7237..7620
                     /locus_tag="ACFM4Z_001342"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC6021371.1"
                     /translation="MKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELAG
                     LILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITLM
                     PVHAATQRTVSLPATLARALEALSE"
     gene            7617..8105
                     /locus_tag="ACFM4Z_001343"
     CDS             7617..8105
                     /locus_tag="ACFM4Z_001343"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HFC6021372.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8187..8315)
                     /locus_tag="ACFM4Z_001344"
     CDS             complement(8187..8315)
                     /locus_tag="ACFM4Z_001344"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFC6021373.1"
                     /translation="MAGVTVGRGSVVGAGAVVTKDIPPYSLAAGNPAVVKKNLPEG"
     gene            complement(8321..8695)
                     /locus_tag="ACFM4Z_001345"
     CDS             complement(8321..8695)
                     /locus_tag="ACFM4Z_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HFC6021374.1"
                     /translation="MGSKFFFLLLRFAGSVLPPSHMRGIVGRRVRGFLARRVSPHIGR
                     GVNIERGAYVFPDTVLGDGSGIGANCEICRGPVVGKNVMMEPECLFYSNNHKFDRSKN
                     ALRATRKSVRLRWRTMSGRGAG"
BASE COUNT         1947 a         2512 c         2282 g         1977 t
ORIGIN      
        1 tgtactgtct gcggcttcgt ttaaatttaa tccgctatac aaagacaaaa acatcgacct
       61 cggtcatgac tgattgccga tgaagcaata aaaatgccgt ctgaacctga aaacgggttc
      121 agacggcatt ttctatcggg gttttaaagc ggcattaaat gtcggtttcc aaataaacga
      181 cttgggtttg cagatattct tccaaaccgt gtttgccgtc cgcgccgccg ataccggatt
      241 ttttccaacc ggcgtggaaa ccctgcatcg cttcaaagtt ttcgcggttg atgtaggttt
      301 cgccgaattg caggcggcgg gtaacgtaga aggcttcgtt taaattagtc gtataaacag
      361 aactggtcag accaaactcg caatcgttag ccagtgcgat gacttggtcg agcgtgtcga
      421 aagcggaaac gggcagcacg gggccgaagg tttcttcttt cataatgtcc atactgttgt
      481 cggtgtcggt cagcagggtt ggctcgaaga aatagccgcg cccttcggcg cgtttgccgc
      541 cgcagaccag tttcgcacct tgtttgactg cccgttccac tttttcggca acggctttga
      601 cggcgcgctc ttcaatcagc gggcccattt ccagcgcgcc ggcttcggct tcggcagggt
      661 tgccgtagcg cacgcctttc atcgcggcgg tcattttttc gataaacgcg tctttcaggc
      721 tgctgtggac atagacgcgc tcggcgcagt tgcagatttg accagtgttg ccgacgcgcg
      781 aagccaagat ggatttcacc gccaaatcca aatccgcgtc tttcaaaacg atggcggggg
      841 ctttgccgcc gagttccagc gaaactttgg tgatgttggc ggaagcggct tccatcactt
      901 ggcggcctgc ttcgacggag ccggtcaggc tgaccatatc gacttgcgga tgggcagaca
      961 aggcattgcc gatttccgca cccggaccgt tcaccacgtt gaacacgcct gcgggcaatc
     1021 cgaccgcatc gacgatttcg gcgaagatgt ggcagttgat cggggttacg ctgctgggtt
     1081 tgacaacgat ggtgttgccc gttactaagg cgggacccat tttgcgggca atcaggaaga
     1141 aagggaagtt ccacggcaaa atgccggcaa ttacgcccag cggacgtttg aacaataaaa
     1201 tattttcacg cgggcggtcg ctttggatga tttcgccttc gtaacggcgc gcccattcgg
     1261 cttggtaatc gagatagtcg gcggtgaaca tcacttctac gcgcgccaag tctttggttt
     1321 tgccgccttc ggcaacgatg gtgtcggtca gctcgtcggc gcgtccgcgt atgccttggg
     1381 cgattttgcg caaatacgcg ccgcgttcga ccgcaggcag gcgttcccaa gccggttgcg
     1441 ccgcgcgtgc cgccgccaca gctcggtcaa cgtctgcttt tccgcctttg ggttcgcggg
     1501 cgatggtttc ttcggtggaa gggttcaata cgtcgcgcca ttcgccgttg aagtcgtttt
     1561 caaagcgtcc gttgatgtac atggccaatt gtttcatttc gggttctcca gttttgtagt
     1621 cagatgtagt tttagtttat tcccaaataa attaccgtac aagtttcttt acacgcggat
     1681 ttcgggtttg aaatcaaatg cggcttaccg gttttctttt ttggtttccg caggcgtgct
     1741 gccgaaatat tgcctgaatg cctgagtaaa gctggaaacg tggcggtagc cgcataaata
     1801 tgcggtttcg cctatgcttt tccccccgtt ttgcaacagg tagagcgcgt gctgcatttg
     1861 tttgtggtgc agccactcgc ttgtggtaat gccgaaatgg tcgcgcatac ggcgttgcag
     1921 cgtcctttcg ctgatgttca gcgcgtctgt cagccggttg acttggtgtg cgccgtcggc
     1981 aaacgcggca tttagggtgc ggctgaagtc ttcagacggc atagcgtctg cttccgccgt
     2041 ttgccccgcc gccggcccga tgccgtctga aaccgtgtcc cacaagtccg acagcagccg
     2101 caacacgtcc gcctcgcggc gcaatgtttc gcccaaatgc ccctttggga cggctttcag
     2161 gcaggatgcc gccaagccgc gcaggttcgg gggcaaatcc catatcctga ccggttcgcg
     2221 gtaaagcagg ggtgcgaaac gcgcgtattc cggacgcagc agccattgtt ccataccttt
     2281 aatggtcatt ttgaccgttt tgccgcctcg gtaaagatag cggctgaaca ggacttcttc
     2341 cccgacagca attaggacaa tcttgccgcc gtccgcatcg atttggaagc ggcagcggtt
     2401 gatgccgaag tccaaactgc cttccagcaa gagcacgaac gacacataag gttcggcgag
     2461 gcggctgctg caaaaatcac accgtgccgt tactgtgccg ccgtggatgg aaatgccgtt
     2521 ggacaaagta tcgaaacggt agctgccctc cacatagctg ccgccgccgc ttttgaccag
     2581 attgacgaac cggctgctgt tcaaatgttc tgctttttcc atagttttct gataaaggtt
     2641 atcatttgaa agataacatt tttcgccaca gcaaacaatc cgccgccgac aacggcattt
     2701 tcacaacatt taccgtccgt atgccgtctg aaaattgccc gtcttcccga taagccttaa
     2761 aatacacgcc gtcaaaccta ccgtccgtcc gtacatgagc ccatccccct ttatcgaaat
     2821 gaaagacgtc gcttttgcgt atggcgaccg cccgattctg aacgacatca atttcagcat
     2881 tccgcaaggc aattttgccg ccgtgatggg cggttcgggc agcggcaaaa ccacgctgat
     2941 gcggctgatt acgggtcaga tccgtccgca gtccgggcag gttttgattg aaggacggga
     3001 tttggcgggc ttttcggctg acgaactcta cgaacaccgc cgccgcatgg gcgtattatt
     3061 ccaacacggc gcgctgttta ccgatttgtc ggtattcgac aatatcgctt ttccgatgcg
     3121 cgaactgaca cgcctgccgg aagcggtgat tcgagatttg gttttgttga aactgaacgc
     3181 ggtcggtttg cgcggcgtgg aaaacctgat gccgtccgag ttgtccggcg ggatgtcgcg
     3241 ccgcgtcgcg cttgcccgca cgattgcgct cgaccctgaa attatgttgt acgacgaacc
     3301 gtttaccggc ctcgatccga tttccttggg cgtgattgcc cacttgatca gccgcgtcaa
     3361 taaggctttg cgttcgacca gcgttatggt aacgcacgac attgaaaaat ctttggaaat
     3421 cgtcgatcag gtgatttttt tggcgcacgg cgaaattatg ttctccggct cgccgcagga
     3481 aatgcgcgaa ctggattcgc cttgggtgcg ccagtttgtc ggcgggctgg cagacggccc
     3541 cgtagcatac cgctatccgg cgcaaacgtc gttgcagcag gatttgctcg ggtaaatgag
     3601 caggtgccgt ctgaaaccgt tgaactttac atatagaaat ttatgaattt tatccgttcc
     3661 gtcggggcga aaaccctcgg ccttattcaa tccctcggca gtattacgct gtttctgctg
     3721 aacattctgg cgaagtccgg tacggctttc gtccgtccgc gcctgagcgt gcgccaagtg
     3781 tattttgccg gcgtgctgtc ggtgctgatt gttgccgttt cggggctgtt tgtcggtatg
     3841 gtcttgggtt tgcagggcta tacgcagttg tcgaaattca aatccgccga tattttgggc
     3901 tatatggttg cggcttcgct gttgcgcgaa ctgggtccgg tgttggcggc gattctgttt
     3961 gccagcagcg ctggcggtgc gatgaccagc gaaatcggtt tgatgaaaac gaccgaacag
     4021 ctcgaagcga tgaacgtgat ggcggtaaac cctgtcgccc gcgtggttgc gccgcgcttt
     4081 tgggcgggcg tgttttccat gccgcttttg gcttcgattt tcaacgtggc gggtattttc
     4141 ggcgcgtatt tggtcggtgt aagctggctg agcttggaca gcggcatttt ctggtcgcaa
     4201 atgcagaaca acatcacgat acattacgat gtaatcaacg gtctgatcaa atccgccgcg
     4261 ttcggcgtgg cggtaacgct gattgccgtg catcagggct tccactgcgt cccgacctcg
     4321 gaaggcattt tgcgcgccag cacgcgcacg gtggtttcgt ccgccctgac gattttggcg
     4381 gtcgatttta tattgaccgc gtggatgttt acagattgac agggcgtctg aagacgaaac
     4441 gcggaacatc ggatattcaa ggaactttaa tgaaaaagaa catattggaa ttttgggtcg
     4501 gactgttcgt cctgattggc gcggcggcgg ttgcctttct cgctttccgc gtggcgggcg
     4561 gcgcggcgtt cggcaattcc ggcaagactt ataccgtata tgccgatttc ggcgacatcg
     4621 gcggtttgaa ggtcaatgcc cccgtcaaat ccgcaggcgt attggtcggg cgcgtcggcg
     4681 ctatcggact tgacccgaaa tcctatcagg cgagggtgcg cctcgatttg gacggcaagt
     4741 atcagttcag cagcgacgtt tccgcgcaaa tcctgacttc gggacttttg ggcgagcagt
     4801 acatcgggct gcagcagggc ggcgacacgg aaaaccttgc tgccggcgac accatctccg
     4861 taaccagttc tgcaatggtt ctggaaaacc ttatcggcaa attcatgacg agttttgccg
     4921 aaaaaaatgc cgacggcggc aatgcggaaa aagccgccga ataaacgtat gccatcatat
     4981 tcaaactagg acacttaatc atgaaaaaat cctccctcat cagcgcattg ggcatcggta
     5041 ttttgagcat cggcatggca tttgccgccc ctgccgacgc ggtaaaccaa atccgtcaaa
     5101 acgccactca agtattgagc atcttaaaaa gcggtgatgc caacaccgcc cgccaaaaag
     5161 ccgaagccta tgcgattccc tatttcgatt tccaacgtat gaccgcattg gcggtcggca
     5221 acccttggcg caccgcgtcc gacgcgcaaa aacaagcgtt ggccaaagaa tttcaaaccc
     5281 tgctgatccg cacctattcc ggcacgatgc tgaaattaaa aaacgccaac gtcaacgtca
     5341 aagacaatcc catcgtcaat aaaggcggca aagaaatcat cgtccgcgcc gaagtcggcg
     5401 tacctgggca aaaacccgtc aacatggact tcaccaccta ccaaagcggc ggtaaatacc
     5461 gtacctacaa cgtcgccatc gaaggcgcga gcctggttac cgtgtaccgc aaccaattcg
     5521 gcgaaattat caaagcgaaa ggcgtggacg gactgattgc cgagttgaag gctaaaaacg
     5581 gcagcaagta atccataagc catgccgcca gactgaagat ggcatggatt ttttcctacc
     5641 gaacactatg catacagaac tcaacaacgg aacactgcac atcagcggcg acatcaccgt
     5701 caaaaccctg accggggagg cttttgagcg tttccagcag caatgccgtc tgaaagaaac
     5761 cattgccgtc gatttcggcg gtgtcaaacg tgccgattcc gcctgcgtgt cgctgctgct
     5821 cgaagtgctg cgcggctgca aaggcagcgt caggctgacc ggcattcccg aatccgtgcg
     5881 cgcgctgtcc gaactgtacg aaatcaaaga ctggctgaaa tcatgaaaaa aaccgcctat
     5941 gccttcctcc tgctgatcgg gttcgcttcc gcccctgcat ttgccgaaac ccgccccgcc
     6001 gacccttatg aaggctacaa ccgcgccgtt ttcaaattca acgaccaagc cgaccgctac
     6061 attttcgccc ctgccgcgcg cggctaccgc aaagttgcgc cgaaacccgt ccgcgccggc
     6121 gtgtccaatt tttttaataa cctgtgcgac gtggtcagct tcggcagcaa tatcttgcgc
     6181 ttggacatca aacgcgcaag cgaagacctt gtccgcgtcg gcatcaacac cactttcggt
     6241 ttgggcgggc ttatcgacat cgccggcgcg ggcggcattc ccgacaataa aaacaccttg
     6301 ggcgacacgt ttgcttcgtg gggatggaaa aacagcaatt atttcgtgtt gcccgtctta
     6361 gggccgtcca ccgtccgcga cgcgctcggc acgggtatta cctccgttta ttcgcccaag
     6421 aatatcgtct tccgcacccc tgtcggacgc tggggcacga ctgccgtatc cgccgtcagt
     6481 acgcgcgaag gcctgctcga tttgaccgac agcctggacg aagccgccat cgacaaatac
     6541 agctacacgc gcgacctcta tatgaaagtc cgtgcgcggc agaccggtgc aacacctgcc
     6601 gaaggtacgg aagataacat cgacatcgac gaattggtcg aaagtgccga aaccggcgcg
     6661 gcggaaactg ccgttcaaga agattccgta tccgaaacac aggcagaagc agcaggggaa
     6721 gccgaaacgc aacctggaac acaaccttaa agccatgccg tctgaaaatc cgtgttcgga
     6781 cggcattccc ttctgtcgga acggacagtc cgaaaccaca gattgggcta taatctcttt
     6841 tttgcgcata ccgcgcgaca gcaaaggaaa aaccatgtac gaagtcaacc gcagcgtatt
     6901 cgtcctcatc cctttagaac ctttttggaa ctggctgcaa accctgccgg gcaaccacct
     6961 cgacggactg actcttgaag acatccaagc cgacgccaat tcctaccttg tccgcccgtg
     7021 cgaaaccgcc gacgaagtat gggacgaaat cgaagcccgt tttgaagaca ttttcgccgc
     7081 cgaacttgcc gactggtgcg aagacgaacg cgagtggcct gcgctcgatg ccgacatttt
     7141 caacgaatgg ttcgacatcc agctttctac cgtcatcacc gacctcgaac acgaaccgct
     7201 tgcccgcgaa gccttccaac ccatcaacct gaactgatga agctgaccgt ccgcaactac
     7261 catctcgacg gctacggcca tgtcaacaat gcgcgctacc tcgaattttt cgaagaagcg
     7321 cgctgggcgt tttttgaaga acgcggactg ctgcacgagt tggcaggcct catactgatt
     7381 gtcgcccgca tcgacatccg atacagccgc cccgccgtcg aaggcgacgt attgcagttt
     7441 tcatgccgcc tgaaaacccc cggcacgcgc cgtattgtgc tgacccagac gattacactg
     7501 ccaaacggca aaaccgccgc agaagccgac atcaccctga tgcccgtcca cgccgccaca
     7561 caacgcaccg tcagcctgcc cgccaccctt gcccgcgcat tggaagcctt gtccgaatga
     7621 aaaaaatact caccgccgcc gtcgtcgcac tgatcggcat cctccttgcc atcgtcctca
     7681 tccccgacag caaaaccgcg cccgccttct ccctgcccga cctgcacgga aaaaccgttt
     7741 ccaacgccga cctgcaaggc aaagtaaccc tgattaattt ttggtttccc tcctgtccgg
     7801 gttgtgtgag cgaaatgccc aaaatcatta aaacggcaaa tgactataaa aacaaaaact
     7861 tccaagtcct tgccgtcgcc cagcccatcg atccgataga aagcgtccgc caatatgtca
     7921 aagactacgg tttgccgttt accgtcatgt atgatgcgga caaagctgtc ggacaggcgt
     7981 tcggcacaca ggtttatccg acttccgtcc ttatcggcaa aaaaggcgaa atcctcaaaa
     8041 cctacgtcgg cgaacccgat ttcggcaaac tctaccaaga aatcgatacc gcgctggcac
     8101 aatagcgatg ccgtctgaaa cgcaaaaaaa cagaaacgat gttcctgttt tttcagatca
     8161 tccgccccga cacgttcaga cggcattcaa ccttccggca gattcttttt caccactgcc
     8221 ggattgcctg ccgccaaaga gtagggcgga atgtcttttg taaccaccgc gcctgcgccc
     8281 acgaccgaac cgcgtccgac ggttacgccc gccattacaa tcaccctgcg ccccggccag
     8341 acatcgtcct ccaacgtaat cggacggatt tccgtgtagc cctcaaagcg ttttttgaac
     8401 ggtcaaactt gtggttattt gaataaaaca gacattccgg ctccatcatc acatttttgc
     8461 cgaccaccgg cccacggcag atttcacagt ttgccccgat gcccgagccg tcgcccaaaa
     8521 ccgtatccgg aaacacatac gccccgcgtt cgatattgac cccgcgtccg atatgcggag
     8581 aaacccgccg cgccaaaaaa ccgcgtaccc gtctgccgac gatgccgcgc atatgcgacg
     8641 gcggcaacac cgaaccggca aaacgcagca gcaggaaaaa gaattttgat cccatcaagc
     8701 ctccctcaaa agaacttt
//
LOCUS       DAXHNE010000079         8700 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.079, whole genome shotgun sequence.
ACCESSION   DAXHNE010000079 DAXHNE010000000
VERSION     DAXHNE010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8700
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.079"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            191..1726
                     /locus_tag="ACFM4Z_001346"
     CDS             191..1726
                     /locus_tag="ACFM4Z_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC6021375.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLNIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     DEK"
     gene            1723..1815
                     /locus_tag="ACFM4Z_001347"
     CDS             1723..1815
                     /locus_tag="ACFM4Z_001347"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HFC6021376.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1875..3095)
                     /gene="lysA"
                     /locus_tag="ACFM4Z_001348"
     CDS             complement(1875..3095)
                     /gene="lysA"
                     /locus_tag="ACFM4Z_001348"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFC6021377.1"
                     /translation="MTLFCEQVPYPRLAEEFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELVRVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAERLGKTASVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDTLVA
                     EGIVLEHLDLGGGVGIVYQDESVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKNIATLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVDGGGYRLIRRR
                     ETLEQQMANELACL"
     gene            complement(3106..3276)
                     /locus_tag="ACFM4Z_001349"
     CDS             complement(3106..3276)
                     /locus_tag="ACFM4Z_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC6021378.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QSKPQSAPQTQK"
     gene            3347..3670
                     /gene="cyaY"
                     /locus_tag="ACFM4Z_001350"
     CDS             3347..3670
                     /gene="cyaY"
                     /locus_tag="ACFM4Z_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFC6021379.1"
                     /translation="MMTESEFIRMSEELFEHIEDQIDENGWDFDCQFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIEEL"
     gene            3697..4713
                     /locus_tag="ACFM4Z_001351"
     CDS             3697..4713
                     /locus_tag="ACFM4Z_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFC6021380.1"
                     /translation="MNTRPFYFGLIFIAIIAILANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGALLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTALLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAEPVTKAESHKVSVAIAVV
                     VIFGTLAIFTYPLFYTWSQHLINAHQFGIYVGSSVHEVAQVYAIGENIDPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSNGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTQASAIKKAGLKPFVLGILTYLWLVVGGFLVNYGI
                     SKLI"
     gene            4739..5158
                     /locus_tag="ACFM4Z_001352"
     CDS             4739..5158
                     /locus_tag="ACFM4Z_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFC6021381.1"
                     /translation="MAQLPLYRTAEIKDFTVGTPEVLQSFFEHVPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTLAALCINDYIHAVYDFVAQ
                     AGYCRNGFPEAQGEWLKVADRVLDDELLDKILSRKPT"
     gene            5180..5686
                     /gene="luxS"
                     /locus_tag="ACFM4Z_001353"
     CDS             5180..5686
                     /gene="luxS"
                     /locus_tag="ACFM4Z_001353"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFC6021382.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCVPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            5832..8627
                     /gene="polA"
                     /locus_tag="ACFM4Z_001354"
     CDS             5832..8627
                     /gene="polA"
                     /locus_tag="ACFM4Z_001354"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFC6021383.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSSETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVIEHAS
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHAELSDGIESLRRTAPKWAQLVVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEMPFEKQAEKATAPEKLDYQA
                     VTTEAQFAALLDKLSRADTIGIDTETTSLDAMNASLVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQISFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDAKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPDMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPPGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSPEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGFVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVSRWLVSDDLKSKLIMQVHDELVLEVVETELDFVKEKLPQIMAKVGGGLLD
                     VPLVAEVGVGENWEEAH"
BASE COUNT         2039 a         2260 c         2284 g         2117 t
ORIGIN      
        1 aaatttttcg ttttcaaacc ttcaccgctt gccatcagcg ttaaattttt ttacgataag
       61 cacatagatt gtaaacaatc ggccacaagc cggtttgttt tttcagaaga cattatccct
      121 gtcagacgct gtttctatat atttcgccta taatggcttg tttttaataa ataattcaag
      181 aggtatcaac gtgtctgatt ccaagacgaa agaacgcgcc acattcggca cgcgccgcgc
      241 gtttatgatt gccgccatcg ggtccgccgt cggcttgggc aatatttggc gtttccccta
      301 tattgctttt gaaaacggcg gcggcgcgtt catcctgccc tatctggtcg cgcttctgac
      361 ggcgggcatc ccgctgctgc tgctcgatta tgccatcggc caccgttacc gtggttctgc
      421 gcccttggct ttccgccgcc tcggacgatg gtttgagccg gtcggctggt ggaacgtgat
      481 gaccaatatc gtcatctgca tctattacgc ggtaattatc ggttgggcgg caagctatac
      541 ctattattcg gtcaacgctg cctggggtgc ggatccgcag ggttttttct ttaaggactt
      601 cctgcaaatg gcgggcccgg aagccttggg tttggatttt gtcggcaaag tcgccggtcc
      661 tttggcgggc gtgtgggttt ttaccgccgc cattatggcg ttgggcgtgc aaaagggcgt
      721 ggcgcgcgcc tcgtcgttct ttatgccgct gcttttggtg atgtttttga ttatggtcgg
      781 catttcacta accctgccgg gtgcggcaaa gggcttggac gcattgttta cgcccgactg
      841 gtcgaaactc gccgattcca aggtctgggt ggcggcatac gggcagattt tcttttcgct
      901 ttccatctgc ttcggcatta tggttaccta ttcttcttat ttgaagaaaa aaaccgactt
      961 gggcggaacg gggctggtgg tcggttttgc caacagcagc tttgaactgc tcgcgggcat
     1021 cggcgtgttt gccgcattgg gctttatggc gcaggcgggc ggtaaggcgg tcaacgaggt
     1081 tgcctcaggc ggcatcggtt tggcgtttat cgcctttccg accattatca accaggcacc
     1141 gatgggctgg ctgatcggca tattgttttt cggttcgctg gtattcgccg gcgttacgtc
     1201 gatgatttcc atccttgaag tgattgtggc ggcgattcag gacaagctga acatcgggcg
     1261 cgtcaacgcc acgctgctgg tctgcattcc gatgggcatt gtttccacgc tgctgttcgg
     1321 tacggcgacg gggctgccgg ttttggacgt gatggacaaa ttcgtcaaca cctacggcat
     1381 tgttgccgcc ggctttgttt atgttgccgc catcatcatc agcggcaggc tgccggaatt
     1441 acgcaagcac ctgaacgcct tgtcctccat ccgcatcggc ggcttgtgga cggtctgcgt
     1501 cgtggttacc gtcgtgatgc tcggctatat gctgtttaaa gataccagcg gcctgatgga
     1561 gaaaaattac gaaggttatc cggatggttt cctcaatatt ttcggttggg ggatgtcggc
     1621 ggcgttggtc gtgttcgggc tgctgctgtc gttgctgcct tggaaacacg gtcaggattt
     1681 caacgtcaaa gacgaacacg aacatgaaca aggagacgaa aaatgagtac ttccgccatt
     1741 gtgatgatga ttgtctcaat cgtgataatc tggggagggc tgctgctttc cctgttaagg
     1801 ctgccgaacg agtaagcctt tagagcgtta aaaatgccgt ctgaactgtt tcagacggca
     1861 ttttgatgtt cggcttacag gcaggcgagt tcgtttgcca tttgctgttc caaggtttct
     1921 cgccggcgga tgagtcggta tccgccgccg tcaaccaata cctccgccgc acggttgcgc
     1981 gcgttgtaat tgctcgccat gctggcaccg tatgcgcccg cgctgcggat aagcagcaaa
     2041 tcgccttctt cgcaggcgat ggtgcggtct ttgccgagga agtcgccggt ttcacaaatc
     2101 gggccgacga tgttggcggt cagcgtcgcg atgtttttgg tttcgaccgc ttcgatgtgg
     2161 tggtaggcat cataaagtgc cggacgcatt aaatcgttca tcgccgcatc gaccatcaca
     2221 aagtttttct cttcgccgtg tttgacgaat tcaacgcgcg tcaataacgc acctgcgtta
     2281 ccgaccaagc tacgacctgg ttcgaggatg agtttcagac ggcgtgtgcc gatcagtttt
     2341 tgaaccgctt gggcatatgc gcccaaatcg gggacgcttt cgtcttgata aacgatgccg
     2401 acaccgccgc ccaagtccaa atgttccaaa acaatgcctt cggcgacaag agtgtcaacc
     2461 aaaatcaaaa tgcgttcgca ggcttcgacc agcgggctta agtcggtcag ttgcgaaccg
     2521 atgtggcagt cgatgccgat gattttcaaa ttgggctgtt gtgcggcatg gcggtaggct
     2581 tcgagcgcgt cggcgtaggc gatgccgaat ttgttggctt tcagtcctgt ggagatgtag
     2641 ggatgggttt tcgcatcgac atcggggttg acgcgcaggg agacggacgc ggttttgccc
     2701 aagcgttcgg caaccttttg aatgcggtcg atttcgggaa tgctttccat attgaagcat
     2761 ttcacgcccg cattcagcgc aaactcgatt tccgcctcgc ttttgcctac gcctgaaaag
     2821 atggttttcg ccgcgtcgcc gcctgccgcc aaaacgcgta ccaattctcc gccggacaca
     2881 atgtcgaaac cgctgcccag agaagcaaag tgtttgatga tgctcaggtt gccattggct
     2941 ttaaccgcat agcagacgag cggggagagc tcggcaaagg cagtttggta gtgttcaaat
     3001 gcttcggtca gcgcggattg gctgtacaca taaagcggcg tgccgaattc ttcggcaagg
     3061 cgggggtagg ggacttgttc gcaaaatagg gtcatgtttt cgttttcatt tttgggtttg
     3121 tggagcggat tgtggtttgc tttgaagttg caaaccggtt tggattacgc cgaaacgcgc
     3181 cttgtcgcct tctttgggca ggtagaggtc gcctttgtaa ccgcaggccg agagcaggag
     3241 ggcggttgcc gccgcaaaaa atacgccgta tttcatcggt aaacttcctt cataagcgcg
     3301 aatgtggcaa gattcggtat cttaaacaaa aaacacgcaa aaagccatga tgaccgaaag
     3361 cgaattcatc cgcatgagcg aagaattgtt cgaacacatc gaagaccaaa tcgacgaaaa
     3421 tggctgggat ttcgactgcc agtttgccgg aaacgtcttg accatcgaag ccggagacgg
     3481 cacgcaaatc atcgtcaacc gccacacgcc caatcaggaa ttgtggattg ccgccaaaag
     3541 cggcggctac catttcgccg agcaaaacgg caaatggctg gcaacacgcg acagccgcga
     3601 tttctacgac gttttaaacg aagcactgag cgcggcttcg ggcgaagcgg tagagattga
     3661 ggaattgtga tttgggtgtt atcacggaaa gaaaaaatga acacacgtcc cttttatttc
     3721 ggacttatat ttatcgcgat tatcgctata cttgctaact atttaggaaa cactgatttt
     3781 tcccatcatt atcatatcag tgctttaatt attgctatct tgctgggaat ggcaatcggc
     3841 aataccattt atccgcaatt ttcaacacaa gtggaaaaag gcgttttgtt tgccaaaggc
     3901 gcgcttcttc gcactggcat tgtgctgtat ggtttccgcc tcacttttgg cgatattgcc
     3961 gatgtaggat taaatgctgt cgttaccgat gcaatcatgc taatttcaac cttctttttt
     4021 accgcacttt taggcattcg ttatctaaaa atggataaac aattggttta tctcactggg
     4081 gcaggttgca gcatttgcgg tgcggcagca gtgatggcgg cagagcctgt taccaaagca
     4141 gaatcccata aagtttcagt ggcgattgcc gtagtggtca ttttcgggac gcttgctatt
     4201 tttacttacc ccttgttcta cacgtggtca caacatttaa ttaacgccca tcaattcggt
     4261 atttatgttg gttctagtgt acacgaagtg gctcaagttt atgcgattgg ggaaaatatt
     4321 gatcctatcg tggcgaatac tgccgtcatt tccaaaatga tccgagtgat gatgctcgcc
     4381 ccctttttat taatgctttc ttggttatta acacgtagta atggagtatc agaaaataca
     4441 tcacacaaaa ttacaattcc ttggtttgct gtacttttta ttggtgttgc catttttaat
     4501 tcttttgatt tattaccaaa agaactcgtg aaattattcg ttgaaatcga ttctttctta
     4561 ttaatttcat caatggctgc gcttggctta acgacgcaag caagcgcaat caaaaaggca
     4621 ggattaaaac cgtttgtttt aggaatacta acttatttat ggctagtggt tggtggattt
     4681 ttagtgaact atggaatatc aaaattaata taaaattcac tcaagagagc attacccaat
     4741 ggcacaatta ccgctatatc ggactgccga aatcaaagac tttactgtcg gcacgcccga
     4801 agttttacaa tcatttttcg aacatgtccc ttatggcgtc gtctttgaag acgacggcga
     4861 cacaggttac ttttatgccg cttcgcaaga cgggatttta gatgccttgc acatctacaa
     4921 tgtcgaagat gtatccgaca aacatatccc caatcatgtc ttgatcttat gggacgatgc
     4981 ctgcactttg gcagctttgt gcatcaacga ctacatccat gccgtctatg attttgtcgc
     5041 gcaggcagga tattgccgca acggcttccc tgaagcacaa ggcgaatggc tgaaagtcgc
     5101 agaccgcgtc ttggacgatg aattgctgga caaaatccta tcccgaaaac ctacataaac
     5161 cactcaaaag gataacaaaa tgcccctatt agacagtttc aaagtcgacc acacccgtat
     5221 gcacgcccct gccgtgcgtg tggcgaaaac catgaccacg cccaaaggcg acaccattac
     5281 cgtgtttgac ctgcgctttt gcgttcccaa caaagaaatc ctgcctgaaa aaggcataca
     5341 cacgctggag catttgttcg caggttttat gcgcgaccac ttgaacggca acggcgtgga
     5401 aatcatcgac atttccccga tgggctgccg caccggtttt tatatgagcc ttatcggcac
     5461 gccttccgaa cagcaggtcg ccgatgcgtg gctggcttcg atgcaggatg ttttgaatgt
     5521 caaagaccaa agcaaaatcc ccgagttgaa cgaataccaa tgcggcactt atcaaatgca
     5581 ctcgctcgcc gaagcgcagc aaatcgcgca aaacgtgttg gcgcgcaaag tggcggtgaa
     5641 caaaaatgaa gagctgacgc tggatgaagg gctgctgaac gcctaatccg ccaaaaatgc
     5701 cgtctgaaca agggtttcag acggcatttg ccttttccgt tataatccgg ggttgtgcgg
     5761 gggcgggttt taagccggca tcgtccttcc ctattttttt ctgtccctta tcggttttaa
     5821 gcgggttttt tatgtccaac agacctacac tcctcctcgt tgacggctct tcctatctct
     5881 accgtgcgta tcacgcgatg gggcaaaacc tgaccgcacc cgacggcgcg ccgacgggcg
     5941 cgctgtatgg cgtattgaat atgttgcgcc gtttgcggtc ggaatatccg cacgattatt
     6001 gcgcggtggt ttttgatgcg aaaggcaaaa atttccgcca tcaaatgttt gaagaataca
     6061 aggcgacgcg cccgccgatg cccgacgatt tgcgcccgca ggcggaagca ctgccggatt
     6121 tagtgcgcct gacgggctgg ccggtattgg tgattgggca agtggaggcg gacgatgtga
     6181 tcggcacgct ggcgaaacag ggggcggaac atggtttgcg agtcattgtt tcgaccggcg
     6241 ataaggacat ggcgcagttg gtggatgagc gcgttacgct ggtgaacacg atgagcagcg
     6301 aaacgctgga cattgaaggc gtgaaggcaa aattcggcgt gcgccccgac caaatccgcg
     6361 attatctcgc gctgatgggc gacaaggtgg acaacgtgcc gggcgtggaa aaatgcggcc
     6421 cgaaaacggc ggtgaagtgg ctggaagcct acggctcgtt gcaaggcgtg atagaacacg
     6481 cttcggaaat caagggcaaa gtgggcgaaa acctgcaagc cgcgctgccc caactgccgc
     6541 tgtcgtatga cttagtaacg attaaaaccg atgtggactt gcacgccgag ctttcagacg
     6601 gcatcgaaag cctgcgccgt accgcgccga aatgggcgca actggttgtc gatttcaaac
     6661 gctggggctt ccgcacctgg ctgaaagaag cggaatcaaa catgaatacc ggctcgaccg
     6721 atgatttgtt cggcagcgac agcatcggcg agcaggcggc tttgaatgcg gaaatgccgt
     6781 ttgaaaaaca agccgaaaaa gccaccgccc ccgaaaaact ggattatcaa gccgttacca
     6841 ccgaagcgca gtttgccgct ttgttggaca aactgtcgcg ggcggacaca atcggcatcg
     6901 atacggaaac cacgtcatta gacgcgatga acgcctcgct ggtcggcatc agcatcgctt
     6961 tccaagcagg cgaagcggtt tacatccccg taggacacag cctgaccgcc gcgcccgaac
     7021 agcttgattt gcaagacgta ttaggccgtc tgaaaccgca tttggaaaac cccgccctga
     7081 agaaaatcgg gcaaaacctc aaatacgacc aacacgtttt cgctaactac ggcatcgccc
     7141 tgaacggcat tgccggcgac gctatgctcg cttcctacat catcgaaagc catcttggac
     7201 acggcttgga cgaattgtcc gaacgttggc tgggcttgga aaccattacc tacgaatcat
     7261 tgtgcggtaa aggcgcgaag caaatcagtt ttgccgatgt cgccatcggg caggcgaccg
     7321 aatacgccgc ccaagacgcc gatttcgcct tgcgcctcga agcgcacctg cgcgcgcaaa
     7381 tggacgccaa acagcttgaa atgtatgaaa aaatggagct gcccgtcgcg caagtattgt
     7441 ttgaaatgga acgcaacggt gtgcaaatcg accgcgccga actcgcccgc caaagcgcgg
     7501 aactcggcgc cgagctgatg aagctcgaac aagaagccta cgccgccgca gggcagccgt
     7561 tcaacctcaa ctcgcccaaa cagctgcaag aaatcctgtt cgacaaaatg ggcatcccga
     7621 caaaagggct gaaaaaaacc gccaagggcg gcatttccac caacgaggcc gtgctcgaac
     7681 agctcgcgcc cgactacccc ttgcccaaaa tcatcctgca aaaccgcagc ctggcgaaac
     7741 tcaaatccac ctacaccgac aaactgcccg acatgatttc tccccgggat aaccgcgtgc
     7801 ataccaccta cgcccaagcc gtcgccatta caggacgctt ggcaagcaac aaccccaacc
     7861 tgcaaaacat ccccatccgc accgccgaag gccgccgcgt gcgccgcgcc tttaccgcgc
     7921 caccgggcag cgtcatcgtt tccgccgact attcccaaat cgaactgcgc atcatggcgc
     7981 acctctctgg agacaaaacc ctcattgccg cattccaaaa cggcgaagac gtacaccgcc
     8041 gcaccgccgc cgaagtgttc ggcactgcgc ccgaaaacgt ctcgcctgag caacgccgct
     8101 acgccaaaac catcaacttc ggcttaatct acggtatggg gcaatacggc cttgccaaat
     8161 cattgggcat cgacaacctt tccgccaaaa actttatcga ccgctacttc gcccgctacc
     8221 ccggcgtcgc cgaatacatg cagcgcacca aagaacaagc cgccgcccaa ggcttcgtcg
     8281 aaaccctgtt tggccgccgc ctctacctgc ccgacatccg caacaaaaac gccaacgccc
     8341 gcgccggagc cgaacgcgcc gccatcaacg cccccatgca aggcaccgcc tccgacctta
     8401 tcaaacgcgc catgatagac gtgtcccgct ggctggtttc agacgacctg aagagcaaac
     8461 tgattatgca ggtgcatgac gaactggtgc tggaagtcgt tgaaaccgaa ctggattttg
     8521 tcaaagaaaa actgccgcag attatggcga aagtgggcgg cggattattg gatgtaccgc
     8581 tggtggctga ggttggcgta ggggagaatt gggaagaggc acattgattg aaaggtgtta
     8641 ttatgctatc tttatttaaa taaaatttaa tttttggtat attttttcta aatgttccta
//
LOCUS       DAXHNE010000080         8669 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.080, whole genome shotgun sequence.
ACCESSION   DAXHNE010000080 DAXHNE010000000
VERSION     DAXHNE010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8669
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.080"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            319..2433
                     /locus_tag="ACFM4Z_001355"
                     /pseudo
     CDS             319..2433
                     /locus_tag="ACFM4Z_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            2675..4141
                     /locus_tag="ACFM4Z_001356"
     CDS             2675..4141
                     /locus_tag="ACFM4Z_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6021384.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFENRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(4342..5310)
                     /locus_tag="ACFM4Z_001357"
     CDS             complement(4342..5310)
                     /locus_tag="ACFM4Z_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021385.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSETVALATPVWQKMGMHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWGGTNGGTQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRIGKIKRLDIATDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            5387..6679
                     /locus_tag="ACFM4Z_001358"
     CDS             5387..6679
                     /locus_tag="ACFM4Z_001358"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFC6021386.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIALTERTERVRPQRV
                     EQNLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAMRLMYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAEGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            complement(7182..7868)
                     /locus_tag="ACFM4Z_001359"
     CDS             complement(7182..7868)
                     /locus_tag="ACFM4Z_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFC6021387.1"
                     /translation="MYAFTDAQQQKALFRLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
     gene            complement(7945..8418)
                     /gene="queF"
                     /locus_tag="ACFM4Z_001360"
     CDS             complement(7945..8418)
                     /gene="queF"
                     /locus_tag="ACFM4Z_001360"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HFC6021388.1"
                     /translation="MSRNNEELQGISLLGNQKTQYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ"
     regulatory      complement(8423..8467)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         2080 a         2120 c         2555 g         1914 t
ORIGIN      
        1 tcccgttatt tccacaaaac agaaaatcaa aaacagtaac ctgaaatccc gtcattcccg
       61 cgcaggcggg aatccggctt gttcggtttc ggttcttttt cttgtttcgg gtgatttcta
      121 aaccgtcatt cccgcgcagg cgggaatcca gacctttaaa ccccgaccat ccttgataaa
      181 ttcttgcggc attaaaattc tagattcccg ctttcgcggg aatgacggcg gagggttttt
      241 tgcttttcct gatttttcat tgcgatgtag tataatgtag tatataatca ttataatttt
      301 aacacttgac aaaggaaaat ttctcatgac actgaaagca agcaagcaag caagcaagca
      361 agcaagcaag caagcaagca agcaagcggt cgggttaatc tattaacatt atctgtttta
      421 tcgctgtttt gcacgccata tgtttgaggt tcggatgcgt acgatcccgt caaagaagcc
      481 gagattaaaa acaaatttat tttagaagcg gcggaagaca gaaattccca cgtttggcgc
      541 ggcccgtgca gcatatcttt tgattgcttc ggtatgttca gagctcagct tggttcaaat
      601 actcgttcta ccaaaatcgg cgacgatgcc gatttttcat tttcagacaa gccgaaaccc
      661 ggcacttccc attatttttc cagcggtaaa accgatcaaa attcatccga atatgggtat
      721 gacgaaatca atatccaagg taaaaattac aatagcggca tcctcgccgt cgataatatg
      781 cccgttgtca aaaaatatat tacagagaag tatggggctg atttaaagca ggcggttaaa
      841 agtcaattac aggatttata caaaacaaga ccggaagctt gggcagaaaa taaaaaacgg
      901 actgaggagg cgtatatagc acagtttgga acaaaattta gtacgctcaa acagacgatg
      961 cccgatttaa ttaataaatt ggtagaagat tccgtactca ctcctcatag taatacatca
     1021 cagactagtc tcaacaacat cttcaataaa aaattacacg tcaaaatcga aaacaaatcc
     1081 cacgtcgccg gacaggtgtt ggaactgacc aagatgacgc tgaaagattc cctttgggaa
     1141 ccgcgccgcc attccgacat ccatacgctg gaaacttccg ataatgcccg catccgcctg
     1201 aacacgaaag atgaaaaact gaccgtccat aaagcgtatc agggcggcgc ggatttcctg
     1261 ttcggctacg acgtgcggga gtcggacgaa cccgccctga cctttgaaca aaacgtcagc
     1321 ggaaaatcca atgtggtttt ggaacgccgg ccggaaaatc tgaaaacgct cgacgggcgc
     1381 aaactgattg cggcggaaaa ggcagaccct aattcgtttg cgtttaaaca aaattaccgg
     1441 cagggactgt acgaattatt gctcaagcaa tgcgaaggcg gattttgctt gggcgtgcag
     1501 cgtttggcta tccccgaggc ggaagcggtt ttatatgccc aacaggctta tgcggcaaat
     1561 actttgtttg ggctgcgtgc cgccgacagg ggcgacgacg tgtatgccgc cgatccgtcc
     1621 cgtcaaaaat tgtggctgcg cttcatcggc ggtcggtcgc atcaaaatat acggggcggc
     1681 gcggctgcgg acgggcggcg caaaggcgtg caaatcggcg gcgaggtgtt tgtacggcaa
     1741 aatgaaggca gccgactggc aatcggcgtg atgggcggca gggccggcca gcacgcatca
     1801 gtcaacggca aaggcggtgc ggcaggcagt tatttgcatg gttatggcgg gggtgtttat
     1861 gctgcgtggc atcagttgcg cgataaacaa acgggtgcgt atttggacgg ctggttgcaa
     1921 taccaacgtt tcaaacatcg catcaatgat gaaaaccgtg cggaacgcta caaaaccaaa
     1981 ggttggacgg cttctgtcga aggcggctac aacgcgcttg tggcggaagg cgttgtcgga
     2041 aaaggcaata atgtgcggtt ttacctgcaa ccgcaggctc agtttaccta cttgggcgta
     2101 aacggcggct ttaccgacag cgaggggacg gcggtcggac tgctcggcag cggtcagtgg
     2161 caaagccgcg ctggcattcg ggcaaaaacc cgttttgctt tgcgtaacgg tgtcaatctt
     2221 cagccttttg ccgcttttaa tgttttgcac aggtcaaaat ctttcggcgt ggaaatggac
     2281 ggcgaaaaac agacgctggc aggcaggacg gcgctcgaag ggcggttcgg cattgaagcc
     2341 ggttggaaag gccatatgtc cgcacgcatc ggatacggca aaaggacgga cggcgacaaa
     2401 gaagccgcat tgtcgctcca atggctgttt tgatgcgtcg ggaaatgttt tgacgcacag
     2461 gcggcacacc ggcacggccc cgcgcgccgc cccgcaaacc aatccgaacc ctgccgcccc
     2521 gaagggcggg gcatcatgat gaaaccggcg gaaaaccgcc ggttttttgc cgccgtttga
     2581 aacccgattc tggcttcaga cggcattgtc gcggcatcgg gcggcagggt ttggaacagc
     2641 ggcataaaaa actgatacaa tccgccgatt gataatggtt attttttatt tttgtgggaa
     2701 gacatttatg cctgcacgaa acagatggat gctgctgctg cctttattgg caagcgcggc
     2761 atatgccgaa gaaacaccgc gcgaaccgga tttgagaagc cgtcccgagt tcaggcttca
     2821 tgaagcggag gtcaaaccaa tcgacaggga gaaggtaccg gggcaggtgc gggaaaaagg
     2881 aaaagttttg cagattgacg gagaaaccct gctgaaaaat cccgaattgt tgtcccgcgc
     2941 gatgtattcc gcagtggtct caaacaatat tgccggtatc cgcgttattt tgccgattta
     3001 cctacaacag gcgcagcagg ataagatgtt ggcactttat gcacaaggga ttttggcgca
     3061 ggcagacggt agggtgaagg aggcgatttc ccattaccgg gaattgattg ccgcccaacc
     3121 cgacgcgccc gccgtccgta tgcgtttggc ggcggcattg tttgaaaaca ggcagaacga
     3181 ggcggcggca gaccagttcg accgcctgaa ggcggaaaac ctgccgccgc agctgatgga
     3241 gcaggtcgag ctgtaccgca aggcattgcg cgaacgcgat gcgtggaagg taaatggcgg
     3301 cttcagcgtc acccgcgaac acaatatcaa ccaagccccg aaacggcagc agtacggcaa
     3361 atggactttc ccgaaacagg tggacggcac ggcggtcaat taccggctcg gcgcggagaa
     3421 aaaatggtcg ctgaaaaacg gctggtacac gacggcgggc ggcgacgtgt ccggcagggt
     3481 ttatccgggg aataagaaat tcaacgatat gacggcaggc gtttccggcg gcatcggttt
     3541 tgccgaccgg cgcaaagatg ccgggctggc agtgttccac gaacgccgca cctacggcaa
     3601 cgacgcttat tcttacacca acggcgcacg cctttatttc aaccgttggc aaaccccgaa
     3661 atggcaaacg ttgtcttcgg cggagtgggg gcgtttgaag aatacgcgcc gggcgcgttc
     3721 cgacaatacc catttgcaaa tttccaattc gctggtgttt taccggaatg cgcgccaata
     3781 ttggatgggc ggtttggatt tttaccgcga gcgcaacccc gccgaccggg gcgacaattt
     3841 caaccgttac ggcctgcgct ttgcctgggg gcaggaatgg ggcggcagcg gcctgtcttc
     3901 gctgttgcgc ctcggcgcgg cgaaacggca ttatgaaaaa cccggctttt tcagcggttt
     3961 taaaggggaa aggcgcaggg ataaagaatt gaacacatcc ttgagccttt ggcaccgggc
     4021 attgcatttc aaaggcatca cgccgcgcct gacgttgtcg caccgcgaaa cgcggagtaa
     4081 cgatgtgttc aacgaatacg agaaaaatcg ggcgtttgtc gagtttaata aaacgttctg
     4141 attgctgttc cttttcggag gaaaccctgc cggcggcggt atcacggcgg gcatcggcgg
     4201 ctttcgggcg gtgctttgcg tgccgccgcg tgtgcggaaa cgcattccgg tttttccggc
     4261 ataacggcga tgcgaggtaa aatgccgtct gaaacccgat tcgggcttca gacggcattg
     4321 tcgcggttgc ggcgggcggg ttcaccagat tccgtcaaag gttttcgcgc cgcgccaaaa
     4381 tttccacctg tcggcgggtt tgaaggtcag cgtaccgccg tgttgtccgt ccgtggcgat
     4441 gtccagccgt ttgattttgc cgatgcggac ggcttcgtaa agcggtgcga accagcgttc
     4501 ttcccactgc tgcaatattg ccgcataccg ctccctgtcc cctgtcaggg cggtcaggcg
     4561 caaatcgtcc ataaacagga tatggtgcgt gtcgggcagg tgtgccgccg tttcttcata
     4621 ggcgcggaag ttgtcgggca atgcgcggcg gtcggggtgg aaacggctcc aaaccgtatc
     4681 ggcgaaaagc gtgccgcctt gcgtgccgcc gtttgtgccg ccccaaagcc ataagccgtt
     4741 caactcgggc agcccgcgtt tcttgcggtt atggttgacg gggtgcgccg ccagccacat
     4801 ttggatttcg gtttggacgc gcagccattc caacgcatct tctccgtccg gctgatcgtc
     4861 cgcgcccaac aatccgccca agtccaaaac gggcttcgcg ccccagcggt acgcgcgggg
     4921 aagggaaacc agccataatt cgggcaggac gggaacgaaa cgccacggaa tatcgccgta
     4981 aaacgccgac agatcgcggc agagccgttc cgcttcatcc gtaccgacat ccaaatattc
     5041 cgccgtcagc acatttgcct gatgcatccc catcttttgc cagacgggcg tggcgagcgc
     5101 gacggtttca gacggcatat tcaggctttg cgccgcgcgt tccgccagtc tgccgcacca
     5161 caaataacgc gcgtaaaatg ccgaagccgt gcagctttgg cggtgcagcg agccgtattg
     5221 caggattttg ttgaaggcgg gcaggcatag aggtattcgg atttcgtctt catccaaatt
     5281 gagcgagggg agggcgaggg tgagtttcat cgtttgatgt ctgacaaatg cggatcgggc
     5341 ggatattata gaggattcgg cgcaaacgcc gtcaaaaagg aacaatatgg ctgtcttccc
     5401 actttcggca aaacatcgga aatacgcgct gcgcgcgctt gccgtttcga ttattttggt
     5461 gtcggcggca tacattgctt tgacagagag gacggaacgc gtcagaccgc agcgcgtgga
     5521 acaaaatctg ccgccgctgt cttggggcgg cagcggcgtt cagacggcat attgggtgca
     5581 ggaggcggtg cagccgggcg actcgctggc ggacgtgctg gcgcgttcgg gtatggcgcg
     5641 ggacgagatt gcccgaatca cggaaaaata tggcggcgaa gccgatttgc ggcatttgcg
     5701 tgccgaccag tcggttcatg ttttggtcgg cggcgacggc ggcgcgcgcg aagtacagtt
     5761 ttttaccgac gaagacggcg agcgcaatct ggtcgctttg gaaaagaaag gcggcatatg
     5821 gcggcggtcg gcttctgagg cggatatgaa ggttttgccg acgctgcgtt cggtcgtggt
     5881 caaaacgtcg gcgcgcggtt cgctggcgcg ggcggaagtg cccgtcgaaa tccgcgaatc
     5941 cttaagcggg attttcgccg gccgcttcag ccttgacggt ttgaaggaag gcgatgccat
     6001 gcgcctgatg tacgacagcc tgtatttcca cgggcagcag gtggcggcgg gcgatatttt
     6061 ggcggctgaa gtcgttaagg gcggcacaag gcatcaggca ttctattacc gttcggacaa
     6121 ggaaggcgga gggggcggca attattatga tgaagacggc agggtgttgc aggaaaaagg
     6181 cggcttcaac atcgagccgc tggtctatac gcgcatttct tcgccgttcg gctaccgtat
     6241 gcaccccatc ctgcacacat ggcggctgca cacaggcatc gattatgccg caccgcaggg
     6301 aacgccggtc agggcttccg ccgacggcgt gattaccttt aaaggccgga agggcggata
     6361 cggcaacgcg gtgatgatac gccacgccaa cggtgtggaa acgctgtacg cgcacttgag
     6421 cgcgttttcg caggcggaag gcaatgtgcg cggcggcgag gtcatcggtt ttgtcggttc
     6481 gaccgggcgt tcgaccgggc cgcacctgca ttacgaggcg cgcatcaacg ggcagcccgt
     6541 caatcctgtt tcggtcgcat tgccgacacc ggaattgacg caggcggaca aggcggcgtt
     6601 tgccgcgcag aaacagaagg cggacgcgct gcttgcgcgc ttgcgcggca taccggttac
     6661 cgtgtcgcaa tcggattgaa gtttgaaccg gcgacgaaaa caatgccgtc tgaaaacctg
     6721 caaacaggtt ttcagacggc atttatagtg gattaacaaa aatcagtacg gcgttgcctc
     6781 gccttagctc aaagagaacg attctctaag atgctgaagc accaagtgaa tcggttccgt
     6841 actatttgta ctgtctgcgg cttcgttgcc ttgtcctgat ttttgttaat ccaccataca
     6901 gtcgattaaa ataaaactaa gccaaggaag cactgccgtc attcccgtac cgtcgggaat
     6961 ccggacacca cggtacggaa acccatccgc cgtcattccc gcgaaagcgg gaatctggaa
     7021 atacaacgcg gcaggagttt atcggaaatg atggaaaccc aacgcaccgg attcccactt
     7081 tcgcgggaat gacgaagtag gtaggaatcc ggatttatcc gttccgacag tgtttgcaaa
     7141 taaaagaaaa cccaaccgtc ccgattcccg gcagggctgt gttacggatt ttgcagcgag
     7201 ggcgcggggc ggtcttgcgc ctgtttggtt tgcagggtcg tcagtttttt cgtcagcaga
     7261 ttcagaatca cgccgtaggc gggcaggaaa aacagaccgc agacggtgag tttgaacagg
     7321 taatcgacaa aagcgatgcc ctgccagttt gccgccataa attcatcgct gcttgcatag
     7381 aaggcaacgg cgaaaaatac caacgtatcc aaggcgttgc cgatgacggt tgatgcggtt
     7441 ggggcaatcc accacgcttt cagacggcgt aatttgttga atacaaaaat atcaaggatt
     7501 tgtccgagcg cataggcggc aaagctggct aaggcgatgc gtccgacaaa ggtgttgaat
     7561 tcggacagcg cgcccaagcc tgtccaactg ccgttgtgga acaaaacgga aaagacgtag
     7621 gaaagcaaaa gggcggggaa catcacccaa aagataatcc gccgtgccaa gtgcgaaccg
     7681 aaaatgcgga cggtcaggtc ggtggcaagg aagatgaagg gaaaggaaaa tgcgccccaa
     7741 gtggtgtgga tgccgaaaat ttggaagggg aactgcacca gatagttgct ggcggcgatg
     7801 atgaggatat gaaaaagcac cagccggaag agtgccttct gttgctgtgc gtcggtaaag
     7861 gcgtacataa aaaaaccttt cggaaaggcg ttcagacggc atatcgtatc gaaggaatgc
     7921 cgtctgaaat atgggaagga tggtttattg tgcgtcgtgc tcaaacaggc gtttacgcgc
     7981 caatgcttca aactcggtgc ctgctttgcc gtaattggcg aaaggatgaa tggcgatgcc
     8041 gccgcgcggt gtgaactcgc cgaatacttc gatgtatttc ggatccatca gggcaatgag
     8101 gtctttcatg atgatgttga cgcagtcttc atgaaaatcg ccgtggttgc ggaagctgaa
     8161 gaggtagagt ttcagggatt tgctttccac cattttgatg tgcggaatgt agcggatgta
     8221 gatggtggcg aagtcgggct gcccggtcat ggggcagagg ctggtgaact cgggacagac
     8281 gaatttgacg aaatagtcgt tgtcgggatg tttgttgtcg aatgcttcga gaatttcagg
     8341 cgcgtagccg gtcggatatt gggttttttg attgcccaaa agagagatgc cttgcagctc
     8401 ttcgttgttg cgggacatga gggtttcctt agttttttaa tgtgggaggt tttcgaacca
     8461 cgggcggcga ttgtaatata agtggcggta tttgtgtagt tttcttcggt tagtatgggt
     8521 aattgcttta ctcggtgatg tgtggatata aatagttctg tgaggccggg ctgatacagt
     8581 ttatttgaat attcattcta aatcattaac ttgattgagt tattgccagg gtatagaatc
     8641 ctaaccaatc tttaattggt tttatttat
//
LOCUS       DAXHNE010000081         8621 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.081, whole genome shotgun sequence.
ACCESSION   DAXHNE010000081 DAXHNE010000000
VERSION     DAXHNE010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8621
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.081"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            106..354
                     /locus_tag="ACFM4Z_001361"
     CDS             106..354
                     /locus_tag="ACFM4Z_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFC6021389.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLIGIEKP
                     ARNAGRLKKFSHMENTDISRILEDDNETAALFFEESAL"
     gene            351..737
                     /locus_tag="ACFM4Z_001362"
     CDS             351..737
                     /locus_tag="ACFM4Z_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFC6021390.1"
                     /translation="MRKILLDTHALLWWLLDDKKLGISARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(917..2272)
                     /gene="yegQ"
                     /locus_tag="ACFM4Z_001363"
     CDS             complement(917..2272)
                     /gene="yegQ"
                     /locus_tag="ACFM4Z_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFC6021391.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVADMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQECPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLTGHSTAKQSQYVGHVTEIDENGWATVEVKNR
                     FAVSDSLEIIHPSGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPNMQGKEKALIARVLN
                     P"
     gene            2597..3385
                     /locus_tag="ACFM4Z_001364"
     CDS             2597..3385
                     /locus_tag="ACFM4Z_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFC6021392.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAEAGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALKHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEREGLKTVRWHEEAVDLLPAGASKTDGIRSVVEALGWEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPSVDEDGVLRGLQDLGVI"
     gene            complement(3431..4030)
                     /locus_tag="ACFM4Z_001365"
     CDS             complement(3431..4030)
                     /locus_tag="ACFM4Z_001365"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFC6021393.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKLGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            4158..4775
                     /gene="gmk"
                     /locus_tag="ACFM4Z_001366"
     CDS             4158..4775
                     /gene="gmk"
                     /locus_tag="ACFM4Z_001366"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFC6021394.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTGAEGVNALAAAGYDVIL
                     EIDVQGAAQVRDALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            4834..5040
                     /gene="rpoZ"
                     /locus_tag="ACFM4Z_001367"
     CDS             4834..5040
                     /gene="rpoZ"
                     /locus_tag="ACFM4Z_001367"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFC6021395.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            5132..7288
                     /locus_tag="ACFM4Z_001368"
     CDS             5132..7288
                     /locus_tag="ACFM4Z_001368"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC6021396.1"
                     /translation="MPAPQPSAPYDPLTAEARALLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(7355..8539)
                     /gene="coaBC"
                     /locus_tag="ACFM4Z_001369"
     CDS             complement(7355..8539)
                     /gene="coaBC"
                     /locus_tag="ACFM4Z_001369"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFC6021397.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILKGKKVLITAGATFEAIDPVRGITNISSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAENMHRAVHRLIDKQDAFISVAAVSD
                     YRVKNRSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMTYAR
                     EKRIKKKLPMIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMRIVERLAVYL
                     SK"
BASE COUNT         2145 a         1992 c         2324 g         2160 t
ORIGIN      
        1 caaggtgtga ataaaacggc ggatataatc aaatatatgt aatgagattc ccaaaatctt
       61 ggtaatgatg tatattagtc aagttagcta atcatgagaa gtgttatgtt tcaagcaaat
      121 attcatcagg caaaaaccaa tttgagtcaa ttgattcaaa gagcagaagc aggggaaatc
      181 gttattattg cgaaggcagg taagccttgc gtccaactaa tcggtattga aaaaccggca
      241 cgaaatgcag gaaggttgaa aaaattcagt catatggaaa atacggatat ttcacgtatt
      301 cttgaggatg acaatgaaac ggcagcttta ttttttgagg agtcggctct gtgagaaaga
      361 ttctgcttga tacccatgcg ttgctgtggt ggttgttgga tgacaagaaa ctggggatat
      421 ctgcacgcaa actgatagaa aatccgagaa atgcgatctt tgtcagtgca gcaagtattt
      481 gggaaatttc catcaagcag aacaaggggt tgttgaaatt accggaagag ttttttgatg
      541 tgcttcaaga agaggatttt gaaatgttgc ctataggtct gtttcatgca aaacaggctg
      601 gaagccttcc ggagatacac aaagaccctt tcgataggat gttgattgca caaacccaag
      661 cggaaggctt tgaactgatg actgtcgatg aatatattcc gcaatatgga attagagttg
      721 tcaacgcatc aagctgacaa atacaaaaac tgtgtacaca agaaagaccg ccgaggcggt
      781 cttttcggtt attgtcttcg cttaaaagcc agacttctgt cgtggactat aggtgtgtcc
      841 cgatctgaat ctgccgcaca ataaggcaag gggattaaaa atgccgtctg aaaaatgttt
      901 cagacggcat aatggcttag gggttcaaca cgcgtgcgat gagggctttt tctttaccct
      961 gcatattggg gattttgacc tgaatgccgt tgcccggggc aacatcgaca ggctggcctt
     1021 tgcgggtcat ttgttccaat ttgatggttt ggttgccgct cgggtggatg atttcgagtg
     1081 aatcgctgac ggcaaagcgg tttttgactt ccactgttgc ccagccgttt tcatcgattt
     1141 cggtaacgtg tccgacgtat tggctttgtt tggcggtgga atggccggtc aggtagtttt
     1201 gataatcctg agtttggtgg cgttcgagga agccgctggt gtagccgcgg ttggcgaggc
     1261 cttcgagttc gctcaacagg ctgtaatcaa acggacggcc tgcgacggca tcatcaatcg
     1321 ctttgcggta ggactgggcg acgcgtgcaa cataatagag cgatttggta cggccttcga
     1381 ctttgaggct gtccacgccg attttggcga gtttttcgac gacttcgata ccgcgaaggt
     1441 ctttggaatt catgatgtag gtgccgtgtt cgtcttccat aatcggcatc atttcgcccg
     1501 ggcggttgga ttcttcaatc aggaaaactt tgtcggcgta gggatggcgt ttttgaccgt
     1561 tgatgccttc aaagttttgg ttggcttctt cttgggcttt ttcaaagttg aaaccttgca
     1621 gaagctgggc atcgcctgca tcgctttccg tggcattgtg aaccttgtaa tcccaacggc
     1681 aggagttggt gcaggtgcct tggttggggt cgcggtggtt gaaatagccc gacaataggc
     1741 aacggcctga ataagcgatg cacaatgcgc cgtggatgaa gacttcgagt tcgatgtcgg
     1801 ggcattcttg gcggatttcg gcgatttctt ccatactcaa ttcgcgcgac agaataatgc
     1861 gttcgacgcc gatgttttgc cagaatttca cgccccaata gttggtggtg ttcgcctgta
     1921 cggacagatg gatcggcatt tccggccatt tttcgcgcac ggtcataatc aaacccggat
     1981 ccgccataat cagcgcgtcg ggtttcatgg caatcagcgg ctccatgtcg gcaacgaagg
     2041 ttttgagttt ggaattgtgc ggcagggtgt tgacggttaa aaagaatttt ttgttgcgct
     2101 cgtgcgcttc tttaatgcct tgttctaaaa catcaagttt ggcaaattcg ttgttgcggg
     2161 cgcgcagtga gtaacgcggg ctgccggcgt aaacggcgtc tgcgccgtag tcgtaggcgg
     2221 cgcgcattct ttccaatccg ccggcgggca ataagagttc gggtgctttc atcgtgtttc
     2281 cttttcggtt gaaaccccgc cctttagggc agtagaatca gactttattt gggaggggcg
     2341 taaccccttc cgaatcaggg caacatatag ggcgacgctt tatgtgtcgt cctgtgtgtt
     2401 gaaacatgat ttgtatgctt tgtaaaaatg tttcagacgg cattgctgca aatgccgtct
     2461 gaaaagggtt tgaggatggg cggattatcc gcctttttgt attttcggtc aattttgacg
     2521 ggaaatttat aaggatttat caagtatttg cctgcttggg atataatgcg gtagtttgaa
     2581 tgcgagagag aatgtgatga atcctaaaat cgtgtttttc gacattgacg atacgctgta
     2641 ccgaaaatat acggatactt tgcgcccttc cgtgaaaacg gcggtggcgg ctttgcgcgg
     2701 caaaggtata ttgacggcgt tggcaacggg gcggtctttg gcgacgattc ccgaaaaggt
     2761 cagggatatg atggcggaag cgggaatgga tgccgtggta acgataaacg gacagtttgc
     2821 gctgctgcac ggaaaaaccg tgcgcgaagt accgatggat gccggtttga tgggcagggt
     2881 ttgcgcgcat ttggatggct tgggcatgga ttatgcgttt gtcggcggag aggggatcgc
     2941 tgtgtccgcg ctgtcggaat gcgtgtgccg cgccttgaag catatcgcca gcgatttttt
     3001 tgccgataag gattattttt caagcaaacc ggtgtatcag atgctggtgt ttgcggagga
     3061 aaacgaaatg ccgctttggt cggatattgt ggaacgggaa ggcttgaaaa cggtgcgctg
     3121 gcacgaggaa gcggtcgatc tgctgcctgc gggtgcgtcg aaaacggacg gcatcagaag
     3181 cgtggttgaa gcattgggat gggaaatggc agacgtgatg gcgttcggcg acggtttgaa
     3241 cgatgtggaa atgctgtcag aagtcgggtt cggcgtggca atgggcaacg gggaacaggc
     3301 ggcgaaagaa gcggcgaaat atgtttgccc cagcgttgat gaagacggcg tgttgagggg
     3361 cttgcaagat ttgggcgtga tttgaacgca tcataacaac cctgccggtt tcagacggca
     3421 gggtcggttt tcagcccttc atacagcctt cgttttgaag cagggtaaat aagggcgcgc
     3481 cgcttgcacg gatattcttg ccgccttgaa ggtcggtaaa ttccaaaatg gcggcggctt
     3541 cgacaatttc tccgccgagt ttgcggatca gttccagtcc ggcaagcatc gtgccgcccg
     3601 tggcaaccaa atcatcgacc agcagcacgc gcgaaccgag tttgacggca tcggtgtgga
     3661 tttccaccgc agcttccccg tattcgagcg cgtagctttg cgatacggtt tcaaaaggca
     3721 gcttgccttt tttgcggatg gggacgaaac cgacgttgag ctggtaggcg agtgccgcgc
     3781 cgataatgaa gccgcgcgcg tccaaaccgg caacgatgtc gattttctga tccatatagc
     3841 ggtaaaccaa taaatcaacc aaaaggcgga agtattccgc gctttgaagg acgggcgtga
     3901 tgtcgtggaa taagatgcct ttttgcggcc agttttcgat tttgcggatt ttgtcggcaa
     3961 gcgcgccgac actcatagct tcgggatgaa ccagcattgc gtgttccaag tttgatgttt
     4021 ttaacgccat attgtagccg tcagcctgtc gcttgccaaa tcaatcaaaa caatagcggc
     4081 aaaatatggg catcggcaaa taggaaactg ggtgtccatt gtgatacaat cggctggaaa
     4141 atatacaggt aaaaaatatg tccgcttatc gaaaaggcaa catctttatc atttcggccg
     4201 cttccggcac gggcaaaacc acgctggtgt cgcggctgtt ggcaaaccat aacggtttgc
     4261 gcgtttccgt gtcgcacacg acgcgcccgc cgcgtgaagg cgaagcaaac ggcgtacatt
     4321 atcactttgt ttccaaagaa gagtttgagt cgcttatcgc gcaggaagct tttttggaat
     4381 acgccgacgt atttggcaac tattacggca caggcgcgga gggtgtgaat gcgttggcgg
     4441 cggcaggcta tgacgtgatt ttggaaatcg acgttcaggg cgcggcgcag gttcgcgacg
     4501 cgctgcccga agccgtcggc atctttatcc tgccgccttc tttcgacgta cttgccgcgc
     4561 gcctcaacgg acgcgggacg gacagtcggg aagttatcca aaggaggctg tcgaaggcaa
     4621 ggcatgaaat cgagcagtcc gtattgtttg actttgtcgt ggtcaatgac gacttggcgc
     4681 gagcggagga ggatttgcgc catattgtga atgcctgccg tctgaaaagg tcgcggcaac
     4741 tggggtttat tgcagatttg ttggaaaatt cctagaaaac ggcgaaaata cccggtttcc
     4801 caatttaaat atttttgaaa gaaagcaaat aatatggcac gtattaccac cgaagactgt
     4861 accggaaaaa tttccaacca ttttgacctg acattggtag cggctcgccg cgcccgccag
     4921 cttgagaacg gcaacacgcc gcttgtggac gatgtccgca ataacaaacc gaccgttacc
     4981 gccttaaggg aaatcgccgc cggacatatc ggtacagaac tgttgacgcg caataaataa
     5041 attctgccgg aaacgcacgc cggaacactt tgccgccgtg cagtccgacg gtttgaaatg
     5101 aaaaacacat acataccgaa agccgtctga aatgcccgcc ccccaacctt ccgcccctta
     5161 cgaccccctg accgccgaag cgcgtgccct gcttttccat accgcctcct acctcaagcc
     5221 cgaggaacag gcggagcttg aaaaagctgt cgcctatgcg tttcgcgccc acgacgggca
     5281 aacccgcaaa agcggggagc cctacatcac gcatccgatt gccgttgcga cgcagctcgc
     5341 cctttggcat atggacatac agggtctttg tgcaggcgtg atgcacgacg tattggaaga
     5401 tacgggcgtg acaaaagggg aaatggcggc ggtgttcggc aatacgattg ccgagatggt
     5461 ggacggtctg tccaagcttg aaaaactcaa atttgaagat catgcggagc atcaggcgga
     5521 gagtttccgc aaactgattt tggcaatgac caaagatgtg cgcgtgattg tcgtcaaact
     5581 tgccgaccgc ctgcacaata tgcggacgct cggttcgatg cgcccggaca aacgccgccg
     5641 gattgcaagg gaaacccttg aaatctatgc acagattgcc aaccgtatag gtttgaataa
     5701 cgcatatcaa gagcttcagg atttatcgtt ccaaaacctg catcccaacc gctacgagac
     5761 tttaaaaaaa gcgatggaca agagccggaa gaaccggcag gacgttgtcg gcaaagtctt
     5821 gcgcgcattc ggccagcggc tggtaggcgc gaatatagag gccaaaatca aaggcaggga
     5881 aaaaaacctg tacggcatcc atcagaaaat gatggcgaaa aagctgcgct ttgccgaggt
     5941 tatggatatt tacggtttcc gcgtcattgt caacagcatt ccagcctgtt atgccgcact
     6001 cggcgcattg cacaccctct atcagcccaa gcccgggcgg ttcaaagact atatcgccat
     6061 tccgaaaagc aacgggtatc aaagtctgca tacgactttg gtcggccctt acggcttgcc
     6121 gattgaagtt cagatacgta ccaaggaaat ggatgctgtt gccgaaggtg gaatcgccgg
     6181 acattggagc tataaatcat attctaagac ggtcgatcag gcggtgcttc acacaaaccg
     6241 gtggctgaaa aatatcttag atttgcaggc aagcagtgcc aatgccattg agtttctcga
     6301 acacgtcaaa gtcgatttgt ttccgaacga aatctacatc cttacgccaa aaggaaaaat
     6361 cctaactttg cccaaagggg caacgcctgt cgattttgct tatgcggtgc ataccgatat
     6421 cgggcacaaa accgttgccg cacgtatcaa caatatcatg atgccgttgc gtacgaagct
     6481 caaaaccggt gattctgttg aaattatcac atccgaacac gccaaaccca atcccgcgtg
     6541 gttgaatttc gccgtgtcag gcagggcgcg cagcgccata cgccaatata ttaaaaacct
     6601 taaccggcac gatgcggtcg ttttgggaga gagcctctta caaaaagccc tgtccagttt
     6661 gctgcccaaa gatgtcctgc tttcagacgg catcaaggaa aaatatcttg ccgatctcaa
     6721 cgacaagcag acatcgtttg aagaagtgct gtacaacgta gggatggggc ataccctgcc
     6781 tgtttatgtc gccatgcaca ttgccgagtt ggcaggggag catttcggca gcgaggtcag
     6841 gctcagttcc attaaagtcg atgggcagga aagcgggcat attcattttg cagagtgctg
     6901 ccaccctgtt cccggcgatt ccatccgttt gctgttggtt aagggaaaag gcatgattat
     6961 ccatagggat acctgcccga cgttgttgaa gtccgatccc gaacagcagc tggatgcaga
     7021 ctgggaaaat atgaacgggc agaactaccg tgtcgggctt caagtccaat cggaagacag
     7081 ccacggcctg ttggcattaa tggcgcaagc gatttccgat tccggtgcag acattgagtc
     7141 ggtcgaaaca ccgtctaaat cccagtcggg aacggaaggt tttgtcgaat tcaaattctt
     7201 attgaaagtc aagaatctga atcaattgaa tcagattatt caaaatctgc atagtattcc
     7261 atatatccgc aaagtcatca gaagttgaag caggtaaaac gggttgcctt ttgattaaca
     7321 ggcaacccgt tttatggttt atccgttcaa ttgcttattt gctcaaatat acggcaagcc
     7381 tttcaacaat ccgcattgcc gcttcatctt tacttgtttc gggaaaagac agttccccgt
     7441 cgtccccgat aatggtaatc cggttggtcg gtttgcccat tgcgattgaa acatcattgg
     7501 caacgatcat cggtagcttt ttcttaatac gtttttcccg cgcatatgtc attacattct
     7561 ccgtttcagc ggcaaaaccg atgcagaacg gcgggttcgg taatgaggca atagaagcca
     7621 aaatatcggg gttctcatcc aattcgatgg ataacggttt ggcattttta tcttttttga
     7681 atttttgagt actcctattc ttaaccctat agtctgagac ggcggcaaca gaaataaaag
     7741 catcttgttt gtcgattaaa cgatgcactg cgcgatgcat attttcggca ctgacggctt
     7801 gaaccgtatc ggatatgccg aaaggcagcg cggtttgaag ctgtccgtga atcaggctga
     7861 cttctgcacc ggcggcacgg cacgcccgcg ccaaagccac gcccattttc ccgctggaga
     7921 tatttgtgat gcctcggaca gggtcaatgg cttcaaatgt cgcacctgcg gtaatcaaga
     7981 cttttttgcc ctttaaaatt ttcggtgtcc ataaatccgg aagcagatcc agcaattcgg
     8041 caggttccgg catccttccc ataccatttt ctccgcaagc ctgttcgccc aagcccggca
     8101 tatagacagt aatgccgtct gaaaccagtt gtgcgatatt ccgttggttg gcagggttga
     8161 gccacatttc cacattcatc gcgggcgcga tggcaagcgg acatttccgt gcggctgcca
     8221 gactggtcag taggttatct gccacgccgt tacagatttt tgccacggta ttcatacttg
     8281 ccggcgcaat cagaaaaaca tccgcattcc gggtcaggtt gatatgttcc ataccgtttg
     8341 aaccgttgcc gccgtgcgtg tcggtcagga caggattgcc gcttaaagcc tgaaaagtca
     8401 gcggagaaac aaattcagtt gccgagcggc tcataaccac cgtaaccgaa tgcccctgtt
     8461 ttttcagcag tcgcaccaac tcgcaagact tatacgccgc aatactgccc gttacaccta
     8521 aaagaatatg tttgcccatt ttgacaattt ctaattacga agaaaaaaca gcagccattc
     8581 tataacaaag tacggataca ttagataccg tttggataca a
//
LOCUS       DAXHNE010000082         8350 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.082, whole genome shotgun sequence.
ACCESSION   DAXHNE010000082 DAXHNE010000000
VERSION     DAXHNE010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.082"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(360..1745)
                     /gene="pntB"
                     /locus_tag="ACFM4Z_001370"
     CDS             complement(360..1745)
                     /gene="pntB"
                     /locus_tag="ACFM4Z_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFC6021398.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFIGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSS
                     AASFAGQEVSEYREVKAADVAEMLKGANSVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1811..2194)
                     /locus_tag="ACFM4Z_001371"
     CDS             complement(1811..2194)
                     /locus_tag="ACFM4Z_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFC6021399.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLAHTQ
                     GAAARAHAAQQNGFEVFAPFAAAVLTAHATGNAAQSTINTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWVGGFVCTVGLFVVAA"
     gene            complement(2198..3739)
                     /locus_tag="ACFM4Z_001372"
     CDS             complement(2198..3739)
                     /locus_tag="ACFM4Z_001372"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFC6021400.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWVCPLIYKVNAPSEQELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTHDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVSLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQISQGNGFVTLLAFIAILIASINIFGGFAVTRRMLN
                     MFKKG"
     gene            4029..4862
                     /gene="serB"
                     /locus_tag="ACFM4Z_001373"
     CDS             4029..4862
                     /gene="serB"
                     /locus_tag="ACFM4Z_001373"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HFC6021401.1"
                     /translation="MPHALVLQFPSAEALPSDFPSRLPEPDCADEKRMRFIVEEGFSL
                     SGADAALLGSRQIDHAVLPDMDFGELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPMLKEAGIGVAYRAKPKARAAADACINFGGLERVRGLF
                     G"
     gene            4926..6149
                     /locus_tag="ACFM4Z_001374"
     CDS             4926..6149
                     /locus_tag="ACFM4Z_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC6021402.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGADGAY
                     ISFREGVARASEGRRLGVLILCGMLAGGGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEDEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLTV
                     NTSLLWFSAVIGPILGVAAVFFQRTAQKFPFIKRDNIKIIPLAVCMFALIGVISVWFP
                     EILGNGKAGNQLTFGGLTDWQHSLGLTAVKWLVVLMALAVGAYGGLITPSMMLGSTIA
                     FAAATAWNSVFPEMSSESAAIVGAAVFLGVSLKMPLTAIAFILELTYAPVALLMPLCT
                     GMAGAVWVAKKMGFK"
     gene            6313..7893
                     /gene="purH"
                     /locus_tag="ACFM4Z_001375"
     CDS             6313..7893
                     /gene="purH"
                     /locus_tag="ACFM4Z_001375"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFC6021403.1"
                     /translation="MSSIKRALISLSDKTGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGTPEIAGFPGRFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLAAYKQLQGKELSYNNIADADAAWEAVKSF
                     DVPACVIVKHANPCGVAIASNTLDAYKLAYATDTTSAFGGIIAFNREVDGATVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHR
                     ISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTGIRHFRH"
BASE COUNT         1881 a         2262 c         2325 g         1882 t
ORIGIN      
        1 ttgatgtttt gtatatgcac ttccacaaag ccaacgagtt tttgggtatg acccgcctgc
       61 cgacattctt atgtaacgat gtggttaaaa atccgcaagt ggaaaaatac ttggcagatt
      121 atcaggcaca cttggaaaaa gtgttcggct aattaaaaat ccatcttcaa cacggagatg
      181 gattttgttt gtttcgttga ttttgtgtca gtttcagatg taggtgctta ttcggacggg
      241 ccgtctgaaa atgtttgccc caatgcaaaa aaatcactgc aaaccttcat aaacggggtt
      301 tgcagtgatt ttttcaaatc aaacagattg aaaacctgcg ccgaattgtt cagacggcat
      361 tattttttca gttcggacag aatgtcatcc acggttttct tcgcatctcc gaaacacatt
      421 acgctgtttt cgttgaagaa cagcgggttt tgtacacctg cgtagccggt attcatcgag
      481 cgtttgaaga cgacgacttc ttttgccttc catacttcca acacgggcat acccgcaatc
      541 gggctgttcg ggtcggtttg ggcggcagga ttgacggtat cgttcgcacc gatgaccaag
      601 accacatcgg tttcggggaa atcgtcgttg atttcgtcca tttccaacac gatgtcgtag
      661 gggactttgg cttcggcaag cagtacgttc atatgaccgg gcaggcggcc ggcgacgggg
      721 tggatgccga agcgtacttc tgtgccgttt ttacgcaaaa gctcggtgat ttcggcaacg
      781 gggtattgcg cctgtgcgac tgccatgccg tagcccgggg tgatgatgac gctgtttgcg
      841 cctttcagca tttcggcaac atcggcggct ttgacttcgc ggtattcgct gacttcttga
      901 ccggcaaaag atgccgcgct gctgtcgctg ccgaaaccac cggcaattac cgagacaaac
      961 gagcggttca tggctttgca cataatgtag gacagaattg cgccgcttga gccgaccagt
     1021 gcgccggtaa cgatgagcag gtcgttggag agcataaagc ctgccgctgc ggccgcccaa
     1081 ccggagtagg agttgagcat agatacgacc acgggcatat ctgcgccgcc gatggaggca
     1141 accaagtgcc agccgaatgc gagggcaatc agggtcataa tcagcaggat gaagccgctg
     1201 ccgtcaatgc cgataaatac gagcagcaac acaaacgata cggcgagcgc cagtgcgttg
     1261 agcttgtgtt tggcgggcag ttgcagcggg ctgctgctga ttttgccgtt gagtttgccg
     1321 aatgcgacca gcgagccggt aaaggttacc gcgccgatga aaatgcccaa atacacttcg
     1381 accagatgga tggtgtgcat atcgtgcgaa acgttgcccg gctcgatgta gctgttgaag
     1441 ccgaccaaaa ccgccgccaa gccgacgaag ctgtgcagca gggcaatcag ttcgggcatt
     1501 tcggtcattt ccaccttttt ggctttgtag atgccgattg ccgcgccgat gagcatggcg
     1561 atgatgatcc agcccagtcc gtgggtattg tcggaaaaaa cagttacaaa aagggcgacc
     1621 gccataccgg cgataccgga atagcagccc tgtttggcgg tttcctgttt ggacagcccc
     1681 gccagtgaga agatgaataa aattgcggca acgatatacg ccgctgttac gagtcctgaa
     1741 gacatggaaa ttctccgatt ttcgatgatt tgttttcaat gccgtccgaa ctgttcagac
     1801 ggtatctgtt tcaagcagcc acgacaaaca gcccgacggt gcagacaaat ccgcccaccc
     1861 acatcagcga gcgcaatgcg gctttgtcgg cgatatagca ccagataaag gcgaggcgga
     1921 acaggatgaa caggcccgca agcgtgttga tggtcgattg cgccgcattg ccggttgcgt
     1981 gtgccgtcaa aacggcggcg gcaaacggtg caaagacttc aaaaccgttt tgctgcgcgg
     2041 cgtgggcacg ggcggctgcg ccttgcgtat gcgccagaaa atcgcgcgga ttgtggttgt
     2101 ctttaaaccg gaatccgccc gcttttttgg catacgccgc acaaaaaagc ggcaataggc
     2161 aggcaatcag aatacaccaa taggcgaaag tcatggctta ccctttctta aacatattca
     2221 gcatacgccg tgttaccgca aagccgccga agatgttgat gctggcaatc aggatggcga
     2281 tgaaggcaag cagggtaacg aaaccgttgc cttggctgat ttgcagcagc gcgccgacga
     2341 cgatgatgcc ggagatggcg ttggttaccg acatcagcgg tgtgtgcagc gagtggctga
     2401 cgttccaaac gacatagtag ccgatgacgc aggcaaggac gaacacgata aagtggttca
     2461 ggaatgctgc gggtgcgacc gcgccgaccc acagcaccaa tacggcggcg atgacggcgg
     2521 gcgcgagttt tttccacagg gaaacaggtt tcggctcggg cttggcggca ggcgcggctt
     2581 tttcagacgg cgtttgctgc ggctgggcgg aaacttgaat cggcggaggc gggaaggtga
     2641 tttcgccgtc gtgggtaacg gtcatgttgc ggataatcac gtcttcgaag tccaacgtga
     2701 tttcgccgtc tttgttcggg cttaacagct tggtcaggtt gaccaagttg gtggcgtaaa
     2761 gctgggaaga ctgtccggca aggcggtttg ccatgtcggt gtagccgatg attttcacgc
     2821 cgttgccggt tacggacaat tcgcccgggc gggtgagttc gcagttgccg cccgtcgccg
     2881 ccgccaaatc gacgatgacg gagccggatt tcatgctttc caccatttct ttggtaatca
     2941 gcttgggcgc gggtttgccc ggaatggcgg cggtggtgat gatgatgtcc acttctttcg
     3001 cctgctcggc aaagagcttc atctcggctg cgataaattc gtcgctcatc actttggcgt
     3061 agccgtctcc gctgccgccc gattcttgtg ggaagtcgag tttcaggaac ttgccgccca
     3121 tcgattcgat ttgttccgcc acttccaagc gggtatcgaa cgcgcgtacc actgcgccga
     3181 gcgagtttgc cgtaccgatc gccgccaaac ctgccacacc tgcaccaatc accaaaacct
     3241 gcgcgggcgg cactttgccg gcggcggtaa tttgaccggt gaagaaacgg ccgaaggcgt
     3301 tggcggcttc aattacggcg cggtagccgc tgatgtttgc catcgaagac aaagcgtcca
     3361 aagcctgcgc gcgcgaaatg cggggcacca tatccatcgc cagcgcgttc actttcttgg
     3421 cgcgcaaggc ttcgaccaaa gcctcgtttt ggcgcggcca caggaagctg acgatggttt
     3481 gaccttcgtt caaaagcggc agttcctgtt cggacggcgc gttgacctta taaatcaaag
     3541 ggcagaccca aaccgccgct ttgtcggcaa cggttgcgcc tgctgtttgg taagcggcat
     3601 cgtccaaact tgccgccaaa cctgcaccgc tttcgacaac ggtttcaaag cccagtttgc
     3661 ccagcagggc gacggtggcg ggcgtgcagg cgacgcgggt ttcgccggat aatgactcgc
     3721 gtgggatacc gattttcatc tctgaatcct ttttcgggta gtttatatgt atcgtgggtt
     3781 aaatttaaat cggggcaggg cggagcaacg ccgtaccggt ttaaaaccga ttcacttcaa
     3841 atgttaatat attttagata atccccttat aacgaatttt catcaggctg gcaatagttg
     3901 cggcattttc ccgtgttgtc cgacacatac tgccgtttgc gtttgaagtc attttccgat
     3961 tgcagtccct cccgccgtaa cattctttgc cgtttcacta tataatccgc attttttgag
     4021 ccgccgttat gccgcacgcc ctcgtcctcc aatttccctc cgccgaagcc ctgccttccg
     4081 acttcccctc ccgcctgccc gaacctgatt gcgccgatga aaagcgtatg cgttttatcg
     4141 ttgaagaagg gttttcttta agcggggcgg acgcggcgtt gcttggcagc cgtcaaatcg
     4201 accacgccgt gttgccggat atggattttg gcgaactcgg tttgattgtc agcgatatgg
     4261 attcgacgct gattaccatc gaatgcgtcg atgaaattgc ggcaggcgtg ggtttaaaag
     4321 acaaggtagc ggaaattacc gagcgttcga tgcgcggaga gttggatttc gagcagtcgt
     4381 tgcgcagccg cgttgcgctg ttggcgggat tggacgaaca gattttggcg gacgtttatg
     4441 aaaacgtttt gaagctctcg cccggtgcgg aatttttgtt ggacgaatgc aaaaggcacg
     4501 atgtgaaatt cctgctggtg tccggcgggt tcacgttttt taccgaacgt ctgcaacaac
     4561 gcctcggctt cgaataccaa cacgccaatg ttttggaaat tgaaaacggc aggttgaccg
     4621 gccgtctgaa aggcagaatc atcgacgcgc aggcaaaggc ggatttattg cgcgaatacc
     4681 gcagccgcct cggattgcag ccacatcagg tgttggcgat gggcgacggt gcgaacgata
     4741 ttccgatgct caaagaagcg ggcataggcg tggcttaccg tgccaaaccg aaagcgcggg
     4801 ccgccgccga tgcctgtatc aatttcggcg gtttggagcg tgtacgcggc ctgttcggat
     4861 aggcggatag gaaacggatg tcgtccgaaa ggttttcaga cggcatttga acggcaggaa
     4921 cgacagtggg acgcagaaaa ctttggtttg ccctggcagc agcaggcgtt atcggcggtt
     4981 tggtcggcat tgtgctgacg gaactgatgc acttcataca gcatacggca tacggttatg
     5041 gcgcggacgg tgcgtacatc tcgttccgcg aaggtgtggc ccgtgcttcg gaagggcggc
     5101 gtttaggcgt attgattctg tgcggtatgt tggcaggcgg cggctggtgg ttgctcaaac
     5161 gtttcggtaa gccgcaaatc gaaatcaaag ccgccttgaa gcagccgttg caggggctgc
     5221 cgtttctgac gacggttttc catgttctgc tgcaaatcat aacggtcgga ctcggttcac
     5281 cgctcggacg ggaagtcgcc ccgcgtgaaa tgactgccgc gttcgctttt gccggcggca
     5341 ggcggttggg tttggatgaa gacgaaatgc ggctgctgat tgcttgcgcc tcgggtgcag
     5401 gtttggcggc cgtgtataac gtaccgctcg cctccacact tttcattctc gaagccatgc
     5461 tgggcgtgtg gacgcagcaa gccgtcgccg ctgcattgtt aacttcagtc atcgccaccg
     5521 ccgtcgcgcg catcggcttg ggcgacgtgc agcaatatca tccggccaac cttaccgtca
     5581 atacttcatt actttggttt tccgccgtca tcggcccgat actgggcgta gccgccgtct
     5641 ttttccagcg taccgcccaa aagttcccct ttatcaagcg cgacaatatc aaaattattc
     5701 ccttggccgt ctgtatgttt gcactcatcg gcgtgatttc cgtttggttt cccgaaattt
     5761 tgggcaatgg caaagcaggc aatcaactga cctttggcgg attgaccgat tggcaacaca
     5821 gccttgggct gaccgccgtc aaatggctgg tcgtcttaat ggcgcttgcc gtcggcgcat
     5881 acggcggtct gattaccccg tccatgatgc tcggcagtac catcgccttt gctgctgcca
     5941 ccgcgtggaa cagtgttttt cctgaaatgt cctctgaaag cgcagccatt gtcggcgccg
     6001 cagttttcct cggtgtttcc cttaaaatgc ccctgaccgc catagccttt attttggagc
     6061 tcacctacgc ccctgttgcc ttgctcatgc cattatgtac aggcatggca ggtgcagtat
     6121 gggtggcaaa gaaaatggga tttaaatagg caaaagcaaa aggccgtctg aaaccaagtt
     6181 tcagacggcc ttttacaata aaattgttaa caatatttgc aaaaacctac tgccaaaaat
     6241 gcgaaactgg gggataatac cgccctgaaa attcatccca tactgattaa accttcaaca
     6301 aaggaaatcc aaatgtcttc catcaaacgc gccctgatca gcctatccga caagacaggc
     6361 gcagtcgaat ttgcccaaac cctgcacaaa ctcggtgtcg aaattctttc taccggcggt
     6421 acagcaaaac tcttggctga tgcaggcgtt cccgttatcg aagttgccga ctataccggt
     6481 tttcccgaaa tgctcgacgg ccgcgtgaaa accctgcatc cgaaaatcca cggcggtatt
     6541 ctcggtcgtc gcgatttgga cgaacacgtc gccaagatgg aagaacacgg catcggcaat
     6601 atcgacctcg tgtgcgtcaa cctctacccc ttcgctgcca ccatcgccaa accaaactgc
     6661 acgctggaag acgcgattga aaacatcgac atcggcggcc cgaccatggt gcgctctgcc
     6721 gcgaaaaact ggaaacacgt cgccatcgtt accgacaccg ccgatttccc ggccatagct
     6781 gccgaactcg aagccaacaa cggcgcattg agcgacaaaa cccgtttcaa cctctcgcgc
     6841 aaagcattca gccataccgc ccaatacgac ggtatgattt ccaattacct gacctcgctt
     6901 tcagacgacg tcttgagcgg cacgcccgaa atcgccggat tccccggccg gttcaatcaa
     6961 agctggatta aagtgcaaga catgcgctac ggcgaaaacc cgcatcagcg cgccgcgttc
     7021 taccgcgata ttgaccccgc cgcaggcagc ctcgctgcat acaaacaact gcaaggcaaa
     7081 gaattgtctt acaacaacat cgccgatgcc gatgccgcat gggaagccgt caaatccttc
     7141 gacgtgcccg cctgcgtgat tgtgaaacac gccaatccgt gcggcgtagc catcgcctcc
     7201 aataccttgg atgcctacaa actcgcctac gccaccgaca ccaccagcgc gttcggcggc
     7261 atcatcgctt tcaaccgcga agttgacggc gcaaccgtca aacaaattac cgacaaccag
     7321 tttatggaag tcctcatggc gcctaagttc accgccgaag ccctcgaaat cgccgccgcc
     7381 aagaaaaacg tgcgcgtatt ggaagtgccg cttgaggcag gcgcaaaccg cttcgaactc
     7441 aaacgcgtcg gcggcggact gttggtgcaa acgcccgaca tccaccgcat cagccgcgcc
     7501 gatttgaaag tcgtctccaa acgccaaccg accgagcagg aatggaacga tttgctgttc
     7561 gtctggaacg tcgccaaata cgtcaaatcc aacgccatcg tattcggcaa aggcggtcaa
     7621 acctacggca tcggcgcagg ccaaatgagc cgcgtggaca gcacccgcat cgccgcccgc
     7681 aaagcgcaag atgccggtct cgacctcaac ggcgcgtgtg ccgcatccga tgccttcttc
     7741 cccttccgcg acggcgtgga cgtgattgcc gaacagggca tcaaagccat catccatccg
     7801 gcaggctcga tgcgcgatca ggaagttttc gacgcagccg acgaacacgg catcgccatg
     7861 gtcgtaaccg gcatccgcca tttccgccat tgatgcagat aaacaaggta atgccgtctg
     7921 aagggctttc agacggtatt ttgcgctatt ttgcgaaggt agggatgacg gttcgggtat
     7981 tcctgacagg gtggattttc aaggtgttgt atagggtgta ggaggattcg taaaaggtgg
     8041 gatgcagggt gtgcttcagc ccgctgcatc aaaaattttt ggagaaccgg cgggagtcgg
     8101 cggttttggt ttcggcgggg acggtggaaa tgggtaacat tgacggaatc gacggaagcg
     8161 gtgggctgaa gcccaccctt gtatattgga atcactgtat catagcaaca aaccgcccga
     8221 ccgccacccg cgcccaccca aggcacacaa ccgttgcgta gcacagggag cggcagggca
     8281 acccatcgac acaaccggac agttgcccga caacacaacc gaatgcaagg caggttgatg
     8341 atgagtaccc
//
LOCUS       DAXHNE010000083         8322 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.083, whole genome shotgun sequence.
ACCESSION   DAXHNE010000083 DAXHNE010000000
VERSION     DAXHNE010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8322
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.083"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(138..1271)
                     /locus_tag="ACFM4Z_001376"
     CDS             complement(138..1271)
                     /locus_tag="ACFM4Z_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021404.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNAFVAKLARLFRNADRAVVIVKESMRTEESLAGAVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGKEVWLDYHIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDAAGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     IREEKQGDKLPDFPLNLENLEKEVSRYAEAAARRSGDKRGLSY"
     gene            complement(1391..1885)
                     /locus_tag="ACFM4Z_001377"
     CDS             complement(1391..1885)
                     /locus_tag="ACFM4Z_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021405.1"
                     /translation="MKNRFAKIVKDIFSFTQFVSEAENKIPNQSSRYYDCWRELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKHLFAEEVPENNIVCDISHFLHFADWA
                     EADIPNQSRLYAEYWWEMEIFKMSVLEDWERSGKPLDWKKWQRQYRSEAFELVKKLNR
                     SKQE"
     gene            complement(2034..2369)
                     /locus_tag="ACFM4Z_001378"
     CDS             complement(2034..2369)
                     /locus_tag="ACFM4Z_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021406.1"
                     /translation="MMADKEQVEKMIYSENKQSVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLNIARLSLTCFGHLARMHENIGDCDKVIALLRSKQGNPDFQGFAEDALDDI
                     SFFIFKKHP"
     gene            complement(2381..2740)
                     /locus_tag="ACFM4Z_001379"
     CDS             complement(2381..2740)
                     /locus_tag="ACFM4Z_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021407.1"
                     /translation="MASYVSIKGWIECSDDDIKIIQENINNFWNNCPFNIEEKESAKI
                     YKSGWIFPTNSFNWSSYIFFGACVKSYFIIYFEKCIKTIMELDIEISGFFELDYYEDS
                     YKVNWKINNGNLIQTTN"
     gene            complement(2961..3416)
                     /locus_tag="ACFM4Z_001380"
     CDS             complement(2961..3416)
                     /locus_tag="ACFM4Z_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021408.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITQYKNILETFMYEPPDEF
                     GTEYYESPDSNLLISVEKNKIISIFCYQELYFMGVNIIGLNFEIFKQLFHYPKLNDID
                     KCYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIIAGGKDNYSIEPYYDE"
     gene            complement(3604..4026)
                     /locus_tag="ACFM4Z_001381"
                     /pseudo
     CDS             complement(3604..4026)
                     /locus_tag="ACFM4Z_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
     gene            complement(4162..4705)
                     /locus_tag="ACFM4Z_001382"
                     /pseudo
     CDS             complement(4162..4705)
                     /locus_tag="ACFM4Z_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
     gene            complement(4832..5059)
                     /locus_tag="ACFM4Z_001383"
     CDS             complement(4832..5059)
                     /locus_tag="ACFM4Z_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021409.1"
                     /translation="MDIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            complement(5118..5441)
                     /locus_tag="ACFM4Z_001384"
     CDS             complement(5118..5441)
                     /locus_tag="ACFM4Z_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFC6021410.1"
                     /translation="MIIDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     QLISYRQDFQISIEQNEFGEMWTCYCSVNIVLSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            complement(5431..5784)
                     /locus_tag="ACFM4Z_001385"
     CDS             complement(5431..5784)
                     /locus_tag="ACFM4Z_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021411.1"
                     /translation="MGIKNEQIIICKKYNTEIYPISDVSKIGVAENVKQANLYPINGL
                     RHRPKGDTNGWYIWAGENFSYDKNFFLPLHTFHLQIWRPEIIPFLTLPPGYRFLIGEN
                     GYEDVWFDELLLNDN"
     gene            complement(5768..6358)
                     /locus_tag="ACFM4Z_001386"
     CDS             complement(5768..6358)
                     /locus_tag="ACFM4Z_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HFC6021412.1"
                     /translation="MVKTADGYKAIARIRVGDRVFAKDETSGVTGYKPVTARYGNPYQ
                     ETVYIKVSDGIGKTQTVRNIIVKPTPLKAYNLTVADWHTYFVKGNQAETEGVWVHNDC
                     PPYKRPNNATTKAQRESVQGKPCVEYGKLAEKMIADHKKPLVVEYYETGTIDKSKMRA
                     IESVQPQCPTCSAKQGGRLSQYSKEQKRKLSNGNKK"
     gene            complement(6494..7039)
                     /locus_tag="ACFM4Z_001387"
     CDS             complement(6494..7039)
                     /locus_tag="ACFM4Z_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC6021413.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYTGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRKPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNTVFPLLVPLYKQEAELVEKKGWNAFE
                     QFLLDNEVDNLSDMNRKPFVW"
     gene            complement(7036..>8322)
                     /gene="mafB"
                     /locus_tag="ACFM4Z_001388"
     CDS             complement(7036..>8322)
                     /gene="mafB"
                     /locus_tag="ACFM4Z_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC6021414.1"
                     /translation="DTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNAKLDRWGNS
                     MEFINGVAAGALNPFISAGEALGIGDILYGTRYAIDKAAMRNIAPLPAEGKFAAIGGL
                     GSVAGFEKNTREAVDRWIQENPNAAETVEAVFNVAAAAKVAKLAKAAKPGKAAVSGDF
                     SKSYTCSFHGSTLVKTTDGYKAIAHIQAGDRVFAKDETSGKTGYKPVTARYGNPYQET
                     VYIEVSDGLGKIQTLVSNRIHPFYSDGKWIKAEDLKAGNRLLSESGKTQTVRNIVVKP
                     TPLKAYNLTVADWHTYFVKGDKAETEGVWVHNDCPYGSSGAKGKSGSERNTAKRGTFR
                     QETLKNAWDLAADGSQPNSKKCPTCGKDVYGNPHNKEKRNTKDGWDGSHNPSWSNRQH
                     SSDRKEQLNDYNKGVSLECRHCNRSGGNKDERFKKK"
BASE COUNT         2209 a         1872 c         1578 g         2663 t
ORIGIN      
        1 acccgtttaa tgtgtatttc cgttttttgg attgtggttt tcaatttgta gcgaatcaga
       61 ttcggtatat acggcattgc aaaaagcgtt tgactctcca atgccgtctg aaaaccggtt
      121 tcagacggca tttgcgttca gtaagaaagg ccgcgcttgt cgcccgaacg tctcgccgca
      181 gcctctgcat aacggctcac ctctttttcc aaattttcca agttcaaagg aaaatcaggc
      241 agtttgtctc cctgtttctc ttcgcggata atccgcccgc catccaaata ccacgtctgt
      301 tgcgcatgat aggtctgtat atccgccgtt acgccatccg ctttcaatgc taccgtcgaa
      361 gattgtgcaa taaaaagatt tccgtttttc aaataatatt cgaaactctg gcgttttttc
      421 ccattgtcga aactccaata gactttttgc ggcagaccgt ccgcatcata gccgaccaca
      481 agactgttcg ccttcatccc ccgtggcatc aattcccgca tattctgata aaacacagaa
      541 tcgcgcgagt ccgacgcaat ccggttgctc tcttcgcgga agtcccaaac cttctgctcg
      601 tcattcgcga catcccggta tttcgccaaa tatacctggg ccatctgata acacccgagg
      661 caatgctcat aaacatcttc cccgattttc ccgcgccccg ccgcatcaaa taccgaaccg
      721 tccggttgcc aaacaacccg atattctcct gtcgtttcat aattttcccc gtgagccgtt
      781 ccgccgtaca catttacaga aaacggacga tcgttccgat acagatattc ggcattaaca
      841 aacgcttccg gcgagcgttg cgaaagcgaa accgcaacca aaccgccctc gccgatatgg
      901 taatccagcc aaacctcttt cccatgttcc tgctccgtta cgtgaaacca tttcgccttt
      961 tctttcaaac gactgagccg gatagcgagc gcgagataat ccttctccga ctgcaacgga
     1021 ccgtcatcca cagctccggc aagactttcc tccgtcctca tcgattcctt cacgatgaca
     1081 accgccctgt cggcatttcg gaacaggcgg gcaagtttcg ccacaaaagc attcggattt
     1141 tcaggtattt cagttgctgt atcgctcaaa aaccaacgcg gattaatctc ataggcaata
     1201 cccgttccca gccaaaaggc aaatacaagt gcaaaaaatg acaacagtac cggtttgaat
     1261 tttttaaaca tatttatttt tcgtttaaca gaatatatcg attatatcag acgagctttg
     1321 attgtttgat ttttaattta atctacggtt gctgccgtac tttaaaaacc cttatgcagg
     1381 ctgcggcttg ttactcttgt ttgcttcggt tcaatttttt aactaattca aatgcttcag
     1441 atcgatattg cctttgccat tttttccaat ctaacggttt tcccgagcgt tcccaatctt
     1501 ctaaaaccga cattttgaaa atttccattt cccaccaata ttcggcatat aaacgtgatt
     1561 gattgggaat gtcggcttct gcccaatcgg caaaatgcaa aaaatgagaa atatcgcaaa
     1621 ctatattatt ttcaggaact tcttcggcaa atagatgctt gactgactgt aacgcttcgg
     1681 tctgatattt ttctgtccat ttctcccatt tttcaggctt tcccgatatt tcccaatctt
     1741 ccaatgcgga agcattcaag atttccaatt cccgccagca atcgtaataa cgagaggatt
     1801 ggttaggaat tttattttcc gcttcggaaa caaattgggt aaaactaaaa atatccttaa
     1861 cgatttttgc aaatctattt ttcataggtt gtcgccccct ccaaattgcg tgccaacaac
     1921 ttcggcaaaa tcaagccggc aagcccctgc atcctgtgcc aatcggagaa aatgccgtct
     1981 gaaagctgaa taccgcttca gacggcattt gccagatttg accttgccgg tgcttacgga
     2041 tgctttttaa atataaagaa ggaaatatcg tctaacgcat cttcggcaaa accttgaaaa
     2101 tccggattgc cttgttttga gcgcaataaa gcaatcactt tatcgcaatc gccgatattt
     2161 tcatgcatcc tagcgagatg tccgaaacag gttaatgaca atctggcaat attcaagtct
     2221 ttatgcttcg acaactccgt caatttctca gaaatccaag ggatttcatc ctcctctgaa
     2281 ctgaaggtca taccgagcat accgtcaata acactctgtt tattttcaga ataaatcatc
     2341 ttttctactt gttccttatc agccatcatt ttttgtccat ttaattagtc gtttgtatta
     2401 aattgccatt atttattttc caatttactt tataactatc ttcataataa tctaattcaa
     2461 aaaaacctga tatttcaata tccaattcca ttattgtttt aatacatttt tcaaaataaa
     2521 taatgaaata agattttacg catgcaccaa aaaaaatata gctgctccaa ttaaaactat
     2581 ttgtcgggaa aatccacccg cttttatata tttttgcaga ttctttctct tcgatattga
     2641 agggacaatt attccaaaaa ttattgatat tttcttggat gatttttata tcatcgtcag
     2701 agcattcaat ccatcctttt atggaaacat atgatgccat gtttaatctc ctaaacctgt
     2761 tttaacaatg ccgccttttg attcaatata tgacttaact tgtgaatgaa caccgtgcct
     2821 gttccgtaca gcaagcacag gttggattaa gattcgacaa tctgtttgga ttgacagaaa
     2881 ggtcgtctga aaaatcgatt acggtttcag acgacctttt gttgttgagt tttcaaccca
     2941 acctatgcct gccattaatg ttattcatcg tagtaaggtt ctatcgaata attgtctttg
     3001 cccccggcaa tgatagtaac aattttccct tttgcttccc aagcttgtac tcctatttca
     3061 tcaaactcat agacatatgt cggataagat tcattcgata aataacattt atcaatatcg
     3121 tttaacttgg ggtaatggaa aagttgttta aaaatttcaa aatttagacc tattatatta
     3181 acgcccataa aatataactc ctgataacaa aatatcgaaa taattttgtt tttttcgacg
     3241 gaaatgagta aatttgagtc gggagattca taatattctg ttccaaactc atcaggaggt
     3301 tcatacataa aagtttccag tatgtttttg tactgtgtta tatcagcacc aaaacggaat
     3361 attcctacag aagtaaaagg taaaaattcg ggagttttaa cgaccgcgtc gaccatgctc
     3421 ttctcctttt gtttttcgat tggcattttt ggcaatattt ttgatttttt gcttaatctt
     3481 taagcgttca tttttggaca ttccgggaat aattttattt gttaattcag caatttttga
     3541 ttccgctgat atttgacttc gaccgccatc tccatgtttt tcattcttgg agcttcctgt
     3601 tcttttaggc ggacaagaat tatgaaccca caccccttcc gtttccgcct gattgccctt
     3661 gacgaagtag gtatgccaat cggcaacggt cagattgtag gctttgagcg gtgttggttt
     3721 gacaacgatg ttgcggacgg tttgggtttt gccgctttcg gcaaacagcc tgcttcccgc
     3781 tttcaaatct tccgctttaa tccatttgcc gcccgaataa aacggatgga tgcggttgga
     3841 aatcagggtt tggttgttgc cgatgccgtc tgaaatttca atgtaaacgg tttcctggta
     3901 cggattgccg tatcgggcgg taacaggttt gtatccagtt tttccactgg tttcatcttt
     3961 ggcgaagacg cggtcgccgg cttgaatatg ggcaatggct ttgtagccgt ctgccgtttt
     4021 gactaaagca ccgacgtata agggacggag acaggatttt tgaagaactt agttcccaat
     4081 atcctgtttc aaatgaagga aaggtcgcct gaaaattaaa cacggtttca gacggcactt
     4141 tgattaaatt taagaaaatc cttaccaagt aaacggtttc cgattcatat ccgaaaggtt
     4201 gccaacttca ttatccaaca agaactgctc aaaagcattc caacctttct tttccaccaa
     4261 ttcggcttcc tgtttataca aggggacaag caaagggaaa acgatattgt agtgttcgcc
     4321 ataacagacc tgaaaatcat catcgaaata aaatggggcg gaaacataca gtgcatccat
     4381 ttttgttgaa tcaacaatgc ttctgggcaa ataaacaacc tgccccccga agaaggattt
     4441 ttttattctc aatgatggtt tctgccagcc atcttaaaaa tgggaaaatc tgattttcat
     4501 catattttaa agaacataca aaaagtattt caaatctgct tttataattc agatcatgtt
     4561 tgcttaaccc taaagtaata taagttttta aatccctgtt aggggaagag ggaatagctg
     4621 cgatatttaa tttgtactga cttcccgaat cgcttttcga gccatgagaa atagtgccta
     4681 tatatttttc aatatgatta atcatatttt tcttagcttc ccatcactgt atcgaataat
     4741 cataaaccgg attgagacaa aacgccttgt tcccattgat agaaagtccg tctgaaaagt
     4801 aaaagcgttt cagacggact acgtttgccg actatccgac ccactttatt atccaatgag
     4861 attcttgcag gtattctttt aatatttcta agttttcgac ccagtcatcg taactgtcaa
     4921 atgatgagaa tttgtcattt gaagataaca gtatcagaaa tcccccggta ttttcctgat
     4981 catcaataat ttttatgaat ttttcttcag gattgacccc attcaggata acccccaaag
     5041 tattgaattt tatgtccata gacacaccct aatctttaat gggaatatgg ttttttcaac
     5101 ccaacctaca ctttttgtta gttcccatac gaaccaaaac cttcataatt acaatcattt
     5161 ttttgtgcaa taccaaataa cgtagtttca atagctaaaa tgttttcaag ggaaaggacg
     5221 atattgacgg aacaatagca agtccacatt tctccgaatt cattttgctc aatactgatt
     5281 tgaaaatctt gccgataaga aatgagttgg ctttctattg attgcagatt tttttgagaa
     5341 cctcctataa aaaactctat tatcataggt tttgtcaaat caaaccctag ttcttgatat
     5401 tgtttcagta catctgaagt agatttttcg tcaattatca tttaatagca actcatcaaa
     5461 ccatacgtct tcataaccgt tttccccaat taaaaaacgg taaccgggcg gaagtgtcaa
     5521 aaaggggatg atttcgggtc gccatatttg caaatggaaa gtatgcaagg gcaggaaaaa
     5581 atttttatca taggagaagt tttcaccggc ccagatatac caaccgttag tatcaccttt
     5641 gggacggtgc cttaacccgt taatcggata caggtttgcc tgtttgacat tttcagcaac
     5701 accgattttt gaaacatcgg aaataggata tatttctgta ttatattttt tacaaattat
     5761 tatttgttca ttttttattc ccattactca gtttcctttt ctgttctttt gaatattggc
     5821 ttaatctccc cccttgtttt gcagaacaag tcggacattg cggttgtact gattcaattg
     5881 ctctcatctt agatttgtca attgtccctg tttcataata ctcgacaact aatggttttt
     5941 tatggtcggc aatcattttt tcggctaatt taccgtattc aacgcagggt tttccttgta
     6001 cactttctcg ttgtgctttt gtcgttgcgt tatttggtct cttatagggc ggacaatcat
     6061 tatgaaccca cactccttcc gtttccgcct gattgccctt gacaaagtaa gtatgccagt
     6121 cggcaacggt cagattgtag gctttgagcg gtgttggttt gacaatgatg ttgcggacag
     6181 tttgggtttt gccgatgccg tctgaaactt taatgtaaac ggtttcttgg tacggattgc
     6241 cgtatcgggc ggtaacgggt ttgtatcccg ttaccccgct tgtttcgtcc ttggcaaaga
     6301 cgcggtcgcc gactcggata cgggcgatgg ctttgtagcc gtctgccgtt ttgaccaagg
     6361 tgctgccgtg gaaggagcag gtgtaggatt tttgaagaac tcagttctca atattctgtt
     6421 tcaaatgaag gaaaggtcgt ctgaaaaaca agcttaattt cagacgacct ttaatcaaat
     6481 tcaagaaaat cctttaccaa acaaacggtt tcctattcat atccgaaagg ttgtcaactt
     6541 cattatccag caaaaactgc tcaaaagcat tccaaccctt tttttccacc aattccgctt
     6601 cctgtttata caacgggaca agcaaaggga aaacggtatt gtagtgttcg ccataacaga
     6661 cctgaaaatc atcatcgaaa taaaatgggg cggaaacata cagtgcatcc attttgctac
     6721 cttcgataat taccctgggt aattgaataa tttcacctct taaaataggt tttctattgt
     6781 caatcaaata ttcggctact cctgatagaa aagctgcaat attttcttta ttccattctt
     6841 cagaacaggt aaaaattaat tcaaacctac tcttatagtt caagtcatat cgattcaagc
     6901 ctaaagtaga aaaagtagtt acccctgtat atggttgtga tgggaatacg gaaacgctga
     6961 gatgataacg tcgatccaag cattttatac cgctttcgat ttcacccaaa taatgctcta
     7021 aatgttcaac tataatcatt ttttcttaaa cctttcatcc ttgttgccac cggatcgatt
     7081 gcaatgtctg cattctaaac ttactccttt attataatca tttagttgct ccttacgatc
     7141 agaactatgt tgacggttac tccatgatgg attatgactt ccatcccaac catcttttgt
     7201 attacgtttc tccttgttat ggggattacc atatacatct ttaccacatg taggacattt
     7261 ttttgaatta ggctggctac catctgctgc caaatcccat gcatttttta gcgtttcttg
     7321 tctgaaggtt ccacgtttag cagtatttct ctcacttcca cttttacctt tcgcgccaga
     7381 actaccatac ggacaatcat tatgaaccca aaccccttcc gtctccgcct tatcaccctt
     7441 gacgaagtaa gtatgccagt cggcaacggt cagattgtag gctttgagcg gtgttggttt
     7501 gacaacgatg ttgcggacag tttgggtttt gccgctttcg gataacagcc tgtttcccgc
     7561 tttcaaatct tccgctttaa tccattttcc gtccgaataa aatgggtgga tgcggttgga
     7621 aacaagggtt tgtattttac cgaggccgtc tgaaacttca atgtaaacgg tttcctggta
     7681 cggattgccg tatcgggcgg taacaggttt gtatcccgtt tttccactgg tttcatcttt
     7741 ggcgaagacg cggtcgccgg cttgaatatg ggcaatggct ttgtagccgt ctgtcgtttt
     7801 gaccaaggtg ctgccgtgga aggagcaggt gtaggatttt gagaaatccc cgctaaccgc
     7861 agctttcccc ggtttcgccg cctttgccag cttcgcgact ttggctgctg cggcaacgtt
     7921 gaagacggct tcgacggttt cggcggcatt ggggttttcc tgtatccacc ggtcaacggc
     7981 ttcacgcgta ttcttttcaa agcccgccac gctgcccaag ccgccgatgg cggcgaattt
     8041 gccctcggcg ggtaaggggg cgatgttgcg cattgcggct ttgtctatgg catagcgcgt
     8101 tccgtacagt atgtcgccta tgcccaaggc ttcgcccgcg ctgataaagg ggttgagcgc
     8161 gccggcggcg acgccgttga taaactccat gctgttgccc cagcggtcga gcttggcatt
     8221 gtgctcgaac atttttctgt tggcttcatc ggcgcggtcg gagaaattgc tgccgaggtt
     8281 gttgtagttg tcggatatgc gttgccggat gctgcgggtg tc
//
LOCUS       DAXHNE010000084         8248 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.084, whole genome shotgun sequence.
ACCESSION   DAXHNE010000084 DAXHNE010000000
VERSION     DAXHNE010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.084"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(149..1081)
                     /gene="cysK"
                     /locus_tag="ACFM4Z_001389"
     CDS             complement(149..1081)
                     /gene="cysK"
                     /locus_tag="ACFM4Z_001389"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFC6021415.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1275..1880)
                     /locus_tag="ACFM4Z_001390"
     CDS             complement(1275..1880)
                     /locus_tag="ACFM4Z_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021416.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(1877..2728)
                     /gene="dapF"
                     /locus_tag="ACFM4Z_001391"
     CDS             complement(1877..2728)
                     /gene="dapF"
                     /locus_tag="ACFM4Z_001391"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFC6021417.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAEWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQVVGRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVVLPGGTLYIEWACGGDVMMTGPAEAVF
                     EGELAYS"
     gene            2865..3161
                     /locus_tag="ACFM4Z_001392"
     CDS             2865..3161
                     /locus_tag="ACFM4Z_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021418.1"
                     /translation="MFRILLLLFPCAILAACTRNIPANPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            3175..3843
                     /locus_tag="ACFM4Z_001393"
     CDS             3175..3843
                     /locus_tag="ACFM4Z_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6021419.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(3916..6039)
                     /gene="pnp"
                     /locus_tag="ACFM4Z_001394"
     CDS             complement(3916..6039)
                     /gene="pnp"
                     /locus_tag="ACFM4Z_001394"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFC6021420.1"
                     /translation="MMFDKHVKTFQYGNQTVTLETGEIARQAAAAVKVSMGDTVVLVA
                     VTTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRP
                     LFPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNG
                     VYVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAIN
                     AINEFADEVNPELWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWS
                     AVKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTS
                     VLPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEV
                     GRMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCL
                     SLLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQ
                     MDIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKI
                     REVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGK
                     VYEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEV
                     DDRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6273..7136)
                     /locus_tag="ACFM4Z_001395"
     CDS             complement(6273..7136)
                     /locus_tag="ACFM4Z_001395"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFC6021421.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLAHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRAKAVRLYTEAAEYAKSRGIIICDTKFEFGLDENGTLTLMDE
                     VLTPDSSRFWPADQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7336..8199
                     /gene="rfbD"
                     /locus_tag="ACFM4Z_001396"
     CDS             7336..8199
                     /gene="rfbD"
                     /locus_tag="ACFM4Z_001396"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFC6021422.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALSAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLAAAIIRLLQQTSPVRGIYHYAGGKSVSWYE
                     FVQHIFQAASQQQTSFPIPELTAVSDKEYPTAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
BASE COUNT         1983 a         2380 c         1909 g         1976 t
ORIGIN      
        1 atatttccct caagcaatgc ggcggcacca atacctgatt aaaacacaac cggtgttttc
       61 ggcatatatg caaaaacgca ttttccggca cggcgttaca ccgataccga aaaatgcgtc
      121 tgaatgtttc gacaatccga ttaaagcatt atgccaaatc tgcaaaaagt ggcgtagaga
      181 gatagcgttc gccataagaa ggcagcagca cgactatcag cttgccttcg ttttcaggct
      241 gtttggcaag ctgcaacgca ctccaaaccg ccgcaccgga agaaataccc accaaaatgc
      301 cttctttttc cgctattgcg cgggcggttt caaaagccgc ttcgttcgac actttggtaa
      361 tgctgtcgta aattttggta ttcaaaacgg tcggaataaa gcctgcgccg atgccttgaa
      421 tcgggtgcgg gcctttttcg ccgccgctca atacgggtga agcttcaggc tcgacggcaa
      481 ccactttaac ttcgggtttg tattttttca acacttcgcc cacgccggta atcgtaccgc
      541 ccgtgccgac gccggcaacg aagacatcga ctttgccgtc cgtatcccgc caaatttcct
      601 cggcggttgt tttgcggtgg acttcggggt ttgcctcatt gtcgaactgg cgcggcataa
      661 aataagtgtc gggatgcgcg tccaccaagg atttcgcttt ggcaatcgcg cccgccatac
      721 cttcggcggc aggggtcaga atcagctccg caccaaacgc gcgcaacagc attttgcgct
      781 ccttactcat gctttccggc atggtaatcg ccagcttgta gccgcgtgcg gcacatacca
      841 ttgccaaacc gacacccgta ttgccgctgg ttgcttcgac aatgacggtg tttttgttga
      901 ttttgcccgc tttttcggca ccctcgatca ttgcttcggc aatgcggtct ttgacgctgc
      961 tgcctggatt gaaaaattcc agtttcacgg caacctccgc cttcaaacct tcggtcagac
     1021 ggttcagttt gaccaaaggc gtgttgccga ttagttcggt gatgctgttt gcaattttca
     1081 tcttctgctc ctgaataata aggtttcgac agtaaggcgc aatccccata ggcatcctgt
     1141 ccgattgcag gggatttgcg ccgaaacata aacaaatttc ggatatggct ttatcctaaa
     1201 cctatgccgc ctaaaaaacc aatatccaaa atttctttct ttataacttt ttgttccgat
     1261 acggccgcac cgtattatct tccaatttct gaaagcagca gcaccagtac cgagacctgc
     1321 atcaacgctg ccgtcaaccc gccgagtcgc agctgcgagc cgccgaacgt tgacggcggc
     1381 tgccggtcta taccgcagcg gtggacatag gcaaccgcca tcagcacaaa ctgcaacacg
     1441 aaccaataaa ccggcttcag cgcataaagt ttgagcagtg ccgcaaacac aaacacttga
     1501 accgttcccg aaaagcaata atgcaccaac gcacatattc cgacaaaagc aagcgtcatc
     1561 gatacattac tgacggcaaa aagcccgagc agcggatgtt cggggtctgc ctgccatccg
     1621 tttccatctg ttttccggtt ccaatgcccg atgaaaaaaa atatgctgcc caaagtcagg
     1681 taaaaatggg ggatgaacaa gggcgcggcg cgggtcattc cccatatgcc gggcatcagc
     1741 tttgccccca aaaccgatat gcccagccac agcataatac tcgcccaaaa ccattgcaac
     1801 attcctgcgc ggaatatcag aaacagaaaa accgccgcat acagtaaggc agacaaaaaa
     1861 tccaaatgca gcaaaatcat gaatacgcca actcaccttc aaacaccgct tccgcagggc
     1921 cggtcatcat cacatcgccg ccgcaggccc attcgatata taaagtcccg cccggcaaaa
     1981 ccacctctac cgttttccct tcatccaaca gccccagacg gatacccgcc accacagccg
     2041 cacacgcgcc cgtaccgcaa gcttgggttt cgcccacgcc gcgctcgaac acgcgcaaac
     2101 gaatcgcggt tcggccgaca acctgcataa agccgacatt gacgcgttcg ggaaactgcc
     2161 tgtgcggttc gataagcgaa ccggtttcgc gcaccggcgc gcattccacg tcatcgacca
     2221 caatcaccgc atgggggttg cccatattga cgcagctgac aggctgaatg ccggattcga
     2281 gatgcacccc gtaaatacag gcatcatccc cctcgcccga ttcggggaca aacggtattt
     2341 cagacggcat aaacttcggt ttgcccatat tgaccgtaac cataccgtta tcggacaatt
     2401 tcggaaaaat aacgccattt gccgtttcaa cacaaatttc tttcttatcg gtcaaaccct
     2461 tgtctgcaac aaaacgggca aaacaacgcg ctccgttgcc gcattgcccg acctcgctgc
     2521 cgtcggcatt gaaaatacgg taacggaaat ccacgccttc ggtttccgaa cgcccgacca
     2581 ccaaaagctg gtcgaagccc acgccccgga agcggtccgc ccattccgca atcggcgcgt
     2641 cctcgggggt aaaatcctga ctgaccgcgt caatcaccat aaaatcgttg cccaaaccgt
     2701 gcattttggt aaatttcaaa gtgttcatat tgttccgaaa tcccgtatcg tctgttgctt
     2761 ccgaatccga aatcccccgc accgaaggga aatgcttcca tctcccgccg cctgaggatt
     2821 aaaatcagcc attatactgt ttgttacccc ttacaagaaa caccatgttc cgcatattac
     2881 tgttgctttt cccttgtgca atccttgccg cctgcacccg aaacataccg gcaaacccgg
     2941 ccgtttcaaa ttgcgcgcat cttcaaaacc gcaccatcct gtattttgac gcacacggca
     3001 aacccgccga cggcgcagta aagggcggat tttacaaaat tatcgaaata cagggtgcaa
     3061 actgcatcct tattcaagac tttaacagca cgggcaatgc cctcggcgga tcctatgtgc
     3121 cggactgccg caaatcatcc cttttcccga aaaatatttg acttcaaact gcctatgaac
     3181 cacgacatca ctttcctcac cctgttccta ctcggtttct tcggcggaac gcactgcatc
     3241 ggtatgtgcg gcggattaag cagcgcgttt gcgctccaac tccccccgca tatcaaccgc
     3301 ttttggctga tcctgctgct taacacagga cgggtaagca gctatacggc aatcggcctg
     3361 atactcggat taatcggaca ggtcggcgtt tcactcgacc aaacccgcgt cctgcagaat
     3421 attttataca cggccgccaa cctcctgctg ctctttttag gcttatactt gagcggtatt
     3481 tcttccttgg cggcaaaaat cgagaaaatc ggcaaaccga tatggcggaa cctgaacccg
     3541 atactcaacc ggctgttacc cataaaatcc atacccgcct gccttgcggt cggaatatta
     3601 tggggctggc tgccgtgcgg actggtttac agcgcgtcgc tttacgcgct gggaagcggt
     3661 agtgcggcaa cgggcgggtt atatatgctt gcctttgcac tgggtacgct gcccaatctt
     3721 ttagcaatcg gcattttttc cctgcaactg aaaaaaatca tgcaaaaccg atatatccgc
     3781 ctgtgtacgg gattatccgt atcattatgg gcattatgga aacttgccgt cctgtggctg
     3841 taagccccat tcattcccga tacccgtaaa aaataccgtc tgaaacctgt tcagacggca
     3901 ctttcaccct aagcgttact cggcggcatt ttcctcacgg gcaggcgcgt ccagcagggc
     3961 tttgatggac agacggacac ggcctctgtc gtccacttcc aatgctttca cgttcaccac
     4021 ctgaccgact tgcaggtagt cgccgacatt gcgtacgcgc tcgtgggcga tttggctgat
     4081 gtgtaccaaa ccgtctttgc ccggcatcac gctgacaatc gcgccgacat tgttgtcgag
     4141 gattttcacc acagtgcctt cgtacacttt gcccacttcc acttcggcag taatctgctc
     4201 gatgcgtttt ttcgccgcat cgccggcttc ttgagtggtt gcggcaatgg taatcgtacc
     4261 gtcttcggca atattgattt ccgtaccggt ttcagcggta atcgaacgga tggtttcacc
     4321 gcccttaccg ataacttcgc ggattttgtc ttggttgatt ttcatcgtga acaagcgtgg
     4381 cgcgtgtgcg gacagctctt gcgggcccgc aacggcggct ttcatctgat ccaagatgtg
     4441 cagacgcgct tctttggcct gtgccaaagc gatttgcata atttctttgg taatgccttg
     4501 gattttgatg tccatttgca gcgcggtaac gccttcggtc gtaccggcca ctttaaagtc
     4561 catatcgccc aagtggtctt cgtcgcccaa aatgtcggtc aggacggcaa atttgttgcc
     4621 ttccagaatc agacccatcg cgataccggc aacgtgtgct ttcaaaggca cgccggcaga
     4681 cagcaggctc aggcagccgc cgcagacgga agccatagag gaagagccgt tggattcggt
     4741 aatttcggag accacgcgca tggtgtagct gaaatcttca ggtttcggca atacggccaa
     4801 caatgcacgt ttagccaaac ggccgtgacc gatttcacgg cgtttcggtg cgcccatgcg
     4861 gcccacttcg ccggtagagt acggcggaaa gttgtagtgc agcataaagc ggtcggtgta
     4921 ttcgccggac agcgcgtcga tgatttgctc gtcgcgcgaa gtacccaaag ttgcaacggc
     4981 caaagcttgg gtttcgccac gggtaaacaa tgcagaaccg tgcgtgcgcg gcaatacgct
     5041 ggtttggatg ttcagcggac ggacggtgcg ggtgtcgcgg ccgtcgatgc gcggttggcc
     5101 atccaaaatt tggctgcgga cgacatcggc ttccaagtgt ttgaaaatgc ctttgatttc
     5161 gttggctgcc aaagtgtcgg tttcttcggt aatcaaggct tcttttaccg cactccaagc
     5221 ttcgtccaat ttggcagaac gcgcttgttt ttgacggatt ttgaacgctt ctttaatggt
     5281 ttcgccggca atcccgcgga ctttggcaac cagttcctca ttggtttcag gtgctttcca
     5341 atcccaaagt tccggattga cttcgtcggc aaattcattg attgcattga tggcaacctg
     5401 catttgatcg tggccgtaaa ccaccgcgcc cagcatcacg tcttcgggca ggattttggc
     5461 ttcggattcc accatcaaca cggcttttga agtaccggcg accaccaagt ccaattgcga
     5521 tttcgccaat tcggctttag tcggattcaa aacgtacacg ccgtttacat aaccgacgcg
     5581 tgccgcgccg atcgggccgg caaacggtac gccgctcaac accagcgcgg cagatgcacc
     5641 caacattgca ggaatatcag aatcgatttc aggatcgacg gacacgacca tcgctacgat
     5701 ttggatgtcg tggtagaaac cttcagggaa cagcggacga atcggacggt cgatcagacg
     5761 gctggtcagg atttcttttt cgctttgttt gccttcgcgt ttgaagaaac cgccgggaat
     5821 tttgcctgcg gcgtaagtgc gttccaaata atcgacggtc agggggaaga agtcttgacc
     5881 ttctttcact tctttgttgg tggtaacggc aaccaaaaca acggtgtcgc ccatagagac
     5941 tttaacggca gcggcggctt ggcgggcaat ttcgccggtt tccaaagtaa cggtctgatt
     6001 accgtattgg aaggtcttaa cgtgtttgtc gaacatcatt gttcctttca aaataccgca
     6061 ctgctaaaac actaataatg cacactaaaa tccgaatgtg catagttagg gtttcagacc
     6121 gtgcggcagg ttataaacaa gctttcagac ggcatttcgg acgctgaaag caattgcgga
     6181 ttataaaggc aaccatcctt aaaatccagt attgatacaa taaaaaggcc gtctgaacca
     6241 tatttctttc agacggcctt caaacttaaa aatcaatcct gagtcagcaa agtcaatgct
     6301 tcccgatact tctcgacagt tttctgaatc acatcggcag gcactttcgg cgcaggggct
     6361 tttttgttcc aaccgctttg ttccagccag tcgcggacga attgtttgtc aaaagacggc
     6421 ggattggtgc cgactttgta ttggtcggca ggccaaaaac ggctcgaatc gggagtcaat
     6481 acctcatcca tcagcgtcag cgtaccgttt tcatccaaac cgaattcaaa tttggtatcg
     6541 caaataataa taccgcgcga tttggcatat tccgccgctt cggtgtaaag ccgaaccgcc
     6601 ttggcgcgca cttcttccgc caattctttg ccgataatgc gtccgcattc ttcaaagctg
     6661 atgttttcat cgtgatcgcc gactgcggct ttggttgagg gcgtaaaaat cacttcaggc
     6721 agttgttgcg cttcctgcat accttcaggc agttgaatac cgcaaaccga gccggttttt
     6781 tgataatctt tccaaccgct gcctgccaga taaccacgca caatcgcctc tactttcacc
     6841 ggagtgagct ttttagccac gacggcgcgt ttctctaaag ctttggcttc gttttcaggc
     6901 aaaacatcgt aaaccgtttg accggtaaag tggttgggca taatatgcgc cagtttttta
     6961 aaccaaaaat tggaaatctg cgtcagaatc tcccctttgc tcggaatcgg gtcgtccaaa
     7021 atcacatcaa acgcggacag gcggtcggaa gcgaccatca gcatacgttt atcgtcgatt
     7081 tcatataaat cgcgcacttt tccagaatag atctttacca aaccaatctc actcatttcg
     7141 cacccccctg aaaatatctt gaaaataccg acccgacacc cgacaggttt gaatcacaaa
     7201 ccgatattct aaccgaagtc ggcgcaaaac aatacccatg gcacaaaaag ccaacccgtc
     7261 aaccgtcggc aaagttttgg cactataata ccgacagcaa gtcctacaat acactttcac
     7321 caaaggaaat acctcatgag aatcctattg acaggctcga aaagccaact ggcacgctgc
     7381 ctgcgcgacc gtcttccgga agactgggaa accattgcga cggattccgc atccctagac
     7441 attaccgatg ccgatgccgt ctgcaacatg gtcaaaagtt tccaacccga cgccattgtc
     7501 aacacggctg cctatactgc cgtcgacaaa gcggaaggcg atgcggcagc ggcatttgcc
     7561 gtcaatgctt ccgccgttta caaccttgcc ttgtcagcac atcgcgccca tgcccgattc
     7621 atccacatct caaccgacta tatctttgac ggtaaaggga aaagacccta tcaggaaagc
     7681 gactttacca atccttccaa tgtatacgga caatccaaaa ccgcaggcga gctgctcgca
     7741 ctgtctgcca atcccgacag ccttatcctg cggacttctt ggctgtttag cgaatacggg
     7801 gacaactttg tccgcacgat gctgaacctt gcacgggaac gttccccgct gtccgccgtc
     7861 cacaaccaaa tcggctgccc gacctatgcc ggcgatttag ctgccgccat catccgcctt
     7921 ttgcagcaaa cgtcgcccgt gcgcggcatt taccactacg ccggcggcaa atccgtatct
     7981 tggtacgaat ttgtccaaca tattttccaa gcggcatcgc aacagcagac atccttcccc
     8041 attcccgaat tgactgccgt ttcagacaag gaatatccga ccgccgcccc caggcccgca
     8101 tacagcattt tggactgccg caaaatcgaa aacgacttcg gcatcaaacc gtcagactgg
     8161 caaaaagccc ttgcgcaggt cgtttccaag ctgctctgat gccgcccgcc tctccgtttc
     8221 cgccgtcaag caccgccttg gcggtttt
//
LOCUS       DAXHNE010000085         8131 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.085, whole genome shotgun sequence.
ACCESSION   DAXHNE010000085 DAXHNE010000000
VERSION     DAXHNE010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8131
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.085"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(254..1513)
                     /gene="rho"
                     /locus_tag="ACFM4Z_001397"
     CDS             complement(254..1513)
                     /gene="rho"
                     /locus_tag="ACFM4Z_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFC6021423.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTSNYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            complement(1733..4117)
                     /gene="ppsA"
                     /locus_tag="ACFM4Z_001398"
     CDS             complement(1733..4117)
                     /gene="ppsA"
                     /locus_tag="ACFM4Z_001398"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFC6021424.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGNRNLRRFAINGDKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4529..5350
                     /locus_tag="ACFM4Z_001399"
     CDS             4529..5350
                     /locus_tag="ACFM4Z_001399"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFC6021425.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASGQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(5414..6076)
                     /locus_tag="ACFM4Z_001400"
     CDS             complement(5414..6076)
                     /locus_tag="ACFM4Z_001400"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6021426.1"
                     /translation="MIQAVLFDLDGTLADTALDLGGALNTVLRRHNLPEKSIAEIRPY
                     AGHGAAGLLKLGAGITPDHPDYARWRTEYLDEYDRRYAQDTALFDGVDELIAELGRRG
                     IKWGIITNKPMRFTDKLVPKLGFAIPPAVVVSGDTFGEPKPSIKPMLYACRQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYIAPEDDTGSWQPDYRIATPIELIGCLNKK
                     QV"
     gene            complement(6130..7089)
                     /locus_tag="ACFM4Z_001401"
     CDS             complement(6130..7089)
                     /locus_tag="ACFM4Z_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFC6021427.1"
                     /translation="MSHHHEHHHHAHAHTHTANKKVLRVSFIIIAAFMLLEAFGGWLT
                     HSLALLSDAGHMFSDAFSLGVALWAFKLGEKETTLQKTFGYKRFEILTAMFNGLSLVV
                     IAVLIFYEAVKRLLYPPEIATLGMLVISVVGLLVNIGVAVYMLKNSDTEANVNMRGAY
                     LHVLSDLLGSIGAIAAAVLMMAFGWKWADPLASVFVAALVGRSGWSLLKQTLHILMEG
                     APENIHTDDLLAIIRNTDGVKSVHDLHVWTITSNINVLSCHIVVDGSMTVAESEQIAY
                     RIEHELSHKNIGHCTIQIESEHHPHKNEILCSPYAEAHHSHSH"
     gene            complement(7135..7962)
                     /locus_tag="ACFM4Z_001402"
     CDS             complement(7135..7962)
                     /locus_tag="ACFM4Z_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFC6021428.1"
                     /translation="MKLDIYALLERALLSGDPDEKGRLTDEAFAAVQNADGAEANAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTTEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRERLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYSDEVGHVAIGNHWYQHLCRE
                     RGLEPIALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQSLKQGKKVV"
BASE COUNT         1660 a         1977 c         2444 g         2050 t
ORIGIN      
        1 aatttataca aaaatgagcg cggttcggcg caaccttgaa tcaagttccc gcatcggttt
       61 tcattgccgg tacggatgcg ttcaagccgg ttttgcaaag gccgcgcttt cggcaagcgg
      121 acacggacac tgccgacggt tgcgccgtta acggggggag ggaggagctg cgccgaccgt
      181 gtgaatgaaa gtgccgtctg aaacccgatt ttcaggcttc agacggcatt tcgcattaat
      241 gcgggcggcg cgtttatttg ccgcgcatca gttcaaagaa atcgtcgttg tttttagagg
      301 ctttgatttt cccgtttaag aactcggtag cttcgatttc atccatcggg tgcaggaact
      361 tgcgtaagag ccacatacgt tgcaactggt cgttcggcac aagcagctct tcgcggcgcg
      421 tgccggattt gttgatgttg atggcgggga agaggcgttt ttccgccata cggcggtcga
      481 ggtgcaattc catattgccg gtgcctttga attcttcgta aatcacatcg tccatacggc
      541 tgccggtttc aaccaatgcg gtggcaatga tggtcagcga accgccttct tccacgttgc
      601 gcgccgcgcc gaagaaacgt ttggggcggt gcagcgcgtt ggcatcgaca ccgccggtca
      661 ggattttgcc cgaggtaggc acgacggtat tgtaggcgcg ggcaaggcgg gtaatcgaat
      721 ccagcaggat gaccacgtct tttttgtgtt ctaccatacg cttggctttt tcaagcacca
      781 tttcggcaac ttggacgtgg cgttgcgccg gttcgtcaaa ggtggaggaa accacttcgc
      841 cgcggacgga acggctcatt tcggttactt cttcgggacg ctcgtcaatc aagaggacga
      901 tgagttcgac ttcgggatag tttgaggtaa cggcgtgggc aatgttttgc agcatcacgg
      961 ttttaccgct tttgggcggg gcaaccaaga gggcgcgctg acctttgccg ataggggaaa
     1021 tcaggtcgat ggcacgtccg gtcaggtttt cttcggactt taagtcgcgt tccagcttca
     1081 actgttcggt cggaaacagc ggggtcaggt tttcaaacag gattttatgg cggcatactt
     1141 ccgggtggtc gccgttgatg gtatcaagcc tgaccagggc aaaatagcgt tcgttgtctt
     1201 ttgggacgcg cacgcttcct tcgatggtgt cgcccgtatg caggttgaag cggcggattt
     1261 gggtgggcga gacatagatg tcgtcggggc cggcaagata ggacgtgtcc gcgctgcgga
     1321 ggaagccgaa gccgtcgggc aggatttcaa gcgtgccgga gcaggtgaaa ccctcgcctt
     1381 ttttcatcat ctggcggacg atggcaaata cgaggtcttg tttgcggaat cggttggcgt
     1441 tttcgatgcc gtgttcttcc gccaattcta agagtttgga aatgtgcagg gtttgtaatt
     1501 cggagacgtg cataataatg atgtattttg aagaggaaaa agacaggcag atgccgtctg
     1561 aaagaagaag ctgaccgttg ccggttgctc ggggaagggg gaattgtagg cagtcggcgc
     1621 gtgggtgtca aatattatcg cggacggggc atcggcagaa aatgccgtct gagcggagct
     1681 gcttggaaaa aaataccccc gcgcttttca ggctcggggg tatgggcatt gattatttgt
     1741 tcaattcatt cgccaaatac agccaagttt cgatgacggt atccgggttc agagaaacgc
     1801 tttcaatgcc ttcctcaacc agccatttgg cgaagtccgg atggtcggac gggccttgac
     1861 cgcagatgcc gacatatttg ttctgcttgc ggcaggcgga gatggcaagg tgcagcatca
     1921 ctttgacggc agggttgcgt tcgtcaaacg attcggatac caagccgctg tcgcggtcga
     1981 gaccgagggt cagttgggtc atgtcgttcg agccgatgga gaagccgtcg aagtattgca
     2041 ggaattgttc cgccaatacc gcgttgctcg gcagctcgca catcataatc aggcgcaggc
     2101 cgtttttgcc gcgttccaag ccgttttctt tcagggcttt gacaacggct tcggcttcgc
     2161 ccaaagtgcg gacgaacgga atcatgattt caacgttggt caaccccatt tcatcgcgga
     2221 cgcgtttcaa ggctttgcat tccaaggcga aacagtcttt gaagttgtcg gcgacataac
     2281 gcgccgcacc acggaagccc aacatcgggt tttcttcatg cggttcgtat acgttgccgc
     2341 cgaccaggtt ggcgtattcg ttggatttga agtcggacat acggacgatg gttttacgcg
     2401 gataaaccga tgcggccaat gtcgccacgc cttcggcgat tttatcgacg tagaagtcga
     2461 caggggacgc gtaaccggcg atacggcggg taatttccgc ttttaattcg tcgtcttgtt
     2521 tgtcaaattc caacaaggct ttggggtgga taccgatttg gcggttgatg ataaattcca
     2581 tacgcgccaa gccgatgcct tcgctgggca ggttggcgaa gctgaatgcg agttcggggt
     2641 tgccgacgtt catcatgact ttgacgggtg ctttgggcat attgtccaag gcgacatcgg
     2701 taatctgtac gtccaacaga ccggcataga tgaagccggt atcgccttca gcacaggata
     2761 cggtaacttc ttgaccgttt ttcagcaatt cggttgcatt gccgcagccg acaacggcag
     2821 gaatgcccag ttcgcgcgcg ataatcgccg cgtggcaggt gcgtccgcca cggttggtca
     2881 cgatggcgga agcccgtttc atcacgggtt cccaatccgg atcggtcatg tcggtaacga
     2941 gtacgtcgcc ggcttcgacg gaatccatct cggaagcatc tttaatcagg cgcaccttgc
     3001 cctgaccgac tttctgaccg atggcgcggc cttcgcataa tacggttttg tcgccgttga
     3061 tggcgaagcg gcgcaggttg cggttgccct cttcttggga ttttacggtt tcgggacggg
     3121 cttgcaggat gtagagtttg ccgtccaagc cgtcgcgtcc ccattcgata tccatcgggc
     3181 ggccgtagtg tttttcgatg gtcagtgcgt aatgcgccaa ctcggtgatt tcttcgtcgg
     3241 taatggagaa gcggttgcgg tcttcttcgg ggacatcgac gttggttacc gatttgccgg
     3301 cttcggcttt gtcggtaaaa atcattttga tgtgtttcga acccatggtt ttacgcagga
     3361 tggcgggctt gcccgctttg agcgtgggtt tgaacacata aaattcgtcc gggttgaccg
     3421 caccttgtac gacgttttcg cccagaccgt aagaggaggt aacaaagacg acttgatcgt
     3481 agccggattc ggtgtcgagg gtgaacatca cacctgatgc gccgctgtcg gaacgcacca
     3541 tgcgttgaac gccggcggaa agggcgacga tgtcgtgttc gaagcctttg tggacgcggt
     3601 aggaaatggc gcggtcgttg tacagggagg cgaatacatg gtgcatcgct tctttaacgt
     3661 tttccaatcc gttgatgttc aagaaggttt cctgttggcc ggcaaatgat gcgtcgggca
     3721 ggtcttcggc ggttgcggaa gagcgtacgg caacggaaat gtccgcaccg ccggcatcgg
     3781 caaccatttt gttccatgcc gcttcgattt cggcatcgag ctgttcgggg aagggcgtgt
     3841 ccaaaatcca ttggcggatt tctttgccga cgcgtgccag ttcggcaacg tcttcgacat
     3901 ctaattttgc cagtgcggcg gaaatgcgtt cgctcagacc gttgtgtgcg aggaatgcgc
     3961 ggtaggcatc ggctgtggtg gcaaagccgc cggggacgcg aacgcctttt tcggtcagct
     4021 gactgatcat ttcgcccagc gaggcgtttt taccgcccac gcgttcaaca tctgtcatac
     4081 gcaggttttc aaaccagatt acgtagttgt cggccatttg tgtgtccaat ccaaaatatg
     4141 ttaaaaaaga aacaaatccg cgtgcttatt ttaagcgatt cgttcctctg ctgtcatgtg
     4201 ttttatccgt tttaaaatca tgatgccgtc tgaaaaattg cggtttcggc gtgtgtagcg
     4261 gtttgaaact tacagccggt atacttcttt ttttgggtat tttctttgta aaacaggtgg
     4321 tttgaatagg ttaatgtttt ttctgtttga tttttttgtt tattttttaa aattttctgc
     4381 caaaaaatac tttatataaa taattttatt tcaaaattat attgtgtctg tttgggtgta
     4441 atccgaggta ggtgtgctgc gagtgctttc cttgtgtctg ctgctgctgt tatgatggga
     4501 ttttaaacct gtgttttaag gatggaagat gagcagtccg cgccatgtgt tttacatttc
     4561 cgaccgtacc ggtctgactg ccgagaatat cggcgaggcg ttgctgaacc agtttggcaa
     4621 tctgtcgttc aaacgccata cgcatccgtt tgtcgatacg ccggaaaagg cgcgcgcggt
     4681 ggtggagaag gtcaatcgga gccggcagga aaacggtcag cgtccgattg cgtttgtcag
     4741 tgtggttgat gacgaaatcc gtcggattat caaaggggcg gatgcttttc agattaattt
     4801 ctttgagact tttttgggac tgttggagaa agaactcaat accgaagcca cggcatccgg
     4861 gcaggggcat cacagtatcg gtaatacgaa gcgttacgat gcgcgtatgg aggcggtgaa
     4921 tttctcattg aaccacgatg acggggtcag cgataagaac cttcaggaag cggatgtaat
     4981 cttgatgggt gtatcgcgtt cgggcaaaac gccgacctgc ctttacctcg ccctgcaata
     5041 cggcatccgt gcggcaaact atccgctgat tcccgacgat ttggaatcgg ccgatctgcc
     5101 gcgtatggtc aagccttata gggataagct gttcgggttg accatccagc cggaacgttt
     5161 gcaggccatc cgccaagagc gccgcccgaa ttcaacttat gccaaaatcg atacatgccg
     5221 cagcgaggtg gcggacgcgc agagtatgtt cagacggcat gggattccgt ttgcgaatac
     5281 gacggataaa tcggtggaag agcttgccgt acacatcctt caggcgtgca agttgaagcg
     5341 tagattctga tgtggtgtcg ggattgtacc ggagtgtggg aaatgccgtc tgaaaggttt
     5401 tcagacggca tttttatacc tgttttttgt taaggcagcc gattagttcg atcggtgtgg
     5461 cgatgcggta gtcgggttgc catgagccgg tatcgtcttc gggagcgatg tagccccatt
     5521 cggcgaggac ggttttcatg ccggcgttgc ggcctgcttg gatgtcgcgt tcggcatcac
     5581 cgacgtagag tgtgtgttgc gggtcggcgt ggatttgtct gcacgcatac agcatgggtt
     5641 tgatgctggg cttgggttcg ccgaaggtgt cgccgctgac gacgacggcg ggcgggatgg
     5701 cgaagccgag tttggggacg agtttgtcgg tgaagcgcat gggtttgttg gtgatgatgc
     5761 cccatttgat gccgcgcctg ccgagttcgg cgatgagttc gtccacgccg tcgaagaggg
     5821 cggtgtcttg ggcgtagcgg cggtcgtatt cgtcgaggta ttcggtgcgc caccgggcgt
     5881 agtcggggtg gtcgggcgtg atgcctgcgc cgagtttgag cagtcctgcc gcaccgtgtc
     5941 cggcatacgg gcggatttcc gcgatgcttt tttcgggcag gttatggcgg cgcaatacgg
     6001 tgttgagtgc gccgccgagg tctagggcgg tgtcggcgag cgtgccgtcg aggtcgaaca
     6061 atacggcttg tatcatgtgt tttcctttgg tggaggccgt ctgaaaggtt ttcagacggc
     6121 cttgttggtt taatggctgt ggctatgatg tgcttctgcg tagggcgaac acaaaatctc
     6181 gtttttgtgc gggtggtgtt cgctttcgat ttggatggtg cagtgtccga tgtttttgtg
     6241 ggacaactcg tgttcgatgc ggtaggcgat ttgctcggat tcggcaacgg tcatgctgcc
     6301 gtctacgacg atgtggcagg ataatacgtt gatattactg gtaatcgtcc agacgtgtaa
     6361 gtcgtggacg gatttgacgc cgtcggtgtt gcggatgatg gcgagcaggt cgtcggtgtg
     6421 gatgttttcg ggcgcgcctt ccatgaggat gtgcagcgtt tgcttgagca ggctccagcc
     6481 gctgcgtccg accaacgcgg ccacgaacac gctggcgagc ggatctgccc atttccagcc
     6541 gaacgccatc atgagcacgg cggcggcaat cgcgccgatt gagccgagaa ggtcgctcaa
     6601 aacgtgcaga tacgcgccgc gcatgttgac gttggcttcg gtgtcgctgt ttttcagcat
     6661 gtaaaccgcc acgccgatgt tgaccagcag gccgacgacg ctgatgacga gcatccccag
     6721 cgtggcgatt tcgggcggat aaagcaggcg tttgacggct tcgtagaaaa tcagcacggc
     6781 gatgaccacc agcgacaaac cgttgaacat ggcggtgagg atttcgaacc gtttgtagcc
     6841 gaaggtcttt tgcagtgtgg tttctttttc gccgagtttg aacgcccaca acgccacgcc
     6901 caaggaaaat gcgtcgctga acatatgccc tgcgtcggaa agcagcgcga gcgaatgggt
     6961 cagccagccg ccgaacgcct ccagcagcat aaaggcggcg atgatgatga acgacacgcg
     7021 caatactttt ttgttggcgg tatgcgtgtg ggcgtgtgcg tgatgatggt gttcgtgatg
     7081 gtgagacata acgttcctca acagaggccg tctgaaaatc cggattcagg gttttcagac
     7141 gacctttttg ccttgtttca aactctgctc gaaatcttcc agcatatcca attcaaagcg
     7201 gctgaagccc gctttttcgc gcgcttcgat gttcacatag ccgcggaaga taaacatatc
     7261 gtaacgggca atcagactgc ggaacagggc aatcggttcc aaaccgcgtt cgcggcaaag
     7321 gtgttgatac caatggttgc cgatggcgac gtgtcccact tcgtcgctgt aaatgatgtc
     7381 caacacgccg caggtttccg aatcgccgcg ctgcgccacc ttcgcgcgta tccccggcgt
     7441 aacgtccagc ccgcgcgctt ccaacacgcg cggtaccaac gccatacgca acagcgggtc
     7501 gtaggcggtt ttgtacgcca tatcccacaa atggttgtgc gcctcgaaac tgccgtaatc
     7561 gaagccgaaa gcgcgcagcc tttcgcgcat caggcggaaa tggtacacct cttccttcgc
     7621 cactttcacc cagtcgcgga caaactgaaa cggcagcgtg cggaaacggt atgccgcgtc
     7681 caaagctaga ttgacggcgt tgaattcgat atgcgcaatc gcgtgcagca tcgccgcata
     7741 accttcggtt gtgttcattt tgcgcggggt cagttgcgac ggcgcgacca aaacaggctt
     7801 gtccggccgt cccgctcggg ggaagtccgc cggcggcgcg tttgcttccg ccccgtccgc
     7861 attttgaacg gcggcaaacg cctcatccgt cagccgtcct ttttcatctg ggtcgcccga
     7921 aagcagggcg cgttccagca aagcataaat atcgagtttc atctcaagtc cgccgtgttc
     7981 ggaaaacaaa tattatagcg ttttaaaaaa acaagatgaa gcatataatc tccgcgattc
     8041 ggcattccgc gcccaaaccg tcaaatatag tggattaaca aaaaccggta cggcgttgcc
     8101 tcgccttagc tcaaagagaa cgattctcta a
//
LOCUS       DAXHNE010000086         8071 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.086, whole genome shotgun sequence.
ACCESSION   DAXHNE010000086 DAXHNE010000000
VERSION     DAXHNE010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8071
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.086"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            94..402
                     /locus_tag="ACFM4Z_001403"
     CDS             94..402
                     /locus_tag="ACFM4Z_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021429.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQIRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            484..1515
                     /gene="ruvB"
                     /locus_tag="ACFM4Z_001404"
     CDS             484..1515
                     /gene="ruvB"
                     /locus_tag="ACFM4Z_001404"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFC6021430.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLIGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(1538..2266)
                     /gene="rpe"
                     /locus_tag="ACFM4Z_001405"
     CDS             complement(1538..2266)
                     /gene="rpe"
                     /locus_tag="ACFM4Z_001405"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFC6021431.1"
                     /translation="MTAYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRRV
                     RAMLDRYEAQSGRHIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            2412..2846
                     /locus_tag="ACFM4Z_001406"
     CDS             2412..2846
                     /locus_tag="ACFM4Z_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HFC6021432.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPLNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(2859..3722)
                     /locus_tag="ACFM4Z_001407"
     CDS             complement(2859..3722)
                     /locus_tag="ACFM4Z_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6021433.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FWEHLPDPHTFPLARLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYLDENAGTTGVRGEIHQKEAVEEKVSLSDRF
                     GLWLSFYPFDQNDYLSAVQSWLEDFDVPYDEAARTAALQWAQMRGNRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(3730..4347)
                     /locus_tag="ACFM4Z_001408"
     CDS             complement(3730..4347)
                     /locus_tag="ACFM4Z_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFC6021434.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGYNRTVWFALPHELKPYILTKGFVGLDGCSLTIGKVEDSRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(4510..5124)
                     /locus_tag="ACFM4Z_001409"
     CDS             complement(4510..5124)
                     /locus_tag="ACFM4Z_001409"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HFC6021435.1"
                     /translation="MKTSNFSKRTSAMKTFALILADGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPDDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARSVRFEFDGHFADLNTQTDLQEG"
     gene            5330..7102
                     /locus_tag="ACFM4Z_001410"
     CDS             5330..7102
                     /locus_tag="ACFM4Z_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HFC6021436.1"
                     /translation="MILPARFSDGISLSLRLKLLTGLWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRAQIDNQVAEFEKSLKRIAQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGTKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIRAV
                     LKIYSQPQQGTTVSLAGVSEESLK"
     gene            7099..7755
                     /locus_tag="ACFM4Z_001411"
     CDS             7099..7755
                     /locus_tag="ACFM4Z_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6021437.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECIRIGARGY
                     LLKNINADFLLESIRKVAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVLE
                     "
BASE COUNT         1976 a         2372 c         1892 g         1831 t
ORIGIN      
        1 ttttgaggac gatacccaat ccaagctttg caacagccgc cgccatatcc gctataattc
       61 acgcttcagc cattccgccc ccgacataaa atcatgaccc tgaaaaccga tttattgcct
      121 aaaatcaaca acgaagatta tcaacgcctc atcctcaaac acagtgcgga atttagcggt
      181 ggcgaaatcc gcctgttgaa cgaaatcctc gaaaaattca atttcgacgt tgttcaggcg
      241 caggcattgg cgcaagccgt catgcagcaa atccgcttcg accccaacgc ctaccacatc
      301 gacagcgacg acgaagacac caccggcatc tgcccccact gcatcaaccc gcctatgccg
      361 cccctgcgcg actatctcgt ttggcgcgaa acccgcggat aaaacgcttt tgaccgttat
      421 cttttcaatg ccgtctgaaa cgccgccgac cgttcggacg gcatacccga caaagggaac
      481 actatgctgc aaaccgacaa cctgaccgcc gcgcaaccgc aacgcatcgt tgccgcccaa
      541 accgcctccg cacaggaaga actgctcgaa cgcgccctcc gccccaaaac gctggacgac
      601 tacatcgggc aagacaaagc caaagaacag cttgccattt tcatccaagc cgccaaaaaa
      661 cgcggcgaag cactcgacca cgttttgctc ttcggcccgc ccggactggg caaaaccaca
      721 ctggcgcaca tcatcgccaa agaattgggc gtaaatttgc gccaaaccag cggccccgtc
      781 ctcgaacgcg caggcgacct cgccgccctt ttgaccaacc ttgatccgca cgatgtattg
      841 ttcatcgacg aaatccaccg cctcagccct gttgtcgaag aaatcctcta tcccgcgctc
      901 gaagactacc ggctcgacat tatgataggc gaaggacccg ccgcccgttc cgtcaaaatc
      961 gacctgccgc ccttcacgct catcggcgcg accacccgcg ccggtatgct gaccaatccg
     1021 ttgcgcgacc gcttcggcat cgtctcccgc cttgagtttt acgaaaaccg agaccttacc
     1081 accatcgtca gccgttcggc acaactgttg cagctcgata tgtccgaaga aggcgcggaa
     1141 gaaatcgcca aacgcagccg cggtacgccg cgcatcgcca accgcctgtt gcgacgcgtg
     1201 cgcgatttcg ccgacgtgaa aaacaacggc acaatcgacg gcggcatcgc cgatgccgct
     1261 ttaagtatgc tggacgtgga cgcgcagggg ctggacgtga tggacaggaa atttctcgaa
     1321 gccgttttgc acaaattcgg cggcggcccg gtcggtttgg acaatgttgc cgccgccatc
     1381 ggcgaatcta cagacaccat cgaagacgtt atcgaaccct accttatcca acaaggcttc
     1441 ctgcaacgca ccccgcgcgg caggatggcg accgaacgcg cctacctgca tttcgggctg
     1501 cccgtcgaaa aataacgcaa tgccgtctga aacagagcta attttcagac ggcatttcta
     1561 tttcaatcat tggcgcaagg ttcagcctgc cgcttttttc cagttccgcc ctcatcgcat
     1621 caatcaccgc cttatagtct ggtttgccga aaatcgcaga accggcaaca aaggtatccg
     1681 caccagctcg ggcaacggcg gcaatattgt cggttttgat gccgccgtcc acttcgatgg
     1741 cgatgtgccg cccgctttgt gcttcgtacc gatccagcat cgcccgcacc cggcggattt
     1801 tttcaagggt gtgcgggatg aagctttgtc cgccgaatcc ggggttgacc gacatcagca
     1861 aaaccatatc cagcctgtcc aatacgtttt ccaacagata tacgggcgtt gccggattca
     1921 acaccagccc cgcctgacag cccatatcac gaatcaggct caagctgcgg tcgatatggc
     1981 ggctcgcctc gggatggaac gtgatgattg atgctcctgc tttggcaaac gactgaatca
     2041 ggtcgtcaac gggttcgacc atcagatgca catcaatcgg cacgcttgca taaggcttca
     2101 acgccgcgca aaccataggg ccgaaggtca ggttcggcac ataatggttg tccatcacgt
     2161 caaaatggat cagatctgca cctgccgcaa tgacgctttc cacctcttct ccaaggcggg
     2221 caaagtctgc cgacaaaata ctgggtgcga tacggtaggc ggtcatattt tttccttcaa
     2281 tgcctttacg ggcggactca acgcgggaaa gccccgaaac agcattatta acaaaagtta
     2341 actgccataa taccatcttt cacacggcaa tcaagtatat tgtccgaaat atctggatta
     2401 aaggaaaagc cgtgccactg cccgctccct gccgtttcgc caaacctgcc gcctcttttt
     2461 taagtatggc tttgctttcc tgccagcttt cccacgccgc cacggcttat atccccctga
     2521 acgattttca accgaactgc gacatacgcc gactcggact gacacagggt cagcacaatg
     2581 aactgcgtaa aatccgcgcc gccttcaaaa tggcgggcga cagggcgcgt ttgaaggtta
     2641 tgcattccga acacagccgc cgccggtctg tcgtcgaaat catttcttcg gatgttttta
     2701 atcggaacga ggcgcgcgat tatgtcgaaa gccgctattt gtccggtatg gattttgcgg
     2761 tggacgaatt ggaaatccaa caccggttct tccatatcct cacaccgcaa cagcagcaaa
     2821 tgtggctttc ttcctgcctc aaataatccc cgaaacgctc acaacgcccg ttgtttcggc
     2881 agcctgcccg cccagtcgca ggcaaactgc cacgcggaac gtcccgagcg gttgccccgc
     2941 atctgcgccc actgcaacgc cgccgtccga gctgcttcgt catatggcac atcaaaatct
     3001 tccaaccagc tttgcactgc cgacagataa tcgttttgat cgaacggata aaagctgagc
     3061 cacaatccga atcggtcgga caaagatact ttttcttcca ccgcttcttt ctgatggatt
     3121 tctccgcgca cgcctgtcgt acctgcattt tcatccaaat attcgggcat caggtgccgt
     3181 ctgttggaag tcgcgtacac cagcacgttg gcgcaacgtt gggacaaacc gccgtccaat
     3241 gccgttttca atgccttata ggtttcgtcg ccgctttcaa atgacaaatc gtcgcaaaac
     3301 acaatgaatt tttccggacg ttccttcaaa agcgtcaaca ggtaaggcag gccgatcaaa
     3361 tcgcttttat cgacttcgat caggcgcaac cccttgtccg catattcgtg cagcagggct
     3421 ttgaccagcg aggatttgcc tgttccgcgc gcgccgctca tcaatacatt gttcgcgggt
     3481 ctgccgacaa tgaactgttc ggtattacgc accagcaatt cggtttgcct gccgactccc
     3541 gccagccttg ccaaggggaa ggtgtgcgga tcgggcaagt gttcccaaaa accttttttg
     3601 cccgcactct gccagcggaa ggcaagcgcg ttccaatccg tatgcccagg ttcgggcgga
     3661 agcacggcat ccaaacgccg caaaacggca taggctttat cgagaaattc gttcaattcc
     3721 atctctgcct cactttgcat atctttgcgc catcagccgt tcgacggtat cgacgattgc
     3781 ctgcgtattc ggatcgattt cgatgttgat cctgtcaccg acctttctgc tgccgaacag
     3841 cgtccgttcc aaagtttcgg gaatcagatg gacattgaaa cggctgtctt cgactttgcc
     3901 tatggtcagg ctgcaaccgt ccaagccgac gaaccctttg gtcaggatat agggtttgag
     3961 ttcgtgcggg agcgcaaacc aaaccgtgcg gttgtacccg tcccgttcga tttcgacaat
     4021 aggcacggtt gccataatgt gtccgctcat gacgtgtccg ccgatttcgt ctccgaaacg
     4081 cgccgcccgt tcgatgttga cgcaatcgcc ttccttcagc agccctaaat tggtttttgc
     4141 caaagtttcc gccattaaat cgaaactgac acggtttcct tcgatttcgg taatcgtcag
     4201 gcagcagccg ttgttggcga ccgatgcgcc gcgttgcaga ttgtccgccg cctcttgcgg
     4261 aagctcgacg acataagtct gaaatgcctc cgacgggcgg tggattgccg tcagttttcc
     4321 caagccttga acaatgcctg taaacataat cctgtttccc tgtgtcggta aaaatggtgc
     4381 aaattgtagc atctccccgc gaaaaatgcc gtctgaaatg ccttcagaca gcattatgcc
     4441 tccgattcgg gcaaaaaccg cccggtacgg cttgaccttt cctttccacg ccggtcggcg
     4501 gtcttaccct tatccctcct gcaaatcggt ttgcgtgttc aagtcggcaa aatgcccgtc
     4561 aaactcgaat ctgaccgacc gcgccctttg ctgctgcaac cagcttctta atgttttcat
     4621 acccgaaaac agatagggaa tcgcgctttg cagaatctgc gggcggatat acataatgtt
     4681 gtagtgcatc gttatcggcg tttccacata atacgcattg cacaacggtg tgcgtttcga
     4741 cacggtttca aaccttgcca ccaaatcgtc cggcagatac ggcatatcgc acggcacaac
     4801 caaaagccag tcggcagtcg ccaactgcaa atcgttggct gcggtacaca atgccgaaag
     4861 cgggccgaaa tgctgccact gccgtgcgtc cggaaaaata tgcggacttc ttcgagcata
     4921 ttcttccaaa ttccggttgg tgctgatggc gatatggctg acctgcggcc tgaccctgtc
     4981 gatgacatgg tctatcagtg ccttaccccc caaaagagca agccccttgt cctcgcctcc
     5041 catacggctc gcctgaccgt cggccagtat cagggcaaaa gttttcattg cggatgttct
     5101 cttggaaaag ttcgaggttt tcatgattgc agtctgccgt tcccaatgac tcaaaatgcc
     5161 gtctgaagca gacggcaaat aaattcatat tatctgaatt ttatcataac atgatttaac
     5221 actgaagcgg tgcgaattaa gttttcctta accaattctt tctgagcagt tgtatctaat
     5281 tccaaagaat gatattgttt gcattattta gaacaatttt tcgccgagca tgatactgcc
     5341 agcccgtttt tcagacggca tcagcctttc cctacgcctg aaactcctga ccggactgtg
     5401 ggtcgggttg gcggcattgt ctgtcgtttt gacactgctg ctctctttgc gtctggaaaa
     5461 cgcggcctcc gtcatcgaag aggcgggcaa cttgagaatg caggcatacc gtctggcata
     5521 catggcgggt gaaggctcgc cccgtgcgca aattgacaat caggttgccg aatttgaaaa
     5581 aagtttaaaa cgcattgccc aaagcgatgc catccatccg ctgattcctt cggacacccc
     5641 tcttgcttat gatttgatac aatccatgct gattatagat tggcaggcac acatcctccc
     5701 cccgctccag tcctaccggc gaccgactca ggtcgatctc taccgctttg ccggaaacat
     5761 cgaactgttt ttgcaggcat tggaaaatgc caacgaaaaa aacacatggt ggctcaggcg
     5821 ttttcaatgg gcaattatgt tgatgacgct ggtgtcgtct gtactgatgc tgttttggca
     5881 ccagatttgg gttatccggc cgctgcaggc gttaagggaa ggtgcggaac gcatcggacg
     5941 gaggtgtttc gatattccgg ttcccgaagg cggtacgccg gaattcaaac aggtcgggcg
     6001 ttgtttcaat caaatgggcg gcaggttgaa aattttatat gatgatttgg aaggacaagt
     6061 cgccgagcag acacgcagtc tcgaaaaaca aaatcaaaac ctgaccctgc tgtaccaaac
     6121 tacacgggat ctgcaccaat cctacatacc gcaacaggct gcagaacatt ttctaaaccg
     6181 tatcctgccc gccgtaggag cagattccgg cagagtttgt ttggacggcg gatccgatgt
     6241 ttatgtttcc attcatcatg cggattgcgg cacagcagct tcggatttgg ggaagtacca
     6301 tgaggaaatc ttccccattg agtaccagaa cgaaacattg ggcaggctgt tgctcagctt
     6361 tccaaacggc atttctcttg atgaagacga ccgcatcctg cttcaaacac taggcaggca
     6421 attgggcgta tcgcttgccg gcacaaaaca ggaggaagaa aaacgcctgc ttgcagtatt
     6481 gcaggaacgc aacctgattg cgcaaggatt acatgacagc atcgcacaag cattaacgtt
     6541 cctaaaccta caggtacaga tgctggaaac cgcctttgcc gaaaacaaac gggaggaagc
     6601 cgcagaaaac atcggcttca tcaaaacagg cgtgcaggaa tgttatgaag atgtccgcga
     6661 actgctgctc aacttccgta ccaaaatcag caataaagaa tttcccgaag ccgttgccga
     6721 cctattcgcc cgctttacgc aacaaaccgg gataacggtc gaaaccgcct gggaaaacgg
     6781 ttcgttcctg ccgcctcagg aagcgcagct ccaaatgatt tttatcctgc aggaaagcct
     6841 gtccaacatc cgcaaacacg cccgcgccac ccatgtaaaa ttcacccttt ccgaacacgg
     6901 cggacgcttt accatgacca tccaagacaa cggacaaggt ttcgacacgg agaaaatagg
     6961 agaacctacg ggcagccatg tcggactgca tatcatgcag gagcgtgcca aacgtatccg
     7021 cgccgtattg aaaatctatt cccaacccca acagggaacc accgtctcat tggcgggtgt
     7081 atctgaagaa agcttgaaat gaccattaaa attatcctga tagacgacca taccctcttc
     7141 cgcagcggca ttaaagccct tttgtcgcgc caacacggtt ttgaagtcat cggcgaagcc
     7201 gcagacggcc tctcgggcat caaaatgatt agtcggctgc aacccgatgt cgtcctgctc
     7261 gatttggata tgcccggcat gaacggacgc gaagcactct cccaaatcat cagcatcaat
     7321 ccgcagcagg cagtgattat gctgaccgtt tccgaagaca gcgacgactt gaccgaatgt
     7381 attcgcatcg gcgcgcgcgg ttacctgctg aaaaacatca acgctgactt tctgctcgaa
     7441 agcatacgca aagtcgccga aggcgataat gtattctcgc ccgagatgac cgccaaactc
     7501 gttaaaagcc tgatttcccc ccaacctgcc caagggacac aggcactctc ctcacttacc
     7561 cctcgcgaac tggaaatctt gggctatctc gccgcaggac acagcaacaa aatcatcgcc
     7621 cgccacctcg atcttgccga atccaccgtc aaagtccacg ttcaaaacct gctccgcaaa
     7681 ctcaacctca gcagccgggt gcaggccgcc gtttacgcca tccggcacaa cgtcccccaa
     7741 cccgtgctgg aataaggcgg gttcagacgg catattaggg gttttaatcc ccgtacggtc
     7801 attcggataa cagaccacgc atgtaagctt atgcccccat aagtacgctt ggcatagcag
     7861 taatattgtt cggtttagtg ttttccgttt gcccctatct gatactgcaa tatcagctat
     7921 gccgtctgaa aacgcatcat catgatattt tcagacggca taataaaaag cggaaatact
     7981 aatgcagggt aaaatgttcc attccgaatc ccataaatat acaatggctt atatcgttta
     8041 agcatgtgta acccaccctc atatcaatca a
//
LOCUS       DAXHNE010000087         8050 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.087, whole genome shotgun sequence.
ACCESSION   DAXHNE010000087 DAXHNE010000000
VERSION     DAXHNE010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.087"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(176..2218)
                     /gene="recG"
                     /locus_tag="ACFM4Z_001412"
     CDS             complement(176..2218)
                     /gene="recG"
                     /locus_tag="ACFM4Z_001412"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFC6021438.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAIGTHALFSDGVTFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDKLPPGRTPIKTRL
                     VNNVRRAEVEGFVLNTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGELNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQAREIAPILIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(2304..2747)
                     /gene="secB"
                     /locus_tag="ACFM4Z_001413"
     CDS             complement(2304..2747)
                     /gene="secB"
                     /locus_tag="ACFM4Z_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFC6021439.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(2768..3025)
                     /gene="grxC"
                     /locus_tag="ACFM4Z_001414"
     CDS             complement(2768..3025)
                     /gene="grxC"
                     /locus_tag="ACFM4Z_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFC6021440.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHTAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            3239..4732
                     /gene="rng"
                     /locus_tag="ACFM4Z_001415"
     CDS             3239..4732
                     /gene="rng"
                     /locus_tag="ACFM4Z_001415"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFC6021441.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(4767..6017)
                     /locus_tag="ACFM4Z_001416"
     CDS             complement(4767..6017)
                     /locus_tag="ACFM4Z_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6021442.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRIFVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTERGRIAV
                     MQESEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQSAVLQQSIRRNKHLLEQLLALARSQSDETPLAKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVGGDVEIFADETEIYTLVKTFVDNAIRYTPSEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERVRVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGYL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(6013..6188)
                     /locus_tag="ACFM4Z_001417"
                     /pseudo
     CDS             complement(6013..6188)
                     /locus_tag="ACFM4Z_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(6406..7719)
                     /locus_tag="ACFM4Z_001418"
     CDS             complement(6406..7719)
                     /locus_tag="ACFM4Z_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HFC6021443.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
BASE COUNT         1900 a         2368 c         2093 g         1689 t
ORIGIN      
        1 gcgcgggaat gacggcggag ggcttgccgt tttcccgata agtctctgcg gcttcccatt
       61 gccggatttc cgcccacttg ggaataacga gatttaaaat catgaaaatg tgtcaaaaat
      121 aatgcaaaat cagattccgt tgcacttttt tccaatattt ccggcgacat ttcgctcaca
      181 cgcccaaata accttccctg ccggaaagcc atcgtgccaa atgcgcttcg acgatttcgg
      241 ggttttgttc aatcagtatc ggggcgattt cgcgcgcttg ttccaaaagg tgtaagtctt
      301 cttcgagctt ggcgaaacgc agcataggca cgccgctttg acgcgcgccg agaaattcgc
      361 cggggccgcg gatgttgagg tcttggcggg cgatttcaaa gccgtcggtg tgttcgtaga
      421 tgactttcag ccgcgctttg gcgagttcgc ccaagggttc ggcaaacagg aggacgcaca
      481 cgctttctgc cgcgccgcgc ccgacccgac cgcgtaattg gtgcagctgc gccaagccca
      541 tgcgctcggc gtgttcgatg accatcaggg cggcattggg cacatctacg ccgacttcga
      601 taacggtggt ggcgaccaag acgttcagct cccccgaaga aaaccgtgcc atcacttcgg
      661 ccttttcggc ggccttcatg cgcccgtgta ccagtccgat attcagctcg ggcaatgccg
      721 tctgaagccg ggcgagggtt tcggcggcgg tttgcagttg cagggtttcg ctttcttcaa
      781 tcaatgggca gacccaatac gcctgccgcc cttttcggca agtattgagg acgaagcctt
      841 cgacttcggc gcggcggacg ttgttgacga ggcgcgtttt aatcggggtg cgcccgggcg
      901 gcagtttgtc gatgacggac acgtccaaat cggcgaaaaa actcatcgca agcgtgcgcg
      961 ggatgggcgt ggcggacatc atcaactgat ggacttcgcg ccctttgttt ttaagggcga
     1021 ggcgttgggc aacgccgaaa cggtgctgtt cgtccacgat ggtcaggccc aaattgtgaa
     1081 acgtcacgcc gtctgaaaac agggcgtgcg tgccgatggc gattttgacg ctgccgtcgg
     1141 cgagtttggc tttggcttcg tctttggctt ttttacgcaa actgccaaaa aggcggacaa
     1201 cttcaatgcc caaaggttcg agccattgtt taaatttaat aaaatgttgt tcggcaagga
     1261 tttcagtggg cgccattaca gccacctgcg caccggattc gatggcggtc agcgcggaca
     1321 aggcggcgac aatggttttg ccgctgccga catcgccctg cagcaggcgg tgcatcgggt
     1381 aggtttgcgc catatcgcgg cagatttcgg aaacaacttt ttcttgcgca tcggtcaggg
     1441 caaacggcag ggcttggcgc agggcttggg tcaatgtgcc gtcgccgccc aatgccgccg
     1501 ccgtgccgcc gatacgcttc tgtcgcgcca agcgcatcga aagctgttgc gccaaaagtt
     1561 catcgaattt gagccgttgc catgcaggca gcgtgccgtc tgaaagctga tgaatcgtga
     1621 aactcggcgg cggcgaatgc aaaagacgca ggctttcggc gaggtgtggc agcttcagac
     1681 ggcacagcag ggcatcgggc agcgtgtcgt gcagcggcgt aacgtccaac gccgtctgaa
     1741 taatacggcg caaagtgggc tggttcaaac cgtttacggt cgggtaaacc ggcgtgaggc
     1801 tttccgccaa accgccgccc tcggcatcgc ggattttggg atgaatcatc tcgtcgccgt
     1861 aaaagccgtg tttgatttcg cccacggcgc ggatgcgttt gccgaccgcc gtctgtttct
     1921 gatggctggc gtaaaagtgg atgaagcgca gaaaaaggac gctgccggaa ccgtcggcga
     1981 tttggacaat caactgcttg cgcggtttga acgttacttc ctgatggata acctcgccct
     2041 cgacctggca cggcacgcca atcggcgcgt ccttaatcgg cataatgtgc gtctcgtcct
     2101 cgtaacgcag cggcaggtgc aacaccaaat cccacgcggt atggaggttg agtttgtcga
     2161 gcttcttggc ggaaacatcg gtgattttga gctgtttctg ggtttcgggc gacatcatag
     2221 gcagattcct ttgaacgcac ctattttatc cgaaaacaaa aataccgtct gaaacggatt
     2281 cagacggcat cggcaggcag gaatcaagcc ccggcggctt cggcttcctg ctgttgttgg
     2341 taaatcgcct caaaattaat cggcgcgagc aggacgggcg ggaagccggc acgtgttacc
     2401 gtaccggaaa cggcttcgcg cgcgtaaggg aagaggatgt tcggacacgc cacgcccagc
     2461 agcagctgca catcttcttc ggggatgttt tccaaacgga agatgccact ttgggttact
     2521 tcgttcaaaa acatcgtgcg ctcgttatcc agcttggcgg taacggttac ggtaacgtcc
     2581 acgttgtagt agccgtcttc cagcttttgg ctgccggtgg aaacgcgcat atccacttca
     2641 ggctcgccct gttccaaaaa gatttgcggc gcgtgcggca cttccaaaga caagtctttg
     2701 acatacagtc gctcgatgct gaatacgggt tgcagttctt cgctcatttt gttttcctag
     2761 ttgggggtta agggttcagc agtccgtcca gcccgccttc ctgctggagg cggtagaggt
     2821 cggtaaatcc gccgacgtgc gtttcgccga tgaaaatctg cggcacgctg cgctgtcccg
     2881 aaagctgctg catttcggca aaggcttcgg ggcttgcatc gacacggatt tcgtcgatat
     2941 gtccgacacc tgccgtgtgc agcagccttt tcgccatcgc gcagtaggga caaaacggac
     3001 ctgtgtacat ggtaacggtc tgcatattgg gtttccgaaa gttttgcaat gataatcaat
     3061 ataggggcat ttcccctgtt tggcaagtgc ggaacagatg cacgttcaaa cggcatgtgc
     3121 ggaatgtgtc aaagtttctt ttttaaagta tgataggcat tgtgaaaaat atttttgcac
     3181 ccgcgctgcg cggcggaacg ggtgcaaaaa tatttttatt acattttcag gaaaaaccat
     3241 gttgtcagga ctccccatcc ccaaagacat cgcgcgcccg cccgaaacga tattggtcaa
     3301 catcacgccg caggaaacgc gcgtagcggt gttggaggaa aacaatatct gcgagctgca
     3361 catcgagcgc aacagcgaac acagcctggt cggcaatatc tatctgggcg tggtgcgccg
     3421 cgtgctgccc gggatgcaga gcgcgtttat cgacatcggc ttggaacgcg cggcgttttt
     3481 acacatcgtc gatgtcctcg aacaacgccg caaccccgaa gaaacccagc gcatcgaaca
     3541 tatgctgttt gaagggcagt ctgttttggt gcaggtcatc aaagacccga tcaacaccaa
     3601 aggcgcgcgg ctttccaccc aaatctcgct ggcggggcgt ttcctcgtcc atcttccgca
     3661 agaagaccac atcggcgtgt cccaacgcat cgaagacgat gccgaacgca gcagcctgcg
     3721 cgaacgcctc gacaagctcc tgccggaaaa tgcctgccgg ggctacatca tccgcaccaa
     3781 cgccgaaaac gccaccgacg aacagctcca gtccgacatc gactacctga ccaaagtgtg
     3841 ggaacacatc caagaacagg cgaaaatccg gccgccggaa accctgcttt atcaggattt
     3901 gcccttgagt ttgcgcgtat tgcgcgatat ggtcggctgc gacacgcaaa aaatcctcgt
     3961 cgattccacc gtaaaccacg ggcgcatgac gcgttttgcc gaacaatacg tccacggcgc
     4021 attgggcagg atagaattgt tcaaaggcga acgcccgctg tttgaaaccc acaacgtcga
     4081 acaggaaatc agccgcgccc tgcaaccgcg cgtcaacctc aacttcggca gctacctgat
     4141 tatcgaatcc accgaagcca tgaccacgat agacgtgaac accggcggct tcgtcggcgc
     4201 acgcaacttc gacgaaacca tcttccgcac caacctcgaa gcctgccaca ccatcgcccg
     4261 cgaattgagg ctacgcaacc tcggcggcat catcatcatc gacttcatcg atatggcgca
     4321 ggaaagccac cgcgaagccg tgttgcagga gcttgccaaa gccctcgcct tcgaccgtac
     4381 ccgcgttacc ctgcacggtt ttaccagcct ggggctggtc gagctgacgc gcaaacgctc
     4441 gcgcgaaaac ttaaaccaag tcctctgcga accctgccct tcctgccaag gcagaggccg
     4501 tctgaaaacg ccgcaaaccg tgtgctacga aatccagcgc gaaatcgtcc gcgaagcgcg
     4561 ccgttacgat gccgaaagtt tccgcatcct cgccgccccc aacgtcatcg atttgttttt
     4621 ggacgaagaa tcgcaatcct tggcaatgct gatagatttc atcggcaaac cgatttctct
     4681 ggcggtcgaa accgcttaca cgcaggaaca atacgatatt gtgctgatgt aaaaaatgcc
     4741 gtctgaagcc ttcagacggc atctgtctat ttcagggttt ccttgtccaa caaggcgcgt
     4801 atcagcagac cgtgtccgaa cctgcggctg tcggtcagtt ccaaatatcc gccgtatttt
     4861 ttggctagcg tgtcggcaat cgacagcccc agccccgtcc cctgctgctc cgttcccaaa
     4921 atacggtaaa acggatcgag gacgcggacg cgttcggatt ccggaatgcc gttcccgtta
     4981 tcttccaccc acacggcaag atatttccct tcgtctgtga aacccaaatc tatcctgcct
     5041 tcggacgggg tataacgtat tgcgttatca acaaacgttt taaccaaggt atagatttca
     5101 gtttcatcgg caaatatttc gacatcacct ccgaccgcca caccaatgtc ctgacgtttt
     5161 tccaaggcaa gcggcatcag ttcctgcaac acttggcgga aacggctttg cagaccgaat
     5221 gtcgttttcg ccaaaggggt ttcgtccgac tgcgaacgcg ccagtgccaa aagctgctcg
     5281 agcaggtgtt tgttgcgccg tatgctttgc tgcaaaacgg cagactgccg cgccgcatcg
     5341 ggtggcagag acatattgtt gagccgttcc gcctgaaggg aaagggctgt catcggcgta
     5401 cgcaattcgt gtgccgcgtc ggcgacaaac cgctgacggt ggcggatgtc ttcatccgca
     5461 cgtttcaaaa gcaagttgat ggcggttacg aaccctctga tttcactggg aatattgtcc
     5521 acactcaagg cagacaggtc attgattcgg cgttgttcga gactttgcga caatttgcgg
     5581 acggggcgca tggctttgtg cgtaatccac acggtcagca gtatcatcag cggcagtgcc
     5641 gccagcaggg gcaacacgct ttgccgtgcc gcatccgccg ccaaatcttc ccggtattcg
     5701 ctttcctgca tcacggcaat ccgcccccgc tccgtcgtgc ggatatagac gcggtaataa
     5761 tcgtcgtccc cgtccgcctg aagcgtgtgc agaccgtccg ccagatgcgc aggcaggctg
     5821 acaacagggt cttcctgctg cggcatctgc acgaaaatgc gcgtatcgcc gtcgccctcg
     5881 ggcaaagttt cgggtttgga atcgggtgcg acgtacaatg ccgcctgacg gagcaggtcg
     5941 tcctgcaacg cttccgtttc gtggaaggtt tcgtagtagg aaaacatacc tgcaagcatt
     6001 gccagcggaa caaacatcca aaccgtcccg ccccagcaaa cccaaatcca gcagcatcaa
     6061 gtcataaggc tgggcagcgg cagccgcgcg ccgtttttga cccaatccac cgcatagccg
     6121 ccgtctttca aacttgccga caccgcctcc gcaatcatcg catcgtcttc caccagcaaa
     6181 acacgcatca actttccctt caaaataaac cgtgcctatt ctaacacccc aaaattagcc
     6241 gcaatttagc ggcctttacg cttgccggta tttttcaaaa ctgcggcaca aaaaaccgcg
     6301 ccggcaactg ccttcagacg gcattggggc gcgattgcaa cacacgggca ggcagagcct
     6361 gcgacagacc acaggaacga ttcaggcttc agacggcttc gccgtttacg gcagaggcac
     6421 gattcctgcc gctatcgaac tggccaatat cgccagcgac aaaccccacg cccagaaaaa
     6481 cgaataacgg atgtgtttgc ccatcgacaa tttcgccaaa cccaagccca tccacaaagc
     6541 cggcgaaagc ggcgtaacaa aagtgccgac gatactgccg atcaacatcg cataacctgc
     6601 tgcttcgggc gccacgcccg cctgcgaggt aatctgctcc acaatcggaa acagtccgaa
     6661 ataataagcg tccgtactca aaaccaactc aagcggaatg cccaacacac cgatggcaat
     6721 atgcagataa ggcagcagcg cgtccggcag gatatgcaca atgtctttgg aaatcgcgtc
     6781 caacatcccc gcacccttca aaatccccaa aaacgtacct gccgccaaaa taatggacgc
     6841 catcatcacc gcgccgccgg cgtgggcata aatccgctcc atctgttcct gcgggctgcg
     6901 gtaattcaaa agcaacgccg ccgttgcagc cagcataaat acataacccg gcgggaagat
     6961 gcccgaaaaa agcaggctca tcgccgccaa aaacagcagg acattccacc aaaacagttt
     7021 cggacgcgcc aatttttgtt cttcttccga caaaggcacc ggctttatca aatccgccac
     7081 ggcgggcaac gcgcccaact cccggacaat ccgccttttt tcacgcacac ccaaaagcag
     7141 ggacagcgca aggataaaca ccacaccgat aatttgcacc gtcaacaaag gtttatacaa
     7201 ttcgcccaca tctgcgccca acacgcttgc aacccgccca gtcggcccgc cccacggcag
     7261 aaggttaatc aatcccgcac tggaagttag cagcaaaaac agcaggtaag gattcatatg
     7321 cagacgcttg taaagcggca aaagggcggg gacgaccaat aaaaacgtcg tcgcacccgc
     7381 cccgtccaac tgcgccacca ccgacaccaa gaccgtcccc acactcactg ccacgatatt
     7441 accccgagtc agcttaatca aaccgcctat catcggacgg aacagcccca catcgttcat
     7501 gattccaaaa aacaaaatgg aaaacataaa cataatcaca atctgcatca ccgatttggt
     7561 gccgcccgaa taaaattctt ttaattggga tacatcaaac cccgccagca acgccccaaa
     7621 cagcggcacc aagattaatg cgatgatggg cgacactttt tccgtcagca gcaaccatac
     7681 gatgaccccg ataatcagca gtccgataaa cgtcagcatc atttctcctt tattttattt
     7741 taaacagaaa accgaccgtg caggcaaaac cgcccacaga cgcgggataa gccttgcatt
     7801 ctactttttt attttgaaac aagtcaatcg gtcatttcct cccatttacg cctgccgcca
     7861 ttcctgtatc cgtccgtcat ttcacggcgg caaccgatac ggaacaaccg gcaaaccggt
     7921 atcgggacgg cgcgggggca ttcatcccgg tgcgccgatt caaacgaaac cgcccctatc
     7981 attgcggagc gcggggcgtg ccgtacacgc gggattttat agtggattaa caaaaatcag
     8041 gacaaggcgg
//
LOCUS       DAXHNE010000088         7908 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.088, whole genome shotgun sequence.
ACCESSION   DAXHNE010000088 DAXHNE010000000
VERSION     DAXHNE010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7908
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.088"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(90..1346)
                     /locus_tag="ACFM4Z_001419"
     CDS             complement(90..1346)
                     /locus_tag="ACFM4Z_001419"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HFC6021444.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEEIEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLAGLKIDL
                     DTGKYLRLNSARHQTDGFFAAVLQRK"
     gene            complement(1383..2240)
                     /locus_tag="ACFM4Z_001420"
     CDS             complement(1383..2240)
                     /locus_tag="ACFM4Z_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HFC6021445.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLTRRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPHGFHAFEPPLNPYGWRGIYIQD
                     WAEYGFKRQEVRYHLLDRMAYLAPARVAETETLNATEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(2301..3116)
                     /locus_tag="ACFM4Z_001421"
     CDS             complement(2301..3116)
                     /locus_tag="ACFM4Z_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HFC6021446.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            3180..3350
                     /locus_tag="ACFM4Z_001422"
     CDS             3180..3350
                     /locus_tag="ACFM4Z_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021447.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVGRYFTRFGRIYPNQINSDFAAP
                     KDCASEPAVCHP"
     gene            3360..4145
                     /locus_tag="ACFM4Z_001423"
     CDS             3360..4145
                     /locus_tag="ACFM4Z_001423"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC6021448.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMTGEDGWLESGDIVAANPKVLAQMLKIISAHV"
     gene            4415..4934
                     /locus_tag="ACFM4Z_001424"
                     /pseudo
     CDS             4415..4934
                     /locus_tag="ACFM4Z_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4939..6140
                     /locus_tag="ACFM4Z_001425"
                     /pseudo
     CDS             4939..6140
                     /locus_tag="ACFM4Z_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685727.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            6264..7814
                     /locus_tag="ACFM4Z_001426"
     CDS             6264..7814
                     /locus_tag="ACFM4Z_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFC6021449.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQEAGFL
                     TVESHQYERGWFTSTETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQAPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFIDSEGQFRFGTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTQPSGKIDVGGKIMFK
                     DMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT         1896 a         1942 c         2203 g         1867 t
ORIGIN      
        1 cgtactgatt tttgttaatc cactataatt gatgaaatgc cgtctgaacg ctttgaaaag
       61 ggttcagacg gcattttgtt caaaccggtt tatttgcgtt gcaatacggc ggcgaagaag
      121 ccgtcggttt ggtgtcgggc ggagttgagg cgcaggtatt tgccggtatc caaatcgatt
      181 ttcaaaccgg caagcagttc ggcgcagttg acgggttcaa attcgggatg ttcggacagg
      241 aaacgttcga cttgcagctc gttttcttcg ggcaggatgc tgcaagtggc gtaaaccaag
      301 cggccttgcg gttttaccag tttggaggca gcatcgagga tgctgtgttg ctgttccaaa
      361 aggttggcca cggtctcggc agattggcgg tatttgaggt cgggattgcg gcgtaaagta
      421 cccaaaccgg agcagggcgc gtccaccaac acacggtcgg ctttgcctgc cagtcgggcg
      481 atacggctgt cgtgttcgct gctgatgcgt tcggggtgga tattggtcag tccggcgcgg
      541 gtcatacgcg gtttgaggtt ggcaaggcgt ttttcggcga tgtcgaaggc gtagattctg
      601 cctttattgg ccatttgcgc accgacagcc aaggttttgc cgccggcacc ggcacagaaa
      661 tcgacaatga tttcgccgcg ttttgcgccc accaataaag caagcagctg gctgccttcg
      721 tcttggactt ccagtgtgcc gtccaaaaac agctcgtgtt tgttaagcgc gattttgttt
      781 ttcaggcgga tgccccaagg cgaataaggc gttgcctctg catcggcact ttcggcttgc
      841 aacaatggca gcactttatc gcgtttgcct ttcaaggtat tgacgcgtat atcgagcggc
      901 gcagcttggt tgatgctgcg gccgaaggcg aggatttctt cttcgcgcca atgctgctgc
      961 agctgttcta ccagccattg cggcaattct gcggcggtat tcaggccgtc tgaaaactcg
     1021 gttttacggg ctttcaaatt gccgagaaac tctatttctt cttcatcaag caggtctttg
     1081 atttggctga tgttggtgct tctgccgaga accagtgcgg cgagtgcggc tttgcgcggc
     1141 tgcgcgtgcg gacggcgcag ggcggtactg attttttgat agtggcgcag cgcggcaaag
     1201 gcggtttcgg cgatttcgtg gcggtcttgg cggccgagct ttttatgttc gcggaaatag
     1261 gcggagagga cggcatcggc aggctgtttg aaagtcagca tttcagccaa aactttggcg
     1321 gtatggtcga gttgtgcggc gttcataatg taatgggttt ctcttaaagt tggtaaatgg
     1381 tttcagacgg ccttcataat gcgttcgatt tcgtgccaaa agccgtccgg ccgacattcc
     1441 aaaacggcaa tcaagccttg gtcgatacgg cggatttcgg ttgcgttcaa tgtttcggtt
     1501 tcggcgacac gcgcaggcgc gaggtaggcc atacggtcga gcagatggta gcggacttct
     1561 tggcgtttaa acccgtattc cgcccaatct tgaatataga tgccgcgcca accgtatgga
     1621 ttaagcggtg gctcaaaagc atggaaaccg tgtggaaaaa atccgatgcc gcgcacgatg
     1681 gaaacgggtt ttacgtcaag cggcagaccg tgttgccgca aggttcggat gccgtctgaa
     1741 gtgtgcggca gaccgagttg ggcggtcagt ttggcggctt ttcccaacag cgtgtctttg
     1801 gggtcgagtc cgcgcatgtc ttcgagcctg cccgtgccgc cgccgtaata tttgcaggtc
     1861 agctcgatat ggtagggctt gccgttaagc cttaccacaa aatccgccgc gccttgcgta
     1921 ttgccgtccg aaccggaaac cgtgaggttg tgcgcgagca gttcggcgtg tggcgcgttg
     1981 gcaaaccaaa atgccagcag ttcttcggcg taaatgccga ggcggtgtcc gaacggggcg
     2041 cgtcgggtga ggtgttccgt cagcggcgcg ggatcggcat ccaatgccaa aagatagcgg
     2101 aaaccgcgtt cccccaatag ttcgcgcacg cccaattcgc aaccgctttg ccacaaaggc
     2161 ggcgcagtca gcagcgaggc gaggtcgcgg acgggttggc tggtgagctt ccaccataat
     2221 gcgtctaggg cgtaattcat gcttatccga atatcagtca tgccgtctga aaagctggaa
     2281 atgttttcag acggccttaa tcagtctttt ttatggatac gatggctgac ggtattgaaa
     2341 aaaccgcgca ggatatcgat gtcttcggtt tgcgtgttgg cgcgtccgaa caggctctgc
     2401 atacggcgca tcagacgctc gccgttgcgg cggttgaaaa agccgatgtc gttcatcacg
     2461 ctttccatgt gggcgaccat gcctttgatt tgctcgtggg tcgcagcgtg gtcttcttgt
     2521 tgaagatggg tcatgggcga accggtttgg ctgaagattt cgtagcacac gacctgcacg
     2581 gcttgggcga ggttgagcga gaaatagtcg ggattgccgt tgatggtcat cagtcggttg
     2641 caggcttgga cttcttcgat gctcaagccg aaagtctcgt tgccgaaaac cagtgccact
     2701 ttctcgcctc ggtttgcggt ctgcagtaat tcggatacca aatcgcgcgg ggtttgcagc
     2761 ggcgcagtaa tttcgcggcg gcggctggtc agggcgcagg cgatggtggt gtcggcaagg
     2821 gcttcgtcca aagaagcggc aatggtggca ttttccaaaa cgtctgccgc gccggaagcg
     2881 aggatgaagc tttcttccgg taatttaaac gattgaggat gctccgggtc aaacacgggc
     2941 gggttttccg tcatcggcgt tgccatcaga tttggggcga cgatggtcag tttgtgcaga
     3001 cccattgttt tcatcgcgcg cgcggcagag ccgatgttgg cgggatggct ggtgcgcgtg
     3061 aggatgatgc ggatgttgtc cagataagcg ggcagggcgg gtttgagagt agtcatgttc
     3121 ggttttcggt cggaatggaa tagggtataa tgcgccgtct ttttcgttcg gacggtcaaa
     3181 tgccgtctga aatgttcttt aacaacgtcg gaaatttaac gttgcgcctg aagcagacac
     3241 gcgtcgggcg gtattttacc cgatttggca ggatttaccc caaccaaata aactccgatt
     3301 ttgccgctcc gaaggactgc gcgtccgaac cggcggtatg ccacccttaa ggaaatctga
     3361 tgaatccgtt tttgaataca gcctttaaag ccgcccgccg tgccggtcag atgatgatac
     3421 gcgccgcggg caatctcgat gccgtcaaaa ccgacagcaa agcattcaac gattttgttt
     3481 ccgatgttga ccgcaattcc gaaatcatcc tggttgaggc tttgaaagaa gcctatccgc
     3541 accacaaaat cacttgcgaa gaaagcggct cccacggtaa agccgcagcc gaatacgaat
     3601 ggattatcga tccgctcgac ggcacgacca acttccttca cggccatccc caatacgcca
     3661 tctctatggc actcctgcac aaaggcgtgt tgcaagaagc tttggtgtac gcccccgaac
     3721 gcaacgacgt ttacatggct tcgcgcggca aaggcgcgtt gctcaatgac cgccgcatcc
     3781 gcgtttccaa ccgcatcgaa ttgaaccgct gcctgatcgg taccggcttt cctgttgtcg
     3841 atcaaagcat gatggacaag tatctggcga ttttgaaaga tttcttggca aaaaccgccg
     3901 gcggccgtcg tgaaggcgcg gcttctttgg atttgtgtgc tgtggcgact ggtcgttttg
     3961 acggcttttt cgagttcaac ctcaaacctt gggatattgc cgcaggcgca ttgattgttc
     4021 aggaagcggg cggtatcgta accgatatga cgggtgaaga cggctggctg gaaagcggcg
     4081 atattgttgc cgccaatccc aaagtgctgg cgcaaatgtt aaaaatcatt tccgcacacg
     4141 tttaaatttc agcccgctcc cgggaatgcg ggaaaacggg cgggtatgcc gtctgaagta
     4201 tcgcgccgat atttcagacg gcatttttac tggcagcggg ggcaggaagg tggaaacggc
     4261 taaatgcccg aaactgccga agatggaaac ccatatctga ttaaacggag aaccctgtac
     4321 gatttgttta tagtggatta acaaaaacca gtacggtgtt gcctcgcctt agctcaaaga
     4381 gaacgattct ctaaggtgct gaagcaccaa gtgaatgggc aaagccaacg cctttaccgc
     4441 accgattacc gtcgtcaatt acgacgaaca agccctcaat aacaccgaag cgcgtacttt
     4501 ggtggatgcc gtagcgaaaa aagacgcttc cgtttggcag ttcggcggcg aaagcaacac
     4561 gctgaccgga ctgtatttca gaggttatca gcttgatgcg cgccaatacc ggtacggccc
     4621 gcctgaccga ccaatacttc cgcactttaa gcatgaacct gtctgcgcga ggcgaatttg
     4681 aaaccggccc ggtaacgcat aactggagta cggctttcga ccgcgttatc cgccagcgga
     4741 aaaccattcg cggaacggca gccggagcta gcaaggtaga agtgaaggca aacggaaata
     4801 ttgagcatca attggcatcg tttacagccg actatcccaa ttcttgggag aactctgcca
     4861 acttggatgc caatatcaaa gtcaacagcc tggctttgtc cgacaccttg ggcttcgtgg
     4921 ataacaaata ccgttggcgc gcccgatggt aaaaccaacc tgctgccttc ttggaactgg
     4981 caaaataccg ccggccaaac caatatgctt acgtttgaat gggatgcgtt tgacaatgcc
     5041 caaattaccg gcggtatcgg ctacaacaag gcgcgttatt acggcacgct gatttccccg
     5101 accgtttgcg gtacaagcgg tgcgagcgac caaaccgcta cctgctcaag tgccaagcaa
     5161 taccacaccg gcacggcgag cctgaccgac caatatttcc gcacattgag tatgaacctg
     5221 accgcacgcg gcgaatttga aaccggtccg gtaacacata actggagcac cgcgttcgac
     5281 cgtgttatcc gccagcggaa aaccattcag agcggtgatg ccaaacgttt cagcccgatg
     5341 ctgatggcgg cgtgggtgcc gcaacctgat ttggttgtgt acggcaacta tatggaagac
     5401 ttggagcctg ccgatatcaa aaccgatgat tccggcgaaa cgacaatggc gaaaccgcgt
     5461 gtcagccgcc agtttgaaat cggcgtgcgt aaaaactggg gcgattttgt aaccacttta
     5521 aacgcattcc aaatcaaacg cccggctact ggcgcggtca tacaactacc aagacaacaa
     5581 aaggggtaac aacaacgctg acctatggca acaattccga tttcgcccgc tataaggcgc
     5641 aaggcggcgt agccggcgac gagcaaggta tggaacgcaa tcgcggtatc gagttcaatg
     5701 cctatgctaa cctgttaaac aaaaccctgc gtccgacttt gggcctgatg tatctgcaat
     5761 cgaccgtgaa agactaccca aattcacgcg atatgctggt taacggcgta caagtcgcca
     5821 atccgcgcgt aattgccaag gcaggtttgg aatgggatac accgtttgcc aaaggcttga
     5881 ctttaaacag caatgtttcg tatttcggca agtcttacca agacacgcaa aaacaatacg
     5941 ccttcccatc ctataccttg gttgatgtag gcgcgcgcta caaaaccaag ctcggcaaaa
     6001 atacgttgac cgtcagcagc tcggtggaaa atctgttcaa caaaaactac tggcaggtac
     6061 agcgcggcca atacgaccgc agcttcgccg tcgtcggtat gccgcgcact tactggctga
     6121 aggcggaatt ggatttctga tttccctata tccagaaaat gccgtccgaa caggtttcag
     6181 acggcatttc ttttaaagag atatggcggg atacgcttaa ataatgctat tctagcgtct
     6241 ttgaaacctg cttgtttgaa gtgatgaaaa aacctttgat ttcggttgcg gcagcattgc
     6301 tcggcgttgc tttgggcacg ccttattatt tgggtgtcaa agccgaagaa agcttgacgc
     6361 agcagcaaaa aatattgcag gaagcgggct tcttgaccgt cgaatcgcac caatatgagc
     6421 gcggctggtt tacctctacg gaaacgacgg tcatccgctt gaaaccggag ttgctgaata
     6481 atgcccggaa atacctgccg gacaacctga aaacagtgtt ggaacagccg gttacgctgg
     6541 taaaccatat cacgcacggc cctttcgccg gcggattcgg cacgcaggcg cacattgaaa
     6601 ccgagttcaa atacgcgcct gaaacggaaa aagttttgga acgctttttt gggaaacaag
     6661 ctccggtttc ccttgccaat accgtttatt ttaacggcag cggtaaaatg gaagtcagtg
     6721 ttcccgcctt cgattatgaa gagctgtcgg gcatcaggct gcattgggaa ggattgacgg
     6781 gaaaaacggt ttatcaaaaa ggtttcaaaa gctaccgcaa cagctatgat gcgcccttgt
     6841 tcaaaatcaa gctggcagac aaaggcgatg ccgcgtttga aaaagcgcat ttcgattcgg
     6901 aaacttcaga cggcatcaat ccgcttgctt tgggcagcag caatctgacc ttggaaaaat
     6961 tctccctaga atggaaagag ggtgtcgatt acaacgtcaa gttaaacgaa ctggtcaatc
     7021 ttgttaccga tttgcagatt ggcgcgttta tcaatcccaa cggcagcatc gcaccttcca
     7081 aaatcgaagt cggcaaactg gctttttcaa ccaagaccgg ggaatcaggc gcgtttatcg
     7141 acagtgaagg gcagttccgt tttggcacgc tggtttacgg cgatgaaaaa tacggcccgc
     7201 tggacatcca tatcgccgcc gaacatctcg atgcttctgc cttaaccgta ttgaaacgta
     7261 agtttgcaca aatttccgcc aaaaaaatga ccgaggaaca aatccgcaat gatttgattg
     7321 ccgccgtcaa aggcgaggct tccggactgt tcaccaacaa tcccgtattg gacattaaaa
     7381 cttttcgttt cacacagccg tcgggaaaaa tcgatgtggg tggaaaaatc atgtttaaag
     7441 acatgaagaa ggaagatttg aaccaattgg gtttgatgct gaagaaaacc gaagccgaca
     7501 tcagaatgag tattcctcaa aaaatgttgg aagatttggc ggtaagtcag gctggaaata
     7561 ttttcagcgt caatgccgaa gatgaggcgg aaggcagggc gagtcttgac gacatcaacg
     7621 agaccttgcg cctgatggtg gacagtacgg ttcagagtat ggcaagggaa aaatatctga
     7681 ctttgaacgg cgaccagatt gatactgcca tttctctgaa aaacaatcag ttgaaattga
     7741 acggtaaaac gttgcaaaac gaaccggagc cggattttga tgaaggcggt atggtttcag
     7801 agccgcagca gtaatgggtc aaatgccgtc tgaaatgttc aggcggcatt ttgttgtatt
     7861 ggaaactgcc gttttcaacc attcctttag ataagccgtg tcggattc
//
LOCUS       DAXHNE010000089         7882 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.089, whole genome shotgun sequence.
ACCESSION   DAXHNE010000089 DAXHNE010000000
VERSION     DAXHNE010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.089"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..105)
                     /locus_tag="ACFM4Z_001427"
     CDS             complement(<1..105)
                     /locus_tag="ACFM4Z_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFC6021450.1"
                     /translation="MNTNQPAVYDPLTRALHWLTVAGFIGILTTIVLWT"
     gene            383..1537
                     /gene="obgE"
                     /locus_tag="ACFM4Z_001428"
     CDS             383..1537
                     /gene="obgE"
                     /locus_tag="ACFM4Z_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFC6021451.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGETRSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAACPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEARARTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLIEKKRIEAEKAEAERAATNVEIAEQQPKTDTGVFKPE"
     gene            1620..2669
                     /locus_tag="ACFM4Z_001429"
     CDS             1620..2669
                     /locus_tag="ACFM4Z_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6021452.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDTCMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3357..4778
                     /gene="cysS"
                     /locus_tag="ACFM4Z_001430"
     CDS             3357..4778
                     /gene="cysS"
                     /locus_tag="ACFM4Z_001430"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFC6021453.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGATGHTCGHHHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAAFDLSENANDYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAHL
                     AGCLKALGGIIGLLQRDPTEFLQGGAASDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4861..5640
                     /locus_tag="ACFM4Z_001431"
     CDS             4861..5640
                     /locus_tag="ACFM4Z_001431"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC6021454.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGYWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE"
     gene            5627..5971
                     /locus_tag="ACFM4Z_001432"
     CDS             5627..5971
                     /locus_tag="ACFM4Z_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021455.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPIWALVWLLICMPCLLIASKCLGWKGWRLLANVLACLAVCALLSVPAA
                     LLIAFTLQDLLK"
     gene            5981..6511
                     /locus_tag="ACFM4Z_001433"
     CDS             5981..6511
                     /locus_tag="ACFM4Z_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC6021456.1"
                     /translation="MESFELEDLTLWLVRDADEAEMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLQTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVPEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6740..7855)
                     /gene="asd"
                     /locus_tag="ACFM4Z_001434"
     CDS             complement(6740..7855)
                     /gene="asd"
                     /locus_tag="ACFM4Z_001434"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFC6021457.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANDVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWVDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGAETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIETILAGANDWVKVIPNEKEASIRELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
BASE COUNT         2066 a         1984 c         2031 g         1801 t
ORIGIN      
        1 cgtccacagg acaatggtgg tcagaatgcc gatgaagccg gcaacggtca gccagtgcag
       61 cgcgcgtgtc aacgggtcgt aaacggcagg ttggtttgtg ttcataaaaa acttattata
      121 tgtaacggtt tgaacggaca ttccgtcggg tcggaatgtc aaaaaggcgc gattgtacca
      181 aagaagttgg acataatttt gtttgcaggc tgaagatttg cttaaaaatt cattaagatg
      241 ggcggaacaa atagtttggg tacggcgtgt tgaaataccg cttatccctt ataatagcgt
      301 ccgaaattct gttcggacgg catcaaaaca cacggtaatc cgtctgaacc ccccatttga
      361 aaccaacaaa caagagcatt gaatgaaatt catcgacgaa gcaaaaatcg aagtcgccgc
      421 aggcaaaggc ggtaatggcg caaccagttt ccgccgcgaa aaattcgtac cgcgcggcgg
      481 cccggacggc ggcgacggcg gcaaaggcgg tagcgtctgg gcggaagccg acgaaaacac
      541 caacaccctc gtcgaatacc gcttcgttaa acgctaccaa gccaaaaacg gcgaaaaagg
      601 ccacggttcc gaccgttacg gcgcaggtgc ggacgacatc gtcctcaaaa tgcccgtcgg
      661 taccctcatc cgcgacctcg acaccgacga aatcgttgcc gacctcactt accacggaca
      721 gcgcgtctgc ctagccaaag gcggcaaagg cggcttgggc aacatccact tcaaatcctc
      781 cgtcaaccgc gccccaaaac aatccacgcc cggcgaagaa ggcgaaaccc gttccctgca
      841 actcgaactc aaagtcctcg ccgatgtcgg cctgttaggc atgcccaacg ccggcaaatc
      901 caccctgatt accgccgtct ccgccgcatg ccccaaaatc gccaactacc cctttaccac
      961 cctgcatcca aacttaggcg tggtacgcat cgacgaaaac cacagcttcg tgatggccga
     1021 catccccggc ctgattgaag gcgcggcaga aggcgcaggc ctcggccatc gtttcctcaa
     1081 acacttatca cgtaccggcc tgctgctgca cgtcgtcgat ttggccccgt ttgacgaaac
     1141 cgtcaacccc gccgaagaag ccctcgccat catcaacgaa ttgcgcaaat acgacgaaga
     1201 actctacggc aaaccacgct ggctggtgct gaacaaactc gacatgctcg atgaagaaga
     1261 agcccgggcg cgaacagccg ccttcctcga agccgtcggc tgggactacc cgaaaccaga
     1321 cgaccgcttc caattcgaca tggaaacccc gcgcctcttc caaatcagcg cgctgaccca
     1381 ccaaggcacg caggaattgg tacaccaaat caaccaatac ctgatcgaga aaaaacgtat
     1441 cgaagctgaa aaagcggagg cggagcgggc agcgacaaat gttgagattg ccgagcaaca
     1501 gcctaaaacg gatacgggcg tgtttaagcc ggagtgaaaa aagaaagggt ttcagacgat
     1561 atttgttcta caaaaggttg tttgaaacaa ttttaaagca agattgaaat ggattaaaaa
     1621 tgtcatctaa ccaatatatc cagtcgcttg agttgacaaa ctttaccgtt cttccaaacg
     1681 atacttttga attttctgaa aatctcaatg ttattgttgc cgaaaacggt tgtggcaaaa
     1741 cacatttgtt aaaaatttta tacagcctgc tagaagtaac atctaacacc aaaaacagat
     1801 tactgaaaac agaattgcaa aaaagttttg ccgataaatt actgaatgtt ttccgtcccg
     1861 attccttggg caggttgagc aaacgtttgc aggggcgcgg gcgtacggaa atcgttttaa
     1921 aactgcaaaa cggcactacg catagtagtc tgaatttttc cagtaactca tcatcgcaag
     1981 tcaatgttca aagcatcgga ttgaaagaaa atgaatatac tccgacacct atcttcttac
     2041 ccagccgcga attaattaca ctttgccctt ggttcacttc tttgtatcaa aaccaaagca
     2101 tccctttcga agaaacttgg tttgatacct gtatgcaatt aaatcacccg cttgccaaag
     2161 gccccagaga aacaaaaatt cgagaattgc tcgaacctat tgagaacgcg atgggcggaa
     2221 aagtatctga agaacaagga aggttttatc tatcgctgac aaatacgggc ggtaagattg
     2281 aagcaccttt ggtagcagag ggtttgcgga aatttgtcat gattgcccgg ttaatcgcaa
     2341 caggggcatt gctggataaa ggctatctat tttgggatga accggaagct aatctgaatc
     2401 ctaaattgat caaagtggct gcccgtatta tttggtcttt atctcaacag ggtattcagg
     2461 tttttattgc aacacactct ctattcttat tgagagaatt ggaacttttg aagcttgaaa
     2521 ataacacgga tttacctgta cgttttttct cgttgattgc ttccgatgat ggaacaaaag
     2581 tggagcaagg gaacagtatt aatgatttga acacattgat attgttagat gaaaacatta
     2641 tgcaggcaga caggtatctg aatatttgag gttaagctat gactacaatc acagaaaaga
     2701 gattaacctt tgcttttcct gaagattatt acgttaccaa gtatgatgaa tgggaacatt
     2761 ataaaatttt tcagaacagt tgtaatttac gtaataaaat tgatacaaat gaaaagggaa
     2821 agaacgggat aaatcaaagt gtagatgacg ataatggcag ctcaggtgta gatataatag
     2881 cccttcatga atcgacactt tggctaatcg aaattaaaga ttattaccga cttgggcttg
     2941 agccaaatgc ccaatcaata gatgaaaaac tttctgacct accatattta atcgcaagaa
     3001 aaattcgaga ttcattggca ggattggttt ctgctaaatt taaagcagaa aaacaggaag
     3061 aaaaagattt ttctcgcttg gctttagatt gtaacgaaat aaaaatagtc cttcatattg
     3121 aaatgcctag tataagaagt aaactttatc cctcgtcatc agatttagca aacttattaa
     3181 aagataagtt taaattatca gaatttacaa aaaattttgc aaactgttat gcagaaccaa
     3241 ttttcacaaa tatcagccat attaataatc cgcaactacg taatgttcct tggtccgtat
     3301 ctacaggaac agaacagaaa ttatcatccg agcaacaaag atcaatccat agtcccatga
     3361 ccaccatcta caacaccctt acccgccaaa aagaaccctt tgcccccatc gaccctgaaa
     3421 acgtgcgtat gtacgtttgc ggcatgactg tttacgacta ttgccactta ggccatgccc
     3481 gtgtgatggt tgtgttcgac atgattgccc gttggttgcg cgagtgcggc tatccgctca
     3541 cttatgtacg caacatcacc gacatcgacg ataaaatcat tgcccgcgcg gctgaaaacg
     3601 gcgagaccat cggcgagctg actgcgcgtt tcattcaggc tatgcacgaa gatgccgatg
     3661 ctttgggtgt gttgcgtccc gatatcgagc cgaaggcgac ggaaaacatc ccacaaatga
     3721 ttgccatgat tgaaaccctg attcaaaacg gcaaggcata tcctgccgca aatggcgacg
     3781 tttactacgc cgtgcgcgag tttgccgctt acggccaatt gtcgggcaaa tcgttggacg
     3841 acctgcgcgc gggcgaacgt gtggaagtgg acggtttcaa acgcgatccg cttgattttg
     3901 tattgtggaa agcggcgaaa gcaggcgagc cggcatggga aagcccgtgg ggcaacggcc
     3961 gtccgggttg gcacattgaa tgctctgcca tgagcgaaaa cctgttcggc gataccttcg
     4021 acatccacgg cggcggcgcg gatttgcagt tccctcatca tgaaaatgaa atcgcccaaa
     4081 gcgtcggcgc gacggggcat acctgcggtc atcatcacgc gcaaacccac cacgggcaaa
     4141 gcatcgccag ccacgtcaaa tattggctgc acaacggctt tatccgcgtg gacggcgaaa
     4201 aaatgtccaa atcactgggc aacttcttca ccatccgcga agtgttgaaa caatacgacc
     4261 ccgaagtcgt gcgcttcttc atcctgcgcg cccactaccg cagcccgttg aactattccg
     4321 acgcgcattt ggacgatgca aaaggtgcgt tgacccgtct atacaccacc ttgaaaaaca
     4381 cgcctgccgc cgcgtttgat ttgtctgaaa acgccaacga ctacacccgc cgcttctacg
     4441 ccgccatgaa tgacgatttc ggcacggttg aagccgttgc cgtgctgttc gaactggcag
     4501 gcgaagtgaa caaaaccaat gatgcgcacc tcgccggctg cctgaaagcc ttgggtggca
     4561 tcatcggact gctgcaacgc gatccgaccg agttcctgca aggcggcgcg gcttcagacg
     4621 gcctctccaa cgaagaaatc gaagacttaa tcgcacggcg caaacaagcg cgcgcggata
     4681 aaaactgggc agagtccgac cgcatccgcg accttctgaa cgaacacaaa atcattctgg
     4741 aagacaacgc cggcggtacg acttggcggc gcggttaaaa tccatcgaaa agggtaaatg
     4801 tgccgcattt tgcccttttt tgttcagacg gcatttttat tgtgagaact tatttttatt
     4861 atgcttaaaa tcatttccgc caacgtcaac ggcatccgct ccgcctacaa aaaaggtttt
     4921 tacgaataca tcgccgcatc gggcgcggac attgtctgcg tgcaggaact caaagcgcag
     4981 gaggctgatt tgtctgccga tatgaaaaat ccgcatggga tgcacggcta ttggcattgc
     5041 gccgagaagc gcggttacag cggcgtggcg gtgtacagca aacgcaaacc cgacaatgtg
     5101 caaatcggta tgggcattga agaattcgac agggaagggc ggtttgtgcg ttgcgatttc
     5161 gacaggttga gcgttatttc gctttacttg cccagcggca gcagcgcgga agaacgccag
     5221 caggtgaaat accgtttcct tgatgcgttt taccctatgc tcgaagcgat gaaaaacgaa
     5281 ggacgcgaca ttgtcgtttg cggcgactgg aacatcgccc accaaaacat cgacctgaaa
     5341 aactggaaag gcaatcagaa aaattcgggc ttcctaccgg aagaacgcga gtggataggc
     5401 aaggttatcc acaagctcgg ttggacggat atgtggcgca cgctttatcc cgatgtgccg
     5461 ggctacacgt ggtggagcaa ccgcgggcag gcgtatgcga aagatgtcgg gtggcgcatc
     5521 gattatcaga tggttacgcc cgaacttgcc gccaaagccg tgtccgcaca cgtttataaa
     5581 gatgaaaaat tttccgacca tgcgccgctg gtcatggagt atgactatgc tgccgaataa
     5641 agttttgggt aaatatgatt ggaatgtgga cggaaaaaca ggaataggcg cagcatgggt
     5701 tgtcgctgcg tttatcctgc ccatattggt gtgggcgata tttatgttgt cgcggatgca
     5761 aggctggctt gcgccgacca aggcaaaccc gatatgggcg ttggtgtggc tgctgatctg
     5821 catgccctgc ctgctgattg cgtccaaatg tttgggttgg aaaggctggc ggctgttggc
     5881 aaacgttctt gcctgcctcg ccgtgtgcgc tctgttgagt gtgcctgccg cgctgctgat
     5941 tgcgttcacg ctgcaagacc tgctcaaata ggcgcggaca atggaaagtt tcgagcttga
     6001 ggatttgacg ctgtggctgg tacgcgatgc ggatgaagcg gaaatgtgga tagaccgctg
     6061 ggcgatcagt tatcccgtcg tgcagatgtc cgaagcgtcg gccggtcaaa gcatagggga
     6121 atggcaggca gggcttcaga cggcatttga acgcatacgc ggcaaatacg tcgccgttgt
     6181 cgcacacggt gcgggcgcgg ccgcattttt ggcgtggctg tatcaggcag acatcctgac
     6241 acggaagaaa attgccaaca tcatccttgt accgcagcgt cccgatattt ttcccgacga
     6301 tgcggaacac actttccaac gcgtccgctg tccctgccgt gccgcattgg ttgtacccga
     6361 acacggcggc gtgccgcacg gttgggcgca aaaacaggcg gatttgtgga acgcccgcct
     6421 gttggtttcc ccgcattcgg gcagtttgaa cggtatgctc ggcggctggc agtggggtat
     6481 gaagctgatg caggaaatgt tgctggcgtg aacgcttgcc ttatcggaat ttcggctgta
     6541 gcaggaagtc cgcatatcgg gctgttttca ttgacgtgca ggcgcatcag gttcggataa
     6601 agtcgggctg ccgcataccg ccggaaatgg aatcctttat tttttacgtc ggcagtgccg
     6661 gcagggcgtt atccggcagg gttgcaagga aaatgccgtc tgaaacaggc ttcagacggc
     6721 atttccattc aaacagggct cacaggctgc ccaacacgat acgcaatacg cggcgcagcg
     6781 gttcggccgc gccccacaag agctggtcgc cgacggtgaa cgcgctgatg tattcgccgc
     6841 ccatgcccag tttgcggatg cgtccgacag ggacggacag cgtgccggta actttggcag
     6901 gagtcagctc gcggatgctg gcttcttttt cattggggat gactttcacc cagtcattcg
     6961 cgcctgccaa aatcgtttcg atttcggaaa caggcaggtc ttttttcaac ttcagagtga
     7021 tggcttggct gtggcagcgc atcgcgccga cgcggacgca caggccgtca atcacggttg
     7081 gattgtcgct gcggccgagg attttgttgg tttccgcgcc gcctttccat tcttctttgg
     7141 actggccgtt gcccaaatcc acgtcaatcc acggaatcag gctgccggcg agcggtacgc
     7201 cgaagttggc tttcggatag tcttcgctgc gcaggaaatc ggatactttg cggtcgatgt
     7261 cgagaatcga gccggcagga tcggcaagtt cgtccgccac ttgggcgtga accgcgccca
     7321 taccgctgat gagttcgcgc atgtttttcg cacctgcgcc ggaagcggct tggtaggtca
     7381 tgctggttgc ccattcgacc aaatcgtttt ggaacaggcc gcccaaagcc atcagcatca
     7441 gggagacggt gcagttgccg ccgatgtagt ttttcacgcc gtttttgagg ccgttgtcga
     7501 taacgttgcg gttgacaggg tcgaggacga taatcgcgtc gtctttcatg cgcagagaag
     7561 atgccgcgtc aacccagtag ccgttccagc cgctgtcgcg cagggcttgg aacacggatt
     7621 tggtgtaatc gccgccctga caggtaacga tgatgtccat ttttgccaat tcggcaacat
     7681 cgttggcatc caacaatgtt ttggctgcct gaccgaaatc aggggctgcg ccgccgacgt
     7741 tggaagtggt aaagaaaaac gcttcaggaa tgtgggcgaa gtcgttttct tctttcatac
     7801 gctgcatcaa aaccgaaccg accataccgc gccagccgac gaaacctact ttcattgtgt
     7861 tgctccttga ggaaaaaatc gt
//
LOCUS       DAXHNE010000090         7861 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.090, whole genome shotgun sequence.
ACCESSION   DAXHNE010000090 DAXHNE010000000
VERSION     DAXHNE010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7861
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.090"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..1874)
                     /gene="tspA"
                     /locus_tag="ACFM4Z_001435"
     CDS             complement(<1..1874)
                     /gene="tspA"
                     /locus_tag="ACFM4Z_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164729270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark-like protein
                     TspA"
                     /protein_id="HFC6021458.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSAQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     GGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEITFAE
                     VETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLAAAQN
                     GILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESVAQTA
                     ENKPETVDTDFSDNLPSNNHIGTEETASAKPALPSGLAGFLKASSPETILEKTVAEVQ
                     TPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENITETVAETP
                     "
     gene            2182..2712
                     /locus_tag="ACFM4Z_001436"
     CDS             2182..2712
                     /locus_tag="ACFM4Z_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFC6021459.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            2715..3005
                     /locus_tag="ACFM4Z_001437"
     CDS             2715..3005
                     /locus_tag="ACFM4Z_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFC6021460.1"
                     /translation="MEYFMLLATDGEDVHEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAQAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            3005..3283
                     /locus_tag="ACFM4Z_001438"
     CDS             3005..3283
                     /locus_tag="ACFM4Z_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC6021461.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            3332..4198
                     /locus_tag="ACFM4Z_001439"
     CDS             3332..4198
                     /locus_tag="ACFM4Z_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6021462.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            4269..5027
                     /locus_tag="ACFM4Z_001440"
     CDS             4269..5027
                     /locus_tag="ACFM4Z_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6021463.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSEDELHKFYEQQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDALLEENGVKP"
     gene            5100..5591
                     /gene="dtd"
                     /locus_tag="ACFM4Z_001441"
     CDS             5100..5591
                     /gene="dtd"
                     /locus_tag="ACFM4Z_001441"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFC6021464.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            5622..6632
                     /gene="dusA"
                     /locus_tag="ACFM4Z_001442"
     CDS             5622..6632
                     /gene="dusA"
                     /locus_tag="ACFM4Z_001442"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFC6021465.1"
                     /translation="MNDNTHTLPPRHLSVAPMLDWTDRHYRYLARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVKIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGSLILEAWKEVERANM
                     RE"
     gene            complement(6702..7589)
                     /gene="gluQRS"
                     /locus_tag="ACFM4Z_001443"
     CDS             complement(6702..7589)
                     /gene="gluQRS"
                     /locus_tag="ACFM4Z_001443"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFC6021466.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARAHGGKWLIRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQAGARRGADGFVYNGRCRHPGQRPALQGKQPAWRIRVPDRIIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMDKVPKHAVTAP"
     gene            complement(7606..7842)
                     /locus_tag="ACFM4Z_001444"
     CDS             complement(7606..7842)
                     /locus_tag="ACFM4Z_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021467.1"
                     /translation="MFYFSINFSKYTELTKLVSRNASNERIADRAFDFFSPALMDGSA
                     TEEQYNALYDLTLLEEPGMELNKDEIMALINSLK"
BASE COUNT         1712 a         1963 c         2327 g         1859 t
ORIGIN      
        1 tcgggtgttt cggcaacggt ttccgttata ttttcctcaa cggacggtgc ttcggcaggt
       61 tgaagcaatg cggacaaatc gtctgcggcg gcgttgaaat cgggcgtttc aggcgcagtt
      121 tccgcgacgg catcggtttc gtacactttc aggaaatcgt gcaactcttc cggtgtttgg
      181 acttcggcaa ctgttttttc caagatggtt tcgggcgagg aagccttcag gaagcctgcc
      241 agtccggagg gtaaggcagg ttttgcggaa gctgtttctt ctgtgccgat atggttgttt
      301 gagggcaggt tgtcggagaa atcggtatcg acggtttccg gtttgttttc ggcagtttgg
      361 gcgacagatt ccggttcggg cgtgtcgatg acgatttcga cagggttgta cgggttgaag
      421 gtctcgggct cgtacacgct gtctgtggat tcgatggcgt tccaatcggc atccgcgcgt
      481 ttttgggttt cttcatcctg cgtaagtgcg ccggataaaa tgccgttttg cgcggctgcc
      541 aggctgtcga aatccaagtc gatgcggttg gaaggcgtat cggtttcgac atcgaacgtt
      601 tgttttgccg ataactcttc ttcagattcc ccatctaagg caagtgtgtc gtttacatcg
      661 tttttcggag cgggttcggg cgttgccgga gtttcgactt cggcaaaggt gatttctatg
      721 ccgtcgtctg ccgcgtcgtc aaggtcaggc tcttcctcag ggacggattc ttcggtacgg
      781 cgcgcgcgtt tggattgggc aaggcgcaaa agcagcagca gggcgatcaa tgccgcgcct
      841 ccgccggcaa gcagcaaggt gtacgaaccg ccgaacagtc cgctttcggt ttcttcttcg
      901 gcagaaacct gttcgacagg ttcggaaacg gcgttaccgg tttcgtcggt cggcgtgtcg
      961 atggcagaag cggcggcttc ttggggggcg gattcggcag cggtttccga tgcggcagta
     1021 tttgcagcgg gtacaggttc gggtcgaacg gccggttttt ccgcttttgc ttcgggcgcg
     1081 gcaacttttg cttcaggttt ttcaaccggt ttctctaccg ttgcctgttt ggacggttcg
     1141 gacggcatgg atgcggtttc ggctttgggt ttcgccgttt gcggtttggg ttgttccgct
     1201 ttgatcctgt tcagattcgg aatgtgaagc acgctgcccg cacgcagtct gccgtgtgcg
     1261 gaaacatttg ggtttgcctt cagcagcgca tcggcaacct gttcgagcgt caggtgtttc
     1321 gggcggatgg cggcggcaat ctgtttgacc gtttcgcctt tgcggacggt atgggttttg
     1381 ccgttgtacg ccggtttgac ggctgcgttc gcgctgtctt ttttatcggt tttgcggagg
     1441 gctttggcgt tttgattttc ttgggactct gctgtcggag cggttttgcg gtgtgtcttg
     1501 ccgtctgaaa gtgcagattt ggttttgggc gagtagccga caggatcgag gatggcggtg
     1561 tattcgcgta cctgcgcgcc tgcgccgatg cggaacacca ggacgggatc gcggactgcc
     1621 tgtgcggaag aaacggcaat gacggctttg tcgcccaact tgtggacttt ggcggtcagg
     1681 cctttttcgg aaacggtaac gctgccgccg cctagcaggg ctttggcttc ttcgccggtt
     1741 acggtaatgc tgccggaaaa gggttcgtca aggttggact ggatattcag tccgcccagt
     1801 cccgcatgtg cctgaaagga tgcggcaact gcgacggagg cggcaatcag tttgatttgt
     1861 ctgttgtttt tcaagatgta tcccctgtgg gttggcggct gaatacggtt tgaccgcgta
     1921 cagtctgtaa atttcgtcat catcgggcat cggcggggca gtcggccggc gggcatttaa
     1981 tatgtgaatg taccgaccgc cgccacattt taaacggcaa tcattcgccg tttttacaaa
     2041 ttatgacata tctccatctt ttttcaaaaa catctgtgca tatttgcatc aatcaaaaca
     2101 aaatttgttg gttttgcagg tgcaaaaaca gggttctgcc tgtatgatta gcgtttattt
     2161 gatttgcttt ctcatttgga tatgaaattc gtcagcgacc ttttgtccgt catcctgttt
     2221 ttcgccacct ataccgttac caaaaacatg attgccgcaa cggcggtcgc attggttgcc
     2281 ggtgtggttc aggcggcttt tctgtattgg aaatataaaa agctggatac gatgcagtgg
     2341 gtcggattgg tgctgattgt ggtattcggc ggcgcaacca ttgttttggg cgacagccgc
     2401 ttcattatgt ggaagccgag cgttttgttt tggctgggcg cgctgttcct gtggggcagc
     2461 cacctcgccg gtaaaaacgg cttgaaggcg agtatcggca gggagattca gcttccggat
     2521 gccgtatggg cgaaattgac gtatatgtgg gtcggtttcc tgatttttat gggtatcgcc
     2581 aactggtttg tgtttacccg gttcgagtcg caatgggtca actataaaat gttcggctcg
     2641 actgcactga tgcttgtttt ctttattatt cagggtattt atctgagtac ctgtctgaaa
     2701 aaggaggatt gactgtggaa tattttatgt tgctggcaac agacggggag gatgtgcacg
     2761 aggcgcgtat ggcggcacgt cccgaacacc tcaaacggct ggagacgctg aagtcggaag
     2821 gccggctgtt gacggcaggc ccgaatcctt tgccggagga ctccaaccgc gtttcgggca
     2881 gtttgattgt ggcgcagttc gagtctttgg atgcggcgca ggcttgggcg gaagacgatc
     2941 cctatgttca tgcaggcgtg tacagcgaag tgctgatcaa gccgtttaaa gcggtgttca
     3001 aataatgccg gccgtcgatt tgatccgcga acgcctgcag acgctcgatc cgctggtgtt
     3061 ggaaatcggc gatgagagcc atctgcacaa aggacacgcg ggcaataccg gcggcggaca
     3121 ttatgccgtt ttggtcgtta gcggccgttt tgaaggcgta agccgcctga accgccagaa
     3181 aacggtcaaa tcgctgctca aagatttgtt ttcaggcggc atgattcacg cgctcggcat
     3241 ccgggcggct acccctgacg agtatttcca tacggcggac tgaatgaagt ctgcccgaac
     3301 atttcaattt aaaatttaaa gagagaagat tatgaaagca aaaatcctga cttccgttgc
     3361 actgcttgcc tgttccggca gcctgtttgc ccaaacgctg gcaaccgtca acggtcagaa
     3421 aatcgacagt tccgtcatcg atgcgcaggt tgccgcattc cgtgcggaaa acagccgtgc
     3481 cgaagacacg ccgcaactgc gccaatccct gctggaaaac gaagtggtca ataccgtggt
     3541 cgcacaggaa gtgaaacgcc tgaaactcga ccggtcggca gagtttaaaa atgcgcttgc
     3601 caaattgcgt gccgaagcga aaaagtcggg cgacgacaag aaaccgtcct tcaaaaccgt
     3661 ttggcaggcg gtaaaatatg gcttgaacgg cgaggcatac gcattgcata tcgccaaaac
     3721 ccaaccggtt tccgagcagg aagtaaaagc cgcatatgac aatatcagcg gtttttacaa
     3781 aggtacgcag gaagtccagt tgggcgaaat cctgaccgac aaggaagaaa atgcaaaaaa
     3841 agcggttgcc gacttgaagg cgaaaaaagg tttcgatgcc gtcttgaaac aatattccct
     3901 caacgaccgt accaaacaga ccggtgcgcc ggtcggatat gtgccgctga aagatttgga
     3961 acagggtgtt ccgccgcttt atcaggcaat taaggacttg aaaaaaggcg aatttacggc
     4021 aacgccgctg aaaaacggcg atttctacgg cgtttattat gtcaacgaca gccgcgaggt
     4081 aaaagtgcct tcttttgatg aaatgaaagg acagattgcg ggcaaccttc aggcggaacg
     4141 gattgaccgt gccgtcggtg cactgttggg caaggcaaac atcaaacctg caaaataatt
     4201 ctgaaaacgg gatatggcgg caagacgttc agacaggcgt tttgccgccg cgcaggacag
     4261 ggaataccat gaaacagaaa aaaaccgctg ccgcagttat tgctgcaatg ttggcaggtt
     4321 ttgcggcagc caaagcaccc gaaatcgacc cggctttggt ggatacgctg gtggcgcaga
     4381 tcatgcagca ggcagaccgg catgcggagc agtcccaaaa accggacggg caggcaatcc
     4441 gaaacgatgc cgtccgccgg ctacaaactt tggaagtttt gaaaaacagg gcattgaagg
     4501 aaggtttgga taaggataag gatgtccaaa accgctttaa aatcgccgaa gcgtcttttt
     4561 atgccgagga gtacgtccgt tttctggaac gttcggaaac ggtttccgaa gacgagctgc
     4621 acaagtttta cgaacagcaa atccgcatga tcaaattgca gcaggtcagc ttcgcaaccg
     4681 aagaggaggc gcgtcaggcg cagcagctcc tgctcaaagg gctgtctttt gaagggctga
     4741 tgaagcgtta tccgaacgac gagcaggctt ttgacggttt cattatggcg cagcagcttc
     4801 ccgagccgct ggcttcgcag tttgccgcga tgaatcgggg cgacgttacc cgcgatccgg
     4861 tcaaattggg cgaacgctat tatctgttca aactcagcga ggtcgggaaa aaccccgacg
     4921 cgcagccttt cgagttggtc agaaaccagt tggagcaggg tttgagacag gaaaaagccc
     4981 gcttgaaaat cgatgccctt ttggaagaaa acggtgtcaa accgtaatgg catttccaat
     5041 accgatgccg tctgaagcct ttcagacggc attgcacgtt caggtaagga ggacggctta
     5101 tgcgtgcggt catacagaaa acggtaggtg caaaggtgga tgtcgtgtcc gaagccggca
     5161 cggaaacctg tggcaaaatc gacggcgggt ttgtcgtgtt actcggcgta acgcatagcg
     5221 acacagaaaa agatgcacgc tatatcgccg acaaaatcgc ccatttgcgc gtgtttgaag
     5281 acgaagcggg caagctgaac ctgtctttga aagatgtcgg cggcgcggtg ctgctggtgt
     5341 cgcagtttac gctttatgcc gacgcggcaa gcgggcggcg gccttcgttt tcccaagccg
     5401 cacctgcaga acaggcgcag cagctttacc tgcgaacggc ggaactgttg cgcggacacg
     5461 ggattcatgt cgaaacaggg cgtttccgca cgcatatgca ggtgtcgctc tgcaacgatg
     5521 ggccggtaac catactgctg gactctttca tgacgcggat ttccccaaaa atgaaggttg
     5581 ttccggattg aaattgaatc cgcaatgata aaatatcgac aatgaacgac aatacacaca
     5641 cccttccccc gcgccacctg tccgtcgccc ccatgctcga ctggacggac aggcactacc
     5701 gttaccttgc ccgccagatt acccgaaata cttggctgta cagcgaaatg gtcaatgccg
     5761 gtgcgattgt ttacggcgac aaagaccgct ttttgatgtt caacgaaggc gagcagcccg
     5821 tcgccctgca actgggcggc agcgatccgt ccgatttggc gaaagccgcc aaagccgccg
     5881 aggcatacgg ttacaacgag gtcaacctca actgcggctg ccccagtccg cgcgtgcaga
     5941 aaggctcgtt cggcgcgtgt ctgatgaacg aagtcgggct ggttgccgac tgcctcaacg
     6001 ccatgcagga tgcggtcaag attcccgtta ccgtcaaaca ccgcatcggt gtggacaggc
     6061 agaccgaata ccaaaccgtt gccgatttcg tcggcacgct gcgcgacaaa accgcctgca
     6121 aaacctttat cgtccacgcc cgcaacgctt ggctggacgg tctttccccc aaagaaaacc
     6181 gcgacgttcc cccgttgaaa tacgattacg tttaccgcct caagcaggag tttcccgggc
     6241 tggaaatcat catcaacggc ggcatcacca ccaacgaagc aatcgccgga cacctgcaac
     6301 acgttgacgg cgtgatggtc gggcgcgagg cgtaccacaa cccgatggtg atgcgcgaat
     6361 gggacaggct gttttacggc gatacccgca gcccgattga atacgccgat ttggtgcagc
     6421 gtctctacac atacagccaa gcccaaatcc aagccggacg cggcacaatc ttgcgtcaca
     6481 tcgtccgcca cagccttggg ctgatgcacg gtctgaaagg cgcgcggact tggcggcgta
     6541 tgctttccga cgcaacgctc ttgaaagaca acgacggcag cctgattctc gaagcgtgga
     6601 aagaggtcga acgggcaaat atgcgcgaat agggcggggc tgtatgtgtg aaatgccgtc
     6661 tgaaggcttc agacggcatt tgtgcgtttg tcgggcggtg tttagggggc ggtaacggcg
     6721 tgtttcggca ctttgtccat atcccagtgt gccaccgccc agtcgagcag ttcggcaggg
     6781 cggtcggttt ccggtgcttc gggcagcttg aggtaacgga acacttggcg gaggagttgt
     6841 tcgcggcggt ttaaatccaa tgcgggggcg agcgtctgtt tcgaccactt ctgcccttgt
     6901 gcgttggtca gcagcggcag gtgggcatat tgcggtgtcg gaacgcccaa acactgctgc
     6961 aaatagattt ggcgcggcgt ggaaacgagc aggtcttgtc cgcggacgat gtgggtaacg
     7021 ccctgttcgg catcgtcggc aacgacggcg agctggtatg cccagtaacc gtctgcacga
     7081 agcaggacga aatcgccgat gtcgcgggcg aggttttggg cgtaaccgcc gacgatgccg
     7141 tctgaaaaac cgataatgcg gtcggggacg cggatgcgcc acgccggctg tttgccttgc
     7201 agtgcagggc gttggccggg gtggcggcaa cgtccgttat agacgaaccc gtctgcgccc
     7261 cgccttgccc cggcctgcca gtctttgcgg ctgcaatggc agggatagac cagtccggcg
     7321 gttttcaggc ggcatagggt ttcttcatac agggcgtaac ggcggctctg ataggcgact
     7381 tctccgtccc actcgaatcc gaatgcctca agcgtgtgca ggatatggct tgccgccccc
     7441 ggcatttcgc gcggcggatc gaggtcttcc atgcggatca gccatttgcc gccgtgcgcg
     7501 cgcgcatcgg cataggaagc gacggcggtc agcagcgagc cgatgtggag cagcccggtc
     7561 gggctggggg caaaacgtcc tgtgtacata tctggtacag cccctttatt taagactatt
     7621 aatcaaagcc attatctcat ctttattcag ttccatcccg ggctcttcaa gcaaggttaa
     7681 atcatatagg gcattatatt gctcttcggt agctgaacca tccataagag caggcgagaa
     7741 aaaatcaaag gctctatctg caattctctc attacttgca tttctactaa ccagtttcgt
     7801 caattctgta tattttgaaa agtttatgga aaaataaaac agcgaaaaag ttttggtttc
     7861 g
//
LOCUS       DAXHNE010000091         7860 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.091, whole genome shotgun sequence.
ACCESSION   DAXHNE010000091 DAXHNE010000000
VERSION     DAXHNE010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7860
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.091"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..1334)
                     /locus_tag="ACFM4Z_001445"
     CDS             complement(<1..1334)
                     /locus_tag="ACFM4Z_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HFC6021468.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVKNAPSTTRHV
                     EEAAISDGADMLVFLYDTPGLEDAGGVLEWLETHTDTRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQLPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLSGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTYRPETSRTERAGYVEKLQIRLSG"
     gene            complement(1396..2745)
                     /locus_tag="ACFM4Z_001446"
     CDS             complement(1396..2745)
                     /locus_tag="ACFM4Z_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HFC6021469.1"
                     /translation="MLNPSRKLVELVRILEEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRETLERVRAGSFWLWVVAATFAFFAGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAMLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRAVEMLAWLPS
                     KLGFPVPDARAVVEGRLNGNIADARAWSGLLVGSIACYGILPRFLAWAVCKILLKTSK
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALTECGAAWLEPDRAAQEGRLKTNDRT"
     gene            2888..3514
                     /locus_tag="ACFM4Z_001447"
     CDS             2888..3514
                     /locus_tag="ACFM4Z_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFC6021470.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLVLMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIVIYVVMGWMVLAVMKSLTASLPSAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(3578..4996)
                     /locus_tag="ACFM4Z_001448"
     CDS             complement(3578..4996)
                     /locus_tag="ACFM4Z_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6021471.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKG"
     gene            5323..6051
                     /locus_tag="ACFM4Z_001449"
     CDS             5323..6051
                     /locus_tag="ACFM4Z_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFC6021472.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(6110..6598)
                     /locus_tag="ACFM4Z_001450"
     CDS             complement(6110..6598)
                     /locus_tag="ACFM4Z_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HFC6021473.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRGRAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSVAYFLAVALTVWWAWAR
                     AK"
     gene            complement(7356..7820)
                     /locus_tag="ACFM4Z_001451"
     CDS             complement(7356..7820)
                     /locus_tag="ACFM4Z_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFC6021474.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
BASE COUNT         2034 a         2121 c         1974 g         1731 t
ORIGIN      
        1 cccgacaggc ggatttgaag tttctccaca tatccggcgc gttcggtacg gctggtttcg
       61 gggcggtacg tattgagcga cgaccatttc caatgactgc gggctttgtt gagttcgggc
      121 gggagtctgg cggcgtccca cggggctttg cggctgtgca gctcgatatc cgactgtgcc
      181 gcgtgtccgc gcgtttgcag gacgtggagc aaatcgaggg cgcgggcggc gagcagggtc
      241 aaggtttcag ggtcggtgtg caaggtttgg cggccggaga gtttgtcgga aatggtgcgg
      301 gtattgggca ggatgccgcc caaaaagccg ccgattgccg tacccaatcc gagcgagccg
      361 ccgagtgtgg cgatgtccag ccccaagccg atgagcgcgc cggttgccgc gcccgtgccg
      421 gtgcggatgc cgtattgttt gagcaattcg ctgtcgaacg ggtcttggcg gaaggcttgc
      481 ggcatccagt cgccgccgtc gatttcgctg tggtagaaac ggtagagggc aaacagccgc
      541 tgctgcatct gccgttcgag ttggcgtatt tccgcctgca tggtttgcag cacggtggcg
      601 gtatcctcgt tttcgtccac ttcctgcctg aaggcggcgg catcgagcag gaaatcggcg
      661 atttcgcggc gcgcttcgcc gtccagccgc tgccattcgc gccggcgcat ggctgtcagg
      721 cggtcaagtg tgctgcgttc gggcaacatg gtggcgaggt tttcccacag gcgtagttcg
      781 ccttcaaaat caaaggcgac ggtgtcgaac cctgcgaaaa cgtgcaggtt tctcctcgcc
      841 agcatggttg tccacgattc gggaagctgt ccgccggtaa agttgaacac gggcataacc
      901 ggtttggcac accatgaaag gatggtcaat tcatcccgat atttgtcgag gacgggttcg
      961 cgcgcgtcga tgacgtacat tgccatatcg ctttgcaaga cttgccgtaa gactttggct
     1021 tcctgattga aatcatggtg cgcaccgtgg ctgccgagaa actgttgcag ccgctcgatg
     1081 ccgtctgaac gcgtatccgt atgggtttcc agccattcca aaacaccgcc cgcgtcttcg
     1141 agtccgggcg tgtcatacag gaaaaccagc atgtccgcgc cgtcgctgat ggcggcttct
     1201 tcgacatggc gcgtagtgga cggggcattt ttgacttcgc cgaagccgct gtcgcgcaat
     1261 agggtgcgca ggagcgaggt tttgccggta ttggtatgcc cgacgacggc gagggaaagg
     1321 ggttgtttgt tcatgatgtt tttgaagaat ggattttcag acgacctttt aaggatggcg
     1381 gctttatggg gcgtttcaag tgcggtcgtt ggttttcaga cggccttcct gcgccgccct
     1441 gtcaggctca agccacgccg cgccgcattc ggtcagcgcg ttacgccaat gttccagctt
     1501 ttccgaaagg tcgtctgaaa gcccctgttc cgccaaaagc tgcaccaccg cgccgccctg
     1561 cgccgcttcc gaaagtcgga cgatctgccg caacacgccg cggtcgggca cagtttgggc
     1621 gcgcacgccg ataagcagtt gcgccggttt ctgcttcagc tctgtctcca gcgcggcaac
     1681 ctgttcccga ttggcggcaa cgcccttatc cagccattcc tgcgccagcc tgccctcgaa
     1741 ccattcgccg tcctgccatt cggtctccag catgaccgcc catttcggcg catcgttcaa
     1801 gacgattttc ggcgaaacgg cggacacggt ttcccgacgc gtatccgcat cggtgatttt
     1861 gttctgccag cggcggatga ccgcctgata atagggtttt tccaaatcca agccgttttt
     1921 gcttgttttc aaaaggattt tgcataccgc ccaagccaag aagcgcggca ggatgccgta
     1981 gcaggcgata ctgccgacca gcagccccga ccaagcccgc gcatcggcaa tattgccgtt
     2041 cagacgacct tcaactaccg cccgcgcatc ggggacaggg aaaccgagtt tcgacggcag
     2101 ccatgccaac atttccaccg cgcgtaccga agcggcattg ctcaacagcg tgctttccca
     2161 gttgaacgta tattggcgca ccaaaagcag caacaatacc gacaccagca ttccgagcag
     2221 cgtgcagagc cacaggctgt gcgacgttgc gcctattttc caacgtaccg aaggttgccg
     2281 ccactcgtcc gcatacagcc gcaacatcgc ctgattgaca gggtctttgc cccgaaacca
     2341 cgtcgccgga ctgctgaaaa aacgccccac tttcacgcgc aggaacaaca ttgccaacca
     2401 tactgccagc atcagcgtat tcatgcccaa cacgcccgcc aaaaccaaaa agaaattcag
     2461 cccctgattg tccattagaa gataagtgcc tgaaaaaccg gcaaaaaacg caaacgtcgc
     2521 cgccaccacc cacaaccaga acgaccccgc acgcacacgt tccaacgtct cccgcagcat
     2581 acggttcctg tcaatcatct ccgcccgacg gatgattttt tcctccgtac tgccgtccac
     2641 gcggcgcaaa gcctccgtcg cctgcacggg atcgccgctg aaaataaagc cgccttcttc
     2701 caaaatacgg accagctcaa ccagttttcg ggatggattc aacataaaat gccgtctgaa
     2761 aataaaaaac agattttaac acacgcattt tcaagaatat tcacagtgta ggcaaagagt
     2821 aaatcccaca cagaggcaaa agtatcggcg taaactgact gcctctactt tcccgaaaga
     2881 ttgtgcgatg tatacaggcg aacgcttcaa tacttacagc catttgagcg gtttgattct
     2941 ggcggcggca ggtttggtgc tgatgctgct gaaaaccata ggacacgggg acggctaccg
     3001 tatcttcagc gtatcggttt acggcatcag ccttcttctg ctctatttga gttcctcgct
     3061 gtaccacgga attgcagccg gaaaactgaa aagcattttg aaaaaaaccg accactgcat
     3121 gatttatgtc ctgattgccg gaagctacac accgtttgca ctggtttctt tgagaaacgg
     3181 gccgggctgg acggtatttt cactgtcctg gctgctggcg gctgcaggaa tcgcacaaga
     3241 actcaccatc ggacggaaaa gcgaaaaacg tctgctgtct attgtgattt atgtcgtcat
     3301 gggttggatg gtcttggcgg taatgaaatc cctgacagcc tcactcccgt cggcaggact
     3361 ggcttggctg gcggcaggcg gtatgctgta cagcgtcggc atttactggt ttgtaaacga
     3421 tgaaaaaatc cgacacgggc acggaatctg gcatctgttc gtattgggcg gcagcatcac
     3481 ccaatttgtc agcgtgtacg gttacgtaat ctgaatgccg tctgaaaagc aaaacctccc
     3541 gatcctgaag attgggaggt tttctgtttg ccggacatca gcccttgtcg tggaactcgt
     3601 gaaattcata ctgataggac aaatcccgac ccgctttttt ctgtgccaaa taatcatcat
     3661 aaatggcacg gatttcctta cgcaacaaaa acagggctat caagttcggg ataaccataa
     3721 agccgttgaa catatcggac aggctccaaa ccaaatcgac tttgccgagc gtacccaaaa
     3781 cgatggcaag cagaaccaat gcgcgataga tgcccaagtg tcttcccctg aaaagaaaac
     3841 ggatgttgga ctcaccgaaa taataccagc cgataatggt cgtcaaggcg aaaaaggtca
     3901 gacacacggc aagcaattgc gaaccgaagc ccggaaatgc cttgttaaag gcaaattgag
     3961 taaccgccgc gccctgttcg cccgaaaggt tggcatcagt cagcaggata atcaatgccg
     4021 tagccgtaca taccaaaatc gtatcgataa acacaccgac aaatgccgcc ataccttgct
     4081 gcacaggatg cttcacatca gcggttgcat gtgcatgcgg ggtagaaccc atacctgctt
     4141 cgttggaaaa cagaccgcgc gccacgccga aacgtatcgc ttcacgcata ccgatacccg
     4201 cagcaccgcc caaaacggct tcaggattga aggcggcggt aaagatgtgg ttgaacatcg
     4261 gcacaatatg gtcggaaaat tcaaacagga taacgacggc gcacaaaata taaacaaccg
     4321 ccataaacgg cacgacaaac tgggcaatat tggcaatacg gttcacgccg ccaatcacaa
     4381 ccatgcccgc aaggacggca agcacaatac cgactgccaa agaaggcaca tcaaatgcaa
     4441 tggtaacggc agaagcaatg gagtttgcct gtgtcgcatt accgataaag cccaatgcga
     4501 taatcaacgc aatggagaaa aagcccgaca ggaaacgcgc cgcgcccctg ccgattttcg
     4561 gagtcagacc gtgcgtaata taaaacgccg gcccaccgat gtatttgccg tggctgacga
     4621 cgcggtattt ctgcgccagc agtgcctccg caaaaatcgt ggacatcccc aaaacggcag
     4681 aaacccacat ccaaaaaatc gcgcccggcc cgcctgcggt gatggcggtc gccacgccgg
     4741 cgacgttgcc cgtaccgatt tgcgcagata tggcaaccgc caacgcctga aactgcgata
     4801 aagacttgtc gtctttatcg cctttggcaa acaagccgcc gaatacggat ttgaatcccg
     4861 cgcccagctt ggtaatctgc ggcgcaccga gatacagcgt aaaaaacagg ccgataccca
     4921 aaagcgcgta aatcagcagg tagtcccaaa ggaaccgatt gactgtaccc accagaacag
     4981 acaatatatt ttccataaaa taaaccttat cttacaatta aaatgactgc cttccaaaag
     5041 acattccaat aaggaaacac ggcgagcaga ccgtatttgc cgcaacagat gccttaaatt
     5101 gtcaacaatc ggggagaagc tgcgccatca taccgtaaaa tatcgttaat taaaacattt
     5161 ctttattttt aagcggaaag cggaggaaat cgctttcaga cggcatcgac aacggcacgg
     5221 cataaaacag gatattttgg gtacttgcaa cttatgttaa aatgccgacc gtaaaaaatc
     5281 tgacaaaaac agattaatta tttgaaataa gaaaggaaat ttatggcagg ccatagcaag
     5341 tgggcgaata tccagcataa aaaagcccgt caggatgcca aacgcggcaa aatcttcacc
     5401 cgtttaatca aagaaatcac cgttgcggcg cgtatgggcg gcggcgatcc cggcgcaaat
     5461 ccgcgcctgc gcctggcttt ggaaaaagca gccgaaaaca atatgcccaa agacaatgtg
     5521 caacgcgcca tcgacaaagg tacgggcaac ttggaaggcg tggaatacat cgaattgcgc
     5581 tacgaaggct acggcatcgg cggcgcggct ttgatggtgg actgcctgac cgacaacaaa
     5641 acccgcaccg ttgcggacgt acgccacgcg tttaccaaaa acggcggcaa cttgggtacc
     5701 gacggctgcg tggcgttcaa cttcgtgcat cagggctatt tggtattcga acccggcgtt
     5761 gacgaagacg cgctgatgga agcggctttg gaagccggtg cggaagacgt ggttaccaac
     5821 gacgacggtt ccatcgaagt cattaccgcg ccaaacgatt gggcgggcgt aaaatccgct
     5881 ttggaggcgg caggttacaa atccgttgac ggcgacgtta cgatgcgcgc ccaaaacgaa
     5941 accgaactct ccggcgacga tgccgtcaaa atgcaaaaac tgattgacgc actggaagat
     6001 ttggacgacg tgcaagacgt ttacacttcc gccgtattga atttggactg atacgcagca
     6061 cagcagacat acaataaaat gccgtctgaa cctttcagac ggcattgatt tatttagccc
     6121 ttgcccacgc ccaccaaacc gtcagggcaa ccgccagaaa atacgccaca ctccaaacag
     6181 gcaaagcaac ccccagcaga taatccggtt cggcacaatt tccgaacccg cgcacgacag
     6241 gctcgaacca atcaaacaaa ggccagccct tcaatcgaaa cgtccacggc gcaccgcacg
     6301 aaggagccgt acccggcggc agcgactgca gccacaactg atatgccgca acagaaatac
     6361 ccgtaacggc aggaatgctg ataaagacag caccgaacaa accgcctgcc cttcctcttg
     6421 gtctgcacat caggacaatt gccgtacaca atgcggttgc caaaacgcat aaccgctgac
     6481 tgatacacaa aacgcaaggc tccataccca aaacatactg tgccgccaaa gaaccggcaa
     6541 atgcacagac cgaaacggca aacagcagcc aaacggcttt tctaaataac ggggtcattt
     6601 tctcaacaca ccaatcaaaa taccgatatg ccgattttgc tggatatatc ccgagatggc
     6661 aagggacgaa tcgacaccgg cgcaggtgcg ggcgatttcg gttttcccga tatgcacaaa
     6721 acctgacaag ggacaaaacg gcgatttgcc cgcacaatgc ccacaaaaaa atacatccgg
     6781 aggatttgaa tttcagcaaa tttagcgaat caaaagttta tttcaatgaa atcatatgat
     6841 ttttttgaat aagcggattg atgggttttt gaaggaattt gttaccggat agccatcggg
     6901 caagtttttt gcaaaatttg aatcggtcgg gtaaattttc aaaaaataat tgacagcgga
     6961 taagaaacgg cggataattc ccgccgtcga gttgcttgat gcagcttgat ttttctcctc
     7021 tatttctcct ttgtagactt ggcacacatt caactggatg tgtgcatttt tttatctccg
     7081 ctttttttga aatttaatta cttttattgt ttggaattcc attctttaag aaatttaaca
     7141 agaaaaacgc ttgctttttg accggaaaag ccgcataatt cattccgtta gctggttcga
     7201 gtagtcagtt aatagtttct cctctatttc tcctttgtag acttggcaca cattctattg
     7261 aatgtgtgca tttttttatc tgaagcaaca agcctctgtg cgtgatgttg ttatgtttca
     7321 tttaggtgtc aaaccgcata tccggtctga aatattcaat ccaaatccaa aaccggattt
     7381 tctttgacct cctccatcac aacataactc ctactctccg aagcggcagg cagttgcaat
     7441 aagatattgc ctagcatatc ccgataggca gacatatcgg gcaaacgtac tttaatcaga
     7501 tagtcgtatt cgcccgacac caagtggcat tccataattt gcggaatttt cagcacttct
     7561 tttttgaaat cttcgaaaat attgcccgat ttggattgca gcttcagctc gacaaaaacc
     7621 aatagcggtt tgcccaacag atggggattg aggtgggcgt gatagccgga aatataatgt
     7681 tcccgctcca aacggcgcac cctctctgta acgggcgtgg tggacaagcc taccttctcg
     7741 gcaagctccg tcatcgggat gcgggcattc tgttggagga tcttaagaat gcggaaatcg
     7801 attttatcta gttctttcat ttggattgcc ttgtatttat tattgatttt aacaaataga
//
LOCUS       DAXHNE010000092         7800 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.092, whole genome shotgun sequence.
ACCESSION   DAXHNE010000092 DAXHNE010000000
VERSION     DAXHNE010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7800
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.092"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(453..2489)
                     /locus_tag="ACFM4Z_001452"
     CDS             complement(453..2489)
                     /locus_tag="ACFM4Z_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HFC6021475.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTDWANTVERLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSESKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDDGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLNLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKKVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAV"
     gene            complement(2577..3449)
                     /locus_tag="ACFM4Z_001453"
     CDS             complement(2577..3449)
                     /locus_tag="ACFM4Z_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFC6021476.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYVEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            3719..6109
                     /gene="gyrB"
                     /locus_tag="ACFM4Z_001454"
     CDS             3719..6109
                     /gene="gyrB"
                     /locus_tag="ACFM4Z_001454"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFC6021477.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLNV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIETNEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIISDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            6206..7591
                     /locus_tag="ACFM4Z_001455"
     CDS             6206..7591
                     /locus_tag="ACFM4Z_001455"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFC6021478.1"
                     /translation="MISIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNILGNQVLPKHPNSLRFTAYASDGTVLNEQVYYSVGGGFVVTEEDFDRQAETEK
                     AVPYPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRAAAVAEVMEGCIK
                     RGLGADGELPSGLNVRRRVPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
BASE COUNT         1771 a         2156 c         2204 g         1669 t
ORIGIN      
        1 ccgtctgaag cgttgtttga ccgtttcaga cggcattttt atcaaattca aaaatattcc
       61 gtcattcccg cgaaagcggg aatctagaac gtaaaatcta aagaaaccgt tttatccgat
      121 aagtttccgc accgacagac ctggattccc gcctgcgcgg gaatgatggg atttctgttt
      181 ttgatttttt tgtttttggg gaatgacggg atttgagatt gcgggcattt atcgggtaaa
      241 acggaaatta aacgttgcga aaatttatcc ggaatcacag caacttttcc gcgtcattcc
      301 cacgaaagtg ggaatctaga acgtaaaatc taaagaaacc gtttttcccg ataagtttct
      361 gcaccgaccg gtctggattc ccgcctgcgc gggaatgacg gtgcggatgt ttttctatcg
      421 aatccgccat attttttact tcaaccctgc cgtcagaccg cattgtcgaa accgctgtgg
      481 cgcaagagtg cgtctatgct cggttcgcgg ccgcggaagg ctttgaagga ttctgccgcg
      541 ctgcgcgatc cgccgacggc gaggatttcc tgccaaaagc gtttgcctgt ggcggcgaca
      601 tcgtcgcttt cttcaaaggc ggcgtatgcg tccgcgctca atacttccgc ccacgcgtag
      661 ctgtaatagc ctgcggaata gccgcctgcg aagatgtggc cgaagctcaa ggcgaagcgg
      721 ttgtattcgg gcggctggat gacggcgact tttttgcgca cgctgtctaa aacctgttgc
      781 cagtttttca gacggccttc gtcgtcttcg ctgtaaatca tcatatcaaa gagggcgaac
      841 tccatttgcc ggacgaggaa catgccgcgt tggaagtttt tggcggcgag cattttgtcg
      901 aagagttctt tcggcagggg aacgccggtt tcttcgtggg cggacatttg tgccaagaca
      961 ttgtattccc aaacgaaatt ttccataaac tggctgggca gttcgaccgc gtcccattct
     1021 acgccgttga tgccggatac gcccagttcg tccacttggg taagcaggtg gtgcagcccg
     1081 tgtccggttt cgtggaagag gatgaggatt tcgtcgtggc tcaggcgggc ttccctgccg
     1141 ccgacgggtg gggcgaagtt gcagacgagg taggcggtgg gcagttgcag cgtgccgtct
     1201 gaaaaacggc ggcggccttt gtagtcgttc atccacgcgc cgccgcgttt gccttcgcgt
     1261 gcgtacaaat ccatataaac gccgcctatg gtttcgccgt tttgttgcaa ttcaaaatag
     1321 cgcacgtctt tgtgccagac ggggacggtt ttttcggtaa atccgatgcc gtagagtttt
     1381 ttgatttggg cgaacagtcc gtttaatact ttgccgacgg ggaagtattt tttgacttcg
     1441 gtttcgctga acgcgtattt ggcttcgcgc agtttttcgc tggcgtagcc caagtcccac
     1501 ggttgcaaat cggcgaggtt caggctttcg cgggcgaagg ctttgacttc ggcgaggtct
     1561 ttttcggcgt agggtttggc gcggcgggcg aggtcgtgca ggaagtttaa aacttgttcg
     1621 ggcgtgtccg ccattttggt tgccagcgac aattcggcgt agtttttgaa gccgagcagt
     1681 ttggcggttt gcagggcgtt tgcgagcgtg cggtcgatgt tggcggtgtt gtcgaatttg
     1741 ccgtcgtctg aaagttcgct ggcgcgggta acgtaggcgc ggtagatttg ttcgcgcagt
     1801 tcgcggttgt cggcgtattg gatgacggcg aggtagtgtg gaatctgcaa gccgattttg
     1861 tagcctgttt tgctttcgct ttgcgcggcg gcggcaaaca tggcgagcgc gtcttcggga
     1921 atgccggcaa gcggtgcggc atcgtcaaag taaatgccga acgcgtcggt cgcgtctagg
     1981 acgttttggg agaatttggc ggaaagttgc gcgccttcgg tttgcagttt tgccagttct
     2041 gcctgctgtt cgggcggcag ttccgcgccg ctgaggacga aatcgcgcag atcgtggttg
     2101 agtttggttt tttgtgcggg ggagagggtg tcgaattcgg gggaattttt gatggttttg
     2161 aagcggttgt acagctcgat gtcttgtccg atttcggtga agaagacggt gatttcgggc
     2221 atcagttcgt tatagacggc gcgcagttcg ggcgtgtcgg cgacggagtt gaggtgcgac
     2281 accacgcccc aaatcctgcc gacgcgctcg gtgatgccgg tcagacgctc gacggtgttc
     2341 gcccagtcgg tgtgtgtttg tgctttgatg gcggcgattt gttcgcgcgc ttcggcaatg
     2401 gcggtttgca gggcgggttt gatgtcttcg gttttgattt gatcaaaacg gggttcttcg
     2461 cccaaatgga gcagtgcgtt gtcagtcata agatgggttt cctttgcgtg ttgtgcttca
     2521 gacagcatat cggcaatatg ccgtctgaag ccggaaatgg ggtcggtcgg ggaaagttaa
     2581 agtgtttcgg agccggcggg gtaatttaac aggcggtatt gtacgcgcaa aaattcgtcc
     2641 ataaccgcct gttggatttc cagccgcttt gaaacggggg aggcgaagcg gacgatgatg
     2701 cggtatgcct tgtcatcgta cggcacgcgg gtaacgcgcg gtctggcggc gggcgtgata
     2761 aacagttttt ccgcctgcac gttttccaaa tgccgttgga tggcggggat gtagggcgcg
     2821 cacaagggct cgagtacggc tttcagacgg catacggctt catccgaatc caaatggatg
     2881 ggaacgggga tttcgaccgt atggatgaca tagtcgccca aaatattgtc gcggcgcacg
     2941 gggtggctca acaacaggct gttggggaaa gaaacggtgg ttcccgcaag ctgtccgacc
     3001 aaggggttcg gaccgacctg catcatcagc gtgttcaaca ggttgatgtc gaccacgcgc
     3061 ccgcgcaggc cgttgatttc gacatagtcg ccgaccgagt attgctgggt ggcagacctt
     3121 aaaatactgc ccgacagaca cataatcagt tccttcgtcg ccacgacgac cgccgccgcc
     3181 accgcaaaca tcgacaaagc cagcgtttgg atttgcgccg accagataaa tgccagcgaa
     3241 aacagcacca aaagcagcgt tatattgcgg ctggcaacca aaaaccgccg cttgctttcg
     3301 atgccgaaat ccggatgccg tttgaagtgg atattcaaca gaagggcgcg cgccagcagc
     3361 aaagccgcaa ccgccgccac ggattcgacc gcctccgcac gtatcgggac ggcaccgagc
     3421 caagtgtcca acatattcca tatttccatt tccgcgcccc tgtccgaaaa gtatagggtg
     3481 tagattttag tggcaaaaaa acgcttttgc cactttacgg gacaatcccc gacctgatgc
     3541 cggaggcaaa cgtaccgttt catcttttcc cggcggcaca ctgatgcgga taacgctgaa
     3601 ttaggcggca aaatcggcta taatacacaa ttatatatgt cgtcccgccc gctttttcta
     3661 caaggtgcgg acttgttttg gcggcatcgc aaaatctttt caaaatccgg caaaaaatat
     3721 gactgaacaa aaacacgaag aatacggcgc cgacagcatt caggtgctcg aaggcttgga
     3781 agcggtacgc aaacgccccg gcatgtacat cggcgacacg caggacggca gcggtctgca
     3841 ccacatggtg ttcgaagtat tggacaacgc cattgacgaa gcactcgccg gacattgcga
     3901 caaaatcacg gtaacgatac acgccgacca ttccgtcagc gtcgccgaca acgggcgcgg
     3961 tatgcccacc ggcatccacc cgaaagaagg acgctccgcc gccgaagtca tcatgaccgt
     4021 attgcacgcg ggcggtaaat tcgacaacaa cagctacaaa atctccggcg gcctgcacgg
     4081 cgtgggcgtg tccgtcgtca acgcgctgtc cgactgggta acgctgacca tctaccgcga
     4141 cggcaaagaa cacttcgtcc gcttcgtgcg cggcgaaacc gaagagccgc tgaacgttgt
     4201 cggcgattcc gataaaaaag gcacgaccgt gcgcttcctc gccagtacgg aaaccttcgg
     4261 caacgtcgaa tacagcttcg acatccttgc caaacgcatc cgcgaacttt cgttcctgaa
     4321 caacggcgtg gacatcgaat tgaccgacga gcgcgacggc aaacacgaaa gcttcgccct
     4381 ttccggcggc gtggctggat tcgtgcaata catgaaccgc aaaaaaacgc ccttgcacga
     4441 aaaaatcttc tacgcgttcg gcgagaaaga cggcatgagc gtcgaatgcg cgatgcaatg
     4501 gaacgacagc tatcaggaaa gcgtgcagtg cttcaccaac aacatccctc agcgcgacgg
     4561 cggtacgcac ctgaccgcgt tgcgccaagt gatgacgcgc accatcaaca gctacatcga
     4621 aaccaacgaa gtcgccaaaa aagccaaagt ggaaaccgcc ggcgacgata tgcgcgaagg
     4681 tttgacctgc gtgttgtccg tcaaactgcc cgaccccaaa ttttcgtccc aaaccaaaga
     4741 caaactggtt tccggcgaaa tcggtcccgt cgtcaacgaa gtcatcagcc aagccctgac
     4801 cgacttcctc gaagaaaacc ctgccgaagc caaaatcatc accggcaaaa tcgtcgatgc
     4861 cgcccgcgcg cgcgaagccg cccgcaaagc ccgcgaaatc acccgccgca aaggcgtgat
     4921 ggacggtttg ggcctgccgg gcaaactcgc cgactgccaa gaaaaagacc ccgcattgtc
     4981 agaactttat ctggtcgagg gcgattctgc aggcggttcc gccatgcagg ggcgcgaccg
     5041 caaattccaa gcgattttgc cgcttaaggg caagattttg aacgtcgaaa aagcgcgctt
     5101 tgaaaaaatg ctcgccagtc aggaagtcgc cgctttgatt accgcgttgg gcgcgggcat
     5161 cggcaaagaa gaattcaatg ccgagaagct gcgttaccac cgcatcatta tcatgaccga
     5221 tgccgatgtg gacggtgcgc atatccgcac cttgcttttg accttcttct accgccaaat
     5281 gcccgaactg gtcgagcgcg gctacatcta tatcgcccag ccgcctttgt ataaagcgaa
     5341 atacggcaaa caggaacgtt acctcaagga cgagttggaa aaagaccaat ggctgctcgg
     5401 tcttgccttg gaaaaagcca aaatcatttc agacggccgc accatcgaag gcgcagaact
     5461 tgccgacacc gccaaacaat tcctgttggc aaaaaccgtc atcgaacagg aaagccgctt
     5521 cgtagacgaa ctcgtcctgc gcgccatgct acacgcgtcg cccattgatt tgacgtcgtc
     5581 tgaaaacgcc gataaagccg ttgccgaact ttccggtctg cttgacgaaa aagaagtcgc
     5641 cctcgaacgc atcgaaggtc atgaaggaca ccggttcatc aaaatcacgc gcaagctgca
     5701 cggcaacgtc atggtcagct acatcgaacc caagttcctc aacagcaaag cctaccaaac
     5761 cctcacccaa accgccgccg cgctcaaagg catggtcggc gagggtgcca agctttacaa
     5821 aggcgaaaac gggtacgacg cggacagctt tgaaaccgct ttggacatct tgatgagcgt
     5881 tgcccaaaaa ggtatgtcca tccaacgata caaaggcttg ggcgagatga accccgagca
     5941 gctgtgggaa accacgatgg atcccgccgt gcgccgcctg ttgaaagtgc gcatcgaaga
     6001 tgccattgcc gccgacgaag tgttcgttac gctgatgggc gacgaggtcg agccgcgccg
     6061 tgcctttatc gaaaacaacg cgctgattgc ccaaaatatc gacgcataag tgccgtttta
     6121 aaaaaggaga cgggcatcgt gccgcgtctc ctttttggtt ggtcaaacgg aacctgtgcc
     6181 gtctgaaaaa ccgtcggagc aaaatatgat cagcattttc gatattttca aaatcggtat
     6241 cgggccttcc agttcgcata cggtcggccc gatgaaggca gccgccgcct ttgcggcagg
     6301 tttggatgca caggctgttc gcatcgtcat cgacatttac ggctcgctcg cactgaccgg
     6361 atacggacac ggtacatttg acgcgctgat gctcggtttg gaaggcagcc tgccgcacga
     6421 catcccgctt gccggcattc ccgaacgcct cgaacgcatc cgcacgcagc acatcctccg
     6481 gctcaacggg caagaaatcc gcttcatccc cgaccgcgac ctgaacatac tcggcaatca
     6541 agtgctgccc aaacacccca acagcctgcg ttttaccgcc tatgcttcag acggcacggt
     6601 attgaatgaa caggtttatt attcggtcgg cggcggcttt gtcgttaccg aagaagattt
     6661 tgaccggcag gcggaaacgg aaaaagccgt tccctatccc tataccagtt gcgccgaact
     6721 gcttgcccga tgccgtctga accggctcga catctccgaa gtcgtgttgg caaacgaagc
     6781 cgcgcttgcc ggatgcggcg aagccgaaat ccgccgccgc gccgctgccg ttgccgaggt
     6841 tatggaaggc tgcatcaaac gcggcttggg tgcggacggc gaactgccca gcggattgaa
     6901 cgtccgccgc cgcgtcccgc agcttgccgc caagctcaaa gtcctgcgcg aaaccgaaat
     6961 cgtcaacacc cagctctggc cgatggtgta cgccatggcg gtcaacgaag aaaacgccgc
     7021 cggcggacgc gtcgttaccg caccgaccaa cggcgcggca ggcatcattc ccgccgtatt
     7081 gcactatttc cgcaagttca atccgcacgc cacacaggaa cgcgtcgaaa acttcctgct
     7141 caccgcaggc gcaatcggca tcctctacaa gaccaacgcc tccatttccg gtgcggatgt
     7201 cggctgtcag ggcgaagtcg gcgtagcgtg ttcgatggcg gcgggcgcat acgccgaagt
     7261 catcggcggc acgcccaaac aagtggaaaa cgccgccgaa atggcgatgg aacaccattt
     7321 ggggctgact tgcgaccccg tcggcggact ggtgcaaatc ccctgcatcg agcgcaacgg
     7381 catcgccgcc gaaaaagccc tcaaactcgg cacgctcgcg cttttggaag acggcacgga
     7441 caaaaaagtc tcgctcgacg aagtcatccg aaccatgctg caaaccggcc gggatatgaa
     7501 ggcgacctac aaagaaacct cgcttgccgg actcgccgcc acgctccgga aaaaagccgt
     7561 ccccgtatcc gtgcgcgtgg tcgagtgctg aggatggaca gaaacgaaaa tgccgtctga
     7621 aaacagtttt ttagacggca tttcatttat ttcttgcggc tttaccgttt cagtgccagc
     7681 gtcaacacgc ccgccgtaac cagccccaag cctatccatt cctgcgtgtt cgggcgttcg
     7741 tctaagaaaa ccaccgccat caggaatgca aaccggcgtt gtttgcagca tccggcaaga
//
LOCUS       DAXHNE010000093         7786 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.093, whole genome shotgun sequence.
ACCESSION   DAXHNE010000093 DAXHNE010000000
VERSION     DAXHNE010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7786
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.093"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            207..1049
                     /locus_tag="ACFM4Z_001456"
     CDS             207..1049
                     /locus_tag="ACFM4Z_001456"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFC6021479.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVIAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVAV"
     gene            1079..1543
                     /gene="nrdR"
                     /locus_tag="ACFM4Z_001457"
     CDS             1079..1543
                     /gene="nrdR"
                     /locus_tag="ACFM4Z_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFC6021480.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPNCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSAALADMVLKELLRQDTEAAVRFAALHKRFDNPADFASWLAQAVKTGGKA"
     gene            1575..2684
                     /gene="ribD"
                     /locus_tag="ACFM4Z_001458"
     CDS             1575..2684
                     /gene="ribD"
                     /locus_tag="ACFM4Z_001458"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFC6021481.1"
                     /translation="MFSDTDISMMENALRLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALVRAGVSRVVAA
                     MRDPNPLVAGKGLALLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPPNSHLVTDGQSPTYIATLERNEDRLHPYREHAHVRILMPSETADSK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAENLADEIVLYRSPKILGSGKDLFSLL
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNGNAF"
     gene            2879..4297
                     /locus_tag="ACFM4Z_001459"
     CDS             2879..4297
                     /locus_tag="ACFM4Z_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFC6021482.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSSQLVSKLAILLLLLTV
                     GLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIPI
                     ALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFRA
                     IEENAPPARLSATAEFAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPPL
                     FCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACATSFWL
                     FFAFKTESSCRLWQPLKRLPLYTHTLLCLTSSAAYTCFGTPANYPLFAGVWAAYLAGC
                     ILRHRKDLHKLFHYLKKQGFPL"
     gene            4294..5365
                     /locus_tag="ACFM4Z_001460"
                     /pseudo
     CDS             4294..5365
                     /locus_tag="ACFM4Z_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            5385..6549
                     /gene="pglH"
                     /locus_tag="ACFM4Z_001461"
                     /pseudo
     CDS             5385..6549
                     /gene="pglH"
                     /locus_tag="ACFM4Z_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminyl-a1,3-N,
                     N'-diacetylbacillosaminyl-a1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglH"
     gene            6542..7783
                     /locus_tag="ACFM4Z_001462"
     CDS             6542..7783
                     /locus_tag="ACFM4Z_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HFC6021483.1"
                     /translation="MSKAVKRLFDIIASASGLIVLSPVFLILAYLIRKNLGSPVFFIQ
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDSERLTPFGKKLRAASLDELPELWNVLK
                     GDMSLVGPRPLLMQYLPLYDNFQNRRHEMKPGITGWAQVNGRNALSWDEKFACDVWYI
                     DHFSLCLDIKILLLTVKKVLIKEGISAQGEATMPPFTGKRKLAVVGAGGHGKVVADLA
                     AALGRYREIVFLDDRAQGSVNGFSVIGTTLLLENSLSPEQYDVAVAVGNNRIRRQIAE
                     KAAALGFALPVLVHPDATVSPSATVGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHD
                     CLLNAFVHISPGAHLSGNTHIGEESWIGTGACSRQQIRIGSRATIGAGAVVVRDVSDG
                     MTVAGNPAKPLPRKNPKTGTA"
BASE COUNT         1690 a         2551 c         1979 g         1566 t
ORIGIN      
        1 ctcaaaagtt acggaacgaa aaacaaccaa aaccggacaa gtcggattcc cgcctgcgcg
       61 ggaatgacgg aatcttaagt ttccgtcttt gttttctgtt ttctgttttc gagggaataa
      121 tggggaacaa gccgtatttc agacggcatt ttcagttcgg ggtataatcc gaatacttgc
      181 gaccatctga atcattggga caaaccatgt gtcaactgct gggcatgaac tgcaatacgc
      241 cgaccgatat tatgttttcc tttgaaggct tccgccgcag gggcggcatt accgaccacc
      301 atgccgacgg tttcggtatc ggctttttcg aaggcaaagg cgtgcgcctg ttccacgacg
      361 acaagccgag cgcaaattcc cccgtcgccg acctcgtgcg tgcctaccaa atcaaatcgg
      421 aaaacgtcat cgcacatatc cgcaaagcat cgcaaggaca aacctcgctg gcgaacaccc
      481 atccctttat gcgcgaaatg tggggcggct actggctgtt tgcccacaac ggacatttga
      541 ttgatttttt ccccgaacag ggcgaatttt tccaccccgt cggcacaacc gattccgaac
      601 gcgcgttctg ccacatcctc aaccgcctgc gcacccgctt tgccgcccgt cccgacgacg
      661 acacgctgtt tgacgcgatt gcggggctga cgcacgaaat ccgcaagttc gggctgttta
      721 actttatgct ttcagacggc attgccctgt ttgcccacgc cagcacgctg ctgcactaca
      781 tcgtccgcca agccccgttc ggcaaggcgc gcctgctcga cgacgacgtg atggtcgatt
      841 ttgccgaagt aaccacgccc tccgaccgcg tcgccgttat cgccaccctg ccactgaccc
      901 gcgacgaatc atggtcccaa cttgccgtgg acgaactggt catgttccgc gaaggcaaca
      961 tcgtccgaca cgaccgtccc gaaaaccccg tctatatgag tgccgaagaa ggtctggaaa
     1021 tcgcccgcgc cgccggcgtc gccgtctgaa cttcagacga cataggagga cgaacccgat
     1081 gaaatgcccg ttttgcgccc accccgacac ccgcgttgcc gattcgcgtc tgatggaaga
     1141 acgcaacgcc gtgcgccgcc gccgccactg ccccaactgc ggcaaacgct tcggcacgct
     1201 cgaaaccgcc gaactcaaaa tgcccgccgt catcggtccg gacaaaaaac gctcgccctt
     1261 taatgcacaa cgcctccgca acgacctgac cgccgccgcc cgaaaatccg ccctgacacc
     1321 cgaacagatc gacgaaaccg tccgcctgac ggaacacagg ctctacactt cgggtcagcg
     1381 cgacatcccc tctgccgcac ttgccgacat ggtgctaaaa gaactgctcc gacaagatac
     1441 ggaagccgcc gtccgtttcg ccgccctgca caaacgcttc gacaatccgg cagactttgc
     1501 ctcgtggctg gcgcaagccg tcaaaacagg cggcaaagcc tgattccccc aacccatact
     1561 gatacggtat ccctatgttt tcggacacag atatatccat gatggaaaac gccctccgac
     1621 ttgccgcttt ggggcgtttt tccacttcgc ccaatccgcg cgtcggctgc gttatcgcac
     1681 acggcagcca aattgtcggg caaggcttcc acgtcaaagc gggcgaaccc catgccgaag
     1741 tccacgccct gcgtcaggcg ggcgaaatgg cacaaggcgc gaccgccttt gttaccctcg
     1801 aaccgtgcag ccattacggg cgcacaccgc cctgtgccga agcactggtg cgggcgggcg
     1861 tgtcccgcgt cgttgccgcc atgcgcgacc ccaacccgct ggttgcaggc aaagggcttg
     1921 ccctgctcga agcagcaggc atcaagacgg aatgcggttt actcgaacat caggcaaggg
     1981 aactcaaccg aggcttcctg tcgcgcatcg aacgccgccg cccctttgtc cgcctcaaat
     2041 gcgccgtttc gctggacggc aaaaccgccc tttcagacgg cagcagcttt tggattaccg
     2101 gcgaagacgc gcgtgccgac gtacaggttt tgcgtgccga aagctgcgcg gtgctgaccg
     2161 gcatcggcac ggtgttggcg gacaatcccc ggctcaacgt ccgcgctttt ccaactttgc
     2221 gccaacccgc acgcatcgtt ttagacagcc gcctgcgcct gcccccgaac agccatttgg
     2281 ttaccgacgg acaatctccg acctacatcg ccacactcga acgcaacgaa gacagactgc
     2341 acccctatcg ggaacacgca cacgtccgca tcctgatgcc gtctgaaacg gcagacagca
     2401 aaatcgacct gcaccacctg atgcgcctcc ttgctgacga aggtttcggc gaaatcatgg
     2461 tcgaagcagg ctccgaactc acatccgcat ttttggcaga aaatctggca gacgaaatcg
     2521 tcctgtaccg ttcgcccaaa atcctcggca gcggcaaaga cctgttttcc ctgctcgaaa
     2581 accgcgccgc cctttccgca ccgcccttgt ggacacccgt ttcaagcgaa atcctcggac
     2641 acgacatcaa aaccgtgttc cgaaaaaacg gcaacgcctt ttaaagggtt tgcgccgttt
     2701 cactatataa taacgccgat aaaaaacggc cccgttcaga ccgccgcccg cccgaaaaaa
     2761 cgcaacccgg actgccgcac cccgccggca gccgcgacgg tctgaaagcc gtcaaattcc
     2821 gatcaagaaa ggcttcagac ggcacaggca gcatcccgcc gccgcccgga catcaaaaat
     2881 ggacacaaaa gaaatcctcg gctacgcggc aggctcgatc ggcagcgcgg ttttagccgt
     2941 catcatcctg ccgctgctgt cgtggtattt ccccgccgac gacatcgggc gcatcgtgct
     3001 gatgcagacg gcggcggggc tgacggtgtc ggtgttgtgc ctcgggctgg atcaggcata
     3061 cgtccgcgaa tactatgccg ccgccgacaa agacactttg ttcaaaaccc tgttcctgcc
     3121 gccgctgctg tctgccgccg cgatagccgc cctgctgctt tcccgcccgt ccctgccgtc
     3181 tgaaatcctg ttttcgctcg acgatgccgc cgccggcatc gggctggtgc tgtttgaact
     3241 gagcttcctg cccatccgct ttctcttact ggttttgcgt atggaaggac gcgcccttgc
     3301 cttttcgtcc tcgcaactcg tgtccaagct cgccatcctg ctgctgctgc tgacggtcgg
     3361 gctgctgcac tttccggcga acaccgccgt cctgaccgcc gtttacgcgc tggcaaacct
     3421 tgccgccgcc gcctttttgc tgtttcaaaa ccgatgccgt ctgaaggccg tccggcgcgc
     3481 accgttttca tccgccgtcc tgcatcgcgg cctgcgctac ggcataccga tcgcactaag
     3541 cagcatcgcc tattgggggc tggcatccgc cgaccgtttg ttcctgaaaa aatatgccgg
     3601 cctagaacag ctcggcgttt attcgatggg tatttcgttc ggcggggcgg cattattgtt
     3661 ccaaagcatc ttttcaacgg tctggacacc gtatattttc cgcgcaatcg aagaaaacgc
     3721 cccgcccgcc cgtctctcag caacggcaga attcgccgcc gccctgcttg cctccgccct
     3781 ctgcctgacc ggcattttct cgcccctcgc ctccctcctg ctgccggaaa actacgccgc
     3841 cgtccggttt atcgtcgtat cgtgtatgct gcctccgctg ttttgcacgc tggtagaaat
     3901 cagcggcatc ggtttgaacg tcgtccgaaa aacacgcccg atcgcgctcg ccaccttggg
     3961 cgcgctggcg gcaaacctgc tgctgctggg gcttgccgta ccgtccggcg gcgcgcgcgg
     4021 cgcggcggtt gcctgtgcca cctcattctg gttgtttttt gccttcaaga ccgaaagctc
     4081 ctgccgcctg tggcagccgc tcaaacgcct gccgctttat acgcacacat tgctctgcct
     4141 gacctcctcg gcggcctaca cctgcttcgg cacaccggca aactaccccc tgtttgccgg
     4201 cgtatgggcg gcatatctgg caggctgcat cctgcgccac cggaaagatt tgcacaaact
     4261 gtttcattat ttgaaaaaac aaggtttccc attatgaaaa tcgttttgac cacatctatg
     4321 gcaggcttgg gcggcacgga aaccgccacc gtccgcctcg gcaggctact caggcagcac
     4381 ggacacgaca tcatgcttgc ctcttcggac ggaccgttgc tcaaagaggc gcaagccttg
     4441 ggcatacgtt ggcaacccgt cgatttctat cgcggcggcc ttttcggcta cctcaaaggt
     4501 atgattgcct acgcccgaat gctgcggcgc gaacaacccg acatcatcga ctgccaaatg
     4561 gcgcgcgtcg tacccgcctg cgccattgcc gccaaaatcg tttcgccgaa aaccaaaatc
     4621 atctaccact cgcacggttt ggacgcggca acctacccca aaatcgccaa actcttcgac
     4681 aagctgggcg tgtacatcat cggtaactgc aaacacgaac gcgaaaaact catccgccac
     4741 ggtttccccg ccgaccggat tgtctacgcc tacaacgcac tccccccccc cggaatttcc
     4801 tttctggaaa accgtcaaag gctgcgtcat gctcggcacg ctgtcgcgtc tggacaccgt
     4861 ccgcgccgtg catctgatgc tggacatttt caaaaaaatg gtcgaccgcg atatgcccgt
     4921 acgcctgaac gtggcgggca taggcgaaga aatggacaat ttgaaagcgc aggcggaacg
     4981 tctgggcata gacggcaaag ttaccttcct cggcggcgtg cgcgatttga ccgcctactt
     5041 caaagacgtg gatattttgg tgaacacgcc ccactgcatc ggcgaccacg gcgcaggtgt
     5101 gggcaacaac atcttagaag ccggacttta cgacacaccc gtcgttacct acgatatggc
     5161 gggcatttcc gaaatggtcg tcaacgggga aacgggttac tgcatcccct tcggcgacga
     5221 agaagccttt atcgccgccg tagacacgct cgtcagacaa ccgctgttgc gcgaagcaat
     5281 gggcaaaagg ctgcacgcac acgtcgccgc actctgttcc gacgacgaaa tctaccgtac
     5341 caccatggcg gcctatacca tgtaatgccg tctgaaagcc gccgatgaac atcaccatag
     5401 tcgcccctta ttgctcgctg ccgtccgaac cgtatttcaa ccgcttttgg tatctggcag
     5461 aacgattgtc gcaatcgcac gacgtgttgc tgattaccag ccgtttccgc cactacgaca
     5521 aatcattcag acggcatgaa gatgctgccg ccacttcaaa cggcaggctg cgcgtcaagc
     5581 tgctggacga acctggctac cgaaaaaacg tctccctcgc ccgcgtcgcc agccaccgcg
     5641 tattcgtccg caatttggca cgctggctgc acagcccgca ggcggcggaa caggacatcg
     5701 tctattctgc ctatccgctg atggcgacca acctgctgtt gggcaaacac aaagcgcgtt
     5761 tgggctacaa actgattgtc gatgtgcagg acgtatggcc ggagtctttc tcctcggtcg
     5821 taccattttt gaaaaaagta ccgcacaagc tgctgccctt tgcttcacgc gccaaccgtg
     5881 cctaccgctg cgccgacgcg ctggttgccg tatcgcagac ttatctcgac cgcgccaaag
     5941 aagccaatcc gaacgtaccc ggcgaagccg tctatatcgg tacggatttt gccgccatcg
     6001 ccccccccgc gattccgctc caaaaccacc cgccttttct acttgggaac gctcagttac
     6061 agctacgacg tggaaaccgt gtgcaaaggt gttcggaaac ttttagacga cggcgaaaac
     6121 gtcgaattgc acattatggg cggaggaccc gatttggaga aactcaaaca atatgaaaat
     6181 cgcgccatca agttttacgg ttatttaccc tattctgaaa tgatgtcgat tgccaaaggc
     6241 tgcgacattg ccgtcaacgc catccatagc cacgccatgc agtcggttac caacaaactc
     6301 tccgactata tggctttgca aaaaccgatt ctgaacagcc agaccaatgc cgaagtcctt
     6361 agcctcttga acctgctgcc gcacgaaaac taccgttccg gcgacgtgga cagcttcgtt
     6421 caagccgcca aagatatttt gaaacgcaaa aacgaccccg ttcagtccga tgaaatcgtc
     6481 cgccgcttca ggcgcgacat ttcctatcgg aaaatcgtca acctgattga aagattggca
     6541 aatgagtaaa gccgtcaaac gcctgttcga catcatcgca tccgcatcgg ggctgattgt
     6601 cctctcaccc gttttcctga ttttggcgta cctcatccgc aagaatctgg gttcgcccgt
     6661 cttcttcatt caagaacgcc ccggaaagga cggaaaacct tttaaaatgg tcaaattccg
     6721 ttccatgcgc gacgcgcttg attcagacgg cattccgctg cccgacagcg agcggctcac
     6781 acccttcggc aaaaaactgc gtgccgctag tttggacgaa ctgcctgaat tatggaatgt
     6841 cttaaaaggc gacatgagcc tggtcggccc gcgcccgctg ctgatgcaat atctgccgct
     6901 gtacgacaac ttccaaaacc gccgccacga aatgaaaccc ggcattaccg gctgggcgca
     6961 ggtcaacggg cgcaacgcgc tttcgtggga cgaaaaattc gcctgcgatg tttggtatat
     7021 cgaccacttc agcctgtgcc tcgacatcaa aatcctactg ctgacggtta aaaaagtatt
     7081 aatcaaggaa gggatttccg cacagggcga agccaccatg ccccctttca caggaaaacg
     7141 caaactcgcc gtcgtcggtg cgggcggaca cggaaaagtc gttgccgacc ttgccgccgc
     7201 actcggccgg tacagggaaa tcgtttttct ggacgaccgc gcacaaggca gcgtcaacgg
     7261 cttttccgtc atcggcacga cgctgctgct tgaaaacagt ttatcgcccg aacaatacga
     7321 cgtcgccgtc gccgtcggca acaaccgcat ccgccgccaa atcgccgaaa aagccgccgc
     7381 gctcggcttc gccctgcccg ttctggttca tccggacgcg accgtctcgc cttctgcaac
     7441 agtcggacaa ggcagcgtcg ttatggcgaa agccgtcgta caggcaggca gcgtattgaa
     7501 agacggcgtg attgtgaaca ctgccgccac cgtcgatcac gactgcctgc ttaacgcttt
     7561 cgtccacatc agcccaggcg cgcacctgtc gggcaacacg catatcggcg aagaaagctg
     7621 gataggcacg ggcgcgtgca gccgccagca gatccgtatc ggcagccgcg caaccattgg
     7681 agcgggcgca gtcgtcgtac gcgacgtttc agacggcatg accgtcgcgg gcaatccggc
     7741 aaagccgctg ccgcgcaaaa accccaagac cgggacggca taagcg
//
LOCUS       DAXHNE010000094         7705 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.094, whole genome shotgun sequence.
ACCESSION   DAXHNE010000094 DAXHNE010000000
VERSION     DAXHNE010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7705
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.094"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            344..568
                     /locus_tag="ACFM4Z_001463"
     CDS             344..568
                     /locus_tag="ACFM4Z_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6021484.1"
                     /translation="MKVISVLNQKGGSGKTTVATHLARALQLAGNSILLVDSDPQGSS
                     RDWAAVHEEQPVPVIGIDSPTIDRDLKSVV"
     gene            584..988
                     /locus_tag="ACFM4Z_001464"
     CDS             584..988
                     /locus_tag="ACFM4Z_001464"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HFC6021485.1"
                     /translation="MDGAPQTADLAISAIKASDLVLIPVQPSPYDIWATADLVNLIKQ
                     RIKLIDGNLKAAFLINRTIKGTKIGGEIRAALEEYELPVLSTVIVQRIIYPTSAITGS
                     TVLDKEPNGEAAKEIQSLANEIINLINIDNHI"
     gene            1031..1234
                     /locus_tag="ACFM4Z_001465"
     CDS             1031..1234
                     /locus_tag="ACFM4Z_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HFC6021486.1"
                     /translation="MNGLKADRPSANKANLSIDDVIKQEEIKRISFDMSESLHRELKK
                     YAAENGVTIRVVLNKLVSDLLNK"
     gene            1410..2573
                     /locus_tag="ACFM4Z_001466"
     CDS             1410..2573
                     /locus_tag="ACFM4Z_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC6021487.1"
                     /translation="MHINFSMEYKEFNENYLYKKPFIFKNALDVSSISWKEINELYQR
                     ADPTDWQFKFRKGEIIPKEAYVESFNDVGRIRHRFNKTAVYQYLQDGATMVYNRIDNE
                     PFVDSIAKQIAQFAQAQTVVSGYLAFGSSPSYRNHWDTRDVFAVQLIGKKHWTVSAPN
                     FDMPLYMQQAKDMPHITPPTTVDMEVILEAGDILYIPRGWWHNPMPMNCETFHLAIGT
                     FPPNGYNYMEWLMKKIPDIQSIRQNFIDWEHDQKNIDNAAQAVTEMMKNQENYQAFIQ
                     DFLGNQRVNTAFNMQIFGNLDNDRLPENSTIKLNSLDNRTIKQGYIIANGIKTNLDND
                     SQTILQWIADKHSVKLTQLYEFCQNQNINLEKVEKLVFDLTMIDVLECLTDER"
     gene            2580..2786
                     /locus_tag="ACFM4Z_001467"
     CDS             2580..2786
                     /locus_tag="ACFM4Z_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021488.1"
                     /translation="MFTFLNKNIFIKTIVLGCFYSFLFFSFFVSGKSFGYSFEIFNMD
                     TSALAFLSVFMGFFISVAVHIYDI"
     gene            2810..3145
                     /locus_tag="ACFM4Z_001468"
     CDS             2810..3145
                     /locus_tag="ACFM4Z_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HFC6021489.1"
                     /translation="MKELTINDLTLVSGGFEFGRDYIEIAPVVGTAGGVASSVRAASM
                     TGEAGAILTRSGVTGLRAGVVAIPLLAYNGTVWVNRNTKLQDNLGRLIDKVTGLDQSG
                     NDYGDGTSY"
     gene            3180..5275
                     /locus_tag="ACFM4Z_001469"
                     /pseudo
     CDS             3180..5275
                     /locus_tag="ACFM4Z_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
     gene            5277..6539
                     /locus_tag="ACFM4Z_001470"
     CDS             5277..6539
                     /locus_tag="ACFM4Z_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFC6021490.1"
                     /translation="MTLFRPEVFQAKKNRWTGQIVLVRPFSLQFLTFFAVSLAAILVA
                     FLIFGSYTNKTTVTGQLLPTTGVVRVYSQDMGVIAHQHVMNGDFVKKGDVLFTLSTSR
                     NDNNGSIQARLLAEAELKKSLSEQEIIMKKRVHAAEKTAQENTVHRFQNQMQHVRNQI
                     IMQEKRIAISEKMLEKQRYLAKMDAISELEKNSYEIALLELKAGLAAYQREADNLARE
                     ITVQQSNLKNLPEQQATEISQLERAVSVYQQEILDYQQRNEQTIRATISGYVSSINTE
                     IGQQVDTNKLLMSIVPKESELLANLYVPSRAIGFVKPNDKVILRYQAYPYQKFGHAEG
                     HVISIAQTALGAQEWTNLGNIFTQTAQVNEPVYLIKVKLDNQHIRIYGTEKKLQIGMI
                     LEADILHENKRLYEWILDPLYQVMGKIS"
     gene            complement(6809..7648)
                     /locus_tag="ACFM4Z_001471"
     CDS             complement(6809..7648)
                     /locus_tag="ACFM4Z_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021491.1"
                     /translation="MGNFAKVSNCEFWDKINYNRAAQAAEQRAKQAQETPKEPPKQEE
                     HFIAKLPNDKLMTIYRLFQKQLDDMRGLERDIERGDWYINARYRMNENRNKIRALEDD
                     NKRLHHDYWAVQDQGENRFFGMGKIGAKLVKENEKQQHINERHGANVREIRRLKTENE
                     KLEGQYSDKLAADVVKVRDRFNEELDNVRREIGKRPMEEFRNVFLQAAQEAQTTIGQK
                     AVQIELEKFPLNGSPSAKRIAFYFGAQRIREVEQKEREDQRKEQERQARRMERDDGHG
                     IGM"
BASE COUNT         2325 a         1373 c         1577 g         2430 t
ORIGIN      
        1 gcggcccgga tgcctgatta tggggcgtcc ggggaggatt aagggggcat ttgggtaaaa
       61 tcaggagtaa ttaggagcgg aaatagacga aaacctgtgt ttgggtttcg gctgttgggg
      121 gaagggcttt tttgcaaagg tctcaattta aactttaacg caatatcaac tcattttctg
      181 ccacatatct caaaaaagtc tagtttgata cagtaatctc tttaacagct tttcttgcta
      241 aaatttaaaa caaaatgtaa atattctttt atttatttaa ataatagatt aaaatattat
      301 ttaattaaaa gcataatgag cgtagatatt caaaggagct aacatgaaag ttatttccgt
      361 attaaatcaa aagggtggaa gtggtaaaac cacagtcgca actcacttgg cacgcgcctt
      421 gcaattagca ggaaatagca tcttattggt tgatagcgat ccacaaggaa gcagtaggga
      481 ctgggctgct gttcacgaag agcaacctgt tcctgtgatt ggtattgaca gcccgacaat
      541 tgatcgtgat ttaaaaagcg ttgtttaaaa agattttgtt attattgatg gcgcaccaca
      601 aacagccgat ttagccatat cagcgattaa agcctctgat ttagttttaa tccctgttca
      661 accgtcgcca tacgatattt gggcaaccgc tgatttggtt aatttaatca agcagcggat
      721 aaaactcata gatggaaatt taaaggctgc ttttttaatt aaccgaacta ttaaaggaac
      781 aaagattggc ggagaaatta gagcagcgtt agaagaatat gagttacctg ttttatcaac
      841 cgtgattgtg caacgtatta tttatccaac atctgctatc acaggtagca ctgtactgga
      901 taaagaacca aatggagaag cagctaagga aattcaatca cttgcgaatg agataataaa
      961 tttgataaat atagataatc atatttaatg aaatattatt ttaaattttt aaatattgta
     1021 ggaaataatt atgaacggat taaaagcaga tcgtcctagt gcaaacaaag caaacttatc
     1081 tattgatgat gtgatcaagc aagaagaaat caagagaatc agttttgata tgtctgaatc
     1141 tctgcataga gaattgaaga aatacgcggc tgagaatggt gtaactattc gcgtcgtgct
     1201 caataaactc gtttcagatt tattgaataa ataataacac acggcagaat gtatttgacc
     1261 ataatgtcaa agcaatatcc aactttatct aaaaataaca aaataccttc ttccaaaaac
     1321 agaacgtctt ttcattgcaa aacttgacat ataaacacta gttaagtaaa cttaattttc
     1381 attattaata atgaaaataa ggattatttt tgcatattaa cttttcaatg gaatataaag
     1441 aatttaatga aaattattta tataaaaagc catttatttt taaaaatgct ttagatgtta
     1501 gttctatctc atggaaagaa attaatgagt tgtatcaacg tgcagaccct actgattggc
     1561 aatttaaatt tcgcaaaggg gaaataatcc ccaaagaagc ctatgttgaa tcattcaatg
     1621 atgtgggtag aatacgtcat cggtttaata aaacagccgt atatcagtat ctgcaagatg
     1681 gtgcaacaat ggtttataac cgtattgata atgagccatt tgttgacagt attgctaaac
     1741 aaattgccca gtttgctcaa gcacaaactg ttgttagtgg ttatttggcg tttggtagtt
     1801 ctccatcata tcgtaatcat tgggatactc gtgatgtgtt tgcggttcaa ttgattggca
     1861 aaaaacactg gacagtttct gctccaaatt ttgatatgcc attgtatatg cagcaagcaa
     1921 aagatatgcc gcacattact ccgcctacaa cagtggatat ggaagtcatt ttagaagcag
     1981 gagatatttt atatatcccg cgtggttggt ggcataatcc tatgcctatg aattgtgaaa
     2041 cattccattt agctattgga acttttccgc caaatggtta taattatatg gaatggttga
     2101 tgaaaaaaat acctgatatc cagagtattc gtcaaaattt catagactgg gaacacgacc
     2161 aaaaaaatat agataatgcg gctcaggcag ttactgaaat gatgaagaac caagaaaatt
     2221 atcaagcatt catacaagat tttttgggaa atcaacgcgt aaatactgca tttaatatgc
     2281 aaatttttgg aaatctggat aatgacagat tacctgaaaa tagtacaatt aaactcaatt
     2341 cgttggataa ccgtacaatt aaacaaggat atatcatagc gaatggcatc aaaaccaact
     2401 tggacaatga tagccaaacg attttgcaat ggattgctga caaacatagt gttaagttaa
     2461 ctcaactgta tgaattttgc caaaatcaaa acattaattt ggaaaaagtg gaaaaattag
     2521 tgtttgattt gacaatgatc gatgtattgg aatgtttaac tgatgaaaga taaattatta
     2581 tgtttacttt tttaaataaa aatatattta tcaaaacaat tgtacttggc tgtttttatt
     2641 cctttttatt ttttagtttt tttgtatctg gtaagtcatt tggttattca tttgaaattt
     2701 ttaatatgga tacaagtgcg cttgcttttt taagtgtatt tatgggcttt tttataagcg
     2761 tggctgtgca tatttacgat atttaataca attgaaaaga taaataatta tgaaagaatt
     2821 aactattaat gatttaaccc tagtttcggg tggatttgaa tttggtcgtg attatattga
     2881 aatagctcca gttgtaggta cagcaggtgg cgttgcctct tctgtgagag cagcttcaat
     2941 gactggagaa gctggagcaa ttcttactcg ttcaggagta acaggattac gtgctggagt
     3001 ggtagctatt ccattgttag cctacaatgg tactgtatgg gttaatagaa ataccaaatt
     3061 acaagataat ttaggtagat taatagataa agtaactggc ctggatcaat caggcaatga
     3121 ttatggcgat gggacaagtt attaatctca taatacttta aaatcaaaaa agaacaccaa
     3181 tgtcaatata tctatctcgc ctatcattcg gcttttctcg ccgtttacta gttgtcttgc
     3241 aaacggaagc agcagaatgt ggattggcgt gtttggtatc ggtattgggg tttcatggtt
     3301 tttatacgga tttgcgccat ttaagggcgc gtttttcttt gtctcttaaa ggcgcaacat
     3361 tggcagattt ggtacggttt gctaatagca tgaatttaac tgctcgagct gttcggttgg
     3421 atttagatga attggtaaat ttgcgcttac cctgtatttt acattgggat ttaaatcatt
     3481 ttgtggtgtt gcatgaagtt catcgtaatc acattgtgat tatgaaccct gcttcgggtt
     3541 ggcaaaaagt gcgtatggaa gaagtctcgc gctgttttac aggtgtagcg ttggaactat
     3601 ttcctaacac gcaatttgaa gagaaacacg aaacgcgtaa aattcgcgta ctaccgatgt
     3661 tgcgcggagt ggtgggatta aaaaggtctt tatttcagct tctgctgctg gcggcagtat
     3721 tacaggtgtt tgctttggca agcccttttt ttatgcaatg ggtaatagac catgccattg
     3781 tatcggcaga ccgtgattta ttgatgacat tggcattgtg ttttggcttg ttgatgattg
     3841 tgcggcagtt agtgtcattg ttgcaaactt gggcgggtat gtacttatct acctctttga
     3901 atattcaatg gaaagctaat gtattacgcc gcttaatgga tttgcctgtt tcttatttcg
     3961 ctaaacgaca tttgggcgat gtggtttcac gttttggctc agtggacagt attcaaagta
     4021 cgctcacttc tactttcttt gtgttggtgc tcaacagcat tatggcggta tttacattag
     4081 ggttgatgta tttgtatagc ccaagtttaa cgacagttgt actggttgtt ttgctgattt
     4141 atattggtat tcgttgggtg gcatattatc cattacgcca tgcaactcaa gaaaatattg
     4201 ttcatgctgc caagcagagc agttatttca tggaaaccat acgtggtatt caaactgtta
     4261 aattatttga taaaaatgca caacgccatg cggcttggct gaacttgttt attgacacga
     4321 ttaacacagg tttaatgaca caaaaacttt ccgcgctgtt cggttttacg aatggccttt
     4381 tgtttggtat cgctaatatt ttaattgtgt attttggtgc ggtttctgta ttgaacggtg
     4441 gttttaccgt tggggcgtta atggcgttta tgtcctacaa aagtcagttt gagagtaaag
     4501 caggttcatt gattgaccag tttgtacaaa tcaaaatcta ggattacacg cggaacgctt
     4561 ggcggatatt gtgctagaag aaacggaaaa tgaacaaaac aacgatttca gtctgcctaa
     4621 aaatttagaa cattttgata ttcaaataga aaatgtatca tttagctatg ctgaaaatga
     4681 accatacatt ttgcaaaatt tcagtttaat catcaaacaa ggaacagcca ttgcgtttgc
     4741 tggacattca ggctgtggga aatctacgct cattcaaatt ctgacaggca gtctgaaacc
     4801 tgaaagcggt catgtgttat taggtgggca tgacattcat gcgttaccac ctgcatttat
     4861 ccgcacatgg agcgcaagtg tgatgcaaaa tgatgtgtta tttactggct caatcgcaga
     4921 aaacatcagt ttttttgatg atacgcccaa tatggaaaaa atcgcatttt gcgctcaaat
     4981 ggcgaatatt catcatgaaa ttgtcgctat gcctatggcg tatgaaacat tgattggcga
     5041 catggggagt gcattgagtg gtgggcaaaa gcagcgcatc gtactagctc gtgctttata
     5101 tcgtgaacca aaaattttgt ttttagatga agccagtagc catttggaca tcaacaatga
     5161 acgtgtgatt aacgataatc tacgccaact caacattacc aaaatcatgg tcgcgcatcg
     5221 tcaggaaact ttaaatactg ctgatagcgt agtttattta gacaaggtag cctgaaatga
     5281 cattatttcg ccccgaagta ttccaagcta aaaaaaatcg ctggacaggg caaattgttt
     5341 tagtgcgtcc tttttccttg cagtttttaa catttttcgc ggtctcatta gcggctattt
     5401 tagttgcttt tttaattttt gggagctata ccaataaaac caccgtaaca ggacaacttt
     5461 tgccgacaac aggggtagta cgcgtatatt cgcaggatat gggggttatt gctcatcaac
     5521 acgttatgaa tggcgatttt gtgaaaaaag gcgatgtgtt atttacatta tctacttcac
     5581 gaaatgacaa taatggcagc atacaagcac gattactcgc cgaagctgaa ttaaaaaaat
     5641 cactatcaga acaagaaatt attatgaaaa aacgtgttca tgcagcagaa aaaaccgcac
     5701 aagaaaacac ggtacaccgt tttcagaacc aaatgcaaca cgttagaaat caaattatca
     5761 tgcaagaaaa acgtattgcg atttctgaaa aaatgttaga aaaacagcgt tatttagcca
     5821 aaatggacgc aatttctgaa ttagaaaaaa atagttatga aattgcttta ttggagctga
     5881 aagcaggttt agccgcttac caacgagaag cagataatct tgctcgggaa ataactgtac
     5941 agcaaagcaa tctaaaaaat ctgcctgaac agcaagctac tgaaatcagt caattagaac
     6001 gcgcagtatc tgtctatcaa caagaaatat tggattatca acaacgtaac gaacaaacta
     6061 ttcgcgcaac catatcaggc tatgtcagtt ctattaacac ggaaattggt cagcaggtag
     6121 atactaacaa actattgatg agtatcgtgc cgaaagaaag tgaattactg gctaatttat
     6181 atgtccctag tcgcgcaatc ggttttgtaa aaccaaatga caaggtaatt ttgcgttatc
     6241 aagcatatcc ttatcaaaaa ttcggacacg cagaaggaca cgtcatttct attgctcaaa
     6301 cggcactagg ggcgcaagaa tggacaaatt taggcaatat ttttactcaa acagcacaag
     6361 tgaatgagcc tgtttattta atcaaagtaa aactggataa tcagcatatt cgtatatatg
     6421 gcacggagaa gaaattgcaa attggtatga tactagaagc cgatattttg catgagaata
     6481 aacggttata tgaatggata cttgaccctt tgtatcaagt aatgggaaaa atatcatagc
     6541 tgaattgctc aatacattgg agcgtatgag tgcccctaat tatcaagcaa aaccaatgtc
     6601 cgatgacttc caatttgttg ttaagcacaa atacacagtg ttttatgtaa taatgaagac
     6661 agagaagtga tacaattttt cgctccattt ttgcttcctg aattgctgca attgtatctg
     6721 cgttgtatgc gagaagtaaa tattttcgat gcatttgctg actttgtacc ataatacgtc
     6781 ttccaaccag tatgataggc tggtttttct acatgccgat gccgtggccg tcgtcccgct
     6841 ccatacgccg cgcctgccgc tcctgctctt tgcgctggtc ttcccgctcc ttctgctcta
     6901 cttcccttat ccgctgcgcg ccgaaataaa acgcgattcg cttggccgaa gggctgccgt
     6961 tcagggggaa tttttccagt tcgatttgaa ccgccttctg ccctattgtc gtctgtgctt
     7021 cttgggctgc ctgcaaaaat acgttcctga attcttccat cggccgtttg ccgatttccc
     7081 tgcggacatt gtccagttct tcgttaaacc tgtcgcgtac tttgaccacg tcggcggcca
     7141 acttgtcgct atactgccct tccagttttt cgttttctgt tttcagccgc cggatttctc
     7201 gcacgttcgc tccatgccgt tcgttgatgt gttgctgctt ctcgttttcc ttaaccagtt
     7261 ttgcgccgat tttacccatg ccgaaaaagc ggttttcgcc ttggtcttgg actgcccaat
     7321 agtcgtgatg cagccgtttg ttgtcgtctt ccagcgccct tattttgttt cggttttcgt
     7381 tcatgcggta tcgcgcattg atgtaccaat ctccgcgttc tatatcgcgc tccaagccgc
     7441 gcatgtcgtc cagttgtttt tgaaacagcc tgtatatcgt cattagcttg tcgttcggca
     7501 gcttggcgat aaagtgctct tcctgtttcg gcggctcttt cggtgtttcc tgcgcctgct
     7561 ttgcccgctg ctcggcggct tgggcagccc gattataatt aattttatcc cagaattcac
     7621 aatttgagac ctttgcaaaa ttccccaaaa tcccctaaat tcccaccaag acatttaggg
     7681 gatgttccat gagcaccttc ttcca
//
LOCUS       DAXHNE010000095         7663 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.095, whole genome shotgun sequence.
ACCESSION   DAXHNE010000095 DAXHNE010000000
VERSION     DAXHNE010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7663
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.095"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            270..1636
                     /locus_tag="ACFM4Z_001472"
                     /pseudo
     CDS             270..1636
                     /locus_tag="ACFM4Z_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688931.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            1650..1784
                     /locus_tag="ACFM4Z_001473"
     CDS             1650..1784
                     /locus_tag="ACFM4Z_001473"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021492.1"
                     /translation="MPSEGHTSFRQHCLDARHNTRNAEIRTGLPTSVGCFRAGGVPSA
                     "
     gene            1880..2536
                     /gene="cmk"
                     /locus_tag="ACFM4Z_001474"
     CDS             1880..2536
                     /gene="cmk"
                     /locus_tag="ACFM4Z_001474"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFC6021493.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSDSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRASLLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            2691..4376
                     /gene="rpsA"
                     /locus_tag="ACFM4Z_001475"
     CDS             2691..4376
                     /gene="rpsA"
                     /locus_tag="ACFM4Z_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFC6021494.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGNEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            4387..4701
                     /locus_tag="ACFM4Z_001476"
     CDS             4387..4701
                     /locus_tag="ACFM4Z_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFC6021495.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(5052..5459)
                     /locus_tag="ACFM4Z_001477"
     CDS             complement(5052..5459)
                     /locus_tag="ACFM4Z_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFC6021496.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLFLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            5607..6716
                     /locus_tag="ACFM4Z_001478"
     CDS             5607..6716
                     /locus_tag="ACFM4Z_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HFC6021497.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINKAFDLMHEGKSIRAVIHY"
     gene            6725..7552
                     /gene="fghA"
                     /locus_tag="ACFM4Z_001479"
     CDS             6725..7552
                     /gene="fghA"
                     /locus_tag="ACFM4Z_001479"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFC6021498.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVAPDTSPRGEQVPNDDAYDLGQSAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
BASE COUNT         2039 a         1875 c         1834 g         1915 t
ORIGIN      
        1 tcaaattccc tctctttcat cattatttaa atgctatcat taatataatt aacaaaattt
       61 catttccata caccgatgta gaagaataac aaattaatct atttataaac tccgattctt
      121 cctcatcggg ttatactcat cagaaatact cactgaaata tatgcctttt acatggaaaa
      181 tcactagaca gttgaaaagc aatcagtcag ttcctgttaa aaaacgcgtg tagaatttcc
      241 gctttatctc aatcggacac ggtttcaata tgtcttcccc ttcaaatacc aatcgtcaaa
      301 cctggtccag ccgattaacc tatatcctga ccgttgccgg cgcgactgtc ggtttcggcg
      361 cgacgtggcg tttcccgtat ttggtcggtg aaaacggtgg tgcgcgtatg tgtttttatt
      421 ctgtatcgcg atgctggtta tcggcatccc gatgattttg gtggaaaatg tcatcggacg
      481 gcgcaaaggc gtgaacgcgc tggatgcgtt cggcggcccg atgaacggca aacccattgc
      541 caaaatttgg aaactggtcg gctggatggg ctgctcggcg cgttcggcat catggcttat
      601 tacatggtac tcggcggctg ggtaatcagc tatatcgtta atattattgg aggaaatttg
      661 aatatttcca gccccgtcga cggtgtggtt acaaaaggct tctttgccga acacattgaa
      721 acagcccttg ggaaattgcg ttttatacgc tgctttttgt cgccgtgaac caatggattt
      781 tggtcaaagg cgttatcggc ggcattgaaa aagcggcaaa atagctgatg ccgctgctgt
      841 ttttgttcct aatcgcgatg gtcgtccgca acgttaccct tccgggcgca atggaagggg
      901 ttgctttcta tctgaaacct gatttcagca agattaccgc cgaactgttc gtcttcgttt
      961 tggggcaggt attttttgcc ctgagcttgg gtttcggcgt gatgattacc ttgtccagct
     1021 atttggataa aaacgaaaat ctggttcaga cggcagttat cacggcaatt accaatacca
     1081 tcatcgccat acttgcgggc tttatgattt tcccgtcgct cttcagcttc ggcgttgccc
     1141 ccgattccgg cccgactttg gtgttccaaa gcttgccgat tgtgttctca catatgtggg
     1201 cgggatctgt gttcgccgtg attttcttct cgctgctcct gattgccgcg ttgacaactt
     1261 cgctgaccat ttatgaagtg ttgattacga ccattcagga aaaaaccaaa atccgccgta
     1321 ccgccgcgat tacgattgta ttggctgcca tcttcatttt cggcaacatc ccgtccattc
     1381 tgagctatgg tccgtggaaa gacgttccgt gttcggcaaa aatattttcg atgccttcga
     1441 ctacatcagc ggcaacatct tgtttatgct gaccgcgctc ggttccgcgc tgtttgccgg
     1501 ttttgtgatg aaggacgaag cgaaggacga attgctttat aaaggcaacc atacgacggt
     1561 caatatttgg tttgcttatg tgaaatatct tgtgccgctg gtgattctgc tgattttcgt
     1621 cagcaacctg ttctaatccg cagcaatcga tgccgtctga aggtcatacc tctttcagac
     1681 agcattgcct tgatgcccgc cacaataccc gcaacgccga aatccgaacc ggcttgccga
     1741 cttctgtcgg atgtttccgg gcaggcggcg ttccgtctgc ttagacaatc gtcctttaaa
     1801 acaggtagaa tccgccccaa tgggaaatac atccttcaga cggcaaaacc cataccccaa
     1861 accatcagga atccccctta tgaacagaca aaaagtcatc gccatcgacg gcccgggcgc
     1921 atcgggcaaa ggcacggttg ccgcccgcgt tgccgccgca ttgggatacg attatctcga
     1981 taccggcgca ctctaccgcc tgaccgccct atatgcacaa aaacaaggcg tgggatggca
     2041 cgatgaagaa aacgtttccg aactggcaaa aaaactgccc gccgtatttt cagacagccg
     2101 catcctgctc ggcggcgaag acgtttcaga cggcatccgg acagaagcca tcggcatggg
     2161 cgcatccgca gtcgcacagt tgcctaaagt ccgcgcctcc ctgctgcaac gccaacgcga
     2221 ttttctgacc gaaaaaggac tggtcgccga cggacgggac accggatcgg tcgtcttccc
     2281 ccaagccgaa cttaaaatct tcctgacggc agaatccaaa atccgtgccg aacgccgcgc
     2341 caaacaaatc ggcatcccct gcgaaggttt ggcattcgag cgcatcctgt ccgacatcga
     2401 agccagagac gaggcagacc gaaaccgcaa ggttgccccc ctgaaacaac aacccgatgc
     2461 cctgcttttg gatacgagcc gcctgactat agaagaaact gtaaaaaaag tgcttgattg
     2521 gtatcgtaaa gtttaaattt tcaggtataa tcgcccgcat tgcgtttcag acagcatgaa
     2581 tttccccata tgccgtctga aaccttattt acccgtctgc cctgccaagg acggcagaca
     2641 cccaccaacc caccccgcac cccttggcgg tgtaccgaaa agagttatat atgtctatgg
     2701 aaaattttgc tcagctgttg gaagaaagct ttaccctgca agaaatgaac ccgggtgagg
     2761 tgattaccgc tgaagtagtg gcaatcgacc aaaacttcgt taccgtaaac gcaggtctga
     2821 aatcagaatc cctgattgat gtagctgaat tcaaaaacgc tcaaggcgaa attgaagtta
     2881 aagtcggcga cttcgttacc gttaccatcg aatccgtcga aaacggcttc ggcgaaacca
     2941 aactgtcccg cgaaaaagcc aaacgcgctg ccgattggat tgccctggaa gaagccatgg
     3001 aaaacggcaa catcctgtcc ggcatcatca acggcaaagt caaaggcggc ctgaccgtta
     3061 tgattagcag catccgcgca ttcctgccgg gttctttggt cgacgtacgt cctgtaaaag
     3121 acacttctca cttcgaaggc aaagagatcg aattcaaagt gatcaaactg gacaaaaaac
     3181 gcaacaacgt cgttgtttcc cgccgcgccg ttctggaagc cactttgggt gaagaacgca
     3241 aagccctgct ggaaaacctg caagaaggct ccgtcatcaa aggcatcgtt aaaaacatta
     3301 ccgattacgg tgcattcgtt gacttgggcg gcatcgacgg tctgttgcac atcaccgatt
     3361 tggcatggcg gcgcgtgaaa cacccgagcg aagtcttgga agtcggtcag gaagttgaag
     3421 ccaaagtatt gaaattcgac caagaaaaac aacgcgtttc cttgggtatg aaacaactgg
     3481 gcgaagatcc ttggagcggt ctgacccgcc gttatcctca aggcacccgc ctgttcggca
     3541 aagtatccaa cctgaccgac tacggcgcat tcgtcgaaat cgaacaaggc atcgaaggtt
     3601 tggtacacgt ctccgaaatg gactggacca acaaaaacgt acacccgagc aaagtcgtac
     3661 aactgggcga cgaagtcgaa gtcatgattt tggaaatcga cgaaggtcgc cgccgtatct
     3721 ctttgggtat gaaacaatgc caagccaatc cttgggaaga atttgccgcc aaccacaaca
     3781 aaggcgacaa aatctccggc gcggttaaat ccattaccga tttcggcgta ttcgtcggcc
     3841 tgcccggcgg catcgacggt ttggttcacc tgtccgacct gtcctggacc gaatccggcg
     3901 aagaagccgt acgcaaatac aaaaaaggcg aagaagtcga agccgtcgta ttggcaatcg
     3961 acgtggaaaa agaacgcatc tccttgggta tcaaacaact ggaaggcgat ccgttcggca
     4021 acttcatcag cgtgaacgac aaaggttctt tggttaaagg ttccgtgaaa tctgttgacg
     4081 ccaaaggtgc tgttatcgcc ctgtctgacg aagtagaagg ctacctgcct gcttccgaat
     4141 ttgcagccga ccgcgttgaa gatttgacca ccaaactgaa agaaggcaac gaagttgaag
     4201 ccgtcatcgt taccgttgac cgcaaaaacc gcagcatcaa actttccgtt aaagccaaag
     4261 atgccaaaga aagccgcgaa gcactgaact ccgtcaatgc cgccgccaat gcgaatgccg
     4321 gcaccaccag cttgggcgac ctgctgaaag ccaaactctc cggcgaacaa gaataaggtt
     4381 gcagacatga caaagtctga gttaatggtt cgtttggcag aagtgtttgc cgccaaaaac
     4441 ggcacgcatc ttctggcaaa agacgtagag tacagcgtaa aagtcttggt tgacaccatg
     4501 actagatcgc ttgcccgagg tcaacgcatc gaaatccgcg gtttcggcag cttcgatttg
     4561 aaccatcgtc ctgcccgcat cggtcgcaat cccaaaaccg gcgagcgtgt ggaagtacct
     4621 gaaaaacatg taccccactt caagcccggt aaagaattgc gcgagcgggt cgacttggct
     4681 ttaaaagaaa atgccaatta aaccttagca tcaaaacgcc gctgttacgc ggcgtttttt
     4741 ctgtggttta acttcatccg ttgcttcaat accttgagcc aagcaagcaa cggattagag
     4801 cgtggatttt tttatagtgg attaacaaaa accagtacgg cgttgcctcg ccttagctca
     4861 aagagaacaa ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttacac
     4921 tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactatatca ttgcttacaa
     4981 tccgcttttt aaacaacaaa tttttgattt ctattacgaa caggacaaaa atcctgctta
     5041 ttgcactaaa actaagccgt ttcaggaatt tgcggcaaat ttacagcttt taccgagcct
     5101 aatgctttcg ctttttggta aaacgccaat ttgtattcaa gcaaatctaa atagcgtttt
     5161 aattcggcaa tttgacactt cacattttct atttgatttt caaacaagaa aaggcgttct
     5221 tcaatggtat cgtcgccaat gacggtacat tccgcaaagc gtttgatgtc ttttaagctc
     5281 atccccgtat ttttcaagca ttgcaataag cccaaccatt gcaaatcgtt atcggtaaaa
     5341 cagcggttac cgtattcatc acgtccgata ttgggcaaca aaccttcttt gtcgtaaaaa
     5401 cgtagggtgt gggcggagat gcctattttt tcggcggctt tggcagtagt ataagtcatt
     5461 ttccctcctt ctaaacaaaa acagtaaaaa acacttgctt tagagttaac tctaaagtgt
     5521 aaactgttgc tatgttgctc aggcaaggca actttgtcaa tgaattaaga ggaaagacaa
     5581 tggaaatgaa acaagccgat tcaaccatca aatctcgtgc ggcggtggca ttcgccccta
     5641 accaaccctt acaaattgtg gaaatcgacg tagaaatgcc gcgtaaaggc gaggtgttaa
     5701 tccgcaatac ccacactggc gtgtgccata ctgatgcgtt tacgttatca ggaagcgatc
     5761 ctgaaggcgt attccctgtg gtgcttggac acgaaggtgc gggtgtggtc gttgctgtgg
     5821 gcgaaggtgt gtcaagcgta aaaccgggtg atcacgtgat tccgctttac accgccgaat
     5881 gtggcgaatg tgagttttgt tgttcaggta aaaccaactt gtgcgtctca gtgcgtgata
     5941 cacaaggtaa aggcttaatg ccggacggca cgacgcgttt ttcttatcaa ggtcagccga
     6001 tttatcacta tatgggctgt tcgactttca gtgaatactc cgttgttgcc gaagtttcac
     6061 tggcgaaaat caaccctgaa gccaaccatg aacaagtatg tttgctcggc tgcggcgtta
     6121 ccacaggtat tggtgcggta cataatacgg caaaagtgca agaaggcgac tctgttgccg
     6181 tgtttggctt aggcgcgatt ggtttggcgg tggtgcaagg tgcgcgtcaa gccaaagccg
     6241 gccgcattat cgccattgat accaatccat caaaatttga gttggcaaaa cagtttggtg
     6301 caacggattg tttgaacccg aacgattacg ataaaccgat caaagatgtg ttgttagaca
     6361 ttaataaatg gggcattgac catacctttg aatgtatcgg caacgtaaac gtaatgcgtc
     6421 aggcattaga aagtgcacat cgtggttggg gacaatccat tatcatcggc gtagcgggtg
     6481 cgggacaaga aatttcaacg cgtccgttcc aattggtaac aggtcgtgtt tggaaaggct
     6541 ctgcatttgg tggtgtgaaa ggtcgctccg aactgccgaa aatggtggaa gattcaatga
     6601 aaggcgatat cgagttagaa ccgtttgtaa cccacacaat gacactcgat caaatcaata
     6661 aagcctttga cttaatgcac gaaggtaaat cgatccgcgc cgttattcac tactaaggta
     6721 tgcgatgaaa ctgattgaac aacatcaaat ttttggcggt tcgcaacaag tttgggcgca
     6781 tcatgcccaa acgctgcaat gcgaaatgaa atttgccgtc tatttgccaa ataatccaga
     6841 aaatcgaccg cttggtgtga tttattggct ttccggcttg acgtgtacgg aacaaaattt
     6901 cattaccaaa tcaggctttc agcgttatgc ggcagaacat caagtaattg tggtggcccc
     6961 cgataccagc cctcgcggag agcaagtgcc gaacgatgat gcttacgatt taggacagag
     7021 tgcaggcttt tatttgaatg cgaccgaaca gccttgggcg gcgaattatc aaatgtatga
     7081 ttacattttg aacgagctgc cccgtctgat tgagaaacac tttcctacca acggcaaacg
     7141 ttccattatg gggcattcaa tgggcggaca cggcgcattg gtattggcgc tgcggaatca
     7201 ggaacgttat caaagtgttt ctgccttttc gcctatttta tcgccaagcc tcgtgccgtg
     7261 gggagaaaaa gcctttactg cttatttagg gaaagaccgt gaaaaatggc agcaatatga
     7321 tgctaactca ctcattcaac aaggctataa agtgcaaggt atgcgcatcg atcaaggctt
     7381 ggaagatgag tttttgccga cacaattgcg taccgaagat tttatcgaaa cctgccgtgc
     7441 ggcaaaccag ccggtcgatg tgcgtttcca taaaggctac gatcacagct attacttcat
     7501 cgccagtttt attggcgagc atattgctta tcacgccgcg tttttgaagt aaaccaaaga
     7561 gcgttcagtg ttcaaagcag ttttgggata gccggcacga gggcggtaag aagtgccggc
     7621 ataaacgtat gccgtctgag ccggaaggag ccgactctac gga
//
LOCUS       DAXHNE010000096         7549 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.096, whole genome shotgun sequence.
ACCESSION   DAXHNE010000096 DAXHNE010000000
VERSION     DAXHNE010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.096"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            1..420
                     /locus_tag="ACFM4Z_001480"
                     /pseudo
     CDS             1..420
                     /locus_tag="ACFM4Z_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(455..1330)
                     /gene="rsmI"
                     /locus_tag="ACFM4Z_001481"
     CDS             complement(455..1330)
                     /gene="rsmI"
                     /locus_tag="ACFM4Z_001481"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFC6021499.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREAGFKVVPVVGASAVMAALSVAGVEGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            1376..1723
                     /locus_tag="ACFM4Z_001482"
     CDS             1376..1723
                     /locus_tag="ACFM4Z_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFC6021500.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGVAYSISPSKLLKLQRSVEYYLQLNRLTNVPCRLDAVLIE
                     GNRPPEWIQNITG"
     gene            1728..2321
                     /locus_tag="ACFM4Z_001483"
     CDS             1728..2321
                     /locus_tag="ACFM4Z_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HFC6021501.1"
                     /translation="MTTLQERVATHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPYPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            2385..2993
                     /locus_tag="ACFM4Z_001484"
     CDS             2385..2993
                     /locus_tag="ACFM4Z_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFC6021502.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGGAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLHQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3001..3651
                     /locus_tag="ACFM4Z_001485"
     CDS             3001..3651
                     /locus_tag="ACFM4Z_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021503.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRAVLLICI
                     TAIGALAIGIVSTFNPNGDKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPRPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPPKLHKEILDNLF"
     gene            3691..4470
                     /gene="map"
                     /locus_tag="ACFM4Z_001486"
     CDS             3691..4470
                     /gene="map"
                     /locus_tag="ACFM4Z_001486"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFC6021504.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            4625..4924
                     /locus_tag="ACFM4Z_001487"
     CDS             4625..4924
                     /locus_tag="ACFM4Z_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6021505.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5162..5536
                     /locus_tag="ACFM4Z_001488"
     CDS             5162..5536
                     /locus_tag="ACFM4Z_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC6021506.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(5724..7190)
                     /locus_tag="ACFM4Z_001489"
     CDS             complement(5724..7190)
                     /locus_tag="ACFM4Z_001489"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFC6021507.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSTPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         1940 a         2274 c         1849 g         1486 t
ORIGIN      
        1 aagaggcgga atgggtgggc agcctgttcg gcctgcacaa atctttcggt ttccttacgc
       61 tgacggtgat tacattgcgc atcgtgtggg cggttgccaa ccgcgccaag cgtccgcaaa
      121 gcgactgcaa ggctgcggcg gcaggccacg gcattctgta tctgctcatg cttgctgttc
      181 ccgttatcgg catgatccgc caatacggca gcggccgcgg cccgttgaaa gtgttcggcg
      241 ttgaagtgat gcaagggttc gccggaaaaa atcgagtgga tggcaaactt gggcaacacg
      301 ttccacggca atttgggctt gctgctgttt gccgccgtcg ccggacacgt cgccatggtc
      361 gtcgcccacc gtgttcaggg tagagatgtt ctgtgccgca tgacgggtcg tgtccgctga
      421 ttccgttcac actatggtgc cggctcgtcc ggcactattt gtttttccaa gacagagcca
      481 gatcgtacaa agctttcttt ccctcgcccg tgattttggc agcaagctcc gccgcctgtt
      541 tggtcggcag ctcggctgtg aggattttca tgatgttttg cgcggactcg gacaagcctt
      601 cgtgtttttc atcctgcgcc ggataaagca ccaacaccat ctcgccgcgc gattggttgc
      661 cgtcggcaga caatgccgtc tgaatttccc caaccgtgcc gcttaagaac gtttcaaacg
      721 ttttcgtaat ttcgcgcgcc agcattaatc ggcgttcggg gaacagttcc gccatatcgg
      781 caagcgtcgc accgatgcgg tgcggcgttt caaacatgac gataggaaac gccgcccgca
      841 cccatttggc aaacagtttc ctgcgttctc ccgatttcgg cggtacaaaa ccgttgaaat
      901 aaaaatcgga tccttccaca ccggccacgc tcaaagccgc catcaccgcg cttgcgccca
      961 cgacgggaac gactttaaac ccggcctcac gcacgcggcg ggcgagtttc gcgcccgggt
     1021 cgcacacggc cggcgtaccc gcatcggaaa cctgtgccac aaccatgccg tctgaaagat
     1081 agccgacaat cttgtccgcc atctgccgtt cgttgtgttc gcgcacactg acgagtttgc
     1141 cctgaatgcc gtacgcgctc aaaagctgtg cggtaacgcg cgtgtcttcg gcacagatga
     1201 tgtccgcctt ttgcaatacc gccaaagcgc gcagggtaat gtccgccaaa ttgccgatgg
     1261 gcgtggcaac cacgtataat gtccctccga cgacgctgtc ggaggctttc tgcaaatgtt
     1321 tctgaaacat aagaatgccg tctgaaaaac aaacattata aaggttaaac cgattatgcg
     1381 cctaaaccac aaacagggcg aggcagggga agatgccgcg cttgccttcc tccaatccca
     1441 aggctgcacg ctgcttgccc gcaactggca ctgcgcctac ggcgaaatcg acctgattgt
     1501 caaaaacggc ggcatgattc tgtttgttga agtaaaatac cgcaaaaatc ggcaattcgg
     1561 cggtgtcgca tacagcattt ccccatccaa attattgaaa ctgcaacgaa gtgtagagta
     1621 ttatctgcaa ctgaacaggc tgacaaacgt accgtgccgc ctcgatgcgg tacttatcga
     1681 aggaaaccgc ccgcccgagt ggatacagaa tattacaggt tgacgatatg acgacattac
     1741 aagaacgcgt tgccacccat tttgccgaaa gcatccgtgc caagcaggaa gccggaaaag
     1801 tattggtcga gccgaccgta caggctgccg agctgatgct gcaatgcctg atgaacgacg
     1861 gcaagattct ggcgtgcggc aacggcggtt cggccgccga cgcgcagcac ttcgccgccg
     1921 aaatgaccgg gcgttttgaa aaagaacgta tggaactcgc cgccgtcgcc ctgaccacag
     1981 acacctccgc gctgaccgcc atcggcaatg actacggctt cgaccacgtc ttcagcaaac
     2041 aagtacgcgc cctcggacgc gccggcgacg ttctcgtcgg catttccact tccggcaatt
     2101 ccgccaacgt catcgaagcc gtcaaagccg cacacgaacg cgatatgcac gtcatcgccc
     2161 tgaccggccg cgacggcggc aaaatcgccg ccatactcaa agacaccgac gttttactca
     2221 acgttcccta tcctcgaact gcccgtattc aggaaaacca catcctgctg atacacgcca
     2281 tgtgcgactg tatcgactcc gtactgctgg aaggaatgta acccttttca gacggcatgg
     2341 cgcaaagcaa tgccgtctga aacgcccaag aaaggaagcc cccgatgaaa cccaaaccgc
     2401 acaccgtccg caccctgatt gccgccattt tcagccttgc ccttagcggc tgcgtcagcg
     2461 cagtaatcgg cggggcggcg gtcggcgcga aatccgccgt cgaccgccga accaccggcg
     2521 cgcaaaccga cgacaacgtt atggcgttgc gtatcgaaac caccgcccgt tcctacctgc
     2581 accaaaacaa ccaaaccaaa ggctacacgc cccaaatctc cgtcgtcggc tacaaccgcc
     2641 acctgctgct gctcggacaa gtcgccaccg aaggcgagaa acagttcgtc ggtcagattg
     2701 cacgttccga acaggccgcc gaaggcgtgt acaactacat taccgtcgcc tccctgccgc
     2761 gcactgccgg cgacatcgcc ggcgacactt ggaacacatc caaagtccgc gccacgctgt
     2821 tgggcatcag ccccgccaca caggcgcgcg tcaaaatcgt tacctacggc aacgtaacct
     2881 acgttatggg catcctcacc cccgaagaac aggcgcagat tacccaaaaa gtcagcacca
     2941 ccgtcggcgt acaaaaagtc atcaccctct accaaaacta cgtccaacgc tgactcggca
     3001 atgccgtctg aaccgccttc agacggcatt gcccgacacc ccaaaagcac aatcaaaatg
     3061 gcaaaaaaac cgaacaaacc cttcaggctg acccccaaac tcctgatacg cgccgtatta
     3121 ctcatctgta tcaccgccat cggcgcattg gcaataggca tcgtcagcac attcaacccg
     3181 aacggcgaca aaaccctcca aaccgaaccg caacacaccg acagcccccg cgaaaccgaa
     3241 ttctggctgc caaacggcgt agtcggacaa gatgccgccc aacccgaaca ccaccacgcc
     3301 gcctcatccg aacccgcaca gccggacggc acagacgaaa gcggcagcgg actgccgtcc
     3361 cctgccgcac ccaagaaaaa ccgggtcaaa ccgcgccctg ccgacacagc tcaaaccgac
     3421 agacagccgg acgacgccgg aacacaagct gaaaacacac tcaaagaaac ccccgtactg
     3481 cctacaaacg tcccccgtcc cgaacctcga aaagaaacac ccgaaaaaca ggcgcagccc
     3541 aaagaaacac ccaaagaaaa agaaacgccc aaagaaaacc ataccaaacc ggacaccccg
     3601 aaaaacacgc cgcctaaact ccataaagaa attctcgaca acctcttctg acccggcacg
     3661 gcaggcacac ccgcaatcca aggaagcatt atgaacggca tcatcatcaa aacccccgaa
     3721 gaaatcgaaa aaatgcgcga gctgggcaaa ctcgtcgccg aagccctcga ctacatcgga
     3781 caattcgtca aacccggcgt aaccaccgac gaaatcgaca aactcgttta cgactaccac
     3841 gtcaacgtcc aaggcggtta tcccgccccc ctgcactacg gcaacccgcc ctaccccaaa
     3901 tcctgctgca cctccgtcaa ccacgtcatc tgccacggca ttcccgacga caagccgctc
     3961 aaagaaggtg acattatcaa catcgacctc accatcaaaa aagacggctt ccacggcgac
     4021 tccagccgta tgtttaccgt cggcaaagtc tcccccatcg cccaacggct gatcgacgta
     4081 acccacgcct ccatgatggc gggcatagaa gccgtcaaac ccggcgcgac actgggcgac
     4141 gtaggctacg cctgccaaca ggttgccgaa aacgccggct attccgtcgt acaggaattc
     4201 tgcggacacg gcatcgggcg cggtttccac gaagccccgc aagtgttgca ctacggaaaa
     4261 aaagggcagg gccccgttct aaaaccgggt atgattttta ccgtcgaacc gatgatcaac
     4321 caaggcaaac gccacctgcg tatcctcaac gacggctgga cggtggttac caaagaccgc
     4381 tccctctccg cccaatggga acacgaagtt ttggtgaccg aaaccggcta cgaaatcctc
     4441 accgtcagcc ccgcctccgg caaaccctga aaccggacgt atccgcccca taaaaacaaa
     4501 caatgccgtc tgaaagaaac ggcagatatg atatataata taaaaacagg cttgacccgg
     4561 cacattacga aaacaaagca aatcggaatt tgccccgcaa ccagacaaac ttaaaggaag
     4621 ttttatgaaa atatttgaaa atatagaaga tgttaaagcc atccgtaaaa agaccgggct
     4681 gaaccagata gacttctggg gcaaggtcgg cgttacccag tccggaggat cgcgctacga
     4741 aaccggccgc aaaatgccca aacccgtacg cgaactgctc cgcctcgtcc atatcgaatg
     4801 catcgatttg gcgaaagtca acaaaaaaga tatggaaatc gccgccctgt tgaaaaaaca
     4861 ccatcccgac ctgtatgccg aattgtccaa acagaccaag tccgaaagaa aaaaacaaag
     4921 ttaaaccgca acctccggat gcccgacagt ttttcatttc cgaaaaacgc aaacaatgcc
     4981 gtctgaaaca ccggacaggc cgccgtatcc cgcctgccgc ccctgcctca aaccgccgaa
     5041 ccgcccgaac ccgccttttt acaaacttta tccaatttcc tgtttatttc aggatacgcc
     5101 gacattagaa tgtcaaacag ctcgaaacgg gcaaactcca catccatcca aaggaataaa
     5161 aatgaaactt ctgaccaccg caatcctgtc ttccgcaatc gcgctcagca gtatggctgc
     5221 tgctgccggc acgaacaacc ccaccgttgc caaaaaaacc gtcagctacg tctgccagca
     5281 aggtaaaaaa gtcaaagtaa cctacggctt caacaaacag ggtctgacca catacgcttc
     5341 cgccgtcatc aacggcaaac gtgtgcaaat gcctgtcaat ttggataaat ccgacaatgt
     5401 ggaaacattc tacggcaaag aaggcggtta tgttttgggt accggcgtga tggatggcaa
     5461 atcttaccgc aaacagccca ttatgattac cgcacctgac aaccaaatcg tcttcaaaga
     5521 ctgttcccca cgttaatcag gcaacaaaaa acagcgtttt cagaaatgaa aacgctgttt
     5581 ttttgaccgt tccattattc gcaaaaggga aaaaacgatt acctgccccg tgtatcaaaa
     5641 cctgccctgc cggatgaagg gcataaccgg cagggacggc atcaacacca tatgggggta
     5701 cggcttttct tgaaagattc ggcttaaata tccaacactt tcgcggtata ggcgataatt
     5761 tcatccgccc tttcggggtt ttcgttcaac ttgatgccgt aacccggtac cagctctttc
     5821 agacggcctt cccaagacgg ggcgcgctcg gggaagcatt ggtgcatcaa ccggatcatc
     5881 agcggcacag cagtcgatgc gcccggcgac gcgcccagca atgcggcgag tgagccgtcg
     5941 gcgtgggcga caatctccgt accaaactgg agcacgccgc ctttttcgga gtctttttta
     6001 atgatttgga cgcgttgccc tgcggtgatg agttcccagt cgtcggggtt tgcctcgggg
     6061 tagtattcca gcagggaggc gaagcgttct tctttggttt tacgcaattc gcccagcagg
     6121 tatttggtca gcggcatatt cgcccagccg gcgcacagca taggatagag gttgtccata
     6181 tggatggaca gcggcaaatc cataagcgag ccttgcttga ggaagttgga acggaagcct
     6241 gcgtaagggc cgaacataag gtggcgtttg ccgtccacgt tgcgtgtgtc gaggtgcggg
     6301 acggacatcg gcggcgcgcc gacggaagcc tgcccgtaca ctttggcgtt gtgttgttcg
     6361 gcggtttcag gggtgctgtt gcggaagaac aggccggaca cggggaagcc gccgtagcct
     6421 ttgccttcgg ggatgccgga tttttgcagc agggtcagcg cgccgccgcc cgcgccgagg
     6481 aagaggaagc gggtacggag ggtgagctgc ccgtcggggt tgcgggtatc ggcggttttg
     6541 agcacccacg cgccgtcgga ttcgcgtttg atgtcttcga cgtggcggtt gaactcggtt
     6601 tttacgccct tgccctgcaa atatttcacc atttggcgcg tcagccgtcc gaaatcgaca
     6661 tccgtgcctt cggcggagta gttggcggcg acgggttggt tttcgtcccg gccgcgcatc
     6721 atcagcggag cccaatcgga aattttgttc cgatcggtgg aaaattccat attttcaaaa
     6781 agtttttggg ttttaaacgc gtcataacgt ttttgaagat aagaacaatg gtcttcattc
     6841 atcaccaaag acatatgcgg cacggcattg atgaaggaat tgtcttccaa cttgccttcc
     6901 gcgaccaacg tcgcccaaaa ctggcggctg acatgaaact gttcggcaat attgagggcg
     6961 cgcgccggat cgataatccc atttgcaccc aacggcgcat agttcaattc gcacagcgcg
     7021 gaatgccccg tgccggcgtt gttccacgcg tttgacgatt ccaacgccac atcttccaag
     7081 cgttcaatca gggtgatttc ccaagacggt tcgagttctt tgagcaaaac gcccaaagtc
     7141 gcgctcataa tgccgccgcc caccaagaca acgtctgtcg cttcagccat ggtttactcc
     7201 taaaaaacag gcatcttctg cccttatggt tatttgccgt actacaaacg cctgaatcgc
     7261 aaaagcaggg aaaaccggca atggtgtgtg tccgagtatg ctgtttcggg gttggaatgc
     7321 gttgcaagta tggcttccga caccgcttca ggggcttgta atatgttatc gtgaatgtag
     7381 tggattttac tgggaaatgc aaagtttttc tgtcgcccgc caagcccggg aactgcgaaa
     7441 tgaaaaataa aaatagttat ttatctatat atatcaaatt tttaatagat aaaaaaccaa
     7501 aattgtttat atattaattt ttaaaagatt gtcagcatat tgcgttaag
//
LOCUS       DAXHNE010000097         7536 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.097, whole genome shotgun sequence.
ACCESSION   DAXHNE010000097 DAXHNE010000000
VERSION     DAXHNE010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7536
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.097"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            165..326
                     /locus_tag="ACFM4Z_001490"
     CDS             165..326
                     /locus_tag="ACFM4Z_001490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021508.1"
                     /translation="MPAPATIRQIPLFFPNRLQVSPIRRVMPPPPFKGNARVNGFAVG
                     KAAGYRYVS"
     gene            433..957
                     /gene="nspA"
                     /locus_tag="ACFM4Z_001491"
     CDS             433..957
                     /gene="nspA"
                     /locus_tag="ACFM4Z_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HFC6021509.1"
                     /translation="MKKALATLIALAIPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSTGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSAGVRVKF"
     gene            1093..1482
                     /locus_tag="ACFM4Z_001492"
     CDS             1093..1482
                     /locus_tag="ACFM4Z_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC6021510.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVATLPKGVQVEAEAVLVLNA"
     gene            1559..2389
                     /locus_tag="ACFM4Z_001493"
     CDS             1559..2389
                     /locus_tag="ACFM4Z_001493"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HFC6021511.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRIAKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            2404..3861
                     /locus_tag="ACFM4Z_001494"
     CDS             2404..3861
                     /locus_tag="ACFM4Z_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFC6021512.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSYIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(3902..4291)
                     /locus_tag="ACFM4Z_001495"
     CDS             complement(3902..4291)
                     /locus_tag="ACFM4Z_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021513.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLF"
     gene            complement(4473..4874)
                     /locus_tag="ACFM4Z_001496"
     CDS             complement(4473..4874)
                     /locus_tag="ACFM4Z_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021514.1"
                     /translation="MKIKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFDF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(5291..5620)
                     /locus_tag="ACFM4Z_001497"
     CDS             complement(5291..5620)
                     /locus_tag="ACFM4Z_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021515.1"
                     /translation="MTDKSKTEKLISSDDKQSVIDGILDMVFNSKAYEVPWISEKLME
                     LSKNKDLDIAGLSLTCFGHLARLHSNIGDYDKVIPLLHSKQDDPELQGRAEDALEDIS
                     LFLSENH"
     gene            complement(5646..6353)
                     /locus_tag="ACFM4Z_001498"
     CDS             complement(5646..6353)
                     /locus_tag="ACFM4Z_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HFC6021516.1"
                     /translation="MVKTADGYKAIARIRTGDRVFAKDEASGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNNQTLISNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITF
                     KQQPLKAYNLTVADWHTYFVKGSQAETEGVWVHNDCPYDKGNQRYKDASYHGKNDNSV
                     KSRAPTNGQAALDNSVQVKSTSPRRVGVDKANNEIVVLNKTQTFNNGSAEYHGHVRSW
                     QDLHTDQKNALKKAGLD"
     gene            complement(6488..6805)
                     /locus_tag="ACFM4Z_001499"
     CDS             complement(6488..6805)
                     /locus_tag="ACFM4Z_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021517.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            complement(6808..>7536)
                     /gene="mafB"
                     /locus_tag="ACFM4Z_001500"
     CDS             complement(6808..>7536)
                     /gene="mafB"
                     /locus_tag="ACFM4Z_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC6021518.1"
                     /translation="DTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNAKLDRWGNS
                     MEFINGVAAGALNPFISAGEAVDQWMQENPNAAETVEALVNVLPFAKVKNLTKAAKPG
                     KAAVSGDFSDSYKHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKIHDGAQGKHISGHR
                     NYIEGKSTLNQNINPQELLNGIHSGAYPVISKGARGNPVVDFGYPIGSDGKSGLSTNF
                     GTIHSGKNGVHIVPANPKTIKKVQ"
BASE COUNT         1842 a         2074 c         1589 g         2031 t
ORIGIN      
        1 cgcaaacaaa tcggcggccg cccgggaaaa cccgccccga acgcgtccgg aaaatatgct
       61 tatcgatgga aaacgcagcc gcatcccccg ccgggcgttt cagacggcac agccgccgcc
      121 ggaaatgtcc gacgcttaag gcacagacgc acacaaaaaa ccgtatgcct gcacctgcaa
      181 caatccgaca gataccgctg ttttttccaa accgtttgca agtttcaccc atccgccgcg
      241 tgatgccgcc accaccattt aaaggcaacg cgcgggttaa cggctttgcc gtcggcaaag
      301 cagccggata ccgctacgta tcttgaagta ttgaaaatat tgcgatgcaa aaagaaaatt
      361 taagtataat acagcaggat tctttaacgg attcttaaca atttttctaa ctgaccataa
      421 aggaaccaaa atatgaaaaa agcacttgcc acactgattg ccctcgctat cccggccgcc
      481 gcactggcgg aaggcgcatc cggcttttac gtccaagccg atgccgcaca cgcaaaagcc
      541 tcaagctctt taggttctgc caaaggcttc agcccgcgca tctccgcagg ctaccgcatc
      601 aacgacctcc gcttcgccgt cgattacacg cgctacaaaa actataaagc cccatccacc
      661 gatttcaaac tttacagcat cggcgcgtcc gccatttacg acttcgacac ccaatcgccc
      721 gtcaaaccgt atctcggcgc gcgcttgagc ctcaaccgcg cctccgtcga cttgggcggc
      781 agcgacagct tcagccaaac ctccaccggc ctcggcgtat tggcgggcgt aagctatgcc
      841 gttaccccga atgtcgattt ggatgccggc taccgctaca actacatcgg caaagtcaac
      901 actgtcaaaa acgtccgttc cggcgaactg tccgccggcg tgcgcgtcaa attctgatat
      961 gcgccttatt ctgcaaaccg ccgagccttc ggcggttttg ttttctgcca ccgcaactac
     1021 acaagccggc ggttttgtac gataatcccg aatgctgcgg cttctgccgc cctatttttt
     1081 gaggaatccg aaatgtccaa aaccatcatc cacaccgaca aagcccccgc cgccatcggc
     1141 gcatacagcc aagccgtccg agcaggcgac accgtttaca tgagcggtca aattcccctt
     1201 gatcccgcca ccatgactgt tgtcggcaac ggcgacttcc gcgccgaagc gcgccaagtg
     1261 ttccaaaacc tgcaagccgt cgccgaagcg gcaggcggca cgctggccga catcgtcaaa
     1321 ctcaacgcct acctgaccga cttgggcaat ttcgccgtct tcaacgaagt gatggcggaa
     1381 tttattgccg agcccttccc cgcccgcgcc gccgtcggcg ttgccacgct gcccaaaggc
     1441 gtgcaggtcg aagccgaagc cgtcctcgtt ttgaacgcat aattccacgt tcggacggca
     1501 taagatttcc aaccgtgccg tccgaaccgc aacactttcc cacttcacaa catctgcaat
     1561 ggcacattac gcaatcggcg acatacaagg ctgtttcgac gaactgaccg cgctgctcgg
     1621 caaaatcggt ttcaaccacg gcacggacac cctctggctg acgggcgaca tcgtcaaccg
     1681 cggcccgaaa tccctcgaaa cgctgcaatt ctgcatccgg cacgaaaaca gcgtgcaaat
     1741 cgtcctcggc aaccacgacc tgcacctgct cgctgtcggc tgcggcgaag gcgcgcccaa
     1801 acgcagcgac acaatcgaac ccatactcaa acaccccgac ggaaaaaaaa tgctcgactg
     1861 gctgcgtgcg caaccgctgt tgatacgcga gggcagccgc gtgatggtac acgccggcat
     1921 cctgccgcaa tggcgcatcg ccaaagccga atcgctcgct agagaagccg aagccgaact
     1981 gcgcggcaaa aaatacgtca aattcttctc caaaatgtac ggcaacaaac cggccgcgtg
     2041 ggacgaaggt ttggaaggct atgcccgcct gcgcttcatc gtcaacgcct tcacgcggat
     2101 gcgcgccctg acctttaaaa acgaactgga tttcgactac aaatccacag tgaaaaaaat
     2161 gccgccttac ctgcgcccgt ggttcaaagc ccccgaccgg caaaacctcg accgcaccat
     2221 catcttcgga cactggtcct cgctgggcta cacgaatgcc gacaacgtca tctcgctgga
     2281 caccggcgcg ctgtggggcg gacagctgac cgccgtcaac ctcgaaacgg aagaaattac
     2341 ccaagtccaa gccgccggcg gcatagactg gaaaagcttc gcaaaataac ggaccgcccc
     2401 actatgcaca aaatcctgcc catcgcccac gtcctctccc gactgggtat gctgttttcc
     2461 tttatcctgc tgatacccgc cgccctctcc tacgcctttt cggacggcgc gtacaccgcc
     2521 ttcgccacca ccgcgaccgt taccctttcc ggctcgtaca tcgtccggct cgccaccctc
     2581 cggttcaggc gcgaactgcg cccgcgcgac ggcttcaccc tcgtcctgat gttgtggctg
     2641 gcgtttgccg ctatggcggc gatgccgatg tacctgtatt tcccgaatat gggctttacc
     2701 gacgcatttt ttgaatcgat gtcgggactg accaccaccg gcgcgaccgt catcccccac
     2761 gtcgacgggc tcgccccctc cgtcaacttt tggcggcata tgctcaactg gctgggcggg
     2821 atgggcatca tcgtccttgc cgtcgccatc ctgcctatgc tcggcgtagg cggcacgcag
     2881 ttattcaaag ccgaaatccc cggcattgac aaagaaagca aaatgtcgcc gcgcatttcc
     2941 caagtggcga aaaaactttg gttcggctac accctgatca ccatcctcgc ggcagcctgc
     3001 ctgcattttg ccgggatggg ctggttcgat gccgtctgtc acgcgatggc gaccctctcg
     3061 ctgggcgggt tttccaccca cgatgccagc atagcttatt acaactcccc cctcatcgaa
     3121 gcggtcatca tcgttttcac cattgtcggc ggcatcaatt ttgccaacca tttcgccgcc
     3181 ctcaacagcc gcagcctcaa aacctattgg aaagacgaag aatgccggac gatgctgctg
     3241 ctgctctccg gcagcatcct tgccgccgcc ctgtacctgt ggcacaccgg ctattacgcc
     3301 ggttttaccg aatccctgcg ctacaccgcc ttcaacttcg tctccatcgg attggcaaac
     3361 gggctggcca acaccgactt cgcacaatgg ccgctcctga tttccctgtg gatgtttttc
     3421 ctcgccaaca tcctcgccaa ctccggctcg accggcggcg gcatcaaaac catacgcgcc
     3481 ctcgtcctgt tcaaattcag cctgcgcgaa atgatggtgc tgctgcaccc caaagccgtc
     3541 cgcaccgtca aaatcagcgg caaggccata cccgaccgcc tcgcgctgac cgttatgtcc
     3601 ttcatcttca tctactttat gaccgtcgtc ctcttcagct tcctgctgat ggcgagcggt
     3661 atggaattta ccaccgcctt caccgccgtc atcgcctgca tcaccaacgc cggccccggc
     3721 ctgggcgaag tcgggcccgc cggcaattac gccggtttgg acgtgatgca gaaatggatc
     3781 tgcgttaccg ccatgctctt gggcaggctg gaaatcttca ccgtcttcat cctcttcacc
     3841 cccgcctact ggaagaaata aaaaatgccg tctgaaaact tttcagacgg catattgtct
     3901 tttaaaaaag cggaagctct acaaaaggaa agaaatgctg aaacctctga taaatttcag
     3961 gatatttctc taaggctttt aatgcttttt cttttgaaat aggcggatca tagacatcta
     4021 tcccccttaa gaaggcaatg cccgttaatg catttttttg atcatttgaa atgtaaccat
     4081 aattaacgac tccaattaca tcataaattt cccctatact cttatcaaaa tcaatcaaat
     4141 caaaaatata caatggaatt gtatcaatag gagtatctct gacaactata tccaaccata
     4201 atttaaattc agaaagatta atagcatcat agaacataca gactattgca aatcctaaat
     4261 cttcacaatt ttctttacta attttccaca taattattta cctttatcca ttgcttccca
     4321 accgatacat ccggtatttt agcttctcct tttccgtcaa acactccttt ggttacctgc
     4381 tttgtgtcaa aaataccgtc tgaaagccga atattgtttc agacggcatt ttgattgttg
     4441 ggtttttaat cagagctttg tttgataatt tgttattttg aataaaatac aggttctgtc
     4501 gagctgataa cttctattac agaatcgcta aaaaagaaaa tataagcctt ataatatttc
     4561 ttttcaaaaa aatcaaaact ttcttctttc aaccagtcca tataactgtc atctattatc
     4621 tcgaaaaaat aatttatagc aggatttcta tcccaatcat ttttttcttt attttcaaat
     4681 aaatattctg aaaattttac tttataagat tcatctgtta tttgaaaatg aattatattg
     4741 tcaataaata tttcaatatt taattcataa tttcggtcgg atgccaaaat gttcacatct
     4801 tgttcggtaa cacaaatttt gtctacgtaa aatctgtgat tattgttaga aaattgtaat
     4861 ggtttaatct tcactatcgt tttccatatc tgatttttgt tttcttattg ccctgccgga
     4921 taaagcaggt ctgcctccgt actgtctatc ctcacattta ctttcctgcc atcggctttt
     4981 atctccccag ttttaccgtt tttgccaact tcgcggcttt ggcggcggca atgttgaagg
     5041 cggcttcgac ggtttcggcg gcattggggt tgacccgtac tcatccgcaa acggcaacac
     5101 gacggacggt gtttgctttt cagccgtctg ccgttttgac caaggtgctg ccgtggaagg
     5161 agcaagtgta ggatttttga agaactcagt tctcaatatc ctatttcaaa tgaaagaaat
     5221 gccgtctgaa aattaaacac ggtttcagac ggcattttga ttgttgggtt ttcaacccga
     5281 cctacggttc ctaatgattt tcagataaaa ataaagaaat atcttctaac gcatcttcag
     5341 ccctaccttg aagctctgga tcatcttgct ttgaatgtag taaaggaata actttatcgt
     5401 aatcaccgat atttgaatgt agcctggcga gatgtccgaa acaggttagc gataatccgg
     5461 caatatccaa gtctttattt ttcgataatt ccatcaattt ctcagaaatc cacggtactt
     5521 catatgcttt ggaattaaat accatatcaa gaatgccatc tataacactt tgtttatcat
     5581 cagaagaaat caacttttct gttttacttt tatcagtcat tttttaattt ttccttttga
     5641 attaactaat ccaatcctgc tttttttaaa gcatttttct gatcggtatg caaatcttgc
     5701 caacttctga catgcccgtg atattccgca gaaccgttat taaaagtttg agttttgttt
     5761 aatacaacga tttcattatt ggctttatca accccaactc ttcgaggaga agttgattta
     5821 acttgaacgg aattatcaag agctgcttgt ccgtttgttg gtgctctact tttcacagaa
     5881 ttatcatttt tgccatgata agaagcgtct ttatatcgtt ggttgccttt atcatacgga
     5941 caatcattat gaacccaaac cccttccgtt tccgcctgac tgcccttgac gaagtaggta
     6001 tgccaatcgg cgacggtcag attgtaggct ttgagcggct gctgtttgaa ggtaatgttt
     6061 tgaaccgtct gttttgcgcc gctttcggaa agcagggtgt cgcctttttt cagacgacct
     6121 gcctgtatcc attttccttg actgtaaaac gggtggattt tattggaaat cagggtttgg
     6181 ttgttgccga tgccgtctga aatttcaatg taaacggttt cttgatacgg attgccgtat
     6241 cgggcggtaa cgggtttgta tcccgttttt ccgcttgcct cgtccttggc gaagacgcgg
     6301 tcgccggttc ggatacgggc aatggctttg tagccgtctg ccgttttgac caaggtgctg
     6361 ccgtggaagg agcaggtgta ggatttttga agaactcagt tctcaatatc ctatttcaaa
     6421 tgaaggaaat gccgtctgaa aattaaacac ggtttcagaa gacattgtga ttgttggttt
     6481 ttttaaccta cgcttgcgaa attaattcct ttctcctgaa tactgcacca tttatttggt
     6541 ttaactccat agaagaatga ttagaataaa tacattcaaa gtcagcttca tcaaaatatt
     6601 ctccacacat agaccataac tctgtaatca aatattccat atcttcatgg tcttctgtga
     6661 tggcttctct tgataaataa tttttgattt ttaaaaacct tcttccttcc tcgtaatcta
     6721 cggtaattaa cctgatatct gagtgtatca tgcccagcat tgctatattc ataccgactc
     6781 gcagccagct tggtaatata ttcataacta ttgcaccttt ttaatggttt tagggttagc
     6841 cggaacaatg tgaactccat ttttacctga atgaatcgta ccaaaattgg tacttaatcc
     6901 tgatttccca tcgctgccta taggataccc aaaatcaaca acaggatttc ctcttgctcc
     6961 tttagaaata actggataag cacctgaatg tattccgttc aacaattctt gaggattaat
     7021 gttttgattt aaagtactct taccttcaat gtagtttcta tgtcctgaaa tatgtttccc
     7081 ttgagctcca tcatgaattt tagtcccaat agattttgct ttaaaatcaa cattgcgggt
     7141 ttgaaacatt tctccatcta cagactgaga taatcttgaa gcagtgttat gcttgtagga
     7201 gtctgagaaa tccccactaa ccgcagcctt ccccggtttt gccgcctttg tcaggttttt
     7261 gactttggca aacggcagga cgttgaccag ggcttcgacg gtttcggcgg cattggggtt
     7321 ttcctgcatc cactggtcaa cggcttcgcc cgcgctgata aaggggttga gcgcgccggc
     7381 ggcgacgccg ttgataaact ccatgctgtt gccccagcgg tcgagcttgg cattgtgctc
     7441 gaacattttt ctgttggctt catcggcgcg gtcggagaaa ttgctgccga ggttgttgta
     7501 gttgtcggat atgcgttgcc ggatgctgcg ggtgtc
//
LOCUS       DAXHNE010000098         7306 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.098, whole genome shotgun sequence.
ACCESSION   DAXHNE010000098 DAXHNE010000000
VERSION     DAXHNE010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7306
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.098"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(146..2749)
                     /gene="pepN"
                     /locus_tag="ACFM4Z_001501"
     CDS             complement(146..2749)
                     /gene="pepN"
                     /locus_tag="ACFM4Z_001501"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFC6021519.1"
                     /translation="MSKTVHYLKDYQTPAYHILKTDLHFDINEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGAAADYVLEGETLTIAGVPSERFTVEVETEILPVENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEYSGGRHWVKWEDPFAKPSYLFALVAGDLAVTEDYFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAESRL
                     KNNIFELTIKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAMAFDYQGKRATEAVLLLT
                     EAEQIFLLEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGA
                     ENIDPLRYHQAREALLDILAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYAEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(2880..3674)
                     /locus_tag="ACFM4Z_001502"
     CDS             complement(2880..3674)
                     /locus_tag="ACFM4Z_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFC6021520.1"
                     /translation="MTTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHTGRLKEIRNYCETDAANTYL
                     MYLRFCLVSGRLDADEYEMEIKRMRNYLSAQAGEKPHWEEFVRAWE"
     gene            complement(3788..3922)
                     /locus_tag="ACFM4Z_001503"
     CDS             complement(3788..3922)
                     /locus_tag="ACFM4Z_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021521.1"
                     /translation="MPSERIFRSVLRLVGVSSETVKRKFAGVELSYAGKAAGESLYCL
                     "
     gene            complement(4421..5290)
                     /locus_tag="ACFM4Z_001504"
     CDS             complement(4421..5290)
                     /locus_tag="ACFM4Z_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HFC6021522.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIADLTGLLAYLLVKPRRRIGEIN
                     LAKCFSEWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDIPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLHFYPAWKSFPGEDAKADAQRMNRFIEDRVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(5341..5877)
                     /gene="ruvC"
                     /locus_tag="ACFM4Z_001505"
     CDS             complement(5341..5877)
                     /gene="ruvC"
                     /locus_tag="ACFM4Z_001505"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFC6021523.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPPDAP
                     LADRIAVIVRHIGEVVTVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQPDAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(5880..6119)
                     /locus_tag="ACFM4Z_001506"
     CDS             complement(5880..6119)
                     /locus_tag="ACFM4Z_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HFC6021524.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6150..7160)
                     /gene="dusB"
                     /locus_tag="ACFM4Z_001507"
     CDS             complement(6150..7160)
                     /gene="dusB"
                     /locus_tag="ACFM4Z_001507"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFC6021525.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFVDEGGIVAVQIAGSNPQQMADAARYNVSLGAQVIDINMGCPA
                     KKVCNVQAGSALMQDEPLVAAILEAVVSAAGVPVTLKTRLGWHDDHKNLPVVARIAED
                     CGIAALAVHGRTRTQMYKGEAAYDLIAETKCRLNIPVWVNGDITSPQKAAAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECSAAILNHVRAMHAFYGETAGV
                     RIARKHIGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAGKTDRWACAYRQN
                     AV"
BASE COUNT         1628 a         1937 c         1918 g         1823 t
ORIGIN      
        1 gtcgccttgt cctgattttt gttaatccac tataattaat atgactttgc ggccgttttg
       61 ccattgcgta ataaaacgat ggggaagtga tgataaaacg tgtgtgtaac tatatcagac
      121 ggcattgttt ttctgtttga cggcctcaat ccaaaatttt gccgacgatt tcgcccacgt
      181 ctttcgacaa tccttcctgc gcccgaatgc gctgcaatgc ttgtttcacc aagtttttgc
      241 ggtgcggctc gagcttgttg cagaggttga acgcctgcac caggcgggcg gcgacctgcg
      301 ggttaaagcg gtcgatttcg atgactttgt cggcgatgaa gcggtagccg ctgccgtctt
      361 ctgcgtggaa atgcgggacg ttgcggctga agctgccgat gagcgagcgg gctttgttgg
      421 gattttcgag gctgaacttc ggatgctgca aggcggtttg aacctgttgc agggtgtcgc
      481 tgcggcggct tgagccgacg agggcgaaat atttgtccat caccagcgcg tcgtctgaaa
      541 acttgtcggc aaactgcgcc agcaggcggt tgcgcgtatc gctttcgttg ccgttgacgg
      601 cggacaggat gccccattcg tgggtcatgt tttgcgccat ttcggcgtat ttctcggcaa
      661 cggtttcgat gtgcgcggga tcggcgcgca ggacgaaggc gcggcagaca ttgcgcagcg
      721 tgcgccaacc ggcggcttcg gggctgtact cgtagctttg gttttcctgc ttcgccgcct
      781 gacggttcaa ttcgtgccat ttcggcagaa agcggacggc aagtatatcc aacaaggctt
      841 cgcgcgcctg atggtagcgc agcgggtcga tgttttccgc gccgtcccac agctcggctt
      901 cagacggcac acccaaaagc agggctttga aagcgttgtc taagaggtcg tctgaaatga
      961 ctttttcgac ggcggcaagc agtttttcgt gtttcggcaa cccgatgccg tctgaaagcg
     1021 cggcaaggtt ggcggcgacg gcacggcggt agagcgtttg ggcggcttcc cagcgcgtga
     1081 aggcgtcgct gtcatgggcg agcagaagca gcaggtcgtc gtcgctgtac ggatagttca
     1141 gatgcaccgg cgcgctgaag ccgcgcagca gcgagggaac gacggcttcg gttacgcctt
     1201 cgagcaggaa gatctgttcg gcttcggtca gcagcaatac ggcttcggtc gcgcgtttgc
     1261 cctgataatc gaatgccatc gcttcgccgt tgcggttcaa cagtccgact ttgacgggaa
     1321 tcatcatcgg ctgcttgtcc gccatatcgg gcgtgggcgg cacggtttgt ttgatggtca
     1381 actcgaaaat attgtttttc agacggcttt ccgcttccaa aacgggcgtg cccgcctggc
     1441 tgtaccacaa ggcaaactgg tcgagattga tgccgttcgc gtccgccatc gccgcgcgga
     1501 aatcgtcgca ggtaacagcc tgtccgtcgt ggcgttggaa atagagcttc ataccttttt
     1561 ggaagccctc ttcgccgagc aaggtgtgat acatccgcac cacttccgcg cctttttcat
     1621 aaacggtcat ggtgtaaaaa ttgttcatct cctcatagct ggcggggcgc accggatgtg
     1681 cggtcggacc tgcgtcttca gggaactggt gctggcgcag caggcggatg ttttcgatac
     1741 ggcgcacggc gcggctggcg cggtcgccgg aaaattcttg gtcgcggaac acggtcaacc
     1801 cttccttcag cgaaagctgg aaccagtcgc ggcaggttac gcggttgccc gtccagttgt
     1861 ggaaatattc gtgtccgacc acggattcga tgccttcgaa atcggtatcg gtggcggtac
     1921 ggctgtcggc aaggacgaac ttggtattaa aaatgttcag ccccttgttt tccatcgcgc
     1981 ccatattgaa atcgcccacg gcgacgacca tgaaaatatc caagtcgtat tccaaaccga
     2041 agcgcgtttc gtcccacttc attgcgtttt tcagcgattc cacggcaaag ccgaccttgg
     2101 gcttgtccgc ttcggtggtg taaaactcga ttttgacgtt tctgccgctc atggtggtga
     2161 aatagtcttc cgttaccgcc aaatcgcccg cgaccaaagc aaacagatag ctcggtttgg
     2221 caaacgggtc ttcccatttc acccaatggc ggccgcctga atactcgccg ccgtcgattt
     2281 tgttgccgtt ggaaagcaaa acgggatagc gttttttgtc cgcaacgatg gtggttgtga
     2341 atttggacat cacatccgga cggtcgatgt agaacgtgat tttgcggaag ccctccggct
     2401 cgcactgggt aaatagattg ccgccggaag catacagccc catcagcgat ttgttttcca
     2461 ccggcaggat ttcggtttcc acttcaacgg tgaagcgttc ggatggcacg cctgcaatcg
     2521 tcagcgtctc gccttccaac acataatccg ccgccgcccc gttaattttg acggacaaaa
     2581 gtttcgccga accgtccaac accagcggct cccctaccct ctgcggctca accgtcaaac
     2641 gcgacttcac gacggtttgc ggttcattaa tatcaaaatg taaatcggtt ttgagaatat
     2701 ggtaggcggg cgtttgatag tctttgagat aatgcacggt tttgctcatt tttttctttc
     2761 aatgttattt tgtttgactg gaaaaggctt cagacggcac gggcgcatcc cgcgtatgcc
     2821 gtctgaagcc gcagcggcgg cacgggcgcg ccgccggaca accggtttga attcaatctt
     2881 tattcccacg cgcggacaaa ctcttcccaa tgcggctttt ccccggcttg tgcggacagg
     2941 taattccgca tccgtttgat ttccatttcg tattcgtccg catccagcct gccgctgacc
     3001 agacagaaac gcaggtacat cagataagtg tttgccgcgt cggtttcgca ataattgcgg
     3061 atttccttca gcctgcccgt atggaatgcc tcccaaacct tgctgccgtc catacccagc
     3121 ttgcccggaa aaccgcacag tttcgccata tcgtccagcg gcacgtttgc cctcggctgg
     3181 taaagcgcga gcaaatccat caaatcgcag tggcgttggt gataacggct gatgtagttg
     3241 ttccacttga aatcgcggct gtcgccgaaa tcgccgtcgc ccatatccca atagcgcgcg
     3301 gcgttgatgc cgtatatcag ggagcggtaa tgcagtacgg gcagatcgaa accgccgccg
     3361 ttccaactga ccagttgcgg cgtatgtttt tcaatcaatt cgaaaaattt agcaatgacc
     3421 acttcctcgc cgtcatccat ctcgccgatg gtgccgacat gtactttatc ctgcccccaa
     3481 cgcatgcagc acgaaatcgc cacaacctga tgaagatgat gctgcataaa atcgccgccc
     3541 gtctgagcac ggcgtttttg ctgggcaaac agcaccactt catcgtcggg cagcgaggac
     3601 ggcagctcgt acaatgttcg gataccctgc acatcgggta cggtttcaat atcgaaagcc
     3661 aaaatcgtgg tcatgacagc accttgtatt taaaacggat gcacctattg tgtcattaaa
     3721 aggcggataa aaaaagaggc acccccccac aggattgccc caatacctca aatcagagat
     3781 ttacgcttca caaacaatac aggctttcgc ctgcggcttt acccgcgtag ctcaactcta
     3841 cgccggcaaa ctttcgtttc accgtttccg atgaaacccc gaccaatcgc aagactgacc
     3901 ggaaaatcct ttcagacggc atttcctgcc tgtcgtgtaa ttccatgtag cgaaatgtac
     3961 gccattttct acgctttgcc aagcattttt tacaatataa atgtcaaaac attaatttta
     4021 taaaattgct gaaaatatta aatatatgga tttttatttt ttatatttca ataaatataa
     4081 atttaatttt gatttatatt taaatttaag cataaaatgt caaatattaa agtaaacatg
     4141 aaaggcatat attaaatatt tatttataac gctatgtttt taaagaaaat taattttaat
     4201 atattaacta gattgtctgc atatattcat aggtttgcgg tatttcttcc aaaacctgct
     4261 tcgaatttcc cgaccaagtc ttaaaaatat tgtttttgag atacttaaat agcagcgatt
     4321 atcaaatgaa atctgttcat ataatctgcc attttgcatt taaaaaaaca atcaggagtt
     4381 tcgactcgaa acgcctgata tgttttgtaa ttttacgtag tcagtaaaaa tcggggctgc
     4441 cttccggacg ggttttaaaa cgcttgtgca gccaaaaata ttgttccgga tgttcgcgca
     4501 ccctgtcttc gataaaacgg ttcatgcgct gcgcgtcggc tttcgcgtct tcacccggaa
     4561 aggatttcca agcagggtag aaatgcaatg taaccgtatt gtctgcctcg cggacgggaa
     4621 tggcgggtat cacttttgca tttgcaagcg cggcaatgcg gctcaatccg gtaatcgttg
     4681 ccgtctgaat accgaaaaaa tccacaaaaa ccgaatcgtt gcgtccgaaa tcctgatcgg
     4741 gcagatacag aaacggcgcg ctgcttttgc ggaactgttt gacgagggcg cgcagccctt
     4801 cggtgcgccc gataaggaag acgttgtgat agcggttgcg gcctttcaaa atctgttcgt
     4861 ccaatatctt gtttttttga tgggaataca tactgatcag cgggatatcc tgattaagcg
     4921 cgtacaccgc catctcgaac gcggtaaagt gcggatacag gatgatgact ttttcccccg
     4981 ccgccagcgc gtcgtccaaa taatgcttat tgcggtagcg caccagcgat ttcaaacgtc
     5041 cggcaggcgc gtaccaatat aaaccgtatt ccaacatcag tttcgccatg tgtttgaaat
     5101 gctgtttcaa cacggtttta cgcttttcct cactccattc ggaaaaacat tttgccaaat
     5161 tgatttcgcc gatacggcgg cgcggtttga ccagaaggta ggcaagcaaa cccgtcaggt
     5221 cggcaatctt gtgcagcagc gcaaacggca gaaactgcaa aacatacagt acaaaaaata
     5281 taaatttcat ctcgatacac attttctttt cagacggcaa aatacaaatg ccgtctgaaa
     5341 ctattgaaac ctgccgcgct tgacctgcat ccccgaagga ttgagtttgg cggcaagccc
     5401 gtggttgcgt aaggcgtggg tcagcgcgac ggcaagaccg tccgccgcat ccggctgggg
     5461 cgttcccgaa agtcccaaca tctgcaccac catatgctgc acctgttctt ttgccgcctt
     5521 gcccttgccg actaccgcct gtttgacctg caaggccgtg tattccgaaa cgggcagctt
     5581 atggctgacc aatgccgcca atgccgcgcc cctagcctga ccgagcatca gcgtcgatgc
     5641 cggattgacg ttgacgaaca cctgttccac tgccgcctgt tgaggcttgt aaacggtaac
     5701 gacttcgccg atgtgccgga cgatgacggc aatcctgtct gccagaggcg catcgggagg
     5761 cgttttgatg cagccggagg cgacgtaaaa atgatcgcgc cccctgacat cgatgatgcc
     5821 gaaacccgtt acgcggctgc ccgggtcaat gcccaagata cggacggttg ccgacatatt
     5881 cacaacaaac cgtgttgaat cagtttttta cgcaaggtat tgcggttcag ccccagcatc
     5941 acggaggctt tggactggtt gccgccgcat tgctccatca cgcacaccag cagcggtttt
     6001 tccacctgat gcaagaccat atcgtacacg ccgcaaggtt cggtaccgtt caggtctttg
     6061 aaatattgtt ccaaattttg tctgatacat tgggaaatat cgggaagggt atggggcata
     6121 atcgcgcttt caaaaggata atcgggtgtt cagacggcat tttggcggta ggcgcacgcc
     6181 caacggtcgg tttttccggc aagtctttca agataacctg caagcatgtc gtattgcgcc
     6241 gccgcactgt ccaaacggtt gatttcacga cgtgtctgtt cgccgtcggg catttcgtcg
     6301 atgtaccagc ctatgtgttt gcgtgcaatg cgcacaccgg cggtctcacc ataaaacgcg
     6361 tgcatggcgc ggacgtggtt caaaatggcg gcactgcatt ctgccaaact caaggcaggc
     6421 ggtaaaacac cgtgttcggc gtaatgtttc aaatcgcgga agaaccacgg tctgccttgc
     6481 gcgcctcgcc ctatcatgat gccgtcggcg gcggtttgtt tgaggacggc ggcggctttt
     6541 tgcggcgaag taatgtcgcc gttgacccaa accgggatgt tcagacggca tttggtttcg
     6601 gcaatcaggt cgtaagccgc ttcgcctttg tacatttgcg tgcgcgtgcg tccgtggacg
     6661 gcaagggcgg caatgccgca atcttcggcg atgcgggcga cgacaggcag gtttttgtgg
     6721 tcgtcgtgcc aacccaaacg ggttttgaga gtaacgggta cgcctgccgc actgacgacg
     6781 gcttccaaaa tggcggcaac cagcggctcg tcctgcatca gcgcgctacc ggcttggaca
     6841 ttgcagactt ttttagcggg acagcccatg ttgatgtcga tgacctgcgc tccgaggctg
     6901 acgttataac gcgcggcatc cgccatctgc tgcggattgc tgccggcaat ctgcacggca
     6961 acgatgccgc cttcatcgac aaaatcactg cggtgcaagg tttttctagt atttctgagc
     7021 gtcgggtcgc tggtcagcat ttcgcacacc gcccaacccg cgccgaaatc gcggcagagg
     7081 cggcggaagg gtttgtcggt aatgcccgcc atcggtgcaa gtgcgatggg gttgtcgata
     7141 aaatagccgc cgatgtgcat aatgggcccg cgtttcaaaa aagtacgcca ttgtacattt
     7201 tttaagcagg atttccaatc tccggacgcg cccgcgattg ggtcggacac cgttttatgg
     7261 cataatccgc acacagattc cctgccccgc cactcacagg cgggca
//
LOCUS       DAXHNE010000099         7202 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.099, whole genome shotgun sequence.
ACCESSION   DAXHNE010000099 DAXHNE010000000
VERSION     DAXHNE010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.099"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(62..146)
                     /locus_tag="ACFM4Z_001508"
     tRNA            complement(62..146)
                     /locus_tag="ACFM4Z_001508"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(110..112),aa:Leu,seq:caa)
     gene            422..1075
                     /locus_tag="ACFM4Z_001509"
     CDS             422..1075
                     /locus_tag="ACFM4Z_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HFC6021526.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            1154..1807
                     /gene="hisG"
                     /locus_tag="ACFM4Z_001510"
     CDS             1154..1807
                     /gene="hisG"
                     /locus_tag="ACFM4Z_001510"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFC6021527.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            1911..2810
                     /locus_tag="ACFM4Z_001511"
     CDS             1911..2810
                     /locus_tag="ACFM4Z_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFC6021528.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLDEINSNELILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            2807..4096
                     /gene="hisD"
                     /locus_tag="ACFM4Z_001512"
     CDS             2807..4096
                     /gene="hisD"
                     /locus_tag="ACFM4Z_001512"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFC6021529.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            4142..5239
                     /gene="hisC"
                     /locus_tag="ACFM4Z_001513"
     CDS             4142..5239
                     /gene="hisC"
                     /locus_tag="ACFM4Z_001513"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFC6021530.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGRIPNLIVLRTL
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALRHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            5236..6153
                     /gene="hisB"
                     /locus_tag="ACFM4Z_001514"
     CDS             5236..6153
                     /gene="hisB"
                     /locus_tag="ACFM4Z_001514"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFC6021531.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            6248..7117
                     /locus_tag="ACFM4Z_001515"
     CDS             6248..7117
                     /locus_tag="ACFM4Z_001515"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC6021532.1"
                     /translation="MSANEYAQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
BASE COUNT         1881 a         2118 c         1724 g         1479 t
ORIGIN      
        1 tagtcttatc tatttcatta aacagcaata gacaaaaaaa taaccgctcc aaaagcggtt
       61 gtggtgccca gggtcggact cgaaccgaca caccttgcgg cgggggattt tgagtcccct
      121 gcgtctacca atttcgccac ctgggctggt gaagaagtcg tcattataat ggcttttgaa
      181 attctgtaaa cctttttttg aaattatttt atctgttttt attttatttt tgattttaaa
      241 tagaattttt attattttaa tcttactgtt ctttccgttc caaagattct gtatgattcg
      301 gcaattcctg ccgtgcagac aacgtaaaaa aatactacat taaatctgcc aaacgcgtta
      361 agatggaaat attcaaattc cgtacgaatc aggttttgct atttattctt gggagattgt
      421 catgttttcc gtaccgcgtt cctttttgcc gggcgttttc gtacttgccg cgcttgccgc
      481 ctgcaaacct caagacaaca gtgcggcgca agccgcttct tcaagtgcat ccgcgtcggc
      541 tgcggaaaat gcggcaaagc cgcaaacgcg cggtacggat atgcgtaagg aagacatcgg
      601 cggcgatttc acgctgaccg acggcgaagg caagcctttc agcctgagcg atttgaaagg
      661 caaggtcgtg attctgtctt tcggctttac gcactgtccc gatgtctgcc cgacagagct
      721 tttgacgtac agcgacacgt tgaagcagtt gggcgggcag gctaaggacg tgaaagtggt
      781 gttcgtcagc atcgatccgg aacgcgacac gcctgaaatc atcggcaagt atgccaaaca
      841 gttcaatccg gactttatcg gtctgacggc aacgggcggc caaaacctgc cggtcatcaa
      901 gcagcaatac cgcgtggttt ctgccaaagt caatcaaaaa gacgacagcg aaaactattt
      961 ggtcgaccac tcttccggtg cgtatctcat cgacaaaaac ggtgaggttg ccattttctc
     1021 gccttacgga agcgagccgg aaacgattgc tgccgatgta aggaccctgc tctgataaaa
     1081 ccgtatgccg tctgcaccgt cggcgcctgt tcagacggca ttattgtttc aaccgacaaa
     1141 ggacatccac accatgcagg ataatgcttt gaccatcgcc ttatccaagg ggcgcatttt
     1201 tgaggagacg ctgccgctgc ttgccgctgc cggcattgtt ccgactgaag agcctgaaaa
     1261 atcgcgcaag ctgattatcg ggacgaacca tgaaaacatc cgccttgtca ttgtccgcgc
     1321 aaccgatgtg ccgacttatg tccgctacgg cgcggcggac ttcggcattg cgggcaaaga
     1381 cgtgctgatc gaacacggcg gcacggggct ttaccggcct ttggatttgg agattgccaa
     1441 gtgccgcatg atggttgctg tgcgtaaagg gtttgattac gaagcagctt cgcaacccgg
     1501 atgccgtctg aagattgcca cgaagtatcc tgaaatcgcg gcatctcatt ttgccggcaa
     1561 gggtgtccat gtggacatta tcaaactgta cggctcgatg gaacttgcgc cgctggtcgg
     1621 cttgagcgat gcgattgtgg acttggtttc gacgggcaac accttgaagg caaacggctt
     1681 ggaagcagtc gaacacatcg tcgacatttc cagccgcctg gtggtcaaca aggctgcttt
     1741 gaaaacgaaa tacgcgctgc tggagccgat tattcaggcg ttcggcggcg cagtgaaggc
     1801 gaagtaagca ttcatttgaa taaagatgcg ttttcagacg accctatccg ttcccgccga
     1861 caggtcgtct gaaaatatca ccggcagtaa actgtatagg agaagttaaa atggttgcaa
     1921 aaataaaaaa attctcagat tcaacccttt ccgttttgaa taacggcgag cgtcggtttt
     1981 atgtctattg tctgaccgac ctgaaaaaag acaaaatcct ctacatcggc aaaggctgcg
     2041 gtaatcgtat cttcgagcat gaatgggttg ctagtcgttc acaagaccca gtctccggcg
     2101 agattatcga tcggaaactc aaagccatct ccaaatgcaa gaaactcggt cgctatatca
     2161 tcagctatca tctgactgaa gtcgaagcgc tcgccgccga atctgcctta attcattttg
     2221 ttaaatctgt cttgggtaaa aaactcaaaa ataaaattgc cgggcatggt ccgggtggta
     2281 ttagcgtaga agaactagat cgccgctttg gattctcttc tctcccactt gatgagatca
     2341 actccaacga gctaattctc gccatcaaaa tccacaatgc tttcgattta gatactgacg
     2401 aagaattaga ctaccttttc gacaaccaag acgatgccaa cctcaaatcg cgtacgttgg
     2461 gcaactgggt tatcggtaaa gatgttgctt caaaagtgaa atacgttatc ggcgttcaca
     2521 ccggtctgca aaacgctgtt gtcagtgcat acgaagtgga cggttttgaa acaatggttg
     2581 aggaaaccaa aaacggtaga aaacaatccc gttaccgttt ccgcactacc tctcgtagcg
     2641 aagaggtatt agccaaactc ggtctgcaac aaaaatgcct gcccgaattg aagtttggta
     2701 gcgggggaga aaaagcgtat atcagaccca aaacagagac agaaactgaa caagagaata
     2761 ttcagacgac ccccaatcca aaaataaaaa aggaaaaaac caaatcatga aaaaactcaa
     2821 cacccaatcg cccgatttcc aagccggact caaagccctg ctggcttttg aaaccgcgca
     2881 aaaccccgaa accgaacgca tcgtcgccga catttgcgcc gacgtgcaaa agcgcggcga
     2941 tgcggctttg attgaataca ccaacaaatt cgatcagaca aacgctaaaa gcatcgatga
     3001 tttaatactc acgcaagccg atttgaacgc ggcgttcgag cgcattccga acgacgttca
     3061 gacggcattg cagaccgccg cccgccgtgt cgaaagctac caccaacgcc aaaaaatgga
     3121 atcgtggagc tacaccgatg aagacggcac gctgttggga caacaaatca caccgcttga
     3181 ccgcgtcggc atttacgtcc ccggcggcaa ggcggcgtat ccgagttccg tcatcatgaa
     3241 cgccatgccc gcccacgtcg caggtgtgaa agaaatcatc atggtcgtgc cgacaccaaa
     3301 aggcgaacgc aacgacatcg tacttgccgc cgcatacgtc gccggcgtaa ccaaagtctt
     3361 caccgtcggc ggcgcgcagg cggttgccgc cctcgcctac ggcacggaaa ccatccccca
     3421 agtcgataaa atcaccggtc cgggcaacgc cttcgtcgcc gccgccaaac gccgcgtgtt
     3481 cggcgtggtc ggcatcgaca tggtggcggg gccgtctgaa atcctggtca tcgccgacgg
     3541 cacgacacct gccgattggg tggcgatgga tttgttcagc caggccgaac acgacgaaat
     3601 tgcccaagcc atcctcatcg gcacgtcgca agcgtatctc gacgaagtag aagccgctat
     3661 ggaccgcctg atcgaaacta tgccgcgccg cgacatcatc gaagcctcgc tcggcaacag
     3721 gggcgcgatg atactcgcca aagacttgga cgaagcctgc gaaatcgcca actacatttc
     3781 ccccgaacac ttggaactgt cagtcgaaaa cccgcaggaa tgggcgaaaa aaatccgcca
     3841 cgccggtgcg attttcatgg gacgctacac cggcgaaagc ctcggcgact actgcgccgg
     3901 tccaaaccat gtgttgccca ccagccgaac cgcccgcttt tcctcgcctt tggggacata
     3961 tgatttccaa aaacgctcca gcctgattca ggtttcggaa cagggcgcgc aaaaattagg
     4021 cgaaaccgcc agcgtgctgg cacacggcga aagcctgacc gcccacgccc gcgcggcaga
     4081 gttccgtatg aaataatgcc gaaacggcgt acaggcatat tccaaccatt aaggaaacac
     4141 gatgaaatcc gtccgctcct tcatccgcga cgacatacaa gctatgtcgg catatcagat
     4201 tgccgacgtt ccgcccggct ttgccaaact cgattcgatg gaaagtcccg tccacccttt
     4261 tgccggacat gaaacgctgt tgcaggaatg gcaggcacgg cttgccgccg cgcccatcca
     4321 tctttacccc aatccctccg gcagcggttt acaggaagca ttacgttcgg cgttcgacat
     4381 tcccgactgc gccgacatcg cgctgggcaa cggttcggac gaactgatac agttcatcac
     4441 gatgctgacc gccaaaccgg gcgcggcaat gttggcagcc gaacccagtt tcgtcatgta
     4501 ccgccacaac gccgcgctgt acggcatgga ttatgtcggc gttccactga acggagattt
     4561 caccctcaac ctgcccgccg tcctcgaagc cgtcaggaaa caccgccctg ccctgacctt
     4621 tatcgcctac cccaacaacc ccaccggcgt atgcttcacg cgtgccgaaa tcgaagccgt
     4681 catcgaagct tcagacggca tcgtcgtcgt cgatgaagcc tacggcgcat tcaacggcga
     4741 cagcttcctg ccgcaggcag gcaggattcc caacctgata gtcttacgca ccctcagcaa
     4801 aatcggtttt gccggactgc gtatcggtta tgcggcaggc tgccccgaag tcatcggcga
     4861 actgcaaaaa atcctgccgc cctacaatat gaaccaattg agcctgacca ctgccaaact
     4921 cgccctgcgg cactacggca ttatctctgc caacatcgac agcctgaaaa acgaacgcga
     4981 acggatgttc gccgaattgg gcaaaatatg ccgtctgaac acctttccaa gtcaggcaaa
     5041 cttcattacc atacgcgtac ccgatgccga tttgttgttt gacacgctca aacaaaaccg
     5101 catcttggtt aaaaaactgc atggcgcgca cccgcttttg gaacactgcc tgcgcattac
     5161 cgtaggcagc cccgcacaaa acgatgccgt tctcaacatc attcgccaac tttactgcca
     5221 accaacggat ttcctatgaa tttgactaaa acacaacgcc aactgcacaa ctttctgacc
     5281 ctcgcacaag aagcaggttc gctgtccaag ctcgccaaac tctgcggcta ccgtaccccc
     5341 gtcgcactct acaaactcaa acaacgcctt gaaaagcagg cagaagaccc agatgcacgc
     5401 ggcatccgtc ccagcctgat ggcaaaactc gaaaaacaca ccggcaaacc caaaggctgg
     5461 ctcgacagaa aacaccgcga acgcactgtc cccgaaaccg ccgcagaaag caccggaact
     5521 gccgaaaccc aaattgccga aaccgcatct gctgccggct gccgcagcgt taccgtcaac
     5581 cgcaatacct gcgaaaccca aatcaccgtc tccatcaacc tcgacggcag cggcaaaagc
     5641 aggctggata ccggcgtacc cttcctcgaa cacatgatcg atcaaatcgc ccgccacggc
     5701 atgattgaca tcgacattag ctgcaaaggc gacctgcaca tcgacgacca ccacaccgcc
     5761 gaagacatcg gcatcacact cggacaagca atccggcagg cactcggcga caaaaaaggc
     5821 atccgccgtt acggacattc ctacgtcccg ctcgacgaag ccctcagccg tgtcgtcatc
     5881 gacctttccg gccgccccgg actcgtgtac aacatcgaat ttacccgcgc actaatcgga
     5941 cgtttcgatg tcgatttgtt tgaagaattt ttccacggca tcgtcaacca cagtatgatg
     6001 accctgcaca tcgacaacct cagcggcaaa aacgcccacc atcaggcgga aaccgtattc
     6061 aaagccttcg ggcgcgccct gcgtatggca gtcgaacacg acccgcgcat ggcaggacag
     6121 accccctcga ccaaaggcac gctgaccgca taaaaaacca taccgtctga aacacccgca
     6181 ggcttttcag acggtatcgg aacagataag attacactac actacaaaca gaaaaggagt
     6241 aaacatcatg tccgcaaacg aatacgcaca aatcggctgg ataggcttag ggcaaatggg
     6301 tctgcctatg gtaacgcggc tcttggacgg cggcatcgaa gtcggcgtat acaaccgctc
     6361 gcccgacaaa actgccccca tctccgccaa aggcgcaaaa gtttacggca acaccgccga
     6421 actcgtccgc gactatcccg tcattttcct gatggtttcc gactatgccg ccgtgtgcga
     6481 catcctgaac ggagtccgcg acggattggc cggcaaaatc atcgtcaaca tgagcaccat
     6541 ctccccgacc gaaaacctcg ccgtcaaagc acttgtcgaa gccgcaggcg gacagtttgc
     6601 cgaagcaccc gtttccggat cggtcgggcc cgccaccaac ggcacgctgc tgattctgtt
     6661 cggcggcagc gaagccgttt taaacccgct gcaaaaaata ttttccctcg tcggcaaaaa
     6721 aaccttccat ttcggcgatg tcggcaaagg ttcgggcgcg aaactcgtct tgaactcgct
     6781 cttgggcatt ttcggcgaag cgtacagcga agcgatgctg atggcgcggc agttcggcat
     6841 cgataccgac accatcgtcg aagccatcgg cggctcggca atggactcgc ccatgttcca
     6901 aaccaaaaaa tccctgtggg caaaccgcga attcccgccc gccttcgccc tcaaacacgc
     6961 ctccaaagac ctcaacctcg ccgtcaaaga gcttgaacag gcaggcaaca ccctgcccgc
     7021 cgtcgaaacc gttgctgcca gctaccgcaa agcagtcgaa gccggctacg gcgaacagga
     7081 cgtttccggc gtttacctga aactggcaga acactgattg ccttttccaa acacaatgcc
     7141 gtctgaacat atttcagacg gcatttttat caccccacgc ttaaaatcag tcccgattat
     7201 ga
//
LOCUS       DAXHNE010000100         7152 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.100, whole genome shotgun sequence.
ACCESSION   DAXHNE010000100 DAXHNE010000000
VERSION     DAXHNE010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.100"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            152..1090
                     /locus_tag="ACFM4Z_001516"
     CDS             152..1090
                     /locus_tag="ACFM4Z_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFC6021533.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYSSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            1218..1511
                     /gene="hfq"
                     /locus_tag="ACFM4Z_001517"
     CDS             1218..1511
                     /gene="hfq"
                     /locus_tag="ACFM4Z_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFC6021534.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENKPQAAPASTLVQVETVQQPAE"
     gene            1596..2162
                     /locus_tag="ACFM4Z_001518"
     CDS             1596..2162
                     /locus_tag="ACFM4Z_001518"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6021535.1"
                     /translation="MLLKNILPFAHCLLRQALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCRLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTHTETSIPALCATLSLLKEGGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLRYGFTNRKNSPPYLLAFEKLRQK"
     gene            2216..2590
                     /locus_tag="ACFM4Z_001519"
     CDS             2216..2590
                     /locus_tag="ACFM4Z_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HFC6021536.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRVFLLWKNPEKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            2600..3094
                     /gene="folK"
                     /locus_tag="ACFM4Z_001520"
     CDS             2600..3094
                     /gene="folK"
                     /locus_tag="ACFM4Z_001520"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFC6021537.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPHLTLPHPRAHERSFVIRPLAEILPDFILGKHGKVTELSKQLGNQGIRL
                     LPEE"
     gene            complement(3230..3703)
                     /locus_tag="ACFM4Z_001521"
     CDS             complement(3230..3703)
                     /locus_tag="ACFM4Z_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFC6021538.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYISLSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDRKDSAYKRALSALSRKL
                     GRCKDKLHTAVQPVLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAML"
     gene            complement(3923..4660)
                     /gene="ubiE"
                     /locus_tag="ACFM4Z_001522"
     CDS             complement(3923..4660)
                     /gene="ubiE"
                     /locus_tag="ACFM4Z_001522"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFC6021539.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKSGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(4703..5071)
                     /locus_tag="ACFM4Z_001523"
     CDS             complement(4703..5071)
                     /locus_tag="ACFM4Z_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HFC6021540.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQLVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            5191..6810
                     /gene="mnmC"
                     /locus_tag="ACFM4Z_001524"
     CDS             5191..6810
                     /gene="mnmC"
                     /locus_tag="ACFM4Z_001524"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFC6021541.1"
                     /translation="MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITQAEAEKIAGIPLSVPYDHPSCGLYWQHGVW
                     LNPPTFVRALLSHPLIGLHEDTPLTDISHDGEKWIASTPNGTFTATHIIYCTGAHSSC
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAQLSPALAESLFAANPNPQKYQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAVAAEILG
                     LPHPLSKRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(6924..7041)
                     /locus_tag="ACFM4Z_001525"
                     /pseudo
     CDS             complement(6924..7041)
                     /locus_tag="ACFM4Z_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain protein"
BASE COUNT         1919 a         2241 c         1433 g         1559 t
ORIGIN      
        1 ttccgattta aaaacagaat gttataaaac ccaatcccca aaacacaaca agaccgcccg
       61 cctacgggca gtctcctgtc agacgacata ctttacagat ggctgttttt tcaacaaaat
      121 aacgccaata ctcaaaaata tggaatcaaa aatgtccatc catactctga aacgcctgcc
      181 ctcatcgctg ctgctcggtc tctgcctttc cctgccgtca gcccaccttt ttgccgacaa
      241 cgacatttta gggcaatttt tagaacagaa catgcttacc tcctccgatc cgatagaaat
      301 attcgccgaa agcacgatac accccaccaa cacccaagcc attacaggcg gtctgattct
      361 ctcctcacag tctgccctgg tcgtcaacaa caaaaccgga cagatactgt atcagaaaaa
      421 cgccgacagg attatgccca tcgcctccat ttccaaactg atgagcgcga tggtcgtttt
      481 ggatgcaaac ttggacatga acgaaaccgt taccattacg cccgacgaaa tcgaccgcat
      541 caaagggacc ggcagccgtc ttgccatagg tacggcactt acacgcaaag aactgctgca
      601 cctgagcctg atgagcagcg aaaaccgcgc cacccatgca ttgggcagaa cctaccccgg
      661 cggcatgggc gcatttgtcg ccgctatgaa ccgcaaagcc caaagcctgg gcatgtacag
      721 cagccgcttt tacgaaccga ccggactcaa cttccaaaac gtttccaccg ccaaagacct
      781 gagccttatg gtcaacgccg ccgcccaata tccgcaaatc cgcaccaact cgacttccaa
      841 ctacgcctcg gtacagacca aaaacgggca gcagaactac aaaaactcca atgcgctggt
      901 cagggaaggc atgtggaaca tcgaattgca gaaaaccggc tacatacgcg aagcaggcag
      961 gtctatggtt gtcaaagcca acattcaaaa ccaacccgtt accatcgtat tgctgaactc
     1021 tcccacatcc gccacacgcg tcaacgacgc ccgcaaaatc gaatcatgga tgctgcagca
     1081 acgctcctga catacaaatg cccggcggaa aaccgcccaa acacaaaaaa attggtaatt
     1141 tttcctattc aaggctacaa ttcgacacgc acagggcatc tgcccgattt cacaatcata
     1201 atagcggagt taaaaatatg acagctaaag gacaaatgtt gcaagatccc ttcctgaacg
     1261 ccctgcgtaa agagcatgtt ccggtttcga tttacctggt caacggcatc aaactgcaag
     1321 gtcaggttga atctttcgac caatacgtcg ttcttctgag aaacacttcc gtcacccaaa
     1381 tggtttacaa acacgccatt tccactatcg taccggctcg ctccgtcaac ctccaacatg
     1441 aaaacaaacc ccaagccgca ccggcttcga cccttgtcca agtggaaacc gtccagcagc
     1501 ctgccgaata atccgcacga agcatgacgt gtcatatctt tcaatacctt accggacaac
     1561 ggtaaggtat ttttattttc agacggcatt tcaaaatgtt attgaaaaac atccttccat
     1621 tcgcccattg ccttttgcgg caggcacttc ccgaaggcgg caatgctttg gacggcaccg
     1681 ccggcaacgg acacgacacc cttttcctcg cacaaaccgc aggcatccgg gggaaagtgt
     1741 gggcattcga catccagccg caagccctga gccgcactcg atgccgtctg aaagaagcga
     1801 gatacgagaa tgtgcaactc atcttagacg gacacgaaaa cctgaaacaa tatgtttcag
     1861 aaccactaaa tgccgccatt ttcaactttg gctggctgcc cggcggagat aaaagcctta
     1921 ccacccatac ggaaaccagc attcccgccc tttgcgcaac cctctccctg ctgaaagaag
     1981 gcggtatgct tattgccgtc ctctatccgg gacacgaaaa cggcaaacag gaggcagaag
     2041 caatcgaaca atgggcaaaa aacctgcctc aagaacagtt tgccgttttg cgttacggct
     2101 ttaccaaccg gaaaaacagc ccgccctatc tgttggcatt tgaaaagtta cgtcaaaaat
     2161 aaatgtttgc ggtaaaataa gccgattcca acccttattt tgataagagc cgaccatgca
     2221 gtatctgatt gtcaaataca gccaccaaat cttcgttacc atcaccattt tggtattcaa
     2281 catccgtgtt ttcctacttt ggaaaaatcc agaaaagccc ttggtcggct tttggaaggc
     2341 actgccccac cttaacgaca ccatgctgct gtttacagga ttgtggctga tgaaaattac
     2401 ccatttctcc ccgttcaacg cgccttggct cggcacaaaa atcctgcttc tgctcgccta
     2461 tatcgcattg ggtatgatga tgatgcgcgc ccgtccgcgt tcaaccaagt tctacaccgt
     2521 ttacctgctc gctatgtgtt gcgtcgcctg tatcgtttac cttgccaaaa ccaaagtcct
     2581 gccattctga aacactgcca tgaacaacag acattttgcc gtcatcgccc tgggcagtaa
     2641 tcttgaaaac cctgcccaac aggtacgcgc cgcattggac acgctgtcgt cccatcctga
     2701 catccgtctt aaacaggctt cctcactgta tatgaccgcg cccgtcggtt acgacaatca
     2761 gcccgatttc gtcaatgccg tctgcaccgt ttccaccact ctggacggca ttgccctgct
     2821 tgccgaactc aaccgtatcg aggctgattt cggacgcgaa cgcagcttcc gcaacgcgcc
     2881 gcgcacattg gatttggaca ttatcgactt tgacggcatc tccagcgacg acccccacct
     2941 taccctgccg catccgcgcg cgcacgaacg cagtttcgtc atacgtcctt tggcagaaat
     3001 cctccctgat tttattttgg gaaaacacgg aaaggttacc gaattgtcaa aacagctcgg
     3061 caatcaaggt atccgccttt tgcccgaaga ataatccgcc acacggaaat gccgtccgaa
     3121 caccggtttc agacggcatt tttcctttgc cgccaacacg cgtgcaaaaa aaatcgcccc
     3181 ttgggaaagg ggcgcaaaag gaacacaaac cactaccaaa actttaaatc tacaacattg
     3241 cctgccgcat actgtatccg acaggatata aagccctcac taaatcgttt cgagaaatcc
     3301 aaacttcttc atcgccgaca gaaaatctgc ctttctccgg taccagctcc aacagaaccg
     3361 gttgaaccgc cgtatgcaac ttgtccttac accgccccag ctttctggac aacgcggaca
     3421 gggcgcgttt gtaggcacta tccttgcggt caagctcccg cgcactgccg acccagctca
     3481 aacgaaggtt gcggctttca tcctcaatca gcaaaatgcc tgttttaact tcccccacct
     3541 cgggctcgca tgaaagcgaa atataatatt tgccatcgat gttgacatac gccgcctcat
     3601 gcacctgaca gttgcagccg gaagtggcaa gcagcgaata aacagaatcc cgatgcagtt
     3661 tcaaaatatc aagggaaatc tgtttaaaaa tactttccgg catttttccc tcctttcgtc
     3721 tgctgcgttg tttactggat tgactgcatt atacagacat ctcccaatct tgcaaatgat
     3781 aattgttcct attttttaaa aaatttataa attattgatg gatatgtgaa ttaattttaa
     3841 gatttaaaat ctctttctca aaaaaacaca aaaatgccgt ctgaaacgtg ttcagacggc
     3901 attggctgca cagccagtcc gtttaaaatt tcacgccctt atgtaacgca acgatccccg
     3961 cactcatatt atgatagtcc acactgtcaa atccggcatc cagcatcatt tgctttaaag
     4021 tttcctgatc ggggtgcatg cggatggatt cggcgagata ttgataactc tccgcatctt
     4081 tggcaatcag tttgcccatg accggcagca gcttgaacga ataaaaatca tacgcgcctt
     4141 cgagcggctt gtagattttg gaaaactcca acaccagcaa tgtcccgcca gatttcaaaa
     4201 cacgatacat ctctttcagc gcggcatctt tatgcgtcat gttccgcaag ccgaacgcca
     4261 cggaaaccaa gttgaaataa ttgtcaggga aaggcagttt ttccgcatcg gcaagcgaca
     4321 cgggcaaaat catgccttcg ttcaacagac ggtcgcgccc gacggtcagc atagaggaat
     4381 taatatcggt cagccaaacc tcgccttcct tgccgacccg tttcgcccaa ccgcgcgaca
     4441 aatcgcccgt accacccgca atgtccaaca ctttatcgcc ttttttcagg tgcgccgtgt
     4501 tgatggtgaa atgcttccac accctgtgca gccctgccga catcacatcg ttcataatgt
     4561 cgtagttttt ggcgacggag tggaacactt ccgccacttt tccggctttc tcatcttcgt
     4621 tgaccgtgct gaacccgaaa tgcgttttct gtccgcccat attctaacca cctccctgat
     4681 gccgccctac ggcatcccaa acttaacaat ctgattgctt ttcaaaacgg gaagcacccg
     4741 ccgccgccaa tttgtccaaa tattcctgcc acatcgcatc gtatccgtat gccagtctgt
     4801 gcagatacgc ccaatcgtaa agaccgctgt cgtgcccgtc cgaaaaactg attttcagcg
     4861 cgtactgtcc gacaagctgc aaatccgtga tttggacatc cgccttgccg gtctgcaaaa
     4921 catcctgtcc cacaccgtgt ccgcgcactt ccgcactcgg cgaatagacg cgtaaaaatt
     4981 cggcaggcag atttttaggt tcgtccccat aagtcaaaac caacacggta cggtttttca
     5041 acaaacggat ttcaacagga atattattca aaaaacacct cggttcggca tacaatatcc
     5101 ggcaggcgca ggcgtgcctt caaacggcgc gtattttaac agctttatcc cgataccgat
     5161 attcaaaccc acatttcaga cggcattgat atggacaatc tcgtatggga cggcgttccc
     5221 gacatccgca cactcgacca aaccatccgc aaacacgcac acccgctcaa cctgattgtc
     5281 tgcctccccg ataatcagat tcccgatttt caaaccgcac aagatgcttc ggacgcggaa
     5341 tgccgtctga agcaccgttt ggatcaggca acgcagtgcc tccagttcga cagcatcaac
     5401 ctcatcgaac acatcctgcc cgatgtccgc ttctggctgg ttcccccttc acgcacccgc
     5461 cgcctgcacg aacacttcca ccacatttcc tggcagaccg aagccatccc gcaaaccgaa
     5521 agcaaacccg acaaaccctg gtttgcactt ccacaaacat ccgaacggca aaaaccggaa
     5581 cacatcctcg ttatcggagc gggcatatcc ggcgcggcaa ccgcccacgc cttagcatca
     5641 cacggcattt ccgttaccgt attggaagcc cgaaaagccg cccaagccgc cagcggcaac
     5701 cgccaagggc tgctctacgc caaaatctcg ccgcacgaca ccgaacaaac cgaactgctg
     5761 cttgccggct acggctacac caaacgcctg ctcggacata tcctgcccga atccgaaacc
     5821 tggggcggca acggcatcat ccacctcaat tacagccgca ccgaacaaca acgcaatcac
     5881 gaattgggtt tgcaaaaaca ccataaccac ctctaccgca gcatcacgca ggcagaagcc
     5941 gaaaaaatcg ccggtatccc actgtccgtc ccatacgacc acccttcatg cggactctac
     6001 tggcaacacg gcgtatggct caatcctccc acattcgtcc gcgccctcct cagccatccg
     6061 ctgatcggac tgcacgaaga cacacccttg accgacattt cccacgacgg ggaaaagtgg
     6121 attgcaagca cgccaaacgg cacatttacc gccacacaca tcatctactg caccggcgcg
     6181 cacagctcct gcctgcccga aaccaacctc gccgccctgc ctctcaggca aatacgcgga
     6241 caaaccggcc tcacaccatc caccccgttt tccgaacaac tgcgttgcgc cgtttcaggc
     6301 gaaagctaca tcagcccgtc gtggcacgga ctgcactgct acggcgcgag ttttattccc
     6361 aacagcagcc ataccggatg gaacgaagcc gaagaagcct caaaccgcca agcattggca
     6421 cagcttagcc ccgccctcgc cgaatcattg tttgccgcca acccaaaccc ccaaaaatac
     6481 caagggcacg ccgccatacg ctgcgacagc cccgaccacc ttcccctagt cggcgcactc
     6541 ggcgacattg ccgccatgcg gcagacctac accaaactcg cgctggacaa aaactaccgc
     6601 atcgacaccc catgcccata cctgcctaat gcctacgtca acaccgccca cggcacacgc
     6661 gggcttgcca ccgcccccat ctgcgccgcc gccgttgcag ccgaaatcct aggcttgccc
     6721 catcccctct caaaacgcct gcgccacgcc ctacacccca accgcaccat catccgcgcc
     6781 atcgtcagaa ggaaggatct aaccccttaa gcctttgccg catgccgtcc cgaccacaga
     6841 aaatcatatt caaataaaca taccaatcct gacggtaaaa tatcgaaaac acccataacg
     6901 ttttcttccc aaaataagga gtaaggaatg cctaacaaat tgcctttgct gtccaagatg
     6961 aatgttccgt aacattcgaa gacttcctta accctgccag ccgtttcggt ttctgtaccc
     7021 cctgttagat aaggaaggaa tgaaaaagcc ctgttgattg tatgaagtaa agggcgttaa
     7081 tttttgttaa tcttcccttc tcaggaactc aatatataag gatgttaatt tttatttccc
     7141 ttctcgagga ct
//
LOCUS       DAXHNE010000101         7139 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.101, whole genome shotgun sequence.
ACCESSION   DAXHNE010000101 DAXHNE010000000
VERSION     DAXHNE010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7139
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.101"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            144..725
                     /locus_tag="ACFM4Z_001526"
     CDS             144..725
                     /locus_tag="ACFM4Z_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC6021542.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(787..1641)
                     /gene="folD"
                     /locus_tag="ACFM4Z_001527"
     CDS             complement(787..1641)
                     /gene="folD"
                     /locus_tag="ACFM4Z_001527"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFC6021543.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHMPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            1864..1941
                     /locus_tag="ACFM4Z_001528"
     tRNA            1864..1941
                     /locus_tag="ACFM4Z_001528"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1899..1901,aa:Pro,seq:tgg)
     gene            1966..2042
                     /locus_tag="ACFM4Z_001529"
     tRNA            1966..2042
                     /locus_tag="ACFM4Z_001529"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2000..2002,aa:Arg,seq:tct)
     gene            2070..2145
                     /locus_tag="ACFM4Z_001530"
     tRNA            2070..2145
                     /locus_tag="ACFM4Z_001530"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2103..2105,aa:His,seq:gtg)
     gene            2357..2863
                     /locus_tag="ACFM4Z_001531"
     CDS             2357..2863
                     /locus_tag="ACFM4Z_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HFC6021544.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGGALVLALNTDASVRRLGKGGDRPINPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            2860..4638
                     /locus_tag="ACFM4Z_001532"
     CDS             2860..4638
                     /locus_tag="ACFM4Z_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HFC6021545.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLDVQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLVELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRSDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            4751..5575
                     /locus_tag="ACFM4Z_001533"
     CDS             4751..5575
                     /locus_tag="ACFM4Z_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFC6021546.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(6338..7126)
                     /locus_tag="ACFM4Z_001534"
     CDS             complement(6338..7126)
                     /locus_tag="ACFM4Z_001534"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFC6021547.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRR
                     AGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDVGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPYTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
BASE COUNT         1523 a         1849 c         2144 g         1623 t
ORIGIN      
        1 aattatgtat ggcgtaaagg aagcgtttta acgccgtatt gggaaaatgt aaagttcttt
       61 atcgggattt ctttaagtat tggcgttttc ggtgtcgtgg cggtagaatc ggggtcagtt
      121 aagaacaatt atcaggaacg gatatggaag aaaaaaacga ctacaccgac gcggcatccg
      181 acaaccgcaa cgggcaggaa attgaagtcg gaattgccgg cgcaggcgac cggatacttg
      241 ccgcgctgct gaaccaattg tttacctttt tggttttgtt ggtgccgttt gccggactga
      301 ttgctttcgc catcaagaat gaaggcagga ttggcggcag tgaggaaata ttcgggctgc
      361 ttttgggcat gacttccttt tgggtaggac tggcgggtat tttggtttat accgtcgttc
      421 aaatctacta tatgagtcgg gacgggcagt cattgggtaa gaaaatcatg agaatcaggg
      481 tgttgaaaac cgacggccgc aatcccggtt ttgtcggcac ggttttggta cgcgaaatcg
      541 catggtcggt tttggttgcc attattgccg ccgttatcgg tcttgcagta ggtgacaacg
      601 gagaaaacgc catcaacctg ctggcattcc ttgccaactt tgtcctgctc tttatggtca
      661 aacgcgaccg ccgcacgctt tacgacatac tggcggatac ggttgtcgtc aagctgccca
      721 aataaacgga cacggcaaaa tgccgtctga aagcctttca gacggcattt ttatcttcag
      781 aaccgcttaa gcatcgtgca gcgaagccgc gtgcaaagtg ttttccatca atgtggcaat
      841 cgtcatcgga cccacgccgc cgggaacggg cgtaatcatc gccgcccgtt cttttgccgt
      901 ttcaaattcc acgtcgccgc acaggctgcc atcgtccaaa cggttgatgc ccacatcaat
      961 aacgaccgcg ccaggtttga tccattcgcc tttgacaaag ttcggaatgc ctacgccgac
     1021 caccaaaata tcggctccgg caacctcgtc tgtcagattt tcggttgcgc tgtggcagac
     1081 cgttaccgtt gcgcgcgcca gcagcagttc caaagcctgc gggcggccga cgatattcga
     1141 cgcgccgacc acgaccgctt ttttcccctt cggatcaatg ccgtaagctt ccaaaagcgt
     1201 catcacgccc ttgggcgtac acgggcgcat cagcggcatt ttgaccgcca gcctgccgac
     1261 attgtaagga tggaagccgt ccacgtcctt atccggcgaa atacgttcca aaaccgcctg
     1321 gctgtcgagg tgcttcggca gcggtagctg aaccagaata ccgtccactt cggaatcggc
     1381 attcaggcgg tcgaccagtg ccagcagttc ttcctgcgat gttgattcgg gcagctcgta
     1441 agacagtgat ttgatgccgc atttttggca ggcagttttc ttgttgcgga cataaaccgc
     1501 gctggcaggg tcgcctccga ccaaaaccac ggccaggcaa ggcatgtgca gattgtcctg
     1561 ttggcgttgc gccaccgctt cggcaaccgc ctgcaggcgt ttttgcgaaa cttctttacc
     1621 attgatcagt tgtgccgaca tatccgtcct tttgttaaac aggtagaaaa tgctgaaaat
     1681 tatcgcattt tccgtttgtt tttacagcag gatgccgaaa ataaatttgc cctgaaataa
     1741 aatacggctg tttggacgga ttaaatattt tttcaaatca aaaaactata tttttcatct
     1801 tggtgtcttg aaaataaatg ttgacgcatc tttccctgag ttgtatagtt cgctacttca
     1861 agtcggggcg tagcgcagcc cggttagcgc atctgctttg ggagcagagg gtcgtgagtt
     1921 cgaatcccac cgccccgacc aatcaatcag cacgaatcgg catgagcgcc cgtagctcaa
     1981 ccggatagag caccgacctt ctaagtcggg ggttacaggt tcgattcctg tcgggcgtgc
     2041 caagaatttg attgagaacc gtttttatgg tggctgtagc tcagttggta gagccccgga
     2101 ttgtgattcc ggttgtcgtg ggttcgagcc ccatcagcca ccccagataa aggcccgtac
     2161 ttttcggtac gggtcttttt ccgtttgcac gtactgtccg caccgtcgta tcggtgcggc
     2221 ggtatgccat ctgaaagctg ggccggtcat acggtaaccg cttcagacgg catttgcgcc
     2281 ttcggctttg cctttcgcat atgcttctgt atcatgtggg tttttctgtc ttttatggtt
     2341 tttgtcggag actttgatgg ttgacgcttg gtctgtccct gattttgaat cgaaaatctg
     2401 tccgcccgag gcgttggcgg cgcgtttggc gttgttgccg cgcccgctgg tgtttaccaa
     2461 cggctgtttc gacatcctcc acagggggca cgttacttat ctggcgcagg cgcgttcgat
     2521 ggggggcgcg ttggtgctgg cgttgaatac cgatgcttcg gtgcggcgtt tgggcaaggg
     2581 cggcgaccgc ccgattaatc ctttggagaa ccgtgccgcc gttgccgccg ctttggaaag
     2641 tgtggatttg gtaacgtggt ttgacgggga tacgccggcg gcgttgattg aggcggtcaa
     2701 acctgagatt ttggtcaagg gcggcgattg ggctgcggat aagattgtcg gtgcggcaga
     2761 aacgttggcg cgcggcggtc aggtgttttc aattccgttt ctgcaccaga cttcgacaac
     2821 gaagactttg gcaaaaatcc gtgcggcaga gggcggaaaa tgacggtttt gaagccttcg
     2881 cactggcggg tgttggcgga gcttgccgac ggtttgccgc aacacgtctc gcaactggcg
     2941 cgtatggcgg atatgaagcc gcagcagctc aacggttttt ggcagcagat gccggcgcac
     3001 atacgcgggc tgttgcgcca acacgacggc tattggcggc tggtgcgccc attggcggtt
     3061 ttcgatgccg aaggtttgcg cgagctgggg gaaaggtcgg gttttcagac ggcattgaag
     3121 cacgagtgcg cgtccagcaa cgacgagata ctggaattgg cgcggattgc gccggacaag
     3181 gcgcacaaaa ccatatgtgt gacccacctg caaagtaagg gcagggggcg gcaggggcgg
     3241 aagtggtcgc accgtttggg cgagtgtctg atgttcagtt ttggctgggt gtttgaccgg
     3301 ccgcagtatg agttgggttc gctgtcgcct gttgcggcag tggcgtgccg gcgcgccttg
     3361 tcgcgtttag gtttggatgt gcagattaag tggcccaatg atttggttgt cggacgcgac
     3421 aaattgggcg gcattctgat tgaaacggtc aggacgggcg gcaaaacggt tgccgtggtc
     3481 ggtatcggca tcaattttgt cctgcccaag gaagtagaaa atgccgcttc cgtgcaatcg
     3541 ctgtttcaga cggcatcgcg gcggggcaat gccgatgccg ccgtgctgct ggaaacgctg
     3601 ttggtggaac tggacgcggt gttgttgcaa tatgcgcggg acggatttgc gccttttgtg
     3661 gcggaatatc aggctgccaa ccgcgaccac ggcaaggcgg tattgctgtt gcgcgacggc
     3721 gaaaccgtgt tcgaaggcac ggttaaaggc gtggacggac aaggcgtttt gcacttggaa
     3781 acggcagagg gcaaacagac ggtcgtcagc ggcgaaatca gcctgcggtc cgacgacagg
     3841 ccggtttccg tgccgaagcg gcgggattcg gaacgttttc tgctgttgga cggcggcaac
     3901 agccggctca agtgggcgtg ggtggaaaac ggcacgttcg caaccgtcgg tagcgcgccg
     3961 taccgcgatt tgtcgccttt gggcgcggag tgggcggaaa aggcggatgg aaatgtccgc
     4021 atcgtcggtt gcgccgtgtg cggagaattc aaaaaggcac aagtgcagga acagctcgcc
     4081 cgaaaaatcg agtggctgcc gtcttccgca caggctttgg gcatacgcaa ccactaccgc
     4141 caccccgaag aacacggttc cgaccgctgg ttcaacgcct tgggcagccg ccgcttcagc
     4201 cgcaacgcct gcgtcgtcgt cagttgcggc acggcggtaa cggttgacgc gctcaccgat
     4261 gacggacatt atctaggggg aaccatcatg cccggtttcc acctgatgaa agaatcgctc
     4321 gccgtccgaa ccgccaacct caaccggcac gccggtaagc gttatccttt cccgaccaca
     4381 acgggcaatg ccgtcgccag cggcatgatg gatgcggttt gcggctcggt tatgatgatg
     4441 cacgggcgtt tgaaagaaaa aaccggggcg ggcaagcctg tcgatgtcat cattaccggc
     4501 ggcggcgcgg caaaagttgc cgaagccctg ccgcctgcat ttttggcgga aaataccgtg
     4561 cgcgtggcgg acaacctcgt cattcacggg ctgctgaacc tgattgccgc cgaaggcggg
     4621 gaatcggaac atacttaaaa aatacaccct ttttcaacac ggggcggcat ccgtccgaat
     4681 ccgtccggat tctgccgccc gacagactgc cgcccaggcg ggcggggttt caaacattga
     4741 ggaaattcta atgaaatggc tcttcaccct tttggcggta ctcaatattg ccgtattcgg
     4801 cggcacggtg ggctataaat tggccgttaa agcgacgggc ggcgtgccgg aaaaccgggc
     4861 cgtcgaaaac acgccccccg caacgcccgc cgccgacaat gcggcggcat ccgtggagga
     4921 tacggcggca ctgctcaaac cgggcgacat cctgaccgaa gagcaggccg agcagttgcg
     4981 cttgaaaaag gaagcggaac agaaaaaact gagggagaaa aaacagcgtg aagaaaaagc
     5041 ccgccgcgaa aaactcgccg cagaaaaggc gcaggcggaa cgcgaaaacg gcgcggcgga
     5101 tgccttatgc gccgcgcagg caagcctcac gatggacgaa gatgactacc accgcatcaa
     5161 aggacttttg ggcaaatggt cgcacgttgc cagcaggagc gtcgaaaaac gcaccgccaa
     5221 agccaaacct gccgacaaaa cctaccgcgt cgtcctgccc gtttccgccg atgccgaaaa
     5281 tcaggcggcg gagctgtctg ccaaaggttt caaccccata ccgtttgacg gcgcattgag
     5341 tttgggtgtc ggcaacagcc gggaaaacgc ccaagccctg caaaaccggc ttgccggcgc
     5401 cggattcggc ggggcgcata ttgtcgaaca ctttgccgaa gccgacaggc aggacgattc
     5461 tttgagcgtg tcgcgtatga cggttttgtt taccggcgtg aatgccgccg atgcggacga
     5521 aatccgtaaa atcacgtccc tatacggcaa actgaacctc aagtcttgca aataggcgcg
     5581 aaagccggac gataaacgcc aaacccaccg cgaaggtggg tttggcgttt cggttttgag
     5641 tatctgatat ataaaaaggg aatgtatgtc tcgtatatgc cgactccaag tgtgaaagtg
     5701 atgatgggga aatatggtgt ttaatggttc ggaacggacg gcttccgagt ggttttgatt
     5761 atacgctaat aataataatt atcaataaaa atttaaaaat aaatgcagat tttttgtaat
     5821 acgatgaaaa ataaacaaat taacctgtcc gccgatgttt gtgattcggg tcggcaaact
     5881 ttcattgtgt cggacaaatc ggggtcggca gtcatagctg agggcttcgt gcctgacgga
     5941 aataaggttg cccgtgtttg aacatccgtc cgattgggcg cggacagggt tgtcattcgg
     6001 gggtgtttga gcgtggattc ggggatgcga aagcggtaaa accgttgcgg atgaaggctt
     6061 acggacggct ttaggtattt ttaagtgtat tcaaacaaca acaaaacgct tcttctattg
     6121 ccgtatttag ggcttgcgcg gcagcctgca caccgtggag gccgagtgtt tcgttggttg
     6181 cggcatcggg acgggcggca aggttttccg gccggttttt ccattttttc cgacaaagcc
     6241 gtgatgccgg ggaatggcgg tttgtccgtt tcggaccgcc gccgttttga gccgcgatgc
     6301 cgtctgaagc cttcagacgg catttttgcc gcctttttca atattccgcc gaatgccaaa
     6361 acggctgtcc gactgtcggc gtgctggctt gcgctttgtc gcgtgcttcg accggtccgg
     6421 cttcaaatgc cagccgtccg gattcgacgg cgagtgcgaa ggcgcgtgcc atattgactg
     6481 gatcgccgct gcgggaaacg gcggtgttca aaagcacgcc gtcaaagccc cattccatca
     6541 cttgtgccgc ctgtgagggc aaacccaagc ccgcgtcgat aatcagcggc gtgtagggca
     6601 ggcgttcgcg caggacgttc aacgcgtagg cgtgaaccgc gcccaaaccc gtgccgatcg
     6661 gggccgccca cggcatcaac gcctgacagc ccacatcgag caggcggcgg caggcaatca
     6721 ggtcttcggt gcaataaggc agcactttga agccgtcttt aatcaggatt tccgccgctt
     6781 cgacaagttg gaacacatcc ggctgcaagg tgtcgtcgtc gccgatgagt tcgagtttaa
     6841 tccaatcggt ttcaaacact tcgcgcgcca tttgcgccgt cgttaccgct tcctgcacgc
     6901 tttggcagcc tgccgtgttc ggcaggacgg gaacgccggt ttcttgaagc agcgaccaaa
     6961 acccctgacc gtgtgcctcc ccaccgcttc ccgcgcggcg cagcgagacg gtaatcatcg
     7021 caggccgggc ggttcggacg gattgtttga ggatttcagg ggtcgggtag gcagccgtgc
     7081 cgagcagcag ccgcgagggg aaagtttctc cgtatagggt gagcataatg ggttccttt
//
LOCUS       DAXHNE010000102         7095 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.102, whole genome shotgun sequence.
ACCESSION   DAXHNE010000102 DAXHNE010000000
VERSION     DAXHNE010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7095
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.102"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(71..430)
                     /gene="rnpB"
                     /locus_tag="ACFM4Z_001535"
     ncRNA           complement(71..430)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACFM4Z_001535"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(509..1612)
                     /gene="prfB"
                     /locus_tag="ACFM4Z_001536"
     CDS             complement(509..1612)
                     /gene="prfB"
                     /locus_tag="ACFM4Z_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFC6021548.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVVGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1715..2608)
                     /locus_tag="ACFM4Z_001537"
     CDS             complement(1715..2608)
                     /locus_tag="ACFM4Z_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFC6021549.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHIGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRRIQQFPIKFAAIATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2950..3399
                     /locus_tag="ACFM4Z_001538"
     CDS             2950..3399
                     /locus_tag="ACFM4Z_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC6021550.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3473..3781
                     /locus_tag="ACFM4Z_001539"
     CDS             3473..3781
                     /locus_tag="ACFM4Z_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021551.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAADKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3898..4740
                     /locus_tag="ACFM4Z_001540"
     CDS             3898..4740
                     /locus_tag="ACFM4Z_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HFC6021552.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFIEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEC
                     RRAA"
     gene            4730..5476
                     /locus_tag="ACFM4Z_001541"
     CDS             4730..5476
                     /locus_tag="ACFM4Z_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HFC6021553.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5498..6085
                     /locus_tag="ACFM4Z_001542"
     CDS             5498..6085
                     /locus_tag="ACFM4Z_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFC6021554.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6091..6270
                     /locus_tag="ACFM4Z_001543"
     CDS             6091..6270
                     /locus_tag="ACFM4Z_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HFC6021555.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            6502..6942
                     /locus_tag="ACFM4Z_001544"
     CDS             6502..6942
                     /locus_tag="ACFM4Z_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFC6021556.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1804 a         1892 c         1621 g         1778 t
ORIGIN      
        1 aaacacaacc taaataaaaa tgccgtctga accatatttc aggtttcaga cgacatttgc
       61 gtgtcggatg cacaccggac aggcggtaag ccgggttctg tctcggacag tcattcctct
      121 aggcataccg ttaccggtat gctcaagcaa cctacccgaa cgctcggcgg gcagcgtcat
      181 tgcgttctgt ttggtcttgc tccgaatggg gtttggcctg ccgcatattg ttaccaaatg
      241 cgcggtgcgc ccttaccgca ccttttcacc cttgcctgtg ctgccaaagc agccatcggc
      301 ggttttgctt tctgttccac tttccgtcgc gttaccgcgc ccggccgtta accggcattc
      361 taccctgcgg agcccggact ttcctccccg tatgccttac gcgatacgcg gcgactgtct
      421 gcccgtcccg tgtgcggcgc ggattataac acgaaacgca aaaatgccgt ctgaaacggt
      481 acaggtttca gacggcatac agcctaaact acacgccctg tttcaggctg gcttcgataa
      541 agccgtccaa atcgccatcc aatacggctt tggtgttgcc gacttcgtag cctgtacgca
      601 agtctttgat acgtgaggaa tccaaaacat acgaacggat ttggctgccc caacctacat
      661 cggatttacc ttcttccaac gcctgtttct cttcattgcg tttgcgcatt tccaattcat
      721 acagtttgga cttcaacatt tccatcgcgg cggctttgtt ggcgtgttgc gaacggtcgt
      781 tttgacattg caccacaatc cccgtcggct cgtgggtaat gcgcacggcg gagtcggttt
      841 tattgatgtg ctgaccgccc gcgcccgatg cgcgataggt gtcgatacgc aaatcggcgg
      901 ggttgatttc gatttcaatg gaatcatcga tttcagggta aacgaacacg gaggcaaacg
      961 aagtatggcg tttgttgttc gagtcaaacg gtgaatagcg caccaagcgg tgaacgccgg
     1021 tttcggtacg cagcaaacca taggcgtatt cgccttccac tcggatagtc gcgcggttga
     1081 tgcccgcgat ttcgccgtcg tcttcctcaa ggatttcgat tttgaagcct ttgcgctcgg
     1141 cgtagcggct gtacatacgg aacagcatcc ccgcccagtc ttccgcttcc gtaccgcccg
     1201 cgcctgcggt gatgtcgata aagcagttgt tcggatcggc aggctggttg aacatccgtt
     1261 tgaactccaa atccgccatc tgtttttcca gccccgccac atcttcctgc acggcggcga
     1321 aaccttcttc gtcgttttct tcgacggtca tttcaatcag catacggttg tcttcgatgc
     1381 ccgaagcgat gttgtcgagc gtcaacacga tgccttcgag gattttgcgc tctttgccga
     1441 tttcttgggc gcgtttcggg tcgttccaaa gttcggggtc ttcggaaaga ccgacaactt
     1501 cttccaatcg gtctttctta ccctgataat ccatataaac gcggatgtct tcgctgcgct
     1561 tctccaagtc gttcagggtg ttgttgagct ggttgattac ttcggcttcc atgattcttt
     1621 tgttctttca aaattttagg ggcgtattgt acgggattcg ggtatttttt tctatggata
     1681 aagccttctg gaaacacgtt cggacggcat agcgtcaata acggtatgcc gccagtttgc
     1741 gtttgatttc aggcaatgcg gcacgtgcag cctcctcgcc caaccggatg gcgcgttttt
     1801 tctgatcgaa tcctccgact gcacccaaat ccaaaacctg cggtttgata accacatccg
     1861 cctgccccaa ctcgttttgc agtgcggaaa cgctcattac gttcagcgtc tgatcgagat
     1921 aagagaagaa gccttggctg atgtttttgc ccggacgggc ggaaatatcg acggcaatca
     1981 cgaaattcgc cccctgccgc cgggcggcac tgacgggcac gggctgcgac agaccgccgt
     2041 caacatatgt atgcctgccg atgataacgg gttggaacac attgggaatg gcggcggaag
     2101 cgcgcacagc ctgcccggca ttcccctgat tgaaagcgac ggccttgccg gtttcaaaat
     2161 cagtagcaat agcggcaaat ttgatgggaa actgctgaat ccgcctgccg ccgacttttc
     2221 ggttgatgta attttgcagc ttttcgcctt tgataaaacc actggtggac aaggttaaat
     2281 cgactaaatc cgttttacct aaaatctcgg cttccaattc aaggcggtcg ggcgacatac
     2341 ccgatgcaaa aaggctgccg actatcgaac ctgccgatgt gccggttact acttttacag
     2401 gaataccgtt ttctttcaaa accttaataa tgcctatatg ggcaaatcct ttagacgcgc
     2461 cgccaccgag tgccaaaccg accactgcgg cgggtttggc ggtttgcacc ggcttgcggg
     2521 cggcattgtt tcccgccgtg ccgcaggcgg caagcaacgc ggcggcggcg attgccaaaa
     2581 gcggtctgat ttttgaaaac gttaccatat tttccattcc tttatatatc gcaccccgtc
     2641 aaaaagaggg attgcttttc ttaacaaccc cctttgacag ccaagcaaat gggggctttg
     2701 ttaagtcatc atcaaaatta atatttcttt ttttccttta cggaaattat atttgaaggc
     2761 atactatcca aggcgggaat tatctcacaa caccgccgtt atccaaatat cccgcctttt
     2821 tcctttcctt tccatcaaaa tactttcttt ttatattcat taacttgtta aatcattggc
     2881 tgccgggtgt cagtttttcc gacaaaatcc gtctaatggg gtatcaacag aaccaaaaca
     2941 ggaacactta tgaaaatcgg aacaacttgg cagacggcat ccgctatgct ggttttgcgt
     3001 ctgtttgccg catatgaatt tttggaatcg ggtttgcaaa aatggaacgg ggagaattgg
     3061 ttttctgaaa tcaacgatca attcccattc ccgttcaact tgctgccgga cgcgttaaac
     3121 tggaatctcg ctatgtatgc ggagctttta ctgcccgtac tgttgctttt gggtttggca
     3181 acgcgtctgt cggcattagg gctgatggtc gttaccgccg tcgcttgggc tgcggttcac
     3241 gccggttcgg gttacaatgt ctgcgacaac ggttataaaa tggctttaat ttatatcgtg
     3301 gtattaatcc cgctgctttt ccagggtgcg ggcggatggt cgctggatac gctgctgaaa
     3361 aaacggtttt gcccccaatg ccgtctgaag caagattgat tcagtcgtgg aatctgactt
     3421 taaacattcc aaccttatct cgttaacttg atattctgaa aaggaaatag aaatgaacaa
     3481 aaacattgct gccgcactcg ccggtgcttt atccctgtct ttggccgccg gtgcagttgc
     3541 cgccgacaaa ccggcaagca acgcaacagg cgttcataaa tccgcccatg gctcttgcgg
     3601 cgcgtccaaa tctgccgaag gttcgtgcgg cgcggctggt tctaaagcag gcgaaggcaa
     3661 atgcggcgag ggcaaatgcg gtgcgaccgt aaaaaaaacc cacaaacaca ccaaagcatc
     3721 taaagccaag gccaaatctg ccgaaggcaa atgcggcgaa ggcaaatgcg gttctaaata
     3781 atcccacccc ttcaaaccaa gccgcgtttt tcagtaaaat gcggcttttt ttaacggcaa
     3841 acaaagattt tttaacaagc acatcattct tttgtgccat ccgaaccggg taaaaatatg
     3901 attcaacacg caggcttggg ctaccgccgc gacttggcgg aagactttct ctcgctttcc
     3961 gaaaacagcc cgatatgctt tatcgaagcc gcaccggaaa actggctgaa aatgggcggc
     4021 tgggcgcgca aacagtttga ccgtgtggcg gaacggctgc cgctggcgtt gcacggatta
     4081 tctatgtcgc tgggcgggca agcaccgctg gatactgatt tgatagacgg catcaaagaa
     4141 atgatgcgcc gttacgattg cacgtttttc tccgaccatt tgagctactg ccacgacggc
     4201 ggtcatcttt acgatttgtt gccgctgccc tttatcgagg aaatggtgca tcatacggcg
     4261 cggcgtatcc gcgaagtgca agaccgtttg ggctgccgca tcgccgtgga aaacacgtcc
     4321 tactatctgc attccccgct tgccgagatg aacgaggtcg agttcctcaa cgcggttgca
     4381 cgtgaggccg attgcggcat tcatctggac gtgaacaata tctacgtcaa cgccgtcaat
     4441 cacggcctgt tgtcgccgga ggcttttttg aaaaacgttg acgcagatcg cgtgtgctat
     4501 atccatattg ccggacatga cgtggaaacg ccggaattgt tgattgatac acatggcgcg
     4561 gcagttttgc cgactgtttg ggacttgctc gaacttgcct atgccaaact gccgacgatt
     4621 ccgcccaccc tgttggaacg cgattttaat ttcccgcctt ttgccgaact cgaagccgaa
     4681 gtcgccaaaa tcgccgatta tcaaacgcgt gccggaaagg aatgccgccg tgcagcctga
     4741 aacctccgcc caataccagc accgtttcgc ccaagccata cgcgggggcg aagccgcaga
     4801 cggtctgccg caagaccgac tgaacgtcta tatccgcctg atacgcaaca atatccacag
     4861 ctttatcgac cgctgctata ccgaaacgcg gcaatacttt gacagcgaag aatggagccg
     4921 tctgaaagaa ggtttcgtcc gcgacgcgca cgcccaaacg ccctattttc aagaaatccc
     4981 cggcgagttc ctacaatatt gccaaagcct gccgctttca gacggcattt tggcactgat
     5041 ggattttgaa tatacccaat tgctggcaga agttgctcaa attccggata ttcccgacat
     5101 tcattattca aatgacagca aatacacacc ctcccctgcg gcctttatcc ggcaatatcg
     5161 atatgatgtt accgatgatt tgcatgaagc ggaaacagcc ttgttaatat ggcgaaacgc
     5221 cgaagatgat gtgatgtacc aaacattgga cggcttcgat atgatgctgc tagaaataat
     5281 gggttcctcc gcgctttcgt ttgacaccct cgcccaaacc cttgtcgaat ttatgcctga
     5341 ggacgataat tggaaaaata ttttgcttgg gaaatggtca ggctggactg aacaaaggat
     5401 tatcatcccc tccttgtccg ccatatccga aaatatggaa ggcaattccc cgggccaaaa
     5461 ccatctatcc gcataaaatt accttgttcc cgatactatg ccgctacccg acctgaccga
     5521 tgccgaatta atagagtcgc gtaaactgct tctgcatttt gcgcggcttc agttgcccga
     5581 ccaccctgat ttggctgaag atttagtgca ggaaacattg ctgtccgcat acagcgcagg
     5641 cgacagtttt caaggcaggg cacttgtcaa cagctggctt tttgccatat tgaaaaacaa
     5701 aattattgac gcattacgtc aaatcggaag gcagaggaaa gtctttacca cactggatga
     5761 cgagctactg gatgaagcat ttgaaagcca tttttcccaa aacgggcatt ggacgccgga
     5821 agggcagccg caacattgga acactccgga aaaatcatta aacaacaacg aattccaaaa
     5881 aattctgcaa agctgcctat acaacctgcc tgaaaacacc gcacgggtat ttaccctgaa
     5941 ggaaatactc ggtttttcat ccgacgaaat acaacaaatg tgcggtatca gcacgtccaa
     6001 ctaccacacc attatgcacc gcgcccgaga atcattgcgc caatgcctgc aaatcaaatg
     6061 gttcaaccaa gaaaacccga agtaaacgtt atgaaaaaat gccgcgatat cgccctgctt
     6121 ctttccaaac atcaggaccg ggaaaccacc ccgggcgaga agatttccat atacacacac
     6181 ctgctgttct gtccgtattg ccgtgaatat aaaagacaac ttcaaaccat caaaagatca
     6241 ctggcaaaaa caaccagaac ttcaaaataa atgccgtctg aaaaggcttc agacggcata
     6301 agctgacgga aacaaatcaa accgatttac tgttatcggc agttcatcca taatacacgc
     6361 ttcaaaagca gcatattcct ccatacggaa tatataaata cgcaaaacac gaaggctgca
     6421 tcaatttgct ttatttgcct tatttcacag acggcgctac ccctcccgcc caacccgttc
     6481 tttctgaatg agcagatttc aatgattaag gaaaccctaa tgcgcccaat cttcctatct
     6541 ttcgttttac tccctatttt gataaccgcc tgcagcacac cggacaagtc tgcccgatgg
     6601 gaaaatatcg gcacaatctc aaacggcaat attcatacat atatcaataa agacagcgtg
     6661 agaaaaaacg gaaatctgat gattttccaa gataaaaaag ttgttaccaa tctaaaacaa
     6721 gaacgttttg ccaacacccc cgcatacaag actgccattg ccgagtggga aatccactgc
     6781 aacaacaaaa cataccgctt aagttcgcta cagttgtttg atacaaaaaa cacggaaatt
     6841 tccacacaaa actacacagc ctcttccctc cgcccgatga gcatcctgtc cgggacatta
     6901 accgaaaaac aatatgaaac cgtatgcgga aaaaaactct gattgcaact tatacacaaa
     6961 cttacccaca aaccttatca taaaaatgcc gtctgaaata ctgaaatatc agcatttcag
     7021 acggcatttt gccattccct gaaaattatc cacaaagtta tccacattat tttttaaaac
     7081 cggcttccat ccgaa
//
LOCUS       DAXHNE010000103         7048 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.103, whole genome shotgun sequence.
ACCESSION   DAXHNE010000103 DAXHNE010000000
VERSION     DAXHNE010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7048
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.103"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            142..576
                     /locus_tag="ACFM4Z_001545"
     CDS             142..576
                     /locus_tag="ACFM4Z_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC6021557.1"
                     /translation="MNFALSVIMLTLASFLPVPPAGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(624..1967)
                     /locus_tag="ACFM4Z_001546"
     CDS             complement(624..1967)
                     /locus_tag="ACFM4Z_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFC6021558.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGVWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(1972..2625)
                     /locus_tag="ACFM4Z_001547"
     CDS             complement(1972..2625)
                     /locus_tag="ACFM4Z_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HFC6021559.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2776..3066
                     /gene="gatC"
                     /locus_tag="ACFM4Z_001548"
     CDS             2776..3066
                     /gene="gatC"
                     /locus_tag="ACFM4Z_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFC6021560.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAGAPEVRNRLYIVPQVIEE"
     gene            3129..4574
                     /gene="gatA"
                     /locus_tag="ACFM4Z_001549"
     CDS             3129..4574
                     /gene="gatA"
                     /locus_tag="ACFM4Z_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFC6021561.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQTGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFA
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4571..5503
                     /locus_tag="ACFM4Z_001550"
     CDS             4571..5503
                     /locus_tag="ACFM4Z_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFC6021562.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLADIESKKRSMYRKRYE
                     MLDNMVAEMKDSLKTEARGISDGIQTEKPPRRTA"
     gene            5546..6976
                     /gene="gatB"
                     /locus_tag="ACFM4Z_001551"
     CDS             5546..6976
                     /gene="gatB"
                     /locus_tag="ACFM4Z_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFC6021563.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINQKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRVDANVSVRPKGQEEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAHDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ESGQGKLTANWMNGELAAALNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPEATIAEIIEKHGLQQMTDTGEIEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
BASE COUNT         1834 a         2119 c         1759 g         1336 t
ORIGIN      
        1 ttttgggaat ctgttttacc ccaatatata aagcaccata ttaaggcgga gtgtcttccc
       61 cactttgacc cgaacccgga aaagacaccg cccaagccaa tcctgatgct gccccgacag
      121 ccaaccatta aggaaatcct aatgaacttt gctttatccg tcattatgtt gaccctcgcc
      181 tctttcctgc ccgtcccgcc tgccggagcc gccgtcttta cttggaagga cggcggcggc
      241 aacagctatt cggatgtacc gaaacagctt catcccgacc aaagccaaat cttaaacctg
      301 cggacgcgcc aaaccaaacc ggcggtcaaa cccgcccaag ccgacgcagg gaagcgcaca
      361 gacggcgcgg cacaggaaaa caatcccgac actgccgaga aaaaccggca gcttgaggaa
      421 gaaaaaaaaa gaattgccga aaccgaacgg cagaacaaag aagaaaactg ccggatttca
      481 aaaatgaacc tgaaggcggt gggaaattca aatgcaaaaa acaaggatga tttgattcgg
      541 aaatacaata acgccgtaaa caaatactgc cgttaatcgg ctctagcgca aacccgatgc
      601 cgtctgaagc ggcacggggt ttgtcatttc tgccagtagg ttttgacgtt gacgaactcg
      661 tacagcccga attcggacaa ttcgcgcccg taaccggaat ctttgactcc gccgaaaggc
      721 aggcgcaaat cgctgctggt atggcggttg ataaacaccg atcccgcctg tattttttcg
      781 gcaaaccgcc aagcgcgttc ggtatcggcg gtataaatgc aggcaccgag cccgaacggg
      841 gaatcattgg caagggcgat ggcatgttct tcgttttcgg cgcgcaaaat cagggcggcc
      901 ggcccgaata cttcttctct ccagacgcgg caggcaggat ttaccctgtc taaaaccgtc
      961 gcgggataaa accagcctcg cccttgtggg atttttccgc cggtcaggca taccgcgccg
     1021 tttgaaacgg catcttcaac ctgcccgtga accctgtccc gcaaatcttc gcggtgcagc
     1081 ggtgcaagcg tagtatcggg atgtttgggg tcgcccattt tcaatttagc gcattcggca
     1141 agaaacagcg tgataaaacg atcggctgcg gcttcggtta cgatgatgcg cttggcggcg
     1201 ttacacgatt gccccgcatc gcggaaacgg gaataacagg cttctgcggc ggcacgctcc
     1261 aaatcagcat cgggcatcac gataaaggcg ttgctaccgc cgagctccaa cacggttttc
     1321 ttaaggtttg cgcccgcgtg tgccgcaagg atgcgccccg tatgcgttga accggtaaac
     1381 gccattgcat cggtatcttc aaccgccttg agcgtgcccg cctcatccag ccacacgcct
     1441 gccagaggaa tgccgtctga agccaaatcg aacagtgcct gactgacgcg tgccacgctg
     1501 ggcgcgggtt tgacggcgca cgcgttgccc gcgcacatag cgggaacggc gaaacgcaat
     1561 acctgccaga cgggatagtt ccaaggcatg acggcaaaca ccacgcccaa aggctcgaag
     1621 cgcacctgac tcaaactcgc ctgcgtcgcg atggttttgt gggcaagcag ttcgggggca
     1681 aggcgggcgt aatagcgtat cagttcgata gacttgccga tttccgcacg gcattcgtgc
     1741 aagcagcgtc cgacttcctc acacaccatt tccgcaaaac gctctttctc cgcctccaaa
     1801 cggtcggcaa atttttgcag gcgcgcggca cgttcggtta cgcccagttg cgcgaacgcc
     1861 ccgccgcgca ttttcaaatc cgccagccgc cgttcaaact ccgcataatc ttgagcgggg
     1921 cggcggtaaa gcgtttcgcc cgtaaataca ttgacactgt gaaacatcga atcaacctgc
     1981 cagttgcggg aatatcgttt tcagtcccga cacaataatc tccaccgata ccgccgccag
     2041 catcataccc ataatgcggt ttaaaatcgt cagccccgtc gcgcccagca ggcggctgac
     2101 cttcccggca acgattaaaa tggcataaca aatcgcactg accaccaaac cggccgcgat
     2161 aatcaacgcg atgtcgccgt atgttttagc cgccgaagcg taaataatca cggtcgaaat
     2221 accgcccggg ccgatggtga tcggtatggc gatgggcacg acggcaatcg ctccggcatt
     2281 gcgggcgggg cgcgcctgcc ccgtttccgg ctgcgcgccg agattctgct tggcgggatt
     2341 gtcgttgccg ttcatcatcg aaatggcgat cagcagcacc aaaatcccgc cgccgacctg
     2401 aaacgaaccg acgctgatgc ccaaaacctt cagcagcgta ccgccgatca gcgcaaatac
     2461 cgcaatcacg gcaaacacgg caacggcggc cgtccgcgcg accttcctgc gctccttcgt
     2521 gctgtgcccg ttggtcaggt caaggtaaag cgacaacgcg ctaaacggat taatcagcac
     2581 caaaaaagcc acaatcagct tgccgatttc catgcccaat cccattattt ccccctcctt
     2641 caaacccgtg cggcaggcat ccgatgctgc aaattgccgc cgcaacggat ttttccgtta
     2701 taattaaaaa ttcaagcaat acgccccatc atacccgaac gacggtatct ttaccatcag
     2761 acaaggatgc ttttcatggc actgacactt gccgacgtag acaaaatcgc ccgactctcc
     2821 cgactgcacc tgactgcgga agaaaaagaa aaatcgcttc aagaattaaa cgacattttc
     2881 actatggtcg aacagatgca aaccattaac acagacggca tcgaaccgat ggcgcacccg
     2941 cacgaggccg ccctgcgcct gcgcgaagac gaagtaaccg aaaccgaccg cgccgccgaa
     3001 tatcaggcgg gtgctccgga agtacgcaac cgtctgtaca tcgtaccgca agttatcgaa
     3061 gaataatccg aatatgcttc agacggcatc agcaataccg cccgaagccc tttaaggatg
     3121 gaagatttat gacccaatac acattgaaac aggcaagcgt cctgttgcag tccaaacaga
     3181 tttccgccgt cgaactggca agcgcatacc ttgccgccat cgccgaaaaa aatcccgccc
     3241 tcaacggcta tatcaccatc gaccaagata aaacccttgc agaagcccgt gccgccgacg
     3301 aacgtatcgc gcagggcaac gcctccgcgc ttaccggcgt acccgtcgcc tacaaggata
     3361 ttttctgcca aaccggctgg cgcagcgcgt gcgcttccaa aatgctcgac aacttcatct
     3421 ccccctacac cgccaccgtc gtccaaaacc tgctcgacga aggtatggta acgctcggcc
     3481 gcaccaatat ggatgagttc gctatgggtt cgaccaatga aaactcattc tacggtgcag
     3541 ccaaaaaccc atggaatctt gagcacgtcc ccggcggttc gtcaggcggt tccgccgccg
     3601 tcgttgccgc gcgcctcgcc cctgccgcgc tcggttcgga caccggcggc tctatccgcc
     3661 aacccgcatc gcactgcggc attaccggca tcaaacccac atacggcacg gtttcccgct
     3721 tcggtatggt cgcctacgcc tccagcttcg atcaaaccgg cccgatggcg caaactgccg
     3781 aagactgcgc gattctgtta aacgcgatgg caggtttcga ccccaaagac tccaccagcc
     3841 tcgagcgcga aaaagaagac tacacccgcg atttgaacca accgctcaaa ggtttgaaaa
     3901 tcggcctgcc caaagaatat ttcggcgaag gcaacagcgc cgatgttctg acggcattgc
     3961 aaaacaccat tgatttgctg aaagcccaag gcgcggaatt gattgaagtt tccctgccgc
     4021 aaaccaagct gtccatcccc gcctactacg tcctcgcctc cgcagaagcc agcaccaacc
     4081 tttcacgtta cgacggcgta cgttacggac accgtgccgc ccaattcgcc gatttggaag
     4141 aaatgtacgg caaaacccgc gccgaaggtt tcggcagcga agtcaaacgc cgcatcatga
     4201 tcggcactta tgtactgtcg cacggctact acgatgccta ctatctcaaa gcccaaaaac
     4261 tgcgccgcct cgttgccgat gattttcaga cggcatttgc acggtgcgac ctcatcctcg
     4321 cgccgaccgc acccactgca gccccaaaaa tcggagcgga tgcttcgccg gttgaaacct
     4381 acttgagcga tatctacacc atcgccgtca acctcgccgg actgcccgca ttgaccctgc
     4441 ccgcaggctt cagcggcggc ggactgcccg tcggcgttca gcttgtcggc aactacttcg
     4501 ccgaagccaa aatcctcggt gcggcgcatc aaatccaact caacagcgat tggcacggca
     4561 aacgacccga atgaagcaga accgcacctt taccttcccc gattttcgca ccgtttacag
     4621 ctatgcgcct ttatatcggc tgcaacattt aaaatacaca ttgcgaaaat ttttcggaaa
     4681 aaaagaaatt tacgccttcg agcagtttgt caacgcctcc cctatccgtc aggggctgtt
     4741 cctccactgc ccgcaaaatg cctatccgct gctgcgcgaa tttgttgaca ggcgttttaa
     4801 ctgcaaacgc cgtttagatg cgatgacggc agattttctc atggcggaaa aactgttcgg
     4861 cacagacatc ctgcaccaaa tggaagacta ccgcttccat ttggtcttgg cgcacctttc
     4921 agacggcatc agcttgtggc tcaaccgcaa cgacaactgc gtcgaagaag gcgcgtggtc
     4981 tttatctttg cgcgacgaag caggcaaccg gctgtatatg gcgactttcg cctttgtcgg
     5041 cacacacctg ctgacagcct ccgtacaagg gccggcgggt gaagaagcca aagacaccgt
     5101 ccgccgcata accaaacaac tccacggctt gcgtccccaa caactgatgg taaccgccct
     5161 gcaatatttc gccgccgtac tcggcttgga cggcgcaatg ggcattgcac aaaaacatca
     5221 ggtcaaactg cgctggaaac ttaaaaagcg cgtcaaaatg aattacgacg cattctggca
     5281 ggaatacggc gcaagtttgg aacgggacgg ctactggcat ctcccccaaa cccccgcccg
     5341 caaagacctt gccgacatcg aaagcaaaaa gcgttcgatg taccgcaagc gttatgaaat
     5401 gctggacaat atggttgcag agatgaaaga cagtctgaaa acagaagcac gcggcatttc
     5461 agacggcatc caaacggaaa aaccgccccg ccggacagcc tgacgcgaag actatcgaat
     5521 tgatatttta gagaaagaag ctcttatgac ctgggaaacc gtaatcggct tggaaatcca
     5581 cgtccaattg aacaccaaat ccaaaatctt cagcggcgca tcgaccgcat tcggcgcaga
     5641 acccaacgcg cacgccagcg tagtggaatg cgcgctgccg ggcgttttgc ctgtgatgaa
     5701 ccgtgaagtc gttgaaaaag ccatcaaatt gggtttggct ttagatgcga aaatcaatca
     5761 gaaaaacgtg ttcgaccgca aaaactactt ctatcccgac ttaccaaaag gttatcaaat
     5821 cagccagttg gacttaccga ttgtcgaaca cggcaaattg gaaatcgtag tcggcgacga
     5881 tgtgaaaacc atcaacgtaa cccgtgcgca catggaagaa gacgcaggca agtccgtgca
     5941 tgaaggcttg aacggcgcaa ccggtatcga cctgaaccgc gccggcacgc cgctgttgga
     6001 agtggtatcc gaacctgaaa tgcgttccgc cgccgaagcc gttgcctacg ccaaggcctt
     6061 gcacagcttg gtaacctggc tggacatttg cgacggcaat atggcggaag gctcgttccg
     6121 cgtcgatgcc aacgtatccg tgcgcccgaa aggtcaagaa gagttcggca cgcgccgcga
     6181 gattaaaaac ctcaattcct tccgtttctt ggagcaggcg attaattacg aagcggaagc
     6241 gcaaatcgag attttggaag acggcggcaa agtacagcag gcaaccatgc tgtttgatcc
     6301 cgaaaaaggc gaaacccgcg taatgcgcct gaaagaagat gcgcacgact accgctactt
     6361 ccccgaccct gatttgctgc ccgttatcat ttcagacgcc caaatgcaaa aagccaaagc
     6421 agaaatgccc gagctgccga aagaaatggc agcgcgtttc gtggcggatt acggcgtgtc
     6481 cgaatacgac gcgcgcctgc tgaccgcaag ccgtgcgcag gctgcctatt ttgaagaagc
     6541 cgccaaagaa agcggacaag gcaagctgac tgccaactgg atgaacggcg aacttgccgc
     6601 cgcgctgaac aaagaaggca tggaacttgc cgacagcccg attaccgccc cgcgcctcgc
     6661 cgcgctggtt ggcaaaatcg ccgacggcac attaagcagc aagttagcga aaaaagcctt
     6721 tgaagccatg tgggcagaac ccgaagccac cattgccgaa atcattgaaa aacacggttt
     6781 gcaacagatg accgacaccg gcgagattga agccatggtg gacgaagtgc tggcaaacaa
     6841 cgccaaagcc gtggaacagt ttaaatccgg caacgaaaaa gccctgaatg cgattgtggg
     6901 acaagtgatg aaggccagca aaggcaaagc caaccccgcg caggttcaag agctgattaa
     6961 agccaaactg gcttaatccg ttatcacaca ggtcgtctga aagcaaagtt ccaacgaagg
     7021 taaaacagga aataagcttt cagacggc
//
LOCUS       DAXHNE010000104         7032 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.104, whole genome shotgun sequence.
ACCESSION   DAXHNE010000104 DAXHNE010000000
VERSION     DAXHNE010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.104"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(146..1942)
                     /locus_tag="ACFM4Z_001552"
     CDS             complement(146..1942)
                     /locus_tag="ACFM4Z_001552"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFC6021564.1"
                     /translation="MNTVSNYLSALREAMRAQGLDALVIPSADPHLSEYLPAHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLSGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLAAKNIRIEHPNDLLDQVWTSRPAIPAETVFIH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVATPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            1998..2339
                     /locus_tag="ACFM4Z_001553"
     CDS             1998..2339
                     /locus_tag="ACFM4Z_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021565.1"
                     /translation="MPSEKHNHLIKSPACCKTDGLIGWCVFAQKKRCKVVCCKILGIK
                     CRFIPFFGSNTENIVKFEHEVLIVCVGRYLDYVTIRAFIKISLPGGILFLKTVGFGFV
                     WGQSRPNWSNR"
     gene            2400..3653
                     /locus_tag="ACFM4Z_001554"
     CDS             2400..3653
                     /locus_tag="ACFM4Z_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC6021566.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3808..5319
                     /gene="lysS"
                     /locus_tag="ACFM4Z_001555"
     CDS             3808..5319
                     /gene="lysS"
                     /locus_tag="ACFM4Z_001555"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFC6021567.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSGIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDTEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(5396..5584)
                     /locus_tag="ACFM4Z_001556"
     CDS             complement(5396..5584)
                     /locus_tag="ACFM4Z_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFC6021568.1"
                     /translation="MDGWYDFRFQNVVYAPDRSPKISRMLSSILAGYAWDTENPFVAK
                     SEQRLSALSEWVGQLETE"
     gene            complement(5588..6466)
                     /locus_tag="ACFM4Z_001557"
     CDS             complement(5588..6466)
                     /locus_tag="ACFM4Z_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6021569.1"
                     /translation="MARRSKTFEEAAAEVEERFGHRGIKLVEFEGTAKPCVINCPKHG
                     NQTCSRYSNMFIGSSWGCPSCGNEQAAKAGIATLRKNHIALEMLKQAVTGMTKQERIT
                     TQAYNEMTKSVAGSNSIVLNDVQGDTTINNHHTHTHNRSVADGKALSMRLTPRPLLSD
                     RQAAAFARTGKLTGSFDLFASVVAPSQYTFAVAMPDTSMSPVIEKGDLLVVEPRMRPA
                     DEDIVLIELSDKRLVVAHLVIDIAGRMLIYQTGRPSEAFDLPEGSVIFGVVLEVKKRF
                     MSAAQARRRVDSDYRP"
     gene            complement(6496..>7032)
                     /locus_tag="ACFM4Z_001558"
     CDS             complement(6496..>7032)
                     /locus_tag="ACFM4Z_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6021570.1"
                     /translation="PFTDGNGRTGRILNSLLLIDKGLLDLPILYLSRYIIENRADYYR
                     LLLGVTERQDWESWIIYILDGVADTADWTVAKIDAIRRLFEQTRQHIRTHAQGIYTHE
                     LVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELSDIGVLQEIAIGRDKLFIHPRLME
                     LLRGEGNSFTSFQSLVKA"
BASE COUNT         1524 a         1812 c         1986 g         1710 t
ORIGIN      
        1 cgtactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta tatttgcttg
       61 cggcaagatg aaaacggtgc gggatgtttt gggaaaccaa tgccgtctga agggctttca
      121 gacggcattt tttgcgccgt gctgtttaac gcgccagcgg ttcggtgcgt ttgatcagcc
      181 acgcttttgc cgcgccttcg gtcagcggct cgaggcggcg gcggacttcg gcgtggtagc
      241 ggttgaccca gtcgatttcg ccgtcggtca tgagggcggt gtccatcagg cgtgtatcga
      301 tggggcagag ggtcagggtt tcaaaacaga ggaagctgcc gaattcggtt tcttgagggg
      361 tggcgacggc ttggttggcg gcaaggtttt caatgcggat gccccatttt cccgggcggt
      421 agagtccggg ttcgatggag gtaaccatgc cttttttcat ggcggtttcg ggcgtggcgg
      481 gggcggcgaa ggcgatgcgc tgcgggcctt cgtggacgtt gaggaaatag cctacgccgt
      541 gtccggtgcc gtggccgtag tcgcattgcg cctgccacag gggtttgcgg caaatcgcat
      601 caatcagcgg cgaggggatg ttttcgggga acacggcttc ggcaagcgcg atatgggctt
      661 tgagaacgag ggtgttgtcg cgtttttgtt cggcggtggg tgtgccgaca gggacgacgc
      721 gggtgatgtc ggtcgtgccg cctttgtatt gcgcgccgga gtcgatgagc aaaagcccgt
      781 tgccgctgat ggtgctgtgg ctttcgggcg ttgcgctgta atgcggcagt gctccgttgg
      841 cgttgaagcc cgcgatggtg tcgaaactca atgaaatgaa gcccgggcgc acgctgcggt
      901 ggcgataaag catggtgtcc acgtcgattt cggtcaggct gccgccgttg ccgatgatgt
      961 cttcaaactc ggcgaagaaa ccgcacaacg ccgcgccgtc gtgttccatc gcttcgcgga
     1021 tgcgggcgat gtcggcttcg gatttgcagg atttgaacag cgtggatggg ttgattccct
     1081 cgataaggcg cacgctttcg ggcaggcgca caagcgtgct gacggcggtt ttgttcggtt
     1141 cgataagcag cgcgccgccg atttgcgcga gtttgtcggc aacttgggca taaggttcga
     1201 ccgtgatgcc tgcggtttgc agcgcggcgg cggcttcggc gttcagacgg catcggtcgg
     1261 taaacaggac ggcgttgtct ttgccaatca gcaggaagga cacgaaaaca gggttgaaag
     1321 gtacgtcgct gccacgcagg ttggtcagcc aagcgatgtc gtcaagcgag gaaaccaagt
     1381 ggtaatccgc gcctttttcc gccatcacgg cgcgcacgcg ggcgagtttt tcggcggcgg
     1441 tttcggagac gtaggcgggg tcgtggatga acacggtttc ggcaggaatg gccgggcggc
     1501 ttgtccacac ttggtcaagc agatcgtttg ggtgttcgat gcggatattt ttggcggcga
     1561 gtgattgcgc caaagtgcgt ttgccggtga gcgagaccat atcggaaggg atgccgacgg
     1621 cggcgttttc gggcaggttt gccgcgagcc attcgttgta cggcggcact tgaccgcttt
     1681 tttgcagctc gatgccgctg ccggaaagct gtttggcggc ttgttcccaa tagcggctgt
     1741 ccacccatac acccgcttca tcggtggtca ggacaaatgt gccgaccgag ccggtaaagc
     1801 ccgacaattc gcggcgcgcc tgccagtgtg cgggcaggta ttcggacagg tgggggtcgg
     1861 cggaagggat gacgagtgcg tccaagcctt gcgccctcat ggcttcgcgc aatgcggaca
     1921 ggtaattcga tacggtattc ataacagttc ctccaatcgg gttttgcggc ttcagacggc
     1981 atggacggaa atctgaaatg ccgtctgaaa aacataacca tcttatcaaa tcgccagcct
     2041 gctgcaaaac ggacgggctg atcggatggt gcgtgtttgc gcaaaaaaag cgatgtaagg
     2101 ttgtttgttg taaaatttta ggcataaaat gtcgttttat tccttttttt ggaagtaata
     2161 cggaaaatat agtcaagttt gagcatgaag ttctaatagt gtgtgttggc aggtatttgg
     2221 attacgttac aatacgcgct ttcatcaaaa tttccctacc gggcggtatc ctgtttttga
     2281 agacggtagg atttgggttt gtttgggggc aatcccgccc aaattggagc aatagatagg
     2341 gctgtgtccg aatatgcggc tctgtgtgtt gaaacacagt taaaaatacg gaagacttta
     2401 tgtccgaaca acatatttcg acttggaaaa gtaaaatcaa cgcattgggt ccggggatca
     2461 tgatggcttc ggcggcggtc ggcggttcgc acctgattgc ctcgacgcag gcgggtgcgc
     2521 tttacggctg gcagatcgcg ctcatcatca tcctgaccaa cctcttcaaa tacccgtttt
     2581 tccgcttcag cgcgcattac acgctggaca cgggcaagag cctgattgaa ggttatgccg
     2641 agaaaagccg cgtttatttg tgggtattcc tgattttgtg catcctctcc gccacgatta
     2701 acgcgggcgc ggtcgccatt gtcaccgccg ccatcgtcaa aatggcgatt ccctcgctga
     2761 tgtttgatgc cggcacggtt gccgccttga ttatggcatc ctgcctgatt attttggtga
     2821 gcggacgtta ccgcgctttg gatcgcgttt ccaaaatcat catcgttact ttgagtatcg
     2881 ccacgcttgc cgccgccggc atcgctatgt cgcgcggtat gcagatgcag cccgatttta
     2941 tcgagccgac accgtggacg cttgccggtt tgggcttcct gatcgcgctg atgggctgga
     3001 tgcccgcgcc gattgaaatt tccgccatca attctttgtg ggtaaccgaa aaacaacgca
     3061 tcaatccttc cgaataccgc gacgggattt ttgattttaa cgtcggttat atcgccagtg
     3121 cggttttggc tttggttttc cttgcactgg gtgcgtttgt gcaatacggc aacggcgaag
     3181 cagtgcagat ggcgggcggc aaatatatcg ggcaattgat caatatgtac gccgttacca
     3241 tcggcggctg gtcgcgcccg ctggtggcgt ttatcgcgtt tgcctgtatg tacggcacga
     3301 cgattaccgt tgtggacggc tatgcccgtg ccattgccga acccgtgcgc ctgctgcgcg
     3361 gaaaagacaa aacgggcaac gccgaattct ttgcctggaa tatttgggtg gcgggcagcg
     3421 gtttggcggt gattttttgg tttgacggcg taatggcgaa tctgctcaaa tttgcgatga
     3481 ttgctgcttt tgtgtccgcc cctgtgtttg cctggctgaa ttaccgtttg gtcaaaggtg
     3541 atgaaaaaca caaactcaca tcaggtatga atgcccttgc attggcaggc ttgatttatc
     3601 tgaccggttt taccgttttg ttcttattga atttggcggg aatgttcaaa tgataacgat
     3661 gccgtctgaa accgcaaacc gctttcagac ggcattgccg cgtttatgga aaaaaatgcc
     3721 gacatcgcgt aaaatatgcg caaattttgt gattcggtca ggtcgtctga aacagattgc
     3781 gcctgattta ttttttcgga aaaccctatg agcgaacaaa accatccgca aaccgagccg
     3841 cagttggacg aaaaccaaat catcgccctg cgccgcgaaa aactgcacaa catccgccaa
     3901 cagcgcaacg cctatcccaa cgacttcaaa cgcgacagct tcgccgccga tttgcacgcc
     3961 caatacggcg aaatcggcaa agaagaactc gatccgcaag gcattcccgt caaagtggcc
     4021 ggccgcatga tgctgaagcg tcaaatgggc aaggcgagct ttgccactat tcaagacgtg
     4081 tccggacaaa tccagcttta tctgaacaac aaaggcgtga gccaagaagt tttggacgac
     4141 ttcaaccatt gggatttggg cgacatcgtc ggcgcggaag gcactttgtt caaaaccaac
     4201 cacggcgaac tgaccgtacg cgtgtccggc atccgcctgc tgtccaaatc cctgcgtccg
     4261 ctgcccgaca aacacaaagg tttgagcgat caggaaacca aataccgcca acgctatgtc
     4321 gatttgattg ccaacgaaga atcgcgcaat acctttatca aacgcagcca aatcatccaa
     4381 tccgtgcgta attttatggt gggcgagcat tatctcgaag tcgaaacccc gatgatgcac
     4441 ccgattcccg gcggcgcgac ggcaaaaccc ttcgttactc atcacaatgc cttagatatt
     4501 ccgctttacc tgcgtatcgc gcctgagctg tatttgaaac gcctggttgt cggcggcttg
     4561 gaacgcgtgt ttgaaatcaa ccgcagcttc cgcaacgaag gcatgtccgt gcgccacaac
     4621 cccgaattta cgatgattga attttacgaa gccttctccg actacgaacg tatgatgcag
     4681 atggcggaag acatcatccg caacgcatcg cgcacagtaa acggtacggc aaacatcact
     4741 tacaatggca aagaagtcga tttggaaagc ccgttcgagc gtctgaccat cctcgaagcc
     4801 atcaaaaaat acaatccgca ctacaccgac gagcagttga acgatacgga atggctgaaa
     4861 aaagaaatcg tcaaacacgg tgaaagcctg ccgccgtccc cgggcatcgg cagcctgcaa
     4921 ctcgcgctgt ttgaaggttg cgccgagggc aagctgtgga acccgacctt tattgtcgat
     4981 tacccggtcg aagtttcccc gttggcacgc gcttcggata cgaaacaagg tctgaccgaa
     5041 cgcttcgagc ttttcgttgt cggccgcgaa ctggcaaacg gctattccga gttgaacgac
     5101 cccgaagacc aagccgaacg cttcaaagcg caagtggcgc aaaaagacgc gggcgacgac
     5161 gaagcgatgc actacgatgc cgactacatc cgcgcgatgg aattcggttt gccgccgacc
     5221 ggcggttgcg gcatcggcat cgaccgctta gtgatgctgc tgaccgattc gcaaaccatc
     5281 cgcgatgtga ttctgtttcc gcagatgcgc cccgagtaat cataaaaaca gttgaaatag
     5341 aaatgccgtc tgaacccgat tggattcaga cggcattttg tatggcggta cggatttatt
     5401 cggtttccaa ctgaccgacc cattcggaca aggccgacag gcgttgttcg gatttcgcca
     5461 cgaacgggtt ttcggtatcc cacgcgtagc ctgccaaaat cgaagaaagc atacggctga
     5521 ttttcgggct gcggtcgggc gcgtacacga cgttttggaa gcggaaatcg taccaaccgt
     5581 ccacatatca ggggcggtaa tccgaatcaa cacgccttct cgcctgtgcg gcggacataa
     5641 accgtttttt gacctccagc accacaccga aaatcacgct gccttcgggc aggtcaaagg
     5701 cttcagacgg cctgcccgtc tgataaatca gcatcctgcc cgcaatatcg ataacaaggt
     5761 gcgcgacgac cagccgcttg tcggacagtt caatcagcac gatgtcttcg tccgcagggc
     5821 gcatacgcgg ctcgaccacc agcaaatccc ctttttcgat aaccggcgac atggacgtgt
     5881 cgggcatggc aacggcaaac gtatattgtg aaggggcgac caccgatgca aacaagtcga
     5941 aactgcccgt gagtttgcct gtacgggcga aagccgccgc ctgacggtct gacaacaaag
     6001 gacggggtgt gagcctcatc gacagtgctt tgccgtcggc aacgctgcgg ttgtgcgtat
     6061 gcgtatgatg gttgttgatg gtcgtatcgc cttggacatc gttaaggact atgctgtttg
     6121 aacctgccac ggatttggtc atctcattgt aggcttgcgt cgtgatgcgc tcttgcttgg
     6181 tcatacctgt tacagcctgt ttcagcattt ctaacgctat gtgattcttc ctaagggtcg
     6241 ctataccggc ttttgcagct tgctcattac cacaagaggg gcaaccccag ctacttccta
     6301 tgaacatatt ggagtacctc gaacaggttt ggtttccatg tttagggcag ttgattacac
     6361 acggcttggc tgtaccctca aactcgacca acttaatgcc acgatgaccg aaacgttcct
     6421 caacctcagc agcagcttct tcaaatgttt ttgaccttct tgccatattg acctccggat
     6481 tattgattat tttggctatg ctttaacgag ggattggaat gaggtaaagc tgttgccctc
     6541 tccccgcaat agttccatta ggcgcggatg aatgaatagt ttgtccctgc cgatggcgat
     6601 ttcttgcagc acacctatgt ctgagagctc tttcagatac ttagaagccg tctgccgttt
     6661 ggctatccct gccgcttcta ggttggcaat gcgtgtatat ggctgttcaa acagaagatt
     6721 taccagttcg tgcgtgtaga ttccttgtgc gtgtgtccgt atgtgttgcc gtgtctgctc
     6781 aaacaggcgg cgtatcgcat ctattttcgc taccgtccaa tcggcggtat cggctacgcc
     6841 gtctaagatg tagattatcc agctttccca gtcctgccgt tcggttacgc ctaaaagcag
     6901 gcggtaatag tccgccctgt tttcgataat atagcggctc aaatacaaaa taggcaaatc
     6961 caaaagccct ttatcaatca atagcaggct gttcaatatg cgccccgtcc gcccgttgcc
     7021 gtccgtaaac gg
//
LOCUS       DAXHNE010000105         7006 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.105, whole genome shotgun sequence.
ACCESSION   DAXHNE010000105 DAXHNE010000000
VERSION     DAXHNE010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.105"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(75..1496)
                     /locus_tag="ACFM4Z_001559"
     CDS             complement(75..1496)
                     /locus_tag="ACFM4Z_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HFC6021571.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGSLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(1855..2214)
                     /locus_tag="ACFM4Z_001560"
     CDS             complement(1855..2214)
                     /locus_tag="ACFM4Z_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HFC6021572.1"
                     /translation="MIRYLLIACGGISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2211..2816)
                     /locus_tag="ACFM4Z_001561"
     CDS             complement(2211..2816)
                     /locus_tag="ACFM4Z_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HFC6021573.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVIENGLEGIVQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2863..3345)
                     /locus_tag="ACFM4Z_001562"
     CDS             complement(2863..3345)
                     /locus_tag="ACFM4Z_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFC6021574.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3548..3645
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3856..4956
                     /gene="gcvT"
                     /locus_tag="ACFM4Z_001563"
     CDS             3856..4956
                     /gene="gcvT"
                     /locus_tag="ACFM4Z_001563"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFC6021575.1"
                     /translation="MTALKTTPFHQAHQDAGAKLVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVHNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQQA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLEKGGILRAHMEVLTDKGQGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLMRGKEVDVRVLKLPFVRNGQKQFD"
     gene            5118..5504
                     /gene="gcvH"
                     /locus_tag="ACFM4Z_001564"
     CDS             5118..5504
                     /gene="gcvH"
                     /locus_tag="ACFM4Z_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFC6021576.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDPYGAGW
                     FFKLKPANVADYDSLLTAEQYAGEVD"
     gene            5656..6903
                     /gene="hemA"
                     /locus_tag="ACFM4Z_001565"
     CDS             5656..6903
                     /gene="hemA"
                     /locus_tag="ACFM4Z_001565"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFC6021577.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPKAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYALDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
BASE COUNT         1782 a         1702 c         1968 g         1554 t
ORIGIN      
        1 tgtcggaagg ggggatattg atttgattat gccggaattt aaaatgccgt ctgaatgttc
       61 agacggcata gcgtttacag cagtttgaaa acgaaaaaga taagggtatg tacgatgaag
      121 acgggtgtca ggaaggcgac cgaccacatc atatagccga agaaagtcgg catcggtacg
      181 ccgcgctgtt cggcaatggc cttgaccatg aagttcggtg cgttgccgat gtaggtcagt
      241 gcgcccatga ataccgaacc catagaaacc gccagcagcg aatgaaacag ggaacccgtc
      301 atcaaggctt gggcatcgcc gcctgccata ttgaaaaaaa cgagataagt gggcgcgtta
      361 tccaagaatg ccgacaatat gccgctcatc caaaaataca tcacattaat cggatgacct
      421 gccgtatcgt gaaccagcga taccaccccg cccagcgcgc ctgcctcgcc tgctttcaga
      481 atgctcagga cgggaaagat ggtgatgaag atgccgagga agagtttgcc cacttcggcg
      541 atgggttcaa agttgaattc gttgcctgcg cggacttgtt tgggcgtgat tgccatagat
      601 acggcggtca atgcaatcag gatgacatcg cggacgaggt tttgcagggc gtaacggctg
      661 ccgaggattt caaatcccgg gtgttcgggt ttccaaaggc cggacattag aaccgcgccg
      721 accacgcccg aaagcaggag gaagttccat ttgccgaaga tggcgatttt ttcgggtttt
      781 tcctgttgtg ccggcgtatc ttgtgcaatg ctttcctgtt tgaagaaacg gttgtcgatg
      841 aaatagaagg cggtcaacag gacagcggtg ctgatcagga cgggggcgaa catatgtttg
      901 accgtccaca tgaaatctac gcctttgagg aagccgagga agagtggggg gtcgcccaaa
      961 ggggtcagac cgccgccgat gtttgcaacc aggaaaatga agaagatgac gatgtgcacg
     1021 cggcgggtac ggttttggtt ggctttcagc agcggacgaa tcatcagcat tgctgcgccg
     1081 gtcgttccca tgatagaggc aagtgccgta ccgacggcaa gcagggcggt gttgagcttg
     1141 ggtgtgccgt tcaagtcgcc ccaaaccaaa atgccgcctg aaatggtgta cagggcaagc
     1201 agcagcagga tgaaagggat gtattcttca acgagtgcgt gtgcgacggt atggataccg
     1261 gcggacgcgc caaaaaccaa actgaacggg atgaggaaga gcaatgtcca aaaggcggta
     1321 attttgccgt aatggtgatg ccaggtatgc gaaaaaaaca agggacccaa tgcgatagac
     1381 agcaaaatca gggcaaaggg caggctccac agcaggttta ggtttgcgcc gtccaaatct
     1441 gcggcgtaaa ccgatgcggg gaaaagcatt agtgaaaaca ggggtaggtg gcgcatcgtg
     1501 tttcctcgat tcaagcactg ccttgcgcgg cgcgcgggag tgatacaggc accgtgccgc
     1561 ccggacatag gcggactgtg ctgcctgttt gtttttgaaa aaagttttcc gattgattgt
     1621 aaagtaaata atcaggataa accagtttcc caaaccggaa ggcggcggga aggcggattg
     1681 ccgtgcttgg gaatatatgt tctttttgat aaataatttt atttaaaaca aataaatata
     1741 tgtctttaat aaataatcta tcgaaaacga aaaatgaatt tattttaaca tatatttgca
     1801 atgaaacagg tttgccccac ccgtttgttt gcccttatcc ctttcagacg gcattcaaga
     1861 ttcgggcctg cgccacatcc atatggcgac aagggaacaa aaaaccgatg aaaccgcccc
     1921 gacccaccag cgttggggaa actgccaaaa cattatcagg caggatgcgg tcatcatact
     1981 gatggcgaat attttggctt tgcgaggcac tgcgccgttt tgttcccagt tatgaaccat
     2041 cggaccgaaa tagcggtgcc ggtgcagcca gcggtgaaag cgcggggatg cctttgccca
     2101 gcaggcggcg gagagcagta cgaacggcgt ggtcggcaac agcggcaaaa aaatgccgat
     2161 tatccccaac agcagggaga tgccgccgca ggcaattaaa agataacgta tcattttgaa
     2221 atatttttct tattgtgcgg ataagggcag gatgtgatac cgagttttgc ccagccttca
     2281 tgtcccattt tttccagcag ggcgatattg cgttcgaata ttgccgaagc gtcgggaaag
     2341 gcttgtgcgg ctttggcaat gctgtcttcg cggatgaggt gcagcgtcgg atagggagaa
     2401 cggttggtgt agttgccgat gtcgtcgggt tcggtattct gaaagcggaa ttggggatgg
     2461 aacggtgcga tttggacgat gccttccaag ccgttttcga taacggcggc atcggcaatg
     2521 tcgagcatat cgttgaatac gtcgaaatcg gggaataggg tcgggtgaac cagcagggtg
     2581 gtttccagtt cggtggcggg tgtattgccc agtcgctgca gttcttcgtc caagtcttcc
     2641 aaaaaactgt caaggtgttt ggcttcgctg attgcgatgc ggacaaggtt tttaacgtgg
     2701 ggggctttgg caaagggaca caggttcaga ccgatgacgg ctttttccaa ccattgtccg
     2761 gtgtgttcgg caacagcatc tttattttcg gaagtattga tattcattat tgtcatgtaa
     2821 atgtgtttgc agattgcacg tgcgggaaaa tcgggaaggg cactattcct tcagcaggtg
     2881 gttgagcggc agggaggtgg tgtgtttgat ttcttttaaa acaaagctcg attgcgcatc
     2941 ttgtacgccg tggtgggaca ggagcgtatc caaaacaaaa tgggaaaacg cgttcatatc
     3001 ggtaaaaaac gcctgaagca ggtagtcggt ttcccctgtc agggcgaagc agctcaagac
     3061 ttccggccat tttcggaccg atgcggcaaa gtcttcccgc gcgtcttttg ctttgcggat
     3121 ggaaacgcgg ataaatgcct gaagccccag gttgacggat tccggagaca gcagcgcggc
     3181 atattggcgg acgatgccgg catcttccaa ctgcttcaga cggcgcaggc acggagaagg
     3241 cgaaagtgcg acacgttcgg acagttcgac attggtcagc ctgccgtttt cctggagaac
     3301 ctgtaagatt ttaatatcgg ttttgtctaa agtgagttgg ggcatatttg cgttccgttt
     3361 taaggaattc ggattgtctg tccgtatgtt tgcggcaatc cgcacagatg gagaccatat
     3421 taacatataa aaagttatac cgtcatccgg gacaaatttt gttttcggaa aatcatgtga
     3481 aaacagaggc ggtcggtttg catctcttta agacggcttg cccaaaccgc cgattcaaga
     3541 cataatcggg aaatgtgcag gagagtgtta cacccaacta caatgtaacc accgaaggcg
     3601 cagacaccct taaatcgctc aggtatcagg gactgcacat tgaaacaaac aatctggaga
     3661 gcggcgttgg aataacgtcc accgaagggg agaaggccgt ctgaaccacc attcagacaa
     3721 ccgcgcaaag cagtgagcag actggtttgc catcatgcgg atacagccga aaatctcagg
     3781 ttcaaggaca gatagggtca tccgcgcaca ggtgcgcggg cggcatctga acaaaaaatc
     3841 cggagaaact tgagaatgac tgctctgaaa accaccccat ttcatcaagc ccatcaagat
     3901 gcaggcgcga agctggtcga ttttgccggc tgggagctgc ccatccatta tggttcacaa
     3961 atcgccgaac acgaagccgt gcgcaccgac gccggtatgt ttgacgtatc ccatatgctc
     4021 gttaccgacg tagcaggcgc aaatgccaaa gcctttttcc gcaaattgat tgccaacgat
     4081 gtcgccaagc tcgcttttgt cggcaaagcc ctttattccg ctttgctcaa cgacaacggc
     4141 ggtgtgattg acgacttaat cgtttaccgc accaatgaag ccgaaaccca ataccgcatc
     4201 gtgtccaacg gcgcgacccg cgaaaaagac acggcgcaat tccacaaagt cggacaagag
     4261 ttcggcgtcg ccttcaatcc gcgctacgac ctcggcatgc tcgccgtaca aggccctaaa
     4321 gccattgaaa aactcctgac cgtcaaaccc gaatgggcag atgtcgtcca taacctcaaa
     4381 ccgttccaag gcgcggattt gggcaacgac tggtttgtcg cccgcaccgg ctacaccggc
     4441 gaagacggcg tcgaagtcat cctgcccggc accgaagccg tcgcattctt caaagccctg
     4501 caacaagccg gcgtacagcc ctgcggcctc ggcgcgcgcg acaccctgcg catggaagcc
     4561 ggcatgaacc tctacggcaa cgatatggac gacgacacca gcccgctcga agcaggtatg
     4621 ggttggaccg ttgacttgaa agacgaaagc cgcgacttcg tcggcaaagc cgccttgctg
     4681 gcattgaaag aaaaaggcgt tgccgtcaaa caggtcggcc tgttgctcga aaaaggcggc
     4741 atcctgcgcg cgcatatgga agtgttgacc gacaaaggcc aaggcgaaac caccagcggc
     4801 gtattctccc caagcctgaa acaatccatc gccatcgcgc gcgtaccgaa agattttgac
     4861 ggcgataccg ccaaagtgct gatgcgtggc aaagaagtgg acgtgcgtgt actgaagctg
     4921 ccgtttgtcc gcaacggaca gaaacagttt gattgatgcg gtttcagacg gcattttcat
     4981 ttcatatgcc gtctgaaagc aggttttaat tgttgtccga tacggacgtt tgtagaaagc
     5041 attgaacaag gcatctgtgg atattgattc atgcagatgc cgtctgaaaa taacccctat
     5101 caatggagta tcaaaccatg agcaacaaca tcccggccga actgaaatac gttgccagcc
     5161 atgaatggct gcgccttgaa gaagacggta cgattaccgt cggcattacc caccacgcgc
     5221 aagagctgtt gggcgacatc gtgttcgtcg agctgcccga agtcggcgcg aaccttgccg
     5281 ctgaagagca agccggtgtg gttgagtctg taaaagccgc gtccgacgtg tacgcaccga
     5341 ttgcaggcga agtcgttgcc gtcaacgaag atttgccaag cgctccggaa actgccaaca
     5401 gcgatcctta cggtgcaggc tggttcttca aactcaaacc ggcaaacgtt gccgattacg
     5461 acagtctgct gactgccgaa caatacgcgg gcgaagtgga ttaaaccgcc cggctgcccg
     5521 acggcaaccg ccggacaaac ggaaactgca ccttcagacg gcatttttgc ggtcggaggt
     5581 gcagtttttt gtccgtgttt taaggaagca gttaggctat aataacggtc tatattcatc
     5641 tttaccgatt ttttcatgca acttaccgct gtcggactca atcatcaaac cgcaccttta
     5701 agcatacggg aaaagctggc gtttgccgcc gccgccctgc ctaaagccgt ccgcaatctt
     5761 gcccgaagca atgcggcaac ggaggcggta atcctttcta cctgcaaccg caccgagctt
     5821 tactgcgtcg gtgattcgga agaaatcatc cgatggcttg ccgattacca cagtttgccg
     5881 attgaagaaa tccgtccgta tctgtacgcg ctggatatgc aggagactgt gcgccatgct
     5941 ttccgcgtcg cctgcgggct ggattcgatg gtgttgggcg agccgcagat tttaggacag
     6001 attaaggatg ccgttagggt tgctcaagag caggaaagta tgggtaagaa actcaatgcc
     6061 ctgttccaaa aaaccttttc cgttgctaaa gaggtccgta ccgatactgc cgtcggcgaa
     6121 aactcggttt ccatggcttc cgcttccgtc aaattggcgg aacagatttt tcccgacatc
     6181 ggcgatttga atgtcttgtt tatcggcgca ggcgaaatga ttgagctggt tgccacttat
     6241 tttgccgcca aaagtccccg gctgatgacg gttgccaacc ggacgctggc gcgtgcacag
     6301 gagttgtgcg acaagctcgg tgtcaacgcc gaaccgtgcc tgctgtccga tctgcctgcc
     6361 attctgcacg attacgacgt agtggtttct tcaacggcaa gccagttgcc cattgtcggc
     6421 aaaggcatgg tggagcgtgc attgaaacaa aggcagagta tgccgttgtt catgcttgat
     6481 ttggcagtgc cgcgtgacat tgaagcggaa gtcggcgatt tgaatgatgc ctatctttat
     6541 acggtggacg atatggtcaa tatcgtccaa agcggcaagg aggcaaggca gaaggccgcc
     6601 gccgccgccg aaacgctggt gtccgagaaa gttgccgaat ttgtcaggca gcagcagggc
     6661 aggcagagtg tccccttgat taaggcgttg cgggacgagg gcgagaaagc gcgcaaacag
     6721 gtgttggaaa atgccatgaa acagcttgcc aaaggcgcaa cggcagaaga ggttttggaa
     6781 cggctgtccg tccaactgac caacaagctg ctgcattcgc cgacccaaac cttgaataag
     6841 gcgggggaag aagataaaga tttggttcat gccgtcgcgc agatttatca tttggacaaa
     6901 taacggtgcg ccgggaaaaa tgccgtctga agaggtttca gacggcatat ttttgtgctg
     6961 tctgacaaca tcgtgaaatc ccacattata tcgatgtaat cacaaa
//
LOCUS       DAXHNE010000106         6962 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.106, whole genome shotgun sequence.
ACCESSION   DAXHNE010000106 DAXHNE010000000
VERSION     DAXHNE010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.106"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(132..1478)
                     /gene="mnmE"
                     /locus_tag="ACFM4Z_001566"
     CDS             complement(132..1478)
                     /gene="mnmE"
                     /locus_tag="ACFM4Z_001566"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFC6021578.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAEALCGKTPKP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGVNDKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGEGEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1609..1764
                     /locus_tag="ACFM4Z_001567"
     CDS             1609..1764
                     /locus_tag="ACFM4Z_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021579.1"
                     /translation="MEKRNTYAFLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1761..2192
                     /locus_tag="ACFM4Z_001568"
     CDS             1761..2192
                     /locus_tag="ACFM4Z_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HFC6021580.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVFALACAFVIGRSRDQGKN"
     gene            2327..6628
                     /gene="app"
                     /locus_tag="ACFM4Z_001569"
     CDS             2327..6628
                     /gene="app"
                     /locus_tag="ACFM4Z_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HFC6021581.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNVGYRDVDFGAEGRNPDQHRFSYKIAKRNNYKNDKTHPYEKDYHN
                     PRLHKFVTEAAPIDMTSDMDGNKYTDRTKYPERVRIGSGWQFWRNDQDKGDQIAGAYH
                     YLTAGNTHNQGGAGGGWSSLSGDVRQAGNYGPIPIAGSSGDSGSPMFIYDAEKQKWLI
                     NGVLRTGNPWAGKENTFQLVRKSFFDEILEKDLRTSFYSPSGNGAYTITDKGDGNGIV
                     KQQTGRPSEVRIGLKDDKLPAEGKDDVYQYQGPNIYLPRLNNGGNLYFGDQKNGTVTL
                     STNINQGAGGLYFEGDFTVSPENNETWQGAGVHISEDSTVTWKVNGVANDRLSKIGKG
                     TLHVQAKGENQGSISVGDGKVILDQQADENNKKQAFSEIGLVSGRGTVQLNADNQFNP
                     DKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKESTVTITGNKDIATTGNN
                     NSLDSKKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLSGGTNLNGNITQTNGK
                     LFFSGRPTPHAYNHLNDHWSQKEGIPRGEIVWDNDWIDRTFKAENFLIKGGQAVVSRN
                     VAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEKTITDEKVIASLTNTD
                     ISGNVSLANHAHLNLTGLATLNGNLTAGGDTHYEVTHNATQNGNLSLVGNAQATFNQA
                     TLNGNTSASGNASFNLSDHAAQNGDLTLSGNAKANVSHSAFNGNVSLADKAVFHFENS
                     RLTGQISGGKDTALHLKDSEWTLPSGTELGNLNLDNATITLNSAYRHDAAGAQTGSVS
                     DTPRRRSRRSRRSLLSVTPPTSAESRFNTLTVNGKLNGQGTFRFMSELFGYRSDKLKL
                     EESSEGTYTLAVNNTGNEPASLEQLTVVEGKDNKPLSENLNFTLQNEHVDAGAWRYQL
                     IRKDGEFRLHNPVKEQELSDKLGKAEAKKQAEKDNAQSLDALIAAGRDAAEKTESVAE
                     PARQAGGENVGIMQAEEDTALAKQREAETRPATTAFPRARRARRDLPQPQPQPQPQPQ
                     RDLISRYANSGLSEFSATLNSVFAVQDELDRVFAEDRRNAVWTSGIRDTKHYRSQDFR
                     AYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTFDDGIGNSARLAHGAVFGQYGIGR
                     FDIGISAGAGFSSGSLSDGIGSKIRRRVLHYGIQARYRAGFGGFGIEPHIGATRYFVQ
                     KADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQHISITPYLSLSYTDAASGKVRTR
                     VNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAAAKGPQLEAQHSAGIKLGYRW"
BASE COUNT         1822 a         2051 c         1713 g         1376 t
ORIGIN      
        1 tgtactgtct gcggcttcgt tgccttgtcc tgatttttgt taatccacta taaaaccaaa
       61 cggatcggat tcccgttttt atgggataac ggaatgttca gccggacacc gccacgatgc
      121 tttcgatccg tttattttcc gatacaaaac ctcgaaaaaa tcacgccgag caggtcgtcc
      181 gccgtaaact cgcccgtgat ttcgccgcat gcgacctgcg ccaagcgcaa gtgttcggca
      241 aacagctcga tttgatggtt gccgcacaat gccgccagcg acaattcttc ctgcgctgct
      301 ttgagtgcgt tgacgtgccg cgtccgcgcc aaaaacagcc cttcgccctc gccctgccat
      361 ccggcctcgc gcaacaacgt ccgtttcagc gcgtccaagc cgtcgccggt tttcgccgac
      421 agcgcgatga cggtttccgc gcccgtaccg aacccgcctg ccgcgtgtgc gtgcaaatcg
      481 gatttgctgt ggatttcgat gcgtttcaac tccggcggca acgcgtccaa aatcgcccgt
      541 gtcttgtcgt tcacgccttc gcgcggatcg accaacacca gcgcgacatc ggcttcggat
      601 acggctttgc ggctgcgttc gatgccgatg cgttcgacca cgtcgtccgt ctcgcgcaaa
      661 cctgccgtat cgacaatatg caccggaacg ccgtcaatca ggatacgttc cctgaccgcg
      721 tcgcgcgtcg ttccggcaat atcggtaaca atcgccactt cgtcgcccgc cagcgcgttc
      781 aacaggctgg atttgccgac attcggcgcg ccgaccaata cgacattcag accttcgcgc
      841 aaaatcgcgc cctgctgcgc gttggcaagc acatcatcca cggcgcggcg caagccgtcc
      901 agtttgccgc gtgcgtctgc cgcttcgaga aaatcaatgt cttcctcggg aaaatctaac
      961 gtcgcttcga ccagcatccg caaggtaatc aagtcttcga ccagaccgtg tatccgccgc
     1021 gaaaaatcgc ctttgagcga gcgcaacgcc agacgcgccg ccgaacggct ggatgcgtca
     1081 atcaaatccg ccacgccttc cgcctgtgcc aagtccagtt tgtcgtttaa aaacgcacgc
     1141 ttcgtaaact cgcccggttc ggcaaggcgc gcgcccaatt ccaaacagcg gttcagcagc
     1201 atatccatca ctaccggccc gccgtgtccc tgaagctcga taacatcttc gcccgtaaaa
     1261 cttgccggtg cggcaaaaaa aagcaaaagc ccgctgtcga ttgcctgacc gtccgcgtcc
     1321 gtaaaatcag cataggttgc cacgcgcggc ttgggcgttt tcccacacaa agcctctgcc
     1381 atcggcagca ggtttttccc cgatatgcgt atcacgccca cgccgccgcg cccgggtgcg
     1441 gtagcgactg ccgcaatcgt tggaacgtta tccgacataa aacccccgaa aattcaaaac
     1501 agccgcgatt atagcaaatg ccgtctgaag tccgacggtt tggctttcag acggcataaa
     1561 accgcaaaaa tgcttgataa atccgtccgc ctgacctaat ataaccatat ggaaaaacga
     1621 aacacatacg ccttcctgct cggtataggc tcgctgctgg gtctgttcca tcccgcaaaa
     1681 accgccatcc gccccaatcc cgccgacgat ctcaaaaaca tcggcggcga ttttcaacgc
     1741 gccatagaga aagcgcgcaa atgaccgaaa acgcacagga caaggcgcgg caggctgtcg
     1801 aaaccgtcgt caaatccccg gagcttgtcg agcaaatcct gtccgacgag tacgtgcaaa
     1861 taatgatagc ccggcgtttc cattcgggac cgttgccgcc gccgtccgac ttggcgcaat
     1921 acaacgacat tatcagcaac ggggcagacc gcattatggc aatggcggaa aaagaacaag
     1981 ccgtccggca cgaaaccata cggcaagacc aaaccttcaa caggcgcggg caactgtacg
     2041 gcttcatcag cgtcatcctg atactgcttt ttgccgtctt cctcgtatgg agcggctacc
     2101 ccgcaaccgc cgcctccctt gccggcggca cagtgtttgc cttggcgtgt gctttcgtga
     2161 ttggaagaag ccgagaccaa ggcaaaaatt aatgacaaat cctagggcgt gcttcatatc
     2221 cgcccgaacg ccgaaccgca catattaggc acatcccgcg cgccgcccgg aagcggaagc
     2281 cgcgccctcc caaacaaacc cgaatcccgt cagataagga aaaataatga aaacaaccga
     2341 caaacggaca accgaaacac accgcaaagc cccgaaaacc ggccgcatcc gcttctcgcc
     2401 tgcttactta gccatatgcc tgtcgttcgg cattcttccc caagcctggg cgggacacac
     2461 ttatttcggc atcaactacc aatactatcg cgactttgcc gaaaataaag gcaagtttgc
     2521 agtcggggcg aaagatattg aggtttacaa caaaaaaggg gagttggtcg gcaaatcaat
     2581 gacaaaagcc ccgatgattg atttttctgt ggtgtcgcgt aacggcgtgg cggcattggt
     2641 gggcgatcaa tatattgtga gcgtggcaca taatgtaggt tatcgcgatg ttgattttgg
     2701 tgcagagggg cgaaatcccg atcagcaccg tttttcttat aagattgcaa aacggaataa
     2761 ttataaaaac gataaaacgc atccttatga gaaagactac cacaacccac gcttacataa
     2821 atttgttacg gaagccgccc cgatcgatat gacttctgat atggacggca acaaatatac
     2881 agataggacg aaatatcccg aacgcgtccg tatcggctcc gggtggcagt tttggcgaaa
     2941 cgatcaagac aaaggagacc aaattgccgg cgcatatcat tacctgacag caggcaatac
     3001 acacaatcaa ggcggagcag ggggcggctg gtcaagcctg agcggcgatg tgcgccaggc
     3061 aggcaattac ggtcccattc ctattgcggg ctcaagcggc gacagcggtt cgcccatgtt
     3121 tatttatgat gcggaaaaac aaaaatggtt gattaacggc gttttgagga caggtaaccc
     3181 ttgggcgggg aaagaaaata cattccaact ggtacgcaag tctttttttg atgaaatcct
     3241 tgaaaaagat ttacgtacat cgttttatag cccatcgggc aatggtgcat acaccattac
     3301 ggacaaaggc gacggcaacg gcattgtcaa acaacaaaca ggaagaccat ctgaagtccg
     3361 aatcggcttg aaagacgaca aattgcctgc cgaaggtaaa gacgatgttt accaatacca
     3421 aggtccaaat atatacctgc ctcgtttgaa taacggtgga aacctgtatt tcggagacca
     3481 aaaaaacggc actgttacct tgtccaccaa catcaaccaa ggcgcgggcg gtttgtattt
     3541 tgaaggtgac tttacggtct cgcctgaaaa taacgaaact tggcaaggcg cgggcgttca
     3601 tatcagtgaa gacagtaccg ttacttggaa agtcaatggc gtggcaaacg accgcctgtc
     3661 caaaatcggc aaaggcacgc tgcacgttca agccaaaggg gaaaaccaag gctcgatcag
     3721 cgtgggcgac ggtaaagtta ttttagatca acaagcagat gaaaataata aaaaacaagc
     3781 ctttagtgaa atcggcttgg tcagcggcag gggtacggtg caactgaatg ccgataatca
     3841 gttcaacccc gacaaactct atttcggctt tcgcggcgga cgtttggatt tgaacgggca
     3901 ttcgctttcg ttccaccgta ttcaaaatac cgatgaaggg gcgatgattg tcaaccacaa
     3961 tcaagacaaa gaatccaccg ttaccattac aggcaataaa gatattgcta caaccggcaa
     4021 taacaacagc ttggatagca aaaaagaaat tgcctacaac ggttggtttg gcgagaaaga
     4081 tacgaccaaa acgaacgggc ggctcaacct tgtttaccag cccgccgcag aagaccgcac
     4141 cctgctgctt tccggcggaa caaatttaaa cggcaacatc acccaaacaa acggcaaact
     4201 gtttttcagc ggcagaccga caccgcacgc ctacaatcat ttaaacgacc attggtcgca
     4261 aaaagagggc attcctcgcg gggaaatcgt gtgggacaac gactggatcg accgcacatt
     4321 taaagcggaa aatttcctga ttaaaggcgg acaggcggtg gtttcccgca atgttgccaa
     4381 agtagaaggc gattggcatt tgagcaatca cgcccaagca gttttcggtg tcgcaccgca
     4441 tcaaagccac acaatctgta cacgttcgga ctggacgggt ctgacaaatt gtgtcgaaaa
     4501 aaccattacc gacgaaaaag tgattgcttc attgaccaat accgacatca gcggcaatgt
     4561 cagccttgcc aatcacgctc atttaaatct cacagggctt gccacactca acggcaatct
     4621 gaccgcaggc ggagacacgc actatgaggt tacgcacaac gccacccaaa acggcaacct
     4681 tagcctcgtg ggcaatgccc aagcaacatt taatcaagcc acattaaacg gcaacacatc
     4741 ggcttcgggc aatgcttcat ttaatctaag cgaccacgcc gcacaaaacg gcgatctgac
     4801 gctttccggc aacgctaagg caaacgtaag ccattccgca ttcaacggca atgtctccct
     4861 agccgataag gcagtattcc attttgaaaa cagccgcctt accggacaaa tcagcggcgg
     4921 caaggatacg gcattacact taaaagacag cgaatggacg ctgccgtcag gcacggaatt
     4981 aggcaattta aaccttgaca acgccaccat tacactcaat tccgcctatc gccacgatgc
     5041 ggcaggggcg caaaccggca gtgtgtcaga cacgccgcgc cgccgttcgc gccgttcgcg
     5101 ccgttcccta ttatccgtta caccgccaac ttcggcagaa tcccgtttca acacgctgac
     5161 ggtaaacggc aaattgaacg gtcagggaac attccgcttt atgtcggaac tcttcggcta
     5221 ccgcagcgac aaattgaagc tggaggaaag ttccgaaggc acttacacct tggcggtcaa
     5281 caataccggc aacgaacctg caagcctcga acaattgacg gtagtggaag gaaaagacaa
     5341 caaaccgctg tccgaaaacc ttaatttcac cctgcaaaac gaacacgtcg atgccggcgc
     5401 gtggcgttac caactcatcc gcaaagacgg cgagttccgc ctgcataatc cggtcaaaga
     5461 acaagagctt tccgacaaac tcggcaaggc agaagccaaa aaacaggcgg aaaaagacaa
     5521 cgcgcaaagc cttgacgcgc tgattgcggc cgggcgcgat gccgccgaaa agacagaaag
     5581 cgttgccgaa ccggcccggc aggcaggcgg ggaaaatgtc ggcattatgc aggcggagga
     5641 agacaccgcc ttggcgaaac agcgcgaagc ggaaacccgg ccggctacca ccgcgttccc
     5701 ccgcgcccgc cgcgcccgcc gggatttgcc gcaaccgcaa ccccaaccgc aaccccaacc
     5761 gcagcgcgac ctgatcagcc gttatgccaa tagcggtttg agtgaatttt ccgccacgct
     5821 caacagcgtt ttcgccgtac aggacgaatt agaccgcgta tttgctgaag accgccgcaa
     5881 cgccgtttgg acaagcggca tccgggacac caaacactac cgttcgcaag atttccgcgc
     5941 ctaccgccaa caaaccgacc tgcgccaaat cggtatgcag aaaaacctcg gcagcgggcg
     6001 cgtcggcatc ctgttttcgc acaaccggac cgaaaacacc ttcgacgacg gcatcggcaa
     6061 ctcggcacgg cttgcccacg gcgccgtttt cgggcaatac ggcatcggca ggttcgacat
     6121 cggcatcagc gcgggcgcgg gttttagcag cggcagtctt tcagacggca tcggaagcaa
     6181 aatccgccgc cgcgtgctgc attacggcat tcaggcacga taccgcgccg gtttcggcgg
     6241 attcggcatc gaaccgcaca tcggcgcaac gcgctatttc gtccaaaaag cggattaccg
     6301 ctacgaaaac gtcaatatcg ccacccccgg ccttgcgttc aaccgctacc gcgcgggcat
     6361 taaggcagat tattcattca aaccggcgca acacatttcc atcacgcctt atttgagcct
     6421 gtcctatacc gatgccgctt cgggcaaagt ccgaacgcgc gtcaataccg ccgtattggc
     6481 tcaggatttc ggcaaaaccc gcagtgcgga atggggcgta aacgccgaaa tcaaaggttt
     6541 cacgctgtcc ctccacgctg ccgccgccaa aggcccgcaa ctggaagcgc aacacagcgc
     6601 gggcatcaaa ttaggctacc gctggtaaca ccggatatgc cgaaaggggt ttgacgatgc
     6661 cgccgtgcgc tgtcaaaccc tttttctgcc gccgccttgt gcctgctcca ttgtctgata
     6721 accgtcaaat tgatttttag cccatgtttg gtgggtcggg gaaatctata tcttcgtccg
     6781 tgccgaaata gtctgagacc tttgcaaaat tcctttccct cccgacagcc gaaatccaaa
     6841 cacaggtttt cggctgtttt cggctgtttc cgccccagat acctcctaat tctacccaaa
     6901 cgccccctta atcctccccg gatacccgat aatcaggcat ccgggctgcc ttttaggcgg
     6961 ca
//
LOCUS       DAXHNE010000107         6865 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.107, whole genome shotgun sequence.
ACCESSION   DAXHNE010000107 DAXHNE010000000
VERSION     DAXHNE010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.107"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(120..479)
                     /gene="rplT"
                     /locus_tag="ACFM4Z_001570"
     CDS             complement(120..479)
                     /gene="rplT"
                     /locus_tag="ACFM4Z_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFC6021582.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(492..689)
                     /gene="rpmI"
                     /locus_tag="ACFM4Z_001571"
     CDS             complement(492..689)
                     /gene="rpmI"
                     /locus_tag="ACFM4Z_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFC6021583.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(836..1357)
                     /gene="infC"
                     /locus_tag="ACFM4Z_001572"
     CDS             complement(836..1357)
                     /gene="infC"
                     /locus_tag="ACFM4Z_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFC6021584.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(1375..3288)
                     /gene="thrS"
                     /locus_tag="ACFM4Z_001573"
     CDS             complement(1375..3288)
                     /gene="thrS"
                     /locus_tag="ACFM4Z_001573"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFC6021585.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGVGLARAAVAGRVNGKL
                     VDACDPIVENSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVKLSLRPEQRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(3342..3418)
                     /locus_tag="ACFM4Z_001574"
     tRNA            complement(3342..3418)
                     /locus_tag="ACFM4Z_001574"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3382..3384),aa:Val,seq:gac)
     gene            3597..4712
                     /gene="tgt"
                     /locus_tag="ACFM4Z_001575"
     CDS             3597..4712
                     /gene="tgt"
                     /locus_tag="ACFM4Z_001575"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFC6021586.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQAKKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRTGEILGAQLNTIH
                     NLHFYQVIMAEMREAVEQGKFADWQARFHENRARGTD"
     gene            4722..5804
                     /gene="hemH"
                     /locus_tag="ACFM4Z_001576"
     CDS             4722..5804
                     /gene="hemH"
                     /locus_tag="ACFM4Z_001576"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFC6021587.1"
                     /translation="MPSEAASDGILPLYPTRTIFQKPIMLPFFPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAKRMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHHDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGSWR"
     gene            6100..6501
                     /locus_tag="ACFM4Z_001577"
     CDS             6100..6501
                     /locus_tag="ACFM4Z_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6021588.1"
                     /translation="MNTTRLPTALVLGCFCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKSKKN"
     gene            complement(6564..6854)
                     /locus_tag="ACFM4Z_001578"
     CDS             complement(6564..6854)
                     /locus_tag="ACFM4Z_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021589.1"
                     /translation="MAKYFTETGISVREHFDFFGEFVVSPAARSGDLALTYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVEGGRIDIGGFMLTAKEVDGGGNIGSMGLKVLR"
BASE COUNT         1743 a         1847 c         1598 g         1677 t
ORIGIN      
        1 actatatttt aacgcaaatt tatttaaaaa aatagacttt aaagaaagaa aatcaatcac
       61 atagaatttc tgcaaacaaa gaaaaaagga agccgcagct tccttttcaa ttttttggat
      121 taagcagcca aagcagcttt ggctttttca accaattgtg caaaagcggc tttatcgaac
      181 acggccaaat cagccaatac tttgcggtcg atttcgatag aggcgcgttt cagaccattc
      241 ataaatttgc tgtaagacaa cccgttttca cgcgcacctg cattgatacg gacaatccac
      301 aattgacgga attggcgttt gcgttggcgg cggtcgcggt atgcgtattg gccggctttc
      361 attaccgcct gcttggcaac gcggtaaacg tttttacgac ggccgcggta gcctttggct
      421 aacgcgaaga ttttttggtg acgggcacgg gcggtaacac cgcgttttac gcgtggcata
      481 ttctaaactc cttaagcgta gggtaacatt ttagcaacag aagccaaatc gcgatcgttt
      541 accatagagg taccgcgcag ttggcgtttg tttttggtgg tctttttagt caagatgtgg
      601 cgtttgaacg catgagcgcg tttcacaccg ccgttaccca gtactttaaa gcgttttttc
      661 gcgctagact tggttttcat tttaggcatg ggaaaactcc attcgttatc ggataaggca
      721 ttagggggtt ttaaaacatc agtttcaaac acttgaaccc acaataccac ggttttttgc
      781 cgcaattgaa aacttactcc gaagcggcaa tcggagtaag cggagaatta tagctttatt
      841 ttttcttcgg cgcaatcatc atcaccattt ggcgaccttc cattttggga aaggactcga
      901 tttgcgccac ttcagccaaa tcttctttta cacgttccaa aagttgcgcg ccgagttgct
      961 ggtgagccat ttcacggccg cggaaacgca atgtcacttt gactttatcg ccgtcggcaa
     1021 ggaagcggtt aatgttgcgc atcttgattt gataatcgcc ctcatccgta cccggacgga
     1081 atttgatttc cttgatttgc acctgctttt ggtttttctt ggcttcgtcg cgtttcttgg
     1141 cctgctggta tttgtattta ccgtaatcca tcagtttaca cacaggcggt ttagcagttg
     1201 gggaaatctc taccaaatcg acatcctgcc cttcggccat agccaaagct tcacgaactg
     1261 aaacgacacc aagctgttcg cctgactcac tgattaaacg cacttctttg gcggtaattt
     1321 cgccgttgat tcgtgcttcg cgttcttgag cgatgatgaa tactcctata aaaattaatg
     1381 attgacgagg gcatcagtga tttcttgctg caattgcgca atgaaatcat ccaaatccaa
     1441 agaacccaaa tcttctgctt tgcggcgtac cgccactttg ttttcctgct tttctttatc
     1501 gccgacaacg atttgataag gaaaacggta ttggctgttg tcgcggattt tgtaaccgat
     1561 tttttcgtta cgcaaatcca actcggcgcg gaatcctgcc gcctgcaatt tggcagccac
     1621 ttcacggcag taatccgctt gattttcggt gatattcata atcaccattt gaaccggcgc
     1681 cagccataac gggaaagagc ctgcatggtt ctcaatcaga atgccgataa accgctccaa
     1741 agaacccaaa atggcgcggt gcaacattac agggcgagcg cggtcattgt tttcggttac
     1801 atattctgca tccaagcgtt ccggtaagac gaaatctagt tgcaatgtac cgcattgcca
     1861 agaacgaccc aaggcatctt tgacgtgata ttcgatttta ggcccgtaaa acgcaccttc
     1921 gcccggcaat tcgccccatt ccacgccgca ggcagtcaat gcctcgcgca aaccctgctc
     1981 tgccttatcc cacacgtcat ctgaacctgc acgttgttca gggcgaagag aaagcttgac
     2041 ggatacatca tggaaaccga actgtttgta gatgcgaacc aacaattcat tgaatgcgcg
     2101 cgcttcgctg acgatttggt cttcggtaca gaaaatatgc gcatcatctt gcacaaaacc
     2161 gcgaacacgc atcagaccat gcaatgcacc gcttggttca ttgcggtggc aagaaccgaa
     2221 ctcggccaaa cgcatcggca aatcgcgata tgagcgcaaa ccgttattaa aaatttgcac
     2281 atgacccgga cagttcatcg gtttcaccgc gtattcgcgt ttttctgaac tggtcacgaa
     2341 catattgtct ttgtagttgt cccaatggcc ggatttttcc caaaaggttt tatccataat
     2401 ttgaggcgtt ttgacctctt tataaccggc ggcgttcagc tctttacgca tatgctgttc
     2461 aatcacttgc cacaaagccc agcctttagg atgccaaaac accatgcccg gcgcttcgtc
     2521 ttgcagatgg aacagatcca attgcttgcc aagtttgcgg tggtcgcgct tttcggcttc
     2581 ttcgatacgt tgaatatagg ctttcaactc gtcttttgtc gcccaagccg taccgtatat
     2641 gcgttgcagc atttcattgt tgctgtcgcc gcgccagtat gcgcccgcca acttggtcag
     2701 tttgaagttt ttcaggaaac gggtattcgg aacgtgcggt ccgcggcaca tatcgacata
     2761 ttcctgatga tgatacatcc ccatcgcttc cacttcgggc atatcgtcaa tcaggcgcag
     2821 tttgtattct tcgccgcgct cttgaaaaat tttaatcgcc tccgcacgcg gagtcatgat
     2881 tttgaccaca tcatagtctt gggcaatcaa ttctttcata cgcgcttcaa tggcggcaac
     2941 atcttccggt gtaaacggtt tttccgtggc gatgtcgtaa taaaagccct cttcaatgac
     3001 ggggccgata accatttttg cattaggata gagttgcttg acggcatgcc cgacaagatg
     3061 ggcgcaggaa tggcggatga tttcgatgcc ttcctgatct ttcggagtaa tgatttgaac
     3121 agcagaattt tcaacaatcg ggtcgcacgc atcgaccaat ttgccgttta ccctgcctgc
     3181 caccgccgcc ctcgccaaac cgacaccgat agacgcagca atttgagcca cggtaacggg
     3241 ggattcgtat tggcggactg agccgtccgg caaggtaatg ttcaacatca aaaagctcca
     3301 aaacattaaa aaataacggc ataaatgccg ttatgggaat ttggtaggca cgattggatt
     3361 cgaaccaacg acccccacca tgtcaaggtg gtgctctaac caactgagct acgtgccttc
     3421 aaagaatttt tgcattttat cgggtcgttt taatttttgc aagaggtatg tgcatttctt
     3481 tatgcaacat tgtggcaaga tttggggatg tcgtctgaaa aaccgtggga attcatttat
     3541 aatgccgtct tttaaaacat atttcgcccc ctgtcgccca actgacattc aatcttatgc
     3601 ttaaatttac cttacacaaa aaagacggat acgcccgacg cggcacgctg gagttgaacc
     3661 acggcaaaat tgaaacgccg gtatttatgc ctgtcggtac ttacggctcg gttaaggcga
     3721 tgaacccgca aaacctgcac gacatcaagg cgcaaattat tttgggcaac acttatcatt
     3781 tgtggctgcg tccgggcttg gaggtcgtcg aacagtttgg agggcttcac ggctttatcg
     3841 gctgggacaa accgattctg accgactcgg gcggttttca ggttttttct ttgtcggata
     3901 tgcgcaagct gacggaagaa ggctgtacgt tcaaaagccc gatcaacggc gacaagctgt
     3961 ttttatcgcc tgaaatttcc atgaaaatcc aaacggtttt gaactcggac atcgcgatgc
     4021 agctggacga atgcacgccg ggcgaagcaa cgcgcgaaca ggcgaaaaaa tcgctgcaaa
     4081 tgagcctgcg ctgggcggaa cggagcaaaa aagcctttga agatttgaaa aacccgaacg
     4141 cgctgttcgg catcgtgcaa ggcgcgatgt atgaggattt gcgcgaagaa tcgctgcgcg
     4201 gtttggaaca gtttgatttt cccggccttg ccgtcggcgg tttgtccgtc ggcgaaccca
     4261 agcccgaaat gtaccgtatg ctgcgcgccg tcggtccgat tctgccggaa cacaagccgc
     4321 attacctgat gggcgtaggc acgccggaag acctcgtgta cggcgtggca cacggcatcg
     4381 atatgttcga ctgcgtgatg cccacccgca atgcacgcaa cggctggctg tttacccgtt
     4441 tcggcgattt gaaaatcaaa aacgccaaac acaagctcga caagcgtccg atagacgaaa
     4501 gctgcacctg ctacgcctgc caaaacttca gccgcgccta cctgcaccat ctgcaccgca
     4561 ccggcgaaat cttgggcgca cagttgaaca ccatccacaa tctgcatttc taccaagtca
     4621 ttatggcgga aatgcgcgaa gccgtcgaac aaggcaaatt tgccgactgg caggcgcgat
     4681 tccacgaaaa ccgcgcacgc ggcacagact gattccctgc aatgccgtct gaagccgctt
     4741 cagacggcat tttgccgctt taccctaccc gcactatttt tcagaaaccg atcatgcttc
     4801 cattctttcc agaaccctcc ctttcctata cgcaacaaaa ccgtaccgcc gttttgctgt
     4861 tgaacctcgg cacgcccgac gcgccgaccg cgcaggcggt acgaccttat ctgaagtcct
     4921 ttctgaccga ccggcgcgtc gtcgaactgc ccaaatggct gtggtatccc attctgcatg
     4981 gtctggtact gacgctccgc cccaaaaaaa gcgcgcacgc ctatgaaaaa atctggttta
     5041 aggaaggttc gcccttagaa gtttacacag cacgccaagc tgccgcgctt gccaaacgta
     5101 tgcccgacct catcgtccgc cacgccatga cctacggcaa tccttcggtg gcagacgtat
     5161 tgtcggaact gaaggcgcaa ggcgcgggca gactgctggt catcccgatg tacccgcaat
     5221 atgccgcttc cagcagcgga gcggcagtag ataaggtgtg cgaacaatta ctgctgcaac
     5281 ggaaccaaat gagcgtccgc accgtttcgc gcttttatga cgataccggc tacattgatg
     5341 cgatgaaaaa ccacatcctc cgatattggg cggaacacgg gcgcgggaaa aaactgatgt
     5401 tgagttttca cggcgtaccg cagaagcacc acgacctcgg cgacccctat cccgacgagt
     5461 gccgccacac cgccaaactg cttgccgaag cactggaact gaccgaagac caatatgtcg
     5521 tatcgttcca aagccaattc ggcagggcaa aatgggttac accgagcacg caggatttgt
     5581 tcggcaaact gcccaaacag ggggtaaccg agctggacgt attttgcccg ggctttttgg
     5641 cagactgttt ggaaaccatg gaagaaatcg ccctgatggg gcgggaacag ttttatgaag
     5701 caggcggcaa aagctaccgc tacatcccct gcctcaacga caaccccgac tggatagatg
     5761 cactcgtcgc acttgccgaa gaaaaccttg gcagctggcg ttgaaactgt ttgacccatg
     5821 tctaaaatgc cgtctgaacg ccgtgttcag acggcatttt cccgctactt tagcgtatga
     5881 tggcatctat gctggacaac aacatcaagt gcaaccttat agtggattaa atttaaacca
     5941 gtacggcgtt gcctcgcctt gccgtactat ttgtactgtc tgcggcttcg tcgccttatc
     6001 ctgatttttg ttaatccact atactactga ttcggcaaaa aaatctttca cccgctacgc
     6061 aaaatcaaca acaaggtttc aaacatgaag gaaacaccaa tgaacacaac ccgactgccg
     6121 accgccctcg tcttgggctg cttctgcgcc gccgcttctg ccgccgacaa cagcatcatg
     6181 acaaaagggc aaaaagtgta cgaatccaac tgcgtcgcct gccacggcaa aaagggcgaa
     6241 ggccgcggaa ccatgtttcc gccgctctac cgctccgact tcatcatgaa aaaaccgcag
     6301 gtgctgctgc acagcatggt caaaggcatc aacggtacaa tcaaagtcaa cggcaaaacc
     6361 tacaacggat tcatgcccgc aaccgccatc agcgatgcgg acattgccgc cgtcgccact
     6421 tatatcatga acgcctttga caacggcggc ggaagcgtta ccgaaaaaga cgtaaaacag
     6481 gcaaaaagca aaaaaaacta aacagacaaa atgccgtctg aaaccggcaa tccggcttca
     6541 gacggcattc aaatcaaacc tttctaacgc agcactttca gccccataga cccgatattg
     6601 ccgccaccgt caacctcctt tgcggtcagc ataaagccgc cgatgtcaat acggccgccc
     6661 tcgaccggct cctgactatc ggaacgctta tcgaaaagct cggcaaggct caaaccctct
     6721 tcgcccgctt ccagcctcaa accgtaagta agtgccaaat cacccgaacg tgctgccggc
     6781 gaaacgacaa actcaccgaa gaaatcaaaa tgctcacgga cgctaatacc cgtctcggta
     6841 aagtatttcg ccattttata gtgaa
//
LOCUS       DAXHNE010000108         6619 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.108, whole genome shotgun sequence.
ACCESSION   DAXHNE010000108 DAXHNE010000000
VERSION     DAXHNE010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.108"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            362..2143
                     /locus_tag="ACFM4Z_001579"
     CDS             362..2143
                     /locus_tag="ACFM4Z_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HFC6021590.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHAVLFADYGFGLPLTGVESERGGNLALHWFADCADTDAASWLARHSDDLPAGIST
                     PQSSVSETDYLDRIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDAEGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQIGKVCVPEPFKVSRFGSVWQMTSTIQAQALPDTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLEAEPRGLYTGSIGYLNPSSGGLGFEGAFNV
                     VIRTLSLTPLSDGIYQGVYGVGSGIVIDSDPAAEYRECGWKARFLNELRPDFGIFETL
                     RAENGRCTLLDRHLCRLKTSAQALNLPLPDGCENQIKQYIADLPDGAFRVKALLASDG
                     ISLSRAVLNRLTDKQRVIISPTILPAQNYLRRFKTTCRTVFDQAWQTAETQGAFDSLF
                     FNSDGILLEGGRSNVFVKHRGQWLTPSLDLDILNGIMRQAVLDEPQKYLQTNQVIETH
                     ITQKTLQEAEEIRLSNALRGVFAAALA"
     gene            complement(2202..3719)
                     /locus_tag="ACFM4Z_001580"
     CDS             complement(2202..3719)
                     /locus_tag="ACFM4Z_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6021591.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPTEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNRNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGIAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(4415..6049)
                     /gene="groL"
                     /locus_tag="ACFM4Z_001581"
     CDS             complement(4415..6049)
                     /gene="groL"
                     /locus_tag="ACFM4Z_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFC6021592.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGTVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEDKPAMPDMGGMGGMGGMM"
     gene            complement(6142..6432)
                     /gene="groES"
                     /locus_tag="ACFM4Z_001582"
     CDS             complement(6142..6432)
                     /gene="groES"
                     /locus_tag="ACFM4Z_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFC6021593.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGSRRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
BASE COUNT         1496 a         2008 c         1574 g         1541 t
ORIGIN      
        1 caacgaagcc gaagacagta cagatagtac ggaaccgatt cacttggtgc ttcagcacct
       61 gagagaatcg ttctctttga gctaaagcga ggcaacgctg tactggtttt tgttaatcca
      121 ctatacttgg agctggtctt gcttttcgcc taattctacg ttttcaaacg gttgcagctg
      181 gtggtctgtc ataaaggtct ccttattgta tttcaggttg gaaatcggaa tttgttttca
      241 caattttaca ccttcgcccc ccactttctc tacataaaat tacattttgc cgatatttgc
      301 cgaatcgtct gaaaacatgt gtaaaaagag cgtataatca aaacatttgc cccgaattgc
      361 catgccttat ttcgccctgt ttgacgatgc cgtaagcggc cgcgcaaaac gctatcaaaa
      421 tcatgtggaa agccgttttt tccgtcccga agaactcgat gctttggacg gcgcgctgca
      481 atcgggctgg caaaaagggc tgcatgccgt gttgtttgca gactacggat tcggtttgcc
      541 gctgacgggg gttgagtccg agcgcggcgg caaccttgcc ctgcactggt ttgccgactg
      601 cgccgacacc gatgccgcaa gctggcttgc ccgacactca gacgaccttc ccgccggcat
      661 ttccacgccg caatcctccg tatccgaaac cgattacctc gaccgcatcc gccaaatcca
      721 cgaagccatc cggcgcggcg acacctatca aatcaactac accactcgcc tgcacctgca
      781 agcctacggc aatcccgtca gcctctaccg ccgcctgcgc cagcccgtcc cctatgccgt
      841 cttgtcccac ctgcccgatg cggaggggca atccgcgtgg acgctgtgtt tctcgcccga
      901 actcttcctc aacatcgctt cagacggcac catcagcacc gaaccgatga aaggcaccgc
      961 gccgattttg ggcgacggac aagacgaacg ccgcgccgcc gaattgcaag ccgacccgaa
     1021 aaaccgcgcc gaaaacgtga tgattgtcga tttgctgcgc aacgacctcg gcaaaatcgc
     1081 ccaaatcggc aaagtatgcg tacccgagcc gtttaaagta tcacgtttcg gcagcgtttg
     1141 gcagatgacc agcaccatcc aagcccaagc cctgccggac acctcgttcg ccgacatcct
     1201 ccgcgccgcc ttcccctgcg gcagcatcac cggcgcgccc aaaaaaatga gtatgcagat
     1261 tatcgaatct ctcgaagccg aaccgcgcgg actttatacg ggcagcatcg gctatttgaa
     1321 cccgagttcc ggcggcttgg ggtttgaagg cgcgttcaac gtcgtcatcc gcaccttatc
     1381 gctcacgccg ctttcagacg gcatttatca aggcgtgtac ggtgtcggtt ccggcatcgt
     1441 catcgacagc gaccccgccg ccgaatatcg ggaatgcggc tggaaagccc gtttcctcaa
     1501 cgaattgcgc cccgacttcg gcatttttga aaccctgcgc gcggaaaacg gacgctgcac
     1561 cctgctcgac cgccacctat gccgtctgaa aacctccgcc caagccctca acctgcccct
     1621 gcccgacggc tgcgaaaatc aaatcaaaca atacattgcc gacttgcccg atggcgcgtt
     1681 ccgcgtcaaa gccctgctcg cttcagacgg catcagcctg tcccgcgccg ttttaaaccg
     1741 tctgaccgac aaacaacgcg tcatcatttc gccgaccatc ctgcccgcac aaaactacct
     1801 gcgccgcttc aaaaccacct gccgcaccgt cttcgaccaa gcgtggcaaa ccgccgaaac
     1861 acaaggcgcg ttcgacagcc tgtttttcaa ttcagacggc atcctgctcg aaggcggcag
     1921 aagcaacgtg ttcgtcaaac atcgcggaca atggctcacg ccctccttag atttagacat
     1981 tttaaacggc ataatgcgcc aagccgtatt ggacgaaccg caaaaatatt tgcaaacaaa
     2041 tcaagtaatc gaaacacaca tcacacaaaa aacactgcaa gaagccgaag aaatccgcct
     2101 ctccaacgcc ttgcgcggcg tatttgccgc cgcccttgcc tgaacgcgca aaaatgccgt
     2161 ccgaacctgt ttccaaagtt cggacggcat tatcccacca ttcaaaaccg ccaatccgcc
     2221 gacacaaaca cctcgctgtt gcggcgtttc gcatacggca cattactttc cgtcctgccg
     2281 aaacgataat tcaacgccgg tacgatacct ttgtacgaca acttgtcgtg gctcaaagcc
     2341 agcgagacat tccattcgcg gttgcgttgc gcctctgtcg agaaagccgc aatgccctta
     2401 tagttgcggc gggcataaga cgcggaaacc cgactgttca aaccgcccaa ctgccgccac
     2461 tcctgcgccc agccggcata aacaccgttg cgccggtagg cggcattatt gaccgcgccg
     2521 cccaccgttt cgcgtttcgg cacaaaccgc acaaactgcc agccgccgaa cacagttgcc
     2581 gattcgccca aacgttttgc cgacgaaaca taaaacccgt cctgcctgcc gttattgtat
     2641 tccgccctat cctgttcgcg gtagcgttgg cggtaatgtt ccagcgcgac cgaaaattgc
     2701 catcccgggt ttgggcggta agtatgggac aactgcacgc cgactccgtg cgccagcata
     2761 tacggcggca ggcggcggtt gtttacccgt tttgttttcg catcaaagcc gtcgctgccc
     2821 gacaactgca cctgataaaa cggcaaaatc cccgccgtct gccgtgcatt tttatactgc
     2881 caacccaaat acgctctgcc gaacccgtcg tcataagctg attttttact gaaataatag
     2941 ctcgtgccgc cgatattgga acggaacaac aaataatgat tatctgccaa cgccgtcagt
     3001 ttttccgcct cgatttcata attcaagcct gccgcccgct ccgcccggct gacactgcat
     3061 atctgccggc ctccgttttg ccggcaatac tgcggcgcgg cattattggc atttctattg
     3121 accgccggac tgatgccgcc cgaaaaacgc cagcccgtca gcccctccgt ttttttccga
     3181 aaacgcccca cattttccaa aaccggcgcc ggcaaatcca attttgccgc ctccgcaaaa
     3241 tgcctttctg ccgacttcag ccggaaatcg tcaaactccg ccgccgccaa atccagcaaa
     3301 atccgctcgt ctgccgcatt ttccccgtgc agttcccgat accgcgccac cgcctccgtc
     3361 ggccttcccg ccaatttcgc cagcaaagcc cgcgccctgc cgtataaaac cgcgtcataa
     3421 tccggcagct tggcatacaa atccgccaac gaagcgatta aatccgcctg attgccgttg
     3481 agcgcgtcgc gcaaactatg ttccaacatt ttcggatgcg ccaacaaaaa atccccgtca
     3541 accacgcgcg gggcatcatt ttcaactttc caatctgatt ccgcccactt atccgacacc
     3601 gaccgctgca cctgcaacaa tgccttgtca tccaaaatcg cgggcgcatc cgccccatag
     3661 gcggcagaaa cacctgccgc acaccaaaca accaaaaagc cgtatctgaa atacaacata
     3721 ccctgtcatt tacctttctg gcaaacacgc cgccgaagca cgtcaaacca tccgaaaaac
     3781 aggcagaaac ccgtgaaaac cggctttgcc gcctgaaagc aggcaaacaa aaaccgccgc
     3841 ccgattttca aagggcggat ttcacattta tagtggatta acaaaaatca ggacaaggcg
     3901 acgaagccgc agacagtaca aatagtacgg aaccgattca ctcgtgctta agcaccttag
     3961 agaatcgttc tctttgagct gaggcgaggc aacgccgtac tggtttttgt taatccacta
     4021 taacagcaac cctgtcgccg tcattcccgc aaaagcggga atgacgaagc tatccgcaca
     4081 gaaacctgca ccacgtcatt cccacgaaag tgggaatcca gaacgtaaaa tctgaagaaa
     4141 ccgttttatc cgatacgttt ccgcaccgac agacctagat tcccgctttc gcgggaatga
     4201 cggcggaaag gttgctgttt ttccgataaa ttcctgccgc tcttcgtttt tgggatggcg
     4261 ggaaataaaa caaaagcgcg cgtatcaaaa aacaaaaatg caaagaacgg cgttggcaat
     4321 tttaaggttt gcgccttagc cgcacttatt cgcaaaaaaa accgcacggc gttgaccgtg
     4381 cggtttttta tctgaaagct tcagacggca ttgcttacat catgccgccc ataccaccca
     4441 tgccgcccat atcaggcata gccggtttgt cttcagggat ttcagcaatc atgcagtctg
     4501 tggtcagcat caggccggcg atagacgcgg cgtgttgcag cgcggaacgg gttactttgg
     4561 cggggtcgag tacgcccatt tcgatcatgt cgccgtattc gccgctgcca gcgttgtaac
     4621 catagttgcc tttgccttcc aacactttgt tcacaaccac gctgggttcg ccgcctgcgt
     4681 tggcaacgat ttggcgcagc ggagactcaa cggcgcgcaa gacgatttgt acgcctgcgt
     4741 cttggtcggc attgccggtg tgcaggtttt ccagagcggc acgggcgcgc aacagggcta
     4801 cgccgccgcc tgcaaccacg ccttcttcaa cggctgcgcg ggtagcgtgc agcgcgtctt
     4861 ccacgcggtc ttttttctct ttcatttcca cttcggtcgc ggcaccgact ttgattactg
     4921 ccacgccgcc tgccagtttg gcaacgcgct cttgcagttt ttctttgtcg taatcgctgg
     4981 ttgcggtttc gatttgttgg cggatttcgg caacacgcgc ttcgatttgg gctgcgtcgc
     5041 cgaagccgtc gatgatggtg gtgttttctt taccgatttc gatgcgtttg gcctgaccca
     5101 agtcgtccaa agtcgctttt tccaaagaca ggccgacttc ttcggaaatc actgtgccgc
     5161 cggtcaggat agcgatgtct tgcagcatcg ctttgcggcg gtcgccgaag cccggagctt
     5221 taacggcaac ggttttcaga atgccgcgga tgttgttcac gaccaaagtc gccaaggctt
     5281 cgccttctac gtcttcagcg ataatcaaca gcggacggct ggctttggcc acttgttcca
     5341 aaacaggcag caggtcgcgg atattgctga tttttttgtc gaacagcaat acaaacggat
     5401 tgtccaagcc ggcgatttgt ttttccgcat cgttgatgaa gtaaggagac aggtagccgc
     5461 ggtcgaattg cataccttca accacgtcca gctcgttttc caaagatttg ccgtcttcaa
     5521 cggtaatcac gccttctttg ccgacttttt ccatcgcttc ggcaataatc gcgccgactt
     5581 gttcgtcaga gttggcggaa atagagccga cttgggcgat ttctttagaa gtgtcgcaag
     5641 gtttggcgat gtttttcagc tcttcaacca aagcggcgac ggctttgtcg ataccgcgtt
     5701 tcaggtcggt cgggttcata ccggcggtaa cgtatttcat accttcggca acgatggatt
     5761 gcgccagtac ggtggcggta gtcgtaccgt cgcccgccac gtcgttggtt ttggacgcga
     5821 cttcttttac catttgcgcg cccatatttt caaacttgtc tttcagttcg atttctttag
     5881 cgacggttac gccgtctttg gtgatgtgcg ggccgccgaa agcgcggtca acgactacgt
     5941 tgcgaccttt aggacccaag gttacgcgca cggcgtttgc cagaatgttc acgccgttta
     6001 ccattttttg gcggacttca ttgccgaact gtacgtcttt tgctgccatt tcaattctcc
     6061 aaaaatcatt aaaactgtct gataaaaccg tttatgccgt ctgaaggcgg tttgccgttt
     6121 cagacggcat cgtgtccgta tttatttttc aacgatgccg aaaatatctt cttcgcgcat
     6181 taccaacagc tcttcgccgt cggctttgac ggtttggccg ctgtatttgc cgaagatgat
     6241 tttgtcgccg actttgacat ccagcggacg gcggctgccg tctttaccga ttttgcccgc
     6301 gcccacggcg atgacttcgc ccatatcggg tttttcggcg gccgcacccg gcaaaacgat
     6361 gcccgatgcg gttttttctt cagcttccaa gcgtttgacg acaacgcggt cgtgtaaagg
     6421 acggatggtc atatttatgc tccgataaaa tgatttgaaa acaatcatct gcccgaacgg
     6481 ttcggacaga ttgaagtgga aaccgggctg ccgcctgccg gcccgcccgt ataagtcggc
     6541 aaaattaggg tgtgtgcggg caaattcaag tgaggcggaa aaaatttatt tccgccgttt
     6601 tttatagtgg actaaattt
//
LOCUS       DAXHNE010000109         6413 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.109, whole genome shotgun sequence.
ACCESSION   DAXHNE010000109 DAXHNE010000000
VERSION     DAXHNE010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.109"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            89..298
                     /locus_tag="ACFM4Z_001583"
     CDS             89..298
                     /locus_tag="ACFM4Z_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HFC6021594.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            387..1601
                     /locus_tag="ACFM4Z_001584"
     CDS             387..1601
                     /locus_tag="ACFM4Z_001584"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFC6021595.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKSYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVYQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(1688..4267)
                     /gene="clpB"
                     /locus_tag="ACFM4Z_001585"
     CDS             complement(1688..4267)
                     /gene="clpB"
                     /locus_tag="ACFM4Z_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFC6021596.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEIDG
                     LEKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGIQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQDNIRNIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            4528..5538
                     /gene="trpS"
                     /locus_tag="ACFM4Z_001586"
     CDS             4528..5538
                     /gene="trpS"
                     /locus_tag="ACFM4Z_001586"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFC6021597.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFRELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETVEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            5544..5885
                     /locus_tag="ACFM4Z_001587"
     CDS             5544..5885
                     /locus_tag="ACFM4Z_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021598.1"
                     /translation="MPSEPLKGLPEQNVVRLTGKHPNDLEAVSGKCMETDDKGGPVGW
                     AENGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            6205..>6413
                     /locus_tag="ACFM4Z_001588"
     CDS             6205..>6413
                     /locus_tag="ACFM4Z_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021599.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASA"
BASE COUNT         1535 a         1678 c         1623 g         1577 t
ORIGIN      
        1 gatttgaatt tttcagaaaa tgaagcggac gtttgggcgg aggcaacttg tttgataaga
       61 tagcaatatt tcaaaacgga gaaagatcat gccttatgtc aatattaaag taaccggcgg
      121 caaggaagca ccgactgccg ctcaaaaagc ggaactgatc ggcggcgtaa ccgaattgct
      181 ggcacgcgtg ctgggcaaaa atcccgaaac aacggttgtc gtgattgatg aagtggatac
      241 cgataactgg ggaataggcg gcaaaagcgt cagcgaacgg cgcaaagagg gcaggtaaaa
      301 agcctgaaaa tctcggtttg atgctttaaa ttccgcgtga aaaagagtac attcccaccc
      361 attgcccaaa atttacggaa cacatcatgg ataaatttcc caagtctgca aagctcgatc
      421 acgtctgtta cgacatacgc gggccggttc acaaaaaagc acttcagttg gaagaggagg
      481 gcaataaaat ccttaaactc aatatcggca accctgcgcc gttcggcttt gaagcccctg
      541 atgaaatctt ggtcgatgtc atccgcaacc tgccgacttc gcaaggctat tgcgattcta
      601 aagggctgta ttccgcccgc aaagccattg ttcactacta tcagaccaaa ggtttgcgcg
      661 atattacggt tgatgatgtc tatatcggca acggcgtgtc cgagctgatt acgatgtcta
      721 tgcaggcgtt gctcaacgac ggcgacgaaa tcctgattcc cgcgcccgac taccctttgt
      781 ggacggcggc ggcaacgctt gcgggcggta cggtacgcca ttatctgtgc gacgaagaaa
      841 acggctggtt ccccaacctt gccgatatgg aagccaaaat cacgcccaaa accaaagcca
      901 tcgtcgtcat caatcccaat aatccgacag gcgcggtgta cagcagggaa atcctgttgg
      961 aaatcgccga actggcgcgc aagcacggtt tgattatttt cgccgacgag atttacgaca
     1021 aaatccttta tgacggcgcg gttcaccacc atattgccgc gcttgccccc gaccttttga
     1081 cggtaacgtt caacggtttg tccaaatcct accgcgtcgc cggcttccgc caaggctgga
     1141 tggtgctcaa cgggccgaaa catcacgcaa aaggttacat cgagggtttg gatatgctct
     1201 cgtccatgcg cctgtgtgcc aatacgccga tgcagcacgc gattcagacg gcattgggcg
     1261 gttatcagag cattaacgaa ttcattttgc ccggcgggcg gcttttggaa cagcgcaaca
     1321 gggcgtggga actggtcaac cagattcctg gcgtgtcctg cgtcaaaccg atgggtgcga
     1381 tgtatatgtt cccgaaaatc gataccgaaa tgtaccgcat ccgcgatgac atgaaattcg
     1441 tttacgattt gctggtgcgc gaaaaagtct tgctggtgca gggaacgggg tttaattgga
     1501 tcaagcccga ccatttccgc attgttacgc tgccttacgt ctatcagatt gaagaggcga
     1561 tgggcaggct ggcaagattc ctgcaaacct accgccaata aggggacggt ttgtctgccg
     1621 aggataaaaa atgccgtctg aaacggagat tcccgtttca gacggcattt tcaacagcag
     1681 gaacgaatca ggcaaatttc agtctgtcgc cgtcggcttc caccctgatt tcgctttcgg
     1741 gcgcatagtt tccggcaagc agggcttttg ccagcgggtt ttcgatttcc gactggatgg
     1801 cgcgtttgag cggacgtgcg ccgtaaatcg ggtcgaaacc ggctttggcg atgatgtcca
     1861 gagcggcatc ggaaacagtc aggcggaggt tttgtttttc caaacgtttt tccaagcctt
     1921 tgagctggat tttcgcaatg ttgcggatat tatcctgatc cagtccgtgg aacacgacca
     1981 cttcgtcgat gcggttgatc atttcaggac ggaaatgttc tttcacatcc tccatcacaa
     2041 cttcttttac cgcttcgtaa tcctgaatgc ccatttgttg gatgtgctgg ctgccgatgt
     2101 tggaagtcat cacaatcacg gtatttttaa aatctaccgt cctgccttgc ccgtcggtca
     2161 aacgaccgtc atccaatact tgcagcagga tgttgaacac gtcgggatgg gctttttcca
     2221 cttcgtccaa aagaatcacg ctgtacggtt tgcggcggac ttgctcagtc aggtagccgc
     2281 cttcttcgta gccgacatag cccggaggcg cgccgattaa gcgggcaacg gcgtgttttt
     2341 ccatatattc ggacatatcg atacgaatca gatgatcttc gctgtcgaac agaaagcctg
     2401 ccagggcttt acacaactcg gttttaccca cgccggtcgg gcccaagaac aggaagctgc
     2461 cgtaaggctt gttcggatcg gcaagaccgg agcggctgcg gcggatagcg tcggacacgg
     2521 cacgcacggc ttcgtcctgt ccgaccacgc ggcggtgcaa tacttcttcc attttcagca
     2581 gtttgtcgcg ttcgccttcc atcattttgg atacgggaat gccggtcata cgggaaacca
     2641 cctctgcgat ttcctccgcg ccgacattat tacgcaagag tttgtttgcc ggttttgtgc
     2701 tgtccgtatc tgcccgttcg gcggctgcac gctgtttttc caaatgctcc aaatcttcat
     2761 acatcaattt tgaagccagt gccaaatcgc cttgccgttt tgcctgttcg attttaattt
     2821 tgacttcgtc aatttgtttc ttaatattag cagcaccgtc tgaaattgct ttttcggctt
     2881 tccagatttc gtccaaatcg gcatattctt tttccaaacc gtcgatttcc tcatctatca
     2941 gttccaaacg ttttttgctg gcatcgtctt tttctttttc aacgtgcgcc ttttccatcc
     3001 gaagctgaat tagacggcgg tcgattttgt ccattgcttc cggcttggtt tctttttcca
     3061 tcttgacacg gctggcggct tcgtcaatca aatcaatcgc tttatcaggc aggaagcggt
     3121 cggtaatgta gcggtcgctc aactccgctg cggcaacgat agcagggtcg gtaatatcga
     3181 taccgtggtg gatttcataa cgctcctgta aaccgcgcaa gatagcgatg gtgtcttcta
     3241 cacttggctc gccaaccaat actttttgga agcggcgttc gagtgccgcg tctttttcga
     3301 tgtattggcg gtattcgtcc aaggtggtcg cgccgataca gtgcaattcg ccacgtgcca
     3361 aagccggttt cagcatattg cccgcatcca tcgcaccgtc ggttttgcct gcgccgacca
     3421 aagtatggat ttcgtcgatg aaaatcagag tgttgccgtc gtctttcgcc aagtcgttca
     3481 acacgccttt caagcgttct tcaaattcgc cgcggtattt cgcgccggca atcaatgccg
     3541 ccaagtccaa aaccagtagg cgtttgttgc gcagggattc aggtacttcg ccattgacga
     3601 tgcgttgtgc caaaccttcg acgatggcgg ttttacccac gcccggttcg ccaatcagca
     3661 cagggttgtt tttggtacgg cgttgcaata cctgaatcgc acggcggatt tcgtcgtcac
     3721 ggccgataac ggggtcgagc ttaccgtctc gggcgcgttg ggtcagatcg agcgtatatt
     3781 ttttcaaagc atcgcgttgg tcttcggcat tggcatcgtt cacgttttgt cctcctcgta
     3841 ccgcgtcaat cgcggcattg atgttttgtt cggtcgcgcc ggcttctttc aaaattttgc
     3901 cggccgcatc gttctgctgc accaaggcaa gcaggaaaag ttcactggcg atataggcat
     3961 cgccgcgttt ggttgcggct ttgtccatca ggttcaacac cgcctgcaat tcgcggctgg
     4021 gcagaatgtc gccgccctgg ccggacactt tcggcaggct gttcaaatgc tgctgcaaac
     4081 gctgtttcac ctgcggcacg ttcacgcccg catgagccaa gagcgcggca gcaccgctgt
     4141 tttggtcgtc aagcagggct ttcagcacaa agcccgcttc cagatagctg ccgtccgcag
     4201 ccaacgccaa actctgagct tctgcaaggg cctgttggaa tttggcggtt aatttgtcgt
     4261 atcgcatttt tgtttccttt tcaaaatgtc cgctgtcgaa gcctatatgt gcataattgt
     4321 ggataactca agttctgttt tctgtttttc tatatttaat tcgatatatc attgaattta
     4381 aagtatataa aaatgtataa taatgtgtat aactatatct tcttaatatg gaaaagtctg
     4441 ttgtcggctg gatgtaggtg gcaaatcggg tataatcggc gcatcttttt ccctttcaga
     4501 cggcattgat gccgcaagga catttttatg agcaaaaaac gagttctgac cggcgtaacc
     4561 accaccggca tcccgcatct gggcaactac gtcggtgcta tccgccccgc cgtccgcgcg
     4621 gcgcaaaacc ctgataccga atccttcctc ttcctcgccg attaccacgg tatcatcaaa
     4681 tgccacgagc cggagatgat tcaccaatcc acccaagccg ttgccgccac ttggcttgcc
     4741 tgcggactcg accccgagcg caccaccttc taccgccaaa gcgacattcc cgaagtgatg
     4801 gaattgaact ggattctgac ctgcatcacc gccaagggtt tgatgaaccg cgcccacgcc
     4861 tacaaagccg ccgtgcaggc aaatgcggaa aacgggcagg aagaccccga tttcggcgtg
     4921 gaaatgggtt tgttcagtta tccgattctg atgactgccg atattctgat gttcaacgcc
     4981 aacgaagtcc ccgtcgggcg cgaccaaatc caacacgtcg aaatggcgcg cgacatcgcc
     5041 ggccgcttca atcaccgctt ccgagaactc ttcaccctgc ccgaagtgaa aatcgacgaa
     5101 aacgtcgaac tcttggtcgg tttggacgga cgcaaaatgt ccaaatccta cggcaacacc
     5161 attccgcttt gggaaaacga caaaaaaacc caaaaatcgg tcaacaaaat catcaccaat
     5221 atgaaagagc cgggcgagcc gaaacagccc gacgaaagcc cattgtttga aatctacaaa
     5281 gccttctcca cgccgtctga aacggtggaa tttacgaaaa tgcttgccga cggcctggct
     5341 tggggcgagg caaaaaaact tttggcggcg aaaatcaacg ccgaactcgc cgaaccgcgc
     5401 gaacgctaca acgagctgac cgccgaccct tcgcaaatcg aagagatttt acaggcaggt
     5461 gcgcaaaaag cgcgtaaaga agcacgcgaa ttgttggaca aagtacgcga tgcggtcggc
     5521 atccgcccgt tgaaataaac ccgatgccgt ccgaacctct taaaggcttg ccggaacaaa
     5581 acgtcgtccg gctgaccggc aagcatccca acgacttgga agccgtcagc ggcaaatgca
     5641 tggaaaccga cgataagggc ggtccggtag gctgggcgga aaacggcgtg tgccatacct
     5701 tgtttgccaa actggtgggc aatatcgccg aagacggcgg caaactgacg gattacctga
     5761 tttcgcattc cgccctgcaa ccctatcagg caggcaaaag cggctatgcc gccgtgcaga
     5821 acggacgcta tgtgctggaa atcgacagcg agggggcgtt ttatttccgc cgccgccatt
     5881 attgaggtat tcggacatcc cggaatatat ttgggttttt caaaccttgc gaaaaaaagt
     5941 ccgcaccgca gggaaactca aaaggagggg gatatgtgtt acaattttcc gaactgtttt
     6001 cataaaatag ttttcggacg tgtttcaata tggcattgat gccgccttat tgttcgagaa
     6061 aaaaaccttt atatttaaat ataatgggtt ttaactaaac gggaaaccgt tttctctccg
     6121 gccgatgggc aaaatcagcc gattgatgga acacggttcg atttttaagc aaaaccttaa
     6181 tcaacaactc aagaaggata agacatgaaa aaattattga ttgccgcaat gatggcggct
     6241 gccttggcag cttgttcgca agaagccaaa caggaggtta aggaagcggt tcaagccgtt
     6301 gagtccgatg ttaaagacac tgcggcttct gccgccgagt ctgccgcttc tgccgtcgaa
     6361 gaagcgaaag accaagtcaa agatgctgcg gctgatgcaa aggcaagtgc cga
//
LOCUS       DAXHNE010000110         6324 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.110, whole genome shotgun sequence.
ACCESSION   DAXHNE010000110 DAXHNE010000000
VERSION     DAXHNE010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6324
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.110"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            61..444
                     /locus_tag="ACFM4Z_001589"
     CDS             61..444
                     /locus_tag="ACFM4Z_001589"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFC6021600.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            737..1210
                     /locus_tag="ACFM4Z_001590"
     CDS             737..1210
                     /locus_tag="ACFM4Z_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HFC6021601.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVEGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMPAMNHGHHHGEAHQH"
     gene            1336..2439
                     /gene="mnmA"
                     /locus_tag="ACFM4Z_001591"
     CDS             1336..2439
                     /gene="mnmA"
                     /locus_tag="ACFM4Z_001591"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFC6021602.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDDETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            2510..4180
                     /locus_tag="ACFM4Z_001592"
     CDS             2510..4180
                     /locus_tag="ACFM4Z_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFC6021603.1"
                     /translation="MEKIWLDSYEKGVSAEIDITQYNSVSDVFRQSVEKFADQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPVALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFVIRKIKKMVPEYRIRETVSFQTVLKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSELRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            4292..5926
                     /locus_tag="ACFM4Z_001593"
     CDS             4292..5926
                     /locus_tag="ACFM4Z_001593"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFC6021604.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSENIEK
                     NKGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESTDLGGTMRLGAQEVELK
                     AGSLAAKIYGSGHIRERHRHRYEVNNNYVPTLEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
     gene            6154..>6324
                     /locus_tag="ACFM4Z_001594"
     CDS             6154..>6324
                     /locus_tag="ACFM4Z_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6021605.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDRVIDWQLIEQYLNRQKTR
                     YLRDHRGRPAYPL"
BASE COUNT         1663 a         1708 c         1586 g         1367 t
ORIGIN      
        1 ggcaacgccg tacaggtttt tgttaatcca ctatattgag aacattttga aaggataccg
       61 atggaacctt cctcctacgc ggcagaaaaa aaaggcaaag gcggcatcag gcgcgtcatt
      121 aacgcattcg gctattcgat agacggcatc gccgccgcct accgttacga agcggcattc
      181 cgtcaggttt tgtggctgaa cgtactgctg gtgtgcgcgg catttttttg ggtttccgaa
      241 accgccgtcc gcctgccgtt gattatcgcg tcttttgtgt cggtcattgt cgaactgttc
      301 aacaccgccg tcgaagctgc cgtcgatcat acttcgaccg aaaaacacga gctggccaaa
      361 cgcgccaaag acgcaggttc tgccgcacaa ttgttcgcga tgctgatgtt ggcggcggtt
      421 tggctgtctg ccctgttcgg gtaaaacgct tgcagcagga tttgtaatcc tttaggatta
      481 gtatgtggta tttattattc acttaatcta catcaaattt ccaggcagta tttgtatgta
      541 tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacgg
      601 aaccgatcca cttggtgctt cagcacctta gagaatcgtt ctctttgagc taaggcgagg
      661 caacgctgta ctggtttttg ttaatccgct ataagattaa gcacattccc cgtctgatat
      721 tataaggagc agaaagatga aaaaattatt ggcggccgtg atgatggcag gtttggcagg
      781 cgcggtttcc gccgccggag tccacgttga ggacggctgg gcgcgcacca ccgtcgaagg
      841 tatgaaaata ggcggcgcgt tcatgaaaat ccacaacgac gaagccaaac aagacttttt
      901 gctcggcgga agcagccccg ttgccgaccg cgtcgaagtg catacccaca tcaacgacaa
      961 cggcgtgatg cggatgcgcg aagtcgaagg cggcgtgcct ttggaagcga aatccgttac
     1021 cgaactcaaa cccggcagct atcatgtgat gtttatgggt ttgaaaaaac aattaaaaga
     1081 gggcgataaa attcccgtta ccctgaaatt taaaaacgcc aaagcgcaaa ccgtccaact
     1141 ggaagtcaaa atcgcgccga tgccggcaat gaaccacggt catcaccacg gcgaagcgca
     1201 tcagcactaa tctgctgaaa atgtttgaaa tgccgtctga aaaagcccgg gctttcagac
     1261 ggcattttta tgcccgcctt taaaatgtgt taaaatccgc ctttaaaaac cgccgtttcc
     1321 aaccatattc tacgcatgaa tacgacagca aacccttcca atatcatcgt cgggctctcc
     1381 ggcggtgtcg attcttccgt aaccgccgcc ctgctcaagc agcagggtta tcaagtgcgc
     1441 ggtgtgttca tgcagaactg ggaagacgac gacaacgacg aatattgcag catcaaacag
     1501 gattcgttcg atgccatcgc cgttgccgat attatcggca tcgatatcga catcgttaat
     1561 ttcgccgcgc aatataaaga caaagtcttt gcttattttc ttcaggaata cagtgcgggg
     1621 cgcacgccga atccggatgt gttgtgcaac gccgaaatca aattcaaatg ctttttggac
     1681 tacgccgtag ggcagggcgc ggataccatc gccaccggcc actatgcgcg caaagaagtt
     1741 cgcaacggcg tgcattacct gctcaaaggt ttggatcgaa acaaagacca aagctatttc
     1801 ctctaccgcc tcaagccttt ccaactcgag cgcgcgattt ttccgttggg cggtttggaa
     1861 aaacccgaag tgcgccgcct tgccgccgaa tttaatttgc cgactgccgc taaaaaagac
     1921 agcacgggca tctgtttcat cggcgaacgc ccgtttcgcg agtttctgca gaaatacctg
     1981 ccgaccgaca atggtaaaat ggttacgccc gaagggaaaa ccgtcggcga acacgtcggg
     2041 ctgatgttct acacgctcgg acagcgcaaa ggtttgggca ttggcggcgc gggagaaccg
     2101 tggtttgttg cggctaaaga tttgacgaaa aacgaactga ttgtcgtgca aggacacgac
     2161 catccgctgc tttatacccg cagccttgtg atgaacgatt tgagtttcac gctgcccgaa
     2221 cgcccgaagg caggacgcta tacctgcaaa acgcgttacc gtatggcgga cgcgccttgt
     2281 gagttgcgct atttggatga tgaaaccgcc gagctggtgt ttgacgaacc gcaatgggcg
     2341 gttacgccgg gtcagtccgc cgtgctgtac gacggcgata tctgtttggg cggcggtatc
     2401 atccaaacga ccgacaaacc cgtcatcatc acacgataaa ggtaatgccg tctgaaacgg
     2461 ttttcagacg gcattgttcc gctcaattcc actttaaaga ccgataccta tggaaaaaat
     2521 ctggttagac agctacgaga agggcgtcag tgccgaaatc gacatcacgc aatacaattc
     2581 cgtcagcgac gtattccgcc aaagcgtgga aaaatttgcc gaccaacctg cttttcaaaa
     2641 catgggtaag acgctgactt atgccgaaac cggcaaactg gcaacagatt ttgcttctta
     2701 tctgcaaaac gtccttaaat tgcctcgagg agagcgcgtg gcaataatgt tgcccaatgt
     2761 actgcaatat ccggttgccc ttttcggcat tttgcaggca ggtttggtag cggtgaacac
     2821 caatccgctc tataccccgc gcgagctgga gcatcagttg aaagacagcg gcgcgaccgc
     2881 catcatcgtt ttggaaaatt tcgccaacac gctggagctg gtgctgccgc gcacgcagat
     2941 caaacacgtc atcgtcgcct ccgtcggcga aatgttcggg ctgcttaaag gttcgctgat
     3001 caatttcgtc atccggaaaa tcaagaaaat ggtgcccgaa taccgtattc gggaaaccgt
     3061 ttcctttcag acggtattga aagagggggc gaagcacgtt ttccaacctg tcgcattaaa
     3121 ccgtgaagat accgcgctgt tgcaatacac gggcggcacg acaggtgttg ccaaaggcgc
     3181 agtgctgagt cacggcaaca tctgcgccaa tatgcttcag gcaaaagaat ggattaaaaa
     3241 ccagttgcgc gaaggcaaag aaaccgttat cgccgccctg ccgctgtatc atatttttgc
     3301 cttaaccgtg aatctgatga tttttgccaa tgccggctcg aaaatcatcc tgattaccaa
     3361 cccgcgcgat atgaaaggct ttatcggaga actgaaaaaa cagcgggtta atgtatttat
     3421 cggcgtgaac acgctgttta acgcgatggt taaccgtcct gattttgccg aagtcgattt
     3481 ttcagaattg cggctgactt tgggcggcgg tatggcgacc caaaaagccg ttgccgaaaa
     3541 atggaaaaaa atcacaggca cgcccatcgt cgaagcctac ggtctgaccg aagccagccc
     3601 cggcgtgtgc tgcaacccct taaacatcga atcatacagc ggcagcatcg gtttgcccgt
     3661 ctcgtccacc gaagtcgaac tgcgcgacgc aaacggcaaa gaagtccccg tcgggcagcc
     3721 gggcgaattg tgggtaaaag gccctcaagt gatgcaaggc tactggaacc gccccgaaga
     3781 aaccgccaaa gccatagacg cgcgcggctt cttggaaacc ggcgatattg ccgtcatgga
     3841 cgaaaaaggc tggttgaagc tggtcgatcg taagaaagac ctcgtcgttg tttccggatt
     3901 caatgtttat ccgaacgaaa tcgaggaagt catcgcgcat cacggcaaag tgatggaagt
     3961 cgcctgtatc ggcgttcccg acgaaaaaac cggcgaggca ctcaaagtat tcgtcgtcaa
     4021 aaaagacccg tctttgacca aagaagaact tatcgccttt tgccgcaccg aattaaccgc
     4081 atataaagta ccgaaaaata tcgaattccg agacgagttg cccaagtcca acgtcggcaa
     4141 aatcctgcgc cgcgagttgc ggcaaagtac cgggaaataa agaaaagatg ccgtctgaaa
     4201 acagccgtcc accgttcaga cggcatccgt ccgtttgcca gaaccgcgcg cttcacgtta
     4261 aaatcacgca ttccaatacg ggtatttcat tatgaccaaa ttcattttcg taaccggcgg
     4321 cgttgtctcc tcactgggta aaggtatcgc cgccgcttct attgccgcca tcctcgaatc
     4381 gcgcggcttg aacgttacca tgctcaagct cgatccttat atcaacgtcg atcccggcac
     4441 gatgagcccg ttccaacacg gcgaagtgtt cgtaaccgac gacggcgcgg aaaccgacct
     4501 cgacttggga cactacgaac gctttatcga ttccacgatg acccgccgca acagcttcag
     4561 cacggggcag gtgtacgaaa acgtcatcgc caaagaacga cggggcgact acctcggcgg
     4621 cacggttcaa gtcatcccgc acattaccga cgaaatcaaa cgccgcatcc acgaaggcgc
     4681 ggcaggttac gatgtggcga ttgtcgaaat cggcggcacg gtcggcgaca tcgaatcgct
     4741 gccgtttttg gaagccatcc gccagatgcg aagccagttg gggcgcaaca acaccttgtt
     4801 tgcccacttg agctacgtcc cctacatcgc cgccgcaggc gaaatcaaaa ccaagccgac
     4861 ccagcacacc gttaaagaaa tgttgagcat cggtttgcaa cccgacatcc tgatttgccg
     4921 tatggacagg acaatgcctg cggacgaacg ccgcaaaatc gccttgttct gcaacgtgga
     4981 agaacgcgcg attgtcggca gctacgatgt ggacagcatc tacgaatgcc ccgaaatgct
     5041 gcacgaccaa ggcatcgaca acatcattac cgagcagttg cagcttaatg tgcagcaggc
     5101 ggatttgacc gcgtggaaaa aaatcgtcca cgccatccaa aacccgaaac acaccgtcaa
     5161 aatcgccatg gtcggcaaat acgtcgattt gaccgagtcc tacaaatcgc tgatcgaagc
     5221 cttgaaacac gcaggcattc acaccgaaac cgacgtgcag attacctttg tcgacagcga
     5281 aaacatcgag aaaaacaagg gcgacgtttc catgcttaaa gacatggacg ccatcctcgt
     5341 tcccggcggt ttcggttcgc gcggtgtgga aggcaaaatc gccgccgtgc gctacgcccg
     5401 cgaaaacaac gtgccatact tgggcatctg cctcggtatg cagattgcgc tgatcgaata
     5461 cgcccgcgac gtggcaggct tgaaaggcgc gaattccacc gagttcgacc tcaaatgcgc
     5521 cgcgccagtc gttgccctga ttgacgaatg gcaaactgcc gacggcagcg tcgaaacccg
     5581 cgacgaatcc accgatttgg gcggcacgat gcgtttgggc gcgcaagaag tcgaattgaa
     5641 agcaggcagc ctcgccgcca aaatctacgg cagcggacat atccgcgaac gccaccgcca
     5701 ccgctacgaa gtcaacaaca actacgttcc tacactggaa caggcaggtt tggtcatcgg
     5761 cggtgtatct gccggacgcg aacgcttggt cgaaaccatc gaactgccga accatccttg
     5821 gttcttcgcc tgccagttcc accccgagtt tacctccaat ccgcgcaaag ggcatccttt
     5881 gttcaccgcg tttgtcaaag ctgcgttgaa caataaaaaa gcctgataaa gcgttacttg
     5941 atgatcaaaa tgccgtctga aagcctgaat gaagactttc agacggcatt ttcgcaaatc
     6001 ggggattata aaagccatca atttaatttt tatggtttta tctaatattt accaactgaa
     6061 tattgttaag cggggcgtga aactcaagtt gagacctttg caaaattccc caaaatcccc
     6121 taaattccca ccaagacatt taggagattt ctcatgagca ccttcttcca gcaaaccgcc
     6181 caagccatga ttgccaaaca catcgaccgc ttcccgctat tgaagttgga ccgggtgatt
     6241 gattggcagc tgatcgaaca atacctgaac cgtcaaaaaa cccgttacct tagagaccac
     6301 cgcggccgtc ctgcctatcc cctg
//
LOCUS       DAXHNE010000111         6280 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.111, whole genome shotgun sequence.
ACCESSION   DAXHNE010000111 DAXHNE010000000
VERSION     DAXHNE010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6280
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.111"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..823)
                     /locus_tag="ACFM4Z_001595"
     CDS             complement(<1..823)
                     /locus_tag="ACFM4Z_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021606.1"
                     /translation="MEIFEVNVYTKYNPKDPKQNIIGTVKASYVSDGAGGYLKDKNGN
                     PYIAPVGYNPHEAWAYGAENPLNLLNYGTGKEKDLQRSFHGKSYGGFVPAFQPIASFD
                     VGVACAGAIGVSKDICIAGGGAVNLRNSLSNSNINTEGKYYNNPPNVPNIENGYDWGK
                     NLGTFNDSLENSLKETLNNIRNKFKEIQNSFHNLTDRNISFDSESIGSLLKEKFEDWS
                     HRVYDWLPDDWNLRKELDRNRSGKYHVYDPLALDLDGDGIETVAAKGFSGSLFDHN"
     gene            complement(1522..2715)
                     /locus_tag="ACFM4Z_001596"
                     /pseudo
     CDS             complement(1522..2715)
                     /locus_tag="ACFM4Z_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
     gene            3991..6123
                     /locus_tag="ACFM4Z_001597"
     CDS             3991..6123
                     /locus_tag="ACFM4Z_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0030256 - type I protein secretion system
                     complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFC6021607.1"
                     /translation="MPTVPTPLPALSALIILAHYHGIAANPADIQHEFCASAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPVKRLAMATLPALVWCDDGNHFILAKTDGGGEHAQYLIQ
                     DLTTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
BASE COUNT         1747 a         1300 c         1334 g         1899 t
ORIGIN      
        1 cgttatggtc gaagaggctg cctgaaaagc ctttggcggc gactgtttct atgccgtcgc
       61 cgtctagatc tagggcgagg gggtcgtaaa cgtggtattt gcctgaacga tttctgtcta
      121 attctttccg tagattccaa tcatcaggta gccagtcata taccctatga gaccagtctt
      181 caaatttctc cttcaggaga ctgccaatgc tttcagaatc aaaagaaata tttctatctg
      241 ttaaattatg aaatgaattc tgaatctcct taaatttatt cctgatatta tttaatgttt
      301 cttttaatga attttctaaa gaatcattaa atgtaccaag gttttttccc caatcatatc
      361 cattttctat atttggaaca ttaggcgggt tgttatagta tttcccctca gtattgatat
      421 tactattaga taacgaattc cttaaattta ccgctccacc tccagcaatg caaatatcct
      481 tagaaacacc aattgcacca gcacaagcta ctccaacatc aaaagatgca atgggctgaa
      541 acgctggcac aaatccccca tacgattttc catggaatga acgctgcaaa tctttttctt
      601 tacctgtacc atagtttagt agattaagtg gattctccgc tccataagcc catgcttcat
      661 gagggttgta tccgacggga gcaatataag gattaccatt cttatctttt agataaccac
      721 cagctccatc tgaaacatac gaagctttaa ctgtaccaat aatattttgc ttaggatctt
      781 ttggattgta ttttgtatat acatttacct caaaaatttc cataactgag cctttacttg
      841 ttgataaaag ataaaataaa attagaaact aaaagtgaga aaaagttaat aataataggg
      901 atgtataaat gtaaaggttc tgtttcatag ctaaggttat ctgaatatat ggaaaaaaag
      961 taaaagtcca taaaagtaaa atatatagat aatgataatg ataatagaat tatcactttc
     1021 ctagagtaag gataatattt ttttacgata aaaaaatata aaataggata aataattgcc
     1081 atatagatag attttgtagc aaaattaaaa ataaataata aaaataatgt aataaagtat
     1141 attttttggc tatgaaataa aattgtacat aattgaacga gcagatcaaa aaatgaacta
     1201 catataacaa taaataataa cgtatttacc atactaaatt taataggtct cattatcata
     1261 tttaataacc acttcatagt aaatgcagag tgggtggaaa cactcacttt atggtttgct
     1321 acgctctgct caattagcaa cccgataacc caatatagat aatagggcaa ttaatccaat
     1381 ctaatttgtt aaaatacgtt aattttttgt aaaataaagt attggattat gtcgggtgca
     1441 aatgatgcct tttagattgc ctaaaaaaca aacaaacggt gtgtgtatag caagtactgc
     1501 actttgcggc ttactttctt taccgagatt tttatttaca tctttatagg cgagatccaa
     1561 agattgaata cccaattctt caagggtgcg gagttcgttg gtttgggaaa tgccgtcctg
     1621 attgagatcc tgccatacac gcagggattg gaatgcggcg tctgccgcgt tgatgatgtt
     1681 gtcgccgttt gaatccaatt cggccaaagc cgcgtagccg tgtttggcaa aagaaccgtc
     1741 tgccagtttg gtattgtcgc cgaagagttc cgcaccgttg tcgatgatgc cgttgccgtt
     1801 caaatcgcgc acaagcagac cgtcatcggc agaaacccaa ccggtggcgg tgcggatgcc
     1861 ttggttgcgg tggtcgaaca atgcacctga aaagcctttg gtggcaacgg tttctatacc
     1921 gtcgccgtct agatctaggg cgagggggtc gtaaacgtgg tatttgtcgc tgcggttaag
     1981 atttaagaaa tcctcaaacc atctccaagc tttttcagct cctaatgatc cccaccaagc
     2041 tcctgcgcca cctccaagta tagctccaac tactagccaa cctgcgccac cagtaagtaa
     2101 accaaaagcc ccaacaccca aagccgcacc ccattggacg cccattagcc cacccaaaaa
     2161 agaagctgat gagagaccaa catcactcca atttccttct ccatccaagg ctttccacca
     2221 agcacttccc atactcaaag ttcccatggc aacagttgcc ccataagtaa acttacccaa
     2281 attcgctgcc ataacttttg attttgaagc attctctaac gctgttgccg cacgtcttgc
     2341 atattgctgc ttgttatagt tgctagcatt caatgctttt tgcaaatcac tttgtacttt
     2401 tcttgcaaca aatgcctcct ttgaattacc acgaaattta ctagcagctc ttccaattcc
     2461 ttcttttaag cttttttgtg cctcttctgc tgtgcgttgg aattctcttg ccttagcaga
     2521 cagacgctca ccctccctta aaaattcttt ttgatctatc ttgtgcgaac gggtcaaata
     2581 ttcttcgtac attgagttaa tcgtaccaat cgccctaacc acattttcat cagaaaatat
     2641 ggaaataata gcatccctat acgcacctag tgtaatattg tttctattat tagttatagc
     2701 attattcgaa tacataatag cacctccaaa tttagaactg aatacgttta tattctctgt
     2761 gtaataatat caaataatta attaatggat aagtaagtcc gaaaaaataa caactaaaat
     2821 aagcaatata ctgattctct cctttattaa ttagatatat tgaaaaacaa ataaaaaata
     2881 aacagaaaac attcatgtga aaaagccacc tcatattctt atttataagt ttaaaataac
     2941 taggcattgt atatttaaaa atcggtacat ccaacggaaa caaaccacat aaaaattttt
     3001 ctttattctt tattaaagac atagttttat ttttcaaaat tagtccagat aatatactaa
     3061 caattactat taaaacaata agcacagcgt aattttttat aaaattcatt aaaataataa
     3121 tgaaaatact aaatagatta catactataa attgaaatat aaaatattca gtatttaatc
     3181 cattttcaac cgcgagtttt ttaaaatatc cttgctcgaa aaaattaaaa aaatccatta
     3241 atactttcct ctcaagaaaa ctaattttag tttagttaag ataaaatgca atattacctt
     3301 ttctccataa aagtatggag cttatataca taaattatgt gcgaggtagc ctgaaaaata
     3361 tccaatacaa gatagtagga gatgtttccc ttatcactat tggccaatac ccaattggca
     3421 ggcactctat ttccaaaatt ttctcaagaa tagttaaata ctattaatta gtttaaaaac
     3481 aataacatac gtttgtattt atccgttttg gtctgcctga aacagactct atgtccatac
     3541 acggacatgt agacagtttc cttgttattt tggttgctcg tcattagtga tgatacttta
     3601 ctcctctctt aaaaaatcgt caagaactgt tatcaatctt taaagtcttt tttaaacaaa
     3661 tatatatacg ttcaggctgt ctgaaacaca aaaaaatgat gttacccgac cgtgacagag
     3721 tcggggcagc cggtacctta accctgcacc ttaaccctgt gccttaacgg tatataggag
     3781 gcacgatagg tgcgaggcat ccgcatctat cttgattcct aagccgtttt accttcctga
     3841 aacgagaact tttccatact ttccctttac aattcctgta tagatcctta caatgtgttc
     3901 tgctgtttta tttatatatt tctttctact tagatatatt ctttttccag acagccgtta
     3961 tccacatcca atctaacata tattttattt atgcctaccg tacccacacc tctccccgcc
     4021 ctttccgccc ttattattct tgcgcattac cacggcattg ccgccaatcc tgccgatata
     4081 cagcatgaat tttgtgcttc cgcacaaagt gatttaaatg aaacgcaatg gcttttagct
     4141 gccaaatctt tgggattgaa ggcaaaggta gtccgccagc ctgtcaaacg tttggctatg
     4201 gcgaccttac ctgcattggt atggtgtgat gacggcaacc attttatttt ggccaaaaca
     4261 gacggtgggg gtgagcatgc ccaatatcta atacaggatt taactacgaa taagtctgcg
     4321 gtattgtctt ttgccgaatt ttctaacaga tattcgggca aactgatatt ggttgcttcc
     4381 cgcgcttcgg tattgggcag tttggcaaag tttgacttta cctggtttat tccggcggta
     4441 atcaaatacc gccggttgtt ttttgaagta ttggtggtgt cggtggtgtt gcagctgttt
     4501 gcgctgatta cgcctctgtt tttccaagtg gtgatggaca aggtgctggt acatcgggga
     4561 ttctctactt tggatgtggt gtcggtggct ttgttggtgg tgtcgctgtt tgagattgtg
     4621 ttgggcggtt tgcggacgta tctgtttgca catacgactt cacgtattga tgtggaattg
     4681 ggcgcgcgtt tgttccggca tctgctttcc ctgcctttat cctatttcga gcacagacga
     4741 gtgggtgata cggtggctcg ggtgcgggaa ttggagcaga ttcgcaattt cttgaccggt
     4801 caggcgctga cttcggtgtt ggatttggcg ttttcgttta tctttctggc ggtgatgtgg
     4861 tattacagct ccactctgac ttgggtggta ttggcttcgt tgcctgccta tgcgttttgg
     4921 tcggcattta tcagtccgat actgcggacg cgtctgaacg ataagttcgc gcgcaatgca
     4981 gacaaccagt cgtttttagt agaaagcatc actgcggtgg gtacggtaaa ggcgatggcg
     5041 gtggagccgc agatgacgca gcgttgggac aatcagttgg cggcttatgt ggcttcggga
     5101 tttcgggtaa cgaagttggc ggtggtcggc cagcaggggg tgcagctgat tcagaagctg
     5161 gtgacggtgg cgacgttgtg gattggcgca cggctggtaa ttgagggcaa gctgacggtg
     5221 gggcagctga ttgcgtttaa tatgctctcg ggacaggtgg cggcgcctgt tatccgtttg
     5281 gcgcagttgt ggcaggattt ccagcaggtg gggatttcgg tggcgcgttt gggggatatt
     5341 ctgaatgcgc cgaccgagaa tgcgtcttcg catttggctt tgcccgatat ccggggggag
     5401 attacgttcg aacatgtcga tttccgctat aaggcggacg gcaggctgat tttgcaggat
     5461 ttgaacctgc ggattcgggc gggggaagtg ctggggattg tgggacgttc ggggtcgggc
     5521 aaatccacac tcaccaaatt ggtgcagcgt ctgtatgtac cggagcaggg acgggtgttg
     5581 gtggacggca acgatttggc tttggccgct cctgcctggc tgcggcggca ggtcggcgtg
     5641 gtcttgcagg agaatgtgct gctcaaccgc agcatacgcg acaatatcgc gctgacggat
     5701 acgggtatgc cgctggaacg cattatcgaa gcagccaaac tggcgggcgc acacgagttt
     5761 attatggagc tgccggaagg ctacggcacc gtggtgggcg aacaaggggc cggcttgtcg
     5821 ggcggacagc ggcagcgtat tgcgattgcc cgcgcgttaa tcaccaatcc gcgcattctg
     5881 atttttgacg aagccaccag cgcgctggat tatgaaagtg aacgagcgat tatgcagaac
     5941 atgcaggcca tttgcgccaa ccggacggtg ctgattatcg cccaccgtct gtccaccgtt
     6001 aaaacggcac accgaatcat tgccatggat aaaggcagga ttgtggaagc gggaacacag
     6061 caggaattgc tggcgaagcc gaacggatat taccgctatc tgtatgattt acagaacggg
     6121 tagaaggagg ggatgggcgt gtaggctgct tttttcgtaa cgttctgcgt tttcagaccg
     6181 tcgtttactg ggtttactga ctgaaatgaa accaaagtgc aggctgcctg aaagactttt
     6241 tctgtctttt taggcagcct gcacttcttt ttcactcatc
//
LOCUS       DAXHNE010000112         6246 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.112, whole genome shotgun sequence.
ACCESSION   DAXHNE010000112 DAXHNE010000000
VERSION     DAXHNE010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.112"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            175..1233
                     /gene="alr"
                     /locus_tag="ACFM4Z_001598"
     CDS             175..1233
                     /gene="alr"
                     /locus_tag="ACFM4Z_001598"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFC6021608.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(1297..2652)
                     /locus_tag="ACFM4Z_001599"
     CDS             complement(1297..2652)
                     /locus_tag="ACFM4Z_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFC6021609.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAASKADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINVGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVKRGGRIPAPVQSMK
                     N"
     gene            complement(2667..2939)
                     /locus_tag="ACFM4Z_001600"
     CDS             complement(2667..2939)
                     /locus_tag="ACFM4Z_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFC6021610.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCSKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(3065..3802)
                     /locus_tag="ACFM4Z_001601"
     CDS             complement(3065..3802)
                     /locus_tag="ACFM4Z_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFC6021611.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGSIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHQRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(4083..4982)
                     /gene="prmB"
                     /locus_tag="ACFM4Z_001602"
     CDS             complement(4083..4982)
                     /gene="prmB"
                     /locus_tag="ACFM4Z_001602"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC6021612.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLTVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            5127..5720
                     /locus_tag="ACFM4Z_001603"
     CDS             5127..5720
                     /locus_tag="ACFM4Z_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021613.1"
                     /translation="MADGMGNLIGWEKTGLVAGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHATSKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPNIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDDHKHYDHFILKDE"
     gene            5832..>6246
                     /locus_tag="ACFM4Z_001604"
     CDS             5832..>6246
                     /locus_tag="ACFM4Z_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFC6021614.1"
                     /translation="MTKLYAEIAKMETQDDGTVKVWGYASSEEIDSDGEVIAAAAMKA
                     AIPDYMKFDAGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVSKVKTGVYKGFS
                     IGGSVTARDDLNKSQITGLKLTEISLVERPANPDAV"
BASE COUNT         1680 a         1577 c         1455 g         1534 t
ORIGIN      
        1 tttgggaaag cgattatatc aggaaaagca aaccgccttc ctacctgaaa actgctgctt
       61 cggcttgaag acacaagggt ctttaatatt ttaaaagcct tgccgttgga ttataattcc
      121 ctaattgatt tcttaatttt gctaataaac acttgtttgg taaggaatga atttatgcgc
      181 cctttgaacg tgcagatcag gttgggcaac cttaggcaca attatcggat tttaaaggaa
      241 atgcacggag gcaagctgtt ggcggtagtg aaggccgacg catacggaca cggtgcggtc
      301 agatgtgctt tcgcgctggc agacttggca gacggctttg ccgtggcgac aatcgatgaa
      361 ggaatcaggc tgcgggagag cggcattacc catccgattg tccttttgga aggcgtattt
      421 gaagcatcag agtacgaagc ggtcgaacaa tactcgctct ggccggcagt cggaaaccaa
      481 tggcaacttg aggctttgct ttcccgccat tggaaaaaac ctgtcaaggt ctggttgaaa
      541 atggattcgg gaatgcaccg taccggattt ttccctcatg attacgcttc ggcatatgcg
      601 gcattgaaac agtcggaata tgtggacagt attgtcaaat tctcgcattt ctcctgtgcg
      661 gacgaacccg aaagcggtat gacggaaata cagatggaag cattcgattt gggtacggaa
      721 gggctggaag gcgaagaaag ccttgccaat tcggcggcta ttttgaatgt tcccgaagca
      781 cgcagggatt gggggcgcgc gggcttggcg ttatacggca tttccccgtt cggaggcagc
      841 gatgacaggc tgaaacccgt gatgaggctt tcaacccgta tttttggcga acgcgtttta
      901 cagccgcact cccctatcgg ttatggcgca acattttata ccagcaaatc tacgcgcgtc
      961 ggcctgattg catgcggtta tgcggacggt tatccgcgcc gcgccccaag caattccccc
     1021 gtcgccgtcg acggtaaatt gacccgggtc atcggcaggg tctctatgga tatgatgacc
     1081 atcgagctgg atgcttcgca agaaggtttg ggacacgagg tcgaactgtg gggcgatacg
     1141 gtcaacatca atactgttgc cgaagcggcc ggaaccatcc cttacgaatt gatgtgcaat
     1201 atcaaacgtg caaaattcac ttatatcgag taatcgagtc caaacaaaaa tgccgtctga
     1261 agcctttcag acggcatttc cccatcaaaa ccgcaatcag tttttcatcg attggaccgg
     1321 agcgggaatt ctgccgcccc gtttgacgaa cacttcgcac gaaccttctt ttaccggcat
     1381 cacaggtgcg tagcccaaca ggccgccaaa ctcgacgctg tcgccgacgg ttttaccggt
     1441 taccggaata atgcgcacgg cagtggtttt actgttaatc ataccgatgg cggcttcgtc
     1501 ggcgataatg ccggaaatgg tgtgcgcggg tgtgtcgccg ggaacggcaa tcatatccaa
     1561 gccgaccgaa caaacggcgg tcatcgcttc gagtttatcc aacgtcagca cgcctgcttc
     1621 ggcggcggca atcatacctt cgtcttcgga aacggggata aacgcgccac tcaaaccgcc
     1681 aaccgcgctc gaagccatca tgccgccctt tttcacggca tcgttcagca atgccaaagc
     1741 tgctgttgtg ccgtgcgtac cgcagacgct caaacccatt tcttcaagaa tgcgcgccac
     1801 cgagtcgccg acggcagggg tcggtgccag cgacaagtcg agaataccaa acgggatatt
     1861 cagcattttt gaggcttcgc ggccgatgag ttcgcccacg cgggtaattt tgaaagcagt
     1921 tttcttcact acttccgcaa cttcggtcaa tgtcgttgca tctgaatttt ccaacgcggc
     1981 ttttacgaca cctgggccgg atacgccgac attgataacg gcatccgctt cgcccgaacc
     2041 atgaaacgcg cccgccataa acgggttgtc ttccaccgca ttgcagaaca cgacgatttt
     2101 cgcacagccg aaaccttcgg gcgtaatttc agccgtgcgt ttgatggttt cgcctgccag
     2161 cttgaccgca tccatattga taccggcacg cgtactgccg acattgatgg agctgcacac
     2221 gatgtcggtg gtcttcatcg cttcgggaat ggaacggatt aacacctcgt ctgaaggcga
     2281 catgcctttt tgcaccagcg cggaaaagcc gccgataaag gacacgccga tggctttggc
     2341 agccttgtcc aaagtctgcg ccacgctgac gtaagaatcg gctttgctcg ccgccgcgat
     2401 ttgggcaatc ggcgtgacag aaatgcgctg attcacaatc ggcactccat atttggcaga
     2461 cagatatttt gccgtcgcca ccaagtcttt gccgaccgtg gtgattttgt tgtaaatgtt
     2521 ttggtttaac acgtcgatgt cggtgctgat gcagtcgtgc aaatcaatgc cgatggtaat
     2581 ggtgcggaca tcgaaattct ggtcggcaac cattttgacg gtttctaaaa tttcgccgga
     2641 ttggatactc atcacattcc tccggctcaa atgcggtgca tcgcttggaa gatttcttcg
     2701 ttttgcatac ggatgtcgag cgcgagtttt ttgctctctt ccgcaaacaa atccaaaacc
     2761 tcctgccgcg atttgctgca ttttgaagtg tccaccaaga taatcatggt aaaaaaatca
     2821 tccatcagct gttggctgat attgaggatg ttgattcggt tttccgccaa aattttggaa
     2881 acatcgtaca cgatgccgac gcggtcttta ccgatgacgg tgatgactga attgttcaca
     2941 ggcttactcc ttgcagatat ccgttaaagt ccgaaattat accaccgttg gattttgaag
     3001 aaatattgtc aacaatatat acatacaaaa tgccgtctga aactatttca gacagcatca
     3061 agattcaggg ttcgattaaa taaccatcct tatcccactg ggttttcctg accaacttgt
     3121 catcctgata aacagcctcg ctctttttag aaccatcttc ataccactcc aaaaccaccc
     3181 cgttgcgttg atggtggcgg atagacagtt ccgagagtaa tcggccgctt tcatcccaag
     3241 tcagaatttt ggcaggctca tcgttgacca taaccatttc cgtcttgata ctgccgtcgg
     3301 cataccattg cttccatacg ccgtttgcct tattttgctt aaactggatt tcgctttcct
     3361 tgccgccgtt acggtaatag cggtaccccg taccttcact caaaccattt ttataaggca
     3421 taacggcaga ttttttaccg ttcggatacc agttgaccca ctccccgtcc ggcttaccct
     3481 tgctgaagcc ccccgccatt tttttctgac cattaaaatg ccacaaaatc aacataccgt
     3541 tttgcagggt aggcacaaaa gatttgattt gcgttgaagc aacgatataa ggttcggaat
     3601 atttcttcat cgacggataa taaaaatcct gcgcgtgcgc aataccctcc gccacactat
     3661 attgcctgat ataagcggca gaagacatcg tcgccgtcag tttcccgttc tgattaaaat
     3721 aaacagaata ggtctgcgcc ggcaaagcgg ccgaaaaacc caacaggaca gttgaaaata
     3781 caatccgaga taattttttc attgcaatag cgatataaaa acaaggctgt gttttagtaa
     3841 tcttttgatt tcaattattt gcaagggaaa agacaattat tttccggtta ggaataaacc
     3901 tattctattg aatatattga agccaagtac gcctatcaac actatattaa aacactgcca
     3961 aaaacaatta acttataaac aatatggtaa ggatttctct gccaagcatc aaacccgaga
     4021 caacgtatcg taaaaatgcc gtctgaaaac aaatcgtctt cagacggcat ttccccttca
     4081 actcactctt cacccaataa ctgctcgcgc gtcaagagga aaacaaaacc gtcgcccccg
     4141 ctggtttcca accaagtaaa aggcaactcc ggatacgctg cttccaatac atccctgtta
     4201 tgcccgattt ccaccagcaa tacacctttg ggattcagaa actttgccgc attcagaaga
     4261 atctgcctgg tggcatccaa cccgtccgcc ccgctgccca atgccaattc cggttcgtgc
     4321 aaatactctt caggcaataa ctcaaccgat tccgcatcca cataaggcgg attggaaaca
     4381 atcaaatcat aagtgccttc caatccttca aacaaatccg tatgaataag ccggatgcgt
     4441 tcttccaaac cataatcttc gacattaatc cctgccactt ccaaagcatc caagctcaca
     4501 tcaaccgcat caatttgggc atcaggataa tgatgcgcca tctgaatggc aaggcaaccg
     4561 cttccggtgc aaagatccaa agcattatgc accaactcat cgtattctat ccaaggacga
     4621 agtccgtcac ccaacaattc ataaataaaa gaacgaggta tgattacgcg ctcatccaca
     4681 tagaaatcaa actctccctg ccatgcctgg tgtgtcaaat aagcggctgg aatgtgttcg
     4741 acagcacgac gctcaataac cgtcagcact tcctcttttt cagcttccaa gagttttgca
     4801 tcaagatatg gggcaagcat atccaaaggc aaattcaaag tatgcagaat caaataagct
     4861 gcttcatcat gcgcattatc cgttccatga ccaaaaaaga gccctgcctc attaaaacgg
     4921 ctgactgcaa aacgtaaaac atcgcggata gtcgtcaatt cttgtgctgc ctgattaaac
     4981 ataatatgaa ccattctgcg tatagatact tttaattata acagaaacaa caagcaaacc
     5041 ttttcatatc gccaaataac cacccaatct acccatacaa ctacataaat gcccgcgcga
     5101 aaaccatcgc ccgaacggaa acgacaatgg ccgacggtat gggcaacctg attggctggg
     5161 aaaaaacggg gcttgttgcc ggtaagcagt ggataaccgc aaaagacgac aaggtgtccg
     5221 atgtctgcaa tgccaacggc gagatgggcg taatcgggct ttacgagcct ttctcacacg
     5281 gcgcattgac gatacccggt catccgaact gccgatgcga ggttgtttcc gtatcgggtg
     5341 gcgaattggg ggaatttgcc gaaaaaaagg agcttcgtaa agcggctatg cagtatgcgc
     5401 gggataactt tatcggcaaa agctatgtca atgaaaacag cgggcatgaa ctgaaggtaa
     5461 cttggcaagg tgtgaaacac gctacgtcaa aggcaaatca ggcggaatta tccatcatga
     5521 caaaacttga tgacttattg cgctacgcaa aatatgaggg ttcttattcg gataggaaag
     5581 gtcatcctaa tattattgca gcacataagt atcgtgccgt tgccaaggtt gggaatgagt
     5641 ctttaaatat cggtgtgatt gtaagggaat ttccagacga ccataaacat tacgaccatt
     5701 tcatcttgaa ggatgaataa agcccttttg cagtgtcgtt ctggagcgga tagcgttaag
     5761 gcaagtacac ttccagcctt gaaaaagggc tttaaattca gcatgccatt tatacaggca
     5821 ggagtaaacc catgacaaag ttatacgcag aaatcgccaa gatggagacg caggacgacg
     5881 gcacggtcaa ggtttggggt tacgcttcaa gcgaggagat cgattcggac ggcgaagtca
     5941 tcgcggcggc agctatgaag gcggcgattc ccgattatat gaagtttgac gcggggcgcg
     6001 agatgcacgg ctcaaacgct gcgggaacgg caattgaaat caacgttgaa gacgacggca
     6061 taaccttttt cggtgctcat atcatcgacc ccgttgtcgt gtcgaaagtc aaaacaggcg
     6121 tttacaaagg cttttccatc ggcggcagcg ttaccgcccg cgatgatttg aacaagtcgc
     6181 aaatcacggg cttgaagctg acggaaatca gccttgtcga gcgccctgcc aatcccgatg
     6241 cggtgt
//
LOCUS       DAXHNE010000113         6244 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.113, whole genome shotgun sequence.
ACCESSION   DAXHNE010000113 DAXHNE010000000
VERSION     DAXHNE010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.113"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..129)
                     /locus_tag="ACFM4Z_001605"
     CDS             complement(<1..129)
                     /locus_tag="ACFM4Z_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HFC6021615.1"
                     /translation="MLKTLTFAALHFSTAFSVTYVLTGSIGISGAVALVEPLINTVV"
     gene            251..1204
                     /locus_tag="ACFM4Z_001606"
     CDS             251..1204
                     /locus_tag="ACFM4Z_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HFC6021616.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRD
                     AHIVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHASAVREGYVSFEDAVRAADVLSLHCPLNAQTENMIGEN
                     ELRQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTEEPPRGGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(1279..3336)
                     /gene="metG"
                     /locus_tag="ACFM4Z_001607"
     CDS             complement(1279..3336)
                     /gene="metG"
                     /locus_tag="ACFM4Z_001607"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFC6021617.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECH
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFFIGYDNYYSTHSPENKQ
                     FSQDIYRALKAGGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQAE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CGRIGIDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRLKDVADSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADAVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AAKFLNLEAITWANTRDTLGEHAINKYEHLMQRVEQKQVDDLIEANKQSIAAAAVPAA
                     EEGKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     AFYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGD
                     KVG"
     gene            complement(3452..5290)
                     /gene="glmS"
                     /locus_tag="ACFM4Z_001608"
     CDS             complement(3452..5290)
                     /gene="glmS"
                     /locus_tag="ACFM4Z_001608"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFC6021618.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GSFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGELSPVVHTIPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            5572..6102
                     /locus_tag="ACFM4Z_001609"
     CDS             5572..6102
                     /locus_tag="ACFM4Z_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021619.1"
                     /translation="MKQMLLAVGVVAVLAGCGKDAGGYEGYWREKSDKKEGVIAVKKE
                     KGNYFLNKINVFTGKEESLLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTVQAYRDARNALPSNQTYQQRQAAIEQLKRRFEAEFDELEK
                     EIKCNGRPGHCCFSGG"
BASE COUNT         1321 a         1493 c         1903 g         1527 t
ORIGIN      
        1 aacgacggtg ttgattaggg gttcgaccag cgcgaccgcc ccggatatgc ctatactgcc
       61 cgtcagtaca taggttacac tgaaggcggt gctgaaatgc agtgcggcaa aagtcagggt
      121 tttaagcatc atcctctccc gaattggaca ttgacggagg aatgataaag gttatcataa
      181 ggttgcgcgg tttaaatttg ctatttattg ttagcgtaaa taaatcattt tccaaataag
      241 gatagaaatt atgaatcata aaaagatcgt tgttttggat gcggatactt tgcccggtcg
      301 ggtctttcat tttgattttc cgcacgagct tgcggtttac ggtacgacag gtgcggatga
      361 aacggcggaa cgggtgcgcg acgcacatat tgtcattacc aacaaagtga tgatttctgc
      421 cgatattatt gcggctaatc cgcagttgga gctgattgcc gtcagtgcga ccggtgtgaa
      481 caatgtcgat attggggcgg cgaaggcggc gggcgttgcg gtatgcaatg tccgcgccta
      541 cggaaacgaa tcggttgcgg aacacgcctt tatgctgatg attgcgttga tgcggaattt
      601 gcccgcctat cagcgtgatg ttgcggcagg attatgggaa aagtcgccgt ttttctgcca
      661 ttacggtgcg ccgattcggg atttgaacgg caaaacgctg gcggttttcg gacgcggcaa
      721 tatcggacgg acgcttgccg gatacgcgca ggcattcggt atgggggtgg tgtttgccga
      781 acacaaacac gcgtccgctg tgcgtgaagg ctatgtttcc tttgaagatg cggtacgggc
      841 tgctgatgtg ttgtcgctgc actgtccgct aaacgcccaa actgaaaata tgataggcga
      901 aaacgaattg cggcagatga agcctggcgc ggttttaatc aattgtgggc gcggcgggct
      961 ggtggatgaa aacgcgctgc ttgccgcact caaatacggg cagatcggcg gggctggtgt
     1021 cgatgttttg acggaggagc cgccaagggg cggcaatccc ctgctgaatg cacgacttcc
     1081 caatctgatt gttacgccgc ataccgcgtg ggcgagccgc gaggctttgg acaggctgtt
     1141 cgatatattg ttggcgaaca ttcacgcctt tgtgaaaggc gaggcgcaaa accgggtagt
     1201 ttgagcttgt cgggataacg aaaaacaatg ccgtctgaac gcccaaaggg ttcagacggc
     1261 attttttgag atttctactt aaccgacttt gtcgcccggt tgcgcgcctg catccacatc
     1321 caagagcttc agtttcccgt ctgccgtggc ggcactcaaa atcatgcctt cggatacgcc
     1381 gaattttgcc attttgcgcg gggcgaagtt ggcgacggcg atgaccattc ttccgtttaa
     1441 ctcggcaggg ttggggtaaa acgcggcgat gccggagaag atgatacgtt tttcaaaacc
     1501 gaaatcgagg tcgaatttta agagtttggt gctgccttcg acggcttcgc agttcaatac
     1561 tttggcgacg cgcatgtcga ttttcataaa gtcgtcgaaa ctcgcctgtt cggcaacttt
     1621 ttcgtatttg ccctcttcgg cggcaggcac ggctgcggcg gcgatgcttt gtttgttggc
     1681 ttcgattaaa tcgtccactt gtttttgctc cactcgttgc atgagatgtt cgtacttgtt
     1741 gatggcgtgt tcgcccaagg tatcgcgtgt atttgcccaa gtgatggctt ccaaattcag
     1801 gaatttggcg gcgtttgcgg cggtttgcgg caagacgggg gcgaggtagg cggtcaacat
     1861 ggtgaaggcg ttgatgagct cgctgcacac ttcgtgcagg cgttcgtctt gaccttcctg
     1921 tttcgccagt tcccaaggtt tgttggcatc aacgtattcg ttcaccgcat cagccaaagc
     1981 cataatgtcg cgcagggcgc gggcgtattc gcggttttcg tattgctcgg cgatggtgtc
     2041 gctttcggct gcaagttttg ccagcaatgc gctgtcggca acgtctttca gacggccttc
     2101 aaagcgtttg gcgataaaac ctgaggcgcg ggcggcgatg ttgacgtatt tgcctacgag
     2161 gtcgctgttg acgcgcgaga taaagtcttg caggttcaaa tcgatgtctt cgattttgct
     2221 gttgagtttt gcggcgatgt agtagcgcat ccactcgggg tttaaacctt gttccagata
     2281 ggatttggcg gtgataaacg tgccgcgcga tttggacatt ttttgtccgt ctacggtcaa
     2341 aaagccgtgt gcgtacacgc cggtcggggc gcggtggccg gagaaatgca gcatagcggg
     2401 ccagaacagg gcgtgaaaat agaggatgtc tttgccgatg aagtggtaca tctcggtttg
     2461 gctgtcggct ttgaagtatt cgtcgaaatc gatgccgatg cggccgcaca ggtttttaaa
     2521 cgatgccatg tagccgacgg gcgcgtccag ccagacgtag aagtatttgc ccggcgcgtc
     2581 ggggatttca aaaccgaaat acggcgcgtc gcgggaaatg tcccagtcgg acagcgtggt
     2641 ttcttcgcct tcgcccagcc attctttcat tttgttgagg gcttcggctt gcagatgggg
     2701 cttgccgtcg tgcgggttgt tgccggaagt ccatgctttg aggaagtcgg cgcattcgcc
     2761 cagtttgaag aagaagtgtt cggattcgcg caattcgggt ttcgcgccgg aaacggcgga
     2821 atacgggttg atcagttcgg tcggggaata ggtcgtgccg cagacttcgc agttgtcgcc
     2881 gtattggtct tgggcgtggc atttggggca ttcgcctttg acgaagcggt cgggcaggaa
     2941 catttgcttt tcaggatcga aaagctgctc gatgacgcgg ctttcgattt tgccgccggc
     3001 tttcaacgcg cggtaaatgt cttgggaaaa ctgtttgttt tcaggggaat gggtgctgta
     3061 ataattgtcg tagccgatga aaaagccggt gaagtcggcg aggtgttctt cgcgcacttt
     3121 ggcaatcatg tcttcgggcg cgataccttg tttttgcgcg gcaagcatta cgggcgtgcc
     3181 gtgggtgtcg tcggcgcagc agtagtggca ctcgtggccg cgcagttttt gaaagcgcac
     3241 ccaaacgtcg gtttggatgt gttcgaccat gtggccgagg tggatgctgc cgttggcata
     3301 gggcagggcg gaggtaacta agattttgcg tgtcatattg tgctttgcaa acaatgggta
     3361 aaggcggatt ataccgcaaa tcaaacgggg aaatgccgtc tgaagcctga aaaatcaggc
     3421 ttcagacggc atttttgcca accggcggga tttattcgac ggttacggat tttgccagat
     3481 ttcgcggttt gtccacatcc gtgcctcggg cgagggcggt gtggtaggcg agaagctgca
     3541 cggggatagt atgcacgacg ggggacagtt cgccgacatg gcgcggcgca cggataacgt
     3601 gcacgccttc ggtggcatta aaattgctgt cgaggtcggc aaagacgaaa agttcgccgc
     3661 cgcgcgcgcc gacttcctgc atattggctt tgactttgtc caacaggctg tcgttgggcg
     3721 cgatgacgac gacgggcata ttttcgtcca ccagggcaag cggcccgtgt ttcagctcgc
     3781 cggcaggata ggcttcggcg tggatgtagg tgatttcctt cagcttcaac gcaccctcga
     3841 gggcaatcgg gtaatggatg ccgcgcccta aaaacagcgc gctggttttc ttggcaaact
     3901 gttgcgccca ggcggcaatt tgcggttcga ggttcaaagc gtgctgcacg ctgccgggaa
     3961 gctggcggag ttcttcggta taacgtgctt catcttcgcc ggataccaag ccgcgcactt
     4021 tcgccagcgt taccgccaaa ccgaacagcg cgaccagctg cgtggtaaat gccttggtcg
     4081 aggcgacgcc gatttccgca ccggcacggg tataaagcac gaggctgctt tcgcgcggca
     4141 gggcggattc catcacgttg caaatggaga ggctgtggcg gtgtcccaaa gatttggcgt
     4201 atttcaaagc ctccatcgtg tccagcgttt cgccggattg ggaaatggta atgaccagtt
     4261 ggtcgggatc ggcaatcacg ctgcggtatc ggtattcgct ggcgatttcg acgtcagccg
     4321 ggattttggc gatggattcg agccagtatt ttgcggtcag cgcggcgtaa taggacgtgc
     4381 cgcaggcaag gattttgacg ctgcggatgc tttcaaacac gcttttggcg tttttgccga
     4441 agttttcggg gatgaagctg ccgtcgagga aaacttccgc cgtatccgca atcgcgcgcg
     4501 gctgctcgtg gatttccttt tgcataaagt ggctgtacgg tcccagttcc aaagaggcga
     4561 gcgagagttc ggataccttg actttgcgtt cggcaggcag gccgttttta tcggtcagcc
     4621 gtttgatgcc gtctgaagcc agcagcgcga tgtcgccgtc ttcaaggtac gccacgcggc
     4681 gcgtaaaggc gatgacggcg gatacgtccg aagcgataaa ggtttcatcg tcgcccaaag
     4741 cgaccaaaag cgggcagccc atacgcgcca caactaattc atcaggcttg tcttgggcaa
     4801 taaccgcgat ggcgtatgcg ccgtggaaac gtttgaccgc ttcctgcacg gcttcaaaca
     4861 gcctgccgcc gttttgcgcg tattcgtgat tgatgctgtg tgcgatgact tcggtatccg
     4921 tttgcgattc aaaacggtat cccaaacctt ccaagcgttt gcgttcgctt tcaaagtttt
     4981 cgatgatgcc gttgtggacg accgcaatca tcccgccgct gatgtgcggg tgggcgttcg
     5041 gctcggtaac gccgccgtgt gtcgcccagc gcgtatgccc gatgccgatg ccgccgctga
     5101 tgcctttttc gcgtgccgcg tcttccataa gctgcacgcg tccgacgcgg cgcacgcgtt
     5161 tgattttgcc gtcggtgttg acggcaatgc ctgatgagtc ataaccccgg tattcgaggc
     5221 gtttgaggcc gtcggtcaga aaatcgacga cgttgtgatg ggcgcggatg gcgccgacga
     5281 taccgcacat aactgttcct tagtatccgg ttgaaaaaaa acaggcgcgg acggctcccg
     5341 tgccgaacct tcctcttcgg attataaacc gcctcccgcg ccgaaaaaca gcaaaatgcc
     5401 gtctgaaggc ttgggcttgc tcaaaaaagg gagggatttc cccgcctgac cgggatgggc
     5461 gttcagacgg cattgcctgc cgccggttta tagtttttac aaatcaatat tgacaagctg
     5521 aaaaaaaaaa acaatatact cgctcggtct taatgttaac ggagtataga aatgaaacaa
     5581 atgcttttag ccgtcggcgt ggtggcggtg ttggcgggct gcggcaagga tgccggcggt
     5641 tacgagggtt attggcgcga aaagtcggac aaaaaagagg gcgtgattgc cgtcaaaaaa
     5701 gaaaaaggca attacttcct taataaaatc aacgtattta caggcaagga ggagtctttg
     5761 cttttgtctg aaaaagacgg cgcgctttcg ataaacacag ggatagggga aatcccgatc
     5821 aaactttccg acgacgggaa agagctgtat gtcgaacgca ggcagtatgt gaaaaccgat
     5881 gcggcgatga aggacaaaat catcgcccac cagaaaaagt gcggacaaac agtacaggca
     5941 taccgcgacg cgcgaaatgc gttgccgtca aaccaaacgt atcagcagcg tcaggcggcg
     6001 atcgagcaat tgaaacggcg gtttgaagcc gagttcgatg aattggaaaa agaaatcaaa
     6061 tgcaacggca gacccgggca ttgttgcttt agcggaggat aaccgggagg atgccgccgt
     6121 ccgaatcgga cgtgcggttt ccgtaccggt acaggcggac gggaatgtcc gccttttttt
     6181 gttcggacgc gtttgaatat ccgtccgatt ccgtccgttt gcaaagggta tttttgttcg
     6241 ggcg
//
LOCUS       DAXHNE010000114         6174 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.114, whole genome shotgun sequence.
ACCESSION   DAXHNE010000114 DAXHNE010000000
VERSION     DAXHNE010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.114"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(1094..3841)
                     /gene="tbpA"
                     /locus_tag="ACFM4Z_001610"
     CDS             complement(1094..3841)
                     /gene="tbpA"
                     /locus_tag="ACFM4Z_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HFC6021620.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEAAGRGVQSFNRLVPVEDTNQYANFIVEEECESGNYETCKANP
                     KKDVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILERT
                     QQTFDTRDMTVPAFLTKAVFDENKKQAGSLSGNGKYAGDHKYGGLFTNGENGALVGAE
                     YGTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADG
                     SDKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGS
                     NLRHQDYYYQNANRAYSSNLPTQNGNQNISPNGSQDKPYWVSIGGGNVVTGQICRLGN
                     NTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTL
                     SWNAGVVLKPFTWMDLTYRTSTGFRLPSFSEMYGWRSGDKIKAVKIDPEKSFNKEAGI
                     VFKGDFGNLEASWFNNAYRDLIVRGYEAQIKNGKEEAKGDPAYLNAQSARITGINILG
                     KIDWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYD
                     QPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTI
                     KKHFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMK
                     F"
     gene            complement(3928..6057)
                     /locus_tag="ACFM4Z_001611"
     CDS             complement(3928..6057)
                     /locus_tag="ACFM4Z_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6021621.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKPQAQKDQGGYGFAMRLKRRNRHPQAKEDKVELNPNDWEETGLPSKPQNLPE
                     RQQSVIDKVKTDDGSNIYTSPYLTQSNHQNGSTNSGANQPKNEVKDYKNFKYVYSGWF
                     YKHAESEREFSKIKFKSGDDGYIFYHGKDPSRQLPTSEKVIYKGVWHFVTDTEKEQKF
                     NDILETSKGQGDRYSGFSGDDGETTSNRTDSNLNDKHEGYGFTSNLEVDFGSKKLTGK
                     LIRNNRVTNAPTNDKYTTQYYSLDAQITGNRFNGKAIATDKPDTGGTKLHPFVSDSSS
                     LSGGFFGPKGEELGFRFLSDDKKVAVVGSAKTKDKTENGAVASGGTDAAASNGAAGTS
                     SENSKLTTVLDAVELKLGDKEVQKLDNFSNAAQLVVDGIMIPLLPEASESGNNQANQG
                     TNGGTAFTYKTTYTPKSDEKDTQAGTAANGDQAASNTAGDTNGKTKTYEVEVCCSNLN
                     YLKYGLLTRKTAGNTGEGGNGSPTAAQTAQGAQSMFLQGERTDENKIPSEQNVVYRGS
                     WYGHIANGTSWSGNASNATSGNKADFTVNFGEKKITGTLTAENRQQATFTIEGMIQGN
                     GFSGTAKTADSGFDLDQSNTTGTPKAYITNAKVQGGFYGPKAEELGGWFAYPGDKQAQ
                     PSASGSGASAANSATVVFGAKRQQLVQ"
BASE COUNT         1319 a         1570 c         1543 g         1742 t
ORIGIN      
        1 tcgttcccac ggtcaggatt tcagattgcg gacatctgtc agaaaagaca aaaaaccttc
       61 cgccgtcatt ccctacaggc gagaatccga tccgttgaaa ttcggttgtt ttaaataaat
      121 tcttgcagct ttgattttct gtttttccga taacgccgta actttgaaac gcgaaagcgg
      181 taatccgatc cgttgggatt ttgcaacttc aaatcaatcc gcaaactgaa atcccgtcat
      241 tcccgcgcag tcgtgaatcc gaacgcgtcc gcacgaaaac ctgcatcccg tcattcccgc
      301 gcagtcgtga atccgaacgc gtccgcacga aaacctgcat cccgtcattc ccacggaagt
      361 gggaatctag gacgtaaaat ctaaagaaac cgttttatcc gataagtttc cgcaccgaca
      421 gacctggatt cccgcctgcg cgggaatgac gaaatttcgg cgagccgtag ggtgggctgt
      481 agggtgggct tcagcccacc aatttcaccg catcaaaggt ttttgggaat acggcggttt
      541 cggttttcgg tgggaatggg cggaatggat aacattgacg gagttggggg aataggtgga
      601 aacggtggga ttggtgggct gaagcccacc ctacagccag ccctatgtcc acgctgcaca
      661 tcctacgccc gccttgcgtt tcttataagc agcattttac gcaatacttt gcgtccgctg
      721 aaaaaatttc aaacggtctt ttttatcaaa tgcgaaaaat ataacgtcat tcccgcgcag
      781 gcgggaatct agtctgtcgg tgcggaaact tatcggataa aacggtttct ttagatttta
      841 cgccctagat tcccgcctgc gcgggaatga cgggatttga ggtttctgtt tttgattttc
      901 tgttttcgta ggaatgacgg tttagagtta cccgaaacct caaaaaaacc taaaccgaac
      961 ggactggatt cccgcctgct cgggaatgac ggatttcagg attacggtgt atcggggatg
     1021 atgggaaacg gtgggaattg tgtaaaaaat gccgtctgaa gcctttcaga cggcatttgc
     1081 ggcgtttgga cgtttaaaac ttcatttcca agctaaatgt gtagttgcga ccgggggcgg
     1141 catatcggtt gtaaacgccg acatttttgt gttggttgac tgcgccgccg gcagtttgcc
     1201 gcacattttc ccaagtaaca tagcggtagt tgaggaggtt gtacacgccc gcacggaggg
     1261 taaagtgttt tttaatcgtg taataaccgg acacatccac aatataccaa gggcgggtac
     1321 ggcgcgcggt ggcttttgta ttgcggctgt tgccgttgag caaagcccgg ctgcccaaca
     1381 actctgtgat ttccttggct ttggaataag tcagcatacc gttcacaccc catttgcctt
     1441 ccggttggtc atagcccaag ccgacgacat agcgcgaggg ttggatggca tcaaacaaat
     1501 gtgattgaat atcggtgcgg tctgcgcgtt ttttaatatc tcggacacgg acacgattat
     1561 aggcaaatgt ggaataccat ccttcgggca atttatccca tacgccgttc caatcgattt
     1621 tgcccaaaat attgatgccg gtaatccgcg cgctttgggc attgaggtaa gccgggtcgc
     1681 ctttggcttc ttctttgccg tttttaattt gcgcttcata accccggaca atcaaatcgc
     1741 ggtaggcatt gttgaaccaa cttgcctcca agttgccgaa atcgccttta aacacgatgc
     1801 cggcttcttt gttgaacgat ttttccggat cgattttgac ggcttttatt ttatcgcccg
     1861 accgccagcc gtacatttcc gaaaacgagg gcaggcggaa gccggttgaa gtgcggtaag
     1921 tcaaatccat ccaagtgaaa ggtttgagga ctacacccgc gttccaagac agggtgcggt
     1981 gcgtgccggt ggaaacgctg ccgtcgtccg aatgcgtgct gcggtagtcg tagcgcaacc
     2041 ccgcgccgac atccgcccac ctgcccaaac ggacattgtc ccgaactgcc gcataatagc
     2101 ttttgccgtt gatgctgcgc ggcgtgcagt ccgtataagt attattgccc aagcggcaaa
     2161 tttgccccgt aaccacattt cccccgccta tgctgaccca atagggtttg tcttggctgc
     2221 cgttggggct gatgttttga ttgccgtttt gagtgggcag attcgacgaa taggcgcggt
     2281 tggcattttg ataataataa tcctgatggc ggagattaga gccaaagcgg tcaaaaccga
     2341 gattcacgct caggttgtgg cggattttgg cggtatcgaa ggattttttg aatgccgcct
     2401 gcaagagcct gtggctttcc ccgtaaatca cgcgatcgga tttgtagtag gaaaacggct
     2461 tgtcggcact cggacggcaa tatttgtccg aaccgtcggc agaacagtgc gtctgctgaa
     2521 aatgattgtc caaaccgatg ccctgacggt cgtaagagag gcgggcataa tctgcccaag
     2581 tgtctttatc ggcattggta tagacatatt ccaaaccgta gcggcttttg gtgtgcgtct
     2641 cgtcgtaaaa cacgcccgta ccgtattccg cgcccaccag cgcaccgttt tcgccgttgg
     2701 taaacagtcc gccgtatttg tggtcgcccg cgtatttgcc gttgccggac aaagaacccg
     2761 cctgtttttt attttcatca aaaaccgcct tggtcaggaa tgccggaacc gtcatatcgc
     2821 gcgtgtcgaa agtttgttgc gtgcgttcga gtataccgcc gatatagtgc cgcttattct
     2881 caaaacgaaa acccgggcgg aacagccacg accggctttc gtatgaaagc ggatcggcga
     2941 gaaagcggtt gggacccgtg tagtctcggg tagaaaccgt ttgacgttcg tctttgccga
     3001 caacatcttt tttcggattc gctttacacg tttcgtaatt cccgctttcg cattcttctt
     3061 caacgatgaa atttgcgtac tgattggtat cctcaaccgg caccagcctg ttaaagctct
     3121 gaacgccgcg tccggcggct tcgtgggcgc ggatttcccc cgcgcgccga cccgtgtgga
     3181 tcagcaaagc ctccgcaccg ccgatgcgcc ccgccagcgc gatggattgg gtaagccccc
     3241 ggtttttgcc ggaataggcg gttttactct gaatgcccca ctgcctgcct tccccgataa
     3301 catcgtcggc ggttttggtt tgaaatgcga ccgaacccgc caatgcgccg ctgccttgtt
     3361 cgaccgagtt tgagcctttg ctgatttcga cagccttgac gttttcatac tcgatttcat
     3421 tgattgcgcc gctgctgccc gccgtcctcg tcccgcccaa tgccgcctgc gcggtgtagg
     3481 actgtatttg cgccaagccg tccaccgtca aggaaacgcg gtttttgtcc ataccgcgta
     3541 tcgaatagcc cgagcttgcg ccacgcccct gttcgaccac ggcgatgccg gggtcgtaac
     3601 gcgtcaggtc gcggatatcg agtacctgtt ccttgctgag ggtgtcggcg gttttgacca
     3661 atttgcccaa accggttact tcgttatccc ggcgggtttt ctgttttttg gcttttacct
     3721 gtatggtatc caactgtttt tcctgtgctt gtccggcttg cacattttct gcataaacgg
     3781 gcagcgcagt cattaaagac aggcataaaa tatttaatcg gaacaaatgt tgctgttgca
     3841 tagtgtttcc ctactcttcg ctttcagacg gcatcggaag gagcgatgcc gtctgaagcc
     3901 ttattctcga ttgttcggca gccgtgctta ttgcacaagc tgttggcgtt tcgcaccgaa
     3961 taccacggtc gcgctgttgg cggctgatgc gcctgacccc gaagcggacg gctgcgcctg
     4021 cttgtcgccc ggataggcaa accatccgcc caactcttcg gctttgggcc cgtaaaaacc
     4081 gccctgcacc ttggcgtttg tgatatatgc cttaggcgta ccggtggtat tgctctgatc
     4141 gagatcaaag cctgagtcag cagtttttgc cgtaccggaa aaaccgttgc cctgaatcat
     4201 tccctcaatg gtaaaggttg cctgctgcct gttttcagcg gttaacgtgc cggtaatttt
     4261 tttctcgcca aaattcacgg taaagtccgc cttgttgcca ctcgttgcat tggaagcatt
     4321 gccgctccag cttgtgccgt tggcaatatg cccgtaccaa gacccccgat aaacgacgtt
     4381 ttgctcgctt ggaatcttgt tttcatcggt gcgctcgcct tggaggaaca tactttgtgc
     4441 accctgtgcc gtttgggcgg cggttgggct gccgttgccg ccttcccccg tattgccggc
     4501 agtcttgcgc gtcagcaacc cgtatttcag ataattgagg ttggaacagc agacttcgac
     4561 ttcataggtt tttgttttgc cattggtatc acctgccgta tttgaagcgg cttgatcgcc
     4621 attcgccgcc gtacctgctt gggtgtcttt ttcatcgctt ttcggcgtgt aggttgtttt
     4681 gtaggtaaag gctgttccgc cgtttgtacc ttgattggct tgattgttcc cactttcgga
     4741 agcctcgggc aggagcggaa tcataatgcc gtcgacaacc agttgggcgg cgttgctgaa
     4801 gttgtcgagc ttttggactt ccttatcgcc caatttcagc tcgaccgcat ccaaaaccgt
     4861 ggtcagctta ctgttttcag acgacgtgcc tgccgcaccg tttgatgctg ccgcatctgt
     4921 gccgcctgaa gccaccgcgc cattttccgt tttgtctttg gttttcgcgc tgccgacaac
     4981 cgcaactttt ttatcgtcgc tcaaaaagcg gaaacccaat tcctcaccct tcggaccgaa
     5041 aaagccgccg ctcaaagaag acgagtcgga aacaaaggga tgtagtttgg ttcctccagt
     5101 gtcgggtttg tcggtcgcta tcgccttacc gttgaagcgg ttgcctgtta tttgggcatc
     5161 aaggctgtag tattgggtgg tgtatttatc gttagtagga gcgtttgtaa ctctattatt
     5221 gcgtattaat ttacccgtca attttttact gccgaaatcc acttctaaat tcgaggtaaa
     5281 accataaccc tcgtgcttat cattaaggtt ggaatcagtt ctattggaag ttgtttcgcc
     5341 gtcatcgccc gaaaatccgc tgtatctgtc gccttgccct tttgaggttt caagaatatc
     5401 gttaaatttt tgttcctttt cagtatcggt tacaaaatgc catacgcctt tgtagataac
     5461 tttttcagaa gtgggaagtt gtcgggaagg gtctttaccg tgataaaaaa tatagccgtc
     5521 gtcgcctgac ttaaatttga ttttactgaa ttctctttca ctctctgcat gtttataaaa
     5581 ccagccggaa taaacatatt tgaaattttt gtaatctttt acttcgtttt ttggttggtt
     5641 tgcaccgcta ttagtgctgc cgttttgatg gtttgattgc gtgagataag gggaagtgta
     5701 aatattgctg ccatcgtctg tttttacttt atcaataacc gattgctgtc gctcgggtaa
     5761 gttttggggc ttgctcggca atcctgtctc ctcccaatca tttgggttta gttcaacttt
     5821 gtcttctttt gcctgcggat gccgattcct ccgtttcaac ctcatcgcaa aaccgtatcc
     5881 gccttggtct ttttgggctt gcggtttttc ggaagaaaca tcttgatact ttggcgcggg
     5941 acgcggggct tcggtatcga cagaatcaag atcgaaactg ccgcctccgc ccaaacaagc
     6001 actcaacaaa aacacaggca gcaccatagc agcctgattt accaatggat tgttcatata
     6061 aacccaattc aattaaagaa tgataaggat tattatttta tttattttta acaaatttgc
     6121 aaatactttt ttattttttt agggaataca ccaaaatccc gtcattccca cgaa
//
LOCUS       DAXHNE010000115         6165 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.115, whole genome shotgun sequence.
ACCESSION   DAXHNE010000115 DAXHNE010000000
VERSION     DAXHNE010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.115"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(64..624)
                     /gene="sodC"
                     /locus_tag="ACFM4Z_001612"
     CDS             complement(64..624)
                     /gene="sodC"
                     /locus_tag="ACFM4Z_001612"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFC6021622.1"
                     /translation="MNMKTLLALAVSAVCSVGVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
     gene            complement(1041..2081)
                     /gene="mutY"
                     /locus_tag="ACFM4Z_001613"
     CDS             complement(1041..2081)
                     /gene="mutY"
                     /locus_tag="ACFM4Z_001613"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFC6021623.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLLITPFEAQMPSESPSDGIWIKPAHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            2244..2444
                     /locus_tag="ACFM4Z_001614"
     CDS             2244..2444
                     /locus_tag="ACFM4Z_001614"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFC6021624.1"
                     /translation="MKAMVYHGANDIRFEEKPRPQIIDPTDAVVKIVKTTICGTDLGI
                     WKGKKNPKSPTAVFSVMKASVL"
     gene            2375..3175
                     /locus_tag="ACFM4Z_001615"
     CDS             2375..3175
                     /locus_tag="ACFM4Z_001615"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFC6021625.1"
                     /translation="MERQKKPEVADGRILGHEGIGIVEEVGEAVKNIKVGDKVIISCV
                     SKCCTCDNCKIQLYSHCRNGGWILGYMIDGTQAEYVRNSLVPLPDNVNEEVALLLSDA
                     LPTAQLYSPAAIIVCDMDENRLKLAKELGATHTISPASGDVSKQVFAIVGEDGVDCAI
                     EAVGIPATWNMCQDIVKPDGHIAVVGVHVQSVDFKLEKLWIKNLAITTGLVNANTTEM
                     LMKAISSSSVDYTKMLTHRFKFSELEKAYDVFKHAAENQAMKVVLEAD"
     gene            complement(3431..4069)
                     /locus_tag="ACFM4Z_001616"
     CDS             complement(3431..4069)
                     /locus_tag="ACFM4Z_001616"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFC6021626.1"
                     /translation="MSKLTPREILSAGAVVPVMAIDDLSTAVDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAS
                     HNSGIPLIPGVATPGEVQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(4250..6085)
                     /gene="edd"
                     /locus_tag="ACFM4Z_001617"
     CDS             complement(4250..6085)
                     /gene="edd"
                     /locus_tag="ACFM4Z_001617"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFC6021627.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAIGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLDAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKAGNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVSGELNVLINETE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
BASE COUNT         1371 a         1474 c         1733 g         1587 t
ORIGIN      
        1 gctaaatatc tagcaaaaaa gtgcggtcaa aattcaccgc acttttcgtt tcgaacaatc
       61 gaattattta atcacgccac atgccatacg tgggccgcca ccgccaagtg gagctggatg
      121 atcggagtga ttatcaccac ccgtgtggat cataatagag tgaccgcgaa catcatctaa
      181 atgtttaaga cgtggtgcta aaacaggatt tgttgctgtg ccatcatgca atacagttaa
      241 tgcaggtaaa tcacctaagt gtgcatcatc ttgccatggg taaccatgtt gttttgcacc
      301 tttaggatcc cagtgaccgc ctgcgcctaa accagctgtc aatttacctt ctttttcttt
      361 tggctcacag cttgggtttt catggatgtg gaaaccatgt aagccttcgc ttaatccttg
      421 taaatcaggg gtaaacacaa gaccatagtt agattcagta atagtcactg tacccacatc
      481 tttgttaccg tttactggat caagttgttg cactttcact tcaatagaag cacctttagg
      541 tatcgtatta tgctcgtgtg cttgcgcaac accaactgaa catactgcac taaccgctag
      601 tgctaataag gttttcatat tcattgcaat ctcctttatt gcagtaaaaa ctcgcgacta
      661 ttttagtcaa ttttgtttta aaagccgata aatttttacg aaaattttac attaattgtt
      721 atttgcagaa aattagaacc cgttatcacg tcttggataa atgccaaaac cattgtgatt
      781 ccgagaatta aaaagaaaaa tttcggaatc ctcatccttt tctgaaacct gattgcggta
      841 tccatggctt tgtctttgtc atttaagttt gtgttcatgt ttttgacctt ctttgctttt
      901 taaatccttt ttaaattgcc gacttcttcc gttccaaatg tcccatttga acattatccc
      961 gccgcctaaa atacgaccca aattcatcgg ttgttcttgc tgcgatacct gcaaacaaca
     1021 gttcaatggg tttattttgt ttattctaac ctattaccgt ttaaataaat ttccaaaggc
     1081 ttgggcaaac cgtaatcttt caaatgcgcc ggctttatcc aaatgccgtc tgaagggctt
     1141 tcagacggca tttgtgcttc aaagggcgta atcaatagca gccggtgcgt caggcggtgg
     1201 gtcagggcgg tttgttcgtc catatctgcc atggtcaggg agaatttggc ggcaaagtcg
     1261 gaaagcccgt tcaaactttc aaaacacggc acgcaataca gcccgcccca aatgcctttg
     1321 gcggggcgtt tttccagcaa aatcgcgccg tcccggttgc ggacaatcag ccagtaaagc
     1381 ggcagggtcg gtacttcggc ggcggttttt ttgcgcggca gctcggcggt gcggttttgc
     1441 tttttcgctt cgcagatgtc cgccatcggg cattggtggc acaagggttt cgtccgtttg
     1501 cacacggtcg cgcccaaatc catcaaacct tgtgtatagg caggcatatc ggcgttttca
     1561 gacggcagca ggctttcggc aagtgtccag agcgagtttt caaatttttt gtcctgcgga
     1621 ttgccgtcgc gggcgaacac gcggcagagt acgcgtttga cgttgccgtc caaaatggtt
     1681 tcgcggcggt tgaaggagaa ggcgcaaatg gcggcggcgg tgcttctgcc tacgccgcag
     1741 agggtttcca agtctttgcg ctccgacgga aacgtgccgc cgaattgcct gacgacttgt
     1801 tgcgcggctt tgtgcaggtt gcgcgcgcgg ctgtaatagc ccaagcccgc ccacaacgac
     1861 aacacttcgt cttgcggcgc ggcggcaagc gtctgaacgg tcgggaattt ttctaagaag
     1921 cgcggatagt agtccaacac ggtggcgact tgcgtttgct ggagcatgat ttcggaaagc
     1981 cagacgcaat aagggttttt gacctgccaa gggaggtggt ggcgaccgtg ttgtttttgc
     2041 cagcggatga gccgttcgga gaaggggatg ggtgtgttca ttaatatcaa tcggtggttt
     2101 tatttatatt taaaacggta tgttattgta taacattgtg aaaataattc ttattgactt
     2161 attttttgta ggggcatata actcatataa agaaacttta ttgcggtatt gaaattattt
     2221 atcaacaagc aaggagtatc agcatgaaag caatggttta tcacggcgca aacgacatcc
     2281 gttttgaaga aaaaccccgt ccgcagatta tcgatccgac cgatgcggtg gtgaaaatcg
     2341 tcaaaaccac gatttgcggt accgatttgg gtatttggaa aggcaaaaaa aacccgaagt
     2401 cgccgacggc cgtattctcg gtcatgaagg catcggtatt gtagaagaag tcggcgaggc
     2461 tgtaaaaaac atcaaagtcg gcgataaagt cattatttca tgcgtcagca aatgctgtac
     2521 ttgcgacaac tgcaaaatcc aactttattc acactgccgt aacggcggct ggattttggg
     2581 ctatatgatt gacggtacgc aggccgaata cgtccgtaac agcctcgtgc cgctgcccga
     2641 caacgtcaac gaagaagtcg ccctgttgtt gagcgacgcc ttgccgaccg cccaactgta
     2701 cagccctgcc gccatcatcg tttgcgatat ggacgaaaac cgtttgaaac tggcgaaaga
     2761 gttgggcgcg acccatacca tcagccccgc ttccggcgac gtctccaaac aagttttcgc
     2821 catcgtcggc gaagacggcg tagattgtgc catcgaagcg gtcggtatcc ctgcaacttg
     2881 gaatatgtgc caagacatcg tgaaacccga cggccatatc gccgtcgtcg gcgtacacgt
     2941 tcaatccgtt gatttcaaac tggaaaaact gtggattaaa aatcttgcca tcactaccgg
     3001 cctggtaaac gccaatacca ccgaaatgct gatgaaggca atttccagca gctccgtcga
     3061 ttacaccaaa atgttgaccc accgtttcaa attcagcgaa ttggaaaaag cctacgacgt
     3121 gttcaaacac gccgccgaaa atcaagcgat gaaagtggtt ttggaagcgg attaatcggc
     3181 gttgaatcag ttgtttttta agcggatgcc gtctgaatgc gtttcagacg gcattttttc
     3241 agctgcgtaa aacgggctgt ccggctgctg tcgaatgaac tgaacgttat ttttattatg
     3301 cggaaggcgg atgggcattt taggggcggt atgtctgatc agtgctgccg tgtatgccga
     3361 ctgtgccctc aatcggcaaa atgccgtctg aaacgggaaa gggttttaga cggcattttt
     3421 acgatgcggt tcaggctttg ggttttaacg ccgccgcttc tttggcgagg cgggtgatgg
     3481 tgtcccagtc tttgtttttc acagcttctt tcggggtcag ccaagagccg ccgacgcaca
     3541 agacgttggg cagtgccagg taatcgggtg cggtggcgag gctgatgccg ccggtcgggc
     3601 agaagcgcac gtcggcataa ggaccgtaaa gggctttgag catggctttg ccgccgacga
     3661 cttcggcggg gaaaagtttg agggtgtcga tgccgtgttc caaagccagt tggacttcgc
     3721 ccggagtggc aacgccggga atcaggggga tgccgctgtt gtggctggct ttggcgaggg
     3781 attcgtgcaa acccgggctg atggcgaaaa ccgcgcctgc gtcttcgacg gctttgagct
     3841 gttcggggtt ggtaaccgta cctgcgccga cgatggcgtt gggtacttct ttggcaatca
     3901 ggcggatggc gtcgaggccg acgggggtgc gcagggtaat ttcgagggtg gggatgccgc
     3961 cttcgacaag ggcgtgggac aaatcgacag cggtgctcaa gtcgtcaatc gccatcacag
     4021 gcacaactgc gcctgcgctc aggatttcgc ggggggtcag tttggacatt tcggttctcc
     4081 gggtggaatg gggtatttta ttaagatggg acaggtcgta ggtcggattc aagaaaccga
     4141 catcttggac ggctgtgtcg gattcgagaa tccgacctac gtctatctta aaacacttga
     4201 ataatttttc agacggcctt cggtctgaaa aggccgtctg aaacgcgcat caggcaaatt
     4261 cgccgccgaa actcatggca ccggtttccg cgctgctggt catgctgcgg aagttggcga
     4321 agagttcgcg gccgcagcct tgttggttcg cgcccaagtc gatgctttcg acttcgcggg
     4381 cattccattc ggtttcgtta atcaggacgt tgagttcgcc gctaacggag tcgaagcgga
     4441 tcaggtcgcc ggtacggatt ttggcgatgt tgccgcccat cagggcttcg ggtgtcatgt
     4501 ggatggaggc cggaactttg ccagatgcgc cggacatacg accgtcggtc agcagtgcca
     4561 ctttgaagcc gcggtcttgc aggatgccca aaggcggggt cagtttgtgc aattcgggca
     4621 taccgttggc acgcgggcct tggtagcgga cgacgcagac gaaatcgcgt tccaactcgc
     4681 cgcgttcaaa tgccgccaac acttcgcgtt ggtcgttgaa cacgatggca ggcgcttcaa
     4741 taatgcggca gccttcgcgc acggcggaca ctttaatcac gccgcgtccg atgttgcctt
     4801 tcatcaggcg cagaccgccg tcgggggaga acgggttgcc ggctttgcgc aggatgtcgt
     4861 cgttgccgct ggtttcgggg gcttcgcgcc attcgagttt gccgtcgata aggaaaggct
     4921 ctttggtgta gtggcgcata ccgtgtccga caacggtatc gacatcgtcg tgcaacaggc
     4981 ctgcgtccag caattcgcgg ataacgaaag gtagaccgcc ggcagcggta aagtggttta
     5041 cgtcggcttt gccgttgggg tacacacgga tcagcaatgg gatgatggaa gaaatttcgt
     5101 caaagtcgtc ccagttcaaa atcacgccgg cggcgcgtgc catggcaacg aggtgcatgg
     5161 tgtggttggt tgagccgccg gttgccatca ggccaatcaa ggcgttgata aaggattttt
     5221 cggtcaacat ttcgcccaaa ggtttaatcg tgccgttttt gatgccgcgc gcgaggtgtc
     5281 cggcggcgta gcgggtcagc gcttcacgca ggtcggtgta agggtggacg aaagcggcgg
     5341 cagggaggtg cacgcccatc atttccatca tcatttggtt ggagtttgcc gtgccgtaga
     5401 aagtgcaggt gcccgggctg tggtaagaac ccatttcgct tttcaaaagt tcgttgcgtc
     5461 cgaccttgcc ttcggcgaaa agctggcggg tgcgggcttt ttctttgttg ccgataccgc
     5521 tggacatcgg gcctgcgggg acgaagatac ccggaatatg accgaacgaa agcgcgccta
     5581 tcatcaaacc tggaacgatt ttgtcgcata cgcccataaa caggctgccg tcaaacattt
     5641 gatgcgacag cccgatggcg gtactcatgg caatcacgtc gcgggagaac agcgacaatt
     5701 ccatgccggc gtagccttgc gtgatgccgt cgcacatggc gggcgtgccg ccggcgactt
     5761 gggcggtcgc gccgtttttc tgcgcttcgt ctttgatttg gtcagggaag tctttaaacg
     5821 gctggtgtgc ggaaaccatg tcgttgtagg cggtgatgat gcctaagttg gggacggttt
     5881 cctgaagcat ttcgattttg atacttttag gcatggcagc atagccgtgt gccaagttgc
     5941 tgcagccgag ctggttgcgc tctaagcgtc ccatttgttt ggcactgcgg atcttcgcca
     6001 gatatttttc acgcgtcgga cggctgcgtt caataatgcg cccggtgatt tcggcgagtt
     6061 tggagtggat aggagtagtg ttcacggtcg gtctcctgtc ggaacggggg ggtattgggg
     6121 atttgtttgt gcgtttctga tttttgtatc gggtattcag acggt
//
LOCUS       DAXHNE010000116         6137 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.116, whole genome shotgun sequence.
ACCESSION   DAXHNE010000116 DAXHNE010000000
VERSION     DAXHNE010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6137
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.116"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            142..1539
                     /gene="aspA"
                     /locus_tag="ACFM4Z_001618"
     CDS             142..1539
                     /gene="aspA"
                     /locus_tag="ACFM4Z_001618"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFC6021628.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGKLGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEVLGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLAVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTTGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAINLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1611..2486)
                     /locus_tag="ACFM4Z_001619"
     CDS             complement(1611..2486)
                     /locus_tag="ACFM4Z_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HFC6021629.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWWLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGRLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGIFLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWGEVKVLFGI"
     gene            complement(2488..3210)
                     /locus_tag="ACFM4Z_001620"
     CDS             complement(2488..3210)
                     /locus_tag="ACFM4Z_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HFC6021630.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPKAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSCHTYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNGGRAVAPEKWI"
     gene            complement(3233..3712)
                     /gene="ybaK"
                     /locus_tag="ACFM4Z_001621"
     CDS             complement(3233..3712)
                     /gene="ybaK"
                     /locus_tag="ACFM4Z_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFC6021631.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPVQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3787..4149
                     /locus_tag="ACFM4Z_001622"
     CDS             3787..4149
                     /locus_tag="ACFM4Z_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC6021632.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKRDAATA"
     gene            4184..5047
                     /locus_tag="ACFM4Z_001623"
     CDS             4184..5047
                     /locus_tag="ACFM4Z_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HFC6021633.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINHLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     EASAEPLAAQEPSLAFTAECGSDGICSVVLPHRGILHIAPKNALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEEAGIVLDSVQDSENFTALTVIKFSAAQKTLTVPNGGIFKA
                     VHLFFKTENAE"
     gene            5140..6099
                     /gene="ttcA"
                     /locus_tag="ACFM4Z_001624"
     CDS             5140..6099
                     /gene="ttcA"
                     /locus_tag="ACFM4Z_001624"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFC6021634.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPDDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFVGLERGQSLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGS"
BASE COUNT         1559 a         1783 c         1484 g         1311 t
ORIGIN      
        1 cccgaaaaca acgtttcccg ttttcagacg gcatacgata tggacaattt catccggccg
       61 gtatagtgtt cgtcctgcgg cggcatcttt ccgatggcgc attttttcaa tattaattat
      121 ccgacttaca aaggtaaaaa catgactgtc cgtatcgaac acgatttatt gggcgaccgc
      181 gagattcccg ccgaagtgta ttggggcatc cacaccctgc gcgccattga aaactttaaa
      241 atctccacac aaaaaatttc tgacgtgccg cagtttgtcc gcagtatggt gatggtgaaa
      301 aaggcgacgg cacaggcaaa cggtaagttg ggcgcggtca agcccgaaat cgccgccgcc
      361 attgaaaagg cttgcgacga agttctgctg aacaaccgct gcctcgacca attcccgtcc
      421 gatgtgtatc agggcggtgc gggtacttcg gtcaatatga acaccaacga agtcatcgcc
      481 aacctcgcat tggaagtctt gggatacgaa aaaggccgct acgatatcgt caatccgatg
      541 gatcatgtca acgccagcca atccaccaac gatgcctacc ctaccggctt ccgccttgcc
      601 gtgtattaca gcatcggcga attgctcgac aaactggccg tcctgaaaaa cgcctttgcc
      661 gccaaagccg aagagtttaa agacgttttg aaaatgggcc gcacccagct tcaagatgcc
      721 gtgccaatga ctacaggaca agaattccaa tctttccaag tgttgttgga agaagaaatc
      781 ctcaacctcg accgcacgcg ccaactgctg ttggaagtca atttgggcgc aacggcaatc
      841 ggcacgggcg tgaacacgcc caaaggctac gccgaactgg tggtcaaaaa actctccgaa
      901 gtcagcggct tgccttgcaa actgaccgaa aacctgattg aggcgacctc cgactgcggc
      961 gcatatgtga tggtacacgg cgcattgaag cgcacggcgg tcaaactctc caaaatctgc
     1021 aacgacttgc gcctgctctc ttccggcccg cgtgccggct tgaaagaaat caacctgccc
     1081 gaattacagg ccggttcttc cattatgcct gccaaagtca atcccgtgat tcccgaagtg
     1141 gtcaaccaag tctgcttcaa agtcatcggc aacgatacga cgattacctt cgccgccgaa
     1201 gccgggcaac tgcaattaaa cgtgatggag ccggtcatcg cccaatgtat gtttgaaacc
     1261 atttccctct tgggcaatgc agcaatcaac ctgtccgaca aatgcgtcaa aggcattacg
     1321 gtcaaccgcg aaatctgcga acgttacgtt ttcaactcca tcggtttggt cacttatctg
     1381 aatccgtaca tcggccacca caacggcgac ttggtcggca aaatctgcgc ccagaccggc
     1441 aaaggcgtgc gcgaggttgt cttggaacgc gggctgttga gcgaagaaga actcaaccgc
     1501 atcctctctc ctgaaaacct gatgaacccc catctgtaac gttgcagaca aatgccgtct
     1561 gaagcaggga tgctgcttca gacggcattt tgttatatca atcagccgga ttatatgccg
     1621 aacagtacct tgacttcccc ccaataaaac aggcaggcga tagaacacag tgccaacacg
     1681 attcccgccg tattgattgt gttgattttt tccttaaaga acaatgcgcc cgaaagcgta
     1741 cccaaaacaa tcacgccgat attcataccg gcaaaaacca aggtcggatt atccttcatc
     1801 atttggtgcg cggtgatgta ggttacgata ttcataaaat tcaaaccgcc caagaatatg
     1861 ccgccaacca cactctcaac acgccatctg accgatttgg caaacaggca ggcaaacatc
     1921 agcacacccg ccagcacaaa tgcaaccagc aggttgcccg caaatgccgt tccgcttttg
     1981 gcaagctgtt tgaacaggat atcgataatg ccgtaacctg cccacacgcc cagcagcaat
     2041 gccgcctgcc gccacgcgct tcctgatttt ttgccaccgc tgtgtttcca aagcaggcag
     2101 aacagtgcgg caaatgcgag gcacagcccg attagtctgc cttcgctgag tttttcgcca
     2161 aacagcgtca aggcggcaac aatcggcaaa aacagcgaca aacgctgcgc cgcgtcggat
     2221 ttgacgatac cggcggattc tacggatttg cccataatga caaatacgga cggcagcagc
     2281 acgcccaatg cggcaaacag ccaccatgtc ggcaaaaatg cgccgatatt gccgacatcc
     2341 ggcttcaata ccagcagggt cagtatgacc gcgaccacat aattgacggc gaccgcctgc
     2401 gcgatgtcga tttttttctt ccttgccatt ttcagcaaaa tggaaaccga cacgctgaac
     2461 acgatgctga ttaacaggta agccatacta tatccatttc tcgggtgcaa ccgccctgcc
     2521 gccgttgaag cggataaagc cggcgatgat gcggtagaaa taccagacgt tcgttgcaat
     2581 aataatcagc acgccgatac ccaaaagggc agtcagcgca cccaaaatat aaagccaaaa
     2641 tgtgcgccaa aaggttttaa tcaggtattc ggtatgtgcg gcatagacaa tgctgtccaa
     2701 actgttccgt ttcatatagg cgagaatcac gccgactatg ggcatatagg cgaaaatcac
     2761 gccgactatg ggcatgaaca gcatgattgc acccaaaccg aacaggatgt aggcggcgag
     2821 gacatagttg cgcccgcttc cgccgccgga cggttcgtaa gtatggcacg aacgtgatgc
     2881 ctgccgttca aacgcctgtc tgaacgcctg ttcgcggcgc atctgttcgc ggtaaaactg
     2941 ctccctgcgc gcctgttcgc ggcggaatgc ctcttcctgc cgcccgcgtt cctcatgccg
     3001 tctgaaggac gcgtcgtatt gcatccgttt cgacaggtcg gaaagcgtat cgtaagcccg
     3061 ttggatttct ttgaactttt cttccgcctt cggattgccc ggattgcggt cgggatgata
     3121 tttcatcgcc agcttgcggt aggcgcgttt gatttcgtcc gctcccgcct gcggcgacac
     3181 gcccaatacg gcatacaaat ctttgtccat attgttcctt tatactttcc cgtcaaaccg
     3241 ccgcctgtat tgttttcggg ttcaaaatat ttaaatctcc gggacggatg ccgataatga
     3301 acccgcgttt tccgccgttg atatagatgg tttccaaatc catcaccgac tgttcgacgt
     3361 aaatatccaa ctttgtccgg atgccgaacg gcgttgtgcc gccgaccaga tagcccgtcc
     3421 acttgtttgc ctgtacgggc gtggcgggtt caatgtgttt cgcacccaaa tgccgcgcca
     3481 gattgcgggt tgaaatctgc ttgtcgccgt gcatcaagac aatcagcccc ttaccgtttt
     3541 catcttgcaa aacaatggtt ttaatgacca agtgttcgtc tttgccgaat agtcgggcaa
     3601 actgcgccgt gccgccgtgt tcctcatagg cataggtaaa aggttcaaat tcgatgccgt
     3661 tttcacgcaa aacgcgcacg gcaggtgtta tcggataagt gtgtttactc atttttcgtc
     3721 atgcaaaaca tggaaatcgg tcaagcgcaa tgttaccata tatccccttt atcgaaggaa
     3781 accgatatgg atatccgata tttcggcaca acgccccgct attccgaagc cgtcggcgca
     3841 aacggcttga ttttcctctc cggcatggtt cccgaaaacg gtgaaacggc tgccgaacag
     3901 actgccgacg tgcttgccca aatcgaccgc tggctggcgg aatgcggctc ggacaaagcg
     3961 cacgttttgg atgcggtcat ctatctgcgc gatatgggcg actatgcgga aatgaacggc
     4021 gtgtgggacg cttgggttgc cgccggcaga acgcccgccc gcgcctgcgt ggaagcacgg
     4081 ctcgcccgtc cggagtggcg cgtcgaaatc aaaataaccg ccgtgaagag agacgcggca
     4141 actgcataaa cagatttcct ttatatttca gagtacgcaa atgatgaaaa ccttactgcc
     4201 ctttttcggc gtggtgcgcg tcagcggcga agaccgccaa accttcctgc acggacagct
     4261 ttccaacgac atcaaccacc ttcagaccgg gcaggcgtgt tatgcgactt acaacacgcc
     4321 caaaggccgc gtcatcgcca atatgattgt cgtcaaccga ggcggcgatt tgctgctgat
     4381 tatggcgcaa gacctgcttg aagcaacggt caaacgtttg cggatgtttg tgttacgcgc
     4441 caaagccgtt tttgaaatcc ttgaagatta cgccgtcggc gcggaattag aagcatccgc
     4501 cgaaccgctt gccgcgcaag aacccagcct cgcctttacc gccgaatgcg gttcagacgg
     4561 catctgttcc gtcgtcctgc cacatcgggg cattttgcac atcgccccca aaaacgcctt
     4621 gccgccttat gatgccgccg ccgaaaacgc gtggcggctg cacgaaatcc gtagcggtta
     4681 tccgtggata tgcgcggcta ccaaagaaac cgccgtcgcc caaatgctca accagcacat
     4741 catcggcggc gtacacttca aaaaaggctg ctatccggga caggaaatca ttgcccgtgc
     4801 ccagtatcgg gggcaggtca aacgcggctt ggcggtattg tcgggaaatt cggccgtcga
     4861 agcaggaacc ctgctgacgg cggacggcga agaggccggc atcgtactcg acagcgttca
     4921 agattcggaa aactttaccg cccttaccgt gattaaattc tccgccgcac aaaaaacact
     4981 gaccgtccca aacggcggta tcttcaaagc ggttcatctc ttctttaaaa ccgaaaatgc
     5041 agaataagcc gccgatgccg tctgaagccc ttcagacggc attgccgccc cgccaatcgg
     5101 ttacaatgcc gcctttctaa ttttcaaacc caaaccgcca tgtccaaaaa aaccaaacaa
     5161 gaactcgaaa acaacaaact cagcaaacgc ctgcgccacg ccgtcggcga cgcgattaac
     5221 gatttcaata tgatcgagcc ggacgacaaa atcatggtct gcctctccgg cggcaaagac
     5281 agctatgccc tgttagacat cctgcgccag ctccaagcca gcgcgccgat tgatttccaa
     5341 ctggttgccg tcaatctcga ccaaaagcag ccgggcttcc ccgaagaagt attgccgacc
     5401 tatctcgaaa gcatcggcgt tccctacaaa atcgttgaag aagacaccta ctccaccgtc
     5461 aaacgcgtat tagacgaagg caaaacgact tgttcgctgt gcagccgcct gcgccgcggc
     5521 atcctctacc gcactgcaaa agaattgggc tgcaccaaaa tcgccttggg acaccaccgc
     5581 gacgacatcc tcgccacctt gtttttaaat atgttttacg gcggcaaact caaagccatg
     5641 ccgcccaagc tggtgagcga caacggcgaa cacatcgtca tccgcccgct ggcgtatgtg
     5701 aaagaaaaag acttaatcaa atacgccgaa ttaaaacaat tccccatcat cccgtgcaac
     5761 ctctgcggct cgcaacccaa cctgcaacgc caagtcatcg gcgatatgct gcgcgattgg
     5821 gacaaacgct tccccggccg catcgaatcg atgttctccg ccctgcaaaa cgtcgtccct
     5881 tcccaccttg ccgataccga actcttcgac tttgtcggat tggaacgcgg tcaaagcctg
     5941 aaacacggcg gcgatttggc gtttgacagc gaaaaaatgc ccgaacgttt ttcagacggc
     6001 agcgaagaag acgagagcga aatcaaaatc gagccgcaaa aagccgaacg caaagtcatc
     6061 aatattctgg cgaacaagcc gaaaacctgc ggctcataga cctccgccgt caaaaatgcc
     6121 gtctgaagcc cgtttgg
//
LOCUS       DAXHNE010000117         6053 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.117, whole genome shotgun sequence.
ACCESSION   DAXHNE010000117 DAXHNE010000000
VERSION     DAXHNE010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.117"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(371..955)
                     /gene="rnhB"
                     /locus_tag="ACFM4Z_001625"
     CDS             complement(371..955)
                     /gene="rnhB"
                     /locus_tag="ACFM4Z_001625"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFC6021635.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAVEWHVAAASPEEIASLNILHATMLAMKRAVDGLAVRPEKIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALEKYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(1024..2919)
                     /gene="mnmG"
                     /locus_tag="ACFM4Z_001626"
     CDS             complement(1024..2919)
                     /gene="mnmG"
                     /locus_tag="ACFM4Z_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFC6021636.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAATRMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGERISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLEHYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIAGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVREQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVSEAQWRMFNEKREAVEREIQRLKTTWYTPQKLAEGEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            complement(3244..4671)
                     /locus_tag="ACFM4Z_001627"
     CDS             complement(3244..4671)
                     /locus_tag="ACFM4Z_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6021637.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            4831..5823
                     /gene="pdxA"
                     /locus_tag="ACFM4Z_001628"
     CDS             4831..5823
                     /gene="pdxA"
                     /locus_tag="ACFM4Z_001628"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFC6021638.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKNLLRA
                     RAEALGKSVVLRDFDPESGGASEEGVLEVLHIPAAEAVEAGKLNPANAAYVLQLLDTA
                     LAGISDGIFDGIVTAPLNKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLR
                     VALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGH
                     LGHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKY
                     HSFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
BASE COUNT         1249 a         1582 c         1783 g         1439 t
ORIGIN      
        1 aaggcgggaa tctaggtctg tcggtacgga aacttatcgg gtaaaacggt tttgccagcc
       61 ctgagactcc ggattcctgt tttcgtagga atccggtttt ttgagcttca gtcatttttg
      121 ataaattctt gcagctttga gtttctagat tcccgctttc gcgggaatga cggtttggaa
      181 gttacctgaa attcaaaaaa aaaaaacgga aaccggacgg attggattcc cgcctgcgcg
      241 ggaatgacgg attttaggtt tttttttgat tttctatttt tcgcgggaat gacggtttgg
      301 gttctttctc tttggagttg cgatgccgga aatgccgtct gaaggcttca gacggcattt
      361 ttgtgccggt ttaaaacaag gcctgctgcg cgagcaggtt tctgacgggg gcgaagtcgc
      421 ggcggtgttc gggcagcacg ccgtattttt cgagggcttc caaatgctgc ttcgtgccgt
      481 aacctttgtg tttgtcgaaa ccgtattggg gatggcgttg cgccagtgcg tacatttccg
      541 catcgcgtgc ggtctttgcc aaaacggatg cggcggagat ttcgatgatt ttgctgtcgc
      601 ctttgacgac ggcttcggca gggatgttca aatgttcagg aatgcggttg ccgtcgatga
      661 atattttttc gggacgcaca gccaagccgt caacggcgcg tttcatcgcg agcatggtgg
      721 cgtgcaggat gttgaggctg gcgatttctt cgggcgaggc ggcggcaacg tgccactcaa
      781 ccgcctgatt ttttatcatt tcggcaagcg cgtcgcgttt tttctcgctg agttttttgg
      841 agtcggtcag tccgggcagg tcgaatgttt ccggaaggat gacggcggcg gcaaacacgc
      901 tgccgactaa aggtccgcgt cctgcctcgt ccacgccggc ggtcagtatg tgcatgatgt
      961 ttcctgtcgg gatggtggga atgccgtctg aaaagggttt cagacggcat cgcgccgatg
     1021 tgtttatttc gcgtctttaa acccgcgctt caaatgcacc atcagcaatg ccactgccgc
     1081 aggggttacg ccggaaatgc ggctggcttg tccgacggtt tcgggtttgt gctggttgag
     1141 cttttgctgc acttctgccg acaagccttt gactttgccg taatcgatgc cgtcgggcag
     1201 ttttaaggtt tcgatgtcgc ggcggctgtc gatttcttcg ttttggcggt cgatatagcc
     1261 ttggtatttg acttggattt cgacttgttc gatgacttcg gcggagaggt tttcagacgg
     1321 catcgcgcct tcgagcgtca tcagcgcggc gtagtcgagg tttgggcggc gcaggaggtc
     1381 gtgcaggttg gcttcgcggc tgagtttttg tccgaacaca cggatttgtt cgccttcggc
     1441 gagtttttgc ggcgtgtacc acgttgtttt caaacgttgg atttcgcgtt cgacggcttc
     1501 gcgtttttcg ttgaacatgc gccattgcgc ttcggacacc aagccgattt tgtagccgtc
     1561 ttcggtcagg cgcatgtcgg cgttgtcttc cctgagttgc aggcggtatt cggcacggct
     1621 ggtgaacatt cggtagggtt cgttcacgcc tttggtgatg aggtcgtcca ccaatacgcc
     1681 gagataggct tgttcgcggc gcagcaggag cgggtcttgt tcgcggacgt attgcacggc
     1741 gttcgcgcct gccagcagtc cttgcgcggc ggcttcttca taacctgtcg taccgttgat
     1801 ttgcccggcg aaaaacaatc cggcaatggt tttggtttcg aggcttgctt tgaggttgcg
     1861 cggatcgaag tagtcgtatt cgatggcgta gccggggcgc aggatatggg cgttttccaa
     1921 acctttcata ctgcggacga gcgcgatttg gatgtcgaac ggcaggctgg tggagatacc
     1981 gttggggtag tattcgtgcg tggtcaggcc ttcgggttcg aggaaaatct ggtggctgtc
     2041 tttgtcggcg aagcggttga ttttgtcttc gatagacgga caataacgcg ggcccacgcc
     2101 ttcgattttg ccggtaaaca tcgggctgcg gtcgaagcct gagcggatga tgtcgtgggt
     2161 ttgcgtgttg gtatgcgtaa tccagcagga cacttggcgc gggtgcattt cggcgttgcc
     2221 gcgtacggac atgacgggaa cgggcgtgtc gccgggctgt tcggtcagtt gggagaagtc
     2281 aatcgtgcgt ccgtcgatgc gcggcggcgt gccggttttc agacggcctt gcggcagctt
     2341 caattcgcgc aaacgtccgc ccagcgattt ggcggcgggg tcgccggcgc gtccgccttc
     2401 gtagtgttcc aaaccgatgt ggattttgcc ggacaaaaac gtgcctgcgg tcaacacgac
     2461 ggcgcgtgct ttaaactcca cgcccatcgc ggtaattacg ccgctgatgc gttcgccgtc
     2521 gagcgttacg tcttcgacgg cttgttggaa aaggtcgagg ttttcttggt tttccaacat
     2581 ttcgcggatg gcggctttat acaaaatgcg gtcggcctgt gcgcgcgtgg ctcgcactgc
     2641 cgcgcctttg ctggcgttca ggcggcggaa ttggatacct gatttgtcgg ttgccaacgc
     2701 cattgcgcca ccgagcgcgt cgagttcgcg taccaaatgc cctttgccga tgccgccgat
     2761 ggaggggttg cacgacattt gtccgagcgt ttcaatattg tgtgtgagca aaagcgtctg
     2821 cgcgcccatg cgtgtggcgg caagcgcggc ttccgtgccg gcgtgtccgc cgccgacgac
     2881 gataacgtcg taggttttgg ggtaaatcat gtgggtcatg tgtgtattgc ctgtcggtgt
     2941 ttcagacggc atttatagtg gattaacaaa aaccagtaca gcgttgcctc gccttagctc
     3001 aaagagaacg attctctaag gtgctgaagt accaagtgaa tcggtttcgt actgcttgta
     3061 ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaaa ccactatatt caatatgccg
     3121 tctgaaaaac gaaatggatt caaaagtaaa gggttgggat tgtacgcttg ttcgccctgt
     3181 ttttacagtg tgcggaaagg gaaaagccgc ttcgcgggga agcggctccg gtaagggcgg
     3241 gatttaccaa acgtcggatt tgatacggcg tttcaggccc ggatgttcgg aaagtttgaa
     3301 ctcggggtct ttgcccattt tcagcttggc ggtgtaatcg cgcagcagca taaacgccaa
     3361 gggcgagagc agcaggatgg cgacaaggtt gatccacgcc ataatgccca tcgccatatc
     3421 cgccatatcc cagaccaaag gcacattggc aaccgcgccg aaatagaccc acgccaaaac
     3481 cagcatacgg aaaacggcgg taatcagcca atggcttttg atgaattgga cgttggactc
     3541 ggcataggca tagttgccga taacggtgga aaaggcaaac ataaacagga tgacggcgag
     3601 gaagcccgcg ccccattgcc ccacttggct gacaatcgcc gcctgcgtca gcgccgcacc
     3661 gctcaaatcg ccgtaaggct gttggtaaat caagatgatg aaggcggtgc aagaacaaac
     3721 gatgatggta tcgacaaaca cgcccagcat ttgaatcata ccttgcgaaa cagggtgttt
     3781 cacttcggcg gcggcggcgg cgttcggcgc ggaacccata cccgcctcgt tggaatacag
     3841 gccgcgtttg atgcccatca tcatcgtttg cgaaatcaga ccgccgagta agccgcctgc
     3901 tgccgcgtcg aatttgaacg cgcccgaaaa aatctgaccg aacacgtccg gaatcatcgg
     3961 aatattggtc aaaatgatga aaagcgcgat aaagaggtac aaaaccgcca tcagggggac
     4021 gacgatttcc gccgctttag atatgcgcct gatgccgccg aagataatcg gcgcggttaa
     4081 aatcaccagg gcgacgccga cataatgagg ctcccaaccc catgccgctt tgacggtatc
     4141 ggcgatggta ttggtctgaa ccgcttcaaa cacaaagccg aaacagaaaa tcaggctcag
     4201 ggcgaacaac acgcccagcc atttctgccc cagcccttga gtgatgtagt aggcagggcc
     4261 gccccggaaa tggtggttgt cgtagtcgcg gactttaaag agctgcgcca gcgaagattc
     4321 gacaaacgcc gaactcatac cgattaaggc ggttacccac atccaaaaca ccgcgcccgg
     4381 tccgccgact ttgatggcga tggccacgcc cgcgatattg cccacgccca cgcggctggc
     4441 aaggccggtt acaaatgcct gaaacggcgt gatgccgtga gggtcgtccc cctgtttgcg
     4501 gccgccgagc atttctttga tgctgcgccc gaacaggcgg aattggacaa agcccgtggt
     4561 tacggtgaag aaaagccccg tacccaaaag catataaacc aagtatgacc acatcggatc
     4621 gttgatggcg ccgacccagc cgtgcagcca ttcggtaaag ttctcgttca tatcgcttcc
     4681 ttaaagttga aactcgcaca tattggcggt atgcaagcag ggtttaaatt ttgtaaacgc
     4741 ccattctagc agattgtcaa caaaatcaga aaaatttaca tcgccgcgcg gctgcggcgt
     4801 tagaatcgca ttttgtttgg agcaaacacg atgaaacagc ctgtttttgc cgttacttcc
     4861 ggcgagcctg ccggcatcgg ccccgatatt tgtttggact tggcatttgc acgcctgccc
     4921 tgccgctgcg cggtattggg cgacaaaaac ctgttgcgcg cgcgcgccga agccttgggc
     4981 aaaagcgtcg tcctgcgcga cttcgatccg gaatcaggcg gcgcgtccga agaaggcgtg
     5041 ctggaagtgc tgcacatccc tgccgccgaa gcggttgagg cgggcaaact caatcccgcc
     5101 aacgccgcct atgtgctgca acttctagac accgcgctcg caggcatttc agacggcatt
     5161 ttcgacggca tcgttaccgc gccgctgaac aaaggcatca tcaacgacgc gcgcgcaagc
     5221 acaggttttt tcagcggaca caccgaatat ctggcggaaa aaagcggcac ggggcaggtt
     5281 gtgatgatgc ttgccggcaa aggcctgcgc gtcgccctcg taacgaccca cctgccgctg
     5341 aaagacgttg ccgccgccat cacgcaaccg ctgattgaaa gcgtcgcacg cattttgcat
     5401 cacgacttaa aacacaaatt cggcatcaaa aatcccaaaa tcctagtcgc cggactcaac
     5461 ccccacgccg gcgaaggcgg acacctcgga cacgaagaaa ccgacaccat tatccccgcg
     5521 ctcgaaaacc tgcgccgcga agggataaac ctcgccggcc cgtatccggc ggataccttg
     5581 ttccaaccgt ttatgctcga aggtgcggat gccgtattgg cgatgtacca cgaccaaggg
     5641 ctgcccgtgt tgaaatacca cagcttcgga cagggcgtga acatcacgct cggcctgccc
     5701 tttatccgca cctccgtcga tcacggcacc gcgcttgatt tggcggcaac cggcagggcg
     5761 gattccggca gcctgataac tgccgtggag accgccgtcg agatggcgcg cggcagcctt
     5821 taaagatgat aaaagacccg tcatttccgc gcaggcggga atccggtctg ttcggtttca
     5881 gttgtttttg ggtttcgggt aatttccaaa tcgtcattcc cgcgcaggcg ggaatccaga
     5941 ccattggaca gcggcaatat tcaaagatta tccgaaagtt tgaggttcta gattcccgtt
     6001 ttcacgggaa tgacgaaagg tggcgggaat ccggtctgtt cggtttcggt ttt
//
LOCUS       DAXHNE010000118         6036 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.118, whole genome shotgun sequence.
ACCESSION   DAXHNE010000118 DAXHNE010000000
VERSION     DAXHNE010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.118"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(72..1289)
                     /gene="nqrF"
                     /locus_tag="ACFM4Z_001629"
     CDS             complement(72..1289)
                     /gene="nqrF"
                     /locus_tag="ACFM4Z_001629"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HFC6021639.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNNEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLVAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            complement(1303..1896)
                     /gene="nqrE"
                     /locus_tag="ACFM4Z_001630"
     CDS             complement(1303..1896)
                     /gene="nqrE"
                     /locus_tag="ACFM4Z_001630"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HFC6021640.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            complement(1900..2526)
                     /locus_tag="ACFM4Z_001631"
     CDS             complement(1900..2526)
                     /locus_tag="ACFM4Z_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HFC6021641.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            complement(2526..3302)
                     /locus_tag="ACFM4Z_001632"
     CDS             complement(2526..3302)
                     /locus_tag="ACFM4Z_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HFC6021642.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVADAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            complement(3295..4527)
                     /locus_tag="ACFM4Z_001633"
     CDS             complement(3295..4527)
                     /locus_tag="ACFM4Z_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HFC6021643.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYTLAD
                     ALGINMSSEAGVSGKMLFGAIYFLPIYATVFVVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLSLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            complement(4530..5873)
                     /locus_tag="ACFM4Z_001634"
     CDS             complement(4530..5873)
                     /locus_tag="ACFM4Z_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HFC6021644.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPVITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPVSGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPTVIIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTERVVALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
BASE COUNT         1444 a         1567 c         1618 g         1407 t
ORIGIN      
        1 gtgttcggac ggtatcggat aaaaaaagga agaaaccgag gtttcttcct tttgtgtttg
       61 aagccgaata tttaaccgcc gaaatcgtcc aagaggatgt tttcgtcttc cacgcccaag
      121 tctttgagca ttttgatgac ggactggttc atgattggag gaccacacat ataaaattcg
      181 cagtcttccg gtgcttcgtg atttttcagg tggttttcgt aaaccacgtt gtgaatgaag
      241 cctgtgtagc cgtcccagtt gtcttcgggc aatgggtcgg acagggcgac gtgccacgta
      301 aagttcggga actctgccac gagttggtcg aagtcttcga catagaacat ctcgcgtttg
      361 gaacgcgcac cataccagaa ggtgattttg cgtttggagt tcaaacgttt caactggtcg
      421 aaaatgtggg aacgcatcgg cgccataccc gcaccgccgc cgataaatac catttcggca
      481 tcggtgtctt tggcgaagaa ttcgccgaac gggccggaaa tcgtaacttt gtcgccgggt
      541 ttgagcgacc agatgtaaga agacatttgt cccggaggcg catcgggtac gcgcggaggc
      601 ggcgtggcga tacgcacgtt cagcataatg atgccttttt cttctggata tgaagccata
      661 gagtaggcgc gcaaaatcgg ctcgtccact ttggaaacgt attgccacaa attgtatttg
      721 tcccagtctt cgtgatattc cttaggaatg tcgaagtctt tgtaggcaac agtgtgagga
      781 ggagcttcaa tttgaatgta gccgccggcg cggaagggga cttcttcgcc ttcgggaatg
      841 gcaagcttga gttctttaat gaacgtggct ttgttatcgt tggagatgac ggtgcattcc
      901 cattttttca cgccgaacac ttcttcgggg acttcgatgt ccatgtcggt tttgacgttg
      961 acttggcacg acagacggca gccttcgcgt gcttcgcgtt tgctgatgtg ggacagctcg
     1021 gtcggcagga tgtcgccgcc gccgcttttt acgacgacgc ggcattgtcc gcacgaaccg
     1081 cccccgccgc aggcggaggg gataaagatg ccttcgttgg caagcgcgcc caagagtttg
     1141 ccgccggcgg gcatcgtcag ctctttttcg ttattgactt tgatggtgat gtcgccttcg
     1201 ctgaccagtt tggatttggc aaacagaatc atcagtgcca aaaccaaaac gatgacggta
     1261 aacatcacga tacctaaaat aatctccata ccgatccctt tcttataact ggatgccgga
     1321 gaacgacata aacgccatcg ccatcaggcc ggcggcgata aaggtaatgc ccaagccttt
     1381 gaggcctttg ggagcgtccg aatatttcat tttttcggta atgcccgcca aagcgacaat
     1441 cgccaacatc cagcccaagc ccgcgccgaa accgtataca acggactcgc cgaagttgta
     1501 ttcgcgttgc gccataaacg atacggcgcc gaaaatcgcg cagttcacgg taatcagcgg
     1561 caggtagatg cccaacgcgt tatagagggc ggggacgaat ttatccaaga acatttccaa
     1621 aatctgcacc aaagcggcaa tcacgccgat gaaggtgatg aatttcaaaa aggtcaaatc
     1681 cacgccttcg acaatcgcgc cgtctttgag cagcgagtaa acgagttggt tgacagggac
     1741 ggacagcccg agtacgaaaa ttaccgccac acccaaaccg aatgcggtgg atactttttt
     1801 ggataccgcc aaaaacgtgc acatacccaa aaagaaggat agtgccatat tttcaatgaa
     1861 gacggatttg atgaagaggc tcaaatagtg ttccatagct tattcctccg cctgttcggg
     1921 tttccaggta cgcagtcccc aaatcaaaaa gccgatgatg aagaacgcgc tgggggcgag
     1981 caggaacaag ccgttggtct gataccagcc gccgtcctgc acggtttgga aaacggtgta
     2041 gcccaagagt ttgcccgagc cgatcagttc gcggacggtg gcgacgacaa gcagcatcat
     2101 cccgtagccc gcgccgttgc cgatgccgtc gatcaggctt tccagcggcg gctctttcat
     2161 cgcaaatgct tcggcgcggc ccatcacgat acagttggta ataatcagac cgacgaatac
     2221 ggaaagctgt ttggacaatt cgtaggcaaa tgcctgcaag agttggtcga ccagcgtaac
     2281 cagcgacgcg ataatcgcca tttgcacgat gatgcggatg ctgttgggga tgtagttgcg
     2341 taccagcgaa atgaagaagc tggaaaaacc ggttaccaaa gctacggaaa tacccatcac
     2401 gatggccgtc tgaagtttgg tggtaaccgc caaagccgaa caaataccca aaacctgcaa
     2461 ggcaatcggg ttgttgtcga taaagggtga aaacatcaaa tgtttcaagc gtttcatatc
     2521 agccattatt gtgctcctgc tgatttcaat ttgttcaggt aggggatata gccgttttcg
     2581 ccgaaccagt aggcgaacga accttgcacg cctttggaag tcagcgacgc gccggagagg
     2641 gcatctacgc cgtgttcttt gtccgaaccc gcgcctttgc cgacgtgcag ggcgagtttg
     2701 ccttgtccgt caaacagttt tttgccgacg aatttttgct gccacaaagg gttgccgatt
     2761 tcgccgccca agcccggggt ttcgccttgc tcgtaatagg taatgccgtt gatggtgttg
     2821 ccgtcgggtt ggatggcgac aaaaccgtac atcaccgacc acaaaccgtt gccgtgcata
     2881 ggcaggatga tttgcccgat tttgccgtct tcacctttta ccaaataaac ctcagtgtat
     2941 ttggcacggc ttttgatgcc tgccaaatcg tcttcgggtt tgatgcggat gctttgggca
     3001 gggtctttgc ctgcgatgcg cgcgctgaaa tctttaggcg catcggcgac gtattcgccg
     3061 gtcgccaaat cgacaacacg ttgctcgata cgctcggcaa aggttttacc gatgtcggtg
     3121 tccttatcca tcaaaccggc tacgctcaag atatagcctt gtttgtcttg gagtttttgt
     3181 ttctcttgga tgggtttcaa gccaacgacc gcaccggcaa cgatgaccga gcaaatcagg
     3241 ctgaccgcca acacgacaat cagcgtgccg ctgaagctgt ctttatcgaa tttcttagcc
     3301 attgctgcgc gcctttctgc gtttgatgtt cgcttgtgcg acgaaatagt cgaaaatcgg
     3361 ggcaaacagg ttggcaaaca gaatcgccaa catcatgcct tcggggtaag ccggattgac
     3421 cacgcggatc aatacgcaca tcacaccgat tagcgcgccg taccaccatt tgccgacatt
     3481 ggtaaaggaa gcggaaacag ggtcggttgc cataaacagc ataccgatgg cgaagccgcc
     3541 gacgaccaaa tgccagtacc aaggcatagc aaacatggcg ttggtgtccg aaccgatgaa
     3601 gttgaacagc gaagacatgg caatcatacc gatcatcacg ccggcaataa tgcgccaaga
     3661 agcgatgcgg gcaaacacga taaacgcgcc gccgattaag agtgccaaag tggagacttc
     3721 gccgatggag ccgggcagtt tgccgataaa cgcatccatc caagtgatgg tttgaccggt
     3781 tacggcgttt ttcaggccgt ctgcaccgtg tgccgcccat tgcgccagcg cggttgcgcc
     3841 ggaatagccg tcaaccgccg tccaaaccgc atcgccgctc aagttggcag ggtaggcgaa
     3901 gaacaggaag gcacggcctg ccagcgcagg gttcatgaag tttttacctg taccgccgaa
     3961 tacttctttc gcaaccacaa cgccgaaaga aatacccaaa gctgcctgcc acagcgacag
     4021 cgtgggcgga acgattaagg caaacagaat cgaggtaaca aagaaacctt cgttgatttc
     4081 atgtttgcgc acggtggcga acaaaacttc ccagaaaccg cccacgacaa atacggtcgc
     4141 gtaaatcggc agaaagtaaa tcgcgccgaa cagcattttg cccgacacac ccgcttcaga
     4201 cgacatattg atgcccaaag cgtcggcaag ggtgtaatgc cagtcgttgg cgatgctttg
     4261 ttgcagcaaa tcgggcgtta aggcaccgaa tgcctgcgcg ccgacgttgt acataccgta
     4321 gaacatggca gggaacaaag ccagccacac caaaatcatc atgcgcttgg agtcgagcgc
     4381 gtcgcggacg tgcgccgctt tgcgcgttac cgcgccggat gtgtagaaaa ttgtcgccgc
     4441 cgcttcgtag agggcatacc atttttcatg tttgccgccc ggcaggaagt gcggttcgat
     4501 tttttccaga aaatgtttca agcccataat cagccttcct tctcaatggt ttccagcacc
     4561 ttacgcaaca gcgggccgta ttcgtatttg cccgggcaga cgaagctgca caaagcgagg
     4621 tcttcttcgt ccaattccaa gcaacccaat gcctgcgcgc tgtcggtatc gccgacgatt
     4681 aaatcgcgca aaagcagggt gggcaggata tccaagggca tcacgcgctc gtaagtgccg
     4741 atgggaacca tagcgcggtc gccgccgttg acggctgtcg tgaacttgaa gagtttgttt
     4801 ttcaggaaat ggccgagggt cgtccgcgtg atggagtatt tgtccggctg cggcgcaacc
     4861 cagccgaaca gctctttgct gcggccttct tcgataacgg aaatctgatt gtggtagcgt
     4921 cccaaataat catgcgcgcc ttgtgcaatc gcgccgttca ataccgaacc ggaaatcacg
     4981 cggttgtctg cgtcaaccaa ttcgcccgca gtaatttgcg atactttcgc acccaaaacg
     5041 gtacgcaaga ggcgtggttt gttgacttga gaaccaccca aggcaaccac gcgctcggtg
     5101 ttcagacggc ctgttgcaaa caaacgtccg atggcaatta catcttgata attgatggtc
     5161 caaacggttt tgttcgcacc gacaggctca atgaaatgaa tgtgcgtgcc actcaaaccg
     5221 gcaggatgcg ggccgccaaa ttcatgagtt tcgatgttgg cggcgttttc agacggcacg
     5281 tctgcgccgg ctgccttaca aacatggatt ttgcgttcgg tcaaacggct caacaccaac
     5341 aggccgcgtc tgaaatcctc ggcggcttct ttgataatga ccgtagggtc ggcagccagc
     5401 ggattggtgt ccatcgcatt gacgaagatg gcgaacggct cggcatcgac ggcagggatt
     5461 ttgctgaacg gacgggtacg cagcgcagtc cacaaaccgg attggatcag attgcggcgc
     5521 acttcttcgc cgcttaagtt tgccaacgct tcgggcgcgt agcgttcgaa ctcgatttcg
     5581 tcgttgcctt caacggcaat cacgaccgac tgaagtacgc gcttttcgcc gcgatggatg
     5641 gcggcgattt tgcctgaaac cggcgcggta aacaccacgc ccggattctt tttgtcttca
     5701 aacagcactt ggcctttttt gacggcatcg ccttccttga ctttcatcga ggggcgcata
     5761 ccggcatatt cttcgccaag caacgcgact tcggtaatga cgggcccgtc ataaatgact
     5821 tgctccggtc tgcccgcgat gggcaggttt agaccttttt tgattttaat catatatttg
     5881 cattacttgt gatggttaag gtaaaaacgg cgtgttttga taccgtgtcg cgtggcatca
     5941 aaagcattga ataaattaat gtagcaaagt gttagattct atcaggaatt gtacctgttt
     6001 gtcagatttg ctgctttttt ccttgcggaa gccgtt
//
LOCUS       DAXHNE010000119         6024 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.119, whole genome shotgun sequence.
ACCESSION   DAXHNE010000119 DAXHNE010000000
VERSION     DAXHNE010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.119"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="ACFM4Z_001635"
     CDS             complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="ACFM4Z_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HFC6021645.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAALNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPEDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYAAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="ACFM4Z_001636"
     CDS             complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="ACFM4Z_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HFC6021646.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="ACFM4Z_001637"
     CDS             complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="ACFM4Z_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HFC6021647.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAEQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPVVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFVVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIASITGAKKVTPKEWAPSENQA
                     AAPQSGVQTASEAKTASEAE"
BASE COUNT         1353 a         1709 c         1622 g         1340 t
ORIGIN      
        1 aaagaaatgc cgtctgaaac cttatcgttt cagacggcat tttattgccc gaccggttat
       61 ttgtcggttt gggtatcccg tttcaatccg ccgccgagtg ccttgtacaa atcggcaagg
      121 ttttcggcgc gggtcagttg tgccgacaaa gccgcaccct ccgccgcata gctgctgcgt
      181 tccgcatcga gcaaatcgag cgcgccggat acgccgtgct tgtaacgcag gccgaccaag
      241 cgcaacgctt ctttagaggc gcggctttgt ttgcttaaag cgtcataggc tttatccagc
      301 tgctcgcgcg ccgccaatgc gtttgccacg tcttgaaatg cggattggac ggcggattca
      361 taggcaacga tttgtgcctg ttggcgcagc ttggctacat caaggttcgc cttgttcgta
      421 ccccaggtaa aaatcggcag ggtaatagac ggcgcgaacg accaaacgcc cgtgccgctt
      481 ttgaacaacc cacccaattc ggcagaaccc gtaccgacgg ttccggtcag gcggatggat
      541 gggaaaaagg cggcgcgtgc cgcaccgata ttggcgtttg cctgtttgag cgcgtgttcg
      601 gcagcacgga tatcgggacg gtcgagcaat acttcggaac tcaaaccggc cggcagtttt
      661 tcaacaaaaa actgcttgtc cagcggcaaa ccggcaggca ggtcttcggg tatcggttgg
      721 ttaatcaagg ttgccaaggc attgcgcgcc tgttcgcggc tgcgcgcggc atgggcataa
      781 tcggctttgg cagattcgat cagggcttcc tgctgacgta gggcgacggc ggaaatcacg
      841 cctgccttgt aacgtaattc ggacagcttg taggtttcct cgcgcgtttt caaaacacgt
      901 tgcgccaaag acatcgcttc ttcggcgtaa cgttcgttga aataggcttt ggcaacggtg
      961 gcaatcaggc tcaaatgtgc cgcatcgcgg ttggcggtgc tggcgaaata gccttgcagt
     1021 gccgcctcgc tgctgctgcg tacacgcccg aacagatcga gttcgtaaga tgccgcaccc
     1081 agtccgactt tgtagctgct gcttacattg ccgccgctca agctgccttg gcgcgagtcg
     1141 ttcgcattgg cggcaagcgt gggcaggagg ttgttgcgct caatcatgta ttgtttgcgg
     1201 tagatttcgc tgttcaatgc ggcggtacgc aaactggtat tgcgctcgag tgcgatgtcg
     1261 atcagctttt gcaggcgcgg gtcggcaaaa tagtcatgcc aacctaaatc gaccgcacgg
     1321 atgccgctgt cggcggtatc gtttttgaac gtttcggcaa cttcgacttt gggctgctcg
     1381 tattggggaa tcatggtgca ggcggataat gcgaaggctg ctgcaacaga agtcaaggtg
     1441 gttttcaatg tagtatccat aaaaaagtcc tgatgccgtc tgaaaacccg tgggcgttca
     1501 gacggcatgg ttgcttaatg ttggctgtcg tccgaaccgg tgatgcccgc ttcggcggcg
     1561 tgttttactg ccatttcgtg ttcgtgcgcg gtttctttga agaatttgcg caccaccaca
     1621 tagaaaagcg gaacaaggaa cacggacaag agcgtgccga tgagcatccc ccagaatacg
     1681 gtcgtgccga tggcgcgctg gctggcagaa cttgcaccac cggcaatata caggggaacc
     1741 acgcccaaaa taaaggcgaa cgaggtcatg ataatcggac ggaaacgcag gcgggcggct
     1801 tccaaagcgg cttcgaccgc gcttttccct tgcgcttgaa ggtctttggc aaactcgata
     1861 atcaaaatcg cgtttttcgc actcaaaccc atcacggtaa cgaaaccgac ttgaaagtag
     1921 atgtcgttgg cgaacgaggg aacgctgccc aacagtcctt caaacaggtt gcgcccggtt
     1981 acgcccgcag ccgcaccgat caaacccaac ggaatcacaa ggatgaccgc cagcggaatc
     2041 gaccagcttt cataaagcgc ggcaagtacc aaaaatacgg ctgcaaccgc caaaccgtac
     2101 aaaatcaggg tttgcgagcc gccttttgcc tcttcgcgcg actgtccgcc ccattcgaag
     2161 ctgtaaccgc cgcccaattc gtcaaccatt ttttgaaccg ccgccatagc ctgcccggtg
     2221 gaaacgccgg ttgcaggcga agcggacagc ttcatcgaag gataaccgtt gaagcgtacg
     2281 ctctgttccg taccgttttc ccaagaaaca gtagcaatgg tggaaagcgg tacggcgacg
     2341 ccggatttgt tcggcacggt caggttcaaa atatcggcag gctgcatacg ggcatcctcg
     2401 tcggcctgca ccatcacgcg ttgcagacgg ccttggttcg ggaagtcgct gacataagac
     2461 gaactcagcg cacttgccaa tgcggtgcgg atgtcggcaa acgaaatgcc ttgcgccgcc
     2521 gcggcggcac ggttgatgtc gattttcaac tgcggcgagt cttccaaacc gccagcacgg
     2581 acggtgctgg ggtcaaacaa accgctggca cgcattttct gaatcaactc gttgcgcttc
     2641 gccagcaatg cggtatggcc ggtattattg cggtcttgca ggttgatgct caggcccgaa
     2701 ccattgccca actccagaat cggaggcggg acgacggcga tgccaaaacc gtctttaagc
     2761 gtccccatca tcatacccgt cagcttgccg gcaatcgcaa cggcatcgct gccgggcgcg
     2821 gtacgctcgt tccaatcttt caatatggca aaacccatcg ccatattctg accgctgccc
     2881 gaaaagctga agccggaaac ggtaatgatg ttttctattt caggaatgct tttcgccagt
     2941 tgggtaactt gcgccaaagt cgcattggtg cgctcttggg tcgctcctgc aggcagttgc
     3001 acgctgacca tgacgaagcc ttggtcttcg gtcggcagga atgaagtcgg caggcgcata
     3061 aacaggaaca cgcccacaac cgccaagccg atatagacaa ccatcatgcg gaaagtctta
     3121 cgcagcactt tggcaacccg gccttcgtaa ccgtgcgtcc aactgttgaa tttcttgtta
     3181 aaccagccga agaaaccttt tttctcttcg tgatgccctt tcgggattgt cttcaacatt
     3241 gtggcacaca aagcaggggt aagggtcagc gcaaggaagg cggagaatgc gattgatgac
     3301 gccatcgtca gggcaaactg tttgtaaata ttgcccgtcg ccccgctgaa catcgccaac
     3361 ggtacgaaca cggaaatcag aacggcggta ataccgatga ccgcgcccga aatctgaccc
     3421 atcgcttttt tggtcgcttc tttgggcggc aagccttcac ccgccataat gcgctcgacg
     3481 ttttcaacca ccacaatcgc gtcatcgacc acgatgccga tgaccaaaac catcgcaaac
     3541 atggtcagta cgttaatcga catgcccata taagagatga aggcgaaacc gcccaacagc
     3601 gaaatcggta cgacgatggt cggaatcagc gtataacgga tgttttgcag gaagagatac
     3661 attacgacaa acaccagcac catcgcttcg attaaagtgt gaatcacttt ttcaatcgaa
     3721 atttcgacga atttggaagt atcgtaaggg gttttccagc tcataccctg aggaaagtat
     3781 ttttccaacg tcgccatgcg ttctttaacc gcctttgccg tcgccatcgc attgccgctg
     3841 ttggacagca tcaccgccat accggtggta tttacaccgt tcagacgggt tgaggaagaa
     3901 tagtcttcca tacccagtcc gacccttgcc acatccttca ggtaaacatt agaaccgtcg
     3961 gtattggcgc ggaggatgac gttgccgaat tcttctgccg tacccaactg cccttgcgcc
     4021 gttacggtag ccgtaaccgt ctgtccgcga acggcgggaa gcgaaccgat agaacccgct
     4081 gaaatctgga cgttctgggc ggacagcgcg ctgccgatat cggcaaacga caaattgtag
     4141 ttttgcagtt tcttaggatc aacccaaatc cgcatcgcgc gttgcgcgcc gaacaggcgt
     4201 acctgcccca cgccttcgat acgctgcaac tcgggaacga tattacgctg cgcgtagtcg
     4261 ttcatctctt cggttgactg cacatccgac gaaagcatca caatcatcag gaaattggaa
     4321 cgcgccttgg atacggttac gccgtattgc tggacggttg ccggcagcgt gctcaatact
     4381 tcggaaagct tgttctgcac ttccacctgc gccagattct cgtcggtatc gggcgtaaag
     4441 gtcaggctca cgctgccgct gccgctcgaa tcggcggaag tggacatata atccaaacct
     4501 tccacgccgt tcatattccg ctcgatcacg gaaagcacgc tgtcttccat tacctgcgcg
     4561 gacgcacccg gataagtggc gtgcaaagta atggtcgggg cggcgacaga cggatattgc
     4621 gaaacaggca ggcttttgat gccgaaaata cccgccgcaa taatgaaaat cgaaataacc
     4681 cacgcaaaaa tagggcggtc gataaaaaat ttagccatcg atgccttcct tattccgctt
     4741 cagaagcagt tttggcttca gatgccgtct gaacgccgga ttgaggcgcg gcggcttggt
     4801 tttcagacgg cgcccattct ttgggcgtta cctttttcgc acccgttata ctggcgatac
     4861 tgatgccttc cacaaccacc ttgtccccgt ccttcagacc cgacgtaaca atccaattcg
     4921 taccctgctg ttgcgcaacc gttacctcgc ggggttccat accgccttgg gcattcacaa
     4981 tcatcacggt atctttcgca ccgcgcgtta ccgcctgctg cggcacaaca aatgcgttat
     5041 ccaccgccac ttggtccatc agcacgcgca catacagacc gggcatcagg atattctgat
     5101 cgttcggtac ggcggcgcgc agggtaatct gaccggtcga ttcgttgacg accggatcgg
     5161 caaacagcag gcggcctttt tcagggtaaa ctgtgccgtc gtcaaatttg atgccgaccg
     5221 caatcacacc atccgccgcc agcagtttgc cttcggctat ctgacggcgc aatttcatca
     5281 cttcggatgc agactgggta acgttcacat acatcggatt ggtttggcgg atggttgcca
     5341 gcacggtcgt atcgcccgca ttcaacagcg taccttcgga aactttagac tgaccgataa
     5401 agccggaaat cggcgcggta atgcgcgaac ggttcagatt gatgccggca gatttgatcg
     5461 ccgcctgtgc tgctttgaca cctgcctcgg cagaacgttt cgccgttacc gcagcatcgt
     5521 attcctgccg gctgacggct tcggcggcaa ccaaaggctt gtatcgcgcc aaatccgcat
     5581 ccgctttggc aagcgttgcc tgagccgttg ccagttgcgc gcgcgcgctt tccagacctg
     5641 cttcataagt ggaactgtcg atctgataca gcggctgttc ggcacggaca taactgcctt
     5701 cttggaacag gcgtttttgg atgatgccgc cgacttgggc gcggacatcg gcggtacgca
     5761 gcgattccaa acgccccggc aactcgacgg tcaacgcgac ggtttgcgga tggacggtta
     5821 cgacaccgac gacgggcgca ggggcttccc gaccagcagg ctgcccgccc tgcgccgcgt
     5881 ctccgccttt accgcaagac gacagtacca atgcaacggc ggcagccaac gcggccgcac
     5941 gcatcgcctt aaaagcataa aaagccatta tttatcctat ctgtctggtt tgatgtaaag
     6001 ggttttgcca atcaacaggc attc
//
LOCUS       DAXHNE010000120         6011 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.120, whole genome shotgun sequence.
ACCESSION   DAXHNE010000120 DAXHNE010000000
VERSION     DAXHNE010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.120"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..419)
                     /locus_tag="ACFM4Z_001638"
     CDS             complement(<1..419)
                     /locus_tag="ACFM4Z_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFC6021648.1"
                     /translation="MLCPEKMSGMAGQYPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASQQELEALPGIGPAKAKAIAE
                     YRAQNGAFKSVDDLTKVKGIGPAVLAKLKDQASAGAPA"
     gene            complement(455..568)
                     /gene="rrf"
                     /locus_tag="ACFM4Z_001639"
     rRNA            complement(455..568)
                     /gene="rrf"
                     /locus_tag="ACFM4Z_001639"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(665..3556)
                     /locus_tag="ACFM4Z_001640"
     rRNA            complement(665..3556)
                     /locus_tag="ACFM4Z_001640"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3962..4037)
                     /locus_tag="ACFM4Z_001641"
     tRNA            complement(3962..4037)
                     /locus_tag="ACFM4Z_001641"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4002..4004),aa:Ala,seq:tgc)
     gene            complement(4043..4119)
                     /locus_tag="ACFM4Z_001642"
     tRNA            complement(4043..4119)
                     /locus_tag="ACFM4Z_001642"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4083..4085),aa:Ile,seq:gat)
     gene            complement(4221..5761)
                     /locus_tag="ACFM4Z_001643"
     rRNA            complement(4221..5761)
                     /locus_tag="ACFM4Z_001643"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1305 a         1763 c         1293 g         1650 t
ORIGIN      
        1 gcgggcgcgc cggcagaagc ctgatccttc agcttcgcca gcaccgcagg gccgatgccc
       61 tttaccttgg tcaaatcgtc tacagacttg aacgcaccgt tttgcgcacg gtattccgca
      121 atggccttcg ccttcgccgg gcctatgccc ggcagcgcct ccaactcctg ctgcgaagcc
      181 gcattgatgt ttaccgccgc aagggagaag gcgcaggaga acagcataca gaacagcacg
      241 aacattttct tcatggtttt tcctttaagg gttgcaaaca ataaaccgca tcttgcgacg
      301 ataaaacgag tcattctaaa atgaatatcc caaagtttca agccgttcct ccgcaaaccc
      361 gaccggacgc cgtacggata ctgtcccgcc atccccgaca ttttttccgg gcaaagcaaa
      421 aacccccgaa taatcggggg ttttctgaat gggtgtttgg cagtgaccta ctttcgcatg
      481 gaagaaccac actatcatcg gcgctgagtc gtttcacggt cctgttcggg atgggaaggc
      541 gtgggaccaa ctcgctatgg ccgccaaact taaactgtta caaatcggta aagccttaat
      601 caatatattc ggtaatgact gaatcagtca gtaagctttt atctcttgaa gttcttcaaa
      661 tgatagagtc aagcctcacg agcaattagt atgggttagc ttcacgcgtt accgcgcttc
      721 cacaccccac ctatcaacgt cctggtctcg aacgactctt tagtgcggtt aaaccgcaag
      781 ggaagtctca tcttcaggcg agtttcgcgc ttagatgctt tcagcgctta tctcttccga
      841 acttagctac ccggctatgc aactggcgtt acaaccggta caccagaggt tcgtccactc
      901 cggtcctctc gtactaggag cagcccccgt caaacttcca acgcccactg cagataggga
      961 ccaaactgtc tcacgacgtt ttaaacccag ctcacgtacc actttaaatg gcgaacagcc
     1021 atacccttgg gaccgactac agccccagga tgtgatgagc cgacatcgag gtgccaaact
     1081 ccgccgtcga tatgaactct tgggcggaat cagcctgtta tccccggagt accttttatc
     1141 cgttgagcga tggcccttcc atacagaacc accggatcac tatgtcctgc tttcgcacct
     1201 gctcgacttg tcggtctcgc agttaagcta ccttttgcca ttgcactatc agtccgattt
     1261 ccgaccggac ttaggtaacc ttcgaactcc tccgttacgc tttgggagga gaccgcccca
     1321 gtcaaactgc ctaccatgca cggtccccga cccggatgac gggtctgggt tagaacctca
     1381 aagacaccag ggtggtattt caaggacggc tccacagaga ctggcgtctc tgcttctaag
     1441 cctcccacct atcctacaca agtgacttca aagtccaatg caaagctaca gtaaaggttc
     1501 acggggtctt tccgtctagc agcgggtaga ttgcatcttc acaaccactt caacttcgct
     1561 gagtctcagg aggagacagt gtggccatcg ttacgccatt cgtgcgggtc ggaacttacc
     1621 cgacaaggaa tttcgctacc ttaggaccgt tatagttacg gccgccgttt actggggctt
     1681 cgatccgatg ctctcacatc ttcaattaac cttccagcac cgggcaggcg tcacacccta
     1741 tacgtccact ttcgtgttag cagagtgctg tgtttttaat aaacagtcgc agccacctat
     1801 tctctgcgac cctcctgggc ttacggagca agtccttaac cttagagggc ataccttctc
     1861 ccgaagttac ggtatcaatt tgccgagttc cttctcctga gttctctcaa gcgccttaga
     1921 attctcatcc tgcccacctg tgtcggtttg cggtacggtt cgattcaaac tgaagcttag
     1981 tggcttttcc tggaagcgtg gtatcggtta cttcgtgtcc gtagacactc gtcgtcactt
     2041 ctcggtgtta agaagacccg gatttgccta agtcttccac ctaccggctt aaacaagcta
     2101 ttccaacagc ttgccaacct aaccttctcc gtccccacat cgcatttgaa tcaagtacag
     2161 gaatattaac ctgtttccca tcgactacgc atttctgcct cgccttaggg gccgactcac
     2221 cctacgccga tgaacgttgc gcaggaaacc ttgggctttc ggcgagcggg cttttcaccc
     2281 gctttatcgc tactcatgtc aacattcgca cttctgatac ctccagcaca ctttacaatg
     2341 caccttcatc ggcctacaga acgctcccct accatgccgg taaaccggca tccgcagctt
     2401 cggttataga tttgagcccc gttacatctt ccgcgcagga cgactcgacc agtgagctat
     2461 tacgctttct ttaaatgatg gctgcttcta agccaacatc ctggctgtct gggccttccc
     2521 acttcgttta ccacttaatc tatcattggg gaccttagct ggcggtctgg gttgtttccc
     2581 tcttgacaac ggacgttagc acccgctgtc tgtctcccga ggaaccactt gatggtattc
     2641 tgagtttgcc atgggttggt aagttgcaat aaccccctag ccataacagt gctttacccc
     2701 catcagtgtc ttgctcgagg cactacctaa atagttttcg gggagaacca gctatctccg
     2761 agtttgttta gcctttcacc cctatccaca gctcatcccc gcattttgca acatgcgtgg
     2821 gttcggtcct ccagtacctg ttacggcacc ttcaacctgg ccatggatag atcactcggt
     2881 ttcgggtcta cacccagcaa ctcatcgccc tattaagact cggtttccct acgcctcccc
     2941 tattcggtta agctcgctac tgaatgtaag tcgttgaccc attatacaaa aggtacgcag
     3001 tcacaccact agggcgctcc cactgtttgt atgcatcagg tttcaggttc tgtttcactc
     3061 ccctcccggg gttcttttcg cctttccctc acggtactgg ttcactatcg gtcgatgatg
     3121 agtatttagc cttggaggat ggtcccccca tattcagaca ggatttcacg tgtcccgccc
     3181 tacttttcgt acgcttagta ccgctgttga gatttcgaat acgggactgt cacccactat
     3241 ggtcaagctt cccagcttgt tcttctatct cgacagttat tacgtacagg ctcctccgcg
     3301 ttcgctcgcc actacttgcg gaatctcggt tgatttcttt tcctccgggt acttagatgg
     3361 ttcagttctc cgggttcgct tctctaagtc tatgtattca acttaggata ctgcacagaa
     3421 tgcagtgggt ttccccattc ggacatcgcg ggatcattgc tttattgcca gctcccccgc
     3481 gcttttcgca ggcttacacg tccttcgtcg cctatcatcg ccaaggcatc cacctgatgc
     3541 acttattcac ttgactctat catttcaaga acttctttga ctttgcctaa cattccgttg
     3601 actagaacat cagacttgaa tttcctactt tgataaagct tactgctttg ttgtgtctta
     3661 atcctgcctt ttgtgtttca ggattaagtc gatacaatca tcacccaaat actgtgtttg
     3721 ttttcttttc tctcgcgaga gatttttatc ctttgcaaag aataaaaaat caaaacaaac
     3781 gctttgtctt tgtttgttga tttcggcttt ccaatttgtt aaagatcgat gcgttcgata
     3841 ttgctatcta ctgtgcaaat caaaacgagc tgattattat atcagcattt tgttcttggt
     3901 caagtgtgac gtcgccctga atggattctg ttccattctt ccgttttgat ttgtacagta
     3961 ttggtggagg caaacgggat cgaaccgatg accccctgct tgcaaagcag gtgctctacc
     4021 aactgagcta tgcccccgtt cttggtgggt ctgggaggac ttgaacctcc gaccccacgc
     4081 ttatcaagcg tgtgctctaa ccagctgagc tacaaacccg gattctcttc ttaagcgaat
     4141 cttgccttca ctcaagcttc ttccgcatct ttttcagttt accgataagt gtgaatgcct
     4201 aaagcctctt ctttctctag aaaggaggtg atccagccgc aggttcccct acggctacct
     4261 tgttacgact tcaccccagt catgaagcat accgtggtaa gcggactcct tgcggttatc
     4321 ctacctactt ctggtatccc ccactcccat ggtgtgacgg gcggtgtgta caagacccgg
     4381 gaacgtattc accgcagtat gctgacctgc gattactagc gattccgact tcatgcactc
     4441 gagttgcaga gtgcaatccg gactacgatc ggttttgtga gattggctcc gcctcgcggc
     4501 ttggctaccc tctgtaccga ccattgtatg acgtgtgaag ccctggtcat aagggccatg
     4561 aggacttgac gtcatcccca ccttcctccg gcttgtcacc ggcagtctca ttagagtgcc
     4621 caactgaatg atggcaacta atgacaaggg ttgcgctcgt tgcgggactt aacccaacat
     4681 ctcacgacac gagctgacga cagccatgca gcacctgtgt tacggctccc gaaggcactc
     4741 ctccgtctcc ggaggattcc gtacatgtca agaccaggta aggttcttcg cgttgcatcg
     4801 aattaatcca catcatccac cgcttgtgcg ggtccccgtc aattcctttg agttttaatc
     4861 ttgcgaccgt actccccagg cggtcaattt cacgcgttag ctacgctact aagcaatcaa
     4921 gttgcccaac agctaattga catcgtttag ggcgtggact accagggtat ctaatcctgt
     4981 ttgctaccca cgctttcggg catgaacgtc agtgttatcc caggaggctg ccttcgccat
     5041 cggtattcct ccacatctct acgcatttca ctgctacacg tggaattcta cctccctctg
     5101 acacactcga gtcacccagt tcagaacgca gttcccgggt tgagcccggg gatttcacat
     5161 cctgcttaag taaccgtctg cgcccgcttt acgcccagta attccgatta acgctcgcac
     5221 cctacgtatt accgcggctg ctggcacgta gttagccggt gcttattctt caggtaccgt
     5281 catcagccgc tgatattagc aacagccttt tcttccctga caaaagtcct ttacaacccg
     5341 aaggccttct tcagacacgc ggcatggctg gatcaggctt gcgcccattg tccaaaattc
     5401 cccactgctg cctcccgtag gagtctgggc cgtgtctcag tcccagtgtg gcggatcatc
     5461 ctctcagacc cgctactgat cgtcgccttg gtaggccttt accccaccaa ccagctaatc
     5521 agatatcggc cgctcgaata gcgcaaggcc caaaggtccc ctgctttctc tctcaagacg
     5581 tatgcggtat tagctgatct ttcgatcagt tatcccccac tactcggtac gttccgatat
     5641 gttactcacc cgttcgccac tcgccacccg agaagcaagc ttctctgtgc tgccgtccga
     5701 cttgcatgtg taaagcatgc cgccagcgtt caatctgagc caggatcaaa ctcttatgtt
     5761 caatctctaa ctttttaact tctggtctgc ttcaaagaaa ccaacaggac aatgttcaaa
     5821 acattatctt gtctgtcttt caaacagtgt gaggctcaag gcactcacac ttatcggtaa
     5881 tctgttttgt taaagagcgt ttgaattata aagtatttct tccgcctgtc aagatatctc
     5941 tcgatatccc caacattctg tgctatactt ttcagttcgc ccgccgcttc tgcagcagcg
     6001 aagaaccgaa c
//
LOCUS       DAXHNE010000121         6003 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.121, whole genome shotgun sequence.
ACCESSION   DAXHNE010000121 DAXHNE010000000
VERSION     DAXHNE010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.121"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(54..362)
                     /locus_tag="ACFM4Z_001644"
     CDS             complement(54..362)
                     /locus_tag="ACFM4Z_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021649.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHNHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLADLDKELC
                     "
     gene            complement(346..648)
                     /locus_tag="ACFM4Z_001645"
     CDS             complement(346..648)
                     /locus_tag="ACFM4Z_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFC6021650.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(722..3085)
                     /gene="pheT"
                     /locus_tag="ACFM4Z_001646"
     CDS             complement(722..3085)
                     /gene="pheT"
                     /locus_tag="ACFM4Z_001646"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFC6021651.1"
                     /translation="MQFSYSWLKTQADTELSSDKLEHLLTMSGLEVEEAETAAPAFAG
                     VVIAEVKSVEKHPDADRLNVTRVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSDGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQIDAPADCGRFI
                     SRVIENVNARATTPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSDGRFVQNERIGGLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGNVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3121..3639)
                     /locus_tag="ACFM4Z_001647"
     CDS             complement(3121..3639)
                     /locus_tag="ACFM4Z_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021652.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIMP
                     SAFSNLQWGSIEIKLLLVIPDVPLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(3646..4710)
                     /locus_tag="ACFM4Z_001648"
     CDS             complement(3646..4710)
                     /locus_tag="ACFM4Z_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6021653.1"
                     /translation="MIKSFWLENFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQTTWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(4887..5879)
                     /gene="pheS"
                     /locus_tag="ACFM4Z_001649"
     CDS             complement(4887..5879)
                     /gene="pheS"
                     /locus_tag="ACFM4Z_001649"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFC6021654.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
BASE COUNT         1511 a         1550 c         1274 g         1668 t
ORIGIN      
        1 tattttttag cgtgtaaaac aaaaccggct gcatacctgc aaccggcctc aaatcagcac
       61 aattccttat ccaaatccgc caacaggtct ttcagaccgt ctccgatttc ctgaagcgta
      121 accggacggt tgccgtccga acccgattcc gcaccttctg catacggtgc aaacgtgctc
      181 tgcaatttca acagtttcac cacatccaaa cgggtgtagc gttcgccgcc gtaaccgaca
      241 accacaccgt gattatgctg ccattgcgca aaaccatagg ggctgatttg caacagtccg
      301 cacaactcgt ccagcgtgaa atagcgtttt gcgggaacct tcaaatcaac gttgtttgtc
      361 atagtagtgt tccaccatgc ttttgagttt ctggctggca tggaaagtta ccacgcggcg
      421 ggcggtaatc ggcacttcct cgccggtttt aggattacga cccgggcgtt gcggcttgtc
      481 gcgcaactgg aaatttccga aaccggaaat tttgatttct tcgccgcttg ccaaagtgct
      541 gcggatttct tcaaaaaaga gttcgacgat ttctttggca tcgtttttgg tgacgttgct
      601 gactttgtct accaaaatat cggccagttc tgctttagtg agagtcatat gattaccttc
      661 tgttgtaaca gttgtggatt attaccaatt tttatttaaa attcaagcga atatttattt
      721 tttaactgcg aagccgcgcc cctgccgccg ttgccgcgcc aatcagtttt ccgataagcg
      781 gctcgaccgc ctcatccgtc agcgtgtttt ccatatcctg caaaatcact ttgaccgcca
      841 cgctcttcat cccttcgggc agtcccgtgc cgcgatacac gtcaaacacg ctgatttcct
      901 gtaccaactt gtttgccgcg cctttcaaga caagcagcaa atcatcatgg ctcatagctt
      961 ccggcatcac aaacgccaaa tcgcggcgca ccggctggaa tttcgatacg gcccgatagc
     1021 gcgttttccc gcattccaac acggccgcca tatcgatttc aaataccagc ggcgcttgcg
     1081 gcaggtcgta tttttgcagc catttcggat gcagttcgcc gacaaagccg atgacgttgc
     1141 cgtctgaaac gatattggcg gcgcgtccgg gatgcagggc aggatgtccg gttttaacga
     1201 actcgaccgc tttgtttttc aacagatttt ccacatctgc cttaatgtcg taaaaatccg
     1261 cattgcgcgt tttcccgccc cattgttcgg gcattgccgc gccgtaccac aagccgccga
     1321 tgcgttcgtt ttggacaaac cggccgtctg aacctttgcc gaacacgcgg gcgatttcaa
     1381 acacgcgcac gcggttttgt ttgcggttca ggttgttttg caaaatttcc accaagccgc
     1441 cgatgagtgt ggaacgcatc acggcatatt gcgccgccag cgggttttgc aggcggatgg
     1501 ggtcggcgtt ggcggcaaaa tcttgttccc actgctcgtc aacgaaggca tagctgacca
     1561 cttcgcggta gccgcgcgcc gccatttcgt tgtaaacggc aaaacgcggg cggcgtgttt
     1621 cgggcagttc cagcattttc agacggcctg acgtgtaatc gtcggggatg ttttcatagc
     1681 cgtaaacgcg tccgatttct tcaatcaaat cagcctcaat ttcgatgtca aaacggaagc
     1741 tcggcgcggt aacgcggaag ccttccgccg ttttctcggg ctgcaggccc aagtgttgca
     1801 aaatggtttc cacctgttcg gcaggaatgt ccacgcccaa cacggttttc agacggccca
     1861 aacgcaatcc gacctgcttc gcttcgggca attcgccttg cgcttccacc atttcgcctg
     1921 ccgcaccgcc gcaaatctgc aacaccaatt cggtagcacg ctcaatggca tccgcctgca
     1981 aacggtaatc cacgccgcgc tcgaagcgga acgacgaatc cgaaccgaaa ccgtattggc
     2041 gcgatttgcc ggcgatgatt tcgggcgcaa accaagccgc ttccagcacg atattttgcg
     2101 tgccgtctga aaccgcgctc gccgcgccgc ccattaagcc cgccaaactc aacacgcctt
     2161 tttcgtccgc cacgaccagc gtgttttcag acagggaaac ggttttctcg ttcaggcatt
     2221 ccagcgtttc cccttcgcgc gcgcggcgga tgtgcaggct gccggaaagt ttgtcggcat
     2281 caaaaacgtg catcggctga ccgatttcca gcatcacata attgccgatg tccaccagcg
     2341 cggaaatact gcggatgccg ctgcgctcca aacgttgttt catccaatcc ggcgtagtag
     2401 cgcgcgcgtt cacgttttca atcacacggc tgataaagcg accgcaatcg gcaggcgcgt
     2461 caatctgcac gggctgtttt cgactgcccg tgatcggcgc ggtatggatt tcgggctgcc
     2521 tgaacgcgca ccccgtcaat gcggacactt cgcgcgcaat gcctttgatg ctcaagcagt
     2581 cggcgcggtt aggcgtaatt ttcaacgtaa acagcgtatc gtccaaatcc aagtattcgc
     2641 ggatattggt accgacgggc gcatcttcag gcagaatgtg caggccgttc acaccgtcgt
     2701 cgggcagacc gagttcgtcg gtggaacaca acatcccgtc cgacaccacg ccgcgcattt
     2761 tggtcggctt gattttgaaa ttgcccggca acactgcgcc cggcaacgaa cacggcactt
     2821 tgatgcccgc tttcacattc ggcgcaccgc acacaatctg caccaacccg cccgtacccg
     2881 catcaactcg ggtaacgttc aaacggtctg catccggatg tttttcaacg gatttcactt
     2941 cggcaatcac cacgcccgca aacgcaggcg cggcagtttc ggcctcttcc acttccaagc
     3001 cggacatcgt taacagatgt tccagcttat cggaggaaag ttcggtatcg gcttgggttt
     3061 tcagccatga ataggagaat tgcatgttat gttctcaatt aactaattaa ctttagattt
     3121 tcaagagcta gctaaacctt tttgaatggc taagtcccta ttaattactt ctacagaaat
     3181 gttccataaa gaaatcaacc gcaaagtatc tttcttatta aattcttgct ttattaattc
     3241 attcaattgc ccaagataat taagcggcac atccgggata accagtaata atttgatttc
     3301 tatgctaccc cattgcaaat ttgaaaaagc actaggcata attgtttctt tgatatcttc
     3361 tctgcctaat attcccaaaa tttcaacatt taatgcatta caccattttt gataaatctc
     3421 tgaaatatat tcatcaaaac gctcaggaga tctttcttga tttggaatac tttgttttgc
     3481 ctcaacaaat aacaaattat cttcttgcag atagataaac tctactgtct taataccttc
     3541 ttgccttctc ttccggatat atggttctat ataaaacagg ttatttttta ctagattaaa
     3601 agttatacct gattcagtga gatttattat ttcactcata aaaacctaac caatccatgc
     3661 tgtttccgga gataaaacac ctatttcttc tctgaacaat cgaatagact caccaataat
     3721 ttcattatcc ggcatgctat tttgtaaatt ttcctgcacc caagtagtct gttccttttc
     3781 tttgccaggc ataaaaacag gaatactttc actatgcttt ctagctaata aacacagctt
     3841 cttgataaca aaatatgaat gcgtactcat aaaaatctgt attcccaatt tggatttaga
     3901 taaggaaaac agaatttcta gaaattgatt aattgctttc ggatgtagat tagcttctgg
     3961 ctcatcaata aataagattg atccaggact caaaaaccga tttcctaata gagtgtctaa
     4021 tatcccaatc ttttttaccc cctctgctgt agaattgata gagaaagttt gtctatcttt
     4081 ttcatatacc catgccttct ttttatcatt gtagctaaca taaccgccaa acaatgcttc
     4141 tagatccttt ctagcttccg ctgcctcagg gtaattcctc ccttttgttg gtttaatatc
     4201 caatgctttt accaaatcaa tgtaagtagc atcaaaacca aatctctttt cttcctctga
     4261 ttttttaatt acatcaaata gtgatagaac ttctttgggt ggtaaaaaaa tagagttagc
     4321 gtttattctg ggagaaagtt cgtatagctc cggtttaatt agctttttgg tagttctacc
     4381 aaaagaaaac aaaactgaag aactatcttc taaagtcata cttaacttaa gaggccgttc
     4441 gtttggattt tcttttcctc ttgtaactaa atcaccaatt tcctctactt ggaacgtcca
     4501 atatagctta tctcgcaatt cctcagaaaa atcacgaata ttctttcctt tatgagcttc
     4561 ctcatgactt tttaaaacac tatataatgc ctttaaaata aaagttttcc ctttgctatt
     4621 ttctgcaata attaaattta ttttccctaa attatcagcc ttcaataagc taatcggccc
     4681 gaaattttca agccaaaatg atttaatcat ataatccccc taatatatat caacaatatt
     4741 tgtacaagac acattgtgaa aagacagcaa ccacagcacc atagtaaacg catagcaggc
     4801 ggataatgga gcgacatagc gtttcatgat ttagctttct gaggtaggca caggctgctt
     4861 ttttcatttt taggcagcct acacaatcat tttacaaact gcttcaaaaa attcaaatcg
     4921 ttatcaaaaa acaagcgcaa gtcgttcacg ttgtaacgca gcatagcgaa gcggtcgaga
     4981 ccaataccaa aggcgaaacc ggtatatttt tcagggtcga tattgacgtt tttcaacacg
     5041 ttaggatgta ccataccgca accgccgact tccagccatt tgccgttttc gcccataatg
     5101 tcgatttcgg cagacggttc ggtgaacggg aagaaagacg ggcggaaacg tacttgcaaa
     5161 tcatcgcgtt caaagaagcg acggataaaa tccgtgaaca ctgctttcaa atcggcaaaa
     5221 gttacgccct cttctaccca caaaccttcc gcctgatgga acataggcga gtgcgtggca
     5281 tcgctgtcca cacggtaaac gcggccgggg gcgataatgc ggatgggcgg ctctttttta
     5341 tcgagcatat agcggatttg aatcggggaa gtgtgcgtac gcaaaacatc gccgttttca
     5401 acgtaaaacg tatcctgcat cgcacgggca ggatggtttg cagggatgtt cagggcttgg
     5461 aaattgtgaa aatcgtcttc gatttcaggc ccgtccgcca cttcgaaacc cattccgtga
     5521 aagagttcga ccacacgttg caaggtcagg gttacgggat gcaggctgcc gccttcctga
     5581 gcgcgtccgg gcagggtaat gtcgagggct tcggcggcaa gtcgggcttg cagcttggct
     5641 tcgttgaggg catcgcgttt ggcattaaaa gccgtctgaa accggttttt gcattcattg
     5701 atatgcgcac ctatggtttt gcgctcttca ggcgacattt gccccaaagt tttcagaagt
     5761 ccggtcaact cgccggtttt gccaagataa cgggctttga tttgttctag agcgttgaag
     5821 tcttgcgcag cttctactgc ggcaatgcct tctgcaacga tgcggtttac attttccata
     5881 atatcaagtc tgtcaattaa tgatatacca cctgaaacaa cgaggccgcc tgtatagcct
     5941 gacatatcag actcttcaga cgaccttatt ttcttcacgg tcaatccatt ggataaattt
     6001 tct
//
LOCUS       DAXHNE010000122         5929 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.122, whole genome shotgun sequence.
ACCESSION   DAXHNE010000122 DAXHNE010000000
VERSION     DAXHNE010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.122"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(145..1062)
                     /gene="thrB"
                     /locus_tag="ACFM4Z_001650"
     CDS             complement(145..1062)
                     /gene="thrB"
                     /locus_tag="ACFM4Z_001650"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFC6021655.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPMAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSTEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(1099..1827)
                     /gene="ubiG"
                     /locus_tag="ACFM4Z_001651"
     CDS             complement(1099..1827)
                     /gene="ubiG"
                     /locus_tag="ACFM4Z_001651"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFC6021656.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHAELRGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVVETKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            1979..3220
                     /locus_tag="ACFM4Z_001652"
     CDS             1979..3220
                     /locus_tag="ACFM4Z_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter"
                     /protein_id="HFC6021657.1"
                     /translation="MPNKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFAGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSALGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKMPVLFDTQAPVGTNYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            3353..3916
                     /gene="gmhB"
                     /locus_tag="ACFM4Z_001653"
     CDS             3353..3916
                     /gene="gmhB"
                     /locus_tag="ACFM4Z_001653"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFC6021658.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENTAPQAD"
     gene            3945..4688
                     /locus_tag="ACFM4Z_001654"
     CDS             3945..4688
                     /locus_tag="ACFM4Z_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC6021659.1"
                     /translation="MLIIRNLIYWLILCSSLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            4685..5377
                     /locus_tag="ACFM4Z_001655"
     CDS             4685..5377
                     /locus_tag="ACFM4Z_001655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFC6021660.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHGRQLAPTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPASSSLTSAKTFWGV
                     CRKTTGIRLNWRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYADEAKQW
                     LKDRGRELFALG"
     gene            5416..5583
                     /locus_tag="ACFM4Z_001656"
     CDS             5416..5583
                     /locus_tag="ACFM4Z_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021661.1"
                     /translation="MPEQARARPIQTAMTLCRRFGAGWRHTRRLPRFISHRSSETRNP
                     PGPTCGMKRLS"
BASE COUNT         1381 a         1912 c         1413 g         1223 t
ORIGIN      
        1 cggcttggtc gccttgtcct gatttttgtt aatccactat aaaatggaaa atacccggct
       61 atcgtctcat tttcgtttta atcagccata aaaatgccgt ctgaaaccct ttcagacggc
      121 atttctgtca aacgccggac gcactcaatc caaactcaac agcaggttgc ggaacgcgtt
      181 cgggtctttg ataaacgtca tctcgcccgc ctgcggaaaa tgaaagtcca acaggcgcga
      241 cacccaaaaa cggatgcagc cggcacgttg ggcggtcggg aaatacgcct tttcttcggt
      301 actcaagggg cgcacgccct cataaccgcc gataaacgcc tttttcaacg cctcatccaa
      361 cttattgtcc gccgtccttg cccaatcgtt gaccgcaatc gccaagtcat acataaaatt
      421 gccccggcag gcataataaa aatcgatgaa gcccgatacc tgaccgccgt caagcaacac
      481 attgtcttta aacaaatcgg cgtggatgat gcccgaagga agatgattgc cgagattgtc
      541 cttcaacgca tcgatttcgg aacacagcag cgcggcatcg tcttgcgaca ggacgggcag
      601 cagccgggcg cacgcctccg tccaccacgc atcgtaacgc gggttttcca tttccaaagg
      661 gaaatcggcg gcggcaaggt gcattttcgc caacatcgca ccggtatgga agcactgttc
      721 cgccatcggc agcgcggtat ccgaaccttt caggcaggca accaaacaag ccggcttgcc
      781 cgccaaaacg gaatcaagcc ggccgtcttt gcgcgcaacc ggcgcggcaa ccgccacgcc
      841 cttcatactc aaatgccggt taagctccag aaaaaacggc agctcttcct gtttcaacac
      901 ttcaaacacg gtcagcacat aacgtcccga agtcgtcgtc agaaaataat tgctgttggt
      961 aatcccctgc gcgatgccct gcagggaaac aaattccccc aaatcgtaac cgctcaggaa
     1021 gccgcgcatt tcatcatcgg aaacactggt atagacagac atatcaactc acttgctcaa
     1081 aaacgggcaa acccgccgtc agaacgccgg acggcaggca aacatataat tcacatcggt
     1141 cgaatcgcac agggcataag tttgcgacaa tacatggtaa gtcataccct tggtttccac
     1201 cacgtccaaa cccgcctgac ggcacattcg cgccagctcg gcaggtgcga tgaatttttt
     1261 ccagtcgtgc gtgcctttgg ggacaaactt caacagatat tccgccgcca caatcagatg
     1321 caggtacgat ttcgggtttt tattgatggt ggaaaaaaac accatgccgt ccggtttgac
     1381 cagcttggca caggcgcgaa cgatggcggc gggatcgggg acgtgttcca tcatttccat
     1441 gcacgttacc acatcgaacg agtgcggttc cgcctcggca aggtcttcca cgcggataca
     1501 ttcgtattcg atatcggcga cattgttcaa agccgcgtgc aggcgggcgg tttccagcga
     1561 ctgctccgcc atgtcgatac cctttacaaa cgccgcgccg cgccgcgcca tactttccgc
     1621 caagatgccg ccgccgcagc ccacatccaa aacccgtttg ccgcgcaact ccgcgtgtcc
     1681 gtcgatataa tccagccgca gcggattgat gtcgtgcaag gttttgaact cgcccgactt
     1741 gtcccaccat ttgtcggcaa tccggctgaa tttggcgatt tccccctcat cgacattata
     1801 ttttttgtcg gacattttcc ctcccatctg acgaaccgcc cactccaaaa cccaagatta
     1861 tactatggaa cacaatgccg tctgaaagcc ttttcgggcg gcgcggcgca aatgccttac
     1921 aaatccttac actttacggc ataatggcgg ctcgcttttt ctggcagaaa gacaaaatat
     1981 gcccaacaaa accccttcac tgttcggcgg cgcgatgatt atcgccggca cggtcatcgg
     2041 cgcaggcatg ctcgccaacc cgaccgccac atccggcgta tggtttgccg gctcgctggc
     2101 cgtgttgctg tacacctggt tttctatgct ttccagcggt ctgatgattt tggaagtcaa
     2161 cacccactac ccccacggcg caagtttcga cacgatggtc aaagacctgc tcggacgcgg
     2221 ctggaacatc atcaacggca tcgccgtcgc cttcgtttta tacctgctca cctacgccta
     2281 catcttcgtt ggcggcgacc tgaccgccaa aggcttaggc agcgccttgg gcggcaatgt
     2341 ttcactcacc gtcggacaac tcgtcttctt cggcatcctc gccttttgcg tatgggcatc
     2401 cgcacgcttg gtcgaccgct tcaccagcgt cctcatcggc ggcatggtat taacctttat
     2461 ttgggcaacc ggcggcctga ttgccgatgc caaaatgccc gtcctgttcg acacccaagc
     2521 ccccgtcggc accaactact ggatttacgc cgccaccgcc ctgcccgtct gccttgcgtc
     2581 attcggtttc cacggcaacg tctccagcct gctcaaatac tttaaaggcg acgcgcccaa
     2641 agtggctaaa tccatctgga cgggcacact gattgcgctg gtaatttacg tcctctggca
     2701 aaccgccatc caaggcaacc tgccgcgcaa cgagttcgcc cccgtgattg ccgccgaagg
     2761 gcaagtctcc gtcctcatcg aaaccctgtc caaattcgcc caaaccggca atatggataa
     2821 aatattgtcc ctgttttcct atatggcgat cgccacctcg tttttaggcg taaccttagg
     2881 tctgttcgac tacatcgccg acatcttcaa atggaacgac agcgtgtccg gccgcaccaa
     2941 aaccgccgcg ctgaccttcc tgccgcctct aatttcctgc ctgctcttcc ccaccggctt
     3001 cgttaccgcc atcggctacg tcggcctggc ggcaaccgtc tggacaggca tcatccccgc
     3061 catgctgctc taccgttcgc gcaaaaaatt cggcgcaggc aaaacctata aagtttacgg
     3121 cggcttgtgg ctgatggttt gggtcttcct tttcggcatc gtcaacatcg ccgcacaggt
     3181 attgagccaa atggaactcg tccccgtatt taaaggataa aggcaaaatg ccgtttgaag
     3241 cccgccggcg gcttcagacg gcattgcctc gttatcacgc ggcacaggcg catcactgcc
     3301 gatatttcag tataatgcca cccccgacct gccccaatcc aaaggaaacg cgatgaaact
     3361 catcattctc gaccgcgacg gcgtcatcaa tcaggaccgc gacgacttcg tcaaatccgt
     3421 tgacgagtgg atacctgtcg aaggcagcat ggatgcggtg gcatttctga cgcaggcagg
     3481 ctacaccgtc gccgttgcca ccaaccaatc cggcatcggg cgcaaatatt ttaccgttca
     3541 aaaccttacc gaaatgcacg ccaaaatgca ccgcctcgtc cgtcaggcag gcggcgaaat
     3601 caacggcatc tggttctgcc cgcacaccga tgccgacaac tgcaactgcc gcaagcccaa
     3661 accgggtatg attgaagaca tcatcggacg cttcaacgcc caagcctcgg aaacctggct
     3721 ggtcggcgac agcctgcgcg atttgcaggc aatcgatgcc gtcggcggca aacccgcgct
     3781 ggttctgacc ggaaaaggca aaaaaacgct ctcccaacac ggacacgaat tgcccgaaca
     3841 cacacaggtt ttcgataccc tgctcgattt ctcacaatac atcatgcagg aaaacaccgc
     3901 accgcaagcc gactgaacat accgcattcc gacaaggcaa aaccatgctc atcatccgca
     3961 acctgattta ctggctgata ctctgttcca gcctgatttt cctctttccc tttatgctgc
     4021 tcgcctcgcc tttccgggac ggggcgcaca agatggcgcg ggtctgggtc aaaatcctca
     4081 acctctcgct caaacacatc gtcgggctca aataccgcat catcggcgcg gaaaacatcc
     4141 ccgaccgccc cgccgtcatc tgcgccaaac accaaagcgg ctgggaaacg cttgcccttc
     4201 aggacatttt tccgccgcag gtttacgttg ccaaacgcga gctgttcaaa atcccctttt
     4261 tcggctgggg tttaaaactg gtcaaaacca taggcataga ccgcaacaac cgccgcgaag
     4321 ccaacgaaca gctcataaaa caggggttgg cgcgcaaaaa cgaaggctat tggattacca
     4381 ttttccccga aggcacgcgc cttgcgcccg gaaaacgcgg caaatacaaa ctcggcggcg
     4441 cgcgcatggc gaaaatgttt gagatggaca tcgtccccgt cgccctcaac agcggcgaat
     4501 tttggccgaa aaactccttt ctgaaatatc cgggggaaat caccgtcgtc atctgtccga
     4561 ccatcccgca cgcaagcggc agcgaagccg aattgatgga aaaatgcgaa cacctcatcg
     4621 aaacgcagca gccgctcatt tccggcgcag gtccgtttgc cgccaaaatg ccgtctgaaa
     4681 ccgcatgacc gcctttgtcc acaccctttc agacggcatg gaactgaccg tcgaaatcaa
     4741 gcgccgtgcc aagaaaaacc tgattatccg ccccgccggc acacataccg tccgcatcag
     4801 cgtcccaccc tgcttctccg tctccgctct aaaccgctgg ctgtatgaaa acgaagccgt
     4861 cctgcggcaa acactggcga aaacaccgcc gccgcaaact gccgaaaacc ggctgcccga
     4921 atccatcctc ttccacggca gacagcttgc ccctaccgcc catcaagaca cgcaaatcct
     4981 gctgatgccg tctgaaatcc gtgttcccga aggcgcaccc gaaaaacagc ttgcgctgct
     5041 gcgggacttt ttggaacggc aggcgcacag ttacctgatt ccccgcctcg aacgccacgc
     5101 ccgcaccaca caactgttcc ccgcctcctc ctcgctgacc tctgccaaaa ccttctgggg
     5161 cgtgtgccgc aaaaccacag gcatacgcct caactggcgg ctggtcggcg caccggaata
     5221 cgttgccgat tatgtctgca tacacgaact ctgccacctc gcccatcccg accacagccc
     5281 cgccttttgg gaactgaccc gccgcttcgc cccctacgcg gacgaagcca aacaatggct
     5341 caaagaccgc ggcagggaac ttttcgcctt aggctgacgc ggaccggacc gacccgccgc
     5401 tttcagacgg catccgtgcc ggaacaggca cgcgcccgcc cgattcaaac cgcgatgacg
     5461 ctttgccgcc ggttcggggc aggatggcgg cacacgcgcc gtctgccgcg tttcatttca
     5521 caccgctctt ccgaaacccg aaacccgccc ggtccgacgt gcggtatgaa acgcttaagc
     5581 tgacgcgaag tcttttactg atttgcccgc gaaaatgccg tctgaaaggt tttcggacgg
     5641 catttttttg cgtttcccag gatggcggcg gattcgtaaa aggcggtcag ggtggattgt
     5701 aggatgggtt gagacctgcc gaatccgccg catctgccaa atctaccgcc gtcattccta
     5761 cgaaagtggg aatctagaac gcggggttaa gaaaacctgc accgcgtcat tcccacgaaa
     5821 gtgagaatcc aggacgcaaa atctcaagaa accttttttc ccgataaatt tccgcaccga
     5881 cagacctaga ttcccgcctg cgcgggaatg acgggatttg aggtttctg
//
LOCUS       DAXHNE010000123         5921 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.123, whole genome shotgun sequence.
ACCESSION   DAXHNE010000123 DAXHNE010000000
VERSION     DAXHNE010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.123"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(41..2575)
                     /locus_tag="ACFM4Z_001657"
     CDS             complement(41..2575)
                     /locus_tag="ACFM4Z_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6021662.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRISLSAANIEAENGK
                     IKIQSYGDQYYYARQSELYTFERRSYKTGKWYNRKHITEVKEHKNAKPDAVTLSASQG
                     IDIKSGGSIDAYATAFDAPKGSINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYS
                     KAHDTTTQVMKTALPSRVVAESANLQSGWDTKLQGTQFETTLGGATIRAGVGEQARAD
                     AKIILEGIKSSIHTETVSSSKSTLWQKQAGWGSNIETLQLPSFTGPVAPVLTAPGGYI
                     ADIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNINWNQVQLAYDRWDYKQEGLTEAGAA
                     IIALAVTVVTSGAGTGAVLGLNGAAAAATDAAFASLASQASVSFINNKGDVGKTLKEL
                     GRSSTVKNLVVAAATAGVADKIGASALNNVSDKQWINNLTVNLANAGSAALINTAVNG
                     GSLKDNLEANILAALVNTAHGEAASKIKQLDQHYIAHKIAHAIAGCAAAAANKGKCQD
                     GAIGAAVGEIVGEALVKNTDFSRMSATEIEKAKAKITAYSKLVAGTASAVVGGDVNTA
                     ANAAQIAVENNTLYPRCVGAKCDEFQKEQQKWIRENPEEYREVLLFQTGFIPIIGDIQ
                     SFVQAQTAADHLFALLGVVPGIGESIQAYKVAKAAKNLQGMKKALDKAATVATAQGYV
                     SKTKIKIGQTELRVTAATDKQLLKAIGEGRDTTGKMTEQLFDSLAKQNGFRVLSGGKY
                     GGNNGFDHVWQAADGSVVLIVESKQIRNGTVQLNPNGAGGYTQMSREWIKQVVKSLPD
                     GSPAKAVVLKANQNGKLKTAIAGVDRQTGKAVILSVKVPSKTNIRR"
     gene            complement(2822..3205)
                     /locus_tag="ACFM4Z_001658"
     CDS             complement(2822..3205)
                     /locus_tag="ACFM4Z_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021663.1"
                     /translation="MEKIKNYIEFKKLFKNITEKFVRDLDITLIEAAEYSIIEFEIYL
                     SEYPEDSTFIFTAWAIYLLENNCLNERGKDNFFIAEMKKCYQQTNLDRIKSIYSSSDY
                     HIFAKDVNKIQQTFKLNLANIMQQE"
     gene            complement(3210..4047)
                     /locus_tag="ACFM4Z_001659"
                     /pseudo
     CDS             complement(3210..4047)
                     /locus_tag="ACFM4Z_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249609.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase domain-containing protein"
     gene            complement(4346..4741)
                     /locus_tag="ACFM4Z_001660"
     CDS             complement(4346..4741)
                     /locus_tag="ACFM4Z_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021664.1"
                     /translation="MNQFCLSEYEKAFLCRIIENNMIWKEYLIEQIENSIIVSRDNTG
                     CGCYIDFQVNLERTNLNKYENIPIFFEGDITAPDKSDAATFLLYTTESLNKAGRIIDF
                     LEVAIAGTNYDEVEVYSWLKMENSLRFGL"
     gene            complement(4753..5466)
                     /locus_tag="ACFM4Z_001661"
                     /pseudo
     CDS             complement(4753..5466)
                     /locus_tag="ACFM4Z_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            complement(5562..5834)
                     /locus_tag="ACFM4Z_001662"
     CDS             complement(5562..5834)
                     /locus_tag="ACFM4Z_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021665.1"
                     /translation="MNKRMKMCPACQQGYLYHSKPKYLHDEIILCDECDAVWLKGMNI
                     FYGEYEKDFYSYVPFMESQGITSECIWEGDLFDHPYYEDENSNDMD"
BASE COUNT         1493 a         1338 c         1160 g         1930 t
ORIGIN      
        1 tcataccatt tttgggtggt catcatattg tgccccattg ttatctcctt atattggttt
       61 tagaaggaac tttgacagaa agaataacgg ccttacctgt ttgacgatca acgcctgcta
      121 ttgccgtttt taatttgccg ttctgatttg cttttaagac aactgcctta gcaggactac
      181 catcaggtaa actttttaca acttgtttaa tccattcacg actcatctgc gtatatccac
      241 ccgcaccatt cggattcagc tgtaccgtac cgttcctaat ctgcttactt tctacaatca
      301 aaacaacact accatcggca gcctgccata catgatcaaa accgttattt ccgccgtatt
      361 tgccgcccga aagcactctg aagccatttt gtttagctaa agagtcaaat aactgctcgg
      421 tcattttacc tgtcgtgtcc cttccttcgc caatagcttt cagcaattgt ttgtcagttg
      481 ctgcagtaac ccttaattca gtttgaccga ttttgatttt ggttttactg acatagccct
      541 gtgcagtggc aacggttgct gccttgtcca aggctttttt catgccttgt aaattttttg
      601 ccgctttcgc tactttatag gcctgtatcg attcaccgat acccggaacc acacccagca
      661 aagcaaacag gtgatcggca gcggtctgtg cttgtacaaa actctgtata tcaccgataa
      721 ttggaataaa tcctgtctga aaaagcaaaa cttctcgata ttcttcagga ttttcacgta
      781 tccatttttg ttgttccttt tgaaattcat cacactttgc accaacgcat ctaggataca
      841 aagtattatt ctccaccgct atctgtgccg cattcgccgc tgtattcaca tccccgccta
      901 caacggcaga cgcagtgccg gcaaccagtt ttgaataggc agtaatcttc gctttagctt
      961 tttcgatttc ggtcgcactc atacgactga aatcagtatt cttaaccaaa gcctcaccaa
     1021 caatctcacc gactgcagcg cctatcgcac catcctgaca cttgccctta ttcgccgccg
     1081 ctgccgcaca gcccgctatg gcatgggcaa tcttatgggc aatgtagtgc tgatccaact
     1141 gtttgatttt actggctgcc tctccatgcg cagtattcac caaagccgca aggatattcg
     1201 cttccagatt gtctttcagg ctgccgccgt tgacagcggt attaatcagt gcggcactgc
     1261 ccgcattggc taggttgacg gtcaggttgt tgatccactg cttatcgctg acattgttca
     1321 gtgccgaagc gccgattttg tcggctacgc ctgcggtagc ggcggcaacc accagatttt
     1381 tcaccgtgct gcttctgccc agctctttca gggttttgcc gacatcgcct ttgttgttga
     1441 tgaacgatac ggaagcctgg ctggccaaag aggcgaatgc tgcatcggtt gcggcggcgg
     1501 ccgcaccgtt taatcccaat acggctccgg ttcctgcgcc tgaggtgacc acggtaacgg
     1561 ccagtgcgat aatcgccgca cctgcttcgg ttaagccctc ctgtttgtag tcccatctgt
     1621 cgtaagcaag ctgcacctga ttccagttga tgtttttcgc aacttgaagt tgtttcaaat
     1681 aagcatactc gggctgtttg gcgagttttt cgatttcggt tttgaggttg cctttgggga
     1741 tgtcggcgat atagccgccg ggagcggtca gtacgggcgc aacgggaccg gtgaaactcg
     1801 gcaattgcaa ggtttcgatg ttactgcccc atcctgcctg tttttgccat agagtagatt
     1861 tgctgctgct cacggtttct gtgtggatgc tgcttttgat cccttcgagg ataatcttgg
     1921 catctgcccg tgcctgctcg cctacgcctg cgcgtatggt tgcgccaccc agtgtggttt
     1981 caaactgtgt gccttgcagt ttggtatccc aacctgattg cagattggca gattctgcaa
     2041 ctacccttga gggcagcgcg gttttcatga cttgggtggt ggtgtcgtgt gctttgctgt
     2101 agctgatgcc gagaaatctg cgcctttttt ggctgtctag tttgtcgtag ttgagctctt
     2161 ctacggcata gagtgtcaat ttccgcccgg cttcgatgtt aatgctgcct ttgggggcat
     2221 cgaatgcggt ggcgtaggcg tcgatgctgc caccagattt gatgtcgatg ccttgggatg
     2281 cgctgagggt tactgcgtcg ggcttggcgt ttttgtgttc tttgacttcg gtaatgtgtt
     2341 tgcggttgta ccatttgcca gttttgtagc tgcggcgttc aaaggtatag agttcgctct
     2401 gtctcgcata gtaatattgg tcaccgtaag attgaatttt gattttgccg ttttcagctt
     2461 cgatgtttgc cgcactcaga ctgatgcggc tgttctcatt ggcatacggt gcgctaatgc
     2521 tcacaccggt tttacccgaa agttctgcag caagcgtagg gggtgtcttc agcacgcacg
     2581 cgtctgggca gtatataggg aatctgaata tttacttgca taacaaatgc cgtctgaaaa
     2641 attgtgagct tttcagacgg cattgagccg taaatcatgg aacgcgtgcg tgctggagca
     2701 cacaccttac gcatggattt taggtttcat gcaggctaca gcttgctgct gcggtataaa
     2761 agtattttta gtgatttact gtttttggta tttttgcccg acggggttaa aaatacagtt
     2821 gctactcttg ctgcatgata ttggcaagat ttaatttaaa tgtttgttga atcttgttta
     2881 catccttagc aaaaatatga tagtcactcg aagaatagat agattttatt ctatccaaat
     2941 tagtttgttg ataacatttt ttcatttccg cgatgaaaaa attatctttt cctctttcat
     3001 tgagacagtt attttctaac agatagattg cccatgctgt aaaaataaat gtactatctt
     3061 ccggatattc agataaataa atttcgaatt caattataga atattcagct gcttcaatta
     3121 atgttatatc taaatccctg acaaattttt ccgttatatt tttaaataat ttcttgaatt
     3181 ctatataatt ttttattttt tccatgattc tattttcctg taaaaatatt taattgaata
     3241 tttggatagc gatttctaaa ctctaaaata acattgctgc aagattgaca ggcctttaat
     3301 tctgtaaata gatcaattct acctgataca ttacgattat ttccgagttg ctgtgctata
     3361 gtttcaagta atttatattc tccatctata tttcttaatg ttcctctagg aggagaggca
     3421 atattatgat atttatcaac agatataggt ttaaaaattt ttgtttcagg taacgaaata
     3481 aatccatgtt cccctttttg gaaagaacta aatgcttcca tcctttgcgg taatccttta
     3541 acatcaattt ttgctatcgc aagattccct atttgtcgat actcttccgg cagtttcatg
     3601 cggactctct ggctttcagc agttaaaagc aggagcactg tcattgctca gataacgggt
     3661 aacaggtgta ggcttggccg atattgcagc ctgtgcagat acagatcctg caaccatcaa
     3721 tgtgccatta acaacctgaa ctgtaccatg cagatattct ccattagaga ttgctttcgc
     3781 tccctgtcca atgttatatc cccctgctgc cacagtagct gctttgacac tgaagccaag
     3841 cggtccgttt aataagtaaa gtgcaggttg tgcggcaaca gctgatttgt aatcaaaaga
     3901 atcataacga ctcaatgttt gataggcttt cgataaagtg ttattaatat ctttggaagg
     3961 aatagtgaga ttacccattg actggggcgt tttcagtatc gaagctgccc aagctgtgtt
     4021 acctccagag gcttggttaa taaaatatgt tacctatgta tattgtttct tcaaaaatca
     4081 accgtattgt ccttgggtga ttttcgtacc agcacaatca gtctgcccta gttgacagat
     4141 cgcgccacac tagccagata cagcgtatca ttggtaaaac tgataatgcg gcaatataaa
     4201 gaaaaacttc aatagtatgt taacgaaatt tgacgcataa aaatacgcta aaaaattttc
     4261 gcttgactaa ttaaaaatgg gattgagcat aaaattttaa taatttatat tattgaaaat
     4321 atctcagatt ttcagtcagt ttattttaca agccgaaccg taggctattt tccatcttta
     4381 accatgaata gacctcaact tcatcataat ttgtgcctgc aatagcaact tccagaaagt
     4441 caataattct acctgcttta ttcaaagact ctgtagtata tagcaggaaa gtcgctgcat
     4501 ctgacttatc cggtgcagtg atgtctcctt caaaaaaaat agggatattt tcgtatttat
     4561 ttagattagt tctttctaaa tttacctgaa aatcaatgta gcacccacat cctgtattat
     4621 ctctagatac tattattgaa ttttctattt gctctattaa atattccttc catatcatat
     4681 tgttttcaat aattctacat aagaaagcct tctcatattc agataaacaa aactggttca
     4741 tatattctac ccttacattt tcaaaatagt tgcaatacga gatactccat tgttgacata
     4801 tacgcgaaca attaccgtta taccattgac cgttacagta actggataat ttcctgtttt
     4861 tgtcagtttt aaagcatcca ctgccttttg cgtcgctaca acagcatttt ccatggagcc
     4921 tgatttttga atcaatgtat taccgatgct tccttgatgc cccttaaaga tgtgatgaac
     4981 tttatttagc caatctacac cgtatctttc agccaatagt cgagaattac tttcacctat
     5041 tttttccatc gaagctaaaa tcctttgagc ataggcagca tcagctgttg aaaaatttgc
     5101 ttcgcccaac aaaagccgta attcctgaat atctcctcgt aaaattgcat ttcttactcc
     5161 cgcttctaat gtggtttgtg gctgaaagag ggaattattc tctaccgcca cagtagccgc
     5221 attcgccgct gtattcacat ccccgcctac aacggcagac gcagtgccgg caaccagttt
     5281 tgaataggca gtaatcttcg ctttagcttt ttcgatttcg gtcgcactca tacgactgaa
     5341 atcagtattc ttaaccaaag cctcaccaac aatctcaccg actgcagcgc ctatcgcacc
     5401 atcctgacac ttacccttat tcgccgccgc tgccgcacag cccgctatgg catgggcaat
     5461 cttgtgggtg gtatatcgct gccagccctc tctgttgata atcttgcgta tgattacgcc
     5521 gtttgatggc taatcgtacc tacgcaggct taaaatttcc atcaatccat atcatttgag
     5581 ttttcatctt cataatatgg atgatcaaac aaatctcctt cccaaataca ttcactcgtt
     5641 ataccttggg attccatgaa aggaacataa gaataaaaat ctttttcata ttctccataa
     5701 aatatattca tacctttgag ccatactgca tcgcattcat cacacagaat aatttcatca
     5761 tgaagatatt taggtttcga atggtagaga tagccttgtt gacaagcagg acacattttc
     5821 attctcttat tcatgattac tccatataat cgatttattt ttgtgggtaa gcagtagtga
     5881 tttgattgct gcctttagtc tgtacaacaa tacgtatata g
//
LOCUS       DAXHNE010000124         5762 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.124, whole genome shotgun sequence.
ACCESSION   DAXHNE010000124 DAXHNE010000000
VERSION     DAXHNE010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.124"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            241..5688
                     /gene="iga"
                     /locus_tag="ACFM4Z_001663"
     CDS             241..5688
                     /gene="iga"
                     /locus_tag="ACFM4Z_001663"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050177927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HFC6021666.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADKIKQRDNAGTIKGYGEHHWKTTGT
                     NSHIGSTAVRLAGNERGANNGQNVTFENNGTLVLDQNINQGAGGLFFKGDYTVKGANN
                     DITWLGAGIDVADSKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVIL
                     NQQADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFEH
                     IRNVDEGARIVNHNTSHASTITLTGKSLITNPNSLSVHSIQNDYDEDDYSYYYRPRRP
                     IPQGKDLYYKNYRYYALKSGGSVNAPMPENGQTENNDWILMGSTQEEAKKNAMNHKNN
                     QRISSFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGANLNGKISVTQGNVLLSG
                     RPTPHARDFVNKSSAQKDAHFSKNNEVVFEDDWINRTFKATEITVNQSASFSSGRNVS
                     NITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALNSFGATQINGNV
                     NLNQNAALVLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLSLADSHIHLNNA
                     SDAQSANQYHTLKINHLSGNGHFHYLTHLAENLGDKVLVKESASGHYQLHVQDKTGEP
                     NQEGLNLFDASSVRDRSHLSVSLANHHVDLGALRYTIKTENGITRLYNPYAENRRRVK
                     PAPSPATNTASQAQKATQTDGAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKRQ
                     QAEAERKSAELAKQKAEAEREARELATRQKAEQERSSAELARRHEKEREAAELSAKQK
                     VEAEREAQALAVRRKAEAEEAKRQAAELARRHEKEREAAELSAKQRVGEEERRQTAQS
                     QPQRRKRRAAPQDYMAASQDRPKRRGHRSVQQNNVEIAQAQAELARRQQEERKAAELL
                     AKQRAEAEREAQALAARRKAEAEEAKRQAAELAHRQEAERKAAELSANQKAAAEAQAL
                     AARQQKALARQQEEARKAAELAVKQKAETERKTAELAKQRAAAEAAKRQQEARQTAEL
                     ARRQEAERQAAELSAKQKAETDREAAESAKRKAEEEEHRQAAQSQPQRRKRRAAPQDY
                     MAASQNRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPLDALEDEN
                     VSESVDTSDKQPQDNTELHEKYENDYEEIPLDALEDEDVSESVDTSDKQPQDNTELHE
                     KVETVSLQPRAAQPRAQAATQPQAQAAAQADAVSTNTNSALSDAMASTQSILLDTGAS
                     LTRHIAQKSRADAEKNSVWMSNTGYGRDYASAQYRRFSSKRTQTQIGIDRSLSENMQI
                     GGVLTYSDSQHTFDQASGKNTFVQANLYGKYYLNDAWYVAGDIGAGSLRSRLQTQQKA
                     NFNRASIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGNDSVKVSSMSVKTLT
                     AGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGNSFVYKADNQQQYSAGAALLY
                     RNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
BASE COUNT         1940 a         1378 c         1294 g         1150 t
ORIGIN      
        1 aagcgggaat ccaaaaacaa aaagccgcag aaatttaacg gaagaaccga accccgaaaa
       61 ccggcttccc gccttcgctt gaataacaga acaaaggaag cggaaataaa aacatcggga
      121 ttcttttttt aaattggcaa taaaacacca aaatgaatga tttacacaac aaaaattagt
      181 caacaccgcc aaaccggcgt aaaatacaaa acattatcac ccttaaaacg gtaaaacctt
      241 atgaaaacca aacgttttaa aattaacgcc atatccttat ccatctttct tgcctatgcc
      301 cttacgccat actcagaagc agcattggtc agagacgatg tcgattatca aatattccgt
      361 gactttgcag aaaacaaagg caaatttttt gtcggcgcaa ccgatttatc agtgaaaaac
      421 aaacaaggtc aaaacatcgg caacgcatta tccaacgtac cgatgattga ttttagcgtt
      481 gcagatgtca acagacgtac attaaccgtc atcgatcccc agtatgccgt cagcgtcaaa
      541 catgtaaaag gagacgaaat ctcttattac gggcatcaca atgggcactt agatgttagc
      601 aatgatgaaa acgaatatcg ttcagttgca caaaatgact acgaaccaaa taaaaattgg
      661 catcacggta atcaaggtcg tcttgaagac tacaacatgg cacgccttaa taaattcgta
      721 acagaagtcg cacctattgc accaaccagt gcaggcggcg gcgttgaaac ctataaagat
      781 aaaaaccgtt tttctgagtt tgtgcgagtt ggagcaggta cgcaatttga atataacagc
      841 cgctacaata tgacagagct ttcacgagct tatcgttatg ccattgcagg tacgccttat
      901 caagacgtga atgtcacatc gaatctaaat caagaaggct tgattggctt cggtgataat
      961 tcaaaacatc attccccaga aaaactcaaa gaagttttat ctcaaaacgc attgactaac
     1021 tatgctgtgt taggcgatag cggttcacca ttatttgctt atgataaaca agaaaaacgc
     1081 tgggtctttt taggtgctta cgattattgg gcaggctatc aaaaaaactc ttggcaagaa
     1141 tggaatatct ataaaaaaga atttgcagat aaaatcaaac aacgcgataa cgccggcacc
     1201 atcaaaggtt atggcgaaca tcattggaaa accacgggca caaacagcca tatcggttcg
     1261 acagcggtaa ggcttgccgg caatgaaagg ggtgcaaaca acggacagaa tgttaccttt
     1321 gaaaacaacg gcactttggt attggatcaa aacatcaacc aaggcgcggg cggtctgttt
     1381 ttcaaaggcg attacacagt caaaggtgca aataatgaca tcacttggct aggtgcgggg
     1441 attgatgttg ccgacagcaa aaaagtcgtt tggcaagtca aaaatccgaa tggcgacaga
     1501 ttggcaaaaa tcggcaaagg cacattagaa ataaacggca caggcgttaa ccaagggcaa
     1561 ttaaaagtcg gcgacggtac ggttattctg aatcaacaag ccgatgccga caaaaaagtc
     1621 caggctttct cccaagtcgg cattgtgagc ggacgcggta cattggtatt aaatagttca
     1681 aatcagatta atcccgataa cttatatttc ggtttccgtg gcggtcgttt ggatgccaat
     1741 ggcaatgact tgacttttga acacatccgc aacgtggatg aaggcgcgcg cattgtcaac
     1801 cacaacacaa gccacgcctc cacaatcacg ctaacgggta aatctttaat taccaatccg
     1861 aacagccttt cggtacatag catacaaaat gactatgacg aagatgatta tagttattac
     1921 taccgaccaa ggcgcccgat tccgcaaggc aaagaccttt attacaaaaa ctatcgttat
     1981 tacgccctaa aatccggcgg cagcgtgaac gcaccgatgc ccgaaaacgg acaaacggaa
     2041 aataacgact ggattttaat gggcagcaca caagaagaag ccaagaaaaa cgcgatgaac
     2101 cacaaaaaca atcagcgtat tagcagtttt agcggtttct ttggcgaaga aaacggaaaa
     2161 ggacataacg gcgcattaaa ccttaatttc aatggcaaaa gcgcgcaaaa ccgtttcttg
     2221 ttaacaggcg gtgccaattt aaacggaaaa ataagcgtaa cccaaggcaa tgtcttgtta
     2281 tcaggtcgtc caacaccaca cgcaagagat tttgtgaaca aatcttcagc ccaaaaagac
     2341 gcacatttct ccaaaaacaa tgaagtcgta tttgaagacg actggataaa ccgcacattc
     2401 aaagccacag aaattacggt taatcaatcc gcatcattct cttccggcag aaatgtatcc
     2461 aacattaccg ccaacatcac cgcaacagac aacgccaaag taaatttggg ttacaaaaac
     2521 ggcgatgagg tttgcgtgcg ctcggactat accggttatg ttacctgcaa cacaggcaac
     2581 ttatccgata aggctttaaa cagctttgga gcgacacaga ttaacggcaa tgtgaatctg
     2641 aaccagaatg cggcattggt cttgggcaag gctgcattat gggggcaaat tcaagggcaa
     2701 ggaaacagcc gtgtcagcct aaaccaacat agcaaatggc atttgactgg cgacagccaa
     2761 gtacacaatc tgtcattggc ggatagccat attcatttga acaatgcctc cgatgcgcaa
     2821 agtgcaaatc aataccacac gctcaaaatc aatcatttat ccggtaacgg gcattttcat
     2881 tatctgacgc acttggcgga aaatcttggg gataaagtgc ttgtgaagga atccgcatcc
     2941 ggtcattatc agctccatgt tcaagataaa acaggcgaac ccaatcagga agggctgaat
     3001 ctctttgatg catcatccgt acgagaccgt tctcaccttt ccgtttcctt ggcgaatcat
     3061 cacgtcgatt taggcgcatt gcgttataca atcaaaacag aaaatggcat tacccgattg
     3121 tacaatcctt atgccgagaa ccgccgccga gtcaaaccgg ccccgtctcc tgccacaaac
     3181 acggcttctc aagcacaaaa ggcaacacaa acggacggtg cacaaattgc caagcctcaa
     3241 aatatcgtcg tcgcaccgcc tagcccgcag gcaaatcaag ccgaagaagc aaaacgccag
     3301 caagcaaaag cggagcaagt gaagcgtcag caagcagaag cagagcgtaa atcggcagag
     3361 ttagcaaaac aaaaagcaga agccgaacgc gaagcaagag agctggcgac ccgccaaaaa
     3421 gcagaacaag aacgcagcag cgccgaactt gcacgtcgac atgaaaaaga acgcgaagcg
     3481 gcagagttgt cggcaaaaca aaaagtcgaa gccgaacgtg aggctcaagc attggcggta
     3541 cgccgaaaag cagaagccga agaggcaaaa cgccaagctg cagagcttgc acgccggcat
     3601 gaaaaagaac gcgaagcggc agagttgtcg gcaaaacaaa gggtcgggga agaggaacgc
     3661 cgtcaaacag cccaatccca gccgcaacgc cgcaaacgcc gtgccgcacc gcaggattat
     3721 atggcagctt cccaagaccg tcctaaacgc cgcggacacc gttcggtaca gcaaaacaat
     3781 gtggaaattg cccaagccca agcagaactt gcgcgccgac agcaggaaga acgcaaagcg
     3841 gcagagttgt tggcaaaaca aagggcagaa gccgaacgtg aagctcaagc attggctgcg
     3901 cgccgaaaag cagaagccga agaggcaaaa cgccaagctg ccgaacttgc acatcgccag
     3961 gaagcagaac gcaaagcggc agaattgtcg gcaaaccaaa aagccgcagc tgaagcccaa
     4021 gcattggcgg cacgccaaca aaaagccctt gcgcgtcaac aggaagaagc acgaaaagct
     4081 gcagagttag ccgtcaaaca aaaagcggaa accgaacgca aaaccgccga acttgccaag
     4141 caaagggcag cagccgaagc agcaaaacgc cagcaagaag ctcgccagac cgccgaactt
     4201 gcacgtcgtc aagaagcgga acgccaagca gcagagttgt cggcaaaaca aaaagccgaa
     4261 acagatcgag aagcggcaga atcggcgaaa cgaaaagctg aggaagaaga gcaccgtcaa
     4321 gcagcccaat cccagccgca acgccgaaaa cgccgtgccg cacctcagga ttatatggca
     4381 gcttcccaaa atcgtccgaa acgtcgcgga cgcagatcta ctctgccggc accgccctcg
     4441 ccatcatttg attcgtcagc ttacgcagca cccagggcct tgcataatcc ggactggtat
     4501 gaaaatgact atgaagaaat ccccttggat gcgttggaag atgaaaatgt atctgaatca
     4561 gttgacacat cagacaaaca gcctcaagac aatacggaac ttcatgaaaa atatgaaaat
     4621 gattatgaag aaatcccctt ggatgcgttg gaagatgaag atgtatccga atcggttgac
     4681 acatcagaca aacagcctca agacaatacg gaacttcatg aaaaagttga gacagtgagt
     4741 ttgcaaccaa gagccgcgca gccgcgagcc caagccgcca ctcagccgca agcccaagcc
     4801 gccgcgcaag ccgatgcagt cagcaccaat accaactcgg ctttatctga cgcaatggca
     4861 agcacgcaat ctatcttgtt ggatacaggt gcttcattaa cacggcacat tgcacaaaaa
     4921 tcacgcgctg atgccgaaaa aaacagtgtt tggatgtcga acaccggtta tggtcgtgat
     4981 tatgcttccg cacaatatcg ccggtttagt tcgaaacgca cgcaaacaca aatcggcatt
     5041 gaccgcagct tgtccgaaaa tatgcagata ggcggcgtat tgacttactc tgacagtcag
     5101 catacttttg atcaggcgag cggcaaaaat acttttgtgc aagccaacct ttatggtaag
     5161 tattatttaa atgatgcttg gtatgtggcc ggcgatattg gtgcaggcag cttgagaagc
     5221 cggttacaaa cgcagcaaaa agcaaacttt aaccgagcaa gcatccaaac cggccttact
     5281 ttgggcaata cgctgaaaat caatcaattc gagattgtcc ccagtgcggg gatccgttac
     5341 agccgcctgt catctgcaga ttacaagttg ggtaacgaca gtgttaaagt aagttctatg
     5401 tcagtgaaaa cactaacggc cggactggat tttgcttatc ggtttaaagt cggcaacctt
     5461 accgtaaaac ccttgttatc tgcagcttac tttgccaatt atggcaaagg cggcgtgaat
     5521 gtgggcggta attccttcgt ctataaagca gataatcaac agcaatattc agcaggtgcc
     5581 gcgttactgt accgtaatgt tacattaaac gtaaatggca gtattacaaa aggaaaacaa
     5641 ttggaaaaac aaaaatccgg acaaattaaa atacagattc gtttctaaaa tactaaattc
     5701 atagcaaaat aaaatgccgt ctgaactcag gcttcagacg gcatttttat agtggattaa
     5761 ca
//
LOCUS       DAXHNE010000125         5757 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.125, whole genome shotgun sequence.
ACCESSION   DAXHNE010000125 DAXHNE010000000
VERSION     DAXHNE010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.125"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            118..1293
                     /locus_tag="ACFM4Z_001664"
     CDS             118..1293
                     /locus_tag="ACFM4Z_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFC6021667.1"
                     /translation="MLNTFLSPWPCFTQEEADAVSKVLLSNKVNYWTGNECREFEKEF
                     AAFAGTRYAVALANGTLALDVALKAMGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAALTPTTKAVIVVHLAGMPAEMDGIMALAKEHNLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYNHEHAPGFRWLHESFGTNWRMMEMQAVIGRIQLKRLPEWTARRRENAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVNELNARKVPCYQGSCSEV
                     YLEKAFDNTPWRPKERLKNAVELGDTSLMFLVHPTLTNDEIAFCKENIEAVLAEATR"
     gene            1341..3251
                     /gene="pglD"
                     /locus_tag="ACFM4Z_001665"
     CDS             1341..3251
                     /gene="pglD"
                     /locus_tag="ACFM4Z_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HFC6021668.1"
                     /translation="MTLETLLALPRNIKKICFLIHDFLMIFTAFWFTQSLKADYSNEW
                     FDPANWQSFLLTALLTIGLFVRMGLYRAVTRFVSFHVLTTAFAGSLVSAVLFFLNTLI
                     FEEKLRLALPVVYFLLLLVSVTGSRMVIRGLLSDHQKKHMTPVIIYGAGRSGRQLLEA
                     VKQIREYSAVAFVDDNPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPSATQEQ
                     RRRIISKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVRTFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPITLTHPEITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTANETMLPWHELSALLDRIRAACDRYDQQAIRTLLINAPTGFAPSDGICDL
                     LWVRETHRKNAV"
     gene            3307..4599
                     /locus_tag="ACFM4Z_001666"
     CDS             3307..4599
                     /locus_tag="ACFM4Z_001666"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFC6021669.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAEEHAVENIGNYSNPQGDAALIDALTTFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFNLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(4651..5487)
                     /locus_tag="ACFM4Z_001667"
     CDS             complement(4651..5487)
                     /locus_tag="ACFM4Z_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFC6021670.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKRGISGAYDGALALAANKEMVSRTHVAEFLIRVGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKKLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGLLASAGSDFHRLNDFSGGILGACPELPENCKPVWTHFS
                     RH"
BASE COUNT         1368 a         1780 c         1456 g         1153 t
ORIGIN      
        1 ccccgtacag accgattttg acaacacctg cggcgcgcgc ccgattcttc ggaacacgcc
       61 ccccttcaga cggcataggg tcggaaatgc cgtctgaaaa ccgacggaca aaccatcatg
      121 ctgaacactt tcctttcccc gtggccctgc ttcacccaag aagaagccga tgccgtttcc
      181 aaagtcctgc tgtccaacaa agtcaactac tggacgggca acgaatgccg cgaatttgaa
      241 aaagaatttg ccgcctttgc cggcacgcgg tacgccgtcg cccttgccaa cggcacgctg
      301 gcactcgatg tcgcgctcaa agcaatgggc ataggcgcgg gcgacgatgt gattgttacc
      361 tcgcgcacct tcctcgcttc cgcgtcctgc attgtgaacg cgggcgcaaa ccccgtgttt
      421 gccgatgtgg atttgaacag ccaaaacatc agcgcggaaa ccgtcaaagc cgcgctgaca
      481 ccgactacca aagccgtcat cgtcgtccac ctcgccggta tgcccgccga aatggacggc
      541 attatggctt tggcaaaaga acataatctt tgggtaatcg aagactgcgc ccaagcgcac
      601 ggcgcaaaat acaaaggcaa atccgtcggc tctatcggac acgtcggcgc gtggtcgttc
      661 tgccaagaca aaatcatgac caccggcggc gaaggcggta tggttacgac caacgacaaa
      721 accctgtggg aaaaaatgtg gtcgtacaaa gaccacggca aaagctacga tgccgtgtac
      781 aaccacgaac acgcgcccgg tttccgctgg ctgcacgaaa gtttcggcac aaactggcgt
      841 atgatggaaa tgcaggcggt catcggacgc atccagctca aacgcctgcc cgaatggacg
      901 gcgcgccgcc gagaaaacgc cgccaagctg gcggaaagcc taggcaaatt caaaagcatc
      961 cgcttggttg aagtcgccga ctacatcgga cacgcgcaat ataagttcta cgccttcgtc
     1021 aaacccgaac acctcaaaga cggctggacg cgcgaccgca tcgtgaacga actgaacgcg
     1081 cgcaaagtcc cctgctatca aggtagctgc tccgaagtgt atttagaaaa agccttcgac
     1141 aacacgccgt ggcgtccgaa agagcgtttg aaaaatgctg tcgagttggg cgacaccagc
     1201 ctgatgtttt tggtgcaccc gacgctgacc aacgacgaaa tcgcgttctg caaagaaaac
     1261 atcgaagccg tattggcaga agccacacga taacccttca gacggcatat gccgcctgaa
     1321 agcacatacc gccccacgat atgactctgg aaaccctgct tgccctaccg cgcaacatca
     1381 agaaaatctg tttcctcata cacgattttc tgatgatttt caccgccttc tggtttaccc
     1441 aaagcctgaa ggccgactac tcgaacgaat ggttcgaccc tgccaactgg cagtctttcc
     1501 tattgaccgc gctgttgacc atcggcctgt tcgtcagaat ggggctctac cgtgccgtta
     1561 cacgctttgt cagtttccac gtcctgacca ccgcctttgc cggcagcctc gtctccgccg
     1621 tgttgttttt cctcaatacg ctgatatttg aagaaaaatt gcgcctcgcc ctgcccgtag
     1681 tctactttct gctgctgctt gtctccgtaa ccggatcccg catggtcata cgcggccttt
     1741 tatccgacca ccaaaaaaaa catatgaccc ccgtcatcat ttacggtgcg ggacgatcgg
     1801 gcagacaact gctcgaggcc gtcaaacaga tacgcgaata ttccgcggtc gcctttgtgg
     1861 acgacaaccc caagctttgg cacaccgtca tctacgacct tgccgtttac cagcccgatg
     1921 ccatcgcctt cctcatcgaa cgttacggcg tggaaaaaat cctgctcgcc attcccagcg
     1981 cgactcagga acaacgccgc cgaatcatca gcaaactgga agcctatccg tgcgaagtgt
     2041 tgaccattcc cggaatgaaa gacctgatgg acgggaaaat cagcatcggc acgctcaaaa
     2101 aaatctccgt gtccgacctg ctcgggcgcg attccgtcgc acccgacgac cgtctgatga
     2161 gtgccgacat cgaaggcaaa accgtcatgg taaccggtgc gggcggctcc atcggttcgg
     2221 aactctgccg ccagattatc cgccgccgcc ccgaaaaact gctgctgttc gagttatccg
     2281 aattcgccct gtacgccatc gaaaaagaat tgcgcgaaac ctgcatccaa aaacgtctcg
     2341 ataccgaaat cctgcccttt ctcggctcgg tgcaaaaccg cacgctgctc gaacacgtca
     2401 tgaccgcctt ttccgttgcg accgtctatc acgccgctgc ctacaaacac gtccccatgg
     2461 tcgaatttaa caccgtcgaa ggcatacgca acaacatctt cggcacactc gagtgcgcgc
     2521 ttgccgccac ggcatcgggc gtaagaacct tcgtcctcat ctccaccgac aaagcagtcc
     2581 gccccaccaa caccatgggt gccagcaaac gcatggcgga actctgcctt caggcgctcg
     2641 ccgccgaacc cggacaaaaa acccgcttca gcatggtacg tttcggcaat gttttaggtt
     2701 cgtccggctc cgttgtcccg ctgtttgaaa aacagattgc cgaaggcggc ccgattaccc
     2761 tgacccaccc cgaaatcaca cgttatttca tgaccatacc cgaagccgcc caactcgtca
     2821 tacaggcagg cgcgatgggt acgggcggcg acgtattcgt cctcgacatg ggcgaatccg
     2881 tcaaaatcat cgaccttgcc cgccaaatga ttaccctaag cggcctcaaa cccaaaacac
     2941 ccgaacaacc cgacggcgac atcgaaatcc tcattaccgg actgcgtccc ggagaaaaac
     3001 tctacgaaga gctgctcatc ggcgacaacg tccgcaaaac cggccatccg cgcatcatga
     3061 ccgccaacga gaccatgctg ccgtggcacg agctctccgc cctactcgac cgcatccgtg
     3121 cggcctgcga ccgttacgac cagcaggcaa tccgcaccct gctcatcaac gccccgaccg
     3181 gctttgcccc gagcgacggt atctgcgacc tgctttgggt acgagaaaca cacagaaaaa
     3241 atgccgtctg aaccttcaga cggcataacg tacaaaccaa cctaccttac acacacggag
     3301 tttgacatgc agttttcagc attcggcgaa aaattcacgc aacacagcgg catcctccaa
     3361 ctgatggacg acctcggcga cgcgctcaaa agcgacaagc ccgtcaacat gctcggcggc
     3421 ggcaacccgg cgcgcattcc ggaaatcgat cgggcattcg ccgacatatt ctccaaactg
     3481 gcggaggaac acgccgtcga aaacatcggc aactactcca atccccaagg cgatgccgcg
     3541 ctgattgacg cgctgaccac cttcctcaac cgcgaatacg gctggaatct gaccgccgac
     3601 aatatcgcgc tgaccaacgg ttcgcaaaac gcgtttttct atttattcaa cctcttcggc
     3661 ggcaaattca acctttcaga cggcacatcc gcagaaaaag ccattttgtt gccgctcgcg
     3721 cccgaataca tcggctatgc cgacgtgcat gtcgaagggc agcacttcgt ttccgtcaag
     3781 cccaaaatcg aaaacgtcga acacgaaggc gaagcaggct tcttcaaata ccgcgtggac
     3841 tttgacgcac tggaaaacct acccgaactc aaagcgggca aaatcggcgc gatttgctgt
     3901 tcgcgcccga ccaacccgac cggcaatgtg ttgaccgacg gcgaaatggc gcgtttggac
     3961 gctttggcac gtgaacacgg gattccgctg attatcgaca acgcctacgg aatgccgttc
     4021 cccaacatca tttacagcga cgtaacgctg aattggcacg aaaacatcat cctctgcttc
     4081 agcctttcca aagtcggcct gccgggcgtt cgcaccggca tcatcgtcgc cgcgccggaa
     4141 gtggtcaaag ccgtcagcag cctgaacgcg attgtgaacc ttgcccccac gcgcttcggc
     4201 gcggccatcg ccgccccgct actggaaagc ggcgagatga aacggcttgc cgaccaagtc
     4261 atccggccgt tttaccgcaa tcaggcgcaa accgccgtct cgctgctcaa gcgcgagctg
     4321 ggcgcgtacc cgatgaaaat ccacaaaccc gaaggcgcga ttttcctgtg gctctggttt
     4381 gaaaacctgc ccgtttcttc gcaaaccctg tacgaaatgc tcaaagccga aggtacgctg
     4441 attattccgg gcgaacattt cttcgtcggc atcgacacgc aggattaccc gcacgcgggc
     4501 gagtgcatcc gcatgagcat cgcgcaggac gctcaaacgc tggaaaaagg cattgccgcc
     4561 atcggcaaaa ccgtccgaaa actgtacgac aacgtttaaa ccgcaaaaaa tgccgtctga
     4621 aaagttttca gacggcattt ttatccgcat tcaatgtcgg gaaaagtgcg tccacaccgg
     4681 tttgcagttt tccggcaatt cgggacacgc gccgagaatg ccgccgctga agtcgtttaa
     4741 acggtggaag tcgctgcccg cgcttgccaa caatccgaag cgttctgcca aaagcgcgta
     4801 gttgaggcgg tcgtttttgc aacagtttcc gctgtggact tcgatacccg caccgccgag
     4861 ttttttaaat tcttcaaaca gattgcgctt ggcggtggcg gacaaatcat aacgcatcgg
     4921 atgggcgatg accgccaacc cgcccgcgcc gttgacggcg gagacgcagt cttccagcgt
     4981 cgcccattcg tggcggacgg cgcaggattt gccgtcgccc aagtatttgg tgaacgcctg
     5041 ctgcttgttt ttgacgtgtc ccacccgaat gaggaactcg gcgacgtgtg tgcggctgac
     5101 catttctttg tttgccgcca gcgcgagcgc gccgtcgtat gcgcccgaaa tgccgcgttt
     5161 ttccagcttg gcggcgatgg cttcgaggcg tttcagacgg ccttgccgca cttgcgccaa
     5221 caggttttgc aggttttcgt cttgttcgtc aaagtctaag ccgacaacgt gtatggtacg
     5281 cccgcgccac gttacggaga tttccacgcc gttaatcagg cgcaaaccga gcctgtcggc
     5341 ttcggcacgc gcttcggcga tgccgccggt gtggtcgtgg tcggtcaacg ccagcagcgt
     5401 gcagccgttt tgatgcgcga ggcgcacaac ttcggcgggg gagagcatac cgtcggaaac
     5461 ggtggaatgg cagtgcaggt ctatcataag gcgttatgtg cgtggtaaat ggtgggcagg
     5521 gtgtttcaat attgtcgggc ggtgtggcgg cggtgatttc aaaaaacagg tgtatggcaa
     5581 atgcaaagga aaagccgcta tgcggtcatt cccgcgcagg cgggaatcca gactttgatt
     5641 tattggaaat gtttaaggtt aaccgctatt tcaaacttcc ggattcctgc tttcgaggga
     5701 atggcgatat gaccgttttc aattttaatc tactataaaa gactgtctga aaacgtg
//
LOCUS       DAXHNE010000126         5690 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.126, whole genome shotgun sequence.
ACCESSION   DAXHNE010000126 DAXHNE010000000
VERSION     DAXHNE010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.126"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            97..813
                     /locus_tag="ACFM4Z_001668"
     CDS             97..813
                     /locus_tag="ACFM4Z_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFC6021671.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILAGLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            962..1864
                     /locus_tag="ACFM4Z_001669"
     CDS             962..1864
                     /locus_tag="ACFM4Z_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HFC6021672.1"
                     /translation="MDSLMTLLSVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFAFGKLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASADGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIALLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIRGAGGLEAVP
                     VAVSFGVVVNIAAPFLMVVFSALG"
     gene            1907..2680
                     /locus_tag="ACFM4Z_001670"
     CDS             1907..2680
                     /locus_tag="ACFM4Z_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFC6021673.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EEVGSLKRHHGGASSGGNLPEGLPADRQTRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAVVSELVRRRGSLRVITNNIHAASIASARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2792..4480
                     /locus_tag="ACFM4Z_001671"
     CDS             2792..4480
                     /locus_tag="ACFM4Z_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFC6021674.1"
                     /translation="MLNKDQFADNHFIRTIIEEDLESGKHTAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMHRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFEAGRTQSRRAAFHPNHEEMGEREVPISQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4573..5529
                     /gene="gshB"
                     /locus_tag="ACFM4Z_001672"
     CDS             4573..5529
                     /gene="gshB"
                     /locus_tag="ACFM4Z_001672"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFC6021675.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWQLFHTLSGELSV
                     NGGLVTAQASAFEFLGAKNDDDHEWFKAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQAMRDFNEKLAILNFSRFIAPMLVTTRSADVRVFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMQLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELGGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
BASE COUNT         1327 a         1540 c         1582 g         1241 t
ORIGIN      
        1 atttgtgcaa tgctttaatt cctctgacac gatttgaaac acagccgcct taaaacatct
       61 taaaaaattt gaaagcgtaa aaatacggaa aacatcatga actttgaaaa cgacgacatt
      121 atccatgcgc cgaccacgtc ttccctgatt ctcgaagagc ggcacgattc ggagctgttc
      181 cgtgtttacg ccctgatttt ggacggcatt accgatcagg tgctgctgcc cggcaaaaag
      241 ctgaccgaat ccgaactttg ccgtcagatg gtgtgttcgc gcaacaccgt ccgcggcgcg
      301 ctgtcgcttt tggcgcacga caagattgtc gatttgcaac ccaacagggg cgcgttcgtc
      361 cacgttcccg atttgaaaga aatgcaggat gtgttcaatg cgcgcatcga aatggagacg
      421 atgattttga atatcctcgc cggcctgccg gatttggaaa cgcgcctcaa gccgctttat
      481 gcgatgataa ggcgcgaaga agaggcttcc ggcaggggcg accgcgtcgg ctggaaccgc
      541 ctgtccaatg ccttccacgt cgaactggcg cgcctggtgg gcaacgatgt gctgttcgac
      601 attatgaaca cgctgtgcgc gcggtcttcc ctgattgtcg ccgtggcggg cgtgcatcgc
      661 gaggaaaaac acgccatcaa tacgcatacg cattccgaac accgcgaaat cctcgacctg
      721 ctgctggcgg gcaggcgcaa ccgcgtggtc aaaatcctgc gccgccattt gggcaactgt
      781 atggaacgct tggaaaagac tttggaagat tgaatgccgg agcggataag ccgcctgaac
      841 cttcaggcgg ttttttaatg gcgaacctga tgccgtctga aatatggatg cgggtatctg
      901 caattttcag acggcaattt ttaaaccgca catatcatgc ggcaataaag gagggcagga
      961 gatggacagc ctgatgacat tgctttcggt attgataccg atgttcgccg gattttttat
     1021 ccgtgttccc aagccttacc tgcccgcttt ggacaaggtg ctgtcggttt tggtgtatgc
     1081 cgtgctgctg ctgatcggcg tatcgttgtc gcgcgtggag gatttgggtt cgcggttgga
     1141 cgatatggcg ttgacggttc tgtggctgtt tgtttgtacg gtcggggcga acctgcttgc
     1201 tttggcagtg ttgggaaagt tattcccgtg gcggataaag gggaaaggga agggcgtttc
     1261 ggtcggcgtg tcgggcagtg tggggcagct cggatgcgtg ctgctcggat ttgcattcgg
     1321 caaactgatg cgcgatattt ggatgccgtc tgaaagcgcg ggcatgtatt gtctgatgct
     1381 gctggtgttc ctcatcggcg tacagctcaa aagcagcggc gtatcgttgc ggcaggtttt
     1441 ggtcaaccgc aggggtattc ggttgtcggt ctggtttatg ctttcatctc tttcgggcgg
     1501 gctgctgttt gccgcatcgg cagacggcgt gtcgtggacg aaaggtttgg cgatggcttc
     1561 cggcttcggt tggtattccc tctcgggttt ggtgatgacc gaggcttacg gcgcggtatg
     1621 gggcagtatc gcgcttttga acgatttggc acgagagctg ttcgcgctgg catttattcc
     1681 gctgctgatg aagcgttttc ccgatgcggc agtgggggtc ggcggcgcga ccagtatgga
     1741 tttcacattg cccgtgattc ggggtgcggg cggcttggaa gccgtaccgg tagcggtcag
     1801 cttcggcgtg gtggtcaata tcgccgctcc gtttctgatg gtggtgtttt ccgctttggg
     1861 ctgaacgcgg taaaatcggc atcccgatgc aaggaggcag aaaacgatga aaccgaaaat
     1921 ccaaaggcac ggagagattt taagccttgt ccgccggcat cagtttatgt cggtggacga
     1981 gcttgccgcc gcattggacg ttaccccgca gacgatacgc cgcgacatcc gcgagttgga
     2041 ggaggtcggc agcctgaaac gccatcacgg cggcgcatct tcgggcggaa acttgccgga
     2101 gggcctgccc gccgaccgcc aaacccggtg tcaaaacgaa aaaaacgcca ttgcccggct
     2161 gattgcggaa cacatccccg acggttcgtc gctgtttgtc agtatcggca cgacgatgga
     2221 ggccgtggtg tcggagctgg tcaggcggcg cggcagcctg agggtgatta ccaacaatat
     2281 ccacgccgcc tcgattgctt cggcgcggac ggattatacg gtcatcatca cttccggagt
     2341 cgtccgccct ttggacggcg gtattaccgg cgtggcaacc gtcgatttta tcaaccagtt
     2401 caaagtcgat tatgccgtga tgagtacgca cggcgtggaa agcgacggtt cgcttttgga
     2461 cgacgactac aaagaagtca gcgtgatgca ggcgatgatt gccaatgcgc gcgtccgctt
     2521 cctcggcgtg gaccacagta aattccgcag caacgcgctg gtcaggctcg gcgacattac
     2581 ggcgtttgac aaagtattta ccgaccggct gcccgatacc gcgatgcaga agatgctgaa
     2641 agaggcgggg gtggaatgct tgattgccga tgccgtctga acgctatgtc aaagcgcgca
     2701 agtcgggtac aataaacaca tcatcaaacc gcttcagacg gcataaggaa ccgccccaat
     2761 gccgtctgaa gccatctgtt caaagaaaac tatgctcaat aaagaccaat tcgcggacaa
     2821 ccactttatc cgcaccatca tcgaagaaga cctcgaaagc ggcaaacata cagctgtcca
     2881 aacccgtttt ccgccggagc ccaacggcta cctgcacatc ggacacgcca aatccatctg
     2941 cctgaacttc ggtttggctt atatttatga cggtttgtgc aacctgcgtt ttgacgatac
     3001 caaccccgaa aaagaaaacg acgaatacgt caacgccatc aaagaagacg ttgagtggtt
     3061 gggcttccat tgggcgggcg agccgcgttt cgcttccaac tatttcgacc agctttatga
     3121 ctacgccgtc ggtttggtta aagacggcaa agcgtatgtc gatgatttga cgccagaaga
     3181 aatgcgcgaa taccgcggta cgctgaccga agcaggcaaa aacagccctt atcgcgaccg
     3241 cagtatcgaa gaaaacctcg acctgttcac gcgcatgaaa aacggtgagt tccccgacgg
     3301 cagcaaaacc ctgcgcctga aaatcgacat ggcatccggc aacatcaata tgcgcgatcc
     3361 ggtcatctac cgcatccgcc gcgcccatca ccacaacacc ggcgacaaat ggtgcatcta
     3421 tccgatgtac gactacacgc attgtatttc cgatgccatc gaaggcatca cgcattcctt
     3481 gtgtacgctc gaatttgaag cccatcgtcc gctgtatgac tgggtgttgg acaacatccc
     3541 cgcgccgcac gccacacgtc cgcgccaata cgagttttcc cgtttggagc ttttgtacac
     3601 cattacctcc aaacggaaat tgaatcagtt ggttgtggaa aaacacgttt ccggctggga
     3661 cgatccgcgt atgccgacca tttccggtat gcaccgacgc ggctacacgc ccgaaggcct
     3721 gcgtctgttt gccaaacgcg ccggtatttc caaatctgaa aacatcgtcg atatgagcgt
     3781 gttggaaggc gcgattcgcg aagagttgga aaactccgcc ccgcgcctga tggcggtgtt
     3841 gaacccgctc aaagtaaccc tgaccaactt tgaagccggc agaacccaaa gccgccgtgc
     3901 cgcgttccat ccgaaccacg aggaaatggg cgagcgcgaa gtacctattt cacaaaccat
     3961 ctatattgaa gccgacgact ttgccgaaaa tccgcccaaa ggcttcaaac gcctgattcc
     4021 cggcggcgaa gtacgcttgc gccacggcta tgtcatcaag tgcgatgaag tcgtcaaaga
     4081 cgaagcaggc aatgtggttg aactcaaatg cagcatcgac cacgacacct tgggcaaaaa
     4141 tcccgaaggc cgcaaagtca aaggcgtaat tcactgggtg tctgccgaac acgcagccga
     4201 aatcaaagtc cgtctgtacg accgcctctt taccgtcgag cgtcccgatg ccgtgcgcgg
     4261 cgaagacggc gaatacctgc cgttcaccga tttcctcaat ccggaatccg tcaaggaaat
     4321 caccgcctac gccgaacctg ccgccaaaga tttgccggca gaaagccgtt ggcagttcga
     4381 gcgtatcggc tacttcgtaa ccgaccgtaa agaccacggc aaagacacgc cggtgtttaa
     4441 ccgcacggtc acgctgaaag attcttggca gcctaagtaa aaccccatcc ttgccgtctg
     4501 aatattgttc ggacggcatt tcccctttgc ccgcgaaatg cggcacattc ggcacaccgg
     4561 aaaggaaata tgatgaaagt cctctttatc gccgacccga tggcaagttt caaaacctac
     4621 aaagacacca cctacgccat gatgcgcgag atggcaaaac gcggctggca actgtttcac
     4681 acattgagcg gggaattgtc tgtaaacggc ggtctggtaa cggcacaggc atcggcattt
     4741 gaatttttgg gtgcaaaaaa cgatgatgac catgaatggt ttaaagcggc ggacaaagtt
     4801 cagacggcat tgaaagcatt tgatgccgtg attatgcgta ccgatccgcc gttcgatatg
     4861 caatacctct acgccaccca attactgacg ctggcggaac agcagggcgc gaaagtcttt
     4921 aacagcggac aggcgatgcg tgatttcaat gaaaaactgg cgattttgaa cttcagccgc
     4981 tttatcgcgc ccatgctggt aacgacccgt tccgccgatg tccgcgtatt tttgaaagaa
     5041 cacggcgaca tcatcattaa accgctcgac ggcatgggcg gtatgggcat cttccgcctg
     5101 accgaaaaag accccaacat cggcagcatc ctcgaaaccc tgatgcagct tgattcccgc
     5161 accattatgg cgcaacgcta cattcccgaa atcgtacacg gtgacaaacg catcctgatt
     5221 atcggcggcg aagtcgtccc ctatgctttg gcgcgtatcc cgcaaaacgg cgaaacacgc
     5281 ggcaaccttg cggcaggcgg gcgcggcgtg gcgcaggaat tgggcggacg cgaccgcgaa
     5341 attgccgaaa cgctcgcccc cgaactcaaa cggcgcggca tcctgctggc cggtttggac
     5401 gttatcggca gcaacctgac cgaagtcaac gtaaccagcc cgaccggatt ccaagaaatc
     5461 atgaaacaaa aaggtttcga tgtggcggca atgtttgccg atgccgttgc cgcgtggtcg
     5521 gtacgttaaa ccgatgtcgt ctgaaaggct tttgcttcac aaccgtttgg tttgttcggg
     5581 caggcagggt tttccctcag tcggcagaga tacgcttaat gccgtacgga cacaccgccg
     5641 ccccgttttc agacggtata tatagtggat taacaaaaat caggacaagg
//
LOCUS       DAXHNE010000127         5666 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.127, whole genome shotgun sequence.
ACCESSION   DAXHNE010000127 DAXHNE010000000
VERSION     DAXHNE010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.127"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..207
                     /locus_tag="ACFM4Z_001673"
     CDS             <1..207
                     /locus_tag="ACFM4Z_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021676.1"
                     /translation="FSELYMFQQRMAHQAPHCVLPERDYPPIRFYRYKQTSFNRKGMG
                     IKSISDTSRAMPSENQSPLSDGIV"
     gene            284..1231
                     /locus_tag="ACFM4Z_001674"
     CDS             284..1231
                     /locus_tag="ACFM4Z_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFC6021677.1"
                     /translation="MNILSKISSFIGKTFSLWVALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMTYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAG
                     SMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLAIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(1383..2507)
                     /gene="rluD"
                     /locus_tag="ACFM4Z_001675"
     CDS             complement(1383..2507)
                     /gene="rluD"
                     /locus_tag="ACFM4Z_001675"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFC6021678.1"
                     /translation="MQNTSFDNESDYSDDSDFVSAPETENCICLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVQQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLAHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPKTGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            2506..3309
                     /locus_tag="ACFM4Z_001676"
     CDS             2506..3309
                     /locus_tag="ACFM4Z_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFC6021679.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(3367..5586)
                     /locus_tag="ACFM4Z_001677"
     CDS             complement(3367..5586)
                     /locus_tag="ACFM4Z_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFC6021680.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNASGIRYLDYLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVADGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1371 a         1787 c         1359 g         1149 t
ORIGIN      
        1 ttctccgagc tttatatgtt tcaacagagg atggcacatc aagcaccgca ctgcgtgttg
       61 cccgaacgag actaccctcc gattagattc tatcgctata aacagacgag tttcaaccga
      121 aaaggaatgg ggataaagtc catttccgac acctctcggg cgatgccgtc tgaaaaccaa
      181 tctccacttt cagacggcat tgtttaggca cggtcatttt gcaaattaag gttaaggcgt
      241 gtatgcttgc cttcccttaa aaacaaacac gcaacgcatt attatgaata tcctcagtaa
      301 aatcagcagc tttatcggaa aaacattttc cctctgggtc gcgctctttg ccgccgccgc
      361 ttttttcgcg cccgacacct tcaaatgggc ggggccttat attccttggc tgttgggcat
      421 tattatgttc ggtatgggtt tgacgctcaa accttccgac ttcgatattt tgttcaaaca
      481 tcccaaagcc gtcatcatcg gcgtaatcgc acaattcgcc attatgccgg caaccgcctg
      541 gctgctgtcc aaactgttga acctgcctgc cgaaatcgcg gtcggcgtga ttttggtcgg
      601 ctgctgcccg ggcggtacgg cttccaatgt gatgacttat ctggcgcgcg gcaatgtggc
      661 tttgtcggtt gccgttacgt ctgtttccac cctgatttcc ccattgctga ctcccgccat
      721 cttcctgatg cttgccggcg aaatgctgga aatccaagcg ggcagtatgt tgatgtccat
      781 cgtcaaaatg gttttgctcc ccattgtttt aggtttgatt gtccataagg ttttgggcag
      841 taaaaccgaa aagctgaccg atgcgctgcc gctggtttcc gttgccgcca tcgtgctgat
      901 tatcggcgcg gttgttgggg caagcaaagg caagattatg gaaagcggcc tgctgatttt
      961 tgcggttgtc gtactccaca acggcatcgg ctacctgctc ggcttctttg ccgccaaatg
     1021 gaccggcctg ccttatgatg cacaaaaaac gctggccatc gaagtcggta tgcaaaactc
     1081 gggcctggcc gccgcgcttg ccgccgcaca ctttgccgct gcgccggtcg ttgccgttcc
     1141 gggcgcattg ttcagcgtgt ggcacaatat ctccggctcg ctgctggcaa cttattgggc
     1201 ggccaaagcc ggtaaacata aaaaacccta aacgcctttc atcataatgc cgtctgaacg
     1261 gggcaaacaa aaccggcggt ttccgcccga ccccttcaga cggcctttgt ttgaaatcgg
     1321 cgggaacgat tgcccggctg ctccgattct gctgcctgcg ttcgccccgc ctttcaagcc
     1381 gcctactccc ttacataaac cacttcgaca tcgtagtcgt cttcgttcca atcgtcatcg
     1441 tcgtccgcgc cgaatttgtc ctgccattcc tcttcattgc tcaaagacga atccaaaccg
     1501 gcttcaagac ggaggacaga caacaaatga tatatgtcgt ccggaatcgg tgcttcaaac
     1561 gaaacggttt cgccggtttt cggatgagtg aaacttaagc ggtaggcgtg caatgcctga
     1621 cgcgcaccca aacttttaac ggcttctttc accgtgtcgc cgcacggatg gcgcgggttg
     1681 ccgtaaaccg ggtcggcggc aagcggatgg ttggcctcac gcatatggac gcggatttgg
     1741 tgcgtcctgc ctgtttcgag cgagcattcg atatagctgt gcgccagata gcgttccaac
     1801 actttgacgt gggtaacggc tggtttgccg ccgaatttga cgactgccat tttcaggcgg
     1861 ttgtgcggat cgcgtccgat ttgggtttcg attttaccgt caaagggaac gatgccgttg
     1921 gcgacggcgc ggtaaatgcg tttgaccgtg cgctcttgaa gctgttgcac gagggaattt
     1981 tgcgccggca gggttttggc aaccaccatc agcccgctgg tttctttgtc caaacggtgt
     2041 acgatgcctg cacgaggtac ttggctcaat tcgggacaat gcgccaagag gccgttgagc
     2101 agcgttcccg tccagttgcc cgccgccgga tgcaccacca gtccggccgg tttgttgacg
     2161 acgatgacgg tatcgtcttc gtaaacaata tccaaagcca taggctctgg aacaaacgcc
     2221 agattttcct cactcggacg gacggttaca caaatttgct cgccgcctat cattttgtct
     2281 ttgggttgcg aaggtttatc gtttacaata accgcgcctt ctttaatcca tgatgtcagg
     2341 cggctgcgcg agtagtcggg cagaagtttc gccaacaccg catccagccg cccgccggca
     2401 agctcgagcg gaacagtcag acaaatacag ttttcggttt ccggggctga cacaaagtct
     2461 gaatcatcgc tataatcgga ttcattatca aaggaagtat tctgcatgaa aaaaattctt
     2521 ttaacggttt cattaggttt ggcgctgagt gcctgtgcca ctcaaggtac ggtagataaa
     2581 gatgcccaga ttacccaaga ctggagtgtg gaaaaactct atgccgaagc ccaggatgag
     2641 ctgaacagca gcaattatac tcgagctgtc aaattatacg aaatcttgga atcgcgcttc
     2701 cccaccagcc gccatgcccg gcagtcgcaa ctggataccg catacgccta ttataaagac
     2761 gatgaaaaag acaaagctct ggcggcaatc gaacgcttcc gccgccttca ccctcaacac
     2821 ccgaatatgg attacgcgct gtatctgcgc ggcttggtgc tgttcaacga agatcagtct
     2881 ttcttgaaca aactggcctc gcaagactgg tccgaccgcg acccgaaagc caaccgcgaa
     2941 gcgtaccagg cgtttgcgga actcgtccaa cgcttcccga acagcaaata cgccgccgat
     3001 gcaaccgcac gcatggtcaa actggtcgat gcactgggcg gcaatgaaat gtcggtggcg
     3061 cgctactaca tgaaacgcgg tgcatacatc gccgccgcca accgcgccca aaaaatcatc
     3121 ggcagctacc agaatacgcg ttatgtcgaa gaatcgctcg ccatattgga acttgcctac
     3181 caaaaactcg gcaaaccaca gcttgccgcc gatacgcgcc gcgtgttgga aaccaacttc
     3241 ccgaaaagcc cgtttttgac gcacgcttgg cagcccgacg atatgccctg gtggcgttac
     3301 tggcattaag cccatcttcg taatgccgtc tgaagccctt tcagacggca ttttatgttt
     3361 caaacctcat tcaaacggtt ttttctgcca atagaattta taccctctca aacgttgggc
     3421 cttcacgccg ccgcgtccca agccgaattg cagtgcgccc gacaaatcgg tacgcagcag
     3481 tttgatgccg tgtgctcgga cacggttctg caccgcttcg gtcggatgtt tgtaggcgtt
     3541 ggcataaccg cttgaagcaa cggcatattc gggcgagacg gcattgagga acacgcccga
     3601 cgaggacgta ttgctgccgt gatgtcccaa caccaaaacc tggctgtaca ggttgcctcc
     3661 atacttgccg accaggcttt cctcaccctt cgtatccaaa tcgcccgtta ccagcagtgc
     3721 cgcaccatca gccacaacac gcaaaacaca acttttccca ttatcatcga tgtttttgcg
     3781 ttcagacggc atcaaaaact cgaaatccac gccgtcccat tgccaacgct gttccgcaca
     3841 atgccgcgcc ccctcataaa attccggctg tccggcataa atcccgccgt tcggtatctt
     3901 gccgactgcc tgaaaaccgc cgtcgtggtc gctgtcgtga tgcgacaaaa ccaaataatc
     3961 caaatacctg attcccgacg cattcaaact cggcacaatc cccatctgcg ccgccgctgc
     4021 cgttccggtg tcaaacaaaa gatgatgatt tgccgtctga accgacaccg acaaaccctg
     4081 ccccgcatcc caaaccgtaa ccgcaacctc attttccggc acgccgggtg aacggtaaaa
     4141 cacaaaccct gccaacagca acaccgccca cggacgcaaa cccaagccgc gcggcagcag
     4201 caacagcaaa gcggcacaca ccgccaacac caacagcggc aaaggtgcgg aggcaacggc
     4261 aaactcgggc gacacatcgg caagccacac caaaaaccgc aaagtatatt ccgccaaaaa
     4321 tgcccccaac tgttgcaaag gcgcaaacgg cacgaccgaa cccagcaacg ccagcggcgt
     4381 caatacccaa gaaaaccacg gaattgccac cgcattgacc aaagggctga ttaaaggcag
     4441 cgaagcaaac agataaccga gcaaaaccag cgacaacacc gaagccgccc actgcccgcg
     4501 caaagcagtt tgccgttttc cctcgtgcaa acgccccgag cacgcccata tcagggccgc
     4561 caccaaaccg aaagacagcc aagtccccac acccaagacc gccgacgggt cgaacagcag
     4621 caccgccgcc aacgcctgcc accacgtcgc ccacgccgac aatcttcccc tgcgccaagc
     4681 ccacgcaaac gccgccaaca tcaaaacgct gcgctgcgtc ggcacggaaa aaccggcaag
     4741 cagcgcgtaa aacagcgcgc ctgcacaccc cgccgccaaa acccacgcgc gcggccgggc
     4801 aggaaggcgc ggggaacagg caagcagccg cttcgccagc cacgcaaaca ttaccgccac
     4861 catcgtaacg tgcaaacccg aaatactgac caaatgcgtc agccccaacg gccggaacgc
     4921 ctgccacaat tcggggcgca atgccgactg ttcgcccacg ctcaacgcgc gcatcagccc
     4981 gatgccgtct gaaagcccgc cgtccgcatc cgcctgctgc caattacggc tgatgcggct
     5041 gcgccaaacc gcaatccccc aaccgcttcc gccatgcagc aaaaccctgt ccgcaccgac
     5101 cgtccccgcg ccgcctatcc cgttggataa tgcccacgcc tcacggttca aaccgcgcag
     5161 gttcaattcg ccgacgacgg ggtgcacacg tgccgttatc cgccatctgc tcccgaccgc
     5221 ccattcgcgc cgtttgtagt ccgacagcag caaatcaaac gtccgaccac cgctgtccac
     5281 agcctttgcc gcaaactgca cacgccgccc gtccgacctc ggcatatccg ccacttccac
     5341 cgtcaaaggc acgcctgaaa cggcttctgc ccgccattgc gaagacagtg ccgcttccgt
     5401 tctgaatacg ccgtaagccg cgcccgccaa cacgcacagc atcagaccgg caaacgcaaa
     5461 acgccttgca aacacagcaa aaaccgcaaa agccgccagc caaaacagcc aatgcggcac
     5521 gaccggcagc gcaaacgaag ccgccgcgcc gacaacccaa cacggcaaaa accaacgacg
     5581 gaacacgtct ccccacccca gatatttcaa aaataaacgg ataataaagc aaatccgaaa
     5641 aaccgtcata acaagatttg aaaatg
//
LOCUS       DAXHNE010000128         5552 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.128, whole genome shotgun sequence.
ACCESSION   DAXHNE010000128 DAXHNE010000000
VERSION     DAXHNE010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.128"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(44..811)
                     /locus_tag="ACFM4Z_001678"
     CDS             complement(44..811)
                     /locus_tag="ACFM4Z_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFC6021681.1"
                     /translation="MSSNKASFFTRLRRLCRLAVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIRV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(899..1726)
                     /gene="mutM"
                     /locus_tag="ACFM4Z_001679"
     CDS             complement(899..1726)
                     /gene="mutM"
                     /locus_tag="ACFM4Z_001679"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFC6021682.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLLIRFQTGVLLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYARLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPRCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(1778..2443)
                     /locus_tag="ACFM4Z_001680"
     CDS             complement(1778..2443)
                     /locus_tag="ACFM4Z_001680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6021683.1"
                     /translation="MDSWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGVPSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRRLADVGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     VAD"
     gene            2622..4598
                     /locus_tag="ACFM4Z_001681"
     CDS             2622..4598
                     /locus_tag="ACFM4Z_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC6021684.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWGELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMCIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            4666..5358
                     /locus_tag="ACFM4Z_001682"
     CDS             4666..5358
                     /locus_tag="ACFM4Z_001682"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFC6021685.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHSLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLKNPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
BASE COUNT         1450 a         1784 c         1200 g         1118 t
ORIGIN      
        1 atccacaaaa gcgaatcata ctgccacatt ccgacaaccg gtttcagaca gcgatatccg
       61 catcgtcggc gacaacggca cggatgcttt cttcgatttt atcttttaaa gcataacggt
      121 cttcgctttc cgccgcatcc gccacgcaaa cgaaatcgac tcttatcgtc aattttttca
      181 tagacacgat gcgccacagg caggtcggca aaccgacatc ggcatatgag ggacgagccg
      241 tcctttttcc cgtttcgtca taataacgca gcgcgaccgc caaaaccttt gcccccgcat
      301 cgatggcgga ttggaacagc gcggctttga acggcaaaag ccccaatccg gaggaagtcc
      361 gcgcttcggg gaaaaaactg acgttttgac cgcgttgcaa ggtttcgcag acggcgcggt
      421 taatcggttc gatgtcgcgc cgcgaattgc ggttgatgaa caccgttccc gcgttctgcc
      481 ccatcttgcc caataccggc cagcttttga tttcctgctt ggcgataaag ctgctcggat
      541 aaaccgcgct catcgcgaaa atatccagcc aggacacgtg gttggcggca accaagacac
      601 cgttcggatg ttcgggtgcg ggtctgccca cctccaatcc gatatccaaa gccgccaaaa
      661 cccccctgcc caactcgatt accgcccgat tgcgcgactc ggggcaaccg ccgtcaatac
      721 cgcgcaggtt tttcccggtt ttgaacagcc agaccgccaa acggcacaag cggcgcagac
      781 gtgtaaaaaa tgaagcttta tttgaagaca tgagcaattc tttcccgata atcggtgttt
      841 gttgttgaaa cgttcggacg gcataccgat aaaatgccgt ctgaaaccgt tttcagtcct
      901 atttctgaca gttcgggcaa taaaacgtgc cgcgctgccc caaagtttct ttcacaacca
      961 aaccgccgca ccgggggcac ggctgattgt gccgcccgta cactgtatat tcctgttgga
     1021 agtagccgct tttgccgtcg ctgtccacaa aatccctcaa ggtactgccg cccgtttcaa
     1081 tggcgcgctg caacaccgct ttgacggttt caaccaaaag cgcgcactct ttctttttca
     1141 ggcggttggc aggacggtgg ggcgaaatgc ccgctctgaa caggctctcg ttggcataaa
     1201 tgttgcccac accgaccacg accgcattgt ccatcagggc aagtttgacc gcgcgcttct
     1261 gcgccttcag ccttgcatac agataatccg cacaaaatgc ctccgacaaa ggctccggcc
     1321 ccagtttttc caacagcgga tgatgttctt cgattccctc ataccaaagt atcgcgccga
     1381 actttctcgg atcgcggtaa cgcatgaccg tgccgtctga aaacacaata tcgacgtgat
     1441 cgtgtctgtc cggcctgccg atacgtccgt ccgacggcgt aaaaatccgc aagctgcccg
     1501 acatccccaa gtgaatcagc agcacgcccg tttgaaagcg gataagcagg tatttcgccc
     1561 tcctgccgca ggacaacacc tgccggccgg acaaaatctc ccccaaatcg ggattaatct
     1621 gccagcgcag cttcaattgg cgcaatacca cggcttccac cgttttccct tcaatatgcg
     1681 gcgcgatgcc gcgcaacgtc gtttccactt ccggcaattc aggcataacc cctcccgaca
     1741 tttcttctga cagatgccgt ctgaaagacg gctgtcccta atccgcaacc cttgccgcac
     1801 ccgccgcaag ggctttgccg cccaaatacc cgtcccacgc gggcaggggc gttacgcccg
     1861 ctgccctttt gaccaaaacc aaaccgtaca ctgcggcaca ctgcggccac caacggtcgc
     1921 ccgccttttc cataaaccgc caaaagcgta tttgcccgag cgacgaaacc ggcggcagat
     1981 acaccataaa tttcccaaat tcaatatcga aaccgacatc cgcaagccgt cttttcaact
     2041 cgggcagcgg cagacaaaac cgtttttccg gcaggcgttc gccgtcaaac caacggctga
     2101 atccccagag cgaatacgga ttgaaacccg tcagcatcaa gcgtccgcac ggtttcaata
     2161 tccggtgcgc ttccgacagg atttgcgaag gaacaccgcc ttcaagcgta tgcggaaaaa
     2221 gcagcatatc cgcagaaaca tccgccaaag ccatattctc cgccgacatc gacatatctc
     2281 gcggcacaca gacaacatct tcagacaggc tcagccacgg accgcccacc tgaaccgcac
     2341 acattcccga aaaacggtat gaatccagat accgcccgaa gaaatcctgt tccaattttg
     2401 caacataccg ccccatcgcc gtatcttcaa accatgaatc catattgcgt ccgtttcaaa
     2461 cagattgcct gccgatattc tattccaaac aggatttctg tcaaaaaaca catcggccgc
     2521 ccatttccga atccgcataa agttcctgta aaacttgacg ctttttcagt caaacagtac
     2581 catcggacga taaaatatgt ttattccgcc gaatataaat catgtccaaa ctcaaaacca
     2641 tcgctctgac cgcatcaggt ctgtccgttt gtccgggttt cctatacgcc caaaacacct
     2701 catcacacca aatcggtttg gcgattatgc gcttaaactc ttcaatactc gacctgcccc
     2761 cgacaaaaca atatttccaa tccggcagcc tgtggggcga gctgcgccaa ggcttccgga
     2821 tgggcgaagt caatcccgaa ctggtacgcc gccacgaaag caaattcatc gcaagccaca
     2881 gctatttcaa cagggtcatc aaccggagta gaccctatat gtaccatatc gccaacgaag
     2941 tcaaaaaacg caatatgccc gccgaagccg ccctgcttcc cttcatcgaa agcgcgttcg
     3001 tcaccaaagc caaatcacac gtcggcgcat caggattatg gcagtttatg cccgctaccg
     3061 gcaggcatta cggcctggaa aaaacaccgg tttacgacgg caggcacgac gtttacgccg
     3121 ccaccgatgc cgcactcaac tatctgcaat acctctatgg actgttcggc gactggccgc
     3181 ttgcctttgc cgcctacaac tggggtgaag gcaacgtcgg acgcgccatc aaccgcgccc
     3241 gcgcccaagg gctcgaaccg acctacgaaa acctgcgtat gcccaacgaa acgcgcaact
     3301 atgtccccaa gctgctcgcc gtgcgcaaca ttattgccac tccccaatct ttcggcatga
     3361 atatcagcga catagacaac aaaccctatt ttcaggcagt cgaaccggat cgtccgctcg
     3421 acaacgaagc catcgcccgg cttgccggca tcacgcaaag cgagctgctc gccctaaacc
     3481 ccgcattcaa cgtccccgcg tttatcccca aaagcaaacg caaactgctg cttcctgtcg
     3541 cgtccgtaca aaccttccaa agcaactacc tcaacgccgc acccgacagc ctgttttcat
     3601 gggaagtcta tacgcctgcc gccaaaacca gcctgtccga catctcgacg gcaaccggca
     3661 tgtgcattgc cgacatcaaa cgcctcaaca acctgaacgg caaccttgtc aacgcaggac
     3721 gcagcatcct tgtcgccaag aacggcaaaa cccttcagac ggcatcggaa tccgtcgttt
     3781 ccatcgacat cgacaatacg cccgacacct accgttccaa tatgccggca ggcacggtga
     3841 acgtcggcat tgcccgaatc cgacccgccg ccgcacagac agcggacatt accgtcgcac
     3901 ctttgccgca gaaaaccgtc cgtacggaac ccgatcccct tgtccgtatt gccgaacctg
     3961 cccttgcgac agccgcagcg caacctcaaa ccgaaaaaca gaccgccatg ccgtctgaaa
     4021 cccaaaccgc aacactcgcg cagatcatcc cccaaaacga catgcaggcg gcagacgaac
     4081 tcatgcagct tgttgcccga aacaacctgc gccgacaggc tgaagaaacc atctccgccg
     4141 tcatcggcac gcctgacaca gttgccgaac acaacatttc ctcatctccg caacataccg
     4201 ttgccgccga cggcaaacgc cgggcacgtt tggaaacgcg cgtagccaaa gctgccgacg
     4261 gcgaagccga aatctccccg ctccatgcca gcatccaccg cgttgtagaa ggcgacaccc
     4321 tgttcaacat tgccaaacgc tacaacgtca gtgtagccga cctgattgtc gccaacaaca
     4381 tcaaaggcaa caccatccaa aaaggacagg tactccgctt ggcgcaggca gcccctgccc
     4441 aaacccgtat tgaaaaagta tcctacaccg cgcgcaaagg cgacaccttc aaaagtatcg
     4501 ccgcgcgctt caatatccat atcgacgaca tccgccggct caatcccaac ctgaacacca
     4561 tcaatccggg acagagggtc aaactgattg gaagctgatt cggatacggc acatgacagg
     4621 actttctcag tcctgtcttt ttcatcccac atttcccata ccatcatgaa acttatcaaa
     4681 tacctgcaat atcaaggcat aggaagccgc aagcagtgcc aatggctgat tgccggcggt
     4741 tatgttttca tcaacggaac ctgcatggac gacaccgatg cagacatcga ttcctcatcc
     4801 gtcgaaacgt tggatattga cggggaagca gtaaccgtcg ttcccgaacc ctatttctac
     4861 atcatgctca acaagcctga agattacgaa acttcgcaca aacccaagca ctaccgcagc
     4921 gtattcagcc tgttccccga caatatgcgg aacatcgata tgcaggcggt cggcaggctg
     4981 gatgcagata cgaccggcgt attgctgatt accaacgacg gcaaactgaa ccacagcctg
     5041 acttcgccga gcagaaaaat tcccaagctg tacgaagtaa cgctcaaaca ccccacagga
     5101 gaaacgctct gcgaaacctt gaaaaacggc gtgctgctcc acgacgaaaa cgaaaccgtt
     5161 tgtgccgccg atgccgtttt gaaaaacccg accaccctgc tgctgaccat taccgaagga
     5221 aaataccacc aagtcaaacg catgatcgcc gccgccggca accgcgtgca acaccttcat
     5281 cgccggcgat tcgcacatct ggaaacagaa aacctcaaac ccggggaatg gaaatttatc
     5341 gaatgtccaa aattctgaaa taacatccaa gaattccatt tatattcatc cacatactca
     5401 ttaaatatat ggtttaaccc aatttaacgc aaaaaatcaa tttacgatat aatccatttg
     5461 tctgagtaac gcctgttcaa gcaggcttat gagtaagacg ttttccccgt aatgtgtttt
     5521 gccatctata cttctcccct tgtatagatg gt
//
LOCUS       DAXHNE010000129         5545 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.129, whole genome shotgun sequence.
ACCESSION   DAXHNE010000129 DAXHNE010000000
VERSION     DAXHNE010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.129"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            52..1455
                     /locus_tag="ACFM4Z_001683"
     CDS             52..1455
                     /locus_tag="ACFM4Z_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6021686.1"
                     /translation="MNNPDLPYRQALECLSQKQYNFTEVRRLLTEAFSAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPPETLIPLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALEWAKTSAKNNNPHGQYLLAQYCRYGTPPDFETA
                     HLLYRKAAAQGLPEAHWQLGLQYRFGQGTKVDTAQAVNHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDEAVHWFQQAAQENDPDAHAALADIYLQGKHLERNHKLALHHAEAAAAERHP
                     EGLRILGDICRYGLGIAPDTEKARHYYRQAAEAGSLSAYQKLISDSALNHPDQYGGIK
                     DSAIRRQRAERLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVEPDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQNAV"
     gene            1577..2293
                     /gene="trmB"
                     /locus_tag="ACFM4Z_001684"
     CDS             1577..2293
                     /gene="trmB"
                     /locus_tag="ACFM4Z_001684"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFC6021687.1"
                     /translation="MTDTAENQTQNNWQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIDENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(2921..3283)
                     /locus_tag="ACFM4Z_001685"
     CDS             complement(2921..3283)
                     /locus_tag="ACFM4Z_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021688.1"
                     /translation="MSQLDFNLELFIKNSNFDDYKIFDIVKEAIETQDEIGSILRTHL
                     FFERILEAWIYAKCNKEDFFNDTQIGFRTKLKIARNLGLFSEVYDIGERLNKIRNTIA
                     HHVLREVNQENLTLYMKV"
     gene            complement(3396..5495)
                     /gene="uvrB"
                     /locus_tag="ACFM4Z_001686"
     CDS             complement(3396..5495)
                     /gene="uvrB"
                     /locus_tag="ACFM4Z_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFC6021689.1"
                     /translation="MPSERRFRPIPTARFVRKRITGKIMEVIQYPNSPFKLHQPFPPA
                     GDQPTAIAGLLEGLSDGLAYQTLLGVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQ
                     LYAEMREFFPENAVEYFVSYYDYYQPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNL
                     MTRNDVIIVATVSAIYGIGDPTEYQQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDF
                     KRGSFRVRGDVIDVYPAESSENALRISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPS
                     SHYVTPRDTVLRACESIKEELRERIEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCK
                     GIENYSRHFSGKKEGEPPPTLMDYLPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLV
                     DYGFRLPSARDNRPLKFHEFEKVMPQTIFVSATPAKYEEEHAGQVVEQVVRPTGLVDP
                     QIIIRPVATQVDDLMSEINDRIEKGERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSD
                     IDTVERVEIIRDLRLGLFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQ
                     TIGRAARNVNGVAILYADKITDSMKAAIDETERRREKQIKFNEEQGIVPQQIKKQVKD
                     IIDGVYHEEDGSKGRLKGKNKVKVGEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAV
                     LRDRIRDIKENLLFGAE"
BASE COUNT         1396 a         1412 c         1382 g         1355 t
ORIGIN      
        1 ctaacttcat aacgaataac gatgattcga caaaacggaa aacgatctga catgaacaat
       61 cccgacttac cctatcggca ggccttagaa tgcctgtctc aaaaacaata taactttacc
      121 gaagtccgcc gactgctgac agaagcgttc tcggcaggtc atcccgccgc cgcattcgag
      181 ttggcaaaac acctgatgga cgcggacagc ccctaccaag accgcgaaca aggtatggaa
      241 atgctccgca tcgccgctga acagggacat ccctacgcgc gttacaatct ggcatatatc
      301 caagaattgg aaggcgcacc cccggaaacc ctgataccgc tttacagacc gttggcagaa
      361 gaaggactgc ccgaagcgca agtccgcctg atgtaccttc tgtacgcgtc ccgacatttt
      421 gaagaagcct tggaatgggc aaaaacaagc gcaaaaaaca acaaccccca cgggcaatac
      481 ctgcttgccc aatactgccg gtacggcaca ccgccggatt ttgaaacggc gcacctgctc
      541 taccgaaaag cggcggcaca aggcttgccg gaggcacatt ggcagctcgg gctgcaatat
      601 cgtttcgggc aagggacgaa agtcgacacg gcacaggccg tcaatcattt gcgcgccgcc
      661 gcacaacaag gatacattcc tgcctacacc ccacttgccg agctcatcct acctacggct
      721 cctgatgaag ccgttcactg gtttcaacag gccgcacagg aaaatgaccc cgatgcccat
      781 gccgcacttg ccgacatcta cctgcaaggc aagcatctgg aaagaaacca caaacttgcc
      841 ctgcatcatg ccgaagcagc cgccgccgaa cgccatcccg aaggtttgcg gatactgggc
      901 gacatctgcc gctacggttt gggcatagcc cccgatacgg aaaaagcccg gcattattat
      961 cggcaggcag ccgaagccgg cagcctttcc gcctatcaga aactcatatc cgacagcgcg
     1021 ttaaaccatc ctgaccaata cggcggcatt aaagattccg ccatcaggcg gcaaagggca
     1081 gaacggcttt atcaaaaagc ccaagccctg cattacggat tacaatgcgc gcccgaatac
     1141 gcagccgcgc tcaaactcta cacagaagcc gcagaactcg gacacagcaa agcccaaacc
     1201 aatctgggca gcatgtatta cttcggacag ggtatgaccg ccgactacaa tgaagcacgc
     1261 aaatggtttg aaaaagccgc cgcgaaaaaa gacagtatgg cgttctacaa cctcgcctgc
     1321 atccattaca gcggacacgg cgtcgagccg gacaaagaaa aagcctgccg ctacctgcaa
     1381 gaagccataa acaacggata cgggcaaaaa agcgtcctgc aagaactgct gcaacaatgg
     1441 caaaatgccg tctgaacagc gttacaccta ccctgccgaa acgaaacagg tataatcgcc
     1501 cctttccctt cccgccgtcc gaacaggcat ttcacattca gacggcatcc tgattgcaca
     1561 agcgtacgaa agcattatga cagacaccgc cgagaaccaa acacaaaaca actggcaagc
     1621 cggacacccc cgcagcatcc gcagcttcgt cctccgccaa agccatatga ccgccgcgca
     1681 gcaacgcgcc atcgatacct tatgggacag cttcggcatc gactaccaag caacaccggc
     1741 cgatcttgat gcccgtttcg gaagcagccg acccaaaatc ctcgaaatag gcttcggtat
     1801 ggggacggca accgcagaaa tcgcccgccg cctgcccgaa accgacttcc tcgccatcga
     1861 cgtacacggt cccggcgtag gcaacctgct caaactcata gacgaaaacc atttagaaaa
     1921 catccgcgtg atgcggcacg atgccgtaga agttgtcgaa aatatgctgc aagacggctc
     1981 gctcgacggc atccacatat tcttccccga cccgtggcac aaaaaacgcc accacaaacg
     2041 ccgtctgata caagccccct tcatcgccaa actactgccc aaactcaaaa ccggcggcta
     2101 tatccacctg gcgacagact gggaagaata tgcacagcag atgcttgaag tcctcagtag
     2161 cttcgacagc ctgcaaaata cggcggcaga ctacgccccc accccggact accgccccga
     2221 aaccaaattc gaagcgcgcg gcaaacgcct cggacacggc gtttgggact tggtattcaa
     2281 acggatcgga taacaaacca ctgtttgaaa atgccgtctg aaacatgttt gcttacagac
     2341 ggcatttttt caagataaag cagcaagtga tgtttcgata taaagtttaa aacaatagtt
     2401 tgaacggcaa aacgcgtgtg taccgcacgc atccttatag gttttatgca catcggtttt
     2461 aaagtttgtg ccgcccgcag gtagtatgtg atagctacgc acgcggttgg tgtgatgtag
     2521 gctacggctt gctggttaca accgtaaaaa agtaagtgcc gccattgcgg taaaaacgaa
     2581 gggatttcat agtgttatgc tcgtaatgat tttgtagatt ggattctcga atccgacctt
     2641 ttgggcattg ctgcaatgga ttgcaacgac gggaatgttg aaggttttgt cggatacaag
     2701 tatccgacct acgcttgttg ctatatatct tttgatttaa tgactaaata tgacaaagtt
     2761 aaagtgcaga ttacagcaag catgatatgc ttctttaggc ttttatcatt ccatgatata
     2821 gatatttctt ccttttcatt ttctttataa aattttaaac ctatatcacc atttttccat
     2881 tcctggtggt ttactatgat tttattttta aaagaatctc ttaaactttc atgtaaagag
     2941 ttaaattttc ttgatttact tcccttagta catggtgagc aattgtattt ctaattttat
     3001 ttaatctctc ccctatatca tatacttcgc taaataagcc aagattacgc gcaattttta
     3061 gttttgtgcg aaatccaatt tgtgtatcat tgaaaaaatc ttctttatta cattttgcat
     3121 atatccatgc ctctaaaatt ctttcaaaaa ataaatgtgt tcgtaagatt gaacctattt
     3181 catcctgtgt ttcaatagct tctttcacga tatcaaaaat cttataatca tcaaaatttg
     3241 aatttttaat aaataattct aaattaaaat ctaattgaga cataataagt gcccatttca
     3301 aaaataaatc tatattctag ttaatataat agttattcta atatctaaat taaataataa
     3361 actactattt ttatatccac gacaaagtct aagtctcact ccgccccaaa caacaaattc
     3421 tctttaatat ccctaatcct atcccgcaac acagccgcct cttcaaactg caaatcccta
     3481 gccgcctgct gcatggcttt ttcgagtttg gcgatttctt taatcgcatc ttcttcgttg
     3541 tgaatctcgc ccactttaac cttgttttta cctttcagac ggcctttact gccgtcttct
     3601 tcgtggtaca cgccgtcgat gatgtctttg acctgttttt taatctgctg cggcacgatg
     3661 ccctgttctt cgttgaattt aatctgtttt tcacggcggc gttcggtttc gtcgatagcg
     3721 gctttcatgg agtcggtaat tttgtcggcg tacaggatgg cgacgccgtt cacgttgcgc
     3781 gcggcgcggc ctatggtttg aatcaggctg cggtgggagc gcaggaagcc ttctttatct
     3841 gcgtcgagga tggcgacgag agagacttcg gggatgtcga ggccttcgcg taagaggttg
     3901 atgccgacga gtacgtcaaa caggccgagc cgtaaatctc taatgatttc aacgcgctcg
     3961 acggtgtcga tgtcgctgtg caggtagcgc actttgatac cgagttcgct gtaatagtcg
     4021 gtgagttgct ccgccatgcg tttggtgagg gtagtaacga gtacgcgttc gcctttttca
     4081 atgcggtcgt tgatttcgct cattaagtcg tcgacttggg tggcaacggg gcggatgatg
     4141 atttggggat caaccagccc tgtggggcgg acgacttgtt cgaccacttg tccggcgtgt
     4201 tcttcttcgt atttggcggg ggtagcggaa acgaagatgg tttgcggcat gactttttca
     4261 aattcgtgga atttgagcgg gcggttgtcg cgggctgaag gcaggcggaa gccgtagtcg
     4321 acgaggtttt gcttgcgcga tgcgtcgcct ttgtacatgc cgccgatttg ggttacggta
     4381 acgtggcttt cgtcgatgaa catgatggcg ttgtcgggca ggtagtccat cagcgtaggc
     4441 ggcggttcgc cttctttttt gccggaaaag tggcgggagt agttttcgat tcctttgcag
     4501 aagcccattt cgtagagcat ttcgaggtcg aaacgggtgc gctgttcgat gcgttgttgt
     4561 tcgacggggc gttgttcgcg ggcgaaaaat tcgatgcgtt cgcgtaattc ttctttgatg
     4621 gactcgcagg cgcgcaagac ggtgtcgcgc ggggtaacgt agtggctgga cgggaagacg
     4681 gtgtagcggc cgacgcgctg gataaggctg cctgaaagcg ggtcgaacat atcgaggcgg
     4741 tcgatttcgt catcaaacag gctgatgcgt aaggcgtttt cggagctttc ggcggggtac
     4801 acgtcaatca cgtcgccgcg cacgcggaag ctgccgcgtt tgaagtccaa atcgccgcgt
     4861 tcgtattgca tggaaacgag cgtggcgatg atgtcgcgct gctcgatggt atcgccttct
     4921 ttgacggaca acaccatttg ttgatactcg gtcgggtcgc cgataccgta aatggcggac
     4981 acggtggcga cgataatcac gtcgttgcgc gtcattaggt ttttggtggc ggaaaggcgc
     5041 atctgctcga tgtgttcgtt gatcgcgctg tctttttcga tgaacaaatc gcggctgggc
     5101 acataggctt cgggctggta atagtcgtag taggagacga aatattccac tgcgttttcg
     5161 gggaaaaatt cgcgcatttc ggcgtaaagc tgggcggcaa gggttttgtt gtgcgccatg
     5221 atgatggcgg ggcggccgct ttgggcgatg acgttcgcca tggtgtaggt tttgcccgaa
     5281 ccggttacgc cgagcagggt ttgataggca aggccgtctg aaagcccttc gagcaggcct
     5341 gcaatggcgg tgggctggtc gcctgcgggc gggaagggtt ggtggagttt gaagggggaa
     5401 tttgggtatt ggataacttc cataatcttg cctgtgatgc gtttgcggac aaagcgtgca
     5461 gtagggatgg gtcggaaacg tctttcagac ggcataaggc ggtgaaatcc tgaatgtatg
     5521 ccgtctgaaa cccaatcgct accca
//
LOCUS       DAXHNE010000130         5498 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.130, whole genome shotgun sequence.
ACCESSION   DAXHNE010000130 DAXHNE010000000
VERSION     DAXHNE010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.130"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            109..804
                     /gene="pgl"
                     /locus_tag="ACFM4Z_001687"
     CDS             109..804
                     /gene="pgl"
                     /locus_tag="ACFM4Z_001687"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFC6021690.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            785..1771
                     /locus_tag="ACFM4Z_001688"
     CDS             785..1771
                     /locus_tag="ACFM4Z_001688"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFC6021691.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGTSPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            1821..2669
                     /gene="hexR"
                     /locus_tag="ACFM4Z_001689"
     CDS             1821..2669
                     /gene="hexR"
                     /locus_tag="ACFM4Z_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFC6021692.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            2790..4436
                     /gene="pgi"
                     /locus_tag="ACFM4Z_001690"
     CDS             2790..4436
                     /gene="pgi"
                     /locus_tag="ACFM4Z_001690"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC6021693.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEV
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGPQKHDSSTERLINLYLQTNRK"
     gene            4579..5001
                     /locus_tag="ACFM4Z_001691"
     CDS             4579..5001
                     /locus_tag="ACFM4Z_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC6021694.1"
                     /translation="MALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEAARVSEWEE
                     RYKLSRSEFEQFWKGLPQTVQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKVSEL
                     ACKTVETEARLEELYNRKKALIDEMVREADKKELPKRL"
     gene            5046..5186
                     /locus_tag="ACFM4Z_001692"
     CDS             5046..5186
                     /locus_tag="ACFM4Z_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021695.1"
                     /translation="MPEYRNWQNGLNAKCADGDEYGVAQSDCRTREINAKTKEIQGYL
                     ID"
     gene            complement(5367..5498)
                     /locus_tag="ACFM4Z_001693"
                     /pseudo
     CDS             complement(5367..5498)
                     /locus_tag="ACFM4Z_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153708080.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         1386 a         1522 c         1444 g         1146 t
ORIGIN      
        1 cagtcccgat atttggtatc aaaagcataa acaactgttc gaccggcata ttgctcaacc
       61 cgtttccata acggaatatg ccgtctgaaa taaaaaaagg acacaaatat gtttgtttgg
      121 cacgaatacg aaaatgcggc agaagcggcg cagtctttgg ctgacgcagt ggcggatgct
      181 ttgcagggcg cactggacga gaagggcggt gcggtgttgg cagtttccgg cggacgttcg
      241 ccgattgcat ttttcaacgc cctgtcgcaa aaagatttgg attggaaaaa cgtcggcatc
      301 accttggcag atgaacgcat cgtgccgacc gtccacgccg acagcaatac cggtttggtg
      361 cgcgaatacc tgttgaagaa caaagcggaa gcggcaatgt ggattcctat ggtggaagac
      421 ggaaaaactg aaaccgaatt acatcccgat gctgttgtcg attatgcact gaaacattac
      481 aaacagcccg atgttttggt tttgggtatg ggaaacgacg ggcatacggc ttcgattttc
      541 ccgaaagctc cacagtttca gacggcaatc gacggttcgg caggtgtcgc gctggtgcat
      601 accacgcccg ttaccgcgcc gcacgagcgc gtcagtatga ccttggatgc gattgcccat
      661 acggggcatg tgtttttggc gatacggggc gaagagaaaa aagccgtgtt cgaccaagcc
      721 gcacaaggcg aaaaccgcga atatccgatc aacctcgttt tgaaccatca aggagtgaac
      781 tgccatgtct tctacgccga ataaacaagc cggatatccc cgactggtcg ccgatatcgg
      841 cgggacgaat gcacgctttg cgctggaaac cgcgccgcgc gtcattgaaa aagccgccgt
      901 gcttccgtgt aaagactacg atacggttac cgatgcggtg cgtgcctatc tgaatcaaag
      961 cggtgcaaca gccgtacggc acgcggcatt tgccatcgcc aacccgattt tgggcgactg
     1021 ggtgcagatg accaaccacc attgggcgtt ttccatcgaa accacccgtc agactttggg
     1081 gctggacacc ctcatccttt tgaacgactt taccgcgcag gcattggcgg taacgcagac
     1141 ttcaagcaaa gacctgatgc aggtaggcgg gcaaaagcct gtcgaatttg cccccaaagc
     1201 cgttatcggc cccggtaccg gcctgggcgt gagcggattg gtgcacagcc acgcaggctg
     1261 ggtggctttg gcgggcgagg gcgggcatac cagtttcccg ccgtttgacg atatggaagt
     1321 gctgatttgg cagtacgcca aaaacaaata cggccatgtt tccgccgaac gctttttgag
     1381 cggcgcgggc ttgagcttgg tttacgaggc tttggctgca aaacagaaag ccaaacccgc
     1441 caaactgatg ccgtctgaaa tcacggaaaa ggctttgagc ggcacgtcgc ctttgtgccg
     1501 tcagactttg gacatcttct gcgccatgct cggcacggtt gcttccaacc tcgccctgac
     1561 gctgggcgcg cgcggcggcg tgtacctgtg cggcggcatt attccccgcg tgttggaata
     1621 cttcaaaact tccccgttcc gcagccgttt cgagaacaag ggcaggtttg aagcatatct
     1681 tgccgcgatt cccgtgtatg tcgtcttgag cgagtttccc ggaatttccg gtgcggctgc
     1741 ggctcttgac aaccatttga gaaacgttta accacagcgg ctccttgcag cggggctgca
     1801 ttatcgaagg gcatatcatt atgttaagca aaatcagcga atcactggca aacctttccg
     1861 gtgcggaacg caaagtcgcc gaatgtgcat tggcggaacc caaatggttc gtccatgccg
     1921 ccgttgccga aattgccgaa cgtgcgtccg tcagccagcc gaccgtcatc cgattctgcc
     1981 gcagcttggg ttataaaggg ctgcccgagt tcaagctcgc cttgtccgcc agcatcggtc
     2041 atgagggtat gccctatgtc cacgaagaac tcaacgccga cgacgatatg gcaagcgtgg
     2101 tcgagaaagt gttgggcaat gccgccgcct cgctgttggg cgaacgccgc ttcctgaaag
     2161 agtcggagct ggaaaacgcc attgccacgc tgatgcacgc ccgtcgcgtc gagttttacg
     2221 gtgtcggcaa ttccggcatt gtggcacagg acgcgcagca taaatttttc cgtttcggca
     2281 tgtccaccgt cgcctatgtc gatacgcaca cgcagctgat ggcggcatct gttttgagcg
     2341 atcaggatgt tttggttgcc atttccaaca cgggttcgtc tatcgaactt ttggatgcgg
     2401 tcagcatcgc caaagaaaac ggcgcgtctg tcatcgcact gacccgcaac gattcgcctc
     2461 tggcgcaact tgccgactgc gtgttgagcg ttgccacaca ggaaaatgcc gaactctaca
     2521 cgcccatggt ttcccgcctc ttgcagcttg ccgtcatcga cattctcgcc atcggacttg
     2581 ccctgcgctt gggcgatgct gccagcctgc aattgcagaa aagcaaaaaa agcatacaca
     2641 acaagcacat cgattacgac aaagattgac cttcagacgg catcccacaa atgccgtctg
     2701 aaatgccgaa caacggtcgt cggcggcttg cggcagtttc cggcagcctt ttcatcccac
     2761 aacaaaaaac ctcattcagg agcatataga tgaaacacct tcacgactta cccgcatggt
     2821 cgaaattgtg gaatcacttt gacgacagca aaacattgca tatgcgcgaa atgttcgagc
     2881 aagacccgca gcgtgcggaa cgctactggc tgcaagtcgg cggactgacg ctggactact
     2941 ccaaaaaccg catcaacgac gaaaccatgt cgcttttgtt cgagcttgcc cgagaagcag
     3001 gcgtgccgga gcggatgcgg cagatgttcc acggcgaaaa aatcaatacc accgaaaacc
     3061 gcgccgtcct gcatgtcgcc cttcgcaacc gcaccaattc gccgattgtg gttgacggtg
     3121 aagatgtgat gcccaaagtc aaccgcgttt tgcaacgtat gggcgaattt gcacacgaag
     3181 tccgcagcgg aagctggctg ggctatacca accaagtcat taccgacgtt gtcaacatcg
     3241 gcatcggcgg atcggatttg ggtccgctga tgatgtgtac cgcgctcaaa cctttcggtc
     3301 atccgcgcct caatatgcac ttcgtctcca acgtggacgg ctcgcaactg cgcgacgtat
     3361 tgtccaaagt ccaccccgaa accacgttgt tcatcatcgc ctccaaaaca tttaccacgc
     3421 aggaaacgct gaccaacgcg ctgaccgcgc gcgaatggtt tttgaatcat gcgggcgacg
     3481 aagaagccgt tgccaaacac ttcgccgccg tttccaccaa tcaaaaagcc gtcgccgaat
     3541 tcggcatcga caccgccaat atgtttgaat tttgggattg ggtcggcggt cggtacagcc
     3601 tgtggtccgc catcggattg ccgattatgc tgtatctcgg cgaagaaaac ttcattgaaa
     3661 tgctcaacgg cgcgcacctg atggaccaac acttcatcaa cacaccgctc gagcgcaacc
     3721 tgcccgtcat tctcgccctc atcggcatct ggtatatcaa ctactacggc ggcggcagcc
     3781 acgtcatcgc gccttacgac caacatttgc accgcctgcc caaattcatc cagcagctcg
     3841 atatggaaag taacggcaaa caggttacgt tggacggcaa agcagtcgga cacgaaacct
     3901 cgccgattat ctggggcgaa acgggcatta acggccagca cgcctttttc caactgctgc
     3961 accaaggcac gcacattacc cccatcgacc tgattgcctc gcttgaaaaa cgcagcaacc
     4021 tgcccggaca ccacgaaatc ctgcttgcca acgtcttcgc ccaagcagaa gcctttatgc
     4081 gcggcaaaac ccccgacgaa gtccgcgccg aactcaaagc gcagggtatg gatgaggtgc
     4141 gcatcgaaga gctggtcccg cacaaaacct tctccggcaa ccgcccgacc aacctcattc
     4201 tcatggacaa ggtcaacccg cgcaatatgg gcagcctgat tgccatgtac gaacacaaaa
     4261 ccttcgtaca aggcatcatt tggggcatca acagcttcga ccagtggggc gtggaactcg
     4321 gcaaacaact ggctaaaacc attttgggcg aactgaccgg cgaaaccggg ccgcaaaagc
     4381 acgacagttc gaccgaacgc ctgatcaacc tctacctgca gaccaaccgc aaataaaacc
     4441 tgcggaaaaa tgccgtctga acgccgaccg tttcaaacgg catttttatc gaacaggaaa
     4501 accgtcggta aacttgcaga gcgtgtgcaa tcccgatatg atggtttgca taaatttaaa
     4561 catatatgtt ccgcagctat ggcactgatt aaagagccgt tggacaaagc gaaacaaagg
     4621 aacgaagaac ttgaagcggc agaagaagcg gcggcgcagg aggcattggg tcgggagcag
     4681 gaagccgccc gcgtatccga atgggaagaa cgctacaagt tgtcgcgcag cgagttcgag
     4741 cagttctgga aaggattgcc gcaaactgta cagaataaac tgcaagcttc gcagaaaaca
     4801 tggaaaagcg gtatggacaa aatctgtgcc aacaatgcga aagccgaagg tgaaacgcca
     4861 aacggcataa aagtcagcga actggcatgc aaaacggtgg aaaccgaagc acgcttggaa
     4921 gagctgtaca accgtaaaaa agcccttatc gacgaaatgg tcagggaagc ggacaagaaa
     4981 gaactgccaa agcggctctg aacagcgcgg ttcaggcact gcccgccgat attgccgaaa
     5041 ccgtcatgcc cgagtaccgc aactggcaaa acggtctgaa tgccaaatgc gctgacggcg
     5101 acgaatacgg cgtggcacaa tccgactgcc gtaccagaga aatcaatgcg aaaaccaaag
     5161 aaatccaagg ttatctgatt gactgaaact tggatgcggg aatgtcggcg gcttttgcgt
     5221 ttttgtcgtt tttatagtga attaaattta aaccgataca tcgttggctc gccttagctc
     5281 aaagagaacg attctctaag gtgctgaagc accaagtgaa tcggtccgta ctatttgtac
     5341 tgtctgcggc ttcgccgcct tgtcctgatt tttgttaatc cactatatct ttttccactt
     5401 atctgcgctt tcactatttg ccctttcagg ctgcgggcat agggacggaa caggtagcgg
     5461 tcaaatcctg tttcatccaa ataaacacgt tggtagtc
//
LOCUS       DAXHNE010000131         5481 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.131, whole genome shotgun sequence.
ACCESSION   DAXHNE010000131 DAXHNE010000000
VERSION     DAXHNE010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.131"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            116..637
                     /locus_tag="ACFM4Z_001694"
     CDS             116..637
                     /locus_tag="ACFM4Z_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
                     /protein_id="HFC6021696.1"
                     /translation="MSCFSIKRMSAFRARITAFFAAFVFLTAALPAYAERLPDFLAKI
                     QPSEIFPGADRYSKPEGKPMVARVYKGDEQLGLVYITTDAVNTRGYSSKPIDTLMALA
                     NDGTIAGAKLVDHHEPIMLIGIPHLPAPGRAIRLNWLPAYIKPNFISTNRLRLKGLPT
                     VPQPSKETGAGVP"
     gene            526..1725
                     /locus_tag="ACFM4Z_001695"
     CDS             526..1725
                     /locus_tag="ACFM4Z_001695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HFC6021697.1"
                     /translation="MASGVYQTKLYIDKPITIEGPADRSATIEGDRSGRTIAVHAPDV
                     TLRNLTVTRSGMSLPAMDAGIYLEETAPRALIEHNNILDNSVGVYLHGSADAMVRENK
                     IVGDATLRVNERGNGVTVWNAPGAQVVGNDISKGRDGIFSNTSTHNTYKNNRFSDLRF
                     AVHYMYTNDSEISGNISVGNNMGYVLMFSERLKVFDNIAVGSRDQGIMLNYVNYSDIH
                     DNIINKAGKCVFAYNANYDKLSANHFENCQIGIHFTAAIEGTSLHDNSFINNESQVKY
                     VSTRFLDWSEGGHGNYWSDNSAFDLNGDGFGDSAYRPNGIIDQIIWRAPVSRLLMNSP
                     AISIVKWAQAQFPAVLPGGVVDSKPLMKPYAPKIQTRYQAMKDELLKEVETRQSEWGR
                     AENGSLN"
     gene            1783..2397
                     /locus_tag="ACFM4Z_001696"
                     /pseudo
     CDS             1783..2397
                     /locus_tag="ACFM4Z_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709597.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2414..2545
                     /locus_tag="ACFM4Z_001697"
                     /pseudo
     CDS             2414..2545
                     /locus_tag="ACFM4Z_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2542..3036
                     /locus_tag="ACFM4Z_001698"
     CDS             2542..3036
                     /locus_tag="ACFM4Z_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HFC6021698.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDT
                     YIFK"
     gene            complement(3124..4785)
                     /locus_tag="ACFM4Z_001699"
     CDS             complement(3124..4785)
                     /locus_tag="ACFM4Z_001699"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HFC6021699.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEVGAHSLAGAVIDPIALNELISDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GIGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQIIERFQLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGILVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVEGFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(4915..>5481)
                     /locus_tag="ACFM4Z_001700"
     CDS             complement(4915..>5481)
                     /locus_tag="ACFM4Z_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC6021700.1"
                     /translation="PVAYGKIKVQSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQ
                     KLTEEDVLKKLDKEQMPASFEDMRRIMPDLGFEAKGYALSFEQLAQLQIPVIVYLKYR
                     KDDHFSVLRGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKGRDA
                     GGDKAFFTRSPKRQTEFAVGQVKWWRAY"
BASE COUNT         1306 a         1489 c         1400 g         1286 t
ORIGIN      
        1 tatcccgtat gcggattggt tttaagattt gtaaatttga tttgcatcaa aaaatcgccg
       61 atagatgatt catataatat caatattaaa gagtatcggt atatcgggga tagtcatgtc
      121 ctgtttttca atcaaacgta tgtccgcgtt tcgggcgcgg ataacggcgt tttttgccgc
      181 ctttgtcttt ttgacggcgg cactgcccgc ttatgcggag cgtctgcctg attttctggc
      241 gaaaatacag ccttcggaaa tttttccggg tgcggaccgt tacagcaagc cggaaggtaa
      301 gcctatggtt gcccgcgttt acaaaggcga tgagcagttg ggcttggtct atatcacgac
      361 cgatgcggtc aatacgcgcg gttattcgag caaaccgatc gatacgctga tggctttggc
      421 aaatgacggc acgatagccg gagcgaaact ggtcgatcat cacgaaccga ttatgctgat
      481 cggtatcccg catttgcccg cgcccgggcg ggcgatacgc ttaaactggc ttccggcgta
      541 tatcaaacca aactttatat cgacaaaccg attacgattg aagggcctgc cgaccgttcc
      601 gcaaccatcg aaggagacag gagcgggcgt accatagccg tacacgcgcc ggacgtaacg
      661 ctccgcaacc tgaccgttac ccgttccggt atgagcctgc ctgcaatgga tgccggtatt
      721 tatctcgaag aaaccgcccc gcgcgccctg atcgaacaca acaatatttt ggataattcg
      781 gtcggcgtat atctgcatgg ttctgccgat gcgatggtgc gggagaataa aatcgtcggc
      841 gacgcgactt tgcgcgtgaa cgagcgcggc aatggcgtta ccgtttggaa cgcgcccggc
      901 gcgcaggtcg tcggcaacga tatttccaaa gggcgggacg gcattttttc caataccagc
      961 acgcacaaca cctataaaaa caaccgcttc agcgatttgc gtttcgccgt ccactatatg
     1021 tacaccaacg acagcgaaat cagcggcaat atttccgtgg gcaacaatat gggctatgtg
     1081 ctgatgtttt ccgagcggct caaagtgttt gacaatatcg ccgtcggcag ccgcgaccaa
     1141 ggcatcatgc tcaactatgt caactattcc gatattcacg acaacattat caacaaagcg
     1201 ggcaagtgcg tttttgccta caatgccaac tacgataaac tgtccgccaa tcattttgaa
     1261 aactgccaaa tcggcataca ctttaccgcc gccatcgaag gcacgtcact gcacgacaat
     1321 tcctttatca acaacgaaag ccaggtcaaa tacgtcagca cgcgctttct cgactggagc
     1381 gagggcggac acggcaacta ttggagcgac aacagcgcgt tcgatttgaa cggcgacggc
     1441 ttcggcgaca gcgcgtaccg gcccaacggc atcatcgacc aaatcatctg gcgcgcgccc
     1501 gtatcgcgtc tcttgatgaa cagtcccgca atcagcatcg tcaaatgggc gcaggcgcag
     1561 tttcccgccg ttctgcctgg cggcgtggtg gacagcaaac cgctgatgaa gccttatgcc
     1621 cccaaaattc aaacccgtta tcaggcgatg aaggacgagc tactcaaaga agtcgaaacg
     1681 cggcagtcgg aatggggcag ggcggaaaac ggttctttga actagtctgc ttcagacggc
     1741 atccggattc aaatgccgtc tgaaaacaca aaaggaacaa ccatgaccac acatcatgtc
     1801 gaattgagga aggtaaccaa acggttcggg gcgcaaaaag ccgtcaacca agtcgatttg
     1861 gttttgaagg caggagaaag cgtcgggctt gccggacaca acggcgcggg caagtccacc
     1921 attatgaagc tgatactcgg gctgattacc ccgaccgaag gcgaagtgat gcttttgggc
     1981 gaacgtaccg gtagcaaagc gggggcgcgg cttcgcagcc aaatcggcta cctgcccgaa
     2041 accgttgcgc tgcacccttc gctgatcggc atcgaaacgc tggattttta tgccaaactt
     2101 aaaaaacagc cgctcacgca gaaccggggg ctgcttgagc gcgtcggcat ttcacaggcg
     2161 gcacaccgcc gcgtcggcac ttattctaaa gggatgcgcc aacgccttgc cttggcacaa
     2221 gccctgctgg gcgagcccaa agtcctgctg tttgatgaac cgacaaccgg tcttgaccct
     2281 gcatcacgac aaatgtttta cgaagtcgtg cgcgaactca acgggcgcgg cgcgaccgta
     2341 ttgctcagca cccacgccct tgccgagttg gacgggcacg ccgaccgcat tatcgtggat
     2401 taaatttaat ccactatatg cgggtatggc gggtttgagc ggacaaatca gcctgaccgt
     2461 ccccgttttg ctgaccgctc aggttttatg ggttatcatt ccgcttgttt tggcagccgg
     2521 aatttttaga aagcgacaaa tatgaaaaaa accctgttgg caattgttgc cgtttccgcc
     2581 ttaagtgcct gccggcaggc ggaagaggga ccgccgcctt taccccggca gattagcgac
     2641 cgttcggtcg gacactattg cagtatgaac ctgaccgaac acaacggccc caaagcccag
     2701 attttcttga acggcaaacc cgatcagccc gtttggttct ccaccatcaa gcagatgttc
     2761 ggctatacca agctgcccga agagcctaaa ggcatccgcg tgatttacgt taccgatatg
     2821 ggcaatgtta ccgattggac gaatcccaat gccgacacgg agtggatgga tgcgaaaaaa
     2881 gccttttacg tcatcgacag cggctttatc ggcggtatgg gtgcggaaga cgcgctgccg
     2941 ttcggcaaca aagagcaggc tgagaaattt gcaaaggata aaggcggtaa ggttgtcggt
     3001 ttcgacgata tgcctgatac ctatattttc aaataatatt gaaaaaaccg gccgaaactt
     3061 taaaaacgag tttcggtcgg tttttctttt cttgttcggt atagtgtcgg caggaaagaa
     3121 ccttcacatc ccgccgtaat tcggcccgct cgcgccttcg gggcaaatcc aagtgatgtt
     3181 ttgcgtcggg tctttgatgt cgcaggtttt gcagtgcacg cagtttgccg cgttgatttg
     3241 caggcgcgga ttgccgtttt cttcaacaat ttcgtacacg ccggccggac aatagcgcgt
     3301 ttcgggcgag gcgtattctt tgtagttcac gtctatcatc gtttgcggat tgttcagcac
     3361 caaatggtcg ggctggtttt cttcgtgcgc gagattggca aggaagacgc tgctcaagcg
     3421 gtcgaaggtc aacacgccgt cgggtttcgg ataatcaatc ggcttacacg cggcggcttt
     3481 tttaagctgc tcgttgtctt tgccgtgatg tttcaaggtc cacggggctt tgcctctgaa
     3541 aatcatctga tcgatgccgg tgtagattga gccgaggtaa acgccccatt tgaatgacgg
     3601 acggacattg cgcgcggcgt aaagctcttg atacagccag ctttgttcaa aacgttgctg
     3661 ataatccgcc gcctctttgc cgctgtcgaa accctccact tcttcaaggt tttccaacaa
     3721 ggggaacacg gcttcggcgg cgagcatggc ggatttcatc gcggtatgaa tgcctttgat
     3781 gcgcggcata ttgaggaaac ccgccgcatc gccgaccaaa atgccgcctt tgaacgagag
     3841 cttcggcaaa ctttgcaaac cgccttcaat cagcgaacgc gcgccgtaag caatgcggcg
     3901 gccgccttca aaggttttgc ggatttcggg atgggttttg aaacgttgga actcttcaaa
     3961 cggcgacaga taaggatttt gatagtccaa accgaccacg aagccgacgg cgactttgtt
     4021 gtcgtcgaaa tggtaaacaa acgcgccgcc gtaggttttg ctgtccagcg gccagcctgc
     4081 gctgtgcacc accaaaccgg gctgatgctg ttcggacggc acttcccaaa cttctttaat
     4141 gcccaagccg taagtttgcg gctggctgtt ttggtcgagt tggaaacgtt cgatgatttg
     4201 tttggaaagc gaaccgcgac aaccttcggc aaacagggtt tgctgcgccc aaagctccat
     4261 gccgggttgg aatgaatcgg tcggctcgcc gtctttgcca atgcccatat tgccggttgc
     4321 aatgcctttg accgaaccgt cttcgtgata cagcacttcg gcggcggcaa agcccggata
     4381 gatttccacg cccatatttt ccgcctgctc cgccaaccag cgcacgactt cgcccaagct
     4441 gacgatgtag ttgccgtgat tgtcgaaatt cggggtaatc ggcaggttga acgctttttt
     4501 ctcggtcagg aacaacactt tgtcctgcgt tactgtgcgt gtcagcggtg cgcctttttc
     4561 tttccagtcg gaaatcaact cattcagcgc aatcggatcg ataactgcgc cagccagcga
     4621 atgcgccccc acctccgaac ctttctccac cacgcaaacg ctgatttcgc gcccgttttg
     4681 ttcggcaagc tgcttgagtt tgatggcggc agacaaaccc gacgggcctg cgccgacaat
     4741 cacgacatcg tattgcatac tgtcgcgggt gatggattct gtcatggcgg ttcctgtgta
     4801 tttattattg aattgcaaat ccgtaattat acaacgggaa catatagtta ccaaatacaa
     4861 caaaggtcgt ctgaaaacca tattttcggt tttcagacga cctttgtcga aatttcaata
     4921 agcacgccac cattttacct gtccgaccgc aaactccgtc tgacgtttcg gactgcgtgt
     4981 gaaaaacgcc ttatccccgc cggcatccct ccctttcggc acaaccgcca aaatcttacc
     5041 tgccaaattt ccctcacggg tttgccaagc atccaaaaac tgcgccctgc tcatcgaaac
     5101 atgacccagc gacgggtcgg caagcaaaac cgtattgccg tctataccgc gcaataccga
     5161 gaaatgatca tccttgcggt atttcagata cacgatgacg gggatttgca actgtgcaag
     5221 ctgctcgaaa gacagggcat agcctttcgc ttcaaaaccc aaatcaggca taatgcgccg
     5281 catatcctca aacgacgcgg gcatctgctc cttatccagt ttttttaaca cgtcctcttc
     5341 cgtcagcttt tgcccgtaaa aattgttcaa aagcgtcgcc accgaagccg ccccgcagga
     5401 aaaatccaaa tcctgcttta caatattgaa atcgcgcctc tctttccaac tctgcacttt
     5461 gatttttcca taagcaacag g
//
LOCUS       DAXHNE010000132         5378 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.132, whole genome shotgun sequence.
ACCESSION   DAXHNE010000132 DAXHNE010000000
VERSION     DAXHNE010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.132"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            211..521
                     /locus_tag="ACFM4Z_001701"
                     /pseudo
     CDS             211..521
                     /locus_tag="ACFM4Z_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(578..1636)
                     /gene="trpD"
                     /locus_tag="ACFM4Z_001702"
     CDS             complement(578..1636)
                     /gene="trpD"
                     /locus_tag="ACFM4Z_001702"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HFC6021701.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQIMRGQVLPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVVEQMGANLNLTPEQVAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLEGREGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(1700..2290)
                     /locus_tag="ACFM4Z_001703"
     CDS             complement(1700..2290)
                     /locus_tag="ACFM4Z_001703"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFC6021702.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFRGLPNPVTCTRYHSLVIDRNTMPECLEVTAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLVEFQNFKPQKI"
     gene            2712..4988
                     /gene="znuD"
                     /locus_tag="ACFM4Z_001704"
                     /gene_synonym="tdfJ"
     CDS             2712..4988
                     /gene="znuD"
                     /locus_tag="ACFM4Z_001704"
                     /gene_synonym="tdfJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118820519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc receptor ZnuD"
                     /protein_id="HFC6021703.1"
                     /translation="MEQTTLKPIVLSILLTNTPLLAQAHETEQSVDLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDVDYDNPGLSCGFHDDDDAHAHAHNGKPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYHHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSATSEAVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGVHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
BASE COUNT         1383 a         1517 c         1236 g         1242 t
ORIGIN      
        1 gttagataag gggggaagat ttgaagcggt tgtcggcttc ctgccgtccg ctagcgcgtc
       61 cgtcatcacg ccggcaaccg cctttgtcat cccttgctta tcttccatgg tgcgaatcct
      121 caaaaacggg caaaaaaaaa gccctgttac ttgtagaaag taaaggacgt taatttttgt
      181 taatcgtccc ttcttaggga cgcaatatat aaggtttata ccgtcgttgt tcctaatgcg
      241 caatcagcga cattgttgcc aattatccga aagaaagtta agcctgatgg cattgtatac
      301 acggatacct ttcgtagtta tgatgtgctt gatattagtg aatttagcca tttacgtaag
      361 tttaacggca ttcccaaaga gcatttgggg ctgcatttaa agaaatgcca atggcatttt
      421 aaatagaatc tcaaattcag attttaagac aattagttaa tgggaatttg gtctggttat
      481 ctagtacagc cccaagtttt tatataaaaa cagtttggta agttctcttc gtgaagtgct
      541 tatgttttgc cacaaaagtt ccataatcat ggcggcatca cccaagctcc atctttccaa
      601 gaaaatggca tctttgttgt ggttgaaaac cgacaaactc ctcttttttc gatttggccc
      661 tgcctgaatc gatggcttcc cgtgcggcag atatgccgtc tgaaagcgaa gcagcgacat
      721 ttccggcata cagggcggcg gcggtgttga gcaatacgat atcgcgcgca gccccttctc
      781 ttccttccag cacctcattc attttcaaca aagattcctg agtattggca actttgattt
      841 catccaaatt gcggcgggtt tcgataccga aatcttctgg gcggatgtcg tattcgctga
      901 tttttccgtc tttgagctcg gcaacgcgtg ttttgcccgt cagtgtaatt tcatccaaac
      961 cgccctcccc gcaaacaacc aaaacgtgtt ttgaaccaag ttgttgcaag acccgcgaca
     1021 aaatgccgca caaatcggtg tggaacacgc ccaaaagctg gttcggcgcg cccgcaggat
     1081 tcgttaacgg acccaatatg ttgaaaatac ttcggaaacc gagcgaacgg cgtacagggg
     1141 cgacatggcg catggcactg tggtgattgg gcgcgaacat aaacccgatg ccggtctgcc
     1201 tgatactttg ggcaacctgt tcgggagtca ggttgaggtt tgcgcccatc tgctccacca
     1261 cgtcagccgc accgctggag gaagagaccg accggcctcc gtgtttggca accttcgcgc
     1321 ctgccgctgc ggcaacaaac atcgaagtcg tcgaaatatt gaaggttttc gcgccatccc
     1381 cgcccgtacc gacgatatcg accagcccct ctgcattctc cagcggcact tttgtcgcaa
     1441 actcgcgcat gacggctgca gctgcggtaa tttcggaaac ggtttcaacc ttgatacgca
     1501 atcctgtcaa aatggccgct atctgctccg gcagaacctg tcctctcata atctgacgca
     1561 tcaagtcggt catttcatcg taaaacaact cgttattgct gattaatcgt tcgatggcct
     1621 gttgcggtgt aatcattttt tgtcctccgt tcaatattcg gacgaaaatg ccgtctgaag
     1681 ggcttcagac ggcatcacgt cagatttttt gcggtttgaa gttttgaaat tcaactaaaa
     1741 aattgtttaa catatcatgt ccgtgttcgg tcaagagggc ttcggggtgg aactgcacgc
     1801 cctcgacggc atattcctta tggcgcacac ccataatctc gccgtcctca gtccaagccg
     1861 ttacttccaa acattcgggc atcgtattcc gatcgataac gaggctgtga taacgcgtac
     1921 aggtaaccgg attgggcaac cccctaaaca tacccttgcc cgaatgggac acgggcgaca
     1981 ccttaccgtg catcagcgtt ttggcgcgga ctatcctgcc gccgaacgcc tcgcctatcg
     2041 tctgatgccc gaggcacacg cccataatcg gcagccggcc ggcgaaatgg cgcatcgccg
     2101 ccacggaaat ccccgcttct ttgggcgaac acggaccggg gccgataacg agatattgcg
     2161 gattcaatgc ctcgatttcc tccaacgtaa tatcatcgtt gcggcgcacg gcaacttcct
     2221 gccccaattc tgcgaaatac tggacgatgt tgtaagtaaa actgtcgtaa ttgtcgataa
     2281 acaaaagcat tttgtgatta acctattgat ttataatttg tttgttatta actgcatttc
     2341 cgataccccc atccataccc tcttatatct tagcgtgccc gatgcgccct cgtgaacctg
     2401 gcgcagagcc tcgcgttctg acaagtacgc cattttgcca ccttttcgac cgttcgggaa
     2461 cgtgccgttt ttatgcactc ataaattttg gttatcccgc agccgaacat aatgggcaaa
     2521 cacagcggaa tctgcgtcaa catcaaccgc gcccccgaag gacggcggat acaaatggac
     2581 accgcccgat gccgcaaccg tttcatttga aaaacttttg cctatgccct ctgagtgttc
     2641 tcaaaatctt atgatataaa aaatgtaatg ttatataata acaaactttt gaaatgcgac
     2701 gagaagccaa aatggaacaa accactctca agcccattgt tttatcaatt cttttaacca
     2761 acacacccct cctcgcccaa gcgcatgaaa ctgagcaatc ggtggatttg gaaacggtca
     2821 gcgtcgtcgg caaaagccgt ccgcgcgcca catccgggct gctgcacact tcgaccgcct
     2881 ccgacaaaat catcagcggc gacaccttgc gacaaaaagc cgtcaacttg ggcgatgctt
     2941 tggacggcgt gccgggcatt cacgcctcgc aatacggcgg cggcgcgtcc gctcccgtta
     3001 ttcgcggtca aacaggcagg cggattaaag tgttgaacca tcacggcgaa acaggcgata
     3061 tggcggattt ttcgcccgat cacgccatta tggtagatac cgccttgtcg caacaggtcg
     3121 aaatcctgcg cgggccggtt acgctcttgt acagctcggg caatgtggcg gggctggtgg
     3181 atgttgccga tggcaaaatc cccgaaaaaa tgcctgaaaa cggcgtatcg ggcgaactag
     3241 ggctgcgttt gagcagcggc aatctggaaa aactcacgtc cggcggcatc aatatcggct
     3301 tgggcaaaaa ctttgtattg cataccgaag gcttgtaccg caaatcgggc gattacgccg
     3361 taccgcgtta ccgcaatctg aaacgcctac ccgacagcca cgccgattcg caaacgggca
     3421 gcatcgggct gtcttgggtt ggcgaaaaag gttttatcgg cgtagcgtac agcgaccgtc
     3481 gcgaccaata tggtctgcct gcccacagcc acgaatacga tgattgccac gccgacatca
     3541 tctggcaaaa gagcttgatt aacaaacgct atttacagct ttatccgcac ctgttgaccg
     3601 aagaagacgt cgattacgac aatccgggct tgagctgcgg cttccacgac gacgatgatg
     3661 cacacgccca tgcccacaac ggcaaacctt ggatagacct gcgcaacaaa cgctacgaac
     3721 tccgcgccga atggaagcag ccattccccg gttttgaagc cctgcgcgta cacctgaacc
     3781 gcaacgacta ccaccacgac gaaaaagcag gcgatgcagt cgaaaacttt tttaacaacc
     3841 aaacgcaaaa cgcccgcatc gagttgcgcc accaacccat aggccgtctg aaaggcagct
     3901 ggggcgtgca atatttacaa caaaaatcca gtgctttatc tgccacatcc gaagcggtca
     3961 aacaaccgat gctgcttgac aataaagtgc aacattacag ctttttcggt gtagaacagg
     4021 caaactggga caacttcacg cttgaaggcg gcgtacgcgt ggaaaaacaa aaagcctcca
     4081 tccgctacga caaagcattg attgatcggg aaaactacta caaccatccc ctgcccgacc
     4141 tcggcgcgca ccgccaaacc gcccgctcat tcgcactttc gggcaactgg tatttcacgc
     4201 cacaacacaa actcagcctg accgcctccc atcaggaacg cctgccgtca acgcaagagc
     4261 tgtacgcaca cggcaaacac gtcgccacca acacctttga agtcggcaac aaacacctca
     4321 acaaagagcg ttccaacaat atcgaactcg cgctgggcta cgaaggcgac cgctggcaat
     4381 acaatctggc actctaccgc aaccgcttcg gcaactacat ttacgcccaa accttaaacg
     4441 acggacgcgg ccccaaatcc atcgaagacg acagcgaaat gaagctcgtg cgctacaacc
     4501 aatccggtgc ggacttctac ggcgcagaag gcgaaatcta cttcaaaccg acaccgcgct
     4561 accgcatcgg cgtttccggc gactatgtac gaggccgtct gaaaaacctg ccgtccctac
     4621 ccggcaggga agatgcctac ggcaaccgtc ctttcatcgc acaggacgac caaaatgccc
     4681 ctcgcgttcc ggctgcgcgc ctcggcgtcc acctgaaagc ctcgctgacc gaccgcatcg
     4741 atgccaattt agactactac cgcgtgttcg cccaaaacaa actcgcccgc tacgaaacac
     4801 gcacgcccgg acaccatatg ctcaacctcg gcgcaaacta ccgccgcaat acgcgctatg
     4861 gcgagtggaa ttggtacgtc aaagccgaca acctgctcaa ccaatccgtt tacgcccaca
     4921 gcagctttct ctctgatacg ccgcaaatgg gccgcagctt taccggcggc gtgaacgtga
     4981 agttttaaaa tcggacaggc aaacaaaaaa ggttttcaga cggcattctg aaaacctttt
     5041 ttctttttaa tcaatttgtt aggaaatatg gcaaagacaa agaacaaacc actatgccat
     5101 cattcccatt ttctttgttt catcattctt gctttcacgc cgtcattctc gcaaaagcag
     5161 aaaaatcaaa agcagaaacc caagatcccg tcattcccgc gcaggcggga atccaattcg
     5221 ttcggtttca gccgtttccg ataaatcact gtaacgttaa gttcccggat tcccactttc
     5281 gtgggaatga cggcgtaaag gtttctgttt tttaagtttt aggtaactaa taagtcgtca
     5341 ttcccgcgca ggcgggaatc caattcattc ggtttcag
//
LOCUS       DAXHNE010000133         5331 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.133, whole genome shotgun sequence.
ACCESSION   DAXHNE010000133 DAXHNE010000000
VERSION     DAXHNE010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.133"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            304..1737
                     /locus_tag="ACFM4Z_001705"
     CDS             304..1737
                     /locus_tag="ACFM4Z_001705"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFC6021704.1"
                     /translation="MNEYSQLIKHPDISLPPVSDGIKVDNPATGETLAFVRKTDSDKL
                     KNLIQKAAAAQKLWAAKTALERADVLWRWYFLIKENKEALARLMTMEQGKGLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALAVLAYEAGVPQDLLPVVSGSASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDAFCRKLSEKAAALKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALSKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEVEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            1847..2635
                     /locus_tag="ACFM4Z_001706"
     CDS             1847..2635
                     /locus_tag="ACFM4Z_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HFC6021705.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVVEAFEKDRINVVTAAEVAVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            3003..4559
                     /locus_tag="ACFM4Z_001707"
     CDS             3003..4559
                     /locus_tag="ACFM4Z_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HFC6021706.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELAPQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            4576..5322
                     /gene="surE"
                     /locus_tag="ACFM4Z_001708"
     CDS             4576..5322
                     /gene="surE"
                     /locus_tag="ACFM4Z_001708"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFC6021707.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEKALWTLLAHFFKNPPQSPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHAD"
BASE COUNT         1244 a         1358 c         1524 g         1205 t
ORIGIN      
        1 gttcggattt ttgccatact ctaccacacg ttgcaactgc aatctttgct ccttatatat
       61 tgcaccccat caaagggctg cattactttt ctttctaaag attactatcg tataaaaaat
      121 atttttctta aacgacaggg attgcgcccg cgccatattc agacaccgcc cggatgttgc
      181 gctgcccgat cggatgcttc agacggcatc ggaagggttt gcagttttgg aatatgaaga
      241 tgataatgtc cgcgagattg acggcatttg aaagcggtta ttccgcatac cggaggatac
      301 gaaatgaacg aatattccca attaatcaag catcccgata tttcccttcc cccggtttca
      361 gacggcatca aagtcgataa tccggcaacg ggcgagactt tggcgtttgt ccgcaagacg
      421 gattcggaca agctgaaaaa cctgattcaa aaggcggcgg cagcgcaaaa attatgggcg
      481 gcaaaaaccg cgttggaacg cgccgatgtg ttgtggcgtt ggtatttttt gataaaggaa
      541 aacaaagaag cactggcgcg tctcatgacg atggagcagg gcaaaggcct gaccgaggcg
      601 cgcggcgaaa tcgattatgc ggcttcgttt gtgcgctggt ttgccgaaga ggcgcggcgg
      661 attgacggcg atgtgctgac gagtgtgaaa gcgtcgcaaa aactggtcgt gttgaaacag
      721 cctgtcggtg ttaccgccgc gattacgccg tggaactttc cgtccgcgat gattgcgcgc
      781 aaggctgcgc cagctttggc agtgggttgc gcgatgatag tcaaacccgc gtcgctcacg
      841 cctttgagcg cgtatgcgtt ggccgtgttg gcttacgaag cgggcgtgcc gcaggatttg
      901 ctgcctgttg tcagcggcag tgcttcggaa atcggccgtg aatttgccac aaatccgatt
      961 atccgcaaaa tcagtttcac cggctctacc gaagtcggcg cgaaaatttt tgccgacagc
     1021 gcggcagaca ttaaaaaact cagcttggaa ctgggcggca acgcgccgtt tatcgtgttt
     1081 gacgatgccg atttggacaa agccgtcgaa ggtgcgctcg ccagcaagtt ccgcaacagc
     1141 ggtcagacct gcgtctgcac caaccgcgtt tacgcccaat cgggcattta cgacgcattt
     1201 tgccgcaaat tgagtgaaaa agcagccgcg ctcaaattgg gcaacggctt ggaggatggc
     1261 gtcaaccaag ggccgctgat tgaggaaaaa gcggtagaaa aagtcgagca gcacatcgcc
     1321 gacgcgctct caaaaggcgc ggtctgtctg accggcggca aacgcagcgc attgggcgga
     1381 acgttttttg agccgaccgt cttaagcggc gtaacggcgc aaatggcggt ggctcgcgaa
     1441 gaaaccttcg ggcctttgtg tccggtattc cgttttgaaa ccgaagtcga ggtcatcgag
     1501 gctgcgaaca atacggaata cggtttggcg gcctaccttt tcaccgccga caccgcccgc
     1561 caatggcgcg tcggcgaagc cttggaatac ggtatggtcg gcatcaatac gggcttaatc
     1621 agcaatgaag cggcaccgtt cggcggcgtg aaacgcagcg gtttgggacg tgaaggcagc
     1681 aaatacggtg cggacgaata tctggaattg aaatatctgt gtatggatgt cgggtgacgg
     1741 atgccgtctg aacggcaggg tttcagacgg tgccgcattt taagcagtct ctatctgttg
     1801 tacaatgcgc cctgttttta cggttatttt tgatgtgaat taagatatga tggaaaacgg
     1861 aaaaacagtc ccgagatggc gttttgcctt gaaaagtgcg ggctggcacc tcttaatcag
     1921 cctgtcggtt gcagggctgg cggcattgct ggtttttaag gtttggtatc cttatcctta
     1981 tgccgagctg acgggagggc tgtcgcttta tcagctggtg gtggctgtcg atattgtatg
     2041 tggtccgctg ctgactttaa ttttggcaag cccgaagaaa aagacaaagg cacgcatggt
     2101 cgatttttcc atggtcggca tcatccagct ggcggctttg gtgtacggtc tgcacagcgt
     2161 ttctttggcg cgtcccgtgg tggaggcgtt tgaaaaagac aggattaatg ttgtgaccgc
     2221 cgccgaagtg gcggttgaag acctgcacaa agcgccggaa gggctgcaaa gcctgtcgtg
     2281 gttcggcatc cgccgcattg cattgaaaga acctgaggat gcggatgaga agaacaagac
     2341 gctggatttg tccctgaaag gtatcgagcc gagtatgcgt cccgaccggt ggctgccgta
     2401 ttccgacaag gaagcagaag aaatccgcaa acatctgaaa ccgctgaaag tcttggcgga
     2461 tgcgagaaaa acgacggttg cggacattct gaaacaggca ggtctcgccg aaggggagga
     2521 gctgtattac ctgccgttta ccagcagcag gcagaaagag tggatagtca ttaccgataa
     2581 agagggcaac accaaaggct acgcgccgat agacggcttc atcatcaccc cttaagcgtt
     2641 gggactccgt ccgcactcga acatccgttc ttcgcggcgg tagaatcaga ctgtatttga
     2701 gaggggcgca acccctcaat cagggcaaca cataggggaa ggctttatgt gccgtccgtg
     2761 tgttgaaaca tttcaaaata agctcatgcc gtccgaacat cctttcagac ggtatggcat
     2821 tttgccattg ccccgataag ctgttaaact atttaaatta tttcgaccga aggtacacgc
     2881 acatgaaaga ccccgagcag agcagcaagc ccgcccgccg ttttttgtgc gtaccgtctg
     2941 ttcaggcagc ttgagcgctt caggctgcct tttcgcgttt gaacttgagg aatacgaaaa
     3001 tcatggattt cagttggttg gcagaaccgc atacctggat aggttttgcc acgcttttgg
     3061 tgttggaagt cgtattgggg atagacaacc ttgtctttgt ggcgattttg gcaaacaagg
     3121 tccagcccgc acggcgcgac cgcgcacgga ttatcgggct ggggctggca gtcgtcatcc
     3181 gcatcattat gcttgctttt atggcgcaca tcatcacgct gaccgagccg ctgttccaaa
     3241 tcggcggcct cgccgtttcc ggcaaggaca tgattatgct cgcgggcggt attttcctgc
     3301 tttacaaagc caccaccgaa ctgcatgaac gcctcgaagg gcacaaccgt tttaccgttg
     3361 ccgacaacca aaaaaaacac gcgccgtttt ggggcgtggt cgcgcaaatc ctgatactgg
     3421 atgccgtgtt ttccatcgat tcggtcatta ctgcggtggc gatggtcgat catatcgtcg
     3481 tggcgatggg tgcggtcgtc gtcgcaatgg ctgtaatgat ttctgccagc aaactcttga
     3541 ccgaatttgt cgacagacac cctaccgtcg tgatgctctg ccttggtttt ttgttgatga
     3601 tcggtttcag cctgattgcc gaagccttcc atttccacat tcccaaaggc tacctctacg
     3661 ccgccatcgg cttctcgatt ttaatcgaac tgtttaacca gatttcccag cgcaacagcc
     3721 gcaaaaacga ctacatcggc agctcgtggc gcaagcgcac cgccgaaaac gttttgggta
     3781 tgatgggcat acgcgaaagc gtgcttgccg atgcgggcgg cgaatccggg gacgacgcgc
     3841 attttgaaga aaacgaaaaa tcgatgatac gcagcgtgct gacgcttgcc gaacgcccga
     3901 ttatgggggt gatgatccca cgccgcgaca tcgaacggct ggacatttcc caaagccgcg
     3961 aagaacagtg tgcccaactg caaaacacgc cttacagccg cctgctcgtt gtcggtaagg
     4021 cgggcgtgga cgaacctttg ggctacatca acaaaaaaga cctgctgtcc caactgctgg
     4081 aaacaggcgg tctcgacatt cagacggcat tgcgccagcc actcgtcctg cccgacagca
     4141 ccaccgcgct gggcgcaatc gaactcttcc gccaaagcag cgcggattat gctttggtgg
     4201 tggacgagtt cggcgcggta ttgggcatgg taaccatgaa agacctgctc gaaaccatcg
     4261 caggcgagtt ccccgaagaa tttgagcgcg aagaagaacc agccgttcag gggaatcccg
     4321 atgaaagcct gacggtggaa ggcgcgttgg aatatgtgga actcgcaccg caactcaacc
     4381 tgccgcagca ggaggaagat gccgatttcc atacggttgc cgggctgatt atggaagaat
     4441 tgcaaaccat ccccgatgtc ggcgattttg ccgatttcca cggctggcgg tttgaagtgg
     4501 tcgaaaaaga agggcagcgc atcgagcggg tcaaaatcac caaattgccc gaagaataag
     4561 tattcaggat agaaaatgaa cgttttgatt tccaacgacg acggctacct ctccgaaggc
     4621 atcgccgttt tggcgcgcgt tacggcggaa tttgccaacg tcagggtggt cgcgcccgaa
     4681 cgcgacagga gcggggtcag caattcgctg acactggaac gccctttgca gttgaaacag
     4741 gcgcaaaacg gcttttacta tgtcaacggc acgccgaccg actgtatcca catcgggcag
     4801 tctgtatttt cggattttca ggccgatttt gtcttttcgg gcatcaaccg gggcgcgaat
     4861 atgggggacg acacgcttta ttcggggacg gttgcggcgg caaccgaagc ctaccttatg
     4921 ggcatacccg ctgtggcgtt ttccttaaac gacgcttccg gacgctattg ggcaaccgca
     4981 gaaaaggcac tgtggacatt gttggcgcat tttttcaaaa accccccgca gtcccctatt
     5041 ttgtggaaca tcaatatccc cgccgttgcg ccggaagatg tgcggggcat taaaatcgcc
     5101 cgtttgggca ggaggcatca cggtcagaac gtcatccccg cgcgcaatcc gcgcggcgaa
     5161 cagatttatt ggataggacc ggtcggcgaa gtttccgatc gggaagaggg aacggatttc
     5221 ggtgaatgcg gcgcaggttt cattaccgta acgccgctgc aaatcgacct gaccgcctat
     5281 ccggacatgg cggaaacagc ggcgttctgg catgcggact gaccgtttca t
//
LOCUS       DAXHNE010000134         5254 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.134, whole genome shotgun sequence.
ACCESSION   DAXHNE010000134 DAXHNE010000000
VERSION     DAXHNE010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.134"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(103..2934)
                     /gene="lbpA"
                     /locus_tag="ACFM4Z_001709"
                     /gene_synonym="tbpA"
     CDS             complement(103..2934)
                     /gene="lbpA"
                     /locus_tag="ACFM4Z_001709"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HFC6021708.1"
                     /translation="MNRRHTFPLTLTALAVAAAFPSYAANPETAAPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTYDIKRKTREPFFSVEGERESK
                     PVAKLAGYGKYLNNQLNRWVKERIEQNQPLSAEEEAQVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAEKFR
                     DKSGVYDGNDFRDGLYFVPNIEEWKGDKKLVRGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASNAISRPEQLSHNAARISESTG
                     FDEKNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSLSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADH
                     KTKLPNQAGQLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(2931..5102)
                     /locus_tag="ACFM4Z_001710"
     CDS             complement(2931..5102)
                     /locus_tag="ACFM4Z_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6021709.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTPPPAGPSVETTPVNRPAVGAAMRLPRRNTAFHREDGTVIPDSKQAEEQLSFKEG
                     DVLFLYGSKENKLQQLKSEIHKRDPDVEIRTSENENKKYDYKFVDAGYVYVKGKDEIK
                     WTSDHKQFSNRLGYDGFVYYSGERPSQSLPSAGTVEYFGNWQYMTDVKRHRAGKAVGI
                     DNLGYITFYGNDVGATSYAAKDVDEREKHPAKYTVDFDKKILKGELIKNQYVRDRNGP
                     YNPLTIYDITATLDGNRFTGSAKVNTKLKTSHADKEHLFFHDDADQRLEGGFFGDNGE
                     ELAGRFISNDKSVFGVFAGKQKTETANASDTNPALPSGKHTKILDSLKISVDEATDSN
                     ARKFAISSMPDFGHPDKLLVEGREISLVKDTQTIDLADGRKMTVSACCDFLTYVKLGR
                     IKTARPASKPKAEDENSEDEIGESEENEEDLVAEEENTEDEVVEDEDREEDEVSEDGN
                     SEDEEEIAEEDDDEAEEEEVEETEEESPVEESGGDSGSILPTPEAPKGRDIDLFLKGI
                     RTAEADIPKTGTAHYTGTWEARIGVPDKKDDQLYGTTFIQKDSYANQGAKAQFTVDFG
                     KKSISGTLTEKNDTHPAVYIEKGVIDGNGFHATARTRDNGIDLSGQGSTNPQRFEANN
                     LLVTGGFYGPQAAELGGNIIDSDRKFGAVFGAKKDDKEATR"
BASE COUNT         1131 a         1404 c         1225 g         1494 t
ORIGIN      
        1 ggaaatcttg tttatatcga atcaaaaaaa cctgcacctt aatcagttgg cggtttagtc
       61 cgacttttgg ggtgcagatc aagctttcag acggtatttc ctttaaaact tcatttcgag
      121 cgcgaggctg aagttcctgc ctggtgcggc ataccttcca tagttgctgt cgccgccgtg
      181 ccggtttgcc gtgctttccg cggtctggcg caaggattcc caagtaacgt agcggtagtt
      241 gccgatattg tagatagccg ccctcaaggt cagccgtttt ttcagattca gataggcgga
      301 aacgtctgcc gtcgaccaag aagacgacgc tctttttgtc gaatatcgtt tttgatcgcc
      361 tgccagataa gcaagctcgt cagggttttt ccctttggaa taggtcagca taatgtttgc
      421 gccccatttc ccctctggct ggtcgtatcc gaaccccaaa acataacgcg acggctgtac
      481 cgcatccaaa gcatagctgc ggagggacag tcccggccgg ttggataccg atttcggttt
      541 gatgcggttg tacgccaaag tcgtgtacaa accttcgggc agtttgccat acacgccgtt
      601 ccagtcgatt tttcccaata tattaacgcc ttgaagcgac atattttggg cattgtaata
      661 atcgcgtata tcaatctctg tcaattgtcc tgcctgattc ggcaatttgg ttttgtgatc
      721 ggcaacggca atcatatcgg tataacggtt gcggaagctg ctgatttcca aaaagccgaa
      781 atcgcccttc cactgcaaac cgatttcgcg gttggctgcc ttttccgatt tcagggcggg
      841 acgctgccag cctttcggat aatcgtgata aatgtctatc ccgaaaagtt cttggaatga
      901 gggcgttctg aagccgctgg aggcgcggta agacaaagaa aaatgccggt tcggtttgaa
      961 cacgatgccg ctgttccacg aacggtcaac ataccgcccg ctgcggacga gttcttccga
     1021 cgtggtgaag tttttccggt catacctgcc acccaagctg aaatcaaaat atttgccgat
     1081 tgaaaaacgg tcgttcaaag aaatatggat attgctgccg ttgatttttc ttggcacgca
     1141 tttgcgggaa cgcagggttt cgatgtagcc gcagaccgac ccttcgacga cttcgggctt
     1201 acccaaacgg tacttatctt gattcttttc atcgaatccc gtggattccg aaatccttgc
     1261 cgcattgtgg gaaagctgtt cagggcggga gatcgcgttg gaagcatcgt aaccgaagcc
     1321 caaagtcaga tggtgtttcg tccatttgtt tttcagcgac ttctcaaacg aggcattcaa
     1381 aacattgtgc tgttcgcggt agtggaaacg gtcgctgctg tcgtaggaat acggtttgtc
     1441 cgccgacgcg cggcaggatt tgtccacagc aggatacacg gcacaattca gcttcagcgt
     1501 gttgttgtcg gttgccacgc cctgtttgtc aaacgacaac accgccttgt ccgcccaatt
     1561 gtcagaatac gcttcgtttt cataacggta cagcaaaccc atacggcggc ggcggtgatg
     1621 ttcgtcaata aatttggtgc gggaatattt caaacctatg cccctgacca actttttatc
     1681 gcccttccac tcttctatat tcggcacaaa atacaagccg tcgcggaaat cgttgccgtc
     1741 gtacaccccg ctcttgtctc taaacttttc cgcctcgtcc gtaccgtaat actgtttttc
     1801 cgtcatatcg cggatgtcgt aacgctgttt ggtatcctca aacacaccgc cgacataatg
     1861 cctgccgccg aagcggtagc ccagcttggc aagccacgaa ccgctgcggt aatccatcgg
     1921 atcgggcaat atcctgccgc cgcccgtgta agcttgggcg gacagatttt cgtggcgcgc
     1981 ctgcgcctcc cgcacctgcg cctcttcttc agcacttaaa ggctgatttt gttcaatacg
     2041 ttcttttacc cagcggttga gctggttgtt caaatatttc ccgtagcccg ccaattttgc
     2101 cacgggcttg gattcacgct cgccctctac tgagaaaaat ggctctcttg tcttgcgttt
     2161 aatatcgtat gtctggcgga acgcgtccaa acggtttatg ccgtatgcca ccccgtccgc
     2221 aatatcgccg tgcgggcgcg tttcccgccc ttggcgttcg gtacggatta gcagcccttc
     2281 ccaaccgtct ttgctgaacc ccgcgccgag cgacttcata aattggcggt ttttactgcc
     2341 gtaggcggtt tttgcctgta tcccccagct tttgccgtct gaaatcaggt ctgccgcctc
     2401 tttggtgcgg aaggcgaccg cgccgccgag tgcgccgctg ccgtgatcgg acgaaccggc
     2461 acctttgtcg atttccaccg tgctgatgtt ttcatattcg atttcgttga ttgcaccgct
     2521 gccgccgcgt ccgccgtatc cgctcaacga tccctgcacg gtaaacgcct gtatttgggc
     2581 aacgccgtca accgaaaccg ccacacggtt tttatccacg ccgcgtatcg agtagccgcc
     2641 gctcgcgccg ttgccctgtt cgacaaccgc cacgcccgga tcgtagcgcg tcaggtcgcg
     2701 gataccgagt acctgttctt tgttcaacgt ttccgacgtt ttgacgattt tgcccaaacc
     2761 ggtcgcctct ttcgatcgcc gtcccacttt ggcggcacgg acggtaacct ctttcaggga
     2821 ttgggtctgc gccgcatcag gtgccgccgt ttcagggttt gccgcatatg acgggaaggc
     2881 ggcggcaaca gccaaggcag tcagggtaag tgggaatgta tgccttctgt tcatcgtgtt
     2941 gcctccttgt catctttctt cgcaccgaat accgcgccga atttccggtc gctgtcgata
     3001 atattaccgc ccaattccgc cgcctgcggt ccataaaagc cgcctgttac aagaagattg
     3061 ttggcttcga agcgctgcgg gttggtcgaa ccctgcccgg aaaggtcaat accgttatcc
     3121 cgagtgcgtg ctgtcgcgtg gaaaccgttg ccatcaatca cacctttttc aatataaaca
     3181 gcggggtgtg tatcattttt ttctgtcaac gttccggaaa tcgatttctt gccgaaatca
     3241 acggtaaatt gtgcttttgc cccttgattt gcatagctat ccttttgaat gaacgtagtg
     3301 ccatataact gatcgtcttt cttatccggt acaccgatac gcgcttccca agtgccggta
     3361 taatgcgccg ttccggtttt tggaatgtcg gcttccgccg tgcggatacc tttcaggaaa
     3421 aggtcgatgt ccctgccttt aggggcttcc ggagtgggca ggatgctgcc tgaatcgcca
     3481 ccgctttctt ccaccggcga ttcttcttcg gtttcttcaa cttcctcctc ttcggcttca
     3541 tcatcatctt cttcggcgat ttcttcttcg tcttcactgt taccatcttc ggaaacttcg
     3601 tcttcttctc tgtcttcatc ttctacgact tcgtcttccg tgttttcttc ttcagcgacc
     3661 aaatcctctt cattttcctc gctttcacca atttcatctt cggaattttc atcttccgcc
     3721 tttggtttac ttgccgggcg ggcggttttt atccgtccga gtttcacata ggtcaaaaag
     3781 tcgcaacaag cactgacggt cattttcctg ccgtcggcaa gatcaatggt ttgagtatct
     3841 ttgaccaagg agatttcacg cccttcgaca agaagcttgt cgggatgacc aaaatcgggc
     3901 atagaggaaa tggcaaactt acgggcattg ctatcagtcg cctcgtcaac ggaaattttt
     3961 agagaatcca agattttggt gtgttttcca gacggcaggg caggatttgt atctgatgcg
     4021 tttgctgtct ctgttttttg tttgcctgcg aatacgccga atacgctttt gtcgttgctg
     4081 ataaaccgtc cggcaagctc ttctccgtta tcaccgaaaa aaccgccctc aagccgctga
     4141 tcggcatcgt catggaaaaa caaatgctct ttatcagcgt ggctcgtctt caacttggtg
     4201 ttaactttgg cactgccggt aaagcggttg ccgtccaatg ttgcagtaat gtcgtaaatg
     4261 gtcagcggat tatagggacc atttctatct cgcacatact gatttttaat cagctcgccc
     4321 tttaggattt ttttgtcgaa atcaaccgta tatttggcag gatgcttttc cctttcgtcg
     4381 acatccttag ccgcataaga agttgcacca acatcgttac cataaaatgt gatataaccc
     4441 aaattgtcaa tgccaaccgc cttacctgct cgatgacgtt tgacatcggt catatattgc
     4501 cagttaccaa aatattccac cgttcccgca ctcggtaaag attgggaagg acgttctccg
     4561 gaataatata caaaaccgtc ataacctaag cggttggaaa actgcttgtg atctgaagtc
     4621 cacttaattt catcttttcc ctttacatat acataacctg catctacaaa tttataatca
     4681 tattttttat tttcattttc tgatgtccta atttctacat cgggatcacg tttatgaatt
     4741 tcgcttttaa gttgttgaag tttattttct tttgaaccgt ataaaaacag aacatcacct
     4801 tctttaaacg acagctgttc ttctgcttgt ttgctatcgg gaattaccgt gccatcttca
     4861 cgatgaaaag cagtattccg ccttggcagc cgcattgccg caccgacggc gggccggttg
     4921 accggcgtgg tttctaccga aggcccggca ggggggggag tgggaacgtc cttagacttg
     4981 aaagtgacgg ggtacgcggt cggcgttgat tcgacaacag gctgcacgcc gaaattgccg
     5041 ccgatacaag atgctaaaag taagggcaac aagacaatgc cgccataatt cggtttacac
     5101 atccctactt ttcctcctat ttgattaata ataattatca ttatattaat atgtacagat
     5161 aatatcaagc cgtttttata gtgaattaac aaaaatccgg acaaggcgac gagccgcaga
     5221 cagtacagat acattccgtc attcccacga acct
//
LOCUS       DAXHNE010000135         5233 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.135, whole genome shotgun sequence.
ACCESSION   DAXHNE010000135 DAXHNE010000000
VERSION     DAXHNE010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.135"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            86..1561
                     /gene="trpE"
                     /locus_tag="ACFM4Z_001711"
     CDS             86..1561
                     /gene="trpE"
                     /locus_tag="ACFM4Z_001711"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFC6021710.1"
                     /translation="MISKQEYQAQAAQDYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKAGGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSNPESEWQETQNKARAVIHAAQMVQEGLDK"
     gene            1558..2103
                     /locus_tag="ACFM4Z_001712"
     CDS             1558..2103
                     /locus_tag="ACFM4Z_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFC6021711.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKNREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRGAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            2100..3236
                     /locus_tag="ACFM4Z_001713"
     CDS             2100..3236
                     /locus_tag="ACFM4Z_001713"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFC6021712.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDA
                     PAAEFADCHLCAPFNDQAALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPGSDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQVVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHGGVDCVLEKMVDLRSEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADVQQQARQMAQRLADELDYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTIDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGKPDWFPLQS
                     RPDARLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            3283..3765
                     /locus_tag="ACFM4Z_001714"
     CDS             3283..3765
                     /locus_tag="ACFM4Z_001714"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6021713.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            3871..4926
                     /locus_tag="ACFM4Z_001715"
     CDS             3871..4926
                     /locus_tag="ACFM4Z_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6021714.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALLDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISTESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDSFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            4932..5216
                     /locus_tag="ACFM4Z_001716"
     CDS             4932..5216
                     /locus_tag="ACFM4Z_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021715.1"
                     /translation="MPSEPPFRRHFTHQNRKTAPKIIITGRNGKPDTKPNTAAKMPSE
                     AVGATILIWGPLQGRITFLNHPFDSQINSAFACQKQNQYFAVRIGTFIVD"
BASE COUNT         1494 a         1593 c         1115 g         1031 t
ORIGIN      
        1 cggttagact gtcaacaatt ccgataaaat gctatactcg cgcccattgc cgcgccgtcc
       61 gaaaccgccc tttgggaaag cagaaatgat cagcaaacaa gaataccaag cccaagccgc
      121 ccaagactac aaccgcatcc cgctcgtgca agaacttctt gccgacttgg atacgccgct
      181 ttccctctat ctcaaactcg ccaaccgccc ctatacctac ctgctcgaat ccgttgtcgg
      241 tggcgaacgt ttcggccgct attcctttat cggcctgcct tgcagccact atctcaaagc
      301 cggcggcaaa cacgtcgatg tttatcaaaa cggcgaaatt gtcgaacaac acgacggcaa
      361 tccgctgccc tttatcgaag ccttccacaa ccgcttcaaa acacccgaaa tcccaagcct
      421 gccgcgcttt accggcggac tggtcggcta cttcggttac gaaaccatct acaattttga
      481 acacttcgcc caccgcctga aaaacaccac caaagccgac ccgctcggca cgcccgacat
      541 cttgctgatg ctgtcgcaag aattggcggt gatcgacaat ttgagcggca aaatccatct
      601 cgtcgtttac gccgatccgt cgcagcccga cggctacgaa cgcgcccgcg aacgcctcga
      661 agacatccgc acccagttgc gccaaagctg cgccatcccg ctctcgctcg gcagcaaaca
      721 caccgaagcc gtcagcgaat tcggcgaaga gccgttcaaa gcctgcgtta acaaaatcaa
      781 agactacatc ttcgcaggcg actgcatgca ggttgtcccc agccagcgca tgagcatgga
      841 attcaccgac agcccgctcg ccctctaccg cgccttgcgc acgcttaacc cttcgcctta
      901 tctcttttac tacgatttcg gcgatttcca catcgtcggc tcctcgcccg aaatccttgt
      961 ccgccgcgaa cgcgacgatg tcatcgtccg ccccatcgcc ggcacgcgcc tgcgcggcaa
     1021 aacccccgcc gaagaccttg ccaacgaaca agatttgtta agcgatgcca aagaaatcgc
     1081 cgaacacgtc atgttgattg acttaggacg caacgacgtc ggccgcatca gtaaaaccgg
     1141 cgaagtcaaa gttaccgaca aaatggtgat tgaaaaatac tcccatgtaa tgcacatcgt
     1201 ttccaacgtc gaaggccgtc tgaaagacgg catgaccaac atggacatcc tcgccgccac
     1261 gttccctgcc ggcacacttt ccggcgcacc caaagtccgc gccatggaaa tcatcgaaga
     1321 agtcgaacca agcaaacgcg gcatctacgg cggcgccgtc ggcgtatggg gtttcaacaa
     1381 cgacatggat ttggcaatcg ccatccgcac cgccgtagta aaaaacaaca cgctatacgt
     1441 ccaaagcggc gcaggcgtag ttgccgattc caatcctgaa tcagaatggc aggaaacgca
     1501 gaacaaagcc cgtgccgtca tacacgccgc gcaaatggta caggaagggt tggataaatg
     1561 acttatgaaa aactttatga gcttcgccaa acactcagac cgactaccaa agacggtctc
     1621 tatacagata atgaaaaaaa tcgagaaatc ctaacggtta gatggagcgg caacacagaa
     1681 aatcctaaaa atttctcagc tgtcgctata ggaattaatc caagtaaagc aaatgatgaa
     1741 agaagcgaca aaacccttac ccaacttgca cgtttcttgg atatgtacgg ttttaccaac
     1801 ttcaaaatgt taaatatttt ttccagttac tctacacagc aaacaggaat tagggcaaac
     1861 acccaaaccg atttttccaa atttaaagga tgtttggaag atgctgatat gattattctt
     1921 gcatggggta cagaccgcgg tgcttataag gatgaaaaaa acagaatact ggaatttcta
     1981 aaagcagaaa aatttatgga aaaagtattc tgtatttctg aaactggtaa ttccagcgat
     2041 acaagacatc cgtcaagaat tagttatagc tatcaacttg tacaatttga agagtcagca
     2101 tgaaaaacat atctctttct ccgcccgcca tgcttggcat cctcggcggc ggacaattag
     2161 gcagaatgtt taccgttgcc gccaaaacca tgggctacaa agtaaccgtt ctcgaccccg
     2221 atccggacgc gccggcagca gaatttgccg actgtcattt atgcgcgccg tttaacgacc
     2281 aagctgcttt ggacgaattg gcaaaatgcg cggcggtgac cactgaattt gaaaacgtca
     2341 atgccgatgc gatgcgtttt ctcgccaaac acaccaatgt ttcccccggc agcgactgcg
     2401 ttgccatcgc gcaaaaccgc attcaggaaa aggcatggat acgcaaagca ggattgcaaa
     2461 ccgcgccgta tcaagtggtt tgtaaggctg aagacattac tgaagcaagt gcgcaattct
     2521 tgcccggcat cctgaaaacg gccactttgg gctacgacgg caaaggtcaa atccgcgtca
     2581 aaacggtgga tgaactcaaa gctgcgtttg ccgaacacgg cggcgtggat tgcgttttgg
     2641 aaaaaatggt ggatttgcgc agtgaaattt ccgtaatcgt atgccgtttg aacaatgaca
     2701 acgtgcaaac tttcgaccct gccgaaaaca tccacgaaaa cggcatcttg gcttattcca
     2761 tcgtccccgc gcgactgagt gccgacgtgc agcaacaggc gcggcagatg gcgcaacgct
     2821 tggcggacga attggattat gtcggcgtat tggcggtaga aatgtttgtt gtcggtgaca
     2881 cacatgaatt ggtcgtcaac gaaatcgcgc cgcgtccgca caactccggc caccatacca
     2941 tcgatgcctg cgcggcagac caattccagc aacaggtccg cctgatgtgc aacctgccac
     3001 ctgccgatac caaattgctg agttcttgct gtatggcgaa tattttgggc gacgtttggc
     3061 aggaagacgg cggcaaaccg gattggtttc ccctgcaaag ccggccggac gcgcgcctgc
     3121 acctttacgg caaaaaaacc gcgcacaaag ggcggaaaat gggacacttt accgttttaa
     3181 ccaccgattc ggacaccgca tttcaagaag caaaaaaact gcatcagtcc ctataatccg
     3241 accaacggca cagatgccgt ctgaagccgc cgacatactg ccatgaacct gctgacactg
     3301 ttacgccccg ccaccgtgca agactgcaaa gacatcttca aagtccacct gcactccgtc
     3361 caatacacct gcatcctcag ctacaacgaa cacgccctaa aagtatggga aggcttgtta
     3421 aacacagaaa gctacctgcc gaccatttcc gatcccgaca aagcactttg ggttgccgaa
     3481 tacaaaggca acatacaggg atttttccaa atcgactgcc aagaagcaca actcgatgca
     3541 ctttacgtcc accccctgtt ccacaacctc ggactgggca ctgccctact tcatcaggcg
     3601 gaaaccatcg cccacaaatc cggtttgagc tttctgaaac tctacgcatc gctcaactcc
     3661 gtcccgtttt accttctcaa ccgctacgaa tcgctcggtt cggcagtatt gcagcttgac
     3721 cccagcatca aaatcaaatg cgaactgatg cggaaacacc tctgatccgt ccttataacc
     3781 aaaactgata atcttcaaat caaacattat ttccacaagc gcagacagcc gtataaagtt
     3841 gccccatcta ccaaccgaca ggacaagcat atgaaaacct acgcaccggc actctatacc
     3901 gcagccctgc tcaccgcctg cagccccgca gccgattcaa accatccgtc cggacaaaat
     3961 gccccggcca ataccgaatc cgacggaaaa aacattaccc tgctcaacgc ctcatacgat
     4021 gtggcacggg atttttacaa agaatacaac cccttattta tcaaaacata ccaatccgaa
     4081 caccccggca catccgtcag catccaacag tcccacggcg gctccagcaa acaggcatta
     4141 tccgtagcca acggccttca agccgatgtc gtaaccatga accaatcctc cgacatcgac
     4201 ctgctcgaaa aaaaaggact ggtagaaaaa ggctggcaac aagccctcct cgaccacgcc
     4261 gcgccctaca ccagcactat ggttttcctt gtccgaaaaa acaaccccaa acagatccgc
     4321 gattggaacg accttgccaa agacggcgtt aacatcgtca tcgccaatcc caaaacctcg
     4381 ggcaacggac gctacgcctt cctcggcgca tacggttacg gtctgaaaac caccaacggc
     4441 aacgaacagg aagcccaaaa actcgtcgca tccatcctca aaaacacccc cgtttttgaa
     4501 aacggcggac gcgccgccac caccaccttc acacaacgca acatcggcga cgtactcatc
     4561 acttttgaaa acgaagccaa ctacgtcagc aaaaaactga cccaaggaca gtttgaaatc
     4621 gtctatccga gctacaccat ttccaccgaa agccccgtcg ccgtcgtcaa cagcgtcgtc
     4681 gcgaaaaaag gcacgcaaaa aaccgcccgc gcctatctcg aatacctttg gagcgaaccg
     4741 gcgcaagaac tcgccgcctc actctacctg cgcccccgca accccgaagt attggcaaga
     4801 cacaaagccg acttcccaga cttagactca ttctctcccg aagaaaaatt cggcggatgg
     4861 gacaacatca tgaaaaccta cttcgccgac ggcggcatat tcgaccgcct gaccgcacaa
     4921 aaatgaccgc tatgccgtct gaaccgcctt tcagacggca tttcacacat caaaacagaa
     4981 aaaccgcgcc caaaatcatc atcacaggac gcaacggcaa accggataca aaacccaaca
     5041 ccgccgccaa aatgccgtct gaagccgtcg gcgcaaccat ccttatctgg ggcccgcttc
     5101 aggggcgcat aactttcctt aaccatcctt ttgacagcca aataaacagt gcttttgcct
     5161 gtcagaaaca aaaccaatat tttgcggttc gtatcggaac atttatagtg gattaacaaa
     5221 aaccagtaca gcg
//
LOCUS       DAXHNE010000136         5163 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.136, whole genome shotgun sequence.
ACCESSION   DAXHNE010000136 DAXHNE010000000
VERSION     DAXHNE010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.136"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(31..447)
                     /gene="dksA"
                     /locus_tag="ACFM4Z_001717"
     CDS             complement(31..447)
                     /gene="dksA"
                     /locus_tag="ACFM4Z_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFC6021716.1"
                     /translation="MAKLTEQDILNWSGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(594..1403)
                     /gene="proC"
                     /locus_tag="ACFM4Z_001718"
     CDS             complement(594..1403)
                     /gene="proC"
                     /locus_tag="ACFM4Z_001718"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFC6021717.1"
                     /translation="MNRVEIMNVYFLGGGNMAAAVAGGLVKQGGYRIYIANRGAEKRE
                     RLEKELGVETSATLPELHSDDVLILAVKPQDMEAACKNICTNGALVLSVAAGLSVDTL
                     SRYLGGSRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDCKIADRIMKSVGLTVWLEDE
                     VQMHSITGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGE
                     DFEKLQKNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1434..1842)
                     /locus_tag="ACFM4Z_001719"
                     /pseudo
     CDS             complement(1434..1842)
                     /locus_tag="ACFM4Z_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            complement(1842..2528)
                     /locus_tag="ACFM4Z_001720"
     CDS             complement(1842..2528)
                     /locus_tag="ACFM4Z_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFC6021718.1"
                     /translation="MTVLQERYCEVSDRIGKLVLQAGRKPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSEQRPSSMPPLQVCIEVNIAGEAVKHGVAPEEAVALAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLADLNAAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2648..3691
                     /gene="pilT"
                     /locus_tag="ACFM4Z_001721"
     CDS             2648..3691
                     /gene="pilT"
                     /locus_tag="ACFM4Z_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFC6021719.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNIGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3854..5080
                     /locus_tag="ACFM4Z_001722"
     CDS             3854..5080
                     /locus_tag="ACFM4Z_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC6021720.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
BASE COUNT         1288 a         1611 c         1161 g         1103 t
ORIGIN      
        1 gggactcaga cggcattttg tttgccgcca tcagtcggca aactgttttt tcatcctctc
       61 tcggcgttct tgggactcaa cagataaagt ggctgtcggg cgtgccagca gccgcttcag
      121 accgataggc tctcccgtat cggcacagaa tccataatcc ccttcatcaa tattgcggat
      181 ggtcgcctgt attttactga gaagttttcg ttcccgatcg cgggtacgga gttccaatgc
      241 gtactcttct tcctgtgtgg cacggtcggc aggatcgggg gctgattcgt gttcttggag
      301 atgccctgtc gtagcggaag cattttcgat gagttcgtct tgcattttta ccagcaattc
      361 gcggaaaaaa gccaaatggt cgtcattcat ataatcgtct tccggcccgc tccaattcaa
      421 aatatcttgt tctgtcagct ttgccataat gtatatcctc tcggttcacc gaaaaaaaca
      481 aagcaccaca aagtggcttt attcctttga tttttataag gcttattgcc taaggcgttt
      541 ttgaaaacgc gcatcataac acgttttgga ttggtttttt tattttcttt acattattga
      601 tattgccgtt ccatttcctg cgaacggcgc acacaggcac aaacgccctc acttatggct
      661 tcggcgacac gatgccgcct gaaggcttcc acggcttcgt gggttgtccc gccttttgac
      721 gttacatttt tttgaagctt ctcgaaatct tcacccgtct gctcggcaag ggcaaccgct
      781 cctttaaacg ttgccagact gagcgcgcgt gcttctgcca tatcaaaccc ttgtcggatg
      841 gcggcatttt gcaaagcatc cagcagataa aacacataag ccggcccgct gccgctgatg
      901 ccggtgatgc tgtgcatttg cacttcatct tccaaccaaa cggtcaaacc gacagatttc
      961 atgattcgat cggcaatctt gcagtctgtt tccgatactt ccgcttcggc atacataccg
     1021 gagacaccca gtcctatttt cccgggtgta ttcggcataa cccggacaat gcggcggctt
     1081 cccccgaggt aacggctgag cgtatcaacc gacaatccag ctgcaacaga aagcaccaat
     1141 gcgccgttgg tgcagatatt tttgcacgcg gcttccatat cctgcggttt gacggcaagg
     1201 attaaaacat cgtcggaatg aagctccggc agggttgccg aagtttcgac ccccaactct
     1261 ttttccaaac gttcgcgttt ttccgcaccc cgattggcta tatagatgcg gtaaccgcct
     1321 tgtttgacca atccgcccgc aacggcagcc gccatattgc cgccgccgag aaaataaaca
     1381 ttcattattt ctaccctatt caaaattacc tgactaaatt cattcccaat tttttaccat
     1441 cggacgcgct cggacagacc gttgcgccgc aacccctgct tttccaagca gtcaaatcct
     1501 tcctgtttga acgtctcaat caaaggtttg ccgtcacgcg tacgccaatc caacccgcag
     1561 gcttcaaact tccctatttt cctgtaaaag taagattgtt gtgcggattc atattcgttt
     1621 gccctgacgg aacgattgcc gtctgaaagc ggtatctcgt agtcctctaa agacggcgga
     1681 ctctcgccgc caatatccca aaaatcgatg gcagccggtt ttctcggctt acaccagccg
     1741 gacagggacg aacactcaat accaacccgc caaccggcag cctgcaaccg taaatgcaag
     1801 caacgcagcc attgccgtat attttattgc ccgaatttcc atcagcccct tttcccgaaa
     1861 atcgcgctgc cgatacggac gtgtgtcgca ccgcactcaa tggcggcagg catatcgtcc
     1921 gacatcccca tagacagcac gtctgcctta acgccagccg cattgaggtc ggcaagcagt
     1981 ttccgcatcg tttgaaattg caccttcaac tccgtttcac tgctgttggc tttggcaaca
     2041 cacatcagtc cgcgtacgac gatattcggc agcttcgcca cttccacagc aagcgcgact
     2101 gcttcttcgg gcgcgacacc gtgcttcacc gcctcgcccg caatattcac ctcgatacac
     2161 acctgcaagg gcggcattga ggaaggacgc tgttcgctca accggacagc ggttttcaga
     2221 cggcatacgg tatgcaccca atgcgcgcgt tcggcgacaa atttggtttt attggactgc
     2281 acatcgccga tgacgtgcca cacgatgtcg gtcaaatccg ccaactcttc cgttttgccg
     2341 taccactcct gaatatagtt ctcgccgaaa tcacgctgtc cggcggcgta aacttcgcgg
     2401 atgccgtctg aagggaaagt cttaccgacg gcaatcaggc tgacggaatg cggcttcctt
     2461 cccgcctgca gaaccaattt tccgatacgg tcggacacct cacaataacg ttcttgcaac
     2521 accgtcatag attatcccct aattaaaaat gattaaacag ttgaaacccc tccagtcagg
     2581 gcggtacaat caaggttgtt agaaccattc caaccaatcg aaacattata ctaaacagag
     2641 ccgcattatg cagattaccg acttactcgc cttcggcgct aaaaacaaag catccgacct
     2701 tcacctgagt tcgggcatat cccctatgat tcgggttcac ggcgatatgc ggcgcatcaa
     2761 ccttcccgaa atgagcgcgg aagaggtcgg taatatggta acttcggtga tgaacgacca
     2821 ccagcggaaa atctaccagc aaaacttgga agtcgacttc tcgttcgaac tgcccaacgt
     2881 cgcccgattc cgcgtcaacg ccttcaacat cggccgcggt cccgccgccg tattccgcac
     2941 cattcccagc accgtcttat cgctggaaga attgaaagcc ccgagcattt tccaaaaaat
     3001 cgcagaatcg ccgcgcggca tggttttggt taccggccct accggttcgg gcaaatcgac
     3061 cacgcttgcc gcaatgatca actacatcaa cgaaacccag ccggcacaca tcctgaccat
     3121 cgaagacccg atcgaattcg tccaccaaag caaaaaatcc ctgattaacc agcgcgagct
     3181 gcaccagcac accctcagct tcgccaacgc gctgcgttcc gcattgcgcg aagaccccga
     3241 cgttatcctt gtcggcgaga tgcgcgaccc agaaaccatc ggcttggcgc tgaccgccgc
     3301 cgaaaccgga cacttggttt tcggcacgct gcacacgacc ggcgcagcaa aaaccgtcga
     3361 ccgtatcgtg gacgtattcc cagcgggaga aaaagaaatg gtgcgctcta tgctgtccga
     3421 atcgctgacc gccgtcatct cccaaaacct gctgaaaacg cacgacggca acggccgtgt
     3481 cgcctcgcac gaaatcctga ttgccaaccc cgccgtccgc aacctcatcc gcgaaaacaa
     3541 aatcacgcag attaactccg tcctacaaac cgggcaggcg agcggtatgc agacaatgga
     3601 ccaatcgctg caatcgctgg tgcgccaagg gctgatcgca ccggaagtcg cacgcagacg
     3661 cgcgcaaaac agcgaaagta tgagtttctg acacacaacc gccttccggc cataccgacg
     3721 ggaaaacaag gcgcaaacac gcggggcggg acgcagcatc ccgcccggct acctttccga
     3781 acaagaagcg tccgccttcc tgttgaaacc tgccgccgca aactgcaagg cttaaaccga
     3841 aaagaagcta acgatgaata ccgataacct gcacgacatc ttggacgaaa tggttcaagt
     3901 gtattctcaa aaaaaacaaa gccgatccga aaccccggcc gaaatcggcg cacacttcca
     3961 cccgctgctc gaccgcctgt gcgaaaccgc agaagcacaa aacgcgtccg acatccttat
     4021 cagcaaagga ttcccgccct cgttgaaaat caacagcgca ttaaccccgc agccgcaaaa
     4081 ggcgctgacg ggcgaggaaa ccgccgccat cgccgcatcg acgatgaacg ccgaacaatc
     4141 ggaaatattc cggcgcgacg gcgaaatcaa ctactccgtc cagtcgcgca gcggcacgcg
     4201 ctaccgcgcc aacgcctacc acagccaagg cagcgcaggt ttggttttgc ggcgcatcaa
     4261 ccacgtcatc ccgcaaatgc aggaattggg cctgcccgaa aaactcaaag acctcgccgt
     4321 cgcaccgcgc gggctgctga ttatcgtcgg gcctaccggt tcgggcaaat ccaccacgat
     4381 ggcgactatg ctcgaacacc gcaacaaaac cctgcccagc catatcgtta ccatcgaaga
     4441 cccgattgag tttatctaca aaccgcgccg ctgcatcttt acccagcgcg aaatcggcgt
     4501 cgacaccata aactggcaga cggcggtaca aaacgctatg cgccaatccc ccgacgtggt
     4561 ctgcatcggc gaagtccgca gcagggaaag tatggaatac gcgatgcagc tcgcccaaac
     4621 cggccacctg tgcattttta cgctccacgc caacaccgcg ccgcagtcgc tcgaacgcat
     4681 actcaacttc taccccaaag aacagcacaa ccaaatactg atcgacatcg ccctcaacct
     4741 gaccggcatc atctgccaac gcctcgccct caaacaagac aaaacgggca ggacggcggt
     4801 tgtcgacttg ctcatcaaca cgcccgccat ccaagacttc atcctgaagg gcgacctgat
     4861 gaacatcagt aaaatcatgg aaaccgccaa aaccgacgga atgcagacga tggatcaaaa
     4921 ccttttcgaa ctgtaccgtc acggcatcat cagttacgaa gaagccctgc gccagtccgt
     4981 ttccgccaac aacctgcgat tgcacatcca actgcacaaa gaaggcaaaa cgcccgaact
     5041 cctttacgac agggtcaacg gtctcaacct catttcctga tccgcaaaac ccaatgccgt
     5101 ctgaaaaccg catccccgtt ttcagacggc atgattttat ccgccccatt catgtgctac
     5161 act
//
LOCUS       DAXHNE010000137         5153 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.137, whole genome shotgun sequence.
ACCESSION   DAXHNE010000137 DAXHNE010000000
VERSION     DAXHNE010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.137"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            150..1691
                     /locus_tag="ACFM4Z_001723"
     CDS             150..1691
                     /locus_tag="ACFM4Z_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6021721.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAGASALVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGGYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETRQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFALGIFLLLDGGEGGK
                     QTETL"
     gene            1720..2319
                     /locus_tag="ACFM4Z_001724"
     CDS             1720..2319
                     /locus_tag="ACFM4Z_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFC6021722.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESQIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            2316..2921
                     /gene="nadD"
                     /locus_tag="ACFM4Z_001725"
     CDS             2316..2921
                     /gene="nadD"
                     /locus_tag="ACFM4Z_001725"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFC6021723.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPTGGPYH
                     KDAASASAADRLAMVELATAEDVRFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRNLASQGVSDGIPPAAARYIREHGLYEK"
     gene            2980..3366
                     /gene="rsfS"
                     /locus_tag="ACFM4Z_001726"
     CDS             2980..3366
                     /gene="rsfS"
                     /locus_tag="ACFM4Z_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFC6021724.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            3420..3890
                     /gene="rlmH"
                     /locus_tag="ACFM4Z_001727"
     CDS             3420..3890
                     /gene="rlmH"
                     /locus_tag="ACFM4Z_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFC6021725.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWQQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE"
     gene            3963..4229
                     /locus_tag="ACFM4Z_001728"
     CDS             3963..4229
                     /locus_tag="ACFM4Z_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFC6021726.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWMAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            4386..5069
                     /locus_tag="ACFM4Z_001729"
     CDS             4386..5069
                     /locus_tag="ACFM4Z_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFC6021727.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVALLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
BASE COUNT         1021 a         1363 c         1560 g         1209 t
ORIGIN      
        1 gcttcgtcgc cttgtcctga tttttgttaa tccactatat ttcagacggc gggaggaagg
       61 cggatacggt atgtagtcgg attgaaatat acggtatgtg ggggcttttg ggtacaatcg
      121 caacatcagt gtttcaagtg gggaaggcga tggatggacg gcgttgggcg gtatggggtg
      181 cttttgccct gctgccttcg gcttttttgg cggtaatggt cgttgcgcct ttgtgggcgg
      241 tggcggcgta tgacggtttg gcgtggcgcg cggtgctgtc ggatgcctat atgctcaaac
      301 gtttggcgtg gacggtattt caggcagcgg caacctgtgt gctggtgctg cctttgggcg
      361 tgcctgtcgc gtgggtgctg gcgcggctgg cgtttccggg gcgggctttg gtgctgcgcc
      421 tgctgatgct gccttttgtg atgcccacgt tggtggcggg cgtgggtgtg ctggctctgt
      481 tcggggcgga cggcctgttg tggcgcggac ggcaggatac gccgtatctg ttgttgtacg
      541 gcaatgtgtt tttcaacctt cctgtgttgg tcagggcggc gtatcagggg tttgtgcaag
      601 tgcctgcggc acggcttcag acggcacgga cgttgggcgc gggggcgtgg cggcggtttt
      661 gggacattga aatgcccgtt ttgcgcccgt ggcttgccgg cggcgtgtgc cttgtcttcc
      721 tgtattgttt ttcggggttc gggctggcat tgctgctggg cggcagccgt tatgccacgg
      781 tcgaagtgga aatttaccag ttggtcatgt tcgaactcga tatggcgggg gcttcggcac
      841 tggtgtggct ggtgttgggg gtaacggcgg cggcagggtt gctgtatgcg tggttcggca
      901 ggcgcgcggt ttcggataag gcggtttcac ccgtgatgcc gtcgccgccg cagtcggtcg
      961 ggggatatgt gctgctggcg tttgcggcgg cggtgttgtc tgtgtgctgc ctgtttcctt
     1021 tgttggcaat tgttgtgaaa gcgtggtcgg ccggcgaatc gtggcgtgtg ttaatggaaa
     1081 gtgaaacgag gcaggcagtg tggaatactt tgcgcttctc ggcggcggcg gtgtttgcgg
     1141 cggcggtttt gggtgtggtg tatgcggcgg cggcgcggcg gtcagcgtgg atgcgcgggc
     1201 tgatgttttt gccgtttatg gtgtcgccgg tttgtgtttc ggcgggcgtg ctgctgcttt
     1261 atccgcagtg gacggcttcg ttgccgctgc tgctggcgat gtatgcgctg ctggcgtatc
     1321 cgtttgtggc aaaagatgtt ttatcagcct gggatgcact gccgccggat tacggcaggg
     1381 cggcggcggg tttgggtgca aacggctttc agacggcatg ccgcatcacg ttccccctct
     1441 tgaaaccggc gttgcggcgc ggtctgactt tggcggcggc gacgtgtgtg ggcgaatttg
     1501 cggcgacatt gttcctgtcg cgtccggaat ggcagacgtt gacgactttg atttatgcct
     1561 atttggggcg tgcgggtgag gataattatg cgcgggcaat ggtgttgaca ttgctgttgt
     1621 cggcgttcgc gctgggtatt ttcctgctgt tggacggcgg cgaaggcgga aaacagacgg
     1681 aaacgttata atgtgaaccc tttttcagag gacggcaaaa tgagcgagtt ggataacatc
     1741 cttgcccata accggcagtt tgtcgagtcg ggcgaatatg aaaaatactt taccgacaaa
     1801 taccccgaac gcgggctggc agttttgtcc tgtatggatg cgcggattat cgggctgctg
     1861 cccgacgcgt tgggtttgaa aaacggcgat gccaagctga ttaaaaatgc cggcgcgctg
     1921 gttacgcacc cgtggggttc ggtgatgcgg agtcttttgg ttgccgtgtt tgaattgaag
     1981 gtcagagaga ttatggtcat cgcccatcac gattgcggta tgcaggggct gaatgccgaa
     2041 gaattcctcg ggcgcgtccg ggaaagccag attcccgaag accgtatcga aaccctgcgt
     2101 tatgccggta tcgacctcga cggctggctg accggtttcg acaacgtcga agacagcgtg
     2161 cgccacacgg tggaccttat ccgtaaccat ccgctgatgc cgcgccatat cgccgttcac
     2221 ggactggtca tccatcccgt taccggcaaa ctgacgctgg ttgtggacgg cagtgtttca
     2281 gacggcatgg acttatcgga aggaatggaa acatcatgaa gaaaatcgga ctgttcggcg
     2341 gcactttcga cccgatacac aacgggcatc ttcatatcgc ccgtgccttt gccgacgaaa
     2401 tcggcttgga cgcggttgtt ttcctgccga caggcggccc gtatcacaaa gacgcagcct
     2461 ccgcttccgc cgccgaccgc cttgccatgg tcgaactggc gacggcagaa gacgtgcgtt
     2521 ttgccgtcag cgattgcgac atcgtccgag aaggtgcaac ctatactttt gataccgtcc
     2581 aaatcttccg ccagcagttc ccatccgcgc aactttggtg gctgatgggc agcgacagcc
     2641 tgatgaagct gcacacatgg aaaaaatggc agatgctcgt gcgcgaaacc aatatcgccg
     2701 tcgccatgag gcagggcgac agcctgcacc aaaccccgcg cgaactgcac gcgtggctgg
     2761 gcaagtccct tcaggacggc agcgtccgca tcttgtccgc cccgatgcat aatgtgtcgt
     2821 caacggaaat ccgccgcaac cttgccagcc aaggcgtttc agacggcatc ccgcctgccg
     2881 ccgcacgcta catccgcgaa cacggtttgt atgaaaaata aagtcaaatc agtaagaaac
     2941 ggctataatg ccgtctgaaa acatcccgtt aggaaaataa tgaacgaaca agaactgcaa
     3001 gacctgcaaa aaatggtcgg ggtcgccgtc aacgccctcg aagacatcaa agccaaagac
     3061 atttccgttc tcgaaacgca agacaaaact tcgctgtttg ccagaatgat tatcgccagc
     3121 ggcgacagta cgcgccaagt caaagcactg gccaacaacg ttgccgtcga tttgaaagaa
     3181 gccggttttg aaatcctcag caccgaaggc gacagcggcg aatggacgtt ggttgatgca
     3241 ggagacctcg tcgtccacgt tatgctcccc gccgtgcgcg acttctatga tatcgacacc
     3301 ctttggggcg gcgagaaacc gagtttccac gccggaatgc agaagccgtg gcacgcggca
     3361 gactgattcc cgatgccgtc tgaatgttca gacggcattt tcctgtaagg gagaaagcat
     3421 tgaacatcac cgttttggca gtcggcacca aaatgccgcg ctgggttgat gaggccgtcg
     3481 ccgaatacgc caaacgcttc ggacgcgacg tcgcctacgc actcaaagaa atcaaacccg
     3541 aaaaacgcgg cgcgggcgtg aatgccgccc aaggtatggc ggcggaagaa aaacgcatcc
     3601 ttgaagccat tccgcaaggc gcgttcctcg tcgttcttga cgaacgcggc aaagcaccga
     3661 cctccgtcga gctggcggaa cacctcaaaa gctggcagca aaacggcgaa cacgtctgct
     3721 tcgtcatcgg cggcgcggac ggtatgaccg accgcctcaa acagcaggca cgcatgatga
     3781 tgcgcctgtc cagcctgacc ctgccgcacg ggatggtgcg cgtccttctg accgagcagc
     3841 tctaccgcgc cgcctccatc ctgcacaacc atccctatca tcgggaataa gaaggctttt
     3901 gtccgtatcc ctaatcggtt aaaatcaccc cgttatttct gaacacagca aaggaatccg
     3961 ctatggcacg aatggtattc tgcgtcaagc tcaacaaaga agccgaaggc atgaaatttc
     4021 caccgcttcc caacgaattg ggcaagcgca tttttgaaaa cgtatcgcaa gaagcatgga
     4081 tggcgtggac gcgccaccaa acgatgctga ttaacgaaaa ccgcttaagc cttgccgatc
     4141 cgcgcgcgcg cgaataccta gctcagcaga tggagcagta tttcttcggc gacggcgcgg
     4201 atgccgtgca gggatacgtt ccgcaataac ggttttccgt tttgaacaca ggctgtccga
     4261 acctgcttca gacggccttt aaaatacgcc ggacaacttt atcttcacgc aaccggacaa
     4321 attttgacat tgggcaatgt taaaatcccc cattattccc aaccgcgctt tcaggagcag
     4381 atgatatgca acacgacgtt tacgactaca ccgcgcatac ggtttctaaa aacaccgtcc
     4441 tgcagaaaac ctaccgcctg ctcggatttt cattcattcc ggctgccgca ggcgcggcac
     4501 ttgccgccaa tgccggtttc aatttttacg ccgccttcgg ttcgcgctgg atagggtttg
     4561 ccgtcgtgtt ggcgtttttc tacggtatga tccacttcat cgagaaaaac cgttacagca
     4621 ataccggcgt tgccctgctg atggtattca cattcggtat gggcgtattg atcggccccg
     4681 tgctgcaata cgcgctccat attgccgacg gtgcgaaaat cgtcggcatt gccgccgcga
     4741 tgaccgccgc cgtcttttta acgatgtccg ctttggcgcg ccgaacccgg ctcgatatga
     4801 acgcgctcgg acgcttcctg accgtaggtg cggtcattct gatggtcgcc gtggttgcca
     4861 atctgttttt gggtattccc gcactcgccc tgaccatttc cgccggtttt gtcttgttca
     4921 gttccttaat gattatgtgg caggtacgca ccgtcatcga cggcggcgaa gacagccaca
     4981 tcagcgcggc actgacactg tttatctcgc tttacaacat cttcagcagc ctgctcaaca
     5041 tcctgttgtc tttaaacggc gaggattgat gccggtaaaa cgccgtccga ctatgccgtc
     5101 tgaaaatgct tcagacggca ttttactttg ggcatacaat acggatgcac gca
//
LOCUS       DAXHNE010000138         5138 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.138, whole genome shotgun sequence.
ACCESSION   DAXHNE010000138 DAXHNE010000000
VERSION     DAXHNE010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.138"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(180..425)
                     /locus_tag="ACFM4Z_001730"
     CDS             complement(180..425)
                     /locus_tag="ACFM4Z_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021728.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILRPLYIASLRRDD"
     gene            complement(426..776)
                     /locus_tag="ACFM4Z_001731"
     CDS             complement(426..776)
                     /locus_tag="ACFM4Z_001731"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021729.1"
                     /translation="MQNGGGKIYQTADNVEGIMLLKVVPERTVSADAKTRDPMWDNAA
                     LQTSEGVNFIARFLGFFSDGEYRYVDVLQPNHSDIIRYSGKDFPLNQILNHIHPARYA
                     VTFENNVDSKLRRH"
     gene            complement(783..1055)
                     /locus_tag="ACFM4Z_001732"
     CDS             complement(783..1055)
                     /locus_tag="ACFM4Z_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinosyltransferase"
                     /protein_id="HFC6021730.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPRQN
                     PFRQPDRRRYPDAVCLDYSQTILPEQRRNTGTRRVGQKIQRSRSRV"
     gene            1291..2718
                     /locus_tag="ACFM4Z_001733"
     CDS             1291..2718
                     /locus_tag="ACFM4Z_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFC6021731.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     VVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQNRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVVAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            2787..4190
                     /locus_tag="ACFM4Z_001734"
     CDS             2787..4190
                     /locus_tag="ACFM4Z_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFC6021732.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTGLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(4354..5117)
                     /locus_tag="ACFM4Z_001735"
     CDS             complement(join(4354..4862,4862..5117))
                     /locus_tag="ACFM4Z_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6021733.1"
                     /translation="MSQTAATFNLSRNTLYLWIRLKKQTGSLKHQVTGLNAVKSDRQK
                     PAQYIGQHQDAYLHEIAKHFDCTAATVCYALKQMGITRKKRPTTYKEQDPAKVTHYLT
                     QPAEFSDYQRVYLDETGFDRSLKGQIVKAQISGKRYRRLSLVSAQVGNRLIAPMVYQN
                     TMTGVFFEAWFQQCLLPALTQKSVIILDNARFHRMGVLREMAEKLGHKVLPLAPYSPE
                     LNPIEKVWANIKRYLRTVLSDYARFDDALLSYFDFN"
BASE COUNT         1291 a         1239 c         1334 g         1274 t
ORIGIN      
        1 tgttagataa ggggggaagg tttgaagcgg ttgccggctt cctgccgtcc gccggtgcgt
       61 ccgtcatcac gccggcaacc gcctttttca tcccttgctt atcttccatg gtgcgaatcc
      121 tcaaaaacgg gcaaaaaaaa gccctgttac ttgtagaaag taaaggacgt taatttttgt
      181 taatcgtccc ttcttaggga cgcaatatat aagggacgca atatataagg ttttaaaacg
      241 ctaagaacaa aatccgataa cggctctgaa gaagtaagtc tttctttatt gcacaagata
      301 gcaaacttcc acggcattct cgccccaccc gtgccgtcca agcctttttc aaagacatag
      361 atggttttct tggcaatcac ttcgtcagtt tggcggtcga taatccgtat ggtcgcacct
      421 gccactcagt gcctgcgcag cttggaatcg acattgtttt cgaacgttac cgcataacgg
      481 gcggggtgta tatggttaag tatttgattt agcggaaaat ctttacctga ataccgaata
      541 atatcggaat ggttgggttg caggacatcc acatagcggt attccccatc gctaaaaaat
      601 cctaggaaac gagcaataaa atttacgcct tcgctggtct gtaaagccgc attgtcccac
      661 atcgggtctc tggtttttgc atctgccgaa acggtacgct caggtactac cttcaacagc
      721 ataatccctt ccacattgtc cgccgtctgg taaatctttc ccccgccgtt ttgcattgtt
      781 cgttaaacac ggcttcggct tctttgtatt ttctgtccca ctcttcttgt gcctgtattt
      841 cttctttgct cgggcagaat tgtttgggaa taatccaaac aaacagcatc aggatagcgg
      901 cggcggtcag gctgcctgaa aggattttgc cggggttttt tgtaggcaaa gcggacgaga
      961 aaccaaagca acagcagcat ggtgccccaa tagccgattg agaataggat ggccaaacct
     1021 tctaggaaat ggcgtaaatc gtttgtggta aacatgggta gttcctgtgg ttaaatgtgc
     1081 aggctgcttt ttgccgaacc ttgccgcatc tcaaaagcag cctgcgcttc agcgttgcgt
     1141 tacgcagtaa aataatgaat atttgtaacg acttgggtat tttttgtcaa tattcccgcc
     1201 tttcccttaa cagctgccgc gctttccgtt aaaattcctt tacatattta tattgtttcc
     1261 tgtttctata ttgccaaggt tatacccgtt atgtttttct ccgccctgaa atcctttctt
     1321 tcccgctaca ttaccgtatg gcgcaatgtt tgggcggtgc gcgaccagtt ggaaccgccc
     1381 aaacgcacgg cggaagaaca ggcgtttttg cccgcgcatt tggaactgac cgatacgcca
     1441 gtctctgccg ctccgaaatg ggcggcgcgt tttattatgg cgtttgcgct tttggctttg
     1501 ttgtggtcct ggttcggcaa aatcgatatt gtggcggcgg cttcgggcaa aacggtgtcg
     1561 ggcgggcgca gcaaaaccat ccagccgctg gaaacggtgg tggttaaggc ggtacatgtg
     1621 cgcgacgggc agcatgtgaa acagggagaa acgctggcgg aactggaggc tgtgggaaca
     1681 gacagcgatg tggtgcagtc ggagcaggct ttgcaggctg cccaattgtc caaactgcgt
     1741 tatgaagcgg tattggcggc attggaaagc cgtaccgtgc cgcatatcga tatggcgcaa
     1801 gcacggtctt taggtctctc cgatgccgat gtgcaatcgg cgcaggtgtt ggcgcagcac
     1861 cagtatcagg catgggcggc gcaggatgcg caattgcagt cggctttgcg cggccatcag
     1921 gcggaattgc agtcggccaa ggcgcaggag cagaagctgg tttcggtggg ggcgatcgag
     1981 cagcagaaaa cagcagacta ccgccgtttg cgggccgaca attttatttc ggaacatgcg
     2041 tttttggagc agcagagcaa atcggtcagc aattggaacg atttggaaag tacgcgcggt
     2101 cagatgaggc agattcaggc ggccattgca caggcggagc agaatcgggt gctgaatacg
     2161 cagaacctga aacgcgatac gctggatgcg ctgcgccagg caaacgaaca gattgaccaa
     2221 taccgcggcc aaacggataa ggcaaagcag cggcagcagc tgatgacaat acagtcgcct
     2281 gcggacggca cggtgcagga attggccacc tatacggtgg gcggtgtggt gcaggctgcc
     2341 caaaaaatga tggtggttgc gcccgatgac gacaaaatgg acgtggaagt tttggtattg
     2401 aacaaagaca tcggttttgt ggaacaggga caggatgcgg tggtgaagat tgagagtttt
     2461 ccctatacgc gctacggtta tctgacgggc aaggtgaaaa gtgtcagcca tgatgcggta
     2521 agccacgaac agttgggctt ggtttatacg gcggtggtgt cgctggacaa acataccttg
     2581 aatattgacg gcaaagcagt gaatctgacg gcgggcatga atgtcacggc ggagattaaa
     2641 acgggtaaac ggcgggtgct ggattatctg ttaagcccgc tgcaaaccaa attggacgaa
     2701 agctttaggg agcgataggc ggatccatac tgggcatttg ttatccgccg gttcggacat
     2761 gcagactgcc tgaaaccatt gcccggatga cattgctcaa tctaatgata atgcaagatt
     2821 acggtatttc cgtttgcctg acactgacgc cctatttgca acatgaacta ttttcggcta
     2881 tgaaatccta tttttccaaa tatatcctac ccgtttcact ttttaccttg ccactatccc
     2941 tttccccatc cgtttcggct tttacgctgc ctgaagcatg gcgggcggcg cagcaacatt
     3001 cggctgattt tcaagcgtcc cattaccagc gtgatgcagt gcgcgcacgg caacaacaag
     3061 ccaaggccgc attccttccc catgtatccg ccaatgccag ctaccagcgc cagccgccat
     3121 cgatttcttc cacccgcgaa acacagggat ggagcgtgca ggtgggacaa accttatttg
     3181 acgctgccaa atttgcacaa taccgccaaa gcaggttcga tacgcaggct gcagaacagc
     3241 gtttcgatgc ggcacgcgaa gaattgctgt tgaaagttgc cgaaagttat ttcaacgttt
     3301 tactcagccg agacaccgtt gccgcccatg cggcggaaaa agaggcttat gcccagcagg
     3361 taaggcaggc gcaggcttta ttcaataaag gtgctgccac cgcgctggat attcacgaag
     3421 ccaaagccgg ttacgacaat gccctggccc aagaaatcgc cgtattggct gagaaacaaa
     3481 cctatgaaaa ccagttgaac gactacaccg gcctggacag caaacaaatc gaggccatag
     3541 ataccgccaa cctgttggca cgctatctgc ccaagctgga acgttacagt ctggatgaat
     3601 ggcagcgcat tgccttatcc aacaatcatg aataccggat gcagcagctt gccctgcaaa
     3661 gcagcggaca ggcgcttcgg gcagcacaga acagccgcta tcccaccgtt tctgcccatg
     3721 tcggctatca gaataacctc tacacttcat ctgcgcagaa taatgactac cactatcggg
     3781 gcaaagggat gagcgtcggc gtacagttga atttgccgct ttataccggc ggagaattgt
     3841 cgggcaaaat ccatgaagcc gaagcgcaat acggggctgc cgaagcacag ctgaccgcaa
     3901 ccgagcggca catcaaactc gccgtacgcc aggcttatac cgaaagcggt gcggcgcgtt
     3961 accaaatcat ggcgcaagaa cgggttttgg aaagcagccg tttgaaactg aaatcgaccg
     4021 aaaccggcca acaatacggc atccgcaacc ggctggaagt aatacgggcg cggcaggaag
     4081 tcgcccaagc agaacagaaa ctggctcaag cacggtataa attcatgctg gcttatttgc
     4141 gcttggtgaa agagagcggg ttagggttgg aaacggtatt tgcggaataa agcaggctga
     4201 aacggttatg aaattcccaa agcagcctgc accccgtttc gaaagtgcag gctgctttgg
     4261 gattgatccg atactttcac attctcattg cctttaaata aagcacagtt aaaaacacat
     4321 ttaaattaag tgcgaattaa ttcaaatgta tattcaatta aaatcaaaat aggacagtag
     4381 tgcatcgtca aatcgggcgt aatcagacaa tacggttcgc agataccgct taatattcgc
     4441 ccacactttc tcaatcgggt tgagctcagg tgaataaggt gcaagaggca ataccttatg
     4501 tcccaatttt tccgccattt cccgtaagac acccatacgg tgaaatcgtg cattatctaa
     4561 aataatcacc gatttttgag tcaatgcggg cagtaggcat tgctgaaacc acgcttcaaa
     4621 aaagactccg gtcatcgtat tttgataaac catcggagca atcagccggt tgccgacttg
     4681 tgcggacacc agagataagc gtcggtatct ttttccactt atctgcgctt tcactatttg
     4741 ccctttcagg ctgcggtcaa atcctgtttc atccaaataa acgcgttggt agtcagaaaa
     4801 ttcggccggc tgtgtcaaat aatgcgttac tttggccggg tcttgttctt tgtaagtggt
     4861 ggtctttttt tgcgcgttat ccccatctgt ttgagtgcat agcaaacggt ggctgccgta
     4921 caatcaaaat gtttggcgat ttcatgcaga taggcatcct ggtgttgccc aatatattga
     4981 gccggttttt gcctatccga tttgacggca tttagaccgg taacttgatg ttttaggctg
     5041 cctgtttgtt ttttaaggcg aatccacagg taaagcgtgt ttcttgacaa gttaaacgtt
     5101 gctgcggttt ggctgatgtt tttgcattgt tcgtaata
//
LOCUS       DAXHNE010000139         4975 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.139, whole genome shotgun sequence.
ACCESSION   DAXHNE010000139 DAXHNE010000000
VERSION     DAXHNE010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.139"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            186..1370
                     /gene="ubiM"
                     /locus_tag="ACFM4Z_001736"
     CDS             186..1370
                     /gene="ubiM"
                     /locus_tag="ACFM4Z_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HFC6021734.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNVSILTDTVVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGASSL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRASNNFPPLKKLITKQL
                     TG"
     gene            complement(1559..1714)
                     /gene="rpmG"
                     /locus_tag="ACFM4Z_001737"
     CDS             complement(1559..1714)
                     /gene="rpmG"
                     /locus_tag="ACFM4Z_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFC6021735.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(1742..1975)
                     /gene="rpmB"
                     /locus_tag="ACFM4Z_001738"
     CDS             complement(1742..1975)
                     /gene="rpmB"
                     /locus_tag="ACFM4Z_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFC6021736.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(2225..3751)
                     /gene="farB"
                     /locus_tag="ACFM4Z_001739"
     CDS             complement(2225..3751)
                     /gene="farB"
                     /locus_tag="ACFM4Z_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HFC6021737.1"
                     /translation="MDYPPLKGAALAWITLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     ALVIFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGAGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSFVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTAGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHSGGGGGH
                     "
     gene            complement(3775..4947)
                     /gene="farA"
                     /locus_tag="ACFM4Z_001740"
     CDS             complement(3775..4947)
                     /gene="farA"
                     /locus_tag="ACFM4Z_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HFC6021738.1"
                     /translation="MATHADETTIQNRQEANSAKAKRKRCLTALTLLFALAAAAAGSA
                     FFLWWQHEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVL
                     AYERAKNELIQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSA
                     EELAHARAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQ
                     RTQIRAPADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPA
                     ELVSDLYGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDV
                     DRHPLRIGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
BASE COUNT         1262 a         1414 c         1182 g         1117 t
ORIGIN      
        1 accccacacc cgatttgata cacattaagc tgaaagtaaa aatccgcata caccctcccc
       61 tgcatatttc ttcaacagtg ggttttgata taatcgccta tctgttacag atagttcaaa
      121 cggcattcaa acccttacaa ataccgtctg aaatcatcgg catccgtcta tgcaaaggat
      181 attttatgag tttacacagc gacatcctcg tcgtcggcgc aggcccggcg ggtttaagtt
      241 ttgcagccga acttgccggc agcggtttga aagttaccct gatcgaacgc agcccactga
      301 ctgttctgca aaatcctcca tatgacggac gcgaaatcgc cctgacccat ttttcccgcg
      361 aaatcatgca gcgtttgggt atgtgggaca aaattcccga aaacgaaatc taccctttgc
      421 gcgatgccaa agtgctgaac ggacgttccg actaccagct ccactttccc caaccgactg
      481 aagcgcgcgg cgaacctgcc gactgtttgg gctatctgat ttccaaccac aatatccgcc
      541 gcgccgccta tgaagtcgta tcacaactcg acaatgtcag cattctgacc gatacggttg
      601 tcaaagaagt caaaacatct gacaacgaag cacaagtcat tttggaaaac ggcaaaatac
      661 tgactgcacg cctgcttttg gcggcagaca gccgcttctc gcaaacccgt cgacaactcg
      721 gtatctcctc cgatatgcac gattacagcc gaaccatgtt cgtctgccgt atgaaacata
      781 ccctttccaa ccaacatacc gcttacgaat gcttccatta cggacgcacc atcgcactgc
      841 tgccgctgga agagcacctg accaataccg tcattaccgt cgataccgat aaaatcaaca
      901 gcgttcaaaa cctttcgccc gaagaattgg cggcaagcgt aaaagaacag ctcaagggca
      961 ggctcggcga tatggaattg gtcagcagca tccaccatta cccccttgtc ggcatgattg
     1021 ccaaacgttt ctacggcaaa cgcagcgcgc tgattggcga tgccgccgtc ggtatgcacc
     1081 ccgttacagc acacggtttc aacttggggc tttccagcgc agatattttg gcaaaactga
     1141 tactcgaagc cgaacagcgc ggtcaagaca tcggtgcatc ttcattattg gaaaaataca
     1201 gcagcaaaca tatgctgcac gcccatccgc tttatcacgg aaccaatatg atgctgaaac
     1261 tgtttaccaa tgagaccgca ccggcaaaac tgttgcgcgg cttggtattg cgtgccagca
     1321 acaatttccc accgctgaag aaactgatca ccaaacaact gaccggttga aaactgccga
     1381 aatgccgtct gaaacgtttc agacggcatt tttgtgttat gaaccgtttt gcggttttca
     1441 tatccttggc agacggtttg cagcagattc gcatacggca gatgtttcaa gcagaaagga
     1501 aacacgataa aaataaaaaa gcctccgaac agtcggaggc tttgctttca aactgaaatt
     1561 atttcagttt ggtttctttg tacactacgt gtttgcgggc aaccggatcg aattttttga
     1621 tttccagttt gccgggcata gtgcgtttgt ttttggtagt ggtgtagaag tgaccagtac
     1681 ctgcactgga ttccagtttg attttatcgc gcattgcagt aatccttaat aagggtttaa
     1741 attaagcttc accgcgagca cgcaaatcag ccaatacgac atcaatgcct actttgtcga
     1801 tggtacgcag agcggcgtta gaaacgcgca ggcgaaccca gcggttttca ctttctaccc
     1861 aaaaacgacg tgattgcaag ttaggcaaaa aacgacgttt ggttttgttg ttggcgtgtg
     1921 atacgttgtt gccagacatc gggcgcttac cggtcacttt gcaaactcgt gccatggtca
     1981 gtctccaaac ttctaaaata gtaaaacgtg atttatacca cgaaaaacag tgttagttca
     2041 agtgttttag agaaaaacgg ctgttttacg cgaattatcc tgccgattgt tgcattcggg
     2101 aaacgtttgc cgatacgaaa cagacggaat gcgattatag cgggtttcac agatgcctgc
     2161 attctaaata ctgtccgaac atatttccag tattttcaga cggcatttca agttttcaaa
     2221 tccctcaatg tccaccaccg ccgccgctgt ggaacggcgg ttttgccagc catatgacag
     2281 gaatcatgat gatgaacagc aggctgcccg ccataaagat ttcgttcgag ccgataatga
     2341 aaccctgctg ggtaatggta ttgttgataa tacctgcggt ttgaatgtcg gaaacgccgt
     2401 gctgggacaa atgggcggcc gtttcatgca atgtcgcgga atatggcgtg atgtgttcgg
     2461 caaagcgtgt atggtgcaac gcttcacgcc gttcccacag ggtactaacg aatgatacgc
     2521 cgacaccgcc cataagcacg cgcaagaagt tcgacaagct gccggcggcg gcaatctgcc
     2581 ctcccttcat gtgcgacagt gtgatggtgg tcagcggcag gaagaacatg gcaacgcctg
     2641 ccccctgcca aaactgcggc cagatgacgt tgccgatatc catgtcggca taaaaatcgg
     2701 tgcgccaata gaaagtgaag gcaaaggtca gaaagctggc ggttacgagc aggcgcatat
     2761 cgattttatt gccgaacctg ccgattaacg gagacaggaa gacagggagg atgccgacgg
     2821 gcgcggcggc aagccccgcc cacgtggagg tatagcccag gttggtctgc aacactaacg
     2881 gcagcagggt cagcgtcccc atatacacca taaaacccaa tgacgtggca attacgccga
     2941 cggtaaaatt ccgatcttta aacagcgata aatcgacaat cggatatttt tctcccaatt
     3001 cccaaacaat aaaatacgac aaacacacca gtgcgactac gcccaaggta atgatttctc
     3061 cagaggcgaa ccagtcgagt tccttacccc tgtccagcat catctgtaac gcgccgatac
     3121 cgactaccat caatgtaagc ccgacatagt cggtcggcat tttaacggtt tccgtttccc
     3181 gatatttcaa atgtttccat gtaatccatg ccgatatgat accgataggg atattaatga
     3241 agaaaatcca accccaatgc cagtttccgg aaatccaacc gccgagtatc ggcccgagaa
     3301 caggggcgac aacgacggtc attgcccaca atgccagtgc cagcgtccgt tttgcgggcg
     3361 gataggatgc cattaacagg ctttgcgaca ggggaatcag cggcccggcg ataaagccct
     3421 gcaagatgcg gaaaattacc aaagcctgaa ggttgggcgc gatgccgcac agccacgatg
     3481 tgatgacgaa accgacagcg gcggcggtaa acaatttgac ctcgccgata cgttttgcca
     3541 aaaagcccgt taacggcacg gaaacggcgt ttgccacagc aaaagaagta atgacccacg
     3601 tcccctgagt ggttgccgca ccgaggttgc cggcgataac gggaacggcg acattggcga
     3661 tagtcgtatc taaaacttcc ataaatacgg caagccccaa agacagcgta atccatgcca
     3721 atgccgcacc cttaagcggt ggataatcca ttgtgtcctc cgtttcagac ggcatcaggg
     3781 cgcggattgc ccgaggattt catcgaccgt ccgatcgact tccgaccagt cggtactttc
     3841 catttccggc aatgccgcac ccggcgtttt tgaaacaggc gcgcctgcgg cggaagtatc
     3901 cactttaacc gtcatcgaca aaccgatacg caacggatgc ctgtccacat cttcgcggtt
     3961 cagcacgata cggacgggga cgcgctgcac cactttaatc cagttgcccg ttgcgttttg
     4021 cgccggaatc agcgaaaacg cgctgcccgt acctgccgaa aaacctgcca ccctgccgcg
     4081 ataaacaatt tgtttgccgt acaaatcgga caccagctcg gcaggctgtc cgattttcat
     4141 atgccgcaac tgcgtctctt taaaattagc atccacccac acatccgaca gcggcaccac
     4201 cgccatcagc ggcgcgcctg ccgccacctg ctgcccgacc tgcaccgaac gcttcgccac
     4261 ctgaccgtcc gccggcgcgc ggatttgcgt ccgctgaagg ttcaaccacg catctttcaa
     4321 cctgccgatt gccgtctgaa ccgccggctg ttcgcgcaaa gaaacctgac cgcccaaagc
     4381 cgcacgcgcc gaagattctt ccgccaaagc cgccttaact gccgcctgag cctgagacac
     4441 tgccgcacgg gcatgtgcca gctcttcggc ggacaccgcg cccgattccg ccaaagcaga
     4501 ccggcggcgc aaatcatcct gcgcgcgtgc caaatccgcc cggcgcaagg caacctgcgc
     4561 ccccgcctgc gaagtggcgg cattttgccg gcggttttgc cgcaccgcct gaatcagctc
     4621 gttttttgcc cgctcgtaag ccagcacatc attatcgtcg tccaataccg ccagcacgtc
     4681 gccttttttc acggcatccg tatcgtcgtg caaaaccttc cgcaccgtac cgcccttttg
     4741 cggcgtaacc tgaaccacgc gtccggcaac ataagcgtct tccgtttcct cttcgtgctg
     4801 ccaccataaa aaaaacgccg acccggcggc tgcggcggca agcgcgaaca gcagcgtcaa
     4861 tgccgtcagg cagcgtttgc gtttggcttt tgcgctattg gcttcttgtc tgttttgaat
     4921 tgtcgtttca tctgcatgtg ttgccattat tccaattcca aatacttcta atact
//
LOCUS       DAXHNE010000140         4821 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.140, whole genome shotgun sequence.
ACCESSION   DAXHNE010000140 DAXHNE010000000
VERSION     DAXHNE010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.140"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(211..933)
                     /gene="rmpM"
                     /locus_tag="ACFM4Z_001741"
     CDS             complement(211..933)
                     /gene="rmpM"
                     /locus_tag="ACFM4Z_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HFC6021739.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPAPAPVVVVEQAPQYVDETISLSAKTL
                     FGFDKDSLRAEAQDNLKVLAQRLGQTNIQSVRVEGHTDFMGSDKYNQALSERRAYVVA
                     NNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPDRR
                     VDVKIRSIVTRQVVPAHNHHQH"
     gene            1297..2247
                     /locus_tag="ACFM4Z_001742"
     CDS             1297..2247
                     /locus_tag="ACFM4Z_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HFC6021740.1"
                     /translation="MKLQQLKYALEVYRHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIAQMVTSGESDLAIVTERIDDHPELGR
                     LSCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2360..3094)
                     /gene="fnr"
                     /locus_tag="ACFM4Z_001743"
     CDS             complement(2360..3094)
                     /gene="fnr"
                     /locus_tag="ACFM4Z_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFC6021741.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3295..4716
                     /gene="hemN"
                     /locus_tag="ACFM4Z_001744"
     CDS             3295..4716
                     /gene="hemN"
                     /locus_tag="ACFM4Z_001744"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFC6021742.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     FLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDDQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
BASE COUNT         1230 a         1323 c         1162 g         1106 t
ORIGIN      
        1 cgcggcaatg ggacacaaac ctgaatccga atccgaacct gcaccccgat tctgacgata
       61 gccgtttcag aaaaccaccc aaaaaaacaa accccgcaaa agcgggggtt tgtttcacat
      121 catcgatgcc gaccgacgtt tcacgaatat tgcaacagta cccggtacaa aatccgctaa
      181 cctcatgcat cggcaagata ttgcctagcc ttagtgttgg tgatgattgt gtgccggcac
      241 aacctgacgg gttacgatgc tgcggatttt cacatccacg cggcggtcag gttcgataca
      301 tgcaatcaga gcctcacgtt ttttggcttt agagactttc gcacccagtt tggcaacttc
      361 ggcttcacaa acttgagtca tttgcgcttg agattcgccc aagccgacag cagaaattct
      421 agaaacaggt acgccgttgc tgaccaggtt gtttgccact acgtatgcgc ggcgttcgga
      481 cagggcctga ttgtatttgt cagaacccat aaagtcggta tggccttcga cgcggacaga
      541 ttggatattg gtttgaccca ggcgttgcgc caatactttc aggttgtctt gagcttcggc
      601 gcgcaatgaa tccttatcga aaccgaacag ggttttggca gacagggaaa tggtttcatc
      661 aacatattgc ggagcctgct ccacaacgac gacaggcgcg ggtgcgggtt ctggctcggg
      721 ttcgggggca gcaaccgcat cgccgcattc tacgcgacct tggcttgctt tatcaaagta
      781 ggcgtttttc cagcattcgc catagttgtt gcgtacaatt tcgttcgact ggccgcttac
      841 ggtgtaaccc tgaacggacg cctcgcccgc aacagcagtg ccggaagcga gcaatgcaac
      901 gaataatgcg cttaatttca gctgtttggt cattttattc cctcattaaa tttgtacagc
      961 agatacagaa gcaactgctg aattgccaac atacaggccg ctgtagaaac atggcggcaa
     1021 atcggatatt tcagtctcca ctataaagaa gcagggggag gactgtcaat actcaacgcc
     1081 tgaaaacaac cgtaaaacag cctgtttcct gatagtctat catgccgaaa aatcaattcc
     1141 gtgtcattac tttaggggga ttttttccat atcgcacgat tgccgttgca caaaaacaac
     1201 aaacacaaca gcaaagcccc ataccgtacc ggcaagaaaa tatgataaat tataaacaat
     1261 gttcagccac ccgacacaga cccacaccga cccgccatga aattacaaca attgaaatac
     1321 gccttagaag tttaccggca caacctgaac gtttccgaag cggccgaagc cttgtttact
     1381 tcgcaacccg gcatctccaa acaaatcaaa ttgctggaag aagaaatcgg cattcagatt
     1441 tttatccgca gcggcaagcg cgtggtttcg gtctcgcagc cgggcaaggt ggttttggat
     1501 attgcagaac gtattttgcg cgatgttcag aacattaaaa atatcggcag cgagtttacc
     1561 ggacaggaca gcggttcgct gacggttgcc acgacgcata ctcaggcacg ctatgcccta
     1621 cccttgattg ttgccgattt tgtgaaacgc tatccgaaag tcaacctgac catcaaacag
     1681 gggagccctg ccgccatcgc ccaaatggtt acttcgggag agtctgattt ggcaattgtt
     1741 acggaacgta tagacgacca ccccgaactg ggaagacttt cctgctacga ctggacacac
     1801 gcggttatcg taccgaacga ccaccccttg ctcgaatgca gaaaccccct ccgtattgaa
     1861 gatttggcga ggtttccgct gattacttat gaatttgcat tcaatgcggg cagcagcatc
     1921 gcgcgggcat tttccaaagc ccgtttggaa cagccggatg tcgcattggc ggcggcggat
     1981 acggacgtat tgaagactta tgtgcgcttg ggtttgggcg tgggactgat ggcgaaaatg
     2041 gcgtacaacc cggatacgga cggcgatttg cagcttgtgg atgcggcaca cctgttcgag
     2101 ccgtcgccga cgtggattgc tttgcgcagc gatacttatt tgcgcggata tgcctacgac
     2161 tttatccaag cgtttgcgcc gcacctgaca cgcgagaagg tggataggat tctgtacacg
     2221 cccatcagcg aggatttttc gatttaggcg gctgccggtt ttcagacggc actttgcggc
     2281 agatacaaca aacaggacag atgttttcgt ctgccctgtg tttattgaga atgccgtctg
     2341 aaatgttcgt acgggttaat caaatggcgt gcgaacaacc ggataccatc ttcttcaaca
     2401 cctgcagatt gaggatcttg atgtgtttgt gttcgacgga aatcaatcct tcctgatgaa
     2461 atttagataa tgtgcggctg acggtttcaa gtttcagccc gagataactg ccgatttctt
     2521 cgcgggacat tcttaagatg aagtcgttgg cagcaaaacc tcgggaataa aggcgttggg
     2581 aaaggttcag caggaaggcg gcaatccgct cttcggcgcg catattgccc aacagcagca
     2641 taacaccttg gtcgcgcacg atttcacggc tcatcatgcg gaagaagtgc gtacgcaggc
     2701 tggggatgtt ttgccccagt tcttcgatgt gggtaaacgg cagttcgcac acttcgctgt
     2761 cttccaaggc gaccgcgtcg caactgtgca catgggaaca gatgccgtcc atgccgatga
     2821 gttcgcccga cataaagaaa cccgttacct gatcgcggcc gtcctgactg gcgacggttg
     2881 ttttgaagaa gcccgaacgg atggcaaaga gcgaagtaaa ggcttcgccg acacagaaca
     2941 ggtattcgcc ctttttcagg cggcggcttt gacggatgac ggcatcgagt tggctgagct
     3001 cgttgggcag cagcccgaca ggcaggcaga gttcccgcaa agaacaggaa gaacacagcg
     3061 ttttcatctg atgtgtagta ttatgcgaag ccataccgta cctttttgtg cgctttgccc
     3121 catcatgatt atagtggatt aaatttaaac cagtacggcg ttgcctcgcc ttgtccttat
     3181 ttaaatttaa tccactatat gtgcttattg acacatatca agacaggttt atcatactgt
     3241 ggcattctac caaactatcc aaaatttgac taacatcata aaaccacaca caccatgaaa
     3301 atcattcaga tacagaacaa tcacaatgta aacgatgacc gccccgagtt tgaccgcgcg
     3361 ctgattgcca gcctgcccgc cagcggtccg cgctacactt cctaccctac cgccgaccgt
     3421 ttccatgacg gtttccgcga aggcgaatat atcaaagctt tacatttgcg cggtatgggc
     3481 gcgttaaaca aaccgctttc cctttacatt cacattccgt tctgcaacac catctgctac
     3541 tactgcggct gcaacaaaat catcaccaaa gacaaaagcc gcgccgatgc ctacatcgaa
     3601 tttcttgaaa aagaaatgga actgctcgct ccacatctga acggacggca ccagcttgcc
     3661 caactgcact tcggcggcgg cacgccgacc tttttgagcg acgaccaaat cgaacgtgtc
     3721 ttccgcatga tacgcaaaca tttcgagtta atccccaccg gcgaatactc catcgaaatc
     3781 gacccgcgca aagtcagccg cgacaccgtc ctcatgctcg gcagactcgg cttcaaccgc
     3841 atgagcatcg gcattcagga tttcgacccc aaagtgcagg cggcggtcaa ccgcatccaa
     3901 agttacgaag aaaccaaaga agtcatcgat gcggcgcgcg aagcggggtt caaatccgtc
     3961 agcgtcgatt tgatttacgg cctgccgcac cagacttcgg aaagcatcaa aaccaccatc
     4021 gataccgttt tgtcgctcga tcccgaccgc ctcgcccttt atcactacgc ccacctgccg
     4081 cacgtgttca aaccgcaacg ccgcatcgat accgccgccg tccccgacag cgaagagaag
     4141 ctcgatatgc tgcaatactg cgtccaaacc ctaaccgaac gcggctacgt cttcatcggc
     4201 atggatcatt tcgccaaacc tgacgacgaa ctctccatcg ccctcaaaga aggcttcctc
     4261 cagcgcaact tccaaggcta ttcgacctac gcggattgcg atttggtcgc catcggcgtg
     4321 tcgtccatcg gcaaaatcgg cagcacctat tcccaaaacg aacgcgacat cgatgcctac
     4381 tatgccgcca tcgacgaagg cagactgccc atcatgcgcg gttatcagct caatcaggac
     4441 gacatcctgc gccgcaacat cattcaggat ttgatgtgcc gtttcgcgct cgactatcgg
     4501 atttacgaaa gtatgttcgg catcccgttc gaccgctact tcaaagacga actggcggat
     4561 ttggaaaaac tcgccggttt gggattggtg cgcctgaaca gccacgggct gaccgttacc
     4621 ccgaaaggac gcttcctcat ccgcaacatc gccatggtct tcgattacca cctgcgccac
     4681 aaggaaacca aagcaaaata ttcgcaaacg gtgtaattgc gtctaacgta caaatgccat
     4741 cggcaggata agtgttttca agaacaggcg gcggcatatc ataacgttcc gcacctttgt
     4801 gtccgaccgt tccgaaacca a
//
LOCUS       DAXHNE010000141         4819 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.141, whole genome shotgun sequence.
ACCESSION   DAXHNE010000141 DAXHNE010000000
VERSION     DAXHNE010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.141"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(72..554)
                     /locus_tag="ACFM4Z_001745"
     CDS             complement(72..554)
                     /locus_tag="ACFM4Z_001745"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC6021743.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(707..868)
                     /locus_tag="ACFM4Z_001746"
     CDS             complement(707..868)
                     /locus_tag="ACFM4Z_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021744.1"
                     /translation="MSGQPEKHHTSPIEDKRKNPVYRMGRAVAGFMLVVWVAVLALVF
                     FLVFRFWLS"
     gene            complement(942..1403)
                     /gene="recX"
                     /locus_tag="ACFM4Z_001747"
     CDS             complement(942..1403)
                     /gene="recX"
                     /locus_tag="ACFM4Z_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFC6021745.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            complement(1476..2186)
                     /locus_tag="ACFM4Z_001748"
     CDS             complement(1476..2186)
                     /locus_tag="ACFM4Z_001748"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFC6021746.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLSNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETA
                     TGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKDEE"
     gene            2593..3861
                     /locus_tag="ACFM4Z_001749"
     CDS             2593..3861
                     /locus_tag="ACFM4Z_001749"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HFC6021747.1"
                     /translation="MSSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRV
                     LEVNFPVKLDDGTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCC
                     VAGIPYGGGKGGITLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIM
                     SWMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGA
                     TYAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEALFKEFQEKGFITN
                     EAGYGKEITNAELLALDVDVLAPCALENQLTSENAGKVRAKIVVEGANGPTTPEADVI
                     LRQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNL
                     AQEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(3919..4725)
                     /locus_tag="ACFM4Z_001750"
     CDS             complement(3919..4725)
                     /locus_tag="ACFM4Z_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFC6021748.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
BASE COUNT         1034 a         1090 c         1428 g         1267 t
ORIGIN      
        1 cttgatttca tccgtttgca atggaaacgg atggcgcgat gtgcctgtct ttcagacggc
       61 atagtccgcc gtcagcagcc gcaggacatg tcttcagagg cttgcaggct gatgtctctg
      121 cgttttttgg ctttttcgta gcggcagcgt tggcggtcgg tcaggatttc cagcggaggg
      181 acagggacgg gtttgccgtc tttgaccgca accatcgtga agtagcagct gttggtatgg
      241 cggatttcgc cggtgcggat gttttgtgct tcgacacgga tgccgatttc catcgaggtg
      301 cgtcccgtgt agttgacagc ggcgtaaaag gtaaccaaat cgccgatgtg gatgggttct
      361 ttaaacagga ctttgtcaac cgacagggta acgcaataat tgccgctgta acggctggcg
      421 caggaatagg cgacttggtc gagcaggagc aggagttcgc cgccgtgtac gttgccgctg
      481 aaattggcgg tgtccggcat catcagttcg gacataatga gttcgtgcga aggcagttgg
      541 cgttgttgtg tcatggtgta cctgttttaa gaaagggtgt tttgaaatgg gtgcggggaa
      601 attttaggga atggggaagt ggaattcaag ctaaaaaaag ctatggcggg atagtctgca
      661 gaaatgggcc gccgatgccg tctgaagcct tcagacggca ttttgtttag gaaagccaaa
      721 aacggaagac caggaaaaac acgagtgcca atacggcgac ccaaacgacg agcatgaatc
      781 cggcaactgc ccgtcccata cggtaaaccg ggtttttgcg tttgtcttcg atgggggagg
      841 tgtggtgttt ttcgggttgt ccggacattt tgttctcaat cggaaaagtt ggaaacgggc
      901 gtaaggttat gttgccgtgc aaaaagattc aagaaggaag gtcagcagcc gtcttcccaa
      961 ttttcgtccc aagcgtgttt caatgccgtc tgaacggtat cggcatcgaa tccgcgatag
     1021 gcgaggaaac gcgcctgttt ttgtttttct ttgaggtcgg cggcggggtg tttgaatttt
     1081 ttacgcaaca cggctatggc ggcctgtttt tcgcttgagc ggtcgggaag caggttgcgg
     1141 ctggtttctt catctatgcc ctgttgcgcc aaagcctgtt tcagcctcaa tgaaccgtgt
     1201 ttgcggcttt tgctgcggat ataggcttcg gcatagcgca aatccgactg ccagttgcgt
     1261 tcggcaaatt cgtttaacac gttttccaac tcctcttcgc tttcggcgtg cggtgcaagt
     1321 ttgcgtttca gaccgatgcg gctgacttct tggcgcgaga ggatgtccat cgcgcgggcg
     1381 cgtagggatt tttgcggttt catggcggcg gtttcggcac ggaaagattc agacggcatg
     1441 ggaaacggat tccgatgccg tctgaacaga ttgttttatt cttcgtcttt atttttctgc
     1501 ggttgcaggt tttcgtaaat ttcgggcagc gagccgataa tccagtcggg gcgggtcgca
     1561 tcgtcttgcg agagcagcgt catatcgccg taaccgaagg taacgccgac gctcaggcag
     1621 ccggcggctt tggcggcgat gatgtcgttg cgcgagtcgc cgaccataac catgtttgca
     1681 acatcgatac ccaaaacttc ggcggcgtgc cgcagcggca gggggctggg ttttttctcg
     1741 ggcaggctgt cgccgccgag tatcaggctg aagtagtcgg caagtccgag ctgtttcaac
     1801 agttcgacgg caaggatttc gtttttattg gtaatgactg ccagcgggat gcccaaagat
     1861 ttgagcaatg ccagcccggt tgcggtttcg ggataggggc gggtgaagac gctcaaatgg
     1921 tcgcggtagt atttcatata ggatacgaaa cctttttccc acagttcgga atcggcttcg
     1981 cggtcgcggt cgttggagag gacgcggtga accagtttgc cgatgccgtc gcccacatag
     2041 ctttcgacca ctttggcggg cagcggtttc ataccgagtt gttccaacat cgcttctgcg
     2101 gcggcggcaa ggtcggggac ggaatcgcac agtgtgccgt ccaaatcgaa ggcgacggct
     2161 tggacgtgtt cgatggctgc attcataatc tgatttcctg tcgggttgaa agggtttatt
     2221 ttaacatcgt ttgcacaggg cttctaatgt atagattgtt aacaatcgca acaggttttg
     2281 caggcggtgt tttcggattt cggggcggaa tcgggcaagg gtttgaaatc ccagcgatgt
     2341 tccaattaaa tttttaaaaa ataaaaggat gcctatatct ttatttaatt tgacatttat
     2401 ttttcggacg cgcgcgctgc tttttcaggc ggagtccggg ctgattccgt gcggcttgaa
     2461 aaataaaatc atatatagat taatcaaatc gggcttatgc cgactacacg ctattacatt
     2521 ttgtcgggaa attggttata gtaatgagcg aaatgcacgc ccctgaaaat ctttttgtga
     2581 aaaggaagca aaatgtcgtc tgaagccctt gcaaaagaaa ccctcaatcc gtttgaaatc
     2641 gcgcgcaaac aggtcaaaac cgcctgcgac cggctgaaaa ccgatccggc cgtttatgaa
     2701 atcctgaaga gtccgacccg cgtgttggaa gtcaatttcc ccgtcaaact cgacgacggc
     2761 acggtcaaaa ccttcacggg ctaccgttcg caacacaaca acgccgtcgg cccctataaa
     2821 ggcggcgtgc gcttccatcc cggagtgaat ctggacgaag tcaaagccct gtcgatttgg
     2881 atgaccatca aatgctgcgt tgcgggtatt ccttacggcg gcggcaaagg cggtattacc
     2941 ttggatccgc gcgattattc cgaggcggaa ctggaacgca tcgctcgcgc ttattccgaa
     3001 gccatttctc cgctgattgg cgagaaaatc gatattcccg cccccgatgt gaataccaac
     3061 ggcaaaatca tgtcttggat ggtggatgct tatgaaaacg tggtgaaaca ttccgcgccg
     3121 ggcgtattta ccggaaaacc ggttgagttc ggcggctctt tggcgcgtac cgaggctacc
     3181 ggttacggcg tgaacctcgc cgccgtccaa gctttggaaa aactgggcaa ggacgtgaag
     3241 ggtgccactt acgccattca aggtttcggt aatgtgggtt accacacagg ttattacgcg
     3301 catcaatccg gcgcgaaagt tgttgcggtt tctactgtcg atgttgccat ttacaacgaa
     3361 aacggcttgg atatggaagc tttgtttaaa gagttccaag aaaaaggctt catcaccaac
     3421 gaagccggtt acggcaaaga aattaccaac gccgaacttt tggctttgga tgtggacgta
     3481 ctcgcccctt gcgcgctgga aaaccaactg acttccgaaa acgccggtaa agtccgcgcg
     3541 aaaatcgtgg tcgaaggcgc gaacggcccg actacgcccg aagccgacgt tatcctgcgt
     3601 caaaacggcg tattggtcgt gcctgatatt ttggcgaact gcggcggcgt ggtggtatct
     3661 tattttgaat gggtacaaaa cctgcaaggc tattattggg agtttgacga agttcaagag
     3721 aaagaaaccg tcgtcctgcg acgcgcgttc cgcgatatat ggaacttggc tcaagaatac
     3781 gatgtcgatt tgcgaaccgc gtcttacatg atgagtatcc gccgtgttga aaaagcgatg
     3841 aagctgcgcg gttggtattg atgtttgaac aaaaaatgcc gtctgaagcc ttcagacggc
     3901 atttttgtgc gggcgggatt aatggtgcga atggccgatt tggaaggtta aagtcgagta
     3961 gttcgcctgt ttttggcaca cgctttgatc ggggaagtcg gctttgtgtt cgacattggc
     4021 tttccagaag ccttggcgca aggggatgat gtccacttcg cctttgtcgt ctgtgctgtc
     4081 ggagaaagcc tgtgcttcgg ttttgtgcgt tttgctgcgg tcgctggtgt cgaagccgtc
     4141 aaaggtggcg gtaacggtgg cattgggcag cggttcgcca cggaacagaa cgcggacttt
     4201 gaagcgttcg cctacgtgaa tgttggcggg attgtccagc gggacgattt ccaagttttg
     4261 tccgaccggt ttggtgatga tggcggtgtc cgcgctttcg tgtccgacgt tgacgatgtt
     4321 tttgccgaac attcgggttt gttcgcaata gcttgcgtca ggcatttctt tgatgcccgc
     4381 ctgtttccag cctgctttgt tttttgacca gaaagtaggc tgatattcgg cgatgacgag
     4441 gtaactgccg tccttaacgg gacggttgct tcggtactgg tagttgtatg tgccgcgttg
     4501 aatcatgttt tccttgcctt tttcggtaac cagctgcatc ggtttgctga agatgtgcag
     4561 gcggtctttg gcgatgggtt cgagttcggg aaattcgccg tagcccaagt cggctttaag
     4621 gtattcgccg ccgtgcgtgt gggcggtttc gacccagacg cggtgggcgt gggcggatgt
     4681 ggcaaatagg gcggaaacgg cgagcagtgt caatgttttc ttcatggtgc agcctttgtc
     4741 tcgtgtgggt taaagatttt gttatgttat aacaaaatag gtatcgtgtg aagatggatt
     4801 tgctgtctga aaactgtct
//
LOCUS       DAXHNE010000142         4574 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.142, whole genome shotgun sequence.
ACCESSION   DAXHNE010000142 DAXHNE010000000
VERSION     DAXHNE010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.142"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(58..1851)
                     /locus_tag="ACFM4Z_001751"
     CDS             complement(58..1851)
                     /locus_tag="ACFM4Z_001751"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFC6021749.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAEAQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGAALQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKLKAQGSGTI"
     gene            2096..3205
                     /gene="proB"
                     /locus_tag="ACFM4Z_001752"
     CDS             2096..3205
                     /gene="proB"
                     /locus_tag="ACFM4Z_001752"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFC6021750.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITCQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIATESGVPVYICSSLKPDALAEAADNQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     GVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            3218..4480
                     /locus_tag="ACFM4Z_001753"
     CDS             3218..4480
                     /locus_tag="ACFM4Z_001753"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFC6021751.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAAAADI
                     LAANRQDLEAAAGNIPESMTDRLLLDEKRIGAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGRESSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIEAHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT         1045 a         1257 c         1271 g         1001 t
ORIGIN      
        1 gccgcaatgc cgtctgaagc ctttttatgc ttcagacggc atcgtctgtt ttcacgctca
       61 aatcgtaccg ctgccttgcg ctttcagttt ggcggcacta aattccagtt tgctcaaggc
      121 ggaaaggtag gctttggcgg tagcgaccaa aacgtcggta tccgcgccct gaccgttgac
      181 aacgcggttg ccgcgcgcca gacggacgct ggtttcgccc tggctttccg taccttgcgt
      241 gacggcgttg acggaataaa tctgcaaagc cgcgccgctt tgcgccacgc tttcaatcgc
      301 tttgaaaatc gcatccacgg ggccggagcc tgtggcggaa gcgcgttttt cttcgccttt
      361 gatgctgaac acgatgtcgg cgcgcggctc ttcgccggtt tcggtgctga ttttttggga
      421 gatgaatttg tagctctcgg cattcatgct gcccatttcg tcggatacca gtgcgtgcag
      481 gtcttcatcg aagatttcgc gttttttgtc ggcgagttct ttgaagcggg caaacgcggc
      541 gttcagtgcc tcttcgcttt ccaactcgat gcccaaatcc gccagcttgg ttttgaaggc
      601 gttgcggccg gacaatttgc ccaagctcaa acggtttgtt gcccagccga ccgattcggc
      661 ggacataatc tcgtaagttt cgcggtgttt cagcacccca tcctgatgga tgcccgattc
      721 atgcgaaaag gcattggcac cgacaatggc tttattgggc tgcacgggat agcccgtaat
      781 agtggacacc agtttggacg aaggcacgat ttgcgtggta tcgatgccgg tttccaagcc
      841 gaacaagtcg tggcgcactt tcaacgccat cacgatttct tcaaccgaag cattgcctgc
      901 acgttcgccc aagccgttga cagtacattc cacctgacgc gcgccgcctt ttaatgcggc
      961 aagcgaattg gcaaccgcca agcccaaatc gttgtggcag tgtgccgacc aaacgacttt
     1021 gccgccgttg ggcgttttgg caatcagttc gcggaaaaat tcttcggttt tatacgggat
     1081 ggaatagccg acggtatcgg gaatattgat ggtggtcgcg cccgcttcaa tcaccgcgcc
     1141 gcagatttcg gcaaggaaat cgatttccga acgcaacgcg tcttcgcagg aaaattccac
     1201 atcgtcggtg tattcacgag cgattttcac cgctttgacc gccgcctcaa tcacctgctt
     1261 cggcttcatc ttcaatttgt actccatatg gatggggctg gtggcgatga aggtgtggat
     1321 gcgttttttc ggcgcgggcg caacggcctc acccgcctga cggatgtccc gctcgatggc
     1381 gcgggacaat gaacagaccg ttgatttggt aatggttttc gcaatcgcat tgaccgcctc
     1441 gaaatcgccc gggctggcag cggcaaaacc cgcttcgatg atgtccacac ccaatttttc
     1501 cagctggcgg gcgacgcgga ttttttcctc tttggtcata gcggcgccgg gcgactgctc
     1561 gccgtcgcgc agggtggtgt cgaaaatgat aacgcggttg gcctgtgtca tttctgttct
     1621 ctccagagat tcgttttttt gtaaaaaagc attcaaatcc agcggcctgc cttgcgcttc
     1681 cgccaacgcg gtcagctttt gcagttgcgc cagaggcagc gagccgcgcg tgcgccattt
     1741 atagatagcg gcggtcgtcg cggcattttc gggatcgtgc tgtttcaacg cttcggcaag
     1801 cgcgttcacg ccgccgaaat aagcaaccaa gcggtctata tctaattgca tggtagtcct
     1861 tgtctaaaat aaatcagcct acagcagaaa tatggacagg attatacgca ttttggacaa
     1921 aacggcaata taaatttgaa aaatctcaat aagccgccat agattttatt aattttatac
     1981 attttgtcca ttttattgca gacgcggcgt gattgttgac aattgcggca gaacagggca
     2041 aattcggcgg cacaaggtgg caacgagaca ggacaaacca acaaggacgg aaacaatgaa
     2101 atacaaaaga atcgtattta aagtcggcac atcttcgatt acccattcgg acggcagtct
     2161 ctcgcgcggc aaaatccaaa ccatcacctg ccagcttgcc gcattgcatc atgcgggaca
     2221 cgagctggtc ttggtgtctt ccggcgcggt tgcggcaggg ttcggtgcgc tgggtttcaa
     2281 aaaacgtccg gtcaaaatcg ccgacaaaca ggcttccgcc gccgtcgggc aggggctgct
     2341 gatggaagaa tatacggcaa acctgtcttc agacggcatc gtgtccgcgc aaatcctgct
     2401 cagccgcgcc gactttgccg acaaacgccg ctaccaaaat gccggcggcg cactttccgt
     2461 gctgctgcaa cgccgcgccg tccccatcat caatgaaaac gatacggttt cggttgagga
     2521 attgaaaatc ggcgacaacg acacattgag tgcgcaagtg gcggcgatga tacaggcaga
     2581 cctcttggtg ctgctgaccg acatagacgg tctttacacc ggcaacccga acagcaatcc
     2641 cgatgccgta cggctggaca aaatcgaaca catcaaccat gaaatcatcg aaatggcggg
     2701 cggctcgggt tcggcaaacg gcacaggcgg tatgctgact aaaatcaaag cggcgacgat
     2761 tgcgaccgag tccggcgtac cggtctatat ctgttcctcg ctcaaacccg atgcacttgc
     2821 cgaagcggca gataatcagg cggacggctc gtttttcgtc ccccgtgcca aaggtttgcg
     2881 gacgcagaag caatggctgg cgttctattc cgaaagcagg ggcggcgttt atgtggacga
     2941 aggtgcggaa cacgctttgt ccgaacaggg aaaaagcctg ctgatgtcgg gcattgccgg
     3001 aatcgaaggg catttttccc gtatggacac cgtaaccgtg tacagcaagg caaccaaaca
     3061 gcccctaggc aaagggcgcg tcctgttcgg ttctgccgcc gccgaagacc tgctcaaatc
     3121 gcgtaaggcg aaaggcgtgt tcatccatcg ggacgactgg atttccatca cgcccgaaat
     3181 acgcctgctt ctgaccgaat tttagaaaag gaaacccatg tcaaacacac aaaaacagct
     3241 tgcccttgcc aaagcggcaa aaaaatccgt caacacggcg gatacggaag aaaaaaaccg
     3301 cgcgctgctt gctatggcgg acagcttgga agcggcggcg gcagatattt tggcggcaaa
     3361 ccgtcaagac ttggaagccg cggcaggcaa cattcccgaa agcatgaccg accgcctttt
     3421 gttggacgaa aaacgcattg gcgcgatggc ggacggcatc agggcggttg ccgcgctgcc
     3481 cgaccccgtg ggcgaaatac tggaaacctc gactttgccc aacggcttgg aaatcgtcaa
     3541 aaagcgcgtg gcgatgggcg ttatcggcat tatttacgaa agccgcccga acgttacttc
     3601 cgatgcggcg gctttggcac tgaaaagcgg cagcgcggtc gtactccgca gcggcaaaga
     3661 tgcattccaa tccgcacgcg ccatcgttgc cgccctgaaa acggggttgg cacaaacccg
     3721 catcgacccc gacgcgctcc agttgattga agacaccggg cgcgaaagca gttacgaaat
     3781 gatgagggcg aaagattatc tagatctgct gattccgcgc ggcggggcgg ggctgatacg
     3841 ggcggtggtt gagaatgccg tcgtgccggt cattgaaacg ggaacgggca ttgtccacat
     3901 ttatatcgac aaggacgctg attgggataa ggcactccgt atcgtgtaca acgccaaaac
     3961 cagccgtccg tccgtgtgca actcgatgga agtgctgctg gtgcatgaag acattgctgc
     4021 cgacttcctg cccaagctcg aacgactgtt ggttcgcgac cgcatagaag ccggactgcc
     4081 gcccgtccgc ttccgtttgg atccgcaggc ggcgcggcat atcggcggcg aagcggcggg
     4141 tgcagacgat ttcgataccg agtttttaga ctacatcctc gccgtgaaaa ccgtcgcatc
     4201 ggtcgaagag gcggtcgggc acatcgaagc ccacggcaca caccattcag acggcatcgt
     4261 taccgaaaac cgccacgctg cggactattt cacgacccat atcgattctg ccgccgtgta
     4321 tgtcaacgcg tccacgcgct ttaccgacgg cggcgaattc ggcttgggtt gcgaaatggg
     4381 catctccacg caaaaactcc acgcccgggg cccgatgggt ttgaaagagc tgacgagtta
     4441 caaatacatc gtacaaggca cgggacaggt cagggaataa tccgaattca aatgtctgat
     4501 atagtggatt aactttaaac cggtacggcg ttgcctcgcc ttagctcaaa gagaacgatt
     4561 ctctaaggtg ctga
//
LOCUS       DAXHNE010000143         4570 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.143, whole genome shotgun sequence.
ACCESSION   DAXHNE010000143 DAXHNE010000000
VERSION     DAXHNE010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.143"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(52..273)
                     /locus_tag="ACFM4Z_001754"
                     /pseudo
     CDS             complement(52..273)
                     /locus_tag="ACFM4Z_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine catabolism dioxygenase TauD"
     gene            complement(272..379)
                     /locus_tag="ACFM4Z_001755"
                     /pseudo
     CDS             complement(272..379)
                     /locus_tag="ACFM4Z_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(425..1153)
                     /gene="pdxJ"
                     /locus_tag="ACFM4Z_001756"
     CDS             complement(425..1153)
                     /gene="pdxJ"
                     /locus_tag="ACFM4Z_001756"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFC6021752.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(1177..1968)
                     /gene="recO"
                     /locus_tag="ACFM4Z_001757"
     CDS             complement(1177..1968)
                     /gene="recO"
                     /locus_tag="ACFM4Z_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFC6021753.1"
                     /translation="MSEYRVNHEPVFMLASSPWRESSLWVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSVSWYGSQELKTLHRAEWVGGWRQPQGRALFGGLYVNELVLK
                     LTAREDPVPELYDALAEVMEAVCCKAAYIDDLRRFEWRLLNLLGVAPDLNRDGDGGTI
                     AAGGTYLVRPETAVFPVGKGFAVPPHAAGVVAPGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(2005..3132)
                     /gene="pheA"
                     /locus_tag="ACFM4Z_001758"
     CDS             complement(2005..3132)
                     /gene="pheA"
                     /locus_tag="ACFM4Z_001758"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFC6021754.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDDCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEIVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(3171..4403)
                     /locus_tag="ACFM4Z_001759"
     CDS             complement(3171..4403)
                     /locus_tag="ACFM4Z_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFC6021755.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLIVYCLAVAAIVFVSSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRVTAWRL
                     KTGVHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENGQSEYL"
BASE COUNT         1021 a         1304 c         1261 g          984 t
ORIGIN      
        1 cgccttgtcc tgatttttgt taatccacta tattacataa tcatcgcctc aatatttaat
       61 atcccaaaat atagatttaa aaccatcgtc tttgtggttt gtcagcattc ctaattgaga
      121 tagaaagtca aataatttct tgtttctccg gctgtcatca agatggttta gattaagacc
      181 gcgaacaata caatacccaa tactctgtac tgtattgtga atttcttcta tcaaactatt
      241 attgtttgca gataaaatat acttttgatt catatagtga attaaattta aaccagtacg
      301 gcgttggctt gccttgccgt actatttgta ctgtctgcgg cttcgccgcc ttgtcctgat
      361 ttaaatttaa tccactataa catatccctg tcgtgaaatg ctgtctgaaa gggtttgcct
      421 gcccttacgg cagcagcctt gccctgaaca tcgcctcctt catttggcgc acggcttcgg
      481 gcagtccgag gaagagggct tgggcaatca gcgaatgccc gatgttcagt tcgcggatgg
      541 cgaggatttg ggcgatgggg gtaacgttgt gtatggtcag tccgtgtccg gcgttgacga
      601 ccaagcccaa atcgccggca aaatgcgcgc cgttttgcag acgctcgaat tgcttgagtt
      661 gttcggcgtg gctgcgcgcg tcggcatacg cgcctgtgtg cagctcgaca acgggcgcgc
      721 cgacatcata ggcggcttgg atttgcctgt cgtcggcatc gataaagaga gagactcgga
      781 tgcctgcgtc ggtcaggatt ttggtgaacc cggcgatttt ttcctgttgc gccaatacgt
      841 ccaagccgcc ttcggtcgtg atttcctgac gtttttcagg cacgatgcac acgtcttccg
      901 gcatcacttt aagcgcgttt tcgagcattt cttccgtcaa cgccatttca aggttcaggc
      961 gcgtgcggat ggcgtttttg acggcaaaca cgtccgcgtc tttgatgtgg cggcggtctt
     1021 cgcgcaggtg catggtaatc aaatccgcac cgtgcgtttc ggcaaccagt gccgcctcca
     1081 cggggctggg ataagtcgta ccgcgcgcat tgcggacggt ggcgatgtgg tcgatgttga
     1141 cacctaaaag cataatcttt ccttttattt ctgccttcag acggcatttg aagccgtgcc
     1201 gtccgaagtc gggacggttt cccgggcggt ttctttgcgg tcaaactgcc gtatctgttc
     1261 caacacctgc cgcgatttca gcccctcggg caacaggtgg cggataaaaa gccgtgtgat
     1321 tttcaatgcc tgttgcaggc tttcggcagt gcggaaactg ccttcgcgca aatcgatcag
     1381 gctctgcccg ggggcgacaa cgccggcggc gtgcggcggt acggcaaatc cttttccgac
     1441 ggggaagacg gctgtttccg ggcggacaag gtatgtgccg cctgccgcaa tcgtcccgcc
     1501 gtccccgtcg cggttcaaat cgggggcaac gcccaacagg ttcagcagcc gccactcgaa
     1561 acggcgcaag tcgtcgatat aagcggcttt gcagcacacc gcctccatca cttccgccaa
     1621 cgcgtcgtat aactcgggca ccgggtcttc gcgggcggtc agtttcaaca ccaactcgtt
     1681 cacatacaat ccgccgaaca acgccctgcc ctgaggctgc cgccaaccgc cgacccattc
     1741 ggcgcggtgt agggttttga gttcctgact gccgtaccac gacacgctga cgggcacgaa
     1801 cggcaccaat acgccgcgca gctcgctctg ccttttgcgc gcgctgcgcg ccagcaaagc
     1861 cacacgcccg taacggcggc tgaatgcttc aacccacagg ctgctttcgc gccagggcga
     1921 agatgccagc ataaaaacgg gttcatggtt gactcggtat tcggacataa tcgttgggga
     1981 aatgccgtct gaacgctgcc gccgctacaa aacggcggtc gggtacgaac cgatgacttt
     2041 gacgaacgaa gcgcgttcgc ccaagcgttc caatgccgtc tgaatctgcg cgtcccggcg
     2101 gtgtccttcg atgtcgatga agaacaggta ttcccacaaa acggatttgc tcggacggct
     2161 ctcaaacttg gtcatggaaa tacccgattc ggtcagcggt tgcagcagcg aggcaaccgc
     2221 gcctgcccgg ttgggcgcgg aaacggccag cgaagtcttg tcgctgccgc ttgcaccggt
     2281 ttcgtgatgt cccatcacca agaagcgcgt ggtgttgttc ggttcgtctt cgatgcactc
     2341 ggcaaccata tcgagtccgt agatttccgc cgccgtgcgt ccggcgatgg cggcaaccgt
     2401 accgtcgtcc gattcggcaa ccagccttgc ggcttcggca ttgctggaca cggcaatccg
     2461 ttcggcgttg ggcaggtgtc tgcccaacca gtcgttgcac tgcgccaacg cctgcgcgtg
     2521 ggaaaagact ttggcaatgc cttcggtgct gccgttgttt ttacgcaaaa ggttgtggtg
     2581 gatgcgcaaa acgatttcgc cgcacgcctg caacgcggta acggcaagca ggtctaacgt
     2641 gcgaccgacc gagccttcgg tcgaattttc cacgggggcg accagataat ccgcctgacg
     2701 cgtttcaacc tgcttgaagc agtcgtctat ggtcggacac gccatggtgt gcgcggcgtg
     2761 tccgaaatgt ttgattgccg cctgctgggt aaacgtgccc tgcggcccca gataggcgat
     2821 ggtcagcggg cgttcgacgg cgaggcactc gctcatcact tcccgaaaca ggcgtgctac
     2881 cgattcgtcg ggcagcgggc ctttgttcaa atcctgaatg cggcgcaaca cggcgacttc
     2941 gcgttcgggg cggtacactg cgcccgtgcc tttcagctcg ccgatggcgt gggcgtgttg
     3001 cgcacgttcg ttgagcaggc gcaggatttc ggcatcgatg gtgtcgatgg cgttgcggtg
     3061 gggaaggagg agttcgtcga tagtttggga cataatttcg cctgaacagt aatttatcgt
     3121 gcattctaac atcaaatgcc gtctgaaacg gtttcagacg gcattttccg ttaaaggtat
     3181 tcgctttgcc cgttttcttt ccacgcacga tgcgagcaga gccacagaag cgcaatgccg
     3241 caagaggtgg acgcggtcat cgttgccgcc atcacggttg ccgaaccgtc gtgcaagaag
     3301 gtcgccgcca tacccacccc cgcgccgatt aaagattgga atacacccaa tacggcgttt
     3361 gcgctgccgc cctcttcttt gaaataggac ataaaacacg cctgcgtgtt tgcaccgacc
     3421 aagccctgcg taccgacgga aaacatcacg cacgcgacca gcagccaaaa cgggggcaac
     3481 ccgaaaaaca gcacggcggc gagttgggac aggttggcgg caaactggac gacaatcccc
     3541 cacagcagga tgctttgcgg atgcacgccg gttttgagcc gccacgcggt aacgcggttg
     3601 aaaaacatca tcgtgatgat gttgagtgca aacgcccaag cgtattgatg aggcgtaaca
     3661 cggtagagct gctggtacac gaaggaagat tcggtcagaa aggcgaacat cgaaccgaag
     3721 ctgaatgcct gaaaaaacag ataacccatc gcagcacggg ttttcaatac gcgcttgaac
     3781 cgccccgcca ccagcccgaa cacgtcccgt ccgattttgc cgccgacggc gggcttgggc
     3841 aggaaatact gtaccaaacc gagcagcacc agcgaatacg ccgccagaaa aacaaaaatc
     3901 gcctgccagc cacccaagcc ctgcaacaat gcgccgacca tgggtgcgac cagcggcaca
     3961 accatcaaaa tgatgccgat aagggcaaac atctgggcgg ctttgcgtcc ggaataataa
     4021 tcgcgcacca ttgcgccgac gatgaccaca gtcatgcccg caccgaatgc ctgcacgacg
     4081 cgcaggttga ggagctgttc ggcactcgaa acaaatacga tggcggcaac ggcaaggcaa
     4141 tatacaatca aaccggtcag ggcgacgggt ttgcgccctt tgatgtcgga caccgaaccg
     4201 ccgaccacct gtccgaacgc cgtgccgaac ataaacaaac tcaaactctg ttcgatgcgg
     4261 tgaacatccg cgttcagcga ttgcgccatt tcgggaatcg cgggcaggta ggcatcgatg
     4321 gaaaacggca tcagcgtaac cagcatcgcc atcaaaaccg ccatcagttt ttcgctcatt
     4381 tcaggataat gggcagaagg cataaattct cgtccgattc gcaaacaaac cgcccggcgg
     4441 cggaaaacac aatacgcgga aacggaaaat tttaacacac cggcacaaac aggcggcaac
     4501 aaaagccccg gcaaaaccgg acgaaccgga acgatatagt ggattaacaa aaaccagtac
     4561 agcgttgcct
//
LOCUS       DAXHNE010000144         4569 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.144, whole genome shotgun sequence.
ACCESSION   DAXHNE010000144 DAXHNE010000000
VERSION     DAXHNE010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.144"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(180..1118)
                     /locus_tag="ACFM4Z_001760"
     CDS             complement(180..1118)
                     /locus_tag="ACFM4Z_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6021756.1"
                     /translation="MLELNGLCKRFGDKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NIIAGIVRPDGGEIWLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRGTLRRMTAERIRNGGIPAVLVTHSPEEACTTADEIAVMHKGRILQYGTPET
                     LVKTPSCVQVARLMGLPNTDDNRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMRHAGAVSGKDTVRIHIEEREIVRFR"
     gene            complement(1127..2002)
                     /locus_tag="ACFM4Z_001761"
     CDS             complement(1127..2002)
                     /locus_tag="ACFM4Z_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4743 domain-containing protein"
                     /protein_id="HFC6021757.1"
                     /translation="MPTVRFTESVSKHDLDALFEWAKASYGAESCWKTLYLNGLPLGN
                     LSPEWAERVQKDWEAGCSESSDGIFLNADGWSDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGKRSPHKAVDPDK
                     LDNIAAGGVSGGEMPSEAVCRESSEEAGLDKTLFPFIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAALPETFLPENQDGEVACFEKMDIGSLVAAMLSENMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(2184..2260)
                     /locus_tag="ACFM4Z_001762"
     tRNA            complement(2184..2260)
                     /locus_tag="ACFM4Z_001762"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2224..2226),aa:Met,seq:cat)
     regulatory      2410..2509
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2601..4502
                     /gene="thiC"
                     /locus_tag="ACFM4Z_001763"
     CDS             2601..4502
                     /gene="thiC"
                     /locus_tag="ACFM4Z_001763"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFC6021758.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRG
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGVYGDPAAHINLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGTERAHDPKTAHLRFNQITRPRRAKSGSNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKSEYAKLLKQHAGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1104 a         1307 c         1199 g          959 t
ORIGIN      
        1 tcgggaaaaa cggtttcttt agattttacg ttctagattc ccgcctgcgc gggaatgacg
       61 atgaaaagat tgttgttgtt tcggataaaa ttttgcagcc ctgataaaaa aatatggctg
      121 ctttggtaaa aaaatgccgt ctgaaaggtt ttcagacggc attttgtttt taagaagcat
      181 cagcggaagc ggacgatttc ccgttcttcg atatggatgc gtaccgtatc cttgcccgat
      241 accgccccgg cgtgccgcat atcgaggttc agccacagga tgccgtgttc cggatggagg
      301 acggacaggc tgaacgattc gggcaaacag gtacgggata atacgcggca ctccatgccg
      361 tcttggtcga aacgcaccgc atgttgcgga atatggcggt tatcgtcggt attgggcaaa
      421 cccatcagtc gggcgacctg cacgcaggat ggtgttttga ccaatgtttc gggcgtaccg
      481 tattgtagaa tcctcccttt atgcatcacg gcgatttcgt ctgccgtcgt acaggcttct
      541 tcgggcgaat gcgttaccaa aacggcaggg atgccgccgt ttcggatacg ttcggcagtc
      601 atacggcgca gcgtgccgcg caaatgcgtg tccaaactgg aaaacgattc gtccaacagc
      661 agcagggaag ggcggacaac caaagcgcgc gccaacgcca gccgttgctt ctcgcctccg
      721 gaaagttttt caggcttgcg gtgcgcctcg ttttccagtc cgacttcggc aagtgccgcc
      781 atggcgaggc gttcggcttc ggctttcggc attttttgca ttttcaaacc gaatgccgca
      841 ttttccagcg cactcatatg gggaaacagc gcgtaatctt gaaacatcag cgagatacgg
      901 cgtttttcgg gcggcatacg ggtaatgttt tctccgttca gccatatttc cccgccgtcc
      961 ggccggacaa tccccgcaat tatattcagc agggtggatt ttccgcagcc cgaccgcccc
     1021 aaaacggcga gtattttgcc gcgcccgaca gtcaggcaga tgttgtcggc gacggtttta
     1081 tcgccgaagc gtttgcagag tccgttcagt tcaagcatag cgcatcctat aaacgtatgc
     1141 cgtccagcca ctcggacagc ggatgggcgg catcaatcag accgtaacgg caaaacgcgt
     1201 ccagcgtaac cagttgcgcg tcgtgcatca tgttttccga caacatggcg gcaaccaggc
     1261 tgccgatgtc cattttctca aaacacgcaa cctcgccatc ctgattttca ggcaggaagg
     1321 tttcgggcag agcggcatcg aatacataca ggatttcatt gtggacgccg cggctgacgg
     1381 ggcgcaggct gtgcagccgg gataccggac ggataaacgg aaacagcgtt ttatccaaac
     1441 cggcttcttc gctgctttcg cggcacacgg cttcagacgg catttcgccg ccggaaacac
     1501 cgccggcggc aatattgtcg agtttgtcgg gatcgactgc tttgtgcgga ctgcgcttgc
     1561 ctatccagaa atgccatcgg ccgtccgatt cggtcagtcc gttgagatgg acggcgcggc
     1621 tgagcagtcc gaacggacgg aaagcggcgc gttcgagcgt gaacaagggg ttgccgccgc
     1681 cgtcggtcag gtcgaaacac tcgttgcgcc atccgtgaag cagccccgcc ttgttccatg
     1741 tgcgggcgag gtgctgcaag cggccgccca tatctgacca gccgtccgca ttcagaaaaa
     1801 tgccgtctga agactccgag cagcctgcct cccagtcttt ctgaacgcgc tccgcccatt
     1861 ccggcgacag gtttcccaaa ggcaggccgt tcagatacag cgttttccag caactttccg
     1921 caccgtaact tgcctttgcc cactcgaata gggcatcaag gtcgtgtttg ctgacggatt
     1981 cggtaaaacg gacggtcggc atagtcggct ttcttgaaca taatgcggaa gattgtaatc
     2041 aaaccgcctg aatttgcaac tgcaaacaga cggcgggctg ccgttccgat attgcggcag
     2101 gaaggaaaat ccggcaaaaa acggaggcgg caggaaacag gcaaaacaaa acccctcggc
     2161 attgaaccga ggggtttgat atttggttgc gggagcagga ttcgaaccta cgaccttcgg
     2221 gttatgagcc cgacgagcta ccatgctgct ccatcccgcg tcagaagatg agactatacg
     2281 gcagattttt tatgttgtca aacattactt ccgcgaaaaa tcataaaatt ttgccgtccg
     2341 gcggtttatg cctgatttga catattattt tctttacaaa agttcatgtt cgtgatttaa
     2401 ttttggttaa catcgaaaca ggggtgctgc ctgatgttca ggcggctgag aaataccctt
     2461 tacacccgat cgggataata cctgcgtggg gagttttcac ggattctgtc tttcagacgg
     2521 cattggtttt caaatgccgt ctgaaaacgc aaaacgctcc tgtttcttta ttctaaacga
     2581 gaaaacagga gcattttttt atgactacgc caaaaaaaac cgccaaaact tccggcaacg
     2641 aagcgcgcga gcttgccgac ttgagcgaag acatcggcat ccgcttcaaa tatccgaact
     2701 cggaacgcgt gtatctgcaa ggcagccgtg gcgacatccg cgtgcctttg cgcgaaatcc
     2761 gtcaggacga cacctatacg gcgcaaggta cggaagccaa tccgccgatt cccgtctatg
     2821 acaccagcgg cgtgtacggc gatccggcgg cacacatcaa tttaaaacaa ggtctgcccc
     2881 acatccgcac ggcgtggctg gacgaacgcg gcgataccga aatcctgccc aaactctcca
     2941 gcgaatacgg caccgaacgc gcacacgatc cgaaaaccgc ccatctgcgt ttcaaccaaa
     3001 tcacccgccc gcgccgcgcg aaaagcggca gcaacgtaac ccagcttcac tacgcgcgcc
     3061 aaggcattat cacgcccgaa atggagtttg tcgccatacg cgaacgttta aaattagacg
     3121 aattgtccca aaaatcggaa tacgccaaac tcttgaaaca acacgcaggg caaagtttcg
     3181 gcgcgaacat cccaacccgt cccgaccaaa tcacgcccga attcgtgcgc caagaaatcg
     3241 ccgccggacg cgcgattatc cccgccaaca tcaaccaccc cgaactcgaa ccgatgatta
     3301 tcggccgcaa cttccgtgtc aaaatcaacg gcaacttggg caattccgcc gtcacttcca
     3361 gcctgaccga agaagtcgaa aaaatggtgt ggtcgctgcg ttggggcgcg gacacaatta
     3421 tggacttatc cacaggcgcg cacatccatg aaacgcgcga atggattatc cgcaacgcgc
     3481 ccgtccccat cggcaccgtg cccatctatc aggctttgga aaaaaccggc ggcattgccg
     3541 aagatttgac ttgggatttg ttccgcgaca ccttaatcga gcaggcggag caaggcgtgg
     3601 actatttcac catacacgcg ggcgtgttgc tgcgttatgt gccgatgacc gccaaccgcc
     3661 tcaccggcat cgtctcgcgc ggcggttcga ttatggcgaa atggtgtttg gcacatcatc
     3721 gggaaaattt cctctacacg catttcgacg aaatctgcga aattatgaaa gcgtatgacg
     3781 tatcgttcag cctcggcgac ggcctgcgcc ccggctgcat tgccgatgcc aacgacgaat
     3841 cccaattcgc cgaactgcac accttgggcg aattgaccga taaagcgtgg aaacatgacg
     3901 tacaagtcat gatcgaaggc cccggccatg tgccgctgca acgcgtcaaa gaaaacatga
     3961 ccgaagagct gcaacactgc tttgaagcac ctttttacac gctcggcccg ctcgttaccg
     4021 acatcgcacc cggctacgac cacatcacct cgggcatagg cgcggccaat atcggctggt
     4081 acggcacggc gatgctttgt tacgttaccc ccaaagaaca tttggggctg cccgacaaag
     4141 aagacgtgcg taccggcatc atcacctaca aactcgccgc ccacgccgcc gatctcgcca
     4201 aaggctggcc gggcgcacaa ttacgtgaca acgccctgag caaagcgcgt ttcgagttcc
     4261 gctggcgcga ccaattccgc ctcagcctcg accctgaacg tgccgagagc ttccacgacg
     4321 aaaccctgcc tgccgaaggc gcgaaaatcg cccacttctg ctcaatgtgc ggccccaaat
     4381 tctgctcgat gaaaatcacg caggaagtgc gcgactacgc cgacaagcaa aaagcccaac
     4441 ggcagggcat ggaggaaaaa gcggtcgagt tcgtcaaaaa aggcgcgaag atttacagtt
     4501 gaaacgtcaa gcaaaaaatg ccgtctgaaa cccggaaaaa aggcttcaga cggtattctt
     4561 tcgcttgtg
//
LOCUS       DAXHNE010000145         4512 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.145, whole genome shotgun sequence.
ACCESSION   DAXHNE010000145 DAXHNE010000000
VERSION     DAXHNE010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.145"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(728..1543)
                     /locus_tag="ACFM4Z_001764"
     CDS             complement(728..1543)
                     /locus_tag="ACFM4Z_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6021759.1"
                     /translation="MNPAPKNLLFSSLLFSSLLFSSLLFSSLLFSSAAQAASEDSSRS
                     PYYVQADLAYAAERITHDYPQATGANNTSTVSDYFRNIRAHSIHPRVSVGYDFGGWRI
                     AADYASYRKWKESNFSTKKVTKEINDNYQETKTEHQGNGSFHASSSLGLSAIYDFKLN
                     DKFKPYIGARVAYGHVKHQVHSVETKTTTVTSKPKNGSPQGGAVPSRESSKPPYHESN
                     SISSLGFGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYHF"
     gene            1873..2605
                     /locus_tag="ACFM4Z_001765"
                     /pseudo
     CDS             1873..2605
                     /locus_tag="ACFM4Z_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
     gene            2721..4229
                     /gene="ppx"
                     /locus_tag="ACFM4Z_001766"
     CDS             2721..4229
                     /gene="ppx"
                     /locus_tag="ACFM4Z_001766"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFC6021760.1"
                     /translation="MTTHPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEAV"
BASE COUNT         1079 a         1168 c         1233 g         1032 t
ORIGIN      
        1 ctggattccc gcctgcgcgg gaatgacggg tttcaagatt gcagtgttgt cgggaatgac
       61 gggtttcaag attgcggtgt tgtcgggaat gacgaatcca tccatacgga aacctgcacc
      121 acgtcattcc cacggaagtg ggaatctaga atcccggggt ttcagtcatt tccgatagat
      181 tcccgccgcg tcgggggtct agattcccgc ctgcgcggga atgacgggtt tcgagattgc
      241 ggtgttgtcg gaacgcaact gaaccgtcat tcccacgaca gtgggaatct agaatctcgg
      301 gggttcagtc atttccgata gattcccgcc gcgtcagggg gtctagattc ccgcctgcgc
      361 gggaatgatg ggtttcaaga ttgcggtatt gtcgggaatg acgaatccat ccatacggaa
      421 acctgcacca cgtcattccc acgaaagtgg gaatctagaa tcccggggtt tcagtcattt
      481 ccgatagatt cccgccgcgt cagggagtct ggattcccgc ctgcgcggga atgacgaatt
      541 tcgagattgc ggtattatcg ggaatgacga atttcgagat tgcggtattg tcgggaatgg
      601 cgggtttcaa gattacggtg ttgtcgggaa tgacggttcg ggtatttcca cgcccgcccc
      661 gcgcctgtaa acggcaggtg aatcaaaaat gccgtctgaa ggttcagacg gcatcggtgt
      721 cggggaatca gaagtggtag cgcatgccca atgagacttc gtgggttttg aagcgggtgt
      781 tttccaagcg tccccagttg tggtaacggt atccggtgtc caaggtcagc ttgggcgtga
      841 tgtcgaaacc gacaccggcg atgacaccaa aacccaagct gctgatgctg ttgctttcgt
      901 gatagggagg tttgctggac tctcttgatg ggacagctcc tccctgtgga gagccgttct
      961 ttggtttaga ggtaacagtc gtggtttttg tttccaccga atgaacttga tgtttaacgt
     1021 gtccgtaggc gacgcgcgca ccgatatagg gtttgaattt atcgttgagt ttgaaatcgt
     1081 aaatggcgga taagccgaga gaagaagagg cgtggaagct gccgtttcct tgatgttctg
     1141 ttttggtttc ttggtagttg tcgtttatct ctttagtaac ttttttagta gaaaaattac
     1201 tttctttcca ttttctgtaa ctggcataat ctgccgctat cctccagccg ccgaaatcgt
     1261 agccgaccga cacccggggg tggatggaat gcgcacggat gtttctgaaa tagtcgctta
     1321 ctgtgcttgt gttgtttgca ccggttgctt gcggataatc gtgggtaatg cgttcggcgg
     1381 cataggctaa atccgcctgc acataatacg ggctgcggct gctgtcttca cttgccgcct
     1441 gcgctgcgga agagaagaga agagaagaga agagaagaga agagaagaga agagaagaga
     1501 agagaagaga agagaagaga aggttttttg gggctggatt cattttcgac tccgtattcg
     1561 gttttaactg attaaaaaga acgattttca atgatgttgc aggagcggac tatatcaggt
     1621 ttggggcgat gtttcaacac aatatagcgg atgaacaaaa aagagaacga tgctctaagg
     1681 tgctgaagca ccgagtgaat cggttccgta ctatctgtac tgtctgaggc ttcgtcgcct
     1741 tgtcctgatt tttgttaatc cgctataaac aacgcttcgt ccaaaaaaac gattgaattt
     1801 gcgggcagaa gctggacgaa aaccgccgac agcctgccgc aaaaggcaca cggtttgcgc
     1861 tagggcttag gcgtgtcgcg cgaaatcaat gcgggcgggc atcatttcct ctacggcggc
     1921 atcagcggcg gcggcgtgca ttattgggat aacaaagatt tcagcgaaca gtgcctgcac
     1981 ctgtcgctcg gctataaaaa tcgttcggta acgcgctcgt tcggcatcgt gccgtttgtc
     2041 gggcaaaacc tcttaggcgg cagccgatac aatttcgtcg gcggctttaa tgccgatttc
     2101 tcccaacact tgagcgaacg ctggcggttg acactaaacg cgggcaatat gtggaagcat
     2161 tatcaggaag accgcaccgc cgcccggtac gacagccata tgccgctggc gggcgcgacg
     2221 ctgatgtatt ccgcgccgaa agactggctg ctttacggcg gggcggactg gtcgcacgac
     2281 ttgacgaagg aggcggaaca ggcttccgtc cgcaagggtt tgcgtgtcgg cgcagtcaaa
     2341 acgttcgacg gcgggcttgg gtctgcgggc aaacctgcgc tatacccgca ggatgtttga
     2401 cgcacccggg accattgtgt accgcttccc gcgcaaagac cacgaatatc aggcaaacct
     2461 gtcgttgtgg cacgacaaaa tctcttggaa gggctttacg ccgcaactca atttccgcta
     2521 tctgaaaatc gacagcaata tgaaaagttt ttacacacgc aaaaacacgc agattttcat
     2581 gagcgtggaa aaggatttca aataagcgca aaaatgccgt cggcaacatc cgtgggcaga
     2641 atcaaaaacc gccgcatcat ttattgtcaa cgcctgcgcc gtcagagtaa cattgcgttt
     2701 ttcccccacc ggtatccacc atgaccaccc atcccgcaaa cgtcctcgcc tccgtcgatt
     2761 tgggttccaa cagtttccgc ctccagattt gcgaaaacaa caacggacaa ttaaaagtta
     2821 tcgattcgtt caaacagatg gtgcgcttcg ccgccggtct ggacgaacag aaaaacctga
     2881 gtgccgcttc ccaagaacag gctttggact gcctggcaaa attcggcgaa cgcctgcgcg
     2941 gcttccgccc tgaacaggtg cgcgccgtgg caaccaatac attccgcgtc gccaaaaaca
     3001 tcgcagattt ccttcccaag gccgaagcgg cactgggttt ccccatcgaa atcatcgcag
     3061 ggcgcgaaga ggcgcggctg atttataccg gcgtgatcca caccctcccc ccgggcggcg
     3121 gcaaaatgct ggttatcgac atcgggggcg gttcgacaga atttgtcatc ggctcgacgc
     3181 tgaatcccga cattaccgaa agcctgccct tgggctgcgt aacctacagc ctgcgcttct
     3241 tccaaaacaa aatcaccgcc aaagacttcc aatctgccat ttccgccgcc cgcaacgaaa
     3301 tccagcgcat cagcaaaaat atgaggcgcg aaggttggga tttcgccgtc ggcacatcgg
     3361 gttcggcaaa atccatccgc gacgtgcttg ccgccgaaat gccccaagag gcggacatta
     3421 cctacaaagg catgcgcgcc ctcgccgaac gcatcatcga agccggttcg gtcaaaaagg
     3481 ccaaatttga aaacctgaaa ccggaacgca tcgaagtttt tgccggcgga cttgccgtga
     3541 tgatggcggc gtttgaagaa atgaaactcg acaggatgac cgtaaccgaa gccgccctgc
     3601 gcgacggcgt gttttacgat ttgatcgggc gcggtttaaa cgaagatatg cgcggacaaa
     3661 cggttgccga gttccaacac cgctaccacg tcagcctcaa tcaggcgaaa cgcaccgccg
     3721 agaccgcgca aacctttatg gacagcctct gccacgctaa aaatgttacg gttcaagagc
     3781 ttgccttgtg gcaacagtat ctcggacgcg ccgccgcgct gcacgaaatc ggtttggaca
     3841 tcgcccacac cggctatcac aagcattccg cctacatcct cgaaaacgcc gatatgccgg
     3901 gtttctcacg caaagaacag accatacttg cccaactggt catcggtcat cgcggcgata
     3961 tgaaaaaaat gagcggcatc atcggcacca acgaaatgtt gtggtatgcc gttttgtcgc
     4021 tgcgccttgc cgcactgttc tgccgttcgc gccaagacct gtctttcccg aaaaatatgc
     4081 agttgcgtac ggacacggaa agctgcggct tcatcctgcg tattgacagg gaatggctgg
     4141 aacgccatcc cctgattgcc gacgcattgg aatatgaaag cgtccaatgg caaaaaatca
     4201 atatgccgtt caaagtcgag gccgtctgaa ccttgcgggg caaatgccgt ctgaatgatg
     4261 ttcagacggc atttccctgt ccgcaacatc ccgatatgcg cggcacatct gctcggaacg
     4321 gtcatgcagg cgtaaaaaac aaggggcata caactcaaaa accgcctgaa aatcttcagg
     4381 cggtttcgtt tgggttgccg gcaggcggca tcccatcatt tttgccaagg caacaaatta
     4441 tttggcggca tctttcattt tgtctgccgc ttcctgagtc gcgtcggcag ctttgttcaa
     4501 agtatcttta gc
//
LOCUS       DAXHNE010000146         4445 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.146, whole genome shotgun sequence.
ACCESSION   DAXHNE010000146 DAXHNE010000000
VERSION     DAXHNE010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.146"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(63..1205)
                     /locus_tag="ACFM4Z_001767"
     CDS             complement(63..1205)
                     /locus_tag="ACFM4Z_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFC6021761.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELGSLGCTDVQAFDGGVSCRGGL
                     EQAYAANLHSRTAGRILLHLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLNERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLLAGYDGTQPFQDPFCGSGTIAVEAAWIATGRA
                     PGMMRRFGFEKLQNFDKTLWTDLRRRAEAQIRPVRAPIAGSDSDRRIVQTALDNARSA
                     EVDDIVSFSVADAQSVRPNGENGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WRVGMFTSDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVEGSNR"
     gene            complement(1254..1676)
                     /locus_tag="ACFM4Z_001768"
     CDS             complement(1254..1676)
                     /locus_tag="ACFM4Z_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021762.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPVGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1669..2478)
                     /locus_tag="ACFM4Z_001769"
     CDS             complement(1669..2478)
                     /locus_tag="ACFM4Z_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC6021763.1"
                     /translation="MIQDTRPLIRVVAGILLNRDGDYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHAHVCLKFLWVNPDQWTGEPQ
                     SREGQAWSWQKAGDFTIAPMLPANGALLRSLSVPRRLYGSLKTGLYGENSMGAYRVLP
                     LGSAEESGANVLMEAAQWQDRPEHADSVWMMVQTREQWRQAQEKGADAVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2560..2937)
                     /gene="acpS"
                     /locus_tag="ACFM4Z_001770"
     CDS             complement(2560..2937)
                     /gene="acpS"
                     /locus_tag="ACFM4Z_001770"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFC6021764.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(3137..3904)
                     /locus_tag="ACFM4Z_001771"
     CDS             complement(3137..3904)
                     /locus_tag="ACFM4Z_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6021765.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFIMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3914..>4445)
                     /locus_tag="ACFM4Z_001772"
     CDS             complement(3914..>4445)
                     /locus_tag="ACFM4Z_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HFC6021766.1"
                     /translation="DITFSEDVGECPLHSDSSFSENPESYLVMYVVKSANDGGNSLFL
                     SSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPTSFDKKQGVIWGNILSLNTQMIRFRS
                     DCIYKGIEENRNKVSKEMVLALDYLVNVIKNASDIQEFSAQDDGLIIIDNVNGLHART
                     DYTDKNRHYIRARITV"
BASE COUNT         1087 a         1101 c         1091 g         1166 t
ORIGIN      
        1 tcggccggat attaaaaaaa gccgtctgaa catttttcag acggcttttt tgtacacttt
       61 cctcaacggt tcgatccttc caccatatca atcaggaaca ggcggcaatc gaggttgccg
      121 ttataaagcg ggattttgcg ttttggcgac aggcgcatga atttgggcat ttccctgtcg
      181 ctggtaaaca tgccgacccg ccagccggca tagtgttttt tcaaccacgt ccccagctgc
      241 ggataaagag cctgcaaagc gcggacttcc tcaaggcgca caccgtaggg cggattggac
      301 accatgatgc cgttttcgcc gttcggacgg acggattgcg cgtcggcaac gctgaaggaa
      361 acgatgtcgt ccacttcggc actgcgtgcg ttgtccaatg ccgtctgaac gatgcggcgg
      421 tcgctgtcgc tgcctgcaat cggggcgcgg acggggcgga tttgcgcttc ggcgcggcgg
      481 cgcaaatctg tccacagcgt tttatcgaaa ttttgcagtt tctcaaaacc gaaacggcgc
      541 atcatacccg gcgcgcggcc tgtggcaatc caagcggcct cgacggcaat cgtgccgctg
      601 ccgcaaaacg ggtcttgaaa cggctgcgtg ccgtcgtagc ctgccaagag cagcagtccg
      661 gcagcaaggt tttcgcgcag cggggcttcg ccggtatcca ggcggtagcc gcgtttaaac
      721 agggcttcgc ccgaagtgtc gataaagatt tcgacattgc gttcgttcaa aaaggcgtgg
      781 atgcggacat cgggcgcggc tttgtccacg ctcggacgtg cgtcgtaaat gtcgcggaaa
      841 gcgtcgcaga cggcatcttt gacggtcagt ccgacaaatt ggatgctctt aacgttggca
      901 cgctttgcct cgactttgac tttgaacgtc tgctgcaagg taaaccagtt gaaccagttg
      961 atgtttttgg caagtttgta aatgtcgcgc tcggttcggt atgtgccttt ggtcaggtgc
     1021 agcaggatac ggccggcggt gcgcgagtgc aggttggcgg cgtaggcctg ttccaatccg
     1081 ccgcggcagg aaacgccgcc gtcaaacgct tgaacatcgg tacagccgag gcttccgagt
     1141 tcttgagata aaacggtttc caagccgcgc gggcaggtgg cgaaaagtgt atataaagta
     1201 ttcataacgt gtcctttccg gacggatgtt ccggcaatgg cggattgaaa ggtttattcg
     1261 gcggtaaatg ccgtctgaaa tgtccggctg ccgattgtaa tgtctgccgt aaaatcgcgc
     1321 ctgccttcga cacagatggg caggcggatg cgtactgcct gccaagtccc cgacggttgc
     1381 cgctcgaaca tatagcggtt gaaacccata tccatatttt tcatactgaa gctgatgctg
     1441 acctgttccg tgccgacggg cgcgtgttcg atataaatat caaacggttt tttggttgaa
     1501 acggcggcgg cgcggacgcg gcttccgtcc ggcagcgtgc aaccctcggt caaatcgcat
     1561 tgcgccgcca cggcttgcgg ctgctgcgcc tgccaccatt gcaacaaaac gagcttgacg
     1621 gcggcaaagg caatcagcag cagtgcggca agcagtaatt tacggttttt attcatgatt
     1681 cttccctatt gtttcatccc ttaccaccac gtgcgccccc aatccgagcc atagttttcc
     1741 ataacgtgca accgtctgtc cgtttgctgc aagtacgagc ggcacggata cgccctgccg
     1801 cagggcttct gccgcctctt gtgcctgaac atcatcgcac acgcgccaaa cgaccgcatc
     1861 cgcgcccttt tcctgcgcct gccgccattg ttcgcgggtc tgcaccatca tccacacgct
     1921 gtcggcgtgt tcgggtctgt cctgccattg cgccgcctcc atcaaaacgt tcgcaccgct
     1981 ttcctctgcc gaacccaaag gcaggacgcg gtacgcgccc atactgtttt ctccgtacaa
     2041 acctgttttc aggctgccgt acaaacggcg cggaacggac aacgaacgca aaagcgcgcc
     2101 gttggcgggc agcatggggg caatggtaaa atcacccgcc ttctgccacg accatgcctg
     2161 tccttcgcgc gattgcggtt cgcccgtcca ttggtcgggg tttacccata ggaatttcag
     2221 gcagacatgc gcgtgttcgt aggaatggat tttggtcagc caaggcgtgg cggcgaggat
     2281 gcggatgccg agttcttctt caaactcgcg ttgcaaggct tggaagtcgg tttcgcccgc
     2341 ttcgaccttg ccgccggcaa attcccaata tccggcatag ggtttacctt cggggcggga
     2401 actgagcagg tagtcgccgt ctcggttgag caggatgccg gcaacgacgc ggataagggg
     2461 tcgggtgtct tgaatcatgg cgggtacgcg gttggtcggt cggagtgcaa atgagggacg
     2521 ggcaatgccg tcaacgggtt tgccgcattt cagacggcat tatttttcgg caacgacaaa
     2581 cgccaatacg gtgtcttctt cgtcgctcat gctgaggctg acgcggctga tgccttgttc
     2641 ctccagccat ttggacaggg cggggccgta gaaaaattcg ggcttgccca atgcgtcatg
     2701 cccgatgccg atgttgcgga aggaaaccgc gccgcgtatg cccgtgccga cggctttggc
     2761 aaaggcttct ttggcggcaa agcgtttggc aaggtagttg acgggtttgc ccgcctgagg
     2821 aaattcaagc agctcttccg gagtgaggat gcgcccggca aacgcctgtc cgaatttttt
     2881 gtttaagcgg atgatgcgct tgagggaaac aatgtctgtg ccgatgccgt aaatcatatt
     2941 tgcgctcctt cgcccttggt tgcgggtagt gatgaatgtg atgatgaatg ccgtcaattt
     3001 cttggggcgt taaaatggcg tgaatatcca ctttcggggt tttgtcggta acaattttta
     3061 ttgtaacctt ttgcatttga aattctaaaa catcgtgcca attatttaat aaggagttaa
     3121 ggcatttaat aatgaattaa agtttgatta aacccgaatg cccgattttc agagccaata
     3181 ccaataaaac aataaaaatt aacattttaa aaaaatttgg gctaatctta gtccttaacc
     3241 gaattccaac atacaatcca ataacagaca atacggacag taaaaatatt aaaccgtatt
     3301 cactcttatt taataaccaa tactggtctc ttagcatata tatttgaaca attttcgcca
     3361 aaagatagca tagattgctt gattttacga tacgattttt attttctgtt tcgctaagca
     3421 aaaatattaa caatatggga gacatggcat tggttgaacc gccgatgatg cctgccaaaa
     3481 acccaaaaag aaccatattc ttattattgg caactacttg aatatttttt gcttttgcac
     3541 atacatttaa aataccattg acagaataat acaatgtaat gattgccatc agtaaaagca
     3601 gccaagacac tggaagtatc aaaagcaact tcacccccaa aatgctgcca acgacgctgc
     3661 cgatagcaag caatttatag gtttttaaat aataaacaat ctcttgccaa aaaccctttt
     3721 tgttattgct gcatagaacc aacaagctca ttaacaggct tggtaatgcc accaaggcaa
     3781 caaccttaga caatggcatg ataaaagcca atgcggttgt accgagcatc ggaaatccca
     3841 tgcctgtaat tccgtgcagt attgcggcag caacaaaaac gatagattgc attatttctt
     3901 gcataaccgt cctttataca gtaattcttg ctctaatata atgcctgttt ttatccgtat
     3961 aatcagttct ggcatgcaag ccattgacat tgtcaataat aatcaaacca tcatcttgtg
     4021 cagaaaattc ttgaatatca ctcgcatttt ttataacatt tacaagataa tcaagtgcaa
     4081 gtaccatttc ctttgatact ttatttctat tttcttcaat acctttatag atacaatcac
     4141 ttctaaatct aatcatttgt gtattgagcg ataagatatt accccatatc acaccttgtt
     4201 ttttatcaaa tgatgttggt gttttaaatg gatataaatt gcccgttaat gtttttaagt
     4261 gttttttacc ggtttctgtt ttagataact gattgacaat atctgatgaa cttaaaaata
     4321 gggaattacc tccatcattg gctgatttta ctacatacat aaccaaataa ctttccgggt
     4381 tttcactaaa agatgaatca ctatgaagtg gacattctcc aacatcttcc gagaaagtta
     4441 tatca
//
LOCUS       DAXHNE010000147         4421 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.147, whole genome shotgun sequence.
ACCESSION   DAXHNE010000147 DAXHNE010000000
VERSION     DAXHNE010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.147"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            40..463
                     /locus_tag="ACFM4Z_001773"
                     /pseudo
     CDS             40..463
                     /locus_tag="ACFM4Z_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            569..1132
                     /gene="pgsA"
                     /locus_tag="ACFM4Z_001774"
     CDS             569..1132
                     /gene="pgsA"
                     /locus_tag="ACFM4Z_001774"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFC6021767.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLAAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NILMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1212..1287
                     /locus_tag="ACFM4Z_001775"
     tRNA            1212..1287
                     /locus_tag="ACFM4Z_001775"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1245..1247,aa:Gly,seq:gcc)
     gene            1313..1388
                     /locus_tag="ACFM4Z_001776"
     tRNA            1313..1388
                     /locus_tag="ACFM4Z_001776"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1346..1348,aa:Gly,seq:gcc)
     gene            1412..1487
                     /locus_tag="ACFM4Z_001777"
     tRNA            1412..1487
                     /locus_tag="ACFM4Z_001777"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1445..1447,aa:Gly,seq:gcc)
     gene            1509..1584
                     /locus_tag="ACFM4Z_001778"
     tRNA            1509..1584
                     /locus_tag="ACFM4Z_001778"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1542..1544,aa:Gly,seq:gcc)
     gene            1610..1683
                     /locus_tag="ACFM4Z_001779"
     tRNA            1610..1683
                     /locus_tag="ACFM4Z_001779"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1642..1644,aa:Cys,seq:gca)
     gene            1786..1875
                     /locus_tag="ACFM4Z_001780"
     tRNA            1786..1875
                     /locus_tag="ACFM4Z_001780"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1820..1822,aa:Leu,seq:taa)
     gene            1979..3031
                     /gene="sohB"
                     /locus_tag="ACFM4Z_001781"
     CDS             1979..3031
                     /gene="sohB"
                     /locus_tag="ACFM4Z_001781"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFC6021768.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(3082..3948)
                     /locus_tag="ACFM4Z_001782"
     CDS             complement(3082..3948)
                     /locus_tag="ACFM4Z_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HFC6021769.1"
                     /translation="MWKMLKHIAQTHRKRLIGTFSLVGLENLLMLVYPVFGGRAINAV
                     IAGEVWQALLYALVVLLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSRKFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLKNSLERDNHFIRKGDRRQLYRHYGLLARLRVLISNREAFGYLCVG
                     TAMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQR
                     IGGLERNIKAGT"
     gene            complement(3959..4390)
                     /locus_tag="ACFM4Z_001783"
     CDS             complement(3959..4390)
                     /locus_tag="ACFM4Z_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFC6021770.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEKWSLPKQTVSGVCKTLAGQGLIEWQEGEQDRRKRLLSLTETGKAYAAPLTE
                     SAQEFSDKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
BASE COUNT         1092 a         1047 c         1159 g         1123 t
ORIGIN      
        1 tatcgctggg tatggtttgc cgccataagc tgtctgctgt tgtccgccgt ttttattgca
       61 ccttacctga cggcatttca cgaacaagaa aaaacattag aatatgcgga attgaccgtt
      121 accgccccca atcgcagcgg acgggcaatc aaactggatg cagacgggca gcattaccgg
      181 ctttcctgct atgggttcga cagtttgtgc acaggcggca atatcggcag aaccatcagg
      241 gcggagcagg ttagagccgt tttaagcgaa accgtcggca aaggtttttt aaacggcgtt
      301 ctgttggaat accgcaacag tggcagtgtc tataagcaat aaagattttg cccgcacgga
      361 agaccgcctt gtcgaagtgt tggcacaacc tgccgttttc agcctgaaac tgggtatttt
      421 gcttttattg cccgccattt tcctgcgttt gaaaagaatg tgaaaacaaa ttggcggggc
      481 gggggaatgc ccatgcggca attttacgtt ttgggttatg aggataaccg ttataatcac
      541 aactttacag tctgcacgga gaaaaccgat gccttggaat ctccccatct tcctcacatg
      601 gttgagggtc ttgctcattc ctgtcatgac agcccttttt tacctgcctt ttccgtggtt
      661 cgcggaggaa acgattaatc tcgccgccgc cgttattttt gccgttgccg ccttaaccga
      721 ctggtttgac ggatttttgg caagactgtg gaaacagact tccgatttcg gcgccttcct
      781 cgatcctgtc gccgacaagc tgatggttgc cgtatcgcta cttttactgg tcaaactcga
      841 ccggacctat gttttgtttg ccatgatcat catcggcagg gaaattacca tttccgcatt
      901 gcgcgaatgg atggcgcaaa tgggcaaaag gaacagcgtt gccgtcgcca ccgtcggtaa
      961 gtttaaaacc gccgcgcaaa tgctggcaat tttcctgctg ctgctgaata ttcccgattt
     1021 ttacggattt aatcttgttg ttatcggcaa catattgatg tttatcgcat ctttgctgac
     1081 ggtttggtcg atgctgtatt atctgaaaat ggcgtggaaa gaaatcgcct gaaaaaaaca
     1141 taaaaatagc ttgacggcaa aaacagaatc cataataatt gcgtctcttc gatgttggag
     1201 aatgaaatcg ggcgggaata gctcagttgg tagagcgcaa ccttgccaag gttgaggtcg
     1261 cgagttcgag actcgtttcc cgctccaaaa atttcatttt cccacaatca ttgcgggaat
     1321 agctcagttg gtagagcgca accttgccaa ggttgaggtc gcgagttcga gactcgtttc
     1381 ccgctccaag ttttatttca aacatatcaa cgcgggaata gctcagttgg tagagcgcaa
     1441 ccttgccaag gttgaggtcg cgagttcgag actcgtttcc cgctccaaaa accgatatgt
     1501 ttgaacgcgc gggaatagct cagttggtag agcgcaacct tgccaaggtt gaggtcgcga
     1561 gttcgagact cgtttcccgc tccaagtttt ttagataagc cagttttagg gcgagatagc
     1621 aaagtggtta tgcagcggat tgcaaatccg tctacgccgg ttcgattccg actctcgcct
     1681 ccattatcgt actatggcgg ggtggcagag tgtttatgct atgaggagtg caatcttcat
     1741 acaggccggt taaaatccgc gcccccgcct ccacctttca caaatgcccg ggtggtgaaa
     1801 taggtagaca caacggactt aaaatccgtc gggactaaac atcccgtgcc ggttcgattc
     1861 cggctccggg caccaagctg aaaatgacaa tgccgctcca agcggttatt tttttatctg
     1921 tcggacggtg atggacaata ttgacatgtt catgcctgaa caagaggaaa tccaatcaat
     1981 gtggaaagaa attttactga attacggtat tttcctgctc gaactgctta ccgtgttcgg
     2041 cgcaattgcg ctgattgtgt tggctatcgt acagagtaag aaacagtcgg aaagcggcag
     2101 tgtcgtactg acggattttt cggaaaatta taaaaaacag cggcaatcgt ttgaagcatt
     2161 ctttttaagc ggggaagagg caaaacatca ggaaaaagag gaaaagaaaa aggaaaaggc
     2221 ggaagccaaa gcagagaaaa agcgtttgaa ggagggtggg gagaaatctg ccgaaacgca
     2281 aaaatcccgc ctttttgtgt tggattttga cggcgatttg tatgcacacg ccgtagaatc
     2341 cttgcgtcat gagattacgg cggtgctttt gattgccaag cctgaagatg aggttctgct
     2401 tagattggaa agtccgggcg gcgtggttca cggttacggt ttggcggctt cacagcttag
     2461 gcgtttgcgc gaacgcaata ttccgctgac cgtcgccgtc gataaggtgg cagcgagcgg
     2521 tggttatatg atggcgtgtg tggcggataa aattgtttcc gctccgtttg cgattgtcgg
     2581 ttcggtgggt gtggtggcgg aagtaccgaa tatccaccgc ctgttgaaaa aacatgatat
     2641 tgatgtggat gtgatgacgg cgggcgaatt taagcgcacg gttactttta tgggtgaaaa
     2701 tacggaaaag ggcaaacaga aattccgaca ggaactggag gaaacgcatc agttgttcaa
     2761 gcagtttgtc agcgagaacc gccctcaatt ggatattgag gaagtggcaa cgggcgagca
     2821 ttggttcggt cggcaggcgt tggcgttgaa cttgattgac gagatttcga ccagtgatga
     2881 tttgttgttg aaagcgtttg aaaacaaaca ggttatcgaa gtgaaatatc aggagaagca
     2941 aagcctgatc cagcgcattg gtttgcaggc ggaagcttct gttgaaaagt tgtttgccaa
     3001 acttgtcaac cggcgggcgg atgtgatgta gtttgccgaa cggatggaaa tgccgtctga
     3061 agcgtgttca gacggcattt ttcaagttcc ggctttgatg ttccgttcca atccccctat
     3121 ccgttgtccg atgtctttca aattggaata ttgttcgacc aatcgcggca cgtcgtccaa
     3181 actcatggca aacatccaca gataagtgcc gaccgaatag acatgccccg cgctgctgta
     3241 gcctttgagc gtcatcatca caaaagcaaa gccgaacaaa atacccatcg ccgtgccgac
     3301 gcagagatag ccgaaggctt cgcggttgga aatcagcaca cgcaggcgcg caagcagtcc
     3361 gtaatggcgg tacagctgcc gccggtcgcc ttttcggata aagtggttgt cgcgttccaa
     3421 gctgttcttc aggcggaaat acaggttttc gctgatggcg gcaaaacgtg gcaaaagcca
     3481 taaaaacaac gcaagtatgc ccaccgccga cacgccgacc caaaattcca gcaccagcag
     3541 catgatgcac gcaccgaata tggatacgac ggatgtcgcg gcaatcggca ggtgttcttc
     3601 aaaaaagctg acaaacttac gtgacagggc aacccgtgca gttaccgccg aatgcgggac
     3661 ttgtcgctgc cgctgttcca acacaaccgg cacggcgatt tcggtataaa tccgcgtaaa
     3721 cgtgcgcgta tcggcaatcc gccgcgccgc accgaccagc cacattaaaa gcacaaccaa
     3781 agcgtacagc aacgcctgcc acacctcccc cgcaatcacg gcattgatcg cccggccgcc
     3841 aaacaccgga tacaccagca tcaaaaggtt ttccagtccg accagggaaa atgtgccaat
     3901 caatcgcttg cggtgggttt gggctatgtg tttcaacatt ttccacatat ttgcccccct
     3961 attttttatt ttccgagatt gttttttcca tcacttcagc cagtgcatcc aaatcggcaa
     4021 acagccgagt tgtgcgcttg tcgccgaatg tggcaaatac tttgtcgctg aattcctgcg
     4081 cgctttctgt taaaggtgcg gcataggctt tgcctgtttc ggtcaacgac agcaaccgtt
     4141 tgcgccggtc ctgttcgcct tcctgccatt caatcaaccc ttgtccggca agggttttgc
     4201 atacgcctga aacggtctgt ttgggcaggc tccacttttc gccgatatgc ttttgcgtgc
     4261 ggctgccttc ggttgccagg gtataaagta cggcaaagag gttgtaattc agatcctgct
     4321 gcccgatcca tttgtcgaag acattgcaaa tcaggttgat acgggtgcca agttggtcga
     4381 gttggttcat aattggtctt gatattgact aaatttgtgc g
//
LOCUS       DAXHNE010000148         4410 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.148, whole genome shotgun sequence.
ACCESSION   DAXHNE010000148 DAXHNE010000000
VERSION     DAXHNE010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.148"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(97..585)
                     /locus_tag="ACFM4Z_001784"
     CDS             complement(97..585)
                     /locus_tag="ACFM4Z_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021771.1"
                     /translation="MKKDIFYCEQWSYGYKKLHKPFSEKQAEEKHLKGELYTAVIGSA
                     TQPEYVITLREEVGFFSVHFFDKFGRDYLTHQFQKYSNSNYYFLSMAVWRDYITLESH
                     DLAEGYTYFFNENTDDCYVLKEDFINNERYEKTELYSQKDKVILFPKFGEYDLVLNPD
                     II"
     gene            complement(582..3083)
                     /locus_tag="ACFM4Z_001785"
     CDS             complement(582..3083)
                     /locus_tag="ACFM4Z_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6021772.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRILLSTTDISSENGK
                     IKLQSYGDQFYYAGQGELYTFDKRSYKTGKWYKLKHVTEIKEHKNAKADPVSLSASQG
                     IEIKSGGNIGAHATLFDAPRGSVKIEAGRGLVLYAVEDLNYDKLDTRTKRKFIGITYD
                     KVHDTTTHTMKTALPSRVVAESANLQSGWDAKLQGTQFETTLGGAAIRAGVGDQARAD
                     AKIILEGIKSSVRTETVSSSKSALWQKQAGRGSNIETLQLPSFTGSVAPVLSAPGGYI
                     VDIPKGNLKTQIETLTKQPEYAYLKQLQVAKNINWNQVQLAYDKWDYKQEGMTPAAAA
                     VVVIVVTVLTYGALSAPAAAGTAGAAGAGAGGAAAGTAAGTGVAAGTAATTGVAAGTS
                     AAAITTAAGKAALASLASQAAVSLINNKGDINHTLKELGKSSTVRQAATAAVTAGVLQ
                     GISGLNTQAAEAVSKHFHSPAAGKLTANLINSTAAASVHTAINGGSLKDNLGDAALGA
                     IVSTVHGEVASKIKFNLSEDYIAHKIAHAVAGCASAVANKGKCRDGAIGAAVGEMVGE
                     TLLDGRDVGKLSPQERQKVIAYSQIIAGSVVALVKGDVNTAANAATVAVENNSLLARR
                     RVNIRWTPRQELEHEYAILEIQAITNQIRRLDPKFNGIAILRTPGEPWTRHDVQTYRQ
                     YYNQLRESRGFAVDPIYRIRINNGNEFNRIMSSKYPYNELYVANPKSATGYFRVDSYN
                     PATEEIISRKFTQFSQIQESTGIGYIKEAVRKYSPGAVISNVPSTPTTIRGRKLEGKL
                     ILEVPAQVNPIPQSVLRAAQEENVIIRDTTGRIYK"
     gene            complement(3442..3816)
                     /locus_tag="ACFM4Z_001786"
     CDS             complement(3442..3816)
                     /locus_tag="ACFM4Z_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HFC6021773.1"
                     /translation="MDEKQKIKILDFQIDLSSIFNSYKNQMGINIQDKNLKNNFCSFM
                     EELLNDGSIRLHDYTDGIGIPLTGTSKEQVQKLKDIWPTLEDAQAIWPEDPWYYLEWL
                     WWDIACPIDLADLPNIDIYEQA"
     gene            complement(3835..>4410)
                     /locus_tag="ACFM4Z_001787"
     CDS             complement(3835..>4410)
                     /locus_tag="ACFM4Z_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021774.1"
                     /translation="EAGAGIATGAVTVGNAWEAPVGALSKAKAAKQAAPKETINNLAN
                     LAKAEQQILFRIAQRDTQLDAWKTGFNNRVRKGAGLLDASNIPITINGKTIKPVQAIS
                     LKGAPVYSGVSEQEIFALYRQMTGQNPNFRVLPDGRLANGIISTGEWAGTKIALRNFS
                     KTENSTQARWTLDLQNPPSFIKGTKLELKFQ"
BASE COUNT         1028 a         1026 c          971 g         1385 t
ORIGIN      
        1 ctgataattt cctggttttt atcagcttta tcctcagatt ccaaacccaa taataaaagc
       61 cgtttgaaat ttttttcaga cggccttaaa acttaattaa ataatgtccg gatttaacac
      121 caaatcatat tcaccaaact ttggaaatag aattacctta tctttttggg aatataattc
      181 tgttttttca tatcgctcat tattaataaa atcctctttc aaaacatagc aatcatccgt
      241 attttcattg aagaaataag tatatccttc tgctaagtca tgagattcca aagttatata
      301 atctctccat acagccatag aaagaaaata ataattcgaa ttggaatatt tttgaaattg
      361 atgggttaaa taatcccttc caaatttatc gaaaaaatgt accgaaaaaa aacctacttc
      421 ctctcgcaag gtaattacat attcaggttg tgtcgccgaa cctattacgg cagtatataa
      481 ctccccttta agatgttttt cctcagcttg tttctcagaa aaaggcttat gaagtttctt
      541 ataaccataa gaccactgct cacaataaaa aatatctttc ttcatttgta aatccttcct
      601 gttgtatctc taatgataac attttcttct tgtgccgccc ttaatacaga ttgtggaatt
      661 ggattgacct gagcaggaac ttctaaaata agttttcctt caagctttct tcctcttatc
      721 gtagtaggtg tacttggaac attggaaatg acagcaccag ggctatattt tctaacagcc
      781 tccttgatat aaccaatccc cgtactttct tggatttgag aaaattgggt aaattttctt
      841 gaaataattt cctctgtcgc aggattatac gaatctaccc taaaataccc cgtcgccgat
      901 ttaggattgg ctacataaag ctcattataa gggtattttg atgacatgat acggttaaat
      961 tcattgccgt tgtttatcct gattctataa attgggtcaa cagcaaagcc tctggattcc
     1021 cttaattgat tataatattg cctgtatgtt tgtacatcat gtcttgtcca cggctctcca
     1081 ggagtcctca gaatagcaat cccgttaaat ttcggatcca gccttcggat ttgattggta
     1141 atggcctgga tttcaagaat ggcatattca tgttccaatt cttgtcgcgg agtccaacgt
     1201 atatttaccc tcctgcgagc taaaagacta ttattctcca ctgccacagt agccgcattc
     1261 gccgccgtat tcacatcccc tttaaccaat gccactacgc tgcctgcgat aatctgcgag
     1321 taggctatga ctttttggcg ttcttggggt gacagtttgc ctacatcgcg tccgtccaac
     1381 agggtttctc ccaccatctc gccgactgcc gcgccgattg cgccgtcccg acatttgcct
     1441 ttatttgcta ccgccgatgc acagcctgct acggcatggg ctatcttgtg ggcaatgtag
     1501 tcttcgctga gattaaattt gattttgctc gctacttctc cgtgtacggt actgactatc
     1561 gcacccagtg cggcatcgcc caagttgtct tttaggctgc cgccgttgat ggcggtatgg
     1621 acacttgcgg cagcggtgct gttgatcagg ttagcggtca gtttgcctgc tgcgggactg
     1681 tgaaaatgtt tgctgacggc ttcggctgct tgggtgttca gcccgcttat gccctgcagt
     1741 acgcctgcgg ttacggcggc ggtggcggcc tgtctgacgg tgctgctttt gcccagttct
     1801 ttcagggtat ggtttatgtc tcctttgttg ttgatgaggg aaactgcggc ttggctggcg
     1861 agactggcca gtgcggcttt gcctgcggct gtggtgatag ctgcagctga tgtgcctgct
     1921 gctactccgg ttgtggctgc cgttcctgct gctactccag ttccggctgc cgttcctgct
     1981 gcggctcctc ccgctcctgc gcctgccgcg cccgccgttc cggcggctgc cggggcggac
     2041 agtgcgccgt aggtcaatac agttacgacg ataacgacga cagctgctgc tgcgggtgtc
     2101 atgccctcct gtttgtagtc ccatttatcg taagcaagct gcacctgatt ccagttgatg
     2161 tttttcgcaa cttgaagttg tttcaaataa gcatactcgg gctgcttggt gagggtttcg
     2221 atttgggttt tcagattgcc tttgggaata tcgacaatgt aaccgccggg tgcggacagt
     2281 acgggcgcaa cggagcctgt gaaacttggc agttgcaagg tttcgatatt gctgccgcgt
     2341 ccggcctgtt tctgccagag ggcagatttg ctactgctta ctgtttcagt gcgcacacta
     2401 cttttgatgc cttcaagaat aatcttggca tctgctcgtg cctgatcgcc tacacctgca
     2461 cggatggctg cgccgcccag cgtggtttca aactgggtgc cttgcagttt ggcgtcccag
     2521 cctgattgca ggttggccga ttctgcaact acccttgagg gcagggcggt tttcatggtg
     2581 tgggtggtgg tgtcgtgcac cttgtcgtag gtaatgccga taaatttgcg cttggtacgg
     2641 gtgtcaagtt tgtcgtagtt gagatcttcc acggcataga gaaccagccc acgtccggct
     2701 tcgattttaa cggagccgcg gggtgcatca aacaaggtgg cgtgggcacc gatattgccg
     2761 ccggatttga tttcaatacc ttgtgacgca ctgaggctca ccgggtcggc tttggcgttt
     2821 ttatgctctt tgatttcagt aacatgtttt agtttgtacc acttaccggt tttatagctg
     2881 cgtttatcga aggtgtagag ctcaccctgt ccggcgtagt agaactggtc gccgtaggat
     2941 tgcagtttga ttttgccgtt ttccgaactg atatccgtgg tgctcagcag gatgcggctg
     3001 ttttcattgg catacggcgc gctaatgctc acaccggttt tacccgaaag ttctgcagca
     3061 agcgtaggag gtgtcttcag cacgcacgcg tctgaacagt atacagagaa tctgaatatt
     3121 tacttgcata acaaatgccg tctgaaaaaa ttgagctttt cagacggcat tgagccgtaa
     3181 atcatggaac gcgtgcgcgc tgaagcacac accttacgca tggattttag gtttcatgca
     3241 ggctacagct tgcttccata aatcattttt atcagagctc gtaggtacgg ttagccgcct
     3301 ttagcggcgt aaccgtacga atgaaatgcc aagttgcaag gccgtctgaa aaagttgaaa
     3361 aacagatttc agacggcctt gttattttat aaagtttgct gatatgcgta cggttacgcc
     3421 gctaaaggcg gctaaccata cctacgcttg ctcataaata tcaatattcg gcaaatcggc
     3481 caaatctatt ggacacgcaa tatcccacca aagccattct aagtaatacc aagggtcttc
     3541 aggccatatt gcttgggcat cttccaaagt aggccatatg tctttcaatt tctgcacttg
     3601 ttcttttgaa gttccagtta gaggaattcc gataccgtcg gtataatcat gtaaacggat
     3661 tgaaccgtca tttaacagtt cttccataaa agaacagaaa ttgtttttta agtttttatc
     3721 ttgaatatta atacccattt gatttttata agagttaaaa attgaggata aatcgatttg
     3781 aaaatcaaga atcttaattt tttgtttctc atccacggta aaatcctttg taaattattg
     3841 gaatttaagc tccaatttag tacctttaat aaatgatgga ggattctgca aatctaatgt
     3901 ccatcgtgct tgagttgaat tctctgtttt tgaaaaattt cttaatgcaa tttttgttcc
     3961 tgcccattct ccagtactga taatgccatt tgctaatctt ccgtcaggca aaactctaaa
     4021 attcggattc tggccagtca tctgccgata aagcgcaaaa atctcctgtt cgcttacgcc
     4081 gctgtaaacg ggtgctccct ttaagcttat ggcttgtaca ggtttgatgg tttttccgtt
     4141 aatggttatc ggaatattac ttgcatcaag caagcctgct cctttcctta ctctattgtt
     4201 aaatcccgtc ttccatgcat ccagttgcgt atcgcgttgg gcaatacgga ataaaatctg
     4261 ctgttctgct ttggctaaat ttgccaaatt gtttattgtt tctttaggag cagcttgctt
     4321 agccgccttc gctttcgaca acgctcctac tggagcttcc caagcgttgc ctaccgttac
     4381 cgcacccgtg gcgattcccg cgcccgcttc
//
LOCUS       DAXHNE010000149         4365 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.149, whole genome shotgun sequence.
ACCESSION   DAXHNE010000149 DAXHNE010000000
VERSION     DAXHNE010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.149"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(69..2045)
                     /locus_tag="ACFM4Z_001788"
     CDS             complement(69..2045)
                     /locus_tag="ACFM4Z_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFC6021775.1"
                     /translation="MNEFSLAPIVIVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGITMLSVM
                     GILMLTGVPFNWAFSVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2194..3033
                     /locus_tag="ACFM4Z_001789"
     CDS             2194..3033
                     /locus_tag="ACFM4Z_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFC6021776.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVIRDISALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3233..4264
                     /gene="gap"
                     /locus_tag="ACFM4Z_001790"
     CDS             3233..4264
                     /gene="gap"
                     /locus_tag="ACFM4Z_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC6021777.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGKLDGLAIRVPTINVSLVDLSFQAARDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
BASE COUNT         1110 a         1462 c         1013 g          780 t
ORIGIN      
        1 tgcgatttgc gaaagcgtta ttaaaccaaa taaccggaaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caaactccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgccttcc aatgtaaacg aggcatccgg gttttcaatc
      181 cggccggtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg acggacggtt
      301 tcgccgtttt cgccgaatcc tgcctcatca tcactaccga caaacagtcc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccgata cgacgcgccg
      421 ttgcctaaca ttgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ctatatcggt aaacgttttc acataatcgt cgttggtggc gcgcacatat
      541 acgggcatat tgggatacat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg taaccaccac cattttcgcc cgtcccagac cggcggcttc caatacttcc
      661 ctgcgtttcg catcgccgaa cgacaccggt tcgcccgcac ttctggcaac ctgcacccgc
      721 gcaatgtcca agtcgagcgc gaaatacgga atatcctctt gggcaaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatggt cggacttgct catggtttct
      841 accagcatac tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caaacgcccg
      901 accagcgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagaaccgcc
      961 gccgtcgccg cctgttccca ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccctgcgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgtt tcattttgaa ggcaatggca aacacaacca gtgccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccag ccgccgatca atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa aaagccgagc aaaatatcgc ggaacgggcg gatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcagcatgc cggcaacgaa tgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgcccgcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaatt ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg acacggaaaa cgcccaattg aacggcacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaat gccgacctgc aaaccgccca gaccgaacac cagccgcctc
     1801 atcgccctca acttgggcag cgagaactcc aaaccgatgc tgaacatcag gaacacaatc
     1861 ccgatttcgc ccaaataatc cgtcgcatgg cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaaa acaatcacaa taggggcaag cgaaaattcg
     2041 ttcatagacc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgtattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgctaaa
     2161 atacgccctt tcgattttga gccgcacacc gacatgaccg ccacagccgc cgccatagac
     2221 cgcctgcttc cccaaaccca atgccgcgaa tgcggctacg acggctgcct gccctacgca
     2281 caggcagtcg caacaggcga agcgtacaac ctctgcgccc cgggcggaga agccgtcatt
     2341 cgggacattt ccgccctgct cggcaaaccc cttgtcgcac ctgccaaaac ccaagccaaa
     2401 gcgctcgccc ggatagacga aaccgcctgt atcggctgca ccgcctgcat ccgcgcctgc
     2461 cctgccgatg ccattatggg tgcgggcaaa cttatgcaca ccgtcatcgc cgacgaatgc
     2521 accggctgcg gactctgcgt cgccccctgc cccgtcgact gcatccatat gcagcccgtt
     2581 gccgacaccg tcctgccccg cgcgcgccgc ttcagcctgt ccgccgacag ccgttttgcc
     2641 gccgccgaac acgcgcgtgc gcgctacctc aaacgcaacg aacgcaaaca gcgcgaagcc
     2701 gacgaacgca aggccatgct tgccgaacgc gaagccgccg tccgcaacgc gcgtccgcaa
     2761 acacccgaca caccgaaaaa accggcgttt aaccctgccg acctcatcgc caaagccatg
     2821 gcaaaagcgc aaacccaaca agaccgcctc gccgccgccg acaaccgcaa agactatcag
     2881 gcgaaacaga tagccgaagc ccgcgaacgc gccgagttgc gccgcgccca acgcgatatg
     2941 aaatacggca gcgacagcga aaaagccgcc gccctcgaat acctcaaaca atacaaagcc
     3001 aaacaggaag ccgcacagaa taccgcctcc tgacccttcc ctgatatgcc gtctgaagcc
     3061 gcttcagacg gcattttatc aagctctccg tccgccgcac ccgtgccgtc cgcatcttac
     3121 cgcccaccct tccggccgcg ccgttttcaa taaaatatta attacacgcc actacaaatt
     3181 tgctataatc cgccccgaaa atctacccaa cccctcaaca aaggaacgaa ccatgggcat
     3241 caaagtcgcc atcaacggct acggacgcat cggccgccag gttttgcgcg ccatctacga
     3301 ttatcagatt caagaccaac tccaaatcgt cgccgtcaac gccagcggca gccttgaaac
     3361 caacgcccat ctgaccaaat tcgacaccgt acacggacgc tttgaagccg acgtatccca
     3421 cgacggcggc aacctcatcg tcaacggcga caaaatcccc ttcttctcga cccgcaaccc
     3481 tgccgaactg ccgtggaaag aactcggtgt cgatttggtc atggaatgca ccggcgcgtt
     3541 caccagcaaa gaaaaagcca aagttcatct ggaaagcggc gcaaaaaaag tcctcatttc
     3601 cgcaccgggc ggcgacgatg tcgatgcaac cgtcgtgtac ggcgtgaacg acagcgtcct
     3661 gaccgccgac atgaccgtcg tttccaacgc ttcctgcacc accaactgcc tctcgccggt
     3721 tgccaaagtg ttgagcgaaa gcgtcggcat cgtcaaaggc gcgatgacca ccatccacgc
     3781 gctgaccaac gaccaaaccg ttaccgacgt gcgccacaaa gacctgcgcc gcgcccgcag
     3841 cggcgtggaa aacatgattc cgaccaaaac cggcgcggca aaagccgtcg gactggtact
     3901 gcccgaattg aaaggcaaac tcgacggact cgccatccgc gtgccgacca tcaacgtatc
     3961 attggtagat ttgagcttcc aagccgcgcg cgacaccaca gtcgaagaaa tcaacgcact
     4021 gatgaaagcc gcctcggaag cgggtgcgct caaaggcgtt ttaggctaca acaccctgcc
     4081 gcttgtttct atggacttca accacaccac cgaagccagc cacttcgacg ccacactgac
     4141 caaagtcgtt gacggcaaca tgattaaagt cttcgcatgg tatgacaacg aatggggctt
     4201 cagctgccaa atgctgaaca ccgcacgccg catgttcgga cttgaagtgc gcccgctcaa
     4261 ataagcaaca aaccgtcaaa caaaatgccg tctgaaaccc gatgttttca agtttcagac
     4321 ggcatttttc accgtgattt tacccggctg tcgtcatttc caatt
//
LOCUS       DAXHNE010000150         4258 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.150, whole genome shotgun sequence.
ACCESSION   DAXHNE010000150 DAXHNE010000000
VERSION     DAXHNE010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.150"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            536..2263
                     /gene="ilvB"
                     /locus_tag="ACFM4Z_001791"
     CDS             536..2263
                     /gene="ilvB"
                     /locus_tag="ACFM4Z_001791"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFC6021778.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSSDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKVDIPIVGDVKNVLSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSQDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADGKA"
     gene            2274..2765
                     /gene="ilvN"
                     /locus_tag="ACFM4Z_001792"
     CDS             2274..2765
                     /gene="ilvN"
                     /locus_tag="ACFM4Z_001792"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFC6021779.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            2832..3125
                     /locus_tag="ACFM4Z_001793"
     CDS             2832..3125
                     /locus_tag="ACFM4Z_001793"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFC6021780.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            3205..4218
                     /gene="ilvC"
                     /locus_tag="ACFM4Z_001794"
     CDS             3205..4218
                     /gene="ilvC"
                     /locus_tag="ACFM4Z_001794"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFC6021781.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGYVVKTVAEATKEADVVMLLLPDETMPAVYHAEVTANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
BASE COUNT         1111 a         1018 c         1075 g         1054 t
ORIGIN      
        1 caatatatta agtcgttgtt agccctcttg gttctgtcaa tgccgtccaa tacagtccgg
       61 tgcaatgagc cattttcagg aagtatggtt gggaatattt ttgatatatc aaaaataaaa
      121 tcaagttcaa tcaatatccc gagtctttat tgggatgaaa ccctgtaaat tttacaattc
      181 ttgatatcga agaagggttt ttcacatctg cccctcctac acaaactgtc aacaatttcc
      241 atctttcaaa aaaggtactc ctgattttat tcttttaaaa tcaatcaata atttctctgc
      301 tttcaaaaaa tattaaaaga tgaaagattt ttcttgattg aactgtcggt ttgcgttatc
      361 gtttctactc tttttcacaa actctgtgtt cctttccaat tgattggata atccgtctgt
      421 tgccgtgttc ccccaatgcg cgtaccctaa atcgctgctt ggtggaattg cattacaggt
      481 gctgtggcaa agcggtttgt ccctattggt ttgaaaccgt ataaaagagg tcgttatgca
      541 gttatcaggc gcgcaaatca tagtgcagag tctcaaagcc gaaggtgtcg agtacgtttt
      601 cggttatccc ggcggtgcgg ttatcgaaat ctacgatgcc cttttccaac tcaataaatt
      661 caagcacatt ctgacccgtc acgagcaggc ggcagtacac gcggcagatg cgtatgcgcg
      721 cgtcagcggt aaggtgggcg tggcattggt tacatccggc ccgggcgtta ccaatgcact
      781 gaccggtatt gctactgcct atacggattc gattccgatg gtggtcatca gcgggcaggt
      841 aggcaattcc ctgattggta cggatgcgtt ccaagaagtt gatacggtgg gtattacccg
      901 tccgtgcgtc aaacacaatt tcctggttac ggatatcaat gagttggcgg aaaccattaa
      961 aaaggcgttc caaattgccg caagcggccg accggggcct gtggtggttg atgtcccgaa
     1021 agatgttacg caggcgatgg cgaaattcag ctatcctcag gaagacattt ttatccgttc
     1081 gtatcaaccg gttgttcaag ggcatatcgg tcagattaaa aaggccgtgc agatgttggc
     1141 atctgccaaa cgtccagtcg tctattttgg cggcggcgtg gtgttgggta atgcttctga
     1201 agagctgacc cgatttgtcc gaatgacggg tgctccgtgc acgggtacgc tgatgggctt
     1261 gggcgcttat ccttccagcg accgccaatt cctcggtatg ctcggtatgc acggtactta
     1321 cgaggcaaac cttgccatgc agaatgcgga tgtcgttctt gccgtaggtg cgcgttttga
     1381 cgaccgtgtg gtttccgtac cgtccaaatt tttcgagaag gcgaagaagg ttatccatat
     1441 cgatgtcgat ccttccagca tcgccaaacg cgtgaaggtg gacattccga ttgtcggcga
     1501 cgtgaaaaac gttttgtccg agatggttgc gctgtggcaa aaacaagagt ccgtgccgtc
     1561 tgaagatgct ttgggcaaat ggtggaaaac catagaggaa tggcgttccc aagattgctt
     1621 gtggtttgac aacggcagcg aaattatcaa gccccaatat gtgattcaga agcttgccga
     1681 gattaccggc aattcggcaa tcatcacatc ggatgtaggg cagcatcaaa tgtttgcggc
     1741 tcaatattat cccttcgaac gtccgcgcca atggctcaat tccggcggtt tgggtacgat
     1801 gggcgtgggt ctgccttatg cgattggtgc aaaacttgcc gccccggatc aagacgtatt
     1861 ctgtatcacc ggcgacggct cgatccagat gaacatccaa gagctgtcca cctgtttcca
     1921 atatcggatt ccggttaacg tcattacgct gaacaacggt tatctcggta tggtacgcca
     1981 gtggcaggaa atatattacg gcggacgaga gtcggaaacc tatttcgatt ctttgcccga
     2041 tttcgtcaaa cttgccgagg catacggcca tatcggtatc cgcgtggaca agaagtctga
     2101 tgtggaaggt gcgttgttgg aagcattgaa ccaaaaagac aggctggtgt ttatcgactt
     2161 cctgaccgac cagaaacaga atgtgatgcc catggtcggc aacggcaaag gtttggacga
     2221 aatggtactt ccgccgcata tgcgtgcgga cggaaaggcg taaggagaga caaatgcgac
     2281 atatcttatc tgttctgatt gaaaacgaat caggtgcgat gagccgcgtg gtcggtttgt
     2341 tctctgcacg cgattacaat atcgattctt tggcggttgc gccgaccgaa gacaaaaccc
     2401 tgtcacggat gaccatcgtt acccacggcg acgagcaggt catcgaacaa attaccaagc
     2461 aactcaataa attgattgaa gtgattaaag tggtcgattt gaatgaaagc cgttttgtcg
     2521 aacgcgaact gatgttggta aaagtccgtg ccgccggcaa agaccgcgac gaatttttac
     2581 gcttgaccga aatctaccgg ggcagcatca tcgacgtaac cgaccgcagc tatacgattg
     2641 aaattaccgg ctcgacagac aagctggatt cctttttgga aacggtcgga cgcgcccaaa
     2701 ttttggaaac cgtacgcaca ggcgcggccg gcatcggtcg cggtgagcgt attttgaaaa
     2761 tttaacgccg taacctttca gacggcacgg tatttgaatg tcgtctgaaa aacgaacggc
     2821 aggagagatt tatgtcgaac attaaaattg tcgcactggt taccgtcaaa ccggaataca
     2881 cagaaacact ggcagcacag tttaaagaac tggtcaaagc cagccgtgca gaagagggca
     2941 acatcagcta cgatctccat caggaaatcg gcaaaccgaa ccgttttgtt ttcgtggaaa
     3001 attggaaatc tcaagcagct attgacgaac ataacgccag cgcgcatttc caagccttcg
     3061 tccagtccgt cgacggcaaa accgaagcgt tggaaatcgt attgatgaat gaagttgccg
     3121 attaagcggc acactctgtt taacccgtcc gaagccgctc aacattttaa ggcttcgaca
     3181 accatttacc ttaaaggaaa tcaaatgcaa gtctattacg ataaagatgc cgatctgtcc
     3241 ctaatcaaag gcaaaaccgt tgccatcatc ggttacggtt cgcaaggtca tgcccatgcc
     3301 gccaacctga aagattcggg tgtaaacgtg gtgattggtc tgcgccaagg ttcttcttgg
     3361 aaaaaagccg aagcagccgg ttatgtcgtc aaaaccgttg ctgaagcgac caaagaagcc
     3421 gatgtcgtta tgctgctgct gcctgacgaa accatgcctg ccgtctatca cgccgaagtt
     3481 acagccaatt tgaaagaagg cgcaacgctg gcatttgcac acggcttcaa cgtgcactac
     3541 aaccaaatcg ttccgcgtgc cgacttggac gtgattatgg ttgcccccaa aggtccgggc
     3601 cataccgtac gcagtgaata caaacgcggc ggcggcgtgc cttctctgat tgccgtttac
     3661 caagacaatt ccggcaaagc caaagacatc gccctgtctt atgcggctgc caacggcggc
     3721 accaaaggcg gtgtgattga aaccactttc cgcgaagaaa ccgaaaccga tctgttcggc
     3781 gaacaagccg tattgtgcgg cggcgtggtc gagttgatca aggcgggttt tgaaaccctg
     3841 accgaagccg gttacgcgcc tgaaatggct tacttcgaat gtctgcacga aatgaaactg
     3901 atcgttgacc tgattttcga aggcggtatt gccaatatga actactccat ttccaacaat
     3961 gcggagtacg gcgaatacgt taccggccct gaagtggtca atgcttccag caaagaagcc
     4021 atgcgcaatg ccctgaaacg cattcaaacc ggcgaatacg caaaaatgtt tatccaagag
     4081 ggtaatgtca actatgcgtc tatgactgcc cgccgccgtc tgaatgccga ccaccaagtt
     4141 gaaaaagtcg gcgcacaact gcgtgccatg atgccttgga ttactgccaa caaattggtt
     4201 gaccaagaca aaaactgatt gttttcaaac gggactacct acacatcgtg taggcagt
//
LOCUS       DAXHNE010000151         4220 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.151, whole genome shotgun sequence.
ACCESSION   DAXHNE010000151 DAXHNE010000000
VERSION     DAXHNE010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.151"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(82..1128)
                     /gene="recA"
                     /locus_tag="ACFM4Z_001795"
     CDS             complement(82..1128)
                     /gene="recA"
                     /locus_tag="ACFM4Z_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFC6021782.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEVANEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            1382..2146
                     /gene="aroD"
                     /locus_tag="ACFM4Z_001796"
     CDS             1382..2146
                     /gene="aroD"
                     /locus_tag="ACFM4Z_001796"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFC6021783.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLDQIKNLPFD
                     IVEFRADFLECAGSIGEVLRHTQTVRDALPDKPLLFTFRRHGEGGAFPCSDDYYFKLL
                     DALIESRLPDIIDIELFAGETAVRRAVADAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLEAKRLAAKPVVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLESGAD"
     gene            2166..4181
                     /gene="rep"
                     /locus_tag="ACFM4Z_001797"
     CDS             2166..4181
                     /gene="rep"
                     /locus_tag="ACFM4Z_001797"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFC6021784.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKYLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKSQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRHKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHKHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLLASEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDSMKKGGPED"
BASE COUNT          992 a         1155 c         1166 g          907 t
ORIGIN      
        1 aaactcaaat caagccgttc ggaggcggct caaaaaaacg gtacttcgca gcagaagtac
       61 cgtttatcgg gatttcaggt tttattcttc ggggcgttcg ccgtcggttt cgtcctgcgt
      121 cccttcggtg atgtgcattt ctacgccgtt gagggcgcgg atttttgcgt cgatttcatt
      181 ggcgacttcg ggattttcct tcagccagac gcggacgttg tctttgccct gaccgatttt
      241 cgcgccgttg tagctgtacc acgcgccgga tttgttgatg atgtcgtttt tcacgccgat
      301 gtcgatcaat tcgccttccc aactgatgcc ttctccgtag aggatgtcaa actctgcctg
      361 acggaacggg ggggcgactt tgtttttgat gactttgacg cgggtttcgt tgcccaatac
      421 ttcttcgcct tttttgatgg atccggtgcg gcggatgtcg aggcggacgg aagaatagaa
      481 tttcagcgcg ttgccgccgg tggtggtctc ggggctgccg aacattacgc cgatcttcat
      541 ccggatttgg ttgatgaaca caaccagcgt gttggttttt ttgatgtgtc cggtcagttt
      601 gcgcaaagcc tgactcatca ggcgcgcctg cagtccgaca tggctgtccc ccatatcgcc
      661 ttcgatttcg gctttgggga cgagtgcggc tacggaatcg acgactacca tatctatgcc
      721 gcccgaacgg acgagtgtgt cgcagatttc caaagcctgt tcgccggtat cgggctggga
      781 caggtaaagc tcttcgactt ttacgccgag tttgcgggcg taaacgggat caaaggcgtg
      841 ttcggcatcg acaaaggcgc acacgccgcc gtttttctgg cattgggcga cggcttcgag
      901 gcagagggtg gttttgccgg aggattcggg gccgaagatt tcgacgatgc gcccgcgcgg
      961 cagaccgccg actccgaggg cgaggtctaa tccgagcgat ccggtggaaa tgacttcgag
     1021 gttttcttcc tgctggctgc cgtccatttt catgatggcg cctttgccga aacttttttc
     1081 gatttgcgcc agtgcggcgg caagggcttt gcttttgtcg tctgacattg gggttactcc
     1141 ggaacaaatg cggtatgtag gatgcggcgc aacacgggct gcggcgcggg atgtgtatcg
     1201 ttttcccgat gtgcgggcta tcggtaatgc tgcttcacga ggttgccatt atcgcatatt
     1261 tccttgcttg ccgatatgcg gcaggacgcg gcggcttgtg ccggaatgga atctggatgc
     1321 cgtctaaaag gcggccggct ttgttataat ggcggctgtt ttttctgtgt gtgcctgttt
     1381 tatgtgttcc tgccttgttg tcaaaaatac cgttatcgga agcggacgca ccaaaatcgc
     1441 cgtgccgctt gtcgcccgcg atgccgccga actttccgcc gtacttgacc aaatcaaaaa
     1501 cctgcccttc gacatcgtcg agttccgcgc cgattttttg gaatgcgcgg gcagcatcgg
     1561 cgaagtattg cgccacacgc agaccgtccg cgacgcgctg cccgacaagc cgctgctgtt
     1621 tacgttcaga cggcatggcg aaggcggcgc gttcccgtgt tcggacgatt attatttcaa
     1681 actgctcgac gcgctgatcg aaagccgcct gcccgacatc atcgacatcg agctgtttgc
     1741 cggcgaaacc gccgtccggc gcgccgtggc ggatgctcaa aaaaacggca tcgccgccct
     1801 gctctgcaat catgagtttc accgcacgcc gccgcaagaa gaaatcgtat gccgtctgaa
     1861 acagatggag gactgcggcg cggacatctg caaaattgcg gtgatgccgc aaagcgcgga
     1921 agatgtgctg actttgcttt ccgccacgct cgaagcgaaa cggcttgccg ccaaaccggt
     1981 tgttacgatg tcgatggggc agacgggtgc ggtcagccgc cttgccggac aggtgttcgg
     2041 ctcaagcatc acgttcggtt cgggaacgca aaactccgcg ccggggcaaa tcggcgtatc
     2101 cgccctccgt gcggcactcg actgtctcga aagcggcgca gactgatttc agacggcatc
     2161 aaaacatgat gaaactcaat ccccaacagc tcgaagccgt ccgttacctc ggcggtcccc
     2221 tgctcgtcct cgccggcgct ggcagcggca aaaccggcgt gattacgcaa aaaatcaagt
     2281 atttgattgt caatgtcggc tatctgccgc ataccgttgc cgcgattacc tttaccaata
     2341 aagccgccgc ggaaatgcag gagcgcgtcg ccaaaatgct gcccaagtcg caaacgcgcg
     2401 ggctgacgat ttgcacgttc cattctttgg gcatgaagat tttgcgcgaa gaggcgaacc
     2461 atatcggtta caaaaaaaac ttctccattc tcgattctac cgacagcgcg aaaatcatcg
     2521 gcgaactctt aggcggtacg ggcaaagaag ccgtattcaa ggcgcagcac cagatttcct
     2581 tgtggaaaaa cgatttaaaa acgcctgaag atgtcgttca gacggcatcg aacatttggg
     2641 aacaacaaac cgcacgcgtg tatgcgagct atcaggaaac cttacaaagc tatcaggcag
     2701 tggactttga cgacttaatc cgcctgcctg ccgtgctgtt gcagcaaaac agcgaagtgc
     2761 gccacaaatg gcagcggcgg ctgcgttatc tgctggttga cgaatgccaa gatacgaata
     2821 cctgccaatt tacgttgatg aaacttctga ccggcgcgga aggtatgttt accgccgtcg
     2881 gcgacgacga ccagtccatc tacgcatggc gcggtgcgaa catggaaaac ctgcgtaaaa
     2941 tgcaggaaaa ctatccgcag atgaaggtca tcaaactgga gcaaaactac cgctccaccg
     3001 cgcggattct caaaatcgcc aacaaagtca tcgaaaacaa ccccaagctg tttaccaaaa
     3061 aactttggtc gcaattgggc gaaggcgagc cggtcaaagt cgttgcctgc caaaacgagc
     3121 aacacgaagc cgactgggtc gtcagccaaa tcgtcaaaca aaaactcatc ggcggcgaca
     3181 aaacccaata tgccgatttc gccgtgttgt accggggaaa gcatcaggcg aggattttcg
     3241 aggaagcatt gcgcggcgcg cgcatcccct accgtctctc cggcggacaa agctttttcg
     3301 acaaagccga aatcaaagac gtgttgtctt atgtgcggct gcttgccaac cccaacgacg
     3361 atcccgcctt tctgcgtgcc gttaccacgc ccaaacgcgg catcggcgat gtaacgctgg
     3421 gcaagctcaa cacttacgcg cacaaacacg aatgcagcct gtatgaagcc gcgcaaaacg
     3481 aagaagccct cgccacgctg aacaatacca accgccaaca cctgcaagcc tttatggata
     3541 tgttcggcag ttaccgtgcc aaagccgaaa ccggcgaagc gggcgagttc atcaacagcc
     3601 tgctcgaaga aatcgactat gaaaaccacc tgcttgccag tgaagaaggc aaagctggcg
     3661 aaatcaaatg gcgcaacgtc ggcgatttgg tatcatggtt tgcgcgaaaa ggcggggaag
     3721 acggcaaaaa catcatcgaa ctcgcccaaa ccgtcgcctt gatgacgctt ttggaaggaa
     3781 aagacgaaga agaaaccgat gccgtctcgc tatccacgct acacgccgcc aaaggtttgg
     3841 agtatccgta tgttttcctt gtcggttgcg aagaaggcgt tttgccgcac aacgacagca
     3901 tcgaagaagg caacgtcgaa gaagaacgcc gcctgatgta cgtcggcatc acccgcgcca
     3961 aacgccaact cacactgacc cactgcgtca aacgcaaaaa acaaggcaca tggcagttcc
     4021 ccgaacccag ccgattcata gacgaaatgc cgcaggaaga tttgaaaatc ctgggtcgca
     4081 aaggcggcga accgattgtc agcaaagaag aaggcagacg caaccttgcc gatataatcg
     4141 gaaggctcga cagcatgaaa aaaggcggtc cggaggatta aaccaagata gggaaacgaa
     4201 gccggagaca atgtatagtg
//
LOCUS       DAXHNE010000152         4203 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.152, whole genome shotgun sequence.
ACCESSION   DAXHNE010000152 DAXHNE010000000
VERSION     DAXHNE010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.152"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(1..143)
                     /locus_tag="ACFM4Z_001798"
                     /pseudo
     CDS             complement(1..143)
                     /locus_tag="ACFM4Z_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(271..1521)
                     /gene="glyA"
                     /locus_tag="ACFM4Z_001799"
     CDS             complement(271..1521)
                     /gene="glyA"
                     /locus_tag="ACFM4Z_001799"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFC6021785.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVRKQVTALCNKYPVYGA"
     gene            complement(1676..1939)
                     /locus_tag="ACFM4Z_001800"
     CDS             complement(1676..1939)
                     /locus_tag="ACFM4Z_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021786.1"
                     /translation="MSNQTKIQAIKQASEQIFAICETPNTALQAIHLILRHGGAGELS
                     WQVVYQRVMADKDVVGAGYLIDFAQTAENLPFDALPLGSLALT"
     gene            complement(2002..3777)
                     /gene="ggt"
                     /locus_tag="ACFM4Z_001801"
     CDS             complement(2002..3777)
                     /gene="ggt"
                     /locus_tag="ACFM4Z_001801"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFC6021787.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGSYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(3945..>4203)
                     /locus_tag="ACFM4Z_001802"
     CDS             complement(3945..>4203)
                     /locus_tag="ACFM4Z_001802"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HFC6021788.1"
                     /translation="RHAPVSPMWIFSACSRGADPIGRPFAFVNILENPEIRSTLPLIA
                     SRPTFPQLWVNGELIGGSDIILQMYQSGELKPLIEEHSPKV"
BASE COUNT         1042 a          988 c         1007 g         1166 t
ORIGIN      
        1 atctctgagg taacgggttt ttgacggttc aggtatggtt cgatcggctg ccaatcaatc
       61 acccggtcca acttcaatag cgggaaacgg tcgatgtgtt tggcaattat ggcttgtgcg
      121 gtttgccgga agaaggtgct catgagaaat cccctaaatg tcttggtggg aatttagggg
      181 attttgggga tttttgcaaa ggtttccgcc tgaaacatta tgagatttca ggcggcattg
      241 gattgcttgg cggaatattt ttaaaaaggc ttacgcgccg taaacggggt atttattgca
      301 caaagcagtt acttgtttgc ggactttggc gaggttggct tcgtcttcgg ggttagacaa
      361 tacgtcggca accaagttcg ccaatacgcg tgcgtcggct tcgttaaaac cgcgtgtggt
      421 catggcagcg gagccgatgc ggatgccgga ggtaacgaag ggtttttccg gatcgttcgg
      481 aatggcgttt ttgttgacgg tgatgtgcgc tttgcccaaa gcggcttcgg cggctttgcc
      541 ggtaattttc atcggttgca ggtcgacgag gaaaacgtgg ctttcggtgc ggccggaaac
      601 gatgcgcaaa ccgcgtttga ccagttcttc cgccatggcg gcagcattga ttttcacttg
      661 ttttgcgtat tgtttgaact cgggttgcaa cgcttcttta aacgctacgg ctttggcggc
      721 gatgacgtgc atcagcggac cgccttgcag gcttgggaag atggaagagt tcagtgcttt
      781 ttcgtgggta ttgtcgcggc acaaaattac gccgccgcga ggaccgcgta gggttttgtg
      841 ggtggtggtg gtcacgaagt cgcagaatgg cacggggttg ggatattcgc cgccggcaac
      901 cagaccggca tagtgcgcca tatcgacaaa gaggtatgcg ccgactttat cggcgatttc
      961 gcggaatttt gcccagtcga tttgcaacgc gtaggcagac gcacccgcca caatcatttt
     1021 gggtttgtgt tcgagagcca agcgttccac ttcggcataa tcgagcactt cgttttcatc
     1081 caaaccataa gtaacggcat tgtagagttt gccggaaata ttaacgctcg cgccgtgggt
     1141 caggtggccg ccgtgcgcca aagacatacc caaaatggtg tcgcccggtt ttaaaacgga
     1201 agcgtacacg gcttggttgg cttgcgagcc ggagtgcggt tgaacgttgg cgtattgcgc
     1261 gccgaacagt tttttgacgc ggtcgattgc caattgttcg acaatatcga cgtattcgca
     1321 gccgccgtag tagcgtttgc cggggtagcc ttcggcgtat ttgttggtca gctgggaacc
     1381 ttgcgcttcc attacggcgc agctgacgta gttttcagag gcaatcagct cgacgtggtc
     1441 ttgctggcgt tggtcttctt gggcaatggc tgctgccaaa tcggggtcat attgtgcgag
     1501 ggtaacgctt tttgaaaaca tgttctcggc tcctttgtgt aatcagggta tcatgagtgt
     1561 tttttgtata aaaaaatact tcaaaaccta aggcagatag tccataatgc gtaaattttc
     1621 tttggcatta tcaggtaatt tatttaacat gctggttttt agcgtctcat tacctttatg
     1681 ttagtgcaag gctacccaaa ggcaatgcat caaatggtaa attttcggca gtttgtgcaa
     1741 aatcaatcaa ataaccagca ccaaccacat ctttatcagc cataacccgc tgatacacca
     1801 cttgccaaga caactctcct gccccaccat gtcttaaaat cagatgaatg gcttgtaatg
     1861 ctgtgtttgg cgtctcacaa attgcgaaga tctgctcaga agcttgcttg atggcttgta
     1921 tttttgtttg attactcaca atttccccct attttaatat taacttaatg cggtcaaatt
     1981 cacaaaatac aagcttcacc tctaatcacc catcactcga ccttctcggc gtggatcggc
     2041 accaccaacc agcctgcttg gctcgataat aatggcttga acacctgaat ttagctcacg
     2101 cacgtcagtc ttatagccca aatcatttaa tgcttgttgc cactggaggg cggttgtacc
     2161 cgtttctagt tcatagctac caaagcgatt taataaattg ggtgcactga tggcatcttg
     2221 gatatccata ttccagtcac tatgtgccac aatcgtctta gcgacatagc caatgatacg
     2281 gctaccacct ggggagccga ttgccatata aggcttgcct gctttaaata cgatggttgg
     2341 tgccattgag gagcgtggtc tcttgccggg ctcgacacga ttggcgacct gtttgccctg
     2401 ctttattggc tcaaaactaa agtctgtcag ctcattattc agcaggtagc catttgccat
     2461 caaagttgag ccaaacgcat tttcaatgga agtcgtcatt gatagcacat tgcccgcctt
     2521 atccacaatt gatatatgac tggtagaagg taactcaatc gcttgtgagg acacccactc
     2581 atgaataaaa tcgcctgcag atacgctagg caatgcctta tccgactgct caagcagctg
     2641 gctgcgatgt tttaggtagt ctttagaaat caactggcga atgggtactg gtacaaaatc
     2701 agggtcgccc aaatatacat cacgatccgc aaacgcaagc ctagaagcgt cgcccaagag
     2761 acgtaaacct tcagcatcat accccacctg attgggtgaa aattcattta aaatccccaa
     2821 aatctgaccc acagcaatcc cacctgagct tggtgcaccc ataccgcata cttcataaat
     2881 acgataagtc acacaaacag gcgggcgttc caccacttga taatcagata aatcttgtaa
     2941 ggataattga ccggggttat ccttagcatt ttggacaact gaaacgatat tttgggcata
     3001 tttaccagta tgcagagctt ttgcaccttg agctgctaac gcctgaacac tgtcagcaaa
     3061 ttctaaattt ttcagcaagc tgcctgcttg tagcggcaca ccattcggca aaaaataagc
     3121 ggctgttttt ggatagcgtg ccaaatgctg ctgattttgc tcaaccgaga tggcaagcct
     3181 tggcgacacc tcaaagcctt gttttgccaa gcggatcggt gtatcaaata attttcccca
     3241 aggcaataca ccgtatcgct gatgtattgt ctccatcagt ttagggatag caggcgtacc
     3301 caccgagcga ccaccgacca ccgcttccat aaatttcaat ggttgaccat ctttatccaa
     3361 aaataattcc ggcgtcgcac gcatcggtgc cgtctcacgc ccatcaaatg tggtcaatgt
     3421 tttggcggta ttatcccaat acaacacaaa tgcaccaccg cccaagcctg acgactgtgg
     3481 ctctaccaag cttagtgtcg tctgcaccgc caccatcgca tctgcagcgc taccgccttg
     3541 ctttaagata tcatagccag cttgtgttgc taatggattg gctgacgcta ccataaaatc
     3601 acttgcaatc acctgctttt gttcggtcag tcccgttgca tgttcaggcg tgtgagcgtc
     3661 tgcacctgtg atgacagcag aatgagtatt aaccttacct tgattggcat ggatgacttg
     3721 acatccagag attgtcatag acattatcaa tgcagtcaat aaatatgttt tagccacaag
     3781 cactccttcg cctgagtttg attgatgatt catacaaggc atgctgatta ttgtatttaa
     3841 tatggctaaa taattcaatc caaactatca atcttgacca tcaaaaaaag accgctaatg
     3901 tcatcagcag tcttttttga tatttatttt aagatattaa gtaatcagac ctttgggcta
     3961 tgctcttcaa tgagtggttt tagctcacct gattggtaca tttgtaggat aatatcacta
     4021 ccaccgatta actcaccatt aacccaaagc tgtggaaagg ttggccgact ggcgatgagt
     4081 ggtagagtac tgcgaatttc tgggttttct aggatattaa caaaagcaaa gggtctgcca
     4141 attgggtcag cacctctact gcacgcgctg aaaatccaca ttggggaaac tggggcgtgc
     4201 ctt
//
LOCUS       DAXHNE010000153         4119 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.153, whole genome shotgun sequence.
ACCESSION   DAXHNE010000153 DAXHNE010000000
VERSION     DAXHNE010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.153"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            43..675
                     /locus_tag="ACFM4Z_001803"
     CDS             43..675
                     /locus_tag="ACFM4Z_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFC6021789.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAVSLFAISDDEDEIVAYLSEHGLQTA"
     gene            747..2117
                     /gene="purB"
                     /locus_tag="ACFM4Z_001804"
     CDS             747..2117
                     /gene="purB"
                     /locus_tag="ACFM4Z_001804"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFC6021790.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKER
                     FSGVPEVAAASEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTGMAHEL
                     AAVPMMSRTHGQPATPSTLGKEIANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQAISRVNTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKDGITPEVLKLFIESLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            2197..4065
                     /locus_tag="ACFM4Z_001805"
     CDS             2197..4065
                     /locus_tag="ACFM4Z_001805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC6021791.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFQARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLVKDIPNV
                     HWVDAQKYLPKNTVEIHGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSRDGALQ"
BASE COUNT          941 a         1096 c         1067 g         1015 t
ORIGIN      
        1 tttttgacgg tgtacaatcg ctgtttttga acggaggatg gaatggagaa tacaaaccgt
       61 gtgccggagc aggcacgtta tgatgccgaa cgcaggcagg cagacgaagc attggcgggc
      121 gtgtttccgg cagtcagtat cttcggcagc gcgcgcacgc cgcagaatca tgcggattat
      181 gcgttcgcct gccgtctggc gcggcggctg tcggattcgg gcattgccgt catttcgggc
      241 ggcgggccgg ggattatgga ggcggcaaac aagggcgcgt ttgcagggaa gtcggtttcg
      301 gtggggctga acatcgtttt gccgcacgag cagaaaccga atccgtatca ggacatcgcc
      361 ttgcggtttt cccgttttgc cgaacgcaag gcggtgtttt tccgctattc ccaagcatat
      421 gtcgtgatgc cgggcggctt cgggacgctg gacgaattgt ttgaaatcct gaccttggtg
      481 cagacgggca aagtgccgcc gcgtccgatt gttttggtcg gaaaggcgtt ttggtcgggc
      541 ttggcggagt ggataaacgc gcagcttttg gcgcgcggtc tgatttccga aggggcggtc
      601 tctttgtttg ccatatcgga cgatgaagac gaaatcgttg cgtatctgtc ggaacacggg
      661 cttcagacgg catagcgtcc tgagagtgat gtataattgc aaacaattta acaatttttg
      721 atgtctttcc cgaacaggat gccgaaatga tcaaccccat cgcctcgctt tcccctttag
      781 acggccgtta tgcccaatcc gttgaagcat tgcgcccgat tttttccgaa tacggcctga
      841 tgaaagcgcg cgtcaaagtc gaattaaact ggctcaaagc cctcgccgcc gagccgaaga
      901 ttgccgaagt gccgcccttc agtgccgaaa cgcttgccga aatcgacacg gtgattgaaa
      961 acttttcatt ggaagacgcg gccgccgtca aagccatcga agccacgacc aatcatgatg
     1021 tcaaagccat tgaatattgg cttaaagaac gcttctccgg cgtgcctgaa gtggcagctg
     1081 ccagcgagtt catccacttt gcctgcacca gcgaagacat caacaacctg tcccacgctt
     1141 taatgctgca agaagcgcgt gaggctgttt tgctgccgaa actggccgaa atcatcgaaa
     1201 aactgaccgg catggcgcat gagttggctg ccgtcccgat gatgagccgc acccacggcc
     1261 agcctgctac accaagcaca ctgggtaaag aaatcgccaa tgtcgtgtac cgcctgcaac
     1321 gccagtttaa aaacctgcaa gcgcaagagt tcctcggcaa aatcaatggc gcagtcggca
     1381 actacaatgc ccatatggtc gcttatcccg atgtggattg ggaaacccat tgccgcaact
     1441 tcgttgaaat cagcctcggc ctgaccttca acccctacac cattcaaatc gagccgcacg
     1501 actatatggc ggaattcttc caagccatca gccgtgtcaa cacgattctg atcgacttca
     1561 accgcgatgt ttggggctat atttctttgg gttacttcaa acaaaaagtc aaagcaggcg
     1621 aagtcggttc ttccaccatg ccgcacaaag tcaacccgat cgactttgaa aactccgaag
     1681 gcaacctcgg tatggcaaac gccgtattgg gcttcttatc cgaaaaactg ccgatttccc
     1741 gttggcagcg cgacctgacc gacagcaccg tattgcgcaa tatgggcgta ggcgtgggct
     1801 atgccgtgtt gggtttcgcc gcccatctgc gcggtctgaa caagctcgaa cccaaccctg
     1861 ccgcgcttgc cgccgatttg gacgctactt gggagctgct cgccgagccg attcaaaccg
     1921 taatgcgtcg ttacggcgta gccaatcctt atgagaagct gaaagacctg acgcgcggca
     1981 aagacggcat tacgcctgaa gtgttgaaac tctttatcga gtcgttggaa attccggcag
     2041 aagccaaagc caaattgctt gagctgaccc ccgcactgta tgtgggtaag gctgaagcgt
     2101 tggcgaaacg gatttgagcg tttactgaaa ccgatgccgt ctgaacgcgc gttcagacgg
     2161 catttttaag ataacgggac atacggggcg atatttatgc aagctgtccg atacaggcct
     2221 gaaattgacg gattgcgggc cgttgccgtg ctatccgtca ttattttcca cctgaataac
     2281 cgctggctgc ccggagggtt tttgggggtg gacattttct ttgtcatctc gggattcctc
     2341 attaccggca tcattctttc tgaaatacag aacggttctt tttctttccg ggatttttat
     2401 acccgcagga ttaagcggat ttaccctgcc tttattgcgg ccgtgtcgct ggcttcggtg
     2461 attgcctctc aaatcttcct ttacgaagat ttcaaccaaa tgcggaaaac cgtggagctt
     2521 tctgcggttt tcttgtccaa tatttatctg gggtttcagc aggggtattt cgatttgagt
     2581 gccgacgaga accccgtact gcatatctgg tctttggcag tagaggaaca gtattacctc
     2641 ctgtatcctc ttttgctgat attttgctgc aaaaaaacaa aatcgctacg ggtgctgcgt
     2701 aacatcagca tcatcctatt tctgattttg actgccacat cgtttttgcc aagcgggttt
     2761 tataccgata ttctcaacca acccaatact tattaccttt cgacactgag gtttcccgag
     2821 ctgttggcag gttcgctgct ggcggtttac gggcaaacgc aaaacggcag acggcaaaca
     2881 gcaaatggaa aacggcagtt gctttcatca ctctgcttcg gcgcattgct tgcctgcctg
     2941 ttcgtgattg acaaacacaa tccgtttatc ccgggaatga ccctgctcct tccctgcctg
     3001 ctgacggcac tgcttatccg gagtatgcaa tacgggacac ttccgacccg catcctgtcg
     3061 gcaagcccca tcgtatttgt cggcaaaatc tcttattccc tatacctgta ccattggatt
     3121 tttattgctt tcgcccatta cattacaggc gacaaacagc tcggactgcc tgccgtatcg
     3181 gcggttgccg cgttgacggc cggattttcc ctgttgagtt attatttgat tgaacagccg
     3241 cttagaaaac ggaagatgac cttcaaaaag gcatttttct gcctctatct cgccccgtcc
     3301 ctgatacttg tcggttacaa cctgtacgca agggggatat tgaaacagga acacctccgc
     3361 ccgttgcccg gcgcgcccct tgctgcggaa aatcattttc cggaaaccgt cctgaccctc
     3421 ggcgactcgc acgccggaca cctgcggggg tttctggatt atgtcggcag ccgggaaggg
     3481 tggaaagcca aaatcctgtc cctcgattcg gagtgtttgg tttgggtaga tgagaagctg
     3541 gcagacaacc cgttatgtcg aaaataccgg gatgaagttg aaaaagccga agccgttttc
     3601 attgcccaat tctatgattt gaggatgggc ggccagcccg tgccgagatt tgaagcgcaa
     3661 tccttcctaa tacccgggtt ccaagcccga ttcagggaaa ccgtcaaaag gatagccgcc
     3721 gtcaaacccg tctatgtttt tgcaaacaac acatcaatca gccgttcgcc cctgagggag
     3781 gaaaaattga aaagatttgc cgcaaaccaa tatctccgcc ccattcaggc tatgggcgac
     3841 atcggcaaga gcaatcaggc ggtctttgat ttggttaaag atatccccaa tgtgcattgg
     3901 gtggacgcac agaaatacct gcctaaaaac acggtcgaaa tacacggccg ctatctttac
     3961 ggcgaccaag accacctgac ctatttcggt tcttattata tggggcggga atttcacaaa
     4021 cacgaacgcc tgcttaaatc ttctcgcgac ggcgcattgc agtagcctgc cttgccgtcc
     4081 gatatcgttt gtgccgccgt ttgcctttcg gggcggcgg
//
LOCUS       DAXHNE010000154         4098 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.154, whole genome shotgun sequence.
ACCESSION   DAXHNE010000154 DAXHNE010000000
VERSION     DAXHNE010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.154"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(403..1215)
                     /gene="murI"
                     /locus_tag="ACFM4Z_001806"
     CDS             complement(403..1215)
                     /gene="murI"
                     /locus_tag="ACFM4Z_001806"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFC6021792.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
     gene            1405..1866
                     /gene="tsaE"
                     /locus_tag="ACFM4Z_001807"
     CDS             1405..1866
                     /gene="tsaE"
                     /locus_tag="ACFM4Z_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFC6021793.1"
                     /translation="MPDFPPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLEPFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGGGRKCLLTAHTERGRESLPL"
     gene            1863..3113
                     /locus_tag="ACFM4Z_001808"
     CDS             1863..3113
                     /locus_tag="ACFM4Z_001808"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC6021794.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPTHAQVFALPPVGGFKNRLVVDLYPQGMDADDPMMALLNGS
                     PNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMLDPGHGGEDPGAISPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKQG"
     gene            3193..4011
                     /locus_tag="ACFM4Z_001809"
     CDS             3193..4011
                     /locus_tag="ACFM4Z_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6021795.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPDPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FAPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDISRNFSL"
BASE COUNT          995 a         1205 c          999 g          899 t
ORIGIN      
        1 gggaatgacg gtttagaagt tacccgaaac ctcaaaaaaa ccgaaaccga acggtctgga
       61 ttcccgcctg cgcgggaatg acgggtttcg agattacagt gtatcgagaa ggatgggaaa
      121 cggtgggaat tgtgtaaaaa atgccgtctg aagcctttca gacggcattt gcggcattcg
      181 gacatttaaa aatcccatca ttcccgcgca ggagggaatc cgaatacatc tgcacagaaa
      241 cctgcatccc gtcattcccg cgaaagcggg aatctagaac gtaaaatcta aagaaaccgt
      301 tttatccgat aagtttccgc accgacaggt ctagattccc actttcgtgg gaatgacggg
      361 attttaggtt tctgtaatct cgggcagctt tccgtcatcg ttttaaccca aagacaccat
      421 ttcaatctgc tccatcgtcc tgcccagaaa acgctcgccg atggttctga atttcaaagg
      481 aatatcgctg acataaaaac ggtagtcggg attgttgttg tcggtattga gcaatccttc
      541 ctgcgcaagg acgcgtgcgg tttcttcggc agtcgtaatc gcggaatcga ccagcgcgac
      601 attgtgcgcc tccctgccga ttaagggttt gagcaaggga aagtgcgtgc agcccaacac
      661 cagcgtatcg atgccgtctg caagcaatgg tttgaggtat tcgcatacgg tcaggcgggt
      721 aacttcgtgt tccaaccaac cttcctccac caaagggacg agcagcggcg cggcctgcgt
      781 gcggacaagc gtgtcggggt tgtccctatg gatggcgcgc gcataagcat tgctgttaac
      841 tgtggtattg gtggcgataa tgccgatttt gttgttgcgc gtcgttgcca acgcggcttt
      901 cgcgccggcg gaaatcacgt ccaaaaccgg catattgccc gtcttttgac ggattttctg
      961 ccccgccacc gccgcaatcg tattacacgc gataaccatc gccttgacat cgtgttccaa
     1021 taaaaaatcg acaatctgca tcgagaaatt ttcgatggtc gccttagatt tcgtcccgta
     1081 aggcacgcgc gccgtatcgc cgaaataaat gatgttctcc atcggcagcc gctccatcag
     1141 tgcgcgcaca ttggtcaaac cgccgattcc cgagtcaaaa acgccgatgg gtcgctgcct
     1201 gccgatattt tccatccttt ttccaatccg tcctaaacat acaatccgcg tattgtacac
     1261 gcgccgcttt ttcttgacag ccgttgccgt ctgaaagcag gatgcggatt tggctgttat
     1321 aatgtacggc aatgctgccg ccgggcgcac ggtttgccga ccgcctgccc ctccgatccg
     1381 ccacataaaa gttgtaaagc ccctatgcct gattttcccc ccgtttcccg attccttgcc
     1441 gacgaagccg ccacactcga tttgggcgcg gcgtggtctt cccgtttaaa cgcaccgctg
     1501 gtcatttatc tcgaagggga tttgggtgcg ggcaaaacca cgctgacacg cggcatcctg
     1561 cgcggattgg gtcatcaggg cgcagtcaaa agcccgacct acgccatcgt cgaatcttat
     1621 ccgctggaac cctttaccct gcaccatttc gacctctacc gcttctcgtt ccccgaagaa
     1681 tgggaagacg cggggcttga cgaactgttt gccgcaaaca gcgtctgcct gatcgaatgg
     1741 ccgcaacagg gaggcgaatt taccccgccc gccgacatca ccgcaacatt gacacacgac
     1801 ggcggcggca gaaaatgcct gctgaccgcc cataccgaac gaggacgcga aagcctgccg
     1861 ctatgaccaa actgacacga agacaaatca tccgccgcac cgccggcaca ctgttcgccc
     1921 taagccccat cgcatccgcc gttgccaaaa cggtacgcgc cccgcagttt accgccgcac
     1981 ggatatggcc gtcgcacacc tacacccgcc taacgctgga aagcaccgcc gcgctcaaat
     2041 accagcactt cacgctcgac aacccgggca ggctggtcgt cgatatacaa aacgcgaaca
     2101 tcaataccgt attgcacgga ctgtctcaga aggttatggc ggacgacccc tttatccgca
     2161 gcatacgcgc gggtcagaac acaccgacca ccgtccgcct cgtcatcgat ttgaaacagc
     2221 ccacccacgc acaagtcttc gcgcttccgc ccgtcggcgg ctttaaaaac cgcctcgtcg
     2281 tcgatctcta tccgcaaggg atggatgccg acgatccgat gatggcgctg cttaacggca
     2341 gcccgaacaa aacccggcgc ggctctccgg aagccgacct cgctcaaaac actcccgcac
     2401 aaaccgaacg cggcaaaaac gggcgcagac ccgtcatcat gctcgatcca ggacacggcg
     2461 gtgaagaccc cggcgccatc agcccgggcg gtttgcagga aaaacacgtc gtcctctcca
     2521 tcgcccgcga aaccaaaaaa caattggaag ccttgggtta caacgtcttt atgacgcgca
     2581 acgaagacgt gttcatccca ttgggcgtgc gtgtcgccaa agggcgagca cggcgggcgg
     2641 acgtattcgt ctccatccac gccgatgcct tcaccagccc ctccgcgcgc ggcacggggg
     2701 tttatatgtt aaacaccaaa ggcgcaacca gctctgccgc caaattcttg gaacaaaccc
     2761 aaaataatgc cgacgcggtc ggcggcgtac cgaccagcgg caaccgcaat gtcgataccg
     2821 ccctgctcga tatgacccaa accgccacgc tgcgcgacag ccgcaaactc ggcaaactgg
     2881 tgcttgaaga attgggcagg ctcaaccact tgcacaaagg cagggtggac gaagccaatt
     2941 tcgccgtttt gcgcgcaccc gatatgccgt ctatcctggt cgaaaccgcc ttcctgtcca
     3001 accctgccga agagaagctg ctgggcagcg aatccttccg ccgacagtgc gcccaatcca
     3061 ttgcctcggg tgtccaacgc tacatcaaca cttccgtatt gaagcagggt tgattgaaaa
     3121 ggcggtgttt caatatagtt gtcggatgaa ggcaggcata aacctacttc cgccccaaac
     3181 cgcgttttta tgatgaaagg tatggataaa ttgagatacc aacaggattt tttaaatatc
     3241 cgccccattt ttacagccgg cgaacaagaa tacctgaccg aattgtccga tcggcttcct
     3301 ttatctgttt taacggactc cgtgcgtaat attgaagaaa tcggtatcga ttttgtgtac
     3361 agttctgcca aattggaagg aaacacttat aatcaatacg atacgcaggc attgcttaaa
     3421 ctcgggcaaa ccgcaggcgg gaagttatat tcggatgccg taatgttaat caacctgagg
     3481 gaaagttacc ggcacctgct gtccggtttg gacagccccg atccgtttga ctggttggat
     3541 ttcttaaaaa ccacccacag cctgatttcg gaaaacttac tggaaaaagg ctccggcggc
     3601 gtagtccgcc gcgacagcgt tactataagc gggacagact acacgccttt atccaacccg
     3661 caaagtttgg atacggaatt aaaatggttg cttcaagaag cccccaaaat agaaaatcct
     3721 ttcgaccgcg ccgtttatct ccataataat ttggcgtatc ttcaatattt taaagattgc
     3781 aataaacgta ctgccagaaa ctgcatgacc ctatcgctga tgcgctccgg tttttttcct
     3841 tgcgtattcg cccccgacag ttatcccgcc tatgccgagg ctgtcgtagc ctattacgaa
     3901 accggcgatt acggtttatt caagaaatat tttatttcgg catacgaaaa taccgtaaat
     3961 aagtacggcc cacagcctga tatggatatt tcccgcaact tctcccttta attccaaggg
     4021 gttgttatcc gtatcttccg cacgcggcaa atgcggcggc acagtggaca cacccctccc
     4081 ttaccttcgg ttatagtg
//
LOCUS       DAXHNE010000155         4047 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.155, whole genome shotgun sequence.
ACCESSION   DAXHNE010000155 DAXHNE010000000
VERSION     DAXHNE010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.155"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(99..1460)
                     /gene="accC"
                     /locus_tag="ACFM4Z_001810"
     CDS             complement(99..1460)
                     /gene="accC"
                     /locus_tag="ACFM4Z_001810"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFC6021796.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAIYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(1574..2026)
                     /gene="accB"
                     /locus_tag="ACFM4Z_001811"
     CDS             complement(1574..2026)
                     /gene="accB"
                     /locus_tag="ACFM4Z_001811"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFC6021797.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQQ
                     VKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(2008..2265)
                     /locus_tag="ACFM4Z_001812"
     CDS             complement(2008..2265)
                     /locus_tag="ACFM4Z_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HFC6021798.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            2382..3422
                     /gene="queA"
                     /locus_tag="ACFM4Z_001813"
     CDS             2382..3422
                     /gene="queA"
                     /locus_tag="ACFM4Z_001813"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFC6021799.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLHRLKDKGAVTAEVTLHVGAGTFQPVRVEKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVADRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
BASE COUNT          870 a          983 c         1210 g          984 t
ORIGIN      
        1 ttaatccact atatcgaaat cggtttcaga cggcgcgggg caagggaaat gccgtctgaa
       61 cgcggacggg cggcttcaga cggcatatcg gcggcggttt acttgtcctg tttggctttg
      121 cgatcttcca gccagtgttc caaatagtgg atgctgacgc cgcctttttg gaaacccgca
      181 tcggcgaaca ggtcgcggtg aagcggcgta ttggttttga tgccggttac cgccagctcg
      241 gcgagtgcga cgcgcatttt cgccattgcc tgttcacgcg ttttgccgtg tacgcagatt
      301 ttgccgatca ggctgtcgta gtacggtggg atgcggtagc cttggtaaat gtggctgtcc
      361 acgcggatac cgaagccgcc gggcaggtgg cagctttcaa tcgggcccgg gctggggatg
      421 aagttgtacg ggtcttcggc attgatgcgg cactcaaacg cgtggccttc gacttgaata
      481 tccttttgtt tgtattgcaa aggcaggccg gcggcgatgc ggagttgctc ctgcacgatg
      541 tccacgccgg taatcagctc ggtaaccgga tgctcaacct gaacgcgcgt attcatctcg
      601 ataaagaaaa attcgccatc ttcgtacagg aattcaaacg tacctgcgcc acgatagccg
      661 atgcgtttgc aggcgtcggc acaggcgttg ccgattttgg cgcgttcttt ttcagtgatg
      721 aacggagccg gtgcttcctc gatgactttt tggtggcggc gttgcagcga acagtcgcgc
      781 tcggcaaggt agatggcgtt gccgtgttcg tcggcaatca cttggatttc gacgtggcgc
      841 ggacgttgca gatagcgttc catgtaaacc atcgggttgc cgaatgccgc gcctgcttct
      901 gccttggtca tttcgacaga ttggaggagg tcttcttttt tctcgaccac gcgcataccg
      961 cgaccgccgc cgccgccaga ggctttgata atgacgggat aaccgacttt atcggcgatt
     1021 ttgaggattt cttcgccgtc gtcgggcaat gcgccgtcag aaccggggac gcagggtacg
     1081 cctgccgcta tcatcgcgtg tttggcggag actttgtcgc ccatcaggcg gatggtgtcg
     1141 ggtttcgggc cgataaaggt aaagccggac tgctcgacct gttcggcgaa atcggcgttt
     1201 tcggcaagga aaccgtagcc cggatggaca gcgtccgcgc agcttacttc ggcggcggcg
     1261 ataatggcgg ggacgttaag gtagctttgc gcggaagcgg cagggccgat gcacacggat
     1321 tcgtcggcga gtttgacgtg caggctgtct ttgtcggcct cggaatgcac ggcgacggtg
     1381 gcaatgccca tttcgcggca ggcacggagt acgcgtaatg cgatttcgcc tcggttggcg
     1441 attaaaactt ttttcagcat gatgactgtt cctgtggttc cggcaacccg tccgtaaaaa
     1501 aaaaggtgcg ggaacgccga agggggaaag catttcagac ggcatcggaa gtttatgccg
     1561 tctgaaaaca ggattatccg atgatgaaga gcggttcgcc gaattcgacg ggcgtaccgt
     1621 tttcgaccag aatttctttg accgtgccgg atttttcggc ttcgatttcg ttcatcagct
     1681 tcatcgcttc gatgatgcac agcgtgtcgc cggctttaac ctgttggccg acttcgacaa
     1741 aaggcgcggc attcgggccg ggtgcgcggt agaacgtgcc gaccataggc gatttttgcg
     1801 cgtcggacaa atcgcgggcg gcaggtgcgg cgggcgcgga tgccgcaacg ggtgcggcgg
     1861 caggcgttac ggccggcgcg gcggcaggta cgggcgcggc gtaaacgggt gcggcggcga
     1921 tggttcgggt gatgcggact ttttcctcgc cttcggttac ttcgatttcg gcgatacccg
     1981 attcttcaac caaatcaatc agttttttta atttgcgcaa atccatttct gttcctttaa
     2041 aggctgccgg tgttccggcg ggttgttgcg ttggtttttc agaaaacagc cggcttgcgg
     2101 caacggcgca aaccgggcgg aatggtgttc ggatacggtt ttcctgtggt ggaaaacaac
     2161 cgtgagaatg tgtttatttt cccgaagttg tcggtaaatg tccagtaaaa tatcaaaaaa
     2221 cggcggtttt tgggggaaat gtgcaggaag cttgattttg cgcacaagat gccccgggtc
     2281 aagagtgttt attctaatct gttggttttt cggacaaaga tgccgtctga aaaagggcta
     2341 aagtgcgtat aatggcggct tgcccgaacg agagtgtaaa aatggatatt tcagattttg
     2401 attttaccct gcccgaaaag ctgattgccc agcatccgcc cgaagtgcgc ggcagcagcc
     2461 gccttttggt tgcgttgccc gatatgccgc tgcaagaccg ggtgttcggc gatttgccgg
     2521 attatgtcga ggcgggcgac gttttggtgt tcaacaacac caaagtcatg aaggcgcggc
     2581 tgtttgggca gaaagacagc ggcggcagga tcgaagccct gattgagcgc gtgttggaca
     2641 accataccgc actggcgcac atccgttcgt ccaagtcccc caagcccggt atggggctgg
     2701 tgtttgaagg cggcatccgt gccgtgatgg tcgggcgtga gggcgaactg ttctgcctgc
     2761 gttttgaagg cggtcaaacc gtttacgaac ttttggaaca gaacggccat ttgcctctgc
     2821 cgccttatat cgaacgtgcc gccgacgcgg acgacgacag ccgttatcaa accgtttatg
     2881 ccaaatatca gggcgcggtc gccgcgccga cggcgggcct gcattttacg gaagaacttt
     2941 tgcaccgtct gaaagacaaa ggcgcggtaa ccgcagaagt aactctgcac gtcggtgcgg
     3001 ggacattcca acccgtgcgt gtggaaaaaa tcgaagaaca caaaatgcac agcgaatggt
     3061 ttgaagtgcc gtctgaaacc gccgccgccg ttgaggcggc aaaagcccgg gggaacaaag
     3121 tctgggcggt cggcacgact tccatgcgcg ccctcgaatc tgccgcgcgt gcgacgggtc
     3181 gtctgaaagc ggggcagggc gacaccgata ttttcatcac gccgggctac cgttttaatg
     3241 ttgccgacag gctggttacc aatttccatc tgccgaaatc gacgctgctg atgcttgtca
     3301 gcgcgttttc gggtatgggt catatccgcg ccgcgtatcg ttatgcggtc gaacgcgaat
     3361 accgtttctt cagctacggc gatgcgatgg ttttggggcg gaacgaaggg gtggtacgtt
     3421 agaacagcct caagtctatt gggggaagac aagtgccgtc tgaacggatg ttcggacggc
     3481 attcttttgt tgatttgaat gaaaggaact gcatgacata tagtggatta aatttaaatc
     3541 aggacaaggc aacgaagccg tagacagtac aaatagtacg gcaaggcgag gcaacgctgt
     3601 actggttttt gttaatccac tatagattca ggcgcgagag gcgagaaact cagtcattgt
     3661 tgatgacgcg caatacgtta aaattcattc cgttttggtc gtataaacat attacatacg
     3721 ccattttttt gtattcaagg ctgcctgaaa acagtgggca agaatatcta acccaacgaa
     3781 aagctgaaaa agcaacaaaa aataatctgc accttagtcg gttggttagt ccaaccttag
     3841 gggtgcagac cattcaatct ttcaggctgc ctttgtttgt ttataagata aatttttagc
     3901 cgaacacttt ttccaagtgt gcctgataat ctgccaagta tttttccact tgtggatttt
     3961 taaccacatc gttacataag aatgtcggca ggcgggtcat acccaaaaac tcgttggctt
     4021 tgtggaagtg catatacaaa acatcaa
//
LOCUS       DAXHNE010000156         4017 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.156, whole genome shotgun sequence.
ACCESSION   DAXHNE010000156 DAXHNE010000000
VERSION     DAXHNE010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.156"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            120..1025
                     /gene="glyQ"
                     /locus_tag="ACFM4Z_001814"
     CDS             120..1025
                     /gene="glyQ"
                     /locus_tag="ACFM4Z_001814"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFC6021800.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            1101..1511
                     /locus_tag="ACFM4Z_001815"
     CDS             1101..1511
                     /locus_tag="ACFM4Z_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding protein YggL"
                     /protein_id="HFC6021801.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQALIFGVRGSLHQAA
                     DDAEAFDRFMDELIAYLEANGLEMAGGGDCGDFSCLFQKSGARSLGETEKKAIISWLA
                     GREDVATLYATPLVDGFYGDDGWENYSETYKQAD"
     gene            1532..3595
                     /gene="glyS"
                     /locus_tag="ACFM4Z_001816"
     CDS             1532..3595
                     /gene="glyS"
                     /locus_tag="ACFM4Z_001816"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFC6021802.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGGLLEDIINQVVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAEQMRGQGKVVA
                     SFAERKAAIQTALEGQARSLNAAVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLSSVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAVEQHYQPRFAGDKLPESKIA
                     TAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGEVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3612..4013
                     /locus_tag="ACFM4Z_001817"
     CDS             3612..4013
                     /locus_tag="ACFM4Z_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6021803.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLATVVNQ
                     TWRNLEILIVDDGSTDGTLAIAKDFQKRDSRIKIRAHDRLLNRKQQRRDSPANTFKHR
                     LIRALTKISREREKRRQRREQLIGKIIVPFQ"
BASE COUNT         1029 a         1170 c         1027 g          791 t
ORIGIN      
        1 aatttaatcc actatatcat gtatgccgtg ttttcaaatc agtctgtctt tgagataaac
       61 ctatccaaac agcaatggaa acagtaaaat aaacccttta ttttatcgaa tcataagcca
      121 tgcttacctt ccaacaaatc atcttcaaac tgcaaacatt ctgggcagac aaaggctgca
      181 ccgtcatcca acctttcgac atggaagtcg gcgcaggtac ttcccacccc gccacctgcc
      241 tgcgcgcact cggccccgag ccttggtttg ccgcctacgt ccaacccagc cgccgcccca
      301 aagacggccg ctacggcgac aaccccaacc gcctgcaaca ctattaccaa ttccaagtcg
      361 cccttaaacc cgcccccgcc aatatccaag acctctatct cgactccctg cgcgaattgg
      421 gcatcgaccc caaagtccac gacatccgct ttgtcgaaga cgactgggaa aaccccaccc
      481 tcggtgcatg gggtttgggc tgggaagtct ggctcaacgg catggaagta acccagttta
      541 cctatttcca acaagtcggc ggcattgact gcacgcctgt actcggcgaa attacctacg
      601 gcatcgaacg cttggcgatg tacctgcaag gcgtggaaaa cgtctatgac ctcgtttggg
      661 caaaaacgcc ggacggcaac accgtaagct acggcgacgt gtaccaccaa aacgaagtcg
      721 aacaatccac ctacaacttc gaatacagcg atgccgattg gctgctgcgc cagttcaacg
      781 actacgaagc gcaagccaaa cgcctgcttg ccgaagaaaa cgccgccctc gccctgcctg
      841 cctacgagct ggtcctcaaa gcggggcata cgttcaacct cttggacgcg cgcggcgcga
      901 tttccgtaac cgaacgcgcc acctacatcg ggcgcatccg cgcgttgagc cgcgccgtgg
      961 cgcaaaaata tgtcgaaagc cgcgaaaaac tgggctttcc cttaatgaag gcaaatgccg
     1021 cctaggggag cgacgatttt tacgttcggg cggcacgata tggcagccgt ccggaagcca
     1081 ttatattttt taggaaaatt atgcctgtct atttccgcac cccgagcagg aaacgcctgc
     1141 acaaaatgaa ccgccgccag cataaaaagc tgcatttggg cgagtttcaa gcattgattt
     1201 tcggcgttcg aggcagtttg caccaagctg cggacgatgc tgaagcgttc gaccgcttta
     1261 tggacgagct gattgcgtat cttgaggcaa atggcttgga aatggcgggc gggggcgatt
     1321 gcggcgattt ttcgtgcctg tttcaaaaat ccggtgcccg aagcttgggc gaaaccgaga
     1381 aaaaagccat tatctcttgg ctggcaggcc gcgaagatgt cgccactttg tacgccaccc
     1441 cgctggttga tggtttttac ggggatgacg gctgggaaaa ttacagcgaa acctataaac
     1501 aggcagatta accaaattca aagaaagccg aatgatgacc caaacccttt taatcgaact
     1561 ccttaccgaa gaactcccgc ccaaagccct gaataatctg ggcaaccatt ttgccgccgc
     1621 cgttgccgaa ggcttggaaa aagcgcaact ggttgacggt gcagccgaat ttacggctta
     1681 tgcctcgccg cgccgtttgg cggttcaagt caaaaacgtc aaagccgttc aggccgacca
     1741 aaaaatcgtg aaaaaaggcc ctgccgtggc gaatgccgta aaagacggtg cgccgaccaa
     1801 ggctttggaa ggtttcgcac gcggtgcagg cgcgaaaatc gaagacttga ccatcgtcca
     1861 cgacggcaag caggatgtgt acgcctacga atacgtccaa accggcaagc cgctgggcgg
     1921 acttttggaa gacattatca atcaagtggt taagaaactg ccgattccca aagtgatgcg
     1981 ttggggcagc agcacattta ccttcgtgcg ccctgttcac gggctgattg tgctgcacgg
     2041 cggcgacatc gtaaacgtca gcgttttggg tctgcaaagc agcaatcaaa ccttgggaca
     2101 ccgcttcttg tccaacggcg aaatcaccat tgaaaacgcc gacagctacg ccgaacaaat
     2161 gcgcgggcaa ggcaaagtcg tcgcttcgtt tgccgagcgc aaagccgcga ttcagacggc
     2221 attggaaggg caggcgcgca gtctgaacgc cgccgtcgcc gccgatgaag cattgttgga
     2281 cgaagtaacc gcgctggtcg aatggcctgt tgttttggaa gccggttttg aggaacattt
     2341 cctcgccgtg ccgcaggaat gcttgatttt aacgatgcag caaaaccaaa aatactttcc
     2401 gctgctcgac caaaacggca agctgatgaa ccgcttcctg ctggtttcca acctgcaaac
     2461 cgaagacccg tcgcacatca tccgaggcaa cgaacgcgtt ttgcgcgcgc gcctgtctga
     2521 tgccgagttc ttctacaaac aagaccaaaa agcgactttg gaaagccgcc tgccgaaatt
     2581 gtccagcgtg gtgtaccaca acaaaatcgg ctcgcaagcc gaacgcatcg aacgcctgca
     2641 aagcattgcc gcccacatcg ccaaagcatt gggcgcggat gccgccgctg ccgaacgcgc
     2701 cgcgcgtctt gccaaagccg acttggtaac cgaaatggtc ggcgagtttc ccgaactgca
     2761 aggcacgatg ggcaaatact atgcccgctt ggacggcgaa accgaagaaa tcgccgaagc
     2821 cgtcgagcaa cattaccaac cgcgttttgc cggcgacaag ctgcccgaaa gcaaaattgc
     2881 cactgccgtt gcacttgccg acaagctgga aaccctggtc ggcatttggg gcatcggtct
     2941 gattccgacc ggcgacaaag acccctacgc cctgcgccgc gctgccttgg gtattttgcg
     3001 tatgctgatg cagtatggtt tggacgtgaa cgaactgatt cagacggcat tcgacagctt
     3061 ccccaaaggt ttgctcaacg aaaaaacgcc gtctgaaacc gccgacttta tgcaggcgcg
     3121 tttggccgtg ttgctgcaaa acgattatcc gcaagacatc gttgccgccg tattggcgaa
     3181 acagccgcgc cgtttggacg atttgaccgc caaactgcaa gccgttgccg cgttcaaaca
     3241 gctgcccgaa gccgccgcgc tcgccgccgc caacaaacgc gtgcaaaacc tgctgaaaaa
     3301 agccgatgcc gagttgggcg aagtcaatga aagcctgctg caacaggacg aagaaaaagc
     3361 cctctttgcc gccgcgcaag gcttgcagcc gaaaatcgcc gccgccgtcg ccgaaggcaa
     3421 tttccaaacc gccttgtccg aactggcttc cgtcaaaccg caagtcgatg ccttctttga
     3481 cggcgtgatg gtaatggcgg aagatgccgc cgtaaaacaa aaccgcctga acctgctgaa
     3541 ccgcttggca gagcaaatga acgcggtagc cgacatcgcg cttttgggcg agtaaccgtt
     3601 gtacagtcca aatgccgtct gaagccttca gacggcatcg tgcctatcgg gagaataaat
     3661 tgcagccttt agtcagcgta ttgatttgcg cctacaacgt agaaaaatat ttcgcccaat
     3721 cattagccac cgtcgtgaat cagacttggc gcaacttgga gattttgatt gtcgatgacg
     3781 gctcgacaga cggtacgctt gccattgcca aggattttca aaagcgggac agccgtatca
     3841 aaatccgtgc acacgaccgc ctcctgaacc gcaaacagca aaggcgcgat tcccccgcca
     3901 acacattcaa acaccgcctg atccgcgcct tgaccaaaat cagcagggaa agggaaaaac
     3961 gtcggcaaag gcgcgaacag ttaatcggca agatcattgt gcctttccaa taaaagg
//
LOCUS       DAXHNE010000157         4016 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.157, whole genome shotgun sequence.
ACCESSION   DAXHNE010000157 DAXHNE010000000
VERSION     DAXHNE010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.157"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..783)
                     /gene="mafA"
                     /locus_tag="ACFM4Z_001818"
     CDS             complement(<1..783)
                     /gene="mafA"
                     /locus_tag="ACFM4Z_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC6021804.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATQYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTKNSGRKGERSAGLSVNGTGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLITPKTAAYESQYQEQYALWTGPYKV"
     gene            complement(903..2003)
                     /locus_tag="ACFM4Z_001819"
     CDS             complement(903..2003)
                     /locus_tag="ACFM4Z_001819"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFC6021805.1"
                     /translation="METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGADELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAERTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDNRE
                     TRAVLNIGGIANISVLPPDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFARPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAADTRQMYICGGGIRNPVLMADLAECFGTRVSLHSTAELNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            2328..3902
                     /locus_tag="ACFM4Z_001820"
     CDS             2328..3902
                     /locus_tag="ACFM4Z_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HFC6021806.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
BASE COUNT          787 a          968 c         1257 g         1004 t
ORIGIN      
        1 gactttgtaa gggccggtcc aaagggcgta ttgttcttgg tattgggatt cgtaggcggc
       61 ggttttaggg gtaatcagca gtttccggct gtcgcggtca acggcgaaat attcgagctt
      121 ggtttgggct ttaagggttt cggcgttgta gaggtgcagt tcggtacggc tgcggacggt
      181 gccgaatacg tcgacggtta cgaatacgtc ggtgtcggcg tattcgggcg gtacgacttc
      241 gatgccgcgc aggtagaaga cggtttggat gaggttggtc aggaaggaaa cgtcgcgggg
      301 gttggcgagc agggtttcgt tgcggtagtc gcccgtgccg ttgacggaca gtccggcgga
      361 gcgttcgcct ttgcgtccgc tgtttttcgt cagggcggcg gcgggggcgt tcaaaagcga
      421 tgtggaagtg gttacgctgg agagcgcgtc ggatttggtg gtggcggtag tgtcgtaggc
      481 ggggtagctg tattgggtgg cactttcggg gttgttgtgg tagccgccgc gtatcagtgc
      541 gtcgatagag tagcgtccgc cgcttatgtt gcccgaacct tggtcgccca taacggagac
      601 gtaaagggcg gctttgcgtc cttttagggc ggacaaatcc atttctttga cggcggcgcg
      661 ggacgatgcg gcgacgagtt cttgttcgac ggcaaagcgt ttgccgccgc cgtgggcggg
      721 tatgccggtc agtgtgccgc aggctgtgag gacgaggggg atgaggagga gcagggtttt
      781 catagcgggg tttgtttgat gttgaacgga ttttgagtgt aaagggattt taagggtttg
      841 taaacaaaag gggcgaaaat gccgtctgag cggcggaaat ggctttcaga cggtatttgc
      901 gctcaataat aatatcccgc gcccagaata cacggtttgg atgcgccggt tgctttgtgc
      961 gggctgccgg gaatgcggtt aatccaacac gccgccaacc acgcaaatgc ggctgcctcc
     1021 acccattgag gatcgaggtt cagttcggcg gtgctgtgca gggaaacgcg tgtgccgaaa
     1081 cattctgcca aatccgccat taaaacagga ttgcggatgc cgccgccgca aatatacatt
     1141 tgacgggtat ctgccgctgc gtgtgagacg gcgtcgaaaa cggtttgcgc ggtaaaacgg
     1201 gaaagcgtcc gcaatacgtc gtatcggttt tcgccgccgt caaggtaggt ttcgagccaa
     1261 tttagggcaa acagttcgcg ccccgtgctt ttggggtggg gtcgtgcgaa atacgggtgg
     1321 gcgagcagcc tgtcgagcag ttgcggcaat atgttgcctt gtgccgcctt tgcaccgttt
     1381 ttgtcgtaag gaagctgcca gtgtgcctgc gtccacgcgt ccatcagcat attgcccggc
     1441 cctgtgtcga agccgaaggc gggtgcgtcg ggggggagta cgctgatgtt ggcaatcccg
     1501 ccgatgttca gtaccgcgcg tgtttccctg ttgtcgcgga acagggcttc gtgaaaggcg
     1561 gggacgagtg gcgcgccttg tccgccggcc gcaaggtcgc ggctgcggaa gtcgccgacg
     1621 gtaaaaatcc gcgtccgttc cgccagcagc ggcaaatcgg caagctgtat gctgtaaccg
     1681 tgttccggcg cgtgtcggac ggtttgcccg tggcagccga gggcggtaat gtcggacggt
     1741 gcgaggtttt gactgcacag cagttcggcg gcggtttgcg cgtacaggcg gctgagttct
     1801 tgcgacaaaa tcctgctgcg gtgcagttcg tctgcgcctg tgtcctgcaa atccagcaat
     1861 tggcggcgca gcctgtcggg atagggggta aaggcgtgcc cttccgcgcc cagccatttg
     1921 ccgccgtcca tccgtatcag tacggcatcc gccccgtcca tgctggttcc cgacatgatg
     1981 ccgatgtaaa gctgtgtttc catcatcact cccaaactgg tgcaaaacgc cattttaacg
     2041 tgtattgacg ctcgtatacc gatttgccgc cgcagtgtaa ataaagtgta aataaatgtt
     2101 tcaagacgga tggaaaaata ttataatgcg cccgcaacat ccagtagtag aagtgtcata
     2161 caaaccgttt ccggcagcag ttttgcattc ggtcaggttt gggggtattc ggatgcggtt
     2221 cagaaggaag gatgcgtctg ccatatcccg aaacggcagt tcgaccggag gcagcagtac
     2281 agtgtcggca acactcatga tttccaccac attaaaggaa gattgccatg gctcaaatcc
     2341 aaatgagcgc aaatgttaaa accatcaacg ccgtctttgc cgccatgctg gtaggtacag
     2401 tcggctattt tatttattgg ggcttgggtt atacccatta caattacgcc gccttattca
     2461 ttattgccac gatgttcggc gtgtttatgg cgttcaacat cggcggcaac gatgttgcca
     2521 attctttcgg caccagcgtc ggtgcgggta cgctgaccat cccgcaggct ttgctgattg
     2581 cggcggtatt tgaggtcagc ggcgcggtca tcgcgggcgg cgaggtaacc aataccatac
     2641 gcaaaggcat cgtcgatttg aagggtgttg atttcgaacc catacagttt gtgtttatta
     2701 tgatgtccgc gcttttggcg gcggcgttgt ggctgttgtt tgcctcgaaa aaagggcttc
     2761 cggtatctac cacccattcc attatcggcg gcattgtcgg cagcgcggta tgtatggcgg
     2821 taatgaacga tgccgcatcg ggcgatttga tacgttgggg caagctgggc ggtattggtg
     2881 tttcttgggt attgtcgccc gtgttgggcg gcgcggtgtc ctatttcctg ttttcgcgcg
     2941 tcaagaaaaa cgtcttagat tacaacgctt gggcggaagg cacgctcaag ggcatcaagc
     3001 aggaaaaaaa ggcctataaa gaacggcacc gcctgttttt cgagggtttg tccgaagccg
     3061 aaaaagtcga gtacgccacc aaaatggcgc acgacgcgca aatttacgac gaacccgaat
     3121 tcgatccgca agagctgcaa tcggagtatt accgcggtct ttatgcgttc gacaaccgta
     3181 aaaacaatgt cgattcctac aaagcgctgc attcttggat tccctttatc gcttcgttcg
     3241 gcgcgatgat gatttccgct atgctgattt tcaagggctt gaaaaacctg catttgggga
     3301 tgagcaacgt caacagcttc ctgaccatct ttatgatagg cgcggcggtg tggatgggga
     3361 cgtttgtttt tgccaaaagc ctcaagcgta aagacttggg caaatcgacc tttcagatgt
     3421 tttcatggat gcaggtcttt accgcctgcg gcttcgcatt cagccacggt gcgaacgata
     3481 tcgccaacgc catcggtccg tttgccgcga ttatggatgt tttgcgtacc aacagcgttg
     3541 ccgcgcaaaa tgtcgtcccc ccgattgcga tgctgacttt cggcatcgcg ctgattgtcg
     3601 gtttgtggtt tgtcggtaaa gaggtgatta aaaccgtcgg tacgagtttg gccgaaatgc
     3661 accccgcctc gggttttacc gccgaactgt ccgccgcatc cgtcgtgatg ggcgcgtcgc
     3721 tgatggggct gcccgtgtcc agtacgcata tcttggtcgg cgcggtactc ggcatcggtc
     3781 tggtcaaccg taatgccaat tggaaactga tgaagcccat cggtttggcg tgggtcatta
     3841 ccctgcctgc cgccgccgta ttgtcggttg tctgctactt ggttttacag gcagtattct
     3901 gattgtaaaa tactgatgcc gtctgaaccc gtgttcagac ggcattttgt tgatggaatg
     3961 tgcgggcttg tgccttatgc gcaatctgtt ctgtcgggat atgccgtttg gtatag
//
LOCUS       DAXHNE010000158         4007 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.158, whole genome shotgun sequence.
ACCESSION   DAXHNE010000158 DAXHNE010000000
VERSION     DAXHNE010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.158"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(76..270)
                     /locus_tag="ACFM4Z_001821"
     CDS             complement(76..270)
                     /locus_tag="ACFM4Z_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFC6021807.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(260..2341)
                     /locus_tag="ACFM4Z_001822"
     CDS             complement(260..2341)
                     /locus_tag="ACFM4Z_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFC6021808.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPDRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPTLQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVVLWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            2818..3198
                     /locus_tag="ACFM4Z_001823"
     CDS             2818..3198
                     /locus_tag="ACFM4Z_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HFC6021809.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMPPVQNQAGTDDF
                     RAFSCENGLSVRVRHLDSGKVALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3195..3476)
                     /locus_tag="ACFM4Z_001824"
     CDS             complement(3195..3476)
                     /locus_tag="ACFM4Z_001824"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021810.1"
                     /translation="MTKGRLKNSFDKDAVAKEIDLQREVTQAFGKTPPKPQRPLPTNS
                     AIPEVTDGIRQPEPCRRPNCKTNPTQKTVRPTQKTARKIWTAVQTGCPV"
     gene            complement(3480..3973)
                     /locus_tag="ACFM4Z_001825"
                     /pseudo
     CDS             complement(3480..3973)
                     /locus_tag="ACFM4Z_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
BASE COUNT          933 a         1011 c         1130 g          933 t
ORIGIN      
        1 tgatttttgt taatccacta tattataaaa attccgtctg aagcggcgtt cagacggaat
       61 ttgtttcaag caggcttaac agcagcgtcc gccgtttgcg ccgcagcggc gtttgcggca
      121 atagtcttga aactgcagct tggtcatcac aggggcgttg ggattatgtt tgcgctgctg
      181 tgcaacgtag ttttcataat cgggcacgcc tgccatcaag tttgccgtca gcttgatggt
      241 tttccaccaa gacgcgagct tatgcttcac tttgtgcctc cggctgtttg ccgtcgcggt
      301 acaccgccgg gatttctttg gcggtcggcc agccgacttt gcgtgctttg agggcggtac
      361 gcaaaccgta cgcggcgaca atcacgacaa ccgacaagaa gaggatggtc agaccggcat
      421 taatcttgtc gttgaagatg atttgcgcca tttcgccgat gtctttggca ggcgcaagga
      481 cttcgttttt agccaatgcg tcgctgtatt tgccggcgtg ggcaaggaag ctgatgcgcg
      541 ggtcgctgtg gaacagtttt tgcaggccgg cgtagcaggt tacgaacagt acgccgacgg
      601 cgggaacgag taccacccag acataacggt cgcgtttcat cttaatcagc accacggcgc
      661 acataatcaa ggccacgcct gccagcattt ggttggcgat gccgaacaaa ggccagagcg
      721 agttgatgcc gcccagcggg tcggttacgc cggtgtagag gaagtagccc cacaatacca
      781 cggcgaagaa ggtcgcaatc aggttggcgg ggatggagtc ggtgttgccg aaaggtttgt
      841 agaagatgct gcccaagtct tgaatcataa aacgtgcgac gcgcgtaccg gcatcgacgg
      901 cggtcaggat gaacaaggct tcaaacaaca gggcgaagtg ataccagaac gccatcatcg
      961 cctcgcccgg aatcaggcgg ctcataatgt gcgccatacc gactgcgagg gtgggcgcac
     1021 cgcctgcacg ggaaaggatg gtgttttcgc ccacttcctt agcggtgtgc aacagggttg
     1081 ccgcatcgac agggaattgc agcttggtgg taatcacttc ggcggcggta ttggcatccg
     1141 taccgatcag ggcggcaggg ctgttcatgg cgaagtacac gccgggatca agcgatgcgg
     1201 cggcggcaag cgccataatg gctacgaaac tttccatcaa cataccgccg taaccaatca
     1261 tacggacgtg ggtttcgttt tccagcattt tcggcgtggt gccggaggaa atcagcgcgt
     1321 ggaagcccga aaccgcaccg caggcgatgg tgatgaagag gaacgggaac aatgcgcctg
     1381 agaataccgg acccgaaccg tcgataaagt gggttacggc aggcatttgc aaagtgggat
     1441 tgacgatgac gatgcccaaa gccaaggccg caatcgtacc gattttcagg aaggtggaga
     1501 gatagtcgcg cggagtgagc agcaaccata cgggcaatac ggaggcgaca aagccgtaaa
     1561 tcataatcgc ccaagtgagc tggatgccgt caaggtcgaa ccaatgtccg atggaacttt
     1621 tcgccacatc ttcgccgtaa atcacggcca acatcagcaa aataaagccg acgatggaaa
     1681 tctcgccaat tttgcccgga cggatataac gcgtgtagat acccataaac agcgcaatcg
     1741 gcatcgttgc ggcaatggtg aacgtacccc aagggctgtg aaccaatgct tttacgacaa
     1801 tcaacgccaa caccgccata atgatgacca taatcatcaa aataccgatg gaggcaatca
     1861 cgccggggac agtgccgagt tcctgtttca caatatcgcc caaagactta ccgtcgcggc
     1921 gcatagagac gaacaagacc atcatatcct gtaccgcgcc ggcaaatacc acgccgaaga
     1981 taatccacaa agtaccgggc agataaccca tttgcgccgc caaaaccgga ccaaccaaag
     2041 ggcccgcgcc ggcaattgcg gcaaagtggt gtccgaacaa tacgcctttg tgcgtcggaa
     2101 cgtagtccaa gccgtcgttg tggcgttctg ccggagtcag gcggtcagga tcgagccgca
     2161 ttacgcggtt ggcgatgtag aggctgtaaa aacggtaggc gatgcagtaa acggatatgg
     2221 cggcggtgac catccatacc gcgctgacct gttcgcctcg gctgagggcc agagtggtaa
     2281 aggatgctaa gccgaccagt accactatgc cccaaatgag gaaggttttg agtgatttca
     2341 tcgaataaat ccttatctca cactgtcgga atatgcctga acggcgggaa acggccgaac
     2401 catctgccgg cacgggcaca aaccggagtc tgaatgattg acgaagtatg aaacagtgcg
     2461 cttgtcggga tttgtgcctt atgcccgcat caaacagtgc agggatgcgg gcgcacaaaa
     2521 cgttaaacgc cgaataggat tttaacgcaa attagcacac cgatagcggt ttttacttgg
     2581 aatttggaaa aaatttacat tcctccgggc gggcaggcag gttcagacgg catcgtcagg
     2641 caaaaggcgg catcggaaga ggggtaaaga aggggcgcgc aatccggatt attgattcat
     2701 cgcaggtaaa ttccggttat cgggcttgtg tgtttgcgcg tccgtttata gtatggcgtt
     2761 gccgcagctt ggaatcaggg cggttgtttc atatcttatt tttattgggg agcttttatg
     2821 aatatcaggt ttttcgcgct gaccgtaccg gttttgtctt tggcggcctg tgccgtgccg
     2881 gaggcgtatg atgacggcgg acgcgggcat atgccgcccg ttcaaaacca agccggcacg
     2941 gacgattttc gggcgttttc ctgcgagaac ggtttgtctg tgcgcgtccg ccatttggac
     3001 agcggcaaag tcgcgttgcg gctggacggc aggcgtgccg tcctctcttc cgacgttgcc
     3061 gcatccggcg aacgctatac cgccgaacac ggtttgttcg gaaacgcaac cgagtggcac
     3121 cagaaaggcg gcgaagcctt tttcggcttt accgatgcct acggcaattc ggtcgaaact
     3181 tcctgccgcg cccgttaaac ggggcagcct gtttgaaccg ccgtccagat ttttcgggcg
     3241 gttttttgtg tcggacggac ggttttttgt gtcggatttg ttttgcagtt cggcctccgg
     3301 cagggttcgg gctgcctgat accgtccgta acttcgggta ttgccgagtt cgtcggcaac
     3361 ggccgctgcg gcttgggcgg cgttttgccg aacgcctgcg ttacttccct ttgtaggtcg
     3421 atctctttgg caaccgcgtc tttgtcaaag ctgtttttca gacggccttt tgtcaaatat
     3481 tatcggcagt ggctcaatgc caactttaaa cctgctccga tttcttcagg gctgttatcc
     3541 aatgataaaa tgacatcgtc tgcatcaatg gcattccacg cttccagctt gacatggcgg
     3601 ctcgggctga ttttcaggca gccgttgtgc agccaaatat ccacgctcat catgttttta
     3661 aatagggcgc gtctggtttt atagcccaag ttcccgcata gcttggcaac ccaatcctca
     3721 tagcgttgcc gaattttttc ggtatcaaaa aaatcttggt cttctggact gtcataaacg
     3781 aaagtcctgc tgtttgccaa cgcttgcaag actgtcgtgc ctaaagtttc attgtcggta
     3841 tccaatggca ggatatgggg ggatataggt ggtctattgc cgttaacccc aaacctgaac
     3901 atgtttgaac aatcagagtt tctttattag catcaaaaac tgcccaataa ttttgattct
     3961 gtttaaaaat cattatttga tctccgtaat tttgactgta tagtgga
//
LOCUS       DAXHNE010000159         3989 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.159, whole genome shotgun sequence.
ACCESSION   DAXHNE010000159 DAXHNE010000000
VERSION     DAXHNE010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.159"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(95..991)
                     /gene="argB"
                     /locus_tag="ACFM4Z_001826"
     CDS             complement(95..991)
                     /gene="argB"
                     /locus_tag="ACFM4Z_001826"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFC6021811.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRVPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1161..2291
                     /locus_tag="ACFM4Z_001827"
     CDS             1161..2291
                     /locus_tag="ACFM4Z_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HFC6021812.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDPQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVRERMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANVTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNVIGDLR"
     gene            2362..3213
                     /gene="lgt"
                     /locus_tag="ACFM4Z_001828"
     CDS             2362..3213
                     /gene="lgt"
                     /locus_tag="ACFM4Z_001828"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFC6021813.1"
                     /translation="MIIHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVIFGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDLEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3296..3958)
                     /locus_tag="ACFM4Z_001829"
     CDS             complement(3296..3958)
                     /locus_tag="ACFM4Z_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HFC6021814.1"
                     /translation="MDSQKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEDNGTIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
BASE COUNT          867 a         1169 c          983 g          970 t
ORIGIN      
        1 ttcaggaatg acggtttgga aattgcccga aaccccaaaa acagaaaccg gacaaacggg
       61 ttttccgcca aagccggcat tttccgactt tgcttcaggc atcttcccca ccgcccaaaa
      121 tcatcgaacc gataccggca tcggtaaaga tttccagcaa aagcgcgttg ggcaccctgc
      181 cgtcgatgat gtgcgtggct ttcacgccgt tgacggcggc ttcgaccgca gaagcgattt
      241 tcggcagcat accgccatac agcgtgccgt cggcaatcag ttcatcaatc cgtttcggcg
      301 tgagtttggt cagcagattg cccgttttgt ccatcacacc ggcgatattc gtcatcatca
      361 agagtttttc ggcgttcaat tcttccgcca atttgcctgc taccaaatcg gcgttgatgt
      421 tgaacgcttc gcctttttca cctacgccga cgggggcgac gacgggaatg cagccacgtt
      481 ctatcagccc tttaaccaaa cctgtatcga tgctttccac cgtaccgacc tgtccgatgt
      541 ctacgccatt ctgttcgggc gtatcgatca aaagtttctt cgccttaatg aaatggtcgt
      601 cgcgtccgct cacgccgacc gcgtgcccgc catatgtgtt aatcatcgac acgatttctt
      661 tattgacgtg cccgcccaac accatttcga caatatccat cgcctctttg tcggtaacgc
      721 gcattccttg gacaaactcg ccctttttgc cgactttttc aagcatcgca ttgatctgcg
      781 gcccgccgcc atgaacgatg acgggatgaa tgccgaccag cttcagcagc acgacatcgc
      841 gggcaaaccc ttctttcaag gcaggttcgg tcatcgcgtt gccgccgtat ttgatgacgg
      901 cgaccgaacc ggaaaaccgg cggatgtaag gcagcgcttc ggcaaggata cgcgctttgt
      961 cggcggcgga aataatgttt tcagactcca taacgactcc ctattgttga caatttcggc
     1021 ttatgttaac gccaaacgcc gtctgaaaca atcttcagac ggcatccggc agaccttcgg
     1081 gcaggttgaa gccttgcgat acgggcggta aaatcgcggt ttatccggtt cgagaaaccg
     1141 gcgacagcaa ggactttccc atgattaaaa tcagcacccg cttcgatgcc ggctcggtcg
     1201 tcgtcaaaga cctgaccgac ccttccaaca tccgcctcgc cctgcgtccc gacaacgcct
     1261 ccgattttgc acaatggttc tacttccggc tgcaaggcgc ggcatatcaa aactgcatca
     1321 tgcattttga aaacgcggca gaagccgcct acccgaaagg ctgggaaggc tatcaggcgt
     1381 gtgcctcata cgaccgccgc aactggttcc gcgtaccgac ttcctacgaa aacggcgtac
     1441 tgaccgtcaa tcatacgccg ctgtccaaca gcgtgtatta cgcctatttc gaaccttact
     1501 ccgaagaaca gcatttaaac ctcctcggcg acgcgcaagg cagcggcctg tgccgcatcg
     1561 acgacttggg cagcaccgtg caagggcgcg acatcaatct gctgaccatc ggcaatcagg
     1621 tcgaaagcga tctgaaaatc tggattaccg cccgccagca tccgggcgaa accatggcgg
     1681 aatggtttgt cgaggggctg ctcggcaggc tgctcgatcc gcaagacccg acagcgcgca
     1741 ccctgctcga ccgcgccacg ttctatatcg tccccaatat gaaccccgac ggttcggcac
     1801 tgggcaacct tcgtaccaac gccgccggtg cgaacctcaa ccgcgaatgg gaaaatccga
     1861 ctttggaaaa aagccccgaa gtgttcttcg tgcgtgaaag aatgctggaa accggcgtgg
     1921 atttgttttt ggatatccac ggcgacgaag ggttgccctt tgtctttgtt gcaggaactg
     1981 aaggcgtgcc gaactacaat ccgcgcattt ccgcgttgga aacacagttc aaaaccgccc
     2041 ttttgaacgc cagccccgat ttccaagatg aatacggtta cgaaaaagat gcgcccggtc
     2101 aggcaaacgt gaccttggca acaaactggg tcggcaaccg tttcaactgc cttgcctata
     2161 cgttggaaat gccgttcaaa gacaatgcca acctgcccga cgacgacttc gggtggaacg
     2221 gtcaacgttc gttgcgcctg ggcgaagccg cgttgtccgc catcctcaac gtcatcggcg
     2281 atttgcgctg aaacccttat tgcagaaatg ccgtctgaag catcatgcct ttcagacggc
     2341 atttgaaaac gaaagcctgt tatgattatc catccccaat tcgatcccgt cctcatcagt
     2401 atcggcccgc ttgccgtccg ctggtatgcc ctaagctaca tcctcggatt tattcttttt
     2461 acctttctcg gcagaaggcg catcgcgcaa ggcttgtccg tttttaccaa agaatcgctt
     2521 gacgacttcc tgacatgggg cattttgggc gtaatttttg gtgggcgttt gggttacgtc
     2581 ctgttttaca agttttccga ctacctcgcc catccgcttg atattttcaa ggtatgggaa
     2641 ggcggaatgt cgttccacgg cggctttttg ggtgtagtta ttgccatatg gttgttcggc
     2701 cgcaaacacg gcatcggctt cctcaaactg atggacacgg tcgcaccgct cgttccgctg
     2761 ggtctcgctt cgggacgtat cggcaacttc atcaacggcg aactttgggg acgcgttacc
     2821 gacatcaacg cattttgggc aatgggcttc ccgcaggcgc gttacgaaga cctcgaagcc
     2881 gccgcgcaca atccgctttg ggcagaatgg ctgcaacaat acggtatgct gccgcgtcat
     2941 ccctcgcagc tttatcagtt tgcacttgaa ggcatctgcc tgttcgccgt cgtttggctg
     3001 ttctctaaaa aacagcggcc gaccggacaa gtcgcctcgc tcttcctcgg cggctacggc
     3061 atattccgct tcattgccga atttgcacgc caacccgacg actatctcgg gctgctgacc
     3121 ttggggctgt cgatggggca atggttgagc gtcccgatga ttgttttggg tatcgtcggc
     3181 tttgtccggt tcggcatgaa aaaacagcac tgaccgcctg tgaaaaatac cgtctgaaac
     3241 atggaaatcc ggtttcagac ggcattttta cgtttggttc atggaaacaa acgcttcaat
     3301 tcttaccgca acacgccttg tattttctgc cgctgccgca cggacaggga tcgttcctgc
     3361 ctgttttttc gccttccctg cggacggttt gcggcttgtt gatgaccgcc tgccagtagc
     3421 ggtagatatc cgccaatgca taaggcaact cggattccag ttccgccagc tcgccttctg
     3481 tgaattgcag acggatagtg ccgttgtcct cttcgtcgta aataccgccc aatgccatga
     3541 tgggataaaa caactcttca aacgcttcat catcgacggc ttcaaaccaa tcggtcggca
     3601 caatatccaa accgtaaaga taagcattgc accatgtgta aaaatcgctg ccgccgtctt
     3661 cgttttcata cagccacaaa tcgggcagtt ttttatccga catcgcggcg gttgtttcca
     3721 tcgccattgc caaaaccagc cgttcgattt cggaacgttc ggcggcggta aattgcgatt
     3781 cgtcgcccaa cacttcgggc agccagtcga gcggtgtcaa tttgtccggc ccgctcaaca
     3841 gcgccgtcat aaaaccttga acctcgtcgc aacgcatcgt gttgccttgt tcgcttttgg
     3901 catccaacaa ttcgctcaac cgccgtttgg atgcttcggt aaatttttgg gaatccatca
     3961 ttttcctttt caaatgggtt ttgcgccct
//
LOCUS       DAXHNE010000160         3872 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.160, whole genome shotgun sequence.
ACCESSION   DAXHNE010000160 DAXHNE010000000
VERSION     DAXHNE010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.160"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..454
                     /locus_tag="ACFM4Z_001830"
     CDS             <1..454
                     /locus_tag="ACFM4Z_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC6021815.1"
                     /translation="YKTPAARPMQTLDGEMAGGNKPPKSITSGGKANAATYPQLVNQL
                     TGQNLKNIAAQDPRLSLALHKSEKNFPIGTATYEEADRLGKIWVSEGARQTSGGGWLS
                     IDGTRQYRPPTEKNSQFATTGIQANFETYTIDSNGKRNKIKNGHLNIR"
     gene            459..818
                     /locus_tag="ACFM4Z_001831"
     CDS             459..818
                     /locus_tag="ACFM4Z_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC6021816.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            895..1107
                     /locus_tag="ACFM4Z_001832"
     CDS             895..1107
                     /locus_tag="ACFM4Z_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021817.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            1204..2166
                     /gene="mafB"
                     /locus_tag="ACFM4Z_001833"
                     /pseudo
     CDS             1204..2166
                     /gene="mafB"
                     /locus_tag="ACFM4Z_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            2163..2534
                     /locus_tag="ACFM4Z_001834"
     CDS             2163..2534
                     /locus_tag="ACFM4Z_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6021818.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            2640..2969
                     /locus_tag="ACFM4Z_001835"
                     /pseudo
     CDS             2640..2969
                     /locus_tag="ACFM4Z_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            2949..3389
                     /locus_tag="ACFM4Z_001836"
     CDS             2949..3389
                     /locus_tag="ACFM4Z_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB"
                     /protein_id="HFC6021819.1"
                     /translation="MAGGNKPPKPSTQQHPTHSDNNIGLPASYVKPDTSISPTGTIQD
                     RIRWTKSKFPTEKSLNGHFKAHGKEFGDITIEDYQKMASDLLSKQTSDKILGYQTEHR
                     RVRYDINNNIYVLANPKTFKIKTMFKPNLGKKYYDGEFKKDMGN"
     gene            3355..3561
                     /locus_tag="ACFM4Z_001837"
     CDS             3355..3561
                     /locus_tag="ACFM4Z_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPCC family cysteine-rich protein"
                     /protein_id="HFC6021820.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            3582..3848
                     /locus_tag="ACFM4Z_001838"
                     /pseudo
     CDS             3582..3848
                     /locus_tag="ACFM4Z_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224185.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1375 a          645 c          788 g         1064 t
ORIGIN      
        1 ttataaaaca cctgctgccc gacctatgca gactttagat ggggaaatgg ccggtgggaa
       61 taaaccgcct aaatctataa cgtctggagg aaaagccaat gctgcaactt atcctcaatt
      121 agttaatcaa ttaactgggc aaaacttaaa aaacattgcg gctcaagatc caagattgag
      181 tctagctctt cataagagtg aaaaaaattt tccaatagga actgcaactt atgaagaggc
      241 agataggcta ggtaaaattt gggttagtga gggtgcaaga caaactagtg gaggcggatg
      301 gttaagtata gatggcactc gacaatatcg gccaccaaca gaaaaaaatt cacaatttgc
      361 aactacaggt attcaagcaa attttgaaac ttatactatt gattcaaatg gaaaaaggaa
      421 taaaattaaa aatggacatt taaatattag gtaattatat gagattaaaa ttaaaaaatt
      481 atgattgttc tgactttgat ttggaagatt tcccacaaga caagttagaa aatttttgta
      541 tacttttgac attatcaata ggttttgatg aaagtaatgg tgctgattat ttttatgttt
      601 atatatatag cactgaatgg ttactttcaa atatacatag accgatgtca ttaaaaaact
      661 caattgttac aaaccgtttt aatattgaac acatattaaa attgataaat gatatattgg
      721 aaatatgtaa ttcaacgtct gaagataagt ctatatcaaa tttggcaaaa tattttgact
      781 gggaatttga cgattataat ctaaatatcc aagactaaag atggtgggac agcatttaga
      841 ccaaaaacag gcaaacggta ttttgatgac gaattaaaat aaaaggaatt attcatgaaa
      901 ttaattaatt ttgaagagag ttcagttaaa attagtctcg ataaagatga gttgtatatt
      961 attcaagcaa ttgtaggaga aatttattca ggcgtatgcg tggattgtag agattttgag
     1021 ataattcatg gtgttgaaaa aataaagttc tttctttgga taaagaactt aaaaaaatat
     1081 atgatacttg ggataaatgt tagctaggta aggtgggcac ttgttgccca ccttttgccg
     1141 tatattaaaa atccttttca ggctgcctga aactgggtgg gcatcaatgc caatcctaac
     1201 tgtgctgatg aagcaggaaa actgatatgg gaaaacgacc ccaataaaaa ttggtgggct
     1261 aaccgtatgg atgatattcg cggcatcgtc caaggtgcgg ttaatccttt tttaatgggt
     1321 tttcaaggag tagggattgg ggcaattaca gacagtgcgg taagccctgt cacagataca
     1381 gccgctcagc agactctaca aggtattaat gatttaggaa atttaagtcc ggaagcacaa
     1441 cttgctgccg cgagcctatt acaggacagt gcttttgcgg taaaagacgg catcaattcc
     1501 gccagacaat gggctgatgc ccatccgaat ataacagcaa cagcccaaac tgcccctgcc
     1561 gtagcagagg ccgcaggtac ggtttggcgc ggtaaaaaag tagaacttaa cccgactaaa
     1621 tgggattggg ttaaaaatac cggctatgaa acaactgtta cccgaaagac tttagatggg
     1681 caaatagctg gcaagaacaa gcctattaaa ccttcttcag gagattatgt cttgccggta
     1741 ggcagacaaa tcatagatcc tactcgtaca tctttttcac aagctacagt tagctatcag
     1801 aagcgtggtg caaattacaa ctatgattct cttgtagctg caatgaacga aaaaaaatca
     1861 tgggttggcg acagggttga tgttgttaat atgcctgatg gagcacctac tagtatggat
     1921 aacacgcgta ttatggcagc acgtgaagca ggagtaaaag tggaagcgaa tgttcataat
     1981 tttaatgacc gattatcatc aaaagagaga atcaggttta agcatgatgg tattgagcct
     2041 caaacttggg gagaagctat ccagctacga attagaaagc aagaaacaca aaaaggagtt
     2101 ccagaagggt ggagcaaaag atttcctaac ggaagtattt atgatgtaaa ggtacttagg
     2161 aaatgataaa acaaaatagt tttgttccgt atcctgaagc aatgcttcct aaaggattta
     2221 aatatccgca aagttattta aaattagctc aatccactca tgccattaac tacgatgaac
     2281 aatattcttt tccttggtgg tttgaaaatg cagaaagcaa tatatcagaa gtaattgaca
     2341 tttattttga aataactggc attccaaacc tattaccttt tgctagaaac caagagtggg
     2401 ctgcctgttt tgatatttca gataaatcag gtaatcctaa aattatagta gttaatttag
     2461 ataatacaaa atattacgag acttttgaaa attttgatac ttggctaaaa gaagctgaaa
     2521 atgatggttg gtagcagccg taggtcggat tctcgaatcc gacattttcc aacagcggca
     2581 tttcggaaac gatagacgcg tcaaatattt ttgtcggata caaatatccg acctacatct
     2641 ctgcgcagca aactttacaa gatattaatg aattaggaaa tttaagtccg gaagcacaac
     2701 ttgctgccgc gagcctatta caggacagtg cttttgcggt aaaagacggc attaattccg
     2761 ccagacaatg ggctgatgcc catccgaata taactgcaac agcccaaact gccctttccg
     2821 tagcagaagc cgcaactacg gtttggggcg gtaaaaaagt aaaccttaac ccgaccaaat
     2881 gggattgggt taaaaatacc ggctataaaa cacctgctgc ccgccctatg cagactttag
     2941 atggggagat ggcaggtggg aataagccac caaaaccaag tacgcagcaa caccctacac
     3001 actctgataa caatatcggc ttacctgcct catatgttaa acctgataca tctatttctc
     3061 cgacaggaac aattcaagac cgcatcagat ggacaaagtc caagtttcct actgagaaat
     3121 ctttaaatgg acatttcaaa gctcatggaa aagaatttgg cgatataacc attgaagact
     3181 accaaaaaat ggcgtctgat ttgttatcaa aacagacatc ggacaagata ttaggttatc
     3241 agacggaaca tagacgagtg cgctatgata tcaataacaa tatctatgtt ttggccaatc
     3301 caaaaacatt caaaatcaaa acaatgttta aaccaaactt aggaaagaag tattatgatg
     3361 gagaattcaa aaaagacatg ggaaattgac ggagaaatat ggctacattg tcctgtttgc
     3421 ggaactgaag ttatggacta tgatatctgt gacgtttgtc agtggcaaaa tacaggagaa
     3481 actaatatag atggtggccc taatgaaatg acacttgcgg aggcgaaaga agcttacgca
     3541 aaaggcttac caatcagata aataagcacc tagagaaatc aatgatgacg gaatcccatg
     3601 gttacctatc aaataattta cctgttaaaa tcataaatga tattatttat gcaacccagt
     3661 tagtcgaaga tttggtttta ggaaaaataa aaattgttga ttttttaaaa tcatataaca
     3721 atttttattg ttggcttggt tttgatgagt tgcctcaatc tgagaaaata aaattcctaa
     3781 gctatcttaa tatattaagt attcataaag aaatacaaga tgaaactgtg aatagggttt
     3841 ataccgattg aaaaatagta gatagagatt aa
//
LOCUS       DAXHNE010000161         3859 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.161, whole genome shotgun sequence.
ACCESSION   DAXHNE010000161 DAXHNE010000000
VERSION     DAXHNE010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.161"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="ACFM4Z_001839"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="ACFM4Z_001839"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFC6021821.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLERFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="ACFM4Z_001840"
     CDS             1439..1924
                     /locus_tag="ACFM4Z_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFC6021822.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="ACFM4Z_001841"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="ACFM4Z_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFC6021823.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKITGQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGTKPKRIKYKPVTR"
BASE COUNT          784 a          945 c         1076 g         1054 t
ORIGIN      
        1 gaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cttcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 tcgccagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataag
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgccga tgtcaaacct
      661 gaggacttgg cttggaaggt tttggccgtc aatatttcag atatggcggc gatgggcgcg
      721 ataccgcgtt gggtgttgtt aagtgcggct ttgcccgaat tggatgaggt atggctggaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcattgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgcccgggc ggcgcaggat gtttcagacg gcctcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaggttttag atgccgacgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacga ccgttgtgtt ttttggcttt
     1501 tggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggca tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgtggg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgt ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgc gttaccggtt tgtatttgat gcgtttcggt ttcgtgcctt cttcgccgag
     2041 tcggcgtttc ttgtcggctt cgtattcctg atagttgccg tcaaagaaca cccatttgga
     2101 gtcgccttcg caagccaaga tatgcgtagc aatacggtcg aggaaccagc ggtcgtgcga
     2161 aatcaccatc acgctgccgg caaattccag caatgcgtct tccaacgcgc gcagggtttc
     2221 cacgtcgagg tcgttggacg gttcgtccag cagcaacaca ttgccaccgc ccaacaaggt
     2281 ttttgccaag tgcaaacgtc cgcgttcgcc gccggaaagc tgccccgtga ttttgctttg
     2341 gtcgctgcct ttgaaattga agcgtcccaa atattggcgg gcggggattt caaactgccc
     2401 gacctgtaaa atatcgcgac cttcggcaat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaagctcat tttcacggtt tgcccgattt tcacttcacc
     2521 ggaatcgggc tgctctttgc ccgcaatcat tttaaacagt gtcgatttac ccgcgccgtt
     2581 cggaccgatg atgccgacaa tcgcgcccgc aggcactttg aagctcaaat cgtcaatcag
     2641 cactttgtcg ccgaacgatt tggaaacatt cacaaattca atcacttcgt tacccaaacg
     2701 ctcggcgacg ggaatgaaga tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcttca aaacgagcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcatcgc cttcacgcgc gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatggccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgtgttacc gcaacgactg taccggggaa gcgcacgaga aattgctcca gccactcgac
     3061 agattccgcg tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gcttgctcaa
     3121 caagagtttg cacaaagcga cgcggcgttt ttcaccgccg gacaaattat cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc agcgatttcc aattcgtgtt ccgcaccgcc
     3241 gcccgtggac gaacccgccg caataatcgc ttccaaacgc ccctgctctt ccgccaacgc
     3301 gtcaaaatcc gcatcgggat tggcgtactc ggcatacact tcctccaaac gtttctgcgc
     3361 ggcagccact tcgcccaaac cgctttccac ttcctcacgc acggtttttt ccggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt aataccgccc atcggcacgg cttcgccctc
     3481 aaattcttta tccacgcccg ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcaaa ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgctcacgcg cagcatagaa tagacgtatt gttgggacat
     3661 ggttttctcg ttttcatcaa acaaatttca gacggccatt ttaaccgata atttgattta
     3721 agccagttta tccgcgaacc ggtattgcca aaatcgggca ggattcataa aatccgctta
     3781 tccctttgaa attatataga caaaaaaata ataatgatag gggatcgccg caccggcaac
     3841 catttcggat tttccaaag
//
LOCUS       DAXHNE010000162         3848 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.162, whole genome shotgun sequence.
ACCESSION   DAXHNE010000162 DAXHNE010000000
VERSION     DAXHNE010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.162"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(33..1943)
                     /locus_tag="ACFM4Z_001842"
     CDS             complement(33..1943)
                     /locus_tag="ACFM4Z_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6021824.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGLQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGGKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFHPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     VFEGQGRLKEYIGGYQDYIDAKSREDKIQTASAPKASDAEPAKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINTQLSYPEIFKDYEKAGALQNRAEEIEMLLLEKLERW
                     ELLETKRNGNAV"
     gene            complement(1962..2606)
                     /locus_tag="ACFM4Z_001843"
     CDS             complement(1962..2606)
                     /locus_tag="ACFM4Z_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC6021825.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            complement(2771..3589)
                     /gene="opc"
                     /locus_tag="ACFM4Z_001844"
                     /gene_synonym="opcA"
     CDS             complement(2771..3589)
                     /gene="opc"
                     /locus_tag="ACFM4Z_001844"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HFC6021826.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHISVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWLAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTNLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
BASE COUNT          941 a          875 c          966 g         1066 t
ORIGIN      
        1 tcgtttgaac atttttttct tcctgtttga tttcagacgg cattgccgtt ccgtttggtt
       61 tccagcagct cccagcgttc cagcttttcc aaaagcagca tttcgatttc ttcggcgcgg
      121 ttttgcaatg cacctgcttt ttcgtaatct ttgaaaattt caggatagga aagttgggta
      181 ttgatttcag cctgctcggc ttccaaagcg gcgatttcgt cgggcagggc atcgagttcg
      241 cgctgctctt tgtaggacag tttgaccgtg cggttggctt tgggtttttc tttggcgggt
      301 tcggcatcgg atgctttggg tgcggatgcc gtctgaattt tatcttcccg cgattttgcg
      361 tcgatatagt cctgatagcc gccgatgtat tctttcagac ggccttgtcc ttcgaaaaca
      421 atgctttggg taattacgtt atcaaggaac atacggtcgt gcgagacaag gaatactgtg
      481 ccttgataat cgcgcaacag gtcttcgagc agctcttggg tgtcgatgtc taagtcgttg
      541 gtcggttcgt ccaagaccag gatattggca ggacgggtaa agagttttgc cagcaaaagg
      601 cggttgcgtt ctccgccgga gagcgatgaa acagggcttt gcgcacgggc tggatggaac
      661 aggaaatctt ccaaatagct catgacgtgt tttttcttac cgccgacttc aacgtaatcg
      721 tttccctgtc cgagggtgta aaacacggtg tcgttttcat tcaacgcgct gcggaactgg
      781 tcgaaatagg cgacttcctg cttactgccg atacggattc tgccgtaggt cggctgcaat
      841 tcgcccaaaa tcagcttaag gaaggtggtt ttgccgatgc cgttggggcc gattaggccg
      901 attttgtcgc cgcgctgcaa gatagcggag aatttgtcca taatgacttt gccgccatag
      961 gcaaacgaag cgtgttccaa ttcggcgatg attttgccac ttttctcacc gctatcgagc
     1021 ttgaagttga cttgtccctg tacgttgcgg cgttctgcac gctggcggcg cagctcttcc
     1081 aaacggcgca cgcggccttc gttgcgggta cggcgcgctt cgatgccttt gcgtatccat
     1141 gcttcttcct gtgcgtggaa tttgtcaaag aggcggttgt gttccgcttc gactgccaac
     1201 tcttgcgctt ttttctcgct gtatttagag aacgagccgg gataggaacg caaaataccg
     1261 cgatcgagtt cgacaatccg cgtggcgata ttgtccaaaa aacggcggtc gtgggtaatc
     1321 acaaccaagc tgccttcaaa cgctttgagc agattttcca gccaaataat cgcgtcgata
     1381 tccaaatggt tggtcggctc gtccagcagc aatacgtcgg gcttttgcac ccaagcctga
     1441 gccaaggcga cgcgcttttt ctgaccgccg gaaaggttgc cgattttttc attttccggc
     1501 aaaccgagtt cccccaaagt ctgcttgact gccgcatcca gtttccagcc gtccttcgct
     1561 tcgatttcaa gttgcaattc gttgagttct ttcaacaaag cctcactcga accattttcc
     1621 aactcatggc tgacatgatg ataacggcgc aataaatcac gaatttcgcc caaaccttcg
     1681 gcaacggtat caaatacggt tgcgtcctta tcaaaaaagg attcctgcgg tacataaacg
     1741 attttgaggt tgttttgaac aataatctgc ccgtcgtcga gcttttgcaa accggcgagg
     1801 atttttaaaa acgaagactt gcctgcgccg ttgcgtccga ttaagccgac tttttcgccg
     1861 ctgtcgagtt gaaaagaagt tttgtcgagc aaggcaacgt ggccgatggc aaaagaagcg
     1921 ttttctacag ataatatatt catgatacaa attctcaaca gttaccgttt ggattttacc
     1981 gcaagtttgg cgcgggcaat ttcaaccgca cccggcagga cggaaacaat aatgatgccg
     2041 cccatcacca agcccagatt gttttttacg acggggaagt tggcaaagaa atagcccgcg
     2101 taagaaaaca ggataaccca caacaagcca ccgatgatgt tgtagcggat aaatttggca
     2161 tagtgcattt tccccatacc ggcgacgaag ggggcgaagg tgcggacgat gggcataaaa
     2221 cgggcaatga tgatggtttt gccgccgtgt ttttcgtaaa aacggtgggt tttatcgaga
     2281 tattcacgtc ggaagatttt agaatcgggg ttggcgaaca gcctgccgcc gaaatatttg
     2341 ccgacggtaa aattgagcgc gtcgccgagt atggcggcaa ggcttaataa tgcaaccatc
     2401 aaatgaatat ccataccgcc cagcgcggca atcccgccgg cggcaaacag cagcgaatcg
     2461 ccgggcagta agggcgtaac aatcaggccg gtttcgcaaa aaacaatcaa aaacagaatc
     2521 gcataaatcc acacaccgta ttgcgccgac agcgcgagca ggtgttggtc gatatggagg
     2581 atgaagtcga tggcggaggc aagcacggcg cgttcctaaa aaaaacaaac cgcgtatttt
     2641 aaccgattgg aaaaatgccg tctgaaaagt ttcagacggc atcggctatt caaattcatt
     2701 tcacgtaaaa accgcaaacc aaaatagttt gcggtttggc atttaaagtg acaatgatga
     2761 tttcaaatca tcagaatttt atgccgacgc gcaagccgta ttcacgaata ctggttttcg
     2821 ggatggtgag cgatacgtcg ccactcttgg ttgttacact aaactcgccg gattctttgt
     2881 aagtgcgttg tttgtagaac ggccccgcct cgatgctggc ggattcgccc agttttttac
     2941 caatatttgc acccaatcca aagccccagc cgttggtttt ttgattgatg tcttttttga
     3001 gattggtaac gccggtgttt aatttatagc gggaattgag gtcaaatttc acttcagacc
     3061 aagggttgat ataccagccg ttacccagtt gggaaagcaa atccgcgtga actttggcta
     3121 accacgactg acggctgctg tgaagcgtat gcttggtggt tttaatgctg tcttttgaag
     3181 attcaaaacc caagccggca cccacacgga aatttaaaga atcacttaac gtttgggtgt
     3241 aggtgtagcc tgtgtaaaga tcgatacggt tttcaggaac gccggtgggc agttttacat
     3301 ttttagtctt acccagcttg ttctcatctg tttccaaatt aataatattt tttttgctgc
     3361 gcccgaaacc ggcttccaag cggatgcctt ggttggcatc aaaaggaata tcagcacgca
     3421 cgctgatgtg tttggcagct ttgtgttttt ctttcaggaa agcacgagtt gaagaaatgg
     3481 aagagaggtc ggtgtggacg gtaaactcat tagcggtttg aagctcttgt gcagcggcgg
     3541 cagtaccggt cagggcaatc atggcacatg taaaaactgt ttttttcata gttaaaacct
     3601 ctaaaatttg gattgtagtc ggatatggta acataacgta aataatcgtt acgcttacaa
     3661 ttatattctt aagctttcgg gggggatttt acatatatta ataaaaatta acaaatagtt
     3721 atttgtttac aacgaattgt tattctcact tggttttctg ttttttatgg gaatgacgaa
     3781 attttagttt gtgtgtattt atcggaaaaa cagaaacccg ccgccgtcat tcccgcgcag
     3841 gcgggaat
//
LOCUS       DAXHNE010000163         3846 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.163, whole genome shotgun sequence.
ACCESSION   DAXHNE010000163 DAXHNE010000000
VERSION     DAXHNE010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.163"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            270..1391
                     /gene="dnaJ"
                     /locus_tag="ACFM4Z_001845"
     CDS             270..1391
                     /gene="dnaJ"
                     /locus_tag="ACFM4Z_001845"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFC6021827.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKLGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKDPCIKCHGEGRNKTGKAVEVN
                     IPAGIDDGQRIRLSGEGEPGRHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTIPKETQTGRRMRVKGKGVKSLRSSAIGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1586..3604
                     /locus_tag="ACFM4Z_001846"
     CDS             1586..3604
                     /locus_tag="ACFM4Z_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HFC6021828.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDDRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPATERAEQDLSPKAMIFWVLSMMFILGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIAADLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMIIFARRVLKAGR"
     gene            complement(3648..>3846)
                     /locus_tag="ACFM4Z_001847"
     CDS             complement(3648..>3846)
                     /locus_tag="ACFM4Z_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HFC6021829.1"
                     /translation="VLSDTTELTYKCTDYYKPEAEQVLIWNDPAIGIGWPLQTAPLLS
                     PKDLAGKTWAQAEKLRLTLSR"
BASE COUNT          827 a          827 c         1135 g         1057 t
ORIGIN      
        1 aatagggggg aagtctgcag tcgcatcaaa tgccgtctga acatgcgttc gggcggcgtt
       61 tttataacaa aaacacttca tggcggttgg ttttatgcct gtctaagttt ttgtgtcgtg
      121 catacctgaa gatttcagac ggcatcggtt tatgctgtct gaaaagtgta ttccgtttca
      181 gtttgtaagc tatggcagtc tgtttgtctt gtgttttgcg caattgccct tattttgagc
      241 cgtgatttta ttttgaatta gatgaaaaaa tgagtaatca agatttttat gcgacgctgg
      301 gtgtggcaag aacagctacc gatgatgaga ttaaaaaagc ctaccgtaaa ttggcgatga
      361 aataccatcc cgaccgcaat cctgacaata aagaggcgga agagaagttt aaagaagtac
      421 aaaaggcgta tgaaactttg tccgataaag aaaagcgtgc tatgtatgat caatatggtc
      481 atgcggcgtt tgaaggcggc ggacaggggg gcttcggagg gtttggcgga tttggtggtg
      541 ctcaaggttt tgactttggg gatattttca gccaaatgtt tggaggcggt tcggggcgtg
      601 cccagcctga ttatcagggt gaggatgttc aagtcggtat cgaaatcacg cttgaagaag
      661 cagcaaaagg cgtgaagaaa cgcatcaata ttccgactta tgaagcgtgc gatgtctgca
      721 acggcagcgg cgcgaaactc gggacatccc ctgaaacctg tccgacttgt aaaggctcgg
      781 gtacggtgca catccagcag gcgattttcc gtatgcaaca gacttgtccg acttgtcatg
      841 gtgcaggtaa gcatattaaa gacccttgta ttaaatgtca tggggaagga cggaataaaa
      901 ccggcaaggc tgtagaagtc aatattcctg caggcataga tgacggacaa cgtatccgtt
      961 tgagcgggga aggcgagccg ggaaggcatg gtgcgcctgc aggcgatttg tatgtaacgg
     1021 tgcgtatcag ggcccataag attttccaac gcgacggtct ggatttgcat tgcgaactgc
     1081 cgattagttt tgccacggct gctttgggcg gggagttaga agtgccgact ttggacggca
     1141 aggttaagct tactattcca aaggaaaccc agacaggcag aaggatgcgt gtgaaaggta
     1201 agggtgtcaa atcgttgcgc agtagtgcaa tcggtgattt gtactgccat attgttgttg
     1261 agacgcctgt taatttgacc gaccgtcaaa aagagctttt ggaagagttt gagcggattt
     1321 ctaccggctt ggaaaaccaa acgccgcgca agaaatcgtt tttagacaag ctgcgcgatt
     1381 tgtttgattg attttaaggt tcggaaacaa gcagctgtat cggggaatct ccctgatacg
     1441 gctgtttttg cttgtttgga aatagttttt attttcaatg gggtatgggc agggtgggat
     1501 aactgttttt aaccgttctt tttaaaactt gacatcatgg cgtgatgcca acaatatgtg
     1561 aacgtctgtt gtcaaaggaa gaataatgaa taaatcttta tccggttcgg tagaagaata
     1621 ccgcgaactg acgctccgag gcatgatact cggcgcattg attactgtaa tttttactgc
     1681 gtccaacgtt taccttggtt tgaaagtcgg gctgaccttt gcctcgtcga ttcccgcagc
     1741 agtgatttcg atggcggttt taaagttttt caaaggcagc aatattttgg aaaacaacat
     1801 ggtgcagacc caagcctcgg ctgcgggtac gctttcaacc atcatcttcg tcctgcccgg
     1861 tttgttgatg gcgggctact ggagcggttt cccgttctgg cagacgacgc ttttatgtat
     1921 tgccggcggg attttggggg tgattttcac cattccgttg cgttacgcga tggtggtgaa
     1981 aagcgatttg ccttatccgg aaggtgtggc ggctgctgaa attttgaaag tgggcggtca
     2041 tgaagaaggg gatgaccgtc agggcggcag cggcatcaaa gagctggcgg caggcggcgc
     2101 gttggcggga ttgatgagct tttgcgccgg aggtctgcgt gtgattgccg acagcgcgag
     2161 ttattggttt aaaagcggta cggcaatttt ccaactgccg atgggctttt cactggcctt
     2221 gttgggcgcg ggctatttgg tcggactgac gggcggcatc gctatcctgt tgggcatttc
     2281 gattgcttgg ggcattgccg tgccgtattt ctcctcacac attccgcaac cttccgatat
     2341 ggaaatggcg gcgtttgcga tgaagctgtg gaaggaaaaa gtgcgtttta tcggtgcggg
     2401 aaccataggt atcgcggcgg tttggacgct tttgatgctg ctcaagccga tggtggaagg
     2461 catgaagatg tcgttcaaga gttttggcgg cggtgcgccc gctacagaac gcgctgaaca
     2521 ggatttgtcg cctaaagcca tgattttctg ggtattgtct atgatgttta ttctaggcgt
     2581 gtcgttttac cactttatcg gcgattcgca cattacgggc ggcatggctt ggcttttggt
     2641 ggtcgtttgc acgcttttgg cttccgtcat cggctttttg gtcgccgccg cctgcggtta
     2701 tatggcaggt ttggtcggct cgtcttccag cccgatttcc ggcgtgggca tcgtgtccgt
     2761 cgtcgttatt tcactggttt tgctgctggt aggcgaatcc ggaggtttgt tggcggatga
     2821 ggctaaccgc aaatttttgc tggcactgac tttgttttgc ggctcggcag taatctgcgt
     2881 ggcttcgatt tccaatgaca acctgcaaga cttgaaaacc ggctacctgc tcaaagccac
     2941 gccttggcgg cagcaagtcg ccctgattat cggctgtatc gttggtgcgc tggttatttc
     3001 gcccgtgttg gaactgcttt acgaagccta cggctttacc ggcgcaatgc cgcgcgaagg
     3061 catggatgcg gcgcaggctt tggcagctcc tcaagcgact ttgatgacga ccatcgcgtc
     3121 gggcattttc gcccacaacc tcgaatgggt ctatatcttt accggcatcg cgattggcgc
     3181 ggtgttgatt gccgccgatt tggtgttgaa aaaatcatca ggcggcaaac ttgccctgcc
     3241 cgtccttgcg gtcggtatgg gtatttatct gccgccgtcc gtcaatatgc ccatcgtggc
     3301 aggcgcggtg ttggcggcgg tgttgaaaca catcatcggt aaaaaagcgg aaaaccgcga
     3361 aggccgtctg aaaaacgccg agcgcatcgg aaccttgttc tccgccggcc tgattgtcgg
     3421 tgaaagcctg attggtgtga ttatggcgtt tattattgcc ttctccgtga ccaacggcgg
     3481 ctcggatgcg ccgctcgcgt tgaatctgca aaactgggat gccgccgctt cttggctggg
     3541 cttggcgttc ttcgttaccg gaatgattat ttttgcacgg cgcgtactga aggcgggtag
     3601 gtagcctgtc ggagaaaatg ccgtctgaaa cgttcagacg gcatttttta tcgggaaagt
     3661 gtaaggcgga gcttttcggc ttgcgcccac gttttgccgg caaggtcttt gggcgacagc
     3721 agcggcgcgg tttgaagcgg ccagcctatg ccgattgccg ggtcgttcca tattaaaacc
     3781 tgttcggctt caggcttgta atagtccgtg catttgtagg taagttcggt cgtgtcgctc
     3841 aaaacg
//
LOCUS       DAXHNE010000164         3804 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.164, whole genome shotgun sequence.
ACCESSION   DAXHNE010000164 DAXHNE010000000
VERSION     DAXHNE010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.164"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(38..604)
                     /locus_tag="ACFM4Z_001848"
     CDS             complement(38..604)
                     /locus_tag="ACFM4Z_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HFC6021830.1"
                     /translation="MGFYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWVAFVLLGGLGLKMMREGLSGEAEDVRESKRES
                     LWMTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVMVAVGLTTGRALGVLF
                     GRCAEFAGGLVLIAIGTWTLLSHLGLIQ"
     gene            complement(632..925)
                     /locus_tag="ACFM4Z_001849"
     CDS             complement(632..925)
                     /locus_tag="ACFM4Z_001849"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021831.1"
                     /translation="MPSETSFDRFRRHFHTPPPPFQWAFFIKRKISRHSHESGNQGLG
                     VGETVLSDKFPHRQLWIPACAGMTGFLFLIFCFCGNDGMRVFVRHFCIFLLLL"
     gene            complement(1001..2515)
                     /gene="katA"
                     /locus_tag="ACFM4Z_001850"
     CDS             complement(1001..2515)
                     /gene="katA"
                     /locus_tag="ACFM4Z_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HFC6021832.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
     gene            2863..3714
                     /locus_tag="ACFM4Z_001851"
     CDS             2863..3714
                     /locus_tag="ACFM4Z_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HFC6021833.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDPDEFSDAGFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QEAACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLQHALTALKKFDPAGVAA
                     ADLNESLILQIERLGECAAKPAALHIVRNALDSIDGNRSQTLARIKKHLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESFEIPAA
                     HKRKTAE"
BASE COUNT          879 a         1053 c          996 g          876 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactatattt tccgacatca ttgaatcaaa cccaaatgcg
       61 acaagagcgt ccatgtgccg atggcaatca acaccaaacc tccggcaaat tccgcacacc
      121 tgccgaacaa tacgcccaaa gcccttcccg tcgtcagccc gaccgccacc atcaccgtcg
      181 tcgccatacc gatgattgcg gcggcaaagg cgatgtttac ctccataaac gccaagccca
      241 ccccgactat catggaatca atactggttc caaaagcagt caaaaccgtc atccataggc
      301 tttcccgttt gctttcgcgc acatcttccg cctcgccgga cagcccttcg cgcatcattt
      361 tcagacccag cccgcccagc aggacgaaag ccacccaatg gtcccattcg ctgataaacg
      421 gcttggcata aaaaccgcct acccagcctg ccagcggcgt gagcgcttca accgtgccga
      481 acaccaaagc cgttgccgca attttgcgcg gaggcattct gaccgccgca ccctttgcca
      541 atgcgacggc aaacgcatcc atcgacatcc ccagagcaat caagagcaaa gcataaaaac
      601 ccataccgca cccgtcctca aaaagggcgg attatagcaa aagcaaaaaa atgcaaaaat
      661 gccgcacgaa aacccgcatc ccgtcattcc cgcaaaaaca aaaaatcaaa aacagaaatc
      721 ccgtcattcc cgcgcaggcg ggaatccaga gttgtcggtg cggaaactta tcggataaaa
      781 cggtttctcc aaccccgagt ccttgattcc cactttcgtg ggaatgacgg gatattttgc
      841 gtttaataaa aaacgcccac tgaaacggcg ggggtggggt gtggaaatgc cgtctgaagc
      901 ggtcaaacga cgtttcagac ggcattttta tgcccggtta tttccggtag cggacggcgc
      961 gggacaggat ttcttcaatt tccatccaca taatgccccc ttacagcaaa ccgggctggc
     1021 cgagtgcggg gtcggtcgcg cgggcggctt gtgcgtcttc gacagtcagt ccgagggctt
     1081 ttgccacgcc ttcgccgtat gccgggtcgc aacggtagca gttgcggata tggcggtatt
     1141 tgatgaagtc gggcgcgtcg cccattgcgg cggcggtgtt gccgaacaat gcctgtttct
     1201 gcgcgtcgtt catcaggttg aacagggcgc gcggttggct gaaatagtcg tcatcgtctt
     1261 ggcggtagtc ccagtgtgcc gcgtcgccgt tgattttcaa aggcggttcg gcgaagtcgg
     1321 gttgttgctg ccattggccg aagctgttgg gttcgtagtg cggcaggctg ccgtagttgc
     1381 cgtcggcgcg gccttgcccg tcgcgctggt tgctgtgaac agggcaacgc ggacgattga
     1441 cgggaatttg gcggaagttt acgcccaaac ggtagcgttg tgcgtcggcg taattgaaca
     1501 aacgcgcttg cagcatttta tctgggctgg cgccgacacc gggaacgagg ttgctcggtg
     1561 cgaaggcgga ttgttccaca tcggcgaaga agttttcggg attgcggttc aactcgaatt
     1621 cgcccacttc aatcagcgga tagtcttttt tcggccaaac tttggtcaag tcaaacggat
     1681 gataaggtac tttttccgcg tctgcttcag gcatgacttg gatgtacatc gtccatttcg
     1741 gaaactcgcc gcgttcgatg gcttcgtata agtcgcgctg atggctttcg cggtcgtcgg
     1801 cgatgatttt ggcggcttct tcgttggtca ggtttttaat gccttgttgg gtgcggaaat
     1861 ggaatttcac ccaaaaacgc tcgcctgctt cgttccagaa gctgtaggta tgcgaaccga
     1921 agccgtgcat atggcggtag ccggcgggga tgccgcggtc gctcatcacg atggtaactt
     1981 ggtgcagtgc ttcgggcagc agcgtccaga agtcccagtt gtttgtggca gagcgcatat
     2041 tggtgcgcgg gtcgcgtttg acggctttgt tcaggtcggg gaacttacgc gggtcgcgca
     2101 ggaagaacac gggcgtgttg ttgccgacca catcccagtt gccttcttcg gtataaaatt
     2161 tcaaggcaaa accgcggatg tcgcgttctg catcggctgc gccgcgttcg cctgccacgg
     2221 tggtgaaacg ggcgaacatc tcggtttttt tgccgacttc gctgaagatt ttggcgcggg
     2281 tgtatttggt gatgtcgtgc gttacggtaa acgtaccgaa cgcgcccgaa cctttggcgt
     2341 gcatacggcg ttcggggatg acttcgcgca cgaagtcggc gagtttttca ttcagccaca
     2401 aatcctgcgc cagcagaggg ccgcgaggac cggcggtcag gctgttttga ttgtcggcaa
     2461 caggcgcgcc gttgttcatg gtcagatggg ttacagggca tttggaggta gtcatcgctc
     2521 ttgttccttt tctcaggttg gtcaaatggg ggtaaacggc ttacagtacg atttggcgga
     2581 aagcgtattc gtaaccggtt tcttgattgc aataaatttc ttgaatcgac attttatttc
     2641 ccttttgtaa aaactatgga tgcgactata cgccaagatt ttcgctatta aaactatgaa
     2701 atcgatttaa tattattata agcaatcggt tcttgatttt cgtttgtttt ttgttatcga
     2761 acggaatccg aacccgctca ttaaaaccat ttataatgca atgacgcttt gcggcatttt
     2821 ttgcgccgac aggctgaaaa taacaatttt ccccacatta tcatgacctt acttggaata
     2881 aagctcaaac agacccagca gctcaaccag cggctgcaac aatctttgcg cgtattgcag
     2941 atgtcgggta tcgaacttga acgcgaggtc gaaaactggc tgtcggacaa ccccctgctc
     3001 gaacgcaaag acccagatga attttccgat gccgggtttt cccattacac tgcgcccgcg
     3061 cgccaaatcg gcggagacga aggcgaagat atgctgtcca acatcgccgg cgagcaggat
     3121 ttcaagcaat acctgcacgc gcaagtatgc gaacacccgc tttccgacca agaagccgcc
     3181 tgtgtccaca tccttatcga tttccttgac gagcagggtt atctgaccga cagcatcgaa
     3241 gacatcctcg accatacgcc cttagagtgg atgttggatg aagcaatgct gcaacacgcg
     3301 ctgaccgcat tgaaaaaatt cgacccggca ggcgtggccg ccgccgattt gaacgaatcg
     3361 ctgatactgc agatagaaag attgggcgaa tgtgctgcca aacccgccgc cctgcatatc
     3421 gtccgaaacg ccctcgacag cattgacggc aaccgcagcc aaaccctcgc acgaataaaa
     3481 aaacacctgc cccaaaccga cagcggcaca ctcgaagccg cactcgacct cattgcttcg
     3541 ctcaatccct ttcccgccgc cggttttgcc tcgtccacgc ccacgccgta ttctgacgag
     3601 gcgctcgcca acctgctggc tttccgcggc atggaggttt ctcgccgcac cattgccaaa
     3661 tacagagaat cctttgagat tccggcagca cacaaacgca aaaccgcaga ataattgccg
     3721 aataatctta taaagacaac aaaccaaaag ccggcatttc tgcgaaagcg ggaatgccga
     3781 atccgtccgc gcggaaacct gcat
//
LOCUS       DAXHNE010000165         3720 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.165, whole genome shotgun sequence.
ACCESSION   DAXHNE010000165 DAXHNE010000000
VERSION     DAXHNE010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.165"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..217
                     /locus_tag="ACFM4Z_001852"
     CDS             <1..217
                     /locus_tag="ACFM4Z_001852"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021834.1"
                     /translation="SLHIENAAQANLQFARLVLALSHGERGLGCRLPLRFRQILTSLI
                     LRFSFAEAALSDDLLEHHRYTHLRNAV"
     gene            complement(228..551)
                     /locus_tag="ACFM4Z_001853"
     CDS             complement(228..551)
                     /locus_tag="ACFM4Z_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC6021835.1"
                     /translation="MDIVQLGPAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(631..1287)
                     /locus_tag="ACFM4Z_001854"
     CDS             complement(631..1287)
                     /locus_tag="ACFM4Z_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFC6021836.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDADPFGGFDF
                     "
     gene            complement(1390..1893)
                     /locus_tag="ACFM4Z_001855"
     CDS             complement(1390..1893)
                     /locus_tag="ACFM4Z_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFC6021837.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADEADWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGETVVVKDVNA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRF
                     EASRQAV"
     gene            2072..2845
                     /gene="tpiA"
                     /locus_tag="ACFM4Z_001856"
     CDS             2072..2845
                     /gene="tpiA"
                     /locus_tag="ACFM4Z_001856"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFC6021838.1"
                     /translation="MYRQIGMWDQKWVIGNWKMNGRLQNNNALMHRFRIHPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPNNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2852..3202
                     /gene="secG"
                     /locus_tag="ACFM4Z_001857"
     CDS             2852..3202
                     /gene="secG"
                     /locus_tag="ACFM4Z_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFC6021839.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            3216..3301
                     /locus_tag="ACFM4Z_001858"
     tRNA            3216..3301
                     /locus_tag="ACFM4Z_001858"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3250..3252,aa:Leu,seq:gag)
BASE COUNT         1013 a         1172 c          791 g          744 t
ORIGIN      
        1 cagcctgcac attgaaaatg ccgcccaagc aaaccttcag tttgcccgcc tcgtcctagc
       61 cctctcccac ggggagaggg gattggggtg caggctgcct ttaaggttca ggcaaatttt
      121 aacttcgttg atactgcgct ttagcttcgc agaagctgca ctttcagacg accttttgga
      181 acaccacagg tacacgcatt taaggaatgc cgtctgaaat gcctgcctca ataacgcatc
      241 atgttgccgt caatctcggc cgcccatgca tcgatgccgc cctgaaggtt gtacaggttt
      301 tcaaaacccg cgtccgccaa atacatcgcc gtatgcaggc tgcggatacc gtgatggcaa
      361 tacaccacaa gcggcacatc atccggcagc tcgttctgcc gcagcggaat cagattcatc
      421 gggatatgca gcgcatttgg cagcgaacaa accgccgttt cttcatccgt acgcacgtcc
      481 aacaaacaaa acatccgccc ttcgtccatc cacgctttca attccgcggg cccaagttgc
      541 acaatatcca tcgcaccccc caaaaaaaac caagcaaaat gccgtctgaa gccccaaacc
      601 cgctttcaga cggcatgacc tgtcaacatc ttaaaaatcg aaaccgccaa acggatcggc
      661 atccttatca tccaaatgcg ccaccaaggt atcgaacagc accttctctt caaacacatc
      721 gcccctgcgc gtaatcaaaa gcgcgcgttg aacaggcttg cgacctacga taaccaccat
      781 gcgtccgcca tctttcaact gttctttcaa cacttcaggc acaaggttta ccgcaccgcc
      841 gacataaacc gcatcaaacg gcgcacctgc ggaaagttcg gtcaacccgt tgttttgcac
      901 ataatcgata ttgtccaaac ccaagccgtc caacaccgct ttggcgcggt tttgctgttc
      961 ggcatcgatg tcgtccgaca ccacacgacc agccaatttt gccaacagcg cggtcgcata
     1021 gcccgaaccc gtgccgattt ccaaaaccgt atcgtttttc gtcagcttca agccctgcgc
     1081 cagccgcgcc acgactttcg gctcgagcat cttatgaccg ttggcaagcg gcagcgccat
     1141 atccgcatac gccaaaccct gcaagtcctc atcgacaaaa agctcgcgcg gaatctccgc
     1201 caaagcgtcc aacacatcaa aatccaatac atcccacgga cggatttgct gttcgaccat
     1261 attgaaccgc gctttttcaa aatccattta gtgctccgtc aaataattgt tccaacaatg
     1321 gcaggcatta taaaaccaca ctcccgccgc gccaacttcg gcacgcgccg acagcccgtt
     1381 ttgtcagtct caaaccgcct gacgcgaagc ctcaaaccgc ttctccaaaa tcttcgccag
     1441 ttcgcccaaa tacaaagcat ccgtctcatc aaactgcgcc aaatgttcgc tgtccgcgtc
     1501 caacacgccg atacagcggc cgtctgaaaa cagcggcacg acaatctccg aacgtgacaa
     1561 agacgaacag gcaatatggt cgggatgcgc gttgacatcc ttaacaacca ccgtttcacc
     1621 cttcgcccaa gcctgaccgc acaccccgcg accgaacgga atccgcgtac acgccaaagg
     1681 cccctgaaac ggtgccaaaa ccaattcgtc cgaacgcgta tcgaccaaat aaaaacccac
     1741 ccaaaaccaa ccgaacgcct ccttcaaaac cgccgccgtg ttcgccaaat tcgccaccca
     1801 atccgcctcg tcagccacca cagactcaat ctgcggcaac acctcccgat aaagcgcggc
     1861 cttgtccgaa gccgaaaaat gaagcgcgtg catcacatct cctatagttg catacatatc
     1921 aggcggccat tataaaacag cctgcccgaa acaacattcc aaaccgcccg gccggccgct
     1981 tcaagttgcg aacccgccgc atatccacta aacttcacgt tgcaccgcgc cacacgcggc
     2041 agacaaaaaa acacgacacg gagcaaaaaa gatgtatcgc caaatcggaa tgtgggatca
     2101 aaaatgggtc atcggcaact ggaaaatgaa cggccggctc caaaacaaca acgcactgat
     2161 gcaccgcttc cgcatccacc ccaccgccga acgcgtcctc atcggactcg ccgccccgac
     2221 cgtttacctg ctgcaactgc acaacgccat gcaaatcgtt ttaaacaacc gcatcctcac
     2281 ctgcgcccaa gacgtgagcc gcttccccaa taacggcgcg tacaccggcg aagtgtccgc
     2341 cgaaatgctc gccgacaccg gcacagacat cgtcctcatc ggacactccg aacgcagcct
     2401 ttatttcggc gaaaaaaacg aaatccaacg ccgcaaaatg gaaaacgtcc tcaacgtcgg
     2461 actcatcccg ttattgtgcg tcggcgaaag cctcgaagag cgcgaagccg gcaaagaaca
     2521 cgaagtcatc gcccatcagc tttccatcct gcaagggctg gataccaaaa acatcgccgt
     2581 cgcctacgaa cccgtctggg cgatcggcac cggcaaagtc gccaccgtcg aacagattgc
     2641 cgatatgcac gcattcatct acaaagaaat cttgtctttg tgcggaagcg atgttaaaat
     2701 ccgcgtcctt tacggcggaa gtgtgaaagc ggacaacgcg gccgacatct tcgcagtacc
     2761 ttatgtggac ggcgcactcg tcggcggcgc gtcattgtcg tacgactcct ttaccgccat
     2821 catcagtgcc gcacaaaatg cgtagaaaaa tatggaagcc ttcaaaaccc taatttggat
     2881 tgttaatata atttccgctt tggccgtcat cgtgttagta ttgctccaac acggcaaagg
     2941 cgcggatgcc ggcgcgactt tcggatcggg aagcggcagc gcgcaaggcg tattcggctc
     3001 tgccggcaac gctaacttcc tcagccgctc gaccgccgtt gcagcaacat ttttctttgc
     3061 aacctgcatg gctatggtgt atattcacac ccacacgaca aaacacggtt tggacttcag
     3121 caacgtacaa caaactcagc aagcacccaa acccgtaagc aataccgaac cttctgcccc
     3181 tgttcctcag cagcagaaat aacagttttt caaatgccga catggtgaaa ttggtagaca
     3241 cgctatcttg agggggtagt ggccgtaggc tgtgcgagtt caaatctcgc tgtcggcacc
     3301 aacacacaaa aacgcctgaa aatttcaggc gttttttgtt caatcccatc caacgctgtc
     3361 aaccaattcc aatcaataca aggatttcag gctgattgtt atcctgccgt cccccttcct
     3421 gacagtgcaa tcccgtccaa tccgccctaa ttgaagtaac ctaaaattta cggtatcttt
     3481 tgcggtatct gaaaaatacc tcgaaaaaat accgcaaaaa taaagctgaa cgaccgccaa
     3541 atcaggaatg ccaagcggaa aagagcttgc ggggaatact gccaagacgt agggaacaag
     3601 ggggaaaccg tccaagatgc agggcggttt tttttgggtt tttggaaaaa acctatacta
     3661 ggaagcgata cccttagttg ttaccttgtt accggggaaa agttagataa ataagcatat
//
LOCUS       DAXHNE010000166         3720 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.166, whole genome shotgun sequence.
ACCESSION   DAXHNE010000166 DAXHNE010000000
VERSION     DAXHNE010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.166"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            346..3540
                     /gene="nalP"
                     /locus_tag="ACFM4Z_001859"
     CDS             346..3540
                     /gene="nalP"
                     /locus_tag="ACFM4Z_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
                     /protein_id="HFC6021840.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGTSAPDFNAGGTG
                     IGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPNLHTGDF
                     TNPNDAYKNLINLKPAIEAGYTGRGVEVGIVDTDESVGSISFPELYGRKEHGYNENYK
                     NYTAYMRKEAPEDGDGKDIEASFDDEAVIETEAKPTDIRHVKEIGHIDVVSHIIGGRS
                     VDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFGTTSRA
                     GTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNMLFIFSA
                     SNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLSAPYEA
                     SVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIGAVGVD
                     SKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKGGSQLQ
                     LHGNNTYTGKTIIEGGSLVLYGNNKSDMRVETKGALIYNGAASGGSLNSDGIVYLADT
                     DRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAMTGGKLYMSARGKGAGYLNRT
                     GQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRGNAART
                     ASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMPGIRPY
                     GATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLDHNATG
                     LRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGHSTWSENSA
                     NAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGTLMQLG
                     ALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGLAGLKL
                     SQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVAGLGAD
                     VEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1010 a         1013 c          986 g          711 t
ORIGIN      
        1 gaaccgattc acttgttaaa gaatcgttct ctttgagcta aggcgaggca acgccgtact
       61 ggtttttgtt catccactat aactagggaa attcaaatta agttagaatt atccctataa
      121 gaaaaagccg tctaagccgg tataaacaaa ataaactcat tgagctattt gtcgaaagtt
      181 caaatttcca ttttaaaaca attagtaaaa tcgagtttat cctagttgtc caagacaacc
      241 cctataataa tataattcaa aatataaaaa tgggttacat ctaaacatta cggaattttt
      301 attccctcgc ctgaattcta ttgtcagatt caaggagacc tcatcatgcg aacgacccca
      361 accttcccta caaaaacttt caaaccggct gccatggcgt tagctgttgc aacaacactt
      421 tctgcctgct taggcggcgg cacttctgcg cccgacttca atgcaggcgg caccggtatc
      481 ggcagcaaca gcagggcaac aacagcgaaa tcagcagcag tatcttacgc cggtatcaag
      541 aacgaaatgt gcaaagacag aagcatgctc tgtgccggtc gggatgacgt tgcggttaca
      601 gacagggatg ccaaaatcaa tgcccccccg aatctgcata ccggagactt tacaaaccca
      661 aatgacgcat acaagaattt gatcaacctc aaacctgcaa ttgaagcagg ctatacagga
      721 cgcggggtag aggtaggtat cgtcgacaca gacgaatccg tcggcagcat atcctttccc
      781 gaactgtatg gcagaaaaga acacggctat aacgaaaatt acaaaaacta tacggcgtat
      841 atgcggaagg aagcgcctga agacggagac ggtaaagaca ttgaagcttc tttcgacgat
      901 gaggccgtta tagagactga agcaaagccg acggatatcc gccacgtaaa agaaatcgga
      961 cacatcgatg tggtctccca tattattggc gggcgttccg tggacggcag acctgcaggc
     1021 ggtattgcgc ccgatgcgac gctacacata atgaatacgc atgatggaac caagaacgaa
     1081 ataatgtctg cagccatccg caatgcatgg gtcaagctgg gcgaacgtgg cgtgcgcatc
     1141 gtcaataaca gttttggaac aacatcgagg gcaggcactg ccgaccattt ccaaatagcc
     1201 aattcggagg agcagtaccg ccaagcgttg ctcgcctatt ccggcggtga taaaacagac
     1261 gagggtatcc gcctgatgca acagagcgat tacggcaact tgtcctacca catccgtaat
     1321 aaaaacatgc ttttcatttt ttcggcaagc aatgacgcac aagctcagcc caacacactg
     1381 accctattgc cattttatga aaaagatgct caaaaaggca ttatcacagt cgcaggcgta
     1441 gaccgcagtg gagaaaagtt caatggctcc aaccattgcg gaattactgc catgtggtgc
     1501 ctatcggcac cctatgaagc aagcgtccgt ttcacccgta caaacccgat tcaaattgcc
     1561 ggaacatcct tttccgcacc catcgtaacc ggcacggcgg ctctgctgct gcagaaatac
     1621 ccgtggatga gcaacgacaa cctgcgtacc acgctgctga caacggctca ggacatcggt
     1681 gcagtcggcg tggacagcaa gttcggctgg ggactgctgg atgcgggtaa ggccatgaac
     1741 ggacccgcgt cctttccgtt cggcgacttt accgccgata cgaaaggtac atccgatatt
     1801 gcctactcct tccgtaacga catttcaggc acgggcggcc tgatcaaaaa aggcggcagc
     1861 caactgcaac tgcacggcaa caacacctat acgggcaaaa ccattatcga aggcggttcg
     1921 ctggtgttgt acggcaacaa caaatcggat atgcgcgtcg aaaccaaagg tgcgctgatt
     1981 tataacgggg cggcatccgg cggtagcctg aacagcgacg gcattgtcta tctggcagat
     2041 accgaccgat ccggcgcaaa cgaaaccgtg cacatcaaag gcgatctgca gctgggcggc
     2101 gaaggtacgc tgtacacacg tttgggcaaa ctgctgaaag tggacggtac ggcgatgacc
     2161 ggcggcaagc tgtacatgtc ggcacgcggc aaaggggcag gctatctcaa ccgtaccgga
     2221 caacgtgttc ccttcctgag tgccgccaaa atcgggcggg attattcttt cttcacaaac
     2281 atcgaaaccg acggcggtct gctggcttcc ctcgacagcg tcgaaaaaac agcgggcagt
     2341 gaaggcgaca cgctgtccta ttatgtccgt cgcggcaatg cggcacggac tgcttcggca
     2401 gcggcacatt ccgcgcccgc cggtctgaaa cacgccgtag aacagggcgg cagcaatctg
     2461 gaaaacctga tggtcgaact ggatgcctcc gaatcatccg caacacccga gacggttgaa
     2521 actgcggccg ccgaccgcac agatatgccg ggcatccgcc cctacggcgc aactttccgc
     2581 gcagcggcag ccgtacagca tgcgaatgcc gccgacggtg tacgcatctt caacagtctc
     2641 gccgctaccg tctatgccga cagtaccgcc gcccatgccg atatgcaggg acgccggctg
     2701 aaagccgtat cggacgggtt ggaccacaac gctacgggtc tgcgcgtcat cgcgcaaacc
     2761 caacaggacg gtggaacgtg ggaacagggc ggtgttgaag gcaaaatgcg cggcagtacc
     2821 caaaccgtcg gcattgccgc gaaaaccggc gaaaatacga cagcagccgc cacactgggc
     2881 atgggacaca gcacatggag cgaaaacagt gcaaatgcaa aaaccgacag cattagtctg
     2941 tttgcaggca tacggcacga tgcgggcgat atcggctatc tcaaaggcct gttctcctac
     3001 ggacgctaca aaaacagcat cagccgcagc accggtgcgg acgaacatgc ggaaggcagc
     3061 gtcaacggca cgctgatgca gctgggcgca ctgggcggtg tcaacgttcc gtttgccgca
     3121 acgggagatt tgacggtcga aggcggtctg cgctacgacc tgctcaaaca ggatgcattc
     3181 gccgaaaaag gcagtgcttt gggctggagc ggcaacagcc tcactgaagg cacactggtc
     3241 ggactcgcgg gtctgaagct gtcgcaaccc ttgagcgata aagccgtcct gtttgcaacg
     3301 gcgggcgtgg aacgcgacct gaacggacgc gactacacgg taacgggcgg ctttaccggc
     3361 gcgactgcag caaccggcaa gacgggggca cgcaatatgc cgcacacccg cctggttgcc
     3421 ggtctgggcg cggatgtcga attcggcaac ggctggaacg gcttggcacg ttacagctac
     3481 gccggttcca aacagtacgg caaccacagc ggacgagtcg gcgtaggcta ccggttctga
     3541 cggacaggaa gcagacagcc gcaaagatca cggtctttgc ggctgtttct tatgaaaaga
     3601 aaaccctatt ccaattgcct gcttctattg tttcaagact tcttccaaag attcggcatc
     3661 aatcagatgt atagcggatt aacaaaaatc aggacaaggc ggcgaagccg cagacagtac
//
LOCUS       DAXHNE010000167         3708 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.167, whole genome shotgun sequence.
ACCESSION   DAXHNE010000167 DAXHNE010000000
VERSION     DAXHNE010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.167"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(153..398)
                     /locus_tag="ACFM4Z_001860"
     CDS             complement(153..398)
                     /locus_tag="ACFM4Z_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021841.1"
                     /translation="MLKSIELNSHIRNRLAEYLKGRGMDFQTAMQEEKGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            510..938
                     /locus_tag="ACFM4Z_001861"
     CDS             510..938
                     /locus_tag="ACFM4Z_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HFC6021842.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            950..1426
                     /locus_tag="ACFM4Z_001862"
     CDS             950..1426
                     /locus_tag="ACFM4Z_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFC6021843.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GKQAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(1776..2264)
                     /locus_tag="ACFM4Z_001863"
     CDS             complement(1776..2264)
                     /locus_tag="ACFM4Z_001863"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFC6021844.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPAIDRTHWKEAERTERRVSSKGTRYAFVHYL
                     RY"
     gene            complement(2332..3387)
                     /gene="aroG"
                     /locus_tag="ACFM4Z_001864"
     CDS             complement(2332..3387)
                     /gene="aroG"
                     /locus_tag="ACFM4Z_001864"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFC6021845.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAEHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAS"
BASE COUNT          837 a          972 c          872 g         1027 t
ORIGIN      
        1 ccgcctgcgc gggaatgacg gctcaaaagt tacgaaacga aaaataaccg aaaccaaaca
       61 agtcggattc ccgcgcgggc gggaatgacg ataagcgtaa tttcaatctg ccatattgtc
      121 ttttcacttg cctgaaaaca gatttcagcc acctatccct gcatccggcg gctgatgtag
      181 tcggcaatca aatcccgctt ttcccaaaaa aacttctgca ttttttgttc ggaatacagt
      241 ttgcggacca gagtgggcaa accgctgtgg acgatggcgg cgatttcttt gttgcctttt
      301 tcttcctgca ttgccgtctg aaaatccata cccctgcctt tcagatattc tgcaaggcgg
      361 ttgcggatgt gggaattgag ttcgatggat ttgagcattt tgcctccgtt ttgcggtaag
      421 gatttattcg atttgtttat ccgcctgatt ttatcacgtt tcccaagcgg tttgcctgta
      481 caataccgct tttcggcagg gtgtgggcga tgatgttttc ttggttcaag ctgtttcact
      541 tgttttttgt catttcgtgg tttgcagggc tgttttacct gccgaggatt ttcgtcaata
      601 tggcgatgat tgatgtgccg cgcggcaatc ccgagtatgt gcgtctgtcg ggcatggcgg
      661 tgcggctgta ccgttttatg tcgccgttgg gcttcggcgc ggtcgtgttc ggcgcggcga
      721 taccgtttgc cgccggctgg tggggcagcg gctgggtaca cgtcaaactg tgtttgggct
      781 tgatgctctt ggcttaccag ttgtattgcg gcgtgctgct gcgccgtttt caggattaca
      841 gcaatgcttt ttcacaccgc tggtaccgcg tgttcaacga aatccccgtg ctgctgatgg
      901 ttgccgcgct gtatctggtc gtgttcaaac cgttttgacg gagcatctta tgccgataga
      961 aatcgaacgc cgttttttga ttggaaacga caactggcgg caatacgccg acgaaccgct
     1021 gctgttgagg cagggttatc tgtctgttga aaaagaacgc accgtccgtg tccgcattgc
     1081 cggaaaacag gcgtggctga cgctgaaagg ctatatttcg gaaatcagcc gcagtgagtt
     1141 tgaatacgaa atcccgcttg ccgatgcgga aaagatgatg gaaacgatgt gcccgtttaa
     1201 aatggaaaaa tggcgttatc cggtcagatg gggcggcagc ctgtttgaaa tcgatgtttt
     1261 tcttggcgat aatgcgcctt tggtcgtcgc ggaaatcgag ttgcccgacg agaatgccga
     1321 ttttgaccgt ccggactggc tgggacggga aatcactgcc gacggtatgt ttaccaatgc
     1381 ttatttgagc aggcatccgt tctcaagctg gaaaaatgcc gtctgaaacg gcttcagacg
     1441 gcattctccg acaaccatcg caaatccaaa gacagcctct atatccttat ttctgctttc
     1501 tttgttccat tattcccaat aaatccccat aacctaaaat ctcgtcattc ccgcgcaggc
     1561 gggaatccgg ttctttaaac ttcggttttt tccgataaat tgctttagct tttcgtttcc
     1621 ggattcccgc ttttgcggga atgacgatgg aagattgttg ttatttcgga taaattaccc
     1681 taactttaaa atcccggatt cccatcttcg ggggaataac aatattttct atttcagccc
     1741 atcatttgcg ggggatttta tagagagttt atatttcaat atctcaaata atgcacaaaa
     1801 gcatagcgcg tgcctttgct gctgacacgg cgttccgtcc gctctgcttc tttccaatgc
     1861 gtccggtcta ttgcggggaa aaatgcatct ccttccacag acaaatccac ttcggttatc
     1921 cgcaaatcgg tcgccaatgg catcgcttgt ccgtatatct gcgcgccgcc cataatgacg
     1981 gcttcttccg cgcctgcgca caatgccaat gccgcctcca aacttgccgc cgtttccgcg
     2041 cctgccgcgc aataatccgc ctgccggctg atgacgatgt tcctccgtcc gggcaggggt
     2101 ttgacgggca gggattccca cgttttccgc cccataatga cgggtttgcc caaggtatag
     2161 gctttgaaaa atgcgaaatc ttcggggatg tgccaaggca tagcattgcc cgccccgatg
     2221 cacaggtttt ccgcacacgc cgcaattagg gttattttga gcatatttcc tccacaaaaa
     2281 tgccgtctga acgcttcaga cgacatttta tgacattctg cgtcaaaaat ctcaactggc
     2341 gcgcgccata cgttttttgt ttgcacctgc caacaatgcc aacagttctt cagtcgcgcc
     2401 ccaaccgata cacgcatcgg taatgctctt gccgtacact tccggcttgt cctgtctgcc
     2461 ttcgaccaaa tggctttcca ccatcacgcc catgatattg ccgccgtcct gttccaattg
     2521 ggcggcaatg tcttgtgcca cttccatctg ccgagtgtaa tccttgcggc tgttggcgtg
     2581 gctgcaatct atcatcagct tgtcggttac ccctgccgca cgcagttgtt ccgccgcctc
     2641 gctgacgtgt tccgcatcat aattcggctc tttgccgccg cgcaaaatga catgacagtc
     2701 gggattgccg ccggtatgga caatggcgga atgcccggcc ttggttacag acaggaaatg
     2761 atgcgaatgg ctcgccgcac cgattgcgtc gatggcaatc ttcaaattgc cgtccgtacc
     2821 gtttttaaag ccgacggggc aggacagccc gcttgccaat tcgcggtgaa cttggctttc
     2881 ggtcgtccgc gcaccgattg ccccccaaga aatcaagtcc gcataatatt gcggcgtaat
     2941 catatccaaa aactcggtag aggcaggcat acccatattg ttcagcgaca acaacaggct
     3001 gcgcgcctga cgcaaaccga aattgatgtc aaacgtaccg tccaaatgcg ggtcgttaat
     3061 caaacctttc caacccaccg tcgtcctcgg cttctcgaaa taaacgcgca tcacaatcaa
     3121 aagctcgttt tcatactgct tgcggagttt caacaaacgc tccgcatatt ccaacgccgc
     3181 tttcggatcg tgaatcgaac acggcccgat aataaccaac agccgcttgt ccctgccgtg
     3241 aaccaaatcg gaaatttcct gacgggtgcg gtgaaccaag cccgaagcct ctttggaaat
     3301 cggcagctcg taaagatggg ctatcggcgg caacaactct ttaacttctt taatcttaat
     3361 atcgtctgtg gggtaatggt gtgtcatctt gcatctttcc tgatgggttt ttatacgcgg
     3421 cttaggttac atcgtttacc ttgcacaaac aagtgcaaca tattactaca aagctatttt
     3481 atttcaaaat aactgatata tcttttttta tagattttaa atttcatatt tttattattt
     3541 tagaaatttt atttcaaata aaaagaaaca gattgataaa aacaataccc gccattcatt
     3601 tggacaaacc tgcacggaac atccgttttc ccatttccac gacaatccgg tacagaacac
     3661 ccccgctgcc gattccgcct tcacatccgg caaaatttca tacacaag
//
LOCUS       DAXHNE010000168         3692 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.168, whole genome shotgun sequence.
ACCESSION   DAXHNE010000168 DAXHNE010000000
VERSION     DAXHNE010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.168"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            60..689
                     /locus_tag="ACFM4Z_001865"
     CDS             60..689
                     /locus_tag="ACFM4Z_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HFC6021846.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLADLGDKPYEYGKPLPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGACEAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            864..3240
                     /gene="hmbR"
                     /locus_tag="ACFM4Z_001866"
                     /pseudo
     CDS             864..3240
                     /gene="hmbR"
                     /locus_tag="ACFM4Z_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent hemoglobin receptor HmbR"
     gene            complement(3448..3570)
                     /locus_tag="ACFM4Z_001867"
                     /pseudo
     CDS             complement(3448..3570)
                     /locus_tag="ACFM4Z_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
BASE COUNT          997 a          929 c          936 g          830 t
ORIGIN      
        1 gggcaagtgc ggattttgcc cgcaccgcat ccttatccct taccgacaaa ggaaaaaata
       61 tgagtgaaac cgaaaatcaa gcattgacat ttgccaaacg cttgaaggcg gataccacgg
      121 cggttcacga cagcgtggat aacctcgtta tgtctgtcca accgtttgtc agcaaagaaa
      181 actacatcaa atttttgaaa ctccaatccg ttttccacaa ggctgtcgac cacatctata
      241 aagatgccga attaaacaaa gccattcccg agctggaata catggcgcga tacgatgccg
      301 taacgcaaga ccttgcagat ttgggtgaca aaccttacga atacggcaaa ccgctgccgc
      361 atgaaaccgg caacaaagca atcggctggc tttattgcgc cgaaggatcc aatttgggcg
      421 cggcattttt gttcaaacac gcccaaaaac tcgattacaa cggcgaacac ggcgcgcgcc
      481 acctcgcacc ccatcccgac gggcgcggca aacactggcg cgctttcgtc gagcatctga
      541 acgctttgaa cctgactccc gaagccgaag cggaagccat ccaaggcgcg tgcgaagcct
      601 ttgcattcta caaagtcgtg ttgcgcgaaa ccttcggctt ggcagccgat gccgaagcac
      661 cggaaggaat gatgccgcac aggcactaaa aaataatcga accaaataaa caaggtctcg
      721 gcatagctgt ttgcagggac ctttaattac acggcgcggc tttgtttaca tggattactg
      781 tcttattaaa tattaatgat tatcataaaa tctattattc gctaaccgat ggatgaacaa
      841 tccatacatc ttgagttgat aatatgaaac cattacaaat gctccctatc gccgggctgg
      901 tcggcagtat tttcggcaat ccggtcttgg cagcagatga agctgcaact gaaaccacac
      961 ccgttaaggc agagataaaa gcagtgcgcg ttaaaggtca gcgcaatgcg cctgcggctg
     1021 tggaacgcgt caaccttaac cgtatcaaac aagaaatgat acgcgacaat aaagacttgg
     1081 tgcgctattc caccgatgtc ggcttgagcg acagcggccg ccatcaaaaa ggctttgctg
     1141 ttcgcggcgt ggaaggcaac cgtgtcggcg tgagcataga cggtgtaaac ctgcctgatt
     1201 ctgaagaaaa ctcgctgtac gcccgttatg gcaacttcaa cagctcgcgt ttgtctatcg
     1261 accccgaact cgtgcgcaac atcgaaatcg tgaagggcgc agactctttc aataccggca
     1321 gtggtgcatt gggcggcggt gtgaattacc aaacgctgca aggccgtgat ttgctgttgg
     1381 acgacaggca attcggcgtg atgatgaaaa acggttacag cacgcgtaac cgtgaatgga
     1441 caaatactct cggtttcggt gtgagtaacg accgcgtgga tgctgctttg ctgtattcgc
     1501 aacgtcgcgg tcatgaaacc gaaagtgcgg gaaaccgagg ctatgctgtg gaaggggaag
     1561 gcagtggcgc gaatatccgt ggttcggcac gcggtatccc tgattcgtcc aaacacaaat
     1621 accacagctt tttgggtaag attgcttacc aaattaacga taaccaccgc atcggcgcat
     1681 cgcttaacgg ccagcaggga cataattaca cggttgaaga gtcttataac ctgaccgctt
     1741 cttcctggcg cgaagccgat gacgtaaaca gacggcgcaa tgccaacctc ttttacgaat
     1801 ggatgcctga ttcaaattgg ttgtcgtctt tgaaggcgga cttcgattat cagaaaacca
     1861 aagtggcggc ggttaacaac aaaggctcgt tcccgatgga ttattccacc tggacgcgca
     1921 actataatca gaaggatttg gacgaaatat acaaccgcag catggacacc cgattcaaac
     1981 gttttacttt gcgtttggac agccatccgt tgcaactcgg ggggggggcg acaccgcctg
     2041 tcgtttaaaa ctttcgtcag ccgccgtgat tttgaaaacc taaaccgcga cgattattac
     2101 ttcagcggcc gtgttgttcg aaccaccagc agtatccagc atccggtgaa aaccaccaac
     2161 tacggtttct cactgtctga ccaaattcaa tggaacgacg tgttcagtag ccgcgcaggt
     2221 atccgttacg accacaccaa aatgacgcct caggaattga atgccgagtg tcatgcttgt
     2281 gacaaaacac cacctgcagc caacacttat aaaggctgga gcggttttgt cggcttggcg
     2341 gcgcaactga atcaggcttg gcgtgtcggt tacgacatta cttccggcta ccgtgtcccc
     2401 aatgcgtccg aagtgtattt cacttacaac cacggttcgg gtaattggct gcccaatccc
     2461 aacctgaaag ccgagcgcag caccacccac accctgtctc tgcaaggccg cagcgaaaaa
     2521 ggcatgctgg atgccaacct gtatcaaagc aattaccgca atttcctgtc tgaagagcag
     2581 aagctgacca ccagcggcac tcccggctgt actgaggaaa atgcttacta cggtatatgc
     2641 agcgacccct acaaagaaaa actggattgg cagatgaaaa atatcgacaa ggccagaatc
     2701 cgcggtatcg agctgacagg ccgtctgaat gtggacaaag tagcgtcttt tgttcctgag
     2761 ggttggaaac tgttcggctc gctgggttat gcgaaaagca aactgtcggg cgacaacagc
     2821 ctgctgtcca cacagccgct gaaagtgatt gccggtatcg actatgaaag tccgagcgaa
     2881 aaatggggcg tattctcccg cctgacctat ctaggcgcga aaaaggtcaa agacgcgcaa
     2941 tacaccgttt atgaaaacaa gggctggggt acgcctttgc agaaaaaggt aaaagattac
     3001 ccgtggctga acaagtcggc ttatgtgttt gatatgtacg gcttctacaa accggctaaa
     3061 aacctgactt tgcgtgcagg cgtgtacaac ctgttcaacc gcaaatacac cacttgggat
     3121 tccctgcgcg gtttatatag ctacagcacc accaatgcgg tcgaccgcga tggcaaaggc
     3181 ttagaccgct accgcgcccc aggccgcaat tacgccgtat cgctggaatg gaagttttaa
     3241 tctggtatta ttgaattaat cgccttgttg aaaattaaag ccgtccgaat tgtgttcaag
     3301 aattcattcg gacggctttt taccgtaaat ctgtgtgatg gggttttata ttgatacagt
     3361 atccgaactc aggcaggggg taatgttgca ggattaaaaa tgccgtctga aaaatgtttc
     3421 agacggcatt ttttggttgc cgatatgtta acggacgacg ttggcgcgca tgaccacgcg
     3481 gacgtggaac gagcctgcct gacagtcgta ttgtccgcct tgcagcggct gcttgtcgaa
     3541 gtagctgtgg aagccggtaa cgccgcgtcc gcaagggtgg cggcagatgc cgcattgtgt
     3601 ttataatgcc gtgtcagacg tttgatttat gggaaaataa tgatgcttac caatcaggaa
     3661 gtaaccggtg tcaaactggg ataccgcaaa gc
//
LOCUS       DAXHNE010000169         3689 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.169, whole genome shotgun sequence.
ACCESSION   DAXHNE010000169 DAXHNE010000000
VERSION     DAXHNE010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.169"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..359)
                     /locus_tag="ACFM4Z_001868"
     CDS             complement(<1..359)
                     /locus_tag="ACFM4Z_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6021847.1"
                     /translation="MFIILGRNFLKIILCFSFFVSKFALASVNAPGKFDRVEVYDDGR
                     YLGIRGSDDKRRRIWKGVFDRESGRYLTSEAQDLKVRHVSTGASSTGKVSSVVSSSVS
                     RAGVLAGVGKLARLGAK"
     gene            complement(706..1008)
                     /locus_tag="ACFM4Z_001869"
     CDS             complement(706..1008)
                     /locus_tag="ACFM4Z_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021848.1"
                     /translation="MGYRVGINCFDTRLQADDYLLSSLPPTVTQDGKIIRPERVGDKW
                     ILNGKPVTLSYPECSNFEQIKQGSYVGSTVLILFVVIYGFRLLINFLKDIGKVGTD"
     gene            complement(1036..1266)
                     /locus_tag="ACFM4Z_001870"
     CDS             complement(1036..1266)
                     /locus_tag="ACFM4Z_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021849.1"
                     /translation="MNIVKKYAVKALLAAGIFTPAIVMADGFDASAIGTQVANVIMGF
                     VAMVSAVGMAAITVILAIQGFKMAWSMIKSVK"
     gene            complement(1504..1704)
                     /locus_tag="ACFM4Z_001871"
     CDS             complement(1504..1704)
                     /locus_tag="ACFM4Z_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021850.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEMGGGFQIFQFFVKSE"
     gene            complement(1708..2019)
                     /locus_tag="ACFM4Z_001872"
     CDS             complement(1708..2019)
                     /locus_tag="ACFM4Z_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021851.1"
                     /translation="MFNQTQTVTYPATFLGAKKFKGEIDGSNIDTCSVLVATPLPAQS
                     GNAVGFTAAQMKFGDSKNFSRLENLKYPCEVMVMVEMTSTGKGMVPSLIDFQVAEKPK
                     G"
     gene            complement(2031..3244)
                     /locus_tag="ACFM4Z_001873"
                     /pseudo
     CDS             complement(2031..3244)
                     /locus_tag="ACFM4Z_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221956.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
BASE COUNT         1169 a          864 c          545 g         1111 t
ORIGIN      
        1 aatttcgcgc ctaagcgggc aagtttgccg acccccgcca atacgccagc gcgggaaact
       61 gatgaagata caaccgaact aactttaccc gtacttgatg ctccagtaga tacatgccta
      121 acttttaaat cttgagcttc tgaagttaaa tatcttcccg attctctatc aaatacacct
      181 ttccaaattc ttcttctttt gtcatctgaa cctcgaatac ctaaatatct gccatcatca
      241 taaacttcaa ccctatcaaa tttacccgga gcatttactg atgccaatgc aaatttagat
      301 acaaaaaaac taaagcatag gataatcttt aaaaaatttc gccctaggat aataaacata
      361 acattttgct tcatctaatt taattaataa tgtagaagaa tcagggaatt gaatttctat
      421 aaaatctcta tcataacgat gaaagaaatt cctaaaaata tcaaatgaaa acgaagtttt
      481 aaaaacccct tttctaatca attccctata ataaattaac gcttgtggta aatctctata
      541 aatatgtgaa tcagtttgaa atttaactaa atgatattca gaaataaaca taattcggac
      601 cttttaaaac actctaaaaa gccgattcta ccaaccgtta ccaaaacagc caagcaacag
      661 acaaagccaa gaagaaaccg agaagaaacc agaaatctat aatcatcaat cagtcccaac
      721 cttgcctatg tcttttaaaa aattaatcag aagcctgaaa ccgtaaatga ctacaaacag
      781 aattagaacc gtcgaaccga cataagaacc ttgctttatc tgctcaaaat tggaacattc
      841 cggataagac aacgtaaccg gctttccgtt caaaatccat ttatcgccca ccctttccgg
      901 cctgatgatt tttccgtcct gggtaacagt aggaggaagg gacgacaata aatagtcgtc
      961 tgcctgcaat cttgtatcaa aacaatttat gccgacacga tagcccattt atacgcccct
     1021 ttttcttcac tctgtttatt tgacagattt gatcatgctc caagccatct tgaagccttg
     1081 gattgccaag atgaccgtaa tggccgccat acccacggcg gaaaccattg ccacaaaacc
     1141 catgattaca ttcgccactt gcgtaccaat cgcggatgca tcaaagccat ctgccataac
     1201 aatagccggt gtgaagatac cagctgccaa caatgctttt acagcatatt ttttaacgat
     1261 gttcatcgtt tttttccttt ttttgatatt taaaataaga cgacttcttg acttgcttca
     1321 tccggacgaa gtcttttccg aatctcgttt ttagccgata aaatagagga ttgcgaaaag
     1381 aaaaagaaac atacagacca cccagccgat aattagtgtt gcaaggttca ttttcatgat
     1441 atttttcctt tgttgcgggc tttgtgaaag gttaacagac cgcccgccga gcctgttttt
     1501 cttttattcc gattttacga agaactgaaa tatctggaat cctccgccca tttcatttat
     1561 gcctgaattc aacgcatctt cgtaactttc aaattgacct gctgatttaa tattttgagt
     1621 aaaccccaca tcaccgaacg gatcgggata aataaagtca tgcgtttcta aatcttgaac
     1681 tatgaaacgt tcttcaaatt tcataaatca acctttcggc ttttccgcca cctgaaaatc
     1741 aattaatgac ggaaccatgc ctttacctgt cgaagtcatt tcaaccatta ccataacttc
     1801 gcacggatat ttgagattct ctaaccttga gaaattctta ctatccccga acttcatttg
     1861 tgctgccgta aatccaacag cattacccga ctgtgccggt aaaggtgttg caaccaatac
     1921 ggaacaagtg tcaatgttag agccatcaat ttcacctttg aattttttgg ctcccaaaaa
     1981 agttgcagga taagttacag tttgagtttg attaaacatt ttgatttcct ttaaaaataa
     2041 tcataatcta cattttgatg ataaacttta cttaaatatt cctgatattt cctttctttc
     2101 tcgacatcat aatcaggact aaaaagcctt ttttccctat cgaatttgtc agaattttta
     2161 aaagcaataa cccccaattc atcaagttca atttccaaat caatatccgg ctgttcatga
     2221 ataaaaccgt gtttcaaacc gtcccttaac atttccagag catatttttc aggttctaat
     2281 cctttgggaa atcccgaatc tgcctttaaa gattccacaa tttcgctatt atcaaaaccc
     2341 atttcaatca tgaaattgac cagttttcca accgcgtttt tcgcgtaatg caatttatgc
     2401 tcgaaagtta aattaagctt tttctttctc tgatcaaacc tttcgggaac cggcatattt
     2461 ttaaattttc tacaaattgg aaaagctcca cagaaatacg aaccctgatt tattaaaata
     2521 tccaagggta tttctatatc tccataatta aactggatct cgaaccttac ccatttgctt
     2581 tctttatctc caagctgcct gcctttctca taaacacgaa caaaacgaga atttttcttg
     2641 cgacctacat aaaatgtctt gccgctcccg tcctcattcc gccaagccgt accgatcgtt
     2701 tcagatttcg gcctttgatt gctgttatca aaaaaaccat tatcgtgatc taacaacgcc
     2761 tgatccggcg tgtactctcc atcaaaaaaa tcaagtgcca aatctacccg cgttatcctc
     2821 ggcctcaatg aatcttccaa aaactgctta agcctcaatt cccaacctgg atttgcaatg
     2881 ttgcaaccta cacctttcaa ttcgattaaa accgtatttc gctgacctcc gtaatggact
     2941 tcgccgtagt caacttcttc cgatcccaac ctaaacatcg aatcgtaaaa tttattgccc
     3001 ttgatttgca tttgcgcgtt atgccaaaac ctagaatttc ttccagcttt ctgcttaata
     3061 catacatgta ttcagcatca gaaaataaag ggcaaccgga aactttcagt aaagtatctt
     3121 catggaatgt gaatgaaatc caatcaacaa aaaccccgtc ttgcttacct cttcgctgcg
     3181 gaatttctaa aagcttccct tttccgtccg atataaagtg tgagaaatat tcaacttcac
     3241 tcattcgttc actactcttg gaattggttt ttaatttcgc tccccccctg ttagtcaagg
     3301 ggggggcttt cagccgtttc ccgtcttccg cgctaaagcg cgtacgacgg tcaacgaccg
     3361 aaagccaaat cctgacaaac ttttaaagat caacaaaaaa gaccacaacc atccgttgtg
     3421 ataatcactg gaaaattcga accaaccgaa tctatataat cgaacgcctg ataaagcttt
     3481 gaaaaattct cttgattaat gagtttatgc ggttcgccat gtctgaactg ataaaaacac
     3541 aaaacacaag aatctgattt tttaaatatt ctccaataag aacaggaaaa tattacattt
     3601 actgctgaca taaaaaagcc cctttcactt ggctgtcaaa ggggaatgtt aagaaaatta
     3661 atgcgcccct ttgatagagc gcatcatat
//
LOCUS       DAXHNE010000170         3672 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.170, whole genome shotgun sequence.
ACCESSION   DAXHNE010000170 DAXHNE010000000
VERSION     DAXHNE010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.170"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..329
                     /locus_tag="ACFM4Z_001874"
     CDS             <1..329
                     /locus_tag="ACFM4Z_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HFC6021852.1"
                     /translation="TEEGIAQTVVKGKTTRAEVEARFGRQNPFGCYAYHEVSLPIYNF
                     LPTNFIYIKSERRHWEWCVDYDEEGVVRDYRFTHKVGKDERSVIRDTVDVIRKEAGKS
                     LSQPEK"
     gene            393..1676
                     /gene="hemL"
                     /locus_tag="ACFM4Z_001875"
     CDS             393..1676
                     /gene="hemL"
                     /locus_tag="ACFM4Z_001875"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFC6021853.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVIEAVREAALGGLSFGAPTEGEIAIAEQIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEA
                     FVKALRGLTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTALTSRLADG
                     IAAAAKAHGIEFTADSVGGMFGLYFASHTPRNYADMARSNIDAFKRFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELINETVAVAVEVFKAMAE"
     gene            complement(1892..2164)
                     /locus_tag="ACFM4Z_001876"
     CDS             complement(1892..2164)
                     /locus_tag="ACFM4Z_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taxon MazF"
                     /protein_id="HFC6021854.1"
                     /translation="MPSEAQILHFRRHFYSRLKSKIYTPVIPAQAGIRKVGLVGHEVV
                     LKMKRYRKSDELKEKYSDIDETFEFIKGFIYVIALAFTAYVAIRFF"
     gene            complement(2175..2738)
                     /gene="orn"
                     /locus_tag="ACFM4Z_001877"
     CDS             complement(2175..2738)
                     /gene="orn"
                     /locus_tag="ACFM4Z_001877"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFC6021855.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAIHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPERATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(2756..3643)
                     /gene="prmA"
                     /locus_tag="ACFM4Z_001878"
     CDS             complement(2756..3643)
                     /gene="prmA"
                     /locus_tag="ACFM4Z_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFC6021856.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQIAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQIEELSGIYSQWF
                     DLDPAETEEGWARLSGTKR"
BASE COUNT          771 a          962 c         1047 g          892 t
ORIGIN      
        1 agacggaaga gggaatcgca caaacggttg ttaaaggcaa aacgacccgg gcggaggtag
       61 aggcacgttt cgggcggcaa aatccgttcg gttgttatgc ctatcatgaa gtcagcctgc
      121 ctatttataa ttttttaccg accaatttca tttatataaa atcagaacgc cgacattggg
      181 agtggtgtgt ggattacgac gaggagggag ttgtccggga ctaccgcttt acacataaag
      241 tgggaaaaga cgagcgttcc gtcatccggg atacggttga cgtcatccgc aaagaagcgg
      301 gcaaatcctt gtcgcaacct gaaaaatgat aaaatggggc tttctgcttc caagcccgaa
      361 acctgccgtt cagacggcat ttgaggataa atatgaaccg taacgaaatt ttattcgacc
      421 gcgccaaagc catcatcccc ggcggcgtga attcccccgt ccgcgcattc ggcagcgtcg
      481 gcggtgtgcc gcgctttatc aaaaaagccg aaggcgcata tgtttgggac gaaaacggca
      541 cgcgctacac cgattacgtc ggctcttggg gtcctgcgat tgtcggacac gcacaccccg
      601 aagtcatcga agccgtgcgc gaagctgcct tgggcggttt gtcgttcggc gcgcccaccg
      661 agggcgaaat cgccattgcc gaacaaatcg ccaaaatcat gccgtccgtc gaacggctgc
      721 gcctcgtcag ctccggtacc gaagcgacga tgaccgccat ccgtttggca cgcggtttta
      781 ccggtcgcga caaaatcatc aagtttgaag gctgctacca cgggcattcc gacagcctgc
      841 tggtgaaagc cggcagcggc ctgctgacgt tcggcaatcc ttcttccgcc ggtgtgcctg
      901 ccgactttac caaacacact ttagtccttg agtacaacaa catcgcccaa ttggaagaag
      961 cctttgctca aagcggcaat gacattgcct gtgtgatttt ggagcctttt gtcggcaata
     1021 tgaaccttgt ccgcccgacc gaggcctttg tcaaagcctt gcgcggattg accgaaaaac
     1081 acggcgcggt gttgatttac gacgaagtga tgaccggctt ccgcgtcgcg ctcggcggcg
     1141 cgcaatcgct gcacggcatc acgcccgacc tgaccacgat gggcaaagtc atcggcggcg
     1201 gtatgccgct tgccgcgttc ggcggacgca aagacatcat ggaatgtatt tccccgctgg
     1261 gcggcgtgta tcaggcaggc acattatcag gcaacccgat tgccgtcgcc gccggcttga
     1321 aaacgctgga aatcatccgg cgcgaaggct tctatgaaaa cctgactgcc ctgaccagcc
     1381 gcctcgccga cggtattgcc gccgccgcca aagcgcacgg tatcgaattc accgccgaca
     1441 gcgtgggcgg tatgttcggt ctgtattttg ccagccatac gccgcgaaac tatgccgata
     1501 tggcgcgatc caatatcgac gctttcaaac gcttcttcca cggcatgctc gaccgcggca
     1561 ttgcctttgg cccgtccgct tatgaagcag gtttcgtttc cgccgcgcat acgcccgagc
     1621 tgattaacga aacggttgcg gttgcggttg aagtgttcaa ggcgatggct gaatgatgtt
     1681 ttgacggaca gagtttctct gttcgatttg tttggcagat tgaagtaaga atgcacaccg
     1741 ccgtcattcc cgcgcaggcg ggaatccaga cctttcagtt tctgtaatga ttgaaaataa
     1801 cgtgccgaaa tcagccgcac ccggcaggaa gccgccgcca cctgaaacaa tgccgtctga
     1861 agccttcaga cggcatttta tcggaagcgc atcagaaaaa acggatagcg acatatgcgg
     1921 taaaagcaag ggcaattaca tagataaacc cctttataaa ttcaaaagtc tcatctatat
     1981 cgctatattt ttctttcagt tcgtcagatt ttcgataacg tttcattttc aagaccactt
     2041 catgaccaac aagcccgacc ttccggattc ccgcctgcgc gggaatgacg ggcgtgtata
     2101 tttttgattt caatctgctg taaaaatgcc gtctgaaatg caggatttgc gcttcagacg
     2161 gcattgtttg tttcttattg cgcttcggct ctcggggcgg aaatcagaaa gtgttcgcgg
     2221 tagtggcgca tttcttcgat gctctccaaa atgtcgtcca atgccttgtg cgaaccgcgt
     2281 ttgaccacgc ttttggcaac gggcggattc cagcgtttgg caagctcttt gagcgtggaa
     2341 acgtcgaggt tgcggtagtg gaagtagttt tccagtttcg gcatatattt gaccataaaa
     2401 cgccggtctt ggtggatgga gttgccgcac atcggcgtgg cgcgttcggg tacccattcc
     2461 gacataaagt ccagcagttt ctgttcgact tcggcttcgg tatgcgacga ttcgcgtacg
     2521 cgctgcgtca gccccgtcct accgtgtgtg gcggtgttcc actcgtccat attgtcgagc
     2581 agctcgtcgc tttggtggat ggcgtaaact tcggattgcg ccaatacatt caaatccgag
     2641 tcggtaataa tcatcgcgac ttcgataatg cggtcggttt cgggattaag ccccgtcatt
     2701 tccatatcga gccagcatag gttgttttta tcctgcatga tgtttccttt gcttgtcagc
     2761 gttttgtccc gcttaatcgc gcccatcctt cttcggtttc cgcaggatcg agatcgaacc
     2821 attggctgta aatgccgctg agttcttcga tttgttcgtc caacaaaccg gataacacga
     2881 tgcggccgcc ttgtttggtg cgggcggcaa gcatttcgcc gagcatacgt aaagggttgg
     2941 cgaggatgtt ggcaacaact acgtcgaatt gcccttgagg caggccgtcg ggcaggaaga
     3001 attgtgcatc gacgttgttc tgttcggcgt tgtccttgcc ggcgcggacg gcttgttcgt
     3061 caatatcgac gccgacggca gaacctgcgc cgagtttgag ggcagcgatg gtcaggatgc
     3121 ccgaaccgca gccgtagtcg aggacgcttt cgccgttttt gagttgcgta tccagccatt
     3181 tgaggcagag gcgcgtggtt gggtggctgc cagtgccgaa ggcgaggccg ggatcgaggc
     3241 gtaggttgac ggcagtgcct tcgggggctt cgtgccaaga gggggtaatc cacaggcggt
     3301 cggaaattcg gatggggtcg aattgcgatt gcgtgagacg cacccagtct tggtcttcga
     3361 tggtttcgcc ggtgtatgcc aagtctttta acccgcattc ttgtgcggcg atttggatga
     3421 tggcggcggc ttcgtcgtgt tcgccgaaca gggcgatgac tttgctctgc tgccagattt
     3481 gttcggcggg cataccgggt tcgccgaaaa tcgcctgttc gttttgcgtg ccggcgtagg
     3541 catcttcgat ggcggcggag agtgcgccgt gttccatcag cgcgtcggcg aggcgttcgg
     3601 cgacggcatc gttgacgttg acggtgattt gttggtaggg cataacgggc tttcttggaa
     3661 atatagtgga tt
//
LOCUS       DAXHNE010000171         3566 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.171, whole genome shotgun sequence.
ACCESSION   DAXHNE010000171 DAXHNE010000000
VERSION     DAXHNE010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.171"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(122..976)
                     /gene="tsf"
                     /locus_tag="ACFM4Z_001879"
     CDS             complement(122..976)
                     /gene="tsf"
                     /locus_tag="ACFM4Z_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFC6021857.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(1105..1833)
                     /gene="rpsB"
                     /locus_tag="ACFM4Z_001880"
     CDS             complement(1105..1833)
                     /gene="rpsB"
                     /locus_tag="ACFM4Z_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFC6021858.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(2010..2243)
                     /locus_tag="ACFM4Z_001881"
     CDS             complement(2010..2243)
                     /locus_tag="ACFM4Z_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021859.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWAVLPTVFTVFV
                     VTVRRRNRLMKQQEQAEFEQQRAQRQKDSGTKP"
     gene            2321..2902
                     /locus_tag="ACFM4Z_001882"
     CDS             2321..2902
                     /locus_tag="ACFM4Z_001882"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFC6021860.1"
                     /translation="MRNEEKHALRRELRGRRSQMGWDVRAAATVKINRLLKRYIKHGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     AKLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVGTLPREAHDLPLDGFVSEAGILCF"
     gene            2963..3553
                     /locus_tag="ACFM4Z_001883"
     CDS             2963..3553
                     /locus_tag="ACFM4Z_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFC6021861.1"
                     /translation="MSERIILPVLSLGGVRLEPLDVHHEMGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT          760 a          835 c         1047 g          924 t
ORIGIN      
        1 tgttttgttt tcagacggcc tttatattcg gtcgtcttaa aataggtacc gtaaacggtt
       61 ttctcaaaaa aaagcaccct gattcgtttg gaatccaggt gctttctttt tcataagtgc
      121 cttacacttt ggcggctgcg gctacttcgg ctgcgtagtc gacaacggct ttctcgatac
      181 cgtcgcctac tttgtagcgg acgaagctga ccacttcagt gccgttttct ttggcgaatt
      241 gggcaacagt ttgatcgggg ttcatcacga atgcttggcc gttcagagtg atttcagcca
      301 agaatttgcg gatacggcct tcaaccattt tagcggcgat gtcggcaggt ttgccggaag
      361 cgatggcttg ttcggtgtag atgtggcgtt ctttttcaac ggtttcggca tctacttcgg
      421 cttcgcttac gcattgtggt ttagcggcaa caatgtgcat accgattttg cgtgctacgt
      481 cttcagagcc tttgtactca accaatacgc cttcggtcgc caatgcgccg tggatgtagg
      541 caaccagttg gttggcagtg tcgatcactt ggaagcggcg gacagacatg ttctcgccca
      601 atttggcgat gatggctttg cgttctgctt caaccagttc gctcagttct tcaacagaag
      661 ccggtttttt ctcggcagca gttttcgcaa cgaagttggc aaattctacg aagcccgcgt
      721 ctttagcaac gaagtcggtt tcgcagttta cttcgaccaa tgcgccgaca ttgccgttga
      781 tcgcgtaagc caatacgcct tcggcagcgg tacggccggc cagtttaccg gctttcgcac
      841 cggatttgat acgcaggatt tcttcggctt tgtcgaagtt gccttcggct tcaaccaagg
      901 cttttttgca ttccatcatg cccaggccgg tagcggcgcg caggtcggca accatttttg
      961 cagtaatttc tgccattttg aatctcctag atttttggga aatacggaaa ggattttccg
     1021 cattcgggaa tcgtgtgccg cgaacggacg ttcagacggc atatttgaga aaaggggcat
     1081 aacgcccctc ttcggtttgc cggattactc ggcagcggct tcttgggcag cggctacggt
     1141 ttcttgcagc gcttggtttt tgccttccaa aactgcgtca gcgatgccgc ggcagtacag
     1201 gcggatggct ttggcggagt cgtcgttgcc ggggatgacg tatttcacgc cgtcagggct
     1261 gttgttggta tcgactacgg cgataacggg gatgcccagt ttttcagcct cgaccagagt
     1321 acctttttgg tagccggtat cgataacgaa aatcgcgtcg ggcaggcctt tcatgttttt
     1381 gataccgccc aaagaacgtt ccagtttttc aacatcgcgt tgcatttcca gaatttcttt
     1441 tttgctgaaa ccgctttcgg cagcattttc caaggctgcg gttttttctt ccaggcgttt
     1501 gatggattgc ttaacggttt tgtagttggt cagcataccg cccaaccagc ggtaatcgac
     1561 gaaaggcata ccggcgcggg tcgcttcttc gcggatgatg tcgcgtgcct ggcgtttggt
     1621 acctacgaac aatactgtac ctttgttggc aaccaaacga cgtacggctt cttgcgcgtc
     1681 ttggaacatc ggcagggttt tttccaggtt gacgatatgg attttgttgc gcgcaccgaa
     1741 aatgtattga gccattttcg ggttccagaa acgggtttgg tggccgaagt gaacgccggc
     1801 ttcaatcatc tgacgcatag taatttgaga cattttgatt tccttgaaag ggttaagggc
     1861 acacattcaa tcgcatagaa cttgataggc gatgcctatt gtcaagcacc cttcggcgaa
     1921 agtgggcata agttttaaaa aataaaagag tgtttccgaa atggaaaact gacggattat
     1981 agcggataag aggccgtctg aaaagggatt cagggttttg tgccgctgtc tttttgccgt
     2041 tgtgcacgct gctgttcgaa ttctgcctgt tcctgttgct tcatcaagcg gttgcggcgg
     2101 cggacggtta cgacgaagac cgtgaacacg gtgggcagca ccgcccaaaa aaccaaataa
     2161 atcaaggcgc gcgcaatact cggctgcgcg gcggaataca tcacggcaac aaaaagatag
     2221 ccgatggcga caatgtgcca cattgtgtgc gtcctgtttg aatgttaaca aaatcgttat
     2281 aatcgcaaca ttctaccgca atcctgaaag gccgtctgaa atgaggaacg aggaaaaaca
     2341 cgccctgcgc cgcgaattac gcgggcggcg ttcgcaaatg gggtgggacg tgcgggcggc
     2401 ggcaacggta aaaatcaacc gcctgctcaa acgttatatc aagcacggtc ggaaaatcgg
     2461 cgtgtattgg ccgatgggca aggaattgcg tttggacggc tttgtccgcg cggcgcaaaa
     2521 acgcggcgca aaactttatc tgccttatat cgaaccgcgt tcgcggcgga tgtggtttac
     2581 gccgtatcct gaaagcggaa tggaacggga gcgcatacgg ggcagggcga agttgaacgt
     2641 gccgcagttt gcagggcgca aaatccgcgt gcacggtttg tcggtattgc tcgtcccgct
     2701 tgtcggcata gaccgcgaag gctaccgctt ggggcaggca ggcggctatt acgatgcgac
     2761 gcttgcagcg atgaaatacc gtttgcaggc aaaaaccgtg ggcgtgggct ttgcctgcca
     2821 actggtcggc acgctgccgc gcgaggcgca cgacctgccg ctggacggtt ttgtgtcgga
     2881 ggcggggata ttgtgttttt agccgttcag acggcattcc gggcggaaat gccgtctgaa
     2941 ggcggaatat tgggagaaaa ggatgtcgga aaggataata ctgccggtct tgtcgttggg
     3001 cggcgttcgt ctcgaaccgt tggatgtgca tcatgaaatg ggtttgcgcg aggcggtttg
     3061 cgatggggaa gtttggaagc tgggagtaac gtccgcgccc catccggata gggtggcaga
     3121 ctatatcggg acagcattgg caacgcgttt ggcgtttgcg gttgtcgatg aagaggcggg
     3181 cagggtggtc gggacaacgg cgtattatca ttttgaaccg cagattccgc gtttggatat
     3241 cggattcaca tggtatgcgg catcggcacg gcggacacgg ataaacacct gttgcaaaat
     3301 catgctgttg gattacgcat ttgatgtttt ggcttgccgt tgcgtgggat ggcgcacgga
     3361 tattctgaac cttgcctcgc aacgtgccat cgaacggctg ggtgcggaaa aagacggcgt
     3421 gctgcgtatg catatgctcc gtaaagacgg cagtgtgcgc gatacggtcg tgtacagtat
     3481 gctgagggaa gattggtgca aaaacaggga aatcctgacc ggcaggttgg cagggtatgg
     3541 cgtgcaggtg tgaggcagta ttttca
//
LOCUS       DAXHNE010000172         3505 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.172, whole genome shotgun sequence.
ACCESSION   DAXHNE010000172 DAXHNE010000000
VERSION     DAXHNE010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.172"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(152..385)
                     /locus_tag="ACFM4Z_001884"
     CDS             complement(152..385)
                     /locus_tag="ACFM4Z_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021862.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPQKRESVFLSFSHFR"
     gene            complement(415..3180)
                     /gene="tdfH"
                     /locus_tag="ACFM4Z_001885"
     CDS             complement(415..3180)
                     /gene="tdfH"
                     /locus_tag="ACFM4Z_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc piracy receptor TdfH"
                     /protein_id="HFC6021863.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHYSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQDGQLKFNSDSGKWER
                     DLQRQQWKYPWYKKYENPQELQKYIEEHDKSWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNSYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYSSEGEFKRAFGENSPTYKKHCNPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTRDVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADGLCNGKYGGTSKSVLTNFARGRTFL
                     ITMSYKF"
BASE COUNT          817 a          855 c          870 g          963 t
ORIGIN      
        1 agaaatttaa tgttgcggca ctaaccaaaa aaaccgaaac cgaacggtct agattcccgc
       61 ctgcgcggga atgacgaatc catccgtacg gaaacctgca ccacgtcatt ctcacgaaag
      121 tgggaatccg gaaagctaaa gccacaggaa tttatcggaa atggctgaaa cttaaaaaaa
      181 ctgattcccg cttttgcggg aatgacgact cataaattac ccgaaacaac caaaaataac
      241 cgaaatcaaa aaccggcttc cctgcgtggg aatgaaatgg atttttcttt gagttgccga
      301 tgcgataaaa cggattggca taggcttgcg gcaggatgat gcgtttgggg aaacaaatgc
      361 cgtctgaagg gctttcagac ggcattgcgg ttttccacaa aacgcgggct gcctttaaaa
      421 cttgtagctc atcgttatca aaaaggtgcg tccgcgtgca aaattggtca atacgctttt
      481 gcttgtgccg ccgtatttgc cgttgcacaa gccatcagca ttacacgtta cgtcttcgtc
      541 cttgtctttc gggtcgaacg agctgtaata acgctgcgtt gccgcatcat tgcccgcatc
      601 gagcggatcg atataacgcc tgtcgaacag atttttgact tcggcgcgga aaataaggtt
      661 tttcttcggc tcgtaagcgg cgtaaaaatc aaaaatcaaa ggctggcggg caagggtttc
      721 ggtttgtttg atggaacgct tgcccagttg ccggacatcg cgggtatttc ccccgttggt
      781 gccgtcgata tagcgttctt cagccgtcgc gcggatgctc ttgccgaaat agcgcatcgc
      841 gccgcccaaa gtcagcttgt tgcccaacca gcgcgtaccg acttccaaac gtccgtaatc
      901 tcgcggcagg gcggaaaccc tgctcaaccc ataaccttgt ttgagttggt cttctttgga
      961 cgcattgttg ggcgattcgc tcgcatcgct gaagttggtc ggttgcgtgc ttttttgata
     1021 ggcgtaagaa aggttggtga aaaaacgccc ataatcgtaa ttcagctcca actcaaaacc
     1081 gtgtttgtgc actttgtctt tgaaattgcg atgttggatg gtgtaggcaa gcccggtgct
     1141 gctgacccag ctcggaatat ccccgttcaa atcccaccat ttcccgtaaa cgttgtggat
     1201 gtagttgtcg atgcggctgc ggtagccgac cagttttaat cctaatgtat catcttgttt
     1261 taacagtcct tttttatagg tattgaagcc aaattgccaa gtgtttgcgc gctctggttt
     1321 taaggcggtg tgaacgccgg agtcgccgat ttgggaaaaa tacatttctt ggatgttggg
     1381 catacggtgt gtgcgcgaat agctggcgaa cggcatgaaa taatcgccga agtccgcact
     1441 aatgctgacc gaatggttgt tggcgcgctt tttgccgtat tttttcaata cgggttcata
     1501 aagcccgcag ctcgggttgc aatgtttctt gtatgtcggc gagttttctc cgaatgcccg
     1561 cttaaattcg ccttccgagc tgtaatagcc cgtatattcg ccgccgaaac ggtagccgac
     1621 ggtattggtg ctgtagttta agcggtaaat gtcttttttg agcgcggcat cgaagtagaa
     1681 cgtgttgaaa tattggctgc cggccggttg gacaatggtt gatttttggg gcagcagccc
     1741 tttatcgccc ttaaaccgcc ccaaatagga ataaagcccg ttgtcctgat caggaccgtc
     1801 gaaaaacagc cccaattctt caggaaagcg gtttttgccg tattcgttgt ggaaataatt
     1861 gaagcccaaa gtggtttgca actcggtttc gcggggcagc cggaaggtgg cggtgttgtt
     1921 gaggtcgagg atttttgcgt tgttgtaggt ttcaaaatcc tttaaaagcc cccagcctgt
     1981 aaacttcgac cctttcggat atttctgcct gcccgaattg taggctgcgg tcagattgag
     2041 gttggtatac gagttcaaag acaaaccgta attgaattga taattgcggt tgatgatttt
     2101 gcggctgccg attttggtgt ttaaatcgcg aaattgcgcc gtgtatttat tgaatacgcc
     2161 gtcgtattcc aatttaaaca gattacctgc cgactgctgc ttcaggctgg acggatcgat
     2221 gggggtgatg tcgtattgcg gcgccaggtt ttcccgccag cttttgtcat gctcttcgat
     2281 gtatttttgc agttcttggg ggttttcgta ttttttatac cacgggtatt tccactgttg
     2341 tctttgcaag tcccgctccc attttccgct gtcggaattg aatttcaact ggccgtcttg
     2401 tacaaaatat cgttgcttgc gccgttccag atattccgcg ccaaaatttc cgatgtgctg
     2461 cccgccgccg cccacgcggt aattttgcgc cacgctgcgc ctgctgtgcc cgtaaagcac
     2521 accgacagat gctccgcttt ccagccattt gcgcgcacct atcgccgcca tcgcattacc
     2581 tttggttgaa ttggtgccgg tcagaccttt tagcagcagg ccgtaggtat tattgccctg
     2641 aacgacgtca tccacgccta aagtccgcag attcgccgaa ccggcaaggc tgttgatgcc
     2701 tgccgagccg ctgaagctgc ctttgacgac atccagtccg gcaataaaat tgctgtcgac
     2761 agatgcaccg aattgagatg aaccgcctgc cctgcccgca tcggtagaag tcgaataaaa
     2821 ggtctgcgtg atgccgtcca ccatcgtatt gacccgcccg aacccgctgt cgccgcgaat
     2881 attcaaagac acaatgcccg agcttttatc ttgctgtgta aacgcaccgg ggatgctgcg
     2941 tacgatgttg tcgaggtttt cgctggattt gaatatatcc tgacgggtcg atacggcacg
     3001 cgcatcggta aacacttttt tgtctttcgg tacgcgcttc gccttgacgt gcacatcttc
     3061 caaaacctgt atctgcgcct cgctgcccgc gcgccctgca tcttcggcat aactataatg
     3121 atatagcata acacccataa ggtaaaaaca aatcggcttc aaccggaaag aagatctcat
     3181 attttcctca acaataaaca gtcagacaat taggaaatat actagcattt ttttcaggcg
     3241 gcataaacat taaaaaagtg taaatttgat ataccgtctg aagatttcaa ttggatattt
     3301 atagtggatt aacaaaaacc agtacagcgt tgtctcgctt tagctcaaag agaacgattc
     3361 tctaaggtgc tgaagcacca agtgaatcgg ttccgtactg tttgtactgt ctgtggctcg
     3421 ccgccttgtc ctgattttta ttaatccgct ataaagaccg tcgggcatct gcagccgtca
     3481 ttcccgcgca ggcgggaatc tagtc
//
LOCUS       DAXHNE010000173         3472 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.173, whole genome shotgun sequence.
ACCESSION   DAXHNE010000173 DAXHNE010000000
VERSION     DAXHNE010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.173"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            100..1215
                     /gene="lst"
                     /locus_tag="ACFM4Z_001886"
     CDS             100..1215
                     /gene="lst"
                     /locus_tag="ACFM4Z_001886"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HFC6021864.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAEW
                     AYFFHLPYGLNKSFNFIPTMAELKVKAMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTINLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKD"
     gene            complement(1379..1837)
                     /locus_tag="ACFM4Z_001887"
     CDS             complement(1379..1837)
                     /locus_tag="ACFM4Z_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6021865.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PEFTRMGKMVKDEEPYDVEKFKQAAASFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            1956..2777
                     /locus_tag="ACFM4Z_001888"
     CDS             1956..2777
                     /locus_tag="ACFM4Z_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6021866.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDERAAGVYEGVEFIRPEDIAETALW
                     LYQRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            complement(2828..3274)
                     /gene="yciA"
                     /locus_tag="ACFM4Z_001889"
     CDS             complement(2828..3274)
                     /gene="yciA"
                     /locus_tag="ACFM4Z_001889"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFC6021867.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVDVWVKTL
                     MNDCVTEDRYLVTEAVFTYVAIDAEGNPRPIPKEGNPKLAGLLPTP"
BASE COUNT          825 a          744 c         1001 g          902 t
ORIGIN      
        1 tattcggtaa ctgtcggaat atctgctaaa attccgcatt tttccgtccc gggacactcg
       61 gggcgtatgt tcaatttgtc ggaatggagt tttagggata tgggcttgaa aaaggcttgt
      121 ttgaccgtgt tgtgcctgat tgttttttgt ttcgggatat tttatacatt tgaccgggta
      181 aatcaggggg aaaggaatgc ggtttccctg ctgaaggaca aactcttcaa tgaagagggg
      241 gaaccggtca atctgatttt ctgctatacc atattgcaga tgaaggtggc agaaaggatt
      301 atggcgcagc atccggggga gcggttttat gtggtgctga tgtctgaaaa caggaatgaa
      361 aaatacgatt attatttcaa tcagataaag gataaggcgg agtgggcgta ttttttccac
      421 ctgccctacg gtttgaacaa atcgtttaat ttcattccga cgatggcgga gctgaaggtg
      481 aaggcaatgc tgctgccgaa ggtcaagcgg atttatttgg caagtttgga aaaagtcagc
      541 attgccgcct ttttgagcac ttacccggat gcggaaatca aaacctttga cgacgggaca
      601 atcaatttaa ttcaaagcag cagctatttg ggtgatgagt tttctgtaaa cgggacgatc
      661 aagcggaatt ttgcccggat gatgatcgga gattggagca tcgccaaaac ccgtaatgct
      721 tccgacgagc attacacgat attcaagggt ttgaaaaaca ttatggacga cggtcgccgc
      781 aagatgactt acctgccgct gttcgatgcg tccgaactga aggcggggga cgaaacgggc
      841 ggcacggtgc ggatactttt gggttcgcct gacaaggaga tgaaggaaat ttcggaaaag
      901 gcggcaaaaa acttcaacat acaatatgtc gcaccgcacc cccgccaaac ctacgggctt
      961 tccggcgtaa ccacattaaa ttcgccctat gtcatcgaag actatatttt gcgcgaaatt
     1021 aagaaaaacc cgcatacgag gtatgaaatt tatacctttt tcagcggtgc ggcgttgacg
     1081 atgaaggatt ttcccaatgt gcacgtttac gcattgaaac cggcttccct tccggaagat
     1141 tattggctca agccggtgta tgccctgttt acccaatccg gcatcccgat tttgacattt
     1201 gacgataaag attaatcgca tagcaaatca aaatagaaaa tggcggagta agtaaggcaa
     1261 aaatcaggat atggcgtatt ttttgaattg aagataattt ttgattgctt tgcgcttggt
     1321 gaaatggcaa agaaaatgcc gtctgaaggc ttcagacggc attgttctgt ttcggatgtt
     1381 attcgggtgc gcggaaactg tcgtggcagg atttgcagct tgcgccggtt tcgccgtagg
     1441 cggctttgat ttcatccagt ttgccggttt gtgctgcggc gttgagtttt tcgacggcgg
     1501 cggcgaattt tgttttttcg gcttcaaatt ttgcaccatc cgaccaaacg gcgggcaggg
     1561 cgcggccgtt gccttgcgga tcggactcaa aaagtgtgaa cggtttcttg ctgctttcgg
     1621 caaacgacgc tgccgcctgt ttgaattttt cgacatcgta aggttcttcg tctttgacca
     1681 ttttgcccat gcgtgtgaat tcgggcatca tggatttgaa cgcggcggtg cggttttcgg
     1741 aaatttcgcc tttgggttgg gaaggtattc cgctgcctcc gcaggcggaa aggagcagtg
     1801 tgatggcggc agcagcaagg ctgatttggg ttttcatatt gaatgtgtcc tgtcgtggtg
     1861 gtatggttgt tgtcattttc agtcggcgcg atgccgtctg aagggtgtta tgatacctga
     1921 aacaggtttg ggaaacgaga aagaaaggaa aaacaatggc tgttttaatt accggtgctt
     1981 cggcaggatt cggcgaagcg atgtgccgtg cgtttgtcgg ggcgggatac cgcgttatcg
     2041 gtgcggcgcg ccgtgcggac aggcttcagg ccttggcgga tgaattgggt gctttgtttt
     2101 accctttgga aatggacgtg tcgcgacgcg agtcggtgga aaacgcctta aacggcatcc
     2161 ccgatgaatt ttccgacatc gactgcctca tcaacaatgc cgggctggct ttgggtctgg
     2221 acacggcgga caaggcggat tttgaagatt gggaaacgat gattcaaacc aatgttttgg
     2281 gtttgacgtt cctgacgcgc aaaattttgc cgcaaatggt ggaacgcggc ggcggttatg
     2341 tgatgaattt gggttcgatt gcaggcaatt atgcttatgc cggcagcaac gtttacgggg
     2401 cgaccaaggc gtttgtgcgc cagttcagcc tgaatttgcg cgcggagttg gcggataaga
     2461 acatccgcgt taccaatatc gagccgggtt tgtgcggcaa tacggagttt tccaatgtgc
     2521 gcttcaaagg cgatgacgag agggcggcgg gcgtgtatga gggtgtggaa tttatccgcc
     2581 ccgaagatat tgcggaaacc gcattgtggc tgtaccagcg gccggcgcat atgaatgtga
     2641 acacgattga aattatgccc gtggcgcaga cttttgcagg aatgaaagtg ataaaaaaag
     2701 ccgtgcccga agtgcgggaa gactttgaaa aacagagtat gtcgctgttt tcccgcatca
     2761 ggtcctggtt caaatgatgc aaaatgccgt ctgaagacag tttcagacgg catttttacg
     2821 ggtattttta cggagtaggc aataagcccg ccaatttggg gttgccttct ttcggaatcg
     2881 ggcgcggatt gccttccgca tcgatggcaa cataagtgaa cacggcttcg gttacgaggt
     2941 agcggtcttc ggtaacgcaa tcgttcatca aagttttcac ccagacgtcg actttaagct
     3001 ggagggaagt gttgcccacg cggacgcaat gcccgtagca gcagacgacg ttgccgacct
     3061 tgaccgggcg gatgaagttc atttcctgaa cggcgacggt aacgatgcgt ccccgcgcga
     3121 tttccgccgc caatatgccg ccgcccaaat ccatctgcga cataatccag ccgccgaaaa
     3181 tgtcttggtt gggattggta tcgcgcggca tagcgacggt acgcaggagc agttcgcctt
     3241 gagggcgttg gcggttgcct tcttcgtgct gcataaagtt tccttgtttt attgaaatat
     3301 aaatcgaacc tgcacccctg cccgaaacgg gaagtcggga agtgtgtagt ttaacccatt
     3361 tgagacatac aagggcgggc gcggaacaga ttcgcaaggc gtattgtagg gcggggctgt
     3421 agagtgggct tcagtccgcc aatcccgcca aatcctaccc taagcaactg aa
//
LOCUS       DAXHNE010000174         3461 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.174, whole genome shotgun sequence.
ACCESSION   DAXHNE010000174 DAXHNE010000000
VERSION     DAXHNE010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.174"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            170..679
                     /locus_tag="ACFM4Z_001890"
     CDS             170..679
                     /locus_tag="ACFM4Z_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021868.1"
                     /translation="MDILLKYWKPVGVLLLIVLIFTAWHFDRAEKYRMGREAAAAEIS
                     NRLKDGYIEQAKQARSAEQKAAAAFAERQTKLEEEKQNAEKTVAAMRLELNRLRHYAA
                     AQNRSRNLPATTTAATASDGASDSQGWLLFGQCAEKYAGLAETADGHAADLREWQAYG
                     HAISSQRAE"
     gene            complement(732..1079)
                     /locus_tag="ACFM4Z_001891"
     CDS             complement(732..1079)
                     /locus_tag="ACFM4Z_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC6021869.1"
                     /translation="MYIPDKGDIFHLNFDPSSGKEIKGGRFALALSPKAFNRATGLVF
                     ACPISQGNAAAARSSGMISTLLGAGTETQGNVHCHQLKSLDWQIRKASFKETVPDYVL
                     DDVLARIGAVLFD"
     gene            complement(1081..1317)
                     /locus_tag="ACFM4Z_001892"
     CDS             complement(1081..1317)
                     /locus_tag="ACFM4Z_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFC6021870.1"
                     /translation="MLRVQKWGNSAAVRLPADMLKQLDFKIGDALVAEVHNGELRVRA
                     ARRFRLADLLAEMEETPPRVEGWEILDDVGNEVV"
     gene            complement(1676..2167)
                     /locus_tag="ACFM4Z_001893"
     CDS             complement(1676..2167)
                     /locus_tag="ACFM4Z_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HFC6021871.1"
                     /translation="MFWQLTVVSVTAVIALGTIFINKKTSKQKATLDVILNDYQDAQF
                     VEADNHISPYIRGTAVDDNNARIDLYEIYQNKGGQWEKERGHLLTVINRHEFYACAIN
                     SGVLDEDLFKRLHCTNFIKLWNAVSPLVMKIREEERKDTIFRELEILVALWKANPLKA
                     SDL"
     gene            complement(2419..2589)
                     /locus_tag="ACFM4Z_001894"
     CDS             complement(2419..2589)
                     /locus_tag="ACFM4Z_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021872.1"
                     /translation="MPSEIQTAFLLKGFPSTALHKGGFFMPSESLSDGIGLHGRSPPA
                     FKQERMSRMPDF"
     gene            complement(2605..2973)
                     /locus_tag="ACFM4Z_001895"
     CDS             complement(2605..2973)
                     /locus_tag="ACFM4Z_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFC6021873.1"
                     /translation="MIDGELVALIHQTVLADEAGLKGRADMARLDGALSQIANWRQYE
                     NLEDIYEIAALYAQAIAKAHAFPDGNKRTALLTMLTYLDLQGISIAADQGLDDLIVSL
                     AAGETDFKQLAETLRRLDKE"
     gene            complement(2970..3101)
                     /locus_tag="ACFM4Z_001896"
     CDS             complement(2970..3101)
                     /locus_tag="ACFM4Z_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021874.1"
                     /translation="MQNQNTRPVKIELKGEAGKRVLLAAARRIAKTHQKAVKALADK"
BASE COUNT          870 a          852 c          790 g          949 t
ORIGIN      
        1 cgctgaaatt cgaccagttg atactcgaat ttcctgagcg cggggacggc gcatgggtac
       61 acatcggttt ccgacgcaac agcccgcaac gcaaccagat actgaccgca accaagaaaa
      121 acggcaaaac cgtgtatctg cccgggctgc atccttgagg tcgtctgaaa tggatatttt
      181 attgaaatac tggaagccgg taggtgtatt gctgctaatc gtcctgattt ttaccgcatg
      241 gcatttcgac cgtgccgaaa aataccgcat gggacgggag gctgctgctg ccgaaatctc
      301 gaatcgtctg aaagacggct atatcgagca ggcaaagcag gcgcgttctg ccgagcagaa
      361 ggccgctgcc gcgtttgccg aacgacaaac caaattagaa gaggaaaaac aaaatgctga
      421 aaaaactgtt gccgctatgc gtcttgagct taaccgcctg cgccactacg ccgccgccca
      481 aaatcgcagc agaaacctgc ccgcaaccac taccgccgcc accgcatctg atggcgcgtc
      541 agattcccaa ggctggctat tattcggaca gtgcgctgaa aaatatgcag gactggcaga
      601 aacagccgac ggacatgcgg ccgatttaag ggaatggcag gcatatggtc atgcaataag
      661 cagtcagcgt gccgaataag caaccgccgg aacctgtaat cttttcttac aggttcgggc
      721 ggtttcggca tttaatcgaa taagacggcg ccgatgcgcg ccagcacatc gtccaataca
      781 taatcgggta cagtttcttt aaaagaagcc ttgcggattt gccagtccag agatttgagc
      841 tggtggcagt ggacattgcc ctgcgtttcc gttcctgcac cgagtaaggt tgaaatcatg
      901 ccgctgcttc gtgcagccgc tgcattcccc tgtgaaatgg ggcaggcaaa aaccaatccc
      961 gttgcgcggt tgaatgcttt tggagacaga gccagcgcaa accgcccgcc cttgatttcc
     1021 ttgccgctgg aagggtcgaa attcaaatgg aaaatatcgc ctttgtcggg aatatacatt
     1081 tcagacgacc tcgttgccga catcatccaa gatttcccag ccttctacgc gcggcggggt
     1141 ttcttccatt tcggcaagca agtctgccaa gcggaaacgt cgggcagcac gcacacggag
     1201 ttcgccgtta tgtacttccg ctaccaaagc gtcgccgatt ttaaaatcca attgtttcag
     1261 catgtcggca ggcagtcgga cggccgccga gttcccccat ttttggacac gcaacataat
     1321 cttcaccttt attgtatcta caaagtagat acatattacc ataaaatttc agttgttcaa
     1381 atacttgtgc agaatacgcc aaaagccgtc cgaactgttt cggacggctt ttgtactgta
     1441 tttgcgcctt caggcaatat tttgttatcc attttcaaga tgcaaaagct ttctaattgc
     1501 ttgatgtcgg atttcggttg tttagggata caaaaccaag taaactaaaa ctgtttgatt
     1561 ggaaaatgct ccgcaaggag aaaattatgt tcaaaaaatc actttataag gctgctttgg
     1621 cgtatttcgg cgattgcgtg gctgcccata tttcagaaca gttttgactg tttattcaca
     1681 aatcagatgc ctttaggggg tttgctttcc ataatgcaac caaaatttcc aactctctaa
     1741 atattgtgtc tttgcgttct tcttcgcgta ttttcataac aagaggcgaa actgcattcc
     1801 acaattttat gaagttggtg caatgcagcc gtttaaacaa atcctcatcc aatactcccg
     1861 agttgattgc gcacgcataa aactcgtgcc gattgattac ggtaagtaaa tgccctctct
     1921 ctttttccca ttgtccgccc ttattttgat aaatttcata caggtcgatc cgcgcgttgt
     1981 tgtcgtcaac tgccgtgccg cgaatataag gcgaaatatg attgtcggct tctacaaatt
     2041 gtgcatcttg gtaatcattc aaaataacat ctaatgtcgc cttttgcttt gaagttttct
     2101 tattgatgaa tattgtcccc agtgcaatga cggcggttac tgaaacaacg gtcagttgcc
     2161 aaaacatcag ccattcggcc gtccctcata ggttaaactg tacgctgtgc ctgaatatgt
     2221 aaaataaagt gaatacagat acaaacaagg aggttagggc aattactgac aaccaacctc
     2281 cttgttttat ttgtttccgc accttaccag ccattaaaac cctcattttt gtatttcatc
     2341 gtatttcctt aatggtttta accttgcttg tcgatatctg tatattaatg ccgtgcgccc
     2401 gttctttcaa ggggtgattt aaaaatcagg catccttgac atcctctcct gcttaaaggc
     2461 ggggggactc ctgccgtgta aaccaatgcc gtctgaaagg ctttcagacg gcataaaaaa
     2521 accgcctttg tgtaaagcgg ttgaaggaaa gcctttcaat aaaaatgccg tctgaatttc
     2581 agacggcatt gttgtcggat atgcctattc cttatccagc cggcgcaggg tttcggcgag
     2641 ctgtttgaag tcggtttctc ccgccgccaa actgacaatc aagtcgtcaa gcccttggtc
     2701 ggcggcaatg ctgatgccct gcaaatccag ataggtcaac attgttaaaa gcgcggtgcg
     2761 cttgttgccg tcgggaaagg cgtgggcttt ggctatggct tgtgcataga gggcggcgat
     2821 ttcgtagatg tcctcaaggt tttcatactg ccgccagttg gcaatctgcg acaatgcgcc
     2881 gtccaagcgc gccatatccg cccgcccttt caaacccgct tcatccgcca atacggtttg
     2941 atggataagc gcgaccagtt cgccgtctat catttgtcgg caagtgcctt gacggctttt
     3001 tgatgggttt tggcaatgcg gcgggcggcg gcaagcagta cgcgtttgcc tgcttcgcct
     3061 ttaagctcga ttttgacggg gcgtgtattt tggttttgca tcgctgctcc ttgtctgttt
     3121 gcgggcattt tagctttttt ccggcagctt gggaaatgcc gtccgaaaac acttcagacg
     3181 gcattcttct aatagtgtaa tgcaatagtt actccaagat ttttgtatat ggctgttttt
     3241 atggtaaatt aaattttcat aagaaataaa tatatttaaa tcaatgaaat aaaatttagt
     3301 cgaatcccac cgtttccgcc acaaaacaaa accgccctga ttcggggcgg ttcttttttg
     3361 ttcaagttga gacctttgca aaattccttt ccttcccgac agccgaaacc caaacacagg
     3421 ttttcagcta tttccgcccc aaatacctcc taattttacc c
//
LOCUS       DAXHNE010000175         3439 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.175, whole genome shotgun sequence.
ACCESSION   DAXHNE010000175 DAXHNE010000000
VERSION     DAXHNE010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.175"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            43..708
                     /locus_tag="ACFM4Z_001897"
     CDS             43..708
                     /locus_tag="ACFM4Z_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HFC6021875.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKDGTANNKCDSGKKEQGMLAFGDKNGNK
                     EYNDTADVLLRSVVLNDDINNKRINYAFNHIAFGHTQPTADRVVWTFNQNGTFGYTKD
                     RHLTKQSSFFYSDGYIQIVLTDARAVSDADKKFRSAVVLIDSSGRVEVCPRNDRRTVC
                     QYK"
     gene            738..1352
                     /gene="pilV"
                     /locus_tag="ACFM4Z_001898"
     CDS             738..1352
                     /gene="pilV"
                     /locus_tag="ACFM4Z_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HFC6021876.1"
                     /translation="MKNNDCFRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYIGNHHTLSAVDGDFAVDA
                     VKTKTQLAEAQLKRFSYELKNALPDAAAINYAVCKDSSGVAPTLSAGSTFSSNCDGSA
                     NGDTLIKVLWVNDSAGDSDIARTNLETNGNNIVYTYQARVGGRE"
     gene            1349..2338
                     /locus_tag="ACFM4Z_001899"
     CDS             1349..2338
                     /locus_tag="ACFM4Z_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HFC6021877.1"
                     /translation="MRRKMLNVPKGNYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLSAQQDLRNAATLIVRDARMAGGFGCFNMSEHTKNDIIVDPSKQTQH
                     VPVKPGAKQENPLFSLEWANTNNNTAKLIPIAESTDIKYPGFAQARPALIFQYGIDDL
                     DASAETVVVSSCSKIAKPGKKISTLQEAKSALQITNDDKQNGNITRQRHVVNAYAVGR
                     IADEEGLFRFQLDDKGKWGNPQLLAKKIRHMKVRYIYVSDCPEDDDAGKEEKFKYMGT
                     FDSSTNAVTPAGVEVLLSSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            2317..2910
                     /locus_tag="ACFM4Z_001900"
     CDS             2317..2910
                     /locus_tag="ACFM4Z_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HFC6021878.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCGKGLCTAVNVRT
                     NNDNEEAFDNIVVQGKPTVEAVKRSCTAKSTGLCIDNKGMEYKKGTQSVSKMPRYIIE
                     YLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            2915..3388
                     /locus_tag="ACFM4Z_001901"
     CDS             2915..3388
                     /locus_tag="ACFM4Z_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX family type IV pilin"
                     /protein_id="HFC6021879.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNISKQFILKNPLDDNQTIKSKLEIFVSGYKMNPKIAEKYNVSVHFVNKEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDAASARAHLETLSSDVGCGAFSNRKK"
BASE COUNT          988 a          689 c          859 g          903 t
ORIGIN      
        1 aatttaatcc actatacatt accagttaac gtcctattgc ttatgtgtac acgaaaacaa
       61 caaggtttca cgctaacaga gctgctcatc gtgatggtca ttgcagccat tatggcgatg
      121 atagccctcc ccaatatgag ccaatggatt gcatcccgcc gcattgccag tcatgcggag
      181 cggattgcca accttttgcg tttctccagg ggcgaagccg tccggctcaa tctccctgtc
      241 tatatctgtc ctgttcaagt taaaaaagac ggtacggcca acaataaatg tgactccggc
      301 aagaaggagc agggaatgtt ggctttcggc gacaaaaacg gcaataagga atataatgat
      361 acggcggatg ttcttctccg cagtgtggta ttgaatgatg atatcaataa taagcggatt
      421 aattatgcct tcaaccatat cgctttcggt cacacgcaac cgaccgccga ccgcgttgtt
      481 tggacgttca atcaaaacgg gacgttcggt tatacgaaag accggcatct tacaaaacaa
      541 tccagctttt tttactccga cggttatatc caaatcgtgc tgacagatgc gagagcagtt
      601 tcagatgccg ataagaaatt ccgttcggcg gtggttttga ttgacagcag cggcagggtt
      661 gaggtttgtc ctagaaacga taggcgtacc gtatgccaat ataaatgatt gttttagtcg
      721 gtttgggaaa taaaaaaatg aagaataatg attgcttccg cctgaaaaac ccccagtccg
      781 gtatggcgct gatagaagtc ttggtcgcta tgctcgttct gaccatcggt attttggcac
      841 tattgtctgt tcagttgcgg acagtcgctt ccgtcaggga ggcagagacg caaaccatcg
      901 tcagtcaaat cacgcaaaac ctgatggagg gaatgttgat gaatccgacc attgattcgg
      961 acagcaacaa gaaaaactat aatctttaca taggaaacca tcatacacta tcagctgtgg
     1021 atggcgattt tgcggttgat gccgtgaaaa ctaagacgca gttggcagag gcacaattga
     1081 agagatttag ttatgagctg aaaaatgcct tgccggatgc ggcagccatc aattacgccg
     1141 tctgcaagga ttcgtcgggt gttgcgccga cattgtccgc cggcagtact ttttcttcaa
     1201 attgcgatgg tagtgcaaat ggggatactt tgattaaagt attgtgggta aatgattcgg
     1261 caggggattc ggatatcgcc cgtacgaatc ttgagacgaa cggcaacaat atcgtatata
     1321 cctatcaggc aagggtcgga ggtcgggaat gagacgtaaa atgctaaacg taccaaaagg
     1381 caattatgat ggtatgaagg gttttaccat tattgaattt ttggttgcgg gcatgctcag
     1441 tatgattgtc ctgatggcgg tcggatcgag ttacttcaca tcccggaaat taaatgatgc
     1501 ggcaaacgag cgtctttccg cgcaacagga tttgcggaat gcggcaacat tgattgtccg
     1561 cgatgcaaga atggcagggg gcttcggttg tttcaatatg tccgagcata ctaaaaatga
     1621 tattattgtt gatccaagta agcaaactca acatgtccct gtaaaacccg gtgccaaaca
     1681 agaaaatccc cttttttctt tagagtgggc taatactaat aataatacag ctaaattgat
     1741 tcctattgct gaatccacag atattaaata tccgggtttt gcccaggctc gtccggcatt
     1801 gattttccaa tacggcatcg atgatcttga tgcgagtgct gagactgttg tagtcagcag
     1861 ctgttccaaa atagcaaaac cgggtaagaa aatatctacc ttgcaagaag caaagagtgc
     1921 attacagatt actaatgatg ataaacaaaa tggaaatatc acccgtcaaa ggcatgtggt
     1981 caatgcctat gcggtcggca ggattgccga tgaggaaggt ttgttccgct tccaattgga
     2041 tgataagggc aagtggggta atcctcagtt gctcgcgaaa aagattagac atatgaaagt
     2101 gcggtatatc tatgtttccg actgtcctga agatgacgat gccggcaaag aggaaaaatt
     2161 caaatatatg ggtacattcg acagctccac aaatgctgtt acgcccgccg gggtggaggt
     2221 tttattgagt agcggtactg ataccaagat tgccgcttct tcagacaatc atatttatgc
     2281 ttaccgtatc gatgcgacaa tacgcggggg aaatgtatgc gcaaacagaa cactttgacg
     2341 ggaatcccga cttctgacgg acagaggggg tttgcactgt ttatcgtgct gatggtgatg
     2401 atcgtcgtgg cttttttggt tgtaactgcc gcgcagtctt acaataccga gcagcggatc
     2461 agtgccaacg aatcagacag gaaattggct ttgtctttgg ccgaggcggc tttgcgggaa
     2521 ggcgaacttc aggttttgga tttggaatat gatacggaca gtaaggttac atttagcgaa
     2581 aactgtggaa aaggtctgtg taccgcagtg aatgtgcgga caaataatga taatgaagag
     2641 gcttttgaca atatcgtggt gcaaggcaag cccaccgttg aggcggtgaa gcgttcttgc
     2701 actgcaaaat ctacaggcct gtgcattgac aataaaggga tggaatataa gaaaggcacg
     2761 caaagcgtca gcaaaatgcc acgttatatt atcgaatatt tgggcgtgaa gaacggagaa
     2821 aatgtttatc gggttactgc caaggcttgg ggtaagaatg ccaataccgt ggtcgtcctt
     2881 caatcttatg taagcaataa tgatgagtaa taaaatggaa caaaaagggt ttacattgat
     2941 tgagatgatg atagtcgtcg cgatactcgg cattatcagc gtcattgcca taccttctta
     3001 tcaaagttat attgaaaaag gctatcagtc ccagctttat acggagatgg tcggtatcaa
     3061 caatatttcc aaacagttta ttttgaaaaa tcccctggac gataatcaga ccatcaagag
     3121 caaactggaa atatttgtct caggctataa gatgaatccg aaaattgccg aaaaatataa
     3181 tgtttcggtg cattttgtca ataaggaaaa accaagggca tacagcttgg tcggcgttcc
     3241 aaagacgggg acgggttata ctttgtcggt atggatgaac agcgtgggcg acggatacaa
     3301 atgccgtgat gccgcttctg cccgagccca tttggagacc ttgtcctcag atgtcggctg
     3361 tggagccttc tctaatcgta aaaaataggg ctgttttgcc gatgccgtct gaaaatcaat
     3421 gttcagacgg catttttat
//
LOCUS       DAXHNE010000176         3435 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.176, whole genome shotgun sequence.
ACCESSION   DAXHNE010000176 DAXHNE010000000
VERSION     DAXHNE010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.176"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     regulatory      65..168
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            235..1458
                     /gene="cytX"
                     /locus_tag="ACFM4Z_001902"
     CDS             235..1458
                     /gene="cytX"
                     /locus_tag="ACFM4Z_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HFC6021880.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRQARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIESFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            1455..2555
                     /locus_tag="ACFM4Z_001903"
     CDS             1455..2555
                     /locus_tag="ACFM4Z_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFC6021881.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIVLFDKGCRRGENAAAYV
                     AAAMLAPAAEAVEATPEVIRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADEIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPEDLQTQYDWLIDCRGYGAKTAWNQAPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYNRARRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            2577..3194
                     /gene="thiE"
                     /locus_tag="ACFM4Z_001904"
     CDS             2577..3194
                     /gene="thiE"
                     /locus_tag="ACFM4Z_001904"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFC6021882.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGDE
                     LKREIARCVAACQGSRTQLFINDHWHEAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELNRALSVHPSYVASGAIFPTTTKQMPTAPQGLDKLCEYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT          706 a         1069 c          947 g          713 t
ORIGIN      
        1 aatgcgccgt gccgccgaaa atgtaagaat tttgccaacg cacgttgctt tgtgtaaact
       61 taaagctttc ttgtcggagt gccgccgccg ggcggctgag attgcgaaag cagaatccgt
      121 agaacctgtc ggggtaatgc ctgcgtagga aacaaaccgt caaatgcctt atcaggcttc
      181 cgttcccttt tccgcacttc cccgcccacc ttccttcttt ttaaaggacg tcatatgtcg
      241 ggcaatgcct cctctccttc atcttccgcc gccatcgggc tgatttggtt cggcgcggcg
      301 gtatcgattg ccgaaatcag cacgggtacg ctgcttgcgc ctttgggctg gcaacgcggt
      361 ctggcggctc tacttttggg tcatgccgtc ggcggcgcgc tgttttttgc ggcggcgtat
      421 atcggcgcac tgaccggacg cagctcgatg gaaagcgtgc gcctgtcgtt cggcaaacgc
      481 ggttcagtgc tgttttccgt ggcgaatatg ctgcaactgg ccggctggac ggcggtgatg
      541 atttacgccg gcgcaacggt cagctccgct ttgggcaaag tgttgtggga cggcgaatct
      601 tttgtctggt gggcattggc aaacggcgcg ctgattgtgc tgtggctggt tttcggcgca
      661 cgcaaaacag gcgggctgaa aaccgtttcg atgctgctga tgctgttggc cgttctgtgg
      721 ctgagtgccg aagtcttttc cacggcaggc agcaccgccg cacaggtttc agacggcatg
      781 agtttcggaa cggcagtcga gctgtccgcc gtgatgccgc tttcctggct gccgctggcc
      841 gccgactaca cgcgccaagc acgccgcccg tttgcggcaa ccctgacggc aacgctcgcc
      901 tacacgctga ccggctgctg gatgtatgcc ttgggtttgg cagcggcgtt gttcaccgga
      961 gaaaccgacg tggcaaaaat cctgctgggc gcaggtttgg gtgcggcagg cattttggcg
     1021 gtcgtcctct ccaccgttac caccactttt ctcgatgcct attccgccgg cgcgagtgcg
     1081 aacaacattt ccgcgcgttt tgcggaaaca cccgtcgctg tcggcgttac cctgatcggc
     1141 acggtgcttg ccgtcatgct gcccgttacc gaatatgaaa acttcctgct gcttatcggc
     1201 tcggtatttg cgccgatggc ggcggttttg attgccgact ttttcgtctt gaaacggcgt
     1261 gaggagattg aaagctttga ctttgccgga ctggttctat ggctggcggg cttcatcctc
     1321 taccgcttcc tgctctcgtc cggctgggaa agcagcatcg gtctgaccgc ccccgtaatg
     1381 tctgccgttg ccattgccac cgtatcggta cgccttttct ttaaaaaaac ccaatcttta
     1441 caaaggaacc cgtcatgacc cgtatcgcca tcctcggcgg cggcctctcg ggaaggctga
     1501 ccgcactgca gcttgcagaa caaggttatc agattgtact tttcgataaa ggctgccgcc
     1561 ggggcgaaaa cgccgccgct tatgttgccg ccgcgatgct cgcgcctgcg gcggaagcgg
     1621 tcgaagccac gcctgaagtc atcaggctgg gcaggcagag cattccgctt tggcgcggca
     1681 tccgatgccg tctgaacacg cacacgatga tgcaggaaaa cggcagcctg attgtgtggc
     1741 acgggcagga caagccatta tccagcgagt tcgtccgcca tctcaaacgc ggcggcgtgg
     1801 cggatgacga aatcgtccgt tggcgcgcag atgaaatcgc cgaacgcgaa ccgcaactcg
     1861 gcggacgttt ttcagacggc atctacctgc cgaccgaagg ccagctcgac gggcggcaaa
     1921 tattgtctgc acttgccgac gctttggacg aactgaacgt cccttgccat tgggaacacg
     1981 aatgtgcccc cgaagacctg caaacccaat acgactggct gatcgactgc cgcggctacg
     2041 gcgcaaaaac cgcttggaac caagcccccg agcacaccag caccttgcgc ggcatacgcg
     2101 gcgaagtggc gcgggtttac acacccgaaa tcacgctcaa ccgccccgtg cgtctgctcc
     2161 atccgcgtta tccgctctac atcgccccga aagaaaacca cgtcttcgtc atcggcgcga
     2221 cccaaatcga aagcgaaagc caagcccccg ccagcgtgcg ttcagggttg gaactcttgt
     2281 ccgcactcta tgccgtccac cccgccttcg gcgaagccga catcctcgaa atcgccaccg
     2341 gcctgcgccc cacgctcaac caccacaacc ccgaaatccg ttacaaccgc gcccgacgcc
     2401 tgattgaaat caacggcctt ttccgccacg gtttcatgat ctcccccgcc gtaaccgccg
     2461 ccgccgtcag attggcagtg gcactgtttg acggaaaaga cgcgcccgaa cgcgatgaag
     2521 aaagcggttt ggcgtatatc cgaagacaag attaaagccg cccgaaagga caccttatga
     2581 ccttcccgcc cctaaaatcc ccgctcaaat tctacgccgt cgtccccacc gccgattggg
     2641 tggggcgcat ggtcaaagca ggtgccgaca cggtgcaact gcgctgcaag accctgcacg
     2701 gcgatgaatt gaaacgcgaa atcgcccgct gcgtcgccgc ctgtcaaggc agccgcacac
     2761 agcttttcat caacgaccac tggcacgagg caatcgaagc gggcgcatac ggcgtacatc
     2821 tcgggcagga agacatggac accgccgacc ttgccgccat cgccgccgcc ggtttgcgtt
     2881 taggtttgag tacgcactcc gtcgccgaac tcaaccgcgc cctgtccgta catcccagct
     2941 acgtcgccag cggcgcgatt ttcccgacca cgaccaaaca aatgcccacc gccccgcaag
     3001 gcttggacaa actgtgcgag tatgtgaaac aggcaggcgg cacgcccgtc gtcgccatcg
     3061 gcggtatcga cttgaacaac gcccgagccg tactcgccac cggcgtttcc tcactcgccg
     3121 ccgtccgcgc cgtaaccgaa gcggcaaatc ccgaagcggt ggttaaagcg tttcaggctt
     3181 tgtgggatgg ataaaaccga aagaagaaaa ttcaattgcc gtgtaggcaa aacttagccc
     3241 gttatcgcaa acatacttaa ctttaaatgt ggcatatcat caaattccgt cattcccgcg
     3301 caagcgggaa tccgccttaa aacttgagaa accatcattt gaaaaacagt ttccgaattt
     3361 caaaaatgga ttcccgcctg cgtgggaatg acggcaaccg gccggttgcg tatcaaaaaa
     3421 taaagtaatt cggct
//
LOCUS       DAXHNE010000177         3397 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.177, whole genome shotgun sequence.
ACCESSION   DAXHNE010000177 DAXHNE010000000
VERSION     DAXHNE010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.177"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(223..861)
                     /locus_tag="ACFM4Z_001905"
     CDS             complement(223..861)
                     /locus_tag="ACFM4Z_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFC6021883.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            1116..1958
                     /locus_tag="ACFM4Z_001906"
     CDS             1116..1958
                     /locus_tag="ACFM4Z_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFC6021884.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPEPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPSNASAKADSEQGNGE
                     DKGTGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2024..2686
                     /gene="exbB"
                     /locus_tag="ACFM4Z_001907"
     CDS             2024..2686
                     /gene="exbB"
                     /locus_tag="ACFM4Z_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HFC6021885.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            2689..3123
                     /locus_tag="ACFM4Z_001908"
     CDS             2689..3123
                     /locus_tag="ACFM4Z_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HFC6021886.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
BASE COUNT          897 a          793 c          954 g          753 t
ORIGIN      
        1 acggcaataa atccagcatt tgattggcaa gttttggcga tgtgaaaacc tcatcattag
       61 acagactgga gtttcttaat cagtgtttca gttaatgaaa ggtattatca tgcaacttgg
      121 ttctaataat gatgaaaaaa ataagattac actacaggaa aacgcggcag ggcaatatgt
      181 cctgccgcgt tttctggagc gtttatgccg tctgaaagcc agtcagacga acatctgcac
      241 aaacgcaatc accagccgca caatcggtgc aataaacgca cccaaaaccc cggtcagcat
      301 cagcagtagg ataatccacg tcccataagg ttcgattttg cggaacgctt gcgaatattt
      361 cgccgacagg aaggtgtcga tgaaaatgcc gccgtcccaa ggcaggatgg ggatgatgtt
      421 gagcgcgaac agaatcgcat tgatcagaat accgtagttt gccatttgag ccaacggcat
      481 ctgatacgcc ccgccgacat acggagtcag caccaaaacc acgccccaca gaacagccat
      541 cgccagattc gacagcgggc cggacgcggc aacgcaacgc caggcaaggc gcgggttgcg
      601 gaagttgcgc gaatcgatag gaatcggacg cgcccagccg aacaggaagg gcgtgaacat
      661 caaagtaagc agcggtacga tgattgtgcc gaccaaatcg atatggggca gggggttcag
      721 tgtcagcctg ccgtattgtt cggcagtgtt gtctccccag tagcgcgccg tatagccgcg
      781 cgccacctcc ctgacggtaa tggcgagcag cacgggcagg acggcaagca gaaacacgcc
      841 caaatcaaaa ttttgaaaca tggttttcct ttgaacagat gtggtgtgca gcccgaaacg
      901 gctgacagac agaagcggca atatatcatc taacctgatt ttattggaat aagaaatcag
      961 acggaatcat tttttctgcc ggcccgttat ctgatgattt gctgtaatac agcgtttaaa
     1021 cacggtttgc ctgtgaacgg aacagacatt catatgacca aaaattgtaa aaataggttg
     1081 caaataggaa ttgttgctat tattatgccc gaattatgga taaagaacga attttaaccc
     1141 ccgcagtcgt gttttccgtc gcactgctgc atttggcaat ggtggcattg ctctggcagg
     1201 cgcacaagct gcccgtgata gagtcaggaa atgttatcga atttgtcgat ttgggtgatt
     1261 ttggcggagg ggacggtgca cccgaaggcg caggtgcgcc tgctgcgcct gagccgcaac
     1321 ctgagcctga gccgcccaaa cctgtcgagc cgcccaagcc ggttttgaag ccggtggtta
     1381 cgaaaaaggc ggatgcggat attcagcagc ctaaggaaga gccgaaacct gaagaaaagc
     1441 cgaaacccga agaaaaaccg aaaccagagc ctaagccgga agcgaagcct gtcccgaaac
     1501 cggcggaaaa accggtcgag aagccgtctg aaaaacctgc cgaacatccc agcaatgctt
     1561 ctgccaaagc agacagcgag cagggtaatg gggaagataa gggaaccggt accaaaggag
     1621 acggaacggg gcgcggagaa ggcagcggta aaggtagcgg cggtgtcaaa ggcgaacacg
     1681 gggaaggagc cggcagcagc aaaggcaatc ctttacgcgc caacggcagc attccgcgcc
     1741 cggcttatcc cacgctttct atggagaatg acgagcaggg tacggttgtt ttgagcgttt
     1801 tggtttctcc gggcggtcat gtcgagtccg ttaaaatcgt gaaaagcagt ggtttttctc
     1861 ggttggacaa tgcggcacgc aaggcggcgc aaaacgggca ttttcaagcc aatgcctgga
     1921 cggagtttaa agtccccgtc aagtttgaat tgaattaggc ggttgccgca gcggtttgcg
     1981 gcagcttgga tgtttgattg gttttttcag tgttggaatc attatgaatt tgaaattagt
     2041 atttgaatcg ggcgatcccg tcctgatcgg tgtgtttgtg ttgatgctgt tgatgagtat
     2101 cgtaacgtgg tgtttggttg tcttgcgctg catcaagctg tatcgggcgc gcaaagggaa
     2161 tgccgccgtc aaacggcata tgcgcgatac tttgtcgctg aacgacgcgg tcgaaaaagt
     2221 gcgcgccgtc gatgcgcctt tgtccaaact ggcgcaagag gcattgcagt cttaccgcaa
     2281 ctaccgccga aacgaagcgt ccgaactggc gcaggctttg ccgttgaacg agtatttggt
     2341 cattcaaatc cgcaacagta tggcgcagat tatgcgccgg tttgattacg ggatgaccgc
     2401 gcttgcctcc atcggcgcga ccgcgccgtt tatcgggctg ttcggcacgg tttgggggat
     2461 ttaccacgcc ctgatcaata tcgggcaaag cgggcagatg agtattgcgg cggttgccgg
     2521 cccgattggc gaggcactgg tggcgacggc ggcgggtttg ttcgtggcga ttccggcggt
     2581 gttggcatac aacttcctca atcgcggcac aaaaatactg acccaggatt tggatgcgat
     2641 ggcgcacgat ttgcacgtcc gcctgcttaa tcaaaaggat agctgattat ggcatttggt
     2701 tcgatgaatt ccggcgacga ttctccgatg tccgacatca acgttacgcc gttggtggac
     2761 gtgatgctgg tgttgctgat tgtgtttatg attactatgc cggtgctgac gcattccatc
     2821 cctttggaac tgccgaccgc gtccgagcag acaaacaagc aggacaaaca gcctaaagac
     2881 cccctgcgcc tgacgattga tgcgaacggc ggctattatg tcggcgggga ttctgcaagc
     2941 aaagtggaaa tcggggaagt ggaaagccgt ctgaaagccg ccaaggagca gaatgaaaac
     3001 gtgattgtgg cgattgcggc agacaaggcg gtggaatacg attatgtaaa caaagcttta
     3061 gaagccgccc gtcaggcagg aatcaccaaa atcggttttg taaccgaaac caaggcgcaa
     3121 taaagttcga cgcttgaacg aatgccgtct gaaagtcttt cagacggcat ttcttatgtt
     3181 taatcagcta tatatagttg attaaaacaa aaatgagacg acagagcacc gtctccattt
     3241 ctgcgcaggc gggaatacaa acatctgatt tttcttttat tttcaaacat tactgaaatg
     3301 ggtaagtctg gagtcctgcc tgcgcgagaa taatgaaaat gtgcatttct aatttttata
     3361 gtggattaac aaaaatcagg acaaggcaac gaagccg
//
LOCUS       DAXHNE010000178         3299 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.178, whole genome shotgun sequence.
ACCESSION   DAXHNE010000178 DAXHNE010000000
VERSION     DAXHNE010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.178"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(184..405)
                     /locus_tag="ACFM4Z_001909"
     CDS             complement(184..405)
                     /locus_tag="ACFM4Z_001909"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021887.1"
                     /translation="MLSTRKTARKIQARHIALPFSGKPQFYRPQERLSKQAQATPPGH
                     FRFHRLSVVRIMQQHHQRITLFGRQQAEI"
     gene            464..1087
                     /locus_tag="ACFM4Z_001910"
     CDS             464..1087
                     /locus_tag="ACFM4Z_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC6021888.1"
                     /translation="MRKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1198..2580
                     /locus_tag="ACFM4Z_001911"
     CDS             1198..2580
                     /locus_tag="ACFM4Z_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6021889.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYAGLVVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFMLTGILTVISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNQEGLYRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2584..3108
                     /locus_tag="ACFM4Z_001912"
     CDS             2584..3108
                     /locus_tag="ACFM4Z_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC6021890.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          681 a          865 c          994 g          759 t
ORIGIN      
        1 aggattaaag gggtatttgg gtaaaattag gaggtatctg gggcgaaaac agccgaaaac
       61 ctgtgtttgg gtttcggctg tcgggaggga aaggaatttt gcaaaggcct caagatatag
      121 tggattaaat ttaaatcagt acggcaaggc gaggcaacgc tgtactggtt taaatttaat
      181 ctactatatt tccgcctgct gccgcccgaa aagcgtgatg cgctgatggt gctgctgcat
      241 aatccggacg acggataacc ggtgaaaacg gaaatgcccg ggcggtgttg cctgtgcctg
      301 tttggacaag cgttcttgag ggcggtagaa ttgaggtttg cccgaaaagg gcagggcgat
      361 atgccgcgct tggattttgc gcgcggtttt tcgcgttgaa agcaattatg tctgtttgaa
      421 tatttaatgc cgtctgaaac gtccggacgc gcccccgttc cctatgagaa aaccgaccga
      481 taccctaccc gttaatctgc aacgccgccg cctgttgtgt gccgccggtg cgttgttgct
      541 cagtcctctg gcgcacgccg gcgcgcaacg tgaggaaacg cttgccgacg atgtggcttc
      601 cgtgatgagg agttctgtcg gcagcgtcaa tccgccgagg ctggtgtttg acaatccgaa
      661 agagggcgag cgttggttgt ctgccatgtc ggcacgtttg gcaaggttcg tccccgagga
      721 ggaggagcgg cgcaggctgc tggtcaatat ccagtacgaa agcagccggg ccggtttgga
      781 tacgcagatt gtgttggggc tgattgaggt ggaaagcgcg ttccgccagt atgcaatcag
      841 cggtgtcggc gcgcgcggcc tgatgcaggt tatgccgttt tggaaaaact acatcggcaa
      901 accggcgcac aacctgttcg acatccgcac caacctgcgt tacggctgta ccatcctgcg
      961 ccattaccgg aatcttgaaa aaggcaacat cgtccgcgcg cttgcccgct ttaacggcag
     1021 cttgggcagc aataaatatc cgaacgccgt tttgggcgcg tggcgcaacc gctggcagtg
     1081 gcgttgattt tgaacccgcg ccgcaaccga aatacggcga atcctgtata atccgaaaat
     1141 ctgttcactg gaagttcaga cggcattgca actgttgatg ccgtctgaaa aaatatgatg
     1201 gcaagagaca accgcatcca aatgtttccg cacgaatggc gcgccagtac gacgctttcc
     1261 ggcgtgtacg cgctgcgtat gctgggtatg ttcctcgtgc tgcccgtatt ggcggtgtat
     1321 gccgcctcgc tgcccggcgc ggaaggcaac aaaacgctgg tcgggctggc aatgggcatt
     1381 tacgggctga cacaggctct gctgcaactg cctttgggca tcgcttccga caagttcggg
     1441 cgcaagaaaa ccatttatgc gggactggtc gtgtttgcgg cgggcagctt tcttgccgcc
     1501 gccgccgata cgctgcccat gctggtcgcc gcacgcgcca tacagggtgc aggggcggtc
     1561 agtgcggcgg ttaccgcgct gctggcggat ttgacgcgcg acggcgtacg tacccgcgca
     1621 atggcgatga tcggtttgag tatcggtttg acgttttcgg tcagcctcgt cgttgccccc
     1681 gtgattgccg acgcggtcgg cgttcgcgga ctgtttatgc tgaccggcat tctgaccgtc
     1741 atcagcatcg gcgtggtggc gtggatgact cccgatcccg aagtttccaa gctgcacgaa
     1801 gatacgcagg cgcagccttc gcgcataggc gaagttttga aaaaccgtag gctgctgacg
     1861 cttgatttcg gcattttcgc cctgcacgcc gcacaaatgg cattgtttac cgcgctgcct
     1921 ttcgcgatga cccagctcgg tttggaaaaa atacagcatt ggaaagtcta tctgccttcg
     1981 accattacgg gcttggtggt gatggttccg ctgattatcg tcggcgagac gcgcaacaag
     2041 cttaagcagg tttttgtttt gggtatcgtc tgtattgcgg cggcgcagct cggtttgctg
     2101 tccggtatgc gctcggtagg cttgattacc gcttatttgg ttgtttactt tatcggtttt
     2161 aatgtgttgg aagcgagcct gccgtctatg gtttccaaaa tcgcgccgtc cgacctgaag
     2221 ggtacggcga tgggcgtgta caacacgatg cagtcgctcg gactgtttgc cggcggcgcg
     2281 gcaggcggtt tgctgtttca aaaatacggc ttttccggcg tgtttgcctt ttgcagtata
     2341 ttgatgctgc tgtggctggt aattgccgtt ttatcgcctg cgcccaagcc cgtcaaaaac
     2401 ctcagttacc ctgtcggcgg cgtgtggcag ggcaatcagg aagggttata ccgcgccttg
     2461 tcggagcttg agggtgtgga agacatcggt ttcagtttcg acgggcagac cgtctatctc
     2521 aaagtgttgc agaagggttt cgatcaggct gccgctgaaa aaatcatcac aggagtttaa
     2581 aaaatgtcat tgaacaaagt catcctcatc ggccgcctcg gacgcgatcc cgaagtgcgc
     2641 tatatgccca acggcgaggc ggtttgtaat ttcagcgtcg ccaccagcga aacttggaac
     2701 gaccgcaacg gccaacgtgt agagcgtact gagtggcaca acatcaccat gtaccgcaaa
     2761 ctggcggaaa ttgccgggca atacctcaaa aaaggcgggc tggtttattt ggaaggcaga
     2821 atccaaagcc gcaaatacca aggcaaagac ggcatcgaac gcaccgctta cgatattgtc
     2881 gccaacgaaa tgaaaatgtt gggcgggcgc aatgaaaaca gcggcggtgc gccttacgag
     2941 gaaggttacg gtcagagtca ggaggcttac caacgccccg cgcagcaaag ccggcagccc
     3001 gcctccgacg cgccgtccca tccccaagaa gcaccagccg cgccgcgccg ccaacccgtg
     3061 cctgccgccg ccccggtcga ggacattgac gacgatatcc cgttctgaat tttacggccg
     3121 gacatcctcg cggagggaaa tcataaagga cggagaaacc ttaaacctta cggggcaggg
     3181 tttctctttt ttgcatttgg gccggcttgt tgccgccctt tgcgttgggc ggaacgttgc
     3241 gattgcgccg ttcgggcgtt ttgaggcagt gatgcctgcc gcacgtcccc gctgttttc
//
LOCUS       DAXHNE010000179         3299 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.179, whole genome shotgun sequence.
ACCESSION   DAXHNE010000179 DAXHNE010000000
VERSION     DAXHNE010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.179"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            83..444
                     /locus_tag="ACFM4Z_001913"
                     /pseudo
     CDS             83..444
                     /locus_tag="ACFM4Z_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239093.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            441..1097
                     /locus_tag="ACFM4Z_001914"
     CDS             441..1097
                     /locus_tag="ACFM4Z_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HFC6021891.1"
                     /translation="MILHPSSFNYRDIPKGADFSDNDFVRHFETLVEGRYYIANAWTK
                     IVRREIIIKNNLFFPKGYIHEDFPYSLQLARFIKTFAFYDNPFYQYRVLGGSISHNIK
                     YKNFSDVLTHLDRGVDFLVENKNSPIYGGLQKFVFDNIGYLRSISVRLYFSKNIIVVY
                     REYFSFKEKCRKIFGAKAIRPIFIGKTAFIIGLPILRLLVPPMLYPAIKAVYQKFFSE
                     "
     gene            complement(1076..1204)
                     /locus_tag="ACFM4Z_001915"
     CDS             complement(1076..1204)
                     /locus_tag="ACFM4Z_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021892.1"
                     /translation="MPSEAGFVASDGIAAIPAGGAAVSSRPLPIRGLPEAYSEKNF"
     gene            1313..2650
                     /gene="ribBA"
                     /locus_tag="ACFM4Z_001916"
     CDS             1313..2650
                     /gene="ribBA"
                     /locus_tag="ACFM4Z_001916"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFC6021893.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHKLKIGTIT
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADEETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERIQQAESGVVILLHRTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILVGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(2810..2926)
                     /locus_tag="ACFM4Z_001917"
     CDS             complement(2810..2926)
                     /locus_tag="ACFM4Z_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC6021894.1"
                     /translation="MPSESLSDGILFGLENTYAVLSLKEKIQKTKATGIYRK"
     gene            complement(2958..>3299)
                     /locus_tag="ACFM4Z_001918"
     CDS             complement(2958..>3299)
                     /locus_tag="ACFM4Z_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFC6021895.1"
                     /translation="IDASGLGEKIKDGKNQKTVLSREEEQKICNTFTHKQAVEDFSVV
                     IGYDEIKAKNHSLSAWQYFEVKIDYVDISADEFAQKMAGFSADLDKLFAESAELEKEI
                     KDRLAMLKFNS"
BASE COUNT          863 a          906 c          738 g          792 t
ORIGIN      
        1 ttacagggtg cggatgcaaa cgcattgcga gcgcgggttt gaggcatacg cgcaaacatc
       61 ttaatataat ggattgatat ttatgatttt ctccatcatc gtccctattt acaatgtgga
      121 aaatacctcc gctgctgcgt ggattccgtg cttgccgaaa attttgccga ttatgaaatg
      181 attttggtcg atgacggttc gccggacggc tgcgggaaga tttgcgacga atatgcaggc
      241 aaatatccgc atataaaagt gattcatcaa gaaaacggcg ggctgtcgga tgcccgcaac
      301 gccggtatcc gggcggcaaa aggcgattac ctaatctttt tggacagcga cgactattgg
      361 gccgacacca atcgttcaaa aaacgcgcgg ggggggggat tctctttgat ttacaacaac
      421 ttgcagacaa aaaggttgat ttgatcctgc atccctcgtc cttcaattac cgcgacatcc
      481 ccaaaggggc ggacttttcg gataatgatt ttgtccgcca ttttgaaacg ctggtggagg
      541 ggcggtacta tatcgccaac gcgtggacaa aaatcgtcag gcgggaaata atcatcaaaa
      601 acaatctgtt tttcccaaaa ggatacattc acgaggattt cccgtacagt ttgcaattgg
      661 cgcgttttat caagactttt gccttttacg ataacccttt ttaccagtac cgcgttctcg
      721 gcggctctat cagccacaac atcaaataca aaaatttcag cgatgtgctg acgcatctcg
      781 accggggtgt ggatttttta gtcgaaaaca aaaattcccc catctacggc ggtttgcaaa
      841 aatttgtctt cgacaatatc ggctatctga gatctatatc ggtaaggctt tatttttcca
      901 aaaacattat cgttgtctac cgagaatatt tttcatttaa agagaaatgc agaaagatat
      961 tcggcgcgaa ggcaatccgt ccgattttta tcgggaagac cgcattcatc ataggattgc
     1021 cgatattgcg cctgctcgta ccgcctatgc tgtacccggc aatcaaggcc gtttatcaga
     1081 aatttttttc ggaataagct tccggcaaac cccgaatcgg aagcgggcgg gaagaaacag
     1141 ccgccccgcc ggcggggatt gcggcaatgc cgtctgaagc cacgaatccg gcttcagacg
     1201 gcatctgttt accaaaaagc aaataattcg gtttggcgaa aaaaacagat ttgctttttg
     1261 gtaaatacgc gattacaatc cgctacatcc gatttctaca aaggatgaaa cgatgaccga
     1321 cacagctggt ctgcgccgcc gcaacctgcg gcaatggata gcagaacact acggcggttt
     1381 gcaaacccgt tttgccgaag ccgttgccct caacacaggc gaactctccg cccttttgaa
     1441 aaacaaatcc ttcggcgaga aaaaagcccg taaaatcgaa caggcggcaa aaatgcccgc
     1501 cttctggctc gataccgaac acaccgcccg cccgcccgaa cacacaggaa aacacaccat
     1561 gtcccatatt tcccccatcc ccgaaatcct agccgacatc aaagccggca aaatggtcat
     1621 catcaccgat gccgaagacc gagaaaacga aggcgacctg ctgatggcgg cgcaattcgt
     1681 cacgcccgaa gccatcaact tcatgatcaa acacgcgcgc ggcttggtct gcctgccgat
     1741 ggacggcgaa atggtcgaaa aactcgggct gccgatgatg acccaaaaaa acggcgcgca
     1801 atacggcacc aactttaccg tctccatcga agccgcacac ggcattacca ccggcatttc
     1861 cgccgccgac cgcgccctga ctattcaaac cgccgtttcc ccgaccgcca aacccgaaga
     1921 catcgtccaa cccggtcata tctttccgct tcgcgcccaa aaaggcggcg tactcgtccg
     1981 cgccggacac accgaagccg gcgtcgacct ggcgcaaatg aacgggctga ttcctgccgc
     2041 cgttatttgc gaaatcatca acgacgacgg cacgatggcg cgtatgcccg aactgatgaa
     2101 attcgccgaa gaacacaagc tcaaaatcgg caccattacc gacctcatcg aataccgcag
     2161 ccgtaccgaa agcctgcttg aagacatggg caacgcaccc gtacaaaccc cgtggggcga
     2221 gttccaacaa cacgtttacg tcgacaagct ctccggcgaa acccacctcg ccctcgtcaa
     2281 aggtacacct tccgccgacg aagaaaccct cgtccgcgtc cacgaaccct tcagcgtcat
     2341 ggacttcatc caagccaacc cgcgtcattc atggtcgctg cccaaagcac tcgagcgcat
     2401 ccaacaggcc gaaagcggcg tagtcatcct tctgcaccgc accgaagacg gcgcatccct
     2461 gctcgaccgc accctaccca aaggcgcaaa ccaagcctac aaatgggaca gcaaaagcta
     2521 cggcatcggc gcacaaatcc tcgtcggcct caacgtcaaa aaactgcgcg tcctcgggca
     2581 gccctcatct tttaccggcc tgaccggctt cggtttggaa gtcgtcggct ttgaagaagc
     2641 ggaaaaataa tatagtaaat tcaaatactt tatattttct ttatttattg cattatttcc
     2701 gtgcaaacga aaacccggtc tgttgggttg gattttgttt ttgcaaattt cgggtaactt
     2761 ctaattcgtc attcccgcgc aggcgggaat ccggttcgtc gggtttttgt catttccgat
     2821 aaattcctgt ggctttggtt ttttggattt tctctttcag ggaaagaacg gcataagtat
     2881 tttccaaacc aaacaaaatg ccgtctgaaa ggctttcaga cggcatttta agtttgaccg
     2941 gtttcattca tcggtattta tgaattgaat ttcaacatcg ccaatctatc cttaatctct
     3001 ttttccaatt cggcagattc ggcgaaaagt ttatccaaat ccgctgaaaa tcccgccatt
     3061 ttttgcgcaa attcgtcggc ggaaatatcc acataatcaa tctttacctc gaaatactgc
     3121 cacgccgaca agctgtgatt cttcgctttg atttcatcgt agccgatcac cacgctgaaa
     3181 tcttccactg cttgtttgtg cgtgaaagta ttgcagattt tttgttcttc ttcgcgagaa
     3241 agtacggttt tttggttttt gccgtctttg attttttcgc ccaagcccga tgcgtcgat
//
LOCUS       DAXHNE010000180         3234 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.180, whole genome shotgun sequence.
ACCESSION   DAXHNE010000180 DAXHNE010000000
VERSION     DAXHNE010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.180"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(66..2225)
                     /gene="fetA"
                     /locus_tag="ACFM4Z_001919"
                     /gene_synonym="frpB"
     CDS             complement(66..2225)
                     /gene="fetA"
                     /locus_tag="ACFM4Z_001919"
                     /gene_synonym="frpB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021438799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor FetA/FrpB"
                     /protein_id="HFC6021896.1"
                     /translation="MNTPLFRLSLLSLTLAAGFAHAAENNAKVVLDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTSFGGDDHRIVLSHMKDQHRGIRTVREEFTVTNNSRLTLARQ
                     APAYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKKRYSADDKDNGYAGNVKGPNHT
                     RITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSKFSIPTTEKKNGQDVAKP
                     ADQQAKDRKDEALVHSYRLTNPTKTDTGAYIEAIHEINGFTLTGGLRYDRFKVKTHDG
                     KTVSSSNLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIADGTKA
                     ERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHVSAAVREAVNAGYIKNHGY
                     ELGASYRTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYRFKNP
                     NLEIGWRGRYVQKAVGSILVAGQKDRNGKLENVVRKGFGVNDVFANWKPLGKDTLNVN
                     LSVNNVFNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
     gene            complement(3038..>3234)
                     /locus_tag="ACFM4Z_001920"
     CDS             complement(3038..>3234)
                     /locus_tag="ACFM4Z_001920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021897.1"
                     /translation="PKLNGLDSRFRGNDGDRVAVIVDEQKPVRRCLALAQRERFSKVL
                     KHQVNRFRPICTVCGFVALS"
BASE COUNT          724 a          744 c          930 g          836 t
ORIGIN      
        1 atcaaattat caggacagat gccgtctgaa aggctttcag acggcatttt ttcgggatgt
       61 gcgttttaga acttgtagtt cacgcccaag cgtacatcac gtcccacgcc cggcagggta
      121 ttggtccagc gttggctgtg cggatagtag aacgtgttga acacgttgtt aaccgaaaga
      181 ttaacattga gcgtgtcttt gcccagcggt ttccagttgg cgaagacatc gttcacaccg
      241 aaacctttgc gtacaacgtt ttccaatttg ccgttgcggt ctttttgacc tgccaccaat
      301 atcgaaccca cggctttttg aacataacgg ccgcgccagc cgatttccag attcgggttt
      361 ttgaagcggt aggcaaggga ggctgtccaa gtgcggccga cttgtgcgcc aaattcagga
      421 ttcgcgctca acagcttgtc tttgtgcgta tcgtaaaagc gcggtttgct gtggcttacg
      481 ccgactttgg cagtcaggcc gccggtgcgg taggacgcgc ccaattcgta accgtggttt
      541 ttgatgtaac cggcattgac ggcttcacgg acagcggcag agacgtggcg gttttgcgga
      601 ttggcaagcg cgtctttgat ggtttgccag aagtagctgc cgttggcggc aaacgtgccg
      661 tcgttgtagt tgaagccgat ttcggtattg cgcgcgcgtt cggctttcgt gccgtcggca
      721 atcgagatga tgccgcgttt accgtgggtt tgcagcgcgt catacaggcg cgggctgcgg
      781 ctggcgtagt tgtggctcgc gctgaagctc cagtgttcgt gcggctgcca aatcacgccg
      841 aaactcgggt taaggttgct gcttgaaacg gttttgccgt cgtgggtttt caccttgaag
      901 cggtcgtaac gcagcccgcc ggtcagggta aagccgttaa tttcgtgaat ggcttcgata
      961 tacgcgccgg tatcggtttt ggtcgggttg gtcagacggt aggaatggac aagggcttcg
     1021 tctttgcggt ctttggcttg ttggtctgct ggtttagcaa catcttgacc gtttttcttt
     1081 tctgtcgtcg ggatggagaa tttcgagttc aaaaacgctt gcggtttgat ttcctgatgg
     1141 cggtagttga taccgtattt caacagggtt tgttcggcaa ggcggctgtc gaagttgaag
     1201 ttcatacccc gagtggtgat tcgggtatgg ttggggcctt ttacattgcc tgcgtagccg
     1261 ttatctttgt catcggcgga atagcgtttc ttttccaaca cataggcgtt ggcatccagt
     1321 ttttcgacaa agcccaaatc tttgccggtg tacgccaagt tggtgttgga ttgagtggtt
     1381 tcgcggtaag caggtgcttg gcgtgctaag gtcagacgtg agttattagt gacggtaaat
     1441 tcttcacgca cagtgcggat gccccggtgt tggtctttca tatggctcaa cacgatgcgg
     1501 tggtcgtcgc cgccgaagct tgttccgatt ttggcgaggt agctgcgttt gtccagcgcg
     1561 ctgtacggta cggttttgcc gccgttgaca ttgcggaaac ctttgccggc ttcgtaatct
     1621 ttttcatcgt tgcggttgta agagaacaag ccgtcgaagt tgccctcttt tccgaatacg
     1681 cttgcgccgt agcttacgcc ttcgttgctg gcaaagccgc tgttgaggcg cacgccccag
     1741 tttttatcca agcctttgag caggtcttgg gcatcgacgg ttttggcgat aatcgcgccg
     1801 ttggtcgcgc cgataccggc agaggcggaa cccgcgcctt tttgtacgga aacgacttta
     1861 accaaagcgg gatcgacaat aaatctgcct tggtggtaaa ggatttggct gtcggaatag
     1921 gcgttgtcca ccttgatgtc gacagagttc tgacccatgc cgcgcagcgt caggaattgg
     1981 gacgtgccgt tgccgccgcc gaaatcgatg gagggctctt cttttaagag ttcgcgcata
     2041 tcggttgcgg tgctttcgtc tttttgttga agcgtaacga tgttggtacg gattttgctg
     2101 ccttggcggt cgccttttac agtaacggta tccagtacga ccttggcatt attttctgcc
     2161 gcgtgggcaa aacctgccgc cagggtaagc gagagcaggc tgagacggaa caatggggta
     2221 ttcattcaat cgtcctcttg agtatatgaa gggaagttaa gccaaaccgt taaggtttgg
     2281 caggataaga aaaataataa ttatttttgt ttatattaac gagagggggg atttgtgtaa
     2341 agctgattat cgtttttatt tgcgaagtgt tgttttttgt tgacaggttt tgtcggaaat
     2401 gtatagtgga ctaacaaaaa tcaggataag gcgacgaagc cgcagacagt acagatagta
     2461 cggcaaggcg aggcaacgct gtactggttt ttgttagtcc actataaaaa acggcggaaa
     2521 tatagtggat taacaaatgc gggaatgacg aaaagcaacc tttccgcgtc attcccgcga
     2581 aagcgggaat ctagaacccc taacgcggca ggaatctatc ggaaatgact gaaaccgaac
     2641 agactggatt cccgcctgcg cgggaatgac gaagcctgtg cgggaatgac gaggcctgcg
     2701 cgggaatgac gaaaagcaac ctttccgcgt ccttcccgcg aaagcgggaa tctagaaccc
     2761 ctaacgcggc aggaatctat cggaaaaaac cgaaaccgaa cagactggat tcccgcctgc
     2821 gcgggaatga cgaagcctgc gcgggaatga cgaaaagcaa cctttccgcg tccttcccgc
     2881 aaaagtggga atctaggaac tcaaagctgc aagaatttat cagaaacaac tggaactcaa
     2941 aaaaactaga ttcccgcctg cgcgggaatg acgaagggtt aaagaaatga cgagaaacgg
     3001 cggtaattta tcgggaataa ccgaaaacga acggaaatca ggacaaggcg acgaagccgc
     3061 agacagtaca aataggacgg aaccgattca cttggtgctt gagcacctta gagaatcgtt
     3121 ctctttgagc taaggcgagg caacgacgta ctggtttttg ttcatccact ataacagcaa
     3181 ccctgtcgcc gtcattcccg cgaaagcggg aatccagtcc gttcagtttc ggtc
//
LOCUS       DAXHNE010000181         3216 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.181, whole genome shotgun sequence.
ACCESSION   DAXHNE010000181 DAXHNE010000000
VERSION     DAXHNE010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.181"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..591)
                     /locus_tag="ACFM4Z_001921"
     CDS             complement(<1..591)
                     /locus_tag="ACFM4Z_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC6021898.1"
                     /translation="MDRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADDEKRNGDEKRN
                     ENVFFWKGVALGSGAELRLFGVDDRRAADLVNKVLAEVARLEKMFSLYREDSLISRLN
                     RDGYLTSPPADFLELLSLCRDIHALTEGAFDPTVQVLWKLYADYFAAHPDAETPPPQN
                     SVKEALKRVGFGKVSFDDREIRFAEKGMGLSLNGIAQ"
     gene            complement(602..1396)
                     /locus_tag="ACFM4Z_001922"
     CDS             complement(602..1396)
                     /locus_tag="ACFM4Z_001922"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFC6021899.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQTAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            1780..3114
                     /gene="gdhA"
                     /locus_tag="ACFM4Z_001923"
     CDS             1780..3114
                     /gene="gdhA"
                     /locus_tag="ACFM4Z_001923"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFC6021900.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
BASE COUNT          839 a          910 c          773 g          694 t
ORIGIN      
        1 ttgcgcgatg ccgttcaagg acaaacccat acccttttcg gcaaaacgga tttcacggtc
       61 gtcaaacgag actttgccga agccgacccg tttcagggct tccttcacgc tgttttgagg
      121 cggcggcgtt tccgcatcgg gatgagcggc aaaataatcg gcatacagtt tccacaaaac
      181 ctgaaccgtc ggatcgaatg ccccttccgt cagcgcgtga atatcgcggc acaggctcaa
      241 cagttccaaa aaatccgccg gcggcgaagt cagataaccg tcacggttca gacggctgat
      301 caggctgtct tcacggtaaa ggctgaacat tttttccaaa cgcgccactt ccgccaaaac
      361 cttgttgacc aaatccgccg cacgcctgtc gtccacaccg aacagacgga gctccgcacc
      421 ggaacccagt gcgacacctt tccagaaaaa cacattttca ttgcgttttt catccccgtt
      481 gcgtttttca tcatcggcgg caaaaggatt cggcaggaaa gaaaccgccg cgcccgccgc
      541 cgcaacggcg gcaaccgtca gaaaacgcct gcgcccgaaa tgcctgtcca tatacccctc
      601 ctcagaccga cactgccgcc ttgatgtgcg gatgggggtc gtagccttcc aactcgaaat
      661 cttcaaactt gaaggaaaac aaatctttga cttcaggatt gattttcatc actggcaagg
      721 cgcgcggttc gcgttccaac tgcaatgcgg tctgctcgaa atggttgcgg tacaaatgcg
      781 cgtcgccaaa cgtatggaca aactcgcccg cctccagccc gcacacttgc gccatcatca
      841 tggtcaacaa tgcgtagctg gcaatattaa acggcacacc gaggaaaata tccgcgctgc
      901 gctggtaaag ctggcaggac agtttgccgt cggcaacgta aaactgaaac agcgcgtggc
      961 agggcggcaa ggccatttca tcgaccaaag ccggattcca cgccgataca atcaggcggc
     1021 gcgagtcggg gtttttctta atttgttcca ccacattggc gatttggtcg atatgcctgc
     1081 cgtcgggcgc gggccagtta cgccactggt agccgtaaac cgggcctaag tcgccgtttt
     1141 cgtccgccca ctcgtcccaa atggaaacat tgttgtcctt taggtatttg atattggtat
     1201 cgcctttgag aaaccaaagc agctcgtgga taatcgagcg caaatgcagc tttttggtcg
     1261 tcagcagcgg aaaacctttg cccaagtcaa aacgcatctg ataaccgaat acggagcgcg
     1321 tgcccgtacc ggtgcggtct gatttgtccg taccgttgtc gaggacgtgg cgcatcaagt
     1381 ccaaataggc tttcatagca gtctttcatc aaattaaacg gcgcatattg taacatttcc
     1441 ggataatgcc caaaacacgg atacaggcag gcaggattgt tggcaatttc agtccttttc
     1501 cacagtaaaa cccagtggga aaacaaaatt accttgattg gaatcaaaaa atctagttta
     1561 attacttaga ataaaatttc aataatattt ttatttacga aattaattta tgattattga
     1621 ttaattatcg gcaacaaaca tcaaacatcg aaaacatgga aaaaataatg tcaacaattt
     1681 ttgccaaatc gggcttggca tcagaaaaaa ataggtttat attcccacct acaaatttgt
     1741 tttcccatta gtacactatc aaccaaaagg agtatccgaa tgactgacct gaacaccctt
     1801 tttgccaacc tcaaacaacg caatcccaat caggagccgt tccatcaggc ggttgaagaa
     1861 gtcttcatga gtctcgatcc gtttttggca aaaaatccga aatacaccca gcaaagcctg
     1921 ctggaacgca tcgtcgaacc cgaacgcgtc gtgatgttcc gcgtaacctg gcaggacgat
     1981 aaagggcaag tccaagtcaa ccggggctac cgcgtgcaaa tgagttccgc catcggtcct
     2041 tacaaaggcg gcctgcgctt ccatccgacc gtcgatttgg gcgtattgaa attcctcgct
     2101 tttgaacaag tgttcaaaaa cgccttgacc accctgccta tgggcggcgg caaaggcggt
     2161 tccgacttcg accccaaagg aaaatccgat gccgaagtaa tgcgcttctg ccaagccttt
     2221 atgaccgaac tctaccgcca catcggcgcg gacaccgatg ttccggccgg cgacatcggc
     2281 gtaggcgggc gcgaaatcgg ctacctgttc ggacaataca aaaaaatccg caacgagttt
     2341 tcttccgtcc tgaccggcaa aggtttggaa tggggcggca gcctcatccg tcccgaagcg
     2401 accggctacg gctgcgtcta tttcgcccaa gcgatgctgc aaacccgcaa cgatagtttt
     2461 gaaggcaaac gcgtcctgat ttccggctcc ggcaacgtgg cgcaatacgc cgccgaaaaa
     2521 gccatccaac tgggtgcgaa agtactgacc gtttccgact ccaacggctt cgtcctcttc
     2581 cccgacagcg gtatgaccga agcgcaactc gccgccttga tcgaattgaa agaagtccgc
     2641 cgcgaacgcg ttgccaccta cgccaaagag caaggtctgc aatactttga aaaccaaaaa
     2701 ccgtggggcg tcgccgccga aatcgccctg ccctgcgcga cccagaacga attggacgaa
     2761 gaagccgcca aaaccctgtt ggcaaacggc tgctacgtcg ttgccgaagg tgcgaatatg
     2821 ccgtcgactt tgggcgcggt cgagcaattt atcaaagccg gcatcctcta cgccccggga
     2881 aaagcctcca atgccggcgg cgtggcaact tcaggtttgg aaatgagcca aaacgccatc
     2941 cgcctgtctt ggactcgtga agaagtcgac caacgcctgt tcggcatcat gcaaagcatc
     3001 cacgaatcct gtctgaaata cggcaaagtc ggcgacacag taaactacgt caatggtgcg
     3061 aacattgccg gtttcgtcaa agttgccgat gcgatgctgg cgcaaggctt ctaagcaaac
     3121 gccgccgctc cgcaaacaaa atgccgtccg aaccgcaaat gctgttcaga cggcatttca
     3181 ttatccgccc gttcgaatcg ggtgatactt atagtg
//
LOCUS       DAXHNE010000182         3185 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.182, whole genome shotgun sequence.
ACCESSION   DAXHNE010000182 DAXHNE010000000
VERSION     DAXHNE010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.182"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(75..668)
                     /gene="ribA"
                     /locus_tag="ACFM4Z_001924"
     CDS             complement(75..668)
                     /gene="ribA"
                     /locus_tag="ACFM4Z_001924"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFC6021901.1"
                     /translation="MSELLNYVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(661..1335)
                     /locus_tag="ACFM4Z_001925"
     CDS             complement(661..1335)
                     /locus_tag="ACFM4Z_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021902.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGTASIDRSV
                     LWLEHEDWRLADYLEQNSGNEEGLKRLFMALDSVFSRCKGVRAAAVYALMPSENAAFR
                     LVCLSQQGEGLENIWNLDENVADVSLACRCAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            1489..1573
                     /locus_tag="ACFM4Z_001926"
     tRNA            1489..1573
                     /locus_tag="ACFM4Z_001926"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1523..1525,aa:Leu,seq:tag)
     gene            2093..3127
                     /gene="purM"
                     /locus_tag="ACFM4Z_001927"
     CDS             2093..3127
                     /gene="purM"
                     /locus_tag="ACFM4Z_001927"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFC6021903.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYQNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGVGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
BASE COUNT          809 a          833 c          792 g          751 t
ORIGIN      
        1 ttattgcttt atcaaggtat ggaaacctgt ttcccgaaag gcggcgcagg atgcccgttc
       61 cctgcaactt tgccttattc cgacatcaga tgtcccagct tgtctgcttt ggtttggagg
      121 tagcgtttgt tttcaaggtt ttccccgacg tgcaggggga tgcgttcgac cacgttaatc
      181 cccgcatctt tcagggtttg gattttttcg gggttgttgg tcagcagttt gaccgagcgg
      241 atgcccagat attcgtagat agattgcgcc aaacggaaat cgcgggcatc gacgggcagc
      301 ccgagtgcca aattggcttc aaccgtatcc ataccttggt cttgcagatg ataggcgcgg
      361 attttgttaa tcagcccgat gccgcgtcct tcctgacgca gatagacgat gatgccgcgc
      421 ccctctgtct gtaccgccct catggccgct tcaagttgag gtccgcagtc gcattttctc
      481 gagaacagcg catcgcccgt caggcattcg gagtggatgc gcgtcagaac cggattgccg
      541 tctgaaaaat taccgacggt cagcgcgacg tgttcctgcc cgtttgcctc ttcaaagccg
      601 tgcatcgtaa atacgcccca ttcggtcggc aggcggcagg aagcaacata gtttaataat
      661 tcagacattt tcatctcctt ctgcggcagt gatgcccaaa agctgcttca aaggttcgga
      721 caaagccaag gcaagagcca cccattccac ctgtgctgcc gtacccgcac actctgcgca
      781 ttcaaattcc acatgaacca cgcccaacac accgccactt tccgtgcaga ccggaatgga
      841 aatttgcgcc gccgaagcat gattgcgttc tcccgaaagc tctcccaaat ccaaccaacg
      901 gcgtacatcc gaggcaacat tcatccagcc gctttgcgcg caacggcagg caagtgaaac
      961 atcggcaaca ttttcatcta gattccatat gttttccaaa ccttctccct gttgggacag
     1021 gcaaaccagc cgaaaggcgg cattttcaga cggcatcagg gcataaacgg ctgcggcgcg
     1081 cacgccttta cagcgcgaaa ataccgaatc cagagccatg aaaagccgtt tcagaccttc
     1141 ctcattcccg ctgttttgct ccaaataatc ggcaagcctc caatcctcat gctcaagcca
     1201 caaaaccgaa cggtctatcg aagccgtccc catatccatt accgtctgcg ccgtcagata
     1261 cgccgcccga acctcgtcaa gcggcagctt caaaccctga gtcagcagaa aatccttaat
     1321 caaagcagca ggcataccca aattccgtca ataaaaacaa aaaaccgccc gattccgatg
     1381 tcaggcggac gaaaaacaga ttttaccgcc ccaaacccca agccatcaac agccccgccg
     1441 aaaaccttgc acaaaaaaac aaaatctgta taatcgtcca attcccatgc ggacgtggcg
     1501 aaattggtag acgcaccaga tttaggttct ggcgccgaga ggtgtgagag ttcgagtctc
     1561 tccgtccgca ccaataatga taaataaatt atatagttaa tgcttatcgg gacaaattag
     1621 tgccacaaaa cataagtcgt ctgcttttca gcgggcggct tttttatctg cctatatgtt
     1681 tcaacacgca gaacggtaca taaagtgccg ccatttgttc cgcaccgaat cggaaacggg
     1741 tgcttccgca cgcaatcaga ttcaccaccc ctaatgccgg ggtttcaacc ggaaagaaga
     1801 aaatattgat tttgatgatg gcatcaaaaa accccttccg ctcgtctgcc aaaaggaatg
     1861 gttaagaaaa gcaatgcgtc ttttgatgga acgcgatata taaggaattg aggggaaggg
     1921 caggcgggtt tctgcccgtt aatcctgcgg acggtcccgc caaacccgac acgcgcgggc
     1981 atatggcgga ctgtttacaa tattgtctaa aaatctgaac ctgcaaacgt gaaataaggt
     2041 agaatacgcc ccgttatttt accgtccaat ccccatttac caaggaaaaa cgatgagtac
     2101 ttcattgagt taccgcgatg caggtgtcga tatcgacgca ggcgaccaac tggtcgaaaa
     2161 catcaaaccg tttgccaaac gcacgatgcg cccggaagta ttgggggatt tgggcggttt
     2221 tggcgcattg gtcgaaatcg gcaagaaata tcaaaatccc gtattggtca gcggtacgga
     2281 cggcgtgggt accaagctca agcttgcctt tgattgggat aaacatgata cggtcggcat
     2341 cgaccttgtt gcaatgagtg tcaacgatat tttggttcaa ggggctgagc ccttgttttt
     2401 cttggactat tttgcctgcg gtaaattgga tgttccgcgc gcgaccgatg ttattaaagg
     2461 cattgcccaa ggttgcgaag aatccggttg cgccctgatt ggcggggaaa ctgccgaaat
     2521 gccgggtatg tatcccgtcg gagaatacga tttggcgggt tttgccgtcg gcgtggtgga
     2581 aaaagagaat gtcattaccg gccgcagcat cggcgtaggc gatgtggtat tgggtttggc
     2641 ttccaacggc gcacattcaa acggctattc ccttatccgt aaaatcatcg aacgcgacaa
     2701 tcccgatctg gatgccgagt ttgataatgg caaaaccttg cgggaggctg ttattgcgcc
     2761 gacccgtctg tatgtgaaac cgattcttgc cgctttggaa aaatttacca ttaaaggtat
     2821 ggcacacatt accggcggcg gcattaccga aaacgtgccg cgcgtgttgc ctgaaaacac
     2881 ggttgcacaa atcgatgcta aatcgtggga attgcccaag ctcttccaat ggcttcaaaa
     2941 ggcgggcaat gtggaaaccc aagaaatgta ccgaaccttt aactgcggca tcggcatggt
     3001 cgttattgtt gctgccgaag atgccgatgc ggttcagggt ctcttgggtg aacaaggtga
     3061 aacagtttac cgtttaggtt tgatccgtga gcgtcaggga gacgagcatc aaacccaggt
     3121 tgcctgattg cttctatagc gaaatgccgt ctcaaacatg ggtttggacg gcatttttat
     3181 agagg
//
LOCUS       DAXHNE010000183         3124 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.183, whole genome shotgun sequence.
ACCESSION   DAXHNE010000183 DAXHNE010000000
VERSION     DAXHNE010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.183"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(327..803)
                     /gene="greA"
                     /locus_tag="ACFM4Z_001928"
     CDS             complement(327..803)
                     /gene="greA"
                     /locus_tag="ACFM4Z_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFC6021904.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VIYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            1199..2500
                     /gene="aroA"
                     /locus_tag="ACFM4Z_001929"
     CDS             1199..2500
                     /gene="aroA"
                     /locus_tag="ACFM4Z_001929"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFC6021905.1"
                     /translation="MTESVRLPVARLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVQIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLVDALRIAGADVEYLGKEHYPPLHIGERQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKERAVQSFDLDANHIPDAAMTLAIVALATRQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPETLTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFDVFSSLTETAE"
     gene            complement(2543..3076)
                     /locus_tag="ACFM4Z_001930"
     CDS             complement(2543..3076)
                     /locus_tag="ACFM4Z_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC6021906.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          654 a          837 c          852 g          781 t
ORIGIN      
        1 tgcgcgggaa tgacgaattt taggtttctg attttggttt tctgtttttg agggaatgac
       61 gggatgcagg ttttcttaac cctgcgtcct agattcccgc ttttgcggga atgacggcga
      121 cagggttgct gttatagcgg atgaacaaaa accagtacgg ggttgtctcg ccttagctca
      181 aagagaacga ttctctaagg tgctgaagca ccaagtgaat cggtttcgta ctatctgtac
      241 tgtcttcggc ttcgtcgcct tgtcctgatt tttattaatc cactataatt tcctgcgtgt
      301 gtcgggtgta tcgaaatcaa gccgaatcaa atatatcgga cttcgataat gtcgtattcg
      361 cgcacgccgc ccggggcttg gacttccgcc gtatccccct cttccttgcc gattaaggcg
      421 cgggcgatgg gtgagccgac atagattttg ccctgtttga tgtcggcttc gtcttcgccg
      481 acaatttgat agataacgtg ttcttccgtt tccaaatctt ccagcgtaac cgtcgtaccg
      541 aacacgattt tgccttcggc gtggatttcg gtcggattga tgatgtgggc aacggaaagt
      601 ttgtgttcca gctcggaaat gcggccctcg ataaagcctt ggcgttcttt ggcggcttcg
      661 tattcggcgt tttcggacaa atcgccgtgc gaacgggctt cggcaatcgc ttcgatcact
      721 tcgggacgcg ccacgctttt gagctgctgc aattcctgtt tcagcaattc cgcaccgcgt
      781 acggtcaggg ggattttttg catcggtctt ttttctccat attccggcac accggtttgc
      841 ggcagcaagc ataccgcgta ccgtcttgtt ttgtgcgtcc ggatattaaa ataaaaatac
      901 aagccgcccg gaaaatcggc ggcttgtctg tcgttgaaca gcggctattc taccaaattc
      961 tatgaaattg gcaatcgtgc cgtgccgccg gcaaacgcgc catgtccgca acaaaagctg
     1021 aaaatatgcc gtcaaagaaa ttttagaaac aaaaaattta aaaataatca attttcagca
     1081 taaaaaacca catttacgga ctttaaaacc gaaaatgcca agcctgagat ttttcataca
     1141 gcatttgcac cagtataatg caggctgttt ttatctttaa taatattgac gttttgccat
     1201 gaccgaatcc gtccgcctcc ccgtcgcccg tctcaaacct tccaccgtcg ccctgcccgg
     1261 ctccaaaagc atcagcaacc gcaccctgct gcttgccgcc ttgtccgaca atgcttgcga
     1321 aatccattcc ctgctcaaat ccgacgatac cgaccgtatg ctcgaagcac tcgataaact
     1381 cggcgttcaa atcgaatatc ttgccgaaga ccgtctgaaa gtgcacggca caggcggacg
     1441 cttccccaac cgcactgccg atttgttttt gggcaacgcg ggcacggcgt tccgcccgtt
     1501 aaccgccgct ctggccgttt tgggcggcga ttatcatctg cacggcgtgc ctcgtatgca
     1561 cgaacgtcct atcggcgatt tggtcgatgc gttgcggatt gccggggccg atgtcgaata
     1621 tctcggcaag gaacactatc cgccgcttca tatcggcgaa cgccaagaca acggcgagcg
     1681 cgtgattccg attaaaggca atgtgtccag ccagtttctg accgcccttt taatggcgtt
     1741 gccgctgacc gggcaggcgt ttgaaatccg tatggtcggc gaattgattt ccaagcccta
     1801 tatcgacatt actttaaaac tgatggcgca attcggcgta caggttatca atgaaggcta
     1861 ccgcgtcttc aaaattcccg ccgatgcgca ctaccacgcg cccgaacact tgcacgtcga
     1921 aggcgatgcc tccagcgcgt cctacttcct cgcagccggt ttgattgccg ccacgcccgt
     1981 ccgcgttacc ggtatcggcg caaacagcat acagggcgat gtcgcctttg cccgcgagct
     2041 ggaaaaaatc ggggcggacg tggtttgggg cgaaaacttc gtcgaagttt cacgcccgaa
     2101 ggaacgtgcc gtccaatcct ttgatttgga tgcgaaccat atccccgatg ccgccatgac
     2161 cctcgccatc gtcgcgcttg ctacaaggca aacctgcacg ctgcgcaaca tcggttcgtg
     2221 gcgcgtcaaa gaaaccgacc gcatcgccgc aatggcaaac gagttgcgca aactcggggc
     2281 aaaagtcgtc gaagaagccg aagcaattca catcaccccg cccgaaacgc tgacacccga
     2341 cgccgtcatc gacacgtacg acgaccaccg catggcgatg tgtttctcgc tggtttcgct
     2401 gttgggcgta cccgtcgtca tcaacgatcc gaaatgcacc cacaaaacct tcccgactta
     2461 tttcgacgtg ttctcatcgc tgaccgaaac agcggaataa ggcggcattt tgccgcgatt
     2521 ccggcgcggc gcggcgggcg gctcattctg taaaaaaagt atgtgcgccg aggtagtttt
     2581 tggcgtaaaa cggcgtggag agtttttcgg ttttgatggt tttgccgctg ctgggggcat
     2641 ggatgaattc gccgttgccg atgtagagtc cgacgtgtga gtagcggtgt gcgccgccgg
     2701 tgttgaagaa tacgaggtcg ccggccttga ggcggctgtc gggaattttg cggcttgccg
     2761 ccgccatgtc acgggcggtg cgcggcagct tgacgttgag ggcgtttttg taaacgaatt
     2821 gaatcatgcc gctgcaatcg aagccggttg cggtgctgct gccgccccat ttgtagggcg
     2881 tgccgatgag tccgaggctg tggagcatga gttcctgcga gccttgtgtg cggtcgatgt
     2941 ggctgatgcg gacggcttgg atttgccgga ctgtctgttt gggtttcggt tggcggtgtt
     3001 tgccggaggt cgtgccgcat gaggcgagga gcagtgcgct gagacagagg aaaagggttt
     3061 tgtcgggggg aaacatggtt tttcctttgc gggttcggat atccgtctga aggcgtttca
     3121 gacg
//
LOCUS       DAXHNE010000184         3059 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.184, whole genome shotgun sequence.
ACCESSION   DAXHNE010000184 DAXHNE010000000
VERSION     DAXHNE010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.184"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..929
                     /locus_tag="ACFM4Z_001931"
     CDS             <1..929
                     /locus_tag="ACFM4Z_001931"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6021907.1"
                     /translation="VVNQTWCNLDILIVDDGSTDGTLAIAKDFQKRDSRIKILAQAQN
                     SGLIPSLNIGLDELAKSGGGEYIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLE
                     VLSEEKDGNRLARHHRHGKIWKKPTRHEDIADFFPFGNPIHNNTMIMRRSVIDGGLRY
                     NTERDWAEDYQFWYDVSKLGRLAYYPEALVKYRLHANQVSSKYSVRQHEIAQGIQKTA
                     RNDFLQSMGFKTRFDSLEYRQIKAVAYELLEKHLPEEDFERARRFLYQCFKRTDTPPA
                     GVWLDFAADGRMRRLFTLRQYFGILHRLLKNR"
     gene            971..1810
                     /locus_tag="ACFM4Z_001932"
     CDS             971..1810
                     /locus_tag="ACFM4Z_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC6021908.1"
                     /translation="MQNHVISLASAAERRAHIAATFGRHGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLA
                     EDAWLQERFDPDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISR
                     KAMRFFLDRFAALPPEGLHPVDLMMFSDFFDREGVPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQWRDSPANTFKRRLIRALTKISREREKRRQRREQLIGKIIV
                     PFQ"
     gene            1822..1917
                     /locus_tag="ACFM4Z_001933"
     CDS             1822..1917
                     /locus_tag="ACFM4Z_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HFC6021909.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILHRLLKNR"
     gene            1959..2801
                     /locus_tag="ACFM4Z_001934"
     CDS             1959..2801
                     /locus_tag="ACFM4Z_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC6021910.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSSVEKACFMSHVVLWKQALDEGVPYVAVFEDDVLLGEGAEKFLD
                     EDGWLKERFDPDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKN
                     SMLGSDLEKDREQERRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
BASE COUNT          800 a          737 c          819 g          703 t
ORIGIN      
        1 ccgtcgtgaa tcaaacttgg tgcaacttgg atattttgat tgtcgatgac ggctcgacag
       61 acggtacgct tgccattgcc aaggattttc aaaagcggga cagccgtatc aaaatccttg
      121 cacaagctca aaattccggc ctgattccct ctttaaacat cgggctggac gaattggcaa
      181 agtcgggggg gggggaatat attgcacgca ccgatgccga cgatattgcc gcccccgact
      241 ggattgagaa aatcgtgggc gagatggaaa aagaccgcag catcatcgcg atgggcgcgt
      301 ggctggaagt gttgtcggaa gaaaaggacg gcaaccggct ggcgcggcat cacaggcacg
      361 gcaaaatttg gaaaaagccg acccggcacg aagatattgc cgactttttc cctttcggca
      421 accccataca caacaacacg atgattatgc ggcgcagcgt cattgacggc ggtttgcgtt
      481 acaacaccga gcgggattgg gcggaagatt accaattttg gtacgatgtc agtaaattgg
      541 gcaggctggc ttattatccc gaagccttgg tcaaataccg ccttcacgcc aatcaggttt
      601 catccaaata cagcgtccgc caacacgaaa tcgcgcaggg catccaaaaa accgccagaa
      661 acgatttttt gcagtctatg ggttttaaaa cccggttcga cagccttgaa taccgccaaa
      721 taaaagcagt agcgtatgaa ttgctggaga aacatttgcc ggaagaagat tttgaacgcg
      781 cccgccggtt tttgtaccaa tgcttcaaac ggacggacac gccgcccgcc ggcgtgtggc
      841 tggattttgc ggcagacggc aggatgcggc ggctgtttac cttgaggcaa tacttcggca
      901 ttttgcaccg attgctgaaa aaccgttgaa aaacgccgct ttatccaaca gacaaaaaac
      961 aggataaatt atgcaaaacc acgttatcag cttagcttcc gccgcagaac gcagggcgca
     1021 cattgccgca accttcggca ggcacggcat cccgtttcag tttttcgacg cactgatgcc
     1081 gtctgaaagg ctggaacagg caatggcgga actcgtcccc ggcttgtcgg cgcaccccta
     1141 tttgagcgga gtggaaaaag cctgctttat gagccacgcc gtattgtgga aacaggcatt
     1201 ggacgaaggt ctgccgtata tcgccgtatt tgaggacgac gttttactcg gcgaaggcgc
     1261 ggagaaattc cttgccgaag acgcttggct gcaagaacgc tttgaccccg attccgcctt
     1321 tatcgtccgc ttggaaacga tgtttatgca cgtcctgacc tcgccctccg gcgtggcgga
     1381 ctactgcggg cgcgcctttc cgctgttgga aagcgaacac tgggggacgg cgggctatat
     1441 catttcccga aaagcgatgc ggtttttcct ggacaggttt gccgccctgc cgcccgaagg
     1501 gctgcacccc gtcgatctga tgatgttcag cgattttttc gacagggaag gagtgccggt
     1561 ttgccagctc aatcccgcct tgtgcgccca agagctgcat tatgccaagt ttcacgacca
     1621 aaacagcgca ttgggcagcc tgatcgaaca cgaccgcctc ctgaaccgca aacagcaatg
     1681 gcgcgattcc cccgccaaca cattcaaacg ccgcctgatc cgcgccttga ccaaaatcag
     1741 cagggaaagg gaaaaacgtc ggcaaaggcg cgaacagtta atcggcaaga tcattgtgcc
     1801 tttccaataa aaggagaaaa gatggacatc gtatttgcgg cagacggcag gatgcggcgg
     1861 ctgtttacct tgaggcaata cttcggcatt ttgcaccgat tgctgaaaaa ccgttgaaaa
     1921 acgccgcttt atccaacaga caaaaaacag gataaattat gcaaaaccac gttatcagct
     1981 tagcttccgc cgcagaacgc agggcgcaca ttgccgatac cttcggcagg cacggcatcc
     2041 cgtttcagtt tttcgacgca ctgatgccgt ctgaaaggct ggagcaggca atggcggaac
     2101 tcgtacccgg cttgtcggcg catccttatt tgagtagtgt ggaaaaagcc tgctttatga
     2161 gccacgtcgt attgtggaag caggcgttgg acgaaggcgt accgtatgtt gccgtatttg
     2221 aggatgatgt cttactcggc gaaggggcgg agaaattcct tgacgaagac ggctggctaa
     2281 aagagcggtt tgacccggat tccgccttta tcgtccgttt ggaaacgatg tttgcgaaag
     2341 ttattgtcag accggataaa gtcctgaatt atgagaaccg gtcatttcct ttgctggaga
     2401 gcgaacattg ggggacggct ggctatatca tttcgcgtga ggcgatgcgg tttttcttgg
     2461 aaaggtttgc cgttttgccg gcagagtgga ttaaagcggt agattggatg atgtttactt
     2521 atttctttga taaggagggg atgcctgttt atcaggttaa tcccgcctta tgtacccaag
     2581 aattgcatta tgccaagttc ctcagtaaaa acagtatgtt gggtagcgat ttggaaaaag
     2641 atagggaaca agaaagaaga caccgccgtt cgttgaaggt aatgtttgac ttgaagcgtg
     2701 ctttgggtaa attcggtaga gaaaagaaga aaagaatgga gcgtcaaagg caggcggagc
     2761 ttgagaaagc ttacggcagg cgggtcatat cgttcaaata gtttgtgtaa aatatagcgg
     2821 attaaaatta gaaatggaca cactgtcatt cccgcgcagg cgggaatcca gtccgttgag
     2881 tttcagttat ttccgataaa tcaccgcaac gttaaatttc tagattcccg cttttgcggg
     2941 aatgacggcg ggggatttct gtttttccga caaattccta aaacttaaaa ttccgttatt
     3001 cccgcgaaaa cagaaaacca aaaatagaaa cctaaaatct cgtcattccc gcaaaaaca
//
LOCUS       DAXHNE010000185         2964 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.185, whole genome shotgun sequence.
ACCESSION   DAXHNE010000185 DAXHNE010000000
VERSION     DAXHNE010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.185"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..571
                     /locus_tag="ACFM4Z_001935"
     CDS             <1..571
                     /locus_tag="ACFM4Z_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFC6021911.1"
                     /translation="GASKSMVERILTDIQKADPRWSVILLRYFNPIGAHESGLIGEQP
                     NGIPNNLLPYICQVAAGKLPQLAVFGDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKG
                     GVAGVHLFNLGSGRAYSVLEIIRAFEAASGLNIPYQIQPRRAGDLACSFADPSHTKQE
                     TGWETKRSLAQMMEDSWRWVSRNPDGYGD"
     gene            572..1654
                     /gene="rfbB"
                     /locus_tag="ACFM4Z_001936"
     CDS             572..1654
                     /gene="rfbB"
                     /locus_tag="ACFM4Z_001936"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFC6021912.1"
                     /translation="MQTANKKTILVTGGAGFIGSAVVRHIIRNTQDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIGSAGEF
                     IQTNIVGTFNLLEAARAYWQQMPSEQHEAFRFHHISTDEVYGDLHGTDDLFTETTPYA
                     PSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKP
                     LPVYGDGMQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICTLLE
                     ELVPEKPAGVARYEDLITFVQDRPGHDVRYAVDAAKIRRDLGWLPLETFESGLRKTVQ
                     WYLDNKTWWQNVLNGSYRLERLGTGK"
     gene            1717..2583
                     /gene="rfbA"
                     /locus_tag="ACFM4Z_001937"
     CDS             1717..2583
                     /gene="rfbA"
                     /locus_tag="ACFM4Z_001937"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFC6021913.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQIKPSARGELEITDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQDLQIACLEEIAWRNGWLEKKDVETRAKPLEKT
                     AYGQYLLHLIGK"
     gene            2628..>2964
                     /gene="rfbC"
                     /locus_tag="ACFM4Z_001938"
     CDS             2628..>2964
                     /gene="rfbC"
                     /locus_tag="ACFM4Z_001938"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFC6021914.1"
                     /translation="MNIIDTALPDVKILKPQVFKDGRGFFMETFRDGWFKENIADCSF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWTGATLS
                     AQNQCQLWIP"
BASE COUNT          743 a          830 c          743 g          648 t
ORIGIN      
        1 cggcgcatcg aaatcaatgg ttgagcgcat tctcactgat attcaaaaag ccgatccgcg
       61 ctggagcgtg attttgttgc gttatttcaa tccgattggc gcgcacgaaa gtggtttgat
      121 tggcgagcag ccaaacggca tcccgaataa tttgttgcct tatatctgcc aagtggcggc
      181 aggcaaactg ccgcaattgg cggtatttgg cgatgactac cctacccccg acggcacggg
      241 gatgcgtgac tatattcatg tgatggattt ggcagaaggc catgtcgcgg ctatgcaggc
      301 aaaaggcggc gttgccggcg tacatttgtt caacttgggt tcggggcgcg cctattccgt
      361 tttggaaatc atccgtgcct ttgaagccgc atccggctta aatattccct atcaaatcca
      421 accccgacgc gccggcgacc tcgcttgttc ctttgccgac ccgtcccata ccaaacaaga
      481 gacaggctgg gaaaccaaac gcagccttgc acaaatgatg gaagattcgt ggcgttgggt
      541 cagccgcaac cccgacggat acggggatta aatgcaaacc gcaaataaaa aaaccatcct
      601 cgttaccggc ggtgcgggct ttatcggctc tgccgttgtc cgtcatatta tccgaaacac
      661 ccaagattcc gtcgtcaacc tcgacaaact gacctacgcg ggcaatctcg aatcgctgac
      721 cgacattgcc gataatcccc gctatgcttt cgagcaagtg gacatttgcg accgcgccga
      781 actcgaccgc gtattcgcac aacaccggcc cgatgccgtg atgcacttgg cggcggaaag
      841 ccatgtcgac cgctctatcg gttcggcagg cgagtttatc caaaccaata tcgtcggcac
      901 attcaatctg cttgaagcag cccgcgccta ctggcaacaa atgccgtctg aacagcacga
      961 agccttccgt tttcatcata tttcgactga tgaagtgtac ggcgatttgc acggcacgga
     1021 tgatttgttc accgaaacca cgccttacgc cccttccagc ccctattcgg cttccaaagc
     1081 ctccagcgac catttggtgc gcgcatggct gcgtacttac ggcctgccga ccattgtaac
     1141 caattgttcc aacaactacg gcccttatca tttccctgaa aaactgattc cgctgatgat
     1201 tctgaatgcg cttgatggca aaccgctgcc tgtgtacggc gacggtatgc aaatccgcga
     1261 ctggctgttt gtcgaagacc acgcgcgcgc actgtatcag gttgttaccg aaggtgttgt
     1321 cggcgaaacc tacaatatcg gcggccacaa tgaaaaagcc aatattgaag tcgtcaaaac
     1381 catctgcacc ctgctggaag agcttgttcc tgaaaaaccg gccggtgtgg cgcgttatga
     1441 agatttgatt actttcgtac aagaccgccc cggccatgac gtacgctacg ccgtcgacgc
     1501 agccaaaatc aggcgggatt tgggctggct gcctttggaa accttcgagt ccggcctccg
     1561 caaaaccgtg caatggtatc tggacaacaa aacctggtgg caaaatgtat tgaacggcag
     1621 ctatcgtttg gaacgtttag gtactggaaa atagttttca gacggcatcc cgacgcaatg
     1681 ccgtctgaaa acccatcgca aaggaagaaa gaaaagatga aaggcatcat actggcaggc
     1741 ggcagcggca cgcgcctcta ccccatcacg cgcggcgtat ccaaacagct cctgcccgtg
     1801 tacgacaaac cgatgattta ttaccccttg tcggttttga tgctggcggg aatccgcgat
     1861 attttggtga ttaccgcgcc tgaagacaac gcctctttca aacgcctgct tggcgacggc
     1921 agcgatttcg gcatttccat cagttatgcc gtgcaaccca gtccggacgg cttggcacag
     1981 gcatttatca tcggcgaaga atttatcggc aacgacaatg tttgcttggt tttgggcgac
     2041 aatatttttt acggtcagtc gtttacgcaa acattgaaac aggcggcagc gcaaacgcac
     2101 ggcgcaaccg tgtttgctta tcaggtcaaa aaccccgaac gtttcggcgt ggttgaattt
     2161 aacgaaaact tccgcgccgt ttccatcgaa gaaaaaccgc aacggcccaa atccgattgg
     2221 gcggtaaccg gcttgtactt ctacgacaac cgtgccgtcg agttcgccaa acaaatcaaa
     2281 ccgtccgcac gcggcgaatt ggaaattacc gacctcaacc ggatgtattt ggaagacggc
     2341 tcgctctccg ttcaaatatt gggacgcggt ttcgcgtggc tggacaccgg tacgcacgaa
     2401 agcctgcacg aggcggcttc gttcgtccaa accgtgcaaa acatccaaga cctgcaaatc
     2461 gcctgtctgg aagaaatcgc ctggcgcaac ggctggctgg agaaaaaaga tgtggaaaca
     2521 cgcgcaaagc ctttggaaaa aaccgcctac gggcaatacc tgctgcacct gatcggcaaa
     2581 taaatgccgt ctgaaccact ttcttcagac ggcatacaga gaaaatcatg aacatcatcg
     2641 acaccgccct ccccgacgta aaaattttaa aaccgcaagt cttcaaagac gggcgcggct
     2701 tttttatgga aaccttccgc gacgggtggt tcaaagaaaa tattgccgac tgttcctttg
     2761 ttcaggaaaa ccactccaaa tccggcaaag gcgtattgcg cggcctgcac taccaaaccg
     2821 aaaacacaca aggcaaactc gtacgcgtgg ttgtcggcga agtgttcgac gtggccgtcg
     2881 atatgcgcga aggttcgccg actttcggca aatggacagg cgcaaccctg tcggcacaaa
     2941 accaatgcca gctttggata cccg
//
LOCUS       DAXHNE010000186         2941 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.186, whole genome shotgun sequence.
ACCESSION   DAXHNE010000186 DAXHNE010000000
VERSION     DAXHNE010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.186"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            289..612
                     /locus_tag="ACFM4Z_001939"
     CDS             289..612
                     /locus_tag="ACFM4Z_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC6021915.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSLVLIELSD
                     PSEAV"
     gene            726..2903
                     /gene="tdfF"
                     /locus_tag="ACFM4Z_001940"
     CDS             726..2903
                     /gene="tdfF"
                     /locus_tag="ACFM4Z_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent iron piracy receptor TdfF"
                     /protein_id="HFC6021916.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQKSTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLGADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDPSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT          710 a         1038 c          744 g          449 t
ORIGIN      
        1 gcttccttcg aaagctccgt tttcagacga ccttttggaa caccacaggc acacgcatcg
       61 aacggctgaa tcaaagttca gaccgatggc agcccgcacc cgagtttatg cggcaaacag
      121 cgaggctacg gcaacccgtc ccctctccct gtgggagagg gttagggaga gggcggtaag
      181 ccgcaggctt gcatcaaagt cgataacgct ttcgttacaa ctccgcccac tgaacgaagc
      241 caaaacgaca aaccgcatcg taaaccaccc aacccatagg agaaccccat gcaaaacgaa
      301 accatcaacc tgaaacagca ccttgccgcc atcaaagaat actggcagcc cgaaatcatc
      361 aaccgccacg ggttccaatt ccgtttggtc aaacttttgg gcgattacgg ctggcatacg
      421 cacggataca gcgacaaagt gctgtttgcc gtggagggcg acatggcggt ggacttcgcc
      481 gacggcggca gcatgacgat acgcgagggc gagatggcgg tcgtgccgaa gtcggtgtcg
      541 caccgcccgc gttcggaaaa cggctgctcg ttggtgctga ttgagttgtc cgacccgtcc
      601 gaggccgtct gaaaacgaag tttccgaagg aagctgagtt tctgcgaagc taaaagcagc
      661 ctgcaccttc aatcaatatg ccgaaaatac aaccccaccg cacaccaaca cacaaaggaa
      721 atcccatgac acgcttcaaa tattccctgc tgtttgccgc cctgttgccc gtgtacgcgc
      781 aggccgatgt ttctgtttca gacgacccca aaccgcagaa aagcactgaa ttgccgacca
      841 tcaccgttac cgccgaccgc accgcgagtt ccaacgacgg ctacactgtt tccggcacgc
      901 acaccccgct cgggctgccc atgaccctgc gcgaaatccc gcagagcgtc agcgtcatca
      961 catcgcaaca aatgcgcgac caaaacatca aaacgctcga ccgcgccctg ttgcaggcga
     1021 ccggcaccag ccgccagatt tacggctccg accgcgcggg ctacaactac ctgttcgcgc
     1081 gcggcagccg catcgccaac taccaaatca acggcatccc cgttgccgac gcgctggccg
     1141 atacgggcaa tgccaacacc gccgcctatg agcgcgtaga agtcgtgcgc ggcgtggcgg
     1201 ggctgctgga cggcacgggc gagccttccg ccaccgtcaa tctggtgcgc aaacgcccga
     1261 cccgcaagcc attgtttgaa gtccgcgccg aagcgggcaa ccgcaaacat ttcgggctgg
     1321 gcgcggacgt atcgggcagc ctgaacaccg aaggcacgct gcgcggccgc ctggtttcca
     1381 ccttcggacg cggcgactcg tggcggcagc gcgaacgtag ccgcgatgcc gaactctacg
     1441 gcattttgga atacgacatc gcaccgcaaa cccgcgtcca cgcaggcatg gactaccagc
     1501 aggcgaaaga aaccgccgac gcgccgctca gctacgccgt gtacgacagc caaggttatg
     1561 ccaccgcctt cggcccgaaa gacaaccccg ccacaaattg ggcgaacagc cgccaccgtg
     1621 cgctcaacct gttcgccggc atcgaacacc gcttcaacca agactggaaa ctcaaagccg
     1681 aatacgacta cacccgcagc cgcttccgcc agccctacgg cgtagcaggc gtgctttcca
     1741 tcgaccacaa caccgccgcc accgacctga ttcccggtta ttggcacgcc gacccgcgca
     1801 cccacagcgc cagcgtgtca ttgatcggca aataccgcct gttcggccgc gaacacgatt
     1861 taatcgcggg tatcaacggt tacaaatacg ccagcaacaa atacggcgaa cgcagcatca
     1921 tccccaacgc cattcccaac gcctacgaat tttcccgcac gggtgcctac ccgcagcctg
     1981 catcgtttgc ccaaaccatc ccgcaatacg gcaccaggcg gcaaatcggc ggctatctcg
     2041 ccacccgttt ccgcgccgcc gacaaccttt cgctgatact cggcggcaga tacagccgtt
     2101 accgcaccgg cagctacgac agccgcacac aaggcatgac ctatgtgtcc gccaaccgtt
     2161 tcacccccta cacaggcatc gtgttcgacc tgaccggcaa cctgtctctt tacggctcgt
     2221 acagcagcct gttcgtcccg caatcgcaaa aagacgaaca cggcagctac ctgaaacccg
     2281 taaccggcaa caatctggaa gccggcatca aaggcgaatg gcttgaaggc cgtctgaacg
     2341 catccgccgc cgtgtaccgc gcccgtaaaa acaacctcgc caccgcagca ggacgcgacc
     2401 cgagcggcaa cacctactac cgcgccgcca accaagccaa aacccacggc tgggaaatcg
     2461 aagtcggcgg ccgcatcacg cccgaatggc agatacaggc aggttacagc caaagcaaaa
     2521 cccgcgacca agacggcagc cgcctgaacc ccgacagcgt acccgaacgc agcttcaaac
     2581 tcttcactgc ctaccacttt gcccccgaag cccccagcgg ctggaccatc ggcgcaggcg
     2641 tgcgctggca gagcgaaacc cacaccgacc ctgccacgct ccgcatcccc aaccccgccg
     2701 ccaaagcccg cgccgccgac aacagccgcc aaaaagccta cgccgtcgcc gacatcatgg
     2761 cgcgttaccg cttcaatccg cgcgccgaac tgtcgctgaa cgtggacaat ctgttcaaca
     2821 aacactaccg cacccagccc gaccgccaca gctacggcgc actgcggaca gtgaacgcgg
     2881 cgtttaccta tcggtttaaa taagatcgtc tgaaaacgga gtttctgcga agctatagtg
     2941 g
//
LOCUS       DAXHNE010000187         2869 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.187, whole genome shotgun sequence.
ACCESSION   DAXHNE010000187 DAXHNE010000000
VERSION     DAXHNE010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.187"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(17..826)
                     /locus_tag="ACFM4Z_001941"
     CDS             complement(17..826)
                     /locus_tag="ACFM4Z_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFC6021917.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLPTMGVVRVYSSDIGTITHKFVEDGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLAEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRSRLESLKREQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSG"
     gene            complement(840..1010)
                     /locus_tag="ACFM4Z_001942"
     CDS             complement(840..1010)
                     /locus_tag="ACFM4Z_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021918.1"
                     /translation="MNTETIYATVFCILAATISGLLVKFNVIKIETSINSKFMLLGIS
                     ILIIGIFLSIFF"
     gene            complement(2051..2287)
                     /locus_tag="ACFM4Z_001943"
     CDS             complement(2051..2287)
                     /locus_tag="ACFM4Z_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HFC6021919.1"
                     /translation="MKELHTSELVEVSGGKFHIFAQGGGNLGKKDMVAVGKIGASYSP
                     NNSGVEFSVSKQFGYVQGLGVQFSKPTFGISKKW"
BASE COUNT          919 a          500 c          482 g          968 t
ORIGIN      
        1 tatttttgca aaggtctcaa cctgatttac tagctcgtat ggtttgttcg gatttcaaat
       61 caaaatccaa aatttcttgg ttcatttccg taatcgcacg gttgagttgg ctcaattcgg
      121 ttttatggcg ttcaggcagg ctgcttaatg tgattttctg ttcatcaagt tccctgattg
      181 cattattttg ttcacgtttt aggctctcca aacgtgagcg ttgttccaat aaatggcttt
      241 cggcggtcat cttatcttgt tgggatactg cgccttgact ggctaaaaac ttgttcttgt
      301 taagggtttt ttccgctaaa cgaatttgac gattttgccc tgtaatttgc tgtttaatat
      361 tctctaattg attgtttaaa cgatgaatgt tgttatgaac atttttttgc tcattttgat
      421 gaatgcgctt taaacgttcc aattcttgta atgccaaagt ttttttaagg ttggcttctg
      481 ctgccaattt ggcttgtacg tttccttttt cgccaaaacg cgatgtggaa agtttgaaca
      541 atggttcgcc agctttgaca aagttaccat cttcaacaaa tttatgcgta atcgtgccga
      601 tatcggaaga gtaaacacga accaccccca tagttggaag taattgacct tcaacggttg
      661 ttttattggt atagctacca aaaatcaaaa agataatgat acacagagca atgagaaaag
      721 cgcaaaaagt cagaaataaa aaagagaatg gacgggtcaa gataacctga cctgtccact
      781 tattttgttg ggctacaaag acttcttttc ggaataagtg ggacatttat tattatttct
      841 taaaaaaaaa tggatagaaa aataccaata attaaaatac ttatgcctaa taacataaat
      901 ttgctattga ttgatgtttc tattttaatt acattaaatt taaccaataa tccagaaatg
      961 gttgcagcta aaatgcaaaa gacagtggcg taaatggttt cagtattcat aatttttctc
     1021 tttcaaaatt gttcaatcta aatagaaaaa ggtaacttca atacatattg ctgtaactga
     1081 aattaccttt tccttaatga tgtaattatc ttatggcttc atcatgccac gaccgatatt
     1141 gtatccgcca gcaacagaga aaataccagc tcccattgcg tcaacccagc ctactggtcc
     1201 tgtccacttg ttccgttgag ctacaaagac ttctggtctg aaaaatgagt gattgttaga
     1261 catgtgtact tatttatgtt tctttgattg ggataaattt gtaaaaacaa aaaaaaggca
     1321 agttgttatt atagaagaac ttaaaatgct taacatactc attgaggttt gtaccacata
     1381 atgagatacg agataatttg ttgggagtaa aatcaaaaaa aagattaaat atttaaaata
     1441 atcactatat tttttcatta tttgttcctt tttgtattta agttcttatg aaaaactagg
     1501 gtattttgaa tagaaaatat caaaataccc tagttttatt aaggaatatt accaccaaca
     1561 agtgcttcca tattgtacag caccagtgat tgcaccacca atagctccga atttcgcacc
     1621 tggaatagca ccaataccac ctgcaaatga accaacaatt gccccaccag ctgctccacc
     1681 taatgcacta ccaatggtat tttttgagaa atcacgccag ttacaagcag caccagaaac
     1741 ttgttaaatt tcattcaaag tcaaaacttg cataatattc acctttgtta agtgtggttg
     1801 agaaaaaagt aaattttcat cgttactcat tatttgcaat catgcatata ctgtaaccaa
     1861 gcactatatt cagttggatc atagtttttc ctactgggtt gtctgatatg actattggca
     1921 ctattggcac aattgggatt agcaacttta ttgtgagtac ctaatggatt gatatttcct
     1981 gtaaagtggc tattgcatgc gcttaatgag catagattta ttaatgtcag aaaggataaa
     2041 acaaaaaatc ttaccatttt ttactaatac caaaagtagg tttcgaaaac tgtacaccaa
     2101 gaccttgtac atatccaaat tgcttgctaa cagaaaactc tactccacta ttgttagggg
     2161 aataggaagc accaatttta ccaacagcaa ccatatcttt tttacctagg ttgccgccac
     2221 cctgtgcaaa gatatggaat ttgccacctg acacttcaac taattcagag gtgtgtaact
     2281 ctttcatgat tttctcctgt aaagaagtat ggttggaaat gtaaatcaga attaaccttt
     2341 cttaagttta atttaatact ttttttgcaa gtattttatt ttaaattaaa tcaatatttt
     2401 aaaaatattt acttgtatac tgaaaaagaa actcctaatg gtggagtggg gcttttgttt
     2461 aagtaagcat taaaaaaatt taaatttccc attatgatta acttaatgca agagcttttt
     2521 caggacgtac aaaccagcga acgggcgttc tgatgtgggc agcctgccgg gaagtctgct
     2581 ctattgttcg gacaataggg ggaatatacc aattacttta actacatcat tttttgacat
     2641 ttttcttgcc tttcaattta aaatccaaaa tcatactgcc ataatttagc aatccaaaaa
     2701 aatttaggca gcagtaatgg tttcgtattt tagaaaacga aacttggttt ggtgttttga
     2761 agcatttcag tacacaaacg gggctgtcct agataactaa gataaactcg attttactaa
     2821 ttgttttaaa atggaacaag aacttttatc tcactgttgt taaaacgtc
//
LOCUS       DAXHNE010000188         2802 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.188, whole genome shotgun sequence.
ACCESSION   DAXHNE010000188 DAXHNE010000000
VERSION     DAXHNE010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.188"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..457
                     /locus_tag="ACFM4Z_001944"
     CDS             <1..457
                     /locus_tag="ACFM4Z_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFC6021920.1"
                     /translation="ISTINVDIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIKP
                     KDKVVLRYQAYPYQKFGHATGEIISVARTALGKQKLSGLGIIFTNPTLLNEPAYLVKV
                     KLEKQTIKAYGENKPLQIGMILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            460..2546
                     /locus_tag="ACFM4Z_001945"
                     /pseudo
     CDS             460..2546
                     /locus_tag="ACFM4Z_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
BASE COUNT          919 a          486 c          485 g          912 t
ORIGIN      
        1 tatatcaaca attaatgttg atatagggca acaagttgaa ccgtctaaat tgctgttaag
       61 cattgtccct gaacaaactg aattggtcgc caatctttac atacccagta aagctgttgg
      121 ttttattaaa ccgaaagata aagttgtttt acgttaccaa gcgtaccctt accaaaaatt
      181 tggacatgcc acaggagaaa ttatttcagt tgccagaact gctctcggta aacaaaagct
      241 atcaggttta ggtatcattt tcactaaccc aaccttatta aatgaacctg cctatcttgt
      301 gaaagttaaa ttggaaaaac aaacgattaa agcatacgga gaaaacaagc cgcttcaaat
      361 tggcatgatt ttagaagcag atattctcca tgaacgaaaa aaattgtacg aatgggtact
      421 tgacccactt tacagcattt caggaaaaat caattaaaaa tggattattt atcaagactg
      481 tcctttggat ttaacaaaaa gctacctgtc attctgcaaa cagaagttgc tgaatgtggt
      541 ttagcatgcc tgacatccat cttgtcctat tatggctttc acactgattt aagaacgtta
      601 cgccaaaaat acaccctgtc attaaagggc gcaaatcttg cagacatcat gagatttggc
      661 aatgaaatga atttaacgcc acgagctttg cgtttagagt tagatgagct gtcaaattta
      721 caactaccct gcattctcca ttggaactta aaccattttg ttgtactttg ttccatttcc
      781 aaagacagta tcgtcattat ggaccctgct gtcggtatgc gaaaaatcaa aatggacgaa
      841 gtttcacaaa aattcacagg gattgcccta gaattattcc ccaataccca ttttgaagag
      901 aaaaaagaaa caaagaaaat caaaatatta tctctattaa gggggggtca ggcttaaaac
      961 gctctttaat tcaaatgctt atattagcta tttctttgga agtctttgca ttggttagtc
     1021 cattctttat gcaatgggta atagaccatg tcattgtaac tgctgataaa aatttattat
     1081 tgacccttac tttgggattt ggtttactga ctatcctgca acagttaatt agcctgttac
     1141 aagcatgggt aggtatgcac ctatctacaa ctcttaattt acaatggaaa gccaatatat
     1201 ttaaaaggtt acttgactta cctaatgact atttcagtaa acgacattta ggagatgtga
     1261 tttcaagatt tggttcaata gatcatatcc aagaaacact aacttctact ttttttgttt
     1321 tagttttaaa tagcttaatg gctgttttta ctttcgtgtt aatgacaatt tacagcactc
     1381 aattatcgct gattgttctt ttaacacttg ttttgtacat actaattcgt tggcttgcat
     1441 attacccatt aagaaatgca acagaagaaa atattgttca tgaagccaaa caaaactcat
     1501 atttcatgga aaccattcgt ggtatccaat cagttaaatt atttgataaa cattatcaaa
     1561 gacatggcac ttggatgagc ctatttgtga atacagtcaa taccaagctg acaacagata
     1621 aactctctgc tttatttgaa ttttcaaata aactgttgtt tagcatggaa aatgttatca
     1681 taatttatct tggtgcaagc gcaattttag atggttcatt tacagtcggt gttctgatgg
     1741 cttttttggc ttataaaggg caatttgaaa gcagaacagc ttctctcgtt gaccaataca
     1801 tccaaatcaa aatgttaggg cttcatgctg aacgtttggc tgacattact ttaaatgaaa
     1861 cagaaactga aattattaag tataatcata tacctaaatt agataatgaa caactggttc
     1921 ttaaagttga aaacgtctca ttcagatatg ctgataatga gccatatctt tttgaaaaca
     1981 ttaatttgga atttaaagat aatgaagcag ttgttttaac aggacaatct ggtcggggga
     2041 agtccacttt gttaaacatt ttaacaggta gcctaaaacc tgaaactggt acagttagta
     2101 ttaatgggca tgatatatat caagtttctc catcctttat taggggattg agcgggattg
     2161 ttcgccaaga tgatgtcctt tttgcaggtt ctattgggga aaatatttca ttttttgatg
     2221 aaagcccaaa tatggagctc attgaacaat gtgcaaaaat ggcacaaata catgacgata
     2281 tacttaaaat gccaatgggc tatgagacct tgattggcga tatgggaaat atcttatcag
     2341 gtggacaaaa gcagagagtt atcttggctc gtgcattgta taaacgaccc aaaattctat
     2401 ttttagacga agcaagtagc catttagatg tagaaaatga acaaaaaatt aaccataacc
     2461 taaaaagtct tggtattatg aaaataatgg ttgcacaccg ccaagaaaca attcaatcgg
     2521 cagataaaat tctgaattta ggttgaacag aacaagactt catttttctt taacaaaaag
     2581 tgaagtcttt tttcaaataa tttaatagaa tacatgaaaa tagcggttta acgttccatt
     2641 tcccaatcat cacgactggc tttgtgtttt ggcgattttt cagtttcctt tttctgttga
     2701 atttgttgtt ttttctgctc ttgttcccat ttttgggcta atttcacggt ctcattttca
     2761 gcccattcca tcacggcaca acgatgtagc ttttctccga ta
//
LOCUS       DAXHNE010000189         2750 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.189, whole genome shotgun sequence.
ACCESSION   DAXHNE010000189 DAXHNE010000000
VERSION     DAXHNE010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.189"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..313
                     /locus_tag="ACFM4Z_001946"
     CDS             <1..313
                     /locus_tag="ACFM4Z_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6021921.1"
                     /translation="SEIWSNPKATFLDPVCKSGVFLREIVKRLDEGLTNQIPDKQTRI
                     NHILKNQVFGSTATYVGSFDRYLHKNSFAGERNYFANFKIFLAPNSTMTKTIMPMIKS
                     G"
     gene            complement(232..1032)
                     /locus_tag="ACFM4Z_001947"
     CDS             complement(232..1032)
                     /locus_tag="ACFM4Z_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HFC6021922.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(1168..1674)
                     /locus_tag="ACFM4Z_001948"
     CDS             complement(1168..1674)
                     /locus_tag="ACFM4Z_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021923.1"
                     /translation="MDFFDFFAQGKYKKIATEQGLDRKYFIFHYIVCNLSAISSFCLM
                     KVIHSYTILCLLIMIIGIAFGFILRNRVLSIVKNKQNFLSDMFPLEMPFFSRVYKIPI
                     LARFLNRGMKWLYLQNFFCLVMICFSISLILNEERGWMPYFICYWFGLCYPANTYYVF
                     LSRDYKHI"
     gene            complement(2011..2652)
                     /locus_tag="ACFM4Z_001949"
     CDS             complement(2011..2652)
                     /locus_tag="ACFM4Z_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HFC6021924.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
BASE COUNT          888 a          585 c          477 g          800 t
ORIGIN      
        1 gtctgagatt tggtcaaatc caaaggcgac attcttagac cctgtgtgta aatcaggggt
       61 atttttgcgt gaaatcgtca aacgcttgga tgaaggcttg accaatcaaa taccagataa
      121 acaaactcgc attaaccaca ttttaaaaaa tcaagttttt ggaagtactg ccacgtatgt
      181 aggtagcttt gaccgatatt tgcataaaaa ctcctttgct ggtgaaagga attattttgc
      241 caattttaaa atatttctgg caccaaatag tacaatgaca aagacaatca tgccaatgat
      301 taaatcagga tagctagaat gagtcaataa cgtcaatgct cccgccgcta tcacaccgat
      361 attgatgata atgtcattgg atgtaaaaat catgctggct ttgatatgga tttctttatt
      421 ttgatttttg ctcagtagat ataagcacag ccagtttgca atcaatgcca aaaatgccgt
      481 gccaatcatc agttgataat tgggcagctg ctcagcaccg ataaaacgcc taatcacttc
      541 tatcacccca aataacgcca atattatctg cgttatcccc gccaaaaatg ccacacgttt
      601 tttatacgcc agcgtcatac caatggctga tagcgccaat atatagacaa agctgtccgc
      661 cagcatatct agactatcag caatcagccc catagaatta gcaaaaatac caaccgaaca
      721 ctctatgata aaaaacacaa agttaatcat gagcacttga tataataatc ttttttctaa
      781 gtgctcatca ggcttgttaa acactatctt atcaacaatc acttcggtgg aaatgatatg
      841 actatcaaaa ttaagcggtt caagtacttg taaaatcgtt gtatcttgat tatcgtgata
      901 gacggttaag caccgcccag caatatcaaa ctgtaattca taaatatcag acacatcttt
      961 taaacgcatg cgaatgagct gttcttcgga cgggcagtcc attttggtaa tgttaaaaat
     1021 ggtctttttc atctatttag ttccttgttt tgatcaggtt ggctcaaata aatctgtgtt
     1081 tatattgctg cttggtaatt tttggatggt ttgagtaaat tgattaggtt aaaatttacc
     1141 tttggaagta ccgccacgca taatagttta gatatgttta taatctctgg ataaaaaaac
     1201 gtaataagtg tttgctggat aacaaagtcc aaaccaatag cagataaaat aaggcatcca
     1261 ccccctttct tcattaagga taagagatat cgagaaacaa atcataacta aacagaaaaa
     1321 attttggaga taaagccatt tcattcccct attcaagaat ctggccaaga taggtatttt
     1381 gtatactcta gaaaagaaag gcatttccaa gggaaacata tcagataaaa aattttgttt
     1441 attttttact atagatagaa ccctatttct caagataaat ccaaatgcaa tgccaattat
     1501 cattattaat aaacaaagga tagtataact atggataacc ttcattaaac aaaaagaaga
     1561 aatggcagat aaattacata cgatatagtg gaatataaaa tatttacggt ctaaaccttg
     1621 ttcagttgca atttttttat acttaccttg cgcaaaaaaa tcaaagaaat ccattaaact
     1681 atctttcaca ttagaaattt aaaagctaaa taatacgaca aacaatgtga agtactattc
     1741 atggtttatt taaaaaataa tactattctg aacattattt agatacagaa attaacaaat
     1801 tagaactaaa caagctttta aatactttaa ttttattgga aagctataaa aggaactata
     1861 actttacaca ctagtcactt ctttttaaga ggcaaaaggg attgggaagg tcgtcttgga
     1921 gataagcact ggtatttcgg ccaatggtaa atagagttta cctcaaatag ggtagaacct
     1981 ccttcatctg tcagttaata acagccactt ttacaatgcc ctgtcgaaat aaagtggcac
     2041 gcccgatttt tcactcatac cgattaaata atccatcgtc ttccgaggga acgcgatgcc
     2101 gcgcaccacg gtcagattcc tcaaaacggg gaaaaccaaa atatcctcca taccgattcc
     2161 gccgttgatg ccgtctgaag caccgtccat caaattttcc aactcttgca aatctgcgtt
     2221 tatccgttcg aggtattggg cggttttatt caaattggcg gaaaagctgc cgatgctttt
     2281 ctcttttttg tctgtaaaat atttcaccgc ttccggcgtt gcaaattcag gcagcccgat
     2341 tttgatcacg cgcggctgca ccagtttgtc gttgtatccg cccaccttgt ccagccacgc
     2401 ccgtatctcg gggcggactt cgtctttcag acggtcttcg cggtcgaaat gccgcacaat
     2461 gtccaaactc tcgcccataa acgaaccgtc ttctttttgc aggacgggca cttgtttcgc
     2521 accgatcata ccgatcggcg ttgcctcgtc gtcgtttgcc agcacggctt cttcaacgtc
     2581 cgcgccaaac agcccggcag ccatccgcgc acgcacgcaa aacgggcaat ggtcgtaaat
     2641 atacagtttc atcaaaatat tcctcgtcaa cctgtcggta ccggctacct taacaccccg
     2701 cgccacccga aacaagttta tcttcccgcc tatgcaccgt aaataaataa
//
LOCUS       DAXHNE010000190         2628 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.190, whole genome shotgun sequence.
ACCESSION   DAXHNE010000190 DAXHNE010000000
VERSION     DAXHNE010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.190"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            121..735
                     /locus_tag="ACFM4Z_001950"
     CDS             121..735
                     /locus_tag="ACFM4Z_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFC6021925.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNSANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGVYILIENNSFDMLWTVLG"
     gene            896..2557
                     /locus_tag="ACFM4Z_001951"
     CDS             896..2557
                     /locus_tag="ACFM4Z_001951"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HFC6021926.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPDE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADSYDAARFA
                     GVFFAVIGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPVAHFLNPAAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDYHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIGIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVLLPQNADAPQGWQTVWQGARPRNKDSKFALIRKIGENI"
BASE COUNT          515 a          664 c          682 g          767 t
ORIGIN      
        1 aaggcgaggc aacgccgtac cggtttttgt taatccacta tattttgacg gggttgaagt
       61 tgacatctac aaggtttaat gtttgaatac attcaaaaat atatttgaat gagatgtttt
      121 atgattcaaa atgtcgttac ttcaataatc ctgtattctg ggacagccgt agacttactt
      181 attatcctaa tgttattttt tgccaaaaga aaaagcagaa aagacatcat taacatctat
      241 ttaggacaat ttctaggctc tgttagcctg atattgctaa gtttgctttt tgcatttgtc
      301 ttagattata ttcctagtaa agagatttta ggtttgctcg gcttgattcc aattttccta
      361 ggcatcaaag ttttgctgtt aggagattct gatggagagg ctattgccaa agagggtttg
      421 cgcaaagata ataaaaacct gatttttcta gtcgctatga ttacttttgc aagttgtggt
      481 gctgacaata ttggtgtctt tgtcccatat tttactacct taaattcagc gaatttgata
      541 gtggctttac ttacctttct agtcatgatt tatctcttgg ttttttctgc ccaaaaattg
      601 gcacaagtcc cttctgtcgg agaaactttg gaaaaatata gcagatggtt tgttgctgtt
      661 gtttatttag gattgggggt atatatcctg attgaaaaca acagttttga tatgctatgg
      721 actgtgttgg gctaggaaaa aatattatga aaaagatagt atctgcaaag actgccatgt
      781 gcagtctttt ttgttgccgg tctttttgtg tctgatgccg tctgaagcag tctctgcacg
      841 acctttgtgc gaatatttgc tacacttggc aatccgtcca ttttttctca cgcttatgct
      901 gacctatacc ccgcccgatg cccgcccgcc cgccaaaacc cacgaaaagc cgtggctgct
      961 gctgttgatg gcgtttgcct ggttgtggcc cggcgtgttt tcccacgatt tgtggaatcc
     1021 tgacgaacct gccgtctata ccgccgtcga agcactggca ggcagcccca cccccttggt
     1081 tgcccatctg ttcggtcaaa ccgatttcgg cataccgccc gtgtatcttt gggttgccgc
     1141 cgcgttcaaa catttgctgt cgccgtgggc tgccgactca tacgatgccg cacgctttgc
     1201 aggcgtattt tttgccgtta tcggactgac ttcctgcggc tttgccggtt tcaacttttt
     1261 gggcagacac cacgggcgca gcgtcgtcct gattctcatc ggctgtatcg ggctgattcc
     1321 agttgcccat ttcctcaacc ccgctgccgc cgcctttgcc gccgccggac tggtgctgca
     1381 cggttattct ttggctcgcc ggcgcgtgat tgccgcctct tttctgctcg gtacgggctg
     1441 gacgctgatg tcgttggcag cagcttatcc ggcagcattt gccctgatgc tgcccttgcc
     1501 cgtactgatg tttttccgtc cgtggcaaag caggcgtttg atgttgacgg cagtcgcctc
     1561 acttgccttt gccctgccgc ttatgaccgt ttacccgctg ctcttggcaa aaacgcagcc
     1621 cgcgctgttc gcgcaatggc tcgactatca cgttttcggt acgttcggcg gcgtgcggca
     1681 cgttcagacg gcattcagtt tgttttacta tctgaaaaac ctgctttggt ttgcattgcc
     1741 cgcgctgccg ctggcggttt ggacggtttg ccgcacgcgc ctgttttcga ccgactgggg
     1801 gattttgggc gtcgtctgga tgcttgccgt tttggtgctg cttgccgtca atccgcagcg
     1861 ttttcaggat aacctcgtct ggctgcttcc gccgcttgcc ctgttcggcg cggcgcaact
     1921 ggacagcctg aggcgcggcg cggcggcgtt tgtcaactgg ttcggcatta tggcgttcgg
     1981 actgtttgcc gtgttcctgt ggacgggctt tttcgccatg aattacggct ggcccgccaa
     2041 gcttgccgaa cgcgccgcct atttcagccc gtattatgtt cctgatatcg atcccattcc
     2101 gatggcggtt gccgtactgt tcacaccctt gtggctgtgg gcgattaccc ggaaaaacat
     2161 acgcggcagg caggcggtta ccaactgggc ggcaggcgtt accctgacct gggctttgct
     2221 gatgacgctg ttcctgccgt ggctggacgc ggcgaaaagc cacgcgccgg tcgtccggag
     2281 tatggaggca tcgctttccc cggaattgaa acgggagctt tcagacggca tcgagtgtat
     2341 cggcataggc ggcggcgacc tgcacacgcg gattgtttgg acgcagtacg gcacattgcc
     2401 gcaccgcgtc ggcgatgtac aatgccgcta ccgcatcgtc ctcctgcccc aaaatgcgga
     2461 tgcgccgcaa ggctggcaga cggtttggca gggtgcgcgt ccgcgcaaca aagacagtaa
     2521 gttcgcactg atacggaaaa tcggggaaaa tatataaaaa acaacagatt gagccgaatt
     2581 tctggattaa gtgccggaaa tcgcgtataa ttgcgcgatt aaaccttt
//
LOCUS       DAXHNE010000191         2614 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.191, whole genome shotgun sequence.
ACCESSION   DAXHNE010000191 DAXHNE010000000
VERSION     DAXHNE010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.191"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(313..546)
                     /locus_tag="ACFM4Z_001952"
     CDS             complement(313..546)
                     /locus_tag="ACFM4Z_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFC6021927.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(712..912)
                     /locus_tag="ACFM4Z_001953"
     CDS             complement(712..912)
                     /locus_tag="ACFM4Z_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFC6021928.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(1084..1959)
                     /gene="xerD"
                     /locus_tag="ACFM4Z_001954"
     CDS             complement(1084..1959)
                     /gene="xerD"
                     /locus_tag="ACFM4Z_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFC6021929.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLATPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(2011..2451)
                     /locus_tag="ACFM4Z_001955"
     CDS             complement(2011..2451)
                     /locus_tag="ACFM4Z_001955"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFC6021930.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
BASE COUNT          703 a          741 c          521 g          649 t
ORIGIN      
        1 ctaccgatta cacaaacaca tatcatcttt acacaatcat gcttccatca acagtaaaac
       61 atgatatgat tgccaacaat aacatctcac aataaatttt ctaattttta ttgaaaaaat
      121 caataaataa gaattctccc aaccgacaaa taaaataaag aaagggttaa tatgcaacat
      181 cgaagaagac tagcaattta ccaagcatcc aaacgtgctt cctttaccgg caggtcatcc
      241 gccccgcaaa aacgtaaaga acgttgattt gaaaaaaatg ccgcctgaag tcctgcttca
      301 gacggcattt ttttaccgtt cgagaaactg tttcaacctg tcctcatcca aaaaccagtg
      361 acagatttcc tcatctcccg ccaacaaaac gggaaccagc tcattgtatt tttcttccaa
      421 aacaggattt tcatccacat cgaccacttc cagcccgaac ccgtattcat tttgaaaagg
      481 tttgagtgca tcgcgcattt tgtggcacaa gctgcaatat tcacgaaaca tcaaggtcaa
      541 tttcatccgc gtttccttat ctgtcaattt gcacacgcca aagccttaga cgcagcagaa
      601 tcatggtcta tttgggaaaa aacaatgttt tcaaggaaga tgatactcaa gttctgccaa
      661 aaacagcaaa aatgccgtct gaacagttca gacggcattt cgaaaaccgt tttacgcttg
      721 aacgttgata cccgccgtaa ccgtcggttg cgcattgtgc gccgtcagaa acgaagcggc
      781 aagctccccg cagcagccgc agcagctcgc tacgcccaat tgcgactgca aatcgcccat
      841 tgtggtcgcg ccggcggcga tggtttcctt gatttgatgg tcggtaacgg cattgcagat
      901 gcagacaaac attttgtgct ccgtgtgttc tcaaactatc gtaccgatag cggcttttat
      961 tatcgtatgc aaatataaat aaaaacggtt cgcattgcaa ggtcggcata cacggtttat
     1021 cttggtaatt ttttatcaag tttgtatttt cgattgattt cttattaaac cgaagcagaa
     1081 gcctcagttt cgggaatggt gttccttcac tacaccctgc aaccatacgt tggcaacatg
     1141 ggtataaatc tgcgtcgtat tcaaatcggc atgtcccaac atatcctgaa ccacgcgcaa
     1201 atccaagccg tgccgcacca gatgcgtggc aaaggcgtgg cgcaggctgt gcgggctgat
     1261 gtgcccgatg cctgcctgac ttgcatattc tttgacaatc atccatgcca actgacgaga
     1321 aatgcccgtc tttttctgac tgacaaacaa tgcgtcgcaa ttcctgcctt tcagcagaag
     1381 tgggcgtgcc tccgtataat agcgttccac ccaatacgcc gactcctgcc ccatcgggac
     1441 catcctctgc ttatcaccct ttcccagcgc ggtaatacag cccctgtcca aatccacatt
     1501 gccgaagttc agcccgaccg cctcgctgac gcgcaagccg gtcgcgtaca tcaattcgag
     1561 cagtgccttg tcccgcaaac cgtgtggcgt atcagtatcc ggagtggcaa gcagccggga
     1621 aatctgctgt tctgtaatca gtgtcggaat attcttgtcg attttgggcg gtttcagcaa
     1681 acgggtggga ttgtccgtcc ttatgccttc acgctccatc catatataca ggcgtttgca
     1741 cgccgataat gcgcgcgcct gcgaactccg ttgctctccg tcaacataaa ccgccgccgc
     1801 caaatccgct tcgtccgcat ccttcagcat tctgcccgat tgggacaggc ggcgggcgat
     1861 tttttccaaa tcgcgccggt aaccgtttaa agtattctga ctgagccgcc tgtccaacca
     1921 cagcgtttca agcagcctgt cgatcaaacc ttcttccata ccgttccaaa caaatgccgt
     1981 ctgaatcttc ttcagacggc atggttcaca ttatcgggaa agcgtttcca atacttcctg
     2041 cgcgtgaccc gccactttga ctttccgcca ttcatgggcg atttctccat ccttattcaa
     2101 gacgaacgta ctgcgctcga tacctaacga ctctttcccg tacagtttct tcaatttgat
     2161 gacatcaaac aggcggcaca ctgtttcatc cttgtcgctc aacagctcga accggaaacc
     2221 ctgcttggcg caaaaattct gatgcgcctt tacgccgtcg cgggaaatac cgaccacggt
     2281 ataacccaat gcctcaaact gttccaaacg cgcattgaaa tccaagcctt ccgtcgtaca
     2341 gccaggcgta ctgtctttcg gataaaaata cacgaccaaa ggcagatgtt ctgccgaatg
     2401 aaaatccgca ccgctgctcg aaggcagggt aaattcatat ttcacatcca tagtcctact
     2461 cccgatattc ccattattca aaacggcacg cagaggcacc gccgcaattt ccaaaccaac
     2521 cccgattcta ccgccccaaa ggacaaggat tcaaccgccg gaaacatcca aaccgacaca
     2581 cgacggcatg aaaaatatcc atgtcaaacc acaa
//
LOCUS       DAXHNE010000192         2596 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.192, whole genome shotgun sequence.
ACCESSION   DAXHNE010000192 DAXHNE010000000
VERSION     DAXHNE010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.192"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(213..284)
                     /locus_tag="ACFM4Z_001956"
     tRNA            complement(213..284)
                     /locus_tag="ACFM4Z_001956"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(249..251),aa:Arg,seq:cct)
     gene            complement(304..750)
                     /locus_tag="ACFM4Z_001957"
     CDS             complement(304..750)
                     /locus_tag="ACFM4Z_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFC6021931.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(754..1728)
                     /gene="ispB"
                     /locus_tag="ACFM4Z_001958"
     CDS             complement(754..1728)
                     /gene="ispB"
                     /locus_tag="ACFM4Z_001958"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFC6021932.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVAALDALPDSEVKDAMIQLAKESLVRVS"
     gene            1952..2260
                     /gene="rplU"
                     /locus_tag="ACFM4Z_001959"
     CDS             1952..2260
                     /gene="rplU"
                     /locus_tag="ACFM4Z_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFC6021933.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2285..2557
                     /gene="rpmA"
                     /locus_tag="ACFM4Z_001960"
     CDS             2285..2557
                     /gene="rpmA"
                     /locus_tag="ACFM4Z_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFC6021934.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
BASE COUNT          762 a          696 c          576 g          562 t
ORIGIN      
        1 aatttaatcc actatatcat aaaacaacag atttccaagc cggaacatct tttacggagc
       61 ctgaaaatca tttcatgatt ctaccgtcct aaaggtcggt ttttcaagca ggaagaaaaa
      121 gttttcagat ggcaaaaaag ccctccaaca ctgaaaggcc ttatatcgga aacttcccgc
      181 aacacgggaa acagacaaat gaaatcgtca aacctcgcca acaggaatcg aacctgtatt
      241 ttacgcttag gaggcatacg ttctatccgt tgaactatgg cgagccgaaa tgaaaaggag
      301 attttaaccc tttccgacga caaaagacaa gatgccggcc gcaatcagcg gcccgacagg
      361 gataccgccc ataaatgcca ccccgataac cgtcccgatt aacagccctg taattaaaac
      421 aggctgctgt cccatcaaag gcacgccgcg tcccgccagc caagccacga aaataccgat
      481 aaaaacggcg gatatcattt taaaattcaa aaattcggca acgggaggaa cctgcgcctt
      541 tcctgaaacc aacggactca aaacccctat ggtcaaaaga atgataccga gattcaaccc
      601 gtgcttctcg accaacggga caaactgtac caatgccgtc tgctgcatca gcagcaatat
      661 ggttgccgag atggtaatcg aattgttgtt gctgaccacc cccaacagaa tcagcgtaac
      721 caaaaacagg ggaacaaaac tgaaattcat cggttacgac accctgacca aagattcctt
      781 cgccagctga atcatggcat ccttcacttc gctgtcgggc agggcatcca aggcggcaac
      841 ggcacaatcg actgctttgc gcgcctcgcc tattgaatat gccaacgcat ccgaacggac
      901 gacataatcg tggattttct caaaatagct gcgatctgca ttttccaaag cagtacgcac
      961 atcgttcgca acctgttcgg aaccctgacg catcagataa atcaaaggca aagtcggttt
     1021 tccttccgcc aaatcgtcgc cgacgttttt gccggtttct tcggtttcgc cagaatagtc
     1081 cagcacatcg tcaataattt ggaatgccgt accgacatac ataccgtagt ctttcaacgc
     1141 ccgttcgtgt tcgggggaag ccttgcccaa aattgcgccg acttgagcgg cagcttcaaa
     1201 caattttgcc gttttatatt ggatgacttg gatatattgt tcttcggtaa tgtccgtatt
     1261 gccgatgttc atcagctgca tgacttcgcc ctcggcaata atgttggttg catccgccat
     1321 cacttccaaa acgcgcatac tgcccgaggc aaccatcagt tgaaaggcgc gcgtgtataa
     1381 aaagtcgcca accaacacag ccgccgcatt gccgaacaga ttgtttgccg ttgcccgccc
     1441 acggcgcaaa tcgctttcat cgacgacatc gtcgtgcagg agggtggaag tgtggataaa
     1501 ctcgaccatc gccgccagcg aatacagttt ctcgtcatca taaccgaccg ccttacccgc
     1561 caaaatcgtc ataatcggac gcaggcgttt gccgcccgcg ctgatgatat atgtaccgat
     1621 ttgcgaaatc agtgcgacat cggattgcac cgcacggttg atgacttcat tgactttggc
     1681 aaggtcttca ggcagatgtc gctggaaata gggcagattc tcgagcatgg cagactctca
     1741 ggtttacgtt tccgtcaaac ggcagatacc gcttgaaagc tgattcaaat agcgggcaat
     1801 tatatcagca aagccatcag ggtacatctt ttcagacggc atatgattgt ccgaaccgcg
     1861 caaactttga caaaacaggc aaataacaat acaatgccga gttccgcaca atggtgtgcg
     1921 gatattaacc ataaccctta tggagttgag tatgtacgcg gtcgtaaaaa ccggcggcaa
     1981 acagtataaa gtttccgtcg gcgaaaaatt gaaagtagaa cagataccag ccgaactcga
     2041 cagccaaatc gaactgaccg aagttttgat gattgctgac ggcgaatctg taaaagttgg
     2101 cgcacccttt atcgaaggtg caaaagtaac ggctaaagta gtggcacacg gtcgtggcga
     2161 aaaagtccgc atcttcaaaa tgcgccgccg caaacactac caaaaacgcc aaggccaccg
     2221 ccaaaatttc acccaaatcg aaatcgtggc aatcgcctaa ccttaaaact taggagcatt
     2281 acaaatggca agtaaaaaag caggcggcag cacccgcaac ggtcgcgatt cagaagccaa
     2341 acgcttgggc gttaaagcct acggcaacga gctgattccc gcaggttcca tcatcgtacg
     2401 ccaacgcggt accaaattcc acgcaggcga caacgtaggt atgggcaaag accacacttt
     2461 gttcgccaaa gttgacggtt atgtcgaatt caaaaccaaa ggcgcgctga accgtaaaac
     2521 tgtcagcatc cgtccttaca ccggttctga agaataatcc aaccgatacc tgaagccgca
     2581 tcttttcacg atgcgg
//
LOCUS       DAXHNE010000193         2565 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.193, whole genome shotgun sequence.
ACCESSION   DAXHNE010000193 DAXHNE010000000
VERSION     DAXHNE010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.193"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..237
                     /locus_tag="ACFM4Z_001961"
     CDS             <1..237
                     /locus_tag="ACFM4Z_001961"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021935.1"
                     /translation="NLNHYKINFTENMPPHIRHSTSDRHQTRENHFPLPFTLPRSNTP
                     RSNKARTKPPKYAIIAAHIPRAMCSKRKNQPTRE"
     gene            242..2467
                     /locus_tag="ACFM4Z_001962"
     CDS             242..2467
                     /locus_tag="ACFM4Z_001962"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFC6021936.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFTRHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVTVPEATSVSIVFTDKQGNKKELRAPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGDKLAAAGVNVNNGFGNLLANLDKLDADTRTAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKDGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALNA
                     L"
BASE COUNT          707 a          783 c          590 g          485 t
ORIGIN      
        1 aatttaaacc actataaaat caatttcacc gaaaatatgc cgccgcacat caggcacagc
       61 acctccgacc gccaccaaac gcgggaaaac catttccccc ttcctttcac gctgccgcgt
      121 tcaaacacgc caagatcgaa caaagcgcga acaaagccgc caaaatatgc cataatagcc
      181 gcgcacattc cccgcgcaat gtgttccaaa agaaaaaacc aacctacaag agaataatcc
      241 tatgactcaa aaatccacca ttgtttatac ccataccgac gaagcccccg cgctggcgac
      301 ccaatcgctg ctgccgattg tgcaggcttt tacccgccac gccgatattg atgtcaaaac
      361 tgccgacatt tccctgtccg gccgtatttt ggcggcattc cccgaatatc tgaccgaagc
      421 gcaacgcgta cccgacgcgc ttgccgaatt gggcgaactg gtaaaacaac ccgatgcaaa
      481 cgtaatcaaa ctgccgaaca tcagcgcatc cgtaccgcaa ctgaccgccg cgattaaaga
      541 attgcagtct aaaggctttg ccgttcccga ttatcctgcc gacccgcaaa ccgatgaaga
      601 aaaagccgtg cgcgaacgct acgaccgcat caaaggcagc gcggtaaacc ctgtcctgcg
      661 tgaaggcaac tccgaccgtc gcgcccccaa agcggtgaaa aactttgcca aaaaaaatcc
      721 gcacagcatg ggcgcatgga caaaagactc caaaacccac gttgccacca tgcaaagcgg
      781 cgactttttc cataacgaac aatccgttac cgtacctgaa gcgacttccg tatccatcgt
      841 gttcaccgac aaacaaggca ataaaaaaga gctgcgcgcg cctgttgccc tgaaagccgg
      901 cgaaatcatc gacgcgaccg tgatgagtaa aaaagccctg ctcgccttcc ttgccgaaca
      961 agtaaaagat gcgaaagcaa aaggcgtgtt gttctcgctg cacatgaaag ccactatgat
     1021 gaaagtgtcc gacccgatta tcttcggaca cgccgtcaaa gtattcttcg cgcctgtttt
     1081 tgaaaaattc ggcgacaaac tggctgctgc cggcgtcaac gttaacaacg gcttcggcaa
     1141 cctgcttgcc aatctggaca aactggatgc ggacacccgc accgccgtcg aagccgaaat
     1201 cgccgccgtt tacgccgcca accccgattt ggcgatggtc gattccgata aaggcattac
     1261 caacctgcac gttcccagcg atgtcatcgt tgatgcttct atgcctgcga tgattcgtaa
     1321 ctccggccgt atgtgggata aagacggcaa agcgcaagac accaaagccg tgattcccga
     1381 cagcagctac gccggcgttt accaagcaac catcgacttc tgccgcgaac acggcgcatt
     1441 tgacccgaca accatgggta ctgtgcccaa cgtcggactg atggcgcaag cggcggaaga
     1501 atacggctcg cacaacaaaa cctttgaaat cgaagccgac ggccaagtcc aagtcattga
     1561 tgcggcagga aaagtcctaa tgcagcacga cgttgaagcc ggcgacatct ggcgtatgtg
     1621 ccaaaccaaa gacgctccgg ttaaagactg ggtacaactt gccgtcaacc gcgcccgtct
     1681 gagcaacacg cctgccgtgt tctggctcga cgaaaaccgt ccgcacgaca agagcctgct
     1741 cgccaaggtt aaagcctacc ttgccgaact ggataccaat ggcctcgaca tccgcgtcct
     1801 cgctcctgaa gaagccgcca agttcagctt gggtcgtctg aaaaacggcg aagacaccat
     1861 ctccgtaacc ggtaatgtct tgcgcgacta cctgaccgac ttgttcccaa ttttggaact
     1921 cggcacaagc gcgaaaatgc tgtctatcgt tccattgatg aacggcggcg gtatgtttga
     1981 aaccggcgcg ggcggttctg caccgaaaca tgttcaacaa ttcctcgaag aaaaccattt
     2041 gcgctgggac tcgctgggcg aattcctcgc actcgccgta tcgtttgaac atttggcgca
     2101 aaaaaccggc aatgccaaag cccaagtcct cgccgacact ttggatgcag ccaccgaaaa
     2161 actgctgttg aacgacaaat cgcctaaacg taaagccggc gaactcgaca accgcggcag
     2221 ccatttctac ctcaccctct actgggcgca agaattggcg gcgcaagaca aagatgccga
     2281 actgaaagcc gcatttacgc cattggcagc cgctttgacc gccgacgaag cgaaaatcgt
     2341 tgccgagctt tccgccgtac aaggcaaagc ggccgacatc ggcggctact acgccgccaa
     2401 tcctgaaaaa gccgcacaag tgatgcgtcc gagcgtaacc tttaatcagg cactaaacgc
     2461 cttataagca caaaggtaaa aatgccgtct gaacattcgt cgttcagacg gcattagtta
     2521 tccctatctg cctgattatg attaaacgcc gcacccattg gatat
//
LOCUS       DAXHNE010000194         2545 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.194, whole genome shotgun sequence.
ACCESSION   DAXHNE010000194 DAXHNE010000000
VERSION     DAXHNE010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.194"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            373..885
                     /locus_tag="ACFM4Z_001963"
     CDS             373..885
                     /locus_tag="ACFM4Z_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFC6021937.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            965..2044
                     /gene="aroB"
                     /locus_tag="ACFM4Z_001964"
     CDS             965..2044
                     /gene="aroB"
                     /locus_tag="ACFM4Z_001964"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFC6021938.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGALGDIGFFEWLEQHMPELMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT          626 a          772 c          608 g          539 t
ORIGIN      
        1 ttcagcacct tagagaatcg ttctctttga gccaaggcga ggcaacgccg tactggtttt
       61 tgttaatcca ctatagtatt gataaacata ttatcttcaa tatattcaat tggataattg
      121 tttacctaag caaagataat tgccttttcc tgacaaataa gtgaaatcaa cggattgtca
      181 aaacacagcc tgaaataaaa aacctccctg atttctttta tttgtcctta aaatcagaaa
      241 ggttcgggat ggtcgggtta tttttccaaa cgtaccgccg ccctgccgat ttcgtataaa
      301 attccgccgt aacccgacaa gcccgaaccc tgtcgccccg aaaggcgggg tgtcaaacat
      361 taaggaattg tgatgaaaaa ctttaacggc aaactcatcc tcatcggact gatgggcgcg
      421 ggcaaaacca cgctgggccg gcaaatggcg cagcggctgg attaccgttt ttacgacagc
      481 gatcacgaaa tcgccgcagc cgccggcgtt cccatcccca ccatatttga aatggaaggc
      541 gaacagggat tccgttcgcg cgaaaccgcc atactcaaaa agctggttat cctgccccat
      601 atcgtcctgt ccaccggcgg cggcgcggtg ttaaaagaag aaaaccgcgc ccttatccgc
      661 aaaagcggca cggtcgtcta tctgcacgcc ccgcccgaaa ccctgctcga acgcacgcgc
      721 tgcgacaaca gccgtccttt gctgcaagtt gccgatcctt tggcgaaatt acgtgaactc
      781 tacgccgcac gcgaccccgt ttaccgccaa accgccgact ttaccgtaga atcggcaaac
      841 tgccgggaaa ccgtgcaaac cctgctcaaa cgcttatccc gataaaccgg catatgcgcc
      901 gcgcccagaa aaccaaaccg cgccccgccc ggcgggccgg cggttcaaac tttaaggaac
      961 aacaatgaaa acactgaccg tacacacccc gtcccacagc taccccatct ttatcggcaa
     1021 cggactgctg ccgcaggcag gaagcctgct caaaccgcat ttgggcaaac gcgccgccat
     1081 catcgccaac gaaaccgtcg ccccgctcta cctcggcacg cttcagacgg cattggatgc
     1141 ggcaggcgta tcccatttca gcatcatcct gcccgacggc gaggcgcaca aaaactggca
     1201 gacgctcaac ctcatctttg acgggctgat gcaaaaccgc gccgaacgca aaaccacatt
     1261 aatcgcactg ggcggcggcg tgatcggcga catggtcggc tttgccgctg ccacctacca
     1321 gcgcggcgca ccgttcgttc aaataccgac cacgctgttg agtcaggtcg actcatcggt
     1381 gggcggcaaa accgccatca accacccgct cggcaaaaat atgattggcg cgttttacca
     1441 gccgcaggcg gtgcttgccg acttggacac gctgcacacc ctgcccgccc gcgaattgtc
     1501 cgccggtatg gcggaagtca tcaaatacgg cgcgctcggc gacatcggct tttttgaatg
     1561 gctggaacag catatgcccg aactgatgac gctcgatcgg gaaaaactcg cccaagccgt
     1621 gtaccgctgc tgccaaatga aggcagacat cgtcgcccaa gacgaaaccg aacagggcat
     1681 acgcgcatgg ctcaacctcg gacacacctt cggacacgcc attgaaaccg agatgggtta
     1741 cggcacttgg ctgcatggag aagccatcgc cgccggctgc gtgttggcgg cgcgtttgtc
     1801 cgaacaactg ggcaaaacct ccgccgcaga taccgcgcgg ctcgccgccc tgctcgaagc
     1861 cgccggactg ccgtccgcgc cacccgtgtt tgcctttgaa aaatggctgg aacacatgag
     1921 ccacgataaa aaagtcagcg gcggcatcat gcgctttatc ggtctgaacc ggctgggcga
     1981 agccaacatc accgaaatta ccgacacgga catcctccgc cgcaccctgc aaccgtatct
     2041 ctgatttcct ctgccgatgt gctgccgcgc gggtttgacg cacgatgatg attttccatc
     2101 atctttctcc gcaaaagcgg gaatccagtc cgttcggttt cggtcgtttc cgataagttc
     2161 ccgttgcttt tcatttctag attcccactt tcgcaggaat gacggtggaa aggtttttgt
     2221 tgtttcggag aagtttctgc aaccctagaa tctcgttatt tccacaaaaa acagaaaccc
     2281 gaaacagcaa cttaaaacct cgtcattccc gcaaaagcgg gaatccagtc cgttcggttt
     2341 cggtcgtttc cgataaattc ccgttgcttt tcatttctag attcccactt tcgtgggaat
     2401 gacggcggag aggtttttgt tgtttcggag aagtttctgc aaccctagaa tctcgttatt
     2461 tccacaaaaa acagaaaacc aaaacagcaa cttaaaacct cgtcattccc gcaaaagcgg
     2521 gaatccggtc cgttcggttt cggtc
//
LOCUS       DAXHNE010000195         2516 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.195, whole genome shotgun sequence.
ACCESSION   DAXHNE010000195 DAXHNE010000000
VERSION     DAXHNE010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.195"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            185..310
                     /locus_tag="ACFM4Z_001965"
     CDS             185..310
                     /locus_tag="ACFM4Z_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021939.1"
                     /translation="MNKTWKRQVFRHTALYTAILMFSHTGGGGGKRKRKRINTLL"
     gene            283..2516
                     /gene="pilC"
                     /locus_tag="ACFM4Z_001966"
                     /pseudo
     CDS             283..2516
                     /gene="pilC"
                     /locus_tag="ACFM4Z_001966"
                     /inference="COORDINATES: protein motif:HMM:NF040838.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
BASE COUNT          746 a          618 c          634 g          518 t
ORIGIN      
        1 agactgttgg gcatctgcag cggtttgcta aaaaacgctt taccgtgatc agtgtgcaaa
       61 gttaaaatgg ggaggtaagc ttttcaatca gcaattcggc gggcgcggaa tcgggcggtt
      121 taccgaaccc cggcgttcgc ggcgcgccgc cccgtcccgt gaaggcaaac tcaaggaata
      181 aaagatgaat aaaacttgga aacggcaggt tttccgccat accgcgcttt ataccgccat
      241 attgatgttt tcccataccg gcgggggggg gggcaagcgc aagcgcaaac gcataaatac
      301 gctattgtaa tgaacgcgca aaatctgccc gaggtaaagt gggggaatgc atattcatca
      361 ttggcgcaca aaagcaatga acgcgaagtt actcatacga gttattttgt cggaccaaaa
      421 aagaacgtca gtttctcatt caggaatacc gatgaagttg ttgctcaaaa aaacggtact
      481 atcgttttcg gcgcggcgac ctacctgccg ccctacggca aggtttctag ttttgatacc
      541 gctaagctga ccgagcgcaa aaatgccgtt gatcagatta atacgaccca ccctgggttg
      601 ataggctaca actaccaaga tgtcacttgc tccagcggaa actgtcctga acttagctat
      661 agaacccaat ttaccttcaa taacgggttg aaaagaaatg gaaacggcgg caggctggat
      721 atatacgaag acaaaagccg cgacagttcg cccatttaca aattgaagga tcatccttgg
      781 ttgggcgtgt ctttcaattt gggcggcgag agctccttca aaccaaagag acaaggttct
      841 ttggtatctt cttttagcga ggatgtgacg cagcaaaatg gtacacaaga ccaatacaaa
      901 agcaaaaacc tcgtttatac gacagacgat tacaatagta agggtaataa aaaccatcag
      961 gacagacacc acgccgtcgc cttttatctg aacgccaagc tgcatttgct ggataaaaaa
     1021 caaatcaaaa atatcgcgca aggcgtaaca gttgatttgg gtaccttgaa aacgcgcatc
     1081 gagccgacgc aagagtggaa aaaccgggga gggaactatt ttaataatgg tacttggacg
     1141 tatgaagaga aaggatcagt cagcgtcaaa ctcaaattgc cggaagtcaa agcaggccgc
     1201 tgcatcaata aaccgaaccc caatcccaac gctaaagccc tttcccccgc cctgactgcc
     1261 cccgcgctgt ggttcggagc tgggcaagat ggtaaggcgg agatgtattc cgcttcggtt
     1321 tccacctacc ccgacagttc gagcagccgc atcttccttc aaaatctgaa aagaaaaaac
     1381 gaccccaaca aacccggccg ctattccctc gcagacttga gtgcgtcgga tattcaaagt
     1441 aaagagccga ctttcacaag tcggcaaaca atcatccgat tggatggcgg cgtacagcag
     1501 atcaaactgg atcaaaacaa tactgaggtc gtcaatttta atggaaatga cggcaaaaac
     1561 gacactttcg gcattgttca ggaatatggc ttcaagccta atgacgacga gtggaaaaaa
     1621 gtgctgctgc cttggacggt tcgtgctttc aatgatgacg gtcaatttaa cacagtcaac
     1681 aaagaagaaa acaacggcaa gccaaaatac agtcaaaaat accgcagccg cgacaacggc
     1741 aagcacgagc gcaatttggg cgacatcgtc aacagcccca tcgtggcggt cggcgggtat
     1801 ttggcaaccg ccgcgaacga cgggatggtg catatcttca aaaaaaacgg cggcagtgat
     1861 gaacgcagct acaatctgaa gctcagctac atccccggca cgatgccgcg caaggatatt
     1921 gaaagcaaag actccaccct tgccaaagag ctgcgcgcct ttgccgaaaa aggctatgtg
     1981 ggcgaccgct acggcgtgga cggcggcttt gtcttgcgcc aagtcgaacg gaatgggaaa
     2041 gaccatgtgt ttatgttcgg cgcgatgggc tttggcggca gaggcgcata cgccttggat
     2101 ttgaccaaag ccgacggcaa tgacccgaaa aatgcctctt tgtttgatgt gaagaatggc
     2161 gataatagcg cagaattagg ctacaccgtc ggcacgccgc aaatcggcaa aacccacaac
     2221 ggcaaatacg ccgccttcct cgcttccggt tatgcgacta aagacattaa aagcacagaa
     2281 aatcaaaccg cgctgtatgt atatgatttg gaaaacaaca acggtacgcc gattgcaaaa
     2341 atcgaagtga aggacggcaa gggcgggctt tcgtccccca cgctggtgga taaagatttg
     2401 gacggcacgg tcgatatcgc ctatgccggc gatcgcggcg gcagtatgta ccgctttgat
     2461 ttgagcagtc aagatcctca acaatggtct gtacgcacca ttttcagcgg caacaa
//
LOCUS       DAXHNE010000196         2506 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.196, whole genome shotgun sequence.
ACCESSION   DAXHNE010000196 DAXHNE010000000
VERSION     DAXHNE010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.196"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            170..793
                     /gene="lolA"
                     /locus_tag="ACFM4Z_001967"
     CDS             170..793
                     /gene="lolA"
                     /locus_tag="ACFM4Z_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFC6021940.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            1066..2205
                     /locus_tag="ACFM4Z_001968"
     CDS             1066..2205
                     /locus_tag="ACFM4Z_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6021941.1"
                     /translation="MKKTLVAAAILSLALTACGGGNDTAAQAPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFNPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            2339..>2506
                     /locus_tag="ACFM4Z_001969"
     CDS             2339..>2506
                     /locus_tag="ACFM4Z_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6021942.1"
                     /translation="MDLSIVVPIYNVESYLEACLSSIEPILSNENVELILVNDGSKDG
                     SEDICYKYIDKI"
BASE COUNT          680 a          728 c          622 g          476 t
ORIGIN      
        1 gcggcacaca ttaaagggca gcgtggcgcg cctgtctttt ccgagcggtc aaaaaaatca
       61 gccctcggaa aacgcggttt gcaaaatgca aaccgcccgt aacgccgccc atatgattgt
      121 tttgccgcgc cgatacttta cgccacaccc atcccgacaa ggaaaaataa tgatgaaacc
      181 gcacaacctg ttccaattcc tcgccgtttg ctccctgacc gtcgccgtcg cttccgcaca
      241 ggcgggcgcg gtggacgcgc tcaagcaatt caacaacgat gccgacggta tcagcggcag
      301 cttcacccaa accgtccaaa gcaaaaagaa aacccaaacc gcgcacggca cgttcaaaat
      361 cctgcgcccg ggcctcttca aatgggaata cacttcgccc tacaaacaga ctattgtcgg
      421 cgacggtcaa accgtctggc tttatgatgt cgatttggca caagtgacca agtcgtccca
      481 agaccaggcc ataggcggca gccccgccgc catcctgtcg aacaaaaccg ccctcgaaag
      541 cagctacacg ctgaaagagg acggttcgtc caacggcatc gattatgtgc tggcaacgcc
      601 caaacgcaac aacgccggct accaatacat ccgcatcggc ttcaaaggcg gcaacctcgc
      661 cgccatgcag cttaaagaca gcttcggcaa ccaaacctcc atcagtttcg gcggtttgaa
      721 taccaatccc caactctcgc gcggcgcgtt caagtttacc ccgccaaaag gcgtggacgt
      781 gttgagcaac tgatgccgtc cgccccgatg ccgtctgaat gccgccgagg cttcagacgg
      841 cattttcacg caggcggaac aatgtcccgc attaccgccc gatcgggcat cggaacggca
      901 aaccggtgaa aattaacggt tgcgccccgg ctgtttttgc cgtttaatgc aaaccttgct
      961 gcaccaaggg ccaagaaagc cgaccggccg cccccacagc ttccgatgca ggcggccagg
     1021 ccgtccctgc aatgtttttt atttttgaac gaaaggtcga aaaccatgaa aaaaacactg
     1081 gtggcggcgg caatcctgag cctcgccttg actgcgtgcg gcggcggaaa cgataccgcc
     1141 gcccaagccc cctccgccaa gcccgaagct gaacaatcgg gcaaactcaa catctacaac
     1201 tggtcggatt atgtcgatcc cgaaaccgtc gccgcctttg aaaaagaaac cggcatcaag
     1261 acgcgttccg attattacga cagcaacgaa acactggagg caaaagtcct gaccggcaaa
     1321 tccggctacg acctgactgc gccgtccatc gccaacgtcg gccggcaaat caaagcgggc
     1381 gcgtatcaga aaatcgacaa ggcgcaaatt ccccattacg gcaacatcga taaagatttg
     1441 ttgaaaatga tggaagccgt cgatccgggc aacgaatacg ccgtccccta tttctggggc
     1501 atcaacacct tggcaatcaa cacgcggcag gtgcaaaagg cattgggtac ggacaagctg
     1561 cccgaaaacg aatgggattt ggtgttcaac cctgaataca ccgccaaact caaatcctgc
     1621 ggcatcagct atttcgacag cgcaattgaa cagattcctt tggcgttgca ctatttgggc
     1681 aaagacccca acagtgagaa tcccgaagac atcaaagccg ccgtcgatat gatgaaagcc
     1741 gtccgaggcg acgtgaaacg cttcagctct tccggctata tcgacgatat ggcggcgggc
     1801 aacctgtgtg ccgccatcgg ttacggcggc gatttgaaca ttgccaaaac ccgtgccgaa
     1861 gaagccgcaa acggcgtgga aatcaaagta ttgaccccga aaaccggcgt gggcgtgtgg
     1921 gtggattcct ttatgattcc gcgcgacgcg caaaacgtcg ccaacgccca ccgctatatc
     1981 gactacacgc tccggcccga ggtggcggcg aaaaacggca gcttcgttac ctacgcgccc
     2041 gccagccgtc ccgcgcgcga gctgatggat gaaaaataca cctccgacgc atcgattttc
     2101 ccgaacaaag aactgatgga aaaaagtttc atcgtatcgc ccaaatccgc agaatccgcc
     2161 aaactgggcg tgaagctgtg gcaagggctc aaggccggaa aataaccgca acccctgccg
     2221 tctgaaacct ttcgggcggc aggaaacggc gcgtccgtta tcaaacaggg ggggcgtttc
     2281 ccctcctgcc ggttatgatt gggttaagat taaaatgatt tagtaaaatg agaaagatat
     2341 ggatttaagt atcgtagttc ccatttataa tgtcgaaagt tatttggaag cgtgtttaag
     2401 ttccatagaa cctatattaa gtaatgaaaa tgtcgaactt atccttgtga atgacgggtc
     2461 aaaagacgga agtgaagata tatgttataa atatatagat aaaata
//
LOCUS       DAXHNE010000197         2500 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.197, whole genome shotgun sequence.
ACCESSION   DAXHNE010000197 DAXHNE010000000
VERSION     DAXHNE010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.197"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            398..1606
                     /locus_tag="ACFM4Z_001970"
     CDS             398..1606
                     /locus_tag="ACFM4Z_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFC6021943.1"
                     /translation="MSEAEYFSHFISNGNGKLLEIPQRRGRQDGVFIDWLSFTLHEDS
                     LLKVSGCPLVSDAEYMFVLSKKLEEILGFGITSRCKSKGNKFYDSMFRLGSEEVDYGE
                     VHYGGQRNTVLIELKGVGCNIANPGWELRLKQFLEDSLRPRITRVDLALDFFDGEYTP
                     EQALLDHDNGFFDNSNMRPKSEMVGTAWRREDGSGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNHGDMEIPLDILINQGSYFSGAFPICQKFKNMPNPERFDYRKKV
                     ANLTFQHKLRYAKNAVGKLINFMFDMGFDSDEIVRYLKADLGYPKGLEPEKYSLAGLK
                     ESLKFGFIHEQPDVDLEVELEELGIIKFKQSDKFDPDKRLFDPHHDVESERQYQLYLD
                     RMYDLHANQN"
     gene            1627..1938
                     /locus_tag="ACFM4Z_001971"
     CDS             1627..1938
                     /locus_tag="ACFM4Z_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021944.1"
                     /translation="MFNQTQTVTYPATFLGAKKFKGEIDGSNIDTCSVLVATPLPAQS
                     GNAVGFTAAQMKFGDSKNFSRLENLKYPCEVMVTVEMTSTGKGMVPSLIDFQVAEKPK
                     G"
     gene            1942..2142
                     /locus_tag="ACFM4Z_001972"
     CDS             1942..2142
                     /locus_tag="ACFM4Z_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021945.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEIGGGFQIFQFFVKSE"
BASE COUNT          783 a          420 c          580 g          717 t
ORIGIN      
        1 cctttgacag ccaagtgaaa ggggcttttt tatgtcagta gcaaatgtaa tattttcttg
       61 ttcctattgg agaatattta aaaaatcaga ttattgcgtt ttatgtttct atcagttcag
      121 gcatggtgaa ccgcataaac tcgctgaaca agaaaatttt tcaaagcttt atcaggcgtt
      181 cgattatata gattcggttg gctcgaattt tccggtaatt atcacaacag acggttgtgg
      241 tctttcttct tgatctttaa cagtttgtca ggattgggct ttcggtcgtt gaccgttgga
      301 cgcgctttag cgcggcagac gggaaacggc tgaaagcccc cccctgacta acaggggggg
      361 agcgaaataa aacaaatccc taaaggtact gaacaaaatg agtgaagcag aatatttttc
      421 ccatttcata tctaacggca atgggaagtt attagaaatt ccgcaacgta ggggcaggca
      481 ggacggcgtt tttatagatt ggttgtcatt cacactgcac gaagattcct tgctgaaagt
      541 ttccggatgc cccttagttt ccgatgccga atatatgttt gttttaagca aaaaattgga
      601 ggaaatatta gggtttggca tcacgagcag atgcaaatcg aagggcaata aattttacga
      661 ttcgatgttt aggttgggat cggaagaagt tgactacggc gaagtccatt acggaggtca
      721 gcgaaatacg gttttaatcg aattgaaagg tgtaggttgc aacattgcaa atccaggttg
      781 ggaattgagg cttaagcagt ttttggaaga ttcattgagg ccgaggataa cgcgggtaga
      841 tttggcactt gatttttttg atggggagta cacgccggaa caggcacttt tggatcacga
      901 taacggtttt ttcgataaca gtaacatgag gccgaaatct gaaatggttg gaacggcttg
      961 gcggagagag gacgggagcg gcaagacatt ttatgtaggt cgcaagaaaa attctcgttt
     1021 tgtgcgtgtt tatgagaaag gcaggcagct aggcgataaa gaaagcaagt gggtaaggtt
     1081 cgaaattcag tttaatcatg gagatatgga aatacctttg gatattctga taaatcaagg
     1141 ttcttacttt tcaggcgctt tcccgatttg tcagaaattt aaaaatatgc cgaatccgga
     1201 aaggttcgat taccgtaaaa aagtggctaa tttaactttt cagcataaat tgagatacgc
     1261 aaaaaacgcg gtcggcaaac tgattaattt catgtttgat atgggttttg atagtgatga
     1321 aattgtcaga tatctgaagg cagatttggg gtatcccaaa gggctagaac ctgaaaaata
     1381 ttcgttggcc ggattgaagg aatctttgaa attcggcttt atccacgaac aaccggatgt
     1441 agatttagag gttgaattgg aagaactcgg aattatcaaa tttaagcaat cagataaatt
     1501 cgatccggat aaaaggcttt tcgatccaca tcacgatgta gaaagtgaga ggcaatatca
     1561 gctttatctc gacagaatgt atgatcttca tgcaaatcaa aattaaccta aaaaggaaaa
     1621 attaatatgt ttaatcaaac tcaaactgta acttatcccg caactttttt aggagctaaa
     1681 aaattcaaag gcgaaattga tggctctaat atcgacactt gttccgtatt ggttgcaaca
     1741 cctttgccgg cacagtcggg aaatgctgtt ggattcacgg cagcacaaat gaagttcggg
     1801 gacagtaaga atttctcaag attagagaat ctcaaatacc cgtgcgaagt tatggtaacg
     1861 gttgaaatga cttcgacagg taagggcatg gttccttcat taattgattt tcaggtggca
     1921 gaaaagccga aaggttgatt tatgaaattt gaagaacgtt tcatagttca agacttggaa
     1981 acgcatgact ttatttatcc cgatcctttc ggtgatgtgg ggtttactca aaatattaaa
     2041 tcagcaggtc aatttgaaag ctacgaagat gcgttgaatt caggcataaa tgaaataggc
     2101 ggaggattcc agatatttca gttcttcgta aaatcggaat aaaagaaaaa caggctcggc
     2161 gggcggtctg tcaacctttc acaaagcccg caacaaagga ttaaaaatga ataaagcaac
     2221 tttaattatc ggattttttg tttgcctcat tcttttcatt ttctcaattc tctatttcat
     2281 aaattaaaaa caaaattcgg gagcaggctt cgcccggatt taaacctaag aagccgtatt
     2341 actttaaata tcaaaaaagg aaaaaaacga tgaacatcgt taaaaaatac gctgtaaaag
     2401 cagccttggc agccggtatc ttcacaccgg ccattgttat ggcagatacc tttgatgcat
     2461 ccgcgattgg tacgcaagta gcgaatgtaa tcatgggttt
//
LOCUS       DAXHNE010000198         2480 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.198, whole genome shotgun sequence.
ACCESSION   DAXHNE010000198 DAXHNE010000000
VERSION     DAXHNE010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.198"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(13..819)
                     /locus_tag="ACFM4Z_001973"
     CDS             complement(13..819)
                     /locus_tag="ACFM4Z_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFC6021946.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKRELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDFPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRSF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1039..2409
                     /gene="ffh"
                     /locus_tag="ACFM4Z_001974"
     CDS             1039..2409
                     /gene="ffh"
                     /locus_tag="ACFM4Z_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFC6021947.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTVGKLARLLKNNQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGIVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMRGIK
                     GMFPGL"
BASE COUNT          685 a          627 c          642 g          526 t
ORIGIN      
        1 tgttggctct tcttatcttt tcagaatgat ttccaatacg aacttgctgc ccatataggc
       61 aatcataagg ctgacaaatc cgatgatggt ccacacggcg gcttttttgc cgcgccatgc
      121 ggtcatgctg tgcttgagca gcagtccgcc gtaaatcagc catgacaata tgccgaatac
      181 ggttttatgg gtaaaggtca tgggtttgcc gaatacggct tcggcaaaaa atgttccgct
      241 gacgacggaa taggtcagca ggatgaaacc tgcccacatg gcctggaaca tgagtttttc
      301 caaactgagc agcgacggca ggaatcctgc gagcttggag aagctcctgc ggtgcagact
      361 ccgattcagc agcagggtca aaacggacaa taatgttgcg atgccgaaca gcccgtatgc
      421 gagcagcgaa gttccgatat gcagcataaa gggaaagtcg gtaatttcat atcccgagaa
      481 ttttccagga aaaaccaaac ctgacagcag catcagtgcg gcgcaaggat acagcagcaa
      541 ctgcactccg cgcagcggat aaaagaagct gccggcaaaa taaataaaca gcatcatcca
      601 aacaatcagg ctgccggaat acccgaagcc cataatgatg attttgtctt gaatgaccgg
      661 catcagcagt gccgcgccgt ggacggtcaa tgccgcgccc aaaaccggca attccctctt
      721 ccacgggtaa tcccgaccgc gcccctgctg ttggcagtgc catgcaaatg cacccaatcc
      781 tgcgtaaacc gccgtcaaaa agatgaaaac tgtcggcatg gtggactttc tctatctata
      841 ctgttgcgcc gtctgcggcc gcttatgaaa tattggaact tttaacgttg gaattgtaaa
      901 atccccgttt cgggcaagcc ttgacggatt tgccgatatg ctgtccggca cacaagccgc
      961 atcaaattat tttgatttta ttttaacaaa gaatgcccct gatggggcaa gctattctta
     1021 ttcagaccaa ggaccagtat gttagacaat ttaaccggcc gcttcagcaa tgtcttcaaa
     1081 aacatccggg ggcaggccaa actgaccgaa gacaatatta aagaggcctt gcgcgaagtc
     1141 cgcctcgccc tgcttgaggc ggatgtcgcc ctgcctgtcg tcaaagagtt cgtcaacaac
     1201 gtcaaagaaa aggccctcgg tcaggaagtg gcgggcagcc tgacgccgga tcaggcattt
     1261 atcggcgtgg tcaacaaagc cctgaccgaa ctgatgggca gggaaaacaa aacgctggat
     1321 ttgtcggttg cgccgcccgc cgtcgtttta atggcaggtt tgcagggcgc gggcaagacg
     1381 acgaccgtcg gcaaactcgc ccgcctgctg aaaaacaatc agaagaaaaa ggttttggtc
     1441 gtatctgccg acgtttaccg ccctgccgcg attgaacaac tgcgcctgtt ggccgaacag
     1501 gtcggcgtgg actttttccc atccgatacc aaccaaaaac cggttgaaat tgcaactgcc
     1561 gccgtcgatt acgccaaaaa acatttttac gatgttttga tggtcgatac cgccggtcgt
     1621 ctggcaatcg atgaagagat gatgaacgaa atcaaagccc ttcacgcggc ggttaacccg
     1681 gtggaaactt tgttcgtcat cgatgcgatg ctgggtcagg atgcggtgaa cactgctcag
     1741 gcatttaatg aagccctgcc gctgaccggc atcgtgttga ccaagatgga cggcgattcg
     1801 cgcggcggtg cggcattgtc cgtacgccat gtaaccggca aaccgattaa atttatcggc
     1861 gtcggcgaaa aaatcaacgg cctcgaacct ttccaccccg accgtcttgc cagccgtatc
     1921 ctcggcatgg gcgacgtatt gaccctgatt gaagacgtcc aaaaaggtat tgatgaagaa
     1981 gccgccgcca aaatggcgaa aaagctacaa aaaggcaaag gcttcgacct caacgacttt
     2041 aaagaacaaa tccagcaaat gcgcaatatg ggcggtttgg aaaacctgat gtcgaaaatg
     2101 ccgggcgaac tgggtcaaat atcgaaacaa atccccgaag gaacggctga aaaagcgatg
     2161 ggcaaagtgg aagccatcat caactcgatg actccaaaag aacgcgccaa ccctgccctg
     2221 ctcaaagcca gccgcaaacg ccgtattgca atgggtgcgg gcacaaccgt gcaggaagtg
     2281 aacaaattgc tcaaacagtt tgaacaaatg caacagatga tgaagatgtt cagcggcaac
     2341 ggcttgggca aactgatgcg tatggcgaaa ggaatgaggg ggataaaagg gatgttcccg
     2401 ggtttgtaag ccgatttaac agaaaacgcc gtctgaaaag tttcagacgg cgtttttgtt
     2461 ttatattctg atttatagtg
//
LOCUS       DAXHNE010000199         2443 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.199, whole genome shotgun sequence.
ACCESSION   DAXHNE010000199 DAXHNE010000000
VERSION     DAXHNE010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.199"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(120..1571)
                     /locus_tag="ACFM4Z_001975"
     CDS             complement(120..1571)
                     /locus_tag="ACFM4Z_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC6021948.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     IGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            complement(1594..2214)
                     /locus_tag="ACFM4Z_001976"
     CDS             complement(1594..2214)
                     /locus_tag="ACFM4Z_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC6021949.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
BASE COUNT          763 a          578 c          570 g          532 t
ORIGIN      
        1 aggaataaga attcgggaca aaatgtttaa accattttgt cccgactgct gtgatgcggt
       61 ttttttgaac tagcaggacg ttacctaaaa cctgcgttgc ctgaaaccct ttaagacggt
      121 taaatccctc tgccgtattt gtattctaca atcatattat cgtcggtaat gacttccgcc
      181 ccagccgaag gttccgtcat ctgaatcagc atacgagaga caaccttttg tgctgcagca
      241 tccacggtgc tgctgtcaaa tacgtgcctg ccgctttccg gccaaatcaa ccgggagaga
      301 cgttgcttga gcagttcttt attagggaaa actaccgggg ttgccgagcc gactaccata
      361 tgcccatagc ggtatgcata gggaatactg tgtacggcgg tagcaaaagc atgcgggctg
      421 tgcgtggtat taaacattac aataccatcc ggggtaaggt ggctttgcac ctgttttaaa
      481 aattccgcac tcaacaggtt ggtggaatag gcacgccagt accaagtcgt attcatcaaa
      541 atcaggtcga atttttcatc aggatggcga cgcagccatt tcctaccgtc atccaataca
      601 atttcaacac gtttgtcctg caaaagcggg gcgatttgcg gctcgtccgc gataaggcta
      661 cggtatgccg gattgatttc cgcaacaatc atcgactgca tttccggaat ggcagacaag
      721 acgcgcgccc acgaacctgt actcaatcca acgacgaaaa tgcggcgtat gccagacttc
      781 agggagggta gcagataggc acgttcgatg ccgttgacac tattgaatac atcggtattg
      841 tatgcgccgt cgtatacatt cgccccataa acaaccttat caccatctct atggtaaacc
      901 gcaacaatgc cgtgtttgtt ttcaatcagc ctatccggac ggtcagcaat attttgaaag
      961 acagaatccg gcagtaggaa catgaggatg ccgaacatta gggaaactgc taccgacact
     1021 gcattcagtc ggagactttt ttggaacagt gtacaaaaca aagggacagc agcagaaatc
     1081 aaacagatga gcaggtaaat ctgttgggtg gacaagaaat caagtatcac aaagccgata
     1141 aggaccggac ccaatgcact gccggcaacg ttggcgaaat aaacattgga aacctgtcgt
     1201 ccggatttgt tgccatccgt acccacatgg tgtacgagcg ggaaaatcaa ccctctgacg
     1261 acggcagaca gggtaatgaa gataccggcg tggtggacga agccggaaaa acccgtcaac
     1321 aaccacgcag cacccaaaat caaaaagtcg gcaatacccg cccacaagaa gcactgcccg
     1381 ataaagggaa tatcaacaaa gcggctgcgg caaatccgtt tgccaaaata cgcgccgatg
     1441 gcgataccgg tcagaaaaca ggcaagagta aatgaaaatg cctgaggcac ggactgtgct
     1501 gcgaacgaaa acatccttac ccacaagact tctataccca agctcaataa gccgctaagg
     1561 aaagaaagca tataaattaa actagtattc ggtttagtgt tcactatatc catcctttct
     1621 gtaacgcagc catactgaag cagcaatcag aaggttaaag caggctgtca gcgcaatggt
     1681 ttgggagagg gtaaaaaaga cgtagaaaaa ttcggcggcg gcaagcgatc cgagtgccgc
     1741 acccaaagtg ttgaaaaaat ataaggtacc gatagactcg ccaacattat gtattttccg
     1801 gttaaaaaaa caggtcagca agggcaaggt cgcgcccatc ataaaggtag gaagcagcaa
     1861 taagaggaaa ttggcagcag cgatgatggg caaatcagcc tcaactaaaa gatgccccaa
     1921 gccggaaatc agacccctgc ttaccaaacc gaacagaccg atggatactt cagcgatgca
     1981 aaacaggggg atgatacttg aaggaaaacg gtcagcaatg cgtccaccga aatacgcacc
     2041 tacacccaag ccgaccataa atacagaaat aatgacagta atcgaactca aatcgatacc
     2101 tatgtgactg aatagaagcc tctgccagct gacctggtaa atcagggcgc agaatccaga
     2161 ggcgaaaaac accagcgaca atcctttcag ggttttactt gcggtcgaat tcatattttc
     2221 tattttcaaa taggcggaac tataaatgaa atgtatcaat gtgtaaacat ttgtattaac
     2281 cgtaggaaat caccgtattg ctcaaactgc aggccgggaa aataaactcg taagcccgca
     2341 aacggcgtat cactgttcaa actgctgtgc ggcttcacgg tgttataaaa ataatggggc
     2401 tgtcctggac aactaagata aactcgattt tactaattgt ttt
//
LOCUS       DAXHNE010000200         2433 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.200, whole genome shotgun sequence.
ACCESSION   DAXHNE010000200 DAXHNE010000000
VERSION     DAXHNE010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.200"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            35..1390
                     /locus_tag="ACFM4Z_001977"
     CDS             35..1390
                     /locus_tag="ACFM4Z_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HFC6021950.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHSLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKSY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWIELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2404
                     /locus_tag="ACFM4Z_001978"
     CDS             1394..2404
                     /locus_tag="ACFM4Z_001978"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFC6021951.1"
                     /translation="MSDTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQEFS
                     VLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGR
                     NALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDRLTV
                     TYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDLEVF
                     DFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDRNGL
                     VIFTAAPQDFARHDAGVYDALATEAQTLPDSLHWPEFGNIRLNKGDTREATIAYGIES
                     LS"
BASE COUNT          436 a          668 c          740 g          589 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcagatg agccgcatct tccagacgct
      181 cggtgccgat ccgcacagcc tcggctggtt ctttatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggcc attactccga ccgcacttgg aagccgcgtt tgggcggccg
      301 ccgtctgccg tatctgcttt atggcacgct gattgcggtt attgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctatgc gtcgctggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgttggacg tgtcgtcgaa tatggcgatg cagccgttta agatgatggt
      481 cggcgatatg gtcaacgagg agcagaaaag ctacgcctac gggattcaaa gtttcttagc
      541 gaatacgggc gcggtcgtgg cggcgattct gccgtttgtg ttcgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccgca gaccgtggtc gtggcgtttt atgtgggtgc
      661 ggcgttgctg gtgattacca gcgcgttcac gattttcaaa gtgaaggaat acgatccgga
      721 aacctacgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggat
      781 cgaactcttg aaaaccgcgc ctaaggcgtt ttggacggtt actttggtgc aattcttctg
      841 ctggttcgcc ttccaatata tgtggactta ctcggcaggc gcgattgcgg aaaacgtctg
      901 gcacaccact gatgcgtctt ccgtaggtta tcaggaggcg ggcaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaataccat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtgacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggcc tgtttaacgg ctctatctgt atgcctcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tccctatgct gggcggcttg caggccacta tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttccgtgttc ctgattaaag aaacacacgg
     1381 cggggtttga gcgatgagcg atacccccgc tacccgcgat ttcggcctga tcgacgggcg
     1441 tgccgtaacc ggctatgtgc tgtccaaccg gcgtggtacg cgtgtctgcg tgctggactt
     1501 gggcgggatt gtgcaggaat tttccgtttt ggcagacggc gtgcgcgaaa acctcgtggt
     1561 gtcgttcgac gatgcggctt cctatgcgga caatccgttt cagattaaca agcagatagg
     1621 gcgcgtggcc ggacgcatcc gcggtgcggc gttcgacatc aacggcagga cttaccgcgt
     1681 ggaggccaac gaaggcagga acgcgctgca cggcggttcg cacgggctgg ccgttacccg
     1741 tttcaacgcg gtggcggcag acggccgttc ggtggtgctg cgcagccgcc tgcaacagtc
     1801 ggccgacggt tatcccaacg atttggattt ggatatttcc taccgcttgg acgaggacga
     1861 ccggcttacc gttacctatc gcgccaccgc gctcggcgac acggtgttcg acccgacgct
     1921 gcacatttac tggcggctgg acgcgggcct gcacgatgcg gttctgcata ttccgcaggg
     1981 cggacatatt ccggccgatg ccgaaaaact gcccgtctca acggtttcag acgacctcga
     2041 agtatttgat ttcagccggc ccaagccgct ggatgccgcc gttgccgccc tgcgccgcga
     2101 aacgggtcgg gccggttttg acgacgctta ccgcgtgccg tccgatatag gccgtcccgc
     2161 cgctgtgttg caagccggac gccgccgtcg tatcagcata tacagcgacc gcaacggctt
     2221 ggtcatcttt accgccgccc cgcaggattt cgcgcggcac gatgcgggcg tttacgacgc
     2281 gctggcgacc gaggcgcaga cgctgcccga cagcctgcat tggcccgagt tcggcaatat
     2341 ccgtctgaac aagggtgata ccagggaggc gacgattgct tacggcatcg aatccctttc
     2401 ttaggagctt cctaacaccg gttgcagacg acc
//
LOCUS       DAXHNE010000201         2429 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.201, whole genome shotgun sequence.
ACCESSION   DAXHNE010000201 DAXHNE010000000
VERSION     DAXHNE010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.201"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(1..524)
                     /locus_tag="ACFM4Z_001979"
                     /pseudo
     CDS             complement(1..524)
                     /locus_tag="ACFM4Z_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            784..2256
                     /gene="pyk"
                     /locus_tag="ACFM4Z_001980"
     CDS             784..2256
                     /gene="pyk"
                     /locus_tag="ACFM4Z_001980"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFC6021952.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVTAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQVEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
BASE COUNT          646 a          656 c          607 g          520 t
ORIGIN      
        1 ggttccaaaa gttctcaatt ccattaatat ggttttgtcg ttcggcaaaa tgtgtgctgt
       61 gattgatacg aaaacgaagt ttcagcgaag ctaaaatggc taaattcgcg cacatctaat
      121 acatcatagc tacgataaca atccgtataa aaaatgctgt caggtttcac ttgttcacgg
      181 ataataggaa ataaagtagc ggtttgagta ttcggtactg taaccgtata aaccttacca
      241 tttcgcttca aaagaccgaa tacggcgact ttaccggcag caccgcgacc gcgtttgcct
      301 ttgcgttgtc cgccaaaata actttcatct gcttctactt cgccatcaaa catttccaaa
      361 tgcggactgt tttgataaat aagtaatcgt aaacgatgaa aataataggc tgcggtattt
      421 ttattaacgc ctactaactc tgctgccgtt cttgcagtta cacctgtgac aaacagttca
      481 atgagtttat tttgtttata ctggcttaga cgactttttc tcatagggat aattctaact
      541 taatttgaat ttccctagtt atctaggaca gccccaaaat aaatttagcc taagtcataa
      601 aaccgtgtaa ttttgcgcca aaaaaggcgg tttgcaggta aaatatcaaa ctattaattt
      661 aaatcatatt tttacagaat attccgccgc gttcatcaaa tggacatcaa accggttcca
      721 aatttctgta attttgtaac aaaataccgc aaaacacccg attgagacca aaaggacttt
      781 catatgaacc agacaagccg cgatctgacc cgcatcagcc acaacactaa aatcgtcgcc
      841 acccttgggc cgggcagcaa caacgtcgaa ctgttggaag acatgatccg cgtcggcggt
      901 ctgaacgtcg tccgcttcaa cttcagccac ggcacgcccg aattccatca ggaaaacgcc
      961 cgcattgtgc gcgaggcggc aaaacgcgcc ggacaggaaa tcgccatcat tgccgacctg
     1021 caagggccga aaatccgcgt gggcaaaatc gccggcggcg gcatcgaatt gaacaaaggc
     1081 gaaacgctgg tactggatgc cgcgctcgaa ggcgaaggca cgcgcgaggc ggtcggtttg
     1141 gactaccgcg acctgcccga cgacgttacc gcaggcgacg tattgtggct ggacgacggc
     1201 ctgctgaccc tgaccgtgga atccgtcgaa ggcagcagga ttatcacaag ggtggaaaac
     1261 agccacgtcc tgaaaagcaa caagggcatc aacaaacgcg gcggcggtct atctgcaggc
     1321 gcgttgaccg aaaaagactt ccgcgacctg aaaaccgcga ttgccatcgg ttgcgactac
     1381 ctcgccatca gctttgtgaa atccgccgaa gatttgcata tcgcgcgcgc caaagtcgaa
     1441 gaagaaatga agggcagcac tgccgtgcgc cccggtttgg tttccaaaat cgaacgcgta
     1501 gaagcgattg aaaacttgga cgaaatcatc ctcgccggcg acggcattat ggttgcgcgc
     1561 ggcgacttgg cggtcgaagt cggacacgcc gccgtccccg ccctgcaaaa acggatgatc
     1621 cgccgcgccc gcgagttgcg ccgcttcagc attacggcga cgcaaatgat ggaatcgatg
     1681 attaccaacc ccgtaccgac ccgcgcggaa gtcagcgatg tggcaaacgc ggtattggac
     1741 ggtaccgatg cggtgatgtg ttccgccgaa accgccgtcg gcgcgtatcc gtttgaaacc
     1801 gtcagccaaa tggcgattat ctgcgcggct gcggaaaaag agcaggattc gctcaacggc
     1861 gttgccgaac aggtcgagta tcccgaagcg gtcagcacca acctggcggt tgccggcggt
     1921 gcggtcagcg tggcgcgcgc ggttcacgcc aaagccatcg tcgccctgac cgaaagcggt
     1981 tcgaccgcct ttgaaatcag ccgccacaac atcaccctgc cgattttcgc gctgaccccg
     2041 agcgtttccg cccaacgccg tatggcgatg taccggggcg tgcgcccgct gattttggca
     2101 accagtaccg accacgacac ggcgttaaac gaagtcgaaa cgatgctggt ggaacacaac
     2161 atcctgcatt ccggcgacca atacatcatt accagcggtt cgcaaatgcg cgaatccggt
     2221 tcgaccaaca cactggaagt gctgcgcgtc aaataatccg ccctgagtgg aaaatgccgt
     2281 ctgaagccga tgcccgaggc ttcagacggc attttttgcg gcggcgcggc ggttcgggca
     2341 aactctgaaa aaattgtgca aatccggcaa catcggataa aatcgagtac ctatactaaa
     2401 gcgaaacaag gcatttccga ctgcctttt
//
LOCUS       DAXHNE010000202         2388 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.202, whole genome shotgun sequence.
ACCESSION   DAXHNE010000202 DAXHNE010000000
VERSION     DAXHNE010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.202"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            44..2257
                     /locus_tag="ACFM4Z_001981"
     CDS             44..2257
                     /locus_tag="ACFM4Z_001981"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC6021953.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPNNDKKLVLAARSLA
                     EAHYPADAATPYGEPLPDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVTE
                     RCNSTVCELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILIDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPIPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEQAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVTDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          625 a          779 c          566 g          418 t
ORIGIN      
        1 ctgttacaat aaactcgttt ttatcggaac ggaagacccc atcatgaccg ccatcagccc
       61 gattcaagac acgcaaagcg cgactctgca agaattgcgc gaatggttcg acagctactg
      121 cgccgcgctg ccgaacaacg ataaaaaact tgtcttagcc gcccgttcgc tggcggaagc
      181 acattacccc gccgatgccg ccacgccgta tggcgagccg ctgcccgacc acttcctcgg
      241 cgcggcgcaa atggttcatg aactcgacct gctccccgat gccgtcgccg ccaccctgct
      301 tgccgacatc ggacgctacg tccccgattg gaacctattg gttaccgaac gctgcaacag
      361 caccgtttgc gagctggtca aaggtgtgga cgaagtgcag aaactcaccc acttcgcccg
      421 ggtggacagc ctcgccacac cggaagaacg cgcccagcag gcagaaacta tgcggaaaat
      481 gctgctggcg atggttaccg acatccgcgt cgtgttaatc aaactggcga tgcgtacgcg
      541 caccctgcaa tttttaagca acgcccccga cagccccgaa aaacgcgccg tcgccaaaga
      601 aaccctcgac atcttcgccc cgctcgccaa ccgtttgggc gtgtggcagc tcaaatggca
      661 gctcgaagat ttgggcttcc gccatcaaga acccgaaaaa taccgcgaaa ttgccctgct
      721 tttggacgaa aaacgcaccg aacgcctcga atacatcgaa aacttcctca atatcctgcg
      781 tacggaactc aaaaaataca atatccactt tgaagtcgcc ggccgtccga aacacatcta
      841 ctccatttac aaaaaaatgg tgaagaaaaa actcagcttc gacggcctct ttgacatccg
      901 cgccgtgcgg attctgattg ataccgtccc cgagtgttac accacgctgg gcatcgtcca
      961 cagcctctgg cagcccattc ccggcgagtt cgacgactac atcgccaacc ccaaaggcaa
     1021 cggctataaa agtttgcaca ccgtcatcgt cggcccggaa gacaaaggcg tggaagtgca
     1081 aatccgcacc ttcgatatgc accaattcaa cgaattcggt gtcgccgccc actggcgtta
     1141 caaagagggc ggcaaaggcg attccgccta cgaacagaaa atcgcctggt tgcgccaact
     1201 cttggactgg cgcgaaaaca tggcggaaag tggcaaggaa gacctcgccg ccgccttcaa
     1261 aaccgagctt ttcaacgaca cgatttatgt cctgaccccg cacggcaaag tcctctccct
     1321 gcccacgggc gcgaccccca tcgacttcgc ctacgccctg cacagcagca tcggcgaccg
     1381 ttgccgcggt gcgaaagtcg aagggcagat tgtgccgctg tccaccccgc tcgaaaacgg
     1441 acagcgcgtc gaaatcatta ccgccaaaga agggcatcct tccgtcaact ggctctacga
     1501 aggctgggtc aaatccaaca aggcaatcgg caaaatccgc gcctacatcc gccagcaaaa
     1561 cgccgacacc gtgcgcgaag aaggccgcgt ccaactcgac aaacagcttg ccaaactcac
     1621 gcccaaaccc aacctgcaag agcttgccga aaatctcggc tacaaaaagc cagaagacct
     1681 ctacaccgcc gtcggacaag gcgaaatttc caaccgcgcc atccaaaaag cctgcggcac
     1741 gctgaacgaa ccgccgccca tacccgtcag cgaaaccacc atcgtcaaac agtccaaaat
     1801 caaaaaaggc ggcaaaaacg gcgtgctcat cgacggcgaa gacggtctga tgaccacgct
     1861 tgccaaatgc tgcaaacccg cgccgcccga cgacattgtc ggcttcgtta cccgcgatcg
     1921 cggcatttcg gtacaccgca aaacctgccc ctctttccga cacctcgccg aacaagcacc
     1981 tgaaaaagtg ctggacgcaa gctgggcggc gttgcaggaa ggacaagtgt tcgccgtcga
     2041 tatcgaaatc cgcgcccaag accgctccgg gcttttgcgc gacgtatccg acgcactcgc
     2101 ccgccacaaa ctcaacgtta ccgccgtgca aacccagtcc cgcgacttgg aagccagcat
     2161 gaggttcacg ctcgaagtca aacaagttac cgacctcccg cgcgtcctcg ccagcctcgg
     2221 cgacgtcaaa ggcgtattga gcgttacccg gctttaaata caaaaatgcc gtctgaaagc
     2281 cgaataacgc ttcagacggc attttgattg ccggggtttg ctattttttg ttgcatagtc
     2341 aattaaaaac aaaatagtac aatactcaac tttgaaggtc taaccatg
//
LOCUS       DAXHNE010000203         2352 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.203, whole genome shotgun sequence.
ACCESSION   DAXHNE010000203 DAXHNE010000000
VERSION     DAXHNE010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.203"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..969)
                     /locus_tag="ACFM4Z_001982"
     CDS             complement(<1..969)
                     /locus_tag="ACFM4Z_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFC6021954.1"
                     /translation="MAENGKYLDWAREVLHAEAEGLREIAAELDKNFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLSAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQCRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAARI"
     gene            1066..2121
                     /gene="tal"
                     /locus_tag="ACFM4Z_001983"
     CDS             1066..2121
                     /gene="tal"
                     /locus_tag="ACFM4Z_001983"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFC6021955.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEIAALKQQNLSPKQRYETMAVADVRAACDVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIQVVASFFISRVDGALDTTLPDHLK
                     GKIAIALAKAAYQDWAQYFGSPEFAALETKGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLATRLQED
                     GLKQFEEAFEKLLAPLA"
BASE COUNT          555 a          715 c          614 g          468 t
ORIGIN      
        1 aatccgcgcc gccaaaaggt cgtgcatatt cagcgcgcca atcagcacgc cgtctgcatc
       61 ggtaaccaga agcccgttca catggtttgc ctgcatgact ttcagggctt cggtggcgag
      121 acgttcggcg gagatggttt taggatgcgt atgcatgact tcgtctatcg aaagaccggt
      181 aaaattgtcg cattcttgaa acaggcggcg caaatcgccg tcggtgaata cgcctttcag
      241 acggcattgc ccgtccgtta ccgccaacat gcccagccct ttttcactca tgctgacgat
      301 ggcttctttc aagggcgtgc cgagtcggac ggcagacagg ccgccgcctt tgtgcataat
      361 gtcggcaacg cgcaaaagta ggcgtttgcc gaggctgccg gcaggatggc tcaaggcgaa
      421 atcgtcgggc gtgaacgcgc gtgcgcgcag caggacgacc gccaacgcat cgcccaaagc
      481 catgacggcg gtggtgctgg tggtcggggc aagccccagc gggcaggctt ctttggaaac
      541 cgacgccgtg atgtggatgt cggcatggcg cgccatggtt gaatcggggc gggcggtgat
      601 gcagacaagc gtgatgtctt tgcgtttgag tgcggggatg atggcggcga tttcgtcgct
      661 ttcgccggaa ttggaaatcg cgacgaccac gtcgttgtcc acaatcatac ccaaatcgcc
      721 gtgtgccgct tccgcagggt ggacgaaaaa cgcaggcgtg ccggtcgagg ccatagttgc
      781 cgccattttg cgcccgatat gtcccgactt gcccatgccc gtgataacga ccctgccctt
      841 gcagtgcaac aacgcgtctg ccgcaaggac gaagtttttg tccaattccg ctgcaatttc
      901 gcgcaagcct tccgcttcgg cgtgcaacac ttcgcgtgcc cagtcgagat attttccgtt
      961 ttctgccatt gccatccgcc tttgctgtaa taaaactacc caaaatttcc gaatcggggt
     1021 agcataagcc ctcaaacccc gcctatccat ccaaaggaat cagagatgac cattttatcg
     1081 gacgttaaag cattaggaca acaaatctgg ctggacaacc tttcccgctc gctagtgcaa
     1141 agcggcgaat tggcgcaaat gttgaaacag ggcgtgtgcg gcgtaacttc caatcccgcc
     1201 atcttccaaa aagccttcgc cggcgacgcg ctttacgccg atgaaatcgc cgccctcaag
     1261 cagcaaaacc tcagccccaa acaacgctac gaaacaatgg cggttgccga tgtgcgtgcc
     1321 gcctgcgacg tttgccttgc cgaacacgaa tccaccggcg gcaaaaccgg cttcgtcagc
     1381 ctcgaagttt cgcccgaatt gtccaaagac gcgcaaggca cggttgaaga agcgcgccgc
     1441 ctctacgccg ccatcgggtg caaaaacgcg atgattaaag tgcctgccac cgacgcaggc
     1501 atcgatgcgc tcgaaaccct cgtttcagac ggcatcagcg tgaacctgac cctgctgttc
     1561 tcacgcgccc aaaccctcaa agcctacgcc gcctacgcgc gcggcatcgc caaacgctta
     1621 gcagccggac aaagcgttgc ccatatccaa gttgtcgcca gcttcttcat ctcccgtgtg
     1681 gacggcgcgc tggacacaac actgcccgac cacctcaaag gcaaaatcgc catcgccctt
     1741 gccaaagccg cctaccaaga ctgggcgcaa tacttcggca gccccgaatt tgccgcgctg
     1801 gaaaccaaag gcgcaaaccg cgtgcagctt ttatgggcat ctaccggcgt gaaaaacccg
     1861 gcctatcccg acacgctcta cgttgacagc ctgatcggcg cgcacaccgt caacaccgtt
     1921 cccgatgcca cgctcaaagc ctttatcgac cacggcacag ccaaagccac gctgaccgaa
     1981 ggcgtagaag aagcacaagc gcagctcgcc gaaaccgccg cgctcggcat cgatgtcgaa
     2041 accttggcaa cgcgtttgca ggaagacggt ttgaaacagt ttgaagaagc gtttgaaaaa
     2101 ctgctcgcgc ctttggctta ggcattttcc cgatgccgtc tgaaaggctt tcagacggca
     2161 tcgcccgttt gaccggacac agtgcggcat atcaaggatt caccctaaaa atattaatgg
     2221 gcaagaagtt aatgcacgac attacccact aacacaaaag gaaatagaat catggcaaaa
     2281 gcaatcgaaa tcatttcacc caatgaaatt tattcagatc tgatttttaa ggatccggta
     2341 cctccccata ct
//
LOCUS       DAXHNE010000204         2352 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.204, whole genome shotgun sequence.
ACCESSION   DAXHNE010000204 DAXHNE010000000
VERSION     DAXHNE010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.204"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            125..2005
                     /locus_tag="ACFM4Z_001984"
     CDS             125..2005
                     /locus_tag="ACFM4Z_001984"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HFC6021956.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTQHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLDNGTKRVGIHIAAPS
                     LAIKQGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAHRPAVSIYFDVDGE
                     FNVGAPTCKIEAVNISENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDEEGKVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPAGKVRMSTKSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGISELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
BASE COUNT          617 a          713 c          569 g          453 t
ORIGIN      
        1 tgttaatcca ctataacgca agcaccgcaa agacgcgcca accctctccc aacctttttt
       61 cagacggcat tttcggtaat ctgctaaaat cgcccgcttg agtttccaca gaaaaatccg
      121 aaaaatgaat attttttacg aagagtccgg ccaattcaaa atcgccgcca tcatccaaaa
      181 aaacgatgcc acctaccaag tcgataccca acacggcaag cgcaccaaag tgaaggcaaa
      241 caatgtcttt gccgagtttg acggcgatat ggcggcgttt ttggaaaacg cgcaggcaca
      301 ggcggcggac atcgacaccg atttattgtg ggaagtatgc ggcgaagagg aatttaccgc
      361 cgaagccatc gccgaagaat attacggcca tgcgccgacc aaaaccgagc tggcggcaac
      421 tttgattgcg ctttacgccg cgccgatgta tttctacaaa aaagccaaag gcgtgttcaa
      481 agccgcgccc gaagaaactt taaaacaagc acttgccgcc atcgaacgca aaaaacagca
      541 agacgcgcaa atcgacgctt gggcggaagc cttgaaacgc ggcgagatgc cgtctgaaat
      601 cgcggcggat ttgaaaacca tcctgcacgc gcccgacaag cagtcgctga cctacaaagc
      661 ctttaccaaa gccgccgacg cgctgaaaac ctctgcctac gaattggcga aaaaaacggg
      721 cggcattacg tccattcccc aatacctgca agacgggttt gaaatcaaat acttccccaa
      781 aggaacaggc tttcccgacc ttccccttcc cgaaatgccc gacctgccca aagccgatgt
      841 taccgccttt tccattgacg acgaatcgac caccgaagtg gacgacgctt taagcctgac
      901 cgacttggac aacggcacga agcgcgtcgg catccacatc gccgcgccgt cgcttgccat
      961 taaacaaggc gacaaaatgg aaaaaaacat catggaacgc ttgagcacgg tttatttccc
     1021 cggcggcaaa atcacgatgc tgcccgaaaa ctggattgcc gcgttcagcc ttgatgcagg
     1081 cgcacaccgc cctgccgtca gcatttattt cgatgtggac ggcgagttca acgtcggcgc
     1141 gccgacctgc aaaatcgaag cggtcaacat ctccgaaaac ctgcgtatcc aagccatcga
     1201 accgcatttc aacgccgaag cgggcttgga cgaagcgggc gaaatgatgt tcgcccacca
     1261 tcaagacctg atttggttct atcaattcgc caccgccctg caaaaagcgc gcggcaaata
     1321 cgaacccgac cgcgcgccgc aatacgatta cagcatcgaa ttggatgagg aaggcaaggt
     1381 atccgtcgtc cgccgcgaac gcggttcgcc catcgatacg ctggtcagcg agatgatgat
     1441 tcttgccaac agcacttggg cgcaaatgct ccatgacaac gacctgcccg gccttttccg
     1501 cgtccaaccg gcaggcaaag tgcgcatgag cactaaatcc gagccgcaca tcggcatggg
     1561 cgtgcagcat tacggctggt tcacttcgcc gctgcgccgc gccgccgact acatcaacca
     1621 aaagcagctg atcagcctga tagacgacac tgccgagccg ctgtatcaaa acagcgatgc
     1681 cgagcttttc gccgcgctgc gcgactttga tgccgcctat accgcctacg ccgacttcca
     1741 acgccaaatg gaagcctact ggagcctggt ttacctgcaa cagcaaggca tcagcgagct
     1801 gacggcgacg attctgaaag aagacctcgt ccgcatcgaa ggcctgccgc tggtcacgcg
     1861 cgcgaccggt attccgtttg acgcactgcc caaatcgcag gcattgttca aaatcaccga
     1921 attagatgcc gagaagcagt ttgtctcgct caactacatc aaggcagtcg cacccgcggg
     1981 tacaacggca ggcaatgccg tctgaagccc gatacggcaa actccggcaa aaacagaaac
     2041 ctgaatctca tcattcccac agtcgggaat ccggtttttt gggtttccgt tgtttttccg
     2101 tttcaatgaa cttccaagcc gccgttcccg ataaataccc gcaatctaaa atcccgtcat
     2161 tcccacgcag atgggaatct aggtctgtcc gcacgggaac ttatgcgccg tcattcccgc
     2221 gaaagcggaa atccagaccg ttcggcttca gttttttggt ttcgggcaac ttctgaatcg
     2281 tcattcccgc gcaggtggaa atataggtct gtccgcacgg gaacttatac gccgtcattc
     2341 ccgcgaaagc gg
//
LOCUS       DAXHNE010000205         2339 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.205, whole genome shotgun sequence.
ACCESSION   DAXHNE010000205 DAXHNE010000000
VERSION     DAXHNE010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.205"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..328
                     /locus_tag="ACFM4Z_001985"
     CDS             <1..328
                     /locus_tag="ACFM4Z_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFC6021957.1"
                     /translation="IATTVPAQQAFLDAAELMQWSIETEGLGLNVISHKILGKDHAQV
                     EFEAYFRDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPAMKQPCICGSGKKFKACCGK
                     YLKPVA"
     gene            complement(547..1341)
                     /locus_tag="ACFM4Z_001986"
     CDS             complement(547..1341)
                     /locus_tag="ACFM4Z_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC6021958.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDSPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            1445..2170
                     /locus_tag="ACFM4Z_001987"
     CDS             1445..2170
                     /locus_tag="ACFM4Z_001987"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFC6021959.1"
                     /translation="MPRFHLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISLGDRMDFTLQKSVELGIT
                     AIQPIISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYRKALDKM
                     PSESTKLIMSINCACKLADIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          622 a          746 c          516 g          455 t
ORIGIN      
        1 catcgctact accgttccgg cgcaacaggc attcctcgat gccgccgaac tgatgcaatg
       61 gagtatagaa accgaagggc tgggcttgaa cgtcatctcg cacaagatac tcggcaaaga
      121 ccacgcccaa gtcgaatttg aagcctactt ccgagacgga caacaccgat ccgcgcatca
      181 cgaactgtcc ggcttcgtca acatcggcgg acaatggtat tttatcgatc ccaccgttcc
      241 gcatcctgcg atgaaacaac cctgcatttg cggatcaggc aaaaaattca aagcctgctg
      301 cggcaaatat ctgaaacctg tcgcataaaa atgccgtctg aacgttcaga cggcattttc
      361 aacgtgcaaa aaaaaaccat tcataccaag ggtaagtatg aatggtcaat acattgcggg
      421 aaaacgtctt acttgctgca ctgccgaaaa gggagaaacg gcagcggtaa tcagcggaaa
      481 ggattgtacc cgaattaata ttaagaaacg ttaatcgcga aaatatatta acaaacctgt
      541 tgaaacctat tggttttccc gtatccaccc gacccagcgt tcaaacagct tcggttcgag
      601 cgcggcaacg accgagcgtt tgaacacgtg ttcaccactc caaaacccgt cgccttccaa
      661 agtcgtcagc ctgccgcccg cctcctcgaa aatcaacgcg ccggcggcat aatcccacag
      721 cttctgcccg ccgtgaacat aaacatcata acgcccgcac gccagataac accaatccaa
      781 cgtactgctg cccatactcc gtatcgttcc aaaaggcgcg agcgtactca tacggctgga
      841 aagtttgccc gaacgcagat atttgatttc cacgcccgca atcgcctcat tgagtttttt
      901 atccacgagg cgcaggggca gacgcgtccc gtttaaaaac gccccctgcc cgcgttcggc
      961 ataaaaacat tcgccgctga ctgggttgta gattacgccc aactcggcgc gcccgttgcg
     1021 gacaaacgcc accgataccg caaaatgcgg cagcccgttg acaaaattgt tcgtcccgtc
     1081 tatcggatcg acaatccaca gccccttttc ccccgaatat tgttcccaca aagccgactg
     1141 ttcctgccgc gacatttcct cacccaacat cggactgtcg attaaaagcg gcaacgcggc
     1201 ggcaaaagcc gtctgcgcgg caatgtccgc ctcgctcaac atcgaaccgt cttccttgcg
     1261 gtgagacggc gtattcaaaa aacgcggcat aatttcggtt tgcgcgatat ggcgcacgac
     1321 tttctgcaaa cggtgtaaca cttcctactg tcctcatatt ttgaacttgc ggcgcgcgaa
     1381 cgtataatgt ccgcttccat cacgccgctg cgacggatta taaccgtccg aaccgccaaa
     1441 aactatgccc cgattccacc tgcccgaaaa cctttccgtc ggacaaaccg tcgccctgcc
     1501 cgacaacatc gtccgccacc tcaacgtcct gcgcgtccgc cccaacgaaa acatcaccct
     1561 cttcgacggc aaaggcaagg cacacgccgc acggctgacc gttttggaaa aacgccgcgc
     1621 cgaagccgaa atcctgcacg aagacacaac cgacaacgag tccccgctca acatcacgct
     1681 catccaatcc atttccttag gcgatcgcat ggatttcacc ctgcaaaaaa gcgtcgaact
     1741 cggcataacc gccatacagc ccatcatcag cgaacgctgc atcgtccgcc tcgacggaga
     1801 acgcgccgcc aaacgcctcg cacgctggca ggaaatcgtc atctccgcgt gcgaacagag
     1861 cggcaggaac accgttcccc ccgtactgcc tatcatcggc taccgtaaag cactcgacaa
     1921 aatgccgtct gaaagcacca agctgattat gagcatcaac tgcgcctgca aacttgccga
     1981 catccgccac ccgtccggcg caatcgtctt tatggtcggc cccgaaggcg gctggacaga
     2041 acaggaagaa caacaggcat ttgaagctgg ctttcaggcg gttacactcg gcaaacggat
     2101 tttacgcaca gaaaccgccc cactcgccgc cctcgccgcc atgcagacgc tttggggcga
     2161 tttcgcataa acagaaatgc cgtctgaaac ccgttcagac ggcattttgc agccgattaa
     2221 gatagtaggt tcaaataaga tttcccgtgt cgtcattccc gcgaaagcgg gaatctagaa
     2281 acgaaaaact acagagattt atccgaaaca acaaccctct ccgccgtcat tcccgcaaa
//
LOCUS       DAXHNE010000206         2338 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.206, whole genome shotgun sequence.
ACCESSION   DAXHNE010000206 DAXHNE010000000
VERSION     DAXHNE010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.206"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            77..2227
                     /gene="yccS"
                     /locus_tag="ACFM4Z_001988"
     CDS             77..2227
                     /gene="yccS"
                     /locus_tag="ACFM4Z_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFC6021960.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYEALGSYLEAKADFFDPDEAEWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDSNDSPDIRHLRRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAALETSSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVETLNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKIAERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT          548 a          897 c          475 g          418 t
ORIGIN      
        1 agctaaggcg aggcaacgcc gtactggttt ttgttaatcc actatattca cttcataaca
       61 ataaaccggt aaaaccatga aaaccccact cctcaagcct ctgctcatta cctcgcttcc
      121 cgttttcgcc agtgttttta ccgccgcctc catcgtctgg cagctaggcg aacccaagct
      181 cgccatgccc ttcgtactcg gcatcatcgc cggcggcctt gtcgatttgg acaaccgcct
      241 gaccggacgg ctgaaaaaca tcatcgccac cgtcgccctg tttaccctct cctcgctcac
      301 ggcacaaagc accctcggca cagggctgcc cttcatcctc gccatgaccc tgatgacctt
      361 cggctttacc attttaggcg cggtcgggct gaaataccgc accttcgcct tcggcgcact
      421 cgccgtcgcc acctacacca cacttaccta cacccccgaa acctactggc tgaccaaccc
      481 cttcatgatt ttatgcggca ccgtactgta cagcaccgcc atcatcctgt tccaaatcat
      541 cctgccccac cgccccgttc aagaaagcgt cgccaacgcc tacgaagcgc tcggcagcta
      601 cctcgaagct aaagccgact ttttcgatcc cgacgaagcc gaatggatag gcaaccgcca
      661 catcgacctc gccatgagca acaccggcgt catcaccgcc ttcaaccaat gccgttccgc
      721 cctgttttac cgccttcgcg gcaaacaccg ccacccgcgc accgccaaaa tgctgcgtta
      781 ctactttgcc gcccaagaca tacacgaacg catcagctcc gcccacgtcg attatcagga
      841 aatgtccgaa aaattcaaaa acaccgacat catcttccgc atccaccgcc tgctcgaaat
      901 gcagggacaa gcctgccgca acaccgccca agccctgcgc gcaagcaaag actacgttta
      961 cagcaaacgc ctcggccgcg ccatcgaagg ctgccgccaa tcgctgcgcc tcctttcaga
     1021 cagcaacgac agtcccgaca tccgccacct gcgccgcctt ctcgacaacc tcggcagcgt
     1081 cgaccagcag ttccgccaac tccagcacaa cggcctgcag gcagaaaacg accgcatggg
     1141 cgacacccgc atcgccgccc tcgaaaccag cagcctcaaa aacacctggc aggcaatccg
     1201 tccgcagcta aacctcgaat caggcgtatt ccgccatgcc gtccgcctgt ccctcgtcgt
     1261 tgccgccgcc tgcaccatcg tcgaaaccct caacctcaac ctcggctact ggatactgct
     1321 gaccgccctt ttcgtctgcc agcctaacta caccgccacc aaaagccgcg tccgccagcg
     1381 cattgccggt accgtactcg gcgtaatcgt cggctcgctc gtcccctact tcaccccgtc
     1441 tgtcgaaacc aaactctgga ttgtcatcgc cagtaccacc ctctttttca tgacccgcac
     1501 ctacaaatac agtttctcca ccttcttcat taccattcaa gccctgacca gcctctccct
     1561 cgcaggtttg gacgtatacg ccgccatgcc cgtacgcatc atcgacacca ttatcggcgc
     1621 atcccttgcc tgggcggcag tcagctacct atggccagac tggaaatacc tcacgctcga
     1681 acgcaccgcc gcccttgccg tatgcagcaa cggcgcctat ctcgaaaaaa tcgccgaacg
     1741 cctcaaaagc ggcgaaaccg gcgacgacgt cgaataccgc gccacccgcc gccgcgccca
     1801 cgaacacacc gccgccctca gcagcaccct ttccgacatg agcagcgaac ccgcaaaatt
     1861 cgccgacagc ctgcaacccg gctttaccct gctcaaaacc ggctacgccc tgaccggcta
     1921 catctccgcc ctcggcgcat accgcagcga aatgcacgaa gaatgcagcc ccgactttac
     1981 cgcacagttc cacctcgccg ccgaacacac cgcccacatc ttccaacacc tgcccgaaac
     2041 cgaacccgac gactttcaga cagcactgga tacactgcgc ggcgaactcg acaccctccg
     2101 cacccacagc agcggaacac aaagccacat cctcctccaa cagctccaac tcatcgctcg
     2161 gcagctcgaa ccctactacc gcgcctaccg acaaattccg cacaggcagc cccaaaacgc
     2221 agcctgaaaa agtttcggca ttttgtaaga gagggcagat tgtcagacag gttgcaagat
     2281 agtggataaa gctttgcgca gggtaaatgc gtagcaactg aaccgtcatt cccacgaa
//
LOCUS       DAXHNE010000207         2298 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.207, whole genome shotgun sequence.
ACCESSION   DAXHNE010000207 DAXHNE010000000
VERSION     DAXHNE010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.207"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(36..992)
                     /locus_tag="ACFM4Z_001989"
     CDS             complement(36..992)
                     /locus_tag="ACFM4Z_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6021961.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGDLADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRPFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            1228..2193
                     /locus_tag="ACFM4Z_001990"
     CDS             1228..2193
                     /locus_tag="ACFM4Z_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6021962.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGARQLIQAAEQLKAAFEKAEPVAAGKE"
BASE COUNT          489 a          612 c          733 g          464 t
ORIGIN      
        1 tgtttcaaat caaaatatct atgccgccgg ccccatcagc acttcttcac atccgaaggc
       61 aaaaatccgt aatgccgtct gaacgcttcg ttgaaccgtc ccgcgtggcg gtagccgcaa
      121 aagtgcatgg cggcttggac ggtgctgccg gattcgatga gggcgagcgc gtgttccagc
      181 cgcaggcggc gcaggcatcc ggcgacggtt tcgccggttt gcgctttgaa atagcgtttc
      241 aggtagcatt cgttcagtcc gacgcggcgg gcgatttcgg cgatggtcag cggacgggcg
      301 aattcgtgtt gcaggatgtc ggcggcttcg tctatgcgcc gacggcggta accgttgtcg
      361 tggcggcgga aggtgaagcg caataatcgg gcggagagtt ccagcgcggc ggcttcgtcg
      421 gcaagcaggc cgaagccgtc cgattcgaac gggcgttgca gcagtgggca ggccgccgcc
      481 gtcagtgccg ctgcgttttg cgccagccgt tgcagggcga atcggcctat tgtttgcggc
      541 gaaaacaggc gttcgtccag caagccttcg tcgtgccagc ggcgcagttt ttccagcgaa
      601 aaatccaaat gcagcgcgca catgccgctg ttgtcgggca gcagggtttc ggatacgtcc
      661 gccaaatcgc cgcgtaccag ccagatttcg ccggcagatg ggcggtattc cctgccgccc
      721 atttgtaacc ggttctgccc cgacaccatg acgaacaagg cgcagttgtg gctgaaattg
      781 tggatttcgg tgggaaacgc gcccgttccg ccgccgcgca tccgcgacaa ggtgatgccc
      841 gagtcgaagc ggttgatgca catttccaga tgcaaaccgg gctgttttgc ctgcgcaatg
      901 agcgcgctgt cggaacagcc gtccaacgcc cagccggatt tatcggagcg gacataggtt
      961 tggtactggc ggtagatggc ggcggtgttc atgattggat aggaacgagt tgtctaacaa
     1021 atgaattaaa taggaattat taccaataat caagcgcagg gattggttga aacggaaaag
     1081 gtcgtctgaa agggtgtttc agacgacctt ttccgtatcg ggaatttgtt ttgccgtatc
     1141 gggaattttg cgttttgcgg cgtggtttct gcaggttgtt tgcttaataa taaacattct
     1201 tattcgtatg caaaggaacc gcacaccgtg aaaccgcgtt tttattgggc agcctgcgcc
     1261 gtcctgctga ccgcctgttc gcccgaacct gccgccgaaa aaactgtatc cgccgcatcc
     1321 gcatctgccg ccacactgac cgtgccgacc gcgcggggcg atgccgttgt gccgaagaat
     1381 cccgaacgcg tcgccgtgta cgactgggcg gcgttggata cgctgaccga attgggcgtg
     1441 aatgtgggcg caaccaccgc gccggtgcgc gtggattatt tgcagcctgc atttgacaag
     1501 gcggcaacgg tggggacgct gttcgagccc gattacgaag ccctgcaccg ctacaatcct
     1561 cagcttgtca ttaccggcgg gccgggcgcg gaagcatatg aacagttggc gaaaaacgcg
     1621 accaccatag atctgacggt ggacaacggc aatatccgca ccagcggcga aaagcagatg
     1681 gagaccttgg cgcggatttt cggcaaggaa gcgcgcgcgg cggaattgaa ggcgcagatt
     1741 gacgcgctgt tcgcccaaac gcgcgaagcc gccaaaggca aaggacgcgg gctggtgctg
     1801 tcggttacgg gcaacaaggt gtccgccttc ggcacgcagt cgcggttggc aagttggata
     1861 cacggcgaca tcggcctacc gcctgtagac gaatctttac gcaacgaggg gcacgggcag
     1921 cctgtttcct tcgaatacat caaagagaaa aaccccgatt ggattttcat catcgaccgt
     1981 accgccgcca tcgggcagga agggccggcg gctgtcgaag tattggataa cgcgctggta
     2041 cgcggcacga acgcttggaa gcgcaagcaa atcatcgtca tgcctgccgc gaactacatt
     2101 gtcgcgggcg gcgcgcggca gttgattcag gcggcggagc agttgaaggc ggcgtttgaa
     2161 aaggcagaac ccgttgcggc ggggaaagag tagggagttg tctgaaaacg gagcttccga
     2221 aggaagcggg ggggtttctg cgaagctaaa gtacggtttc aacgaattga aaagcagcct
     2281 gtatgttgaa aataccgc
//
LOCUS       DAXHNE010000208         2282 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.208, whole genome shotgun sequence.
ACCESSION   DAXHNE010000208 DAXHNE010000000
VERSION     DAXHNE010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.208"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(27..1436)
                     /gene="dacB"
                     /locus_tag="ACFM4Z_001991"
     CDS             complement(27..1436)
                     /gene="dacB"
                     /locus_tag="ACFM4Z_001991"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HFC6021963.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTINDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLQITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGIADTPEG
                     AQTLAVAHAKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMVVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1601..2167
                     /locus_tag="ACFM4Z_001992"
     CDS             1601..2167
                     /locus_tag="ACFM4Z_001992"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFC6021964.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTVFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          520 a          558 c          660 g          544 t
ORIGIN      
        1 aatttaatcc actatatttt cctgtttcag gctcggcgtt ctttgcacat cagtttcgca
       61 tccagccagc catcgccgcc ggagatgatg ttgttggcaa cgaagttgtc caagtcgggc
      121 agcagggaaa cggcgcgacc gctgttgatg atgacgacca ccaccatcgg tttgtcgccc
      181 agccagtagc ctgccaatgc gcggacattg ttgagcgtgc cggttttcag gcgcagaagc
      241 ccaccgcttt gtttgaagcg gttgcgtaaa gttccgtctg tgccggcgat gggcagcgtg
      301 tcgatgaaat cttgtgcaaa cgggctgaaa taagccgttt ccaacatttg cgccatcatt
      361 ctcgccgtta ccctttcttt tctagacaga cccgaaccgt tttccaaaac caaatccgca
      421 acatcgatgc ccgacacggc aagttcgcgc cggacggcag acgctgcctg ttcggaaacg
      481 gcgggcagtt tgccgtcgcc gccgagtttg aggaagacgg aacgcgcaat tagattgtcc
      541 gaacgcttgt tcatgtccgt caaaatttcc ttcatcggtt tggcgtgtgc aacggcaagc
      601 gtctgcgcgc cttccggcgt gtcggctatg ccgataccgt ctgaaatccg tccgccgccg
      661 agcagccagt ggttggtaaa actttgccgg atcagttcgt caagcgcgaa catccggaca
      721 ccgacaggct tgcccaaaca gctctcggga atattgccgc gcaatttcag cgtattgtcc
      781 gaaaaagatg cacgcatcag ttttttgatc gaagggcagg cagcttggga ggcggtaatt
      841 tgcaagttgt tttgggcgaa aatatgcggc aaaggcggat cggtgaggat gtcggtactg
      901 tcggcggcat tgcgttcggc gcgcaccata accataccgg cagacagcat agttggattg
      961 gggggcgtca taaacggcga accgctgtcg gcttcgaaat cgtcggggct gccgacttcg
     1021 ccccacaggc tgtggtcgag catcaggtgt cccgtgatat tgcgtatgcc ttgttcgcgc
     1081 aactggcgtt ggacggcaag caggttttcc tgattgaaaa cggggtcgcc gctgcccgcc
     1141 caatacaggt ttccgtcaag cgtgccgtcg tttatcgtac cgttgctttt aaactcggtc
     1201 gcccagcggt aattgctgcc gaaggttttg aaggcggcaa acgcggtaac gagtttcatt
     1261 gtggaggcgg ggttgacggg gacatccgag cggtggtcaa tgatgacttt tccgctgtca
     1321 agctcttgga catatacggc gatttcgttt tgcggaatgc gacccgtatc gagcgcgtgt
     1381 gcggcgaggg aggcgagaag cagcagcagg gaggccgctg ttttggggaa attcataggt
     1441 aaatctgtta cataaaaaaa gcaattataa ggcaaagccg gataagtggg aacgctgtgg
     1501 cggcgggccg gcttgtttgc aggggaaatc acatatataa taatcgctat cattatgaaa
     1561 tgattgagac gcacaaactt aatattcagg aggaatgatt atggctaaga aaatcagtat
     1621 tttggtgggc agcctgcgcc gtgcttcttt tgcgcgcaaa gtggcattga atgcggcgga
     1681 gatgttcccc gaaggctggc aggcggaaat cgtcgaaatc ggacatctgc cgctttacaa
     1741 tttcgattat gacgaccctg cggtggaaga tgtgccgctg cccgaaagct acacggtttt
     1801 ccgcgaaacg attaaggctt cggacggcat tttgttcgtt acgtccgaaa ataaccgcac
     1861 cattcccgcc tgtttgaaaa atgcggtgga catcggctcg aaaccgaatg ccgacgtggc
     1921 gtggaaaaac aaaccggccg gcatcatcag ccattccgtc ggcaagatgg gcggttacag
     1981 ttcgcaaaaa aacctgcgcc ttgcgctgtc ttatttcgat atgcccgtaa ccggacagcc
     2041 ggaagtgttt ttgggcaatt cgccgacact gtttgatgaa aacggcaaat tgatcgactc
     2101 ggcaagggat tttgttcagt catatattaa tcagtttgtc ggtttgattg aaagaaatgc
     2161 caaataaaca aacaagtcga aaggccgcaa aaccggttcg ggttttgcgg cttttttata
     2221 tgggctgggc agcgtcaagg tttgccgttt gccggataat gcgcggcaaa tgaccggcgt
     2281 gt
//
LOCUS       DAXHNE010000209         2254 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.209, whole genome shotgun sequence.
ACCESSION   DAXHNE010000209 DAXHNE010000000
VERSION     DAXHNE010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.209"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..567)
                     /locus_tag="ACFM4Z_001993"
     CDS             complement(<1..567)
                     /locus_tag="ACFM4Z_001993"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFC6021965.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPSGELAAAIDAKWG
                     SFEKFQEAFSACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAK"
     gene            730..2136
                     /gene="dnaB"
                     /locus_tag="ACFM4Z_001994"
     CDS             730..2136
                     /gene="dnaB"
                     /locus_tag="ACFM4Z_001994"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFC6021966.1"
                     /translation="MNDYTAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAFNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          545 a          585 c          604 g          520 t
ORIGIN      
        1 tttggcgact tcgtcccagt tgacgatttc ccaaaaacct ttcaggtagt tggggcggct
       61 gttgcggtag tcgatgtagt aggcgtgttc ccacacgtcg caggtcagca gcggcgtgtt
      121 ttcagtggtc agcggcgtag cggcgttgga agtggaaacc aaatccaatc cgccggcagg
      181 ggttttcacc agccacgccc aaccggagcc gaaagtaccg gcagcgcaag cggagaatgc
      241 ttcttggaat ttctcgaagc tgccccattt cgcgtcgatg gcggcggcca gttcgccgga
      301 aggtttgcct tgacctttgg gcgtgaaacc cagccagtag aaagtgtggt tccaagtttg
      361 ggctgcgttg ttgaacacgc cgcccgaaga ttttttcaca atctcttcca aaggcaggtt
      421 ttcaaattcg gtgcctttga tttgattgtt caggttggtg atgtaggttt gatggtgttt
      481 gccgtagtgg aattccaaag tttctttgct cagatgcggg gacaatgcgt ccagttcata
      541 aggcagttgc ggcagcttat gttccatttt gtgctcctaa tattgtttta aatgttgtat
      601 tttggcagtg ttgctgcaaa tagctcagca gcgcacatat tctacctgtt ttgaggtacg
      661 gaaaccaatt aaacctgctt tacgctataa tagaagattg caatttcggc acgacagata
      721 ggatgtacca tgaacgatta cacagctatg ccgtctgaag accgtgaggt cggcgcattg
      781 tcgctgcctc cacattcgat ggaggcggaa caatccgttt tgggcgggtt gatgctggaa
      841 aatccggcct gggacaggat tgccgatgtg gtttcgggag aggatttcta ccgccacgaa
      901 caccgcctga ttttccgatc cattgccaaa ttaatcaatg agagccgtcc cgccgatgtg
      961 attacggttc aggaagattt gcagcggaac gaagaattgg aagcggcagg cggattcgaa
     1021 tacctgatta cgctggcgca aaacaccccg tctgccgcca acatccgccg ctatgccgaa
     1081 atcgtgcgcg agcgttccat tatgcgccaa ctcgccgaag tggggacgga aatcgcccgc
     1141 agcgcattca atccgcaagg cagggacgcg gggcagcttt tggacgaggc ggaaaacaaa
     1201 gtattccaaa tcgccgaaag cacggccaaa tccaagcagg gctttttgga gatgcccgat
     1261 ttgctgaaag aagtcgtaca gcgcatcgat atgctctact cgcgcgacaa tcccgatgaa
     1321 gttaccggcg tgccgacagg gtttatcgac ctcgacaaaa aaacctcggg ccttcagccc
     1381 ggcgacctga ttattgttgc cggtcgtccg tctatgggta agaccgcctt ttccatcaat
     1441 atcgccgaac acgttgccgt agaaggcaag ctgccggttg ccgtcttctc aatggagatg
     1501 ggcggggcgc aactggtcat gcgtatgctc ggctcggtcg gacgcttgga tcaaagcgtt
     1561 ttgaaaaccg gcaggctcga agacgaacac tggggtcgcc tgaacgaagc agtcgtcaaa
     1621 ctctccgacg cgcccgtgta catcgacgag accccgggtc tgaccgcgct cgaactgcgc
     1681 gcccgtgccc gccgtctcgc ccgtcaattt aacaataagc tgggattaat cgtcatcgac
     1741 tacctgcaac tgatggcagg atccggccgt tccgacaacc gagcttcaga gctgggcgaa
     1801 atttcacgtt cgctcaaagc gttggcgaaa gaattgcaag tccccatcat cgccctgtcg
     1861 caattgagcc gtaccgtcga atcgcgtacc gacaaacgcc cgatgatgtc cgacctccgc
     1921 gaatccggcg caatcgagca agatgccgac ctgattatgt tcatgtaccg cgacgaatac
     1981 tacaaccagg actcacccat gaaaggcctt gccgaatgta tcatcggcaa acaccgcaac
     2041 ggtcccgtcg gtaaaatctt cctcacatgg acgggacaat tcaccaaatt cgacaatgct
     2101 gcctatattc ccgaggaggc aaagatagaa gattaaatgg ctatataaaa atttattagg
     2161 cgaaatcagg caaaatcgtt taaaatcatg ctgagagatt gccctaaaaa ataaaacgcg
     2221 gtcttgaggc atttttgcat tcagcccgca tata
//
LOCUS       DAXHNE010000210         2200 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.210, whole genome shotgun sequence.
ACCESSION   DAXHNE010000210 DAXHNE010000000
VERSION     DAXHNE010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.210"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..>2200
                     /gene="pilC"
                     /locus_tag="ACFM4Z_001995"
     CDS             <1..>2200
                     /gene="pilC"
                     /locus_tag="ACFM4Z_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC6021967.1"
                     /translation="AQTYKYAIIMNEGNQLEVKGNGQYSTIKDKDREREYTYHQGRGG
                     GGSVSFNNSDELVSRQSGTAVFGTATYLPPYGKVSGFDERKLKERTNALNWIHTTHPG
                     LIGYSYTGVVCRDSTGCPKLVYKTQFTFGNSGLAKKANGGGLDIYVDKSRDNSPIYKL
                     KDHPWLGVSFNLGSENTVKNSKSSNKLISSFSEDNNNQTIVSTTEGSPISLGDGKREH
                     TAMAYYLNAKLHLLDKKGIKDITNKTVQLGVLRPSIDVRLQGNTGLGGLLAFHATWDI
                     KDTGQILVKLGLPQVKAGRCINKPNPNPNAQAPSPALTAPALWFGAGQNGKVQMYSAS
                     VSTYPDSSSSRIYLQNLKRKTDPGRPGRYSLADLTKSDIESRQPGFTGRQTVIRLPGG
                     VYKINSGKNGGRVAGINGNDGKNDTFGILKDRYVTPETNEWSEVLLPWTVRGSADDNR
                     FKTINQDSAKYSQRYRIRNTSNGERNLGDIVNSPIVAVGGYLATSANDGMVHIFKKNG
                     GSDERSYNLKLSYIPGTMPRKDIENTESTLAKELRTFAEKGYVGDRYGVDGGFVLRQV
                     ERNGKDHVFMFGAMGFGGRGAYALDLSKIDSNNPTAVSLFDVKHDNSGNNGNNGNNRV
                     ELGYTVGTPQIGKTHNGKYAAFLASGYATKDINNGENKTALYVYDLESSGTLIKKIDV
                     PGGKGGLSSPTLVDKDLDGTVDIAYAGDRGGSMYRFDLKDWSVRTIFQGT"
BASE COUNT          618 a          574 c          562 g          446 t
ORIGIN      
        1 tggcgcaaac ctataaatac gctattatca tgaacgaggg aaaccagctc gaggtaaagg
       61 ggaatgggca atattcaaca ataaaggaca aagacaggga acgcgaatat acttatcatc
      121 agggcagagg aggaggaggc tctgtctcat tcaacaatag cgatgagctt gtttctcgac
      181 aaagcggtac tgccgttttt ggcacagcca cctacctgcc gccctacggc aaggtttccg
      241 gttttgatga aagaaagctg aaagagcgca ccaatgccct taattggatt catacgaccc
      301 acccagggtt gataggctac agctacaccg gtgtcgtatg cagagacagc acaggctgtc
      361 ccaaacttgt ctataaaacc caatttacct tcggtaattc cggtttggca aaaaaggcaa
      421 acggcggcgg gctggatata tacgtagaca aaagccgcga caattcgccc atttacaaat
      481 tgaaagatca tccatggttg ggcgtatctt tcaatttggg cagcgagaat accgtcaaaa
      541 atagcaaatc atccaacaaa ttgatatctt cttttagtga agacaataat aatcaaacca
      601 tcgtctctac gacagaaggc tcccctattt cccttggcga cgggaagcgc gaacataccg
      661 ccatggccta ttatctgaac gccaaactgc acctgctgga caaaaaaggg attaaagata
      721 tcaccaacaa aacagtgcag ttgggtgtct tgaggccgag catcgatgtg cggctgcaag
      781 gaaatacggg gcttggcggc ttgctagctt ttcatgctac gtgggacatt aaagataccg
      841 ggcagattct ggtcaagctc ggcctgccgc aagtcaaagc aggccgctgc atcaataaac
      901 cgaaccccaa tcccaacgcc caagccccct cccccgcact gactgccccc gcgctgtggt
      961 tcggagctgg gcaaaatggc aaggtgcaga tgtattccgc ttcggtttcc acctaccccg
     1021 acagttcgag cagccgcatc taccttcaaa atctgaaaag aaaaaccgac cccggcagac
     1081 ccggccgcta ttctctcgca gacttgacta agtcggatat tgaaagtcga cagccgggtt
     1141 tcacagggcg gcagaccgtc atccgattgc cgggcggcgt gtacaagatc aactcgggca
     1201 agaacggcgg ccgggtcgcg ggtattaatg gcaatgacgg caaaaacgac actttcggca
     1261 tcctcaagga caggtatgtc acacctgaga ccaacgagtg gagcgaagtg ctgctgcctt
     1321 ggacggttcg gggttctgct gatgacaatc gatttaaaac aattaaccaa gactcagcaa
     1381 aatacagcca aagataccgc atccgcaaca ccagcaatgg cgaacgcaat ttaggcgaca
     1441 tcgtcaacag cccgattgtc gcggtcggcg ggtatttggc tacttccgcc aacgacggga
     1501 tggtgcatat cttcaaaaaa aacggcggca gtgatgaacg cagctacaat ctgaagctca
     1561 gctacatccc cggcacgatg ccgcgcaagg atattgaaaa caccgaatcc acccttgcca
     1621 aagagctgcg cacctttgcc gaaaaaggct atgtgggcga ccgctacggc gtggacggcg
     1681 gctttgtctt gcgccaagtc gaacggaatg ggaaagacca tgtgtttatg ttcggcgcga
     1741 tgggctttgg cggcagaggc gcatacgcct tggatttaag caaaatcgac agcaataacc
     1801 cgaccgccgt ttccctgttt gatgtcaaac atgacaatag tggcaataat ggcaataacg
     1861 gcaataatcg cgtggaatta ggctacaccg tcggcacgcc gcaaatcggc aaaacccaca
     1921 acggcaaata cgccgccttc ctcgcctccg gttatgcgac taaagacatt aacaacggcg
     1981 agaataaaac cgcgctgtat gtgtatgatt tggaaagcag cggcacgctg attaaaaaaa
     2041 tcgatgtacc cggcggcaag ggcgggcttt cgtcccccac gctggtggat aaagatttgg
     2101 acggcacggt cgatatcgcc tatgccggcg atcgcggcgg cagtatgtac cgctttgatt
     2161 tgaaagactg gtctgtacgc actattttcc aaggcacgaa
//
LOCUS       DAXHNE010000211         2164 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.211, whole genome shotgun sequence.
ACCESSION   DAXHNE010000211 DAXHNE010000000
VERSION     DAXHNE010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.211"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            39..523
                     /locus_tag="ACFM4Z_001996"
                     /pseudo
     CDS             39..523
                     /locus_tag="ACFM4Z_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            619..1242
                     /gene="rsmG"
                     /locus_tag="ACFM4Z_001997"
     CDS             619..1242
                     /gene="rsmG"
                     /locus_tag="ACFM4Z_001997"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFC6021968.1"
                     /translation="MERKERLRAGIAAMGLDISETAQDRLLVYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTVHLLKDG
                     GYWAAMKGVYPQEEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            1341..2114
                     /locus_tag="ACFM4Z_001998"
     CDS             1341..2114
                     /locus_tag="ACFM4Z_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC6021969.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVQSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVEEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELAARVSGK"
BASE COUNT          415 a          497 c          754 g          498 t
ORIGIN      
        1 ctatataatg cggtctgctt cggaagaggg ggacggcgat gtttgtgaac gagaaatatc
       61 cttatgcggc tctgtttgcg ggactggtgt ttttgacgct gccgtttgcg ttggcggtgc
      121 atgatgcctt tgcgcttgcg ttcggacgga cggggttgct ggtgtcggtg tcggacggcg
      181 gattcggctg gcgtggcggt tgggacggca ctgtttggtt tgtgttcggt gtgtttgcgt
      241 ttttgaatgt ggttgtgtcg gcgggtctga cgaaactggc gtacaaaaag atgatgcggc
      301 ggcattcgcg ttacacactg tttctgtcgg gcgtggcggc ttgcgcggcg gcagcggtgg
      361 cttggatttt cgagctgctg cttggcagtg gggctttggg cgggctgcgg gggaggcggt
      421 gttggaatat gcgtttgccg tgtggctggt ggcgatgctg acgctgccca aacgcctgac
      481 gcgcgcgccg gtgcagccgg tggtgtttca caggaaaaaa taggttggaa cgggaaatgc
      541 cgtctgaaac ccgacacgcg gtttcagacg gcatgttttt ccgctaacat tacgcctgaa
      601 tatggacagg aagcagatat ggaacgcaaa gaacgcctgc gtgcaggcat tgccgcgatg
      661 gggctggata tttcggaaac ggcgcaggac aggcttttgg tctatgtgga tttgttgaaa
      721 aagtggaaca aaacctacaa tctgaccgcc ctgcgcgacg aggaaaaaat gattgtccat
      781 catcttttgg acagccttac gctgctgccc catatcgagg gtgtgcaaac gatgctggat
      841 gtcggttcgg gcggcggtca gcccggcatt ccggcggcgg tgtgccgtcc ggatgtgcaa
      901 ataacccttt tggatgcgaa tacgaagaaa acggcttttt tacagcaggc ggttatcgag
      961 ttggggttgg acaatgtgcg cgtggtatcc ggacgcgtgg aggcggtttc ggacgtgcgt
     1021 gccgatgtgg ttaccagccg tgcgtttgca gaactggcgg attttgtgtc gtggacggtg
     1081 catctgttga aagacggcgg ctactgggcg gcgatgaagg gcgtgtatcc gcaggaagaa
     1141 atcggccgcc tgccgcagga tgtgtgcgtt gaaaaagtcc aaaggctcga cgtgccgggc
     1201 ttggatgcgg aacgccatat cgtcatcctg agcaagcgtt gagcgcactt cagacggcat
     1261 gaataccttt tttgtgcgga taaaggtaaa attccgcact gtttttcttt tttcaacatc
     1321 agacgggaca cgggcgggac atgagtgcga acatccttgc catcgccaat cagaagggcg
     1381 gtgtgggcaa aacgacgacg acggtaaatt tggcggcttc gctggcatcg cgcggcaaac
     1441 gcgtgctggt ggtcgatttg gatccgcagg gcaatgcgac gacgggcagc ggcatcgaca
     1501 aggcgggttt gcagtccggc gtttatcagg tcttattggg cgatgcggac gtgcagtcgg
     1561 cggcggtacg cagcaaagag ggcggatacg ctgtgttggg tgcgaaccgc gcgctggccg
     1621 gcgcggaaat cgaactggtg caggaaatcg cccgggaagt gcgtttgaaa aacgcgctca
     1681 aggcagtgga agaagattac gactttatcc tgatcgactg cccgccttcg ctgacgctgt
     1741 tgacgcttaa cgggctggtg gcggcgggcg gcgtgattgt gccgatgttg tgcgaatatt
     1801 acgcgctgga agggatttcc gatttgattg cgaccgtgcg caaaatccgt caggcggtca
     1861 atcccgattt ggacatcacg ggcatcgtgc gcacgatgta cgacagccgc agcaggctgg
     1921 ttgccgaagt cagcgaacag ttgcgcagcc atttcgggga tttgcttttt gaaaccgtca
     1981 tcccgcgcaa tatccgcctt gcggaagcgc cgagccacgg tatgccggtg atggcttacg
     2041 acgcgcaggc aaagggtacc aaggcgtatc ttgccttggc ggacgagctg gcggcgaggg
     2101 tgtcggggaa ataggtcaat ccaaatcggg ctgcccgtgc ctttatgctg tttggccggg
     2161 tgcg
//
LOCUS       DAXHNE010000212         2152 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.212, whole genome shotgun sequence.
ACCESSION   DAXHNE010000212 DAXHNE010000000
VERSION     DAXHNE010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.212"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            123..1508
                     /locus_tag="ACFM4Z_001999"
     CDS             123..1508
                     /locus_tag="ACFM4Z_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFC6021970.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            1528..2046
                     /locus_tag="ACFM4Z_002000"
     CDS             1528..2046
                     /locus_tag="ACFM4Z_002000"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HFC6021971.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTKQAQNGADHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
BASE COUNT          458 a          651 c          578 g          465 t
ORIGIN      
        1 ttcaaataaa caggaatata acttcagaca aacaacttac cgcccgattt gtgctatcgt
       61 tttcgcacaa cttaaaaaaa cctgacaatt ttgtactttt attacagaga aaggctttac
      121 aaatggacgg ctggacacag acgctgtccg cgcaaaccct gttgggcatt tcggcggcgg
      181 caatcatcct cattctgatt ttaatcgtca aattccgcat ccacgcgctg ctgacactgg
      241 tcatcgtcag cctgctgacg gctttggcaa ccggtttgcc cacaggcagc attgtcaacg
      301 acgtactggt caaaaacttc ggcggcacgc tcggcggcgt ggcgcttctg gtcggcctgg
      361 gcgcgatgct cggacgtttg gtcgaaacat ccggcggcgc acagtcgctg gcggacgcgc
      421 tgatccggat gttcggcgaa aaacgcgcac cgttcgcgct gggcgttgcc tcgctgattt
      481 tcggcttccc gattttcttc gatgccggac taatcgtcat gctgcccatc gtgttcgcca
      541 ccgcacggcg catgaaacag gacgtactgc ccttcgcgct tgcctccatc ggcgcatttt
      601 ccgtcatgca cgtcttcctg ccgccccatc cgggcccgat tgccgcttcc gaattttacg
      661 gcgcgaacat cggccaagtt ttgattttgg gtctgccgac cgccttcatc acatggtatt
      721 tcagcggcta tatgctcggc aaagtgttgg ggcgcaccat ccatgttccc gttcccgaac
      781 tgctcagcgg cggcacgcaa gacaacgacc tgccgaaaga acctgccaaa gcaggaacgg
      841 tcgtcgccat catgctgatt cccatgctgc tgattttcct gaataccggc gtatcggccc
      901 tcatcagcga aaaactcgta agtgcggacg aaacctgggt tcagacggca aaaataatcg
      961 gttcgacacc gatcgccctt ctgatttccg tattggtcgc actgtttgtc ttgggacgca
     1021 aacgcggcga aagcggcagc gcgttggaaa aaaccgtgga cggcgcactc gcccccgtct
     1081 gttccgtgat tctgattacc ggcgcgggcg gtatgttcgg cggcgttttg cgcgcttccg
     1141 gcatcggcaa ggcactcgcc gacagcatgg cggatttggg cattcccgtc cttttgggct
     1201 gtttccttgt cgccttggca ctgcgtatcg cgcaaggttc ggcaaccgtc gccctgacca
     1261 ccgccgccgc gctgatggct cctgccgttg ccgccgccgg ctttaccgac tggcagctcg
     1321 cctgtatcgt attggcaacg gcggcaggtt cggtcggttg cagccacttc aacgactccg
     1381 gcttctggct ggtcggccgc ctcttggaca tggacgtacc gaccacgctg aaaacctgga
     1441 cggtcaacca aaccctcatc gcactcatcg gctttgcctt gtccgcactg ctgttcgcca
     1501 tcgtctgaca gacggaaagg atagtaaatg actacgcatt ttgtcgttat gggcgtatgc
     1561 ggctgcggca agaccaccgc cgcgctgtcc ctgcagaaac acctcggtca atgtccctat
     1621 gccgaaggcg acgagttcca cacccaagcc aaccgcgaca agatgggcgc gggtattccg
     1681 ctgaccgatg aagaccgcta tccgtggttg ggcaatctgc gcgactggat gacgaaacag
     1741 gcgcaaaacg gcgcggacca caccatcgta acctgttccg ccctcaaacg cggctaccgc
     1801 gacattctgc gcggagccga aggcaaagct gccttcatcc acctcagtcc gccgcaagac
     1861 atcaacctcg agcgcatgat gtcgcgcaaa ggacattaca tgaaagcagg gatgctcgat
     1921 tcgcaactgg aaatcctcga ggaactgggc gaaggcgaat acggggtcaa aatcgccaac
     1981 cccggcacgc ccgaagcggt cgaagccgat attctgaact gggttgcctc ggaaaacctg
     2041 ctttgaagca atatgccgtc tgaagcccga cacaggatgg gtttcagacg gcataaacat
     2101 cgggaacaga atggattaca ttgatttata gtggattaac aaaaaccagt ac
//
LOCUS       DAXHNE010000213         2142 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.213, whole genome shotgun sequence.
ACCESSION   DAXHNE010000213 DAXHNE010000000
VERSION     DAXHNE010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.213"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(422..895)
                     /locus_tag="ACFM4Z_002001"
     CDS             complement(422..895)
                     /locus_tag="ACFM4Z_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021972.1"
                     /translation="MKKQITEATMMLSIIAPAMANGLDNQAFEKYVFHTQADAPMQLA
                     ELSQKEMKETEGAFLPIVAGALMGGAISAWANHGISKIKTGQFASTRSTLAATAGGMI
                     GGGYSSAMLRGAGITTSVFARSAWKGGNAIPNAVIRANGAALNQSSSAAMGKHRR"
     gene            complement(984..1208)
                     /locus_tag="ACFM4Z_002002"
     CDS             complement(984..1208)
                     /locus_tag="ACFM4Z_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021973.1"
                     /translation="MMKNKYILIKAAIGITAISIFYLLTQESSGKTEEPSYFLMFMFL
                     NLLWFEENKTVMAAVTAMIAAHFIFVALSD"
     gene            complement(1478..1765)
                     /locus_tag="ACFM4Z_002003"
     CDS             complement(1478..1765)
                     /locus_tag="ACFM4Z_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021974.1"
                     /translation="MDDLIAAFLGYLSGKYREGKISRLGISLTAGSIFFVVFFIHELI
                     IFIVDGNKENLWSFITGIMKDLFLFSCSVCLLFYFLLFIAETIGKTINKIK"
BASE COUNT          700 a          445 c          324 g          673 t
ORIGIN      
        1 tgctacacct cgcataccat taatcagaat tcttcctatt ttttcgaatc cactatttct
       61 tccaaagatg cagcaaacgc aggttgagcc tagacccaat ctacgtttgc cgccgcatct
      121 gatccctatt gtttcttatc cttacatctt cctgccttgt caatcaaata aagacagaag
      181 actatacaaa aactgaccga catactgaaa atacctatcc ccttccatgc aatctccgca
      241 ccattgaccc aatagatcag actgagaaac aacaaagcca cagtataaat caacgtaaaa
      301 aatattgcgt aaagcacaat aaagggaact tttatctcac ggttgttttt tataatatat
      361 tcagcaactt gatttccgaa tatacctgat aaaaaatata gtattagatc atccatttcg
      421 tttatcttct atgttttccc attgcggcgc tagaagactg atttaatgct gcaccatttg
      481 cacgaataac tgcattagga atagcatttc cccctttcca agcagaacgg gcaaaaacac
      541 tagtagtaat ccctgcaccg cgcaacatcg cactgctata tccgcctcct atcataccac
      601 cagcggttgc agccaaggta cttcttgttg aagcaaattg ccctgtttta attttagaaa
      661 tgccgtgatt agcccaagca ctaattgccc ctcccatcaa agcacctgcc acaataggaa
      721 gaaacgcccc ctcagtctcc ttcatctcct tttgagaaag ctccgccaac tgcatcggcg
      781 catctgcctg cgtgtggaat acgtattttt caaatgcctg attgtccaat ccgtttgcca
      841 ttgcgggggc gattatagac agcatcatcg tggcttcagt gatttgtttt ttcataacaa
      901 attcttaggg aggagataat gttccatcaa aatcctcctg aaaggctgcc tgaaatttcc
      961 aagcagcctt tttgattgct taattaatcg gatagtgcga caaatataaa gtgggcggca
     1021 atcattgccg taacggcagc cataactgtt ttattttctt caaaccaaag caagttgaga
     1081 aacataaaca tcaagaaata tgatggttct tcagttttgc cgctgctttc ttgagttaga
     1141 agataaaata tagatatggc ggttatgccg attgctgctt ttattaaaat atatttgttt
     1201 ttcatcatct tgtatggttt ttcatgcaag tttcaacaat aaaattcgga ataagataga
     1261 gagatgttga tatttcagat aaatatacaa aacaatcaag aaactattgg cggtatgtat
     1321 aatcttgaat ttcttttttt gatgaaatta aaataatgaa atatatggta aacgttacaa
     1381 cggaagcctt agcctcccga ttgcagtatt tcaattccaa gataaatgcc gcatagaaga
     1441 taaaatactc aaatggtttt ttagcggctt aaatatctta tttaatctta ttgattgttt
     1501 tcccaatggt ttccgcaata aataataaga agtagaataa taaacaaaca gaacatgaaa
     1561 ataaaaataa atctttcatt atcccagtaa taaaactcca aagattttcc ttatttccat
     1621 caacaataaa aattatcagt tcatgaataa agaatactac aaagaaaatg cttcctgccg
     1681 ttagagatat tcctaacctg cttatttttc cttctctata tttacctgat aaatagccta
     1741 aaaaagctgc aatcaaatca tccattttaa aaatttctgt aaaaacaggt aatgtcttta
     1801 attcattacc tgttttgcca ctatatctat gatataagcg tctcttaccc ttcagcctct
     1861 ttgggaaatc aaagccctgc ttagaacgta tgttgcacac ccaatttcaa taggtaaatc
     1921 agattgcaaa tccagcaatt tgaatattgt cattgttccg tgcaaaaggg atttttattg
     1981 atgagttgtg tactgggttt cagcttggct ttttagataa tcttttcaca agaacataca
     2041 tcaatactgt ccaaaaaaaa agatttgata aatatattgc aacatatttt attccacctc
     2101 catccgaaac agaaaccaat atatttttaa ttaatacata gc
//
LOCUS       DAXHNE010000214         2134 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.214, whole genome shotgun sequence.
ACCESSION   DAXHNE010000214 DAXHNE010000000
VERSION     DAXHNE010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.214"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            425..1882
                     /locus_tag="ACFM4Z_002004"
     CDS             425..1882
                     /locus_tag="ACFM4Z_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6021975.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISSGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          497 a          624 c          493 g          520 t
ORIGIN      
        1 cggcgaagcc gcagacagta caaatagtac ggaaccgatt cactcggtgc ttgagcacct
       61 tagagaatcg ttctctttga gctaaggcga ggcaacgccg tactggtttt tgttaatcca
      121 ctataatgcg cccttcggcg tggcggatat ataaggaagt gattttccat ctaagtaaaa
      181 accgccctat cggataagcc cttaacagaa aaggctttac ccgcgccgta tcggaacaca
      241 tcctctaaaa tacaatccgt tgaattgaaa aaaatataaa aacatccgcc cgcgaaaaac
      301 ggcagcgcgt cgtttgacaa agaatgaaaa tatcggttaa aaaccgattt tcatacaaaa
      361 aacaccgccg ccgtccgcat ccgtttcaga cggtattgag agaaaatctt ttaggagaac
      421 ctttatgtcc cggcatcccg cccccaccgg agaaaaaaca ttcttcggcc accccttcca
      481 gctttccacc ctcttccata tcgaattgtg ggaacgtttt tcattttacg gaatgcaggg
      541 catcctgctg atttacctct actacaccgc cgacaaaggc ggcttgggca tagacaaaac
      601 cctcgccggc ggcattgtcg gcgcatacag cggcagcgtg tacctgtcca ccattttggg
      661 ggcgtggttt gccgaccgag tatggggtgc ggaaaaaacc ctcttcctct cgggcatcgt
      721 cgtgatgctc ggacacatcg tccttgccgc cgccccgggc ctgtacggcc ttttaatcgg
      781 gctgatattc atcgcattgg gcagcggcgg cgtgaaatct acggccagtt ctatggtggg
      841 cgcattatac gaacaggacg aaatgcgccc gctgcgcgat gcgggatttt ccattttcta
      901 catcgccatc aacatcggcg gcttcctagg cccgctgctg accggcctac tgcaggaaaa
      961 catcggtttc cattatggtt tcggcgcggc ggcggtcggt atggcattcg gcttgtggcg
     1021 ttattccctg ggacgtaaaa acctgcccca ccccaccgtc ccccatccgc tttcaaaagg
     1081 acagggcaaa actgcggccg ccgtcggcat cgccctcatc gccgcacttg caaccgccat
     1141 caaaaccggg cttgtcaacc tcgacaattt ctccggcatc ctattatcta ccgtcatcct
     1201 tgccgtcatc gcctatttcg cccgcctgct gaccaacccc cgcgtcagtt ccgacaacaa
     1261 acggcacatc atcgcctaca tcccgctttt cctgaccatc tgtatgtttt gggccgtctg
     1321 gtttcagatt tacaccgtgg caaccgtcta tttcgacgaa accgtcaacc gcaccatcgg
     1381 ttcgtttacc gtgcccgtcg cttggaaaga ttctatgcaa agcctgtggg tcatcctgtt
     1441 ttccggactg atggcggcaa tgtggacaaa aatggggcgc aaacagccca aaaccccgct
     1501 gaaattcgct atggcggtat ttgttaccgg cgcgtcgttt ttgggattcg tcccctttat
     1561 ttcctccggt acgccgatgc ctattgcggt tttcgcactg atcgtcctcg ccatcacgat
     1621 aggcgaactg atgatttccc cgattgcgct gtccatctcc accaaaatcg caccgccttt
     1681 attcaaaacc caaatggtcg cccttaattt ccttgccttt tcattaggct tcactttggg
     1741 cggcgtattg tttgaaaaag gctatcaggc gggcgacgaa atcggcttct atcggctgct
     1801 gttctacatc ggcgcagcca caggcttcct gctgctcctg ctcgtcccca aattgaacaa
     1861 aatgctcgaa ggcacagact aagtcccgcc ccgatgccgt ctgaaccctt cagacggcat
     1921 ttttccgcat aatgaaacca aaccgtttcc acccgacagg acaggctccc gcccaaccgg
     1981 aaggcagcct gccgattgtc atttgaataa cgcaagggaa agccgttgat ttccgtttgt
     2041 atggaaacag tttggtttca ttggaaaaag gcattttgtc cgactaaatt agtgctgcat
     2101 caacgaaata tatagtggat taacaaaaat cagg
//
LOCUS       DAXHNE010000215         2125 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.215, whole genome shotgun sequence.
ACCESSION   DAXHNE010000215 DAXHNE010000000
VERSION     DAXHNE010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.215"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            200..484
                     /locus_tag="ACFM4Z_002005"
     CDS             200..484
                     /locus_tag="ACFM4Z_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFC6021976.1"
                     /translation="MKLLAALIPLLMSVVGRILTALGLMAVTYSGVDRLVAHFQQAIT
                     HSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            485..1672
                     /locus_tag="ACFM4Z_002006"
                     /pseudo
     CDS             485..1672
                     /locus_tag="ACFM4Z_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin family protein"
     gene            1887..>2125
                     /locus_tag="ACFM4Z_002007"
     CDS             1887..>2125
                     /locus_tag="ACFM4Z_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFC6021977.1"
                     /translation="MNIIGLDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKAYIGMEATGIYYEKAADMLSSYYTVYVINP"
BASE COUNT          685 a          447 c          519 g          474 t
ORIGIN      
        1 taccccttgc cgccccgcca aaagaataca ctctgccgca agggcaggtg gtaaggcgcg
       61 cgctttttgc gccgttcccc ctgcccccgc ggcgtcgcaa gtgagactgg gggtgtgggg
      121 gctagtcccc gcaaaatctt tcagattaag aaacatttgt ttaatgaggc aaccgtgcct
      181 tttaagaaag ggatagcaaa tgaaattgtt ggccgcattg attccgctct tgatgagcgt
      241 cgtaggccgt atattgactg cattgggatt gatggccgtg acctattcgg gggtggatag
      301 attggtagcc cattttcaac aggcgataac ccatagcata acgggcgcac ctcaagcaat
      361 gttacagctt ttctatataa gcggcggtgg tactgttcta aacattcttt tcggcgcgat
      421 cgcctttatt ctgtcattca aacaaatgac aaaactagca acctcaatcg ggaagaaaaa
      481 ataaatggca gagatctgtt tgataaccgg cacgcccggt tcagggaaaa cattaaaaat
      541 ggtttccatg atggcaaacg atgaaatgtt taagccggat gaaaacggca tacgccgtaa
      601 agtatttacg aacatcaaag gcttgaagat accgcacacc tacatagaaa cggacgcgaa
      661 aaagctgccg aaatcgacag atgagcagct ttcggcgcat gatatgtacg aatggataaa
      721 gaagcccgaa aatatcgggt ctattgtcat tgtagatgaa gctcaagacg tatggccggc
      781 acgctcggca ggttcaaaaa tccctgaaaa tgtccaatgg ctgaatacgc acagacatca
      841 gggcattgat atatttgttt tgactcaagg tcctaagctt ctagatcaaa atcttagaac
      901 gcttgtacgg aaacattacc acatcgcttc aaacaagatg ggtatgcgta cgcttttaga
      961 atggaaaata tgcgcggacg atcccgtaaa aatggcatca agcgcattct ccagtatcta
     1021 tacactggat aaaaaagttt atgacttgta cgaatcagcg gaagttcata ccgtaaataa
     1081 ggtcaagcgg tcaaagtggt tttacactct gccagtaata gtattgctga ttcccgtgtt
     1141 tgtcggcctg tcctataaaa tgttgagcag ttacggaaaa aaacaggaag aacccgcagc
     1201 acaagaatcg gcggcaacag aacagcaggc agtacttccg gataaaacag aaggcgagcc
     1261 ggtaaataac ggcaacctta ccgcagatat gtttgttccg acattgtccg aaaaacccga
     1321 aagcaagccg atttataacg gtgtaaggca ggtaagaacc tttgaatata tagcaggctg
     1381 tatagaaggc ggaagaaccg gatgcgcctg ctattcgcat caagggacgg cattgaaaga
     1441 agtgacggag ttgatgtgca aggactatgt aaaaaacggc ttgccgttta acccatacaa
     1501 agaagaaagc caagggcagg aagttcagca aagcgcgcag caacattcgg acagggcgca
     1561 agttgccaca ttgggcggaa aaccgtagca gaacctaatg tacgataatt gggaagaacg
     1621 cgggaaaccg tttgaaggaa tcggcggggg cgtggtcgga tcggcaaact gaagaaaacg
     1681 gcaagagaga aaaaagaccc gtaaaccgtt tgaatataga cggtttacgg gtctttgttt
     1741 cgcgcaaagc aagggctaag gcagtcaggc agcaaatccc gcaatgtatt aaaacagacg
     1801 cgtagaaatg ccggctgcct ttatccatcc tcaaaattga atatcatcct agccgtatca
     1861 aggctgtata aataaggaaa ataccaatga atataatcgg gctggacatc tcaaaggaca
     1921 ccatagacgc aacattgcat aaaacaaacg gaagtatcca ttacattaaa tttaagaata
     1981 atgatgatgg attaaaacag tttagattgt ggataaaggg aaacagaatc agaaaagcct
     2041 atatcggcat ggaggcaaca ggcatctatt acgaaaaggc agcagatatg ctttcttcct
     2101 actataccgt ttacgttatc aatcc
//
LOCUS       DAXHNE010000216         2118 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.216, whole genome shotgun sequence.
ACCESSION   DAXHNE010000216 DAXHNE010000000
VERSION     DAXHNE010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.216"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(78..2093)
                     /locus_tag="ACFM4Z_002008"
     CDS             complement(78..2093)
                     /locus_tag="ACFM4Z_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family protein"
                     /protein_id="HFC6021978.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEVNAETRARFLNNDKARALS
                     DGILNQMQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEKPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWNERQLTQSGYPREVWLVERGKSFEESLPVYQIGEDGMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLRVSAEGEAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWNRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGINVQQFWTTSADGERIPYFHVGKNAAPDMPTLVYAYG
                     GFGIPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPEHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSAQSWLYSPDGGGHTGNGTQRESADELACVLLFLKEFLG"
BASE COUNT          442 a          638 c          582 g          456 t
ORIGIN      
        1 cattaaaaaa ggtcgtctga aaaagcagtt tcagacgacc ttttttcatt cgcggcggca
       61 gtgccgctcc cccgccctta gcccaaaaac tctttcaaaa acagcaagac gcaggcgagt
      121 tcgtcggcgg attcgcgttg ggtgccgttg ccggtatggc cgccgccgtc aggcgagtag
      181 agccaagatt gcgcggaggt ttcgcgcagt ttggcgtaga acttgagcgc gtgggcggga
      241 tggacgcgat cgtcggacag gctggtggta atgagcgcgg gcggataatc gatgccgtct
      301 gaaagattgt gatacggcga caattcgccc aaccggcgtt tgcagacttc gtatttttgc
      361 ggattgccgt attcgtctgt ccagcttgaa ccggcggaga gcagcggata acggatcatg
      421 tcggtcagcg gcacttcgca caccagcgcg ccgatgcttt gcggttcgcg cacgaaggcg
      481 gcggcagtaa tcagtccgcc gttgctgccg ccctgcaagc cgatgtgttc gggcgaactg
      541 ataccgcgtt cggacaaatc gcgcacgact gccaataaat catcaacgct tttatgtttg
      601 ctgattccct gcgccgcctg atgccagcgc gggccgaact cgccgccgcc gcggatgttc
      661 gccaatacaa aggcattgcc ctcttccagc caatatttgc caatgctgcc cagataatgc
      721 ggcaattcgg gaatgccgaa accgccgtag gcatagacca gcgtcggcat gtcgggcgcg
      781 gcgtttttgc cgacgtggaa ataaggaatg cgctcgccgt cagccgaagt cgtccaaaac
      841 tgctgcacgt taatgccgtc tgaatcaaac tgctgcggct ggcggcgcat gacggtcagt
      901 tccatcacgt tcaaatccag cgcaaacagc gtcagcggcg tggtgaaatc gctggcggca
      961 aggtaaacca cgtcgccgcc ccaaggttgg tcggtcattt ccaacgcgcc cgaaggcagg
     1021 cgcggcaatt cgacttcctg ccatttgccg tcggcaaacc gccatgcttt cagacggcct
     1081 tgtacgttct ccaacaggct cgccaccaca aaacgcttgg tcgtttccac gctttccaat
     1141 gcctgcgttt catcgggcgc aaacaaaagc tgcgccgccc cgagttcgcc ccgattcagc
     1201 ttcaccgcca ccagcgcgcc gctcggatag ctttggttcg cgcggttcca gtccttgcgc
     1261 agcgtcagca aaagatgccc cgccagatag ccgaccacgt cgcaatcgtt gggcaggttt
     1321 aacggtttcg cctcgccttc ggctgagacc cgcaaatagg ttttggtgta aaaaccgtcc
     1381 gacgcttcaa tcaaatcaat cggcgaaccc tgcggatcga gataacgcca cgcgttcacc
     1441 atcatgccgt cttcgccgat ttgatacaca ggcaggcttt cctcgaaact cttgccgcgt
     1501 tccaccagcc atacttcgcg cggatagccc gattgggtca actggcgttc gttccaagcc
     1561 ggacacaccc acacgctgtt ttcatcgcgc cacgacacat ggtttttgcc tgccggaaag
     1621 tgaaaaccgc cttctaccaa ttcccctgct tccaaatcca cttccagcgt gtacgccgta
     1681 tcaccgcccg acttgctcag tgttaacaac gcgcggttgg gcttttccac caagtgcgat
     1741 acgccgccca aatacacatc atcgccgagc aattcatcga aatccgccac tgaaaacagg
     1801 attttccact cgggatagcc ggaacggtaa gtcgccgccg tacacacgcg gtacacgccc
     1861 ttcggatatt ccgcgtcctg atggaaatgg tacatccgcg cgcggtgttc ctgacaaaac
     1921 ggaatctgcc gcgtgtcctg catttgattc aaaatgccgt ctgaaagcgc gcgcgccttg
     1981 tcgttgttta aaaaacgcgc gcgcgtttcg gcattcactt cagcagcgaa gttttgcgtt
     2041 tcggcggaat cgaggttttc aaaatggcgg taggggtcgg ggtaggattt catcggggtc
     2101 cttgagggtt gggcaggt
//
LOCUS       DAXHNE010000217         1949 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.217, whole genome shotgun sequence.
ACCESSION   DAXHNE010000217 DAXHNE010000000
VERSION     DAXHNE010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.217"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(237..560)
                     /locus_tag="ACFM4Z_002009"
     CDS             complement(237..560)
                     /locus_tag="ACFM4Z_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC6021979.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(548..787)
                     /locus_tag="ACFM4Z_002010"
     CDS             complement(548..787)
                     /locus_tag="ACFM4Z_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HFC6021980.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(910..1707)
                     /gene="truA"
                     /locus_tag="ACFM4Z_002011"
     CDS             complement(910..1707)
                     /gene="truA"
                     /locus_tag="ACFM4Z_002011"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFC6021981.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGEAVSTTVAGRTDTGVHATAQVVHFDTTAARPQQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRRAAAYLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSSGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          443 a          457 c          562 g          487 t
ORIGIN      
        1 ggtctggatt cccgcctgcg cgggaatgac ggctgcagat gcccgacggt ctttagagtg
       61 gattaacaaa aatcaggaca aggcgacgaa gccgcagaca gtacaaatag tacggaaccg
      121 attcacttgg tgcttcaaca ccttagagaa tcgttctctt tgagctaagg cgaggcaacg
      181 ccgtactggt ttttgttaat ccactataat gaacacaatc cattcagact attcaatcag
      241 gcaaacatct cctgcaatac tgcaaacagt ttttcagccg tactgttgtc taaatttcca
      301 agatgtttga ccaatccggc tttatccaca gccctaatct gttcgggcaa aagcaaaccg
      361 tctttatcct gaaagcggac attgacgcgg aacggggcag gacggcttcc gctcgtcatg
      421 ggaacgatca gcacagtctt gagatagttg tgtatttcag gaggagagac tacgacacaa
      481 ggacgtgtct ttttgatttc gcttcctacg gtcgggtcta aggagaccag atagattccg
      541 ccgcgtacta ccatatccat tctttatccg cttcgttttc aatttcggaa aaaaaatgct
      601 cctgctcggt ttcgacaagc attgcggcag cttctgccca tcccctgcga acggtaggac
      661 agcttaaaat aatattgccc ttttcaactg taacagccaa gctgtctact gcccctattt
      721 gacccaataa tgatttgggc agaatcacgc cttgcgagtt tcccattttg cgtatgttga
      781 gaatcatata cgtaccctat ttaccagttt atgtaataac aatgttatta ccttgatgag
      841 gtagtgtcaa tattgaaaaa gattgcctga cagtttgtcc gatttcaaaa tctccgcgac
      901 aagaatgttt taaagccatt cggggatttg ggggcggatg atgccgtatg cctcgggata
      961 gtcgacgccg gtcaggtaga gtccgtcggg catgaaggtc ggtggggctt tgaggcggct
     1021 gcgttcttga atcagtgcgg cgaagccttc gacgctgagt ctgccgctgc cgacataaac
     1081 gagcgcgccc atgatgttgc gtaccatatg gtgcaaaaag gcgttgccgt gcaaatcgag
     1141 gcggacgagt cctgagcttt gggtaaggtc ggcgcggtag atggttttga cgggggattt
     1201 tgcttggcat tcggcggcgc ggaagctgga gaagtcttgt tcgccgatca aataggcggc
     1261 agcccgacgc atcggttcga tgtcgagttt gaggtgtgtc cagcctgccc tgtttttgag
     1321 caggggggaa cggacggggg cggattcgag caggtagcgg tagtgccgtc cgtatgcgtc
     1381 gaagcgggcg tgaaattcgg gggcgacctg tcgggcgtgc aaaacggcaa tgccttcggg
     1441 caggtgggca tttacgccgc gcacccatgc ctgttgggga cgggcggcag ttgtgtcgaa
     1501 gtggacgact tgggcggtgg catgcacgcc ggtgtcggtc ctgccggcaa cggtggtgga
     1561 aaccgcttcc cctgctattt gggcgagcgc ggtttccaat gccgcctgaa cggtcggtac
     1621 gccgtcagcc tgtttctgcc agccgtaaaa gcggctgccg tcataggata gggttattgc
     1681 ccagcgttgt ttttgtgcgg tatccatcgg atttgggatt cggataaatg ttcagactgc
     1741 attgtatcgc agattttgca gggaaacggc aaacgcccag ggcgagcggc gttgtttggg
     1801 gagttggggg ggtggatgca gttgctacga atcgctatcc tgtgaattca ccctgtcagg
     1861 agtgcccgaa tcgtcattcc cgcgcagtcg ggaatctagg acgtaaaatc taaagaaacc
     1921 attttatagt ggattaacaa aaaccagta
//
LOCUS       DAXHNE010000218         1889 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.218, whole genome shotgun sequence.
ACCESSION   DAXHNE010000218 DAXHNE010000000
VERSION     DAXHNE010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.218"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            385..1830
                     /gene="zwf"
                     /locus_tag="ACFM4Z_002012"
     CDS             385..1830
                     /gene="zwf"
                     /locus_tag="ACFM4Z_002012"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFC6021982.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERFAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQACENLAAIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
BASE COUNT          505 a          427 c          500 g          457 t
ORIGIN      
        1 tatctgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc actatatttt
       61 aaaaggtatg ccgtctgaaa gtgggaagcg ggtgttatta tagtacaaaa tttgtaattt
      121 tgttacaaaa aaatcaggca ttgaaaagaa atttgattta aatcgcagaa tctttcgatt
      181 gttgtaacgg aataggggaa acctgcccga tttttggact aggtcaaata aattgtaaaa
      241 atgggaatag acaaaaagtg tagcggtgtg tagtattact actcattatg ccgcctgcct
      301 ttgtttccga tattgcgatg cagcgtgatg ggggaaacag gtttcagacg gcatcgaaaa
      361 attctttcct gacgagataa tacgatgagt acacaaacaa attttgattt ggtgttgttc
      421 ggtgcgaccg gcgatttggc gatgcgcaag ctgttgccct gtctgtacca ggcgcatgtt
      481 gccggtctgc ttcatcccga agggcgtatt ttgggggtca gccgcagcga gttggatacc
      541 gaaggttttc tggcgaaggt ggaaaccagt tccaaaatcc atgtgaaaga aaatttttcc
      601 gatgaggctt gggcatcgtt tgtcgaacgt ttcgcatatc tcaaagtcga tgttacgcag
      661 ccggacgatt ttgccgcttt gggtgatttg gtcaaagcgc gcaaagaaac cgacaatgtc
      721 gtcatctatc tttccaccgc gccgaaattc ttcgcgcaag cctgtgaaaa ccttgctgcg
      781 atcggtttga atgccgacaa tgtgcgcgta gttttggaaa aaccgttggg tacggatttg
      841 gcatcttccc aacaaatcaa taccgatgtt gcgcgttatt tcaaagaagg gcaaatctac
      901 cgtatcgacc attatttagg taaagaaagc ctgcaaaacc tgctggcctt gcgttttgcc
      961 aatgtgatgt tcgagccgct ttggaacaat aaatacatcg aaagcgtgca gctgaccatc
     1021 gccgagcagt tgggcgtgga agaacgcggc gagttttacg acattaccgg cgcgttgcgc
     1081 gatatggtgc aaaaccactt gatgcaaatg ctgtgcatga ctgcgatgga agcccccgcc
     1141 agcttggatg ccgacgcggt gcgcgatgaa aaagtcaaag tcatcaagtc attgaagccg
     1201 ctgaccgtcg aatctgtcaa tgaaaatgtc gtgcgcggac aatataccgc cgccaaaggc
     1261 atgaacggct atcttgaaga aatcaacgtt ccgcaagaca gctttaccga aacctacgtc
     1321 gccattaaag ccgaaatcga aaacgaacgc tggaagggcg ttcccttcta cctgcgtacc
     1381 ggcaagcgca tggcgggcaa agtggcggaa atcgttttga acttcaaaga tttgaacagc
     1441 catatttttg aaggcagtcg caccgcgccc aaccggctcg ttatcgagtt gcaaccatat
     1501 gaatccgtgc gcctctatac gcagatgaaa accccggggg caggaaataa ggtcgaaacc
     1561 gtgccgctgg caaccgattt gggcaaagca ttggaaagcc gccgcgcgga agcttacgag
     1621 cgcctgctgc tggatgtgat taacggcaaa ctcgctttgt ttaaccgccg cgacgaactt
     1681 gaagccgcgt gggaatatgt gatgccgatt ttggaaaact ggacaaataa caccacgccg
     1741 ccgcacggct atggcgcaca ctcgtggggg cctgaagccg cgcgcgaact attggcgcgc
     1801 gacggacaca agtggcacga agagcagtaa tacaataatg cgttcagacg gcatggggtt
     1861 tgaaatgccg tctgaacgta agtaaagca
//
LOCUS       DAXHNE010000219         1883 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.219, whole genome shotgun sequence.
ACCESSION   DAXHNE010000219 DAXHNE010000000
VERSION     DAXHNE010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.219"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(127..1437)
                     /locus_tag="ACFM4Z_002013"
     CDS             complement(127..1437)
                     /locus_tag="ACFM4Z_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFC6021983.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRHFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYSA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLEWLKSLDEEVLKAK"
     gene            1749..>1883
                     /locus_tag="ACFM4Z_002014"
     CDS             1749..>1883
                     /locus_tag="ACFM4Z_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HFC6021984.1"
                     /translation="MEWVSLFSDPQRDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     E"
BASE COUNT          450 a          481 c          494 g          458 t
ORIGIN      
        1 ttaatccact ataccacgtt gtctttattg gctttatcaa taaacaggat aagacctgaa
       61 aaaaagccga tacgcctttt tggtgtaccg gctttgccat actgttctgc ttcagacagc
      121 attgcttcat tttgccttta atacttcttc gtccagcgat ttcaaccatt ccagcttttc
      181 gccgattttg atttccaacc cgcgcgggac gggttggtag aagtccggtt cgtccaagcc
      241 gtcgggcata tagctttcgc cggcggagta ggcgttcggt tcgtcgtggg cgtagcggta
      301 ttcgcgtccg tagcccaatt ccttcatcag cttggtcggg gcgttgcgca ggtggacggg
      361 cacttcgtcg ctagcgtttt ctttgacgaa gtggcgcatt tggttgtatg ccttgtagcc
      421 cgcgttggat ttcgcggcgg cggcaagata caataccgct tgcgccaaag ccagttcgcc
      481 ttcgggcgag cctaagcgtt cgaaggtggc ggcggcatcg ttggcgattt ggaaggcgcg
      541 cgggtcggca agcccgatgt cttcccaagc gatacgcacg atgcggcggg cgaggtagcg
      601 cgggtcggtg ccgccgtcga gcatacggca gaaccaatac agcgcggcgt tcggatgcga
      661 accgcgcacg gatttgtgca gggcggagat ttggttgtag aaactctcgc cgcctttgtc
      721 gaaacggcgg atttgcgccc cgagactgtc ggcgagaaat tcggcggtta agtttttcag
      781 acgacgtgta tcggcggcgc gtaaaagttg ttccaacaaa ttcaacaatc tgcgcgcatc
      841 accgtcggcg gtattcacga gtaatttttg cgcatccgtt tcaatcgtaa actcttggta
      901 ttcaggcaaa gccaatacct tggcaatcag ctttttcagg tcgtctgaag acaagggttg
      961 caaaacatac acctgagcgc ggctcaacag cgcgggattg acttcaaacg acggattttc
     1021 cgtcgtcgca ccaataaagg ttagcaaacc gctttcgaca tgcggcaaaa acgcgtcctg
     1081 ctgcgccttg ttgaagcggt ggacttcatc gacaaacaaa atcgtcgcgc gtccctgctg
     1141 caaagcgatt tcggctttat cgattgcctc gcggatgtcc ttcacgccgg aaaatacggc
     1201 ggaaacaggc aaaaactggg cgttgaaact ctgcgccaaa atccgcgcca acgtcgtctt
     1261 gcccacgccc ggcggccccc acagcaacat agaatgcggc ttgccgcctt ctaccgccac
     1321 gcgcaaaggt ttaccttcgc cgatgaggtg ttcctgcccc accacgtcgt caagcgtatg
     1381 cggacgcaat cgttcggcaa gcggcgcgtc gggttctcgg gcaaacaaat cggtcataac
     1441 ggctccgtca acaggttttc aaacaatatg attatacggc agggaacggc ggcgtgccgc
     1501 atacggattc cgcccctccg tttgccttaa gccgatatta ggcgcatact ggaaaagacg
     1561 agagacttca cacaatatat ccggcacgga gaccgattcc gcatcggcat gacaataccc
     1621 aaatcagcgt ttcaattaaa cattaaggag actaaaatag aaaatttgct ttatctacca
     1681 ttgctttgtt gatttaatcg gcattatgtt ttgaggcgga agcccatgaa tatactaata
     1741 ttcaagagat ggaatgggtg tctttatttt ctgatccgca aagagacgat gatagtctta
     1801 taacccttaa agatgaaaaa atcactgtaa aaaactatat tgtgccttgg tggaaaaaag
     1861 gtgaaaactt tagaaaatta gaa
//
LOCUS       DAXHNE010000220         1857 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.220, whole genome shotgun sequence.
ACCESSION   DAXHNE010000220 DAXHNE010000000
VERSION     DAXHNE010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.220"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            114..833
                     /gene="pyrH"
                     /locus_tag="ACFM4Z_002015"
     CDS             114..833
                     /gene="pyrH"
                     /locus_tag="ACFM4Z_002015"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFC6021985.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDPSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVITGEDEGTLVHC"
     gene            1017..>1857
                     /gene="mafA"
                     /locus_tag="ACFM4Z_002016"
     CDS             1017..>1857
                     /gene="mafA"
                     /locus_tag="ACFM4Z_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC6021986.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSKSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFS"
BASE COUNT          525 a          477 c          418 g          437 t
ORIGIN      
        1 atttaaattt aatccactat aacagaacga cacgccatat cattgtaatt ccgaccgttt
       61 gaaattcaga cgacccaact ccgaccgacg acattcaaga aagcaaggta tccatgacac
      121 agcaaatcaa atacaaacgc gtattactga aactctccgg cgaatccctg atgggttccg
      181 atccgttcgg catcaatcac gataccatcg ttcaaactgt cggcgaaatt gccgaagtcg
      241 ttaaaatggg cgtgcaagtc ggtattgttg tcggcggcgg caatattttc cggggcgtat
      301 ccgcccaagc aggcagcatg gatcgcgcca ccgccgacta catgggcatg atggcgaccg
      361 tgatgaacgc gttggcactc aaagacgcat ttgaaacttt aggcatcaaa gcgcgcgtac
      421 aatccgcact gtctatgcag caaatcgctg aaacctacgc ccgccccaaa gccatccaat
      481 atttggaaga aggcaaagtc gtgatttttg ccgccggtac cggtaacccg ttcttcacga
      541 ccgacactgc cgccgcattg cgcggtgcgg aaatgaactg cgacgtgatg ctcaaagcca
      601 ccaacgtcga cggtgtgtac accgcagacc cgaaaaaaga cccgtccgcc acgcgctacg
      661 aaaccattac ttttgacgaa gccttgttga aaaacctcaa agtcatggac gcgaccgctt
      721 tcgccctctg ccgcgaacgc aagctcaata tcgtcgtctt cggcatcgcc aaagaaggct
      781 cgctcaaacg cgtcattacc ggcgaagacg agggaacgct ggttcactgc tgattgacca
      841 tagtgtcggc agatatagtc gcatatgggc ttcagacagc catttattat atggagatta
      901 tagtggatta aatttaaacc agtacggcgt tgcctcgcct tgccgtactg gtttaaattt
      961 aatccactat atttacaatt ttgatacaat ttgtttttca tcaaaggaga aaatctatgc
     1021 aagcacggct gctgatacct attctttttt cagtttttat tttatccgcc tgcgggacac
     1081 tgacaggtat tccatcgcat ggcggaggta aacgctttgc ggtcgaacaa gaacttgtgg
     1141 ccgcttctgc cagagctgcc gttaaagaca tggatttaca ggcattacac ggacgaaaag
     1201 ttgcattgta cattgcaact atgggcgacc aaggttcagg cagtttgaca gggggtcgct
     1261 actccattga tgcactgatt cgtggcgaat acataaacag ccctgccgtc cgtaccgatt
     1321 acacctatcc acgttacgaa accaccgctg aaacaacatc aggcggtttg acaggtttaa
     1381 ccacttcttt atctacactt aatgcccctg cactctcgcg cacccaatca gacggtagcg
     1441 gaagtaaaag cagtctgggc ttaaatattg gcgggatggg ggattatcga aatgaaacct
     1501 tgacgactaa cccgcgcgac actgcctttc tttcccactt ggtacagacc gtatttttcc
     1561 tgcgcggcat agacgttgtt tctcctgcca atgccgatac ggatgtgttt attaacatcg
     1621 acgtattcgg aacgatacgc aacagaaccg aaatgcacct atacaatgcc gaaacactga
     1681 aagcccaaac aaaactggaa tatttcgcag tagacagaac caataaaaaa ttgctcatca
     1741 aacccaaaac caatgcgttt gaagctgcct ataaagaaaa ttacgcattg tggatggggc
     1801 cgtataaagt aagcaaagga attaaaccga cggaaggatt aatggtcgat ttctccg
//
LOCUS       DAXHNE010000221         1811 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.221, whole genome shotgun sequence.
ACCESSION   DAXHNE010000221 DAXHNE010000000
VERSION     DAXHNE010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.221"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(446..1441)
                     /gene="porB"
                     /locus_tag="ACFM4Z_002017"
     CDS             complement(446..1441)
                     /gene="porB"
                     /locus_tag="ACFM4Z_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HFC6021987.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVEHNGGQV
                     VSVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGKYNSESYHAGFNYKNGGFFVQYGGAYKRHVRVDENVNIEKYQIHRLVS
                     GYDNDALHASVAVQQQDAKLVKDNYSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSF
                     DDADLSNDYDQVVVGAEYDFSKRTSALVSAGWLQEGKGENKFVSTAGGVGLRHKF"
BASE COUNT          476 a          411 c          445 g          479 t
ORIGIN      
        1 tcggaaatgg ctgaaactca aaaaactaga ttcccacttt cgtgggaatg acggcagagc
       61 ggattctgtt gctcccgata aatgccgcaa cctcaaatcc cgtcattccc gcgcaggcgg
      121 gaatctaggt ctgtcggtgc ggaaacttat cggataaaac ggtttcttga gattttacgt
      181 tctggattcc cactttcgtg ggaatgacgg gattaaagtt tcaaaattta ttctaaatag
      241 ctgaaactca acgcactaga ttcccgcctg cgcgggaatg acggcatatt ttgacattga
      301 ataaaaaaga ctaaaaacag aaaacagcca aacagaaaaa agccaaacag aaaaaagcca
      361 aacagaaaaa agccaaacag aaaaaagcca aacagaaaaa agcctgtctg gcgacaggct
      421 ttttgttgat accaatcttt gcagattaga atttgtggcg cagaccgaca ccgccggcag
      481 tcgatacgaa tttgttttcg cctttacctt cttgcaacca accggcagaa accaaggcag
      541 aagtgcgttt ggagaagtcg tattccgcac cgacaaccac ttggtcgtaa tcgttgctta
      601 agtctgcatc atcaaacgag cctttgaagc cgtgggcgta agaaacgcgg ggcgttacgt
      661 tgccgaagcg gtatgccaag gtagcggcaa cttcggtttg agagttgtgc gaataattgt
      721 ctttaaccaa tttggcatct tgttgctgta cggctacgga agcgtgcagg gcatcattgt
      781 cgtaaccgct gaccaaacgg tgaatctggt atttctcaat attcacgttc tcatccaccc
      841 gcacatgtct tttataggca ccgccatatt gcacgaagaa gccgccgttt ttgtagttga
      901 agccggcgtg gtaagattcg ctgttatatt tgcctgcatt gtcgttaagc gcgtattgta
      961 cgctgccgct gaggccggca aattcgggag aatcgtagcg tacggaaatg aggcgtgctt
     1021 cgggttcggc aattttgttt acacccaaat agtcgctttt gctatcccaa ggattgatgt
     1081 cgccggtgtc tttcaggacg ctgttcaaac ggccgacgcg caatttaccg aagccgcctt
     1141 tcaaaccgat gaaggattgg cggttgcccc aaccggagtc agtaccggcg atagatgcct
     1201 tttgctcaac ctgccaaatg gctttcagac cgttaccgag gtcttcttgg cctttgaagc
     1261 cgatttttga acccaaatca acgatgccgg taccggtttc aacgctaacc acctgacctc
     1321 cattgtgctc tacagagcgg gaagtttcta cgccggcttt gatggtgccg tacagggtaa
     1381 cgtcagccat tgctgcaaca ggaagggctg ccaaagtcag ggcaatcagg gattttttca
     1441 ttgctgtatt ccttttttgg ttaagaaatt taagcaggcc gggctttcca agccgcttag
     1501 ctttgcattt accgccgacg ttttcggcga ttccatgtct gaaactatag atgttcctgt
     1561 ctgaaaaaac aaacttctgc ctgtttttgt gtggaattcg atgtgtattt tgaagggcgg
     1621 atagagatat tgtgggtatt ttttgtttta taacatatgg ttatttaaat tttttaagat
     1681 ttgcattttt acaacactta ctcgggaggg tattggaggg cattgcaaac cgggggttat
     1741 aaagacggca aaaaacgcac acgcggtttc tttcggaacg ggcggatgca aaacccgacc
     1801 ctgcggcagg a
//
LOCUS       DAXHNE010000222         1737 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.222, whole genome shotgun sequence.
ACCESSION   DAXHNE010000222 DAXHNE010000000
VERSION     DAXHNE010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.222"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..78
                     /locus_tag="ACFM4Z_002018"
     CDS             <1..78
                     /locus_tag="ACFM4Z_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HFC6021988.1"
                     /translation="EKAWNLYEKNKTVQQTQPFQLHRCG"
     gene            156..485
                     /locus_tag="ACFM4Z_002019"
     CDS             156..485
                     /locus_tag="ACFM4Z_002019"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6021989.1"
                     /translation="MGSLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGARGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            514..720
                     /locus_tag="ACFM4Z_002020"
                     /pseudo
     CDS             514..720
                     /locus_tag="ACFM4Z_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249814.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            923..1606
                     /locus_tag="ACFM4Z_002021"
     CDS             923..1606
                     /locus_tag="ACFM4Z_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC6021990.1"
                     /translation="MTGFNDAAGVASVSTDIKGKYVQSVTVANGVITAQMASSGVNNE
                     IKGKKLSLWAKRQDGSVKWFCGQPVTRAAKAKDDVTADTTTNTKIDTKHLPSTCRDAA
                     SAVCTKTPEYYPNHGEWPSDNSAAGVASSSTIKGKYVEKVEVKNGVITAEMKSSGVNK
                     EIQGKKLSLWAKRQDGSVKWFCGQPVTRNAANAKAGTDEVTAATPDTDKINTKHLPST
                     APTRKSTPN"
BASE COUNT          515 a          498 c          417 g          307 t
ORIGIN      
        1 gaaaaagcat ggaaccttta cgaaaaaaat aaaacagtcc aacaaacaca gccgtttcaa
       61 ctgcaccggt gcggctgacg tttgagcggc atttgacttc gccggacggt atgttaccct
      121 tgccgttttg accaacaagc aacgaaagga caaatatggg cagcctgatt attgaagatt
      181 tgcaggaagg cttcggaaaa gaagcagtta aaggcaaaga gattaccgtg cattacacag
      241 gttggctgga aaacggaacc aaattcgact ccagcctcga ccgccgccag ccgctgacca
      301 tcacgctcgg cgtcggacaa gtcatcaaag gctgggacga aggcttcggc ggcatgaagg
      361 aaggcggcaa acgcaagctg accatccctt cggaaatggg ctacggcgca cgcggcgcgg
      421 gcggcgtgat tcctccgcac gccactttga tatttgaagt cgagctgctg aaagtgtacg
      481 aataaagctg cccgggcaat accgtctgac ataatggctt caagcaacgt aaacaacgaa
      541 atcaaagaca aaaaactctc cctgtgggcc aagcgtcaag acggttcggt aaaatggttc
      601 tgcggacagc cggttacgcg caacgacacc gccaaagacg acaccgtcgc cgccgacacc
      661 gacaccgcca agaagatcga caccaagcac ctgccgtcaa cctgccgcga tgattcatct
      721 accggttgca tagaaacacc acgcgccgac ttcaaacact tccaaaaaat cagccgttac
      781 cgagtattgc ccgaatcacg gcacatggcc gaaaaacttc gtcattcccg cgcaggcggg
      841 aatctaggtc tgtcggcacg gaaacttatc gggtaaaacg gtttcttgag attttgcgtt
      901 ctagattccc actttcgtgg gaatgacggg atttaatgat gccgccggcg tggcatccgt
      961 ctccaccgac atcaaaggca aatatgttca aagcgttaca gtcgcaaacg gcgtcattac
     1021 cgcccaaatg gcttcaagcg gcgtaaacaa cgaaatcaaa ggcaaaaaac tctccctgtg
     1081 ggccaagcgt caagacggtt cggtaaaatg gttctgcgga cagccggtta cgcgcgccgc
     1141 caaagccaaa gacgacgtta ccgccgacac caccaccaac accaagatcg acaccaagca
     1201 cctgccgtca acctgccgcg acgcagcatc tgccgtttgc acaaaaacac ccgagtatta
     1261 cccgaatcac ggcgaatggc cgtccgacaa tagtgccgcc ggcgtggcat cttcttcaac
     1321 aatcaaaggc aaatatgttg agaaagttga agtcaaaaac ggcgtcatta ccgccgaaat
     1381 gaaatcaagc ggcgtaaaca aagaaatcca aggcaaaaaa ctctccctgt gggccaagcg
     1441 tcaagacggt tcggtaaaat ggttctgcgg acagccggtt acgcgcaacg ccgccaacgc
     1501 caaagccggc accgacgaag tcaccgccgc cacacccgac accgacaaaa tcaacaccaa
     1561 gcacctgccg tcaacagcac cgacaagaaa atcgacacca aactagagaa acctttttac
     1621 ccgataagtt tccgtaccga caaggctgga ttcccgcctg cgcgggaatg acggcggagc
     1681 ggtttctgtt ttttccgata aattcctaaa acttaaaatt tcatcattcc cacaaga
//
LOCUS       DAXHNE010000223         1722 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.223, whole genome shotgun sequence.
ACCESSION   DAXHNE010000223 DAXHNE010000000
VERSION     DAXHNE010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.223"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(55..834)
                     /locus_tag="ACFM4Z_002022"
     CDS             complement(55..834)
                     /locus_tag="ACFM4Z_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFC6021991.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
     gene            1337..1722
                     /locus_tag="ACFM4Z_002023"
                     /pseudo
     CDS             1337..1722
                     /locus_tag="ACFM4Z_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311098.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
BASE COUNT          434 a          519 c          351 g          418 t
ORIGIN      
        1 aatttagttc actataccga tacatccgaa tatgccagac cgtctaaacg gtattcatac
       61 ctcccaatcc tgcacgcgct tcaaatcgtt ttgcgccaaa gtatccgcgt ggcggttacg
      121 gctctgatat tccctgtctt tcatgatgct gcttgccaca taattcaaat gtgcctttgc
      181 cgcctccgaa gcctcgcccg gccgacggtt cgatatcgcc tcatacagca cgcggtgctg
      241 cgccatcagt ttcggacgcg gatcttcttc ctgattcaga taaataaggc tgctgcgcgt
      301 ctgccggtac agcattttca acaaaccgcc cgacaaatgg ctgaacaaca aattgtgcgc
      361 cgcatccgca atcgtctgat ggaagctgac atcggcttcg ctctgatgtt ctagattgcc
      421 gctttcgcac gcctcctcaa acttttcaag ccaaaaccca atccgcttca aatcggcatc
      481 cgtccggcgt tctgccgcca atgccgccat acagccctcg atgtggcagc tgaaatcaaa
      541 aacatcctgt tcccaattgg aatgcttgcc caaaagctcc tgccaacttt gcaaaaaatc
      601 ctgctgcggc ttgaccgaaa cataataacc gtctccctgc ctcgcttcca aaacctgacg
      661 ggcgaccaaa acattcaatg ccgacctgac cgacgggcgc gaaacgccga actcttccgc
      721 caaaacgcgt tcgggcggaa tcttgccccc ttccgcgtaa accccttccg caatgcgctc
      781 ctccaatacc gacaatacct gatcgctgat tttctgaggc cttaccagtt tcatcactcc
      841 tcctttataa agattccctg cagaaccctt ccgaaatata gtggattaac aaaaatcagg
      901 acaaggtgac gaagccgcag acagtacaaa tagtacagaa ccgattcact tggtgcttca
      961 gcaccttaaa gaatcgttct ctttgagcta aggcgaggca acgccgtact ggtttttgtt
     1021 catccactat acatcaaaca tcaaattgga ctgaccaatc agggcggatt ctaatgacac
     1081 gccgttcccg ccgtcaacgg catttacctc gcaccgcccc cgaaacacaa gaaaaaacta
     1141 caccaaacta caatttttgt tcatgcaaat atttgttttg acaggattta aacgaaagct
     1201 ccgattcaaa tctgccgaac cgcccaaaaa tatattgacc taaatattaa agtttcgtaa
     1261 agtaatgcaa cgttgcttta attggtttga ccactattgc cgacgattag aaaaatattt
     1321 tcggagatgt tcaattatgg aaacttgggt tcaaaactac acggcaatcg gcggcagcct
     1381 gtatctgact gccgccgccg cactcttacc catcgtcttt ttctttgccg cgctgaccgt
     1441 cctgaagctg aaaggctatc aggcggggct ttatacgctg ctgattgcgc ttgccgttgc
     1501 cgtattcggc ttcgggatgc cggcggtatg gcggtttctt ccctgccgca gcagccgcac
     1561 tgacccaacg cccctacgcc acgctggatt tgaccgcgca ttacaaaatc ggcaaatcca
     1621 cccgcatcgg tttggacttt gaaaacgtgt tcaacaaacg ctaccgccct atgcccgaca
     1681 ttcacgttta cggcacgccg cgcagcctga ccgcaaccgt ca
//
LOCUS       DAXHNE010000224         1719 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.224, whole genome shotgun sequence.
ACCESSION   DAXHNE010000224 DAXHNE010000000
VERSION     DAXHNE010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.224"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            342..1646
                     /locus_tag="ACFM4Z_002024"
     CDS             342..1646
                     /locus_tag="ACFM4Z_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFC6021992.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDATAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGVVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          352 a          431 c          509 g          427 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggatcggat gcgatatttc catattcaaa caagggattg gcggcacatc ggcaaaatcc
      241 ccgcgccgcc ccggtccggc agggcttgcg tccctcccgg acaagccccg accccgcctt
      301 tccgaaagac gggctcaacc attaaggaaa ctttagtcaa aatgaaaaaa cacatatggg
      361 cggcatcttt gctgccggca tccctatcgg cagaaccttt aaactggtgg aagccttatt
      421 ccgccgtcaa ttcgggcgat accgcctggg tgatgactgc ggctgccttg gtactgttga
      481 tgacgcttcc cgggctggct ttattctacg gcggtatggt gcggaaaaaa aacctgctct
      541 cgacgatgat gcacagcttt tccatcgcga cattggtggg catcctttgg gtcgccgtcg
      601 gctattcttt agcgttcacg ccgggaaatg cctttatcgg cggtttgggg cgcgtatttt
      661 taagcgggat gcagatagac gctaccgcac agatgctgac cgtgtcgccc aatgcgccga
      721 ctgttcccga accggtattt atgttttttc agatgacgtt tgccattatt tcgaccgcca
      781 ttattaccgg cgcgtttgcc gaacggatga aatattcggc aatgatgctg ttttcgggca
      841 tatggttttt attggtttat gtgccgggcg cgcattgggt gtggggcggc ggctttatga
      901 gcaagggcgg cgtattggat tatgccggcg gtacggtggt gcacatcaat gccggtatcg
      961 cgggactcgt cgccgccttg gttttgggca ggcgcatagg ctacgggcgc gaggcgatgc
     1021 ctccgcacaa tatggcgatg acactgatcg gcgcggcaat gttgtggttc ggctggttcg
     1081 gctttaacgc cggatcggcg cttgcggcag acgcggcggc gggtatggcg atggcggtaa
     1141 cgcaggtgtc ggccgtattc ggcgcggcag gctggcttgc ctgcgaaaaa atagcgggac
     1201 acaaaccttc cgctttgggg ctggcttccg gcgcggtttc cggtctggtc ggcatcaccc
     1261 ctgccgccgg ctttaccggc ccgtcgggcg cggccgccat cggtatattg actgccgccg
     1321 cgtgctttgt gtccgtcacc gtcgtcaaac acaaattgcg ttacgatgat tctttggacg
     1381 ctttcggcat acacggattc ggcgggctgg tgggcggaat attgaccggc atctttttcg
     1441 acaaccgcat tttcggcggg gatgcggcag tttggcagca gttgtggata caggtaaaag
     1501 acggggtcgt tatggcggca tacagcgggc taatgagttg ggcgattttg aaggtcgtgg
     1561 ggaaaatctg cggcggcctg cgcgtcggca aggatgtcga acgcgaaggt ttggatctga
     1621 atatccacgg cgaacgcgtg gaataagggc ggctatgccg tctgaagcct gaaaatcggg
     1681 tttcagacgg catttttcac gtttgccgcc gatggataa
//
LOCUS       DAXHNE010000225         1655 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.225, whole genome shotgun sequence.
ACCESSION   DAXHNE010000225 DAXHNE010000000
VERSION     DAXHNE010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.225"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            295..1461
                     /locus_tag="ACFM4Z_002025"
     CDS             295..1461
                     /locus_tag="ACFM4Z_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFC6021993.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNYQLQLTEQP
                     SRTQGQTSNQVKVTKAKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGG
                     GATRWGNRESFVGLAGEFGTLRAGRVANQFDDASKAIDPWDSNNVVASQLGIFKRHDD
                     MPVSVRYDSPDFSGFSGSVQFVPAQNSKSAYKPAYVDEKKMVHAAVVGKPGSDVYYAG
                     LNYKNGGFAGNYAFKYAKHANVGRDAFNLFLLGRIGDDDEAKGTDPLKNHQVHRLTGG
                     YEEGGLNLALAAQLDLSENGDKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIERGK
                     KGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          423 a          380 c          426 g          426 t
ORIGIN      
        1 gccgcgtgtg tttttttatg gcgttttaaa aagccgggac tgcttctagg cagtagcgca
       61 tcggcccgca cgaggtctgc gcttgaattg tgttgtagaa acacaacgtt ttttgaaaaa
      121 ataagctatt gttttatatc aaaatataat cattttttaa aataaaggtt gcggcattta
      181 tcagatattt gttctgaaaa atggtttttt gcgggggggg ggtataattg aagacgtatc
      241 gggtgtttgc ccgatgtttt taggttttta tcaaatttac aaaaggaagc cgatatgcga
      301 aaaaaactta ccgccctcgt attgtccgca ctgccgcttg cggccgttgc cgatgtcagc
      361 ctgtacggcg aaatcaaagc cggcgtggaa ggcaggaact accagctgca attgactgaa
      421 caaccctcaa gaactcaagg tcaaacgagc aatcaggtaa aagttactaa ggccaaaagc
      481 cgcatcagga cgaaaatcag tgatttcggc tcgtttatcg gctttaaggg gagcgaggat
      541 ttgggcgaag gtctgaaggc tgtttggcag cttgagcaag acgtatccgt tgccggcggc
      601 ggcgcgaccc gttggggcaa cagggaatcc tttgtcggct tggcaggcga attcggcacg
      661 ctgcgcgccg gccgcgttgc gaatcagttt gacgatgcca gcaaagccat tgatccttgg
      721 gacagcaata atgttgtggc ttcgcaattg ggtattttca aacgccacga cgatatgccg
      781 gtttccgtac gctacgactc tccggacttt tccggtttca gcggcagcgt ccaattcgtt
      841 ccggctcaaa acagcaagtc cgcctataag ccggcttatg tggatgagaa gaaaatggtt
      901 catgcggctg ttgtcggcaa gcccggatcg gatgtgtatt atgccggtct gaattacaaa
      961 aatggcggtt ttgccgggaa ctatgccttt aaatatgcga aacacgccaa tgtgggccgt
     1021 gatgctttta atttgttctt gcttgggcgc atcggcgatg atgatgaagc caaaggtacc
     1081 gatcccttga aaaaccatca ggtacaccgc ctgacgggcg gctatgagga aggcggcttg
     1141 aatctcgcct tggcggctca gttggatttg tctgaaaatg gcgacaaaac caaaaacagt
     1201 acgaccgaaa ttgccgccac tgcttcctac cgcttcggta atgcagttcc acgcatcagc
     1261 tatgcccatg gtttcgactt tatcgaacgc ggtaaaaaag gcgaaaatac cagctacgat
     1321 caaatcatcg ccggcgttga ttatgatttt tccaaacgca cttccgccat cgtgtctggc
     1381 gcttggctga aacgcaatac cggcatcggc aactacactc aaattaatgc cgcctccgtc
     1441 ggtttgcgcc acaaattcta aatatcgggg cggtgaagcg gatagctttg tttttgacgg
     1501 ctcgccttca ttctttgatt gcaatctgac tgccaatctg cttcagcccc aaacaaaaat
     1561 ccggatacgg aagaaaaacg gcaataaaga cagcaaatac cgtctgaaag attttcagac
     1621 ggtatttcgc atttttggct tggtttgcac atata
//
LOCUS       DAXHNE010000226         1635 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.226, whole genome shotgun sequence.
ACCESSION   DAXHNE010000226 DAXHNE010000000
VERSION     DAXHNE010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.226"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..380)
                     /locus_tag="ACFM4Z_002026"
     CDS             complement(<1..380)
                     /locus_tag="ACFM4Z_002026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021994.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMY"
     gene            complement(438..803)
                     /locus_tag="ACFM4Z_002027"
     CDS             complement(438..803)
                     /locus_tag="ACFM4Z_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021995.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(785..1330)
                     /locus_tag="ACFM4Z_002028"
     CDS             complement(785..1330)
                     /locus_tag="ACFM4Z_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021996.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETERAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIPG
                     GVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQ
                     GIGRHRPWESKSTDRSWKNRF"
BASE COUNT          509 a          360 c          253 g          513 t
ORIGIN      
        1 ctatacatct caatacgata aatatttctt aagtcaaaat gcaagcctga ccgtacctta
       61 actgtcaaaa ttttattatt ttttattgat tttaaaacaa tttctgcaaa attctcttcg
      121 ctttctctct tttttagaag cacataagaa aaaataaaac ttccccgatt aaattcataa
      181 atatgtttca accatttgcc tcctctttct gtaagacaag attcagtttc attcttcctt
      241 attgtataaa tatttccttc acaaaatctg aaataaatcc ataaatccat cttatccata
      301 attaaagaaa aagtttcacc tcgagatttt gtcaacaatt cgcaaggttg cgatgttgca
      361 atcaaatagc cgaaagacat tttttacctc atacatggtc gaaatcagtt tctgttagtt
      421 cagaatccat tttttcgtca acaactgaat ccgcattttt gaattaacgt tttcatcagc
      481 tgccgtttat ctaaaccggc aggttcagtt tcaaaataag ccttatatga agactgtaag
      541 catttcagaa aaagatcatc agaagacata tctgccgaat caaatacaac tgttttgatt
      601 ttggtactta cccaaaaccc tttttgctct ttttctacta tacggaaatt cagaatattt
      661 ccaaccgaat caaaagcacg gtaaacatca tccatcaaat cctgcggctc tattttcttt
      721 tccaattccg acaatccttg aaatatatcc aaagacacat cttcaaatag aaaaaaagga
      781 ggagttagaa gcggtttttc catgatctgt ccgtagattt tgattcccaa gggcgatgac
      841 gaccaattcc ctgtccaggc aaagtcttgc ccgtattatc cgtaactcga cgatgataat
      901 ggggaaattt tccaatagga tgacctgttc tattaccgaa aggggctatc cgcatattat
      961 tgccgatttt aatctcacgt ccatatttag caaaggaaac aacctttcct gcggcgccta
     1021 caccaccagg aattgcgcct aatccgccag caatagcaac atctctaaca gaagctggtc
     1081 tgcctgtcgt tgcataacta aaaccatgct gtgtccacat accaatggca gcaccaccca
     1141 agatagccaa tggaagaaac gccctctcag tctccttcat ctccttttga gaaagttccg
     1201 ccaactgcat cggcgcatct gcccgcgtgt ggaacacttg gtcttcaaat gcctgattgt
     1261 ccaagccgtt tgccattgcg ggggcaatca tagacagcat cattacggct gcggtgattt
     1321 gttttttcat aataactcct ttggattaca aggttggaaa atcaaagccc tgcttagaac
     1381 gtatgttgca cacccaattt caataggtaa atcagattgc aaatccagca atttgaatat
     1441 tgtcattgtt ccgtgcaaaa gggattttta ttgatgagtt gtgtactggg tttcagcttg
     1501 gctttttaga taatcttttc acaagaacat acatcaatac tgtcccaaaa aaaagatttg
     1561 ataaatatat tgcaagatat tttgttccat cttcatccga aacagaaacc aatatatttt
     1621 taattaatac atagc
//
LOCUS       DAXHNE010000227         1631 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.227, whole genome shotgun sequence.
ACCESSION   DAXHNE010000227 DAXHNE010000000
VERSION     DAXHNE010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.227"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..788)
                     /locus_tag="ACFM4Z_002029"
     CDS             complement(<1..788)
                     /locus_tag="ACFM4Z_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6021997.1"
                     /translation="MYALSEKYNDNGFKAYKVLGEGGGIHTEYNYKFDKSLNLNVLES
                     STGARSLEKVPVKFTASVSRAAVLAGVGKLARLGAKFSTRAVPYVGTALLAHDVYETF
                     KEDIQAQGYQYDPETDKFVKGYEYSNCLWYEDERRINRTYGCYGVDSSIMRLMSDYSR
                     FPEVKELMESQMYRLARPFWNWHKEELNKLSSLDWNNFVLNSCTFSWNGGDCSVNKGD
                     DFRNGANFSLIRNPKYKEEMDAKKLEEILSLKVDANPDKYIKATG"
     gene            complement(829..1119)
                     /locus_tag="ACFM4Z_002030"
     CDS             complement(829..1119)
                     /locus_tag="ACFM4Z_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021998.1"
                     /translation="MNKNERDFFYISNSDLDKLSEFYPDRPLSYVFYCYLKETGLLEN
                     FSMDKCHNFFNRINFNESCFEIKFKDDSFFIIGNGKIDVSDSNNFFSVSFEC"
     gene            complement(1235..1537)
                     /locus_tag="ACFM4Z_002031"
     CDS             complement(1235..1537)
                     /locus_tag="ACFM4Z_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6021999.1"
                     /translation="MGFRVGMNCFDTRLQADDYLLSSLPPTVTQDGKIIRPERVGEKW
                     VLNGKPVTLSYPECSNFEQIKQGSYVGSTVLILFVVIFGFRLLINFLKDMGKVGID"
BASE COUNT          517 a          353 c          266 g          495 t
ORIGIN      
        1 ccggttgcct ttatgtattt gtcgggattg gcatcgactt tcaacgataa aatctcttcc
       61 agctttttgg catccatttc ttctttgtat ttcggattgc ggataaggga aaaattagcc
      121 ccatttctga aatcatcacc tttattgacc gaacaatctc cgccgttcca actaaatgtg
      181 caactattta aaacaaaatt attccaatcc aaagaactta atttattcag ttcttcttta
      241 tgccaattcc aaaacggacg tgccagccta tacatttggc tttccatcaa ttctttgact
      301 tcggggaatc tgctgtaatc ggacataagg cgcataatcg aactgtcaac gccgtagcag
      361 ccataggttc tattaatacg tctttcgtct tcgtaccaaa ggcaattact atattcgtag
      421 ccttttacaa atttgtcggt ttcgggatcg tattggtagc cttgtgcctg tatgtcttct
      481 ttgaaagttt cgtatacgtc gtgggctaaa agggctgttc cgacataggg aaccgccctt
      541 gtgctgaatt tcgcgcctaa gcgggcaagt ttgccgactc ctgccaagac ggcggcgcgg
      601 gaaactgatg cggtaaattt aacggggact ttttcaagag agcgtgcacc tgttgaactt
      661 tctaatacat tcaaattcaa acttttatca aatttgtaat tgtattctgt atggattccc
      721 cctccttcac ctaaaacttt gtatgccttg aatccattat cgttatattt ttccgaaagt
      781 gcatacatca atttcccatt tttaatttct aaatctgccg aaaaagattt agcactcaaa
      841 agaaacagaa aagaaattat tagaatccga aacatcaatt tttccattgc caataatgaa
      901 aaatgaatca tccttaaatt taatttcaaa gcaagattca ttaaaattaa ttctattaaa
      961 aaaattatga catttatcca ttgagaaatt ttccaataaa ccagtttctt tcaaataaca
     1021 ataaaacaca taagaaagag gcctatcagg ataaaattct gacaatttat ctaaatcaga
     1081 atttgatata tagaaaaagt cacgttcatt tttattcatg gtttcaagcc tcaaatgtct
     1141 aattaaaagc ctgattttaa aacacgtcat ataaatatca aagcaacaga caaagccaag
     1201 aagaaaccaa gaagaaacca aaaatcgata accatcaatc aatcccaacc ttgcccatgt
     1261 cttttaagaa attaatcaga agcctgaaac cgaaaatgac tacgaacaga attagaaccg
     1321 tcgaaccgac ataagaacct tgctttatct gctcaaaatt ggaacattcc ggataagaca
     1381 acgtaaccgg ctttccgttc aagacccatt tttcgcccac cctttccggc ctgatgattt
     1441 ttccgtcctg ggtaacagta gggggaaggg acgacaataa atagtcgtct gcctgcaatc
     1501 ttgtatcaaa acaattcata ccgacacgaa agcccattta cgcgccccct ctttcttcac
     1561 tgccgttatt tgacagattt gatcatgctc caagccattt tgaagccttg gattgcaaga
     1621 attacggtga t
//
LOCUS       DAXHNE010000228         1608 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.228, whole genome shotgun sequence.
ACCESSION   DAXHNE010000228 DAXHNE010000000
VERSION     DAXHNE010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.228"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            75..1448
                     /locus_tag="ACFM4Z_002032"
     CDS             75..1448
                     /locus_tag="ACFM4Z_002032"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC6022000.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGREIVVATVGRLLDHVKQKNIHLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGNLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKPEMREPRQRNRYESAKAQREKNTRPENAANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
BASE COUNT          420 a          527 c          386 g          275 t
ORIGIN      
        1 taatccacta tatttccccg tcctatcggt ttcccgtttc agacgacata aggtctgaaa
       61 gaaagactac aattatgagt aatccatttt cctctttagg tttgggtacg gaactcgttt
      121 ccgcactgac cgcgcaaggt tacgaaaacc cgacgcccat ccaagccgcc gccattccca
      181 aagcactcgc cggtcatgat ttgctagccg ccgcgcaaac cggcacaggc aaaaccgccg
      241 cctttatgct gcccagtctg gaacgcctca aacgttacgc caccgccagc acctcgcccg
      301 cgatgcaccc cgtgcgtatg ctcgtcctca cccccacgcg cgaacttgcc gaccaaatcg
      361 accaaaacgt gcagggctac atcaaaaacc tgccgctgcg ccacaccgtc ttgttcggcg
      421 gtatgaatat ggacaaacag accgccgacc tgcgtgccgg ccgcgaaatc gtcgtcgcca
      481 ccgtcggacg gctgctcgac cacgtgaaac agaaaaacat ccatttgaac aaagtcgaaa
      541 tcgtcgtttt ggacgaagcc gaccgtatgc tggatatggg ttttatcgac gacatccgca
      601 aaatcatgca gatgctgccc cgccaacgcc aaaccctgct cttttccgcc accttctccg
      661 ccccgatacg caaactggcg caagacttca tgaacgcgcc cgaaaccgtc gaagtcgccg
      721 cgcaaaacac caccaacgcc aacgtcgagc agcacatcat cgccgtcgat accattcaga
      781 agcgcaacct gctcgaacgg ctgattgtcg atttgcatat gaaccaggtc atcgtgttct
      841 gcaaaaccaa acaaagcgtc gaccgcgtaa cgcgcgaact ggtgcgccgc aacctgtccg
      901 cacaggcgat acacggcgac cgttcccaac aaagccggct cgaaacactc aacgccttca
      961 aagacggcaa cctgcgcgtc ctcgtcgcca ccgacatcgc cgcgcgcggg ctggacattg
     1021 ccgaactgcc cttcgtcatc aattacgaaa tgcccgccca gcccgaagac tacgtccacc
     1081 gcatcgggcg cacggggcgc gcgggcgcgg acggcgtggc gatttccctg atggacgaat
     1141 ccgaacagaa aatgtttgaa tccattaaag agctgaccgg caacaagctg ctcatcgagc
     1201 gcatcgaggg cttcgagccg caatggtggg aacagggcgg cgcaaaaccg gaaaaacccg
     1261 aaatgcgcga accgagacaa cgcaaccgct acgaatccgc caaagcgcaa cgcgaaaaaa
     1321 acacccggcc ggaaaatgcg gcaaacgatg cgggcgcggc ttgcggaaaa attgccggac
     1381 gcagccgccg aagccgccgg gaacaccgga cgtgcgccct gctccaaccg cgttacggcg
     1441 taaaataacc ctgaaaatca aatgccgtct gaacatttcc cgtttcagac ggcatttttc
     1501 aaaccagact aacgcattgg gcgcaaccgc ccataccgga taaatttctg ccgcaaacag
     1561 tttcagacgg catttgctgc ctgtacaata tagtggatta acaaaaat
//
LOCUS       DAXHNE010000229         1541 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.229, whole genome shotgun sequence.
ACCESSION   DAXHNE010000229 DAXHNE010000000
VERSION     DAXHNE010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.229"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(32..1516)
                     /gene="tldD"
                     /locus_tag="ACFM4Z_002033"
     CDS             complement(32..1516)
                     /gene="tldD"
                     /locus_tag="ACFM4Z_002033"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFC6022001.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQIDQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRESYASAPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
BASE COUNT          275 a          453 c          480 g          333 t
ORIGIN      
        1 cctttcagac ggcatcgtgc cggattccgc gtcagattgc gctgccgccg acggtcagtc
       61 cggcatcgat acgcagggtc ggttgcccca cgccgacggg gacgctctgc ccttctttgc
      121 cgcacacgcc gacaccgctg tcgagcgcgg tatcgttgcc tatcatcgaa acgtgtttca
      181 gcacttcggg accgttgccg ataatggtcg cgcctttgac gggatattgc agcctgccgc
      241 cttccaccca ccatgcttcg gacgcgctga acacgaattt gccgctggta atgtccacct
      301 gtccgccgcc gaagttgacg gcgtaaatgc ctttgtcgat ggacgcgatg atttcttccg
      361 gctcatagct gccgttttcc ataaaggtat tggtcatgcg cggcataggg gcggaagcgt
      421 aactttcgcg gcggccgttg ccggtggatt gcgtacccgt caggcgggca ttggtttcgt
      481 cctgcatata gccgactaaa atgccgtctt caatcaatac ggtgcggcgg gtttcgttgc
      541 cttcgtcgtc gatgttgagc gaaccgcgcc ggccggcaat atcgccctga tcgacgacgg
      601 taacgccttt ggcggcgacg cgctcgccta ttctgccgga aaagacgctg gtccccttgc
      661 ggttgaaatc gccttccaaa ccgtgtccga ccgcttcgtg cagcaacacg cccggccagc
      721 cgttgcccaa aacgacggtc atttcgccgg cgggcgcggg gcgggattcg aggttggtga
      781 gtgcctgttt gacggcggca tcgacaaact gccgcaccaa gttttcatcg aaataagcca
      841 agtcgtagcg tccgccgccg cccgcgctgc cctgttcgcg gcgttcgccc tgtttggcga
      901 tgacggtaac gttcaggcgc accatcgggc ggatgtcggc ggcgtgtttg ccgtccagac
      961 gggcgaggta aaccatatcg tattcgcagg tcaaaccggc catcacttgc acgatgcgcg
     1021 gatcggcggc tttggcgatt gcttccactt tgttcaacag cgcgactttg gcggcggaat
     1081 cgaggccggc aatggggtcg gacgcggaac aaaccggctt gccgcgcgtt tcagacggca
     1141 ttttggcgga caccttgccg cctgccgccc caatcgcgcg gacggcgcgg gcggaacggt
     1201 ttatcgaatc gatgcacagg ctgtcggcgt aggcaaaggc ggttttgtcg cccgaaacgg
     1261 cgcgcacgcc cacgccctga tcgatttgga agctgcccga tttgacgatg ccctcttcca
     1321 aatgccagct ttcataggcg gtgcgctggc agtagatgtc ggcgtaatcg acgtggtgcg
     1381 cgccgatgat gcacaggctt ttggcgagca gttcggggga aaggcggttg gcttcgagca
     1441 gccgcgcctg tacggcggaa taggtcggat gcatagtgtc ggcgcataaa aaatcagggg
     1501 cttgattata cggcatttgt tatatagtgg attaacaaaa a
//
LOCUS       DAXHNE010000230         1522 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.230, whole genome shotgun sequence.
ACCESSION   DAXHNE010000230 DAXHNE010000000
VERSION     DAXHNE010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.230"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(217..1140)
                     /gene="lpxC"
                     /locus_tag="ACFM4Z_002034"
     CDS             complement(217..1140)
                     /gene="lpxC"
                     /locus_tag="ACFM4Z_002034"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFC6022002.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYINEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
BASE COUNT          410 a          365 c          370 g          377 t
ORIGIN      
        1 aaaacagaaa cctcaaatcc cgtcattccc gcgcaggcgg gaatccagac ttgtcggtgc
       61 ggacgcttat cggataaaac ggtttcttga gattccgcgt cctggattcc cactttcgcg
      121 ggaatgacga attttaggtt tctgttttgg ttttttgtcc ttgtaggaat gatgaaaatt
      181 taagttttag gaatttaccg gaaaaaatag aaagcgttat ccacaagttc tgatgttcag
      241 ctcgtgaaat gcgtcgggca aatcatcgct gtcggcaaat tccacccggt cgtaagccgt
      301 ttcgtctgcc aaaaccgcgc gcaagagtgc gttgttgatg gcgtgtcccg atttgtagcc
      361 ttcaaatgcg ccgacaatcg gatgtccgac gatatacaaa tcaccgatgg catcaaggat
      421 tttgtggcgc acaaactcat cgggatagcg caagccttca ggattcagga catccgtgtc
      481 gtcaatcacg atggcgttgt tcaaattgcc gcccaaaccc agattgtggg cgcgcatcat
      541 ttccacttcg tgcataaagc cgaaagtgcg cgcgcgcgcg atttcgttga tgtaggattt
      601 gccggcgaaa tcgatttcaa aagtgggcga gctgcggttg aaaaccggat ggtcgaattc
      661 gatggtcagc gttaccttaa agccgtcata cggcgtaaag cgcacccatt tgcccgcttc
      721 tttgatttcg acaggcttga ggattttcaa aaaacgcttt tgcgcctttt gatcgaccac
      781 gcccgcatct tgcaaaaggt aaataaacgg caggctggag ccgtccataa tcgggatttc
      841 gggcgcgttc agctcaatca gcgcgttgtc gataccgtag gcggacagcg cggacataat
      901 gtgttcgatc gtgccgacac gcacgccttt gtcggtaacg atggtggagg aaaggcgggt
      961 atcgttgatc aaataagggg tcagtttgat ttgttcgccc atctcgccgt ccaaatcggt
     1021 acggcggaag gaaatcccgc tgttttcagg cgcggggtgc agggtcagtg cgacccgttc
     1081 gccggaatgc agcccgactc cggtaacgct gatggatttc gccaaagttc tttgcagcat
     1141 aaaccgctcc cttatcaatg gggtaagttt tggaataata cgataaaacc ggaaaaacag
     1201 gctatgtttt tccatagtat ttgccaatgt atccgttttc aatacgtaag ccgcataaaa
     1261 atgtatagtg gattaacaaa aatcaagaca aggcgacgaa gccgcccctc ccgaaaaacg
     1321 caaaaaatgc cgtccgaaaa cctttcggac agcattttcg cgtgaaccgt cattcccaca
     1381 aggacaaaaa accaaaacat aaaaccaaaa acagaaacct aaaattcatc attcccacga
     1441 aagtgggaat ctagttcgtt gagtttcagt tatttagaat aaattttgaa acttaaatcc
     1501 cgtcattccc acgaacctac at
//
LOCUS       DAXHNE010000231         1505 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.231, whole genome shotgun sequence.
ACCESSION   DAXHNE010000231 DAXHNE010000000
VERSION     DAXHNE010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.231"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            32..1432
                     /locus_tag="ACFM4Z_002035"
     CDS             32..1432
                     /locus_tag="ACFM4Z_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HFC6022003.1"
                     /translation="MTPSALKKTVLLLGTAFAAASVHASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNDRHSFGAGIIAQHTSAELRKYADWGIKSKAEILTAKPPKPNGVAEAAKIQAD
                     GHADVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADGAAAKAMWS
                     TMLAANGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFE
                     KEKTVVKGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMN
                     SLPDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARF
                     KNHADIIGLQYTYKFK"
BASE COUNT          395 a          486 c          360 g          264 t
ORIGIN      
        1 atccaccgtc cgccttttta cggaaaccga aatgacccct tccgcactga aaaaaaccgt
       61 cctgctgctc ggcactgcct ttgccgccgc atccgtccac gcatccggct accacttcgg
      121 cacacagtcg gtcaacgcgc aaagcacggc aaatgccgcc gccgcagaag ccgccgacgc
      181 atcgaccatc ttctacaacc ctgccggcct gaccaaactc gacagcagcc agatttccgt
      241 caacgccaac atcgtgctgc ccagcattca ttatgaggcg gattccgcca ccgactttac
      301 cgggcttccc gtccaaggtt cgaaaagcgg caaaatcacc aaaaccacgg tcgcgcccca
      361 catctacggc gcatacaaag tcaacgacaa tctgaccgtg ggcttgggcg tgtacgtccc
      421 cttcggctct gccaccgaat acgaaaaaga ttccgtgttg cgccacaaca tcaacaaact
      481 cggtctgacc agcatcgccg tcgaacctgt cgccgcgtgg aaactcaacg accgccattc
      541 cttcggcgca ggcatcatcg cccaacatac ttccgccgaa ctgcgcaaat atgccgactg
      601 ggggattaag agtaaagcag agatattgac ggcaaaaccg cccaaaccta acggtgtagc
      661 cgaagctgca aaaattcagg ccgacggaca cgccgatgtc aaaggcagcg attggggctt
      721 cggctaccaa ctggcgtgga tgtgggacat caacgaccgt gcgcgcgtgg gcgtgaacta
      781 ccgttccaaa gtctcgcaca cgctcaaagg cgatgccgaa tgggcggcag acggcgcggc
      841 ggcgaaagca atgtggagta cgatgcttgc agcaaacggc tacacggcga atgaaaaagc
      901 ccgcgttaaa atcgttacgc ctgagtcttt gtccgtacac ggtatgtaca aagtgtccga
      961 taaagccgac ctgttcggcg acgtaacttg gacgcgccac agccgcttcg ataaggcgga
     1021 actggttttt gaaaaagaaa aaaccgtcgt caaaggcaaa tccgaccgca ccaccatcac
     1081 ccccaactgg cgcaacacct acaaagtcgg cttcggcggt tcttatcaaa tcagcgaacc
     1141 gctgcaactg cgcgccggca tcgcttttga caaatcgccc gtccgcaacg ccgactaccg
     1201 catgaacagc ctacccgacg gcaaccgcat ctggttctcc gccggtatga aataccatat
     1261 cggtaaaaac cacgtcgtcg atgccgccta cacccacatc cacatcaacg acaccagcta
     1321 ccgcacggcg aaggcaagcg gcaacgatgt ggacagcaaa ggcgcgtctt ccgcacgttt
     1381 caaaaaccac gccgacatca tcggtctgca atacacctac aaattcaaat aaacggcccg
     1441 ccgtttgaat gtaacaatgc cgtctgaaac agatttccgt ttcagacggc attgttttag
     1501 gctcg
//
LOCUS       DAXHNE010000232         1498 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.232, whole genome shotgun sequence.
ACCESSION   DAXHNE010000232 DAXHNE010000000
VERSION     DAXHNE010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.232"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            80..1417
                     /locus_tag="ACFM4Z_002036"
     CDS             80..1417
                     /locus_tag="ACFM4Z_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC6022004.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTILVALPVQLAEFYIGRTGGKNAVDSFRALRPSTQWLWVGRMGVSACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYVISAVIMPVGALGVA
                     VFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFVNSLDIL"
BASE COUNT          269 a          352 c          400 g          477 t
ORIGIN      
        1 tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta tactatcttc
       61 acatttctta ataaatttta tgagtaacca tacttcttgg tcgtccaaaa tcggtttcgt
      121 acttgctgcg gcaggttcgg ccatcggttt gggcgcgatt tggaaattcc cttatacggc
      181 gggcaccaac ggcggcgcgg tgtttttcct gctgtttttg atatttacta tcttggtcgc
      241 cctgcccgtt cagcttgccg aattttatat cgggcgcacg ggcggtaaaa atgccgtcga
      301 ttccttcagg gctctgcgtc cgagtacgca atggctttgg gtcgggcgca tgggcgtttc
      361 cgcctgcttt attttgctgt cgttttacag cgtggtcggc ggatgggtat taaattatgt
      421 cgtccacagt ttcacggggg cggttcatac cggcgcggac tttgaagcct tgttcggcgc
      481 gacgatttcc aatccggcag gttcgctgtc ctatcaggca ctgtttatgc tgattacggt
      541 ttgggtggtc aaaggcggca tttcagacgg cattgaaaag gcaaaccgtt atctgatgcc
      601 ggggctgttt atcctcttta ttgcgctggc aatccgttcg ctgacgctgc cgggtgcaat
      661 ggagggcgtg tctttcctgc tcaaaccgaa ttggtcgtac tttaaagccg atacgatgat
      721 tacggcttta ggtcaggcgt tttttgcctt gagcatcggc gtttccgcta tgattaccta
      781 tgcttcgtat ttgggaaaag atcaggatat gttccgctcg gggcatacga ttatgtggat
      841 gaacctcttg gtttcgctgt tggcaggttt ggtgattttt ccggcggtgt tcgccttcgg
      901 ttttgaaccg agccaggggc cgggattgat ttttatcgta ttgccggcgg tgtttatgaa
      961 aatgccgttc ggtacggttt tgtttgcggt atttatgctg ctggtcgttt tcgccacgct
     1021 gacttcggca ttttcgatgt tggaaacggt cattgcctca accatccgcc aagacgagcg
     1081 caaacgcaaa aaacatactt ggcttatcgg cacggctatt tttattgtcg gcatcccgtc
     1141 cgcgctgtct ttcggcgtat ggggtgagtt taaggttttc ggcaaaacca tttttgattt
     1201 gtgggactat gttatttccg ccgtcattat gcctgtcggc gcgttgggcg ttgccgtatt
     1261 taccgcctgg attcaggaca agcagtctgt gttaaaagat gccggcgcgg gcagcaccgt
     1321 accacgggca gtgctgctgc tgtggctgaa taccttgcgc taccttgccc cgattgccat
     1381 tattattgtt ttcgtcaatt ctttggacat cctttaaaag ccatccaaac agcaaaaatg
     1441 ccgtctgaaa gcctttcaga cggcattttt gcttcaggtt cagcctattt cgttcaaa
//
LOCUS       DAXHNE010000233         1454 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.233, whole genome shotgun sequence.
ACCESSION   DAXHNE010000233 DAXHNE010000000
VERSION     DAXHNE010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.233"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(401..712)
                     /locus_tag="ACFM4Z_002037"
     CDS             complement(401..712)
                     /locus_tag="ACFM4Z_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6022005.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(709..1077)
                     /locus_tag="ACFM4Z_002038"
     CDS             complement(709..1077)
                     /locus_tag="ACFM4Z_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFC6022006.1"
                     /translation="MRIFKNQWIVKFAKKHKINDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILLKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
BASE COUNT          276 a          400 c          331 g          447 t
ORIGIN      
        1 aaaaacgtcg gcgttaaggg aaggggatcg agaatttgag ccgttgtttc aaaatgccgt
       61 ctgaaatcgt ttgagattca gacggcattt aaacagttct gcgccccgcc cgttttgcct
      121 tcaagcccgc gccgcttaaa tccaatcccc cctgcaacgg gcgcaaaaaa atccccgtgc
      181 ctcgcggtcg gggatgtttc gggtttcgcg cccgtctgcg ccgtgccttg ctgtcgggct
      241 atgtcgtctg caccgccgcc gtttcggctt ggtttgatgc ggtcggggca tttgtgcggc
      301 tacgccctga tattcagttc ggcggtcttg cctgttttgg caggcatatt cagcagcgcg
      361 tctattgtga acttgctgat tttgccgttt gccaagtcgg ctacgcaatg gcttcgatac
      421 ccttgttttt gacgatggtc agcagcttca acgccgcgcc gctgggcttt ttaacgcccc
      481 gctcccaagc cgaaacgtga ttttttccca cgttgagata gatggcgaaa gcggcttgcg
      541 atagtgcctc cttctccctg attgccttga tgtctccgcc gctcaacggt ttgatttcgg
      601 tcaggcatga cttgtcaaag ccgcgcatag tctttttgtc gattgcgccg atgttgtaag
      661 ccccttccat catttcatga atggcggcta atgcctcgct tttgtatttc atgattctat
      721 ctccgtgata atgccgtctt ctttcaaagc cgccagctct gcccgcgtgt atttcagata
      781 atatgcggcg gcttttcggt aaacgtccag ttctttatcc gaaatgtttt ccctgtcgtt
      841 cttggcaaag gcgtaaacaa aaaatgccct gtctgcctgt ttgagcagta tcaggctgcg
      901 atagccgccg cttctgcctt gtccttgcct tgctatgcgc tgcttaatca caccgccgcc
      961 taaatctgcg tctatcagcc cgttatccgc ccgctctacc gcttccagca gctcggaatc
     1021 gttgattttg tgcttcttgg caaatttcac tatccattgg tttttgaata tccgcattgt
     1081 gtagccgttg attagtgata cttggaatta taccagagac agacaacacg gcaagctgcc
     1141 ttttttatgc cccgcaattt ctccgtttat gccgcctgaa acaccggcag ctaacctgat
     1201 tattttttaa tcaatcgctt ttaatggctc aaattgcgtt tttagcgcgt ttcgctgtct
     1261 tcctatgccc gtatataacc aagcggttta accaccattc ctgcctcaaa ataggcgcgg
     1321 ttccgtgcgt tttggctttt cattccgcta cgctcccact gccaagacgg tgaaaaaccg
     1381 gaaaacccca aaagttgtta ccgattcgtt accatttgtt accgcatttg ttaccaattt
     1441 tttatctttt tctt
//
LOCUS       DAXHNE010000234         1454 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.234, whole genome shotgun sequence.
ACCESSION   DAXHNE010000234 DAXHNE010000000
VERSION     DAXHNE010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.234"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..143)
                     /locus_tag="ACFM4Z_002039"
     CDS             complement(<1..143)
                     /locus_tag="ACFM4Z_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6022007.1"
                     /translation="MSTFFQQTAQAMIARHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLR"
     gene            complement(205..876)
                     /locus_tag="ACFM4Z_002040"
     CDS             complement(205..876)
                     /locus_tag="ACFM4Z_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6022008.1"
                     /translation="MFPDKYKLSLKENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
BASE COUNT          355 a          321 c          345 g          433 t
ORIGIN      
        1 tctctaaggt aacgggtttt ttgacggttc aggtactgtt cgatcggctg ccaatcaatc
       61 acttgatcca acttcaatag tgggaaacgg tcgatgtgtc tggcaatcat ggcttgggcg
      121 gtttgctgga agaaggtgct catggaacat cccctaaatg tcttggtggg aatttagggg
      181 attttgggga attttgcaaa ggtctcagtc tatgcccgat atacaatttt gatacacaaa
      241 cttggaaata tcggtatcgt cgccggagcg atagaatgcg gacagttttt cattgaattg
      301 cggcatctgc atttcggaga tttccaagat gccgcagccc cccgccatca tcagaccgtt
      361 ggcaaataag gttgccgttc gtttgttgcc gtcccaaaaa acctgctgcc gcatacaata
      421 aagcataaaa cggacggctg cctccgtcgt cgaaccgctt tgcagtccga tattttgtaa
      481 cacgcgggcc acttcaattt ccttcactgg attcggggca tgacgggaac cgtccaatag
      541 cgttacgccg accgaaccgg tacggaaatc accgggtgcc aaagaatcgt ccttggcaac
      601 aatgttgttg atttttttaa ggagtgaaat atcgaccggt tcgccgtttg aaatatgcga
      661 aatcacatat tgataggcac gtttcaggtt caagatggtt tggatgtctt ccagtgacgc
      721 ggaggctaca tttttgccat ggataatttg ttcggtctgc aacaaggtcg tctgacaatt
      781 ctcaaaacgg ctgaggttgt gaatttgggc aaccaatact ttctttgcca aaaaaatatt
      841 ttctttcaaa ctcaacttgt atttgtcggg aaacacgata attccttaaa taattaaagc
      901 agtacgttaa atcatcatct tatcatcact ttcggggttt atgttgcgtg cgtccgtttt
      961 ctaatacggt ttcccatccc gcgaaacgag gcggaggcct gtgcaaaaaa cctgttcacg
     1021 aaattttccg cattaacgat acgctgaaaa tgcgctaaaa atgactgtgt ttgaatatcg
     1081 gttgatttca tccgtttgtg taacctcgcg ccggttttgt catgattttg cattatagtg
     1141 aattaaattt aaaccagtac agcgttgcct cgccttaccg tactatctgt actgtctgcg
     1201 gcttcgtcgc cttgtcctga tttttgttaa ttcactatat ttttgtcatg cggatatttc
     1261 acgggatgac aaaacgggcg caaaaaagcc cgattggaaa atccgaatcg ggcttttttg
     1321 tgatgttctg ttcgatttgc gtactaactg ccggccggag cgtccgcgcc cggtttggca
     1381 ggtcggattc tcgaatccga cggctatttg agatggcagg cttttgtcgg atacaagtat
     1441 ccgacctacg gctt
//
LOCUS       DAXHNE010000235         1435 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.235, whole genome shotgun sequence.
ACCESSION   DAXHNE010000235 DAXHNE010000000
VERSION     DAXHNE010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.235"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            128..1258
                     /locus_tag="ACFM4Z_002041"
     CDS             128..1258
                     /locus_tag="ACFM4Z_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC6022009.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYRIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDKSRGGRGDLERLAARDIIRFPGFVNNVSEVIKEH
                     HIFVLPSYYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAE
                     KMIYFIENREAIRLMGNASYAIAKDKFDAEKVDLKLLDILKA"
BASE COUNT          388 a          339 c          336 g          372 t
ORIGIN      
        1 ggcaacgccg taccggtttt tgttaatcca ctataatacg caggcaaacc caagccttgc
       61 tgggttacag gatgaaaccc tgccgtagca gaccgcattt ccaatccata ttcaaggtgt
      121 ccgccttatg aaaatcgttt ttatcacaac agtcgcatcc agcatttacg gtttccgcgc
      181 ccccgtcatt aaaaaattaa tcggcaaaaa ccatcaggtg tatgcctttg tatcggagtt
      241 ttccgataat gaattggata ttatcaggga aatgggggtt acacccgtta cctaccgttc
      301 aaaccgcagc gggctgaacc cgttttcgga tataaaatcc accttcctca tctttaaaga
      361 actcaaaaaa atatcgccgg atttggtttt cccttatttc gcaaaacccg tgattttcgg
      421 cacttttgcc gcaaaactgg caggcgtgcc cagaatcgtc gggatgctgg aaggtttggg
      481 attcgcattt accccgcagc cggaaggcat accgttaaaa acaaaaatca taaaggggat
      541 tttgattgcc ttataccgca ttgccctgcc gatgttggaa agcctgattg tattaaaccc
      601 cgacgacaaa gacgaactga cggacaaata cggcatcaaa ataaaaaaca tccatatttt
      661 gggcggaatc ggtctggatt tgcggcaata tccttattcc gaggcggata ttcccgatga
      721 aaaagaaccc gtaaaatttc tctttatcgg cagatttctg aaagaaaaag ggattgatga
      781 ttttattcgg gcggcggaac aggttaagga caaatacccc gatacggttt ttaccgcttt
      841 gggcgcaatc gacaaatcac gcggggggag gggcgatttg gaacggcttg ccgcccgcga
      901 tattatccgt ttccccggtt ttgtgaacaa tgtttccgaa gtgataaaag aacatcatat
      961 attcgtattg ccgtcttatt atagggaagg cgttccccga agcactcagg aggcaatggc
     1021 cgtcggcagg gcggtgatta cgacggatgt ccccggatgc agggaaacgg tcgccgacaa
     1081 ggtcaacggc ttcctgatcg aaccttggaa tccccgcatc ttggccgaaa aaatgattta
     1141 ttttatcgaa aacagggaag ccatccgcct gatggggaat gcaagttatg cgattgccaa
     1201 agataaattc gatgccgaaa aagtcgattt gaaattactc gatattttga aggcgtaaac
     1261 aaggctgccc gcttttgagt ttcggtcatt tctgataaac ccccgtcatt cccgccacac
     1321 ccccgtcatt ccctcgaaag cgggaattca ggttcgttta gtttcggtca tttccgataa
     1381 attcttgcaa ctttgcgttt ctagattccc actttcgtgg gaatgacggc ggagg
//
LOCUS       DAXHNE010000236         1417 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.236, whole genome shotgun sequence.
ACCESSION   DAXHNE010000236 DAXHNE010000000
VERSION     DAXHNE010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.236"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(34..492)
                     /locus_tag="ACFM4Z_002042"
     CDS             complement(34..492)
                     /locus_tag="ACFM4Z_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6022010.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     SYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
     gene            complement(1056..1259)
                     /locus_tag="ACFM4Z_002043"
     CDS             complement(1056..1259)
                     /locus_tag="ACFM4Z_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6022011.1"
                     /translation="MLSLEIQQEGFTIGGGLVLGGCAGAHLARKEPLILTGKTGAGAS
                     AIANASIGYQWTVNLSKPKEGAK"
     gene            complement(1225..1417)
                     /locus_tag="ACFM4Z_002044"
                     /pseudo
     CDS             complement(1225..1417)
                     /locus_tag="ACFM4Z_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          490 a          259 c          169 g          499 t
ORIGIN      
        1 caatcgattc cgttgtattt tcgcaagaca cgtttagtct agagtctgta tattacattt
       61 atttttaggg tctgctagcc aatttcttgt tcccttcatt attttatctt ctgaaagaaa
      121 attatttttt tccatgctat taatattaat gatatgattt ttatttaaaa taatttttcc
      181 atatgaaatt tccggtttgt aatagcaaga gtctgaagca aatccttgcc cgtcaatata
      241 agaaaaataa atgttatcat cctccttata atattctaaa accaatccgg atattatttt
      301 atcaaaattc ccttcatttc catctttcat gactaaaata taattagcag gccaaccttt
      361 atcgtaaaca tattcaaact gatagttccc gaaactctcg atatccgaac taaaaaagaa
      421 gaaaagcaaa ttaatagcca atacaaaaac aaataagaac aaaaatagca aaattttcaa
      481 cttagttaac aatatttacc tctcctttaa attcaatcct gaaaggtacc ccttaccctg
      541 ggctaccaat tatagtttcc atatttctaa atattgtttt tacattactt ttttctcccc
      601 ccaaaggaat gcattttaaa atcatgcttt tcaggtgcta atcgatactt accattaaca
      661 tctttaatca cagatatatt tccatgtata gcccaacgtg aaaaatctga gtattatata
      721 cagttatacc ttactgaaac aaagctagct gtacatatct gtaccatatt tttactaaaa
      781 acaaaaaata tttataatta ttaaggataa gataaaaacc cccaacgcca gattaattct
      841 taataatttc aaataaaaaa tatttgagat tttttatttt ccaaataata tattgaaata
      901 tttaaagtta aggtagcttg atttaattag aaaatcaaaa aaaccattaa ttaatttttc
      961 ttctttaaat attttatatg atattataaa attaaataac attgcgaaaa catatgaaat
     1021 catcaaaata ccaaatatat agtgggaatg cattattatt tagctccttc ttttggcttt
     1081 gacaaattga cagtccattg atatccaatg cttgcatttg caattgctga cgcacctgcc
     1141 cctgttttcc ctgttagtat caatggttct tttcttgcaa gatgtgcacc tgcacaacca
     1201 cctaatacta aaccaccacc tattgtaaat ccttcctgtt gtatctctaa tgataacatt
     1261 ttcttcttgt gccgccctta atacagattg tggaattgga ttgacctgag caggaacttc
     1321 taaaataagt tttcctgtaa gttttcttcc tcttagcgga gcaggtgtac ttggaacatt
     1381 ggaaatgaca aagtgagtac gcaaaccacc tgaaggc
//
LOCUS       DAXHNE010000237         1324 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.237, whole genome shotgun sequence.
ACCESSION   DAXHNE010000237 DAXHNE010000000
VERSION     DAXHNE010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.237"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(333..743)
                     /locus_tag="ACFM4Z_002045"
     CDS             complement(333..743)
                     /locus_tag="ACFM4Z_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6022012.1"
                     /translation="MKIYNLDNIITSSALSLYKSTWVKLDLISCAKFIIKGTDFTFDW
                     DEGIENWIVFFHKNKVIGYLCAKLPFAILDNEQLKNQLYHKYPHVLTLQIKNIETEKI
                     SLNVNICRLTLCPSFHDEHDTLVCSVQDFYVNTV"
     gene            complement(747..>1324)
                     /locus_tag="ACFM4Z_002046"
     CDS             complement(747..>1324)
                     /locus_tag="ACFM4Z_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC6022013.1"
                     /translation="VAEAAGTVWRGKKVELNPTKWDWVKNTGYKKPAARPMQTLDGEM
                     AGGNKPAKQATPNGKAGVSTQQSLQNSLVPQKNYTHTTYNGGSAITDGKYITDPLSMV
                     RHKPDASSLDKIDSKSLFKSDVDAEKATLDAARYADKHNLWDSQNKAKVPTNDIVGYT
                     DGKPTHIINIYRRKPNKDGFRPVHGTPGKER"
BASE COUNT          385 a          251 c          215 g          473 t
ORIGIN      
        1 ttgtatccga caaaaatatt tgacgcgtct atcgtttccg aaatgccgct gttgaaaaac
       61 gtcggattcg agaatccgac ctacggttgc tgaacaatgt tttgtagaac ttgctcaaaa
      121 tccatgtttc gccattttcc tttatatata cctaatgcag cacgggttac gatttcgttt
      181 aagtcataaa tagccattta cgtcctctgc taattttcag cgagtagatt gggttaccaa
      241 acccaacaaa aaagcagcct gaaaaatttc aggatgcttg ttttatgttt tttatcagtg
      301 aattaggttt taatccagcc tgcgcttgct agttaaacag tatttacata aaaatcttgc
      361 actgaacaaa caagcgtatc atgttcatca tgaaatgatg ggcataacgt taatctacaa
      421 atgtttacat ttaaagatat tttttcagtt tcaatatttt taatttgtaa ggttaataca
      481 tgaggatatt tgtgatatag ctgatttttt aattgttcgt tgtctaatat agcaaaaggt
      541 aattttgcac acaaataacc aataactttg tttttatgaa aaaaaactat ccaattttca
      601 attccttcat cccaatcaaa agtaaaatcc gttcctttta tgataaattt agcacaagat
      661 attaaatcta acttaaccca agtggattta tacagactca atgctgatga agttataata
      721 ttatctaaat tataaatttt cataaatcaa cgttccttac ctggtgttcc atgcacaggt
      781 ctaaacccat ctttattcgg ctttctacga taaatgttaa ttatatgtgt cggttttccg
      841 tctgtatagc caactatgtc gttcgttgga acttttgcct tattttgact atcccataaa
      901 ttatgtttat ctgcatatct agcagcatct aatgtagctt tttccgcatc aacatctgat
      961 ttaaataaac ttttgctatc tatcttgtct agactagatg catcaggttt gtgtcgtacc
     1021 atactgagag ggtcagtaat atatttacca tctgtaatag ctgatccacc attataggta
     1081 gtatgcgtat aatttttttg tggaacgaga ctattttgca aagattgttg tgtagaaacg
     1141 ccagctttgc cattaggagt agcttgcttc gctggcttat tcccaccggc catttcccca
     1201 tctaaagtct gcatagggcg ggcagcaggt tttttataac cggtattttt aacccaatcc
     1261 catttagtcg ggttaagttc tactttttta cctctccaaa ccgtacctgc ggcttctgct
     1321 acgg
//
LOCUS       DAXHNE010000238         1288 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.238, whole genome shotgun sequence.
ACCESSION   DAXHNE010000238 DAXHNE010000000
VERSION     DAXHNE010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.238"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..525
                     /locus_tag="ACFM4Z_002047"
     CDS             <1..525
                     /locus_tag="ACFM4Z_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HFC6022014.1"
                     /translation="EAGAGIATGAVTVGNAWEAPVGALSKAKAAKQAIPTQTVKELDG
                     LLQESKNIGAVNTRINIANSTTRYTPMRQTGQPVSAGFEHVLEGHFHRPIANNRSVFT
                     ISPNELKVILQSNKVVSSPVSMTPDGQYMRTVDVGKVIGTTSIKEGGQPTTTIKVFTD
                     KSGNLITTYPVKGN"
     gene            532..1041
                     /locus_tag="ACFM4Z_002048"
     CDS             532..1041
                     /locus_tag="ACFM4Z_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6022015.1"
                     /translation="MSNFEKKYILELNDALSHLNHNSTSFDLLKVLISWLSNDIVIDK
                     FKILGYDFSKYIEMNPDDYPVEKSILNREEIIYLKNNIYRKISSGNFKFQYFVQYIRD
                     ILEYLFIEHIERVCPYCEWGEMQKLEEQNTHETVYLCTQCGCAFYNDNSQFLLKTPLT
                     IPMKRDEFK"
BASE COUNT          425 a          239 c          249 g          375 t
ORIGIN      
        1 gaagcgggcg cgggaatcgc cacgggtgcg gtaacggtag gcaacgcttg ggaagcgcct
       61 gtgggggcgt tgtcgaaagc gaaggcggcc aagcaggcta taccaaccca gacagttaaa
      121 gaacttgatg gcttactaca agaatcaaaa aatataggtg ctgtaaatac acgaattaat
      181 atagcgaata gtactactcg atatacacca atgagacaaa cgggacaacc ggtatctgct
      241 ggctttgagc atgttcttga ggggcacttc cataggccta ttgcgaataa ccgttcagtt
      301 tttaccatct ccccaaatga attgaaggtt atacttcaaa gtaataaagt agtttcttct
      361 cccgtatcga tgactcctga tggccaatat atgcggactg tcgatgtagg aaaagttatt
      421 ggtactactt ctattaaaga aggtggacaa cccacaacta caattaaagt atttacagat
      481 aagtcaggaa atttgattac tacataccca gtaaaaggaa actaactaaa tatgagtaac
      541 tttgaaaaaa aatatatttt agaattaaat gatgctttaa gccatttaaa tcataactct
      601 acctcatttg atttattgaa agttttgatt tcatggttat caaacgatat tgtcattgat
      661 aaatttaaaa ttttaggtta tgactttagt aaatatatcg aaatgaatcc cgatgactat
      721 ccggttgaaa aatctatatt gaatagagag gaaattattt atctcaaaaa caatatttat
      781 cgtaaaatat catcaggaaa ttttaaattt caatactttg tacaatatat tagagatatt
      841 ttagaatatt tatttattga acatattgaa agagtctgtc cttactgcga atggggtgaa
      901 atgcaaaaat tagaagaaca aaatacgcat gaaacggtgt atctctgtac tcaatgtgga
      961 tgtgcttttt ataacgataa ttcacaattt ttattaaaaa cccctttaac cattccaatg
     1021 aaacgtgatg aatttaaata aacaagccgt agcctgcatg aaccctaaaa tccacgtgta
     1081 gcgtgtgtgc gccagcacgc atgcgttcca tgatttacgg ctcaatgccg tctgaaaagc
     1141 tcacaatttt tcagacggca tttgttatgc aagtaaatat tcagattccc tgtatgctgt
     1201 acagacgcgg gagtgttaag ccccccttgt ttgaagctcc gcggctcctg ccgagcttca
     1261 ccgaccccgt tgtgcccaag ctctctgc
//
LOCUS       DAXHNE010000239         1285 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.239, whole genome shotgun sequence.
ACCESSION   DAXHNE010000239 DAXHNE010000000
VERSION     DAXHNE010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.239"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            31..543
                     /gene="coaD"
                     /locus_tag="ACFM4Z_002049"
     CDS             31..543
                     /gene="coaD"
                     /locus_tag="ACFM4Z_002049"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFC6022016.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            575..1231
                     /locus_tag="ACFM4Z_002050"
     CDS             575..1231
                     /locus_tag="ACFM4Z_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HFC6022017.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNKESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRCGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFDYQNRQIEIVAPLKNEWQTENIHRALEEFCMENKID
                     "
BASE COUNT          383 a          321 c          301 g          280 t
ORIGIN      
        1 acagccggct atgaaaccgg gaggaatcaa atgttaccga atacaccgcg ccgcgccgtt
       61 tatgccggca gtttcgatcc gcccacattg gggcatctgt ggatgatacg gcaggcgcaa
      121 tctatgtttg acgaactcat cgtcgccatc ggcattaacc ccgacaaacg cagcacctat
      181 accgtcgctg aaaggcagga tatgttgtgc gatattacta aaatgtttcc caacgtcaga
      241 accgatgtat ttgaaaaccg atttctggtg cattacgccc gtgaggtaga tgcaggattc
      301 atcgtgcgcg gcatccgttc tgcttcggat tacgaatacg aacgttccat gcgccatatc
      361 aacagcgacc tcgcccccga aatatccacc gtattcctca tgccgccgcg cgaaatcgcc
      421 gaagtgtcgt ccactatggt caaaggactg gtcgggccgg aaggctggac ggaaacgatc
      481 caccgctacg tgccgcaagc tgtgtacgaa aaaatccttg ccgaacatca acacgaaaat
      541 tgaacccttc tatttttgca aggcatatat ggatatgttg gaaattttgt tccgccatca
      601 agacttcgtc gccatcaaca aaccgggcgg catatccgtc caccaagaca gcggcgagac
      661 cggactggcc cgaacactcg ccatacagtt gggcgtagag cgtgtgtggc tgctgcaccg
      721 attggacaaa cagaccagcg gcattttact gtttgccctc aacaaagaaa gcgcgtccgc
      781 ccttagcgga cagtttgccg gcaaaagcat caaaaaaacc tatttggcac tgtccgaccg
      841 caagccgtcc aaaaagcagg gttggattaa aggcggaatg gaaaaatcca gatgtggaat
      901 gtggaagttg acgcgcaata cagaaaatat cgccgttacc cgattccaca gcatcagtat
      961 cgccgaaaaa ctgcggctgt tcatcctaga accgcatacg ggcaaaacgc accaattgag
     1021 agtggcgatg aaaagtttgg gcagtccgat tctgggcgac agcttatacg gcggaacaga
     1081 atccgaaacc atgtttctgt atgcatggaa aatacaattt gactaccaaa accgacaaat
     1141 cgaaattgtt gcacccttaa aaaacgaatg gcagactgaa aatatccatc gggcactgga
     1201 agaattctgt atggaaaata aaattgactg atataaatta aatttataaa caaatagata
     1261 aatattttta aaaaaatttc ttcag
//
LOCUS       DAXHNE010000240         1283 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.240, whole genome shotgun sequence.
ACCESSION   DAXHNE010000240 DAXHNE010000000
VERSION     DAXHNE010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.240"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..>1283
                     /locus_tag="ACFM4Z_002051"
     CDS             <1..>1283
                     /locus_tag="ACFM4Z_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6022018.1"
                     /translation="IPKGNLKTEIEKLSKQPEYAYLKQLQTAKNVDWKQVQLAYDKWD
                     YKQEGLTGAGAAIVVIIVTALTYGYGAAAAGSVTAAGSSTAAAATTTAAATTTVSTAA
                     AMQTAALASLYSQAAVAIINNKGDVGKALKDLGTSDTVKQIVTSALTAGALNQMGADI
                     AQLNSKVRTELFSSTGNQTIANLGGRLATNLSNAGISAGINTAVNGGSLKDNLGNAAL
                     GALVNSFQGEAASKIKTTFSDDYVAKQFAHALAGCVSGLVQGKCKDGAIGAAVGEIVA
                     ESILGGRNPATLSDAEKHKVISYSKIIAGSVAALNGGDVNTAANAAEVAVVNNALNFD
                     STPTNAKKHQPQKPDKTALEKIIQSIMPAHAAGAMTNPQDKDAAIWISNIRNGITGPI
                     VITSYGVYAAGWTAPLIGTAGKLAISTCMANPSGCT"
BASE COUNT          376 a          308 c          337 g          262 t
ORIGIN      
        1 attccgaaag gcaatctgaa aaccgaaatc gaaaagctgt ccaaacagcc cgaatacgcc
       61 tacctgaaac agcttcagac ggccaagaac gtcgattgga aacaggtgca gctggcctac
      121 gacaaatggg actataaaca ggaaggcttg accggagccg gtgcagcgat tgtggtgatt
      181 attgtaaccg ctctaactta tggatacgga gcggctgcag cgggtagcgt aactgccgca
      241 ggaagtagta cagccgcagc tgcaacaacg acagcggcag caactaccac tgtttctact
      301 gcagctgcca tgcaaaccgc agctttagcc tccttgtata gccaagcagc tgtagccatc
      361 atcaataata aaggcgatgt aggcaaagca ttaaaagatc tcggcaccag tgatacggtc
      421 aagcagattg ttacttccgc actgacggcg ggtgcattaa accagatggg agcagatatt
      481 gcccaattga acagcaaggt aagaaccgaa ctgttcagca gtacgggcaa tcaaaccatt
      541 gccaaccttg gaggcagatt ggccaccaac ctcagcaatg caggtatctc agctggtatc
      601 aataccgccg ttaacggcgg cagcttgaaa gacaacttag gcaatgccgc attgggagca
      661 ttggttaata gcttccaagg ggaagccgcc agcaaaatca aaacaacctt cagcgacgat
      721 tatgttgcca aacagttcgc ccacgctttg gcgggttgtg ttagcggact ggtacaggga
      781 aaatgtaaag atggggcaat tggcgcagca gttggggaaa tcgtagctga atccatactt
      841 ggcggcagaa accctgctac actcagcgat gcagaaaagc ataaagttat cagttactcg
      901 aagattattg cgggcagcgt ggcggcactc aacggcggcg atgtgaatac tgcggcgaat
      961 gcggctgagg tagctgtggt gaataatgct ttgaattttg acagcacccc taccaatgcg
     1021 aaaaagcatc aaccgcagaa gcccgacaaa accgcactgg aaaaaatcat ccaaagtatt
     1081 atgcctgcac atgcggcagg tgcgatgact aatccgcagg ataaggatgc tgccatttgg
     1141 ataagcaata tccgtaatgg catcacaggc ccgattgtga ttaccagcta tggagtttat
     1201 gctgcaggtt ggacagctcc gctgatcggt acagcgggta aattagctat cagcacctgc
     1261 atggctaatc cttctggttg tac
//
LOCUS       DAXHNE010000241         1241 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.241, whole genome shotgun sequence.
ACCESSION   DAXHNE010000241 DAXHNE010000000
VERSION     DAXHNE010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.241"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            816..>1241
                     /locus_tag="ACFM4Z_002052"
     CDS             816..>1241
                     /locus_tag="ACFM4Z_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC6022019.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     LLAEGQKSAVTEYYLNHGEWPGDNSSAGVASSSTIKGKYVKEVTVANGVITATMLSSG
                     VNKEIQGKKLSLWAKRQDGSVKWFCGLPVTRTDAKADTVA"
BASE COUNT          392 a          358 c          230 g          261 t
ORIGIN      
        1 ccggttcggt aaaatggttc tgcggactgc cggttacgcg cgccgacaac gccaaagacg
       61 acgccgttac cggcaccgac aaaatcgaca ccaagcacct gccgtcaacc tgccgcgatg
      121 attcatctgt cgtttgcata gaaacaccac ctacggcttt ctataaaaat acctaaaccg
      181 tcattcccac gaacctacat cccgtcattc ccacgaaagt gggaatccag aacgcaaaat
      241 ctcaagaaac ctttttaccc gataagtttc cgtgccgaca gacctggatt cccgcttttg
      301 cgggaatgac gaatccatcc gcacggaaac ctgccgcgat gattcaactg ccggttgcac
      361 aggaacacca cgcgccgact tcaaacactt tcagaaaacg gaaattttta aaaattaaat
      421 ttaaaatttc ccacccaacc caccctatgc taccgcgcaa attcaaaaat ctcaaattcc
      481 gacccaatca acacacccga tgccccatgc caatagagat accccacacc aataaaaaag
      541 tgccgaaaat cggcactaaa actgacaatt ttcggcatta ctgacaaaaa acggtaaccc
      601 cccgcttcct caaaccacac ccaaacgaaa gaaaatgtaa aataaaaaca accacataaa
      661 gataaacgca taaattttca cctcaaaaca caaacttgat accaatcttg ctttataata
      721 cacaggtatc gcaacaaaat accgatggtc aaatacattg cataatgccg atggcgtaag
      781 cttgaggcat ttcctttcca attaggagta attttatgaa cacccttcaa aaaggtttta
      841 cccttatcga gctgatgatt gtgattgcca tcgtcggcat tttggcggca gtcgcccttc
      901 ctgcttatca agactacaca gcccgcgcac aagtttccga ggccattctt ttggccgaag
      961 gtcaaaaatc agccgtcacc gagtattacc tgaatcacgg cgaatggccc ggcgacaaca
     1021 gttctgccgg cgtggcatct tcttcaacaa tcaaaggcaa atatgttaag gaagttacag
     1081 tcgcaaacgg cgtcattacc gccacaatgc tttcaagcgg cgtaaacaaa gaaatccaag
     1141 gcaaaaaact ctccctgtgg gccaagcgtc aagacggttc ggtaaaatgg ttctgcggac
     1201 tgccggttac gcgcaccgac gccaaagccg acaccgtcgc c
//
LOCUS       DAXHNE010000242         1232 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.242, whole genome shotgun sequence.
ACCESSION   DAXHNE010000242 DAXHNE010000000
VERSION     DAXHNE010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.242"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            40..372
                     /locus_tag="ACFM4Z_002053"
     CDS             40..372
                     /locus_tag="ACFM4Z_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6022020.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDMPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            704..1213
                     /locus_tag="ACFM4Z_002054"
     CDS             704..1213
                     /locus_tag="ACFM4Z_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFC6022021.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT          329 a          231 c          293 g          379 t
ORIGIN      
        1 ccttgttttg acggaaaacc atcatataaa ggaacactta tgcagatttt atcttttcaa
       61 ccggacattg cggaacgtat gctggaaggt acggaaggcg agtcggtcaa cgaaaacgca
      121 caattcgtcc gtacggacaa cggctattgg attgcgtggc atgaaggcgt agcggcactg
      181 cttgcgcccg atatgccgcc gggcattccc tgtttttggg tggaaggcgc ggaaagcctt
      241 gaagagttgt gcgtcatggt ggaacgcggc gagtttgacg aagtggaaga gtttgacggc
      301 gatgacgacg aatggctcga aacggcacag ggttgcgggc accacggcga cgcttgcgcc
      361 tgcggacatt aaaggcattg caggcttgcc gcaagggggg caaggctttg ccgtttttta
      421 aataatcggt ggattgcatc tgtttgcaat gggctgaacg cttccctttg tattttattg
      481 aataaaaaaa cttatcttgg tattataatt aaggcagcat caattatttt gggatggcaa
      541 taaacgcaaa gcattgattt gcgccgattg cagacttatt atagcaggtt gcggcgcgga
      601 cttaatggga attgtttata gagcatgatg gtctgaaaca tcattaatta aaaataccat
      661 taatccgatt tatatttatt tcaatttcaa tggaaaaaca tcaatgacaa tgattttaag
      721 cattttaagc ctgtttttta tcatcagact gttattttta gccgtctcta ttaaacatga
      781 aaaagccttg attgccaaag gggcaaaaca atacggaaaa accaattcca cgctgcttgc
      841 ggcagttcat acgctttatt atttggcgtg ttttgtttgg gtatggcttt ctgacactgc
      901 ttttaatggc atatccttga ttggtacgct gacggtgatg gcttcgtttg tgatattgtc
      961 attgattatt aagcagttgg gggagatttg gacggttaaa atctatattt taccaaatca
     1021 tcaaattaat cgttcgtggt tgtttaaaac attccgccac cccaattatt ttttaaacat
     1081 catacccgaa ctgattggca tcgccttatt atgtcaagcg tggtatgttt tattgattgg
     1141 cctgcccatt tatttgctgg tcttatttaa gcgtatccga caagaagaac aggcgatggc
     1201 aacacttttt taacccgttt catcaattat ag
//
LOCUS       DAXHNE010000243         1175 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.243, whole genome shotgun sequence.
ACCESSION   DAXHNE010000243 DAXHNE010000000
VERSION     DAXHNE010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.243"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(233..1087)
                     /locus_tag="ACFM4Z_002055"
     CDS             complement(233..1087)
                     /locus_tag="ACFM4Z_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC6022022.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIAAFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT          336 a          349 c          305 g          185 t
ORIGIN      
        1 aatcccgtca ttcccgcgca ggcggaaatc cggacatcca acgctgcagc aatttgtcgg
       61 aaataaccga aactcaaaaa accggatgcc cgcctgcgcg ggaatgacga cgcaaagatg
      121 gtcgttattt cggataaata tccgtctttt cgttccggat acccgctttc gcaggaatgg
      181 agatggaaag atttcagcct attcggcaat catcaaatac cgcttcccaa ctttattttt
      241 gatgcgacag ccggccggca accaaggtgg cggcgatgac gacaaacacg cccaaggcgg
      301 acacgggcga caggtgttcg cccaaaatca aaaccgccag cagcacgccg acgacgggtt
      361 cgagcgaaat caacagtccc gaaacattgg caggaacacg gctcatcccc ttgttccaca
      421 gccaataggc gtaccagccg caccccaaac ccaaatacag cagcgacaat accatcccga
      481 cgctccagtc cacggtataa ctttgcgcca aagcaagcga aaacggcagg cacatcaacg
      541 atgcggcggc aatggaaaca gatgtgaatg ccggtgcgcc gatgcgtgca atcagccttt
      601 gcgtcggacg catagcggca caaaagcccg cgcccgccaa caacaccagc aggcagccga
      661 accagccgac ttcgccgccc tcttccgcac cgcccgccat cagcagcgcg acaccggcaa
      721 atgccgccgc gccgcatatc cagtggtagg cacgcgcttt gtcgttgaag aaaaagtgtc
      781 cgacaaacac catcagcagt ggctcgagtc cgacaatgac cgatgcgctg gcggcggaag
      841 tgtatttcaa cccgacaaac tgaagcagca gggtcagcac atagttgaca aacgccgcaa
      901 tcagcaacgg cttccattcc tcacgcggaa tcttgccgac atgacggcgg caggcgggca
      961 gtgcaggcag cgcggcaatt agcaggcgca cgccgaccat caatgcggga tcgatgccgc
     1021 catagacata tttggcggca ataaacgagc tgctccagat aatcagggca aggatttggt
     1081 aaaacatagt gagtgataaa gtcagatggt catacaaaag tatagcggat gaacaaaaat
     1141 caggacaagg cgacgaagcc gcagacagta caaat
//
LOCUS       DAXHNE010000244         1161 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.244, whole genome shotgun sequence.
ACCESSION   DAXHNE010000244 DAXHNE010000000
VERSION     DAXHNE010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.244"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(1..286)
                     /locus_tag="ACFM4Z_002056"
                     /pseudo
     CDS             complement(1..286)
                     /locus_tag="ACFM4Z_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            893..>1161
                     /locus_tag="ACFM4Z_002057"
     CDS             893..>1161
                     /locus_tag="ACFM4Z_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC6022023.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEKTDQKAVSAQQAKE
                     QTSFNNPEPMTGFEHTVTFDFQGTKMVIPYGYLARYTQDNATKWLS"
BASE COUNT          369 a          219 c          227 g          346 t
ORIGIN      
        1 agaatgccga agacaaccac ttttcctgcc gcccgcgacc acgtctgcct ttacgccgtc
       61 cgccgaaatc gctttcgtcc ggctcgacag ggccctcaaa aacctcatcg gcagccaagg
      121 ccaaatgatg gttgataacc gtgcggattt tacggtagaa cagtgctgcc gaattgggat
      181 ggatacccaa aatatcggcg gcagaacggg cggtaatttc cagcgcaaaa aaacggagca
      241 gttctttctg tacttttttc tttaatttgc agtgtgttat cttcatattt cgagggtaac
      301 atatctgcta atctagtaca gccccaaaaa tataccaaaa acagcaaaac aaattgtaag
      361 gataggtata ggctttgtaa aggtaaattg tgaaaaaagc agttttttaa acgaatgaaa
      421 cggcttcggg ctgaaatata tgctgatgcc ctgtccttcc cgtatatctt gtgtgttgtc
      481 aaagtgcagg ctgctttgaa attggcatta cagtcgatga accaccattt tgctttattt
      541 cagcgggctt gagatgtgta taagaatatt gttttgaata aatttaaaga aaatgataat
      601 cgttattgac gatttttaaa ggaaagcgta gagtgccaat tctatgaagc aatacggtaa
      661 gtaacaatga aaatatctac tgcttgggta tagagcatat ttcacaaccc gtaactattc
      721 ttgcggaaac agagaaaaaa gtttctcttc tatcttggat aaatatattt accctcagtt
      781 tagttaagta ttggaattta tacctaagta gtaaaagtta gtaaattatt tttaactaaa
      841 gagttagtat ctaccataat atattcttta actaatttct aagcttgaaa ttatgagacc
      901 atatgctact accatttatc aactttttat tttgtttatt gggagtgttt ttactatgac
      961 ctcatgtgaa cctgtgaatg aaaagacaga tcaaaaagca gtaagtgcgc aacaggctaa
     1021 agaacaaacc agtttcaaca atcccgagcc aatgacagga tttgaacata cggttacatt
     1081 tgattttcag ggcaccaaaa tggttatccc ctatggctat cttgcacggt atacgcaaga
     1141 caatgccaca aaatggcttt c
//
LOCUS       DAXHNE010000245         1131 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.245, whole genome shotgun sequence.
ACCESSION   DAXHNE010000245 DAXHNE010000000
VERSION     DAXHNE010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.245"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(124..552)
                     /locus_tag="ACFM4Z_002058"
     CDS             complement(124..552)
                     /locus_tag="ACFM4Z_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFC6022024.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(533..967)
                     /locus_tag="ACFM4Z_002059"
     CDS             complement(533..967)
                     /locus_tag="ACFM4Z_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFC6022025.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKASGDDGVRKS"
BASE COUNT          302 a          334 c          201 g          294 t
ORIGIN      
        1 atttgaaaac cccattaaac cgtctttaac ccatcgtcct gaacggtaga tgtttcaccc
       61 cctgcgaacc aaaaaaaaga ccgtctgaat acagacggcc aaagcctggt cacacatcac
      121 tgcctaaaat aaagctgcct gctgtgccgc aggtcggctc atttcattga taatcgtata
      181 tcccgttcgt gaagagatgc cgtatttagg gcatagcttc gtcatcgcca taagcccgct
      241 ctccttatca atatcgcgca atttgacaaa ctcctgataa aacctatggt ttctcaactg
      301 tattaacgcc ttaccgcacc gtgggacata caattcctcg ccaccatata cctgcaacag
      361 ctcatgtgtt ttcacttcgc cgatggcttc gaccaaaatt gccaaacgct cggtgtccac
      421 cttgccctta ccaaatttaa accgcgcccc gccaatcgcc ttgaccagct gttccgtcgc
      481 tgccagtccg atgacatcca caatgtccaa cacggtatcc ggcaataaat gttcaacttt
      541 ttcgaacccc atcatcccca ctcgcttttt ccgttttcct gttttccgca atctgcaacg
      601 cagcaaccag tttatgtagc tgcgtatcgt ctaaatattc gaccttatcc ttaccaaaca
      661 tccgccgcgc cattgcgtgt gcatagttcc aatgtttgcc gccgacggtc agcagggctt
      721 cgactttgtc caacattgcc gctgatgatg tccgacgcag atgtggttta ccgtgtgggt
      781 taccttttgc tttaggctta aatccgtgcg accgcatatc agcgacaaca gactcaagtt
      841 cggaaacatc catatccgca cacgaccgct tgcccgtcac acgctccaac accgcgcgat
      901 aggtaccgtc atccaagccc agctcctttt gagcaatctt aattttcgca atcaacgccc
      961 ggcgcattat ctctccaata caacatatag tataaattag tgtatattat accaataaaa
     1021 tacaatatat agtattaaga cgatgttttt gcgaaacgga caggcataaa aaagcaacta
     1081 tatttttcac cccgtcgggc aaaaatacca aaactcaaat caagccgtcc g
//
LOCUS       DAXHNE010000246         1126 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.246, whole genome shotgun sequence.
ACCESSION   DAXHNE010000246 DAXHNE010000000
VERSION     DAXHNE010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.246"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            81..1046
                     /locus_tag="ACFM4Z_002060"
     CDS             81..1046
                     /locus_tag="ACFM4Z_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6022026.1"
                     /translation="MLRLTALTLCTVLALGACSPQNSDSAPQAKEQAVSAAQTESASV
                     TVKTARGDAKVPQNPERIAVYDLGMLDTLNKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGLPAVDEAIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLDNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
BASE COUNT          291 a          322 c          295 g          218 t
ORIGIN      
        1 tatcatttat cctttctaaa gccgttccgg tttgtccgac cgacggcttt gccccaatat
       61 ccccattttg gagacaccct atgttgcgtt tgaccgcatt gaccctctgc accgtcctcg
      121 ccttgggcgc gtgttcgccg caaaattccg actctgcccc acaagccaaa gaacaggcgg
      181 tttccgccgc acaaaccgaa agcgcgtccg ttaccgtcaa aaccgcgcgc ggcgatgcaa
      241 aagtgccgca aaaccccgaa cgcatcgccg tttacgattt gggtatgctc gacaccttga
      301 acaaactggg cgtgaaaacc ggtttgtccg tcgataaaaa ccgcctgccg tatttagagg
      361 aatatttcaa aacgacaaaa cctgccggaa ctttgttcga gccggattac gaaacgctca
      421 atgcctacaa gccgcagctc atcatcatcg gcagccgcgc cgccaaagca tttgacaaat
      481 tgaacgaaat cgcgccgacc atcgaaatga ccgccgatac cgccaacctc aaagaaagtg
      541 ccaaagagcg catcgacgcg ctggcgcaaa tcttcggcaa acaggcggaa gccgacaagc
      601 tgaaggcgga aatcgatgct tcgtttgaag cggcgaaaac tgccgcacaa ggcaaaggca
      661 aaggtttggt gattttagtc aacggcggca agatgtccgc cttcggcccg tcttcacgac
      721 tgggcggctg gctgcacaaa gacatcggtt tgcccgctgt tgacgaagcc atcaaagaag
      781 gcagccacgg tcagcctatc agctttgaat acctgaaaga gaaaaatccc gactggctgt
      841 ttgtcctcga ccgaagcgcg gccatcggcg aagagggtca ggcggcgaaa gacgtgttgg
      901 ataatccgct ggttgccgaa acaaccgctt ggaaaaaagg acaggtggtt taccttgttc
      961 ctgaaactta tctggcggcc ggcggcgcgc aagagctgct gaatgcaagc aaacaggttg
     1021 cggatgcgtt caacgccgcg aaataatgaa acggcggcat tcgatgccgt ctgaaacacg
     1081 gatgcaaacc gcttcccgtg tttcagacgg cattgcccga tacggg
//
LOCUS       DAXHNE010000247         1112 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.247, whole genome shotgun sequence.
ACCESSION   DAXHNE010000247 DAXHNE010000000
VERSION     DAXHNE010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.247"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            153..1052
                     /locus_tag="ACFM4Z_002061"
     CDS             153..1052
                     /locus_tag="ACFM4Z_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6022027.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFGQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFV
                     SGLVSGLMLALSMVFVRKTHEVEPVALFPSMMILSLGGAVSLVVPALLMDGGVLYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQS"
BASE COUNT          184 a          253 c          358 g          317 t
ORIGIN      
        1 gggttttatc cggcaggacg gttttttgcc ccaacggaaa atagccgacc tgcccgtaaa
       61 atcagccgtt tgtccgggtg cagccggggc tttgggcttc agacggcata ttttcggaat
      121 ggcggcattc ttgccgtcgg cgcggcagcc gtatggggaa gggaggggat attgtggtcg
      181 gtaacggcaa aaaatatgcc gcaccattgc tggtgctggg ttgcgtggtg ttcggtctgg
      241 gcagtctgat tgtcagatcc gtccccgtcg gttcgtatgc aatcgcattt tggcggttgc
      301 tgatttcggt gttcgtattt tggtttttag cacggttttt cgggcaaaaa ttcccaaaaa
      361 acaggaaaac cgtccgatat gccctgacgg cgggcgtgtt tctcgctttc gatttggcgt
      421 tgtggcacga aagcatacac gcggtcgggc cgggtatttc caccctgctc aacagcctgc
      481 aaatcttttt cttgtcggca atcggtgttt tctttttcgg cgagcgtttg agcgggctga
      541 aaaaggcagg cttaatatcg gcagttgccg gtgtggcgat gattgccggt gcggaattcg
      601 gctacaacgg taatgcggtt tggggattcg tcagcggttt ggtgtccggg ctgatgcttg
      661 ccctgtcgat ggtgttcgtc cgcaagactc atgaagtcga gccggtggcg cttttccctt
      721 caatgatgat tttgagtttg ggcggcgcgg tatcgctggt tgttccggca ttgctgatgg
      781 atggcggcgt gctttatccg acgacttgga aagatgcggg tttggtgctt gtgtacggcg
      841 tggtgatgca gtgcttcgcg tgggcgatgg ttgcctatgc gattccgctg ctttcgctgt
      901 cgctgacggg gctgctgctt ttgtccgaac cggttgccgc cctgttcatc gattatttcg
      961 ggttgggcaa accgattgaa ggcgtgcagt gggcaggggt ggcgctgacg ctttcggcaa
     1021 tttacctcgg ttcgctgaaa cagcagtctt aatattgatt ccatcaaggc agtattggca
     1081 gttttccgtg caaaacctgc gttaccggac tg
//
LOCUS       DAXHNE010000248         1102 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.248, whole genome shotgun sequence.
ACCESSION   DAXHNE010000248 DAXHNE010000000
VERSION     DAXHNE010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.248"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..963)
                     /gene="mafB"
                     /locus_tag="ACFM4Z_002062"
     CDS             complement(<1..963)
                     /gene="mafB"
                     /locus_tag="ACFM4Z_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC6022028.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGDGRGSVKNRVYAVQTFDATAVSPVLPITHERTGFEGVIGYETHFSGHGHE
                     VHSPFDHHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWENDPNKNW
                     WANRMDDIRGIVQGAANPFLTGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PEAQLAAASLLQDSAFAVKDSINSARQWADAHPNITATAQTALAV"
     gene            complement(1003..>1102)
                     /locus_tag="ACFM4Z_002063"
     CDS             complement(1003..>1102)
                     /locus_tag="ACFM4Z_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC6022029.1"
                     /translation="TGNSAPSVEADNSHEGYGYSDEVVRQHRQGQP"
BASE COUNT          256 a          271 c          256 g          319 t
ORIGIN      
        1 tacggcaagg gcagtttggg ctgttgcagt tatattcgga tgggcatcag cccattgtct
       61 ggcagagttg atactgtctt ttaccgcaaa agcactgtct tgtaataggc tcgcggccgc
      121 aagttgtgct tccggactta aatttcctaa attgtgaata ccctgtaaag ttttccgtgc
      181 tgccgcatag gttaccgggt ttaccgcact gtctgtaatt gccccaactc ccaatccctg
      241 aaaacccgtt agaaaaggat tggctgcacc ttggacgatg ccgcgaatat catccatacg
      301 gttagcccac caatttttat tggggtcgtt ttcccatatc agttttcctg cttcatccgc
      361 acggctgaaa aaaccagagg cggcaccggc attttctttt agccaacggt ctgaaaatgg
      421 ggcttgaggg acaatattag tctttgtttt tgttgaagtt cctttgacat aatagctgta
      481 tatatccctt gctcctccgg ggggcggata atcgctgcct tgcggtccgt catatccatc
      541 ctccggatgg atttccgacc ctgttcgatg aagttggtaa acagtaaaac cgccgtctac
      601 accgccgctg aaatcagaag tgctttttga atcatgatga tcgaacggac tgtgtacttc
      661 atgtccgtgc cctgaaaaat gggtttcata accgataaca ccttcaaacc ctgtccgttc
      721 gtgtgtaata ggcagtacag gactgaccgc agttgcatca aatgtctgga cggcgtaaac
      781 ccgattttta acactgccgc gaccatcgcc gaacagatgg tatttgccac ccggttcgta
      841 gtgttttgcc tgcatatcat cgcgcaaacg ggcatccaaa ccgttcgcat gactaatggg
      901 gatttgcagc aacgatattg ctgctgcaaa cagcatcatg aatttttgaa taggcaaatt
      961 cattttgttt tccttggtag caagcggtta tggtagtgtg aatcaaggtt gcccttgtct
     1021 atgttgtcgc actacttcat cgctgtatcc atacccctca tgactgttat cagcctctac
     1081 ggatggggca gagttacccg ta
//
LOCUS       DAXHNE010000249         1075 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.249, whole genome shotgun sequence.
ACCESSION   DAXHNE010000249 DAXHNE010000000
VERSION     DAXHNE010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.249"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..260)
                     /locus_tag="ACFM4Z_002064"
     CDS             complement(<1..260)
                     /locus_tag="ACFM4Z_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6022030.1"
                     /translation="MCCSLSFAEPARIDDRIIKFRPSKSKFFESTGYRKINNEFSKFT
                     EAANVEHIPTGAKARINAKITASVSRAAVLSGVGKLVRQGAK"
     gene            complement(244..564)
                     /locus_tag="ACFM4Z_002065"
     CDS             complement(244..564)
                     /locus_tag="ACFM4Z_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HFC6022031.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMALIASKEFEEFVRNVKE
                     YDVIECFSYFLNKRVTHNIWKIYFSDESNIFIRKSEENGKISHEFIYSEFSDSNTDFN
                     VLFS"
     gene            complement(683..982)
                     /locus_tag="ACFM4Z_002066"
     CDS             complement(683..982)
                     /locus_tag="ACFM4Z_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6022032.1"
                     /translation="MGYRVGINCFDTRLQADDYLLSSLPPTVTQDGKIIRPERVGDKW
                     ILNGKPVTLSYPKCSNYEQVKSGAYLGSMVLILFVVIYGFRLLINFLKDIGKVGA"
BASE COUNT          327 a          232 c          185 g          331 t
ORIGIN      
        1 aatttcgcgc cttggcggac aagtttgccg actcctgaca agacggcggc gcgggatacg
       61 ctggcggtta tctttgcgtt gattcgggct tttgcgcccg tggggatatg ttcgacattt
      121 gccgcttctg tgaatttaga aaattcatta ttgatttttc tatatcctgt agattcaaaa
      181 aactttgatt tagatggcct aaattttatt attcgatcat ctattcttgc tggttctgca
      241 aaactaagag aacaacacat taaaatcggt gttgctatca gaaaattccg agtaaataaa
      301 ttcatgcgaa atttttccat tttcttctga cttcctaata aaaatattag actcatcaga
      361 aaaataaatt ttccaaatat tatgcgttac cctcttattt aaaaaataag agaaacattc
      421 tattacgtcg tattctttta catttcttac aaactcttca aattccttag acgcaattaa
      481 tgccatcgac tgcccaaaat acttgctgga cggctgatat ttataaagtg ccaactgcgc
      541 ctgcgtgata aacggcttgt tcatgtttct gtctttcaaa ggttgttttg aaagcctgat
      601 tttaaaacac gtcatataaa tatcaaagcg acagacaaag ccaggaaaaa tccgagcaaa
      661 aaccaaaaat cgacaaacat catcacgccc ctactttgcc tatgtctttt aagaaattaa
      721 tcagcagcct gaagccgtaa ataacgacaa acagaattaa aaccatagac ccgagataag
      781 ctccggattt aacttgttcg taattcgaac atttcggata agacagcgtg accggctttc
      841 cgttcaaaat ccatttatcg cccacccttt ccggcctgat gatttttccg tcctgggtaa
      901 cagtaggagg aagggacgac aataaatagt cgtctgcctg caatcttgta tcaaaacaat
      961 ttatgccgac acgatagccc atttatacgc ccctttttct tcactctgtt tatctgacag
     1021 atttaatcat gctccaagcc attttgaagc cttggattgc aagaatcacg gtaat
//
LOCUS       DAXHNE010000250         1056 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.250, whole genome shotgun sequence.
ACCESSION   DAXHNE010000250 DAXHNE010000000
VERSION     DAXHNE010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.250"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..>1056
                     /locus_tag="ACFM4Z_002067"
     CDS             <1..>1056
                     /locus_tag="ACFM4Z_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6022033.1"
                     /translation="GNTLAQQGSYTKTDGTTAKMGDLLLAADNLHSRFKDKVELTAEQ
                     AKAANLAGIGRLRDLREAAALSGDLANMLKAYSAAETKEAQLALLDNLIHKWAETDSN
                     WGKKSPMRLSTDWTQTANEGIALTPSQVAQLKKNALVSLSDKAKAAIDAARDRIAVLD
                     AYTGQDSSTLYYMSEEDALNIVKVTNDTYDHLAKNIYQNLLFQTRLQPYLNQISFKME
                     NDTFTLDFSGLVQAFNHVKETNPQKAFVDLAEMLAYGELRSWYEGRRLMADYVEEAKK
                     AGKFEDYQKVLGQETVALLAKTSGTQADDILQNVGFGHNKNVSLYGNDGNDTLIGGAG
                     NDYLEGGSGSDTYVFGKG"
BASE COUNT          321 a          234 c          248 g          253 t
ORIGIN      
        1 ggtaacactt tggctcagca aggcagctat accaaaacag acggtacaac cgcaaaaatg
       61 ggggatttac ttttagcagc cgacaatctg cacagccgct tcaaagacaa agtggaactc
      121 actgccgaac aggcaaaagc cgccaatctt gcgggcattg gtcgtctgcg cgatttgcgc
      181 gaagctgccg cattgtccgg cgatttggcc aatatgctga aagcttattc tgccgccgaa
      241 actaaagaag cacagttggc attgttagat aatttgattc acaaatgggc ggaaaccgat
      301 tcgaactggg gcaaaaaatc gccaatgcga ctttcaaccg attggacgca aacggctaat
      361 gaaggtattg cactgacacc atcccaagta gcacaactaa aaaagaacgc tttagtttcc
      421 ctttctgata aagctaaagc agctattgac gccgcccgcg accgcattgc cgtgcttgat
      481 gcctacacgg ggcaggattc cagcacactc tattacatga gcgaagaaga cgcgcttaat
      541 atcgtcaaag taaccaacga tacatacgac catctcgcca aaaacatcta ccaaaacctg
      601 ttgttccaaa cccgtttgca gccatatttg aatcaaatca gtttcaaaat ggaaaatgat
      661 acgttcactt tggattttag tggtcttgtt caagcattta accatgtcaa agaaactaat
      721 ccgcaaaaag cttttgtgga tttggccgag atgcttgcat atggcgaact tcgttcttgg
      781 tatgaaggcc gaagactaat ggccgattat gtggaggagg caaaaaaagc aggtaaattt
      841 gaagattacc agaaagtgtt gggtcaggag accgttgcat tattagctaa aacatcgggt
      901 acgcaagcag atgatatcct gcaaaatgta ggctttggtc ataataaaaa tgtttcttta
      961 tatggtaatg acggcaacga cactctgatc ggcggtgccg gtaatgatta cttggagggc
     1021 ggcagcggtt cggatactta tgtcttcggc aaaggc
//
LOCUS       DAXHNE010000251         1041 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.251, whole genome shotgun sequence.
ACCESSION   DAXHNE010000251 DAXHNE010000000
VERSION     DAXHNE010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.251"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..614
                     /locus_tag="ACFM4Z_002068"
     CDS             <1..614
                     /locus_tag="ACFM4Z_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6022034.1"
                     /translation="KDKISTVSDYFRNIRAHSIHPRVSVGYDFGGWRIAADYASYRKW
                     NNNKYSVNTKKVQENQGTRKDLKTENQENGSFHAASSLGLSAIYDFKLNDKFKPYIGA
                     RVAYGHVRHSIDSTKKTTEVVTSTHGGADTKPTIYNGESTQNAYHESHSIRRLGLGVV
                     AGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYRF"
BASE COUNT          290 a          303 c          231 g          217 t
ORIGIN      
        1 acaaagacaa aataagcaca gtaagcgatt atttcagaaa catccgtgcg cattccatcc
       61 accctcgtgt gtcggtcggc tacgacttcg gcggctggag aatagcggca gattatgcca
      121 gttacagaaa atggaacaac aataaatatt ccgtcaacac aaaaaaggtg caagaaaacc
      181 aaggcaccag aaaagacctg aagacggaaa atcaggaaaa cggcagcttc cacgccgctt
      241 cttctctcgg cttatccgcc atttacgatt tcaaactcaa cgataaattc aaaccctata
      301 tcggtgcgcg cgtcgcctac ggacacgtca gacacagcat cgattcgacc aaaaaaacaa
      361 cagaggttgt tacctccacc catggtggtg ctgacacaaa acctacgatt tataatgggg
      421 aaagtacgca aaacgcctat cacgaaagcc acagcatccg ccgcttgggt cttggtgtcg
      481 tcgccggtgt cggtttcgac atcacgccca agctgacttt agacaccgga taccgctacc
      541 acaactgggg acgcttggaa aacacccgct tcaaaaccca cgaagtctca ttgggcatgc
      601 gctaccgctt ctgattcccc gataccgatg ccgtctgaac cttcagacgg cattttttac
      661 acaattccca ccgtttccca tcattcccga tacaccgtaa tctcgaaacc cgtcattccc
      721 gcgcaggcgg gaatccagac ctgtccgcac agaaacttat cggataaaac ggtttcttta
      781 gattttacgt cctagattcc cacttccgtg ggaatgacgg ttcagttgca ttcccgacaa
      841 caacgcaatc tcgaaacccg tcattcccgc gcaggcggga atccagacct ccgacgcggc
      901 gggaatctat cggaaatgac tgaaaccccg agattctaga ttcccacttc cgtgggaatg
      961 acgtggtgca ggtttccgta tggatggatt cgtcattccc gcgcaggcgg gaatccagac
     1021 ccccgacgcg gcgggaatct a
//
LOCUS       DAXHNE010000252         1003 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.252, whole genome shotgun sequence.
ACCESSION   DAXHNE010000252 DAXHNE010000000
VERSION     DAXHNE010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.252"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(137..760)
                     /gene="mtrR"
                     /locus_tag="ACFM4Z_002069"
     CDS             complement(137..760)
                     /gene="mtrR"
                     /locus_tag="ACFM4Z_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HFC6022035.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEGGSWAVFRHTLLHFFERLQSNDIY
                     YKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKETAV
                     IFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT          243 a          215 c          258 g          287 t
ORIGIN      
        1 agattgagca taaaatttta gtaacctatg ttattgcaaa ggtctcaata tgtattttta
       61 gaagaaggct ttgtgtcatt ttgatgccgt ctgaaagggt ttgttcgttt cagacggcat
      121 tgttaccaag gcttgattat ttccggcgca ggtcgggatg gttttccaag ttgtccatca
      181 ttatcccgat gatgcgcggg gcggttttgc ccaaatcgaa acgttcgcag gaagagaacc
      241 accgccaaat cagcccgtcc aaggttgatt tgatgaagat gaccgctgtt tccttgtcca
      301 aatcgtcagc caaatcctga ttttccaccg cttcggtcaa aacggcggta attttctcgc
      361 gccagattgc ctgatgcttg cgggcaatgg cgataacggc ggcgttttgc tccgtgtgtt
      421 cgcattttaa aaacaggatg ttgtggaatt tgtagtagat gtcgttgctt tgcagccgct
      481 cgaaaaagtg cagcagcgtg tggcggaata ccgcccaaga ccctccttcg gcatcttgcg
      541 cgatgcagtt ttcgatgtcg tcgcagatac gttggaacag cgcgtcgaac aagtcttcct
      601 tatttttgaa atgccaatag agcgcgccgc gcgttacgcc ggcggcttgg gcgatttcgt
      661 tgagcgaggt gcgcgcaatc cctttgcggt aaaaggtttc caaggcggca agcatcaggt
      721 gttctttggt tttcaaggct tcggttttgg tttttctcat aatggcgttt tcgtttcggg
      781 tcggtttgat gagggcggat tataaaaaag actttgtaac catgcaatcg tgtatgtata
      841 atgaaaccca tgaaattgag actacatctc aactttgaaa acaggcataa cctgctttgc
      901 aacccgttcg aacattcggg tatcaaagca ggcggttttt atatcctgaa atatagtgga
      961 ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt aca
//
LOCUS       DAXHNE010000253          992 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.253, whole genome shotgun sequence.
ACCESSION   DAXHNE010000253 DAXHNE010000000
VERSION     DAXHNE010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.253"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..227
                     /locus_tag="ACFM4Z_002070"
     CDS             <1..227
                     /locus_tag="ACFM4Z_002070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6022036.1"
                     /translation="YRFLLIRYTTMNPSQYLFIDLNFDKIPDTRLCCFSIFQPTVNLK
                     QPVHAMLQFLFLSADCFDKELKIHFMHLKI"
     gene            335..895
                     /locus_tag="ACFM4Z_002071"
     CDS             335..895
                     /locus_tag="ACFM4Z_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HFC6022037.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINLPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
BASE COUNT          282 a          276 c          201 g          233 t
ORIGIN      
        1 cgtaccggtt tttgttaatc cgctatacca cgatgaatcc ttcgcaatat ctgtttatcg
       61 acctcaattt tgacaaaata ccggatacgc gcctttgttg cttttccatc ttccaaccaa
      121 ctgtaaatct caaacagccg gtacacgcca tgcttcagtt tcttttcctg tcggcggatt
      181 gtttcgacaa agaattgaaa atccatttca tgcaccttaa aatttaatct gcattcaaac
      241 cttttcactt tggaagcacc atttatcgga tgtcccttcg caataaacaa attttcccga
      301 taccgccgcc catttcaacc caaacccaaa acctatgaaa aacctcatcg ccttcaacaa
      361 accctatggc gttatctgcc aattttcacc gcacgaaaaa cacaaaagcc tcaaagactt
      421 tatcaatctt cccggcttct accccgccgg acggctcgac accgacagcg aggggctgct
      481 gctgctgacc gacgacggca ggcttcaggc acaaattacc gaccccaaat tcaaacaccc
      541 caaaacctac tgggcgcaac tggagggcgt acccgacgaa agccgattgg aaagcctaag
      601 aaaagggata gacttaggcg gtttcgttac ccgtccggca agcatccgca tcttgaaaca
      661 cggagaagca gattcgttat gggagcgcat cccgccgata cgcgtccgca aaaccgttcc
      721 cgatttttgg attgaaatta ccatttctga gggcaaaaac cgccaagtca ggcgaatgac
      781 cgccaaggcg ggctatccct gcctgcgtct gatcagagtg gcaagcggca ggctgaaact
      841 gtttgatttg gatttaaaac ccggggaatg ggcatacgcc ccgtttaaac cataatcacg
      901 tttatctcat catttccaca aaagtgggaa tccggaattt tatagtggat taacaaaaat
      961 caggacaagg cggcgaagcc gcagacagta ca
//
LOCUS       DAXHNE010000254          990 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.254, whole genome shotgun sequence.
ACCESSION   DAXHNE010000254 DAXHNE010000000
VERSION     DAXHNE010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.254"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..412
                     /gene="mafB"
                     /locus_tag="ACFM4Z_002072"
     CDS             <1..412
                     /gene="mafB"
                     /locus_tag="ACFM4Z_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC6022038.1"
                     /translation="YETPAVRHMQTMDGEMAGGNRPPKSITSNGKADASTQPSLQAQL
                     IGEQISSGHAYNKHVIRQQEFTDLNINSPADFARHIENIVANPSESKKLSNGRSAYWD
                     DKSGTVVIRNPKDPDGGTAFRPKIGKQYFDDKLK"
     gene            425..634
                     /locus_tag="ACFM4Z_002073"
     CDS             425..634
                     /locus_tag="ACFM4Z_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6022039.1"
                     /translation="MKLIGFEENLVKISLNKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKNKVLSLDKELKKIYDTWDKC"
     gene            743..976
                     /locus_tag="ACFM4Z_002074"
                     /pseudo
     CDS             743..976
                     /locus_tag="ACFM4Z_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          355 a          147 c          207 g          281 t
ORIGIN      
        1 ctatgaaaca cctgctgttc gccatatgca gactatggat ggggagatgg caggtgggaa
       61 tagaccgcct aaatctataa cgtccaacgg caaagcagat gcttccacac aaccgtcttt
      121 acaagcgcaa ctaattggag aacaaattag tagtgggcat gcttataaca agcacgtcat
      181 aagacaacaa gaatttacgg atttaaatat caattcacca gcagattttg ctcggcatat
      241 tgaaaatatt gttgccaatc cttccgaaag caaaaaatta tccaatgggc gtagtgcata
      301 ttgggacgat aagtctggaa cggttgtaat tagaaatccc aaagacccag atggtgggac
      361 agcatttaga ccaaaaatag gtaaacagta ttttgatgac aaattaaagt aaaaggaatt
      421 attcatgaaa ttaattggtt ttgaagagaa tttagttaaa attagtctca ataaagatga
      481 gttgtatatt attcaagcaa ttgtaggaga aatttattca ggcgtatgcg tggattgtag
      541 agattttgag ataattcatg gtgttgaaaa aaataaagtt ctttctttgg ataaagaact
      601 taaaaaaata tatgatactt gggataaatg ttagctaggt agggtgggca ctcgttgccc
      661 accttttgcc gtatattaaa aatccttttc aggctggaac tgagtgggca tcaatgccaa
      721 ccctaattgt gctgatgaag cagtattaag acaagcatcg aatggtgatt atcatggttt
      781 tccgcaatca gtagatgctt tttctgaaaa tggaacagta atacaaatag ttggcggaga
      841 taatattgta aggcataaac tttatatccc tggatcttat aaaggaaaag atggaaattt
      901 tgaatatatc agagaagcag atggaaaaat taatcataga ttatttgtcc ctaaccaaca
      961 acttcctgaa aaatagtaga tagagattaa
//
LOCUS       DAXHNE010000255          982 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.255, whole genome shotgun sequence.
ACCESSION   DAXHNE010000255 DAXHNE010000000
VERSION     DAXHNE010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.255"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(464..>982)
                     /locus_tag="ACFM4Z_002075"
     CDS             complement(464..>982)
                     /locus_tag="ACFM4Z_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6022040.1"
                     /translation="PFTDGNGRTGRILNSLLLIDKGLLDLPILYLSRYIIENRADYYR
                     LLLGVTERQDWESWIIYILDGVADTADWTVSKIDAIRRLFEQTRQHIRTHAQGIYTHE
                     LVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELSDIGVLQEIVIGRDKLFIHPRLME
                     LLRGEGNSFTSF"
BASE COUNT          245 a          213 c          225 g          299 t
ORIGIN      
        1 taggtttcca tgcggacgcg ttcggattca cgactgcgcg gaaatgacgg gattttggtg
       61 tattccctaa aaaaataaaa aaacatttgc aactttgtta aaaataaagg ctgtgtttta
      121 acgatgtgtt gatatttaat tttagaaagg tagctattta atagttacct tttcttattt
      181 aaaaatagct ttctcaaatt ccatgaacgc ctcaatacga tatgcagatg ctctatcgaa
      241 attaagtttc aacattttgt ttattaaaca ttttatttta gccatttttc aatatacccc
      301 caaatatacc cccaatttgc acaagtcaaa attttaggca ggggttttgt tgctcccatg
      361 atacgcgggc ggatgccggc ttttcgcggg ggaaatacaa ggggtctcgg ttcgggtgtc
      421 aaaatccctg tttcgtgtta gtcatgtggg ggggaagagg gggttagaat gaagtaaagc
      481 tgttgccctc tcctcgcaat agttccatta ggcgcggatg aatgaatagt ttgtccctgc
      541 cgatgacgat ttcttgcagc acacctatgt ctgaaagctc tttcaggtac ttagaggccg
      601 tctgccgttt ggctatccct gccgcttcta ggttggcaat gcgtgtatat ggctgctcaa
      661 acagaagatt taccagttcg tgcgtgtaga ttccttgtgc gtgtgtccgt atgtgttgac
      721 gtgtctgctc gaacaggcgg cgtatcgcat ctattttcga taccgtccaa tcggcggtgt
      781 cggctacgcc gtctaagatg tagattatcc agctttccca atcctgccgt tcggttacgc
      841 ctaaaagcag gcggtaatag tccgccctgt tttcgataat atagcggctc aaatacaaaa
      901 taggcaaatc caaaagccct ttatcaatca atagcaggct gttcaatatg cgccccgtcc
      961 gcccgttgcc gtccgtaaac gg
//
LOCUS       DAXHNE010000256          978 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.256, whole genome shotgun sequence.
ACCESSION   DAXHNE010000256 DAXHNE010000000
VERSION     DAXHNE010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.256"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..>978
                     /locus_tag="ACFM4Z_002076"
     CDS             <1..>978
                     /locus_tag="ACFM4Z_002076"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFC6022041.1"
                     /translation="VCVLEKQHFPRFVIGESLLPHCMEMLEEAGFADAVRAEPGFQLK
                     NGAAFSWGSRYTEFDFTDKFSDGPGTTYQVRRAVFDKILIEEAAKQGVEVRFGHGVTA
                     FDNSGDFVRLNIETDTGKRYELTAKFVLDASGYGRVLPRLLNLETPSHLPPRQAHFTH
                     IDDNITNPKFDRNKILITTYPQHRDVWIWLIPFGDNRCSAGVVGTPDKLVGESETVLK
                     KFVYECPMLNEILDKAVWETDFPFHSIQGYSANVKSLHGRHFALLGNAAEFLDPVFSS
                     GVTIALHSARLAADLLAKQLKGEAADWQTEFAEPLMIGVDAFRTYSGLN"
BASE COUNT          230 a          290 c          252 g          206 t
ORIGIN      
        1 aagtctgcgt gttggaaaaa cagcactttc cgcgcttcgt catcggcgaa agcctgctgc
       61 cgcactgtat ggaaatgctg gaagaagccg gatttgccga tgccgtgcgc gccgagcccg
      121 gctttcagtt gaaaaacggc gcggcgtttt cgtggggcag tcgttatacc gaattcgatt
      181 tcaccgataa attttcagac ggccccggca cgacttatca agtacgccgc gccgtgttcg
      241 ataaaatcct gattgaagaa gccgccaaac aaggcgttga agtacgtttc gggcacggcg
      301 taaccgcgtt cgacaacagc ggcgattttg tccgcctgaa catcgaaacc gacaccggca
      361 agcgctatga actgaccgcg aaattcgtct tggacgcaag cggttacgga cgcgtgctgc
      421 cgcgcctgct aaacttggaa acgccctcgc acctgccgcc gcgccaagcg catttcacgc
      481 acatcgacga caacatcacc aacccgaaat tcgaccgcaa caaaatcctg attaccacct
      541 atccgcaaca ccgcgacgtg tggatttggc tgattccctt cggcgacaac cgctgttccg
      601 ccggcgtggt cggcacgccc gacaaacttg tcggcgaatc ggaaacagtg ttgaaaaaat
      661 ttgtttacga atgcccgatg ctgaacgaaa ttttggacaa agccgtttgg gaaaccgatt
      721 ttccgttcca ctccatccaa ggctattccg ccaatgtgaa atcgctgcac ggcaggcatt
      781 tcgcgctatt gggcaatgcc gccgagttcc tcgatcccgt gttctcgtcg ggcgtaacca
      841 tcgcgctgca ctccgccaga cttgccgccg atctgttggc aaaacaactc aaaggcgaag
      901 ccgccgattg gcaaaccgaa tttgccgaac ccttgatgat cggcgtggac gcgttccgca
      961 cctatagtgg attaaatt
//
LOCUS       DAXHNE010000257          949 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.257, whole genome shotgun sequence.
ACCESSION   DAXHNE010000257 DAXHNE010000000
VERSION     DAXHNE010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.257"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..227)
                     /locus_tag="ACFM4Z_002077"
     CDS             complement(<1..227)
                     /locus_tag="ACFM4Z_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6022042.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASVSRAGVLAG"
     gene            complement(211..528)
                     /locus_tag="ACFM4Z_002078"
     CDS             complement(211..528)
                     /locus_tag="ACFM4Z_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HFC6022043.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMAVIAQSEFVEFAKINKS
                     ENVIDCFSFFWNRRIKHDIWLISFSDNSEMVIKESLKDGHKIYKFEFCEIVDNCNFDD
                     VFV"
     gene            complement(656..934)
                     /locus_tag="ACFM4Z_002079"
     CDS             complement(656..934)
                     /locus_tag="ACFM4Z_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6022044.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSPKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
BASE COUNT          273 a          208 c          168 g          300 t
ORIGIN      
        1 cccgccaata cgccggcgcg ggatacgctg gcggttattt tggcgttgat tcgggctttt
       61 gcgcccgtag ggatgtgtgt taaatctacc gtttttatta aatcagatga ataagtttta
      121 ctatttttag gtacaaactt atgaattttc gcaccttgtc cggtatcaac tgaaacagtt
      181 tcagatattt ttactgcatt tgcattcgct tcaaacgaat acatcatcaa aattgcaatt
      241 atcgacaatt tcgcaaaatt caaatttgta tattttatga ccatctttca gagattcttt
      301 aattaccatt tctgaattat cagaaaatga gattagccaa atatcatgtt taattcttct
      361 attccagaaa aaagagaaac aatcaataac attttcagac ttattaatct tcgcaaattc
      421 aacaaattca gattgcgcta taaccgccat cgattgccca aaatacttgc ttgacggctg
      481 atatttataa agtgccaact gcgcctgagt gataaacggc ttgttcatgg ttctgccttt
      541 caaaggttgt tttgaaagcc tgattttgac accataactt catgcgctca attcttaaac
      601 agaaccgccc cgattaatac gggtacggaa acgccgagat aaaaataaaa atccatcatt
      661 tcaaaacctt tttcagcagg gaaacaaagt aaacggacgc gaggatgccg aatactatcc
      721 agcctgtttc aagaccgctt tgcaggttgt ctttcggact gcattccgcc aataaaagcc
      781 ttagcggctg accgtccgac atcttccaca ggctgccgtt atattccggc ctgacaatct
      841 gtccgttttc tttgattctt ggtactacca agctgaaata aaggttttca gcctggtgct
      901 tctcaagaca tttatttccg acttggtagt acatgccgtc ttacttcat
//
LOCUS       DAXHNE010000258          949 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.258, whole genome shotgun sequence.
ACCESSION   DAXHNE010000258 DAXHNE010000000
VERSION     DAXHNE010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.258"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..939
                     /locus_tag="ACFM4Z_002080"
     CDS             <1..939
                     /locus_tag="ACFM4Z_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC6022045.1"
                     /translation="YDYATGRKDIIRFTDGITADMLTFTREGNHLLIKAKDDSGQVTV
                     QSYFQNDGSGAYRIDEIHFDNGKVLDVATVKELVQQSTDGSDRLYAYQSGNTLNGGLG
                     DDYLYGADGNDLLNGDAGNDSIYSGNGNDTLNGGEGNDALYGYNGNDVLNGAEGNDHL
                     NGEDGNDTLIGGAGNDYLEGGSGSDTYVFGKGFGQDTVYNYHVDKNSDTMHFKGFKAA
                     DVHFIRSGSDLVLSASEQDNVRISGFFYGENHRVDTFVFDDAAISNPDFAKYINAGNN
                     LVQSMSVFGSNTAATGGNVDANIQSVQQPLLVTPSA"
BASE COUNT          265 a          202 c          237 g          245 t
ORIGIN      
        1 tacgactacg ctaccggacg caaagacatc atccgcttta ccgacggtat tacagccgat
       61 atgctgactt ttacccgaga gggcaaccat cttcttatca aggcaaaaga cgacagtgga
      121 caagtgactg ttcagtccta tttccagaac gatggctcag gtgcttaccg tatcgacgag
      181 attcatttcg ataacggcaa agtactggat gttgccactg tcaaagaact ggtacagcaa
      241 tccaccgacg gttcggacag attgtatgcc taccaatccg gaaatacctt aaatggcgga
      301 ttgggcgatg actatctgta cggtgccgac gggaatgacc tgctgaatgg tgatgcaggc
      361 aacgacagta tctacagtgg caatggcaat gatacgctca atggaggaga aggcaacgac
      421 gccctgtacg gctataatgg taacgatgta ctgaatggtg ccgaaggcaa tgatcatttg
      481 aacggcgaag acggtaacga cactctaatc ggcggtgccg gtaatgatta cttggagggc
      541 ggcagcggtt cggatactta tgtcttcggc aaaggcttcg gtcaggatac ggtctataat
      601 taccatgtgg ataaaaactc tgacactatg cactttaaag gatttaaagc agcagatgtt
      661 cattttatcc gttccggaag tgatttggtg cttagcgctt ctgaacaaga caacgtacgt
      721 atttccggat tcttctatgg tgaaaaccat cgtgtagata catttgtctt tgatgatgca
      781 gctatcagta atccagattt tgccaagtat attaatgctg gcaataattt ggtacagtct
      841 atgtctgtgt tcggttctaa tactgctgcg acaggaggaa atgtggatgc caatatacaa
      901 tccgtacagc agccgttatt ggtaacgcca tctgcataag gagcctaat
//
LOCUS       DAXHNE010000259          945 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.259, whole genome shotgun sequence.
ACCESSION   DAXHNE010000259 DAXHNE010000000
VERSION     DAXHNE010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.259"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..231
                     /locus_tag="ACFM4Z_002081"
     CDS             <1..231
                     /locus_tag="ACFM4Z_002081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6022046.1"
                     /translation="RLVLIFVNPLYADAGDVYCVYNIRLKQYTACQVTRVEEHGGKKN
                     TCDPAGFGLAGQQTAWGGGAGGFETALQRLKE"
     gene            605..>945
                     /locus_tag="ACFM4Z_002082"
     CDS             605..>945
                     /locus_tag="ACFM4Z_002082"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HFC6022047.1"
                     /translation="MYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDGYDVYRQLR
                     WQQISEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELKAFPCLSSL
                     FLTKWHKNLYD"
BASE COUNT          291 a          156 c          218 g          280 t
ORIGIN      
        1 cgccttgtcc tgatttttgt taatccacta tatgctgatg ccggagacgt atattgcgtc
       61 tataacatca gactgaagca gtacactgcc tgccaggtta cccgagttga agaacacggt
      121 ggcaaaaaaa acacatgcga ccctgctggc tttgggctgg cagggcaaca aaccgcttgg
      181 ggcggaggag ctggcggatt tgaaaccgct ttacaaagac ttaaagaata atattggaaa
      241 tattgtatga acaaaaaatt aaactatatt tttatgttgg actgtttagg gttggtgata
      301 ttgtttactt gtataatagc tacttttgaa agagattatg gatttaaaat ttttactaat
      361 tctaagaggc ctgaatttta ttattggatt ggaatgtttt attatggaat tatttcttgc
      421 tggtttgatt atcaattaat ttcaacaaag gcgaattcgt ataaaagaaa agttaaacaa
      481 tataaaattt tttcagtaat attttcagtt ttgatattta tttctactat agtaaaactt
      541 taaattttgg agcaaaaatt tatgagcgat tcaattgaat atgtattggg aacgcggtct
      601 gcacatgtat aaggcaagtg ccgtcgtgcc gacgggatat gtacgggttg ggaataccgc
      661 gccgctggtc ggcgaagaca cgcaacggta tgcctctttt tggggcgacg gctacgacgt
      721 gtaccgtcag ttgagatggc agcagatatc cgaaaaacag agaaaggcat tcaaaaaagc
      781 cgccaaaagc aaaaagaccg tgatgtttgc cggacgggaa tacggcatat ccaaacaaaa
      841 tttgagcgat gtttgggacg attttgaaga cgcgatggaa ctgaaggcgt ttccctgcct
      901 gtcttcactg tttctgacca aatggcataa aaatctatat gatag
//
LOCUS       DAXHNE010000260          940 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.260, whole genome shotgun sequence.
ACCESSION   DAXHNE010000260 DAXHNE010000000
VERSION     DAXHNE010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.260"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(467..751)
                     /locus_tag="ACFM4Z_002083"
     CDS             complement(467..751)
                     /locus_tag="ACFM4Z_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6022048.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
BASE COUNT          269 a          249 c          170 g          252 t
ORIGIN      
        1 cggactttca gataatcttt gaatattgcc gctgtccaat ggtctagatt cccgcctgcg
       61 cgggaatgac ggtttagaag ttgcccgaaa cctcaaaaaa accgaaaccg aacaagccgg
      121 attcccgcct gcgcgggaat gacggcagaa cggtttcggt tttttccgat aaatgccgca
      181 atctcaaatc ccgtcattcc cgcgaaccta catcccgtca ttcccacgaa agtgggaatc
      241 tagaatctct aaagcttcag ctaacctttg aatattactg ttgttctaag gtctagattc
      301 ccgcctgcgc gggaataacg ggtcttttat aaactttgaa tattgccgtt atcccaaggt
      361 ctagattccc gcctacgcgg gaatgacggt ttagaagttg cccgaaacct caaaaaaaaa
      421 accgaaaccg aacaagccgg attcccgcct gcgcgggaat gacgggctaa ataatatcaa
      481 accataaatc ctgccaagaa acattatttt cttcaatcag ttgcaatttc caagccctgt
      541 tccatttctt caactgtttt tcccgagtaa ttgcactctc catcgtagga tgcagttcat
      601 accaaaccag catagtaacg ttgtaccgtg atgtaaatcc ctcaatcaaa tgctccctat
      661 gttggtaaat acgttgcacc aaatcagatg taacgccaat gtataacgtg ccattacgtt
      721 ggcttgctaa aatataaacc gcaggctgca tataataccc ttttgaatta tttcaattta
      781 tattcccgcg aacaccatcc cgtgattact ttaacccttc gttattccca tagctttcca
      841 tcattcccgc aactcttcgt cattcccgcg aaagtgggaa tctagaacgc aaaatctaaa
      901 gaaaccgttt tacccgataa gtttccgcac cgacaaacct
//
LOCUS       DAXHNE010000261          839 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.261, whole genome shotgun sequence.
ACCESSION   DAXHNE010000261 DAXHNE010000000
VERSION     DAXHNE010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.261"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            21..689
                     /locus_tag="ACFM4Z_002084"
     CDS             21..689
                     /locus_tag="ACFM4Z_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6022049.1"
                     /translation="MQADLAYAAERITHDYPKPTGANNTSTVSDYFRNIRAHSIHPRV
                     SVGYDFGGWRIAADYASYRKWNNNKYSVNIKKGRETQHNREELKTENQENGTFHAASS
                     LGLSAIYDFKLNDKFDKFKPYIGARVAYGHVKHQVHSVETKTTIVTSKPNGGPVPSSV
                     TKPPYHQSHSISSLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLKNTRFKTHEVSLGM
                     RYHF"
BASE COUNT          237 a          245 c          185 g          172 t
ORIGIN      
        1 gcagccgcag cccgtattat gtgcaggcgg atttagccta cgccgccgaa cgcattaccc
       61 acgattatcc gaaaccaacc ggtgcaaaca acacaagcac agtaagcgat tatttcagaa
      121 acatccgtgc gcattccatc caccctcggg tgtcggtcgg ctatgatttc ggcggctgga
      181 ggatagcggc agattatgcc agttacagaa aatggaacaa caataaatat tccgtcaaca
      241 taaaaaaggg gagagaaacc cagcacaaca gggaagaact gaagacggaa aatcaggaaa
      301 acggtacatt ccacgccgct tcttctctcg gcttatccgc catttacgat ttcaaactca
      361 acgataaatt cgataaattc aaaccctata tcggtgcgcg cgtcgcctac gggcacgtta
      421 aacatcaggt tcattcggtg gaaaccaaaa ccacgattgt tacctctaaa ccaaatggag
      481 gccctgtccc aagttcggtt accaaacctc cctatcacca aagccacagt atcagcagct
      541 tgggtcttgg tgtcatcgcc ggtgtcggtt tcgacatcac acccaagctg accttggaca
      601 ccggataccg ctaccacaac tggggacgct tgaaaaacac ccgcttcaaa acccacgaag
      661 tctcattggg catgcgctac cacttctgat tccccgatac cgatgccgtc tgaaccttca
      721 gacggcattt ttgattcacc tgccgttgac aaacgcgggg cgggcgtgga aatacccgaa
      781 ccgtcattcc cgacaacacc gtaatcttga aacccgtcat tcccgcgcag gcgggaatc
//
LOCUS       DAXHNE010000262          767 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.262, whole genome shotgun sequence.
ACCESSION   DAXHNE010000262 DAXHNE010000000
VERSION     DAXHNE010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.262"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(90..641)
                     /locus_tag="ACFM4Z_002085"
     CDS             complement(90..641)
                     /locus_tag="ACFM4Z_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6022050.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
BASE COUNT          218 a          286 c          142 g          121 t
ORIGIN      
        1 caaagaaacc gccattagca ctacaccccc caagctgcgg ctgccaaaca catcggctgc
       61 ttctcgacgc acccgctgaa aaatgcgtcc tatccgtccg ccttgcccgc accccctgac
      121 tttgccgccc tgcccgatgc cgtctgaagc ctgaaccgcc ccaccagccc gatgacgcac
      181 gacaccgccg gaaagggcat acacaaagcc gtcagcacat aacgcgcatt ccccgtattg
      241 accacgtccg cgcccttcag cagcagcggc agcgcgttcg cccactccca caccgacggc
      301 gcgctgaacg ccaataccgc caccagcccc aacatccgga acaccccgat ttccaacacc
      361 tgccgccgcc gcaccgaacg gttgagcaaa aacacaaccg ccaacccgaa acagaccacc
      421 gccgccatac cgccgttggc gacaatctgc aaactgttga atgcaatatc gggcgtaacc
      481 gccaacacgc cttccgaatc cggcacggca cgctgcaaat ccaaaccctg caacacattc
      541 aacacaaaac cgtagatcaa atcaaaaaac accacgccta tcggcaaagc actcaaaacc
      601 cgccacatct tcctgtccga ccaaccgttc aaaactttca ttttacccga ccacgcaagc
      661 cgccgaacaa aaaacaaggg gctgtcctag ataactagga caaacttgat tttactaatt
      721 gttttaaaat ggaacaagaa cttttatctc actgttgtta aaacgcc
//
LOCUS       DAXHNE010000263          767 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.263, whole genome shotgun sequence.
ACCESSION   DAXHNE010000263 DAXHNE010000000
VERSION     DAXHNE010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.263"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..760
                     /locus_tag="ACFM4Z_002086"
     CDS             <1..760
                     /locus_tag="ACFM4Z_002086"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFC6022051.1"
                     /translation="RFIDVNPEDFAGFPLNIRHISITTYARLKLGEYIADCDKVLYLD
                     IDVLVRDSLTPLWDTDLGDNWLGACIDLFVERQEGYKQKIGMADGEYYFNAGVLLINL
                     KKWRRHDIFKMSCEWVEQYKDVMQYQDQDILNGLFKGGVCYANSRFNFMPTNYAFMAN
                     RFASRHTDPLYRDRTNTVMPVAVSHYCGPAKPWHRDCTAWGAERFTELAGSLTTVPEE
                     WRGKLAVPHRMFSTKRMLQRWRRKLSARFLRKIY"
BASE COUNT          189 a          188 c          209 g          181 t
ORIGIN      
        1 ccgctttata gacgtaaacc ccgaagattt cgccggcttc cccttaaaca tcaggcacat
       61 ttccattacg acttatgccc gcctgaaatt gggcgaatac attgccgatt gcgataaagt
      121 cctgtatctg gatatagacg tattggtcag ggacagcctg acgcccttat gggataccga
      181 tttgggcgat aactggcttg gcgcgtgcat tgatttgttt gtcgaaaggc aggaaggcta
      241 caaacaaaaa atcggtatgg cggacggcga atattatttc aatgccggcg tattgctgat
      301 caacctgaaa aagtggcggc ggcacgatat tttcaaaatg tcctgcgaat gggtggaaca
      361 atacaaggac gtgatgcaat atcaggatca ggacattttg aacgggctgt ttaaaggcgg
      421 ggtgtgttat gcgaacagcc gtttcaactt tatgccgacc aattatgcct ttatggcgaa
      481 caggtttgcg tcccgccata ccgacccgct ttaccgcgac cgtaccaata cggtgatgcc
      541 cgtcgccgtc agccattatt gcggcccggc aaagccgtgg cacagggact gcaccgcgtg
      601 gggtgcggaa cgtttcacag agttggcggg cagcctgacg accgttcccg aagaatggcg
      661 cggcaaactt gccgtcccgc accgtatgtt ttcgacaaag cgtatgcttc aaagatggcg
      721 cagaaagctg tctgccagat tcttacgcaa gatttattga cggggca
//
LOCUS       DAXHNE010000264          764 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.264, whole genome shotgun sequence.
ACCESSION   DAXHNE010000264 DAXHNE010000000
VERSION     DAXHNE010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.264"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(486..>764)
                     /locus_tag="ACFM4Z_002087"
     CDS             complement(486..>764)
                     /locus_tag="ACFM4Z_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6022052.1"
                     /translation="ACDRLPESNPAEDLNLPSETVNVEFQKSGIFQDSAQCPAPVTFT
                     VTVLDSSRQFAFSFENACTIAERLRYMLLALAWAVAAFFCIRTVSREV"
BASE COUNT          143 a          232 c          219 g          170 t
ORIGIN      
        1 ttttaccatg gcttccgccc agaccggcgg tacttcgcct gcctttcggc cgtggcttcc
       61 gcctaagcct ggcggtgctt cgccctgcct ttcggccgtg gcttccgccc aaccggcggt
      121 gcttcgcctg cctgtagcct ggcttccgcc tagctcggcg gtgctgcgcc tgcctgtagc
      181 ctggcttccg ctcagaccgg cagtgctgcg cctgcctttc ggccgtggct tccgaagctg
      241 aaaggctttg cggggactag cccccgcacc cccagtctca cttgcgacgc cgcgggggca
      301 gggggaacgg cgcaaaaggc gcgcgcctta ccacctgccc ttgcggcaga atgtgttctt
      361 ttggcggggc ggcaaggggt atccaaaaag atttataaag acgataaagc cgtctttaca
      421 aatctttctg gacgtcctcc ccctgccttg gtacaagtta ctgaagcccg gcggtgctgc
      481 gcctgctaga cttcacgaga tactgtgcgg atacaaaaaa aggcggcaac cgcccaagca
      541 agggcgagaa gcatgtacct tagccgttcg gctatggtac atgcgttctc aaagctgaac
      601 gcgaactgcc tgcttgaatc aagcacagtc actgtgaaag tgacaggtgc gggacactgt
      661 gcggaatctt gaaagattcc tgatttctga aactctacat tgacggtttc agacggcaga
      721 tttaaatctt ctgccggatt ggactcgggc agcctgtcgc aagc
//
LOCUS       DAXHNE010000265          724 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.265, whole genome shotgun sequence.
ACCESSION   DAXHNE010000265 DAXHNE010000000
VERSION     DAXHNE010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.265"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(127..>724)
                     /locus_tag="ACFM4Z_002088"
     CDS             complement(127..>724)
                     /locus_tag="ACFM4Z_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFC6022053.1"
                     /translation="LSIGQIVKVKPAGYAAPKTSAVESRPAVPAAVQTPVKPAAQPPV
                     QSAPQPAAPAAENKAVPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGV
                     DIAGNAGQPVLAAADGKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQV
                     KRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSYIAF"
BASE COUNT          162 a          207 c          198 g          157 t
ORIGIN      
        1 aaacacagtc aaacaaattt atatgcccca tcccttccga atgatttgaa aacacagccg
       61 ccaaaaacaa aaatgccgtc tgaaaacctt tcagacggca tttccaactt gatttcaggc
      121 agaaagtcag aacgcgatat agctgttcgg attaaccggt ttgccgtttt gccgcacctc
      181 gaaatgaagc tgcgttctgg aagcgtcggt attgcccatc aaagcgacct gctgcccgcg
      241 tttgacctgc tggccttcgc cgaccagcaa tttttggttg tgcccgtatg cggtcaggaa
      301 ggaagaatta tgctggatga tgaccagatt gccgtatccc ctcaaaccgg aacctgcata
      361 aaccactttg ccgtcagccg ccgccaaaac gggctgtccc gcattaccgg caatatcgac
      421 acccttgttg ttgccgccga aatcggcaac cactttacct tgcgtcggac gctgccaaac
      481 aatgccgccg accgaacgcg tgccggaagg cgaagcggca ggagattgcg gggcgggcgc
      541 gggaaccgct ttattttccg cagcgggcgc ggcaggttgc ggcgcggact gcacaggcgg
      601 ttgcgcggcg ggtttcacag gggtttgcac ggcagccggt acggcgggcc tgctttctac
      661 ggctgaggtt ttcggtgcgg catatcctgc cggtttgact ttaacaatct gaccgatgct
      721 caac
//
LOCUS       DAXHNE010000266          718 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.266, whole genome shotgun sequence.
ACCESSION   DAXHNE010000266 DAXHNE010000000
VERSION     DAXHNE010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.266"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..>718
                     /locus_tag="ACFM4Z_002089"
     CDS             <1..>718
                     /locus_tag="ACFM4Z_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6022054.1"
                     /translation="TRLEKFDRVALQHFSQYVDLINENNGRLPNTSEIERSYYKAVTE
                     NGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNAQAVNNPNGSEWDNRKQLIS
                     ALDKGFDGSFKEKHFTFLQSVIMDVTKLGVEYTIDGWQKIGGWGNGIINDLYKSVAKR
                     EWTGIFEIVNNNIKQFRDLFPNPEGWIDDGHQCFAPWVKETKKRNGKYHVYDPLALDL
                     DGDGIETVATKGFAGSLFDHT"
BASE COUNT          239 a          110 c          163 g          206 t
ORIGIN      
        1 acccgtttag agaaatttga tcgggttgca ttgcaacatt tcagccaata tgtagatcta
       61 attaatgaaa ataatggtag attacctaac actagtgaaa ttgagagaag ttactataaa
      121 gccgttaccg aaaatggtgt ttcttctagt gcagctattg atttagttat taatcgctca
      181 cttccggata tggcagatgg ttattgggca ttaggtttgg ggatagaagc cgaacgtatc
      241 cacaatgcgc aagcagtaaa taatccgaac ggtagcgaat gggataatag aaagcagtta
      301 atatctgctt tagataaagg atttgatgga tcttttaaag agaagcattt tactttttta
      361 caatctgtga taatggatgt aacaaagtta ggtgttgaat atacaataga tggttggcaa
      421 aaaattggag gttggggtaa tgggataatc aatgatttat ataaaagtgt tgcaaaaaga
      481 gagtggactg gaatatttga gatcgttaat aataacatca agcaatttag agatctgttc
      541 ccaaatccgg aaggctggat cgatgatggt caccaatgtt tcgctccttg ggttaaagaa
      601 actaaaaaac gcaatggcaa atatcatgtc tacgaccccc tcgccctaga tttggacgga
      661 gacggtatag aaaccgttgc caccaaaggc tttgcaggca gcttatttga tcacacca
//
LOCUS       DAXHNE010000267          715 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.267, whole genome shotgun sequence.
ACCESSION   DAXHNE010000267 DAXHNE010000000
VERSION     DAXHNE010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.267"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            105..>715
                     /locus_tag="ACFM4Z_002090"
     CDS             105..>715
                     /locus_tag="ACFM4Z_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6022055.1"
                     /translation="MSTFFQQTAQAMIAKHINRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDDTLSELLKLINRQLTEKGLKVEKASAAVIDATIIQTADGKQRQAIE
                     VDEEGQVSGQTTPSKDSDARWIKKNGLYKLGYKQHTRTDAEGY"
BASE COUNT          203 a          221 c          152 g          139 t
ORIGIN      
        1 cgtcattccc gcgcaggcgg gaatctaggt ttgtcggtgc ggaaacttgt tgaaaacttt
       61 gcaaaatccc ctaaattccc accaagacat ttaggagatt ttccatgagc accttcttcc
      121 agcaaaccgc acaagccatg attgccaaac acatcaaccg cttcccgcta ttgaagttgg
      181 atcaagtgat tgattggcag ccgatcgaac aatacctgaa ccgtcaaaaa acccgttacc
      241 ttcgagacca ccgcggccgt cccgcctatc cgctgctgtc catgttcaaa gccgtcctgc
      301 tcggacaatg gcacagcctc tccgatcccg aactcgaaca cagcctcatt acccgcatcg
      361 acttcaacct gttttgccgt ttcgacgaac tgagcatccc cgattacagc accttatgcc
      421 gctaccgcaa ctggctggcg caagacgaca ccctgtccga attgctcaaa ctgatcaacc
      481 gccaactgac cgaaaaaggt ttaaaagtag agaaagcatc cgccgccgtc attgacgcca
      541 ccattattca gaccgccgac ggcaaacagc gtcaggccat agaagtcgat gaagaaggac
      601 aagtcagcgg ccaaaccaca ccgagtaagg acagcgatgc gcgttggatc aagaaaaacg
      661 gcctctacaa actcggttac aaacaacata cccgcaccga tgcggaaggc tatat
//
LOCUS       DAXHNE010000268          710 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.268, whole genome shotgun sequence.
ACCESSION   DAXHNE010000268 DAXHNE010000000
VERSION     DAXHNE010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.268"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..464
                     /locus_tag="ACFM4Z_002091"
     CDS             <1..464
                     /locus_tag="ACFM4Z_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6022056.1"
                     /translation="IAHFDRLIDELDKQIDDHTHTHFDGKAQVAEQIKGIGSITTATL
                     MAMLPELGRLSHKRIASLAGIAPHPRESGETKFKSRCFGGKSAVRKALYMATVAATRF
                     KPLIRDFYQRLLSEGKPYKVAVTACMRKLLTISNARMRDYFAENDTAENGI"
BASE COUNT          173 a          164 c          204 g          169 t
ORIGIN      
        1 tgattgccca ttttgaccgg ctgattgacg aattggacaa acaaatcgac gaccacaccc
       61 acacgcattt tgacggcaaa gcccaagtgg cggagcaaat caaaggcatc ggttcgataa
      121 cgacggctac gctgatggcg atgctgcccg aattggggcg gctgtcgcac aaacggatag
      181 cgagtttggc cggcattgcc ccgcacccga gggagagcgg ggaaaccaaa ttcaaaagcc
      241 gctgctttgg cggaaagtct gcggtacgta aggcgctgta tatggctacc gtggcagcga
      301 cacgttttaa accgcttatt cgggatttct accaacgcct gctgtccgag ggtaagccgt
      361 ataaggttgc cgttacggca tgtatgcgca aactgctgac gatatcgaat gcccggatgc
      421 gtgattattt tgccgaaaac gataccgccg aaaacggtat ctaaacggct tgatttgagt
      481 tttggtattt ttgcccgacg gggtgaaaaa tacagttgct acggctcgat gaatcgtcag
      541 aaatacctgc atcgtcattc ccgcgcaggt gggaatccag accggtcggt gcggaaactt
      601 atcaggtaaa acggtttctt gagatttttc gtcttggatt cccactttcg tgtgaatgac
      661 ggaatgtagg ttcgtgggaa tgacgtggtg caggtttccg tatggatgga
//
LOCUS       DAXHNE010000269          699 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.269, whole genome shotgun sequence.
ACCESSION   DAXHNE010000269 DAXHNE010000000
VERSION     DAXHNE010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.269"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            347..604
                     /locus_tag="ACFM4Z_002092"
     CDS             347..604
                     /locus_tag="ACFM4Z_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6022057.1"
                     /translation="MTTQMGASRDVSKSAKGWSVGIGLNVGKQLTDSVGLEFDPYYRH
                     KTIYKPREIVLDGDKTKMGRSKSNEYGFRVAATFYSQLKSK"
     gene            complement(586..>699)
                     /locus_tag="ACFM4Z_002093"
     CDS             complement(586..>699)
                     /locus_tag="ACFM4Z_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFC6022058.1"
                     /translation="ELNPIEKVWANIKRYLRTVLSDYARFDDALLSYFDFN"
BASE COUNT          214 a          169 c          159 g          157 t
ORIGIN      
        1 gaaaatgttg aaaacaaaaa gaactcattg cttgaaaaaa gatttaatgg gaatgcgggt
       61 atttcccaca ctttttccat ttatttatcc tcattatagg aagaaaaaca tgaaaaaacc
      121 ctgcttgcac tctcacttgc agccgtgtcc gccgttacgg tagccgatat tcccgagtct
      181 aacggcaaag gcaaagccat catcggcgat aaggaaaaac accgttacca agcatgaaaa
      241 ccttgctttg aaagtccatg gcgaaatccg ccaagatttg ggggacggat gatccctgac
      301 cccttgggcg gatgcatatg cagatttgcg cggcaaaacc aaagtgatga cgacccagat
      361 gggtgcttcc cgcgatgtca gcaaaagcgc caaaggttgg agtgtcggta tcggtctgaa
      421 tgtaggcaaa cagttgaccg acagcgtcgg tctcgagttt gatccatact accgtcacaa
      481 aacaatctac aaaccccgtg agattgtctt ggacggtgac aaaaccaaaa tgggccgctc
      541 caaatccaac gagtacggct tccgcgtagc cgcaacgttc tatagtcaat taaaatcaaa
      601 ataggacagt agtgcatcgt caaatcgggc gtaatcagac aaaacggttc gcagataccg
      661 cttaatattc gcccacactt tctcaatcgg gttgagctc
//
LOCUS       DAXHNE010000270          696 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.270, whole genome shotgun sequence.
ACCESSION   DAXHNE010000270 DAXHNE010000000
VERSION     DAXHNE010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.270"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(126..671)
                     /locus_tag="ACFM4Z_002094"
     CDS             complement(126..671)
                     /locus_tag="ACFM4Z_002094"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC6022059.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMHNIVASILRNNY
                     GLTVRTDGEGKGNMPLREAVKLIRGSDVAIEFHTNAAASKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAAKV"
BASE COUNT          155 a          200 c          165 g          176 t
ORIGIN      
        1 cacgcccgac ttacccgggc gcatagacgg atgttgtttc aaatagccaa tattggcagg
       61 cttaatctca aattcaggca tctgcggttt caagacagac agagccaaag caatcaaaga
      121 ctttttcata ccttcgccgc ccccaattcc atcgcaatcg cgtccgcaat cgcgcggcag
      181 atgccccatt tagtagtctt aaacaaggct aaatcagtgt cgttgctgat aaaaaacggc
      241 tcaaacacaa tgccgccggc ttgtgcataa gctaggcgcg aatgctggcc cgcattgtcg
      301 ggtttaaagc cgtcttcgcc gcgcagtttc cagccggttt tcttggcaac agctttgctc
      361 aacacctgac accagcgttt gtttttaacg gtactcaacg cctcaatgcc cgtagctgct
      421 ttactggccg ccgcgttggt atggaactca atcgccacat ccgagccgcg aatcagtttt
      481 acagcttcgc gaagcggcat attgcctttg ccttcgccgt cggtgcgtac ggtcaggccg
      541 taattgttac gcaggattga agccacaatg ttgtgcatat cctgcgccaa gtccgcctca
      601 cggtcgctgc cgttgaccgc acccgggtcg gtgttgctgt gtccagcggt taaggttacg
      661 gttttgccca ttcatcatct ccaaaaaata ttgatt
//
LOCUS       DAXHNE010000271          696 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.271, whole genome shotgun sequence.
ACCESSION   DAXHNE010000271 DAXHNE010000000
VERSION     DAXHNE010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.271"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..306
                     /locus_tag="ACFM4Z_002095"
     CDS             <1..306
                     /locus_tag="ACFM4Z_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HFC6022060.1"
                     /translation="DKKTARMAAKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDQEI
                     APRDLIVYDPLPADEYQDIFDFMKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
BASE COUNT          198 a          171 c          147 g          180 t
ORIGIN      
        1 gataaaaaaa ccgcgcgtat ggctgccaaa gaaacattgt cctatttcga tttctacctg
       61 cagccgcaaa tcgttaccta cgccaaaatc gcatccatgc acggtttcga tttgggcata
      121 gatcaagaaa tcgcaccgag ggatttgatt gtttacgatc cgctgccggc agacgaatat
      181 caagacatct tcgattttat gaaacagtat gacttgtctt acccgtatga atatctgcag
      241 gattggatag attactatac gttcaaaacc gataagctgg tatttggtaa cgcgaagcga
      301 gagtgagccg taaaactctg agctcctgtt ttatagatta caactttagg ccgtcttaaa
      361 gctgaaagat tttcgaaagc tataaattga agcccttcca tagtacatag atctgtgttg
      421 tggcgaggct ttaccacgct gattgccgga gaagaactca acctgctggc aaaacaaggc
      481 atgagatctt tgcaataaca tgagttgaga cctttgcaaa aaagcccttc cccgacatcc
      541 gaaacccaaa cacaggattt cggctgcttt cgtaccaaat acctcctaat tttacccaaa
      601 tatcccctta atcctccccg gatacccgat aatcaggcat ccgggctgcc ttttaggcgg
      661 cgcgggcgca cttagcctgt tggcggcctt caacag
//
LOCUS       DAXHNE010000272          680 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.272, whole genome shotgun sequence.
ACCESSION   DAXHNE010000272 DAXHNE010000000
VERSION     DAXHNE010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.272"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(80..565)
                     /locus_tag="ACFM4Z_002096"
     CDS             complement(80..565)
                     /locus_tag="ACFM4Z_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFC6022061.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          200 a          216 c          120 g          144 t
ORIGIN      
        1 gtactggttt ttgttaatcc actatatttt caaaccggaa aaatgccgtc tgaatcttca
       61 gacggcattc cattcatatt tattcgtctt ttttgtgttc gatggcgttt cgggaacgat
      121 tgccgccgta aggctcttca accgtatatt ccccctcgat aatatcgtca tcgcgggaaa
      181 agccctcttt tctgcccgat tggttcatgt tgaaaaaatt ttccgcacct cctgcctgca
      241 acactgcacc tcccttaaac ggcagcagca gcaacaccgc caacaccgag gatacgaatc
      301 ccggactcat cagacacacc gccgccaccg tataacggat aggccacaac atctgataaa
      361 cggataccct cccgccgctt ctcattgccg cgcccgccaa taaaagaccg gacagccccg
      421 tatgcctgag catcagcaca ccggcggcaa aacctgccgc catcaaaaac aacgtccagc
      481 cgccgcccaa ccaatcggca acccacacaa tcgacataat ctccaaaaac agcagcacca
      541 aaaaaccgat accgaaaaat ctcattgacc gtcatcctta tatttaagta aacagcaaac
      601 cgcccgaaca ggactccaag cgacctgcct gtaaatgatt acaaaaccat gtgcttcaag
      661 ccgaaacaat gtgaaatcac
//
LOCUS       DAXHNE010000273          678 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.273, whole genome shotgun sequence.
ACCESSION   DAXHNE010000273 DAXHNE010000000
VERSION     DAXHNE010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.273"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..90)
                     /locus_tag="ACFM4Z_002097"
     CDS             complement(<1..90)
                     /locus_tag="ACFM4Z_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6022062.1"
                     /translation="MLPSKYEDNALQIKEKSTERTAPFFCTRSY"
BASE COUNT          176 a          176 c          117 g          209 t
ORIGIN      
        1 gtaacttcga gtacaaaaaa acggagcagt tctttctgta cttttttctt taatttgcag
       61 tgcgttatct tcatatttcg agggtaacat atctgctaat ctagtacagc ccctagttta
      121 aagctttgtt tcttaagtcc gcagagtatg ccatggttag accttcaaag ttgagtattg
      181 tactattttg tttttaatta actataaaac gcttgtgtga ataggaagac tgcagaatat
      241 tgctgccttt tcctgtttgc tttaaataaa cagaaaatgc cgtctgaaac cgattttcag
      301 acggcatttg tcccttaaaa cacgtttttt caagcgcgac cataccaaac acccacaggt
      361 tgccttccac ccttcgtaca acctttgctc catcaaacat ccacttccta aatactgatg
      421 tctgcttagg ttgaatcggc attcgtacga ttacggcaca ccgcctaatc tgtacctgcg
      481 tctgttgcgc ccaataccaa aggctacctg aaaatctccc aaacttttca ggtagcctta
      541 tcacgttttc gactgtcacc acacgcccaa agaccgcctg aaaccctttt tcagacgttc
      601 cgctccgata cccccaacaa aacgccgccc gaacgcatgt tcagacggca ttttctttgc
      661 gtcgattcat tgcggtgt
//
LOCUS       DAXHNE010000274          672 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.274, whole genome shotgun sequence.
ACCESSION   DAXHNE010000274 DAXHNE010000000
VERSION     DAXHNE010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.274"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            443..>672
                     /locus_tag="ACFM4Z_002098"
     CDS             443..>672
                     /locus_tag="ACFM4Z_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HFC6022063.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTT"
BASE COUNT          207 a          135 c          132 g          198 t
ORIGIN      
        1 cgggatttga ggtttctgtt tttgattttc tgttttcgta ggaatgacgg tttaaaagtt
       61 acccgaaacc tcaaaaaaaa aggctgtgtt ttaatatagt gttgatagac gtacttggct
      121 tccatgtatt caatcgtgga aatctatatc ttcgtcctcg ccgaaatagt ctatgcccga
      181 tatacaattt tgatacacaa acttggaaat atgggtatcg tcgccggagc gatagaatgc
      241 ggacagttat atatatatgg tttttttagg gaagcgggca gatgaagggc ggattttgaa
      301 ttaaccccat gcaaattgac ttttgcgggc ggttttacct cccatatatt tacaaaagcc
      361 aaatttttaa acatatatcc ttgatatata cacggcgtaa atatatactg gaaacatctt
      421 taaatttttt gaaattttaa atatgagcaa ctggaaaccc gatattccct ataacgattt
      481 accacccctg ccgccaaaac aggatattga aagcaaaacc atcctgaaac gttgtatagc
      541 cgcccgtgca tcccttgccc gtttaaagca ggcggcagaa ctgataccga atcaagccat
      601 gctgattaac acccttcctg ttatggaagc ccgtgcaagt tcagaaattg aaaacatcgt
      661 aaccacaacg ga
//
LOCUS       DAXHNE010000275          658 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.275, whole genome shotgun sequence.
ACCESSION   DAXHNE010000275 DAXHNE010000000
VERSION     DAXHNE010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.275"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..137
                     /locus_tag="ACFM4Z_002099"
     CDS             <1..137
                     /locus_tag="ACFM4Z_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC6022064.1"
                     /translation="RLMVDFSDITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG
                     "
     gene            141..>658
                     /gene="mafB"
                     /locus_tag="ACFM4Z_002100"
     CDS             141..>658
                     /gene="mafB"
                     /locus_tag="ACFM4Z_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC6022065.1"
                     /translation="MKPLRRLTNLLAACAVAAAAFGQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASEEKGNVDEGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTAR"
BASE COUNT          181 a          243 c          151 g           83 t
ORIGIN      
        1 accgcctgat ggtcgatttc tccgacatta ccccctacgg cgacacaacc gcccaaaacc
       61 gtcccgactt caaacaaaac aacggtaaaa accccgatgt cggcaacgaa gtcatccgcc
      121 gccgcaaagg aggataaacc gtgaaaccgc tgcgcagact gacaaacctc cttgccgcct
      181 gcgccgtagc ggccgccgcc ttcggacagc ccgccctcgc agcggacttg gcgcaagacc
      241 cgttcattac cgataacgcc caacggcagc actacgaacc cggcggcaaa taccacctct
      301 tcggcgaccc gcgcggcagc gtttccgacc gcaccggcaa aatcaacgtc atccaagact
      361 atacccacca gatgggcaac ctgctcatcc aacaggcaaa catcaacggc acaatcggct
      421 accacacccg cttttccgga cacggacacg aagaacacgc ccccttcgac aaccacgccg
      481 ccgacagcgc gagcgaagaa aaaggcaacg ttgacgaagg ctttaccgta taccggctca
      541 actgggaagg acacgaacat catcccgccg atgcctacga cggcccgaag ggcggcaatt
      601 accccaaacc tacgggcgca cgagacgaat acacctatca cgtcaacggc acagcccg
//
LOCUS       DAXHNE010000276          622 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.276, whole genome shotgun sequence.
ACCESSION   DAXHNE010000276 DAXHNE010000000
VERSION     DAXHNE010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.276"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
BASE COUNT          125 a          181 c          180 g          136 t
ORIGIN      
        1 ggtatgggaa aacatcaaga tggcggcata aagcgcggta tggcggaaaa cccgcctttt
       61 taaagtttta ttcatcgtat ttccttttcg gtcaaacccc ccgcccttcg ggcatccgtc
      121 cttcggggcg gcaggatcag gctttgtccg ggaggggtgc aagcccttcc gaatcagggc
      181 ggcgccttat gtgccgccct gcgtgttgga acatatttta tcccttaaat cttgcttcgc
      241 gggataccgt cggcggcagg cacgccgcaa acggcgggat tcggcaaacc gcccgattcc
      301 gcgcccgccg aattgctggt tggaaaaccc ctttggcccg gctgccaaag ggggatgttc
      361 ggaaaagtaa cacgcccctt tggccggatg caatgtatag ggttaccgca ttattctacc
      421 cttgcgcgct gatcatggta aagcggtttt cggcaatccg caaccgcctc cttggcccgc
      481 cattgccgcg aaagcgggaa tccggaacgt aaaatctcaa aaaaaccgtt ttatccggca
      541 agtttgcccg ccggcaggcc cgccattgcc gcctgcgcgg caatggcggg ccggggaggc
      601 ggcatttttc aagttgcggc aa
//
LOCUS       DAXHNE010000277          617 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.277, whole genome shotgun sequence.
ACCESSION   DAXHNE010000277 DAXHNE010000000
VERSION     DAXHNE010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.277"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..>617
                     /gene="pilC"
                     /locus_tag="ACFM4Z_002101"
     CDS             <1..>617
                     /gene="pilC"
                     /locus_tag="ACFM4Z_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC6022066.1"
                     /translation="ISQLKDKRVVIFGTGSDLSEEDVDNMEEQYIYGIFDDDTVANNV
                     NVKLSGLGGGLLEQELKQEDKTLFLTDYKRSDGSGSKGWVVKLKDGQRVTVKPTVVLR
                     TAFVTIHKYTGNDKCGAETAILGINTADGGKLTKKSARPIVPEANTAVAQYSGHKQTA
                     NGKSIPIGCMEKDGGTVCPNGYVYDKPVNVRYLDEKKTDGFSTTA"
BASE COUNT          187 a          138 c          170 g          122 t
ORIGIN      
        1 atttcccaac tgaaagacaa acgcgtggtt atcttcggta cgggcagtga tttgagtgaa
       61 gaggatgtgg acaatatgga agaacaatat atttacggta tcttcgacga cgatacggtg
      121 gcgaataacg taaatgtaaa actcagcggt ttgggaggcg ggctgctcga gcaagagctt
      181 aagcaggagg ataaaacctt attcctgacc gattacaagc gatccgacgg atcgggcagc
      241 aaaggctggg tagtgaaatt gaaggacgga cagcgcgtta ccgtcaaacc gaccgtggta
      301 ttgcgtaccg cctttgtaac catccataaa tatacgggta atgacaaatg cggcgcggaa
      361 accgccattt tgggcatcaa taccgccgac ggcggcaagc tgaccaagaa aagcgcgcgc
      421 ccgattgtgc cggaagccaa tacggctgtc gcgcaatatt ccggccataa gcaaaccgcc
      481 aacggcaaat ccatccctat aggttgtatg gaaaaagacg ggggaaccgt ctgcccgaac
      541 ggatatgttt acgacaaacc ggttaatgtg cgttatctgg atgaaaagaa aacagacgga
      601 ttttcaacaa cggcaga
//
LOCUS       DAXHNE010000278          611 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.278, whole genome shotgun sequence.
ACCESSION   DAXHNE010000278 DAXHNE010000000
VERSION     DAXHNE010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.278"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..>611
                     /locus_tag="ACFM4Z_002102"
     CDS             <1..>611
                     /locus_tag="ACFM4Z_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFC6022067.1"
                     /translation="LKIEKASAAVVDATIIQTAGSKQRQAIEVDEEGQINGQTTPSKD
                     KDARWIKKNGLYKLGYKQHTRTDAEGYIEKLHITPANAHECKHLSPLLEGLPKGTTVY
                     ADKGYDSAENRQHLEERQLLDGIMRKACRNRPLTEAQTKRNRYLSKTRYVVEQSFGTL
                     HRKFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
BASE COUNT          180 a          171 c          155 g          105 t
ORIGIN      
        1 ttaaaaatag agaaagcatc cgccgctgtc gttgacgcca ccattattca gaccgccggc
       61 agcaaacagc gccaggccat agaagtcgat gaagaaggac aaatcaacgg ccaaaccaca
      121 ccgagtaaag acaaagatgc ccgctggata aagaaaaacg gcctctacaa actcggttac
      181 aaacaacata cccgtaccga tgcggaaggc tatatcgaga aactgcacat cacccccgcc
      241 aatgcccatg agtgcaaaca cctgtcgccg ttgttggaag gtctgcccaa aggtacgacc
      301 gtctatgccg ataaaggcta cgacagtgcg gaaaaccggc aacatctgga agagcgtcag
      361 ttgttggacg gcattatgcg caaagcctgc cgcaaccgtc cgctgacgga agcgcaaacc
      421 aaacgcaacc ggtatttgtc gaagacccgt tatgtggtcg aacagagctt cggtacgctg
      481 caccgtaaat tccgctatgc gcgggcagcc tatttcggac tgattaaagt gagtgcgcaa
      541 agccacctga aggcgatgtg tttgaacctg ttgaaagccg ccaacaggct aagtgcgccc
      601 gctgccgcct a
//
LOCUS       DAXHNE010000279          578 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.279, whole genome shotgun sequence.
ACCESSION   DAXHNE010000279 DAXHNE010000000
VERSION     DAXHNE010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.279"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..>578
                     /locus_tag="ACFM4Z_002103"
     CDS             <1..>578
                     /locus_tag="ACFM4Z_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISNgo3 family transposase"
                     /protein_id="HFC6022068.1"
                     /translation="KSRFNRTKTDKADSNLIADYIKRHQDTLIPYQIPKNKALQKLIN
                     LKNQLHQHQKQIKNRLHSTEEDFIRNIHQDLIDTIQDKMEQVKIAISEQIKKQTDYNH
                     YRNLQTIPSIGKDTASVLYAQLTEKHFKTANQFVSYAGLSPAIIQSGTSVRGRGRLSR
                     YGNRRLKSTLYMPALCAYRFNAFPKLINNLKKA"
BASE COUNT          236 a          130 c           94 g          118 t
ORIGIN      
        1 aaaagcaggt ttaaccgtac caaaaccgac aaagcagatt caaacctgat agcagattac
       61 ataaaaaggc atcaagatac attgataccg tatcagatac ccaaaaacaa agcactgcaa
      121 aaactgatta accttaaaaa tcaattacat caacatcaga agcaaattaa aaaccgtctt
      181 catagcactg aagaagactt cataaggaac atacatcaag acttgataga taccatacag
      241 gacaagatgg aacaggtaaa aatagccata tccgaacaaa tcaaaaaaca aacggactat
      301 aaccattacc gcaatcttca aaccatcccg agcataggca aagacaccgc atcagttctt
      361 tatgcgcaac tgacagaaaa acattttaaa accgcaaacc agtttgtatc ctatgccgga
      421 ttaagtcccg ccatcataca atcagggaca agcgtaagag gtcggggcag attgagccga
      481 tacggaaaca gacgattaaa aagtacgctg tatatgcccg ccctttgtgc ttaccgtttt
      541 aacgcatttc cgaaattaat aaataatctg aaaaaagc
//
LOCUS       DAXHNE010000280          564 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.280, whole genome shotgun sequence.
ACCESSION   DAXHNE010000280 DAXHNE010000000
VERSION     DAXHNE010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.280"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
BASE COUNT          153 a          159 c          117 g          135 t
ORIGIN      
        1 tgaaaacggg aatctagaac ctcaaacttt cggataatct ttgaatattg ccgtcgccca
       61 aaggcctgga ttcccgcctg cgcgggaatg acggcggagg gtggacgatg ccgtctgaaa
      121 cttcgccatt cccatgatta ccgcaacctt tcgtcattcc cgccaccttt cgtcattccc
      181 gtgaaaacgg gaatctagaa cctcaaactt tcggataatc tttgaatatt gccgctgccc
      241 gaaggtctgg attcccacaa cctttcgtca ttcccgtgaa aacgggaatc tagaacctca
      301 aactttcaga taacctttga atattgccgc tgcccgaagg tctggattcc cacaacattt
      361 cgtcatttcc gtgaaaacgg gaatctagaa cctctaaact ttcagataat ctttgaatat
      421 tgccgtcgcc caaaggcctg gattcccgcc tgcgcgggaa tgacggttta gaagttgccc
      481 gaaacctcaa aaaaaaccga aaccgaacaa gccggattcc cgcaacattc cgtcattccc
      541 gtgaaaacgg gaatctagaa cctc
//
LOCUS       DAXHNE010000281          548 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.281, whole genome shotgun sequence.
ACCESSION   DAXHNE010000281 DAXHNE010000000
VERSION     DAXHNE010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.281"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..>548
                     /locus_tag="ACFM4Z_002104"
     CDS             <1..>548
                     /locus_tag="ACFM4Z_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm49 family immunity protein"
                     /protein_id="HFC6022069.1"
                     /translation="GNTANFNSGCPESIDYVDEKKGVPLAAMKYILMYTEAAASHAYL
                     FEHDLKKFKQYAYVAGKLGILQSVDDEDPEPFFFPCDMLNIQDPMFLMLMSDSPQLRE
                     FLVRNIDNIANDTEAFVNRYDLNRHMIYNTLLMVEGKQLDRLKQRSEKVLAHPTPSKW
                     LQKRLYDYRFFLAFAEQDAEAM"
BASE COUNT          152 a          128 c          127 g          141 t
ORIGIN      
        1 ggcaatactg ctaatttcaa tagcggttgc cccgaatcta tagattatgt agatgaaaaa
       61 aaaggcgtgc cgcttgcagc gatgaaatac attttaatgt acactgaagc tgcggcttcc
      121 catgcctatc tatttgaaca tgatcttaag aaattcaagc aatatgctta tgttgcagga
      181 aagttgggta ttttgcagag tgtagatgat gaagaccccg aacccttctt ctttccctgc
      241 gacatgctca acattcaaga tccgatgttt ctgatgctga tgagcgacag cccgcagctg
      301 cgcgagtttt tggtgcgcaa tatcgacaac atcgccaacg atacagaagc cttcgtaaac
      361 cgatacgacc tcaaccgtca tatgatttac aatactctgc tgatggtgga gggtaagcag
      421 cttgatcggt tgaaacaacg tagcgagaaa gtcttggcgc atcccacccc tagcaaatgg
      481 ctgcaaaagc ggttgtacga ttaccgcttc ttcctcgctt tcgccgaaca ggatgccgag
      541 gcgatgaa
//
LOCUS       DAXHNE010000282          535 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.282, whole genome shotgun sequence.
ACCESSION   DAXHNE010000282 DAXHNE010000000
VERSION     DAXHNE010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.282"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..>535
                     /locus_tag="ACFM4Z_002105"
     CDS             <1..>535
                     /locus_tag="ACFM4Z_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC6022070.1"
                     /translation="WLSDTPGQDAYSINLIEISVYYKKTDQGWVLEPYNQQNKAHFIQ
                     FLRDGLDSVDDIVIRKDACHLNQDLGKSLLDLGFKKIPSVYPEYEAYEDKRSIPENPY
                     IHELQYIKKGENPAIITHQNYHRYGENDYSTDIGSCINGFTVQYYPFIREKQQLTQQE
                     LVGYHQQVEQLVQSFVNN"
BASE COUNT          177 a           99 c          114 g          145 t
ORIGIN      
        1 atggctttcc gacacgcccg ggcaggatgc ttactccatt aatttgatag agattagcgt
       61 ctattacaaa aaaaccgacc aaggctgggt gctcgaacca tacaaccagc agaacaaagc
      121 acactttatt caatttctac gcgatggttt ggatagcgtg gacgatattg ttatccgaaa
      181 agatgcgtgt catttgaatc aagacttagg aaaatcgttg cttgatttgg gatttaaaaa
      241 aatcccgtct gtctatcctg aatatgaagc ttatgaagat aaaaggtcga ttcctgaaaa
      301 cccatatatt catgaactac agtatattaa gaaaggagaa aatccggcga ttattactca
      361 tcagaactat cataggtatg gagagaacga ttacagtact gatataggtt cctgtattaa
      421 cggtttcacg gtacagtatt acccgtttat tcgggaaaag cagcagctca cacagcagga
      481 gttggtaggt tatcaccaac aagtagagca attggtacag agttttgtaa acaat
//
LOCUS       DAXHNE010000283          527 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.283, whole genome shotgun sequence.
ACCESSION   DAXHNE010000283 DAXHNE010000000
VERSION     DAXHNE010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.283"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..188
                     /locus_tag="ACFM4Z_002106"
     CDS             <1..188
                     /locus_tag="ACFM4Z_002106"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6022071.1"
                     /translation="LDLIHYTCVMVSGTHHQPALHSVVLSGNCPVVSMGCPAAPCAAQ
                     RLCALGGRGWRPGGLLL"
BASE COUNT          110 a          123 c          146 g          148 t
ORIGIN      
        1 gtttagattt aattcactat acctgcgtaa tggtatcggg tactcatcat caacctgcct
       61 tgcattcggt tgtgttgtcc ggcaactgtc cggttgtgtc gatgggttgc cctgccgctc
      121 cctgtgctgc gcaacggttg tgtgccttgg gtgggcgcgg gtggcggccg ggcggtttgt
      181 tgctatgata cgggagttcc gatatacaag ggtgggcttc agcccaccaa tcaacgaccg
      241 aaaaagaaaa aacgttgggc tgaagcccac tctacgcaat tacgcaactg ttatcgtggg
      301 gatgacggga ttttaggttt ttgtttttct gtttttgcgg gaaggatggg ttttaggttg
      361 cggtcattta tcggaaaagc agaaatcgct ccgccgtcat tcccgcgaaa gtgggaatct
      421 gaacacgtcc gtagggaaac ctatatcccg tcattcccac gaaagtggga atctagaacc
      481 tctgaatttt cagacaacct ttgaatattg ccgccgtccc gcgttct
//
LOCUS       DAXHNE010000284          515 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.284, whole genome shotgun sequence.
ACCESSION   DAXHNE010000284 DAXHNE010000000
VERSION     DAXHNE010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.284"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
BASE COUNT          127 a          130 c          121 g          137 t
ORIGIN      
        1 atccatccat acggaaacct gcattccgtc attcccacga aagtgggaat ccaaaatccc
       61 ggactttcag ataatctttg aatattactg ttgttctaag gtctagattc ccgcctgagc
      121 gggaatgacg ggtcttttat aaactttgaa tattgctgtt atcccaaggt ctagattccc
      181 gcctgcacgg gaatgacggc atcggtttga cggtatttaa ttgaattgcg gaaattgatg
      241 gattcagtga gattggcggg atgaagccta ccctatagcc cgccttttac gaacccgccc
      301 cctcccgaaa aacgcaaaaa atgccgtccg aaaacctttc ggacggcatt ttcgcgtgca
      361 aatcagtaga agatttcacg ccagctgatt cgtttcatac cgcacgtcgg gccggtaatg
      421 tccaagctgt ccaaatcgtt catcagcagg gtgcgcaccc ctttttggga gaagcaacgg
      481 ttgtttttac cggaacgctt gccgtcgggg tctat
//
LOCUS       DAXHNE010000285          498 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.285, whole genome shotgun sequence.
ACCESSION   DAXHNE010000285 DAXHNE010000000
VERSION     DAXHNE010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.285"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..154)
                     /locus_tag="ACFM4Z_002107"
     CDS             complement(<1..154)
                     /locus_tag="ACFM4Z_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HFC6022072.1"
                     /translation="MQPTLTGSDTAAQQTLQGINHLGNLSPEAQLAAATALQDSAFAV
                     KDGINSA"
     gene            complement(217..>498)
                     /gene="imm40"
                     /locus_tag="ACFM4Z_002108"
     CDS             complement(217..>498)
                     /gene="imm40"
                     /locus_tag="ACFM4Z_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm40 family immunity protein"
                     /protein_id="HFC6022073.1"
                     /translation="IYSRQGESLIGIGIREAALPVPTAIDILNLFINERILVLGGDIY
                     IKKDNYFYQTYDNWYYEGSNLFNSINKAMHYLSQIKLENAYVSFVLKFI"
BASE COUNT          146 a           91 c           79 g          182 t
ORIGIN      
        1 tggcagagtt gataccgtct tttaccgcaa aagcactgtc ttgtaatgcg gttgcagccg
       61 caagttgtgc ttcgggactt aaatttccta aatgattaat accttgtaga gtctgctgag
      121 cggctgtatc tgagccggtc agtgtaggtt ggattaaaac ccagcatttt caacatcttg
      181 atgttttact ataaatctat tcttgtgctt cctttgttag ataaatttca acacaaaaga
      241 tacgtatgca ttctctaatt ttatttgaga taaataatgc attgctttgt tgatactgtt
      301 aaataaatta cttccctcgt aataccaatt atcatatgtt tgataaaaat aattatcttt
      361 cttgatataa atatctcccc ccaatacaag tattctttca tttataaata aatttaatat
      421 atctattgca gtggggacgg gtaatgcggc ttctctaatt ccaattccta tcaaagattc
      481 cccttgtctc gaataaat
//
LOCUS       DAXHNE010000286          495 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.286, whole genome shotgun sequence.
ACCESSION   DAXHNE010000286 DAXHNE010000000
VERSION     DAXHNE010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.286"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(137..>495)
                     /locus_tag="ACFM4Z_002109"
     CDS             complement(137..>495)
                     /locus_tag="ACFM4Z_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC6022074.1"
                     /translation="IPTKVGMTGFNDAAGVASSSTIKGKYVKEVEVKNGVVTATMLST
                     GVNNEIKGKKLSLWAKRQAGSVKWFCGQPVTRAANAKDDVAAATGTDKIDTKLKKPFY
                     PISFRTDEAGFPLLRE"
BASE COUNT          103 a          106 c          130 g          156 t
ORIGIN      
        1 agattttacg ttctggattc ctactttcgt gggaaagacg aattttaggt ttctgttttt
       61 ggttttctgt ccttgtggga atgatgaaaa tttaagtttt aggaatttat cggaaaaaac
      121 agaaaccgct ctgccgtcat tcccgcaaaa gcgggaatcc agcctcgtcg gtgcggaaac
      181 ttatcgggta aaaaggtttc tttagtttgg tgtcgatttt gtcggtgccg gtggcggcgg
      241 caacgtcgtc tttggcgttg gcggcgcgcg taaccggctg tccgcagaac cattttaccg
      301 aaccggcttg acgcttggcc cacagggaga gttttttgcc tttgatttcg ttgtttacgc
      361 cggttgaaag cattgtggcg gtaacgacgc cgtttttgac ttcaacttcc ttaacatatt
      421 tgcctttgat tgttgaagaa gatgccacgc cggcggcatc attaaatccc gtcattccca
      481 ctttcgtggg aatga
//
LOCUS       DAXHNE010000287          469 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.287, whole genome shotgun sequence.
ACCESSION   DAXHNE010000287 DAXHNE010000000
VERSION     DAXHNE010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.287"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            119..469
                     /locus_tag="ACFM4Z_002110"
                     /pseudo
     CDS             119..469
                     /locus_tag="ACFM4Z_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          132 a          137 c          111 g           89 t
ORIGIN      
        1 tcattcccac gcaggcggga atctaggtct gtcggcacgg aaacttatcg ggtaaaaagg
       61 tttctccggt cctgagttct agattcccgc ttttgcggga atgacgggat ttaatgatgc
      121 cgccggcgtg gcatcctcct ccgacatcaa aggcaaatat gttgagaaag ttgaagtcaa
      181 aaacggcgtc gttaccgcca caatgctttc aagcggcgta aacaaagaaa tcaaaggcaa
      241 aaaactctcc ctgtgggcca agcgtcaaaa cggttcggtc aaatggttct gcggacagcc
      301 ggttacgcgc aacgacaccg ccaccgccac cgacgtcgcc gccgccaacg gcaagaccga
      361 cgacaaaatc aacaccaagc acctgccgtc aacctgccgc gatgattcat ctaccggttg
      421 catagaaaca ccacgcgccg atttcaaaca ctttcaaaaa atcagccgt
//
LOCUS       DAXHNE010000288          452 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.288, whole genome shotgun sequence.
ACCESSION   DAXHNE010000288 DAXHNE010000000
VERSION     DAXHNE010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.288"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            41..452
                     /locus_tag="ACFM4Z_002111"
                     /pseudo
     CDS             41..452
                     /locus_tag="ACFM4Z_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          139 a          125 c          104 g           84 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat actttatgcc gctaccgcaa
       61 ctggcggcgc aagacgacac cctgtccgaa ttgctcaaac tgattaactg ccaactgacc
      121 gaaaaaggct taaaagtaga gaaagcatcc gccgctgtcg ttgacgccac cattattcag
      181 accgccggca gcaaacagcg tcaggctata gaagtcgatg aagaaggaaa agtcagcggc
      241 caaaccacac cgagtaaaga taaagatgcc cgccggacaa agaaaaacgg cctctacaga
      301 ctcagttaca aacaacatac ccgtaccgat gaggaaggct atatcgagaa actgcacatt
      361 acccccgcca atgcccatga gtgcaaacac ctgtcgccgt tgttggaagg gatagctgaa
      421 ggtacgaccg tctatgccga caaaggctac ga
//
LOCUS       DAXHNE010000289          445 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.289, whole genome shotgun sequence.
ACCESSION   DAXHNE010000289 DAXHNE010000000
VERSION     DAXHNE010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.289"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            26..190
                     /locus_tag="ACFM4Z_002112"
     CDS             26..190
                     /locus_tag="ACFM4Z_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6022075.1"
                     /translation="MGQVAFYEKMIGLWSSKSREASERADLAAFEFAEGELANYREML
                     KRHLQTKSVE"
     gene            193..429
                     /locus_tag="ACFM4Z_002113"
     CDS             193..429
                     /locus_tag="ACFM4Z_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6022076.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTFKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSFVSVKRQEVENESRETAGE"
BASE COUNT          107 a           89 c          144 g          105 t
ORIGIN      
        1 gtaaacgcaa gaatcgtcgt taattatggg acaggttgcg ttttacgaaa agatgattgg
       61 gctgtggtcg agcaaaagcc gtgaggcaag cgaacgggct gatttggcgg cgtttgaatt
      121 tgcggagggc gaactggcca attatcggga aatgctgaaa cggcacctgc aaaccaaaag
      181 tgtggaatag caatgcgtat tttggatatt tttaaaaacc ccgcgacagg caatgtgtcg
      241 cactcgaaac tgtgggcaaa cgttgcctgc gcggcgggga cgtttaagtt tgtgatgctg
      301 cccgatccgt cggcggagat ttgggcggtg tatttgggca ttgtcggcgg ctatgcggtg
      361 gcgcgctcgt ttgtcagcgt caaacgtcag gaggtcgaga atgaatctcg tgaaactgct
      421 ggcgaataac tggcaaccga ttgcc
//
LOCUS       DAXHNE010000290          441 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.290, whole genome shotgun sequence.
ACCESSION   DAXHNE010000290 DAXHNE010000000
VERSION     DAXHNE010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.290"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..376
                     /locus_tag="ACFM4Z_002114"
     CDS             <1..376
                     /locus_tag="ACFM4Z_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation factor"
                     /protein_id="HFC6022077.1"
                     /translation="AVKEDKVISADRYLEWVKKQFGRAANGLKFIFPELDKAKLFELI
                     EPSHHKLPKSLAPEAYDCAFLKAQAIHEQPAFKPYNDPYYMYEYYENLEKQLEQQKHV
                     NNEESYNNFIYDKFARLPISWA"
BASE COUNT          152 a          106 c           84 g           99 t
ORIGIN      
        1 cgcggttaag gaagataagg tcatttcagc cgaccgctac cttgaatggg taaaaaaaca
       61 gttcggacgt gcggcaaacg gtctgaaatt catttttccc gaattggaca aagccaaact
      121 gtttgaactg attgagccga gtcatcacaa gctgcccaag tctttggctc ccgaagccta
      181 cgactgcgcc tttttgaaag ctcaagccat tcatgaacag cccgcattca aaccgtacaa
      241 tgacccttac tatatgtacg aatattacga gaatcttgaa aaacagcttg aacagcaaaa
      301 acacgtcaac aatgaagaaa gctataacaa cttcatttac gacaaattcg caagactacc
      361 gatttcatgg gcttaaagtg tctgcccgaa agacgtttaa tcacacaagg aaaccaaaaa
      421 atgaacatcc aacttcaagg c
//
LOCUS       DAXHNE010000291          440 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.291, whole genome shotgun sequence.
ACCESSION   DAXHNE010000291 DAXHNE010000000
VERSION     DAXHNE010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.291"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(191..>440)
                     /locus_tag="ACFM4Z_002115"
     CDS             complement(191..>440)
                     /locus_tag="ACFM4Z_002115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6022078.1"
                     /translation="FARRGEKYSCYSPTYARFASILRPFMCNTLHLLNKIQTTLYIKC
                     KKYAVIPANAGIQTPRHGNLSSKTVSQILRSRFPRSRE"
BASE COUNT          132 a          109 c          112 g           87 t
ORIGIN      
        1 cgggaatcca gccccgtggg agcagaaact tacaccccgt cattcccgca aaagcgggaa
       61 tccagtaacc gaaaaaccac gggaatttat cggaaaaaca gaaacccccg accgtcattc
      121 ccgcgaacgc gggaatccag taaccgaaaa accacgggaa tctatcggaa aaaacagaaa
      181 ccctcgaccg tcattcccgc gaacgcggga atctagaacg tagaatctga gaaaccgttt
      241 tactcgataa atttccatgc cgaggggtct ggattcccgc gttcgcggga atgacggcat
      301 attttttgca tttgatataa agggtcgttt gaattttgtt cagcaagtgc aaagtgttgc
      361 acataaaagg gcgcaggata gaggcaaagc gggcgtaggt cgggctgtag caactgtatt
      421 tttcaccccg tcgggcaaaa
//
LOCUS       DAXHNE010000292          408 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.292, whole genome shotgun sequence.
ACCESSION   DAXHNE010000292 DAXHNE010000000
VERSION     DAXHNE010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.292"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..282)
                     /locus_tag="ACFM4Z_002116"
     CDS             complement(<1..282)
                     /locus_tag="ACFM4Z_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6022079.1"
                     /translation="MKYRRFYRNGGTYFFTVVTNKRQKILTDDAVRLALRQAVMAVRE
                     RYPFEILAWVLMPDHLHTIWRLPDNDSAYSERWRQIKRHSQYLIGGNLRL"
BASE COUNT          107 a           96 c           96 g          109 t
ORIGIN      
        1 aagcctgaga ttgccgccga ttaaatattg gctgtgccgc ttgatttgcc gccagcgttc
       61 cgaataagca gaatcattgt ccggcagccg ccatatggta tgcagatggt cgggcatcaa
      121 cacccatgcc aaaatttcaa acggataccg ttcgcgcacc gccattaccg cctgccgtaa
      181 agccaaacgc accgcatcat cggtcaaaat cttctgccgt ttattggtta caaccgtaaa
      241 aaagtaagtg ccgccattgc ggtaaaaacg acggtatttc atagtattat gctcggaatg
      301 attttgtagg tcggattctt gaattcgaca ttttgggcat tgctgcaatg gattgcaatg
      361 atgggaatgt taaaggtttt gtcggataca agtatccgac ctacgctt
//
LOCUS       DAXHNE010000293          401 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.293, whole genome shotgun sequence.
ACCESSION   DAXHNE010000293 DAXHNE010000000
VERSION     DAXHNE010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.293"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            108..323
                     /gene="rpmE"
                     /locus_tag="ACFM4Z_002117"
     CDS             108..323
                     /gene="rpmE"
                     /locus_tag="ACFM4Z_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFC6022080.1"
                     /translation="MKQGTHPNYHEVNVTCSCGNKFVTKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT          124 a          104 c           74 g           99 t
ORIGIN      
        1 attttatcga atatcctctt gacgaaacaa cagaaaatag cgagaatgcc gagcttgtct
       61 gaagcatagg tacatcccct atgcccgctt ttttgaaagg aaatcaaatg aaacaaggca
      121 ctcacccgaa ttatcacgaa gtcaacgtta cctgctcctg cggcaacaaa tttgtaacca
      181 aatccgcgat ggaaaaagaa aacttcaata tcgaggtttg ctccctgtgc cacccgttct
      241 ataccggcac ccaaaaaatc gtcgatacca ccggccgcgt ggacaaattc aacaacaaat
      301 tcggcaacct gttcaaacgc tgattgccgc tttgcaagaa ttaagccctg ccatcatttc
      361 gatggcaggg ctttttaata tttggcacac tgacttctta t
//
LOCUS       DAXHNE010000294          397 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.294, whole genome shotgun sequence.
ACCESSION   DAXHNE010000294 DAXHNE010000000
VERSION     DAXHNE010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.294"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            44..241
                     /locus_tag="ACFM4Z_002118"
     CDS             44..241
                     /locus_tag="ACFM4Z_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6022081.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
BASE COUNT          114 a           90 c           85 g          108 t
ORIGIN      
        1 ctgaaagtca tcggttttca actcgtgaag aaaggctgat tgaatgcaga aagtctatgt
       61 tgtccagtcc gtatcaacag gggactttct gtatctctct cctgaaacgg gcgacatcgg
      121 acataccaaa ttaatcacca atgccgatta tttctacgac ttcgaagaag cgattaacgc
      181 aggtttggaa gaaatcggca accaatacga atttgtcgta ttcggatttt tgaaagactg
      241 attttcggat gttcggcggt cgtctgaaaa acgctccatc cattaccgcc aaacactttt
      301 tgaaggaaaa tatcatgaaa tttattaaca cctgccgtaa atacggcgca aaactggctg
      361 ttgtaacagc tgctcccctg gctttggccg cacatgc
//
LOCUS       DAXHNE010000295          382 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.295, whole genome shotgun sequence.
ACCESSION   DAXHNE010000295 DAXHNE010000000
VERSION     DAXHNE010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.295"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            123..302
                     /locus_tag="ACFM4Z_002119"
     CDS             123..302
                     /locus_tag="ACFM4Z_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6022082.1"
                     /translation="MFWYVIGFCAFVVALLSLWVNAGAFGMQEDDTPQSDYERRLGLG
                     AKLKNKNPDTDEKRQ"
BASE COUNT           98 a           71 c          102 g          111 t
ORIGIN      
        1 atttgtactg tcttcggctc gccgccttgt cctgatttaa atttaattca ctatatttgc
       61 ccgtgcgttt gtgataaatt cggcactgta tttgatatga ttttaacaaa tcgggagggg
      121 caatgttttg gtatgtgatt ggcttttgcg cctttgtcgt cgcgctgctg tcgctgtggg
      181 tcaatgccgg cgcgttcggt atgcaggaag acgatactcc gcaatcggat tatgaaaggc
      241 gtttgggctt gggtgcgaag ctgaaaaaca aaaatccgga tacggacgaa aaacggcaat
      301 aaagacaaca aatgccgtct gaaaggtttt cagacggcat ttgctttgga gcggatatag
      361 tggattaaca aaaatcagga ca
//
LOCUS       DAXHNE010000296          379 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.296, whole genome shotgun sequence.
ACCESSION   DAXHNE010000296 DAXHNE010000000
VERSION     DAXHNE010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.296"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
BASE COUNT          117 a          108 c           81 g           73 t
ORIGIN      
        1 caaagccaaa gacgacgccg tcaccggcaa gggcaccgcc aacatcaaca ccaagcacct
       61 gccgtcaacc tgccgcgatg attcaactgc cagctaaggt aaattatacc ataaatttta
      121 aataaatcaa gcggtaagtg attttccacg gccactcgga ttaatccggg tggcttcatt
      181 ttaaaggttt gcaaggcagg cgggggcgtc cgtttaaaca cggggtttat ctgacaggcg
      241 cacaatccgc cctctcattt accgaacaag cggactagat tcccgcctac tcgggaatga
      301 tgaagctatc cgcacggaaa cctgcaccac gtcattccca caaggacaga aaaccaaaaa
      361 cagaaaccta aaattcgtc
//
LOCUS       DAXHNE010000297          365 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.297, whole genome shotgun sequence.
ACCESSION   DAXHNE010000297 DAXHNE010000000
VERSION     DAXHNE010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.297"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(152..>365)
                     /locus_tag="ACFM4Z_002120"
     CDS             complement(152..>365)
                     /locus_tag="ACFM4Z_002120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6022083.1"
                     /translation="VKEYQPPVGIFLFVCFEKCLFPDFAIPIRFCAVRCVLARTCSKS
                     HVIPVFEALIRDADFQYIFSATTTKA"
BASE COUNT          122 a           86 c           61 g           96 t
ORIGIN      
        1 gtcttttcta aaatatgcag aaattcgaat atcgaatttg ccttatgatt acggttttct
       61 gtcttatctg ccttaaaacg ccggtattcc gtttgaacca aattatattt gccgaaacgc
      121 tcaaaaatcc cccttacctg cccgacagac atcaagcctt cgttgttgta gctgagaaaa
      181 tatattgaaa gtccgcatcc ctaatcaaag cctcaaatac cggaatcaca tgcgattttg
      241 agcaagtccg cgctaaaaca catcgtacag cgcaaaaccg gatgggaatg gcaaaatccg
      301 gaaacaaaca tttttcaaag catacaaaca aaaaaatacc aaccggaggt tggtattcct
      361 taact
//
LOCUS       DAXHNE010000298          364 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.298, whole genome shotgun sequence.
ACCESSION   DAXHNE010000298 DAXHNE010000000
VERSION     DAXHNE010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.298"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..283)
                     /locus_tag="ACFM4Z_002121"
     CDS             complement(<1..283)
                     /locus_tag="ACFM4Z_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFC6022084.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPVIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPAGDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYVPS"
BASE COUNT           97 a           92 c          108 g           67 t
ORIGIN      
        1 aagaaggaac gtatgtaccc gaaacggcag gtgcagacgg cggaacataa gcaggaggcg
       61 tataaaccgg cgcgctttgc accggcggca cataaggcgc atcgccggca ggagccgggc
      121 tgtacggcgt tgctccatag gggttgttgt aaactgccga agacggcgcg tcctgcatac
      181 ctgaattgcc tgcaatgacg ggggcaggct gctgggtagc acaaccgccc aacagtgcgg
      241 caaaggaggc gcaagccgcc aaaagtgtcg tttgtttcaa cataagataa ccttcatgtt
      301 ccgatatata gcctgaatgc ggtatatcat aataaaaatg cgcgttcttc tcaagcgcaa
      361 agcc
//
LOCUS       DAXHNE010000299          363 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.299, whole genome shotgun sequence.
ACCESSION   DAXHNE010000299 DAXHNE010000000
VERSION     DAXHNE010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.299"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..>363
                     /locus_tag="ACFM4Z_002122"
     CDS             <1..>363
                     /locus_tag="ACFM4Z_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6022085.1"
                     /translation="DYSTLCRYRNWLAQDDTLSELLKLINRQLTEKGLKVEKASAAVV
                     DATIIQTAGSKQRQAIEVDEEGQVSGQTTPSKDKDARWTKKNGLYRLGYKQHTRTDEE
                     GYIKKLHITPANTHECKHL"
BASE COUNT          124 a          105 c           80 g           54 t
ORIGIN      
        1 gattacagca ccttatgccg ctaccgcaac tggctggcgc aagacgacac cctgtccgaa
       61 ttgctcaaac tgattaaccg acaactgacc gaaaaaggcc taaaagtaga gaaagcatcc
      121 gccgccgtcg ttgacgccac cattattcag accgccggca gcaaacagcg ccaggccata
      181 gaagtcgacg aggaaggaca agtcagcggc caaaccacac cgagtaaaga taaagatgcc
      241 cgttggacaa agaaaaacgg tttatacaga ctcggttaca aacaacatac ccgtaccgat
      301 gaggaaggct atatcaagaa actgcacatc accccagcca atacccatga gtgcaaacac
      361 ctg
//
LOCUS       DAXHNE010000300          353 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.300, whole genome shotgun sequence.
ACCESSION   DAXHNE010000300 DAXHNE010000000
VERSION     DAXHNE010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.300"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
BASE COUNT           89 a           69 c           79 g          116 t
ORIGIN      
        1 tgggtgtttc gggaaactta taaatcgtca ttcccgcgca ggcgggaatc cggtttgctc
       61 ggtttcggtt tttcgggcgt ttcgggaaac tgatgaatcg tcattcccgc gcagatgata
      121 tgttgcccgt caacacaaaa taaaaacaaa gctgcaacat actgatttat attgttattt
      181 ttatttacgt ttatttacga tatgcaaatg cacggttaca caaatatatt cgcgtaaccg
      241 tttaattttg ttgaatctta ttgattcaat cggtgtcttt ccgcatcgta aggctggccg
      301 gttttaacaa tgtaataggc gagcttcgcc agtttgcgca tgatggcaac gat
//
LOCUS       DAXHNE010000301          344 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.301, whole genome shotgun sequence.
ACCESSION   DAXHNE010000301 DAXHNE010000000
VERSION     DAXHNE010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.301"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
BASE COUNT          101 a           91 c           90 g           62 t
ORIGIN      
        1 gcttccttcg aaagctccgt tttcagacga ccttttggaa caccacaggc acacgcatcg
       61 aacggctgaa tcaaagattc agaccgatgg cagtccgcac ccgagtttat gcggcaaaca
      121 gcgaggctac ggcaacccgc cccctctccc tgtgggagag ggttagggag agggcagtaa
      181 gccgtaggct tacatcaaag ccgataacgc tttcacaaag cctgtgtgca ctgaaaactg
      241 aaaggtcgtc tgaaaacgga gctttcgaag gaagcagagt ttctgcgaag ctaaaaccga
      301 agctgcaaaa aatcgaaaag cagcctgcac gttgaaaatg ccgc
//
LOCUS       DAXHNE010000302          343 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.302, whole genome shotgun sequence.
ACCESSION   DAXHNE010000302 DAXHNE010000000
VERSION     DAXHNE010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.302"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..>343)
                     /locus_tag="ACFM4Z_002123"
     CDS             complement(<1..>343)
                     /locus_tag="ACFM4Z_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC6022086.1"
                     /translation="ELKAINLYDCAFEDFVPEIKDNFCVGIDLDIGTDDSDAADIFSV
                     RICSPKWILHNCFQNQRVKWGAGMMIMNEFNHSVIKSEIEKILKECSKETWEKSLTYL
                     LRFFSWEFEDY"
BASE COUNT          123 a           69 c           41 g          110 t
ORIGIN      
        1 tgataatctt caaattccca cgaaaaaaaa cgaagtaaat aagtcaatga cttttcccaa
       61 gtttcttttg aacattcttt aagaattttc tcaatttccg atttaataac agaatgatta
      121 aattcattca taatcatcat acccgctccc catttaaccc tttgattttg gaaacaatta
      181 tgcaaaatcc atttaggaga gcatatgcga acagaaaata tatctgcagc atcactatca
      241 tcagttccta tgtctaaatc aattcccaca caaaaattgt ctttgatttc gggaacgaaa
      301 tcttcaaagg cacaatcgta aagattgatg gctttcaatt cta
//
LOCUS       DAXHNE010000303          333 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.303, whole genome shotgun sequence.
ACCESSION   DAXHNE010000303 DAXHNE010000000
VERSION     DAXHNE010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.303"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
BASE COUNT           96 a           73 c           72 g           92 t
ORIGIN      
        1 ttttttgaat ttcgggcaac gcttgaatct tcattccgcg caggcggaaa ttatcggtgc
       61 ggtacggcaa cttttttcga tatgaaaaga ccgtcattcc tgtaaaaaca aaaaatcaaa
      121 aacagaaaat tgaaattcgt cattcccgcg caggcgggaa tctaggacgt aaaatctata
      181 gtggattaac aaaaaccagt acagcgttgc cttgacttag ctcgaagaga acgattctct
      241 aaggtgctga agcaccgagt gagtcggttc cgcactatcc gtactgtctg cggcttcgtc
      301 gccttgtcct gatttttgtt aatccactat aaa
//
LOCUS       DAXHNE010000304          322 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.304, whole genome shotgun sequence.
ACCESSION   DAXHNE010000304 DAXHNE010000000
VERSION     DAXHNE010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.304"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(135..>322)
                     /locus_tag="ACFM4Z_002124"
     CDS             complement(135..>322)
                     /locus_tag="ACFM4Z_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6022087.1"
                     /translation="TIRDEADFAWHFDYLHFNPVKHGYVGQISDWGFSTFHRYVKQDI
                     YPHNWGGGNADFSIGYD"
BASE COUNT           85 a           80 c           71 g           86 t
ORIGIN      
        1 ttcgacaaat atcggttgcg tttggtttgc gcctccgtca gcggacggtt gcggcaggct
       61 ttgcgcataa tgccgttctg caaccgatgc tctttcagtt ttccgtaggt cggattctcg
      121 aatccgacat tacttcaatc gtatccaata gaaaagtccg cattgccgcc accccaatta
      181 tgcggataaa tatcctgttt gacataacgg tgaaacgtag aaaaccccca atcggaaatt
      241 tgtcctacat agccatgttt gaccggattg aaatgcagat aatcaaaatg ccaggcaaaa
      301 tcggcctcat cgcggatagt at
//
LOCUS       DAXHNE010000305          322 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.305, whole genome shotgun sequence.
ACCESSION   DAXHNE010000305 DAXHNE010000000
VERSION     DAXHNE010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.305"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(135..>322)
                     /locus_tag="ACFM4Z_002125"
     CDS             complement(135..>322)
                     /locus_tag="ACFM4Z_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6022088.1"
                     /translation="TIRDEADFACHFDYLHFNPVKHGYVGQISDWGFSTFHRYVKQGI
                     YPHNWGGGNADFSIGYD"
BASE COUNT           87 a           78 c           70 g           87 t
ORIGIN      
        1 ttcgataaat atcggttacg tttggtttgc gtttccgtca gcggacggtt gcggcaggct
       61 ttgcgcataa tgccgtcctg cagccgatgc tctttcagtt ttccgtaggt cggattctcg
      121 aatccgacat tacttcaatc atatccaata gaaaagtccg cattgccgcc accccaatta
      181 tgcggataaa taccctgttt aacataacgg tgaaacgtag aaaaccccca atcggaaatt
      241 tgtcctacat agccatgttt gaccggattg aaatgcagat aatcaaaatg gcaggcaaaa
      301 tcggcctcat cgcggatagt at
//
LOCUS       DAXHNE010000306          318 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.306, whole genome shotgun sequence.
ACCESSION   DAXHNE010000306 DAXHNE010000000
VERSION     DAXHNE010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.306"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
BASE COUNT           88 a           70 c           88 g           72 t
ORIGIN      
        1 gggaatgacg ggatttgaga ttgcggcatt tatcgggagc aacagaaacc gctccgccgt
       61 cattcccgcg caggcgggaa tctagacctt agaacaacag caatattcaa aggttagctg
      121 aagctttaga gattctagat tcccactttc gtgggaatga cggaatgtag gttcgtggga
      181 atgacgcggt gcaggtttcc gtatggatgg gttcgtcatt cccgcgcagg cgggaatcta
      241 gaccttagaa caacagcaat attcaaagat tataaaagac ctgtcattcc cgcgcaggcg
      301 ggaatccaga ccttagaa
//
LOCUS       DAXHNE010000307          317 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.307, whole genome shotgun sequence.
ACCESSION   DAXHNE010000307 DAXHNE010000000
VERSION     DAXHNE010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.307"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..>317
                     /locus_tag="ACFM4Z_002126"
     CDS             <1..>317
                     /locus_tag="ACFM4Z_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6022089.1"
                     /translation="MDTERQDPATKEALQYRTALFAGYESLTSRPLCTQTAIMVCNAI
                     KHPYEMAIRKTGGTALKGGNSGNVVYTPPEGEETIRGKLANWERFIHESGDLDPLIIM
                     AAA"
BASE COUNT           93 a           96 c           79 g           49 t
ORIGIN      
        1 atggatacgg aacggcaaga ccctgccacg aaagaagccc tgcaataccg caccgccctg
       61 tttgcaggct atgaatcact gacgagccgc cctttatgca cacaaaccgc catcatggtc
      121 tgcaacgcca tcaagcaccc ctacgaaatg gccatccgca aaacaggcgg cacagcccta
      181 aaaggaggca acagcggaaa tgttgtctat accccgcccg aaggagaaga aaccatacgc
      241 ggcaagctgg caaattggga gcggtttatt cacgaaagcg gcgatttaga cccgcttatc
      301 atcatggcgg cggcaca
//
LOCUS       DAXHNE010000308          310 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.308, whole genome shotgun sequence.
ACCESSION   DAXHNE010000308 DAXHNE010000000
VERSION     DAXHNE010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.308"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            3..>310
                     /locus_tag="ACFM4Z_002127"
     CDS             3..>310
                     /locus_tag="ACFM4Z_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6022090.1"
                     /translation="MAVSHHQGYKSAFAKQQAVIDKMEKDKAQAMLLSAQNYARELEQ
                     ARAEAKKYEVKAHAVGMALAKKQAEVSRLKTERDLCKIPFPPDSRNPNTGFRLFSPQI
                     P"
BASE COUNT           89 a           81 c           83 g           57 t
ORIGIN      
        1 tcttggcggt gtcgcaccat caaggctaca agtcggcttt tgcgaagcag caggcggtca
       61 ttgacaagat ggaaaaagac aaagcgcaag ccatgctgtt gtcggctcaa aactatgcac
      121 gcgagctgga acaggcgcgt gcggaagcga aaaaatatga agtcaaggcg cacgccgtcg
      181 gcatggcttt ggcgaaaaaa caggcggaag tcagccgtct gaaaacggaa agagaccttt
      241 gcaaaattcc tttccctccc gacagccgaa atcccaacac aggttttcgg ctgttttcgc
      301 cccaaatacc
//
LOCUS       DAXHNE010000309          308 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.309, whole genome shotgun sequence.
ACCESSION   DAXHNE010000309 DAXHNE010000000
VERSION     DAXHNE010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.309"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
BASE COUNT           83 a           64 c           65 g           96 t
ORIGIN      
        1 aaaggtctca aaccggtttg tcagggttta atggtcagcg cgtcctgcgt tgccatttgt
       61 tgtagtagta ttcgtaatct tcatcacgac cgcccgattc gatccaatta tcgtattcgg
      121 ggctgttttc aaagctgtca gacattatcc gtacctcgca aaatgggttt atcgatactt
      181 ttttagttat ggaatcaacc ataactaaaa agaagtatac ttctttgctt ttccgccaaa
      241 atatttaacc gcacatccgc tgcagggtaa aaaatggaag ttcaacgata ttgatacggg
      301 tatgggcg
//
LOCUS       DAXHNE010000310          308 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.310, whole genome shotgun sequence.
ACCESSION   DAXHNE010000310 DAXHNE010000000
VERSION     DAXHNE010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.310"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            18..212
                     /gene="thiS"
                     /locus_tag="ACFM4Z_002128"
     CDS             18..212
                     /gene="thiS"
                     /locus_tag="ACFM4Z_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFC6022091.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQKPFAVAVNTVFIPKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT           85 a           90 c           77 g           56 t
ORIGIN      
        1 agaaacatcg agaaaccatg aacatcatct taaacggcgg acccgccgaa cttcacggca
       61 caagcgttgc cgacctcatc gcccaaaccg cgccgcaaaa gccctttgcc gtggcggtca
      121 acaccgtttt catccccaaa ggcgcgtatg cggaaacggt tttgaacgaa aacgacaaaa
      181 tcgatatcgt gcggccggtg gtcggcggct aggcggtttt gccttttcag acgacccctg
      241 tccccaaaac aacgttatgg tggattaact ttaaatcagg acaaggcgac gaagccgcag
      301 acagtacg
//
LOCUS       DAXHNE010000311          302 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.311, whole genome shotgun sequence.
ACCESSION   DAXHNE010000311 DAXHNE010000000
VERSION     DAXHNE010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.311"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..300
                     /locus_tag="ACFM4Z_002129"
     CDS             <1..300
                     /locus_tag="ACFM4Z_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6022092.1"
                     /translation="KGYDSEENRQHLEEHQLLDGIMRKAHRKHPLSEVQTKRNRYLSK
                     TRYVVEQSFGTMHRKFRYARAAYFGLSKVSAQSHLKAMCLNLLKAANRLSAPAAA"
BASE COUNT           88 a           76 c           80 g           58 t
ORIGIN      
        1 aaaggctacg acagtgagga aaaccggcaa catctggaag agcatcagtt gttagacggt
       61 attatgcgca aagcccaccg caaacatcca ctgtcggaag tgcaaaccaa acgcaaccga
      121 tatttgtcga agacccgtta tgtggtcgaa cagagcttcg gtacgatgca ccgtaaattc
      181 cgctatgcgc gggcagccta tttcgggctg agcaaagtga gtgcacaaag ccatctgaag
      241 gcgatgtgtt tgaacctttt gaaagcggcc aacaggctaa gtgcgcccgc tgccgcctaa
      301 aa
//
LOCUS       DAXHNE010000312          295 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.312, whole genome shotgun sequence.
ACCESSION   DAXHNE010000312 DAXHNE010000000
VERSION     DAXHNE010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.312"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..>295
                     /locus_tag="ACFM4Z_002130"
     CDS             <1..>295
                     /locus_tag="ACFM4Z_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011145223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFC6022093.1"
                     /translation="IYRYLRQDKSNGSTLWQHLRICSKPYRKRYGSTWTRGKVPNRVG
                     IENRPAIVDQKSRIGDWEADTIVGKGQKSALLTLVERVTRYTIICKLDSLKAED"
BASE COUNT           92 a           89 c           66 g           48 t
ORIGIN      
        1 catttaccgc taccttcgcc aagacaaaag caacggcagc acgttgtggc aacatctcag
       61 aatatgcagc aaaccctacc gcaaacgcta cggcagcaca tggaccagag gcaaagtacc
      121 caaccgtgtc ggcatagaaa accgacccgc tatcgtcgac cagaaatccc gtatcggcga
      181 ttgggaagcc gacaccattg tcggcaaagg acagaaaagc gcattattga ccttggtcga
      241 acgcgttacc cgctacacca tcatctgcaa attggatagc ctcaaagccg aagac
//
LOCUS       DAXHNE010000313          280 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.313, whole genome shotgun sequence.
ACCESSION   DAXHNE010000313 DAXHNE010000000
VERSION     DAXHNE010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.313"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
BASE COUNT           94 a           45 c           47 g           94 t
ORIGIN      
        1 tttaaaattt cggaaaattt aaagatgttt ccagtatata tttacgccgt gtatatatca
       61 aggatatatg tttaaaaatt tggcttttgt aaatatatgg gcggtaaaac cgcattattt
      121 tatgttgtta cctattttac accttgtctg cacacgattt ccaaacaacc taaaaagaaa
      181 cctgccgaaa ggcaggttta aagaggtcat tttaaatgtg aactaaagtt tacaccctcg
      241 gagggtatcg cgtatatcag acgtacttaa tatacactat
//
LOCUS       DAXHNE010000314          272 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.314, whole genome shotgun sequence.
ACCESSION   DAXHNE010000314 DAXHNE010000000
VERSION     DAXHNE010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.314"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
BASE COUNT           95 a           73 c           45 g           59 t
ORIGIN      
        1 tgttaaacca ctataagaat gcaaacgaaa agcctcagta ttatccgaga caaccgcaaa
       61 ctttccatcg tcattcccac gaacccacat cccgttatta ccacgaaagc gggaatctag
      121 gactcagggt taagaaaacc tacatcccgt cattccctca aaaacagaaa accaaaatca
      181 gaaacctaaa atcccgtcat tcccacgaaa gtgggaatct aggtctgtcg gtgcggaaac
      241 ttatcggata aaacggtttc tttagattcc ac
//
LOCUS       DAXHNE010000315          269 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.315, whole genome shotgun sequence.
ACCESSION   DAXHNE010000315 DAXHNE010000000
VERSION     DAXHNE010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.315"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            <1..264
                     /locus_tag="ACFM4Z_002131"
     CDS             <1..264
                     /locus_tag="ACFM4Z_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFC6022094.1"
                     /translation="FYQHTKITKALKAETYFCRPYHSWEKGLNENTNGLIRQYFPKQT
                     DFRNISDREIRRVQDELNHRPRKTLGYETPSVLFLNLFQPLIH"
BASE COUNT           89 a           67 c           51 g           62 t
ORIGIN      
        1 ttctaccaac acaccaaaat aaccaaagca ttgaaagcgg agacttattt ttgtcgccct
       61 taccattctt gggagaaagg gctgaatgag aacaccaacg gactcatccg gcaatacttc
      121 cccaaacaaa ccgatttccg taacatcagt gatcgggaga tacgcagggt tcaagatgag
      181 ttgaaccacc gaccaagaaa aacacttggc tacgaaacgc caagtgtttt attcttgaat
      241 ctgttccaac cactaataca ctagtgttg
//
LOCUS       DAXHNE010000316          266 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.316, whole genome shotgun sequence.
ACCESSION   DAXHNE010000316 DAXHNE010000000
VERSION     DAXHNE010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.316"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
BASE COUNT           72 a           58 c           72 g           64 t
ORIGIN      
        1 gataagtttc cgcaccgaca aaactagatt cccactttcg tgggaatgac gggatgcagg
       61 ttcgtgggaa tgacgcgaac agaaacctca aatcccgtca ttcccgcgca ggcgggaatc
      121 tagaccttag aacaacagca atattcaaag attatctgaa agtctgggat tctggattcc
      181 cactttcgtg ggaatgacgg aatgtaggtt cgtaggaatg acgggatgta ggtttccgta
      241 tggatggatt cgtcattccc gcgcag
//
LOCUS       DAXHNE010000317          263 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain M01 240286
            SAMEA1706872-rid23337753.denovo.317, whole genome shotgun sequence.
ACCESSION   DAXHNE010000317 DAXHNE010000000
VERSION     DAXHNE010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1706872
            Sequence Read Archive: ERR484231
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002378219.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002378219.1
            Genome Coverage       :: 184x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 08:09:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,132
            CDSs (total)                      :: 2,076
            Genes (coding)                    :: 1,972
            CDSs (with protein)               :: 1,972
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 55 of 104
            Pseudo Genes (internal stop)      :: 16 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1706872-rid23337753.denovo.317"
                     /strain="M01 240286"
                     /db_xref="taxon:487"
     gene            complement(<1..>263)
                     /locus_tag="ACFM4Z_002132"
     CDS             complement(<1..>263)
                     /locus_tag="ACFM4Z_002132"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6022095.1"
                     /translation="RNLKFVIPTNLHPVIPTKVGIQFFEFQSFPINCLSIECLDSRLR
                     GNDGGAVSIFSGKYPQAKILLFSQKQKTKNRNLKFVIPAQAGI"
BASE COUNT           67 a           50 c           68 g           78 t
ORIGIN      
        1 gattcccgcc tgcgcgggaa tgacgaattt caggtttctg tttttggttt tctgtttttg
       61 tgaaaataac aggattttag cttgtgggta tttaccggaa aaaatagaaa ccgctccgcc
      121 gtcattcccg cgcaggcggg aatccagaca ttcaatgctg aggcaattta tcggaaatga
      181 ctgaaactca aaaaactgga ttcccacttt cgtgggaatg acgggatgta ggttcgtggg
      241 aatgacgaat ttcaggtttc tgt
//
